LOCUS       DATMZD010000001         2423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_60357,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000001 DATMZD010000000
VERSION     DATMZD010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60357"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /gene="rlmN"
                     /locus_tag="VN478_00005"
     CDS             <1..802
                     /gene="rlmN"
                     /locus_tag="VN478_00005"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0002935 - tRNA
                     (adenine(37)-C2)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0070040 - rRNA
                     (adenine(2503)-C2-)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HWQ21097.1"
                     /translation="PRTVCVSTEAGCQANCAFCATGHLGFTRVLTPSEIVEQVLHFAR
                     VLKSNGERVTNVVFMGEGEPFLNYDNVKNAILLLNDKDGFNLGQRRISVSTSGIIPGI
                     ERFAAEDWQVNLAISLHAPVNGLRSILMPINRTYPLEQLMPALDAYVAKTNRKLIIEY
                     LMLGGLNDSAHNADALADLIHKYKEVLRLSVVNLIVYNPVKDSSGRPDRFRAPRPASL
                     RVFEETLTKREIDWTRRASFGSDISGACGQLAGEAAPDTEGMGDLMST"
     gene            854..1363
                     /locus_tag="VN478_00010"
     CDS             854..1363
                     /locus_tag="VN478_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005812934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HWQ21098.1"
                     /translation="MCKIVKVYRESLPELRLIGKRYVDGDRGKDGGFSSQWEEWFRRG
                     WFKPLEALGSLRENEDAYVGCMRCAGEFEYWIGMFFPIDTPVPDGYSSVDIPAGDVGT
                     CWIHGRAETGELYGQATHDACVSRIREAGWQPADNAWVFERYTCPRFTAPDEAGRVIL
                     DYCIYLKKE"
     gene            1574..2068
                     /locus_tag="VN478_00015"
     CDS             1574..2068
                     /locus_tag="VN478_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017471452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21099.1"
                     /translation="MSYQTKRTITSMVSGVLVLVAYCIHAFGRYSSGAIADGDLKFWA
                     GTMLVFIGIGIVATIILQIIFHIGLSIGIAVKDRACDEKEIDKRIEATVIEDEMDKLI
                     ELKSSQIGFVLAGVGFVGALVLLLFGYSTVVMLNVMYLSFLVGSLMEGAASLYFYGKG
                     VSNA"
     gene            2061..2288
                     /locus_tag="VN478_00020"
     CDS             2061..2288
                     /locus_tag="VN478_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWQ21100.1"
                     /translation="MPKKLEVTNNIRTLRFFASEMTQQALAEKAGVSRQTIIAVEAGK
                     YSPTLELAFRIADAFGLPITEVFGYKTKGST"
BASE COUNT          552 a          659 c          725 g          487 t
ORIGIN      
        1 cccccgtaca gtgtgcgtgt ccaccgaagc cggctgccaa gccaattgcg cgttctgcgc
       61 gacgggtcac ctgggcttca ccagggtcct cacaccctcc gagatcgtcg agcaggtgct
      121 gcacttcgcc cgcgtgctga agtccaatgg cgaaagggtc accaacgtgg tcttcatggg
      181 cgagggtgaa cccttcctca actacgacaa cgtcaagaac gccatcctgc tgctgaacga
      241 caaggacggc ttcaacctgg gacagcggcg catctcggtc tccacgagtg gcatcattcc
      301 gggtattgaa cgcttcgctg ccgaagactg gcaggtcaac ctggcgatct ccctgcatgc
      361 ccccgtcaac ggcctgcgat cgatcctgat gccgatcaac cggacctatc cgctggagca
      421 gctaatgccc gctcttgacg catatgtcgc gaagacgaac aggaagctga ttatcgagta
      481 cctgatgctt gggggactca atgacagcgc gcacaacgcc gacgcgctgg ccgacctcat
      541 ccacaagtac aaagaggtcc ttcgcctgag cgtggtcaac ctgattgtct ataacccggt
      601 caaagacagc agcgggcgcc ccgacaggtt cagggctccc aggcccgcgt cgctgcgagt
      661 gttcgaggag accctgacga agcgtgagat cgactggact cgcagagcca gctttggaag
      721 cgacatcagc ggcgcctgcg gacagctggc aggtgaggcg gcgccggaca cggaaggaat
      781 gggggatttg atgtcgactt gagagtgctg cattctgagc caagggcacg atgtcaacga
      841 gtggaggcca cagatgtgca agatcgtcaa ggtgtacagg gagagccttc cagagcttcg
      901 tctgataggc aagcgatacg tcgacggaga cagaggcaag gacggcggct tcagcagcca
      961 gtgggaggag tggtttcgac ggggctggtt caagccgttg gaagcattgg gaagccttcg
     1021 cgagaacgaa gacgcatacg tgggctgcat gcggtgtgcg ggtgagttcg agtactggat
     1081 cggcatgttc tttcccatcg atacgccagt tccggatggc tattcgtccg tcgatatccc
     1141 tgcgggggat gtaggcacat gctggatcca cgggcgcgct gagaccggcg aactctacgg
     1201 tcaggcgacc catgacgcct gcgtctccag gatcagagag gcgggctggc agcctgccga
     1261 caacgcctgg gtctttgagc gctacacttg cccccgcttc acggcgcccg acgaagcagg
     1321 cagggtgatc ctcgattact gcatctatct gaagaaggaa tagctgcagc aacagtccct
     1381 gccgagccgc cttcttcaga taccttcgcc acgttccact ggacatccgt cgcaagcgct
     1441 tccgcaacaa ggcggagtgg caggaacgat atgagcaata tcgtcagtca tggaaagtgc
     1501 tattgacatg gagaataaca ttccatacaa tatgtctgct agacattaca tattgtaaag
     1561 gagagtgttg accatgtcat accaaaccaa gaggacaata acaagcatgg tgtcgggagt
     1621 gctggttctt gtcgcctact gcatccatgc attcggtcgc tattcctcgg gagcaattgc
     1681 ggacggagat ctgaagttct gggccgggac tatgcttgtc ttcatcggta tcgggatcgt
     1741 agcgaccatt atcctccaga tcatcttcca tatcggcctt tcgatcggca ttgccgtcaa
     1801 agaccgggca tgcgacgaga aggagattga caagaggatt gaagccaccg tgatcgagga
     1861 tgagatggac aagctgatcg agctgaagtc gtcgcagata ggcttcgttc tcgccggtgt
     1921 cggatttgtc ggcgcgctgg tcctcctgct tttcggctat tcaaccgtcg tcatgctgaa
     1981 cgtgatgtac ctttcgttcc ttgtgggatc gttgatggaa ggcgcggcaa gcctgtactt
     2041 ctatggcaag ggtgtcagca atgcctaaga agctcgaagt cacgaacaac atccgtacgc
     2101 ttcgtttctt tgccagcgag atgacccagc aggcactcgc tgagaaagca ggcgtctcca
     2161 ggcaaacgat catcgccgta gaagcaggga agtactcgcc cacacttgaa ctggctttcc
     2221 gcatcgccga cgcattcggc ctgccgatca ctgaggtgtt cgggtacaag accaagggga
     2281 gtacgtgatg aggaccggta acgagagaag aaggaatcag aacatgagga atgtgcaatg
     2341 aaagcggtaa tctatgacaa gaagtctcac gatcaccttc gctgccgtga cgttgaaaag
     2401 cctgtgcccg gcgacggaga agt
//
LOCUS       DATMZD010000002         3769 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_10140,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000002 DATMZD010000000
VERSION     DATMZD010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3769
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10140"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VN478_00025"
     CDS             <1..249
                     /locus_tag="VN478_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21101.1"
                     /translation="ALERPQADLPSIRPALIGLPRLDAVIVRQQAIASLPVNTQASVL
                     TRLVDRGTSIFVTDTPRHIAELLDDAGHDPWNSSPTQK"
     gene            222..935
                     /locus_tag="VN478_00030"
     CDS             222..935
                     /locus_tag="VN478_00030"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HWQ21102.1"
                     /translation="MEFLTYSEVASTQETARILAAEGRALPFAVVAETQSRGRGRRGK
                     NWDSPRGGLWLTLALAVPSAGAVHQSAIVAAHAVATVVLTATGLHTLVKWPNDLLVER
                     RKVCGILAETLVQTDTTLLMVGIGLNVNNVVPPDQLPQATSLKAELEHDLDVWQLATS
                     VVTMVQLDIGTLITAGFDPFTPWIETSLALREELVTVEFNHQRTSGRVAGVSRTGALL
                     LKNPNGSLEEVDAGSVYNW"
     gene            complement(951..1802)
                     /locus_tag="VN478_00035"
     CDS             complement(951..1802)
                     /locus_tag="VN478_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HWQ21103.1"
                     /translation="MGSIMEASLFTRKIEAEIIKEVERLKSEGHNPSLSVIVVGNSPS
                     TMVYFKNMSHKGVELGFYVELRKIDESIGAAEVLELIHKLNDSKDVDGILVGLPLPKE
                     YDVDSILHSISPDKDVDAFHPFNMGQLMIGKPRFVPATARSIISLLENYGVRIQGKNA
                     VVVGRSNIVGKPVASLLLQRDATVTVCHSKTVDLVKYTRDADILIVSMGRPRAITADY
                     VKEGAVVIDVGINDVNGKIVGDVDFDGVRPKASLITPVPGGVGVLTTLMLFDNVLKAA
                     RLNSDKH"
     gene            complement(2091..3272)
                     /locus_tag="VN478_00040"
     CDS             complement(2091..3272)
                     /locus_tag="VN478_00040"
                     /inference="COORDINATES: protein motif:HMM:NF019585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HWQ21104.1"
                     /translation="MQKGYWEDAGKLQFDGVVASTEDTARGLVVRLEKTYFYPESGGQ
                     LADGGTVDGTAILDARQDASGPFVVLPSGFFAGVGQHLACVVDAEKRGRHTQLHSAQH
                     ILSRLLDDRGIRTLSFHMTEDEASIEIETTTLSSSVQSEIEDAVERLVWRCLPVDTLF
                     VDAAHIGSYDVRKVPELAGGPLRLVRIGDIDTNPCGGTHVASTGEIGGFAIVRTDKVR
                     GNTRLYFVAGRTATAYQRRNEVVLRDIEQSLTCGLGDVVASIARLRQHDHEVSAHARG
                     LQAIAVDELARQAMDDLTSHGTAAMVLDSVPAELARMIVARLPSAPGPLCVLIYPAGG
                     VEGQFVCSVPPGSEPLLAAFNARMKETFGARSGGAGRVIQGKIDARLTSDQLKSLLVA
                     G"
     gene            complement(3290..>3769)
                     /locus_tag="VN478_00045"
     CDS             complement(3290..>3769)
                     /locus_tag="VN478_00045"
                     /inference="COORDINATES: protein motif:HMM:NF023428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HWQ21105.1"
                     /translation="ALYYMARMIDAGDDPLFVARRMIIFAAEDVGLANPFAVVVATAA
                     YDAVHAVGLPEAMLNLTEAVIYLAGTRKNNSVLGAMRRARDLAQQTKNVDTPVQLKNS
                     HAGQALYKYPHNFPGSWVAQEYFPDEVHDQAIYLPTGAGYERVIVGYLKSLRGKKPE"
BASE COUNT          786 a         1203 c         1023 g          757 t
ORIGIN      
        1 gcactggaac gccctcaggc ggaccttcca tccatacgac cggcactgat cggtctccct
       61 cgactggatg cggtcattgt gcgacagcaa gccatcgcct cacttcccgt gaacacgcag
      121 gcaagtgtgc tgacccgtct cgtcgatcgc gggacaagca tattcgttac agatacacct
      181 cgccacatcg ctgaactcct cgacgatgca ggtcacgatc cgtggaattc ctcacctact
      241 cagaagtagc gtcgacgcag gaaacagccc ggattctcgc agccgaaggc cgtgcactgc
      301 cattcgctgt tgtggccgag acccagtcgc ggggcagagg acgtcgcgga aagaactggg
      361 attcgcctcg aggaggcctg tggcttacgc tcgccttggc cgtgccgtca gccggggctg
      421 ttcaccagtc cgcgattgta gcggcacatg cggtagcgac cgtcgtgttg accgcgaccg
      481 gccttcacac gctcgtcaag tggcccaacg atctccttgt cgagcgccga aaggtctgcg
      541 gtatccttgc cgagacgctc gtccagaccg acacgacgct cctcatggtc ggcataggcc
      601 tgaacgtcaa caatgtcgta ccgcccgacc agctgccaca ggcgacttct ctgaaagcgg
      661 agctggaaca cgatcttgac gtatggcaac tggcgacgag tgtcgtcacg atggtgcaat
      721 tggacatcgg tacactcatc actgctggtt ttgacccatt caccccctgg atcgaaacca
      781 gtcttgcgtt acgggaggag ctcgtcaccg tagagttcaa ccatcagagg acatccggtc
      841 gggtagccgg agtgtcaaga accggcgccc tgcttctcaa gaaccctaac ggatcgctcg
      901 aggaggtgga cgccggaagc gtctacaact ggtgaccggt cttcgccggc tcagtgtttg
      961 tccgagttga gtcgggcggc cttcaggacg ttgtcgaaca gcatgagggt cgtcagcaca
     1021 ccgactccgc cgggcacggg agtaatcaac gaagcctttg gacgaacacc gtcaaagtcg
     1081 acgtcgccca cgatcttccc gttgacgtca ttgattccaa cgtcaatgac gacggcaccc
     1141 tccttgacgt agtcggccgt aatcgcccta ggcctcccca tcgagacgat caggatatcg
     1201 gcatcgcgcg tgtacttgac cagatcaacg gtcttggagt ggcacaccgt gacagtagca
     1261 tcgcgctgca gcaacagaga cgccaccggc ttgcccacaa tgttgctgcg accaacaacg
     1321 accgcgttct tgccctgaat gcggacaccg tagttctcca ggagcgaaat gatcgaccgc
     1381 gccgtagcag ggacgaaacg aggctttccg atcatcaact gccccatatt gaacggatgg
     1441 aaagcgtcga cgtccttgtc aggactgatt gagtgcagga tcgaatccac gtcgtattcc
     1501 ttgggcaacg gaagcccgac gagtatgccg tcgacgtcct ttgagtcatt cagtttgtgg
     1561 atgagctcaa gaacctcggc agctccgatc gactcgtcga tctttcgaag ctcaacatag
     1621 aaccccagct ccacgccctt gtgcgacatg ttcttgaagt agaccatcgt ggagggactg
     1681 ttgccaacca caatcacgct caaagatgga ttatgccctt ctgacttcag acgctcgact
     1741 tccttaatga tctcggcctc gatcttgcgc gtaaacagcg atgcttccat aatggatccc
     1801 atgtctctct ccttttcgac gcgacctgtc ggctattcga tcggtccaga accgtagcaa
     1861 tcgccagcca tgcccacctg gtgccgctcg caagggcggg tcttgtgacc cccgcttgcc
     1921 actgttcgta tccctctggc ctccagaaga agatactgtc agaggcagat tctcgccccg
     1981 caacgaagac catacgtcat gcaccacatc atcactggac ccacctccct taccagtgta
     2041 gttggaacgc cagtgcaatc aacagaacac ggcttgctga ccgtcactgc tcatcctgca
     2101 acgagaagag acttcagttg atccgacgtc agacgtgcgt cgatcttccc ctggatgact
     2161 cgtcctgcgc caccggatct cgcgccaaag gtctccttca tccgtgcatt gaaggctgcc
     2221 agcaacggtt cgcttccagg cggaaccgaa cagacgaact gcccttcgac accgccggct
     2281 gggtagatga ggacacatag cgggcctggg gcggatggca accttgcgac gatcatgcgg
     2341 gcgagttcgg cgggaaccga gtcaagaacc atggcagcag taccgtgcga cgtgagatca
     2401 tccatagcct gacgcgcaag ttcgtccacg gcaatcgcct gcagacctct ggcatgcgct
     2461 gagacctcgt ggtcgtgctg acgcagcctc gcaatcgagg ccacgacatc accaagtcca
     2521 cacgtcagcg actgttcgat atcccgaagg acaacctcat ttcgccgctg atacgccgtc
     2581 gccgtcctcc cggctacgaa atacagccga gtgttgcccc gcaccttgtc cgttcggaca
     2641 atagcgaatc cgccaatctc gccggtcgat gcgacgtgtg tcccgccaca tggatttgtg
     2701 tcgatgtccc cgatccggac gaggcggaga ggtccccctg caagttcggg caccttgcgc
     2761 acatcgtagc tgccaatgtg ggccgcatcc acgaacagcg tgtcgactgg aaggcacctc
     2821 cacacaaggc gctccacagc gtcttcgatc tcggactgga cactgctgct cagagtcgtg
     2881 gtctcaatct cgatactggc ctcatcttcc gtcatgtgga aggacagggt ccgaatgccc
     2941 ctgtcgtcca gcagtctgct gagaatgtgc tgagctgagt gcaactgggt atggcgaccc
     3001 ctcttctcgg catcaacaac acaagccagg tgctgaccga cccccgcgaa gaacccggag
     3061 ggcagtacga caaatggtcc cgaagcatcc tgccgtgcgt cgagtatcgc cgttccgtcg
     3121 accgtcccac catcagcgag ctgtccacca ctctcgggat agaagtaggt cttctcgagc
     3181 cgcacgacaa gacctcgcgc tgtgtcctcg gtcgatgcca cgacgccgtc gaattgaagc
     3241 ttgccggcgt cttcccagta ccccttctgc atttcggact ccttcgctcc tattccggtt
     3301 tcttgccgcg aagcgacttc agatacccta cgatcacacg ctcatacccc gctccggtgg
     3361 gcaggtagat ggcttggtca tgcacttcat ccggaaagta ctcctgggcc acccagctgc
     3421 caggaaagtt gtggggatac ttgtacaacg cttgcccagc atggctgttc ttgagctgaa
     3481 ccggcgtatc gacgttcttc gtctgctgag ccaggtcacg cgctcgacgc atggcgccca
     3541 gaaccgagtt gttcttccgc gtcccagcca ggtagatgac agcctccgtc agattgagca
     3601 tcgcctcggg cagccccact gcgtgtaccg cgtcatatgc cgccgtcgcg actaccaccg
     3661 caaacgggtt cgccaggccg acgtcctctg cagcgaagat gatcatccgg cgagcaacga
     3721 aaagggggtc gtccccggcg tcgatcatac gggccatgta atacagagc
//
LOCUS       DATMZD010000003         2118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_100743,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000003 DATMZD010000000
VERSION     DATMZD010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100743"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1019)
                     /locus_tag="VN478_00050"
     CDS             complement(<1..1019)
                     /locus_tag="VN478_00050"
                     /inference="COORDINATES: protein motif:HMM:NF014851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HWQ21106.1"
                     /translation="MILDLAGTWKVSDDEGQYSFVGNVPGTVQGDLVRQGLVPHPYVG
                     INEQAMRDLENKSWTCVTEFEVDDVPAEENVELVFEGVDTLVDVHLNGQYLGSTEDMF
                     LEYRFAVKNVLKKGKNVLQVHIKSPVAGPAALEQEYGRLGAVEESVRAYIRKAQYSYG
                     WDWGIRLPTSGIWRPVYVESYDRARLTGCSAVLEQQDDGAGTVRVLGSVVPGVDSGEA
                     ANYTVAVRVDDALVGQFPIRHVGEELAFDGVFSLQHARLWYPNGLGDHYLYAFEFTLK
                     ADGVPVCTRKEKIGLRTVTLARETDAGGESFIFTVNGKRVFAKGANWIPADGILSWIQ
                     PDDYS"
     gene            complement(1057..2001)
                     /locus_tag="VN478_00055"
     CDS             complement(1057..2001)
                     /locus_tag="VN478_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWQ21107.1"
                     /translation="MLRLDGVSKVYKVGTFGGRKLLAVSNVSFDVQDGEVTSLIGESG
                     SGKTTIGKMILHLASMSAGTIKLDGVDVSKLKGNALKEYYRKVQGVFQDPFSSYNPIF
                     KADRIFRTIREEFFPGIGNGEWNTKIDGSLRAVGLNPDEVVNKFPHQLSGGQLQRFLI
                     ARALLLDVKFLVADEIISMLDASTRVGVLNLLADLKCRGLSILFITHDLSLGYYISEK
                     SVILYRGRVVEMGPTEHVYHHPLHPYTKMLMACVPRVGEKWACTDVPETILTTEHSGL
                     SGGCAYYDRCTLDGKNGECNTSPSLVEVEPGHFVACWH"
     gene            complement(2007..>2118)
                     /locus_tag="VN478_00060"
     CDS             complement(2007..>2118)
                     /locus_tag="VN478_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014913533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWQ21108.1"
                     /translation="CRFRERCPLAFGKCLEVPPFVEVGENHFAACWKVGA"
BASE COUNT          417 a          687 c          555 g          459 t
ORIGIN      
        1 ctgctgtagt cgtccggttg tatccagctg aggatgccgt cggctggtat ccagttcgct
       61 cccttcgcaa agacgcgctt gccattgaca gtgaagatga aggactcgcc cccggcatcc
      121 gtttctctcg ccagcgtgac ggtgcgcagg ccgatcttct ccttcctggt gcagacgggg
      181 acgccatcgg ccttcaacgt gaattcaaac gcgtacagat agtggtcacc caggccattc
      241 gggtaccaca ggcgggcatg ctgcagcgag aatacgccgt caaacgcgag ttcctccccg
      301 acatgcctga tgggaaactg accgacgagt gcgtcatcaa cccgtaccgc caccgtgtag
      361 ttcgcggctt caccagaatc gacgcccggc accacggagc ccaggaccct taccgttcct
      421 gcgccatcgt cctgctgttc caggaccgcg gaacatcccg tcaagcgtgc cctgtcatag
      481 gactccacat agacgggcct ccagatgccg gacgtcggca gacgaatacc ccagtcccag
      541 ccgtaggagt actgtgcctt tctgatatag gccctgacgg attcctctac ggcaccgagt
      601 ctgccgtatt cctgctcaag tgctgccggt cccgctaccg gggacttgat gtgaacctgc
      661 aggacgttct tgcccttctt gagcacgttt ttgacggcaa acctgtactc cagaaacatg
      721 tcctctgtgc ttcccaggta ctgtccattg aggtgaacgt ccaccagtgt gtcgacaccc
      781 tcaaacacca gctcgacatt ctcttcggcc ggcacatcgt ccacttcgaa ctcggtgacg
      841 caggtccagg acttgttctc gagatcccgc atggcctgct cattgatgcc cacgtacggg
      901 tgcggcacca gtccctgtcg gacgaggtcg ccctggaccg ttccgggcac gttcccgacg
      961 aaggaatact ggccttcgtc atcactgacc ttccatgttc ctgccagatc gaggatcacg
     1021 agtattgccc ctctgctgtc gcagtctccc cttcctctag tgccagcagg cgacaaagtg
     1081 gccgggttcg acctccacca acgacggcga cgtgttgcat tctccgttct tgccgtccag
     1141 agtgcacctg tcataatacg cacagccacc ggagagccct gaatgctctg ttgtcaggat
     1201 cgtctccggg acgtccgtgc acgcccactt ttcacccacc cgtggaacgc atgccatgag
     1261 catcttcgtg tacggatgaa gcggatgatg gtagacgtgc tctgtcggac ccatctccac
     1321 gacacgcccc ctgtacagga tcacggactt ctcgcttatg tagtagccca gtgagaggtc
     1381 gtgtgtaatg aagaggatgg acagcccacg acacttgagg tctgcgagca ggttgagaac
     1441 gcccacgcgc gtcgacgcat cgagcatgct gatgatctcg tctgccacaa ggaacttgac
     1501 gtcaagcagc agagcgcgcg caatgagaaa ccgctgcagc tgccctccgc tcagctggtg
     1561 gggaaacttg ttgacgacct cgtcgggatt gaggcctacg gccctcaacg agccgtctat
     1621 cttggtgttc cattcgccgt tgccgattcc agggaagaac tcctctctga tcgttctgaa
     1681 gatccgatcg gccttgaaga tcgggttgta ggaactgaat gggtcctgaa agacaccctg
     1741 gaccttccga tagtactcct tgagtgcgtt ccctttgagc ttggaaacgt cgaccccgtc
     1801 cagcttgatc gtacccgcac tcatcgaggc gagatgcagg atcatcttcc ctatggtggt
     1861 cttcccgctt ccgctctcac caatcagcga cgtaacctcg ccatcctgca cgtcgaagct
     1921 gacattgctc actgcgagga gcttcctccc cccgaacgtc ccgaccttgt agaccttcga
     1981 tactccgtca agtctgagca tttccctcag gcacctacct tccagcacgc agcgaagtga
     2041 ttctcgccga cttcgacgaa aggcggcacc tcgaggcatt tcccaaaggc cagagggcac
     2101 ctttccctga accggcat
//
LOCUS       DATMZD010000004         2030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_60513,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000004 DATMZD010000000
VERSION     DATMZD010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60513"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1017)
                     /locus_tag="VN478_00065"
     CDS             complement(<1..1017)
                     /locus_tag="VN478_00065"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWQ21109.1"
                     /translation="MNYRTFGKLDWKPSALGFGTMRLPIVGEDPSAIDEHQAIAMLRM
                     GIDGGINYVDTAYPYHGGKSETLVGKALQEGYRDKVHLATKMPSWLIESQTDMTARFE
                     EQLQRMQTDHVEFYLLHALNAARWHKLRDAGVLEWAHERIREGRIGHLGFSFHDSNAV
                     FHEIVDAYQDWAFCQIQYNFLDEQTQAGTEGLQYAASKGMGIVIMEPLRGGKLAAPNT
                     GISAMWLAASTERSAAEWALDWVWNHPEVSLLLSGMSTPEQVQQNLDAASRSEAGMLA
                     PGDLDLIARVRDAYDALIRVPCTGCRYCVPCPQGVDIPGMFELVNEGSMFGNMDTQRK
                     RYVHA"
     gene            1247..>2030
                     /locus_tag="VN478_00070"
     CDS             1247..>2030
                     /locus_tag="VN478_00070"
                     /inference="COORDINATES: protein motif:HMM:NF013942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HWQ21110.1"
                     /translation="MKKRVAVVFGSRSVEHEVSIITAVQAMNSLDEEQFEVVPLYVSK
                     EGVWFTGDALKTMDVFRDLVSLPGKAQRVHLQPFAGKSTLAVQVENKGFMGRGMDVKP
                     LAVDVALLCNHGTNGEDGTMQGMFELLGIPYTSAGVTGSSLGMDKVFQKQVYAAAGIP
                     VVPYVSFGRRAWRASPDDCLRLAEQRLKFPMFVKPANLGSSVGITRATDHDTLRYALE
                     VASHFDRKLLVEQAVVNHREVNCAVLGNDEPIPSACEEVFSSH"
BASE COUNT          372 a          580 c          637 g          441 t
ORIGIN      
        1 cgcatggacg tatctcttgc gctgggtgtc catgtttccg aacatcgacc cctcgttgac
       61 gagttcgaac atgccgggga tatcgacccc ctggggacat ggcacgcagt agcggcatcc
      121 cgtacaggga acacggatga gagcgtcgta ggcgtcccgg acgcgcgcga tcaggtccag
      181 gtcgccagga gccagcatgc cggcttctga cctgctggcg gcgtccagat tctgctgaac
      241 ctgctccggc gtgctcatac cactgagtag tagcgacact tcggggtggt tccacaccca
      301 gtccagtgcc cattcagcgg ccgaccgctc ggtcgaggcc gcaagccaca ttgctgagat
      361 acctgtgttg ggcgccgcca gtttgcctcc ccgaagaggt tccatgatga cgatccccat
      421 acccttggat gcagcatatt gcagtccttc ggtaccggcc tgtgtctgtt cgtcgagaaa
      481 gttgtattgg atctgacaga aggcccaatc ctggtatgcg tcgacgattt cgtggaagac
      541 ggcgttgctg tcatggaagg agaagcccag atgacctatg cgcccctccc gaatgcgttc
      601 gtgcgcccac tcgaggactc cagcatccct gagcttgtgc cagcgggcgg cgttgagcgc
      661 gtgcagcagg tagaactcga cgtggtcggt ctgcatgcgc tgcagctgtt cttcgaaccg
      721 tgctgtcatg tcggtctgtg attcgatgag ccacgagggc atcttggtgg ccaggtgcac
      781 cttgtcccgg tatccctcct gcagggcctt gccgacgagc gtctcactct tgccgccgtg
      841 gtagggatag gccgtatcga cgtagttgat ccctccatcg atgcccatgc ggagcatggc
      901 gattgcctgg tgctcgtcga tggccgaggg atcttccccg acaatcggta gcctcatcgt
      961 gccaaatccg agtgcagacg gtttccagtc gagcttcccg aacgttctgt agttcacgtg
     1021 gcacatcctt tcgcagagaa tcgttcgtat tccaatatat aacgccgagc gtccctgtca
     1081 acgcgatccg gttgtgtgcg gccggatgag tgcacctgga gcgcgttctc tcgttgcaca
     1141 agttgcaaag gcggccaaaa ggggatagac tgaacgcgag caaaatcggt agttcacggg
     1201 ctgttgaggg gacgtctgcc ttgcgggccg atgtgaggag ccattgatga agaagagggt
     1261 cgccgttgtc ttcggctcgc gctcagtgga gcacgaggtc tctatcatca ccgccgttca
     1321 ggcgatgaat tcgttggacg aggagcagtt cgaggtcgtc ccgctgtacg tgtccaagga
     1381 aggtgtctgg ttcacgggag atgccctgaa gaccatggac gtgttccgtg acctggtcag
     1441 ccttccgggc aaggcgcagc gcgtgcacct gcagcccttt gctggaaagt cgaccctggc
     1501 tgtgcaggtc gagaacaagg gcttcatggg tcgtggcatg gatgtgaagc cgctggctgt
     1561 cgacgtcgca ttgctgtgca atcatggcac caacggcgaa gacggtacca tgcagggcat
     1621 gttcgaactc ctggggatac cctacactag tgcgggcgtg accggttcct cactgggcat
     1681 ggacaaggtg ttccagaagc aggtgtatgc cgccgcgggg atccccgtcg ttccatatgt
     1741 cagtttcggc cggcgtgcat ggcgtgcttc gccggatgac tgtcttcgtc tggcagagca
     1801 gcgactcaag tttcccatgt tcgtgaagcc agccaacctg ggctccagcg ttggcatcac
     1861 gcgcgcgacg gatcatgaca cgctgcgcta tgcacttgag gtcgcaagcc attttgaccg
     1921 gaagctgctg gtggagcagg ccgtcgtcaa tcatcgcgag gtcaactgtg ccgttttggg
     1981 caacgacgag ccgataccct cggcatgtga ggaggtcttc agctcgcacg
//
LOCUS       DATMZD010000005         2734 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_20290,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000005 DATMZD010000000
VERSION     DATMZD010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20290"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="VN478_00075"
     CDS             <1..526
                     /locus_tag="VN478_00075"
                     /inference="COORDINATES: protein motif:HMM:NF021610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2344 domain-containing protein"
                     /protein_id="HWQ21111.1"
                     /translation="ETWSPIADRAKALERTSPPGLRFLAAADVPLTAPSLMGQPMVLR
                     YEYAWLHRADVDADRMKAHIASFLALTTLMVERENSKSRAPKDIRKYVLTAQISTRTP
                     LRAHVGVFFDQSGTIRMDEVINHIFGLDASVGPMPIITRQSVLLDVNGTLVDPMDVGA
                     QTLPAHKSKPAHRD"
     gene            complement(593..2419)
                     /gene="typA"
                     /locus_tag="VN478_00080"
     CDS             complement(593..2419)
                     /gene="typA"
                     /locus_tag="VN478_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007390413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HWQ21112.1"
                     /translation="MHRNDLRNIAIIAHVDHGKTTLVDAMMRQCKLFRNIDQMGNCIL
                     DSGDLERERGITIISKNMSLVWKGVKINIVDTPGHSDFGGEVERVLRMVDGVLLLVDS
                     SEGPMPQTRFVLKKALELNLRPIVVINKIDRQDARIQEVINETFDLFVELDASEEQLD
                     FPVVYASGRDGKATMDLNDPPQDLTPLFQLMLKKVPAPEYDPGSPLSVLVTNITHDDY
                     IGRIGIGRMFGGQLKAGDKVKVFSGDAEYMGQILKLFVFEALERKEVESVEAGEIFAF
                     AGIPAITIGDSVTDPERPVKLNMLTIEPPTVSMMFMVNDSPFGGTEGKFVTSNKLRER
                     IEKELRTNVSIAVDWGDLPDRFTVAGRGELQLAILIEQMRREGYEFQVSMPQVITEKE
                     SGKVLEPYETLVLDVPKEYQGTVINMLGERRGTMEKMLDLKGAYVRLSYTIPLRGLFG
                     FRSEFLSSTKGNGVMTHTYLGYLEWAGKIGGRRNGVLVSDHTGAVSAYSIARIQERGK
                     LFVAPAEKVYAGQIVGETQRDHDLKVNPTKEKHLSNMRSSTSEISTKIDPPVQFALDQ
                     FLDYINEDELLEVTPKSLRIRKRLLDFKERKRSGREEDDYGD"
     gene            complement(2492..>2734)
                     /locus_tag="VN478_00085"
     CDS             complement(2492..>2734)
                     /locus_tag="VN478_00085"
                     /inference="COORDINATES: protein motif:HMM:NF013665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HWQ21113.1"
                     /translation="ALFPDACQVKGFTGRIQGDADVIIVPNIESGNVFYKVLCRFGGG
                     DVAAMVTGTTAPCILTSRGDSEDSKFYSIALGALNA"
BASE COUNT          527 a          854 c          766 g          587 t
ORIGIN      
        1 cgaaacctgg tcgccaattg ccgatcgcgc gaaggcactc gagcgtacct cccctcccgg
       61 cctcagattc ctggcagcgg cagatgtccc cctcaccgcg ccctccctca tgggtcagcc
      121 catggtgctg cgatacgaat acgcgtggct gcacagggca gacgtggacg ctgaccggat
      181 gaaggctcac atagcttcct tcctcgccct gacgaccttg atggtggagc gcgagaacag
      241 caagtcccga gctcccaagg acattcgcaa atacgtcctt acggcgcaga tctcgacgcg
      301 gacgcctctg cgcgctcacg tgggagtgtt cttcgaccag tcgggcacaa tccgcatgga
      361 tgaggtcatc aaccatatct tcgggctcga cgccagtgtc ggtcctatgc ccatcatcac
      421 tcgtcagagc gtcctgcttg acgtgaacgg cacgctcgtc gaccccatgg acgtcggggc
      481 gcagaccctt cccgctcaca agtcaaagcc cgctcaccga gactgagact gcttaacgac
      541 agactccccg gttgtccggg gagtcggggg ttcgtcacaa tctcggcgtg agctagtcgc
      601 cgtagtcgtc ctcttcgcgt cccgaacgct tccgctcctt gaaatcgagg agtctcttgc
      661 ggatgcgcaa tgacttgggc gtgacctcaa gcaactcgtc ctcgttgatg tagtccagaa
      721 actggtccaa ggcaaactgg acgggcgggt cgatcttcgt ggagatctcc gacgtggatg
      781 accgcatgtt gctgaggtgc ttttccttgg tcggattcac cttcaggtcg tggtcgcgct
      841 gggtctctcc gacaatttga cctgcataca ctttttcggc cggagcgaca aacagcttgc
      901 cccgctcctg gatgcgcgcg atggaatacg ccgacaccgc tccagtatgg tcggagacca
      961 ggaccccatt cctgcggccg ccgatcttgc cggcccactc gaggtacccg aggtaggtgt
     1021 gtgtcatgac gccattcccc tttgtcgagc tcaggaactc agaccggaag ccaaacaggc
     1081 cccgaagggg gatcgtatag ctgaggcgca cataggcccc cttgaggtca agcatcttct
     1141 ccatggtccc gcgccgctct ccgagcatgt tgatgaccgt gccctggtat tccttgggaa
     1201 cgtcgagcac cagcgtctca tacggctcaa ggaccttgcc gctctctttc tccgtgatga
     1261 cctgaggcat cgatacctgg aactcatatc cttcgcggcg catctgttcg atgaggatcg
     1321 ccagctgaag ttcgcctctc cccgcgacgg tgaaccggtc gggcagatca ccccaatcga
     1381 cggcgatgga cacgtttgtg cgcagttcct tctcgatgcg ctcacgcagc ttgttggagg
     1441 tgacgaactt gccctccgtc ccaccgaacg ggctgtcgtt gaccatgaac atcatcgaca
     1501 ccgtgggggg ctcgatggtc agcatgttca gcttcaccgg acgctccggg tcggtgaccg
     1561 agtccccgat cgtgatggca ggtatgcccg cgaatgcaaa gatctcgccg gcctcgacgc
     1621 tctcgacttc cttgcgctcc agagcttcga agacgaatag cttgaggatc tgccccatgt
     1681 actcggcgtc accagagaac accttcacct tgtctcccgc tttcagctgt cctccgaaca
     1741 tgcggccgat gccgatgcgg ccgatatagt cgtcgtgtgt gatgttcgta accagcacag
     1801 aaagcggact cccggggtcg tactcggggg ccgggacctt cttgagcatg agctggaaga
     1861 gcggcgtcag gtcctgtggc ggatcgttga gatccatggt ggcctttccg tcgcgacccg
     1921 aagcgtagac aaccgggaaa tccagctgct cctcacttgc atcaagctcc acgaacaggt
     1981 cgaaggtctc gttgatcact tcctggatac gggcatcctg gcggtcgatc ttgttgatga
     2041 cgacgatcgg cctgagattg agctcaagag ccttcttcaa cacgaacctc gtctgaggca
     2101 tgggtccctc ggacgaatcg accagcagga gaacgccgtc gaccatcctc aggacgcgtt
     2161 ccacttcacc gccgaagtcc gagtgaccgg gggtatcgac aatgttgatc ttgacgcctt
     2221 tccacacgag cgacatgttc ttggagatga tggtgatacc tctctcccgt tcgagatcac
     2281 ccgagtcgag gatgcagttg cccatctggt cgatattgcg gaacagcttg cactgccgca
     2341 tcatcgcgtc gaccagcgtc gtcttcccat ggtcgacgtg cgcgataata gcgatgttgc
     2401 gaagatcatt tctatgcact gaaacactcc ctttcaatca agcgtggctg acggccgaca
     2461 atggcctcaa actattatta tacgcttgtt ctcaggcgtt caatgccccg agtgcgatgg
     2521 aatagaactt ggagtcttcc gagtcgcccc tgctggtgag gatgcaggga gccgtcgtcc
     2581 ccgttaccat agccgcgaca tcgcccccgc caaacctgca tagcaccttg tagaacacgt
     2641 ttccgctctc gatgtttggt acgatgatga cgtcggcgtc cccctggatg cgaccggtga
     2701 agcccttgac ctgacacgca tcggggaaca gggc
//
LOCUS       DATMZD010000006         5050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_70720,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000006 DATMZD010000000
VERSION     DATMZD010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5050
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70720"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..538
                     /locus_tag="VN478_00090"
     CDS             155..538
                     /locus_tag="VN478_00090"
                     /inference="COORDINATES: protein motif:HMM:NF018644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HWQ21114.1"
                     /translation="MTAVVHARRHIVATAMILTMGPAVGVMFLLHSLLPALDWLQCWL
                     LSITGITFLVYGYDKLIAGTQATRVPEKVLLTLAFTGGTLGAIAGMRFFHHKTSKESF
                     LERFWLIVVAQIVVIAGWYVFLRPR"
     gene            853..1353
                     /locus_tag="VN478_00095"
     CDS             853..1353
                     /locus_tag="VN478_00095"
                     /inference="COORDINATES: protein motif:HMM:NF016413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HWQ21115.1"
                     /translation="MRLIIKQKVFSWADRFRVWDEDNRDRYFVEGEFFSFGKRLHIMD
                     PDDREVALVQQKVFSLLPRFFVVVDGVQVAEIVKEFTFFHPRYTIGGLGWDIDGDFFA
                     HDYSISQDGREIVTIHKEWLTWGDCYVLDIADPKDTVTALAVVLAIDCVQAAAAAAGA
                     ASNTGH"
     gene            complement(1417..1839)
                     /locus_tag="VN478_00100"
     CDS             complement(1417..1839)
                     /locus_tag="VN478_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HWQ21116.1"
                     /translation="MNAAELVDGLIASLGDWRGAALASIRRVIHEADPDVVEEWKWMG
                     SPAWSHDGMICLANAFKDKVKITFPEGASLADPDKLFNNGLAGKQWRSIDISEGDTIN
                     ERSLKALVREVIDHNHAKGPSASLPHAARANPKKKPEK"
     gene            2146..2526
                     /locus_tag="VN478_00105"
     CDS             2146..2526
                     /locus_tag="VN478_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWQ21117.1"
                     /translation="MERTRLISELWNAMDRRQWEELAAYFCDDAVVFWPSTNERFTVP
                     EFIEVNRAYPGTWTIQIERLQQVDDLVVSVVKVALAEGRTSFHAVSFFEFRGDKIGTL
                     YEYWGEDGDPPQWRINRSIGTRIS"
     gene            2834..3436
                     /locus_tag="VN478_00110"
     CDS             2834..3436
                     /locus_tag="VN478_00110"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWQ21118.1"
                     /translation="MKQEERSQETRSRILAVAEQSFADHGYDVTSVDSICRAAGVSKG
                     AFYHHFPSKGSVFVALLNAWLGDLDRQFAAAQAGTESVPQQVATMAAGVNEIYHIAGT
                     KWNILLEFWAKSKADPEVAASTTDMLHRYQGFFQEMLERGRSEGSLKVKDSSLAATVA
                     LSVVLGLLLQGILDPESQDWGALMQRALAMLMGSMSGRNT"
     gene            3433..4419
                     /locus_tag="VN478_00115"
     CDS             3433..4419
                     /locus_tag="VN478_00115"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWQ21119.1"
                     /translation="MRVLVTGAFGNVGTYAIDELLSRGASVRCLDIPSPMSKRKARPY
                     AGKIEILWGDIRNTDVVARAVKGVDAIVHLAFIIPPRSEQRPAWAETINVGGTWNLLQ
                     AALASKSRPRIVFSSSIALYGRTQHLAPPRKADELLHPYEDYAYHKMTCEHMLHASGL
                     PWVILRFGAVPPIEFGELDPLMFEVRMDDRMEYLAPMDAGAAVAAAALLPGLEGRTLM
                     VAGGPSCQVTGREFMQRSFDALGIGMLPESAFSQIPFHCDFMDTEESERLLHYQHHSF
                     DETLALQRKTLGWKKLFVPIARPFIRRKLLAMSPYYRRKMTGSVPLPQTDQT"
     gene            4867..>5050
                     /locus_tag="VN478_00120"
     CDS             4867..>5050
                     /locus_tag="VN478_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21120.1"
                     /translation="MSDPVREYYFIVPDAQDAHVLVVPEGGVWTLPHTSIPGVHKWQS
                     VAHVNKAANEALGFRTT"
BASE COUNT          999 a         1501 c         1523 g         1027 t
ORIGIN      
        1 gcgacatgga cataacattg gagtacaggc caggcgacgg tgccgccgcg ggcatgacgg
       61 aagccggcgt ccacatcatc cagaagccgt ttgccgtggg cagtccggcc gcggaggtcc
      121 gggacatacc ggacgtgaac aggagtgtat ccccatgacc gcggtcgttc atgcacgccg
      181 tcacattgtg gccacagcga tgatcctcac gatgggccca gcggtcgggg tcatgttcct
      241 tctgcacagc ctccttcctg cgctcgactg gctgcagtgc tggcttctgt ccatcactgg
      301 tatcacattt ctcgtctacg gctatgacaa gctgattgca gggacccagg cgacccgcgt
      361 gcccgagaag gtcctcctga cccttgcatt cactggcggc acgctcggag cgattgcggg
      421 tatgagattc ttccaccaca agaccagcaa ggagagcttc ctggaacggt tctggctgat
      481 cgtcgtcgcg cagatcgtcg tcatcgccgg ctggtatgtc ttcctgcggc ctcggtgaac
      541 cctgtccagc aggaaacgac gtcgtctgcc gatgacgtct ccaccatgag gcttcagtga
      601 ttgacgccga agtgcccctg gcgggatagt cttgtggggt cgtctgacgc cagtgcctct
      661 cgtgtcgtac agcaccgccg gcacggaccc cgctgccatt gtttgagcac acctccagac
      721 tagccagaac actgctattg cggtacactt gttatggagg gcgtgattct gagtgtgatg
      781 tcatgcggat gtggcacgcc gactgctccg tgactggaac aaatactgca ttaaacctgc
      841 gaggtgttcg aaatgcgtct gattatcaag cagaaggtct tttcctgggc agacagattc
      901 cgcgtctggg atgaggacaa ccgtgaccgc tacttcgtgg aaggagagtt cttctcgttc
      961 gggaagagac ttcatatcat ggatccggac gaccgggagg tcgctcttgt ccagcagaaa
     1021 gtcttctcct tgctgccccg gttctttgtc gtggtggacg gcgttcaagt ggcggagatc
     1081 gtcaaggagt tcaccttctt ccatccccgc tacaccatcg gcgggttggg gtgggacatc
     1141 gatggtgact tcttcgccca cgactactcc atctcccagg acgggcgcga gatcgtcacg
     1201 atccataagg agtggctgac ctggggcgac tgctacgtgc tcgacatcgc cgatcccaaa
     1261 gacaccgtta cggcgctggc ggtagtgctg gccatcgatt gcgtccaggc cgcagcggca
     1321 gcggcgggag ctgcgtcaaa cactggccac tgacagaccc atccagcaca gtccgtctgc
     1381 accagtactc cagcacagtg tgccgtgctg gagtatctac ttctccggct ttttcttcgg
     1441 gttggctcgt gccgcgtgcg gcaggctcgc cgatggcccc ttggcatggt tgtggtctat
     1501 gacctcgcgg acgagggcct tcagcgatcg ctcgttgatg gtgtcgcctt cgctaatgtc
     1561 gatgctcctc cattgcttgc ctgcgagtcc gttgttgaag agcttgtcgg gatccgccag
     1621 gcttgctccc tcagggaagg tgatcttgac cttgtccttg aaggcattgg caagacagat
     1681 catcccgtcg tgggaccatg ccggactgcc catccacttc cactcctcca caacgtcggg
     1741 atcagcttcg tggatgaccc tccgaatact ggcaagcgcc gcgccgcgcc agtcaccgag
     1801 actggcaatc aagccgtcga cgagttcggc tgcgttcatg gctgtaccac ctcccaaatt
     1861 gcgcgacccc atctgtggac caagccccgg cacaagtgcc agcgggcaga agacacactg
     1921 ccaggatgca cggaaggcga cctctccgca cgcacgctgt tcccctgcag cccggcgtcg
     1981 tgcacgtgac tactgacacc ctaggtccaa cggtatcgcg tgtcaaatcc aaaggcatct
     2041 ggcgaacgac cgcgagcaga gatggtcacg ccggcatgct gtggatttcc tttgctggta
     2101 tactgctgct gcaggatgaa gccagctttg atgaccgggg gagccgtgga acggaccaga
     2161 ctgatctccg aattgtggaa cgcgatggac aggcggcaat gggaggaact ggcagcgtac
     2221 ttctgcgacg acgctgtggt gttctggcct agcacgaacg agcgcttcac cgtccccgag
     2281 ttcattgagg tcaatcgcgc ctatcctggc acatggacga tccagatcga acgactccag
     2341 caggtcgacg accttgtcgt ttccgtcgtg aaggtcgcgt tggcagaggg ccgcacgtcg
     2401 tttcatgccg tgtcgttctt tgagttccga ggggacaaga tcggaacgct gtacgaatac
     2461 tggggcgaag atggcgatcc accccagtgg agaatcaatc gctcaatcgg aacacgcatc
     2521 tcctgaaacc ggggacctgt actttacgca gacagaaggg atacctgcta ttcagttctt
     2581 ctcccatccg gcgtgacatc tgtacccagt acgctgtctc agccgacctt tcgatggact
     2641 accaatggtt cctcgtgtgt ggcagagtct ccggcctcac ccgctgctat ccagaaaaca
     2701 ccagcgctgt ctcgtccgat ctccaatctt caccatccaa cttgaccaca atcccggttt
     2761 ggtgataatt agaccaaccg gtcggtttat gtgaccgaga ttgtttgacg acggtcgggc
     2821 actgggggcc acggtgaaac aggaagagcg ttcacaggaa acacggagcc gcatccttgc
     2881 ggtcgctgag cagagcttcg ctgatcacgg ctatgatgtg accagcgtcg acagtatctg
     2941 ccgggctgca ggcgtcagca agggggcatt ctatcatcat tttcccagca aggggtcggt
     3001 attcgtggca ctgctcaatg cctggctggg agacctcgac cggcagttcg cggcggcgca
     3061 ggctgggacc gaatctgtgc cacagcaggt agcgacgatg gctgcaggcg tcaacgagat
     3121 ctatcatatc gccggcacca aatggaacat cctgcttgag ttctgggcca agtccaaggc
     3181 ggatcctgaa gtggccgcca gcaccaccga catgctccac agataccagg ggttcttcca
     3241 ggagatgctg gaacggggca ggtcggaagg gagcctgaaa gtgaaggact ccagccttgc
     3301 cgcgaccgtc gcgctgagcg tcgtgctcgg gctgctgctc cagggcatcc ttgaccccga
     3361 gagtcaggat tggggggcgc tgatgcagcg cgccctggca atgctcatgg gatccatgag
     3421 tgggaggaac acatgagagt gctcgtaacg ggagcatttg gaaacgtcgg aacatatgcg
     3481 atcgatgagc tgctgagccg gggggcttct gtccggtgtc ttgacatccc gtcgcccatg
     3541 tcgaagcgga aagcccggcc ctatgccggc aagatcgaga tcctgtgggg cgacatccgc
     3601 aacacggatg tggtggcgcg agcggtgaag ggcgtcgacg ccatcgtcca cctggcattc
     3661 attatcccgc ccaggagcga gcagcgtccc gcgtgggccg agaccatcaa tgtcggcggc
     3721 acgtggaatc tccttcaggc cgcgcttgcc tcgaagagca gaccccgtat tgtcttcagc
     3781 tcctctattg ctctgtatgg ccgaacacag cacctcgcac caccccgcaa ggctgacgaa
     3841 ctgcttcacc cctatgagga ctatgcctat cacaagatga cctgcgagca catgctgcac
     3901 gcgtcaggcc tgccatgggt cattctccgg ttcggggccg tccctcccat cgagttcggc
     3961 gagctggacc cactgatgtt cgaggtgcgc atggacgacc gcatggagta cctggcgccc
     4021 atggacgccg gagcggcagt cgccgcagcg gcgctgctgc ccggattgga aggaaggacg
     4081 ctgatggtgg caggcggccc gtcctgtcag gtgaccggac gagagttcat gcagcgctca
     4141 ttcgatgcgc tggggatcgg gatgctgccc gaatcggcgt tctcacagat accgttccat
     4201 tgcgacttca tggacaccga ggagagcgag cggctgctgc actatcagca ccacagtttc
     4261 gacgaaacgc tggcgctgca gaggaagacg ttgggatgga agaagctgtt tgtgcccatt
     4321 gcacgaccgt tcatccgtcg gaagctgctg gccatgtccc cgtactatcg acgaaagatg
     4381 acagggagcg taccgctgcc gcagacagac cagacctgac acgctgcgtg gcatttcgta
     4441 ctatgaacga tatactaccc tcatagggta tggttcggcg tgtccatacg tgaaaacgag
     4501 ggggaaccgt ggggatgatt gcagcatgtg gactcgattg ctcgtcctgt cctgcctatc
     4561 tcaacaggaa tacgacggat caggctgtac tcgaagcggt tgcggcagag tggtcagccc
     4621 agctgcacca ggagatcaaa cccgagaatg ttccctgtga cggatgtcgg ctggccggag
     4681 aggcacgact gtccccgtat tgctacacct gtgccatccg tgcatgcgca cacggcaagg
     4741 gcttcgcgac gtgcgccgag tgtggaacat ctgaaacgtg cgcgacgctc cagaagttcc
     4801 ttgcagaggc tccatctgct gcactgacac tggcaagtct caggtcgagc cagaccagat
     4861 ccccggatga gtgacccagt ccgcgagtac tacttcatcg tcccggacgc gcaggacgct
     4921 catgttctcg tagtgcccga gggcggtgtc tggacgctgc cccacacgag catccccggt
     4981 gtccacaagt ggcagtcggt cgcacacgtc aacaaggcgg ccaacgaagc gctcggattt
     5041 cgtacgacca
//
LOCUS       DATMZD010000007         3975 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_110985,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000007 DATMZD010000000
VERSION     DATMZD010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110985"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            16..2514
                     /locus_tag="VN478_00125"
     CDS             16..2514
                     /locus_tag="VN478_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009226181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWQ21121.1"
                     /translation="MRVIVVGGNAGGASAAARLRRLDEKAEIVVYEKGEYISFANCGL
                     PYHIGGVITDRSQLLVQTATSMRERFNVEFLTRREVTSIDPQAHTVAVTDLATGTVTT
                     DHYDKLILSPGGSPLRPPIPGIESEGIYTLWTIPDMDSIVQAVHAGAKKAVVVGGGFI
                     GVEVAENLREQKVDVALVEMLPQVLAFLDPDIVAYAHQELQTHGVEMHLGDGVKSFAR
                     DAVGTLTVTLASGTQLPADMVVLAIGVRPNTQLAQSAGLTLGTSHGILVDEHLRTSDP
                     DIYAIGDAIEVEHFVTKNKTLIPLAGPANRQARIAADNIAGMDDTYTGTQGTSIVKVF
                     DLAAGATGANSALLTKLGIPFRSVTIHPGSHAGYYPGSTPVHTKLLYSPEGKLLGAQA
                     AGYDGVDKRIDVLATAIRLGATVDALTDLELAYAPPYGSAKDPVNMLGFVAQNDLQGL
                     TPAVTPEHLDEELAKGAVLLDVRQPEETDCGIIPGALTIPLPELRDRLAELPAGKKII
                     LTYCKVGLRGYVAQRILAQNGFQVANLTGGYTSWEASVNHGIEEQPTQPEREADDQQT
                     CTKEPCATKAALLTPPPAASIKTVEIDACGLQCPGPLLKMKEALAQTGPGDLIKVTAS
                     DPGFYPDVAAFAVAQGLELVDRQRGKLVTATLRRPAQAAIPTAGYTVVPGKSDDHATL
                     ILFSNDLDKVIAAFIIANGAQAMGKKVSIFFTFWGLNVLRKDENVRIKKTFIEKMFGM
                     MMPRGATHLILSKMHMMGMGTGMILGLMKKYNVTPVEAMMRSMLDGGGKFIACTMSMN
                     LMGIRREELIDGIEEGGVATMLGDADQGHINFFI"
     gene            complement(2585..3355)
                     /locus_tag="VN478_00130"
     CDS             complement(2585..3355)
                     /locus_tag="VN478_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HWQ21122.1"
                     /translation="MTYTIGKVTTMTHVSVRTLHYYDAIDLLNPSYETASGYRLYDDG
                     DLELLQQVLFFRALGFPLAQIKAIVQSPAFDRRKALEAHRTALLERRRTVDALLAAVD
                     HTIEAMGKGEAMDKKEMFDAFDERALEEHRTKYAEEAKQRWGMTDAYAESQKRTSKYT
                     KEDWARIQQEMAQIDADMAVLMDRKVTDPDVQDVVRRKHEYFNHNFYTCSAAMLAGLS
                     EMWVSDARFTATYDKIKPGLAQFWYDAVQICCTNSIDK"
     gene            complement(3476..3679)
                     /locus_tag="VN478_00135"
     CDS             complement(3476..3679)
                     /locus_tag="VN478_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013756123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HWQ21123.1"
                     /translation="MLTGKVKWFNAEKGYGFITRDDGDKDVFVHYSAILGSGFKSLKE
                     GDAVEFEVVQGDKGPQAKDVTIK"
BASE COUNT          827 a         1205 c         1159 g          784 t
ORIGIN      
        1 atcaaggagg atgaaatgag agtcattgtc gttggtggaa atgctggggg agcatcagcc
       61 gcagcgcgcc ttcgccgtct cgacgagaag gcggagattg tcgtctatga gaaaggcgaa
      121 tacatcagct tcgcgaattg cgggcttcca tatcacatag gcggcgtcat tacggatcgc
      181 agccagctgc ttgtccagac ggccacatcg atgagggaac ggttcaacgt cgaattcctg
      241 acgcgccgcg aggtgaccag catcgatccg caggcacaca cggtcgcagt aaccgatctc
      301 gctaccggca ccgtcacgac cgaccattat gacaagctga tcctgagtcc cggcggctcg
      361 cccctgcgcc cgcctatccc cggcatcgag agtgagggga tctatacgct ttggacgatc
      421 ccggacatgg acagcatcgt ccaggcagtc catgccggcg cgaagaaggc ggtggtcgtc
      481 ggaggcggtt tcatcggcgt cgaagtggcg gagaacctgc gggagcagaa ggtcgacgtt
      541 gcactcgtcg agatgctgcc acaggtgctg gcatttctcg acccggacat cgtcgcctat
      601 gcccatcagg aactgcagac gcatggggtg gagatgcatc tgggcgacgg cgtcaaatcg
      661 ttcgccaggg acgccgtcgg cacactgacc gttaccttgg ccagtggaac acagcttccg
      721 gcagacatgg tcgttctcgc catcggtgtg cgtccaaaca cgcagctagc ccagagtgcc
      781 gggctcacgc tcggtacctc tcacggcatc ctggtggacg agcatctgcg cacgagcgac
      841 cctgacatct atgccatcgg cgacgccatc gaggtcgagc acttcgtgac aaagaacaag
      901 acgctcatcc ctctggcggg cccggccaac cggcaggcac gcatcgccgc cgacaacatc
      961 gccggcatgg acgacaccta cactggcacg cagggcacgt cgatcgtcaa ggtcttcgac
     1021 ctagcggcgg gcgccaccgg cgccaactcg gcactcctga ccaagctggg cattcctttc
     1081 cggtcagtca ccatccatcc cggttcacac gccggatact accccggcag tacgcccgtc
     1141 cacacaaaac tgctctacag ccctgaaggc aagctgcttg gcgctcaggc agccggctac
     1201 gacggcgtcg acaagcgtat cgatgtcctc gcgaccgcga tccgcctcgg cgcgacggtc
     1261 gacgccctca ccgacctgga actcgcctac gccccgccct atggcagtgc caaggatccg
     1321 gtcaatatgc tcggcttcgt ggcacagaac gacctgcagg gactgacacc tgccgtgacg
     1381 cctgaacacc tggatgagga actcgcgaag ggcgccgtcc tcctggatgt ccggcagccc
     1441 gaggagactg actgcggcat cataccgggc gcgctcacca tcccgctccc cgaactacgt
     1501 gaccggctag ctgagttgcc cgcagggaag aagatcatcc tcacctattg caaggttgga
     1561 ctgcgcggct atgtggcaca acgcatcctg gcccagaacg ggtttcaggt cgccaacctg
     1621 accggcggct acacaagctg ggaggcttcc gtgaaccacg gcatcgaaga acagccgacg
     1681 cagcctgaac gcgaggcgga cgatcaacag acctgcacaa aggaaccgtg cgcgacgaag
     1741 gcggcgctgt tgacgcctcc tcctgcggct tccatcaaga cggttgagat cgatgcttgt
     1801 ggactgcagt gccctgggcc actcctcaaa atgaaagaag ctctggcaca gaccggtccc
     1861 ggcgatctca tcaaggtgac ggcatccgat cccggattct accctgacgt tgccgcgttc
     1921 gctgtggcac agggactgga actggtggac cgacagcgcg gcaagctggt cacggcgaca
     1981 ctgcgcagac ccgctcaggc ggcaatcccc acagcgggat acaccgtcgt ccctggcaag
     2041 agcgacgatc acgcgacgct gatcctgttc agcaatgatc tggacaaggt tattgcggca
     2101 ttcatcatcg ccaatggagc ccaggccatg ggcaagaagg tcagcatctt cttcaccttc
     2161 tggggcctga acgtgctccg caaggacgag aacgtgcgca tcaagaagac gttcatcgag
     2221 aagatgttcg gcatgatgat gccgcgtggt gccacgcatc tcatcctgag caagatgcac
     2281 atgatgggca tgggcacggg catgatcctg ggactcatga agaagtacaa cgtgacgccg
     2341 gtcgaggcca tgatgaggtc catgctggac ggtgggggca agttcatcgc ctgcaccatg
     2401 tccatgaacc tgatgggtat ccggcgcgaa gagctcatcg acggcatcga agagggcggc
     2461 gtggcgacca tgctgggtga cgctgaccag gggcacatca acttcttcat ctagagactg
     2521 cctgatgaat gcagaagatc cctccagcca cggctggagg gatcttctgt ttcagcagtg
     2581 tctgctactt gtcgatgctg ttggtgcagc agatctggac agcatcatac cagaactgcg
     2641 cgagaccagg cttgatcttg tcgtacgtgg cggtgaaccg ggcatccgac acccacatct
     2701 cgctcaggcc ggcgagcatt gccgcagaac acgtgtagaa gttgtgattg aagtactcgt
     2761 gcttgcgacg aacgacgtcc tggacgtccg ggtcggtcac cttgcggtcc atcaggaccg
     2821 ccatatcggc gtcgatctgt gccatctcct gctggatgcg cgcccagtct tccttcgtgt
     2881 acttcgacgt ccgcttctgg gactcggcat aggcatccgt cataccccat cgctgcttcg
     2941 cctcttccgc gtacttcgtc cgatgttcct ccagagcacg ctcatcgaac gcgtcaaaca
     3001 tctccttctt gtccattgct tctcctttgc ccatggcttc tatggtgtga tcgactgctg
     3061 caagaagcgc gtcgactgtc ctgcgccgct caagcaatgc ggtgcggtgt gcttccagcg
     3121 ctttgcgccg atcgaatgcc gggctttgca cgatcgcctt gatctgggcg agcggaaacc
     3181 cgagcgcgcg gaagaagagg acctgctgca gcaactccag atcgccgtcg tcatacaacc
     3241 ggtacccgga agctgtctcg tacgaagggt tgagcaggtc gatggcgtcg tagtaatgaa
     3301 gcgtgcgcac gctcacatgg gtcatcgttg tcaccttccc gatggtatag gtcatcgctg
     3361 ctcctttcca tgccatggta gcatagagta tgacgtaacg tcatagtcaa gaggccgggg
     3421 tatgcgaaag ccccggcttt cgccggggct gattgggttc agtattggga aaagactact
     3481 tgatcgtaac gtccttggcc tgcggaccct tgtcgccttg cacgacttcg aattcgaccg
     3541 cgtcgccttc cttcagcgac ttgaaacctg aaccgaggat ggcgctgtag tggacgaaaa
     3601 cgtccttgtc gccgtcgtca cgagtaatga aaccataacc cttctctgcg ttgaaccact
     3661 tgactttgcc tgtaagcact gaaacctcca taaaacttgg catgtactat cttgccatag
     3721 ggcagtatat agggtaagtt ccccgatgca agtgctgcgt cgcaaatcag tcattcatta
     3781 tatgttcgca ttgtatcctg gtgtgtcatt tcggtgaatg gccggacaga agctgatgtg
     3841 ccggcgggcg ctgccctcaa acgaaagccc cggcgaaagc cggggctgat tgggttcagt
     3901 attgggaaaa gactacttga tcgtaacgtc cttggcctgc ggacccttgt cgccttgcac
     3961 gacttcgaat tcgac
//
LOCUS       DATMZD010000008         2780 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_101056,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000008 DATMZD010000000
VERSION     DATMZD010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101056"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VN478_00140"
     CDS             <1..412
                     /locus_tag="VN478_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548170.1"
                     /note="protein from Staphylococcus aureus has
                     phosphodiesterase activity against 2'-3'-cAMP and
                     2'-3'-cGMP; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HWQ21124.1"
                     /translation="IGADILNKSGESPDIVHAVAAHHTDVPMETVLDVIVHVADALSA
                     SRPGARRESVESYIKRLEKLESIANSIPGIKKSYAIQAGRELRILVEPDEVDESLAAK
                     IAMDVARKVELEVSYPGQIKVTVVRETRYSDYAK"
     gene            381..2075
                     /gene="uvrC"
                     /locus_tag="VN478_00145"
     CDS             381..2075
                     /gene="uvrC"
                     /locus_tag="VN478_00145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HWQ21125.1"
                     /translation="MRPDTLITPSSLLEGLDTAYKLPFEKDSLGKLPVMPGVYVIRDS
                     SDRIIYVGKAKNLRKRVESYFRSDLTPKTRSMVGNARFFSFITAGHEDQALVLESNFI
                     KCYKPHYNIQLIDGKSYPLIELTGEAFPVLSKTRRVTTARSHYFGPYPKSGLLNLGLD
                     SLRHAFPVRTCHRRIDAARPTKPCLDFDLGLCLAPCGNRCSHDEYARAVQNLSEFLNG
                     KMRTLLRQLENDMDQASRSQQYERAVRYRDSYLALKGLFERYAVFAPRARDMDVFAFH
                     QQGNLVAVVRQALREGRLIASTEFIYDLKGKDALDSTARADLIIDFYQRFRVAAPNRI
                     AVEVVGSEAGIVRERLRAALGTSISIASPRSTDTSRLLRFAAENAEQKLIASTRAADV
                     PAQLSVLKAILDLEHLPVRIEGYDVSNISGKDATVSMVVFVAGRPAKDQYRLFNIRSL
                     DTPNDPAMLAEALARRFARWDDVAFGDQASLLLIDGGTSQLGAVCTVRDKAGVHVPVV
                     GIAKKEELLYREGTSDPVRLPHDSGALLLLMAIRDEAHRFGKREFHKRHERPLKHR"
     gene            2092..2448
                     /locus_tag="VN478_00150"
     CDS             2092..2448
                     /locus_tag="VN478_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HWQ21126.1"
                     /translation="MNLIIRLLLNASVFLLVSRVVPGFQVASLWTALWAALIFGFVNA
                     LIRPILLAVSLPLSILTLGLFTLVVDAGVMALTAWLVPGFDVHGFLAALLGWVLVSLG
                     SMVVSSLLGDNGAKRA"
     gene            2445..>2780
                     /locus_tag="VN478_00155"
     CDS             2445..>2780
                     /locus_tag="VN478_00155"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HWQ21127.1"
                     /translation="MSPKMLGDFHVHTVFSGHAFSTLQEVVVAAQQRGLKYLAITDHG
                     PARPDGPKLDYFKNLNRQDTLRQLSGIRVLIGVEANIINPWGDLDMPDKLLHELDVVI
                     AGFHNSTGYG"
BASE COUNT          550 a          789 c          829 g          612 t
ORIGIN      
        1 gattggggca gacatcttga acaagtcggg tgaatcaccg gacattgtcc acgctgtcgc
       61 ggcacatcac accgacgttc cgatggagac cgttcttgac gtcatcgttc acgtcgcaga
      121 cgctctctcg gcgtcgcgac cgggcgctcg ccgtgagagc gtcgagagct acatcaagcg
      181 tcttgagaaa ctggagtcta tcgccaactc gatacccggg atcaagaagt cgtatgccat
      241 acaggcgggg cgtgagctcc gaatcctcgt ggagcccgat gaggttgacg agtcccttgc
      301 agccaagatc gccatggacg tggctcgcaa ggtggaactg gaagtctcgt accccgggca
      361 gatcaaggtg accgtcgttc gtgagaccag atactctgat tacgccaagt agtctccttg
      421 agggcctgga tacagcatac aaactgcctt ttgagaagga ctctctcggt aaactcccgg
      481 tcatgccggg ggtttacgtc atacgggatt cttctgatcg gatcatctat gtaggcaaag
      541 ccaagaatct ccgcaagagg gtggagtcgt acttcaggag cgacctgacg ccgaagacca
      601 ggtcgatggt gggcaatgcc cgcttcttca gtttcatcac ggcagggcac gaggatcagg
      661 ctctcgtgct cgagtccaac ttcatcaagt gctacaagcc tcactacaat atccagctca
      721 tcgacgggaa gtcgtaccct ctgatcgaat tgactggtga ggcgtttcca gtactcagca
      781 agacccgccg cgtgacgact gccaggagcc actatttcgg tccctacccg aagtcagggc
      841 tgctgaacct gggcctcgac agcctgcggc atgcgtttcc cgtcaggaca tgtcatcgaa
      901 ggatcgatgc ggcgaggccc accaagccat gtctcgactt cgatctgggt ctgtgcctgg
      961 ctccgtgtgg caacagatgt tctcacgatg aatatgcccg ggcagtccag aatctgtcgg
     1021 agttcctgaa cggcaagatg cgtacgctgc tcaggcaact cgagaacgac atggatcagg
     1081 cgtcccggtc gcagcagtac gagagagccg tacgttatcg cgactcgtac ctggcgctga
     1141 aggggctatt tgagcggtac gccgtgtttg ccccgcgagc gcgagacatg gacgtcttcg
     1201 catttcatca acaggggaat cttgtggctg tggtacgtca ggcacttcgt gaggggaggc
     1261 tgatcgcctc gaccgagttc atctacgatc tcaagggcaa agacgctctg gattcgaccg
     1321 cgcgggcgga tctcattatc gatttctacc agcggtttcg tgtggctgct ccgaaccgga
     1381 tagccgttga ggttgtgggg tctgaagctg gtattgtccg cgagcgactg cgggcggctc
     1441 tcggaaccag tatctccatc gcatcgccgc gttcgacaga tacctcgcga ctactgcgct
     1501 ttgcggcgga gaatgcggaa cagaagctga tcgcctcgac gcgggcggcc gacgttccgg
     1561 cccagttgtc agttctgaag gcaatactgg acctggaaca ccttcctgta cgcatcgagg
     1621 ggtacgacgt ttcaaacatc tcaggcaagg atgctactgt atcgatggtc gtattcgtcg
     1681 ccggcaggcc tgcaaaggat cagtacaggc tgttcaacat tcgctcactg gacacgccga
     1741 atgacccggc tatgctggcg gaggctctcg cccggcgctt cgcaaggtgg gatgacgtgg
     1801 cgtttggcga tcaggcgagc ctcctgctga tcgacggtgg gacctcgcaa ctcggggctg
     1861 tctgcactgt gcgcgacaag gccggcgtgc acgtgccggt tgtgggtatc gcgaagaagg
     1921 aagaactgct gtatcgtgaa ggaacgtcgg atccggtacg actcccgcac gattccggcg
     1981 ctctattgct actgatggcg attcgcgacg aagctcaccg ctttggcaag cgcgagttcc
     2041 acaaacgtca cgagcgtcct ctgaagcacc gctgatcctg aaaggagaga catgaacctc
     2101 attattcgac tgctgctcaa tgcctcagtc ttcttgctcg tcagccgggt cgtccctggt
     2161 tttcaggttg cctcgctgtg gacggcactt tgggccgcgc tcatctttgg gttcgtcaac
     2221 gcactgattc gtccgatcct gctcgctgtc agccttcccc tgagcatact gacccttggc
     2281 ctgttcacgc tggtggtcga cgcaggcgtg atggctctga cggcgtggct ggtccctggc
     2341 tttgatgtgc acggtttcct tgcggcgctt cttggctggg tactcgtttc gctggggagc
     2401 atggtagtct catccctcct cggagacaac ggagcgaagc gggcatgagt ccaaagatgc
     2461 ttggagactt ccatgtccac actgtctttt cggggcatgc gttctcgaca ctgcaggaag
     2521 tggtcgttgc ggctcagcag cgtggcctga agtatctcgc catcactgat cacgggcccg
     2581 cacgccccga tgggcccaaa ctggactact tcaagaacct gaacaggcag gacacactgc
     2641 gtcagttgtc tggcattcgg gtgctcatcg gggtcgaggc caacatcatc aacccctggg
     2701 gagacctgga catgcccgac aagttgttgc atgaacttga tgtcgtcatt gctggattcc
     2761 acaactccac aggttatggg
//
LOCUS       DATMZD010000009         2208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_120983,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000009 DATMZD010000000
VERSION     DATMZD010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120983"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1260
                     /locus_tag="VN478_00160"
     CDS             <1..1260
                     /locus_tag="VN478_00160"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HWQ21128.1"
                     /translation="SDYDLIVIGGGPAGLAAATAAYDQGLHDILIIERDRFLGGILNQ
                     CIHTGFGLQEFREELTGPEYAQRFVDRTLQDGIPFLLDTMVLALRSDRTVVAISPELG
                     RLELHARAVVLSMGCRERTRAAIMVPGTRPAGVMTAGTAQRYMNVEGYLPGRNIVILG
                     SGDIGLIMARRFTLEGARVNAVLEIMPYSTGLTRNVVQCLDDYNIPLLLSHTVTSVHG
                     GKRVTGVSIAQVDEHRCPVPGTEREIPCDTLITSVGLVPENELSKTAGVQLDAITGGA
                     LVNQRLQTSVEGVFACGNVLQVHDLVDNVSEESQRAGRSAALYVQGKLPRTVRRMRLV
                     PAPGIRYIVPQYIDVTEQPESVKLFMRPTDVFKGASIELWTDKQKAPLARFHRTRLTP
                     GEMVVLTVQGSVFAAAETDVMVEVEVL"
     gene            1257..1634
                     /locus_tag="VN478_00165"
     CDS             1257..1634
                     /locus_tag="VN478_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017751903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HWQ21129.1"
                     /translation="MNTEVCEVTCIVCPTGCKVRVVKEGTRVIEVSGNTCKRGEVYAA
                     QEAVAPRRTLTTSIKVTGGDFALVSVKSAAPVPKGRLFDVMKAVQKLQMTAPIQVGDV
                     VVHDVLGLGVDLVATRAVHRTAQ"
     gene            complement(1711..>2208)
                     /locus_tag="VN478_00170"
     CDS             complement(1711..>2208)
                     /locus_tag="VN478_00170"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HWQ21130.1"
                     /translation="GGPAIVPNLQRALKAARSANESIVHVTRRHRTSGIDVDQGRRAL
                     FAKTGGFLVAGTPGAEELPEFAPVPGDFAVVKTRWSGFFATDLDALLRRLGIHKLVLT
                     GVQTPNCIRATANDALSLDYATIVLSDATASQTDEIQQANLRDMAVMGVDIMTVSQYE
                     QSPGL"
BASE COUNT          433 a          629 c          715 g          431 t
ORIGIN      
        1 agtgactacg atctgatcgt catcggtggc ggcccggccg gactggcggc agcgaccgca
       61 gcgtatgacc agggactgca cgacattctg atcatcgaac gggatcggtt tctgggtggc
      121 attctgaacc agtgcattca cacggggttt ggactgcagg agttcagaga agagctgacc
      181 gggccggaat acgcccagcg attcgtcgac aggacccttc aagacggtat cccgtttctc
      241 ctcgatacga tggtgcttgc tctacggtcg gaccgcacgg tcgtggcgat ctcgccggaa
      301 ctgggcaggc tcgagctgca cgccagggcg gttgttctct cgatgggctg ccgcgagcgt
      361 acacgagcag ccatcatggt tcctggcacc aggcccgcgg gcgtcatgac ggccggcacg
      421 gcccaacgct acatgaatgt ggaaggatac cttcccggcc gcaacatcgt catcctcggt
      481 tccggtgaca tcggtctcat catggcgcga cggtttacgc tggaaggggc acgtgtcaat
      541 gccgttcttg agatcatgcc ctactcgacg ggtcttaccc gcaatgtggt acagtgcctc
      601 gacgactaca acattcctct gctgttgagc cacaccgtga cgagcgtgca cggcggaaag
      661 cgcgtgacag gggtgtccat cgctcaggtg gacgagcaca ggtgcccggt cccgggaacc
      721 gaacgcgaga taccatgtga tacactgatc acctccgtcg gacttgtccc cgagaatgag
      781 ctcagcaaga cggcgggcgt ccagctggac gccatcaccg gtggcgccct cgtcaaccag
      841 aggcttcaga cgtcggtcga aggggtcttc gcctgtggga atgtcctgca ggtgcatgat
      901 ctggtcgaca atgtatccga ggagtcgcag cgcgctggca ggagtgccgc gctatatgtt
      961 cagggcaaac tcccaaggac ggtgcgcaga atgcggttgg ttccggcgcc cgggatccgc
     1021 tatatcgtgc ctcagtatat cgatgtgacg gagcagccgg agtccgtcaa gctctttatg
     1081 cgtccaactg acgtgttcaa gggtgcctcc atcgaactct ggaccgacaa gcagaaagct
     1141 ccactggcca ggttccatcg cactcggctg acacccggtg agatggttgt gctgacggtc
     1201 cagggaagcg tgttcgcggc cgccgagact gacgtcatgg tggaggtaga ggtcttatga
     1261 acactgaggt gtgcgaagtc acatgtatcg tttgcccgac ggggtgcaag gtacgtgtcg
     1321 tcaaagaagg gaccagggtc atcgaagtgt caggcaatac gtgcaagcgc ggcgaagtgt
     1381 atgccgctca ggaggcggtc gcaccccgac gcacgctgac gaccagcatc aaggtgacag
     1441 gcggagactt tgcactcgtg tcggtcaaga gtgcggcgcc ggtccccaag ggtcgactct
     1501 tcgacgtcat gaaggcggtc cagaagcttc agatgacggc accgatccag gtgggggatg
     1561 ttgtcgtcca cgacgtgctc ggattgggag tggacctggt ggctacgcgg gccgtgcaca
     1621 ggaccgccca ataggcgggg cctcaggctg caggaacttg cagccggtcc atagagggcc
     1681 cgctgtcatc ttccgaggca gtagggggga ctagaggcct ggcgattgct cgtattgtga
     1741 gacggtcata atgtctactc ccatgacagc catgtctctg aggttggcct gctggatctc
     1801 atcggtctgg gatgctgtgg catctgagag cacgatcgtc gcgtagtcca gcgagagagc
     1861 atcgttcgct gtcgcgcgta tgcagttggg tgtctgcact ccggtcagga cgagtttgtg
     1921 gataccaagc cggcgcaaca gagcatccag atcagtcgca aagaatcccg accagcgcgt
     1981 cttgacgacg gcgaaatccc cagggacagg cgcaaactca ggtagttctt cggctccagg
     2041 agtccctgcc accagaaacc ctccggtctt cgcgaagagt gctcgccgac cctggtcgac
     2101 atcgatcccc gaggttcgat ggcggcgagt cacatggaca atcgactcgt tggcggatcg
     2161 ggcggctttc agtgctcttt gcaggttcgg aacgatggcc ggtccccc
//
LOCUS       DATMZD010000010         3159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_396,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000010 DATMZD010000000
VERSION     DATMZD010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..347)
                     /locus_tag="VN478_00175"
     CDS             complement(30..347)
                     /locus_tag="VN478_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21131.1"
                     /translation="MLELPQNIMAKVEENVRRYGERSQWLLQWYDPGHIGKPMSASQV
                     MHTITEEARLQGISMTCRSIRVAGGVDALRSRHEPVEDVLRRLGGDRWRGELTKALGE
                     KRS"
     gene            complement(401..847)
                     /locus_tag="VN478_00180"
     CDS             complement(401..847)
                     /locus_tag="VN478_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HWQ21132.1"
                     /translation="MEERLIAPCGMNCGVCVSYLAMKCNLNKQGFQKSYCPGCLPRGK
                     NCAFLKKRCSLLGKGLIRFCFECGAFPCSRLNALDKRYRTRYHMSMVENLVFIKEHGI
                     AEFLGQEEAKWRCPDCGGAICCHNGLCLNCHLDKLRQNKKYCWDEE"
     gene            1094..2089
                     /locus_tag="VN478_00185"
     CDS             1094..2089
                     /locus_tag="VN478_00185"
                     /inference="COORDINATES: protein motif:HMM:NF024640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HWQ21133.1"
                     /translation="MDDWRRQLRFDPLLPLLASDSEALQYMVRRDLLGERVGSTGCLS
                     ELPAVQRILRHQLEDGSWAGSGEHKHPAINVCLIETWRQFRCLVEQYGMTREHPRVQK
                     AAEYLFSCQTLVGDFRGMLANQYATYYTGAIMAVLIEAGYADDPRIEKGFQWLLSMRQ
                     DDGGWSIPLITHKFDRATQYRLTSEYADPVEPDRSRPFSHNWTGMVLRAFAVHPQERR
                     SSAAMVAAALLKSRFFQPDPYTSYHAATYWVRFQYPFWWNSLVSALDSLSLMGQPKED
                     LQIQKALAWLAEHQQQDGLWRVSYVKTQEQETAKTLEMRLWVTLAICRTLKRLYG"
     gene            complement(2117..3064)
                     /locus_tag="VN478_00190"
     CDS             complement(2117..3064)
                     /locus_tag="VN478_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020768521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HWQ21134.1"
                     /translation="MNGVVYDKTSPDHLSYCDVEKPVPGDGEVLIRVHTTSLNAADYR
                     LFKMGLGIPKTGIFGADVAGTVESVGKNVLTFKPGDDVVADTSGCGFGGMAEYARASQ
                     DTAVRKPTGVSFEDAAASPMASVTALQALRDIGRVREGQKVLIHGASGGVGTFAVQLA
                     KCYGADVTAVCSTGNVQRIRDLGADHVIDYKIEDFTRNGIRYDLIVAINGYHPLSAYK
                     RSLTSAGTYVMIGGTFPQIAQSLLFGPMMSLGTRKMRSLAAKANSKDLAFIMELLQER
                     KLHPVIDRTFPLAEGAAAFLYIASGHAFGKVLIRVAGET"
BASE COUNT          631 a          973 c          855 g          700 t
ORIGIN      
        1 gaccccctcg agggcggttt cccgtccggc tatgaacgtt tttcgccgag agctttcgtc
       61 agctctcctc tccatctatc cccgccaagt ctccgcagga catcctccac gggctcatgg
      121 cgggatctca gtgcatcaac gcctcctgcc acacggatgc tgcggcacgt catcgagatc
      181 ccctggagcc gcgcctcttc cgtaattgtg tgcatgactt gactcgcgct catcggcttg
      241 ccgatgtgac cggggtcata ccactggagc agccattgac ttctctcgcc ataccttcgg
      301 acattctctt cgacttttgc catgatgttc tgcggcagtt ccagcacagt gtccctccgg
      361 ttggcaacga gctaaaccgc tcggagtcgt tctgtcctgt ctactcctca tcccagcagt
      421 acttcttgtt ctgacgcagc ttgtcgaggt ggcagttcaa gcacagcccg ttgtgacagc
      481 atatcgcccc gccacagtca gggcagcgcc actttgcctc ttcctgcccc aggaactctg
      541 cgatgccatg ctctttgatg aatacaaggt tctccaccat actcatatgg tatctggtgc
      601 ggtatcgctt gtcgagggcg ttcagccgac tgcaggggaa tgctccgcac tcaaagcaga
      661 accggatgag tcctttcccg agcagactac accgcttctt caggaaggcg cagttcttgc
      721 cgcgtggcag gcagccaggg cagtacgact tttggaatcc ctgcttgttc agattgcact
      781 tcattgccag gtagctgaca cagaccccac agttcatccc gcaaggagca atgagtctct
      841 cttccatctc ccgtcaccgc ctcctgtcca gaagcatcat ctgatgaata acgcatatat
      901 cttcggcaat cgcgcccttc gtacaagaga agagagcctc agacatgatt ctgaggacaa
      961 cggtgaagca cggaaccggc ccatagccgt ctttcgggca gcgcaattca gaacatagta
     1021 gtcgcatcac gggaagagat ggccgatgtg cgggcagtga tcgtgcgaac agcgaccgat
     1081 gaggaggtcg gtggtggatg actggcggag acaactgagg ttcgaccctc tcctgccgct
     1141 cctggcctct gacagcgagg cgctgcagta catggtacgc cgtgatctgc tgggtgagcg
     1201 ggtcggctcg actggctgcc tgtcagagct gcctgcagtt caacgaatcc tcaggcacca
     1261 acttgaggac gggtcctggg ccgggtccgg cgagcacaag catccagcca tcaatgtctg
     1321 tctcatcgag acgtggcggc agttcagatg cctggtggag caatacggca tgacccggga
     1381 gcatccccgg gttcagaaag ccgccgagta tctgttctcc tgccagacac tcgtcggcga
     1441 cttccggggc atgctggcca atcaatacgc gacctactac actggtgcga tcatggctgt
     1501 cctcatcgag gcggggtatg ccgatgatcc ccgtattgag aagggcttcc agtggctttt
     1561 gtccatgcgt caggacgatg gcggctggtc aattcctctc atcactcaca agttcgaccg
     1621 agccacgcag taccgactga cctcggagta tgcggaccct gtcgagcccg atcgctccag
     1681 acccttctcc cacaactgga cgggcatggt actgcgagcc tttgccgtcc atccgcagga
     1741 gcgtcgctcc agcgctgcga tggtcgccgc agcgctactg aagtcccgat tcttccagcc
     1801 ggacccctac acttcctatc acgccgccac ctactgggta cggtttcagt accccttctg
     1861 gtggaacagc ctggtctctg cgctcgattc cctctctctg atgggccagc ccaaggagga
     1921 cctgcagatc cagaaggcgc tggcctggtt ggcagaacac cagcagcagg atgggttgtg
     1981 gagagtgtcg tatgtcaaga cccaggagca ggagaccgca aaaacgctgg agatgaggct
     2041 gtgggttacg ctggcgatct gtcgcacatt gaagaggctg tacggatagg ttcccagggt
     2101 cttgcacgaa ttccggtcag gtttcccctg ctacccgaat cagcaccttg ccgaaggcat
     2161 gaccggatgc aatgtaaagg aaagcggcag caccctcggc gagtggaaat gtcctgtcga
     2221 tgacgggatg cagctttctc tcctggagaa gctccatgat gaacgccaga tccttgctgt
     2281 tggcctttgc cgccagagaa cgcatcttcc ttgtgcccag tgacatcatc ggtccaaaga
     2341 gcagggattg ggcgatctgg gggaacgtgc cgccgatcat gacgtaggtg cctgcagacg
     2401 tcaaagaccg tttgtacgcc gacaacggat gatagccgtt gatggcaacg atgagatcgt
     2461 atcggattcc gttcctggtg aagtcctcga tcttgtagtc gatcacgtgg tccgccccaa
     2521 gatcccgtat cctctgtaca tttcctgtgc tgcatacagc tgtaacgtcg gcaccgtagc
     2581 acttggcaag ctggacagca aaggtcccca caccaccgct tgccccgtgg atcaggactt
     2641 tctgcccctc cctgacacgt ccgatatcgc gcagcgcctg gagagccgta acagacgcca
     2701 tcggcgaagc cgctgcatct tcaaaagaca ctcccgtcgg cttgcggacc gccgtatcct
     2761 gcgaagctcg agcgtattct gccatgccgc caaatccgca tccggaagta tccgccacga
     2821 cgtcgtctcc tggcttgaat gtcagaacgt tcttaccgac tgactctacc gttccggcaa
     2881 catcagcccc aaatatcccg gtcttcggta tgccaagtcc cattttgaag agacgatagt
     2941 ccgccgcatt cagtgaggta gtatggaccc tgatcaggac ttctccgtcg ccgggcacag
     3001 gcttttcaac gtcacagtag gaaaggtgat cgggggatgt cttgtcgtag accactccgt
     3061 tcatggcaca ttcctcatgt tctgattcct tcctctatcg ctgccggtcc tcatcacgta
     3121 ctccccttgg tcttgtaccc gaacacctca gtgatcggc
//
LOCUS       DATMZD010000011         5254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_70836,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000011 DATMZD010000000
VERSION     DATMZD010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5254
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70836"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..1904)
                     /gene="cas6"
                     /locus_tag="VN478_00195"
     CDS             complement(99..1904)
                     /gene="cas6"
                     /locus_tag="VN478_00195"
                     /inference="COORDINATES: protein motif:HMM:TIGR01877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HWQ21135.1"
                     /translation="MRIRIEVQSALDKFVLPQGYNPYVQGLVNACIPEDIKWSGIATG
                     TEEAHFKLFTFSQLYAKSVERIDIAPRDGVESDGHVHYQLVMSSPVWFYLSSPSPEFI
                     EALVPRLEQAHDLHLERNLVQSVRVVPAYPASIPTHGLVTWRIRTISPITVYQSESKD
                     SNSAHYFAPDDPLFAKLVLDNAVRKYYAWTQEKAPSDGFSITCLDHTPHLAVISFKDT
                     PVHGYTGRFQLTGDAKLLSFLYESGLGGKNSHGFGMFDIAPEGEDDAHWRRRTEEETR
                     VVRRAPSAPMAAADQDRPDRHERSDNRGQEGHVWHGEHHGATGQTGSSKSAGSRDDRT
                     HTSRPHTDRPASDHPRTDRPYSDRPHTEHSVSDRPHTDRPTSDRPHTDRPYSDRPRTD
                     RPHTERSASDRPRTDRPASDRPHTDRPYSDRPRTDRPYSDRPRTEEPRLDGTPGTSRG
                     TGRPGNDNRRFQSSPRSGTSDRRGPRGTEEPRRKFVSRYAPDIKPVSHEKAATGSYSI
                     WDGKAKTELPRFVRPDGTESTGGPRPNEHRGGPRPGASGGRPSSGAGRPQGDRRGGSF
                     GAARPDGSSGSVHRPSGGHGDPRDHRGSKGGPHSR"
     gene            2126..3397
                     /locus_tag="VN478_00200"
     CDS             2126..3397
                     /locus_tag="VN478_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020879130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HWQ21136.1"
                     /translation="MRTRIRTLFDGHTPHLQTLELLKYIGPGFLVTVGFIDPGNWASD
                     VAAGSLYGYRLLWVVTLSTLMLIVLQHNAAHLGIVTGLCISEAATKFTHRRFSRLVLG
                     SAVVACVSTALAEILGAAIGLDLLFGLPLRVGAVMVTAAVTWLLLSNGYRRIEKVIIA
                     FVSLIGLSFLYELSLVHIPWAEVSAGWVTPAIPLGSIPVVMSVLGAVVMPHNLFLHSE
                     IIQSRQLNRDGTDVIEKQLRFEFVDTLNSMVIGWAINSAMVILAAATFFAARTPVTEL
                     GQAEAMLRPLLGKAAAVVFGGALLLAGLSSSVTAGMAGGSIVAGMSGEPYDPSDNHTR
                     LGVLMTLLGGLLIAIVITNPFRGLIISQMALSVQLPWTIVLQIMLTSNPRVMGRYVNT
                     SLDRALLWTTAIVVTGLNVLLLVDTLRNLHH"
     gene            3535..4542
                     /locus_tag="VN478_00205"
     CDS             3535..4542
                     /locus_tag="VN478_00205"
                     /inference="COORDINATES: protein motif:HMM:NF024083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HWQ21137.1"
                     /translation="MTNSTPDARWRRQIIVVLAIMLATASSIVLCPAPDVSAGTYNPL
                     KAVQYANQWWNKRNPKFQSYGFNDCANYVSQCLIAGGLSLRASPSADKSGSLAQCTSL
                     DAYLTNTLKATVWKTSSSARPPTALQPGDVAIFGNWLGLRHAVFVSAKQTNGQPLFNA
                     HTYDRLRTTIDWLFSAWTYVKYYHIVKSPITVAVAAKPAAPAKTAVVAQPAVTTRSSI
                     AEPKPEPVETAALVKTVPASSVTSTSALLHGIVRPDGQTTLWFFEYGVSEDYDEATDK
                     QGPLLGNMTYDVDLEVAGLKESTLYHARLVRVAPDGSLTTGSDITFKTTEAPHSVTRQ
                     D"
     gene            complement(4603..>5254)
                     /locus_tag="VN478_00210"
     CDS             complement(4603..>5254)
                     /locus_tag="VN478_00210"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWQ21138.1"
                     /translation="PQGERSLLDVCCGTGQLALQFLQAGYVVTGLDNSEPMLAHARMN
                     CASFVSSGEVSFVQKDAADFSLSSRFGFAVSTFDALNHLSGIDALLSCFACVHQCLLP
                     GGLFVFDLNTVMGLRRWNNVTVEDALDAMVINRGMYDEDMRQGRIKISGFVRQESGSW
                     LRFDETFVENAWSVREVTTALLEVGFSAVYQARAAELTAPLADPESEPRVFFVARG"
BASE COUNT         1022 a         1639 c         1531 g         1062 t
ORIGIN      
        1 tccttattcg tactacgaac aagaaatgct cgggacctcc catgcgaaag ggaggcgcct
       61 tccggcgcct ccctgtatct ggtgatactc aatcgaagtt agcggctgtg gggaccaccc
      121 ttgctgcccc tgtggtcacg cggatcgccg tgtcctccac tgggacggtg tacggaacca
      181 gagcttccgt ctggcctcgc agcgccaaag cttccgccac gtctgtcgcc ctgaggacgt
      241 ccagctccgc tggaaggacg gccgccggat gcgccagggc gaggccctcc gcgatgctcg
      301 ttcggacgag gtccaccagt actctcggtg ccgtcgggac ggacgaaacg aggcagttcg
      361 gtctttgcct tcccatccca gatgctgtag gaaccagtag ccgccttctc gtggctcacc
      421 ggcttgatgt ccggggcata ccggcttaca aacttccgac ggggttcctc tgtgccacga
      481 ggacccctgc gatccgacgt gccgctccgc ggagaggact ggaaccttcg attgtcattg
      541 cctggacgcc cggttccacg actggttccc ggggtaccgt cgagtcgagg ctcttccgtc
      601 ctgggacgat cagagtatgg acggtcggta cggggacgat cagagtatgg acggtctgtg
      661 tgtggacgat ctgaagccgg tctgtcggtg cgcggacgat cagaggcaga gcgttctgtg
      721 tgcggcctgt ccgtccgggg acgatcagag tatggacggt ctgtatgcgg gcgatccgaa
      781 gttggtctgt ctgtgtgcgg acgatccgag acagaatgct ctgtatgcgg acgatcagag
      841 tatggacggt ctgtgcgcgg atggtctgaa gccggtctgt cggtgtgcgg acggctggta
      901 tgcgtgcgat cgtcccggga tccggcactc ttcgatgagc cggtttgtcc tgtggcgccg
      961 tgatgctcac catgccacac atgtccttcc tgaccacgat tgtccgaccg ctcgtgacgg
     1021 tccggcctgt cctgatcggc tgctgccatg ggcgccgacg gagctctgcg cacgacgcga
     1081 gtttcctctt ccgtcctgcg acgccagtga gcatcgtcct ctccctccgg agctatgtcg
     1141 aacatcccga aaccgtggga attcttgccc ccaaggccag actcgtacag gaacgacaac
     1201 agcttggcat ctcctgtcaa ctggaagcgc ccagtatagc cgtgcaccgg cgtgtccttg
     1261 aacgaaatga cggcgagatg cggagtgtga tcgagacatg tgatgctgaa gccgtcgctg
     1321 ggggctttct cctgtgtcca cgcgtagtac ttgcggaccg cgttgtccag gacgagcttg
     1381 gcgaacagcg gatcatcggg cgcgaagtag tgcgccgagt tgctgtcctt gctctcgctc
     1441 tggtacaccg taataggact gatcgtgcgg atcctccatg tgaccagccc atgagtgggg
     1501 atgctggctg gatacgcagg caccacacgc accgattgca cgaggttccg ttcaagatgg
     1561 aggtcatgcg cctgttccag gcggggcacc agcgcctcga tgaactcggg actgggagaa
     1621 gacaggtaga accagacagg cgacgacatc accagctgat agtgaacgtg tccgtcggat
     1681 tcgactccgt cacgaggagc aatgtcaatg cgctccacag acttcgcata cagctgcgag
     1741 aaggtgaaga gcttgaaatg ggcctcttcc gtgcctgttg cgatgccaga ccacttgatg
     1801 tcctcgggaa tgcacgcatt cacaagtccc tgtacgtagg ggttgtaccc ctgaggcagg
     1861 acgaacttgt cgagagcgct ctgtacttcg atcctgatac gcattgaaac ctccactcaa
     1921 ttcagagaac agacaaccgg aaaggtgagc cattacggcc gtgacgcgcc ccggcgaccg
     1981 gggtgctcat tcgaatgtcc tgctgtagtc atagtattat aggagttgtc aagcgccgtg
     2041 caactacaac ttcaagagga acagtcaaga cctgcgtgca gacgcttgat gacgcgtctg
     2101 gaccaactat actcaggtca tgagtctgcg cacacggatc cggacgctgt tcgacggcca
     2161 cactccacat ctccagacgt tggagctcct gaagtacatt ggccccggat tcctcgtcac
     2221 cgtcggcttc atcgatccag gcaactgggc atccgacgtt gctgcaggtt cgctctacgg
     2281 ctatcgtctt ctgtgggtgg tgacactctc gaccctgatg ctgatcgtcc tgcagcacaa
     2341 tgctgctcac ctcggcatag tcaccggtct gtgcatctcc gaagctgcca cgaagttcac
     2401 tcaccgccgg ttctcccgac tcgtcctggg ctctgctgtc gtcgcctgcg tctcgacggc
     2461 tcttgccgag atccttggcg cggccatagg cctcgacctg ctgtttgggc taccgctccg
     2521 tgttggcgcc gtcatggtga cggcggctgt cacctggctc ctgctgagca acggctaccg
     2581 gcggatcgag aaagtcatca tcgcattcgt atcactgata gggctgtcat ttctgtatga
     2641 gctgtcgctc gtacacatcc cctgggcaga ggtctcagca ggatgggtca cgccggccat
     2701 tccactgggt tccattcccg tcgtcatgag cgtcctcggc gccgtcgtca tgcctcataa
     2761 cctcttcctc cacagtgaga tcatccagag ccgccaactg aacagggacg gaacggacgt
     2821 catcgagaag cagcttcggt tcgagttcgt ggacaccctg aactccatgg tgatcggctg
     2881 ggccatcaac agcgccatgg tcatccttgc agcagccacc ttctttgccg ccagaacccc
     2941 tgtcacggag cttggacagg ccgaggccat gctgcggcct cttctgggca aagcggcagc
     3001 cgtcgtcttc ggcggcgcgc tcctcttggc cggcctgtcg tcttctgtga ccgcgggcat
     3061 ggcaggcgga agcattgtgg ccggcatgtc cggcgaaccc tacgacccgt cggacaacca
     3121 cacacggttg ggagtcctga tgacacttct cggaggtctg ctgatcgcta tcgtcatcac
     3181 caacccgttt cgcggcctga tcatcagcca gatggcgttg tcagttcagc tgccgtggac
     3241 gatcgtcctg cagatcatgc tcacgtcaaa cccgcgtgtg atgggcaggt acgtgaacac
     3301 gagccttgac cgtgccctcc tgtggacgac cgccatcgtc gtcaccggcc tcaatgtcct
     3361 cctcctggtc gacacgctgc gaaatctcca tcattagcgg ctcaggcagc agcacacatc
     3421 tacgcattct tgacaagact cacttgctcg ggtttaatgg acacggcaga agggaagggc
     3481 accccctatc tggccatccc gcactggatc atatgggaag aaaggagttg tagtgtgaca
     3541 aactcgacac cggatgcacg ctggagaagg cagatcatcg ttgtgctggc catcatgctg
     3601 gccacggcct cctcgattgt tctctgtccc gctcctgacg tcagcgcagg cacgtacaac
     3661 cctctcaagg ctgttcagta tgccaaccaa tggtggaaca agcgcaaccc aaaattccag
     3721 agctatggtt tcaatgactg cgccaactac gtttcgcaat gccttatcgc gggaggcctc
     3781 agtctgcggg cgagtccctc tgcggacaag agcggaagtc ttgcgcagtg caccagcctg
     3841 gacgcctacc tgacgaacac gctgaaagct actgtctgga agacttcgtc gagtgcgcgc
     3901 ccgccgaccg ccctgcaacc cggtgacgtc gcgatctttg gcaactggct aggcctgcgt
     3961 cacgccgtct tcgtctcggc caagcagaca aacggtcagc ccctgttcaa cgcccacaca
     4021 tatgatcgac tgcggaccac tatcgactgg ctgttcagcg cctggacata cgtcaaatac
     4081 taccacatcg tcaagtcgcc gatcactgtc gcggtcgctg ccaagccggc agccccggcg
     4141 aagacagcgg tggtggcaca gcctgccgtg acgacgagaa gttccatcgc tgaacccaag
     4201 cctgaaccgg tcgagaccgc ggcgctcgtc aagacagtcc ctgcctcatc ggtcacatcg
     4261 acctctgcgc tgctgcatgg catcgtcagg cccgatggtc agaccactct ctggttcttc
     4321 gagtatggcg tctcagagga ctacgacgag gccacggaca agcagggacc cctcctcggc
     4381 aacatgacct atgacgtcga ccttgaggtc gccgggctca aagagagcac gctgtaccac
     4441 gctcgtctgg tgcgggtcgc accggacggc agtctcacca ctgggtcaga catcacgttc
     4501 aagaccacgg aggctccgca ctccgtcacg cgccaggact gagcacgggg agtacttcaa
     4561 caccgtcttc gatacttggc ggctgttgga ggatccgctc ggctatcctc tggctacgaa
     4621 gaagactcgt ggttcagact cggggtcagc gagaggggca gtcagctccg ccgctcgcgc
     4681 ctggtagacg gcgctgaatc cgacttcaag cagcgccgtc gttacctctc gaaccgacca
     4741 cgcgttttcc acgaacgtct cgtcgaagcg gagccagctg cctgactcct gacggacgaa
     4801 cccggagatc ttgatccgtc cctgacgcat gtcttcgtcg tacatgccac ggttgatgac
     4861 catcgcatcc agggcgtcct cgaccgttac attgttccag cgtcgcagtc ccatcaccgt
     4921 attgagatca aacacgaaca ggccccccgg cagcagacac tgatgcacgc aggcaaagca
     4981 ggacagcaac gcgtcgatgc cggacaggtg gttgagggcg tcgaaggtcg agaccgcaaa
     5041 tccaaaccgc gaggacaacg agaagtcagc cgcgtccttc tggacaaacg aaacctcgcc
     5101 cgacgagacg aatgacgcac aattcatgcg cgcgtgagcc agcatgggct ccgagttgtc
     5161 cagtcccgtc acaacgtacc ctgcctgcag gaattgcagt gccagttggc cggtaccaca
     5221 gcagacgtcg agcagcgacc gctccccctg gggg
//
LOCUS       DATMZD010000012         2986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_80823,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000012 DATMZD010000000
VERSION     DATMZD010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80823"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1076)
                     /locus_tag="VN478_00215"
     CDS             complement(<1..1076)
                     /locus_tag="VN478_00215"
                     /inference="COORDINATES: protein motif:HMM:NF027579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4910 domain-containing protein"
                     /protein_id="HWQ21139.1"
                     /translation="MTLREDLLREFCPQNALELERDLALLHRSSGGEGFVDAIARLQE
                     FVGAGTVRHYPHTDACQGYLMPRPWNLKGGSLRLSTGEWLVPDLRNAPIGGVFLSGAS
                     RGVERLTVVDVGSGTEDPDYAQPVEGCAVLATGKPPVVYREAVMSRGARCVISDFMPV
                     QDARIGRTPDEMPDAVSYTSFGAESGGEGFGFNVSSHTFKRLQDMAGKETVQVDAFFD
                     ADAGTGDLQLLEAHAGTPGARKPLLLQAHLCHPRPGANDNASGAALLAELVRVLRKVP
                     LEREVIALWMPEFYGTIAWLADQKPDLACAIDLDMVGEDQAETCSTLEVTATPWSLPS
                     FIPNLMAVNLASHDFRLMLTGFTWG"
     gene            complement(1073..1645)
                     /locus_tag="VN478_00220"
     CDS             complement(1073..1645)
                     /locus_tag="VN478_00220"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWQ21140.1"
                     /translation="MEILPVIMTGTKVVLTPACREDVPAYLHWITNPELNVFLLDYGK
                     VCTLEQEYAWYDDVVAKAASHHMVHMDIRLTEDQKLIGDCSLFDINSREGTAEVGILV
                     GEKDYHSKGYGSEALYLLCRYGFDKLNLHSILLRHLSINARGDKAYRKIGFREFGCFR
                     ESVVRDRVRYDMVYMDLLEPELLNPVAGGR"
     gene            1901..2116
                     /locus_tag="VN478_00225"
     CDS             1901..2116
                     /locus_tag="VN478_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015589931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HWQ21141.1"
                     /translation="MDTTKCFNEVDPVCGMVLNSCEAVETSVFEGKTYGFCSKRCKEF
                     FEGDPKKYIENPASRKSMPAPKEVPEK"
     gene            2292..>2986
                     /locus_tag="VN478_00230"
     CDS             2292..>2986
                     /locus_tag="VN478_00230"
                     /inference="COORDINATES: protein motif:HMM:NF016816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HWQ21142.1"
                     /translation="MKYFISLDMEGVAGLASWSETDEEQRELMTGETCAAIEGIRSVD
                     SAAAILVADSHSRGQNLILDKLPQDIQVVRGYPRPMYMVEGADTSFDAALFVGYHAPI
                     GTKNGSMDHTYSSSAIYEVLANGHVVGETELNMVVLSRMGIPLIFCSGDDQYCAFSQA
                     YFPGATFVTTKWGMGRYSQRTLMPEASRNLIREGAAAATRTLIAKGRAALAAAQTTGT
                     LQQEVLTATPSTWT"
BASE COUNT          605 a          889 c          940 g          552 t
ORIGIN      
        1 ccccaggtga acccggtcag catcaggcga aaatcgtgcg acgccaggtt cactgccatc
       61 aggtttggga tgaacgacgg cagtgaccat ggcgttgccg tgacctccag ggtggagcag
      121 gtctctgcct ggtcttcgcc gaccatgtcc aggtcgatgg cgcatgccag atcgggtttc
      181 tgatcggcca gccatgcgat cgtaccatag aattcgggca tccagagtgc gatgacctcc
      241 cgctccagcg ggaccttgcg caggacgcgg acaagttccg caagcagggc ggccccactg
      301 gcgttgtcgt ttgcccccgg ccgggggtgg cacaggtgcg cttgcaggag aagcggcttg
      361 cgggctcccg gagtgccggc atgagcttcc agcagctgaa ggtcgcccgt gccggcgtca
      421 gcatcgaaga aggcgtcgac ctggactgtc tccttccctg ccatatcctg caggcgcttg
      481 aacgtgtgac tgctgacgtt gaagccgaag ccttcacccc cggactctgc gccgaatgag
      541 gtgtagctca cggcgtcagg catctcgtcg ggcgtccgcc cgatacgggc gtcctgcacc
      601 ggcatgaagt ccgagatgac gcagcgtgcc ccgcggctca tgaccgcttc acggtatacg
      661 acaggaggct tgcccgtcgc cagaacggca cagccttcga ctggctgagc atagtcggga
      721 tcctccgtgc cggacccgac gtcgacgacg gtaaggcgtt ccacgccgcg actggccccc
      781 gagaggaaga caccgccgat cggggcgttc ctcaggtcag ggaccagcca ctcgccggtg
      841 gacaggcgca gcgatccccc cttgaggttc catgggcgtg gcatgagata tccctgacac
      901 gcatccgtgt gcggatagtg acgcaccgtg cctgctccca cgaattcctg cagtctggcg
      961 atggcgtcga caaacccttc tccacccgac gaacgatgca ggagtgcaag gtcgcgctcc
     1021 agctccagcg cattctgtgg acagaattcc cgaagcaggt cttcccgcag cgtcatcttc
     1081 ctccagcaac tggattgagc agctcaggct ccaacaggtc catatagacc atgtcgtagc
     1141 gcacgcggtc gcggacgacg gactcgcgga agcagccgaa ttcgcggaag ccgatcttgc
     1201 ggtatgcctt gtcgccgcgt gcattgatgg acaggtgccg caggaggatg ctgtgcagat
     1261 tgagcttgtc gaacccgtag cggcacagga ggtagagcgc ctcgctgccg taccccttgc
     1321 tgtggtaatc cttctcgccg acgagaatgc cgacctcggc ggtgccttcc ctgctgttga
     1381 tatcgaacag agagcagtcg ccgatgagct tctgatcctc cgtcagccgg atgtccatat
     1441 ggaccatgtg gtggctggcg gccttggcga cgacgtcgtc gtaccatgcg tactcctgct
     1501 ccagggtgca caccttgccg tagtcgagca ggaagacgtt cagttcaggg ttcgtgatcc
     1561 agtgcaggta tgccgggacg tcctcgcggc acgcgggtgt caggacgacc ttggttccag
     1621 tcatgatgac aggcaggatc tccatgttcc aacctcctca aaatcgatga aaccagcata
     1681 cccacaaggg cgctgtggtg caagggttca gggtgacccc atcggacggg cccatgttgt
     1741 acgtttgtgg cggtttggcg aaaagacagc gtcccgatag tcttgcattg gagcatactt
     1801 ctccctagag taatgttaga gagaagacca aagcagccga ggagacctcg gattccgata
     1861 attcatgcgt gacgacagac cattggccga ggaggctggg atggacacga caaagtgttt
     1921 caacgaagta gatcccgtat gcggaatggt cctcaattcc tgtgaggcgg tcgaaacgtc
     1981 ggtattcgaa ggcaagacct acgggttctg cagcaagcgg tgcaaggagt tcttcgaagg
     2041 cgatccgaag aagtacatcg agaacccggc ttcgcgcaag agcatgccgg cacccaaaga
     2101 ggttcctgag aagtagagat cagttgcgac agtgttggat agcacgacgg ccgcgagttc
     2161 gcggccgtcg tgcatgtcac gggacatagc cacttcgcgc gggcaacggg caaggcgcaa
     2221 caaggctata cttgaatcgc tcatgagaag actcagatta ctgcggcggt agccgccggg
     2281 aggccaccgt aatgaagtac ttcatcagtc tagacatgga aggcgtggca gggctggcat
     2341 cgtggtcgga gacggatgag gaacagcgcg agctcatgac cggagagacg tgcgccgcca
     2401 tcgagggcat tcgcagcgtc gacagtgcgg cagccatcct tgtcgccgac agtcacagcc
     2461 gcggacagaa cctgatcctc gacaagctgc cccaggacat ccaggttgtg cggggctacc
     2521 cgcgtccgat gtacatggtc gagggtgccg acacaagttt cgatgcggca ctgttcgtcg
     2581 gctaccacgc gccgatcgga acaaagaatg gttccatgga tcacacctac tcgtcgtcgg
     2641 ccatctatga ggtcctggcg aacggacacg tcgtgggtga gacggagttg aacatggtcg
     2701 tgctttcccg catgggcatt cccctcatct tctgcagtgg cgatgaccag tactgcgcgt
     2761 tctcacaggc gtacttcccc ggcgccacct tcgtgacgac caaatggggt atgggacggt
     2821 attcccagcg aacgctgatg cccgaagcat cgaggaactt gattcgcgag ggggccgccg
     2881 ccgcgacccg cacgctcatc gcgaagggcc gtgcggcact ggcagcggcg cagaccaccg
     2941 ggaccctgca gcaggaggta ctgacggcga cgccatctac ctggac
//
LOCUS       DATMZD010000013         2424 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_453,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000013 DATMZD010000000
VERSION     DATMZD010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1099
                     /gene="ispF"
                     /locus_tag="VN478_00235"
     CDS             <1..1099
                     /gene="ispF"
                     /locus_tag="VN478_00235"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HWQ21143.1"
                     /translation="ICTTLEAVCQAPVRRLVLVCRVEDRAKMEKCVVQAALPRRVRVL
                     MADGGPTRTDSVLSGLRTLLADGSSDRDWVLIHDGARPLVSRALLKRLLAAGDDSDIV
                     VPVTPVIDSLRSVSGGTTEALDRGGVVCVQTPQLCRLATLGSAYLGSVRNVSFGDDAS
                     LVEAAGGRVSVVEGDVHNVKVTVPGDVWAIETALTAHSRTVVGFGYDVHRFVTGRPLV
                     LGGVRIASDYGLEGTSDADAVLHAVMDAILGCAGAGDIGSMFPSSEGQFAGIDSSVLL
                     RRVLSDRHVHRLRILSADITVVAERPRLQDYQAPMRRRIAHLLGLRSSNVDVKVTGND
                     AIGWIGRGEGIASLCVVTALRLEWHKERTVR"
     gene            complement(1089..1353)
                     /gene="ffs"
                     /locus_tag="VN478_00240"
     ncRNA           complement(1089..1353)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VN478_00240"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(1397..>2424)
                     /locus_tag="VN478_00245"
     CDS             complement(1397..>2424)
                     /locus_tag="VN478_00245"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HWQ21144.1"
                     /translation="PDRIDELLGSLRESRTTQAPASVSVEPAPKHHRRSKIWIVFALV
                     AVVATAAIFLFFQGRKASLNIRSFPSGATVLLDTRQVGITPLVLSGVAAGTHAIEIHL
                     AGWDVWKGSATAVRGSTTQVIANLTHAAYALVVTSTPAGATVLLDGTAKGVTPLTIVG
                     LKPRNYAVVVSLKGYAAISRTVDLSDPTQAAQDFSLVQAFGKLSITSDPTGAQVFLDG
                     KAYGVTPLKIDPFPVGNYSLTLKLEGSKDVTDVLSVKDGVPLSKQYKFDLALGGLSIT
                     TDPAGASITIDGQATGQIAPFTFSKLKEGTHDIYLELPGYLPWSGDVTLASGQTSRLS
                     IALTKLH"
BASE COUNT          439 a          682 c          769 g          534 t
ORIGIN      
        1 catctgcact actctggaag cagtgtgcca ggcccccgtg cggcgtctgg ttcttgtctg
       61 cagagtcgag gaccgggcga agatggagaa gtgtgtcgtc caggcagctc ttccgcgtcg
      121 ggtgagggtc ctcatggcgg acggaggtcc aacccggaca gacagtgtcc tcagtgggct
      181 caggacactc ctggcagacg gctcttctga ccgggactgg gtgctgatcc atgacggcgc
      241 gcgcccgctg gtcagccggg cgttgttgaa gagactgctt gctgcaggag atgactccga
      301 tattgtcgtg cctgtaacgc ccgtgattga ctcgctgcgt agtgtatcgg gcggaacgac
      361 tgaggcactg gaccgcggag gagttgtctg tgtccagacg cctcagctct gccgactggc
      421 gacgcttgga tccgcatatc tcggcagcgt taggaacgtg tcttttggag atgatgcctc
      481 gctggttgag gcagccggtg gacgcgtctc tgttgttgag ggcgatgtgc acaacgtgaa
      541 agtgactgtg ccaggggacg tatgggcgat cgagacggca ttgaccgcgc actcgaggac
      601 agtagtgggg tttggctacg acgtacatcg gtttgtgact ggccgaccgc tggttctggg
      661 cggtgttcgg atcgcgtcgg actatgggct cgaagggacg tcggacgctg acgccgttct
      721 ccacgccgtc atggacgcca tcctcggatg cgcgggggcg ggcgatatcg gcagcatgtt
      781 cccatcttca gaagggcaat tcgccgggat cgacagctcc gtgcttctgc gacgcgtgtt
      841 gtccgacagg cacgttcaca ggcttcgtat tctctccgcg gacatcactg ttgtcgctga
      901 gagacccaga ttgcaggact accaggctcc gatgcggagg aggatcgcac atttgcttgg
      961 actgcggtcg tccaacgttg atgtcaaagt gacgggcaac gatgcgatcg ggtggatcgg
     1021 cagaggagaa ggcatagcct cgctctgcgt tgtgaccgcc ctgcgtcttg agtggcacaa
     1081 ggagagaacc gtacgctgag cacttgttac acaagtcccc ggcgtggtcg cctgtctccg
     1141 gctccagttg catcgagcca cggcacatgt tccactcagt actgttgctt ccttccggac
     1201 ctggcagagt tcagagcttc ccattgcatg gtttgcagcc ttcaacgcct tcaacaggga
     1261 ctgttttgga aatgtgcgtc ccgcatgccc agcatggacc ccggtaagcg gattccgggc
     1321 aacagggcac cgctagctcc ccatcctgta cggcttcagt agtatatagc ccgagatgtt
     1381 ccccgtcaac ccgctcttag tgcagcttcg tgagggcgat agagaggcgg cttgtttgcc
     1441 cacttgccag tgtcacgtcg cctgaccatg ggaggtatcc cggtagttcc aggtagatgt
     1501 catgcgttcc ctccttcagt ttgctgaacg tgaagggagc gatctgaccc gtggcttgcc
     1561 cgtcgatcgt gatggacgct cctgcggggt cagtcgttat ggagagcccg cccagcgcaa
     1621 gatcgaattt gtactgcttt gacaagggga caccatcttt cacggagagc acatccgtga
     1681 catccttgct gccctccagt ttgagcgtca acgagtagtt gcctacagga aacgggtcga
     1741 tcttcaatgg tgtcacaccg tacgccttgc catccagaaa gacttgagcc cctgtcggat
     1801 ccgacgtgat gctcaacttt ccgaacgcct ggacaaggga gaagtcctgt gctgcctgcg
     1861 tcggatcaga caggtcgacc gtgcgcgaga ttgccgcata gcctttcagg gagaccacga
     1921 ccgcgtaatt gcgtggtttg agtccaacta tagtaagagg cgtgactccc ttggctgttc
     1981 cgtcgagaag caccgttgca ccggctggcg tcgatgtcac gacgagagcg tatgctgcat
     2041 gtgtcaggtt ggctatgact tgtgtcgtgg accccctcac ggccgtcgcg gagcccttcc
     2101 agacgtccca gccggcaagg tggatctcta tggcatgggt tcctgcggct actccggaca
     2161 gcacgagcgg cgtgatgcca acctgtcgtg tgtcgaggag cacggttgct cctgacggga
     2221 acgaccggat gttcagactt gcttttcgcc cctggaagaa caggaagatg gccgcagtcg
     2281 cgaccaccgc caccagtgca aacacgatcc agatcttgct tcggcggtgg tgcttcggtg
     2341 ccggttctac ggaaacgctc gcaggagctt gtgtcgtgcg tgactcccgc aggcttccga
     2401 gaagctcgtc gatgcggtcg ggtt
//
LOCUS       DATMZD010000014         2715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_121069,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000014 DATMZD010000000
VERSION     DATMZD010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121069"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1032
                     /locus_tag="VN478_00250"
     CDS             <1..1032
                     /locus_tag="VN478_00250"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HWQ21145.1"
                     /translation="ADGTLYTVVDEPGSVGTFTMDVRQEKMVYFLGTMTDPGQVWVRE
                     METGSTRQLTHLNQKLLGELDLGKVEDVWFKARDGHDLQGWIMTPPGFDPKKKYPSIL
                     EIHGGPQDQYGYLFMHEFYFLAARGYVVYFSNPRGGTGYGEEHEKAIWGRWGTVDFDD
                     LMDWTDLVEKKPYIDHERMGVTGGSYGGYMTNWIIGHTHRFAAAATQRSVSNLISMWG
                     TSDFNWTFQLPHGGKTPQDSIEVLWDRSPVKYLGNATTPTMVLHNEMDFRCAIEQGQQ
                     VFTALKVHHVPAEFVTFPEEPHGLSRVGRTDRRIDRLESITRWMDTYLKPAATKAVKG
                     VGTSKKAPK"
     gene            1103..1882
                     /gene="trmD"
                     /locus_tag="VN478_00255"
     CDS             1103..1882
                     /gene="trmD"
                     /locus_tag="VN478_00255"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013251742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HWQ21146.1"
                     /translation="MASPCICYTVSTMRIKAISIFPQLYEPFTQVGVMKMAIDKGILD
                     FEAVDLRAYTHDRHHTTDDIPFGGGAGMMFLPRPLMEALDTIDDPAGSWLKIGVAPQG
                     DRLTQHMVEDLAQEERLLFVAGRYEGIDERVMERLDLTISIGDVVLSGGEIAAVEIID
                     AITRLLPGATGNAASTGEESFSSGLLEYPQYTRPRELAGEVVPDVLVSGHHANIARWR
                     REEALRRTLIRRPDLLRDFDLTDQDRKYLSKVLADLRGILD"
     gene            1875..2441
                     /locus_tag="VN478_00260"
     CDS             1875..2441
                     /locus_tag="VN478_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007786334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HWQ21147.1"
                     /translation="MIDFRLYLAVVHHPVYNKHHEIVTTSIVIHDIHDIARAGKTYGA
                     KAMYEVEPLPEEQQIALRIAHFWNEGFGHEYNPNRAESLSLLRIVSHFEEAVAEIQHI
                     EGERPVLIATSARTFANSIGYPAMGERIRSADHPYLLVFGTGFGLADEIMEQMNYVLD
                     PIWGPTEFNHLSVRSAAAIILDRLLGRS"
     gene            2443..>2715
                     /locus_tag="VN478_00265"
     CDS             2443..>2715
                     /locus_tag="VN478_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HWQ21148.1"
                     /translation="MSVGTAVKRVRREVRVWSLVLKDPRTPRVSKWLLGAALAYLASP
                     VDVIPDFIPLLGQLDDLVIVPGLVRLATRFIPHGVVAECRAKARPQD"
BASE COUNT          567 a          794 c          839 g          515 t
ORIGIN      
        1 gcggacggga cgctgtacac ggtcgtcgac gagccgggca gcgtcggtac cttcacgatg
       61 gatgtcagac aggagaagat ggtgtacttc ttgggcacga tgaccgaccc gggacaggtg
      121 tgggtgcggg agatggaaac agggtcgaca cgccagctca cgcatctgaa ccagaagctg
      181 ctgggagaac ttgacctcgg caaggtcgag gacgtctggt tcaaggcccg tgacgggcat
      241 gatctccagg gatggatcat gacgccgccc ggctttgacc cgaagaagaa gtatccgtcg
      301 atcctcgaga tccacggggg cccgcaggac cagtatggtt acctcttcat gcacgaattc
      361 tacttcctcg ctgccagggg ctatgttgtc tacttcagca atccgcgcgg cgggaccggg
      421 tatggcgaag agcacgagaa ggccatctgg ggtcggtggg gcacggtgga tttcgacgac
      481 ctcatggact ggactgatct cgtagagaag aagccataca tcgaccacga gcgtatgggc
      541 gtcacgggtg gcagctacgg cggctacatg accaactgga tcatcggtca cacccatcgt
      601 tttgctgctg cagcgacgca gaggagtgtc agcaacctca tcagcatgtg gggaaccagc
      661 gatttcaact ggacgttcca gcttccgcac ggtggaaaga cacctcagga cagcatcgaa
      721 gtcctgtggg acagatcccc ggtgaagtac cttggcaacg cgacgacgcc gacgatggtc
      781 ctgcacaacg aaatggactt tcgctgtgcc atcgaacagg gtcagcaggt gttcaccgcg
      841 ctcaaggtcc accacgttcc ggccgagttc gtcacgttcc ctgaggaacc gcatgggctg
      901 tcacgggtcg gacgcacgga ccggcgcatc gaccggctgg agagcatcac cagatggatg
      961 gacacctacc tgaaaccggc tgcgacgaag gcggtcaagg gcgtcgggac ttccaagaaa
     1021 gcacccaagt agctcacttt ccgtcactca tggacgcccc ggacagcacc ggggcgtttt
     1081 ccatgtcacg tggagttcac gcttggcgag cccatgcatc tgctatactg tctccactat
     1141 gcggatcaaa gcgatttcca tatttcccca gctgtacgaa ccgttcacgc aggtcggcgt
     1201 catgaagatg gccatcgaca agggtattct ggatttcgag gctgtcgacc ttcgcgccta
     1261 tacccatgat cgtcaccaca cgacggacga cattcctttc ggcggagggg cggggatgat
     1321 gttcctgccc aggccgctca tggaggcact ggacaccatc gacgatccgg cgggctcatg
     1381 gctcaagatc ggcgttgcgc cgcagggcga ccgcctgaca cagcacatgg tggaggacct
     1441 ggcacaggag gagcgactgc tgtttgtcgc ggggcgctac gaaggtatcg atgagcgtgt
     1501 catggaacgg ctcgatctca cgatctcgat cggagacgtt gtgctctcag ggggcgagat
     1561 agcggccgtc gagatcattg acgccattac ccgtttgctt ccgggggcca caggaaatgc
     1621 ggcgtccacg ggcgaagaga gcttctcgtc cggactcctg gagtatcccc agtacacacg
     1681 ccctcgcgaa ctggctggtg aggtagtacc cgacgtcctc gtttcggggc atcacgcgaa
     1741 tatcgccagg tggcgtcgag aggaggccct gaggaggacc ctcatccgca ggcccgacct
     1801 tctgcgggac tttgacctga ccgaccagga ccgcaagtat ctgagcaagg tgctggcaga
     1861 cctgaggggg atccttgatt gacttcagac tgtaccttgc cgttgtgcac cacccggtct
     1921 acaacaagca tcacgagatc gtcacgacgt ctatcgtcat ccacgacatc cacgacatcg
     1981 cacgagccgg caagacctac ggagccaagg cgatgtacga agtcgaaccc ctgcctgagg
     2041 agcagcagat tgccctgcgg atcgcccact tctggaacga agggtttgga cacgagtaca
     2101 acccaaaccg ggccgaatcg ctgtcactgc tgcggatcgt gtcacatttc gaggaagctg
     2161 tcgccgagat tcagcacatc gaaggtgaga ggccagtcct gatagcgaca agtgcacgga
     2221 cctttgccaa ttccatcggc tatccagcga tgggcgagag gattcgctct gctgaccatc
     2281 cgtatctgct tgtcttcgga acgggttttg gtctggctga cgagatcatg gaacagatga
     2341 actacgtcct ggaccccatt tggggaccga ccgagttcaa tcacctgtca gtgcgcagcg
     2401 ccgccgccat catcctggac cgcctgctgg gacgctcctg agatgagcgt aggaacggca
     2461 gtcaagcgcg tcaggcgcga agtccgtgtg tggtctcttg tgttgaagga cccgaggact
     2521 ccacgcgtat caaagtggct tctcggcgcg gcgctcgcct atctggcttc accagtggac
     2581 gtgatccccg acttcatacc gctactgggg cagctcgacg acctggtgat cgtgcctggc
     2641 ctcgtccggc tggccacgcg gtttatcccg catggcgtcg tggcggaatg cagagcgaag
     2701 gcccggcctc aggac
//
LOCUS       DATMZD010000015         3680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_462,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000015 DATMZD010000000
VERSION     DATMZD010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="VN478_00270"
     CDS             complement(<1..757)
                     /locus_tag="VN478_00270"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HWQ21149.1"
                     /translation="MQIVVTILAFVFSFTLATLFHEGGHMLAAAASHTQTEEFGLGFG
                     RTVWSRTWHGTVFKINALPILAYVKIKGMESNYDAPDGFYTKGWWARFFTMVGGMLGN
                     LVLAFIIFSIVFSTLGDPRASTKVGTVTPSSLAEAAGLKAGDNILSIDGVAMKDFDQI
                     SAVVRASQNRQIDVLVDRGGSRVDVFVTPAMDTKLGYVAIGYGPGFVRYNPFKAVWRA
                     LTFTGEYIVTYLKALPLLFTKRGVQSLTGPIGIA"
     gene            complement(761..1951)
                     /locus_tag="VN478_00275"
     CDS             complement(761..1951)
                     /locus_tag="VN478_00275"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:NF014700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HWQ21150.1"
                     /translation="MTGSKGLVLFGSTGSIGTQVLDVVCAQSERYHVVGLTAHSNDEL
                     LAEQTLTFQPRFVAMSDDAARERLVIRLERHDVRIPVVGDEDLPSMLSRVAFDMPVVA
                     AASTDLAQVVYDLLRLGMPMAIASKELLIMLGDLLEPFRTQLRPVDSELSAIWQCLAG
                     EDLESVERVILTASGGPFRDTAAAALGDVTAAQALQHPSWRMGRKVTIDSATMFNKAL
                     ELAETHVLFGLERERITAVQHPQSVVHGLVEFYDGTTRAIAYRPDMRVAIAYALSAPD
                     RPLREQAVRLHLEELGQLTFEPVDAKFEAFAVGREADMRSPRSMLALLGADEVAVSRF
                     LVAQGTFGDIVAALRHVLGETGDEPLGPSVRARIEAVSRGRQLAEEWYEKRYGTQPGD
                     REGV"
     gene            complement(1948..2652)
                     /gene="uppS"
                     /locus_tag="VN478_00280"
     CDS             complement(1948..2652)
                     /gene="uppS"
                     /locus_tag="VN478_00280"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005684322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HWQ21151.1"
                     /translation="MTTESQLHVAIVMDGNGRWAVGRGLPRVAGHRQGAQVVHDIVAA
                     APGLGVTALTIFAFSTENWKRPIDEVGYILHLFVAMVTKWLPELIAQHVRVRIIGDRA
                     ALPADVIASVETIEHRTASCDGLHLNVALNYGGRAEILHAARSFAAECADGRAVPEDL
                     SEDRFEAHMWLGEEVSPDIVIRTGAESRVSNFLLWEMAYAELFFLDVLWPDFTPATLE
                     MVVQKFKSTNRRFGGS"
     gene            complement(2667..3221)
                     /gene="frr"
                     /locus_tag="VN478_00285"
     CDS             complement(2667..3221)
                     /gene="frr"
                     /locus_tag="VN478_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HWQ21152.1"
                     /translation="MDKYFTPIDVKMQAAFQALLKDYSEIRAGRITPAYVDGVVVEAY
                     GQRNPLKDVATISAPQAKVLVVEPWDKNLLKEVERAILKANIGVTPTNDGQRVRLVFP
                     DLTLDERKKMVARISQLTEKFREEIRSIRREVRDDIKAKEKAKELSEDEQHRLEEELD
                     KLTDKHVAEVNKAFEAKEKEILSL"
     gene            complement(3222..>3680)
                     /gene="pyrH"
                     /locus_tag="VN478_00290"
     CDS             complement(3222..>3680)
                     /gene="pyrH"
                     /locus_tag="VN478_00290"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Catalyzes the phosphorylation of UMP to UDP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HWQ21153.1"
                     /translation="LQSAFEQVGLEARAMSALRLDQVCEPYIRQRALEHLRRNRVVIF
                     AGGTGLPYFTTDTVAALRAVEVGADIVLKGTNVDAVYSSDPRTDKTAAPYSQLSYDQF
                     LAGHLKAMDATAVSLCRENHLPILLFNMNKPGNIVRAVMQGDIGTLIKEA"
BASE COUNT          772 a         1147 c         1026 g          735 t
ORIGIN      
        1 gggcaatgcc gatgggcccg gtcagcgatt ggacgcctcg tttcgtgaac agcaatggca
       61 gtgccttcag gtacgtgacg atatactctc cggtgaacgt cagcgcccgc cagacggcct
      121 tgaaggggtt gtagcggacg aatcccggac cgtagccgat tgcaacgtag ccaagcttcg
      181 tatccatggc aggggtcaca aacacatcga cccgtgatcc gccccgatcg acgagaacgt
      241 cgatctgtct gttctggctg gctctgacga cggccgagat ctggtcaaag tccttcatgg
      301 cgacgccgtc gatggagagt atgttgtccc ctgccttgag gccagcagct tctgcaagag
      361 aggaaggagt gaccgtgccg accttggtgg acgcacgcgg gtctccaagt gtcgagaaca
      421 cgatgctgaa gatgatgaat gccaggacca ggttgccaag catgccgccg accatcgtga
      481 agaagcgcgc ccaccagcct ttggtgtaga agccatcggg cgcatcgtag ttggattcca
      541 tacccttgat cttcacatag gcaagaatag gaagggcgtt gatcttgaag actgttccat
      601 gccatgtacg tgaccatacg gtgcggccaa atccaagtcc aaattcctcc gtctgggtat
      661 gagaagcggc ggcggcaagc atatggccgc cctcatggaa gagggtcgcc agcgtaaagc
      721 taaacacaaa cgcaaggatc gtaacgacta tttgcacagg tcagactcct tcccggtcgc
      781 ccggttgagt gccatatcgc ttttcatacc actcttcagc cagctgacgc ccgcggctca
      841 cggcttcgat acgtgcacgg acgctcggtc caaggggctc atcgccggtc tcgcccagca
      901 catgtcgcag ggctgccact atatcgccaa acgtgccctg tgcaacgagg aagcgtgaaa
      961 ccgccacctc atctgcgcca agcagcgcga gcatcgaccg cggagatcgc atgtccgctt
     1021 cgcggccgac ggcaaacgct tcaaacttcg cgtcgacagg ttcgaacgtg agctgtccaa
     1081 gttcctccag gtgcagccgg accgcctgct cacgcagggg acggtcagga gccgacaacg
     1141 catatgcaat ggcgacgcgc atgtcagggc ggtacgcgat ggcacgcgtg gtgccgtcgt
     1201 agaactcgac caggccgtgg accacagact gagggtgctg tactgccgta atccgctcgc
     1261 gctccaggcc aaacaacacg tgtgtctcag ccagttcaag cgccttgttg aacatcgttg
     1321 cagagtcgat cgtcaccttg cgccccatcc gccaagaagg gtgctgcagt gcctgagccg
     1381 ccgtcacgtc acccagcgcc gcagcagccg tatctcgaaa cggaccacct gatgcagtca
     1441 ggatgacgcg ctcgacgctc tcgaggtcct cgcccgccag gcactgccag attgccgaca
     1501 gctcgctgtc gactgggcgc agctgcgtcc tgaacggttc cagcagatct cccaacatga
     1561 tgagcagttc cttgctggcg atagccatgg gcatgcccag tcgcagcagg tcgtagacga
     1621 cctgcgccaa gtcggtcgag gccgccgcga ccaccggcat gtcaaaagcc accctgctga
     1681 gcatcgaggg tagatcctca tcgccaacga ctgggatgcg cacgtcatga cgctcaagcc
     1741 tgatcaccaa ccgctcgcgt gcagcatcgt ccgacatcgc cacgaaacgt ggctgaaagg
     1801 tcagggtctg ctcggcgagc agctcgtcat tgctgtgcgc cgtgagacct acgacgtggt
     1861 atcgctcaga ttgagcacac acgacgtcca gtacctgcgt tccaatagat ccagtcgacc
     1921 cgaagagcac gagacccttg ctacctgtca tgagccacca aacctccggt tcgtggattt
     1981 gaacttctgc acgaccatct caagtgtcgc tggcgtgaaa tccggccaga gcacatcgag
     2041 gaagaacagc tcggcatagg ccatctccca tagcaggaag ttcgagaccc gtgactccgc
     2101 tccggtgcgg atcacaatat ccggcgaaac ctcttcccca agccacatgt gcgcctcaaa
     2161 cctgtcttcg ctcaggtcct ccggcactgc ccgaccatcc gcacactctg ctgcaaatga
     2221 ccttgccgca tgcaggatct ccgctcttcc tccgtagttc agggctacgt tgaggtgaag
     2281 gccgtcgcac gatgcagtgc ggtgctcaat cgtctcaacg gaagcgatga cgtcagcggg
     2341 cagagcggca cgatctccga tgatcctcac tcgaacgtgc tgagcaatca attctggcaa
     2401 ccacttcgta accatcgcca caaacaggtg gaggatgtag cccacttcgt cgatcggccg
     2461 cttccagttc tctgttgaaa acgcaaagat ggttaaggcg gtcacaccca acccaggcgc
     2521 ggccgccacg atgtcatgta ctacctgggc gccctgtctg tgaccggcga ctctagggag
     2581 tccccgtccg actgcccacc tgccattgcc gtccatgaca atggcgacat gaagctgact
     2641 ttcagtggtc acgacgtcag aagtccctac agagacagga tctccttttc cttggcctcg
     2701 aacgctttgt tgacttctgc gacgtgcttg tccgtcaact tgtcgagttc ctcctcgagc
     2761 cgatgctgtt cgtcctcgga gagctccttg gccttctcct tcgctttgat gtcatcacgg
     2821 acctcgcgtc gaatggatcg gatctcctca cggaacttct ccgtgagctg ggagatgcgt
     2881 gccaccatct tcttcctctc gtcgagagtc agatcaggga agacgagccg cacccgctgg
     2941 ccgtcattcg tgggcgtgac gccgatgttg gccttcagga tggcacgctc gacttccttc
     3001 agcaggttct tgtcccacgg ctccacgacc agaaccttcg cttggggggc ggaaatcgtc
     3061 gcaacatcct taagaggatt gcgctgtccg taggcttcga cgaccactcc atcgacatag
     3121 gcgggcgtga tccggccggc acgaatctcg gagtagtcct tgaggagagc ctgaaaagca
     3181 gcctgcatct tgacatcgat tggagtgaag tacttgtcca cctaagcctc cttgatcaga
     3241 gtcccaatgt cgccttgcat caccgcccgg acgatgttac cgggcttgtt catgttgaac
     3301 agcaagattg gaagatgatt ctcacggcac agcgagacag ctgtcgcgtc catcgccttc
     3361 aggtgtcctg ccagaaactg gtcgtaactc agctggctat acggtgcggc cgtcttgtcg
     3421 gtgcgtggat cgctggagta gacagcgtcc acgttcgtac ccttcaggac gatgtcggct
     3481 ccgacttcga cagctcgaag cgctgccacc gtatcggtcg tgaaatatgg aagaccagtc
     3541 cctcccgcaa agatgaccac gcggttcctg cgaaggtgct caagagcgcg ctgacggatg
     3601 tatggttcac aaacctgatc caagcgcaac gccgacatcg cccttgcctc gagtcccacc
     3661 tgttcaaacg cagattgcag
//
LOCUS       DATMZD010000016         4533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_121103,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000016 DATMZD010000000
VERSION     DATMZD010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4533
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121103"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1918)
                     /locus_tag="VN478_00295"
     CDS             complement(<1..1918)
                     /locus_tag="VN478_00295"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HWQ21154.1"
                     /translation="MSQASAVKKLACTVLMLMAAFMLSPVDGVGAAEAERIVSFDSVV
                     TVQADSTMTVQETIRFVSAGITIQHGLYRDFPTTYDNPGRAPVKVAFQVLSLMRDGAT
                     EPWHSVRQTNGIRVYFGSSSTDLDAGEHTYVFTYSSDRQLGFFADHDELFWNVTGNGW
                     EYSIEQASCTVILPGNSWQQISGLTAYTGPQGATGTAYTVSRDTGGNPVFTTTAPLGQ
                     FEGLSVVVGWPKGIVMPPSSLQTMRWWLRDNRAFGIAALATIGLLLYYMLTWLRVGRD
                     PKPGTIVPQFGPPAGLSPAAIRYLRVMGSDTKGLSAAITGLAVKGALVISQDEDGTFA
                     VDTTGSTPAGLLPDESELLQELFDGRAKTRLVFKQSAHSRIQAVRKSLEQALQSTYGK
                     GYFVTNVRYAATGIALSAAGVIAAGLLGTTQPERIFGFVFMSVWLSMWTFGVAALVAQ
                     VVASWRGQRSGVSGRRKFSLPTSGCLTLFAIPFLLGELFGTIAFVVIAGPLLLALVFV
                     TAAIDVVFFRLLRAYTPQGRALLDQIYGFRMYLSTAEKDRMNMLTPVDHTPETFERYL
                     PYALALDVEQQWSENFADVLERTDASGEPIYQPAWFSGDALRSTGFSTLGSSLSGAFA
                     SSIAASSVAPGTSSG"
     gene            complement(1929..2489)
                     /locus_tag="VN478_00300"
     CDS             complement(1929..2489)
                     /locus_tag="VN478_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HWQ21155.1"
                     /translation="MPWLIALGVIALIVVWLIGIYNQLVKKRTMKDEAWSGVDVQLKR
                     RYDLIPNLVETVQGYAGHEELVFKEVTDMRTRAMGATGVADKAAAENQLNGTLKTLFA
                     VAEAYPELKANANFLDLQAKLADLEDQIQMARRYYNGTVRDYNIMCETFPSVFVASMF
                     GFKKAEFFELNGAEERETPKVSFDNK"
     gene            complement(2593..3273)
                     /locus_tag="VN478_00305"
     CDS             complement(2593..3273)
                     /locus_tag="VN478_00305"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HWQ21156.1"
                     /translation="MPQASERVRRILAISCANIVDHRSTSASTRTCRLAAEEAARQSN
                     DARTDILALVDLDLHPCTMCGACLRDPAFHCVDEDFNTLLDATIAADAVVMVIPHYAG
                     LPSKLVMVMEKLQELAFLLGENGRQKEFPLRGKPVALVGHGGMVEDPDALEYYRKALL
                     GPVQQALGSIGMKIVPAGARQPWGVTFGITGMNRAPGTFVSEMEHDWDRISSQLRPLV
                     TSLLAVAR"
     gene            complement(3275..3637)
                     /locus_tag="VN478_00310"
     CDS             complement(3275..3637)
                     /locus_tag="VN478_00310"
                     /inference="COORDINATES: protein motif:HMM:NF017880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HWQ21157.1"
                     /translation="MIYHIETEELWQQAVPAGQYVPDSYESDGFIHCSALEQITDVAD
                     RHYHGQQGLVLLCISQKLLDAETLWEPSDGLYYPHVYGPLNTSAVVAALPFPCSSDGT
                     FQLPPDMPEDTDDFPEAE"
     gene            complement(3668..4165)
                     /locus_tag="VN478_00315"
     CDS             complement(3668..4165)
                     /locus_tag="VN478_00315"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HWQ21158.1"
                     /translation="METTARPMRRKECEVTDAGVIHRILGRAALCRVAMVDGDEPYLV
                     PMNCGWDGERLFLHAAAQGRKLDILRANPRVCVEIDEDIQIVTGATGEECTANYVTVI
                     GTGTASFVPDPESKGRDLNIIIHQCHPGVPEETFTDKTLSSVVVLEVHFDHLTCKAKG
                     TTPRL"
     gene            complement(4262..>4533)
                     /locus_tag="VN478_00320"
     CDS             complement(4262..>4533)
                     /locus_tag="VN478_00320"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HWQ21159.1"
                     /translation="GSIECEVAALDGTAVDVHTTNGNLTLRVAQDLPATLDASTTNGN
                     LDFAGITLDNATASKHSIRGDLGTPKGTLSARTTNGNLTVQKLNP"
BASE COUNT          860 a         1364 c         1421 g          888 t
ORIGIN      
        1 atcctgagct tgttcccggc gccactgatg aagcggcgat cgaggaggcg aaggctcccg
       61 aaagtgacga tccgagtgtc gagaagccgg ttgagcgcaa ggcgtcaccg gagaaccacg
      121 cgggctgata gattggctcg ccggacgcgt cagtgcgctc caggacgtca gcgaagtttt
      181 cgctccactg ctgctcgacg tcaagtgcca gcgcatatgg caggtagcgt tcgaacgtct
      241 ccggcgtgtg atcgacaggg gtgagcatgt tcatgcgatc tttctcggcc gtactgagat
      301 acatgcggaa tccatagatc tggtccagaa gagctcggcc ctgtggtgtg taggcccgca
      361 gaaggcggaa gaagacgacg tcgatcgcgg cggtcacgaa gaccagcgcc agaagcaggg
      421 ggcccgctat caccacgaac gcgatcgtgc cgaagagttc gcccagcagg aacggtatcg
      481 cgaacagcgt aaggcagccg gacgtcggaa ggctaaactt gcgtctcccc gagacacccg
      541 agcgctggcc gcgccacgag gcgacgacct gtgcgacgag tgccgccacg ccaaacgtcc
      601 acatcgacag ccagacggac atgaacacga acccgaagat acgctcgggc tgcgtcgtgc
      661 ccagcaggcc cgcagcgatg acgcccgcgg cggagagggc gatccccgtc gccgcatagc
      721 gcacgtttgt gacgaagtat cccttcccgt atgtcgactg caaagcctgc tcaagtgact
      781 tccgaacggc ctgaatgcgg ctgtgggcgc tttgcttgaa gacaagtcgt gtctttgctc
      841 gaccgtcgaa cagttcctgc aggagttcgg actcgtcggg gagcaggccg gccggtgttg
      901 agccggtggt gtcgacggcg aacgtgccgt cttcgtcctg tgagatgacg agcgcgccct
      961 tgacggccaa tcccgtaatg gcggccgaaa gtcctttggt gtcagacccc atgacccgca
     1021 ggtaccggat ggcggcggga ctgaggccgg ccggcggccc aaactgcggg acgatggttc
     1081 cgggcttcgg atcgcggccc acacgcagcc aggtgagcat gtagtacagc aggaggccaa
     1141 tggttgcaag ggcggcaatg ccaaaggccc tgttgtctcg cagccaccag cgcatcgtct
     1201 gcagggacga gggcggcatc acgatgccct tcggccagcc gacgacgacg ctcaaccctt
     1261 cgaactggcc gagcggagcc gtggtcgtaa acacgggatt gccgccggtg tcgcgagaca
     1321 cggtgtaggc agtgcctgta gcgccctggg gaccggtata ggcggtcagt ccactgatct
     1381 gctgccatga gttccctggc aggatgaccg tgcagctcgc ttgttcgatc gagtattccc
     1441 agccgtttcc cgtcacgttc cagaacagct cgtcatggtc ggcgaaaaac cccagctgac
     1501 gatccgacga gtaggtaaac acgtatgtgt gctcaccggc gtccaggtcc gtagaactgg
     1561 acccgaagta gacccggatg ccgtttgtct gccggacaga atgccatggt tcggtcgctc
     1621 cgtcccgcat gaggctgagg acctggaagg cgaccttgac gggagcacgc cccggattgt
     1681 cataggtagt ggggaagtca cggtacaggc cgtgctggat ggtgatcccg gcggagacga
     1741 accgaatcgt ctcctggaca gtcatcgtgg agtcggcctg gacagtaacg acactgtcaa
     1801 acgacacgat gcgctcagct tcggcagccc ccacaccgtc cacaggggaa agcatgaaag
     1861 ctgccatcag catgagaacc gtgcaggcga gcttcttcac ggccgacgcc tgactcatgg
     1921 acagactgct acttgttgtc gaacgagacc ttcggggttt cgcgttcctc tgcgccgttc
     1981 agctcgaaga actcggcctt cttgaagccg aacatgcttg cgacaaagac ggatgggaac
     2041 gtctcgcaca tgatgttgta atcgcgtact gtaccgttgt agtagcggcg agccatctgg
     2101 atctgatcct cgaggtccgc aagcttcgcc tgcagatcga ggaagttggc gttggccttg
     2161 agttcaggat aggcttccgc caccgcaaac agggtcttca gcgtcccgtt cagctggttc
     2221 tcggccgcag ccttgtcggc gacgccggtt gcccccatag cacgagtgcg catgtcggta
     2281 acctctttga agaccagctc ctcgtggccg gcatacccct ggactgtctc cacgagattc
     2341 gggatgaggt catagcggcg tttcagctgg acgtccacgc ccgaccatgc ctcgtccttc
     2401 atggtgcgtt tcttgacgag ctggttgtag atgccgatga gccatacgac aatgagagca
     2461 atgactccaa gagcgattag ccaaggcatt gtgttcctcc ttttgcagat agcggaaatg
     2521 cggcatcacc caatactgcg aggcgcctgt ccatagtgta gagaaaaccg cacatataca
     2581 aaagagtccc ttctaccggg ctacagccag cagtgacgtg acgagcggcc tcaactgaga
     2641 actgatccgg tcccagtcat gttccatctc cgatacgaac gtgcccggag cacggttcat
     2701 gcccgtgatg ccaaatgtga cgccccacgg ctgcctggcg ccggccggca cgatcttcat
     2761 gccgatactg ccgagtgcct gctgcacagg gcccagaagc gccttgcgat agtactccag
     2821 ggcgtcgggg tcctcgacca tgcctccgtg acccacaaga gcgaccggct tccccctcaa
     2881 ggggaactcc ttctgccttc cgttctcgcc aagcagaaac gccagttcct gcagcttctc
     2941 catgaccatg accagcttgg agggcaatcc tgcatagtga ggaatgacca tgacgacagc
     3001 atcggccgct atcgtagcat ccagaagggt gttgaagtct tcgtcgacgc agtggaacgc
     3061 ggggtcgcga aggcatgcac cgcacatggt gcaggggtgc aggtccaggt cgacgagcgc
     3121 caggatgtcc gtccgggcat cgttgctttg ccgggctgcc tcctcggctg ccaggcggca
     3181 tgtgcgggtg gatgcactgg tgctcctgtg gtcgacgatg ttggcgcagg agatcgcgag
     3241 gatcctccgg acgcgttctg atgcctgagg catcctactc cgcttccggg aagtcgtccg
     3301 tgtcctccgg catgtccggg ggcagctgga acgtgccgtc gctgctgcac gggaatggaa
     3361 gcgcagccac gacggcagac gtgttgagtg gaccgtagac gtgaggatag tagaggccgt
     3421 cggagggttc ccacagcgtc tccgcgtcaa gcagcttctg gctgatgcac aggagcacca
     3481 gaccctgctg gccgtggtaa tgcctgtctg cgacgtcagt gatctgttcc agcgctgaac
     3541 agtggatgaa gccgtcgctt tcgtagctgt cagggacgta ttgtcctgcc ggtactgcct
     3601 gctgccacaa ctcttcggtc tcgatatggt agatcatgcc ttctttcctc caatcacgaa
     3661 tgtggcgcta gagacggggc gtcgtcccct tcgctttgca cgtgaggtgg tcgaagtgga
     3721 cctccagaac gacgacgctc gacagcgtct tgtcggtgaa ggtctcttcg gggacacctg
     3781 gatgacactg atggatgatg atgttcagat ccctgccctt gctttcgggg tcaggcacga
     3841 atgacgcagt gccggtcccg atgacggtca cgtagttcgc agtgcattcc tcgccggtcg
     3901 ctcccgtgac gatctggatg tcctcgtcga tctcgacgca gacgcgcgga ttggcgcgca
     3961 ggatatccag cttcctgccc tgcgccgccg cgtggaggaa caagcgctct ccatcccagc
     4021 cacagttcat cgggaccagg tatggctcgt ccccgtccac catcgccacg cggcagagcg
     4081 cggcgcgtcc caggatgcga tggatcacgc cggcgtcggt cacctcgcac tccttccttc
     4141 tcatcggcct tgctgtcgtc tccatcgttc ctcccgggcc ctttgcgggc acgcgaacag
     4201 tatacggaaa accccgcggc cgtcacgggt cgcggggtcg tatgaacatg caggagttgt
     4261 ttcaggggtt gagcttctgc accgtgaggt tgccgttcgt cgtgcgggcc gacagcgtac
     4321 ccttgggagt tccaagatca cccctgatgc tgtgcttgga cgccgtggca ttgtcgagcg
     4381 taatacccgc gaagtccagg ttgccgttag tggtcgatgc gtcgagtgtc gcgggcaggt
     4441 cctgtgccac cctgagagtg aggttgccgt tggtcgtgtg gacgtcgacg gccgttccat
     4501 cgagagctgc tacctcgcac tctatgctcc cgt
//
LOCUS       DATMZD010000017         2264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_587,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000017 DATMZD010000000
VERSION     DATMZD010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(191..577)
                     /locus_tag="VN478_00325"
     CDS             complement(191..577)
                     /locus_tag="VN478_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21160.1"
                     /translation="MSIIIHKTGNREYAYVAHRAGTRMVQSYIGPLARPDVRRRIDAA
                     RWATTMPEHTMRLFGGEDLDTVHLQKHAAAIIARLLEQGDLEDLRWMTCVYPTSAVID
                     VLLSASGVSPMARNFWMVWFGVLDAS"
     gene            complement(773..1525)
                     /locus_tag="VN478_00330"
     CDS             complement(773..1525)
                     /locus_tag="VN478_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21161.1"
                     /translation="MRTPSVVVLMTATVNPRGMVRTVVSDPDIRLREYHAVIRDATSQ
                     LPGLPIVVCDNSGRGVEQVAQGEAPVSWLDQVQFISFDDHEEAVRRGKGYGELGIIEY
                     ALANVPAVSAANLVVKVTGRYRVANIGSLVHEIAGLGPVDVVVDMVAGLTQADCRFFA
                     ASPGFLIGYLTPLRESIDDEHDVCFEHVLARAVHRAMADGLVWRPMPDVPVIVGRSGT
                     NGRPYASGRGRVMIRRVRKWMHRWSEARPLIH"
     gene            complement(1522..>2264)
                     /locus_tag="VN478_00335"
     CDS             complement(1522..>2264)
                     /locus_tag="VN478_00335"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HWQ21162.1"
                     /translation="LYPSPSLGDERGGQIQDVLQRRGQSYVVGNVVVESDPGAIADCF
                     VREQYRLEGRVEVRRGDTVLDLGAYKGESSIWMASQVGPSGLVLAFEPNPGARAFLER
                     NIARAAGSGIGKIEILAVAAGSSRHPGGFVSTAEGCSRLDADGDISVDVTTVDDIVSE
                     HALAGVDFIKMDIEGGEVEALRGARQTILQYAPRLAISVYHLARDLPEVVAAIRGVRS
                     DYRFFLSHKSPGLAETMLFASIDDGCRP"
BASE COUNT          476 a          764 c          622 g          402 t
ORIGIN      
        1 cacaagcgcc gacagttcgt caaagacctc cgacgccctg gacgcacgct ctgcaaagaa
       61 ctcgagcccg ccagagacac ggtgacccag gtgaagcgtc agtcccgtcc caccagccag
      121 caccccgcca tgcatcccca gggtcggtcc cagacgactc atgaggacac gagtgtcctc
      181 ctccacgcac tcaggatgca tcgagcaccc caaaccacac catccagaag ttgcgcgcca
      241 tgggcgacac accgctggca gaaagcagga cgtcaatgac ggcacttgtc gggtacacgc
      301 aagtcatcca gcgcaggtcc tccaggtccc cttgctcgag cagacgcgca atgattgccg
      361 cggcgtgttt ctgcagatgg acggtgtcca ggtcttcccc gccaaacagt cgcatcgtgt
      421 gctccggcat cgtcgtcgcc catcgcgcag cgtcgattcg gcggcgtacg tcgggtctcg
      481 cgagcgggcc gatatacgac tgaaccatcc gagtcccagc gcgatgagcc acataggcat
      541 attccctgtt tcctgtcttg tggatgatga tgctcatgca gagcctcctg cgagcatcat
      601 acacatcttg atatacaagt caaccacgtg tttacatatc atgctataca attcggtcaa
      661 ttcactccca cggacaagtg ttcaggaaca tcaggggtga ccgcgatagc ggaattgcca
      721 cggcgcctga ccaatgagcg actctgttgc acagagtcga caactgaccc gatcaatgga
      781 tcaggggccg tgcctccgac catcggtgca tccacttgcg cacccggcga atcatcaccc
      841 gacccctccc tgaagcatac gggcgaccgt ttgtaccgct ccgcccgacg ataacgggga
      901 cgtccggcat ggggcgccag acgaggccgt cggccattgc ccgatgcaca gcccgcgcca
      961 gcacgtgctc aaagcagaca tcgtgctcat cgtcaatgct ctcgcgcagt ggagtgaggt
     1021 agcctatgag gaagccggga gacgctgcga agaaacgaca gtcagcctgt gtcagaccgg
     1081 cgaccatatc tacgaccacg tcgacagggc ccaagccggc gatctcgtgg accagcgacc
     1141 cgatgtttgc cacccggtac cgcccagtca ccttcacgac gaggttggca gcactgacgg
     1201 ccggtacatt ggcaagggcg tactcgatga tccccaactc tccatagccc ttgcctcgac
     1261 gtactgcctc ttcgtggtcg tcaaacgaga tgaactgcac ctggtcaagc catgacaccg
     1321 gagcctctcc ttgggcgacc tgttcaactc ctcgtcccga gttgtcacag accacaattg
     1381 gaagcccagg aagctgcgac gtcgcgtcac gaatgaccgc gtggtattcg cgaagtcgga
     1441 tgtcggggtc gctcacgacg gtcctgacca tgccacgtgg gttcaccgtg gcagtcatca
     1501 ggacgaccac ggaaggcgtc ctcacggtcg acacccgtcg tcgatgctcg caaacaacat
     1561 cgtttcggcc agaccgggcg atttgtgaga caggaagaat cgatagtcgg accgaactcc
     1621 cctgatcgct gcgacgacct ccggcaggtc tcgcgcaagg tgatacaccg agatggcgag
     1681 gcgcggagcg tactggagga tcgtctgccg ggcgcccctg agcgcctcga cttccccacc
     1741 ttcgatgtcc atcttgatga agtccactcc cgccagcgcg tgctccgaaa cgatatcatc
     1801 cacggtcgtc acatcgacag agatgtcgcc gtcggcgtcg agcctcgaac acccttccgc
     1861 cgtgctgaca aaacccccag ggtgccgact cgaacccgcc gccaccgcga ggatctcgat
     1921 cttcccaatg cctgagcccg cagcgcgcgc gatattgcgt tccagaaagg ctctggcacc
     1981 agggttgggc tcgaaggcca gtacaagccc ggacggtccc acctggctcg ccatccagat
     2041 actgctttcg cccttgtatg cgcccaggtc cagcacagtg tctccccgac gcacctcgac
     2101 gcgtccctcg agtcgatact gctctcgaac gaagcagtcg gcgatggcac cagggtcgga
     2161 ttcgacgacg acgttgccga ccacataact ctgtcctcgc ctctgcagaa catcctggat
     2221 ctgtcccccg cgttcgtcgc cgagagatgg cgaaggatac agtc
//
LOCUS       DATMZD010000018         2406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_10596,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000018 DATMZD010000000
VERSION     DATMZD010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10596"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="VN478_00340"
     CDS             complement(<1..491)
                     /locus_tag="VN478_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015957660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWQ21163.1"
                     /translation="MAHDNTLLIRGARLADSGPEATCDVLIRSGIIAAVKNAPRLEQK
                     DSGERGLSSDGASIEAGGLTCLPAFCDMHAHFRDPGMTNKEDLASGSRAAVRGGYTAV
                     NLMANTKPVISTHAQVKDVLARAKRIGLVDVHQCVSITRDMDGRTVDHLEGLGKHVRI
                     ISE"
     gene            complement(493..924)
                     /locus_tag="VN478_00345"
     CDS             complement(493..924)
                     /locus_tag="VN478_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HWQ21164.1"
                     /translation="MLTITSIRNGIVIDHIRAGLGIRMFYQLGLDKADYTVALIMNAT
                     STRMGRKDIIKIENTADVDITMLALIDPNVTIDIIVDEHIVRKVKPELPERVEDVIKC
                     KNPRCITSVEKYIPHIFTLIDREKGQYRCQYCDEIYTVGKD"
     gene            complement(926..1846)
                     /gene="pyrB"
                     /locus_tag="VN478_00350"
     CDS             complement(926..1846)
                     /gene="pyrB"
                     /locus_tag="VN478_00350"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010293980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HWQ21165.1"
                     /translation="MLKGRHLLDPTDFSPGELDEIFDFADQIIQSPAQFNHLCDSSIL
                     ATLFYEPSTRTRFSFEAAMLRLGGGVVGFSEPTSSSVAKGESVADTIRTVACYADIIV
                     MRHPKEGAPRIASQYSTVPVINAGDGGHLHPTQTLTDLLTIRSVKHHLTGLTIGCCGD
                     LKFGRTVHSLIKATSRYPGNRFVLISPRELRVPDYVTADLAAKGIAFEEVERLDEVIG
                     DLDILYMTRVQKERFFNEDDYVRLKDVYILDAEKMKLARKDMIVMHPLPRVGEIAYDV
                     DDDPRAYYFKQAKFGMYVRMALIAKLLGRS"
     gene            2126..>2406
                     /locus_tag="VN478_00355"
     CDS             2126..>2406
                     /locus_tag="VN478_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004606626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HWQ21166.1"
                     /translation="MSIVMKGSEVAAAMKEGLLQDVERLDQKGIAPQLGVVRVGARPD
                     DLAYERGAVKRMEAVGIRCKVVELPESISQIEFEREFSVVNSDPDIHGIL"
BASE COUNT          433 a          651 c          799 g          523 t
ORIGIN      
        1 tcctcgctga tgatgcggac gtgcttgccc aggccctcca gatggtccac cgtcctgccg
       61 tccatgtcgc gggtgatgga gacgcactgg tggacgtcca caagtccgat gcgctttgcc
      121 cgcgcaagga cgtccttcac ctgggcgtga gtactgatga caggcttcgt gttggccatg
      181 agattcacgg cggtgtagcc gccgcgtaca gctgcccggc tgccgctggc gaggtcttcc
      241 ttgtttgtca ttccggggtc gcggaagtgg gcgtgcatgt cgcaaaaagc gggcaggcac
      301 gtgagcccgc ccgcttcgat ggaagcgccg tctgaggaga gtcccctctc ccccgagtcc
      361 ttttgttcaa gacgaggggc gttcttgacg gcggcaataa tgccgctccg gatgagaacg
      421 tcgcaggtcg cctccggacc actgtccgcg aggcgggcgc cacggatgag cagggtgttg
      481 tcgtgtgcca tgctagtctt tgcccaccgt gtagatctcg tcgcagtact ggcagcggta
      541 ctgtcccttc tcgcggtcga tcagcgtgaa gatgtgcggg atgtacttct cgacggaggt
      601 gatgcagcgg gggttcttgc acttgatgac gtcttcgaca cgctcgggca gctctggctt
      661 caccttgcgc acgatgtgct cgtccacgat gatgtcgatg gtcacattcg ggtcgatcag
      721 ggccagcatg gtgatgtcga catccgccgt gttctcgatc ttgatgatgt ccttgcggcc
      781 catgcgggtg ctggtggcgt tcatgatgag cgccacggtg tagtcggcct tgtccagccc
      841 cagctggtag aacatgcgga tacccaggcc ggcgcgaatg tggtcgatga cgatgccgtt
      901 cctgatgctc gtgatggtca acatctcaac tccttcccag cagcttcgcg atcagggcca
      961 tacggacgta catgccgaac ttcgcctgct tgaagtagta ggcacgcggg tcgtcgtcga
     1021 cgtcgtaggc gatctctccc acgcgcggca gcgggtgcat gacgatcatg tccttgcggg
     1081 cgagcttcat cttctctgcg tccaggatgt agacgtcctt gaggcgcacg taatcgtctt
     1141 cgttgaagaa gcgctccttc tgaacgcgcg tcatgtagag gatgtccagg tcgccgatca
     1201 cctcgtcgag gcgctcgact tcctcgaatg cgatgccttt ggcggcaagg tccgcggtga
     1261 catagtcggg gacgcgcagc tccctggggg agatgagcac gaaccggttg ccggggtagc
     1321 gtgaggtggc cttgatgagc gaatggaccg tgcggccgaa cttgaggtct ccgcagcagc
     1381 cgatggtgag tccagtgagg tggtgcttca cggagcggat cgtcagcagg tctgtcagcg
     1441 tctgcgtcgg gtgcaggtga ccaccgtcgc ctgcgttgat gacggggacg gtcgaatact
     1501 ggctggcaat gcgaggcgcc ccttccttgg ggtggcgcat gacgatgatg tcggcgtagc
     1561 acgccactgt gcggatcgtg tctgcgacac tctccccctt ggcgacagag ctcgaggtcg
     1621 gttcagagaa gccgacgacc cccccgccga gacggagcat ggcagcctcg aaactgaaac
     1681 gggtgcgggt ggagggttcg tagaagagtg ttgcgaggat ggaagagtca cacaagtggt
     1741 tgaattgagc gggagactgg atgatctggt ctgcgaagtc gaaaatctcg tcaagctccc
     1801 cgggactgaa gtccgtcggg tcaagcaggt gcctgccttt gagcatactg ttcccccatt
     1861 gtctatctac tggggtacgt gaccctttga gagtatacga gagtgaggtg ttcgtacaag
     1921 agagattctt cacgggtcga tgacacaatt gaggattcca cttcgggagg gttcgttcat
     1981 tatgctgctt gttcagatgc attccgtccc gtccttggca ttcatgggag tgcgtctatc
     2041 atgttcccct ggagtgtgac cggcccggcc gcgggagaca atgcgacgac tgacaggcat
     2101 acgatgaagt ggaggctgtg atccgatgag catcgtcatg aaaggctccg aagtggctgc
     2161 tgccatgaag gaaggactgc tgcaggatgt ggagaggctc gatcagaagg ggattgcccc
     2221 gcagcttggt gtcgtgagag ttggcgcgcg gcctgacgat ctggcctacg agcgaggcgc
     2281 agtcaagagg atggaggcgg tcggcattcg ctgcaaggtg gtcgagctgc cagagtcgat
     2341 cagtcagatt gaattcgaac gagagttctc cgtcgtcaac agcgatccag acatccacgg
     2401 catcct
//
LOCUS       DATMZD010000019         7480 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_30861,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000019 DATMZD010000000
VERSION     DATMZD010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7480
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30861"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(423..1547)
                     /locus_tag="VN478_00360"
     CDS             complement(423..1547)
                     /locus_tag="VN478_00360"
                     /inference="COORDINATES: protein motif:HMM:NF022447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HWQ21167.1"
                     /translation="MTSLIPYDYEASRERFCSSLRHLASRWPSSRVDRRLVDTADDGL
                     SIDILHAPATGQAKHMLLVTTGEHGIEGFVGSAIMELAVREILPTVDPAVTSLCLVHA
                     INPWGMKHEQRTNAANVDLNRNFVVSWDCLENANDGYRNLRHIFQPQGPMPTVPWNRL
                     LVASQVMRLALTGQSGKLRQALLAGQYEDPAGLYYGGTQWQPETQVMRNVYDDCLKAA
                     PHLVHVDLHTGYGPRDRMTIVNSGLDPVPSAAWARRIGYPSVVAATSQEFYRIEGDMI
                     DYMYRTRETGASSLAMYATCFEFGCLGDSLPAQIDSLWRAVASNRLRQHGAARDDVAE
                     KVRRLWRQAYCPSEPAWEEQAHRDALRAFRGILHDQGLTT"
     gene            complement(1552..2997)
                     /locus_tag="VN478_00365"
     CDS             complement(1552..2997)
                     /locus_tag="VN478_00365"
                     /inference="COORDINATES: protein motif:HMM:NF015563.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HWQ21168.1"
                     /translation="MEQQQSGARIGRRAFISAALILLVLMIGAGVLTRLVPAGRYQRS
                     VQDGREVLDPTSFSLTQARPLPVWRWFTAPVEVLGSSDAVMVITIILFILLVGGSFAV
                     LDHTGVIRTIIARIVTRFRSRRFVLIAVICLFFMGMGALLGIFEETVPLIPIVVALAW
                     SMGWDSLTGLGMSLLATGFGFSAAIANPFSIGVAQGIAGLPLFSGAWFRIIVFAVMYV
                     ILTTFVVVHARRVEKHSSFDQSWPEEQAARARYRQASDGVTFETPRRAVTWSVCCLGA
                     MLAVVLLGPLVPALQTYSLPLIGLAFVTAGLGAGFMAGLGARDTLRTFLSGLAGMAPG
                     ILLILMAMSVKHIVAEAGIMDTILHGAATGISKTSPSVASLLIYAVTLGMNFFIGSAS
                     AKAFLMMPLLAPLADLVGLTRQIAVTAFCFGDGFSNLVYPTNAVLLIGLGLTSVSYPR
                     WFRWTIVLQLITLVVTAAFLLLAVSLHFGPF"
     gene            complement(3093..3842)
                     /locus_tag="VN478_00370"
     CDS             complement(3093..3842)
                     /locus_tag="VN478_00370"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HWQ21169.1"
                     /translation="MNVSLVVHRVGADTAKNIGQMDLAVTQSAKAGAHLVLFSEAATT
                     GLILNNDPRHDLMLGEPVPGPAVTHFADLAKSSRIHIAFGILERDGAALYDTAVLVGP
                     SGEMLLKYRRIDPHWHDQMSDSKLYREGTECPAVDTPIGRFSFAICGDLFSDAVAEQV
                     RQQNPNYVLMPFARSFDSGLYDQKRWETEERQIYIDRVQKMGVTTLMANSMGAKDFDG
                     GSFGGAMVVHANGRITHSLHLGMPGMIVAGI"
     gene            complement(3993..4700)
                     /locus_tag="VN478_00375"
     CDS             complement(3993..4700)
                     /locus_tag="VN478_00375"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWQ21170.1"
                     /translation="MHRTPHTMFIVLLAVVLTMNCGCAARTPASPEKDIKGPLQPAKN
                     LGTVEISKYEGKNLSDFKTLEDNSVRGPQTVDITSYRLVIDGAVQTPQRLAYGDILSA
                     ARYQKLITLHCVEGWSATGLFEGVLMKDLLARAGPLPSAVTVIFHGHDDYTTSLPLAT
                     VLERNLLLAYRVNNADLIARNGYPFQLAAEDKLGYKWCKWVVRIELSTDPDYKGFWER
                     QGYGNDAEVNPSPQFDP"
     gene            5091..6311
                     /gene="metK"
                     /locus_tag="VN478_00380"
     CDS             5091..6311
                     /gene="metK"
                     /locus_tag="VN478_00380"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HWQ21171.1"
                     /translation="MERTHLFTSEAVTEGHPDKVADAVSDAVVDAYLEQDPFAHVAVE
                     TMMQGTLAIVAGEVAARAGTSVPVEQIVRDTARRIGYDRDEYGFNADTCHVMVAIDRQ
                     SSDIKQGVDDALESRESGDASLSDVLERTGAGDQGFMFGFACDETPVLMPFPAYMANK
                     MARRLAQVRKDGTLAYLRPDGKTQVTVEYEGDTPVRVTAVVVSTQHDPDVTHDRIVRD
                     MIEHVIRPVLPSGMLDAETRYYINPTGRFVIGGPAADTGLTGRKIIVDTYGGMGRHGG
                     GAFSGKDPTKVDRSAAMAARYCAKNIVASGLATRCEVQIAYAIGVAHPLSVYVDTFGT
                     STIDEEVIEKALVQVVDLRPEAIIRYFNLRRPIYGAIACYGAFGRDDLDLPWERTDIA
                     DKLRSLVRQQEAVV"
     gene            complement(6377..7222)
                     /locus_tag="VN478_00385"
     CDS             complement(6377..7222)
                     /locus_tag="VN478_00385"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HWQ21172.1"
                     /translation="MILGAHVSIAGGVANAPQNALDCTCDTFQIFTKSQLQWQAPPLS
                     RDEIAAFKRGMRSGHLVPGTVHAAYLVNLAAREPALLAKSRDCMVSDLGRTERLGIPY
                     LVVHPGAHRGQGAQKGIEVAARSLNFVFRHSRARRTMILLEATTGAGSVLGGTFEELA
                     VIRAGCRFPARVGFCIDTCHIFGAGYDIRSREGYAATIHDLDRALGLDNVRAFHLNDS
                     TGALGSKIDRHAEIGEGEIGLEPFRLLLSDTRFARTPGILETPGGDGAYARNLAVLRS
                     LIPSP"
     gene            complement(7219..>7480)
                     /locus_tag="VN478_00390"
     CDS             complement(7219..>7480)
                     /locus_tag="VN478_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21173.1"
                     /translation="LGGAQPSGFDFGAFTIAAGTDALETLAEICKRHPMREDQAIQLL
                     VNSGIDPLPVLDTMRVDTRFRIERYGGQTFFLVRETDGEPDT"
BASE COUNT         1432 a         2241 c         2391 g         1416 t
ORIGIN      
        1 cggacgtcac cagcagttgt gagacgtcca aagtcgctat gccgaggaaa gacgaacagg
       61 atcgtctggt ccatgctgcc tccaatcggg ggcgctgcac gaaacgctct ctgcaagcta
      121 caacggattc tactagcgcg cggatgatga cctctggctt ctctactgcc acgccgatgc
      181 tgagtacgtt acggacccga acctcaacac atccctgact gcctggacaa tggtgcgttc
      241 tgtctccacc gtacttgcca caggaacctg atacctatct ggcgtcagga taggtcgatg
      301 ggcattcttg tcaaggccgg tccgtcaggt gggcatgaac cagcatgaat acagacgaag
      361 acagccatca gacgacaggc cgccatgacc cgcgtcggcg gctgtcagtt ttcgccttcc
      421 gttcaggtgg tcaggccctg gtcgtggagg atgcctcgga atgcccgcag ggcatctctg
      481 tgggcctgtt cttcccacgc aggttctgac gggcagtatg cctggcgcca gagcctccgg
      541 accttctctg caacgtcgtc gcgggcggcg ccgtgctggc gcaggcgatt gctcgccacc
      601 gcacgccaca ggctgtcgat ctgggccgga agcgaatcac caagacatcc gaactcgaag
      661 cacgtcgcgt acattgcgag gctggaagcg ccggtctcgc gcgtccgata catgtagtcg
      721 atcatgtcgc cctcgatgcg gtagaactcc tgcgacgttg ctgcaaccac gctcgggtag
      781 ccgatacgtc gggcccacgc tgcagacggc accggatcca gtcccgagtt gacgatggtc
      841 atgcggtcgc ggggtccata cccggtgtgc agatcgacgt gcaccaggtg cggagccgct
      901 ttcagacagt cgtcgtatac gtttcgcatg acctgcgtct ccggctgcca ttgcgttccc
      961 ccgtagtaga gacctgccgg gtcttcatac tgcccggcaa gaagggcctg ccggagcttc
     1021 ccggactggc ccgtcagggc aagacgcatg acctggctcg cgacgagcag tcggttccac
     1081 ggaaccgtag gcatgggccc ctggggctgg aagatatgcc ggaggttccg atagccgtcg
     1141 ttggcattct ccagacagtc ccaggacacc acgaagttgc ggttcaggtc gacgtttgcc
     1201 gcgtttgttc gctgttcgtg cttcatcccc cacgggttga tggcgtgaac caggcacaat
     1261 gacgtcactg ccgggtccac cgtcgggagg atctcccgaa cggccagctc catgatcgca
     1321 gagccgacga atccctcgat gccatgttca cccgtcgtca cgagcagcat gtgctttgcc
     1381 tgtccggtcg caggagcgtg caggatgtcg atgctcagcc catcgtcggc tgtgtcgacc
     1441 agccggcgat cgaccctgct ggagggccag cgggacgcga gatggcggag gcttgagcag
     1501 aagcgctcac gtgacgcttc atagtcgtac gggatcagcg acgtcatggg gctagaaggg
     1561 accgaagtgc agcgagaccg caagcaacag gaatgcggcg gtcaccacga gggttatgag
     1621 ttgaaggacg atcgtccagc ggaaccatcg cggataggac acactggtca gcccaaggcc
     1681 gatcagaagg acggcgttcg tcgggtacac gaggttgctg aacccgtctc cgaagcagaa
     1741 cgccgtaact gcgatctgcc gcgtgagtcc gaccaggtcc gcaagcggtg caaggagcgg
     1801 catcatgagg aaggccttgg cggacgcgga tccgatgaag aagttcatcc cgagagtcac
     1861 ggcgtagatg agcaggctgg cgaccgaggg cgaggtcttc gagatgcccg tggctgctcc
     1921 gtggaggatc gtgtccatga ttccggcttc ggcgacgatg tgcttgacgc tcatcgccat
     1981 caggatgagc agtatgccgg gagccatgcc tgccaggccg gacaggaagg tcctgagcgt
     2041 gtcgcgtgct ccgaggccgg ccatgaaccc ggcgcccagg ccggctgtca cgaaggccag
     2101 gccgatgagt ggcagggagt acgtctggag cgcagggacc aggggcccca gcagaacgac
     2161 ggccagcatg gcgccgagac agcagacaga ccacgtcact gcccgccggg gcgtctcaaa
     2221 ggtgacgccg tcggatgcct gccggtaccg cgcccgggct gcctgttcct ccggccatga
     2281 ctgatcgaac gaggagtgct tctcgacgcg gcgagcgtgc acgacgacga acgtcgtgag
     2341 gatgacatac atgactgcaa agacgatgat gcggaaccat gcccctgaga acaagggcag
     2401 tccggcgata ccctgggcga cgccgatgct gaacgggttg gcgatagccg cgctgaaccc
     2461 gaatccggtg gcgagcagac tcataccgag tcccgtgagg gaatcccagc ccatggacca
     2521 tgccagggcg acgacgatgg gaatgagagg cactgtctcc tcgaagatgc ccagcagtgc
     2581 ccccatgccc atgaagaaca ggcagatgac tgcgatcagc acaaatctgc gcgaccggaa
     2641 cctggtcacg atgcgagcga tgatggtgcg gatgacgccc gtgtggtcca ggacggcgaa
     2701 gctgcctccc acgagcagga tgaacaggat gatcgtgatg accatgacgg cgtcgctgct
     2761 gccaaggact tccacggggg cggtgaacca cctccatacg gggagtgggc gcgcctgcgt
     2821 caggctgaac gacgtggggt cgagcacttc gcgtccgtcc tgcacgctgc gctgataccg
     2881 gccggcagga accaggcgag tcaggacccc cgcaccgatc atgaggacaa gaaggatcag
     2941 tgccgccgag atgaacgcgc gccggccgat tcgtgctcct gactgttgtt gttccacgat
     3001 tcctccccgt tctacagtga gacaggccct ctttgcgatg agggcctgtc ctggttcact
     3061 accgtttgca gcaagagctc agcgtcattc tcctagatgc cagcgacgat catcccgggc
     3121 attcccaggt gaagcgagtg cgtgatgcgc ccattggcgt ggacgaccat ggcgcctcca
     3181 aacgagcctc catcgaagtc cttcgccccc atcgagtttg ccatgagcgt tgtgacgccc
     3241 atcttctgca cccggtcgat atagatctgg cgttcctcgg tctcccatcg cttctggtca
     3301 tacaggccgc tgtcgaaact gcgcgcgaag ggcatgagaa cgtagtttgg attctgctgg
     3361 cgcacctgtt cggcgactgc atcgctgaac aggtcgccac agatggcaaa ggagaagcga
     3421 ccgatgggag tgtcgaccgc ggggcactct gttccctcgc gatacagctt cgagtcgctc
     3481 atctggtcgt gccagtgcgg atcgatgcgg cggtacttga ggagcatctc tccgctcgga
     3541 cccacaagca cagccgtgtc gtacagtgcc gccccgtcac gttcaaggat gccgaacgcg
     3601 atgtggatgc ggcttgactt ggcgagatcg gcgaaatgag tcaccgcagg tccagggacc
     3661 ggttctccca gcatgaggtc atgcctggga tcgttgttga ggatcagccc cgtggtcgca
     3721 gcctctgaga acaggacgag gtgagccccc gcttttgccg actgtgtcac tgcaaggtcc
     3781 atttgcccga tgttctttgc tgtgtccgcc ccaacgcgat gcaccacaag tgatacattc
     3841 atcctcgctc ctccttttca ggttcgacgc ctgccagccg ccgctcgatg ccaaagccct
     3901 ccaggccgag ggagtgtcaa gtgtcctggt gtcaggatag ttgaacatcc ctcttttgca
     3961 acatgccctt cggacggcgg gtatttgatg cctcacggat cgaactgggg gctcgggttg
     4021 acctcggcat cgttcccgta tccctgacgt tcccagaatc ccttgtagtc cgggtcggtg
     4081 gacagctcga tacgcacgac ccacttgcac cacttgtacc cgagcttgtc ctcggctgcc
     4141 agctggaacg gatacccgtt ccgggcaatc aggtccgcgt tgttgacacg gtacgcaagc
     4201 agcaggttgc gttcgagcac tgtggcgaga ggcagggacg tagtatagtc atcatggccg
     4261 tggaagatga cggtgacggc cgagggaagg ggtccggccc tggccagaag gtccttcata
     4321 aggacaccct cgaagagtcc tgttgcactc catccctcta cgcaatgcag cgtgatgagc
     4381 ttctggtagc gggcagcaga gaggatgtca ccatacgcaa gacgctgggg agtctgcacg
     4441 gccccgtcga tgacgagccg atatgacgtg atgtccacgg tctgcgggcc ccggacggag
     4501 ttgtcctcaa gtgtcttgaa atcactcagg ttcttgcctt cgtacttgct gatctcgacg
     4561 gttccgaggt tctttgccgg ctgcagcgga cccttgatgt ccttctccgg ggacgcgggc
     4621 gtccgggcag cgcatccaca gttcatggtg agaacgacag ccagcaggac gatgaacatc
     4681 gtgtgcggag tcctgtgcac ggttactcct ttccgggtca tggactgatg ggcccgacgt
     4741 gaaaaggtgt cctgcctgca gtgtagcatc gcccccagca atcacaacgc tcgcaggacg
     4801 cgacgtccgg catcatgttc catcacagtt gcaggccttg gcgccaatga tgagctcgat
     4861 ataatcatgt gcgacgcggt cagttaacgg ccacttgctc cgcgataaaa atgtgctaaa
     4921 gcgccgagga acgacggacc agtgtcgcct cacgctgtga cgtggggcga ttttcatttc
     4981 agtcacctct ctcagcagtg cgacagcgac gccccgtggg tgcgacgaca gtcacgcgaa
     5041 cggccgtgga tcccatgcgg gtcctttgtc atgaagcggg aggaacgact atggaacgga
     5101 cacatctctt tacatctgaa gcggtgaccg agggacatcc ggacaaggtt gcagacgctg
     5161 tctcggacgc tgtcgtcgac gcatatctcg aacaggatcc gtttgcccac gtcgcagtcg
     5221 agaccatgat gcagggaacg ctggcgatcg tcgccggcga ggtagctgcc cgtgcaggca
     5281 cctcggtgcc cgtcgaacag atcgtgcgcg atacggcaag gcgcatcggc tatgaccgcg
     5341 acgagtacgg gttcaatgca gacacctgtc atgtcatggt ggcaatcgac cggcagtcca
     5401 gcgacatcaa gcagggagtc gacgacgctc tcgagtcccg tgagtcgggg gatgcgtcct
     5461 tgtccgacgt gctggagcgg accggcgcgg gcgatcaggg cttcatgttc ggattcgcct
     5521 gtgacgagac gcctgtcctc atgccgttcc cggcctacat ggccaacaag atggctcgtc
     5581 gtctcgccca ggtccgaaag gatggcactc tggcgtatct ccgacctgat ggcaagacgc
     5641 aggtgaccgt cgaatacgag ggtgacactc cagtgcgcgt caccgcagtc gtcgtctcca
     5701 cacaacatga ccctgacgtg acacacgacc ggatcgtccg tgacatgatc gagcacgtca
     5761 tccggccggt cctgccttcc ggcatgctgg atgcggagac gaggtactac atcaacccga
     5821 ctggcaggtt cgtcattgga ggtccggcag ccgacacggg gctgaccggt cgcaagatca
     5881 ttgttgatac ctatggtggc atgggtcgtc acggtggagg cgcgttctcc ggcaaggacc
     5941 cgaccaaggt cgaccgctcg gcagccatgg cggcgaggta ctgtgccaag aacatcgtcg
     6001 cttcagggct ggccacgagg tgcgaggtcc agatcgccta tgcgatcggt gtcgcccacc
     6061 cgctgtccgt ctatgtggat acgtttggga cctccacgat cgatgaagag gtgatcgaga
     6121 aggcgctggt acaggtggtc gacctgagac ccgaggccat catccggtac ttcaacctgc
     6181 ggcgaccgat ctacggtgcc atcgcatgtt atggcgcctt tggccgcgac gacctcgacc
     6241 tgccctggga gcggactgac attgcggaca agctgcgttc gctggtcagg cagcaggagg
     6301 ctgttgtcta gctcgtccat gtctgcagga cgctgattgt ggatcccctt gtgcctggta
     6361 caaggggatc cacaattcat ggagatggga tcagggagcg gaggaccgcg aggttgcggg
     6421 catatgcgcc gtcccctccg ggcgtctcca gaatgcctgg cgtccgtgcg aaccgggtgt
     6481 ccgagagcag gagacggaag ggttcgaggc cgatctcgcc ctccccgatc tccgcgtgcc
     6541 tgtcgatctt cgaaccaagg gcgcccgtgg agtcattgag atgaaaggct ctgacgttgt
     6601 cgagtccgag ggcacggtcc aggtcgtgga tggttgcggc gtagccttcc cgcgagcgga
     6661 tgtcgtagcc tgcgccgaag atgtgacacg tgtcgatgca gaaacccacg cgcgcgggga
     6721 agcggcatcc ggcgcgtatg acagccaatt cctcgaaggt gcctcccagg accgagcctg
     6781 cgcccgtggt ggcttcgagc aggatcatcg tgcggcgggc tcgggaatgg cggaagacga
     6841 agttcaggct cctggcagcc acctcgatcc ctttctgggc cccctgtcca cggtgggcgc
     6901 ccgggtgtac gacgaggtag gggattccca ggcgctccgt cctgccaagg tcgctgacca
     6961 tgcagtctct ggactttgcc agcagggcgg gctctcgggc ggcgaggttg acgaggtacg
     7021 cagcatggac cgttccagga acaaggtgac ccgaccgcat gccgcgtttg aatgctgcga
     7081 tctcatcgcg ggatagtgga ggagcctgcc actgaagctg actcttcgtg aagatctgga
     7141 acgtgtcgca cgtgcagtcg agagcattct ggggagcatt tgcgactcct cccgcgatgg
     7201 acacgtgtgc gccgaggatc atgtgtccgg ctctccgtcc gtctcgcgga cgaggaagaa
     7261 cgtctgacca ccatatcgct cgatcctgaa gcgtgtgtcg actcgcatgg tgtccagcac
     7321 cggcaaaggg tcgatccctg agttgaccag cagctggatt gcctggtcct cgcgcatggg
     7381 gtgcctcttg cagatctcgg cgagggtctc cagggcgtcg gtgccggccg cgatcgtgaa
     7441 tgcgccgaag tcgaatccgg atggctgtgc tcctccaagc
//
LOCUS       DATMZD010000020         2488 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_141397,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000020 DATMZD010000000
VERSION     DATMZD010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141397"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VN478_00395"
     CDS             <1..265
                     /locus_tag="VN478_00395"
                     /inference="COORDINATES: protein motif:HMM:NF024013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3768 domain-containing protein"
                     /protein_id="HWQ21174.1"
                     /translation="FFMTQGVRQHPKGVEAMAYLLTCPAQMEESINPWGDCDMLKVIF
                     CGDTFYLKIDCYASDGTLNHASEDPADDQKTTRVFTCLLASEY"
     gene            complement(315..587)
                     /locus_tag="VN478_00400"
     CDS             complement(315..587)
                     /locus_tag="VN478_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21175.1"
                     /translation="MNPFRVFPAAMLLVAMTACAPSTEELTKDVRASMESKFTPKGIT
                     VKSLVLTKKGGNEYTGVLETQEPNGAFTYTVEVISDGKTMTWQVKQ"
     gene            complement(706..1545)
                     /locus_tag="VN478_00405"
     CDS             complement(706..1545)
                     /locus_tag="VN478_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21176.1"
                     /translation="MGSHQKYRMGRGEKIFLWGVGTLVVLGIISSLTIPYLLRQRERA
                     RDKTADTVVATSGMPATTPTAQTGTPSETPQGEPAQFDEKAWLERTRAKLNECKRLLT
                     SPDLYKDSYDLAANNIASAKVLTSALCTGTIPAKTPQVEALSSSLARGFVARLEAEIW
                     LREHDPSFKTYANRLDIGLKPEFDEYHLLYAIDDLDYGKAVEKLNQTQIRPSEINTSQ
                     DAYKKWLDQSQRVSANQNLVMRVAPEKLLVYFRLSHDNKPADLRTEISDQELIDHNED
                     STD"
     gene            complement(1554..2132)
                     /locus_tag="VN478_00410"
     CDS             complement(1554..2132)
                     /locus_tag="VN478_00410"
                     /inference="COORDINATES: protein motif:HMM:NF025597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4339 domain-containing protein"
                     /protein_id="HWQ21177.1"
                     /translation="MPAQDVWYFATPQGERTGPVTYQKLLDRIQSGQMNRDDLVWADH
                     LTEWTPMGQVPELAGWARQLPPTMPARPAVPNDQDIIESIKTFELVSAIVWCVIAGFQ
                     ILAGLCTGFFGCALVAAGAWNVYAAVTRFQLMKKIQERRKAVVMAYESYTQLVVIGLI
                     NLFLGAFIGALWVIFDFVIRDKVLKNRRLFDQ"
     gene            complement(2212..>2488)
                     /locus_tag="VN478_00415"
     CDS             complement(2212..>2488)
                     /locus_tag="VN478_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21178.1"
                     /translation="NQGDDASMRLLLPVGRSVWAVAAGYLALGALLPIFGILFGLAAV
                     ITGIIAQRAIHRDSHLHGMGRVIFAYIVGGLGILGWSAMLVYGLARR"
BASE COUNT          517 a          735 c          709 g          527 t
ORIGIN      
        1 gttcttcatg actcagggag tgaggcagca tccgaagggg gtcgaagcca tggcctacct
       61 tctcacctgc cccgcccaga tggaggagag catcaacccc tggggcgact gcgacatgct
      121 gaaggtgatc ttctgcggag acaccttcta cctgaagatc gactgctatg cctcggacgg
      181 gaccctgaat cacgccagcg aagatcccgc cgatgatcag aagaccaccc gtgtctttac
      241 ctgcctgttg gccagcgagt actaagagat tggcacgaga aacgccactc atctcttcat
      301 cacgccgatg attcctactg tttgacctgc catgtcatgg ttttgccgtc gctgatgact
      361 tcgacggtat acgtgaaagc cccgttcggt tcctgcgttt ccaggacccc cgtgtactcg
      421 ttgccgccct ttttcgtgag aaccaggctc ttgactgtaa tgcccttggg ggtgaattta
      481 ctctccatgc tggctcgcac gtctttggtc aactcctcgg tagatggggc acaggccgtc
      541 atcgccacga ggagcatggc ggcggggaac actcggaagg ggttcatgac ttctcctagg
      601 ttgaagacgc ggtggggcga ctacatgaaa agcacgggga tgagagcggt ggtgacaggt
      661 gcaaaccacc tcagaaacga catgccgaag catcatcgac ggtgatcaat ccgtgctgtc
      721 ctcgttgtga tcaatcagct cctggtcgct gatctcagtt cggaggtcag cgggcttgtt
      781 gtcatgggac aaccggaagt agacaagcag cttttccgga gcgacacgca tgaccaggtt
      841 ttgattggct gaaacgcgct gactctggtc cagccacttc ttgtaggcat cctgggacgt
      901 gttgatctcg gagggccgga tctgcgtttg attcagcttc tccactgctt tcccgtaatc
      961 caggtcatcg atggcataga gcagatggta ttcgtcgaac tcaggcttga gcccaatgtc
     1021 cagtcgattc gcatacgtct tgaagctcgg atcgtgttcc cgaagccaaa tctcggcttc
     1081 cagcctcgcg acaaaccccc gcgccagcga ggaggaaagt gcttcaacct gtggggtctt
     1141 ggcggggatg gtgcccgtac aaagcgcact ggtaagaact ttggcggaag caatgttgtt
     1201 ggcggctagg tcatagctgt ccttgtagag gtccgggctc gtcaggagac gcttgcactc
     1261 attgagcttc gcccgggtcc gctcgagcca tgctttctcg tcgaattgcg caggctcccc
     1321 ctgtggcgtc tcgctgggag tacccgtttg ggccgtcggg gtcgtggcgg gcattccgga
     1381 tgtcgcgacc acggtatcag ccgttttgtc cctagctcgt tcccgctgac gaagcagata
     1441 cggaatcgta aggctggaga tgattccgag cacgacaagc gtgccgaccc cccataagaa
     1501 gatcttctct ccacggccca tcctgtattt ctgatgactc cccacggcta ctcctattga
     1561 tcaaaaagac gccggttctt cagcaccttg tctcggatga cgaaatcgaa gatcacccac
     1621 aaggctccga tgaacgcccc aaggaacaga ttgatgaggc cgatcaccac cagctgggtg
     1681 tagctctcat aggccatcac gacagccttt cggcgctcct ggatcttctt catcaactgg
     1741 aaacgagtaa cggctgcgta cacgttccag gctccggctg cgaccagcgc acagccaaag
     1801 aacccagtgc agagacccgc aagaatctgg aacccggcga tgacgcacca gacgatcgcg
     1861 gaaaccagct cgaaggtctt gatggactcg atgatgtcct ggtcgttcgg aacagcgggc
     1921 cgcgcaggca tcgtcggtgg cagttgccga gcccaccctg caagctcggg aacctgtccc
     1981 atgggcgtcc attcggtgag gtggtctgcc cagaccagat catccctgtt catctgaccg
     2041 ctctggatcc gatccaggag cttctggtag gtgaccggcc cggttctctc cccttgcggt
     2101 gtcgcgaagt accacacatc ctgcgcaggc atgtctgccc tccatcgggt gacgtcagtg
     2161 aacgagccgt gtttccccag ggattcggat caatccgtcg gtattcatcc cctaacgccg
     2221 ggcaaggcca tagaccaaca tggcgctcca ccccaggatg ccgagccctc cgacgatgta
     2281 tgcgaagatg acgcggccca tgccatgcaa atgcgaatcg cggtggatgg cacgttgcgc
     2341 gatgatcccc gtgatcacgg ctgccagacc gaataggatg ccaaagattg gaagcaaagc
     2401 gccgagagcc aagtagccag cggcaacagc ccagacgcta cgcccgacag gaagcagaag
     2461 cctcatgctg gcgtcatcgc cttggttt
//
LOCUS       DATMZD010000021         6601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_61144,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000021 DATMZD010000000
VERSION     DATMZD010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6601
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61144"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="VN478_00420"
     CDS             <1..545
                     /locus_tag="VN478_00420"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HWQ21179.1"
                     /translation="PGHGYVSSTGYFDVGAVGPSGTKESSINLSIGLKVKILLEADGA
                     RVVMTRSDDSSRENPDLTKRTQIANSSGADLFLAIHQNATDAGASIGGTEVHYWFDQS
                     KTFATLIQKHLVSALGRADRGVQKTSLYLVSHIDTMPAALVECAFISNSEEERLLRED
                     SFQQKIAQAIADAIVEYFHK"
     gene            555..1343
                     /gene="murI"
                     /locus_tag="VN478_00425"
     CDS             555..1343
                     /gene="murI"
                     /locus_tag="VN478_00425"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HWQ21180.1"
                     /translation="MGSYIGVLDSGVGGLTVVRELKNRLPREAVMYVADSRYFPWGTK
                     PREQVIERATALVEFLKAHGARMVVVACNTASSVIFPDIQNRVDVPVLGMISAGVKSA
                     QLATRNGKVAVLSTPGTARSHGYLRAMGLMDPTVEMIELQSQELVNLVEAGTIEGQDV
                     SDFASDVLEPVREFGADTLVLGCTHFPFLNNLMRDILGPGVKIVDPSRELAYEVGLVL
                     EKCRLTTGGGQQDLFFTTGDPKDFLDKARLFLGDYVGTVEVADV"
     gene            1340..1948
                     /locus_tag="VN478_00430"
     CDS             1340..1948
                     /locus_tag="VN478_00430"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HWQ21181.1"
                     /translation="MRLLLVSGNVHKLQEFDAILDDVGCWLPLDELDVKGTVECGATY
                     YQNAYRKARAGLLSISANEAVDCAVIADDSGLELPVFGRMPGVRSARLQYGGLSERAA
                     LAAFLTQRGVSSTPARFVCWIVAFVPWSPRGLAWSGTVTGTVTPKALGSHGFGYDALF
                     TPDGYRLTFGEMDADVKNHISHRAQAVRVLWSSLQGALSLRP"
     gene            1982..2058
                     /locus_tag="VN478_00435"
     tRNA            1982..2058
                     /locus_tag="VN478_00435"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2016..2018,aa:Pro,seq:tgg)
     gene            2060..2136
                     /locus_tag="VN478_00440"
     tRNA            2060..2136
                     /locus_tag="VN478_00440"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2094..2096,aa:Arg,seq:tct)
     gene            2147..2223
                     /locus_tag="VN478_00445"
     tRNA            2147..2223
                     /locus_tag="VN478_00445"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2181..2183,aa:His,seq:gtg)
     gene            2265..2341
                     /locus_tag="VN478_00450"
     tRNA            2265..2341
                     /locus_tag="VN478_00450"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2299..2301,aa:Arg,seq:cct)
     gene            2356..2432
                     /locus_tag="VN478_00455"
     tRNA            2356..2432
                     /locus_tag="VN478_00455"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2390..2392,aa:Arg,seq:tcg)
     gene            2542..2617
                     /locus_tag="VN478_00460"
     tRNA            2542..2617
                     /locus_tag="VN478_00460"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2575..2577,aa:Lys,seq:ctt)
     gene            2664..2748
                     /locus_tag="VN478_00465"
     tRNA            2664..2748
                     /locus_tag="VN478_00465"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2698..2700,aa:Leu,seq:tag)
     gene            2763..4031
                     /locus_tag="VN478_00470"
     CDS             2763..4031
                     /locus_tag="VN478_00470"
                     /inference="COORDINATES: protein motif:HMM:NF017508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HWQ21182.1"
                     /translation="MEYTTTNRTGNSIVLSATFTADEVSAMRHEATLAMAGKLRIPGF
                     RPGKAPLSIVAKYVSDEELKDDVLQKAASQTYVTFLKEHKDTDALIFEPEIVEAAPED
                     SPTDGLKVDIRVFEMPKADREFWSTVEVEDVPVDTARAVQSRINALVDAVTETSPKDG
                     PAAQGDSVTVTISTERSKAPHHTEFTLGEGEVGKAYEPFVVGMNVGESKHFSVQMQES
                     ALEGEVTLEGVAMKHVPEVNDDFARTVGAFESLEQLRKTLEEDEQHKADEARKDAVFG
                     RAIEKAAEKLGIDFPGYVLRDATEERLGEIKNSLARNGLSLNEYLKYNDITVEQLTKD
                     VEADAVKLLRRDLLMEATERACSIVAGDADIDAYSESHKEELAKANVDTTTEDGRKTV
                     RNVIVWDNTRSLIVSAVQLAMTPEGKREAQ"
     gene            4028..4657
                     /gene="clpP"
                     /locus_tag="VN478_00475"
     CDS             4028..4657
                     /gene="clpP"
                     /locus_tag="VN478_00475"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HWQ21183.1"
                     /translation="MSVIPYVVEQTAHGERGYDIYSRLLKDRIVFLGGEIDTQVSNLI
                     VAEMLYLTAENATKDIYLYINSVGGEVTPGMAIYDTMQYINAPVSTICLGQAASLAAV
                     LLAAGRKGKRFALPNSRIMIHQPWGGAQGQATDIEIQAKEILRIKESVSSILASHTGK
                     PLDVVRRDTERDFFMSAEEAVDYGIVDQVISKIGVSGEVGLSGKNVQSQ"
     gene            4641..5879
                     /gene="clpX"
                     /locus_tag="VN478_00480"
     CDS             4641..5879
                     /gene="clpX"
                     /locus_tag="VN478_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006905399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HWQ21184.1"
                     /translation="MSSHNSEEPSCSFCGKKSSEVALLVKGLDGSFICDDCIERGSQL
                     MQESKHETRALDFKALRPKEIYARLNEYVIGQGEVKKALAVGAYNHYKRLRARKDSKV
                     EIEKSNILLAGPTGVGKTYLARTLARILDVPFTIADATTLTEAGYVGEDVENILLGLL
                     QATNFDVARAECGIVYIDEIDKIGRKSENPSITRDVSGEGVQQALLKIVEGTTANVPP
                     QGGRKHPLQEYIKFNTQDVLFIAGGTFDGLDTIRTLRATPREVGFVHEPADREGRRLL
                     SSDFVKFGMLPELVGRFPLKLELEELSVSDLKRILVEPKNSIVQQYQELFAMDGVALT
                     FSGSALERIAEKARDMGMGARGLKSVVDQPMVDLMFELPSFKEITEVTVGDDFFSNRH
                     EIIATRSDGSTERIPLQESA"
     gene            5898..>6601
                     /locus_tag="VN478_00485"
     CDS             5898..>6601
                     /locus_tag="VN478_00485"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HWQ21185.1"
                     /translation="MSERDWELSEAPLIPLRNVVVFPYHVLPLFVGRPKSLKSIESAL
                     AGNRKIVLVCQRDESNEDPGLADLYDIGVGAEILQLLKLPDGSDRVLVEGVRRVRITN
                     VQSDSDEMLVATVEPLPADEKTSTERQALMRVVLDQFSQYVELSRKLPSETVNNVSGV
                     TEPDRFADLVASSLLLGTEDRQALLGTVDAEERLHHIAELLAKEIYVLELARKIESDV
                     KAKVDQSQREYYLREQV"
BASE COUNT         1443 a         1794 c         2003 g         1361 t
ORIGIN      
        1 accccgggca cggctacgtg tcgtcgacgg gctactttga tgtgggggct gtcggcccgt
       61 cgggtacgaa ggaatcgtcg atcaacttgt cgatcggact caaggtgaag atcttgctgg
      121 aagctgacgg tgcccgcgtg gtcatgacgc ggtcggacga ttcgtctaga gagaaccctg
      181 acctcaccaa gaggactcag atcgcgaaca gttcgggggc cgacctcttc ctcgcaatcc
      241 accagaatgc cacggatgcg ggagcgtcga ttggaggcac agaagtccac tactggtttg
      301 accagtcaaa gacgttcgct acgctcatcc agaagcatct cgtttcggca ctggggaggg
      361 cggatcgagg cgtgcagaag acgtctctgt atctggtctc acacatcgac acgatgcctg
      421 cagcattggt cgagtgcgca ttcatcagca acagtgaaga ggagcgcctt ttgcgtgagg
      481 attccttcca gcagaagatt gcccaggcaa tcgccgacgc tatcgtcgag tacttccaca
      541 agtaggtggg gctggtggga tcatacattg gagtgctcga ttcgggcgtt ggtggcctga
      601 ctgtcgtccg ggagttgaag aaccggttgc ctcgtgaagc ggttatgtat gttgccgact
      661 cgaggtattt tccatggggc acgaagcctc gagaacaggt catcgagaga gctacggctc
      721 tcgtcgagtt cctcaaggcc cacggggcgc ggatggtcgt cgttgcctgc aatacggctt
      781 cgagcgtcat cttcccggac atccagaatc gggtcgacgt ccccgtcctg ggcatgatat
      841 cggctggagt gaagagtgcg cagcttgcga ctcgcaacgg caaggtcgca gtcctctcaa
      901 caccagggac agcgcgcagc cacggctatc tcagggccat ggggctcatg gatccaaccg
      961 tcgagatgat agagctccag tcacaggaac tggtcaacct ggtggaggcc ggaaccatcg
     1021 aaggtcagga cgtatccgac ttcgcttcag acgtgctgga gcccgtacgg gagttcggcg
     1081 cagacacgct ggtgctgggt tgcactcact tcccgtttct caacaacctc atgagggaca
     1141 tcctcggacc aggcgtcaag atcgtcgacc cgagcaggga actggcctat gaggtgggtc
     1201 tagtacttga gaagtgccga ttgactactg gcggaggaca gcaggacctc ttcttcacga
     1261 cgggcgaccc caaggatttc ctggacaaag cgcgcctgtt cctcggtgac tatgtcggga
     1321 cggtcgaggt cgcagacgtt tgagattgct acttgtgtcc ggcaacgtcc acaaactgca
     1381 ggagttcgat gccattctgg acgatgtcgg gtgttggctt cccctggacg agctggacgt
     1441 gaagggcacg gtcgagtgtg gggcgaccta ctatcagaat gcttaccgaa aggcccgggc
     1501 aggtctcctg agcatttcgg cgaacgaagc tgtcgactgc gccgtcatcg cagatgactc
     1561 cggccttgag ttgcctgtct tcggcagaat gcctggagtc cgttcggcgc ggctgcaata
     1621 cggaggcctc tcagagaggg ctgcgctcgc agcattcctc acgcagcgcg gtgtgtcctc
     1681 gacccctgcg cggttcgtgt gctggatcgt cgcattcgtg ccctggtcgc ctcggggtct
     1741 cgcctggtcg ggtactgtca ctggcaccgt gacgccgaaa gcgctggggt cccacggctt
     1801 cggatatgac gccctgttca cccctgacgg ctaccgcctc acattcggcg agatggatgc
     1861 agatgtcaag aaccacatca gccaccgcgc acaagctgtt cgggttctgt ggtcttctct
     1921 tcagggggca ttgtcgctga gaccttgatt ttcagctgat tgcgtgtatt ctctttctac
     1981 tcggggtgtg gcgtagcttg gtaacgcgca tggtttggga ccatgagatc ggtggttcaa
     2041 atccactcgc cccgaccatg ggcccatagc tcagctggat agagcaacgg atttctaatc
     2101 cgtcggtcgc aggtccaaat cctgctgggc tcaccagtct cacatggtgg gtatagctca
     2161 attggttaga gtactggact gtggatccag atgttgggag ttcgagtctc cttacccacc
     2221 ccatttttgc ggacgatcct ttatactgta cgctttgcac tactgcgctt atagctcagg
     2281 cggatagagc gatggattcc taatccgtag gccgcaggtt cgagtcctgc taagcgcacc
     2341 agtatcgtaa cgtgagcgcc catagctcaa ctggatagag ctgcggactt cggatccgcg
     2401 ggttgcgggt tcgactcctg ctgggcgcgc caacggtact ccgagccgcg agagtccaca
     2461 gagtggcata tcctgcgggt atggcgactg gatcgcttga caccctcatg aaaagtccta
     2521 taatccttgc gttcaagttg tgggtcgtta gctcaattgg tagagcatct gactcttaat
     2581 caggcggtta caggttcaag tcctgcacga cccaccacca cttgcccaaa gattcagttc
     2641 cctgcgatgt cagtaagtag tcagcgaggg tggcggaact ggcagacgcg ctggacttag
     2701 gatccagtgg gtaattccat gcgggttcga ctcccgccct tcgcaccaac ttggaggaga
     2761 ccgtggaata caccactacc aacagaaccg gcaattcgat cgttctctct gcaacattca
     2821 ccgccgacga agtctcggca atgcgccatg aagcgacgct ggctatggct ggcaagctgc
     2881 gtatacctgg gtttcgtccg ggcaaggcgc ctctctcgat cgtggcgaag tacgtcagcg
     2941 acgaggagct gaaggatgac gtcctccaga aggcggcctc tcagacgtat gtcacgttcc
     3001 tcaaggaaca caaggacaca gacgccctca tttttgagcc cgagatcgtt gaggcagccc
     3061 cggaagacag cccgactgac ggactgaagg tcgacattcg tgtcttcgag atgcccaagg
     3121 cagaccgtga attctggagc actgtagagg tcgaagacgt ccccgttgat accgccaggg
     3181 ctgttcagag ccgtatcaat gctcttgttg acgcggtgac cgagacgtcg ccgaaggatg
     3241 gtccagcggc ccagggcgat tccgtgacgg tcaccatttc gaccgagaga tcgaaagccc
     3301 cccatcacac ggagtttacg ctgggcgagg gtgaagtggg aaaggcctat gaaccgttcg
     3361 tcgtcgggat gaacgtcgga gaatcgaagc acttcagcgt ccagatgcag gagagcgctc
     3421 ttgagggaga agtcactctt gaaggtgtcg cgatgaagca tgtccccgag gtcaatgatg
     3481 acttcgctcg gaccgtcggc gcgttcgagt cacttgaaca gcttcggaag acccttgaag
     3541 aggacgaaca gcacaaggcc gacgaggcga gaaaagacgc ggtctttggc cgtgcgatcg
     3601 agaaggccgc agagaagttg ggcatcgact tccctgggta tgttctgcgt gacgccaccg
     3661 aagaacgact tggggaaatc aagaatagcc ttgcccgcaa tggcctttct ctcaacgaat
     3721 acctgaagta caatgacatc accgtcgaac agttgaccaa agacgtcgag gctgatgcag
     3781 tcaaactcct ccgacgggac ctcctgatgg aggctactga gagagcgtgc agcatagtgg
     3841 caggggacgc cgatatcgac gcgtactccg agtcacacaa agaggaactg gcaaaggcca
     3901 acgttgacac gacgacggag gatggtcgca agaccgtccg caatgtcatc gtctgggaca
     3961 atacgcgcag tctcattgtc agtgcagttc agctggcaat gaccccagag gggaagcggg
     4021 aggcacaatg agtgtcattc catatgtagt cgaacaaacg gcccatggcg aacgcggtta
     4081 cgatatctac tcgcggcttc tgaaggaccg gatcgtattc ctcggcggag agatcgacac
     4141 gcaggtctcg aacctgatcg tggcggagat gctctatctg acggcagaga acgcaaccaa
     4201 ggacatctac ctctacatca acagcgtcgg aggcgaggtc acgcccggca tggccatcta
     4261 tgacacgatg cagtacatca atgcgcccgt ttcgacgatc tgtctggggc aggctgcgag
     4321 tctcgctgca gtcctgctgg ccgccggccg caaaggaaag agattcgcgc tgcccaattc
     4381 gcgtatcatg atccaccagc cttggggcgg tgcgcagggg caggctaccg acattgagat
     4441 tcaggcgaag gagatcctcc gcatcaagga atctgtcagc agtattcttg cctcgcacac
     4501 ggggaaaccc ctggacgtcg tgcggcgaga caccgagaga gacttcttca tgtctgccga
     4561 agaggccgtg gactatggca tcgtcgatca ggtcatatcc aagattggtg tctccgggga
     4621 agtcggcttg tcggggaaga atgtccagtc acaatagcga agaaccatcc tgctcgttct
     4681 gcggcaagaa gtcgagcgag gttgccctgc ttgtcaaggg actcgatgga tcgtttatct
     4741 gcgacgactg catcgagcgg ggaagccagc tcatgcagga gtcgaagcac gagactcgtg
     4801 ctctcgactt caaggcactg cgtcccaagg agatctacgc tcgcctgaac gagtacgtca
     4861 tcgggcaagg cgaggtcaag aaggctctcg ctgtcggcgc gtacaatcac tacaagcgct
     4921 tgagggccag gaaggactcc aaggtcgaga tcgagaagtc caacatcctg ctcgcgggcc
     4981 ccacgggcgt cggcaagaca tatctcgcaa gaacgctcgc gcgcattctg gatgtgccct
     5041 tcacgatcgc cgatgcgacg acccttaccg aggctggcta cgtcggtgaa gacgtagaga
     5101 acatccttct ggggcttctg caggcaacca atttcgatgt cgcccgtgct gagtgcggga
     5161 tcgtatatat cgacgagatc gacaagatcg gacggaagtc cgagaaccca tccattacgc
     5221 gggacgtgag cggtgaaggg gtccagcagg cgctcctcaa gattgtggaa ggcactactg
     5281 ccaacgttcc tccccaaggt gggcggaagc atccgttaca ggagtacatc aagttcaaca
     5341 cgcaggacgt tttgttcatc gcaggcggga cgtttgacgg actcgatacc atcaggacac
     5401 ttcgagctac gcctcgggaa gtcgggtttg ttcacgaacc ggctgaccgg gaaggcagga
     5461 ggctgctgag ttcggacttc gtgaagttcg gcatgcttcc tgagcttgtt ggacggtttc
     5521 ctctgaaact cgaactggag gagctctccg tttcggactt gaagaggatt ctcgtcgagc
     5581 caaagaactc gatcgtccag cagtatcagg aactcttcgc gatggacggc gtggcactga
     5641 cgttcagcgg gtcggcattg gaacgcatag ccgagaaggc cagagacatg ggtatgggag
     5701 ctcgcggtct gaagtccgtt gtcgaccagc ccatggtgga tctcatgttt gagcttccct
     5761 cattcaagga gatcaccgaa gtgacggtgg gagacgactt cttctcgaat cgtcacgaga
     5821 tcatcgcgac gagatctgat gggtcgaccg aacgcattcc attgcaggag tctgcatgac
     5881 aggagagaac aacatccatg agtgaacgcg attgggagct ttctgaagcg ccgctgattc
     5941 cacttcggaa cgtggtggtg tttccatatc atgtccttcc cctctttgtg ggcagaccga
     6001 agtctctgaa gagcatcgaa tctgcattag cgggcaatcg caagatcgtg ctggtatgcc
     6061 agagagacga gtccaatgaa gatccggggc ttgctgacct ctacgacatc ggtgttggcg
     6121 cagagatcct gcagttgctc aagctgcccg atggtagcga tagagtactt gtcgaggggg
     6181 tccgcagggt tcgcatcaca aacgtacagt cggacagcga tgagatgctg gtggccactg
     6241 tagagccgct gccagccgac gagaagacct cgacggagcg ccaggcgctc atgcgagtcg
     6301 tccttgacca gttctcccag tacgtcgagt tgtccagaaa actgccatcg gagactgtga
     6361 acaatgtctc aggcgtcact gaacccgaca ggtttgccga ccttgtcgcc agtagtctcc
     6421 tgctcgggac tgaggaccgg caggctctgc ttgggacagt agatgcagaa gaacgcctcc
     6481 atcacatcgc agaactcctg gccaaggaga tctacgtgct ggagctcgcg cggaagatcg
     6541 aaagcgatgt gaaggcgaag gttgatcagt ctcaacggga atactacctc cgtgagcagg
     6601 t
//
LOCUS       DATMZD010000022         2194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_131670,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000022 DATMZD010000000
VERSION     DATMZD010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131670"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1236
                     /locus_tag="VN478_00490"
     CDS             <1..1236
                     /locus_tag="VN478_00490"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWQ21186.1"
                     /translation="HVDHTGSISFLDETIPLYATPVTAAVLRAVEETQFSSGPENGVT
                     DFILRPNLGANKHAQRDLVDLTGPVQFPDCRVTPFPVDHSIPGACAYLIEYDGGTLVY
                     TGDLRFHGRMGAITEDSIDQLAAAHPDAVLIEGTNLRSNDGSSDHDTAFHRHFKFWSE
                     QDVEVKACEEVQRTHGLVIADFGMKDLDRLMTFQRAARATGRKLAVLPRDAHIVRLAQ
                     EAGYPTIDLDDPDLVLYVKRAGSGTYDKSDIKQDWARSTLLRFSGADPECVDPKDRRD
                     AVEDVLHGSCPRVVRAVDVAAHQSDYLLSLGYWDIQELCDLHPAPGSLYIHSSAEAFN
                     EEMSWSQERLAKWLRLMDMDSVHIHASGHAPQDDLFRIVERLDPRHVYPVHTEHAESY
                     AERFGDKVGLVTNGQDVSL"
     gene            1362..>2194
                     /locus_tag="VN478_00495"
     CDS             1362..>2194
                     /locus_tag="VN478_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008180013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HWQ21187.1"
                     /translation="MIDENGFIKPDAGETQHLDTPDTVEGYVTPEAPPEASSEPDGIL
                     PQDYSTPSPEPIPAVPAYGSPSPRPQPAQQSNPGRVFLVWGLIIGLLLGSVVGGIAGN
                     YIVMKNPGTFPWARTVSTTNTGTTGSGITIVTTEEQAIENAVKIASPAVVQINITSVS
                     QNPFGFSSGTQEGLGSGFIITSDGYVLTNNHVVENATKITVVLKDGREFRGQVVGTDA
                     TSDVAVVKISGTNLPTVELGDSSTLTVGQRVIAIGNPYGLSQTVTTGVISALERNVQA
                     SA"
BASE COUNT          451 a          703 c          627 g          413 t
ORIGIN      
        1 catgtagacc ataccggctc gatctcgttt ctggacgaga ccatccccct gtacgccacg
       61 ccggtgacag ctgcggtcct gcgagccgtc gaggagacac aattcagctc cggcccggaa
      121 aatggcgtga ccgacttcat cctccgtccc aatcttggcg ccaacaagca cgctcagagg
      181 gacctggtcg acctcactgg tcccgtgcag ttcccggatt gccgggtgac gccattccct
      241 gtcgatcaca gtatccccgg agcgtgtgca tacctcatcg aatatgacgg agggacgctg
      301 gtgtacacgg gcgacctccg gttccatggc cgcatgggcg ccatcacaga ggacagtatc
      361 gaccagcttg cggcagccca cccggacgcc gttctgatcg aagggaccaa cctgcgttcg
      421 aatgacggat cctcagatca tgacacggca ttccaccgcc acttcaaatt ctggtccgag
      481 caggacgtcg aggtcaaagc gtgcgaggag gtccagagga cacacggcct cgtcatcgca
      541 gactttggca tgaaagatct ggatcggctc atgaccttcc agcgcgccgc tcgcgccaca
      601 ggacggaagc tggccgttct cccgcgtgac gcccacatcg tccggctggc gcaggaagcc
      661 ggctatccca ctatcgacct ggacgacccg gacctcgtgc tctacgtcaa gcgcgccggc
      721 agtggcacct acgacaagag cgacatcaag caggactggg cccggtcgac acttctccga
      781 ttctccggcg ccgatcctga gtgcgtggac ccaaaggacc ggcgagacgc cgtggaagat
      841 gttctacatg gatcatgccc tcgcgtcgtc agggctgtcg acgtagcggc ccatcagtcg
      901 gactacctgc tttcgctggg atactgggac atccaggagc tatgtgacct gcatcctgct
      961 cctggttcgc tatatatcca ttcgtcggcc gaggccttca acgaggagat gtcgtggagc
     1021 caggaaaggc ttgccaagtg gctgcggctc atggatatgg actccgtgca tattcacgct
     1081 tccggccacg ctccacagga tgacctattc cgcatcgtcg agagactcga tccgaggcac
     1141 gtgtatcctg tccacaccga gcacgccgaa agctatgctg aacggttcgg agacaaggtc
     1201 gggctcgtca cgaacggaca ggacgtctct ctgtagaacg gaagccgggg ccctggcatc
     1261 gtggcgattc catcacattc ctcgtaacag aaccggccac agttgcgtat atacaggtgt
     1321 atgtttcacg cagacacctg tttcaggtga ggaggtacga gatgatcgat gagaatggat
     1381 tcatcaagcc cgatgctgga gagactcagc atctggatac accagacacg gtagagggct
     1441 atgtgacccc ggaggctccc ccggaggctt cttctgagcc cgacgggatt ctgcctcaag
     1501 actacagtac tccgtcgcct gagcccatcc cggctgtccc ggcctatggt tccccttcgc
     1561 cccggcctca gccggctcag cagtcgaatc cgggccgcgt gttccttgtc tggggactca
     1621 tcatcggatt gctgctcggc agtgtcgtag gcggcatcgc aggcaactac atcgtcatga
     1681 agaacccagg cacgttccca tgggcgcgca cggtgtcgac cacgaataca ggaacgaccg
     1741 gctcgggtat taccatcgtc acgaccgaag aacaggccat cgagaacgct gtcaagatcg
     1801 cttcgcccgc cgtcgtccag atcaacatca cgtcggtcag ccagaacccg tttggcttct
     1861 cctcgggaac gcaggagggc ctcggctccg gcttcatcat tacgtctgac ggctacgtgc
     1921 tgacgaacaa tcacgtcgtg gagaatgcca ccaagatcac cgtcgtcctc aaggatgggc
     1981 gtgagttccg gggccaggtc gtcggcacgg acgccaccag cgacgtcgcc gtcgtcaaga
     2041 tcagcggcac caacctgcca actgtggagc ttggtgactc atccacgctc accgtcggcc
     2101 agagagtcat tgccataggc aacccctacg gtctgagcca gaccgtcacc accggcgtca
     2161 tttcggccct tgagcgaaac gttcaggcct cagc
//
LOCUS       DATMZD010000023         7417 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_141507,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000023 DATMZD010000000
VERSION     DATMZD010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7417
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141507"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..799)
                     /gene="purQ"
                     /locus_tag="VN478_00500"
     CDS             complement(83..799)
                     /gene="purQ"
                     /locus_tag="VN478_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HWQ21188.1"
                     /translation="MRFAVAVFPGSNCDVDAHRALHDVLGEDVAYLWHRETDLSAFDC
                     VVLPGGFSYGDYLRPGAIARFAPLMESMVRFARQGGMVLGICNGFQLLTEAGLLPGAL
                     LRNRDLTFVCDTVPVRVENADTAFTRRCRAGQVLRLPIAHGDGNYAVDAATLAGMEQR
                     GQVILRYCAPDGTVDEQHNPNGSAANVAGIVNEQGNVAGMMPHPERAAEELLGGTDGL
                     FILQSMVQELERRQHEACRR"
     gene            complement(796..1047)
                     /gene="purS"
                     /locus_tag="VN478_00505"
     CDS             complement(796..1047)
                     /gene="purS"
                     /locus_tag="VN478_00505"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HWQ21189.1"
                     /translation="MKARVLISPRKGIADPQGTAVEQALHALGFTGVRTVRVGKVIDL
                     ELPEGDRTEALAALTRMSEQLLANTVVEAYSCTILDEDT"
     gene            complement(1044..1760)
                     /gene="purC"
                     /locus_tag="VN478_00510"
     CDS             complement(1044..1760)
                     /gene="purC"
                     /locus_tag="VN478_00510"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HWQ21190.1"
                     /translation="MTRKELLYEGKAKRVYRTDDPGEYVIEFKDDATAFNGVKKGTIA
                     DKGVMNNEMSSYLFAMLAEAGVANHFIQPLSDREMLVRQVQIIPLEVIVRNIAAGSMS
                     KRLGIAEGTLLAEPVVEFSYKDDALGDPLVNEDHIRALRAATDAEVATLRKLALQVNR
                     LLQERFAAAGITLVDFKLEFGRADGAILLADEISPDTCRFWDSTTGKKLDKDRFRRDL
                     GGVEEGYHEVLGRLLTGRGQ"
     gene            complement(1757..2281)
                     /gene="purE"
                     /locus_tag="VN478_00515"
     CDS             complement(1757..2281)
                     /gene="purE"
                     /locus_tag="VN478_00515"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007049915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HWQ21191.1"
                     /translation="MPQIVVVMGSDTDLPVLEPAFAVLRSFPVTWEARVISAHRTPGA
                     AMEFARGACGAGVQVIIAAAGKAAHLAGALAASTTLPVIGIPISSPGLGGLDALLATV
                     QMPTGVPVATVGIDNAKNAALLALQIVGLRDARVAARLLENRQQMAEEVGHKDQTVRE
                     HYGSAVEIEQEGPA"
     gene            complement(2705..4708)
                     /gene="infB"
                     /locus_tag="VN478_00520"
     CDS             complement(2705..4708)
                     /gene="infB"
                     /locus_tag="VN478_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HWQ21192.1"
                     /translation="MKKVRIYELAKELNLKSARMLEILAELGVPSKGVVGTIEEETAN
                     LVKEIVDKEKAASSGVKKETPKAAKAAPKAAPEKEKEKPTPATVEPVVTVEKPKAFVE
                     SVTYAAFAKQFELKQHELNTMMLKNGIPVRECALLDPNDADYLARILGANYVEPSDKY
                     MKSFETRPPVVTVMGHVDHGKTTLLDFIRKSSVASREKGGITQKIGAYEVQVKGKTII
                     FIDTPGHEAFTSMRLKGSQVTDVVILVVAAEEGVKEQTLEALDHAKAAKVPIIVAMNK
                     IDKPEANPERVKQQLADRGLQPDDWGGDTVFLPVSAKTGKGVDELLDMILLQAEMAGL
                     KTRSSVDASGSVIEARVDKNIGVLTRLLVQTGTLHVGDDIRVGDCVGKIKRMTNEAGA
                     NLREAKALIPIEVMGLSELPEAGEVFHLVEDVDSCRMVIDEKRQKERLAFLAQEQKKP
                     LTLDDLFNEEEALEKKKLTLILKADALSTLDAVKFELKKVKTKTIPLEILHEGTGGIT
                     KSDVLLASASNAVIVGFNVVPLAEALKVAATEKVQIRTYDLIFELGDSIDKMIAGLTK
                     PVFEEVVQGRARIKDVFKITGVGAIAGCLVEDGKIIRGAKARVVRNAVVVYANSSISS
                     LKRFKEDAKEVLKGYECGMGLQNFNDFKVDDIIETLVMEEKKP"
     gene            complement(4686..5774)
                     /gene="nusA"
                     /locus_tag="VN478_00525"
     CDS             complement(4686..5774)
                     /gene="nusA"
                     /locus_tag="VN478_00525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HWQ21193.1"
                     /translation="MRDEIQSAIYELEKERGISRDYIVTAIKEAFEAAYQQQYQKDNF
                     LVKADTGTGLVRLYSRKTVVAKVVDSDSEISLKDARKVEPEAKTGQDVLIQVKIEQLP
                     LSVIVKARKFIDDKIKERENDLVFERFHSQVGTLVNGEVLRQNKEGLLINLARCEGIM
                     PKDQQIPGERLRFQGVAKALILSVDKSAQGPRVVLTRSHPDFIQRLVELEVPEVGSGL
                     VEIKRIVREPGQRAKISVFSRDPKIDPVGAVIGPMGGRIRAVSREIAFEHIDVIRYDP
                     NPAKYAINAFSPAKVLDAERRPEDGTFVVHATAEAFPVALGKGGVNVRLVERLLDAKV
                     ELVQVEEGQPQATPTREAGETHEESTDL"
     gene            complement(5764..6213)
                     /locus_tag="VN478_00530"
     CDS             complement(5764..6213)
                     /locus_tag="VN478_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21194.1"
                     /translation="MDKRFEDIVRLEVAKSNTTLVAIENAPGMVSVVLMTPGKDISVD
                     ELENVSRAISHSFIELYGDDYLPAFEVTSPGLDRVLKTARELELFAGRLVRMTTKESR
                     EPVIGTLGASDGSTLHVTVLGLDRTFDMRQVTKISLWDEILGGACEG"
     gene            complement(6427..>7417)
                     /locus_tag="VN478_00535"
     CDS             complement(6427..>7417)
                     /locus_tag="VN478_00535"
                     /inference="COORDINATES: protein motif:HMM:NF025930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21195.1"
                     /translation="ETLAGGSQGIRFGLLAIKNLGSKAIDEIMEERSNRPYTTFKDFQ
                     RRSGGSKVNRKSVECLIKSGAFDSLGDDRVKVLADFNNQNGKAAAPDDTGTGLFGGSV
                     SESAAPAIACDTAGAAAMEKEAFGFFCFCSPFQQYEPLLAKYKAKRVSECAELDDGTT
                     VTVMGLLTSAKTAKSKAGHAYGKCTLEDDSRKVELMVFEYVLSQFQPCFDREGAIVLE
                     ANVRMDGDELSLTAKKFIDFITPEQAKGSKPVAVVHRVLKLTMTEEQVNGETLKNVLL
                     LAHKHGGTEPWRILYRNSTDHCLEVGNKHWVTVTPALLKGLVGILGSGGVDYS"
BASE COUNT         1287 a         2394 c         2161 g         1575 t
ORIGIN      
        1 ctccaggtct ccgggctcgc gcccgacgag ttcgacgatg cgcgtgtact cggcgtccgt
       61 gagtccgacg cttgcagccc gttcagcgcc ggcaagcttc atgctgcctc ctttcgagtt
      121 cctggaccat ggactgcagg atgaacaggc cgtcggtccc gcccagcaac tcctccgccg
      181 ctcgctcggg atgcggcatc atgccggcca cgttgccctg ctcgttgacg atacccgcga
      241 cattcgctgc tgagccattc gggttgtgct gctcgtccac cgtgccgtcc ggcgcacagt
      301 agcgaaggat cacctgtccc ctctgctcca tgccggccag cgtagcagcg tccacggcat
      361 agttgccgtc gccgtgggca atgggcagcc tgagcacctg cccagcccgg cagcgccgag
      421 tgaacgcggt gtcagcgttt tcgacgcgca ccggcacagt gtcacagaca aaggtcaggt
      481 cgcgattgcg caacagagcg cccggcagca gccctgcttc ggtaagcagc tggaagccgt
      541 tgcagatgcc caggaccatg cctccctgcc tggcaaaccg gaccatagac tccatcagtg
      601 gagcaaaccg cgcgatggcg cccggtcgca ggtagtcacc atatgagaag ccgcccggca
      661 ggaccacaca gtcgaaagcg gacaggtcgg tctcgcgatg ccagagatac gcaacgtctt
      721 ctccgagtac gtcgtgaaga gcacggtgcg cgtccacgtc gcagttggag cctgggaaga
      781 ctgcaaccgc aaacctcatg tgtcctcgtc caggatggtg caggaatacg cttctacgac
      841 agtattggcg agaagctgtt cgctcatgcg cgtgagtgct gcaagcgctt ccgtgcggtc
      901 gccctcgggc agttccaggt cgatgacctt gcctacacga acagtgcgga cgccggtgaa
      961 cccgagtgcg tggagtgcct gttccacggc cgtcccctgg gggtctgcga tccccttcct
     1021 ggggctgatc agcacgcggg ccttcattgt cctctcccgg tcaacagacg cccaaggact
     1081 tcatggtagc cttcctcgac gccgcccagg tcgcgacgga agcggtcctt gtccagcttc
     1141 ttccctgtag tgctgtccca gaagcggcag gtgtccggcg agatctcgtc ggcaaggagg
     1201 atcgccccgt ctgcccgccc gaactccagc ttgaagtcga ccagagtgat gcctgctgcg
     1261 gcgaaccgtt cctgaagcag ccggttcact tgcagcgcca gcttccgaag ggtcgccact
     1321 tcggcgtcgg tcgccgcgcg cagtgcccgg atgtggtcct cattgacgag aggatccccc
     1381 agggcgtcgt ccttgtagct gaactccacg accggctctg caagcagtgt gccttctgcg
     1441 atacccagac gcttgctcat cgaccccgcg gcgatgttgc gcacgatcac ctcgagcggg
     1501 atgatctgca cctgccgaac aagcatctcc cggtcgctca aaggctggat gaaatgattg
     1561 gcgacgcccg cctcggcgag catggcaaac agatacgatg acatctcatt gttcatgacc
     1621 cctttgtccg cgatcgtccc cttcttgacg ccgttgaatg cggtagcgtc gtctttgaac
     1681 tcgatgacgt actctcccgg atcatcggta cggtagaccc tcttcgcctt gccttcgtac
     1741 aacagttctt ttcgtgtcat gctggaccct cctgttcgat ctcgactgcg ctgccgtagt
     1801 gctcacggac tgtctggtcc ttgtggccca cctcttccgc catttgttgc ctgttctcaa
     1861 gcagccgtgc cgccacccgc gcgtctcgca ggcccacaat ctgcagtgcc agcaaggctg
     1921 cgttcttcgc gttgtcgatg ccgacggtcg ctaccggtac gccggtcggc atctgcacgg
     1981 tagcaagcag ggcgtccagt ccgcccagac cgggcgagct gatcgggatg ccgatgaccg
     2041 gcagggtcgt ggatgcggca agtgcgcccg ccagatgagc ggctttccct gctgccgcaa
     2101 tgatcacctg caccccagcg ccgcaggcgc cccgcgcaaa ctccatggcc gccccgggcg
     2161 tacggtgcgc cgagatgacc cgcgcttccc aggtgacggg aaaggagcgg agcacggcaa
     2221 acgccggttc caggacgggc aggtctgtgt cactgcccat gacgacgaca atctgtggca
     2281 tggtggcctc ctcgatgaca tgtggtcacg aaatggaaaa accccggttg cctggattca
     2341 gggcagccgg ggtggtatcg cctggttggt atgaggtcgg agtcttctcc ccgaaggagg
     2401 gatagactcc actgtctcat ggcagtggta cctggaggac tcggtggtct tccagcaggc
     2461 gatgggacaa aggcggaatt cgggtgacgg tggtggtcgc tgattgtcgt agttcagcca
     2521 aggccatcga tcgttctccc agtgaacggg cggcctgata ctccacccgg aattgtgaca
     2581 gagatatcct agctgatgtc tggcagcctg tcaactccca cacgtccgcc agccatgaac
     2641 aagcgccggg catctatcgc ccggcgcgtt cgtttcggtt cagttggaca cggggtgtcc
     2701 tgccctacgg cttcttctcc tccatcacca gcgtttcgat gatgtcgtcg accttgaagt
     2761 cgttgaagtt ctgcaggccc atgccgcatt cgtagccctt gaggacttcc ttggcgtctt
     2821 ccttgaagcg cttcagcgag gagatgctgg agttggcata cacgacgacg gcgttgcgga
     2881 ccacgcgggc cttggcgccg cggatgatct tgccgtcctc gaccagacag ccggcgatgg
     2941 cgccgacgcc ggtgatcttg aacacgtcct tgatgcgggc gcgtccctgc accacttcct
     3001 cgaagacggg cttggtcagt ccagcgatca tcttgtcgat ggagtcgccg agttcgaaga
     3061 tcaggtcgta ggtgcggatc tggaccttct cggtcgcggc gaccttcagg gcttcggcca
     3121 gaggaaccac gttgaagccg acgatgacgg cgttgctggc ggaagccagc aggacgtcgg
     3181 acttcgtaat gccgccggta ccctcgtgca ggatctcgag cggaatggtc ttggtcttga
     3241 ccttcttcag ctcgaacttg acagcgtcca gcgtggagag ggcgtcggcc ttgaggatga
     3301 gagtcagctt cttcttctcg agcgcctctt cttcgttgaa caggtcgtcc agcgtcagcg
     3361 gcttcttctg ttcctgtgcc aggaacgcaa ggcgctcctt ctggcgcttc tcgtcgatga
     3421 ccatgcggca cgaatcgacg tcttccacca ggtggaagac ctcgccggcc tcgggcagct
     3481 ccgacaggcc catgacttcg atgggtatga gcgccttcgc ttcacgaagg ttcgcgcccg
     3541 cctcgttggt catgcgcttg atcttgccca cgcagtcgcc gacacggatg tcgtcgccga
     3601 cgtggagcgt gccggtctgg accaggagac gagtcaggac gccgatgttc ttgtcgacgc
     3661 gcgcctcaat gacagagccg ctcgcatcga cggaggaccg ggtcttgagt cccgccatct
     3721 ccgcctgcag gaggatcatg tccagcaatt cgtccacacc cttgccggtc ttggccgaca
     3781 caggaaggaa gacggtgtct ccgccccagt cgtcaggctg cagaccacgg tccgcgagct
     3841 gctgcttgac gcgctcgggg tttgcctcgg gcttgtcgat cttgttcatg gcgacgatga
     3901 tcggtacctt ggccgccttc gcatggtcga gagcctcgag ggtctgttcc ttgactccct
     3961 cttcggcggc gacgaccagg atgaccacgt cggtgacctg cgaacccttg agacgcatcg
     4021 acgtgaacgc ctcgtggccg ggcgtatcga tgaagatgat ggtctttccc ttgacctgaa
     4081 cttcataagc gccgatcttc tgggtgatgc cacccttctc ccggctggcg acgctgctct
     4141 tacggatgaa gtccagcagt gtggtcttgc catgatcgac gtggcccatg acggtcacga
     4201 caggagggcg cgtctcgaag gacttcatgt acttgtccga tggctcgaca tagttcgcgc
     4261 cgaggatgcg ggcaaggtag tccgcgtcgt tggggtccag caaggcgcac tcgcgcacgg
     4321 gaataccgtt cttcagcatc atcgtgttga gctcgtgctg cttgagctca aactgcttgg
     4381 caaaagccgc atacgtgaca gactcgacaa atgccttggg cttctctaca gtgacaacag
     4441 gttcgaccgt ggcgggcgta ggcttctcct tctccttttc aggcgcggcc ttgggagccg
     4501 ccttggctgc cttcggagtc tctttcttga cccccgacga ggcggccttc tccttgtcga
     4561 cgatctcctt gacaaggttt gccgtttctt cttcaattgt gccgaccacg cccttgctcg
     4621 ggacccccag ctcagccagg atctccagca tgcgtgccga cttcagattc agttccttag
     4681 cgagctcata gatccgtact ttcttcatgc gtctcacctg cctcacgggt cggggtcgct
     4741 tgtggctgcc cctcctctac ctgaacgagt tccacttttg cgtccaatag ccgttctacg
     4801 agccggacgt tgaccccacc cttacccagg gcgacgggaa acgcctctgc cgtggcgtgc
     4861 accacaaagg tgccgtcctc cggcctgcgt tctgcatcca gcaccttggc agggctgaaa
     4921 gcgttgattg cgtattttgc gggattcgga tcgtaccgga tgacgtcgat gtgctcaaaa
     4981 gcgatctcac gcgataccgc ccggatgcgc cctcccatcg gaccgatgac tgctcccacg
     5041 ggatcgatct tcggatcgcg gctgaagacg gagatcttgg cgcgctgtcc cggctcacgg
     5101 acgatccgct tgatctcgac gaggcccgaa ccgacctcgg ggacctccag ctccacgaga
     5161 cgctggatga agtccggatg cgaccgcgtc aggaccacac gcggcccctg cgccgacttg
     5221 tcgaccgaca ggataagcgc cttggcgaca ccctgaaagc gaagccgctc cccggggatc
     5281 tgctggtcct ttggcatgat cccttcgcac cgggcaaggt tgatgagcaa tccttccttg
     5341 ttctggcgca ggacttcgcc gttgaccagc gttcccacct gtgagtggaa ccgctcgaag
     5401 accaggtcgt tctcgcgttc cttgatcttg tcgtcgatga acttgcgtgc cttcacgatc
     5461 accgacaggg gcagctgctc gatcttgacc tgaataagca cgtcctgccc agtcttcgcc
     5521 tccggctcca ccttgcgggc gtccttcagt gagatctcgc tgtcgctgtc gacgaccttt
     5581 gccaccaccg tcttgcgcga atagagccgc acaaggccgg tacccgtgtc agccttcacc
     5641 aggaagttgt ctttctggta ctgctgctgg tatgctgcct caaaagcttc cttgatcgcc
     5701 gtcacgatgt agtccctgga gattccccgt tccttctcca gctcgtagat ggcactctgg
     5761 atctcatccc tcacatgcac ctcccagtat ctcgtcccac aacgagatct tcgtcacctg
     5821 ccgcatatcg aacgtgcggt ccaagccaag caccgtcacg tgcagcgtac taccgtccga
     5881 tgctcccagg gtcccgatca ccggttccct ggactccttc gtcgtcatgc gcacgagcct
     5941 gccggcaaac agttccagtt cccgcgccgt cttgagaacg cggtccaatc cggggcttgt
     6001 cacctcgaac gctggcaggt agtcgtcgcc atacaactcg atgaacgaat ggctgatcgc
     6061 ccggctgacg ttctcgagct cgtcgaccga gatgtccttc cccggcgtca tgagcacgac
     6121 actcaccatg ccaggcgcgt tttcgatagc gacaagcgtc gtattgctct tcgccacttc
     6181 cagccgcact atgtcctcga atctcttgtc catacgtaca ctccatgtga aaagaaaaag
     6241 tggcccgctc gcgcgcccac ttttcgtctt ccaccaatat gaaacgtccg aaagcagggg
     6301 cttccggacg tcatgccgct cagttccttg tcacgttcat gacattacga agaatagtat
     6361 acctgtgaat ggccaaatgg caacctcaat ttcctttaca acttgacgat accttcgaac
     6421 cagccgctac gagtagtcca cgccgccact gccgaggatc ccgacaagac ccttcaggag
     6481 tgccggagtg acggtgaccc agtgcttgtt gccgacctcc aggcaatggt cggtcgaatt
     6541 ccggtacagg atgcgccagg gttccgttcc accatgcttg tgtgcaagga ggaggacgtt
     6601 cttgagggtc tccccgttca cctgttcctc ggtcatggtg agcttgagca ctcgatgcac
     6661 cacggcgacc ggcttggacc ccttggcctg ttcgggcgtg atgaagtcga tgaacttctt
     6721 cgccgtcagc gacagctcgt caccgtccat ccgcacgttc gcctccagga cgatggcgcc
     6781 ctcgcggtcg aagcacggct ggaactggct gagcacatac tcgaacacca tcagttccac
     6841 cttgcggcta tcgtcttcga gcgtgcactt gccgtaggcg tggccggcct tggacttggc
     6901 ggtcttcgcg gacgtcagca ggcccatgac cgtgaccgtg gtcccgtcgt cgagttccgc
     6961 acactctgag acacgcttcg ccttgtactt ggccaggaga ggctcgtact gctggaaggg
     7021 actgcagaag cagaagaacc caaatgcctc cttctccatc gccgccgcac ccgcggtgtc
     7081 gcatgcgatg gcgggagctg ccgactcgct caccgagccg ccgaacaggc ccgtcccggt
     7141 atcgtcgggg gctgcggcct tgccgttctg gttgttgaag tcggcaagga ccttcacgcg
     7201 gtcatcgccc aggctgtcga acgcgccgga cttgatgagg cactcgacgg acttgcgatt
     7261 gaccttgctg ccgccggagc gccgctggaa gtccttgaac gtcgtatagg ggcggttcga
     7321 acgctcttcc atgatctcgt cgatcgcctt cgagcccagg ttcttgatgg caagcaagcc
     7381 gaaccggatg ccctgagagc cgccagccag cgtctct
//
LOCUS       DATMZD010000024         2191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_121622,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000024 DATMZD010000000
VERSION     DATMZD010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121622"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(335..1681)
                     /locus_tag="VN478_00540"
     CDS             complement(335..1681)
                     /locus_tag="VN478_00540"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HWQ21196.1"
                     /translation="MRISVVGTGYVGLTTGVCLAEKGHSVICLDKDAAKVARLQKGEC
                     PIYEHGLEPMMQRNMARGRLEFGDNAARIQGSDVVFIAVGTPEGSDGSPDVTAVMSAA
                     EECAPYLNDGGVLVVKSTVPPMTWRDVARCVAQARSSADAKGDVHVAVNPEFLKQGTA
                     VEDTMHPARVIIGVDDAVARAALLDLYADWSVPIVITDPVSAMLIKYGSNCFLATKIS
                     FVNEMANLCDVIGADIDDVALGMGLDPRIGPKFLQAGLGYGGSCFPKDTTGLLSVASA
                     AGTRMGILQATKTANEGQVAIAVSKLERVIGPVKGQTVAIFGVAFKAGTDDLRESRGV
                     ALARELLSRGGAVTGYDPVVFGKAVHLPFPLLNDPISCATGASAVIIATDSEAMTTLD
                     WSRIASVMRQRVILDGRNILDPEAVAEAGMTYISMGRRGVVAPCDQPAGVQGRQTT"
     gene            complement(1678..>2191)
                     /locus_tag="VN478_00545"
     CDS             complement(1678..>2191)
                     /locus_tag="VN478_00545"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HWQ21197.1"
                     /translation="RILRVHGARRKYFSEVIGVNSRLDELQAAVLRVKLRHVDSWIAH
                     KRSLALAYDEGLRAFPDFVQPPIEAAGNVHTYHQYVIRVKRGRRDGLQRRLADEGIAS
                     AVYYPQLLHLQQCFASLEYRVGDMPRAEQALGEILSLPLWSEMPSEQVRVVTGAMGAY
                     FGELPSGECV"
BASE COUNT          427 a          733 c          605 g          426 t
ORIGIN      
        1 gccaccaggc tgcggagaaa ggagagctcc acggctggag agccagccat tcgtcccagc
       61 ggtcccattc ggactgtccg acttctcgaa cggtcaggtt catcatgcgt ctattcgcct
      121 tgccgtgtcc gaccgcatgg acccagggca tggtgccaac gattgctcca gaacgcggac
      181 acatgtggca cacaacccca ttgcgacacc ggcttccgcc cggagaaatc agacagtccc
      241 gtcagtcccg aactctctgg cacgctccat actgatgccg acctcgctct gaactccagg
      301 ggcgactgaa tgtgccgcat gacgatcctc acctctatgt cgtctgcctt ccctgaacac
      361 cagcgggctg gtcgcaggga gccaccactc cccttcggcc catggatatg tacgtcatcc
      421 cagcctccgc gacagcttcg gggtcaagga tattgcggcc atccagaatg acccgctggc
      481 gcatgacact ggcaatacgg ctccaatcga gggtcgtcat ggcttcgctg tccgtggcaa
      541 tgatcacagc gctcgcccct gtcgcgcacg aaatcggatc gttcagaagg ggaaacggca
      601 ggtggactgc ttttccgaac acgacggggt catatccggt cactgccccg ccgcgtgaaa
      661 gcagctcacg cgcgagtgct acgcccctgc tctctctgag atcgtcggtt ccggccttga
      721 atgccacacc gaagatcgca acggtctggc ctttgaccgg gccaatcacc cgctcaagct
      781 tcgagacggc gatcgccact tgtccttcat tggctgtctt ggtggcctgc aggataccca
      841 tccgggttcc agccgcactc gcgaccgaca ggagacctgt cgtgtccttt ggaaagcatg
      901 aacctccata tccgaggccc gcctggagga acttggggcc gatccgcggg tccaatccca
      961 ttcccagggc aacatcgtcg atgtccgcac cgatgacgtc acagaggttc gccatctcat
     1021 tgacgaagct gatcttcgtt gcaaggaaac agtttgagcc atatttgatg agcatcgcgc
     1081 tcaccggatc cgtgatgacg atcggcacag accagtccgc gtacaggtcg agcagagctg
     1141 ccctggcgac cgcatcgtct acaccaatga tcacgcgtgc cggatgcatc gtgtcttcca
     1201 cggccgtgcc ctgcttgagg aactcggggt tcacggcgac gtggacatcg cccttagcat
     1261 cagccgagga cctggcctgt gccacgcagc gggcaacgtc gcgccaggtc atgggtggca
     1321 cagtgctctt cacgaccagg acgccgccat cattgagata tggggcacac tcctccgcag
     1381 ccgacatgac ggctgtcaca tctggagagc catccgatcc ctccggggtt cccaccgcga
     1441 tgaagacaac atcgctgccc tggatacgtg cggcgttgtc accgaactca agccggcccc
     1501 tggccatgtt cctctgcatc attggctcca ggccatgctc gtagatgggg cactctccct
     1561 tctgaagacg tgcgaccttg gctgcgtcct tgtcaagaca tatcactgag tgtcccttct
     1621 cggcaaggca tacccccgtc gtgagcccca cgtaccccgt ccccacaacg ctgatcctca
     1681 tacgcactct ccactcggaa gctctccaaa atacgcgccc atcgcccctg tgacgacgcg
     1741 tacctgctca ctgggcattt cggaccacag tggaaggctc aggatctctc cgagcgcctg
     1801 ctcggcgcgc ggcatgtcgc cgacacgata ttcaagcgag gcaaaacact gctgcaagtg
     1861 cagtagctgc gggtagtaga cggcagaggc gatgccctcg tctgccagac gcctctgcag
     1921 gccgtcacgg cgtccacgct tgacccgtat gacgtattgg tgatacgtgt gtacgtttcc
     1981 ggcagcctcg atgggaggct ggacaaagtc cgggaacgca cgcagaccct catcgtacgc
     2041 cagcgccagg ctgcgcttgt gagcgatcca cgagtctacg tggcggagct tcactctcag
     2101 gacagctgcc tgcagctcgt ccagacggct gttgacacca atcacttcgc tgaagtactt
     2161 cctgcgggca ccgtgcaccc tgaggatcct c
//
LOCUS       DATMZD010000025         5558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_131765,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000025 DATMZD010000000
VERSION     DATMZD010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5558
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131765"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1082
                     /gene="yvcK"
                     /locus_tag="VN478_00550"
     CDS             <1..1082
                     /gene="yvcK"
                     /locus_tag="VN478_00550"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HWQ21198.1"
                     /translation="IAILVSVGLMVVAATLLARSVLTAVTGPVPRESVARTIYRYRMA
                     ESLPRVVVIGGGTGIKPIIESLRTENVKLTTIVTMADSGGSTGRLRDTTGMLPPGDFR
                     NALIAMAGESTRGARLLAYRFPEQLEGIGGHNIGNLMIAALSDIKGNFGDAVQELSDL
                     MQLPGRVLPMSLDSISLRAHFTDGTTVDGEDQVPLQGKSIDTIEVLPPHAKPFVPALE
                     ALSQADYIVIGPGSLFTSLIPPLSIEATADTIMRSAATKVLVVNAMTERGETDGYTVG
                     MHLHKIEEVLGRSCIDMILLSSTPIPSETLGRYAAAGVSPVFNDLARSQHPRVIEADI
                     CSTADGLIRHDPGKLRIVLRSLLGL"
     gene            1098..1961
                     /gene="whiA"
                     /locus_tag="VN478_00555"
     CDS             1098..1961
                     /gene="whiA"
                     /locus_tag="VN478_00555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HWQ21199.1"
                     /translation="MEFLKQELAGLPSTDPLLELSGALYGGSSLVLHHGGALSISFTS
                     KSPSIMRYVLSLSSHALISWEPGQALLSRVGHKLSFSVDLTPAQTHQLFSGLSPLEPV
                     RLERRLRGKHAAAAFTKGFFLISGYAAVQGTHLEFSCSLPVGRRLVERSLTSLQVTHG
                     SQIRRSSEITYLKSRQDIMALLGTWGASSSLMSLEELQLEREVDNQVNRSVNAELGNL
                     ERETIAVDELRAAFDRTDRTCLSPRTIEVVQARLRYPDLPLSQLAGKIPGAVSKSTIH
                     YHIDKVLRNAR"
     gene            1951..3210
                     /locus_tag="VN478_00560"
     CDS             1951..3210
                     /locus_tag="VN478_00560"
                     /inference="COORDINATES: protein motif:HMM:NF016827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21200.1"
                     /translation="MRDSQKQRQQLSLRQRSYLATRGLILEQPLSDFGTLMDRFAAGS
                     IVAEKHAMADPTGDSWNGGLDGFADSAESTPAEMIVDELLDRDLISDCQAECARFVAS
                     DLDEHGFFTRQIPRYAVSIGAPIADVRCVLSAIRTLEPAGLAATSVAHAYALQMSRIL
                     PELPVHACTLLLRPRQRSVSPGVLRACLAKLGVTCDTATFQRVLKALDPEPAKRMASG
                     QPRIIAPDIAIEQDPLTENLVCSVPEPIWRLAIDPTVVSSARKDPDARQQILAEVTRV
                     RWIEDAIMERTAMLARLGDALIASLAPYFAGQSSHPSRVPVEQLMQVTGMSRTVMVRA
                     LSRKYVKTPRGTFRLRSLVMDRWQTAAEPAREAVALLLQPHADARAMSDREIAEQLAR
                     QGIHVSRRTVAKYRLSLGIPARYFRDN"
     gene            3308..4315
                     /gene="gap"
                     /locus_tag="VN478_00565"
     CDS             3308..4315
                     /gene="gap"
                     /locus_tag="VN478_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HWQ21201.1"
                     /translation="MAKIAINGLGRVGRQVLKHLITRYPELEIVAANDITDEKTLAHL
                     LKYDSNYGTWDIDIEGKEGVIVIGGREVRIFREKDPAALPWATLGVDIVIESTGVFTD
                     ADKARGHITAGAKKVLITAPAKGEDITIVLGVNEDLLDVSKHTIISNASCTTNSLAPV
                     VKVLNDNWGVDKGYMTTVHSYTLDQRILDAPHKDLRRARNAATNIIPTTTGASKAVAL
                     VIPDMKGRLDGISLRVPTPTVSITVFDAVLAKPATREEINAALKLASETTMKGFLGYN
                     ELPLVSSDYRGTIYSGIVDGLSTKSSGTLVQVLSWYDNEWGYSARVADLTKLIVKKAS
                     Y"
     gene            4327..5517
                     /locus_tag="VN478_00570"
     CDS             4327..5517
                     /locus_tag="VN478_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HWQ21202.1"
                     /translation="MTKRSIRDLDVQNKRVLLRVDYNVPMSKDGAITDDKRILETLPT
                     IRYLLDKNATVIIVTHMGRPDGRIVESLRLDKIAQRLSALLGRPVDKLNSVVGADVTE
                     AVTNAKPGSIIMLENVRFLKEEKNGSEELAKQLAALADVYVDDAFATAHRPDTSVAGV
                     PQFLPSAAGFLLEREINVLETVTTSATKPFIAILGGAKVSDKIGLIKNLLTKTDAILI
                     GGGMAFTFLKAMGYGVGFSLVEEDYLKVADEIIEAAKTKGTRLLLPVDVIVTNEIKAG
                     SSHRIVDIEEIPADQIGVDIGPDTVEAFKAEITKANTIIWNGPMGVIEIPEFAEGTKS
                     LARIIAARSDALTVAGGGETASVIDALNLQSDFKHVSTGGGAFLEYLEGKQLPGIEAI
                     DSED"
BASE COUNT         1215 a         1697 c         1563 g         1083 t
ORIGIN      
        1 gcattgcaat actggtctcc gtcggactca tggtcgtggc ggccaccctt ctggcacgct
       61 ctgtgctgac tgctgtcacc ggacccgtgc ccagggagtc tgtcgcacga actatctacc
      121 gctaccggat ggcggagtca cttcctcgag ttgtcgtgat cggcggggga accggcatca
      181 agcccattat cgagtcactg cgaaccgaga acgtcaaact cacgactatc gtcacgatgg
      241 cagacagtgg gggaagcacc ggtcgccttc gcgacacgac cgggatgctg cctccgggcg
      301 acttcaggaa tgctctcatc gccatggcgg gtgagtcgac ccgcggcgcc aggctcctgg
      361 cttatcgttt ccccgagcaa ctcgaaggaa tcgggggtca caacataggg aatctcatga
      421 tcgctgcact cagcgacatc aagggcaact tcggcgatgc agtgcaggaa ctgtcagatc
      481 tcatgcagct gcccgggagg gtcctgccga tgtcgctgga cagcatctcg ctcagggcac
      541 atttcaccga cggtaccaca gtcgacggcg aagaccaggt ccccctccag ggtaagtcaa
      601 ttgacaccat cgaggtacta ccacctcatg ccaagccctt cgtccccgca ctcgaggcgc
      661 tgtctcaggc ggactacatt gtcattggtc ccggctccct cttcacaagc ctgataccgc
      721 cgctgtccat cgaggcgact gcagacacga tcatgcgttc tgctgctacg aaggtcctgg
      781 tcgtgaacgc gatgactgag cggggagaga cagacgggta taccgtggga atgcacctcc
      841 acaagattga agaggtcctt gggcgttcat gcatcgacat gatcctgttg agcagtactc
      901 ccatcccatc cgagacgcta ggaaggtatg ccgccgcggg cgtaagtccc gtcttcaatg
      961 acctcgcgcg aagtcagcat ccacgtgtca tcgaggccga tatctgctca accgccgacg
     1021 gcctcattcg acacgatccg ggcaagcttc gcatcgttct gagatctctc ctcggattgt
     1081 agacacggat ggcgacgatg gagtttctga aacaggaact ggcggggctg ccctcgaccg
     1141 accccttgct tgagctgtct ggagccctgt acggagggag ctccctggtc cttcaccacg
     1201 ggggagcgct gtccatctcg ttcacgtcaa agagtccatc catcatgcgg tacgtgctgt
     1261 ctctctcgtc gcatgcactc atcagctggg aacctgggca ggcactgctc tcaagagtcg
     1321 gccacaagct gagtttcagc gttgatctga ctcctgcaca gacgcatcag ctgttctcgg
     1381 gactctctcc gctggaacct gtcaggctcg agcggcgttt gcgggggaaa catgcagcgg
     1441 cggcattcac aaaggggttc ttccttatct ccggatacgc cgccgtgcag gggacgcacc
     1501 tggagttcag ctgttccctt cccgtcggac gtcgcctcgt agagcggtct ctcacgtctc
     1561 ttcaggtgac acatggctca caaatccgcc gctcctctga gattacctat ctcaagtccc
     1621 gccaggacat catggctctt ctgggcacct ggggagcctc aagctccctg atgtccctcg
     1681 aagagctgca actggagaga gaagtggaca accaggtcaa ccggtcggtc aacgccgagc
     1741 tcggaaacct ggagcgagag acgatagccg tcgacgagtt gcgagccgca ttcgatcgaa
     1801 ccgaccgaac gtgtctttct ccccgcacga tcgaggttgt ccaggcacga ctgagatacc
     1861 ccgatcttcc cctgtcgcag ctcgcaggaa agataccagg agctgtctcc aagagtacga
     1921 tccactacca catagacaag gtcctcagaa atgcgcgata gccagaagca gcggcaacag
     1981 ctcagccttc gtcagaggtc ctacctggcg acacgcggct tgattctgga acagcctctg
     2041 agcgatttcg ggactctcat ggatcgcttc gccgcaggca gcatcgtcgc cgaaaagcat
     2101 gccatggccg atccaacagg cgacagctgg aatggcggac tcgacggctt cgcagactct
     2161 gcagaatcca ctcctgcaga gatgatcgtt gacgagctcc tcgatagaga cctcatctca
     2221 gactgtcagg ccgagtgcgc gcgattcgtt gcctcggacc ttgatgagca tggcttcttc
     2281 accaggcaaa tcccgcgcta tgccgtctca atcggcgcgc ctatcgcaga cgttcgatgc
     2341 gtactgagcg ccatcaggac ccttgagccg gcagggctcg ccgccactag cgtcgcccat
     2401 gcatatgccc tgcagatgtc tcgcatactc cctgaattgc ctgtgcatgc gtgcacgttg
     2461 ctcctgcgtc cccgacagcg ctcggtctcc ccaggtgttc ttcgcgcatg cctcgcgaag
     2521 ctgggggtca cgtgcgacac cgccacgttt cagcgagtcc tgaaggcgct tgatcccgaa
     2581 cctgcaaagc gcatggcgtc cgggcaacca cggatcatcg ctcccgacat cgccatcgag
     2641 caggatccgt tgaccgagaa cctcgtatgc tctgtcccag agccgatatg gaggctcgca
     2701 atcgatccca cagtggtttc gtcagcccgc aaggaccctg atgcaaggca acaaatcctc
     2761 gccgaggtca ccagagtccg gtggatagag gacgcaatca tggaacgcac tgccatgctt
     2821 gcccgcttgg gcgatgccct catcgcgtca ctcgctccgt acttcgccgg ccagtcgagt
     2881 cacccatcac gtgtcccggt cgaacaactg atgcaggtga ccggcatgtc gagaacggtc
     2941 atggtacgcg ctctctcccg caaatatgtg aagacccctc gtggcacctt cagactgcgc
     3001 tcactggtca tggaccggtg gcaaacggcg gcagaaccag ccagagaggc tgtggcacta
     3061 ctgcttcagc cacacgccga cgcaagagct atgagcgacc gggagattgc agagcagctt
     3121 gcacgccagg gcatccatgt cagccgtcga acggtagcga agtatcgcct gtcactcgga
     3181 atccccgctc ggtacttcag ggacaattaa taaccttccg ttttcttttt agcggcattt
     3241 gcgtatcatg tgtgtacgtt gccgttccgg ctcaaccggc gcggcctatt tcttgtggag
     3301 gtagatcatg gctaagattg cgatcaacgg acttggcaga gtaggccgcc aggtattgaa
     3361 gcacctcatc acgcgctatc cagaactcga gattgttgct gccaacgaca taacggacga
     3421 gaagacgctg gcgcaccttc tgaaatatga ctcgaactac ggcacgtggg acatcgacat
     3481 tgagggaaag gaaggcgtga tcgtcattgg gggccgcgaa gtcaggatct tccgggagaa
     3541 ggaccctgcc gctctgccgt gggccacgct gggcgtcgat atcgtcatag aatcaacggg
     3601 agtcttcacc gacgccgaca aggcccgcgg acacataacc gctggggcca agaaggttct
     3661 gatcacggct cctgcaaagg gagaagacat caccatcgtt ctgggcgtca acgaggacct
     3721 gctcgacgtg tcaaagcaca caatcatctc caatgcctcg tgcacgacca actcactggc
     3781 tcccgtggtc aaggtgctca acgacaactg gggcgtcgac aagggctaca tgaccaccgt
     3841 ccacagctac acgctcgatc agcggattct cgatgcacca cacaaggacc tccgccgcgc
     3901 ccgcaatgct gccacgaaca tcattcccac gacgacaggg gcgagcaagg ccgtagcgct
     3961 ggtcatcccg gacatgaagg gaagactgga tggtatctcc ctgcgtgttc ccacgcccac
     4021 tgtctcgatc accgtcttcg acgcagtcct cgccaagccc gccacgagag aggagatcaa
     4081 cgcggcactg aaactggctt ctgagactac catgaagggg ttcctcgggt acaacgaact
     4141 gcctctcgtc tcgtctgact accggggcac gatctactcg ggcattgtcg acggcctgtc
     4201 caccaagtcg agcggaacgc tcgtgcaggt cttgagctgg tacgacaacg agtggggcta
     4261 cagtgcaagg gtcgccgacc tcacgaaact catcgtcaag aaggcatcct actaggaggt
     4321 attgtcatga cgaagcggag tatcagggac ctggacgttc agaacaagag agtactcctg
     4381 agagtcgact acaatgtccc catgtcgaaa gatggcgcta tcactgacga caagcgcatc
     4441 ctcgagacac tacctacgat tcggtatctg ctcgacaaga acgctactgt catcatcgta
     4501 acacatatgg ggcgccccga tggaagaatt gtggagtcgc tccggctgga caagatagcg
     4561 cagcgactgt cggctctcct gggcagaccc gttgacaagc tcaactctgt cgtgggcgcg
     4621 gatgtcacgg aagccgtgac gaacgccaag ccaggcagca tcatcatgct cgagaacgtt
     4681 cgcttcctca aagaggagaa gaacggttcc gaggagctcg caaagcagct tgctgctctc
     4741 gctgacgtct atgtggacga tgcgtttgcc actgctcaca ggcccgatac ctcagtcgcc
     4801 ggcgtaccac agtttcttcc atcagcagcc ggcttcctcc tcgagagaga gatcaacgtg
     4861 ctcgagactg tcacgacgtc tgccacgaag cccttcattg ctatcctggg tggcgccaag
     4921 gtgtcggaca agatcggact gatcaagaac ctcctcacca aaaccgacgc aatccttatc
     4981 ggaggcggca tggcgttcac gttcctgaag gccatgggat acggcgtcgg gttttctctg
     5041 gtcgaagaag actacctgaa agtagccgac gagatcatcg aggcggcgaa gacgaagggt
     5101 acccgccttc tccttccggt ggacgtcatt gtcacgaacg agatcaaggc aggaagttct
     5161 caccgcatcg tcgacatcga agagatccct gcggaccaga taggcgtaga catcggccca
     5221 gacacagtcg aagcattcaa ggcagagatc acgaaagcca atacgatcat ctggaatgga
     5281 ccaatggggg ttatcgagat ccccgaattc gcagagggta caaagtccct ggcacggatc
     5341 attgcagcaa ggtcggatgc gctgaccgtc gccggcgggg gtgagactgc ctcggtcatt
     5401 gacgctctca acctgcagag cgacttcaag cacgtgtcta ccggcggcgg agcattcctt
     5461 gaataccttg aaggcaagca gcttcccggc atcgaggcca tcgactcgga ggactagcac
     5521 gctttgggac ttgaaatcgg agcggctttc cgtataat
//
LOCUS       DATMZD010000026         3216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_40857,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000026 DATMZD010000000
VERSION     DATMZD010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40857"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="VN478_00575"
     CDS             complement(<1..395)
                     /locus_tag="VN478_00575"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HWQ21203.1"
                     /translation="MYEVVLLRHGESTWNKENRFTGWTDVDLSDKGVAEAHDAGKLLK
                     EGGFTFDLAYTSVLKRAIRTLWIVLDDMDLMWIPQVRSWRLNERHYGALQGLNKAETA
                     EKYGDAQVKLWRRSYVTQPPALVKSDERWP"
     gene            complement(483..1013)
                     /locus_tag="VN478_00580"
     CDS             complement(483..1013)
                     /locus_tag="VN478_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HWQ21204.1"
                     /translation="MTRRFSVRTLTTLGLLLAIGLLLPIVFHALFGAPGGKTLLPMHY
                     AVLLGGLLLGPVAGAFLGIATPLLSTLMTGMPAVAILPPMVVELAAYGLVAGLARRRW
                     RLAALWSLLLAMAAGRVALGLAVALLGPSIGLKAEPAAYVLGAIVTGLPGIAVQVILI
                     PLVLARGRFERSVEDR"
     gene            complement(1190..1834)
                     /locus_tag="VN478_00585"
     CDS             complement(1190..1834)
                     /locus_tag="VN478_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21205.1"
                     /translation="MNNNGQNERNTRALGLLLLAVGAWILIARFIHINLGDWLWPFWI
                     IVPGGLIIALGFHESRRKGEGLVTFGSIVAVTGIILFIQNITGQWQSWAYAWALLFPG
                     SIGLGKYLWGKQTGNAAAVHAAEKTIRVAVMLFIGFGIFFEVVLGIGGFHLGSAGRII
                     VPVLLIAAGVAIYLTSGTDGHIGIFQPPAPPQAMSAPPQPPVKQEGPEDDITRR"
     gene            complement(1944..2480)
                     /locus_tag="VN478_00590"
     CDS             complement(1944..2480)
                     /locus_tag="VN478_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21206.1"
                     /translation="MQVAGRQLKVLVVYYSSAGHTRTIAEQVAKAAQADLEELRPAGE
                     PSGMGTRHYLWTLRVLLLSQRPLLLPPEHDASTYDLIILGSPVLLGTFSPALRSYIRT
                     APLARKPVALFCSYRNSPGTAFKHVARMLSTSTVMAATLSVLDPVAAGPSSISIRAQR
                     WAHDLESAMAIQESRTRL"
     gene            2688..2894
                     /locus_tag="VN478_00595"
     CDS             2688..2894
                     /locus_tag="VN478_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020519863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HWQ21207.1"
                     /translation="MLTGKVKWFNAEKGYGFITRDDGEKDVFVHYSAIIGSGFKSLKE
                     GDLVEFEVVQGDKGPQAKDVQTKV"
     gene            3015..>3216
                     /locus_tag="VN478_00600"
     CDS             3015..>3216
                     /locus_tag="VN478_00600"
                     /inference="COORDINATES: protein motif:HMM:NF014165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21208.1"
                     /translation="MTHGGERNTMDIREFYDYCREIANYTSAAALVEWDMRTKMPPKA
                     AADRSVVAGKLARTAFEMSTSPR"
BASE COUNT          672 a          945 c         1001 g          598 t
ORIGIN      
        1 ggccagcgct catcggactt gacgagagca ggcggctggg tcacgtagct gcgacgccat
       61 aacttgacct gggcatcgcc gtacttctcg gccgtctcgg ccttgttgag tccctggagg
      121 gctccatagt ggcgctcatt gaggcgccag ctgcggacct gcgggatcca catcaggtcc
      181 atgtcgtcga ggacgatcca cagcgtccgg atggcccgct tgaggacaga agtgtatgcc
      241 aggtcgaagg tgaagccgcc ttccttcagg agtttgccgg cgtcatgcgc ttcggcaact
      301 cccttgtcgg agaggtcgac gtcagtccag ccggtgaacc ggttttcctt gttccatgtg
      361 ctttcgccat gcctgagcag tactacttcg tacatgtgtc tcctccttgg aagatcaatt
      421 cctctccagc atagccgatg cactgtcgac atcgtgaaac gtgtgtggag gcatgggcgc
      481 gatcatcggt cttcaactga tcgctcaaag cgtcctcgcg caagcacaag cgggatgagt
      541 atgacctgca cggcgatgcc gggaagcccc gtgacgatgg caccaagcac ataggcagcg
      601 ggctcagcct tcaaccctat ggatggcccc agcaaagcga ctgcgaggcc aagcgcaacc
      661 cttcctgctg ccatcgcgag aaggagggac cacagcgccg ccagcctcca gcgccgacgg
      721 gccagtcctg ccaccaggcc ataggcagct agttcgacga ccatgggagg caagatcgcg
      781 acagccggca tacctgtcat gagcgtgctg agcaaaggcg tcgcgatacc gaggaaggcc
      841 cctgccaccg ggcccagcag gagtcctccc agcaggacgg catagtgcat cggcagcaag
      901 gtcttgccgc caggggctcc gaacagagca tgaaacacga tgggcagcag caggccgatg
      961 gccagcagca ggccgagcgt cgtcagggtc cggacagaga agcgtcgagt catcagtacc
     1021 tcatcatcca gtggataggg tgcgactcac tataggaacg gcattcctgc tgtcaacctg
     1081 cgcccgcgcg aaatactgca ccaggacgcg ttgaccagtc cgtgccgtga ggctgagcga
     1141 aaggcccgac cacacaatgg tcgggcccgc cgagataatc tggagtagtc tagcgccgcg
     1201 tgatgtcgtc ctctggccct tcctgcttga caggaggctg cggaggtgcg gacatggcct
     1261 gcggaggtgc cggcggctgg aagatgccga tgtgtccgtc agtccccgac gtcaggtaga
     1321 tggcaacacc cgctgcgatg agaagcaccg gcacgataat gcgccccgca gagccaagat
     1381 ggaaaccgcc tatcccgaga acgacctcga agaagatgcc aaacccgatg aagagcatga
     1441 cagcaacgcg tatggtcttc tcggcggcgt gcaccgctgc cgcattgccc gtctgcttgc
     1501 cccacaggta cttgcccagg ccgatactgc ccgggaacag gagagcccag gcatatgccc
     1561 agctctgcca ttggcccgtg atgttctgga tgaagaggat gatgcctgtc acggcgacga
     1621 tgctgccgaa cgtgaccagg ccctcgccct tgcggcgcga ttcgtggaag ccgagcgcga
     1681 tgatcagtcc acccggtacg atgatccaga agggccacag ccagtcccca agattgatat
     1741 gaatgaacct ggcaatcagg atccaggcac ccaccgccag aagcaagagt ccaagcgctc
     1801 ttgtgttacg ctcgttctgt ccattgttgt tcatctcgta ccccctggtg ctggctttgg
     1861 agacgttcac actcagtccc cagacggagc gtgggcccgt gtatgcaccg ttcaccatct
     1921 atacggtgag tggtccggtc aggttacaga cgagtcctcg actcctgaat ggccatggcg
     1981 gactcaaggt catgtgccca ccgttgcgcc cgtatcgaaa tgctgctcgg tcccgccgca
     2041 acagggtcaa gaacgctgag ggttgctgcc atgaccgtcg atgtgctcag catgcgcgca
     2101 acgtgcttga acgccgtccc gggcgaattg cgatagctgc agaacagcgc gacgggcttg
     2161 cgggcgagag gagccgtccg aatgtagctg cggagcgcag gactgaacgt gccgagcaac
     2221 accgggctgc ccagaatgat gaggtcatag gtcgaggcat catgttccgg agggagcaga
     2281 agaggcctct gggacagcag aagaacgcgc aacgtccaga gatagtgtcg cgtccccatc
     2341 ccagacggct cccccgccgg ccgcagctcc tccagatcgg cctgcgccgc tttggcgacc
     2401 tgttcggcta tggtccgggt gtggcctgcc gaggagtagt agacgaccag gaccttgagc
     2461 tgtcttcctg caacctgcat gggcttcctc ctggcggaac atcctctgta ggaatagagt
     2521 atatacggct tctcccggct tgctacagcg agccggcggc ccgtacagac tgcagacaaa
     2581 actggatctg ttcgcttgac cccaagggta ttgacgtgtt ttttggggaa ttgtatactt
     2641 attcatggca agataggaca tgccagaatt gtgtatggag gtcatcggtg cttacaggca
     2701 aggtaaagtg gttcaacgcg gagaagggct atggcttcat cactcgtgat gacggcgaga
     2761 aggacgtttt tgtccactac agtgcgatca tcggctccgg cttcaagtcg ctgaaggaag
     2821 gcgaccttgt ggagttcgag gttgtgcagg gcgacaaggg cccgcaggcg aaggacgtcc
     2881 agaccaaggt ctgatcaccg gcacctgaac ccaatcgagc ccccggcagc tgccgggggc
     2941 ttttcgtttt cggtcccaat agtgcggata ccgattgaca tgggcgtgca cgcccgtatg
     3001 ctgtggtgtg ttggatgaca catggcgggg agcgtaacac gatggacatc agggagttct
     3061 acgactactg cagggagatc gcaaactaca ccagcgcagc agcacttgtc gagtgggaca
     3121 tgaggaccaa gatgcccccc aaggcggcag cggatcgttc ggtggttgcg ggcaagcttg
     3181 cgaggactgc gttcgagatg tcgacatctc cgcgca
//
LOCUS       DATMZD010000027         2050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_111554,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000027 DATMZD010000000
VERSION     DATMZD010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111554"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="VN478_00605"
     CDS             complement(<1..651)
                     /locus_tag="VN478_00605"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HWQ21209.1"
                     /translation="MRRQVSFLASRARLWLQNPLPFAVLVGAALLYVGALVSGLAEDV
                     LDSDSIVLADGRLVHWLAAHRTPFGTGFFEAVSFLGNWQVAVLVLVVSIVLLWNRRRR
                     SALAMMVAAAGSQIVVSLGKAGFGRPRPPAVLAVVTPLDASFPSGHSSISVALYALGF
                     YLLCRSTRSPRLKRLWLGLAFLLPLLIGFSRLYLGAHYLSDVLAGWTIGLWWSILVV"
     gene            768..1487
                     /locus_tag="VN478_00610"
     CDS             768..1487
                     /locus_tag="VN478_00610"
                     /inference="COORDINATES: protein motif:HMM:NF025559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HWQ21210.1"
                     /translation="MEDTNTRETFTAEHHALLFAWIAREVIARVGESAAGPVLRETVR
                     LYGEQRGRRMALRAQQDGQPLSMASYLSYREWQAPAGAMQQTGVPWRADLRTQVRRCC
                     WATTWQQEGLTEYGKYYCQEIDEALVRGFNPDLVVEVKGTRTNGSRACQLIYRGAFEG
                     TLVHDEMQRDQEKRILPWSYHTAHLYSAMSTVLQRELGPVGTAAAQAALGAFAARFGR
                     AMADVLTADAVADFHVLPEER"
     gene            1484..>2050
                     /locus_tag="VN478_00615"
     CDS             1484..>2050
                     /locus_tag="VN478_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014321765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HWQ21211.1"
                     /translation="MTWSVRCSRCGRVYDDDATRFRCDCGGVLDLLFEPQFDPAAVAG
                     RPHDLWRYREALPVSGDIVTMGEGCTPLLPVPFRTATLLVKQEQLFPTGSYKDRGAAL
                     LISRLRELGVERVVEDSSGNAGCAVAAYAARAGIACDIYVPADTSPAKTAQIEAYGAT
                     LHRIAGTREDTASAAMEAAATAVYASHVY"
BASE COUNT          411 a          622 c          705 g          312 t
ORIGIN      
        1 cacgacgaga atggaccacc acagcccgat cgtccatccc gcgaggacgt ccgacaggta
       61 gtgcgcgccg aggtacaggc gactgaaccc gatcagcagg gggagtagaa atgccaggcc
      121 gagccagagc ctcttgagcc gcggagaccg cgtcgaacgg cacagcagat agaagcccag
      181 cgcatagaga gctaccgaga tgctggagtg cccggacggg aagctcgcat cgagcggcgt
      241 gacgaccgca aggactgcgg gggggcgagg cctgccgaag ccggccttgc caagagagac
      301 gacgatctgt gagcctgcag ctgcgaccat cattgccagc gccgatcgac gcctgcggtt
      361 ccacaggagc acgatggaaa cgaccagcac gaggacggcc acctgccagt tgccgaggaa
      421 gctgacagcc tcgaaaaagc ctgtgccgaa tggtgttcgg tgggccgcca gccagtgcac
      481 gagtcgtccg tcggccagca cgatgctgtc gctgtccagc acgtcctcgg ccagacctga
      541 gacgagagcc ccgacataca ggagagctgc tccgaccagc acagcgaagg gcaaggggtt
      601 ttgcagccag agacgcgctc gactggccag gaacgatacc tgtcttctca tggagacatg
      661 atagcagaac tgaggcgaaa agaagcaatt ccggacttgc ctgaagagac cgtcccgata
      721 gacttcagag acgggaacct atggagcggg tattccagga ggcacgattg gaggacacga
      781 acaccagaga gactttcacc gccgaacatc atgcgctgct gttcgcgtgg atcgcccggg
      841 aagtgatcgc gcgggtgggc gagagcgcgg cagggcccgt cctgcgggag actgttcggc
      901 tctacggcga acagcggggc cgtcgcatgg cactgcgggc acagcaggac gggcagcccc
      961 tttctatggc gagctacctg tcgtatcgcg agtggcaggc ccctgccggc gcaatgcagc
     1021 agaccggcgt gccatggcgc gcggacctcc ggacgcaggt gcgtcgctgc tgctgggcta
     1081 cgacatggca gcaggaggga ctcacggagt acggcaagta ctactgccag gagatcgacg
     1141 aggccctcgt gcgcgggttc aaccccgacc tggtcgtcga ggtgaaggga accaggacca
     1201 acggaagccg ggcgtgtcag ctcatctacc gtggcgcgtt cgaggggacg ctggtccatg
     1261 acgagatgca gcgggatcag gagaagcgga tcctgccgtg gagctaccac acggcgcacc
     1321 tctacagcgc gatgagcacg gtgctgcagc gcgagctggg tcctgtcgga acggctgccg
     1381 cacaggcggc actgggtgcc tttgctgcgc gattcggccg tgcgatggcg gacgttctca
     1441 ctgcagacgc ggtcgccgat ttccacgtgc ttccggagga gagatgacct ggtcggtgcg
     1501 ctgctcacgc tgtggaagag tctacgacga tgatgcgaca aggttccgct gcgactgtgg
     1561 cggtgtcctc gatctcctgt ttgagccgca gttcgatccc gcggcagtgg ccggtcgacc
     1621 gcatgatctc tggcggtacc gcgaagcgct gccggtcagc ggcgacatcg tcacgatggg
     1681 cgagggatgt actcctctgc tgcccgtgcc gttccgcact gcaactctcc tggtgaagca
     1741 ggagcagctc ttcccgacgg gatcgtacaa ggaccgcggg gcggcactcc tcatcagccg
     1801 tctgcgggag cttggggtcg aacgtgtcgt cgaagactcc tcgggcaacg ccggatgtgc
     1861 ggtggcggcc tatgcggctc gcgcgggtat cgcctgcgac atctatgtcc cagcggatac
     1921 atcgcctgcg aagacagcgc agatcgaagc gtacggggca acgctccacc gcattgcggg
     1981 cacacgcgaa gacactgcga gcgccgcaat ggaggcagcg gccactgcgg tctacgccag
     2041 tcacgtgtac
//
LOCUS       DATMZD010000028         2643 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_40892,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000028 DATMZD010000000
VERSION     DATMZD010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40892"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VN478_00620"
     CDS             <1..181
                     /locus_tag="VN478_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007103291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HWQ21212.1"
                     /translation="PIRIGSQVWIGAGAIINPGVTIGDRAVIASGAVVTRDVPADTLV
                     AGVPAVPKKHIDNEA"
     gene            327..1019
                     /locus_tag="VN478_00625"
     CDS             327..1019
                     /locus_tag="VN478_00625"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013655380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HWQ21213.1"
                     /translation="MKELDDPVIIGFPLRGEWTAPTTPAKRIPSHGTDQLGERYAFDF
                     VRVDWARTGRPFYRASLLQYLFSGVPLNKCYGWGQGIYAPCDGEIIKAEDGYKERQRV
                     HWFSDSSVALRNAYTFNPEKSDIRTVAGNYIVMKCADHVYAAFAHLQAGSITVSAGQV
                     VRRGDVLGRVGHSGNSTAPHLHFQLMDSSDWRSAKGIPCAFEQYELSQDGEWETVYRG
                     VPSDKDRIRFEP"
     gene            1078..1725
                     /locus_tag="VN478_00630"
     CDS             1078..1725
                     /locus_tag="VN478_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21214.1"
                     /translation="MKGLLLYGLKCQPWIWDPMRGDLADCDIEYVEYPEEVTRTCMSV
                     SDLTAWVGEQYLSRGQAYDFVLGHSMGGLIVLQLSALDGARFTKTIFVESFLKPSEPF
                     YHNLMMDANMAAMGNKVLAMFSEEDAKYTPELKASLKEDFDYTGSLDHIANEVFGIYG
                     DRGNRDRSLLLRNLDLDDQQLGKLDISFIRESCHLPMLESPHELARRILAILQQS"
     gene            complement(1722..2246)
                     /locus_tag="VN478_00635"
     CDS             complement(1722..2246)
                     /locus_tag="VN478_00635"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HWQ21215.1"
                     /translation="MYTGKIRIKETDQSDLPNIMQLWNNGQVMFYVGFPSGLGVTMER
                     LQQWLEGINQDIYSRHYSIYAQDIGYCGETFYRVDRGHDLATLDIKLLPRAQGKGIAA
                     YAFSHVIDQVFHNNLATKAYVDPHPKNKKAWNLYARLGFVSKPRPKFLPKGPTYLEVT
                     PDTFQSAYPGSAAV"
     gene            complement(2316..>2643)
                     /locus_tag="VN478_00640"
     CDS             complement(2316..>2643)
                     /locus_tag="VN478_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016297513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HWQ21216.1"
                     /translation="IRAVQSHGYELCCDVKPEHVMEKVLYTIDELTGLINACAIMRPS
                     KSVLDLELKSVMKKYKDHRFAAGVNREVIEKGAAMLGMDLDKVITETIMGMREVAEAI
                     GLKGGL"
BASE COUNT          563 a          700 c          783 g          597 t
ORIGIN      
        1 gcctatacga atcggcagcc aggtatggat cggcgccggc gcgatcatca accccggcgt
       61 cacgatcggt gaccgggcgg tcatcgcctc aggcgccgta gttacgaggg atgtcccggc
      121 cgatactctt gtggccggag tcccggctgt ccccaagaag catatcgaca acgaagcctg
      181 agccacctct agccgttgct tgctgataat agaggatggg cataggaaga cgtctagccg
      241 caggattgat tctcacttta cttgcgatca ctatactggc tgacatggtg ggtaacgctt
      301 cgcactcagg aacggggagt gatgcaatga aggaacttga cgatccggtg atcatcggat
      361 ttccattaag gggagagtgg acggcgccca ccactccggc gaagagaatt ccaagccacg
      421 gaacagacca attgggagag cgctatgctt ttgattttgt acgggttgac tgggcaagaa
      481 cggggagacc tttttaccgg gcaagcctgc ttcagtatct cttctcgggc gttcctctga
      541 acaagtgtta tggctgggga caaggaatat acgcgccatg tgacggggag atcatcaagg
      601 ctgaagacgg ctacaaggaa aggcagagag tccattggtt ttccgactcg tcggtcgccc
      661 taaggaatgc ctatacgttc aaccctgaga agagcgatat tcggaccgtt gccggcaact
      721 acattgtcat gaagtgtgct gaccacgtat acgccgcatt cgcccatctg caagcggggt
      781 ccattacggt atcggcaggt caggttgtga gaagagggga tgtattggga agagtagggc
      841 attcaggcaa ttcaacagct ccacatcttc atttccagct catggacagc agtgattggc
      901 gttcagcaaa gggcattccc tgtgctttcg agcagtacga attatctcag gacggtgaat
      961 gggagacagt atatagaggt gttccttcag acaaggacag aatcagattt gaaccttgat
     1021 cgacgcagca tcagcacctg cacgcgagtt gtggtatcgc gtcgtcggag gaaatgagtg
     1081 aaaggcctgc tattgtatgg gttgaaatgc cagccgtgga tctgggatcc gatgagaggc
     1141 gatctggctg attgcgacat tgagtatgtg gagtatccgg aagaagtgac ccggacgtgc
     1201 atgtctgtca gcgacctgac agcatgggtg ggcgagcagt atctgtcccg tggtcaggca
     1261 tatgacttcg tcctgggaca cagcatgggt ggattgatcg tcctgcagtt gtcggcgctg
     1321 gacggcgccc gcttcacaaa gacgatcttc gtggagtcgt tcctgaaacc ctcagaaccc
     1381 ttctaccaca atctgatgat ggacgccaac atggcggcga tgggcaacaa ggtgctggcg
     1441 atgttcagcg aagaggatgc caagtacacg cctgagttga aggccagcct gaaagaggac
     1501 ttcgactaca cgggatccct ggatcacatt gccaatgagg ttttcggcat ctatggcgac
     1561 cgcgggaatc gagaccgttc cctcctgttg cgcaacctgg atctcgacga ccagcagctc
     1621 ggcaaactag acatcagctt catacgcgaa tcctgccatc ttcccatgct ggagagcccg
     1681 catgagctgg cgcggcggat ccttgcgatc ctgcagcaga gctagacggc tgcagaaccc
     1741 gggtaggcgc tctggaacgt gtccggagtg acctccaggt aggttggtcc ctttgggagg
     1801 aacttgggtc gtggcttgct gacgaacccc agcctcgcat agaggttcca cgccttcttg
     1861 ttcttcgggt gagggtcgac gtatgccttc gtggccaggt tgttgtggaa tacctggtcg
     1921 atgacgtggc tgaacgcata ggcggcgatg cctttgcctt gcgctctcgg cagaagcttg
     1981 atgtccagtg ttgccaggtc atgtccacgg tcgacgcggt agaacgtctc gccacaatac
     2041 ccgatgtcct gagcatagat gctgtaatgc ctgctgtaga tgtcctggtt gatcccttcc
     2101 aaccactgct ggaggcgctc catcgttacc cccagaccgc tgggaaagcc gacgtagaac
     2161 atcacctggc cgttgttcca cagctgcatg atgttcggca ggtctgattg atcagtctct
     2221 ttgatcctga ttttgccggt atacaaggag cgccccctga gaactgttgt cgtcaccccc
     2281 gcgaaggcgg gggtccatct gttgcgatgg ttggtctaca gtcctccctt gaggccgatg
     2341 gcttcggcga cttcgcgcat gcccatgatg gtctcggtga tgaccttgtc caggtccata
     2401 cccagcattg cggcgccctt ctcgatgact tcgcggttga cgccggcagc gaatctgtgg
     2461 tccttgtact tcttcatgac ggacttcagc tccaggtcca ggacactctt cgacggccgc
     2521 atgatcgcgc acgcgttgat gaggccagtc agctcgtcga tggtatagag gaccttctcc
     2581 atgacatgct ccggcttcac gtcgcagcac agctcatacc catggctctg gaccgcacgg
     2641 ata
//
LOCUS       DATMZD010000029         2165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_71372,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000029 DATMZD010000000
VERSION     DATMZD010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71372"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1103
                     /gene="glgP"
                     /locus_tag="VN478_00645"
     CDS             <1..1103
                     /gene="glgP"
                     /locus_tag="VN478_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HWQ21217.1"
                     /translation="DSHAVFAGVELIRQQMTTGVPFTDALLSVKRHIVFTTHTPVSAG
                     NEVHTHRILQYMNAYDGLSSEQIVQLGGNPFSMTAAGLRLSRKANAVSQLHGNTARQM
                     WKLIPGGSDILSITNGVHNGTWQDESIRQAHASGGDLWAAHSALKHVMIDDIQRRNGV
                     RLDENVLTIGFARRATPYKRSDLIFSNREIIGDLLRNHKVQIVLSGKAHPNDMEGKAT
                     ITNLVHIAKEYPESVVFVQGYDMAIGKMLTRGCDVWLNNPVRPMEASGTSGMKAAMNG
                     VLNLSTLDGWWPEACKHGVNGWQFGGGYEGNDCNSVDVVSLYHVLSAEVIPTYEERRD
                     AWIDMMHASIEMSQWEFSAARMLQDYYEKMYV"
     gene            complement(1173..1844)
                     /locus_tag="VN478_00650"
     CDS             complement(1173..1844)
                     /locus_tag="VN478_00650"
                     /inference="COORDINATES: protein motif:HMM:NF017927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HWQ21218.1"
                     /translation="MILQSIRYGWNKARLWVWAFLPALALQWVLDGAVTWREEANGTR
                     SLQLWWHNMGPQAQGIAIMAVVLSIVAYIVSMWVRMGIVHGSVQAARGENVTAGDFFV
                     GWDNVGQFILATLLYGLIVGVGMILLIVPGFIWGLRYCMYGYYVVTKGAGPVEALQMS
                     AAATDGHKGELFGLCLASMGVVILGALCLGVGLLWALPTVEMAWSAVFLELSGQQTVP
                     VPQPA"
BASE COUNT          470 a          704 c          614 g          377 t
ORIGIN      
        1 acgacagcca cgctgtcttt gccggcgtcg aactcatccg ccagcagatg acgacgggcg
       61 tgcccttcac ggatgcgctc ctgtctgtga agcgacatat cgtcttcacg acgcacacac
      121 cagtatctgc cggcaacgag gtgcacacgc accgcatcct gcagtacatg aacgcgtatg
      181 acggactctc cagtgaacag atcgtccaat tggggggcaa cccgttcagc atgaccgccg
      241 cgggcctgcg cctgtccaga aaggcaaacg ccgtgtcaca actgcacggg aacacggctc
      301 gccagatgtg gaagctcatc cctggaggct cggacatcct gtcgatcacc aatggcgtcc
      361 acaacggcac ctggcaggac gagagcatcc ggcaggctca cgcgtccggc ggcgacctgt
      421 gggctgccca cagcgcgctg aagcatgtga tgatcgacga catccagcgg cgcaatggcg
      481 tgcggctcga cgagaacgtc ctgaccatcg ggtttgcccg tcgcgctacg ccctacaagc
      541 gcagcgacct catcttctca aaccgcgaga tcatcggcga cctcctgcgc aaccacaagg
      601 tccagatcgt gctgtccggc aaggcacatc ccaacgacat ggagggaaag gcgaccatca
      661 cgaacctcgt ccatatcgcc aaggagtatc ctgagagtgt cgtgttcgtg cagggctacg
      721 acatggccat cggcaagatg ctgacacgcg ggtgcgacgt ctggctgaac aacccggtcc
      781 gaccgatgga agcgagtggc acttcaggga tgaaggcggc gatgaacggc gtcctcaacc
      841 tgagcaccct cgacggctgg tggcccgaag cctgcaagca cggcgtcaac ggctggcagt
      901 ttggtggagg atacgagggc aacgactgca atagcgtcga cgtcgtttcg ctctatcacg
      961 tcctctcggc cgaggtcatc ccgacctacg aagaacggcg ggacgcatgg atcgacatga
     1021 tgcatgccag catcgagatg tcgcagtggg agttctcggc ggcgcgcatg ctgcaggact
     1081 actacgagaa gatgtacgtc tgacgagact gttcacaagg atcacgtcat gtagaacaag
     1141 gaggggccct acggcccctc cttgtgtttc cgctatgccg gctgaggcac cgggactgtc
     1201 tgctgtccgg agagctccag gaagacggca ctccaggcca tttcgacggt aggaagggcc
     1261 cacagaagcc caacgccaag acacagggcg cccaggatga cgacacccat cgaggcaagg
     1321 cagagaccaa acagttcgcc cttgtgcccg tcggtcgctg ctgcgctcat ctggagcgct
     1381 tcgacaggac ctgccccctt cgtcacgacg tagtagccgt acatgcagta gcgaagtccc
     1441 cagatgaagc ctggaacgat cagcaggatc atgccgacgc cgacgatgag accatacagc
     1501 agcgtggcca gaatgaactg cccgacattg tcccacccga cgaagaagtc gcctgcagtc
     1561 acattctcgc cgcgcgcggc ctgcaccgat ccatgcacga tgcccatccg gacccacatc
     1621 gacacgatgt aggccacgat actcaggacg accgccatga tggcgatccc ctgcgcctga
     1681 ggccccatgt tgtgccacca gagttgcagg ctcctcgtgc cgttcgcttc ctcacgccac
     1741 gtcacggcgc catccagaac ccattgcagc gcgagcgccg gcaggaatgc ccacacccac
     1801 agacgtgcct tgttccaccc gtaccgtatc gattgaagta tcatcgtagc agctccttgt
     1861 cccagtcatg cgctcagttt gtacctgcgc tacaccaagg tatacagctg tccaacatcg
     1921 caggttacgt ccctcccgcc tgtccgcact ctatctgtcg ttccccccga gtatttctgt
     1981 gtgatacgag gggcgttcct ggcgaaggcg ggaacccact tccgaagtga gccacaagac
     2041 tcaggaagcg ctagtccgtg atagccctct gccaagactc ctccactcgt accatgtcct
     2101 gatcgtccgg aacagtctct tgtcctgaga gcttgaggaa gatgacgccc ccggcaatat
     2161 cgacg
//
LOCUS       DATMZD010000030         2870 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_31066,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000030 DATMZD010000000
VERSION     DATMZD010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31066"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..437)
                     /locus_tag="VN478_00655"
     CDS             complement(87..437)
                     /locus_tag="VN478_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21219.1"
                     /translation="MRMRSMSVWTRLVLVIYLAAVVAVCLYVPAHASEPILTHKPPTG
                     VAVLSGPAMASFGYRRVWDLTETYSVVMGGDLYYYRTEATVDYRLIVLELVGLTAGAG
                     VLLLVGSLFGGRDR"
     gene            complement(489..707)
                     /locus_tag="VN478_00660"
     CDS             complement(489..707)
                     /locus_tag="VN478_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21220.1"
                     /translation="MPESAGAHVDTHVHVRAGELTKEELEAKRQAEAAEEEEDERKKD
                     EEEKTPAPLWENPGQSNSPQKHRRLGKK"
     gene            complement(996..1292)
                     /locus_tag="VN478_00665"
     CDS             complement(996..1292)
                     /locus_tag="VN478_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013404092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="demethoxyubiquinone hydroxylase family protein"
                     /protein_id="HWQ21221.1"
                     /translation="MPAFANPFQANVERQMTKKELMQALRLDVAGELEAIYLYDAHVQ
                     ATDDTRAQKVLADIRDEEKVHVGELMALLRTLDPQEAELFLDGAAEVKKLLSEP"
     gene            1540..2091
                     /locus_tag="VN478_00670"
     CDS             1540..2091
                     /locus_tag="VN478_00670"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HWQ21222.1"
                     /translation="MAKNIGVLVGSLRKESYSKKIARVLAGLFPEDCNVSFVEIGDLE
                     LYNQDYDDEGRVPESYTGFRSTMSNVEAVLFVTPEYNRSVPAVLKNALDVGSRPKGKS
                     IWDGKPAAIVSVSPGAIGAFGANHHLRQSLVFVNMPTVQQPEAYIGHVNSILNESGEV
                     TDAGTLKFLRSVVDALVALADRQ"
     gene            complement(2569..>2870)
                     /locus_tag="VN478_00675"
     CDS             complement(2569..>2870)
                     /locus_tag="VN478_00675"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HWQ21223.1"
                     /translation="TLKVLGYTSWEISRMVLVSTAVPVVLGYAVAVPFLVRYLHILLA
                     LNQQNLDFALPVSVSPWGVVIGFVVIGIVWGGSTALAMRSLFHVPLADSLKAARE"
BASE COUNT          621 a          867 c          769 g          613 t
ORIGIN      
        1 gacgtgcgaa aggaccatcc catggtgcac cggcagacca ggaagtcaag atctcgcgac
       61 gtgtgcctgc atgaggcggt gctgccttac ctgtccctgc ctccaaagag cgacccgaca
      121 agcaacagga cccccgcgcc agcagtcaga ccgaccagct ccaagacaat cagtcggtag
      181 tcgaccgttg cctcggtcct gtagtagtac aggtctcctc ccatgactac gctgtaggtc
      241 tcagtcagat cccagacgcg cctgtagcca aatgaggcca tagcaggacc tgaaagcacc
      301 gcaacacccg ttggcggctt atgggtcagt attggttcgg acgcgtgcgc tggcacgtag
      361 agacacactg caacaacggc agccaggtag atcaccagta caagtcgagt ccatacactc
      421 attgatctca ttctcaatct cgctcctctg cctaggcgcc cctatgcatt gcggtcatcg
      481 tcgtagaact acttcttccc aagtctgcga tgtttctggg gagagttcga ctgtcccggg
      541 ttctcccata gaggagcagg cgtcttctct tcctcgtcct tcttcctctc gtcttcctct
      601 tcttctgctg cctctgcttg tctcttggct tcaagctctt ctttggtcaa ttcgccagca
      661 cgaacatgta cgtgagtatc tacgtgggct cctgcggatt cgggcatatg tcgcctcctt
      721 ttgcgtcgcc agtcttccga cagtcttcag agtgcaactc actacaagac cgatcacggc
      781 agtcagagat gagactactt caggacgtga cccctgccgc gcgcttgtcg aaacagggac
      841 aacatcggaa gcgtcctgcc catcttcttc atgaccagct cgtgctcgca gttcggcgcg
      901 gacggttcag gaagctgctg tcctcgactc ccacagtctg tcccgtacgg ttccatcgcc
      961 gatcccgccc gcgaggtcct gggagattcc acgtgctacg gttcgctcag cagcttcttg
     1021 acttctgcag cgccgtcgag gaagagttcg gcttcctgcg gatccagcgt cctcaggagc
     1081 gccatcaact ctcccacgtg caccttctct tcgtcacgga tatcggcaag taccttctgt
     1141 gccctcgtgt cgtccgtggc ctggacgtgt gcgtcataga gatagatggc ttcaagttcc
     1201 cctgcgacat ccagacgcag ggcctgcatc aactccttct tcgtcatctg tcgttcgaca
     1261 ttagcctgaa aaggattcgc aaaagccggc atctcatcga cctccgtctg tcagagtcca
     1321 ctgcacttct atcgtcaagt atagggtttc tccatgaaga tacacgagaa gagagttgta
     1381 tcgccgcgat cctcggtgac atccgtccgt ggtatcaaga acagaacacc acaaagtggt
     1441 ctttcaaagg acacctttgc tgctagggta agcttgattg taggattctc ccccagattg
     1501 ttggtctgcc gtcgcaaagt agatgtagag gagcccaaca tggcaaagaa cataggtgtg
     1561 ctggtcggaa gtctgcgcaa ggagtcctat agtaagaaga ttgccagagt cctggcgggc
     1621 ctgtttccgg aggactgcaa tgtctccttc gtcgaaatcg gtgatcttga gctgtacaac
     1681 caggactacg acgatgaagg aagagtccct gagtcctaca caggattccg cagcacaatg
     1741 tcaaatgtag aggccgtact atttgtgacg cctgagtaca atcgttcggt cccggcggtc
     1801 ttgaagaacg cgctcgacgt cggatcccgc cccaagggca agagcatctg ggatgggaaa
     1861 cctgcagcga ttgtcagcgt ctctcctggc gcaatcgggg cgttcggagc aaaccatcac
     1921 ttgcgccaat cactggtctt tgtgaacatg ccgacggtac agcagcctga ggcatacatt
     1981 ggacacgtca acagtatcct taacgagagt ggagaggtca ccgacgcagg aaccttgaag
     2041 ttcctccggt ctgtcgttga cgctcttgtt gctctggccg acagacaata gggggccagg
     2101 cgcaggggca aggcatagct gagcaagccc ggttgcacct gttcccagtg cctcatctct
     2161 accacggctg catcattgat gcgccttggc gtcgtccccc aaaccctggc gactcctgcg
     2221 gacgtatact gtactccccc gctgacacca cagcagccgc aacgcaggtc cgtgacatca
     2281 accggccaca aacactatcc tcgactggag gttcgcagtc tctctcttgt gagccgtacg
     2341 cctgtcggag cacatttcgc agtctccgcc gcccgcgagg aagcccgatg tgaagcggaa
     2401 gaagggaggc agcgcgccac agcggagacc taatcccatg actgtgagga gagcaggtaa
     2461 tgttcacctg ccccttagct cccgcaatgt gcctgcttgc gccaattctg cagtggtaca
     2521 tactccaaac gtcagggaac aacgacgagc gacgagtgtg cccagatctc actcgcgtgc
     2581 agccttcagg ctgtcagcga gggggacatg gaacagtgac ctcatcgcca gagctgttga
     2641 ccctccccag actatcccta tcaccacgaa gccgatcacg acgccccaag gtgagacaga
     2701 caccggcagc gcgaagtcca ggttctgctg gttgagcgca agcaggatgt ggaggtagcg
     2761 taccagaaag gggacagcga cggcgtagcc aagcacgaca gggacagccg tcgaaacgag
     2821 caccatccgt gagatctccc aactcgtgta gccgagaacc ttgagggtcg
//
LOCUS       DATMZD010000031         3195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_141607,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000031 DATMZD010000000
VERSION     DATMZD010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141607"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..922)
                     /locus_tag="VN478_00680"
     CDS             complement(<1..922)
                     /locus_tag="VN478_00680"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWQ21224.1"
                     /translation="MRRLLAIILVCALIAGVGVGCKAKVGVANLGGIFPMTGGSATFG
                     TSSKNGVTMAVEEFNTAGGATVDGVRTTINYVNEDDTGSPEVGASAAHKLIDQDKVIG
                     IIGAVMSKVSLAVAPICQDAGVPQISPTSTNATVTLVGDYIFRACFIDPFQGTVMANY
                     VWNTAKLKTAAVLYDNGNDYNKGLAENFKAGFEKNGGKIVAYEAFTDEDKTVDYKAQL
                     TKIKAANPEFLYLPNYYASAATILKQARGMGLNIPAGGGDGWDSPDLVKIGGAAVEGG
                     VFSNHFSKDDPAPKVQAFVAAYTAKYGEAPD"
     gene            complement(1611..2843)
                     /locus_tag="VN478_00685"
     CDS             complement(1611..2843)
                     /locus_tag="VN478_00685"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWQ21225.1"
                     /translation="MSAFDLTIKDLTQILRDRKSFMFILIMPIVFTLLFGFAFGGYGS
                     PGDTRLPVVVAGDAAEPAHAAFSDLVASSTVVRLVEPASAEDHALRASVRDGDVTAVV
                     FIPSGYLRSCYDGSDPVLPCVVDVASTNGTTARNELANLSTRLASAVRTARATAAIYA
                     DVAPHPASGKPATFTFDQFARAFETSASAWKAQPPTVAVTVSSKLTEQQRNAGGFAHT
                     SPSMMLQFSIAGLMSAAGIVVEERKSRTLARLMTTRLSRSGILLGHYLAMFIQTFVQL
                     SMLILFAALVLRVQYMHAPFATLLVTVCASAFVAAIGLLIGVASRSEEQAIAFSLILM
                     FALAGLGGAWVPLQFTGAAFQRVARATPGALIVDAYENVVQRGLGTSSVLMPCALLLA
                     CAAGLAVLAVLRFHRQSA"
     gene            complement(2852..>3195)
                     /locus_tag="VN478_00690"
     CDS             complement(2852..>3195)
                     /locus_tag="VN478_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21226.1"
                     /translation="IALIPTAMCASAFGIFLMSFLKTTRQAGIMFGGVVTATGMLALA
                     SVFTMSVPSAARFGQMVALFVPQGWSLLAFRQAMDGAPMSRLFTSAAVVLAWTAFMFI
                     VGRTRMARRFA"
BASE COUNT          647 a          943 c         1029 g          576 t
ORIGIN      
        1 cgtcgggcgc ttcgccgtac ttggctgtgt aggctgcaac gaaagcctgg accttgggag
       61 cgggatcatc cttggagaag tggttggaga agacgccacc ctcgactgcc gcaccaccga
      121 tcttgacgag gtcgggtgag tcccagccat caccgccgcc ggcggggatg ttcagaccca
      181 taccgcgtgc ctgcttgagg atggttgcgg ctgacgcgta gtagtttggc agatacagga
      241 actcagggtt ggctgccttg atcttggtca gctgggcctt gtagtccacc gtcttgtctt
      301 catcagtgaa cgcttcgtat gcgacgatct tgccgccgtt cttctcgaat cccgccttga
      361 agttctccgc gagacccttg ttgtagtcat tgccgttgtc gtacagcact gcggctgtct
      421 tcagcttcgc cgtattccag acgtagttgg ccatgacggt tccctggaac gggtcgatga
      481 agcaggcgcg gaagatgtag tcgccaacca gcgtgactgt cgcatttgtg gacgtcgggg
      541 agatctgcgg tacaccggca tcctggcaga taggtgcgac agccagcgag actttgctca
      601 tgacggcgcc gatgatgccg atgaccttgt cctggtcgat gagcttgtga gctgcgctgg
      661 ctccgacttc aggcgaaccg gtgtcatcct cgttgacgta gttgatcgtc gtcctgacgc
      721 cgtcgacggt tgctccaccg gctgtattga actcttcgac ggccatggtg acgccgttct
      781 tgctggatgt gccaaaggtg gccgaaccgc cggtcatcgg gaagatacca ccaaggttgg
      841 caacaccgac cttcgccttg catccaacgc caacgccggc aatcagtgca cagacaagaa
      901 taatggcaag cagtctcctc atgtattctc ctctcatgat gcgaatgtgt ggtaccagtt
      961 aatggagcag tagagcatcg tcaggcttgc aggcccgttg acaccccctt cccaagatag
     1021 caaaaagacc tcacggcgtg ctttccgcaa ggtcttccat gtgctgcgca agctgagggc
     1081 ttgggtccct gctcactgca gactccgggc acagtgccgc ccggcgggtc cgagtcacag
     1141 ccgtccggta ccaggcgtga gcatgaaccg aatgggtgtg gtgagtcgag gtatgagaca
     1201 aagaggggga accatctggt gtccatagtg cattcttgca gagtcggcag aaaagtcaat
     1261 agatgacgca tgtgttccat cgtagagcta ctggagcgca tcaaccggca cggcccggca
     1321 tactgcaagg tgcgcgatcg caagcggtcc acacagtggt gattcgacgt gcagggccgg
     1381 catgagcgcg caaacatcgt cggcgcgcag aatcgcagcg gaacggacct gtgcattcgc
     1441 cgtttcagcg cctcacatca catcggacgt ccctgttccg caatagatta cgcgtcgctc
     1501 catgtattcg acctcctgat gcacactatt gcggagtggt gggaattgtc aaggcggact
     1561 cggccgcacg tccatccgac gcccgctgcc gcgccgaaac gaggcgcacg tcaggcggac
     1621 tgcctgtgga agcggagcac cgccaggacg gcaaggcctg ccgcgcaggc tagcagcagt
     1681 gcacatggca tgagcacgga cgacgtgccc aggcctcgct ggacaacgtt ctcatacgcg
     1741 tccacgatca acgctcccgg ggtcgcccgc gccactcgct ggaatgccgc tccggtgaac
     1801 tggaggggca cccacgcgcc tccaaggccg gccagggcga acatgaggat cagcgagaac
     1861 gctatggcct gctcctcaga gcgtgatgcg acgccgatga gaagaccgat cgccgcgacg
     1921 aatgccgagg cacagactgt cacaagcagc gtcgcgaaag gagcatgcat gtactggaca
     1981 cggaggacca gggcggcgaa gaggatgagc atcgacagct gtacaaaggt ctggatgaac
     2041 atggccaggt agtgacccag caggatgccg gaacgagaca acctggtcgt catgaggcgc
     2101 gcaagagtcc tggacttccg ttcttcgacg acgatcccgg cggcactcat cagtccagcg
     2161 atggagaact gcagcatcat gctcggggac gtgtgcgcaa agccgcccgc gttgcgctgt
     2221 tgctccgtga gtttgctcga taccgtcacc gcgacggtcg gcggctgggc tttccaggcg
     2281 gacgccgagg tctcgaacgc tcgtgcaaac tggtcgaacg tgaatgtcgc gggcttgccg
     2341 gacgctggat gcggcgccac gtcagcgtag attgcggccg tcgcacgcgc agtgcgtacg
     2401 gcactggcaa ggcgtgtcga caggtttgcc agctcgtttc tggcagtcgt tccattggta
     2461 ctcgcgacgt cgacgacaca cgggagcacg ggatctgacc catcgtagca gctccgcagg
     2521 tatccagagg ggatgaagac gaccgcagtg acgtctccgt cgcgcacaga ggcgcgaagg
     2581 gcatggtcct ccgcacttgc gggctcgacc aggcggacga ccgtcgacga cgccacgagg
     2641 tctgagaatg ctgcgtgtgc aggttcggcg gcgtcgccgg cgacgacgac cggaagacgg
     2701 gtgtccccgg gggagccgta gccaccgaac gcgaacccga agaggagcgt gaacacgatg
     2761 ggcatgatca ggatgaacat gaaggacttg cggtcacgca ggatctgcgt gaggtccttg
     2821 atcgtcaggt cgaaggcgct catggcaggc actacgcgaa ccgccgcgcc atgcgggtgc
     2881 ggccgacgat gaacatgaac gccgtccagg cgagtacgac tgccgcgctc gtgaacaggc
     2941 ggctcatggg ggctccgtcc atggcctgcc ggaaggcaag cagtgaccat ccctgtggca
     3001 cgaacagggc gaccatctgc ccgaacctgg cagcagacgg cacgctcatc gtgaacacgc
     3061 tagcaagagc aagcatgccg gttgcggtca cgacgccccc gaacatgatg cccgcctgcc
     3121 gcgtcgtctt caggaacgac atgaggaaga tgccgaaggc agaggcgcac atcgccgtgg
     3181 gaatgagggc gatgg
//
LOCUS       DATMZD010000032         3032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_81448,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000032 DATMZD010000000
VERSION     DATMZD010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81448"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="VN478_00695"
     CDS             complement(<1..501)
                     /locus_tag="VN478_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21227.1"
                     /translation="MTRSRPYSLRVLSVILVVAGLVFTSALVVQVMSVHQQSDINEVG
                     RLEAAAVILSSAYAEALHSGGLVAPLVGGDLPSGLRLTILNTSGSAVADSEYDLASVT
                     NRIADPEVKQAAATGMGVIVSYRIESGQRERTVCVAVRDAGSLAGFAAASSPVGLTWL
                     SASRTLS"
     gene            complement(498..1172)
                     /locus_tag="VN478_00700"
     CDS             complement(498..1172)
                     /locus_tag="VN478_00700"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWQ21228.1"
                     /translation="MLEDDDDIRELICLYLKRDSMEPLPFASPVTFLSWLDAQSQPPD
                     LLILDLMMPELSGGDVIKEVRKRPSCNTMSIVVCSAKATESDVVLTLGLGADTYIRKP
                     FNPHELVAQVGAMLRRSQTSPPAFRQEALRAGPIAIDSARKTASVGGKSIDLTPAEYH
                     ILTALVEQKGLVVSREELLRATGETDAVERVVDVHITSIRRKLSYAAHLLRTSRSFGY
                     YIEDDA"
     gene            complement(1647..2846)
                     /locus_tag="VN478_00705"
     CDS             complement(1647..2846)
                     /locus_tag="VN478_00705"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HWQ21229.1"
                     /translation="MRRLLSVLLIVVLAASIFTGCKKATPVVDEIKIGLVTDVGGRGD
                     QSFNDSALRGLEIWAAKKSYVKGGGYTAMSDADYATSLADNAPDLTDKGITSVAGITP
                     VVLESKEQTDYVPNLTKLAEDEGCKMIVAVGFMLSDATYQVAKDHPDTNFMLIDAVPS
                     DPQTFEPLPELPNLVCYLFKEEQCGYLVGAIAGMATKNNKIGYIGGIAVPAVQRYEAG
                     FMAGIKTTNPTAYGTDGKNVSDVYAGSFGDQPKGKQIAQTMIASKCDILFHAAGATGN
                     GMFEAIKEAGGPDKGLWGIGVDVDMGKNPNLYPAGTLTSAIKHVDFATWEAVKAQADG
                     TFKPGTITLTLANGGVGYALDNVASVLTAEQIAKVNNLRQMMIDGTLVPPTDPKAVPA
                     WTAPTSY"
BASE COUNT          589 a          866 c          964 g          613 t
ORIGIN      
        1 ggagagggtc ctggacgcag acagccaggt caggccgact ggtgaacttg cagcagcgaa
       61 tcccgcaagg gagcctgcgt ctctgacggc aacgcagacg gtccgttcac gttggcccga
      121 ttcgatgcgg taggacacga tgacgcccat gccggtggca gcggcctgct tcacctcggg
      181 atcagcgatg cggttggtga cagaggccag gtcgtactcc gagtcggcga cagccgaacc
      241 cgatgtgttc aggatcgtca ggcgaagtcc ggaaggaagg tctcccccga ccagaggtgc
      301 gacgaggccg cctgaatgga gtgcctcagc atacgcgctg gagaggatca ccgcggctgc
      361 ctcgagacgt ccgacttcat ttatgtcgga ctgctgatgc acggacatga cctgtacgac
      421 gagggcgctt gtgaagacga ggccggcgac gacaaggatc acgctcagca cgcggagact
      481 gtacggacgg cttcgagtca tgcgtcgtct tctatgtagt atccgaagct gcgtgacgta
      541 cgcagcagat gcgctgcgta gctgagcttg cggcgtatgc ttgtgatatg aacgtcgacg
      601 acacgttcca cagcgtcagt ctcaccggtc gcccgcagga gctcctcccg ggagacgacc
      661 agacccttct gctcgaccag cgccgtcagg atgtgatact ccgccggcgt aaggtcgatc
      721 gacttcccac cgacactggc cgtcttccgc gcggagtcga tggcaatcgg gcctgccctg
      781 agggcctctt gccggaatgc ggggggacta gtctgcgacc ggcggagcat ggcgcccact
      841 tgcgcgacga gttcgtgcgg gttgaagggc ttgcgtatgt aggtgtccgc acccagtcca
      901 agggtcagga cgacgtcgct ctcggtggcc tttgcgctgc acaccacgat agacatggtg
      961 ttacacgagg ggcgtttgcg cacttccttg atgacgtcgc cccccgaaag ctccggcatc
     1021 atgaggtcca ggatgagaag gtccgggggc tgactctgcg catcgagcca ggaaaggaag
     1081 gtgacgggtg aggcaaacgg gagtggttcc atggaatctc tcttcaggta caggcagatc
     1141 agttcccgta tgtcgtcgtc atcctcgagc accgcaatgc ggttgatggt tccacctccc
     1201 cgggcgactg ttcacagtct attctcgcaa tgtagtatca gacccttgtc cgggcaagtc
     1261 aagaggctgt tctcgacgat tggaggcgag gcgactgcca cggatccata tcacgtcaag
     1321 gcatctgcag acggcacctg gatgatcacg ggacgcgtcg aatcagtcgg aacagggcac
     1381 ccatggcgtc cggcgtgtcc agggtccggc gtgacaacaa tgtgaaaagc tcggttgcat
     1441 tgccgcgtcg ggctatatac tgtggtcagc tgtagcaagc agcaaatccc tttccttttt
     1501 cattgccccg ggcttaaccc acctggggca tctccttttc cgggttcctg cccgtgtccc
     1561 tcgcccgtct gcagggagac gtcgacagat gcaaagaccc ccggcggtga agccgggggt
     1621 cgtgttggct tgctgtggag gaggttctag tagctggtcg gtgcagtcca agctggaaca
     1681 gccttgggat cggtcggcgg aaccagtgtg ccgtcgatca tcatctggcg aagattgttg
     1741 accttggcga tctgctcggc agtgaggacg ctggcaacgt tgtcgagagc gtagcccacg
     1801 ccgccgttgg ccagagtcag tgtgatcgtc ccgggcttga aggttccgtc agcctgcgcc
     1861 ttgacagctt cccaggtcgc gaagtcgacg tgcttgatgg cagaggtcag cgtgccggcc
     1921 gggtacaggt tcgggttctt gcccatgtcg acgtcgacgc caatgcccca cagacccttg
     1981 tcaggtccgc cagcttcttt gatggcttcg aacatgccgt tgccggttgc gccggcggcg
     2041 tggaacagga tgtcgcactt ggacgcgatc attgtctggg caatctgctt gcccttgggc
     2101 tggtcgccga agctgccggc gtagacgtcg gagacgttct tgccgtcagt accgtacgcg
     2161 gtcggattcg tggtcttgat gccggccatg aaacctgctt catagcgctg gacagctgga
     2221 acggcgatac caccgatgta gccgatcttg ttgttcttgg tggccatgcc ggcgatggcg
     2281 ccgacaaggt acccgcactg ctcctccttg aagaggtagc aaacgaggtt ggggagttcg
     2341 ggcagaggct cgaacgtctg aggatcgctg gggacagcgt cgatgagcat gaagttcgtg
     2401 tccgggtgat ccttggcgac ctggtaggtc gcatcagaaa gcatgaaccc gacagcgacg
     2461 atcatcttgc atccttcgtc ctcggcaagc ttggtgaggt tggggacgta gtccgtctgt
     2521 tccttggact caagaacgac cggagtgatg ccggcgacgg aagtgatgcc cttgtcggta
     2581 aggtcaggcg cattgtcggc aagtgacgtt gcatagtctg cgtcagacat ggcagtgtat
     2641 ccgccaccct tgacgtagga cttcttggcg gcccagatct cgaggccgcg cagggcggaa
     2701 tcgttgaatg actggtcgcc acggccaccg acgtcggtga caaggccgat cttgatctca
     2761 tcgacgacag gtgtcgcctt cttgcatccg gtgaagatgg acgcagcaag aactacaatc
     2821 agcagaacgg acagcagtct tctcatgtgt tctcctccag aacagattaa cctgatagta
     2881 accattgtag cacaaataca caatgagtct tctgaccgaa gtcagccggc aaaggccagc
     2941 cgccgggcga cacagtacgc cggtcaccac gcgagcacag gcccgcactt gtgcccaaag
     3001 tatacgccgt tgtccagaaa acgcatcaac cc
//
LOCUS       DATMZD010000033         3552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_101587,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000033 DATMZD010000000
VERSION     DATMZD010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3552
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101587"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="VN478_00710"
     CDS             <1..534
                     /locus_tag="VN478_00710"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002033512.1"
                     /note="catalyzes the formation of
                     4-imidazolone-5-propanoate from urocanate during histidine
                     metabolism; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HWQ21230.1"
                     /translation="ILKEFAYDKHLVKWIQMAHDQVQFQGLPARICWLGYGERERFGL
                     IINDLVKSGRISAPIVIGRDHLDCGSVASPNRETEKMMDGSDAIADWPILNALLNTIG
                     GATWVSVHHGGGVGIGYSIHAGMVIVADGTDAAAKRLSRVLTYDPGMGIVRHADAGYP
                     KAIQFARDHGIRMPSLK"
     gene            547..1233
                     /locus_tag="VN478_00715"
     CDS             547..1233
                     /locus_tag="VN478_00715"
                     /inference="COORDINATES: protein motif:HMM:NF025865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HWQ21231.1"
                     /translation="MEKGLNFISLIRDRHSIRNYSQYALTDADRTQILETVAGAVPLD
                     SGIHLDWRIGDDAPMGASGLLYAECGTSAEELVEYGYRGEQAVLGLTAEGWGTCWYGM
                     AHMPGSPCSILLGKPGTPGVRTAVLGVLSRGHTRKSLEQLVTSGISAESSPLVRTVLE
                     SARLAPSAMNRQTWTFEVASDTRVVIKGGIGRFPDLGICLANAMVTARQLAGSATVTR
                     LDSGEYGVSW"
     gene            1458..3179
                     /locus_tag="VN478_00720"
     CDS             1458..3179
                     /locus_tag="VN478_00720"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWQ21232.1"
                     /translation="MKRLFREAGSLTWLAAGAIICAVIVSVATVALADVFGRALSLVV
                     AGAGVTALHQVIIVGVAAGAASIGCTFLGKLLAGHFSERLQRRLRDRAVERLAHATAA
                     AMHGEHSGDVQSRFSSDMVLLEQLVRTDVLQFVSQGLTALLAAAYMLSRNWLLTVVAV
                     APTPILLLVASYLTKPLGPLMTSAQEALGQAGVATQEAVSGAEVIRAMSIGAAITVRH
                     EHSLATWQERSISAAHQVAKLYSTGVTLSIMPFIAVFAVGGYLALAHRIELGLLFSFV
                     QLVNYLSSPVQEMPRLLGRIRQGVAAAHRVVELLDVPVERTAGIRGSDTADEVICLEN
                     VSFAYPGTTEPSLRDISLTVKRGQKVALVGGSGSGKSTVLRLVIGDYSPDTGTVLVNG
                     LSVQDWDLAALRSVIAVVDQEAFLFGDSVADNVRAGRLGATQAQVDEALNVAAADFVA
                     EMAQGQGTLVGDMGGRLSGGQRQRLALARALVKEAPLIVLDEATSALDNELERRVYAE
                     LLEHYPDKTVVAVAHRLTTIQDADMIYVFDHGRIVEEGTHDSLLAADGRYALLWNLQQ
                     TQENNHE"
     gene            3172..>3552
                     /locus_tag="VN478_00725"
     CDS             3172..>3552
                     /locus_tag="VN478_00725"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HWQ21233.1"
                     /translation="MSSWRALRRLFSCTGDAAPQYISGIAGLGLINYAFNAMFAISLS
                     MFTRGLMNGSRSVLNRSVLIFLLVGVAGAVAVLFAGRALVIGAVRSERHVRSAVFAAV
                     NRLPLTTIERLGPGEVLSRLTNDTA"
BASE COUNT          670 a         1094 c         1070 g          718 t
ORIGIN      
        1 atcctgaagg agttcgccta tgacaagcac ctcgtaaaat ggatccagat ggcacacgac
       61 caggtccagt tccagggcct gcctgccagg atctgctggc tcgggtacgg cgaacgcgaa
      121 cggttcggcc tcatcatcaa cgatctcgtg aaatccggca ggatctccgc acccatcgtt
      181 atcggacgcg accacctcga ctgtggttcc gtcgcctcac ccaaccgtga aaccgagaag
      241 atgatggacg gcagcgatgc cattgccgac tggcccatcc tgaatgctct tctcaatacg
      301 atcggaggag ctacctgggt ttcggtccat cacggcggag gcgtcggcat cggctactcg
      361 atccacgccg ggatggtcat cgtcgctgac ggcaccgatg cagcagccaa gcgtctgtca
      421 cgcgttctca cgtatgaccc gggcatgggg atcgtgcggc atgccgacgc agggtacccg
      481 aaagccattc agtttgccag ggaccacggc atccgcatgc catcactcaa gtagggaact
      541 cagtcaatgg aaaagggact caacttcatc agtctgatac gcgaccgcca ttcgatacgc
      601 aactactcgc agtatgcgct gactgacgcc gacaggactc agatcctcga gacggtcgcc
      661 ggcgcagtcc cgctcgacag cggcattcat cttgactgga ggatcggcga cgacgctccg
      721 atgggggcca gcgggctcct gtacgcagag tgcggcacgt cagccgagga gctggtcgaa
      781 tatggctaca ggggcgagca ggctgtactc ggcctgaccg cagagggatg gggaacatgc
      841 tggtatggca tggcacacat gcctggcagc ccctgttcga tcctccttgg caaacccggc
      901 acaccgggcg tgcgaactgc cgtgttgggc gtgttgtcac gcggacacac ccgcaagtcg
      961 cttgaacagt tggtgacgag tggcatctct gcggagtcct ctccgctcgt gcgtaccgtt
     1021 ctggagtccg cccgccttgc cccctccgcc atgaaccgac agacctggac cttcgaagtt
     1081 gcatcggata cacgagttgt catcaaaggc ggcatcggcc gctttcctga cctgggcatc
     1141 tgcctggcga acgcgatggt cacggcacgc cagctggccg gcagtgccac tgtcactcgc
     1201 ctggactcag gcgaatacgg cgtctcctgg tagtctgcag gcaggctcaa ggccggcagc
     1261 acgtgacgag ccaggaatac cgtacggtga aaggcaaggc ttgatccacc gatattgcgt
     1321 ttattcgtac tacgatatat acttctcaca tgcagatcag aagaagacag gaaccagtcg
     1381 atgaacgttg agtcacgagc agttgctgtc ctgacgactc ccctcgacac aaggcgagta
     1441 cggtcggacg ttttctcatg aagcgcctgt ttcgagaggc cggcagcctg acctggctgg
     1501 cagctggtgc catcatctgt gccgtcatcg tatcagtggc caccgtggcc cttgccgacg
     1561 tcttcggtcg tgcactgtcg ctcgtcgttg ccggcgccgg cgtgactgct ctccaccagg
     1621 tcatcatcgt gggggtcgcc gccggagctg cttcaatagg ctgcaccttc ctgggcaagc
     1681 ttctcgccgg gcacttcagc gagcgccttc agcgacgtct ccgtgaccgc gccgtggagc
     1741 gcctcgcaca tgcgactgcc gctgcaatgc acggtgagca ctcgggcgat gtccagtcac
     1801 gcttctcgag cgatatggtt ctgctcgaac agctcgtcag gacggacgtc ctccagttcg
     1861 tatcacaggg tctcactgcc cttctcgcgg cagcctacat gctttccagg aactggctgc
     1921 ttacggtcgt ggccgtcgcg ccgacaccga tcctccttct cgtggcgtcg tatctcacga
     1981 agcctctcgg gcctctcatg acatctgccc aggaagctct cggacaagcc ggcgtcgcta
     2041 ctcaggaagc agtgtcgggc gcagaggtca tccgtgcgat gagtatcggt gcagccataa
     2101 ccgttcgcca cgagcactcc ctggcaacgt ggcaggaacg gagtatttcc gctgcacatc
     2161 aggtcgcgaa gctgtattcg acgggcgtga cgctctcgat catgccgttc atcgcggtct
     2221 ttgcagtcgg aggttacctc gctctggctc accgcatcga gctggggctg ttgttctcgt
     2281 tcgttcagct ggtcaactac ctctcttctc ccgttcagga gatgccgcga ctgctcggcc
     2341 gcatccggca gggtgtcgct gcggcacacc gcgtggtgga actgctcgac gttccagtgg
     2401 agcgcaccgc agggatacgt gggtctgaca ccgctgatga ggtgatctgt ctcgagaacg
     2461 tgtcattcgc ctatcccggt accacagagc catcgctgag agacatctca ctgaccgtga
     2521 agcgtggaca gaaggtcgca ctggtcggcg gcagcggttc gggcaagtcg acagtcctcc
     2581 gactcgtcat tggtgactac tcccccgata cgggcacggt gcttgtcaac ggactttcag
     2641 ttcaagactg ggatcttgct gcacttcggt ctgtcatcgc ggtagtcgat caggaggcat
     2701 tcctctttgg tgacagcgtc gccgataatg tccgtgccgg acgcctgggc gcgacccagg
     2761 cccaggtcga cgaggccctg aacgtagcag ccgccgactt cgtggccgaa atggcacagg
     2821 gtcagggcac actggtcgga gacatgggag gtcggctgtc aggcggccag cggcaacgtc
     2881 tggcgctggc acgcgcactc gtcaaggaag cacccctgat cgtccttgac gaagccacgt
     2941 ctgcactcga caacgagctg gaacggcgtg tgtatgctga gctcctggaa cactatccgg
     3001 acaagaccgt cgtcgccgtc gcccacaggc tgacgaccat ccaggatgct gacatgatct
     3061 acgtattcga tcacggccgc atcgtcgaag agggaacaca cgacagtctt ctggcagcgg
     3121 atggacgcta tgcactgtta tggaacctgc agcagacaca ggagaacaac catgagtagc
     3181 tggagagctc tgaggagact gttctcgtgc acgggcgacg ctgctcctca gtacatctcg
     3241 ggtattgccg ggctgggcct catcaactac gcgttcaacg ccatgttcgc catttcgctg
     3301 tcgatgttca cacgcgggct gatgaatggc agccgttcgg tgctgaaccg ctcggtcctg
     3361 atcttcctgc tcgtcggggt tgctggagct gtggccgtcc tgtttgccgg acgagcactg
     3421 gtgatcggtg cagtgcggtc tgaacgtcac gtgcggagtg cggtgtttgc cgctgtcaat
     3481 cgtctcccac tgacgaccat cgaacggcta ggaccgggag aagtcctttc gcggctcacg
     3541 aacgataccg ca
//
LOCUS       DATMZD010000034         2024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_20915,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000034 DATMZD010000000
VERSION     DATMZD010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20915"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1501)
                     /locus_tag="VN478_00730"
     CDS             complement(<1..1501)
                     /locus_tag="VN478_00730"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWQ21234.1"
                     /translation="MRVVGDSVLRDRYDVVVVGAGLGGLTAGALLARRGLSVLIIDQQ
                     DRPGGSCTSFKRDGIVYDVGTAMIYGFGETGFRPFRFIMNELEEPIDIVAQPTLARMT
                     IEGQKVVFWPDLERFENELGDMFPEEREGIRSFYRELYGMYKNIVLRNEVIVPPSEFS
                     VRQGLRSLLSSPVAMLKMQKLLTVSVKSLLDKHFHTKPIIDFFDKLCSAYDYCTAAET
                     PAVLAATMFIDNHVGGVYYPAGGAQNLPNTMEKAFERDGGQMLYQKKVDEILIRGDTA
                     YGVRLTDGTEILADSVIADSTVWNIYGKLVKAEHIAPERLAWAKSLVSTYPSMTMYML
                     VDAGALPADTFPWEIFIENRALIDSSDLTLYTNSLVDRTLAPEGQLVVMAIAPNMLSW
                     PSSDSPEYRSETYERQKSQEADRMLAQIESHFPGFRRHIRTLIVATPTTIERYLLKNG
                     GAVGGPKNMLGQQMLKRLHARSEWKNLYFCGDSTVMGTGAPATAVSGVGA"
     gene            1687..>2024
                     /locus_tag="VN478_00735"
     CDS             1687..>2024
                     /locus_tag="VN478_00735"
                     /inference="COORDINATES: protein motif:HMM:NF019450.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper amine oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HWQ21235.1"
                     /translation="MRHTQRSIAILIVLAVVLALPVMPSSVRGQTTGTQTTIVLQVGK
                     PSMTVNGVSRAIDPQGTVPVIIESRTFLPIRAIVEALGGTIAWTAAEQKVTIIMGSDV
                     LELFIGKNTAR"
BASE COUNT          361 a          622 c          630 g          411 t
ORIGIN      
        1 ccgccccgac gccggagacg gcggtggccg gcgcccccgt tcccatgacc gtcgaatccc
       61 cgcagaagta caggttcttc cattcgctgc gggcgtgcag gcgcttcagc atctgctgtc
      121 cgagcatgtt cttcgggccg ccgacggcgc ccccgttctt gagcaggtag cgttcgatgg
      181 tcgtgggcgt cgccacgata agcgtccgga tgtggcgtcg aaaaccgggg aagtgcgact
      241 cgatctgtgc cagcatgcgg tcggcctcct ggctcttctg ccgttcgtat gtctcggaac
      301 gatattcagg gctgtccgac gagggccagg agagcatatt gggagcgata gccatgacga
      361 ccagttgacc ctcgggcgcc agagtgcggt cgacgagcga gttcgtgtac aacgtcaggt
      421 ccgaagagtc gatcaatgcg cggttctcga tgaagatctc ccatgggaac gtgtcggcgg
      481 ggagagcccc cgcgtcgacc agcatgtaca tggtcatgct ggggtaggtc gacacgaggg
      541 acttggccca ggcaagccgc tccggcgcga tgtgttcggc cttcacgagc ttgccgtaga
      601 tgttccagac ggtcgagtct gcgatcacgc tgtccgcgag gatctcggtg ccgtctgtga
      661 gccggacgcc ataggcggtg tctcccctga tgaggatctc gtcgaccttc ttctggtaca
      721 gcatctggcc gccgtcgcgc tcgaatgcct tctccatcgt attgggcagg ttttgcgctc
      781 cgccggccgg gtagtagacg cccccgacgt gattgtcgat gaacatggtg gctgccagga
      841 cggcaggggt ctcggcggct gtgcagtagt catacgccga acacagcttg tcgaagaagt
      901 cgatgatggg cttggtatgg aagtgcttgt ccagcagcga tttgacgctg acggtcagca
      961 gcttctgcat cttcagcatg gcgacgggac tgctgagcag gctgcgcaga ccctgccgca
     1021 cagagaactc tgacggaggg acaatgacct cattgcgcag cacgatgttc ttgtacatgc
     1081 cgtagagctc gcggtagaaa ctcctgatgc cttcccgttc ctcggggaac atgtcgccca
     1141 gctcgttctc gaaccgctcc aggtcgggcc agaagacgac cttctgtccc tcgatcgtca
     1201 tgcgcgccag cgtcggctgc gcgacgatgt cgatgggctc ctcgagttcg ttcatgatga
     1261 accggaatgg cctgaacccc gtctcgccga agccgtagat catggcagtg ccgacatcat
     1321 agacgatgcc gtcgcgcttg aacgacgtgc atgatccacc gggcctgtcc tgctggtcga
     1381 tgatgaggac ggacaggccc cgcctggcca gtagagcacc tgcagttagt ccgcctaggc
     1441 ccgccccgac gacgaccacg tcgtaccggt cgcggagtac cgaatcgccc actactctca
     1501 tggtctcctg tacctcctgt ctgcgtttga gaatctcgcg catggcgtgc tgagtacgta
     1561 tcttaccaca tgtgcggaca atcagaatgt ccgttgttgc atcctgtggc atgtccttat
     1621 catatgacca tgtgtggagc ccgccgcgaa cgtgccgggg gtcgctccac tggctgggag
     1681 gccgccatga gacatacaca gcgttccatc gccatactga tcgtccttgc cgtcgtgctt
     1741 gctcttccag tcatgccctc cagcgtgcgc gggcagacca cggggacgca gacaaccatc
     1801 gtcctccagg tcggcaagcc gtcgatgaca gtgaacggcg tgagccgggc catcgatcca
     1861 caggggacag tacctgtcat catcgagagc cggacgttcc tgcccatccg ggccatcgtc
     1921 gaggcccttg gcggcacgat cgcctggacc gctgcggagc agaaggtcac catcatcatg
     1981 ggatcggacg tcttggagct cttcatcggc aagaacacag ctcg
//
LOCUS       DATMZD010000035         4406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_41017,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000035 DATMZD010000000
VERSION     DATMZD010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4406
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41017"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="VN478_00740"
     CDS             <1..677
                     /locus_tag="VN478_00740"
                     /inference="COORDINATES: protein motif:HMM:NF014879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HWQ21236.1"
                     /translation="ARVVAEKADLGVAHDGDGDRVIMVDRDGRRVSGDVMLYILALGL
                     AKEGRLTGNTVVGTVLTNLGLEKALELQGIRLERTSVGDRYILDRLNEGGFVLGGEES
                     GHIINTYQNVTGDGLATSLAVLGYLARTGRDISEIVDEVELYPQIAENVRVKDKSIAS
                     CPEFVAAVEAARLELGNAARLVVRPSGTEPLVRIFLEGKDTERLVALADRLKGVLLSA
                     GQQGEE"
     gene            679..2505
                     /gene="glmS"
                     /locus_tag="VN478_00745"
     CDS             679..2505
                     /gene="glmS"
                     /locus_tag="VN478_00745"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HWQ21237.1"
                     /translation="MCGIIGYVGPRKVVPVIIEGLRKLEYRGYDSAGMAILEKGELVV
                     VKKRGKLKALEEAIEGNNYTATTGMGHTRWATHGRVEDRNAHPHVDCKNAVAVIHNGI
                     IENYQVLRDDLIRKGHVFVSETDTEVIAHLMEEQIDAGADLLDAARKTALQLDGAYAF
                     LVLARAFPGRIVAVRKLSPLVLGVGDGEMILGSDTPALLEYTHRIIPMHDGDVVEINE
                     GGYAFYSLDDGRSLDRTPMTVQWNASAAEKQGYKHFMLKEIHEQPVVIRDTLYGRIDI
                     DSGKVDLEEFESQPVPERISIVAAGTSYHAARVGKYLLEELARIPTEVSFSSEYRYQR
                     PIVRPGTMGIAVTQSGETIDTLAALRLQRDLGAKVVAITNRPESTVSRESDVTFVTQA
                     GLEIGVAATKSFMAQLIAFYLMALNLGQRNGVLDAETSVHYASQLSSLSQKCEQVLEY
                     AGIMETIARKFYKVHDMIYVGRGVNYPIALEGALKMKEISYIHAEGYAAGELKHGPIA
                     LLDEDVPVVGILPQGVLYEKIYSNLQESIARDSPLVVLADKKDRRAQDIATDFIPMPS
                     VDELFSPVVYTLPLQLLAYYTADGKGLDVDQPRNLAKSVTVE"
     gene            2586..2662
                     /locus_tag="VN478_00750"
     tRNA            2586..2662
                     /locus_tag="VN478_00750"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2620..2622,aa:Arg,seq:ccg)
     gene            2860..3690
                     /locus_tag="VN478_00755"
     CDS             2860..3690
                     /locus_tag="VN478_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013497080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HWQ21238.1"
                     /translation="MGRRRLFCDISPTTYRLSVLKGILLRDLQDVVRAQRFADTRTEA
                     LLPVLVYRNSSLMRRVLGNVDPVLQENKVTNLRLSAPRVTNVLIRPGETFSFWKLAGR
                     CTEREGYKKGLVISSGRLSEGIGGGLCQFTNLIHFLTLHSPMEIVEHHHHDEMDLFPD
                     FGRQIPFGTGTSITYNYLDYRFKNTTDTTFQLIAFVTDRYLCGELRADKPLAVRYHIR
                     TEDECFSREGDVVYRNGLVFREQIDAKTGNLLRRELIKKNHARVMYDVDDDRILRNAR
                     "
     gene            3956..>4406
                     /locus_tag="VN478_00760"
     CDS             3956..>4406
                     /locus_tag="VN478_00760"
                     /inference="COORDINATES: protein motif:HMM:NF013878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HWQ21239.1"
                     /translation="MKKLVIAEKPSVAEQLAAVIDHCKKSRDYYEGEHYIVSWAVGHL
                     VGLAEPEDYDKKYKQWLLSYLPIIPEAFQFSVLEGAEERFNALKRLIVSKDVDEVINA
                     CDAGREGELIFRNIYAQAGGKKPSSRLWLSSYTDESIRDGFKHIRPES"
BASE COUNT          966 a         1168 c         1363 g          909 t
ORIGIN      
        1 gagcccgcgt ggttgccgag aaggctgatt tgggcgttgc ccacgatggt gacggcgacc
       61 gcgtcatcat ggtcgatcgt gacggccgtc gcgtgtctgg ggacgtcatg ctgtacattc
      121 tggctctggg actggcaaaa gagggtcgac tcactggaaa caccgtcgtt ggaacggttc
      181 tcaccaacct tgggctggag aaggctctgg aactacaggg aatcagactg gaacggacga
      241 gtgtcggaga ccgctacata ctggaccgcc tcaatgaagg cggcttcgtg ctcggcggcg
      301 aggagtcggg gcacatcatc aatacatatc agaatgtgac aggcgatggt ctcgcgacgt
      361 ctttggccgt gcttgggtac ctcgccagga caggacggga catttcggag atcgttgacg
      421 aagtcgagct ctaccctcag atcgctgaga atgtaagggt gaaagacaag tccattgctt
      481 catgcccgga gtttgttgcc gcggtcgaag cagcacgact ggagttgggc aatgctgcca
      541 ggctggtcgt acggccttcg gggacggagc ctctagtccg catctttctc gaggggaagg
      601 acactgagcg ccttgtggcg ctcgccgacc ggctcaaggg agtcctgttg tcggctggtc
      661 agcagggaga ggagtgacat gtgcggaatc atagggtatg ttggaccacg taaggtagtc
      721 cctgtcatca tcgagggtct gcgcaagctc gagtatcgag gatacgactc cgcaggcatg
      781 gcaattctcg agaaggggga gctcgtcgtc gtcaagaagc gcggaaagct caaagctctc
      841 gaggaggcta tcgaggggaa caactacact gcgaccacgg gaatggggca cacaagatgg
      901 gccacgcacg gcagggttga ggatagaaat gcgcatccac acgtggactg caaaaacgcg
      961 gtcgccgtca ttcataatgg cattatcgag aactaccagg tgctccggga tgacctgatc
     1021 cgaaaaggac acgtcttcgt ctccgagacc gataccgagg tcatcgccca tctcatggag
     1081 gagcagatcg atgccggtgc agaccttctc gatgcggcgc gcaaaaccgc gctgcaactg
     1141 gatggagcct atgcgtttct ggttctggcc agggctttcc ctggtcgaat cgtggcagtg
     1201 cgcaagctgt cgccccttgt gcttggcgtc ggcgacgggg agatgatcct cggttccgac
     1261 acgccggcac ttctggagta tacgcaccgg atcatcccga tgcacgacgg cgacgtcgtc
     1321 gagatcaatg aagggggata tgccttctac tcgctggatg atgggcgttc cctcgaccgg
     1381 acaccgatga cagttcagtg gaatgcatcg gctgccgaga aacagggcta caagcacttc
     1441 atgctcaagg agatccatga acagccagtc gtcatcaggg atacactcta tggtcggatc
     1501 gacatcgaca gcgggaaggt cgacctcgag gaattcgaga gccagccggt accagagcgc
     1561 atatcgattg tcgctgcggg gacgtcgtac catgccgcgc gcgtaggcaa gtaccttctc
     1621 gaagaactgg cacgcatccc gaccgaggta agtttcagca gcgagtaccg ctaccaacgg
     1681 ccgatcgtcc ggccagggac catgggaata gcggtcacac agtcgggcga gacgattgac
     1741 acgcttgctg cgcttcgact tcaacgggac ctcggcgcga aggtcgtagc catcacaaac
     1801 cggcccgaga gtacggtttc acgcgagtca gacgtgacgt tcgtcactca ggctggtctc
     1861 gagatcggtg tcgcggccac caagtcgttc atggcccagc tcattgcttt ctacctgatg
     1921 gctctcaatc tcggccagcg caacggcgtg cttgatgcgg agacgtcagt gcattatgcg
     1981 tctcaactct cgagtctgag ccagaagtgc gagcaggttc tcgaatacgc cggcatcatg
     2041 gagacgatcg cgcgcaagtt ctacaaagtg catgacatga tctatgttgg gcgcggcgtc
     2101 aactacccca tagcgttgga gggggcactc aagatgaagg agatcagcta catccatgct
     2161 gagggctatg cggcgggaga actcaagcat ggtcccatcg ccctgctgga cgaggacgtg
     2221 cccgtggtgg gcatccttcc gcagggagtg ctttacgaga agatctactc gaatctgcag
     2281 gagtccatcg ctcgcgattc gccgctcgtg gtgctggcag acaagaagga caggcgagcc
     2341 caggatattg ccacggactt catccccatg ccgtcggtcg acgagttgtt ctcccctgtt
     2401 gtctacacac tgccacttca gttgctggca tactacacgg ctgacggcaa gggcctggac
     2461 gtagatcagc cgcggaacct ggcaaagagc gtcaccgtgg agtagctgag gcctgttttg
     2521 tgaggcgctg agccaaaggc cgaccttgta cagggtcggc ctttctgcta tacttggcga
     2581 cgtgcgcgcc cgtggcccaa ctggataagg cgatggcctc cggagccgta gattacaggt
     2641 tcaaatcctg tcgggcgcac caacagtgta gctatcagtc cagacggttc tcgaagttct
     2701 caggggacaa tcgctctgcc gtcggcaatt cggtccgccc cggaaatcgg ccaatcacac
     2761 acttcggaga tcacgggtgc agaggacagg cctgcacgcg tggactctcg tggtatgctg
     2821 caagaaggat tggttgtgtt cttgtgcgag gcgggcatca tgggtcggag aaggctcttc
     2881 tgtgacatat cgccgacaac gtacaggttg tccgttctca agggtatcct tctgagagac
     2941 ttgcaggacg tcgtccgcgc tcagagattc gcggacacca ggacagaagc cctgttgcct
     3001 gtcctggtct accgaaacag ttcgctgatg cgcagagtgc ttgggaatgt ggatcctgtg
     3061 ctgcaggaga acaaggtgac caatctgcgc ttgtccgcgc cgagagtcac gaatgtcctg
     3121 attcgtccgg gagagacctt ctccttctgg aagctggctg gaaggtgcac tgagagggaa
     3181 ggctacaaga agggactcgt tatcagctcc ggaagactgt cggagggaat cggtgggggg
     3241 ttgtgccagt tcacgaatct cattcacttt ctgacactgc attctcccat ggagattgtc
     3301 gaacaccacc atcatgacga aatggatctg ttccctgact ttggcaggca gattcctttt
     3361 gggacaggga catcgattac gtacaactat ctggactatc gcttcaagaa caccacggat
     3421 acgacgtttc agctcattgc atttgtcacg gacagatacc tgtgcggaga actgcgcgcg
     3481 gacaagcctt tggccgtccg atatcacatc aggacagaag atgagtgttt ctcgagggaa
     3541 ggagatgtcg tgtatagaaa cggcctggtc ttcagggagc agatagatgc gaagacgggg
     3601 aatctcctgc gaagggaact gatcaagaag aatcatgcga gggtcatgta cgatgtcgac
     3661 gacgacagga ttctccgcaa tgcgaggtga gcgcttctgc ggcagtcacg tccggaagga
     3721 gcgacagcga tgacgtccgt gccacctctt ctatgtcctg aggccgcttt ctttcgcgca
     3781 tccggttgaa tgaactcgcg ttcgtttcag cgagtgtgcg ggcggccgag gagtcggctc
     3841 caagccgatc cagctgtcga ccggaccctg tggactgaga agccgcagcc acttgcccac
     3901 ggtcttttgc gtgtataatg agggccgtca ttgtcgaggc atccaaggag tagtcgtgaa
     3961 gaagctcgtc atagcagaaa agccgagtgt agcggagcag cttgctgcgg tcatcgacca
     4021 ctgcaagaag agccgtgact attatgaagg tgaacactac atagtctcat gggcagtcgg
     4081 ccaccttgtt gggttggccg aaccggaaga ctacgacaag aagtacaaac aatggctgtt
     4141 gtcgtacttg ccgatcattc ccgaagcgtt ccagttctct gtgcttgagg gcgccgagga
     4201 gcgcttcaat gcgctcaagc ggctgatcgt ctccaaggat gtggacgaag tcatcaatgc
     4261 gtgcgatgca ggccgtgagg gcgagctgat ctttcgaaac atctatgcgc aggccggggg
     4321 gaagaaaccc tcttcgcgcc tctggctgtc ctcctatacg gatgagagca tccgtgacgg
     4381 attcaaacac atccgtcctg agtctg
//
LOCUS       DATMZD010000036         2650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_10982,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000036 DATMZD010000000
VERSION     DATMZD010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10982"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VN478_00765"
     CDS             <1..250
                     /locus_tag="VN478_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013931541.1"
                     /note="catalyzes the removal of amino acids from the N
                     termini of peptides; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HWQ21240.1"
                     /translation="AETLHKVLGMLDEARVPWQVAEMGKVDVGGGGTVAKFLSKRGIE
                     TLDAGPALLSMHAPQELASKADLYACYLAYKTFLEKAR"
     gene            392..838
                     /gene="rpsF"
                     /locus_tag="VN478_00770"
     CDS             392..838
                     /gene="rpsF"
                     /locus_tag="VN478_00770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HWQ21241.1"
                     /translation="MREFELMFIVDGTMPEEEATAVAATVQSFISSKGTILKADPWGR
                     RRMAYPINKKQDGYYWVVALQCEPGDVDVLKQQLRVNESIIRWMVTRPEHKKTVLPVE
                     AVVPVVPQETAPAPEALPEAAPVPEQAPEAAPVSAPASEPTPAPEA"
     gene            841..1314
                     /locus_tag="VN478_00775"
     CDS             841..1314
                     /locus_tag="VN478_00775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HWQ21242.1"
                     /translation="MNDLNQVVLTGRITRDPDIRYTPSGKKTARVSIVVNRSWNASKD
                     PSVRDWKEEAFFVDVNAWTFVADKIEKSAEKGLRVLVTGRLSIRDYTANDGTKKRATE
                     IVATTFEVIGAPQNRQVSGEPTEAPGEDDLLKDFEVPEGSLQQGSGSEDHDVPFN"
     gene            1334..1603
                     /gene="rpsR"
                     /locus_tag="VN478_00780"
     CDS             1334..1603
                     /gene="rpsR"
                     /locus_tag="VN478_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014452868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HWQ21243.1"
                     /translation="MPNAPFKKPSKTGKKKSFYFRARKKFCPFCADKTDDIDYKDASR
                     LRRFVSDKGKILPRRATGVCAKHQRALAGAVKRARVMALLPFVNR"
     gene            1617..2072
                     /gene="rplI"
                     /locus_tag="VN478_00785"
     CDS             1617..2072
                     /gene="rplI"
                     /locus_tag="VN478_00785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HWQ21244.1"
                     /translation="MDKKVILLADVKGVGHKNDVVSVSVGYAANYLLPQHLAADATPD
                     RVAVLSKHKMAASQEKDIALSRAQELAARLEGQSVTVRAKATPQGRLYGAITPDEIAA
                     AIGSQLSVAVDKKTVGTEEPIKALGVYAVALKLNPRVTALVSVTVAEAV"
     gene            2075..>2650
                     /locus_tag="VN478_00790"
     CDS             2075..>2650
                     /locus_tag="VN478_00790"
                     /inference="COORDINATES: protein motif:HMM:NF012974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HWQ21245.1"
                     /translation="MVDRSDAQVDETRPRMGGNESGANPSSVVPPYDMGAEQSLLGSF
                     IVSNATVDEVMDYLKPDDFYFDEHVAICDSIFRLRASNKPVDVIVLSNDIKSRGNLDR
                     IGGVAYLAKLTELVPTTQNVKYYAEIIKESSIKRSIIRAGRDIYEMGFSPAVTAMELI
                     DRTEKLIYEASQGMVRGDFQPLERVLQDTFLV"
BASE COUNT          605 a          753 c          763 g          529 t
ORIGIN      
        1 tgccgagact ctccacaagg tacttgggat gcttgatgag gcacgagtcc catggcaagt
       61 ggccgagatg ggcaaagttg atgtcggcgg cggcggcacc gtggcgaagt tcctgtcgaa
      121 gcgcggtatc gagacactgg acgcaggtcc tgcgctcttg tcgatgcatg ctccgcagga
      181 actggcgagc aaggcagatc tgtatgcctg ctacctggcc tacaagacgt tcctggagaa
      241 ggcccgctga ttcgtcatac atgcgattga tggcacggcg atgcacccgt gaaggattgt
      301 cccggttgca tcgccgtgcc tcctgtatat actatttctg ctctctgccg tcgtgaagac
      361 ggccgaaatc taagtccaaa gggaggaact cttgagagag tttgaattga tgttcattgt
      421 ggacgggacg atgccggagg aagaagccac ggccgttgct gctaccgttc agtccttcat
      481 ctcgtcgaag ggcacgattc taaaggctga cccatggggt cgtcgtcgca tggcctatcc
      541 aatcaacaag aagcaggacg ggtactactg ggtagtcgca ctccagtgtg agcctggtga
      601 cgtggacgtc ctcaagcagc agctgcgcgt gaacgaaagc atcatccgat ggatggttac
      661 gcggcccgag cacaagaaga ccgtcctccc cgtcgaggca gtcgtcccag ttgtcccgca
      721 agagactgcg cccgctcccg aggccctccc ggaggctgct ccagtacccg agcaggcacc
      781 cgaagccgct ccagtatcag cacctgcatc tgaacccacc cctgcacctg aggcgtagga
      841 atgaacgacc tcaaccaggt tgtcctcaca ggcaggatta ccagggatcc agatatccgg
      901 tacacaccgt cgggcaagaa gaccgctcgt gtctcgatcg ttgtgaaccg gagctggaac
      961 gcgtcgaagg atccgtccgt tcgtgattgg aaggaagaag ctttcttcgt tgacgtcaac
     1021 gcatggactt tcgtggctga caagatcgag aagtcagccg agaagggctt gcgtgttctc
     1081 gtgacaggac gcctgtccat cagagactac actgccaacg atggcaccaa gaagcgtgca
     1141 accgagatcg tggctacgac attcgaggtc atcggcgctc ctcagaaccg tcaggtttcg
     1201 ggtgaaccca ccgaggcacc tggcgaagac gaccttctca aggactttga agtccccgaa
     1261 ggatcgttgc agcagggttc cgggtctgaa gaccacgacg tgccattcaa ctgagatgaa
     1321 ggagtagact actatgccaa acgcaccatt caagaagcca agcaagaccg gcaagaagaa
     1381 gtctttctat tttcgtgcac ggaagaagtt ctgtccgttc tgcgctgaca agaccgacga
     1441 tatagactac aaggatgctt cacggcttcg ccgtttcgta agcgacaagg gcaagattct
     1501 tcctcgccga gcaaccggag tgtgcgcgaa gcaccagcgc gcccttgcgg gcgctgtcaa
     1561 gagagcccgc gtgatggccc tcctgccatt tgtgaatcgg tagcacaacg ctgaccatgg
     1621 acaagaaggt aatactgctc gccgacgtca agggcgttgg ccacaagaat gatgtggtca
     1681 gcgtttcggt aggctacgct gccaattacc tccttcccca gcatcttgct gctgatgcga
     1741 cacccgacag agtagccgtc ctcagcaagc acaagatggc ggcctctcag gagaaggata
     1801 ttgcgctctc gcgcgcgcag gagctggcag cgaggcttga gggccagtca gtcaccgttc
     1861 gcgcaaaggc gaccccacaa ggaagactgt acggcgccat tactccggac gagatcgcag
     1921 ctgcgatcgg cagtcaactc agcgttgcgg tggacaagaa gaccgttggc actgaggaac
     1981 ccattaaggc gctcggcgtg tacgccgttg ccttgaagct gaacccccgg gtcaccgccc
     2041 tcgtttcagt cactgttgcg gaagcagtct gaggatggtc gatcggagtg atgcccaggt
     2101 agacgaaacc cgtccacgga tgggcgggaa cgagtctggc gcaaatccga gcagtgtcgt
     2161 tccaccgtat gacatgggag ccgagcagtc tctgcttggc tccttcatcg tgtcaaatgc
     2221 caccgttgac gaggtcatgg actatctcaa acctgacgac ttctatttcg atgagcatgt
     2281 cgccatctgt gattctatct tccgtctgcg agcatccaac aagcctgtcg acgtcattgt
     2341 cctcagcaac gacatcaaga gccgcggcaa tctggatcgc atcggcggtg ttgcctatct
     2401 ggccaaactc accgagcttg tcccgacgac ccagaatgtc aagtactatg ccgagatcat
     2461 caaggagtca tcgatcaagc gaagtatcat ccgtgccggg cgagatatct atgaaatggg
     2521 attctcacct gccgtcaccg caatggaact catcgacagg actgagaagc tcatctacga
     2581 ggcatctcag ggcatggtgc gcggcgactt ccagccactg gaacgtgtcc ttcaagacac
     2641 gttcctggtg
//
LOCUS       DATMZD010000037         3269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_81670,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000037 DATMZD010000000
VERSION     DATMZD010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81670"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..1012)
                     /locus_tag="VN478_00795"
     CDS             complement(2..1012)
                     /locus_tag="VN478_00795"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWQ21246.1"
                     /translation="MRARPLAVGLIVILAANLFAGCRAPGATVVCYTSVDQEYAAPLF
                     KAFEARTGVRALVVYDTEAAKTTGLVNRLVAEQAHPVCDVFWNNETTQMSTLVQNNVL
                     RQLPEGAGQGILAQYRDPDGLWAGHAARFRVLLLNTKVFKGPSPTSVLDLASSAYARA
                     SVGMAMPLFGTTAAHAAALYSVLGAARAYAFFQQVSASGVRIVDGNATVRDLVGQGVL
                     TWGITDSDDALVAVKRGDAVSVVVPDQDGMGTLVIPSSVAVIRGGPHPDEADSLAEYL
                     LSEDVEATLLKEGYCQLSTRREGGIAPLTGTSIKGMDVDPRSIQISMMTAKVDLASIF
                     GQ"
     gene            complement(1018..1491)
                     /locus_tag="VN478_00800"
     CDS             complement(1018..1491)
                     /locus_tag="VN478_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21247.1"
                     /translation="MSPYGGPGSGVYSQAPVPTSPQRARVAPQAKRLGGGFIAILGGI
                     AILAAQVLFISKQTTPLSLQNLLNVGLGLACLVSGIVSLAVRGWVPVILAALLSAAVF
                     ALCGLKLYQTVLWLQVQTPADATAWTLQSGGIIWVGGMMLGMVAFAKGFQTLFRR"
     gene            1904..3247
                     /gene="lysA"
                     /locus_tag="VN478_00805"
     CDS             1904..3247
                     /gene="lysA"
                     /locus_tag="VN478_00805"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HWQ21248.1"
                     /translation="MMRRLLRSQPERVRDLLVRRHTPGVVVERTRMNNDLMYQASGLM
                     LDRLPLEAVAEGYGTPVYVYSTASIRRQSTRLVQSLGTIGSLRYAVKANANPALLALI
                     FSMGIGADTVSQGEIRAALGAGCPADHIVFSGVGKTPDELAFALDRGVFINAECEEEL
                     TAIARLQAGTHVGIRVNPGVDAHTHQYMTTAMEENKFGIPIDAAPGAFRLAVNAGLVP
                     DTISCHIGSQIMSLEPYRKSLARLLELRDSLMSDGFPVTTLDIGGGFGISYDEGADFP
                     VEDLATLLRRDVPQGIKLWFEPGRYVVGHAGTLVTRVLYRKRYTRTFIVVDAAMNDLI
                     RPALYQARHAILPVTVRDSQPIPCDVVGPVCESADCFARDVPIPLPDQGDLLAICDTG
                     AYGWSMSSTYNSRSLLPEVLVDDGEPSLVRDRGGSETIPPAVEPGAGTVHWAGRS"
BASE COUNT          611 a         1051 c          998 g          609 t
ORIGIN      
        1 gctactgtcc gaagatcgac gccaggtcga ctttcgccgt catcatcgat atctggatgg
       61 aacgcggatc cacgtccatt cccttgatcg acgttcccgt aagaggggcg atccctcctt
      121 cgcggcgcgt cgacaactga cagtatccct ccttcaagag cgtggcttcc acgtcttcac
      181 tcagcaggta ctcggccaag ctgtctgcct catctggatg aggacctccc cggatgaccg
      241 caacgctcga cgggatcacc agagtcccca tcccatcctg gtctggcacg actactgaca
      301 ccgcgtcacc cctcttgact gccaccaaag catcatccga gtcggtgatc ccccatgtca
      361 acaccccctg tccaacgagg tcccggactg ttgcgttgcc gtcgactatc cgcacgccgg
      421 aggcgctcac ctgctggaag aaggcatagg cccgagccgc ccccagcacc gagtagagcg
      481 cggctgcgtg cgccgccgtc gtcccgaaga ggggcatggc catgccgacg ctggcacggg
      541 catacgctga cgacgccagg tccagcacag acgtggggga ggggcccttg aagaccttcg
      601 tattgagcag gagaacgcgg aaacgcgctg cgtggccggc ccacagtcca tccggatcgc
      661 ggtactgggc caggatcccc tggccggccc cttcgggaag ctggcgcagg acgttgttct
      721 gtaccagcgt cgacatttgc gtcgtctcgt tgttccagaa cacgtcgcac acagggtgcg
      781 cctgttccgc gacaagacgg ttgacgaggc cggtggtctt cgcggcctcc gtatcataga
      841 cgaccagcgc gcggacacca gtccgggcct cgaatgcctt gaacaagggg gccgcatact
      901 cttgatcgac tgacgtgtag cagacgacgg tggccccggg cgcccgacac ccggcgaaga
      961 gattggctgc caggataaca atgagcccga ccgccagtgg acgggcccgc aaatgattca
     1021 ccgccggaac agtgtctgga atcctttggc gaaggcgacc atgcccagca tcatgccccc
     1081 gacccatatg atgccgccgc tctgaagcgt ccatgctgtc gcgtcggcag gcgtctgtac
     1141 ctgaagccac agcaccgtct ggtacagctt caggccacac agggcaaaga cggcggcgct
     1201 caggagtgcc gcaagaataa cgggcaccca tcccctcact gcgagcgaga cgatcccgct
     1261 caccaggcat gcaagcccca gtccgacatt caacaggttc tgcaagctca agggtgtggt
     1321 ctgcttgctg atgaacagga cttgtgcggc caatatggcg ataccgccga ggatggcgat
     1381 gaatcctccg ccaaggcgct tggcctgtgg ggccacacga gcccgctgag gactggtcgg
     1441 cacgggagcc tgggagtaca cgcccgaacc aggtcctcca taggggctca ttggcgtcgc
     1501 accaggcccc ccataggatg cagcctgcgc aggcttcggt gagggtaccg gcggtgcgct
     1561 gtatggcccc gcactgacag gaggttgagg gagaggctcc tggacaggcg ttgtcggcgg
     1621 gaccgcctgc cggacgtcat ctgcaggagc gggatgctgg tcagcgacgt cggctccaca
     1681 attcggacag tgtttcgctt caggcggcag aggcttgcca cattcgatac agaacttcgc
     1741 ggattccatc gttgatcctc cagggcggtc tcgtattctc cgtacgtgtt cgacggccaa
     1801 gtgatcatgc attctgcgct cagtatatgg gggaggcggc gcgcttcaag gacgcctgca
     1861 gtatctggat cgccggccac ttgacaggga gttccgcgag cacatgatga gaagactgct
     1921 gcgatcgcag ccggaacgcg tgcgcgatct tctcgtccgg cgacacacgc ccggtgtggt
     1981 ggtggaaagg accaggatga acaacgacct catgtaccag gcttcgggtc tcatgcttga
     2041 tcgtctccct ctcgaggccg tcgcggaggg atatggcacg cctgtctacg tctattcgac
     2101 cgcgtcgatc cgcaggcaat ccacccgtct ggtgcagagt ctgggtacga tcggatcgct
     2161 tcgatacgcc gtcaaggcca acgcgaaccc tgctctcctc gcgctcatct tcagcatggg
     2221 tatcggagcc gacactgtga gtcaaggtga gatccgggct gctcttggcg ccggctgtcc
     2281 cgccgaccac atcgtcttct caggtgtcgg gaagacaccc gacgaactgg cgtttgcatt
     2341 ggatcggggg gtcttcatca atgccgagtg cgaggaggaa ctgactgcca ttgcccgctt
     2401 gcaagcgggt acccacgtgg gtatccgcgt caaccccggc gtcgatgcgc acacgcatca
     2461 gtacatgacg accgcgatgg aggagaacaa gtttggcatc ccgatcgacg ccgcccctgg
     2521 cgcattccgg ctagccgtga acgccgggct ggttcctgat acgatttcct gccacatcgg
     2581 cagtcagatc atgagcctgg agccctaccg caagtctctc gctcgcctgc tggaactccg
     2641 tgattccctg atgagcgacg gctttccagt gaccactctc gacatcggag gaggattcgg
     2701 catcagctat gacgaagggg cagacttccc agtggaggac ctggcaactc tcctgcgccg
     2761 ggacgtccct caggggatca agttgtggtt tgagcccggc aggtatgtcg tgggacatgc
     2821 gggcacgctg gtgacacgtg tcctgtaccg aaaacggtat accagaacgt tcatcgtcgt
     2881 ggacgccgcc atgaacgacc tcattcgccc ggccctgtac caggcgcgtc acgccatcct
     2941 tcccgtgact gtgagagatt cgcagcctat cccctgcgac gtcgtcggcc ccgtctgtga
     3001 gtccgcagac tgcttcgccc gcgatgtccc tatccctctg cccgaccagg gagatcttct
     3061 ggccatctgc gacacaggcg cgtacggatg gtccatgagc tcaacctaca acagtcgttc
     3121 cctgttgcca gaggtcctcg tcgacgacgg tgagccttcc ctcgttcgcg accgcggcgg
     3181 ttcggaaaca atacccccgg cagttgagcc gggggctggg acagttcact gggcggggcg
     3241 gtcgtgactg ccgtcccggg acgtacgcc
//
LOCUS       DATMZD010000038         2276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_21229,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000038 DATMZD010000000
VERSION     DATMZD010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21229"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..>2276
                     /locus_tag="VN478_00810"
     CDS             107..>2276
                     /locus_tag="VN478_00810"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HWQ21249.1"
                     /translation="MDAGEYVECDGTCLGDSLHEELQRLNDEAFALSDRDARAAKALA
                     DKMYAKATDAKSPYWQGLSRYIAGFCLHNLSEYDVAMEEFEHGDLIAQEHSLPALGIK
                     FRNGYGAIFSRLGRYHDAIEQYAEGLRMAREMGLKGDVGQYLINLGEVCLLMGDTAQA
                     LSFQSEAVELVSDLAVDARYATDVYYNLGEAQLKSGLVDDAERSYRRSLETATAIGNT
                     VCQVEASVRLSSILAGRGHEAEALTILEEAVELSRRCDFPLQEVASLIACGGIELSLG
                     HLEDATRHYGMAVEVADSHRMGDLLPSALESLSTTRAAMSCFEEAYHDLQRSVDAEHA
                     RSSSEAARMLAEIATGYRVEKVRREAELEKVHREALESANERLRTVARIGRSLTQSVD
                     PRDILMRMWKELSTSIDLKWLGFGIYSPDSGEIEFPGLIDSGVLQKASTVFVSDESSL
                     AALCVREKRTLYYATPGEARSALGEHALIMFGSLASAPQTILYLPLFIDNEIVGVMTV
                     QSATDHAYSDEVIEMLEAVASFTAVAVENARIMIRLNEMNRIISGEKEQVEKAALASS
                     WLAEHDSLTGLYNRRILEKVLDEDIRLATLEGDHIAVFFIDLDDFKGVNDARGHDAGD
                     RVLVAVADRLLSVFRQGDYIARVGGDEFIVVAPGVKSVDSATSMADGVVASFAEPLSV
                     AEGFLPVTISVGLAVFPEQGSSSRELIGRADEAMYSGKRTG"
BASE COUNT          454 a          656 c          748 g          418 t
ORIGIN      
        1 atgaatatct gagcccttcc tgttcgttcg gcgagtgcag cgcctgcgtt ccatcacgga
       61 gtgatgtaca aaacaacagc agagatatac taatgaaacg atggaaatgg acgcgggcga
      121 gtacgtggaa tgcgatggaa cgtgtctcgg ggactccctt cacgaggaac tgcagcggct
      181 gaatgacgag gcctttgctc tctctgacag ggacgcgcgg gccgcgaaag cgctggctga
      241 caagatgtat gcaaaagcca cggacgcgaa gagtccgtac tggcagggac tgtcccgata
      301 tatcgcaggc ttctgtcttc acaacctcag cgagtacgat gtcgccatgg aggaattcga
      361 gcacggcgat ctgatcgccc aggaacacag ccttccggcc cttgggatca agttccgcaa
      421 cggatacggg gccatcttct cccggctggg acgctaccat gacgcgatcg agcagtacgc
      481 ggaaggactg aggatggcca gggagatggg cctgaagggc gacgtcggcc agtacctcat
      541 caacctcggg gaggtctgcc tcctcatggg cgataccgcc caggcgctct cctttcagtc
      601 ggaggctgtc gaactcgtgt ccgacctggc cgtcgacgcc aggtatgcga ccgacgtgta
      661 ctacaatctg ggcgaagcgc agttgaagag tgggctcgtg gacgacgcgg aacggtccta
      721 ccgtcggagt ctggagaccg ccacggcgat agggaacacg gtctgtcagg tggaggcgag
      781 cgttcgtctg agctccattc tcgccggtcg cggccatgaa gccgaggcgc tcaccatcct
      841 cgaagaggcg gtcgaactga gccgtcgttg tgacttcccc cttcaggagg tcgcatcact
      901 cattgcctgt ggaggcatcg agctcagcct cggtcatctt gaggacgcga cccgtcacta
      961 cgggatggca gtcgaggtag ctgacagcca ccgcatggga gaccttctgc catcggccct
     1021 ggagtcgctc tcgacgaccc gggccgccat gagttgcttc gaggaagcat atcacgacct
     1081 ccagagatcc gtcgatgcgg agcatgcgcg gtcgagctca gaggccgcca ggatgctggc
     1141 cgagatcgcg acaggttatc gagttgagaa ggtgaggcgg gaagctgagc tcgagaaggt
     1201 gcaccgcgag gcactcgaat ccgcaaacga gcgtctccgg accgtggccc gtatcggacg
     1261 gagcctcacg cagagcgttg atcccaggga catcctcatg cggatgtgga aggaactatc
     1321 gacgtcgatc gacctcaagt ggctgggatt cggcatctac agccccgatt ccggcgagat
     1381 cgagtttcct ggattgatcg actccggagt cctgcagaag gcatcgacgg tctttgtgag
     1441 cgacgagtcg agtcttgctg cgctctgtgt gcgggagaag cgcaccctct actacgcgac
     1501 gccgggcgaa gcgcggtcgg cgcttggaga gcatgcgctc atcatgttcg ggtccctggc
     1561 gtctgcgcca cagacgatcc tgtatcttcc actgttcata gacaatgaga tcgtcggcgt
     1621 catgacggtg cagagtgcca cagaccacgc ctactccgac gaggtcatcg agatgctgga
     1681 ggcggtggcc tcgttcacgg cggttgccgt cgagaatgcg cgcatcatga tccgactgaa
     1741 cgagatgaac cggatcatct cgggcgagaa ggagcaggtc gagaaggccg cccttgcgtc
     1801 ttcctggctg gcggagcacg acagcctgac cgggctctac aacaggcgca ttctcgagaa
     1861 ggtgctcgat gaggacattc ggctggcgac ccttgagggc gaccacatcg ccgtcttctt
     1921 catcgacctc gacgacttca agggcgtgaa cgacgcgcgt gggcacgacg ccggcgatcg
     1981 cgtcctcgta gctgtcgcgg accgcctgct ctctgtcttc cgccaaggag actacattgc
     2041 gcgggtgggg ggagacgagt tcatcgtggt ggcaccgggc gtcaagagcg tggacagcgc
     2101 gacgtcgatg gccgacggag tggtcgcatc atttgctgag ccactgtcgg tcgctgaagg
     2161 ctttttgccc gtcaccatat cggtcggcct cgcggtgttc ccggagcaag gcagcagcag
     2221 ccgcgagctg atcggccgcg cggacgaggc gatgtactcg ggaaagcgga caggga
//
LOCUS       DATMZD010000039         5211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_81744,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000039 DATMZD010000000
VERSION     DATMZD010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5211
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81744"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="VN478_00815"
     CDS             complement(<1..710)
                     /locus_tag="VN478_00815"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HWQ21250.1"
                     /translation="MNRTEETGVVLELQRLSTEDGPGVRTTVFLKGCSLTCAWCHNPE
                     SIDPRPQVQWMGNRCIGCRTCVTACPQHALDLTGTGMQLDRSLCTGCGVCVTTCPSGA
                     MDLLGARWTAQGLLAEVVKDRAYFGPDGGVTVSGGEPALQGEFCRAFLAAARAAGLHT
                     AVDTCGMAATDRFLAVAREADLVLYDLKEADGVRHKTFTGQDNTIILANVLLLADMMR
                     HEGRPRTLWIRTPLIPGAT"
     gene            complement(720..3239)
                     /locus_tag="VN478_00820"
     CDS             complement(720..3239)
                     /locus_tag="VN478_00820"
                     /inference="COORDINATES: protein motif:HMM:NF014900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HWQ21251.1"
                     /translation="MAQTERMVASFHVKEPKGLSPRIQWLRDYYFRGVSRPWNNEYTC
                     WSTGTPWDVQYDEITYYIVPETYAFLQTFRSSVRQASRPVPLEPRFWSWSLPERRAWF
                     NREVMVNHVPHEVLPGDLLAGARFNVQTSRCWSEKELKERDALVYGPSGARASMKWFH
                     DHGYGNAGATSGHLIPDYATVLRIGWKGVHGKLEQAYDALGDADRKGPKGAQLRAMIT
                     ASTMARDVAAKYHDELLRLAGEEQDDVRREELLAMTGNLERVPWKPARTFWEAVQSLW
                     LTHMLVMSDENYPGPGVSFGLLDRMLLPYWQASLAEGMDREWGKEILKCFWVHCNYAY
                     DAQVRVGANQGITAGFGQLFNLSGIGPDGKDATNDLTYAMLEVLDDMSPILEPKPNVR
                     LHRHTPEKLLDKVVDMIAGSQGAPFLLNFDERSMAGMMREANNGHVEKLIDRSNVNTY
                     ASVGCLENTMVGNDRSGTVDCNLNLLKAVELTLTGGRDLLATTDGMTGKVLQPRQDSV
                     ATGDPANFRTWDDFWQAYVAQTKAVVKRITELYEKSETIRARYSPTPYLSCLVRGCME
                     KGLDVTQGGAELSFVTVEGVTFATTVDSLLAIKDLVYDARVVTMDELMTALRDNWKGH
                     EALQARAKNKTPKYGRDDETADAMARAVMDLWTAETWKYRTKSTARQYRPGMLSWNYW
                     VGDGYVMAASADGRPQGQFLSNAICPSNGADVKGPTANANSVGTALGGKAEEGDGDFE
                     GYLNCLPNGASHTITFSPSILRDPEHRAKFKAFLRGYVENGGTALQINMLDAGMLRDA
                     QAHPGNYRNLLVRVTGYNAYFASIGRELQNEIIARESHNAY"
     gene            complement(3226..4047)
                     /locus_tag="VN478_00825"
     CDS             complement(3226..4047)
                     /locus_tag="VN478_00825"
                     /inference="COORDINATES: protein motif:HMM:NF019456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HWQ21252.1"
                     /translation="MTTNNAYQEWIGSRDTAGERKPMKKPVEPPVAVSPKREFIGCFE
                     GLILSGTIAMGERLPPERSIAAQLGVSRPVVHEGLLELASRGLVTIRPRHGAVVNDYR
                     TQGSVTMLASLVAYQRGELEPSLAAGLIDMRRLFETETARRAALNRTDEQIGSLRLLV
                     EREKAAAEGDIAEVTRLDFQVHHEIALASGNPVYAMLMKSFEPAYTNLSGRFFAVPGM
                     ARGVVKGHEALVQLIEQRDAEGAVSVMRSLLEQGAKVLLGLQHAADAGRKSNGTD"
     gene            4173..4745
                     /locus_tag="VN478_00830"
     CDS             4173..4745
                     /locus_tag="VN478_00830"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HWQ21253.1"
                     /translation="MDQNRKRLRRGGAVAVAVSGDAADYVDSFRRLYDPHVASILPHV
                     TLAVAPELDVGDWMSARPLIKEALSQVQPFAIRVAELGTFADDLVLWLRPTVPHGELV
                     TMRKMILGAFPDIEFDRVHDFVPHISIGFFTGRQALLLAMDAIRPELVPFSFQVAYVS
                     FLQADEGDVWQCVDTVELGGLNTGITCPKD"
BASE COUNT          876 a         1695 c         1607 g         1033 t
ORIGIN      
        1 gtggcgcccg ggatgagcgg cgtccggatc cacagtgtcc ggggacgtcc ctcgtgccgc
       61 atcatgtccg cgaggaggag cacgttcgcc aggatgatcg tgttgtcctg accggtgaag
      121 gtcttgtggc ggacgccgtc agcttccttc aggtcgtaga ggaccaggtc cgcctcccgc
      181 gccacggcaa ggaaccggtc cgttgccgcc atgccgcatg tgtcgacggc cgtatgcagg
      241 ccggccgcgc gcgccgcggc gaggaacgcc cgacagaatt ccccctggag cgcgggctcg
      301 ccacccgaga ccgttacccc tccgtctgga ccaaagtagg cgcggtcctt gacgacttcc
      361 gccagcagac cctgcgcggt ccacctggcg cccagcaggt ccatggctcc cgacgggcat
      421 gtggtgacac agacgccaca gccggtgcaa aggctcctgt cgagctgcat gcccgtgccg
      481 gtcagatcaa gagcatgttg cggacaggcg gtgacacagg tgcgacagcc gatgcagcga
      541 ttccccatcc actgcacctg cggcctcgga tcgatgctct ccgggttgtg gcaccacgcg
      601 catgtcagtg aacatccctt gagaaacacg gtcgtccgga cgccgggccc gtcctccgtc
      661 gacagccgct ggagttcgag gacgacgccg gtttcctctg tcctgttcac caccagcccc
      721 tagtacgcgt tgtgactctc acgtgcgatg atctcgttct gcagttcgcg cccgatgctt
      781 gcgaagtagg cgttgtagcc cgtcacacgg accagcaggt tgcggtagtt gcccggatgc
      841 gcctgggcgt cgcgcagcat gccagcgtcc agcatgttga tctgcagggc cgtaccgcca
      901 ttctcgacgt acccgcgcag gaacgccttg aacttggctc gatgctcggg gtcgcgcagt
      961 atcgacgggc tgaacgtgat ggtatggctc gcgccgttgg gcagacagtt gaggtagccc
     1021 tcgaagtcgc cgtccccctc ctccgccttg cctcccaggg cggtcccgac cgagttggcg
     1081 ttggccgtgg ggcccttgac gtcggcaccg ttgctcggac agatggcatt gctgagaaac
     1141 tgtccctggg gacgaccgtc ggcactggcc gccatgacgt agccgtcgcc cacccagtag
     1201 ttccagctga gcatgccggg gcggtactgc cgcgccgtgc tcttcgtccg gtacttccac
     1261 gtctcggccg tccagaggtc catgacggcg cgcgccatgg cgtcggctgt ctcgtcgtcc
     1321 cgaccgtatt tgggtgtctt gttctttgcc cgtgcctgca gggcttcgtg tcccttccag
     1381 ttgtctctca gggcggtcat cagttcatcc atcgtgacga ccctcgcgtc gtagacaagg
     1441 tccttgatcg ccagcagcga gtccacggtc gtggcgaatg tgacgccctc caccgtgaca
     1501 aagctcagct cagccccgcc ctgtgtgacg tccaggcctt tctccatgca gccccgcacc
     1561 aggcaggaca ggtagggggt cggcgagtac cgcgctcgaa tggtctccga cttctcgtac
     1621 agctcggtga tacgcttgac aacggccttt gtctgcgcga catacgcctg ccagaagtcg
     1681 tcccacgtcc ggaagttcgc agggtcgccc gtggcgacgc tgtcctgccg cggctggagc
     1741 accttgcctg tcatcccgtc tgtggtggca agcaggtccc tgcctcctgt cagggtcagc
     1801 tcgacagcct tgagcagatt gaggttgcaa tccaccgtcc cactgcggtc gttgccgacc
     1861 atcgtgttct cgaggcaacc cactgaggca taagtgttga cgttgctcct gtcgatcagc
     1921 ttctcgacgt gtccgttgtt tgcctcgcgc atcatgccgg ccatggaacg ctcgtcgaaa
     1981 ttaagcagga acggggctcc ctggctgccc gcgatcatat caaccacctt gtccagcagc
     2041 ttctcgggcg tgtgccggtg caggcggacg ttgggcttcg gctcgaggat ggggctcatg
     2101 tcgtcgagca cttccagcat cgcgtaggtg aggtcgttgg tcgcgtcctt gccgtcgggt
     2161 ccgatgccgg agagattgaa cagctgcccg aagccggccg tgatgccctg gttggcgccg
     2221 acgcgtacct gggcgtcata ggcatagttg cagtggaccc agaagcactt gaggatctcc
     2281 ttgccccact cgcggtccat gccctccgcc agggatgcct gccagtaggg caggagcatg
     2341 cggtccagga gcccgaatga gaccccgggt cccgggtagt tctcgtcgct catgaccagc
     2401 atatgtgtca gccacagtga ctgcacggcc tcccagaatg tgcgcgccgg cttccagggg
     2461 acgcgctcca ggttgccggt catcgccagc agctcttctc tgcgcacgtc gtcctgttct
     2521 tcccccgcca gacggagcag ctcatcatgg tactttgcag ccacgtcgcg cgccatcgtg
     2581 ctggccgtga tcatcgcgcg cagctgtgcg cctttcggtc ccttcctgtc tgcgtctccg
     2641 agggcatcgt acgcctgttc cagcttgcca tggacgccct tccagccgat gcgcaggacc
     2701 gtggcgtagt cagggatgag gtgacccgac gttgctccgg cgttgccata cccatgatca
     2761 tggaaccact tcatggacgc cctggcgccg gagggtccat agaccagcgc gtcgcgctcc
     2821 ttgagttcct tctcggacca gcagcggctg gtctggacgt tgaaccgtgc gcccgccagc
     2881 aggtcgccgg gcaggacctc gtggggcacg tggttcacca tgacctcacg gttgaaccat
     2941 gcgcgcctct ccggcaggga ccatgaccag aacctcggtt cgagcggaac ggggcgggac
     3001 gcctggcgca cggagctgcg gaaggtctgc aggaacgcat aggtctcagg tacgatgtag
     3061 taggtgatct cgtcgtactg gacgtcccat ggtgtcccgg tgctccagca cgtgtattcg
     3121 ttgttccatg ggcgcgagac tcccctgaag taatagtccc tcagccactg gatacgcggt
     3181 gagagccctt tcggctcctt gacgtggaat gacgcaacca tccgctcagt ctgtgccatt
     3241 gctcttcctc ccggcatctg ccgcatgctg cagccccagc aggacctttg cgccctgctc
     3301 gagcaaggat ctcatgacgc tgacagcgcc ttccgcgtcg cgctgttcga tcagctggac
     3361 caacgcctcg tgccccttga cgacgcccct cgccatcccg gggacggcga agaacctgcc
     3421 ggacaggttc gtatacgctg gctcgaacga cttcatgagc atggcgtaga caggattccc
     3481 ggacgccaga gcgatctcgt gatggacctg gaagtccagc ctggtcacct cagctatgtc
     3541 accctccgcc gcagccttct ccctctcgac gaggagcctc agtgagccga tctgttcgtc
     3601 ggtccggttc agggcggcgc gccgtgccgt ctccgtctcg aacagccgac gcatgtcgat
     3661 gagtccagcg gccagtgaag gctcgagttc tccccgctga taggcgacga gggacgcgag
     3721 catggtaacc gacccctgcg tcctgtagtc gttgacgacc gctccatggc ggggcctgat
     3781 cgtgaccagg ccgcgggagg cgagctccag gagtccttca tggacgaccg gccgacttac
     3841 gcccagctgt gcagcgatcg accgctccgg cggcaggcgc tcacccatgg cgatcgttcc
     3901 tgagaggatc agcccctcga agcagccgat gaactctctc ttgggagata ctgcaactgg
     3961 tggttccacg ggtttcttca tcggcttcct ttcaccggcc gtgtcacgtg aaccgatcca
     4021 ctcttggtac gcattgttgg tggtcatacc acctgacatc ctcacgatag gcggaggccc
     4081 cggattgtca agggtcgtgg catacggaga cagaggctcg acggtcgcaa atcctgcgtt
     4141 gcgagctggt tcaatgctcg tatactggtg tcatggacca gaacagaaaa aggcttcgca
     4201 ggggtggggc tgtcgcagtc gccgtatcag gcgacgccgc agactacgta gacagcttcc
     4261 gcagactgta cgacccccat gtggccagca tattgccgca tgtcacgctc gccgtcgcac
     4321 cagagctcga cgtcggagac tggatgtctg cgcgtcccct gataaaagag gcattgtctc
     4381 aagtccagcc gttcgccatc cgcgtcgccg agctcgggac tttcgcggac gacctcgtac
     4441 tgtggctgcg accaactgtg cctcacggcg aactggtcac gatgcgaaag atgatcctgg
     4501 gggcatttcc ggatatcgag ttcgaccgcg tacacgactt cgtgccccac atctccatcg
     4561 ggttcttcac tggtcgtcag gcactgcttc tggctatgga cgccatacgt cccgagctgg
     4621 tcccgttctc attccaggtg gcctatgtct catttctcca ggcagatgaa ggcgacgtct
     4681 ggcaatgtgt cgacactgtg gaactggggg gattgaacac cggaatcacc tgccccaaag
     4741 actgagggcc aggacacgtc agaaatagcg atcatcaagg ggggaaccat gagcatgttc
     4801 tactgcgata cttgtcacaa gaagttcgag gctgagggcg tcaagaccga ctggatcgac
     4861 cgaacgtacg gcccttgtac gagatggacc gcctcgtgcc ccgactgcgg cgaggaatgc
     4921 cggctggcga agaccgtcca cacacatggc gacggtgagg acggctgcga aggtggctcc
     4981 tgtggctgtg gaggcggttc gtgcagctgc gggggctccg gatgctcctc ctgtggagga
     5041 tgctgacaag cctccaggcg gtcatcccgg ggctgcgaaa attcggtcct tgaacgtcca
     5101 tgagacgctt ctcgccggat cagactgacc gacgagtgta ccacccggcg cgtaattgcc
     5161 agctgacccc acggccgctg accccataca agccgccacg gcccgcatca c
//
LOCUS       DATMZD010000040         2896 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_31424,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000040 DATMZD010000000
VERSION     DATMZD010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31424"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1876
                     /locus_tag="VN478_00835"
     CDS             <1..1876
                     /locus_tag="VN478_00835"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HWQ21254.1"
                     /translation="CHVMAHESFEDAETAGIMNAQFVCIKVDREERPDLDSVYMSAVG
                     ALTGHGGWPLSVFLTPDLQPFYGGTYFPPEPRHGLPGFPQLLRAVADAWRDRRPEIAR
                     AGAQVTAQLVNQSRATAPEGAFGIGTLTSATHSLIGQYDWKNGGWGTAPKFPQPMAIE
                     FLLRRHLASDTEALAPAVHALRAMARGGMWDVIGGGFARYSTDDTWHVPHFEKMLYDN
                     ALLARAYLHAWQVTGEASFRRVVDQALTFVAREMTTPEGAFLSSLDADSEGVEGRYYV
                     WSLDELRTVLGENTSLFAEAYGVTEGGTWEGTNVLQRSADDATLAARHGMSPDDISEA
                     LERSRARLFVARGDRVRPAADDTVLTAWNGLMLSSFAEAARVFANQAYLDAATRNANF
                     LLSALRPGGHLQRTWRNGVAGQPAYLDDYASLILGLLDLYQTDFDNRWFAAAKVLAEE
                     MLTRFTDPAGGFFDTPDSAEQLMLRPKDLQDNAVPSGSALAVEALLTLASLTGSREWR
                     RLAGRALELVSDLVMSYPTAFGRWLCAADLALGDEIEVAIVGDPVDRRTRALVAEIRA
                     AWRPSLVVALSSAPLPSGAPDLLADRPMVAGQPTAYVCEGSTCRQPVTTPDDLRVQLR
                     "
     gene            1994..2518
                     /locus_tag="VN478_00840"
     CDS             1994..2518
                     /locus_tag="VN478_00840"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWQ21255.1"
                     /translation="MSAQVIVITGPRASGKSAFAGEVHAALPGSILLHVDEPFHRILD
                     ENRDQWTGKEIELYDSVLRRQLSLARGAACAGGCVIVDGTVLPDQLEPLRASPASDVT
                     VIVLLPTLEECLANEHRSTRTVPVREEKVTATWRQMQEWRHVDQDFVHILEVPQEDRG
                     DWLAALLLRMDGKL"
     gene            complement(2515..>2896)
                     /locus_tag="VN478_00845"
     CDS             complement(2515..>2896)
                     /locus_tag="VN478_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HWQ21256.1"
                     /translation="AVFGLRWKLNVLSMGDDEARSLGENPIVMRFVFVGLTTMMTAVS
                     VALSGVIGWVGLVVPHVARGIVGPDSRRLIPASIVLGAAYMIIIDGVARSLTTAEIPL
                     GILTGIIGAPAFVLLLRRSRSDWS"
BASE COUNT          517 a          915 c          940 g          524 t
ORIGIN      
        1 gtgtcacgtc atggcacatg agtcctttga agacgcggag actgccggca tcatgaacgc
       61 ccagtttgtc tgcatcaagg tggaccgtga ggagcgacct gatcttgaca gcgtctacat
      121 gtctgcggtc ggggcgctga cgggtcatgg cggctggccg ctgtcggtct tcctgacacc
      181 ggacctgcag ccgttctacg gtggtaccta cttccctcct gagccgcgtc acggcttgcc
      241 tggctttcct caactcctgc gtgcggtggc tgacgcttgg agagaccgac ggcccgaaat
      301 tgcgcgtgcc ggggctcaag tcactgcgca gctcgtcaat cagtccaggg caaccgcccc
      361 ggaaggggcc tttggcatcg gtacactgac cagtgccaca cactccctga ttgggcagta
      421 tgactggaag aacggcggct ggggcacggc ccccaagttt ccccagccga tggctatcga
      481 attcctgctg cgacgccatc tcgccagtga cacagaagcg cttgcgccag ccgtgcatgc
      541 cctgcgggcg atggcaaggg gcggtatgtg ggatgtgatc ggcggagggt ttgcgcgcta
      601 ctcgacagac gacacgtggc acgtcccgca ctttgagaag atgctgtacg acaacgcact
      661 gctcgcacgt gcctacctcc acgcttggca ggtgaccggc gaggcctcgt tccgccgtgt
      721 tgtcgatcag gccctgacgt tcgtcgcccg ggaaatgacg acccctgaag gcgccttcct
      781 gtcaagcctc gacgccgact cggagggtgt ggagggtcgc tactacgtct ggagccttga
      841 cgagctccgc acggtgcttg gggagaatac cagcctgttc gccgaagcat atggtgtgac
      901 tgaaggcggc acgtgggagg ggacaaacgt cctgcagcgt tccgcggacg acgcgacgct
      961 tgccgcccgc catggcatgt ccccagacga tatcagcgag gccctcgaac ggtcgcgtgc
     1021 cagacttttc gtcgcacggg gcgatcgggt gcggccggcc gccgacgaca cagtgttgac
     1081 cgcatggaat ggtctcatgc tttcttcgtt cgccgaagcg gcgcgcgttt tcgcgaacca
     1141 ggcgtacctt gacgccgcga cccgcaacgc caacttcctg ctcagcgcat tgcgacccgg
     1201 cgggcacctg cagcgcacat ggcgcaatgg tgttgctgga cagccggcgt acctggacga
     1261 ctatgcttca ctgatccttg gactgctgga cttgtaccag acggatttcg acaaccgctg
     1321 gtttgctgca gccaaggtcc tcgccgagga gatgctgacc cgcttcaccg atcctgccgg
     1381 agggttcttc gacacgccgg acagcgcgga gcagctgatg ctccgtccca aggacctgca
     1441 ggacaatgcc gtcccgtcgg gaagtgccct ggccgtggag gcattgctga cacttgcttc
     1501 tctcactggc tccagggaat ggcggagatt ggcaggacga gctcttgaac tcgtgagcga
     1561 ccttgtcatg agctacccga ctgccttcgg tcgctggcta tgcgctgcgg atctcgccct
     1621 gggggatgag atagaagtcg ccatagtagg ggacccggtt gaccggcgaa ccagggccct
     1681 ggtcgctgaa atccgcgcag cgtggaggcc cagcctggtc gtggcattgt catcggcgcc
     1741 actaccctca ggagcccccg acctgctcgc tgaccgtccg atggtagccg gtcagcccac
     1801 cgcctacgtc tgcgaagggt ccacctgccg ccagccggtg acgacgcccg acgatctgcg
     1861 ggtacaactg agatgacgcg cagccggtga cggtcctgac cccgattgtg aatgccgtaa
     1921 gtacctggta ctatgggatt cacaattcgg ggagtggcgt tgccaggcaa ccttcacgtc
     1981 gtagaatgat ggcatgagcg cacaggtcat cgtgatcacg ggtcccaggg caagtggaaa
     2041 gtccgccttc gcaggcgagg tccatgcagc actgcctgga tcgatcctcc tgcacgtcga
     2101 cgagccgttt caccggatcc tggacgagaa tcgcgaccag tggacgggca aggagatcga
     2161 gttgtatgac agcgtgctgc ggcgccagct gagtcttgcg cgtggcgccg cttgcgccgg
     2221 cggctgcgtc atcgtggatg ggactgttct cccggaccag cttgagcctc tccgcgcgtc
     2281 tcccgcttcg gacgtcaccg tcatcgtcct cctgcccacg ctcgaggagt gtctggcgaa
     2341 tgagcatcgg tcaacacgca cggtgccggt ccgcgaggag aaggtgacgg cgacatggcg
     2401 ccagatgcag gagtggaggc acgtcgatca ggacttcgtc cacatcctgg aagtcccgca
     2461 ggaggatcgc ggagactggc tggctgcgct gctgctccgg atggacggga agctctagct
     2521 ccagtcggac cggctgcggc ggagaagcag cacgaaggcg ggggcgccga tgatgccggt
     2581 caggatccca agcgggatct cggccgtcgt caggctgcgc gcgacgccat cgatgatgat
     2641 catgtaggcc gcccccagga cgatcgaggc agggatgagc ctgcgcgaat ccggccccac
     2701 gatcccgcgc gcgacgtgtg gcacgaccag gcccacccac ccgatgactc cggagagtgc
     2761 gacactgacg gccgtcatca tcgtggtcag gccgacgaaa acgaacctca tcacaatggg
     2821 attctcgccc agcgaccgcg cctcgtcgtc gcccatcgag aggacgttca gcttccagcg
     2881 gagaccgaac acggcc
//
LOCUS       DATMZD010000041         3194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_1500,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000041 DATMZD010000000
VERSION     DATMZD010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1500"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VN478_00850"
     CDS             complement(<1..236)
                     /locus_tag="VN478_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3' terminal RNA ribose 2'-O-methyltransferase
                     Hen1"
                     /protein_id="HWQ21257.1"
                     /translation="MLLTITYTGRNTPDLGYLLHKNPYRPQTLELNYGRAHVFYPEVS
                     DERTTVALLLDIDPIDLARGKTGLTSGGLFDYVN"
     gene            complement(605..2149)
                     /gene="guaA"
                     /locus_tag="VN478_00855"
     CDS             complement(605..2149)
                     /gene="guaA"
                     /locus_tag="VN478_00855"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HWQ21258.1"
                     /translation="MNAAAKHELVLILDFGGQYTQLIARRVRELHVFSEIIPFDTPLE
                     TLTLRNPKAIILSGGPASVYAASAPVCDTSVLRAGIPVLGICYGAQLMAMLLGGSVEH
                     APAGEYGKTTVQLDGTACLFGGLPTETPCWMSHTDRIGMIPPGFRATASTAACPVAAM
                     ENPEQHQYGIQFHPEVVHTPVGRDVLRHFLFDAAGCSGDWTMSAFADDAIEDIRSRIG
                     DERALCALSGGVDSSVAATLVHRAIGDRLTCVFVDTGLLRAGEGDRVESVFHRRFDMP
                     LVRIDASARFFASLKGVTDPEKKRRAIGAAFIDCFAEQARAMQGIPFLVQGTLYPDVI
                     ESGSSVASTIKSHHNVGGLPADLSFALVEPLRLLFKDEVRDLGRALGLPEELVDRQPF
                     PGPGLGVRILGEVTPEGVDILQRADAVVERVMRAAGWYTKVWQSFAVLPGIRTVGVKG
                     DERTYVHTIALRVVESSDGMTADWVRLPPDVLEDMAHAITSELPEVNRIVYDITSKPP
                     STIEWE"
     gene            complement(2146..>3194)
                     /locus_tag="VN478_00860"
     CDS             complement(2146..>3194)
                     /locus_tag="VN478_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012957027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HWQ21259.1"
                     /translation="SSDLAFEEPSTLLSQLLHTSSKIAVPATLDAQGLAAYLAKWGMR
                     ELALTNMEGQLAGFATIDEITRTLASPEVVMDEGGRLLVGAAVGVSTDLLARARSLVE
                     AGTDLLVLDSAHGHSLGIVRAVALLKQAFPVVPILAGNVATPEAVRDLVIAGADAVKV
                     GIGPGSICTTRIIAGVGMPQITAIYECALEAQRHDVPIVADGGVRYSGDVTKALAAGG
                     STVMLGGLLAATVESPGEVVTVGSKQYKSYRGMGSLGAMEQGSSDRYFQDTRLKLVPE
                     GIEGRVPLRGSVHDVLYQLLGGLRAGMGYCGTPTLDLLRSESRFVRITGAGLRESHPH
                     DVDMTGESPNYSPE"
BASE COUNT          614 a          973 c         1004 g          603 t
ORIGIN      
        1 tcattgacgt agtcaaacag tccaccgctc gtcaggcccg tcttgccgcg ggcaaggtca
       61 atgggatcga tgtccaatag cagcgccaca gtcgtgcgct catccgacac ttccggatag
      121 aagacatggg ctctgccata attgagttcc agcgtctgag gacggtatgg gttcttgtga
      181 agcagatacc cgaggtcagg cgtattccgt cccgtgtacg ttattgtcaa gagcatgttt
      241 ttgcccccct gcagacgttc ttgctcctct tggaggatct gtcctcaagt tcgccgtagc
      301 cagattgctt tgcctcgaaa acttccgaga catcatattc gtgcgccgtg ggttttcaag
      361 caacgactca ggcgatgcaa gtgtatgcgt gaaccacagc cgatcggacg cttttcgacg
      421 gtttgctatt ccgtatcgac agtctggagt ctctgactgg ggcaacagga gacaggagct
      481 acttcatcag cacagagatg tctcgccaaa ggccgcaagc acggtcagcc atagtggact
      541 gtcagtggct gaggggaagc tccagaaggc tggaccttcc gaagcgccct gtgctgtggt
      601 tcctctattc ccactcgatc gtggaggggg gtttcgatgt gatgtcgtag acgatccggt
      661 tgacctcagg cagttcgctg gtgatcgcgt gcgccatgtc ctcgagcaca tcaggaggaa
      721 gccgtaccca gtcggccgtc atgccgtcgc tgctctcaac cacgcgcagg gcgatcgtgt
      781 gaacgtacgt acgttcgtca cccttgacac caacggtccg gatgccggga aggacggcga
      841 atgactgcca cacctttgtg taccatcccg ccgctcgcat tacgcgttcg acgacggcgt
      901 ccgcgcgctg gaggatatcg acaccctcgg gtgtcacctc gccgaggatc cgaacaccta
      961 ggccgggacc agggaacggc tgtctgtcca ccagttcctc gggcaggccc agagcgcggc
     1021 cgaggtcgcg cacctcgtcc ttgaacagca gccgcagcgg ctcgacgaga gcaaacgaga
     1081 gatctgccgg caggccgccg acattgtgat ggctcttgat ggtgctggcg acggagcttc
     1141 cggattcgat gacgtccggg tacagtgtcc cctggacgag gaaagggatg ccctgcatcg
     1201 cacgcgcctg ctcggcaaag cagtcgatga aggcggcacc tatggctctt cgcttcttct
     1261 cgggatcggt gacgcccttc agagaggcga agaatcgagc ggaagcgtcg atgcgcacaa
     1321 ggggcatgtc aaaccggcgg tggaacactg actcgacacg gtcgccctct ccggctctga
     1381 gcagccctgt gtcgacaaag acgcacgtga ggcgatcgcc gatggcgcgg tggaccagtg
     1441 tcgccgcgac cgaactgtcg acgcctcccg agagggcgca gagcgcgcgc tcatcgccga
     1501 ttcgcgaacg gatatcctcg atggcatcgt ccgcgaacgc agacattgtc cagtcgccgg
     1561 agcaacccgc agcgtcgaag aggaagtggc gcaggacgtc gcgcccgacg ggcgtatgaa
     1621 ccacctcggg gtggaactgt atgccatact ggtgctgctc aggattctcc atggcggcaa
     1681 cgggacaagc ggcggtcgat gcggtagccc ggaagcccgg ggggatcatg ccgatgcgat
     1741 cagtgtgact catccagcag ggggtttctg tcggaaggcc gccgaacagg caagcagtcc
     1801 cgtcgagctg gacggtcgtc ttgccatatt cgcccgccgg agcgtgttcc acggagcctc
     1861 ccagcagcat agccatcagc tgggctccgt agcagatgcc gagaactggt atcccggccc
     1921 tgagaacgga cgtgtcacag acgggggcgg aagctgcata gacgctcgca ggaccccccg
     1981 aaaggatgat cgccttcggg tttctcaggg tcagcgtctc gagcggcgtg tcgaagggga
     2041 tgatctcgct gaacacgtgg agttcgcgga cccggcgtgc gatgagctgg gtgtactgac
     2101 cgccgaagtc caggatcagg acaagttcgt gcttggcggc agcgttcatt ccgggctgta
     2161 gttcgggctt tcgcccgtca tgtcgacgtc atggggatgg gactcgcgaa ggccggctcc
     2221 cgtgatccgg acgaagcggg actcactgcg gaggagatca agggtcggcg tgccgcagta
     2281 gcccatgccg gctcgcagac caccaagcag ctgatacagg acatcgtgga ctgaccctct
     2341 cagaggaaca cgaccctcga tgccctcggg caccagcttg agccgggtat cctggaagta
     2401 cctgtcactg cttccctgtt ccattgcgcc aagggatccc atgccccgat agctcttgta
     2461 ctgtttgctc cccaccgtga cgacctcgcc cgggctctcc actgtggcgg caagaaggcc
     2521 gccgagcatg actgtgctgc ctcctgctgc aagagccttg gtgacgtccc cggagtaccg
     2581 gacgcccccg tcggcaacga tgggaacgtc atgacgctgc gcttccaggg cacactcgta
     2641 gatggctgtt atctggggca tcccgacccc tgctatgatg cgtgtcgtgc agatggagcc
     2701 cggccctatg ccgaccttca ctgcgtcagc tcccgcgata accaggtcgc ggactgcctc
     2761 cggtgtcgcc acgttgcccg ccaggatggg aacgacggga aacgcctgct tgaggagtgc
     2821 gactgctctg acgatgccaa gggaatggcc atgggcactg tccagcacga gcaggtctgt
     2881 cccagcctcg acgagcgagc gagctcgcgc gagcaggtcg gtggaaacgc cgacggcggc
     2941 tccgacaagc agcctgcctc cttcatccat cacgacctcg ggagatgcca gcgtccgcgt
     3001 tatctcgtcg attgtcgcaa aacctgccaa ctgcccttcc atgttggtca aggccagttc
     3061 ccgcattccc cacttggcaa gatacgcggc aaggccttgc gcatccaggg tggccgggac
     3121 cgcgatcttc gaacttgtat ggagcagctg tgacagaagg gtcgacggtt cttcaaaagc
     3181 gaggtccgag gacg
//
LOCUS       DATMZD010000042         4300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_11154,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000042 DATMZD010000000
VERSION     DATMZD010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4300
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11154"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="VN478_00865"
     CDS             complement(<1..421)
                     /locus_tag="VN478_00865"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF014033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009235 - cobalamin metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP:cob(I)alamin adenosyltransferase"
                     /protein_id="HWQ21260.1"
                     /translation="MKPNIGAGDKGTTALLFGRRVSKAGPQPEAYGTVDELNSTLGLA
                     KSLCCQDYSREIIDGIQKDLGIVAAELATEPERHDELGKHGWIVSEEMVGKLEGQLAD
                     IEHKVTMPTKFIIPGATSGAAAIDMARAIVRRAERRVV"
     gene            complement(511..714)
                     /locus_tag="VN478_00870"
     CDS             complement(511..714)
                     /locus_tag="VN478_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21261.1"
                     /translation="MRIVTEAEIRSKIRTPEVGLVLCFPVGTRFSPSATDFIKQWRVE
                     IRFEESAGENVSRVPPRPPASFA"
     gene            complement(795..1550)
                     /gene="eutC"
                     /locus_tag="VN478_00875"
     CDS             complement(795..1550)
                     /gene="eutC"
                     /locus_tag="VN478_00875"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003971.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HWQ21262.1"
                     /translation="MGELKGFEHREMGVKNPYNREAIEAMMQATPARIATGRVGSRPP
                     VDAWLSFRLDEAAAKDAIWTRIPDEFLTKNGVALRVSTICTDKLDYVGHPEKGGILTK
                     EAEEAILAHCQKGAQVQVFMSEGLSSSAVMENLPDLLPALKHGFDAAGITFGTPFYVD
                     NGRVRLLNDVCRVLQPEVAIMLIGERPGLVTQTSLSAYMAYKAKMTDTDGERNCISNI
                     YNQGLNPMEAAATIVDIAKRMLDQKISGVALKL"
     gene            complement(1566..2954)
                     /locus_tag="VN478_00880"
     CDS             complement(1566..2954)
                     /locus_tag="VN478_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003386889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HWQ21263.1"
                     /translation="MNLTTTLQGQSYTFKSVKDVLAKSAEKRSGDELIGVAAQSERER
                     IAAKWVVANLTLKDIFENPVLPYETDEVTRVIINDTNKAVYNKIKGWTVADLRGYLLD
                     QNTTNEDIQRIRVGLTSEMISAVIRVMSVMDLVVASKKIINVTTANTTIGLPGTLSYR
                     NQPNHPTDDIDGIRASILDGLSYGAGDVVIGINPVDPTPDTLVRILEMTYGLIEKWQI
                     PTQNSALGHITGQMEAAKRGAHIGLFFQSIAGCEPANKGFGIDLKMMEEAWALAREYS
                     FCAGPNVMYFETGQGSEMSVGLDFGCDEQTLESRTYGYGRHFRPFCVNNVTGFIGPET
                     HLDTFQIAYGTLEDLFAGKMHGFPMGMAPCYVNHVDMNHNGQEMANLLAAAAGAVYYM
                     GVPMGDDVMLSYQDTTNHDDVSMREIFDRHPAPEFERWMERMGLLKNGKLTDRAGDPS
                     IFSAEPSDIFLW"
     gene            complement(3054..>4300)
                     /locus_tag="VN478_00885"
     CDS             complement(3054..>4300)
                     /locus_tag="VN478_00885"
                     /inference="COORDINATES: protein motif:HMM:NF024122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HWQ21264.1"
                     /translation="MKQVARIISVVLLVALLVSSFAGVAVAAGVPSGTYTGYTAYNWL
                     YSDWFIKKYGPTHPVAVYDWDNTCIYNDIMETMFRFQMFNLQYAMDKQAFNAIIPEAA
                     GKTLKDTDVLKLSADYGNVVLADLAADLEADYAFLYDNYIGTKKMTLEEVKATSEYQD
                     FLVKLPFMYDGLTDTPEIGAEYSYPWVCFLCEGMTKPEVQKMAVAALNDALARKSEKI
                     TMKAPTDLTRRASVASYAYKAGIRLAQGIIDMMSKQRATGWKVWVVTASFEPVIQAVA
                     AINGYDVPQDRVIGVRLQTDASGKYVAKLSEEPGYALTQRMGKATVIRNFIKQAPLFV
                     AGDSDGDFEMMTQFAETKLIYLVNRIKGGDIGTLYKEALPGMAKAGRVVLLQGRNENT
                     GLWRPFQETIKLGKTAPSPVVP"
BASE COUNT          824 a         1240 c         1241 g          995 t
ORIGIN      
        1 cgacgacacg tcgctccgcc cgccgcacga tcgcccgtgc catgtcgatg gcggctgctc
       61 cactcgtagc tcctggaatg atgaacttcg tcggcatcgt caccttgtgc tcgatgtccg
      121 cgagctgtcc ttccagcttc cccaccatct cctcagagac gatccagcca tgcttgccga
      181 gctcgtcgtg acgctctggt tccgtggcaa gttcagctgc cacaatgccc aggtccttct
      241 gaatgccgtc gatgatctcc ctcgagtagt cctgacagca tagagacttg gccaggccga
      301 gcgtcgagtt caattcgtct acagttccgt atgcttcagg ctgggggcca gccttcgaga
      361 cacgtcgccc aaacagcagg gctgttgtcc ccttgtcccc ggcaccaata ttcggtttca
      421 tgagcgtacc tccttgtttc tgcccgacag gcggggcggc atgactgcgt cgtggttccc
      481 gcctgtcggt gcattgtcca gaatccctgc ctatgcgaac gaggcaggtg ggcgaggagg
      541 gacacgtgat acattctctc cagccgattc ctcgaaacgg atctccacgc gccactgctt
      601 gatgaagtcc gtggcggacg gagaaaagcg cgtgccaacc gggaagcaca ggacgagacc
      661 cacttccggc gtacgtatct tgctccgtat ttctgcttcg gtgacgattc gcatacagcc
      721 tctcctcgcc gggctcgcgg cccacgggaa cctgcgggcg aagttcactc gcccgcagta
      781 gcatctatct ggctctagag cttgagtgct acgccgctga tcttctggtc cagcatgcgc
      841 ttggcaatgt cgacgatcgt tgctgctgct tccatgggat tgagaccctg attgtagatg
      901 ttcgagatgc agttcctttc gccgtcggta tccgtcatct tcgccttgta ggccatgtag
      961 gcgctgagac tggtctgtgt gaccagcccg ggacgctcgc caatcagcat gatggcgact
     1021 tcaggctgca gaacgcggca cacatcgttc aggagacgga cgcggccgtt gtccacatag
     1081 aacggggtac caaacgtgat gccagcagca tcaaaaccat gcttcagagc tggcagaaga
     1141 tctggaaggt tctccattac cgctgaagac gagagtccct cgctcatgaa gacctggacc
     1201 tgcgcacctt tctgacagtg cgcaaggatt gcctcttctg cttctttggt gaggatgccg
     1261 cccttctcag ggtgtccgac atagtcaagc ttgtccgtgc agatcgtgga gacgcgaagg
     1321 gcgaccccat tcttggtaag gaattcgtcc gggatcctgg tccagatagc gtccttggcg
     1381 gccgcttcgt ccagacggaa cgacaaccat gcgtctacag gaggtctgga tcccacgcgc
     1441 cctgtcgcga tgcgcgcagg agttgcttgc atcattgctt cgattgcttc acggttgtac
     1501 gggttcttga cgcccatttc gcggtgctca aaacctttta gttcacccat tgtggcctcc
     1561 tgtgcctacc agaggaagat gtcagacggc tcggccgaga agatggacgg atcgccagct
     1621 ctgtcagtga gcttgccgtt cttcagcaag cccatccgtt ccatccagcg ctcgaattcg
     1681 ggagccgggt gccggtcaaa gatctcgcgc atcgaaacgt cgtcatggtt ggtcgtgtcc
     1741 tggtagctca gcatgacgtc gtcacccatg ggaacgccca tatagtagac ggcacctgca
     1801 gctgcagcga gcaagttggc catttcctga ccgttgtgat tcatgtcgac gtggttgaca
     1861 tagcagggag ccatgcccat cgggaatcca tgcatcttgc ctgcaaacag atcttcgaga
     1921 gtgccatagg caatctggaa ggtgtcaaga tgggtctccg gtccgatgaa gccggtgacg
     1981 ttgttgacgc agaacggacg gaagtggcgg ccgtagccat aagttcgcga ctcgagcgtc
     2041 tgctcgtcgc agccgaagtc caggccgacc gacatctcgg agccctgtcc ggtctcgaag
     2101 tacatgacgt tgggtccggc gcagaacgag tactcgcggg caagtgccca tgcctcttcc
     2161 atcatcttga ggtcgatgcc gaagcccttg ttggccggct cgcaccccgc gatgctctgg
     2221 aagaacagac cgatgtgcgc accgcgcttg gctgcttcca tctggccggt gatgtgtccc
     2281 agtgctgagt tctgagtcgg aatctgccac ttctcgatga gcccgtaggt catctcaagg
     2341 atgcgcacga gcgtgtcggg agtcggatcg accgggttga tgccaatgac aacgtcgccg
     2401 gcgccataag acagaccgtc aagaatcgag gcacggatgc cgtcgatgtc gtccgtcggg
     2461 tggttgggct ggttgcggta tgacagtgtt ccaggcagac cgatggtcgt gttcgctgtc
     2521 gtcacgttga tgatcttctt ggaggcgacg acaaggtcca tgaccgacat cacacggatg
     2581 acggctgaga tcatttctga ggtcagtccg acgcggatcc gctgaatgtc ctcattggta
     2641 gtgttctggt caagcaggta gccacgcaga tcggcaaccg tccagccctt gatcttgttg
     2701 tagacagcct tgtttgtgtc gttgatgatg acgcgcgtga cttcgtcagt ctcgtagggc
     2761 agaacgggat tctcgaagat gtccttcagc gtcaggttgg cgaccaccca cttggcggcg
     2821 atacgctcgc gctctgactg agcggctacg ccgatcagtt catcgccgga acgcttctct
     2881 gcagacttgg cgaggacatc cttcacagac ttgaaggtgt acgactgccc ttgcagtgtt
     2941 gtagtgagat tcatctgttc ctcctgaaat tcctcatttg atgttgctta ccagtgtcct
     3001 ctgttttcga ggtcgccaaa cagtcgctgc ttctgccctt ctctgtacga tcgctacggc
     3061 acgacaggac ttggcgcggt ctttcccagt ttgatggttt cctggaatgg acgccacagt
     3121 cccgtgttct cgtttcgacc ctgaagaagc actacgcggc cggcctttgc catgccaggc
     3181 agagcttcct tgtacagcgt accgatgtct ccgcccttga tgcggttcac gagatagatg
     3241 agcttggttt cggcaaactg ggtcatcatc tcgaagtcgc cgtcactatc gcccgcgacg
     3301 aacaaaggag cctgcttgat gaagttccgg atgacagttg ccttgcccat ccgctgagtc
     3361 aatgcataac ctggctcttc gctgagcttg gcaacgtact tgccggaagc gtccgtctgc
     3421 aagcgtacgc cgatgacacg gtcctggggc acgtcgtagc cgttgatggc tgcaacggcc
     3481 tgaatgaccg gctcaaatga tgcagtgacg acccacacct tccatcctgt ggcccgctgc
     3541 ttcgacatca tgtcgatgat gccctgcgca agacggatgc cggctttgta tgcataggag
     3601 gcgacactgg ctctgcgagt cagatcagtc ggagccttca tcgtgatctt ctcgctcttg
     3661 cgagcgagag catcgttcag cgcagcaaca gccatcttct ggacttccgg cttggtcatg
     3721 ccttcacaga ggaagcagac ccagggatac gagtattcgg ccccgatctc gggagtgtcg
     3781 gtcaacccgt cgtacatgaa cggcagcttg acgaggaagt cctggtattc ggatgtggcc
     3841 ttgacctctt ccagagtcat cttctttgtg ccgatgtagt tgtcatacag gaacgcgtaa
     3901 tcggcctcga ggtcagcagc cagatcggcg aggacgacgt tgccgtagtc agcagacagc
     3961 ttgaggacgt cggtgtcttt aagcgtcttt cccgccgcct ccgggatgat ggcgttgaag
     4021 gcttgcttgt ccatggcgta ctgcaggttg aacatctgga agcggaacat cgtctccatg
     4081 atgtcattat agatgcaggt gttgtcccag tcatagacag caactggatg tgttgggccg
     4141 tacttcttga tgaaccagtc cgagtagagc cagttgtagg cagtgtagcc tgtgtaggtt
     4201 cccgacggca cgcctgcggc cacggctacg ccggcaaacg acgagacaag cagtgcaacg
     4261 agcagtacga ccgagatgat cctggcaact tgcttcatac
//
LOCUS       DATMZD010000043         2769 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_122114,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000043 DATMZD010000000
VERSION     DATMZD010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122114"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            245..937
                     /locus_tag="VN478_00890"
     CDS             245..937
                     /locus_tag="VN478_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWQ21265.1"
                     /translation="MRKILVVEDEENIRTLEKLYLQKEGYEPVLAATGREALRLFDEQ
                     KPDLVILDLMLPELNGEEVCREIRKKSTVPIIMVTAKSDEIQKLEGLEIGADDYVTKP
                     FSPRELMARIKAALRRYDWERLADTAPVEKLVFKDIVVYPGKHRVVKAGKDIMLTPKE
                     FEVFTLLVKANGEPVSRSSLIKEIYGGSDDEVFDRTIDAYIKNIRKKIQDDPGNPTYI
                     ESIYGVGYRILV"
     gene            947..2113
                     /locus_tag="VN478_00895"
     CDS             947..2113
                     /locus_tag="VN478_00895"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWQ21266.1"
                     /translation="MKKTSSFRARLILSYAALLILVMVLSISIADAFFIRAFKTFLSG
                     GYGSPFGIKSMIIEPESYLLTTTYLKFHDMMRLSVWIGGCAVMLIALGVAGWFTRRFS
                     QPISGFASFADRIGHGDYDAQISNNQGLREFAELSDSLNHMAHELKCRDEVEQELFSN
                     LSHELRTPVTVIKGYMEALDAGMISNPQELHGAAEAISQETANLEIMINELRQLAQID
                     NNAVMPEESDFDVDEVLGYIWRRFAPIAAGRGVLLSHQFDAHVKVHLDRQLLDRAVAN
                     LLSNAITATPSGNSVTLSSRVNDSRGVSIVVEDTGSGIPQEDLAHVFDRFFRGDRSRN
                     RRSGGLGLGLPIARDLVEIEAGHLDIKSTVGIGTRVTVSYPRSAVARNEVLQAS"
     gene            2195..2656
                     /locus_tag="VN478_00900"
     CDS             2195..2656
                     /locus_tag="VN478_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014452910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HWQ21267.1"
                     /translation="MAADNMLHAEVRQTEGLTFVARGPSNHWVVLDTSESGGGHGAGS
                     SPMELLLMALGGCTGMDVVSILAKMKESHAGFRVELSGERAEEHPHRFTRIYVDYYLT
                     GDSILAENISKAIKLSAEKYCSVGASLMPGVALVHRYHIIRSTGEETGEVV"
BASE COUNT          587 a          770 c          816 g          596 t
ORIGIN      
        1 gcgtaccgct cgctcgtttc gccccgtcga cttgaatggc ggggcgaaac catgttgtcg
       61 gtgttgcata ccagcggcag gatgatagta tggtttcatg ttcttcacaa ttgggaacgg
      121 gaatccagtt gaatgccact ctctggcgtc actgagctac aggcgctatt catgctgact
      181 tgcggttcac tgtctgttca cacacggcga tgacacaatc atccatcgag ggtagaatac
      241 aaccatgaga aagatactgg tagtcgaaga cgaagagaac atccgcacac tcgagaagct
      301 gtacctgcag aaggaaggct atgagcccgt tctcgccgct accgggcgtg aggcgctgcg
      361 cctgttcgac gagcagaaac ccgatctcgt catcctggac ctcatgctgc cggaactcaa
      421 cggcgaggag gtctgccgcg agatccgcaa gaagtcgact gtaccgatca tcatggtgac
      481 cgccaagagt gacgagatcc agaagctgga aggcctggag atcggcgcgg acgactacgt
      541 cacgaagccc tttagtccgc gagagctcat ggctcgcatc aaggcagccc tcagacggta
      601 tgattgggag cggctggccg acacggcgcc cgtcgagaag ctggttttca aggacattgt
      661 tgtctacccg ggcaaacacc gtgtggtcaa ggctggcaag gacatcatgc tcacgccgaa
      721 ggaattcgaa gtgttcacgc tgctcgtcaa ggccaacggt gaaccggtct cgcgctccag
      781 ccttatcaag gagatctacg gcggaagcga tgacgaagtc ttcgaccgta ctatcgacgc
      841 ctacatcaag aacatccgca agaagatcca ggatgacccc gggaacccga cctacatcga
      901 atccatctac ggagtcggat atcgcatcct ggtgtaacgt gacttgttga agaagacgtc
      961 cagctttcgc gccaggctga tcctcagcta cgctgcgctg ttgatattgg tcatggtgct
     1021 ctccatcagc atagcggacg ccttcttcat acgtgctttc aagaccttcc tctcaggcgg
     1081 atatggatct ccgtttggca taaagtcgat gatcatcgag ccggagagct acctcctgac
     1141 gaccacgtac ctcaaattcc atgacatgat gcgtctctcc gtatggatcg gtggatgcgc
     1201 cgtcatgctg atcgcgcttg gcgtcgcggg atggttcacg cgacggttct cccagcccat
     1261 ctcaggattt gcgagctttg cggatcgcat cggtcatggc gactacgatg cacagatctc
     1321 caacaatcag ggattgcgcg aatttgcgga gttgtccgac tcactgaatc acatggccca
     1381 cgagttgaag tgtcgcgacg aggttgagca ggagctcttc tccaaccttt cacacgaact
     1441 tcgcacgccc gtgaccgtca tcaagggata catggaggct cttgatgcgg ggatgatctc
     1501 gaacccgcag gaattgcatg gcgcagcaga ggcgatctcc caggagactg ccaatctcga
     1561 gatcatgatc aacgagcttc ggcagcttgc gcagatcgac aacaacgctg tcatgcctga
     1621 ggagtcggac ttcgatgtcg atgaggtgct ggggtatatc tggcgcagat tcgctcctat
     1681 cgctgcgggt cgtggcgttc tgctgtcaca ccagtttgac gctcacgtga aggtccatct
     1741 tgaccgtcag cttctggaca gggctgttgc caatctattg agcaacgcca ttactgcgac
     1801 accatcgggc aactccgtga ctctctccag ccgagtcaac gattcgcgag gcgtcagcat
     1861 agtcgtcgag gatacggggt ctggtattcc ccaggaggat ctggcacacg tattcgaccg
     1921 attcttcagg ggcgatcgtt cgcgcaacag gaggagcgga ggtctgggtc tgggccttcc
     1981 gatcgccagg gatctcgtgg agatcgaagc cggtcatctg gacatcaaga gtacggtggg
     2041 tattggcaca cgcgtaaccg tctcgtatcc acgatctgcc gtggcacgga acgaagttct
     2101 tcaggcctcc tgaggaaccg gagtctcggg catcagccgc agtgccagac cgaaatctgc
     2161 gaccctcagg ggccgcgttc tgaagggggt gtccatggcc gcagacaaca tgttacatgc
     2221 tgaggtccgt cagaccgaag gactcacgtt cgtggcgcgc gggccgtcca atcactgggt
     2281 cgttctcgac acgtctgagt caggcggcgg tcacggggct gggtcgagcc ctatggaact
     2341 gctgctgatg gctcttggag ggtgcacggg tatggatgtc gtatccatcc tcgccaagat
     2401 gaaagagtca catgcgggat ttcgtgttga gttgtccggc gaacgtgcgg aggagcaccc
     2461 tcatcggttc acccgcatct atgtcgacta ctacctgacg ggtgacagca ttctggcgga
     2521 gaacatctcc aaggccatca agttgtccgc tgagaagtac tgctcggttg gcgcatcatt
     2581 gatgcccggt gttgcgctgg ttcacaggta tcatatcatt cgcagcaccg gcgaggaaac
     2641 cggcgaggtc gtctgacctg accttgccga gcatacgggt actccttggt cgccggcact
     2701 ttgcagtgtg ccggcgacct gcgtcctgag gccgtcgcta cttgtcgtga ttgactggca
     2761 ggacgggtc
//
LOCUS       DATMZD010000044         4022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_102051,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000044 DATMZD010000000
VERSION     DATMZD010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102051"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="VN478_00905"
     CDS             complement(<1..525)
                     /locus_tag="VN478_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21268.1"
                     /translation="MNHPEATQAALSILRKGSGFQWYVITMLIFVIYIYATEIERKNW
                     KGVAAGMALYMIHWFVEIINALIQHFSGHALWTVPGGTSFLLLIGVGVELSLMFSVAG
                     LASSKMLLTDPKAKILGINNRWFIGIANAALASILEIFLVKTPVFVWVYRWWGAIPVF
                     VTVYIPFFVVANLCY"
     gene            complement(597..1736)
                     /locus_tag="VN478_00910"
     CDS             complement(597..1736)
                     /locus_tag="VN478_00910"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HWQ21269.1"
                     /translation="MAIMVAVATALPMVGVQAPTYPPNGTVIPARPTVLGRDVITLRE
                     TYHLPALELCVINTDGIVDVAADGVRKAGGTDAVTLQDLFHLGSMTKAMTATMLATLV
                     QEGKLSWDMTMVQAFPSLASVMDRRYRDVTLEQLLTHTSGLPRYTTDPEWASIPPFTG
                     TPAQQRQAFARMLLTEPPIGPTGIYRYSNAGFSVAAAIAERVTGESWEQLMQERVFGP
                     LNIRAAYGWPLMSGVGEPWGHRIKNGAVTPHDPADHYKVPTVLTPAGDVSMSILDYSV
                     FARLHLAGLGNIDGAVLSAAEIQRLHQPVLAYSCGWHEELIDGVPTSWHRGTCDTFDT
                     FVLLQPSRGIGVIAFTNADGDNTAVLALFAELSRIAAMYAPQQAG"
     gene            1955..2362
                     /locus_tag="VN478_00915"
     CDS             1955..2362
                     /locus_tag="VN478_00915"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HWQ21270.1"
                     /translation="MEFGLEFERTSEILGTARSRGVELVFDTGHGIDKQGMWPMEVLV
                     ASLGGCLNLDVGAALQMSGYTLDRLTMTLSGHRDDDIPRIDRISCRIDVWSSNLSQAA
                     ADEILAYALGRSTICNTLADTVTLEITISPHES"
     gene            2430..3026
                     /locus_tag="VN478_00920"
     CDS             2430..3026
                     /locus_tag="VN478_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21271.1"
                     /translation="MTRRTIGLVSIVLVLVIGLGALIAVPAVRAAVTAPVGAADKAAV
                     DARRATLKTLGQQIRETAKAVQEKVNAAKAAGKDLTAFRAAIVTALKGGRHLRLDVAR
                     MRKPQLTEAQRTQLKTAQMAIEAQRKNLQARTKAGAPQAELDALKAGIKAAVQAREAL
                     MKSFRVSGQALVIQRLDNLVVEATKELTALQSLLSRLP"
     gene            3153..>4022
                     /locus_tag="VN478_00925"
     CDS             3153..>4022
                     /locus_tag="VN478_00925"
                     /inference="COORDINATES: protein motif:HMM:NF040322.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HWQ21272.1"
                     /translation="MIHTRVMVLSFIVLVLCCGLCGCIPATPSTGSGTTSLRVLSYNV
                     ENAFDDRVDGTEYSDYSTSSNYYADRLWQAKIDHIHEVLQAADNPQIVGLVEVENQRM
                     AAMILDSVRDLGYRDMAVTTGGSVTQCVLISSFPIQSVASLPTTTGVRDILTASVDIE
                     GTPVHVLVNHWKAKDSGETESVRIKNARVARAEVDRLLKQDPAADIVLIGDFNSTETE
                     HAATGKTTGINDVLRTTGDRTSMTVQGTRSLYDPWFEMPTSQRGSEIHGSKWESLDHI
                     IMSPGMFDRTGLSY"
BASE COUNT          817 a         1257 c         1185 g          763 t
ORIGIN      
        1 atagcaaagg ttcgcgacta cgaagaaggg gatatacacg gtgacgaaca ccgggatcgc
       61 accccaccac cggtagaccc agacgaacac gggcgtcttc acgaggaaga tctccaggat
      121 cgacgcgagt gcggcgttcg caatgccgat gaaccagcgg ttgttgatac ccaggatctt
      181 cgccttcggg tccgtcagca gcatcttgct tgatgctagt cccgccacgg agaacatgag
      241 cgacagctcg acgccgacac cgatcagcag aagaaatgat gtccctccgg gaaccgtcca
      301 cagagcatgc cccgagaaat gctggatgag ggcgttgatg atctccacga accagtggat
      361 catatacagg gccatcccgg cagcgacacc cttccagttc ttccgctcga tctcggtggc
      421 gtagatgtag atgacgaaga tcagcatcgt gatgacgtac cactggaacc cgcttccctt
      481 gcgaagaatg ctgagtgccg cctgtgtcgc ctccggatga ttcatcccac gctccttctc
      541 tcttcagctg tcctgacgat ctgcacaatg ccggcagtat accaggaaaa ggcggtctac
      601 ccagcctgct gaggcgcgta catcgcggct atcctggata gctctgcaaa cagtgcaagc
      661 actgcggtat tgtcgccgtc ggcgttggtg aatgcgatca cgccgattcc cctcgaaggc
      721 tgcagcagta cgaacgtatc gaacgtgtcg caagtccccc ggtgccagct tgtcggtacg
      781 ccgtcgatca gctcctcgtg ccatccacag ctgtacgcca ggaccggctg gtggagacgc
      841 tggatctccg ctgccgacag cacggcgccg tcgatgttcc ccagcccggc gaggtgcagc
      901 cgagcaaaca cactgtagtc caggatcgac atgctgacat cgccagccgg ggtgaggacg
      961 gtgggtacct tgtagtggtc cgcggggtcg tgcggcgtga ctgcgccatt cttgatccgg
     1021 tggccccagg gttcacccac accggacatg agcggccagc cgtacgcggc tcgtatgttc
     1081 agtggaccga atacgcgttc ctgcattagt tgttcccacg actcgccggt gacccgctcg
     1141 gcgatcgcag cagccacgga aaatccagcg ttagagtacc gatagatgcc cgtcgggcca
     1201 atgggtggtt ccgtcagaag catgcgcgca aatgcctgtc gctgctgcgc cggcgtccca
     1261 gtgaagggtg ggatgcttgc ccactcgggg tccgtcgtgt accgcggcag cccggacgtg
     1321 tgtgtcagca gctgctccag cgtcacgtca cggtaccgtc tgtccatgac ggacgccagc
     1381 gacggaaacg cctggaccat cgtcatgtcc caactgagct tcccctcctg gaccagcgtc
     1441 gccagcatcg tggccgtcat cgctttggtc atcgacccaa gatgaaacag gtcctgcagt
     1501 gtgactgcgt ccgtgccacc ggccttgcgc acaccatcgg cggcaacgtc gacgatcccg
     1561 tccgtgttga tgacacagag ttccagagca ggaaggtgat atgtctcgcg cagggtgatg
     1621 acgtcgcgtc caagaaccgt cggccgtgcc ggaatcacgg tcccgttcgg cggatatgtc
     1681 ggcgcctgaa cgcccaccat gggcagagca gtagcgacgg caaccattat cgccatgacc
     1741 agtccccgcc ccaccaatgc agtcaatcgt cgcatatgca tctatcaatt gtagcactca
     1801 ccatggccgc cgtcccttca ccgtcacgct gacgacagcc tgaccccgga gtccctcctt
     1861 ctccgattct tgccgattgc gtctcgagcg gacgttgtga ttcgcccctc ctggcgctat
     1921 gatagactaa cagtagtctg cgtcggaggc aactatggag ttcggacttg agttcgagcg
     1981 caccagtgag atccttggca ccgcccggtc gcgtggtgtc gagctggtct ttgataccgg
     2041 acacgggatc gacaagcagg gcatgtggcc catggaggtc ctcgtagcgt cgctgggcgg
     2101 ttgcctgaac ctggacgtcg gcgcggcgct gcagatgtcc ggctacacgc tggacagact
     2161 gaccatgacg ctttcaggcc accgggatga cgatattccg cgcatcgacc gcatctcctg
     2221 tcgtatcgac gtctggtcca gcaacctctc gcaggcggcg gcagacgaga tcctcgccta
     2281 cgcgcttgga cgcagcacca tctgcaacac actggcagac actgtgaccc tcgagatcac
     2341 gatatccccg cacgagtcgt gaacacttca ccgacctcca cagtccaata ggcgaaatca
     2401 gaaacaattc tctagttgtg gaggtacata tgacacgaag gactattgga ctggtgagca
     2461 tcgttcttgt gctcgtcatt ggtcttgggg cgttgatagc cgttcctgcc gtccgcgctg
     2521 ctgtgactgc gcctgtaggc gccgccgaca aggctgctgt cgacgcccgc cgagccacac
     2581 tcaagacact cggtcaacag atccgcgaaa ccgcgaaggc cgttcaggag aaagtcaatg
     2641 ccgcaaaggc cgcgggtaag gatctgacgg cgttcagggc agctattgtc actgcactca
     2701 agggcggccg gcatctgcgc cttgacgttg cccggatgcg gaagccacag ctcactgagg
     2761 cccagcgcac gcaactcaag accgcacaga tggctattga ggctcagcgc aagaacctcc
     2821 aggcacgaac caaggccggt gctccacagg ccgagctcga cgcgctgaag gctggcatca
     2881 aggctgctgt gcaggcacgc gaagcgctga tgaagtcgtt cagggtttcg gggcaggctc
     2941 tggtgatcca gcgtctggac aacctcgttg ttgaggcgac caaagaactc acggccctgc
     3001 agagtctcct ctctcggctt ccatagcgac tgaccaatcg agcatggcgg gcgggtgctg
     3061 tcgcacctgc ccgcgttttc atttcgggga gctcatgtat actgtgctat tctgtcgggt
     3121 ggtactgcaa gcccgcgaac gaaggagctc cgatgatcca tacgcgcgtc atggtcctgt
     3181 cattcatcgt gctggtcctc tgctgcggtc tatgtgggtg tatccctgca actccctcca
     3241 ctggctccgg cacaacgagt ctgcgtgtcc tcagctacaa cgtggagaac gccttcgacg
     3301 accgcgtgga tggcaccgag tactcggact acagcacatc gtcgaactac tacgcggacc
     3361 gcctgtggca ggcgaagatc gaccatatcc atgaggtcct gcaggcagcg gacaaccccc
     3421 agatcgtggg cctggtagag gtggagaacc agcgtatggc cgcgatgatc ctcgactccg
     3481 tccgcgacct gggctaccgg gacatggccg tcacgacggg agggtctgtc acccagtgtg
     3541 tgctcatctc ctccttcccg atccagtccg tggcgtctct gccgacgacc accggagtgc
     3601 gagacatcct cactgcatct gtggacattg agggcactcc tgtccatgtc ctggtcaacc
     3661 actggaaagc caaagactcg ggcgagacag aaagcgtgcg catcaagaac gcaagggtcg
     3721 cgcgtgcgga ggtcgaccgc ctgttgaaac aggatcctgc agcagatatc gtcctgatcg
     3781 gagatttcaa cagtaccgag accgaacacg cggcaacagg caagacgacg ggcatcaacg
     3841 acgtcctgcg gacgacgggc gaccgcacct ccatgacagt ccagggcact cgatccctgt
     3901 acgatccctg gttcgagatg ccgacgtcgc agcggggctc ggagatccat ggctccaagt
     3961 gggagagtct ggaccacatc atcatgagcc cgggcatgtt cgaccgaacc ggcctgagtt
     4021 ac
//
LOCUS       DATMZD010000045         3313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_71897,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000045 DATMZD010000000
VERSION     DATMZD010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71897"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1469
                     /locus_tag="VN478_00930"
     CDS             <1..1469
                     /locus_tag="VN478_00930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWQ21273.1"
                     /translation="NVGAERMLGYAAADVMNKITPADISDPEEVIARAKALSAELGTP
                     ITPGFEALVFKASREIEDIYELTYIRKDGSRFPAVVSVTALRDDQGAIIGYLLIGTDN
                     TARKQIEEDRKLLDQRLRDQQFYTRSLFESNIDALVTTDPSGIISDVNRQMEELTGCT
                     RDELIGSRFTRYFTDPERAEASLKLVLNQKKVTNYELTARARDGRETVVSYNATTYYD
                     RDRRLQGMFAAARDITERKVLEESVRRRSAELSAANKELEAFSYSVSHDLRSPLRSID
                     GFSKAFLEDYGTLVPEEGRADLERVWRATERMGHLIDDMLLLARVTRSEMHVQTTDIS
                     AMAAEVAGDLRQENPQRDVQLTIEPGMTVTGDPQLLHIVLVNLLGNAWKFTSQREQAH
                     ISMGTVQDPEHGLAFFVRDDGAGFDQKYKDKLFVAFQRLHGQDEFPGTGIGLATIQRV
                     IRRHGGDVWAEGEVNRGATFYFTIPDLYVSASAKEERP"
     gene            1466..1909
                     /locus_tag="VN478_00935"
     CDS             1466..1909
                     /locus_tag="VN478_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWQ21274.1"
                     /translation="MSGKIILLVEDNPDDVALTLRALKKNNIANDVVVAGDGEEALEY
                     LTATGGYAGRTVADVPQVVLLDLKLPKVGGLEVLRAMRADPRTSMLPVVILTSSTEER
                     DIVASYQLGANSYIRKPVDFDQFVDAIRQLGLYWLVLNQAPSAGQ"
     gene            1924..>3313
                     /locus_tag="VN478_00940"
     CDS             1924..>3313
                     /locus_tag="VN478_00940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWQ21275.1"
                     /translation="MKPTLRVLIIEDSVDDALLAVRAVEHAGYAVHAGRVQTVDELRG
                     ALRQGPWDLVLADYSLPHFSAPEALAILNETGLDLPFIVVSGKIGEDVAVQLMKDGAT
                     DYVMKDHLSRLGPAVQREMHDAEERRHKREAEQALKEAEERYRALVESQTDLIVKTGP
                     DQRFGYANPTYCALFGKSLQELTNSSYQPLIHPDDVASVTDATAGLFHPPYSCRYEAR
                     VMTVGGWRWIEWNSRAELDSKGTVVALLGSGRDITDRKQMEERLRMSEFDLRQAQAIA
                     HVGSWRWDVAQDKLTWSEEMHRIYGIDPQAFTGSWHDVIARSVHPDDRARVEESNRTV
                     VEGHNPQPLEYRIVRPDGSVRTVWDETGEMTLDDKGQVCSLTGVVLDITDRRQAEAER
                     AAMQEQLLQAQKMEAIGELAGGIAHDFNNLLTGILGNISIIKGDLPASDPLHSYLLAA
                     ETAAHQAADLTKG"
BASE COUNT          694 a         1051 c         1031 g          537 t
ORIGIN      
        1 tcaacgtagg ggccgagcgc atgcttggat acgctgctgc cgacgtcatg aacaagatca
       61 caccggccga catctccgac ccggaagagg tgatcgcccg tgccaaggcg ctcagcgcgg
      121 agctcggcac acccatcacg ccggggttcg aggcactggt gttcaaggcc tcgcgcgaga
      181 tcgaggacat ctacgagctg acgtacatcc gcaaggatgg cagccggttc cccgcagtcg
      241 tatcggtcac ggccctgcgg gatgatcagg gcgccattat cgggtatctg ctcatcggca
      301 cggacaacac cgcacgaaag cagatcgagg aagaccggaa attgctcgat cagcgcctgc
      361 gcgaccagca gttctacacc cgttccctgt tcgaatccaa catcgacgca ctggtgacca
      421 cggacccatc cggcatcatc tcagacgtca acagacagat ggaggagctc accggctgca
      481 cgcgcgatga gctgatcgga tctcggttca cacgctactt caccgatccg gagcgcgccg
      541 aggcgagcct gaaactggtg ctgaaccaga agaaagtcac taactatgag ctcaccgcac
      601 gcgccaggga cggtagggag actgtcgtgt cctacaacgc gaccacctac tacgaccggg
      661 acaggagact gcagggcatg ttcgccgctg cgcgcgacat caccgagcgg aaggtgctgg
      721 aggaaagcgt acgtcggcgg agtgcagagc tctctgccgc caacaaggag ctggaggcgt
      781 tcagctactc ggtctcacac gacctgcgat ccccgctgcg cagtatcgac ggattcagca
      841 aggccttcct tgaggactat ggcaccctgg ttcccgagga ggggcgcgcg gacctggaac
      901 gcgtctggcg cgctactgaa cgcatggggc acctcatcga cgacatgctg ctgctggcgc
      961 gtgtcacccg gagcgaaatg cacgtgcaga caactgacat aagcgccatg gctgccgaag
     1021 tggccggcga cctgcgccag gagaaccctc agcgcgacgt ccagctcacc attgagcctg
     1081 gcatgacggt caccggagat ccacagttgc tgcacatcgt gctggtcaac ctgctcggca
     1141 atgcctggaa attcacatcg cagcgagagc aggcgcatat cagtatgggc accgttcaag
     1201 acccggagca cggcctcgcc ttcttcgtac gggacgatgg agccggattc gaccagaagt
     1261 acaaggacaa gctgtttgtg gccttccagc gactgcacgg gcaagatgaa ttccccggaa
     1321 cgggtatcgg gctggcgacg atccaacgcg taatccggcg ccacggcggc gacgtatggg
     1381 ccgagggcga agtcaaccgg ggagcgacgt tctacttcac catccctgac ttgtatgtat
     1441 cagcttccgc caaggaggaa cgaccatgag tggaaagatc atcctgctcg tcgaggacaa
     1501 ccctgacgac gtagccttga cgctgagggc gctcaagaag aacaacatcg ccaacgatgt
     1561 cgtggtcgca ggcgacgggg aggaagccct cgagtatctg acagccaccg gagggtacgc
     1621 cggacgtact gttgccgacg ttccgcaggt cgtcctgctc gacctgaagc tgccaaaggt
     1681 aggcgggctc gaggtcctgc gggcgatgag ggcggaccct cggacatcta tgctgcctgt
     1741 ggtaatcctg acctcgtcca cggaagagcg agacatcgtc gccagctacc agctgggagc
     1801 caacagctac atccgcaagc ccgtggattt cgaccagttt gtcgacgcca tacgccagct
     1861 cggcctgtac tggctggtgc tcaaccaggc cccctcggcc ggccaatgac atcgccggat
     1921 tccgtgaaac cgaccctccg cgtcctcatc atcgaggaca gcgtcgacga cgccctgctg
     1981 gcggtccgcg cggtcgagca tgcaggctac gcagtccatg cggggcgcgt ccagacggtc
     2041 gacgaactgc ggggcgcact gaggcaggga ccctgggacc tggtgctggc cgactacagc
     2101 ctgccacact tctcagctcc tgaggcactg gccatcctga atgagaccgg gttggatctt
     2161 ccgttcattg tcgtgtcggg caagatcggc gaggatgtcg cggtccagct gatgaaggac
     2221 ggcgcgaccg actacgtgat gaaggaccac ctgtcacggc tgggccccgc tgtccagcgt
     2281 gagatgcacg atgccgagga gcgccgtcac aagcgcgagg cggagcaggc gctgaaggag
     2341 gctgaagaac gctaccgcgc cctcgtcgag agccagactg acctcatcgt caagaccggt
     2401 cctgaccagc ggtttggcta cgcgaacccc acctactgcg ccctgttcgg caagagcctg
     2461 caggaactga cgaattcaag ctaccagcca ctgatccatc ctgatgatgt cgcgtccgtc
     2521 actgacgcca cggccggcct gttccatcca ccctactcgt gccgctatga ggcacgggtc
     2581 atgaccgtgg gcggctggcg atggatcgaa tggaactccc gcgctgagct ggacagcaag
     2641 ggcaccgtcg tcgccctcct gggctccggc cgcgacatca ctgaccgcaa gcagatggag
     2701 gaaaggttgc gcatgtcgga gttcgacctc cgccaggcgc aagccatcgc gcacgttggc
     2761 agctggcgct gggacgtggc gcaggacaag ctgacctggt ccgaagagat gcaccgcatc
     2821 tacgggatcg atccacaggc tttcactgga tcctggcatg atgtcatcgc gcgttccgtc
     2881 caccctgacg acagggctcg cgttgaggaa tccaacagga cggtcgtcga gggccacaac
     2941 ccgcagcctc tcgaataccg catcgtgcgg cccgacggct ccgtacgcac cgtctgggac
     3001 gagaccggag agatgaccct ggacgacaag ggccaggtct gctccttgac cggcgtcgta
     3061 ctcgacatta cggaccggag gcaggccgag gcggagagag cggcgatgca ggagcagctg
     3121 ctgcaagcgc agaagatgga agccatcggc gaactggcag gcggcatcgc tcatgacttc
     3181 aataacctgc tgaccggtat cctgggcaac atctccatca tcaagggcga tctgccggca
     3241 tccgacccgc tgcactccta cctgctcgcg gccgagactg ccgctcatca ggccgccgac
     3301 ctcacgaagg gac
//
LOCUS       DATMZD010000046         3405 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_102129,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000046 DATMZD010000000
VERSION     DATMZD010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102129"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="VN478_00945"
     CDS             complement(<1..580)
                     /locus_tag="VN478_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21276.1"
                     /translation="MTRKLAGTRSRLVFIAVLLFMAAIACGSLMRSATHGAADPDTQE
                     ITTAEVSTTIRERIADLDAKAITNETVRSAVADQLPALGARGFIRVHIIVSKNVDVLV
                     GTDYNLDPVLLSTTGEGFWHYRTSLAPVNAPDDLQPLYVTYLSTGMGSYWGELIIGSA
                     GPTAAQTRASLSLMIAMWVCMCLAAVCVIMVFI"
     gene            complement(570..1268)
                     /locus_tag="VN478_00950"
     CDS             complement(570..1268)
                     /locus_tag="VN478_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWQ21277.1"
                     /translation="MRVLVVEDEPTMLSALSDALTHERLAVDIAADGSDALGRVHVEQ
                     AYDLILLDLMMPRMDGLAFLRTLRSEGYVMPVLILTARDLTRDKVNGLDLGADDYLTK
                     PFDLAELLARVRALLRRNGDRKTSVLSAGAVELDTVTHVCRMRGVTVSLATKEEQLLE
                     YLLRNQGRIVPREELEDHLWDAKASLWSDTLRTHIRYLRAKLDTDPAHPLITTVRGLG
                     YGIVAPSKEDEHDS"
     gene            1465..2181
                     /locus_tag="VN478_00955"
     CDS             1465..2181
                     /locus_tag="VN478_00955"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HWQ21278.1"
                     /translation="MSVRKPEGEQRTSPVAGAYVLVRSRRRSIGLEVRPDATLVVRAP
                     TRTPVWFVESLLREKAGWIEDKLAKARSHVSLLPRHDFLTGERFPYLGREWPFAVVAF
                     QKTPLTFDEKSGFSLDMAAFDRGEVVFEEWYRSRARELLADRVLHYAPLVGVAIPHLR
                     ITGAERRWGSCSTTGTVSFAWRLIMAPMDVVDYVVVHELAHRREMNHSVRFWTLVASV
                     MQDYDTRRRWLKEHGGMLTI"
     gene            2330..2602
                     /locus_tag="VN478_00960"
     CDS             2330..2602
                     /locus_tag="VN478_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21279.1"
                     /translation="MKRITSLLVAFLLVGMLVVAAGCGKAAAAPATTDPASTTDQTRA
                     TDQTQSTDQNGTTYEDQTTDGTQAPGTETTPSGDQTQVTPQGGTVQ"
     gene            complement(2663..>3405)
                     /locus_tag="VN478_00965"
     CDS             complement(2663..>3405)
                     /locus_tag="VN478_00965"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWQ21280.1"
                     /translation="RRDPLRVLPTPLWKTVARLQSLECRFIADNEQTTGVELREAGKL
                     LMYGTRTQDPLKAASVKLDGVPTVWIHNDFVAPLAPWFDRKEAFFRLIHAGSELPLHA
                     TVPGYAIREVDTTSEVPLVATILHACYDTATPGTDEIAAWTKRAVFDPHAWLWIIDRK
                     ERRPVALGIAETDRITEEASLEWIQVLPAFRSLGLGTMLVQELVYRLEQQVNFTTVAG
                     QLKNPSEPDHLFRRCGFAGRDVWWVLRR"
BASE COUNT          647 a         1002 c         1080 g          676 t
ORIGIN      
        1 ggatgaacac cataatgaca cagacggccg caagacacat acaaacccac atggcgatca
       61 tcaaggacaa cgatgccctg gtctgagcgg cagtgggacc tgcgctgccg atgatgagtt
      121 caccccagta agagcccatg ccggtactca ggtacgtaac gtacagtggc tggaggtcgt
      181 ccggcgcatt cacgggtgct aacgacgtgc ggtagtgcca gaagccctcg cctgtcgtgc
      241 ttagcaggac tgggtcgagg ttgtagtcgg tgccgacgag aacgtcgacg ttcttgctta
      301 cgatgatgtg cacgcggatg aaccctcgtg caccgagagc tggcagctgg tctgcgaccg
      361 cactccgaac cgtctcgttc gttatcgctt tcgcatccag gtccgcgatg cgctcgcgga
      421 tggtcgtgct gacttcggct gtcgtgatct cctgggtatc cgggtcggca gcaccgtgtg
      481 tagcgctgcg catgagactg ccacaggcga ttgcggccat gaacagcaga actgcgatga
      541 agaccagacg agagcgcgtt cctgcaagct tacgagtcat gttcgtcctc cttggagggg
      601 gcgacgatgc cgtagcccaa tccacgtaca gtcgtgatca atgggtgtgc cgggtcagtg
      661 tccagcttgg cgcgcaggta tcggatatgc gtgcgcagcg tgtctgacca gagagacgcc
      721 ttggcgtccc acaggtggtc ttccagctct tcgcgtggga cgatgcgtcc ctggttgcgc
      781 agcagatact ctagcaactg ctcttccttt gtggccagcg acaccgtcac tccgcgcata
      841 cggcagacat gggtcacggt atcgagttca actgctcctg ccgacaggac cgacgtcttg
      901 cggtctccgt tcctgcgcag cagtgcgcgc acgcgtgcca gcagctcagc caggtcgaag
      961 ggtttggtca ggtagtcatc cgcgcccagg tccaacccat tcactttgtc tctggtcaga
     1021 tcacgtgccg tcaggatcag aaccggcatg acatatccct cggaacgcaa ggtacgcaga
     1081 aatgcgagac cgtccatgcg gggcatcatc aggtccagca ggatcaggtc gtatgcctgc
     1141 tcgacatgga ctcggccgag tgcgtcgctg ccgtcggctg ctatgtcgac ggccagtcgc
     1201 tcatgtgtca gcgcgtcgga aagcgcggac agcatcgtcg gttcatcttc tactacgagt
     1261 actctcatcg tcgctctcct cctttctcct aaagtcacag gtctccctgc gcgcctcaat
     1321 gctatcagca tagcgttaac ccatcgttaa gtggaggcca gccgattgcg ctccagcaac
     1381 atgagggcac aatagtcata tgaagaacga cagaaggaat gtgaagccca tagtgcctga
     1441 ccccacaggg attcacaatt cgtcatgagc gtgaggaagc cggaagggga gcagcgcacg
     1501 agtcccgtgg caggcgccta tgtgctggtg cggtcgcgcc gccggtccat cgggctggag
     1561 gtccggccgg acgccacgct ggtggtgcgg gcgcccacac ggacgccggt gtggttcgtg
     1621 gagtccctgc tgcgggagaa ggctgggtgg atcgaggaca agctggcaaa ggcgcgcagt
     1681 catgtgtccc tgctgccgcg gcacgacttc ctgacggggg agcgctttcc gtacctgggg
     1741 cgtgagtggc cgttcgcggt cgtcgcgttc cagaagacgc cgctgacgtt cgacgaaaag
     1801 tccgggttca gcctggacat ggctgcattc gaccgaggag aagtggtgtt cgaggaatgg
     1861 taccggtccc gggcacgaga gctccttgct gatcgcgtgc tgcactatgc accgctggtc
     1921 ggtgttgcca ttccccatct gcgcatcacc ggcgctgagc ggcgctgggg gtcgtgcagc
     1981 acgaccggaa ccgtcagctt tgcctggcgt ctcatcatgg ccccgatgga tgtggtcgac
     2041 tacgtcgtcg tgcatgaact ggcacaccgg cgcgagatga accactccgt gaggttctgg
     2101 actctcgtcg catctgtaat gcaggactat gacacgcgca ggcgctggct caaggagcac
     2161 ggggggatgc tgactatctg agcaggtttc gtgaaagcag ctgtcgcgcg cagaccgcat
     2221 gaactcctgt ctctatggcc aatgtggaat acccggggtt gacgatggtc gaaagcaggg
     2281 tagaacttct gttgttcgcg aaatctgctt tcagaagagg tgttgcttca tgaaacgaat
     2341 cacaagtctc ctggtcgcct ttctcctcgt cggcatgctg gttgtcgcag ctggttgcgg
     2401 caaggctgct gcagcccctg cgacgaccga tccagcgtcc acgaccgatc agacacgggc
     2461 cacagaccag actcagtcga ctgatcagaa cgggacgacg tacgaggatc agacgacaga
     2521 cggaacgcag gcgccgggca cggagaccac tccatcaggc gaccagactc aggtcacccc
     2581 tcagggtggc acagtccagt agcacctgtc cttttctcgt gtgaacgcgg atgccccggc
     2641 ttgccccggg gcttcttcaa ttctatcgac gcaggaccca ccagacgtcg cggccggcga
     2701 agccacaacg gcggaacagg tggtccggtt cggaagggtt cttgagctga ccggcgaccg
     2761 tcgtgaagtt gacttgctgt tccaggcgat aaacaagctc ttggacgagc atggtcccca
     2821 gtccaaggct gcggaacgcc ggcaggacct ggatccactc gagcgatgct tcttcagtga
     2881 tcctgtcagt ttctgcgatg ccaagagcca caggacgcct ctccttgcgg tcgatgatcc
     2941 acagccaggc gtgcgggtcg aagacggcac gcttcgtcca ggcggcgatc tcgtcggtac
     3001 ccggcgtagc cgtgtcatag caggcgtgca ggatcgtggc cacgagcggg acctcgcttg
     3061 tcgtgtcgac ctcgcggatg gcgtatccgg ggacagtggc gtgcagaggc agttcgctgc
     3121 cggcgtggat caggcggaag aacgcctcct tgcggtcgaa ccatggtgca agtggagcca
     3181 cgaagtcgtt gtggatccac accgtgggca cgccatcgag cttgacagac gctgccttga
     3241 gcgggtcctg agttcgtgtt ccgtacatga gcagtttccc agcctctcgc aattcgactc
     3301 cggtggtctg ttcgttgtcg gcgatgaacc tgcactcaag tgactgcagg cgcgccacgg
     3361 tcttccacag tggagttggc aggacgcgca gggggtccct gcgat
//
LOCUS       DATMZD010000047         2589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_71924,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000047 DATMZD010000000
VERSION     DATMZD010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71924"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1363
                     /locus_tag="VN478_00970"
     CDS             <1..1363
                     /locus_tag="VN478_00970"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008241 - peptidyl-dipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HWQ21281.1"
                     /translation="RQLTLVHDMMVENQLDKEMLQQLVSKQIALQGITTKFRGTIDGR
                     EVNNKQIEDILRSSIDVELRKKAWLASKQVGAVLEPGLMELIKLRNESAVKLGYPNYY
                     EMSIQLQEFDGDWLKQTLESYRDQTDSLFRRVKDSIDQAVGKRLGVDPSTLMPWHMSD
                     MFFQEAPRTSEVDLGVYFEGKGDHVVEELATATYDAIGLDIRDIIARSDLYERAGKDQ
                     HAFCMHLDNRGDVRVLANLRPNETEMETLLHEMGHACYDKYTDMSLPEVLRQPAHIFT
                     TEAVAMFFGRMARDPKWYKQMVGVDDANLQRLQTDLPKAIRNQMLVATRWITHFALFE
                     RELYRTEKGDSRLWYQGVQNIQYLNAPEDRFEKPDWAAKYHFAMAPVYYHNYLLGEML
                     ASQFDHTLRTKADGKVLTPEGGRWFVDNVFTPGAKYSWNTMIEHATGEKLNPSYLVQQ
                     FNY"
     gene            complement(1435..2118)
                     /locus_tag="VN478_00975"
     CDS             complement(1435..2118)
                     /locus_tag="VN478_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HWQ21282.1"
                     /translation="MQTIPEQQAKERIASVIARHFDAEAQSGRLFINLGVGIPTVVAN
                     YVTNPHVYIQAENGMLGVGCAAPEGQMDHDLINAGRTCVTETPGCSYLDSAASFGMIR
                     GGHVDATVIGAFEVDQTANIANWIIPNGKQLGVGGAMDLVTGAREVVIAMQHTSKNGK
                     AKLVRQCTLPVTGIGEADMVVTEYAVFLFDDGRLVLRELAPEITVDELRAITEAEFEV
                     SPELRTMAV"
     gene            complement(2120..>2589)
                     /locus_tag="VN478_00980"
     CDS             complement(2120..>2589)
                     /locus_tag="VN478_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit A"
                     /protein_id="HWQ21283.1"
                     /translation="LLTNNQIRALIGTYYNWNPDAAVAHNEGRIEVTLVPQGTFAEAI
                     RAAGVGIPAFYTPTAAGTELAAGKETREFDGRACVLEPAIHADFAFIKAYKADELGNL
                     VYYKTGRNFNPVMAMAADTVIAEVDEIVPVGSLDPECIVTPHIYVDMLTMRKG"
BASE COUNT          549 a          817 c          726 g          497 t
ORIGIN      
        1 ccgacaactc actctagtcc acgacatgat ggtcgagaac cagctcgaca aggagatgct
       61 gcagcagctc gtcagcaagc agatcgccct tcaaggcatc acgaccaagt tccgtggtac
      121 catcgacggc agagaggtca acaacaagca gatcgaggac atccttcggt ccagcatcga
      181 cgtcgagctc cgcaagaaag cctggctggc aagcaagcag gtgggcgccg tgctcgaacc
      241 cgggcttatg gagcttatca agctccggaa cgagtccgca gtcaagctgg gctaccccaa
      301 ctactacgag atgtcgatcc agctgcagga attcgacggc gattggctca agcagacgct
      361 ggaaagctac cgcgaccaga cggacagcct cttccgccgt gtcaaggatt ccatcgacca
      421 ggcggttggc aagcggctgg gtgtcgaccc ttccacgctc atgccgtggc acatgagcga
      481 catgttcttc caggaggccc cccgcacctc agaggtcgat ctcggcgtgt acttcgaggg
      541 caagggcgac cacgtcgtcg aggagcttgc gacagcaacc tacgatgcga tcggcctgga
      601 tatccgtgac atcatcgccc ggagcgacct gtacgagcgc gccggcaagg accagcacgc
      661 cttctgcatg cacctggaca acagggggga cgtccgtgtc ctggccaacc tgcgtcccaa
      721 cgaaaccgaa atggagacgc tccttcacga aatgggccac gcctgctacg acaagtacac
      781 tgacatgtcg ctgcccgagg tcctgcgcca acctgcccac atcttcacga ccgaggccgt
      841 cgccatgttc ttcgggcgca tggcccgcga tcccaagtgg tacaagcaga tggttggcgt
      901 cgacgacgcg aatctccaga gactgcagac tgaccttccc aaggcgatcc gcaatcagat
      961 gctcgtcgcg acccggtgga tcacccactt cgcgctcttc gagcgggagc tgtaccgcac
     1021 cgagaagggt gacagccggc tctggtacca gggcgttcag aacatccagt acctcaatgc
     1081 ccccgaggac cgcttcgaga agcccgactg ggcagcaaag taccacttcg ccatggcacc
     1141 ggtctactat cacaactacc tgctgggtga gatgctcgcc tcccagtttg accacacctt
     1201 gcgaacgaag gccgacggca aggtcctcac acccgaaggc ggccggtggt tcgtggacaa
     1261 cgtcttcacg cccggcgcaa agtattcgtg gaacaccatg atcgaacacg cgaccggcga
     1321 gaagctcaac ccgagctacc tggtgcagca gttcaactac tagacaagcg gctacagacg
     1381 tcaaagggcc cggcagacaa cgccgggccc tttcgtttcg cggaggccga gatactagac
     1441 ggccatcgta cgaagttcag gacttacttc aaactccgcc tccgtaatgg ccctgagttc
     1501 atctactgtg atctcgggcg caagttccct gagtacgagt ctgccgtcat cgaagaggaa
     1561 cactgcatac tccgtgacga ccatgtccgc ttcaccaatc ccggtgacag ggagtgtgca
     1621 ctgtctgacc aacttggcct tgccattctt gctggtgtgc tgcatggcaa tgactacttc
     1681 ccgtgcgccc gtgaccaggt ccatcgctcc gcccactcca agctgcttgc cattggggat
     1741 gatccagttg gcaatattgg ccgtctgatc cacctcgaac gctccgatga ccgtcgcgtc
     1801 cacatgccca ccacggatca tgccaaacga tgcagcgcta tccaggtagg agcagccggg
     1861 tgtctcggtg acgcaggtcc tgccggcatt gatgagatca tggtccatct ggccctcggg
     1921 agcggcacag cccacgccga gcatgccgtt ttctgcctgg atatagacgt gcgggttggt
     1981 gacatagttg gccaccacgg tcggaatgcc caccccaagg ttgatgaaca acctgccact
     2041 ctgtgcctcg gcatcaaagt ggcgcgcgat cactgaagct atacgttcct ttgcctgctg
     2101 ttcgggtatg gtctgcattt caacccttcc tcatcgtcag catgtcgacg tatatgtgag
     2161 gcgtgacgat gcactcagga tcgagcgacc ccacaggcac gatctcgtcg acctctgcga
     2221 tgaccgtgtc cgccgccatg gccatgacgg gattgaagtt ccgaccggtc ttgtagtaga
     2281 cgaggttgcc aagttcgtcg gccttgtacg ccttgatgaa agcgaagtca gcatggatgg
     2341 caggctcaag gacacatgca cgaccgtcga actcccgtgt ttccttcccg gcagccagtt
     2401 ccgtccctgc agctgtcggg gtgtagaatg caggaattcc cacccccgca gcacggatag
     2461 cctcggcgaa tgtgccctgc gggaccagtg tcacttcgat cctgccttcg ttgtgggcaa
     2521 ctgccgcgtc aggattccag ttgtagtacg tcccgatcag tgcccgtatc tggttgttgg
     2581 taagcagtc
//
LOCUS       DATMZD010000048         2773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_31641,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000048 DATMZD010000000
VERSION     DATMZD010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31641"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..970)
                     /locus_tag="VN478_00985"
     CDS             complement(<1..970)
                     /locus_tag="VN478_00985"
                     /inference="COORDINATES: protein motif:HMM:NF024486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HWQ21284.1"
                     /translation="MNEELAVHIAAFAAAIEDEVTAEKASERTHRHALVEGTLVSDAG
                     DENFLYFFSTPYEVNLPEESMIQLEVDDRVVQGVYAGGDRDLVSIASSEFLGPQVRAA
                     TMIATPWDLLLKLEERLNGLVDEDDCGRALALLSPPVPGDSLPELNGVFAENRWLDPS
                     QRDALQKVAERDVSFIWGPPGTGKTRTVAYLVRELIERRKSVLLLAHSNAAVDVAALA
                     VVNACRGTPYLIQGAMIRYGIITKRDLLVESDVNPLNILARRNVDAERFVGLQSRRRD
                     LLEHAKREELFDTAEFETVRGDMGKLRHRVDKELKKLVSETQVVATT"
     gene            1098..2099
                     /locus_tag="VN478_00990"
     CDS             1098..2099
                     /locus_tag="VN478_00990"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HWQ21285.1"
                     /translation="MYKPTTKLSAVLTEHPRAATYLASTNPKLKLLGNPVVIGLMAPR
                     TDLRKIAERAGWTLEELEAAVIRSDIEGTPPATSEEAPAKLKEDLKAVLRRLYGGEDP
                     ATCKAQFKELIAKADPLLIATAEAELAREGFSTDQLMEACDVHMDVFRDQLASSRTVV
                     PDDHPLKRFIREQDVIIAWLEQGHALARTLGALSGYAEAERALAELHSLMQHLHEAQS
                     HDARQENPLFPLLERYGVEEPPAIMWAEHNRMKATRDLIDKTLLGAPDALPYAQFAQV
                     LAGAFQHWLETFVQHARKEQEILYNVALDLLSPKDWQDLEQESRELGFFALPGEENA"
     gene            2096..2518
                     /locus_tag="VN478_00995"
     CDS             2096..2518
                     /locus_tag="VN478_00995"
                     /inference="COORDINATES: protein motif:HMM:NF024983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HWQ21286.1"
                     /translation="MSDTGLDLFDGMDLEVLLALFDTLPVDVSFVNADDEVTYFNLPA
                     AGRIFPRTRLDVGRRVQRCHPPQSVDKVQRILDDFRAKRRSTADFWLELGGKFVYIRY
                     FAVYGKDGAYQGTLEVSQDCTTIRALQGEKKLEGDLTD"
BASE COUNT          545 a          881 c          840 g          507 t
ORIGIN      
        1 gcgtcgtcgc gacgacctgc gtctcggaga cgagcttctt gagttccttg tcgacacggt
       61 ggcgcagctt gcccatgtcg cccctgaccg tctcgaactc ggcggtgtcg aagagttcct
      121 cccgcttcgc gtgctcgagc aggtcacgtc tcctggactg aagacccacg aagcgctctg
      181 cgtcgacgtt gcgcctggca aggatgttga gtgggttgac gtcggactcc accagaaggt
      241 cgcgcttcgt gatgatgcca taccggatca tggcaccctg aatgagatac ggtgtccccc
      301 ggcaggcgtt caccacggca agcgccgcga cgtcgaccgc cgcgttgctg tgcgccagca
      361 agaggacgct cttgcggcgc tcgatgagct cgcgcacgag gtaggcgacg gtccgcgtct
      421 tgccggtgcc cggcggaccc cagatgaagc tgacgtcgcg ctcggcgacc ttctgcaatg
      481 cgtcacgctg agacgggtcc agccatctat tctccgcaaa gacgccgttc agttcgggca
      541 ggctatcccc tggaacagga ggactgagga gagcaagggc tcggccgcaa tcatcctcgt
      601 cgaccaaccc attcagccgt tcttccagct tcaggagcag atcccagggt gtggcaatca
      661 tcgtggcggc acgtacctgg ggccccaaaa actcgctgga agcgatcgac accaggtccc
      721 ggtctccgcc cgcgtagacc ccctgcacta ccctgtcgtc cacttcgagc tggatcatgg
      781 actcctcggg caagttgact tcatatggag tactgaagaa gtagaggaag ttctcgtcac
      841 cagcgtccga caccagggtg ccctccacga gagcgtgacg gtgtgtcctc tccgatgcct
      901 tctcggcggt gacttcgtcc tcgattgcag cggcgaacgc cgcgatatgg actgccagtt
      961 cctcgttcat gctcactccc gtcatcacgg cgccgtgtca cgcgagcatt cattataccg
     1021 ccacgcggtt tcctgtatac tgagcaggtg acatgcacga gttcactggg atgcagcgtt
     1081 gtcagtggag gtatctgatg tacaagccaa cgacgaagct ctccgctgtc ctgaccgaac
     1141 acccgcgggc agcaacatac ctggcatcga ccaacccgaa gctgaagctc ctcggcaacc
     1201 cggtcgtcat cggcctcatg gccccccgaa ccgacctccg gaagatcgcc gaacgggccg
     1261 gctggaccct ggaggagctg gaagcggccg taatccgcag tgacattgag gggactccgc
     1321 cggcaacgtc ggaagaggct ccagccaagc tcaaggagga cctcaaagct gttctccgga
     1381 gactgtacgg aggtgaggac cccgccacgt gcaaggcgca gttcaaggag ttgatcgcca
     1441 aggcggaccc ccttctcatc gcgacagccg aggcggaact ggcacgcgaa ggcttctcca
     1501 ccgatcagct catggaggca tgcgacgtcc acatggacgt cttccgtgac cagctggcaa
     1561 gctcacgcac tgtcgttccg gatgatcatc cgctgaagcg attcatccgc gagcaggacg
     1621 tcatcatcgc ctggctcgag cagggacacg ccctcgccag gactcttggc gcactgagcg
     1681 gatacgctga ggcggagagg gctctcgctg aactccacag ccttatgcag catctgcatg
     1741 aggctcagag ccacgatgcc cggcaggaga acccactatt ccctctgctg gaacgatacg
     1801 gcgtcgaaga gcctcccgcc atcatgtggg ccgagcacaa ccgcatgaag gcgacacgcg
     1861 acctcatcga caagacgctc ctgggagcgc ccgacgctct gccatacgcg cagtttgcac
     1921 aagtgctggc gggagccttt cagcattggc tcgagacctt cgtccagcac gcaaggaagg
     1981 aacaggagat cctttacaac gtggcacttg acctgctgtc cccaaaggac tggcaggatc
     2041 ttgagcagga atcccgggaa ctggggttct tcgcactccc aggggaggag aatgcatgag
     2101 cgataccggt ctcgatctgt ttgacggcat ggacctcgag gtcctgcttg ctctcttcga
     2161 tacgctgccg gtcgacgtct cgtttgtgaa tgcagacgac gaggtgacct acttcaatct
     2221 gccggctgcc gggcgcatct tcccgcgcac ccggctggat gtcgggcgca gggtccagcg
     2281 atgccaccca ccccagagcg tcgacaaggt ccagcggatc ctggacgact tccgcgcgaa
     2341 gagacgttcc acggcggact tctggctgga actgggcgga aagttcgtct atatccgcta
     2401 cttcgccgtg tacggcaagg acggcgccta tcaggggacc ctcgaggtgt cgcaggactg
     2461 taccactatc cgtgcacttc agggtgagaa gaaactcgag ggagatttga ccgactgagg
     2521 caggctggac agctggcata ccaagcgccc ccgctcacgc gggggcgctt acgtttcagg
     2581 cgatggcctc tgaggaccgc cgattcaacg agggccggtc actgtgcggg gagatgtgcg
     2641 tccagccaat cagcggcgaa ggcgagcact tgctccttcg ccccgtccgg ctcgtggtgg
     2701 atgaagtggt acagaccagg ccacagcttg agcgtcacgt caccctgaat gcccgctgca
     2761 agacgctttg acc
//
LOCUS       DATMZD010000049         4325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_31653,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000049 DATMZD010000000
VERSION     DATMZD010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4325
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31653"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="VN478_01000"
     CDS             <1..352
                     /locus_tag="VN478_01000"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HWQ21287.1"
                     /translation="LDPLEWAEMQRHSEYGEKIIKPVHGFGEVAEVVRHHHEKMDGSG
                     YPDHLKGDQVSRFVRIVGVADSYEALTSDRPYRRAFTRDQALEILHKETDEGHWDPQM
                     VAALSGSLDDLDNL"
     gene            389..646
                     /locus_tag="VN478_01005"
     CDS             389..646
                     /locus_tag="VN478_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21288.1"
                     /translation="MNVEVQAILFPYDGSRTSTVIKEFESALEAAGVRCVVGNMAVSC
                     YGEMNTVLSAVSSAYQAIAAGDRLSVSLVIANDMPQIGRKT"
     gene            688..1485
                     /locus_tag="VN478_01010"
     CDS             688..1485
                     /locus_tag="VN478_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21289.1"
                     /translation="MSVTGEECALHCNHCDAHYLQHMVPVTAARRVLESGEYSSILLS
                     GGSDRSGRLPLMEHLAFIAWVHEQGIRINAHVGLQSDEDVKALSPYFDRVSLDYVWDE
                     GTIHDVYHLQRSGVDYLHTTQAWVDAFAAAGGPDALISASQRVNLHLTIGLKGGAIVG
                     EYSAIDSLVEFQPASLVFLVLIPTEGTVYGTVPVVPIADVESVFAYARLKLPSTDLVL
                     GCMHPKAVGYGEQLAHLAAQYRFRGVVGERAEDECVEQVEECCVFYR"
     gene            1522..2547
                     /locus_tag="VN478_01015"
     CDS             1522..2547
                     /locus_tag="VN478_01015"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWQ21290.1"
                     /translation="MRASIGTLAVLGLRQIRMDAVPTTAYLMTSGRCSGDCAYCGQGQ
                     GAVGDHDRLSRITWPEVSEDQLIEAFVSHPGAFQRVCFQTTASRGVLRQVLALAPRIQ
                     KASNTPISVSYRVTTREEADQLFGAGIQRIGVAIDCCSQRLYPQLRGGSLFDSVSLVK
                     QLAALYPGRISTHLIVGLGESEMEAAELMLDLHRSDVLVSLFAFTPVRGTPMEHGSPP
                     ALVSYRKLQLLLGLLDWQKEEQFSVVYDAGSIHGFSLDEAQIRSFLQRHFVFVTHGCP
                     GCNRPFYTEAPGGTMFNFPSVPTGAGRREVDEFIDELKSEDFDFGSKRGFCETGIPSR
                     DARQHGA"
     gene            complement(2662..3546)
                     /locus_tag="VN478_01020"
     CDS             complement(2662..3546)
                     /locus_tag="VN478_01020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HWQ21291.1"
                     /translation="MNSNKRLGRGLDALFANEPPQSAAPGDSGVRTLRPDQIVPSPLQ
                     PRRRFDDAALATLGESIRKHGILEPIIVRPRDGRFEIVAGERRFQAAVRVGLEQLPVT
                     VRDLDDRTTMEIALSENLEREDLSPIEVAVSFSDYLTKFGTTQDDLAQRLGKDRSTIA
                     NLIRLLDLPEIARTALDNRTITAGHARALLPIKEQRQLTAVLAEVIKKQLSVRQTEAL
                     VRRLLTKKPSAGIIHDDALRNIQETMQKLLATHVNIRGRVSKGVIEISYFNQEDLLRI
                     LGILNSQQEGGDDAAPAN"
     gene            complement(3533..4297)
                     /locus_tag="VN478_01025"
     CDS             complement(3533..4297)
                     /locus_tag="VN478_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HWQ21292.1"
                     /translation="MRIIAIANQKGGVGKTTTALSLAASLAELNHRVLLVDFDPQANA
                     TTGLGINAAELASNIYTCLLSDDPIQNAVQPTREHNLSLVPSSINLAGAELELAGMDR
                     REYALQNALAALDGSWDYIIIDSPPSLGLLTLNTLTAAHEVIVPIQSEYYALEGLTHL
                     LRTISLVKQRLNPNLSLLGILVTMFDKRTILSAQVKDELVKHFGDTVFTAVIPRSVRM
                     SEAPSYGKTILEYDTLSTGAEAYRQLAKEVESREQQ"
BASE COUNT          791 a         1249 c         1391 g          894 t
ORIGIN      
        1 gctcgatcca ctggagtggg ccgagatgca gcgccacagc gaatacgggg agaagatcat
       61 caagcctgtc cacggattcg gcgaagtggc cgaggtcgtg cggcatcatc atgaaaagat
      121 ggacggttcg gggtatcctg atcatctgaa gggtgaccag gtctctcggt tcgtgcggat
      181 cgtcggagtg gccgacagct atgaggctct tacgagcgac cggccgtacc gtcgtgcatt
      241 tacgcgcgac caggctctgg agatccttca caaggagact gacgagggac actgggatcc
      301 acagatggtc gctgcactgt ccggttctct cgacgatctc gacaatctgt aagtcgcccc
      361 tgcgggggct ggaaaggaag gtcatttcgt gaacgttgag gtccaggcaa ttctgtttcc
      421 gtacgacgga tcgagaacgt ctaccgtcat caaggaattc gagagcgcac ttgaggcagc
      481 cggagtccga tgtgtggtcg gcaatatggc cgtgagctgc tacggcgaga tgaacacggt
      541 tctctcggct gtcagcagcg catatcaggc catcgcggcg ggggacaggc tcagcgtttc
      601 ccttgtgatt gccaacgaca tgccccagat aggacgcaag acctgaactc tatggtcttc
      661 agctgcatcc ccagacggac actctccgtg tccgtcactg gcgaggagtg tgccctgcac
      721 tgcaaccact gcgacgcaca ctaccttcag cacatggttc ctgtgaccgc agcccgtcgc
      781 gtgctcgagt ccggagagta ttcaagcatc ctgctgtccg gcggaagcga ccgcagcggt
      841 cggctgcctc tcatggaaca tctggcgttc atcgcgtggg tgcatgaaca gggcatacgc
      901 atcaacgctc acgtcgggct gcaatcggac gaagatgtaa aggcactgtc accgtacttc
      961 gacagagtct cgctggacta cgtgtgggac gaggggacga tccatgacgt ctaccacctt
     1021 cagcggtccg gagtggacta cctgcacacg actcaggcct gggtggacgc gtttgcggcc
     1081 gcgggcgggc ccgacgccct gatatcggcg agccagaggg tcaatctcca cctcacgatc
     1141 ggtctcaagg gtggcgcaat cgtgggtgaa tactccgcga tcgactcact ggtcgagttc
     1201 cagccggcgt cgctcgtgtt tctcgtcctc attcccacag aggggacagt ctacggcaca
     1261 gtccctgtcg tgcctattgc ggatgtcgag tcagtgtttg catatgcgag actgaagctg
     1321 ccgtcgactg accttgtgct cggctgcatg catccaaaag cggtcggcta cggcgagcag
     1381 ctggcacatc ttgccgcgca gtatcgattc aggggcgtcg tcggcgagcg agccgaggac
     1441 gagtgcgtgg aacaggtgga ggagtgctgt gtgttctacc ggtgaacgct tctcccgcgg
     1501 ccgctggacg aactgacggt ggtgcgcgca agcataggaa cgctggcagt cctggggctt
     1561 cggcagatcc gcatggatgc tgtgccgacc acggcgtatc tgatgaccag tggcaggtgt
     1621 tcgggagact gtgcctactg tgggcaggga cagggcgccg tcggcgatca cgaccgcctc
     1681 tcgcgtatca cctggcctga ggtcagtgaa gaccagctga tcgaggcgtt tgtctcccac
     1741 cctggcgcat tccagagggt ctgcttccag acgacggctt ctcgcggggt gctcaggcag
     1801 gtacttgcac tggcaccacg gatccagaag gccagcaaca cccccatttc tgtttcatac
     1861 cgcgtgacga cgcgtgagga ggcggaccag ctgttcggcg cagggatcca gcgtatcggc
     1921 gtcgccatcg actgctgctc gcagcgcctc tatccgcagc ttcggggcgg gtccctgttc
     1981 gattccgtgt cactggtgaa gcagttggcc gcgctgtacc ccggtcgcat ctcgacacac
     2041 ttgatcgtcg gattgggcga gagcgagatg gaggctgccg agctgatgct cgacctgcac
     2101 aggtcagacg tcctggtatc gctttttgcg ttcacgccgg ttcgcggtac gcccatggag
     2161 cacggttctc cgcctgctct tgtgtcctac cggaagctcc agttgctgct cggccttctg
     2221 gactggcaaa aggaagagca gttctccgtc gtctatgacg ccgggagcat ccacggcttc
     2281 agtctggacg aagcgcagat caggtcgttc ctgcagaggc acttcgtgtt tgtgacccac
     2341 ggctgtcccg gatgcaaccg ccccttctat actgaggcac ctggcggcac gatgttcaac
     2401 ttcccgagtg tgccgaccgg agcggggcgc cgggaagtcg acgagttcat cgatgagctg
     2461 aagtctgagg acttcgattt cggctcaaaa cggggtttct gcgagacggg gattccgtca
     2521 cgtgatgcgc ggcaacacgg cgcttgagag cgagtgggtc cagactcgcg aatcgacagt
     2581 ggctggacgc tgaggagact gaggagatgc gagagccccg acgctacgtc ggggctctgt
     2641 cacagacagt agtcgcgagg cctagttcgc tggagctgcg tcgtccccgc cctcctgctg
     2701 agaattgagg atgcccagga ttcgcagaag gtcttcctgg ttgaagtagc tgatctcaat
     2761 gacgcctttg ctgacccgac cccgtatgtt gacatgtgtt gccagcagtt tctgcatggt
     2821 ctcctggatg ttgcgcagcg cgtcgtcgtg tatgatgccg gcgctgggct tcttcgtcag
     2881 cagacgtcgc acaagcgctt ccgtctggcg caccgacaac tgcttcttga tgacttcggc
     2941 cagcactgcg gtcaactgtc tctgctcctt gatcggcagg agtgctcgag catgtcctgc
     3001 ggtgatggtc cggttgtcca gggcggtgcg tgcgatctcg ggaaggtcaa gcagacggat
     3061 caggttggca atcgtactgc ggtccttacc cagccgctgc gcgaggtcat cctgcgtcgt
     3121 accgaacttc gtgaggtagt cgctgaagga gactgcgacc tcgattggag acaagtcctc
     3181 acgttcgagg ttctccgaca gagcgatctc catggtcgtt cgatcgtcga ggtcccggac
     3241 ggtaacggga agctgttcca gtcctacccg cacagcagcc tggaagcggc gttctccagc
     3301 gacgatctca aacctgccat cacgcgggcg cacgatgatt ggctcaagga taccgtgttt
     3361 ccgtatgctc tcgcctagcg tcgcgagtgc tgcatcgtcg aaccggcggc gcggctggag
     3421 tggcgacggg acgatctggt caggacgaag agtgcgaacg ccgctgtcgc ctggcgctgc
     3481 ggactgagga ggttcgttgg cgaagagggc atccagccct cgtccgagac gcttattgct
     3541 gttcacggga ttctacctcc ttggcaagct gccggtacgc ctccgcaccg gtggaaagcg
     3601 tgtcgtactc caggatggtc ttgccgtagc tgggcgcctc gctcatgcgt acgctcctgg
     3661 ggatgacggc ggtgaagacg gtatctccga agtgcttgac gagctcatcc ttgacctgtg
     3721 cagacaggat ggtccttttg tcgaacatcg tcaccaggat tcccagcagc gagagattcg
     3781 ggttcaggcg ctgcttgaca aggctgatcg tacgcagcag gtgtgtcagc ccctccagcg
     3841 cgtagtactc actctgaatg gggacgatga cctcgtgcgc ggccgtcagt gtattgaggg
     3901 tcaggagccc cagcgacgga gggctgtcga tgatgatgta gtcccaggac ccgtccaggg
     3961 cggcaagcgc attctgcagg gcatactcgc ggcggtccat gccggccagt tccagttcgg
     4021 cgcccgccag gttgatcgac gaagggacca gtgagaggtt gtgctcgcgg gttggctgga
     4081 ctgcgttctg gattggatcg tcggacagca gacaagtgta aatgttcgaa gcgagctccg
     4141 ccgcattgat tccgagcccg gtcgtcgcgt ttgcctgggg atcgaagtcg acaagaagga
     4201 cacgatgatt gagctcggcg agtgatgcgg cgagcgacag cgcggtggtc gtcttgccca
     4261 ccccaccctt ctgattcgca atggcgatga ttctcacgac ccgatcctcc cggacgaccg
     4321 accgc
//
LOCUS       DATMZD010000050         2058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_142035,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000050 DATMZD010000000
VERSION     DATMZD010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142035"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..924)
                     /locus_tag="VN478_01030"
     CDS             complement(<1..924)
                     /locus_tag="VN478_01030"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWQ21293.1"
                     /translation="MERKVLVVDDDVESLRLYGDMLRRAGYAAVTASTGWEGIDLAVK
                     EQPGLILLDLRLPDITGLTVLETLRTALSTKNIPIIVVSAISKDDDVMKAMQMGAFDY
                     VVKPITYDDMIRRVELVFFSRERQGEGDQAPVLIVEDSAVDGAALSEQLNTWGYTVVW
                     VRTGHEGIEQAATGNFDVILLDGLLPDINGFEVVKEIRATERLGTTPVIMLTVLSGLV
                     DKLKGFESGVDEFLNKPVSWEELRVRMRSLTRLRGMTKQRFALSEVVGVITNAIELAE
                     ATEPRHNQKVASISVILGKRMGVQGHGLWVLE"
     gene            1104..1784
                     /locus_tag="VN478_01035"
     CDS             1104..1784
                     /locus_tag="VN478_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21294.1"
                     /translation="MRTFTEVDSASTVHEDTRGRAASDVRSTLIVHSASGHSGKTTIC
                     RSLVRDLPFDVYIKLSRHSLHLVERSLAAGTLPATGGDTGRLSQSERSPALQPLRDII
                     LLDGPHDETESAVVRIFQQWPVETRILIEGNCSSVPERSRRAYVLRCPLLQSAKSNMS
                     SMSSLADLLVINRFPGCTPAEESALLALLRDWNPRANQVSGSVEDAGFLETVEAAVLD
                     LLPALRNA"
     gene            1851..2033
                     /locus_tag="VN478_01040"
     CDS             1851..2033
                     /locus_tag="VN478_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21295.1"
                     /translation="MEEIDQEVRDELLKAATDGFIDCGKAIAIARKLNIDPRVVGKAC
                     DLLHIRVRSCSLGLFD"
BASE COUNT          401 a          671 c          571 g          415 t
ORIGIN      
        1 ctccaggacc catagcccgt gtccctggac gcccatcctc ttccccagga tgacgctgat
       61 actcgcgacc ttctggttgt ggcgcggctc tgtggcctcg gccagttcga tcgcgttcgt
      121 aatgacgccc acaacctccg acagggcgaa gcgctgcttg gtcattcccc ggaggcgcgt
      181 cagggaacgc attcgtacgc gcagttcctc ccaggatacg ggcttgttga gaaactcgtc
      241 gacaccgctc tcaaatccct tgagcttgtc cacgagccct gacagcacgg tcagcatgat
      301 aaccggggtc gtgcccagcc gctctgtcgc ccggatctcc ttcacgactt caaacccgtt
      361 gatgtcggga aggagcccgt ccagaaggat gacgtcgaag ttgccggtag cggcctgctc
      421 gatgccttcg tggcccgtcc ggacccaaac gacggtgtat ccccaggtat tcagctgttc
      481 cgacagtgcc gccccgtcga ccgcactgtc ctcgacgatg agcacgggag cctgatctcc
      541 ctctccctga cgttcgcgtg agaagaagac gagttcgacc ctgcggatca tgtcatcgta
      601 ggtaatgggt ttgacgacgt agtcgaacgc tcccatctgc atcgccttca tgacgtcgtc
      661 gtccttgctg atcgcagaca cgacgatgat agggatgttc ttggtcgaca gcgccgtgcg
      721 gagtgtctcg agaacggtaa ggccggtgat atccgggagg cgaaggtcca gcaggatcag
      781 gccgggctgt tccttgacgg cgaggtcgat gccttcccag ccggtgcttg cggtgacggc
      841 tgcatagccg gctcgccgca gcatgtcgcc gtagagacgc aggctttcga catcatcgtc
      901 gactaccagg acctttcgtt ccatcaacac acccccggct ctctgtcaac tacccgctcc
      961 accagaccag cagtgacaac gcggtcaccg tactcccacc agcgcactaa ttgagtatta
     1021 tctatgcaac cagacaaaaa gcaattcgca caacacggcc tgcgaatgcg gcaaacgcag
     1081 gcaactcgac ctcggaggca ccaatgcgca ccttcaccga agtagattcg gcaagcacag
     1141 tccacgaaga cacacggggc agagcggcct ctgatgtacg ttccaccctc attgtccatt
     1201 cggcctcggg gcactccggc aagaccacga tctgccgctc tctcgtgcgt gaccttccat
     1261 ttgacgtcta catcaaactg tcacgtcaca gcctgcatct cgtcgaacgt tctctggcgg
     1321 cagggacgct gcccgccaca ggaggggata ccggtcgcct cagtcagtcg gagcgctctc
     1381 cagctttgca gccgctgcgt gacatcatcc ttcttgacgg gccccacgac gagacggaga
     1441 gtgccgtcgt caggatattc cagcagtggc ccgttgagac tcgcatcctc atagagggca
     1501 actgctcgtc ggtcccggaa aggtcacgcc gtgcgtacgt cttgcgctgt cctctcctcc
     1561 agagcgccaa gtccaacatg agctcgatgt cttccctggc cgacctgctc gtcataaacc
     1621 ggttccccgg atgcacgccc gcggaggaat ccgctctcct cgcactcctc agagactgga
     1681 atcctcgtgc aaaccaggtc tcagggtccg tggaggacgc cggattcctc gagacggtcg
     1741 aagcagctgt cctcgacctt cttccagctc tccgcaatgc ttgaaaatgc gctccagcat
     1801 ggcacactgt gatgtctgca ttccgcagga tcaggaacca ggaggctccc atggaagaga
     1861 tcgatcagga agtccgcgac gagttgctca aggcagcgac tgacgggttc atcgactgtg
     1921 gaaaggcaat cgccattgcg cgcaagctga acatcgatcc cagggtcgtg ggcaaggcct
     1981 gcgacctgct gcacatacgg gtccgttcct gttctctggg cctgttcgac tagctggtgc
     2041 cggcgctctt cattctcg
//
LOCUS       DATMZD010000051         5798 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_122220,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000051 DATMZD010000000
VERSION     DATMZD010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5798
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122220"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            344..1900
                     /locus_tag="VN478_01045"
     CDS             344..1900
                     /locus_tag="VN478_01045"
                     /inference="COORDINATES: protein motif:HMM:NF024977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWQ21296.1"
                     /translation="MKEDAGTDISSDDVFSEMAAETVEPRASALIESLRAFGYTTKTA
                     VADLIDNSITAGARSVHVSFDWSGADSSIAVSDDGSGMDIDTLIEAMRLGTLGPSVVR
                     RPDDLGRFGLGLKTASFSQCRRVTVASQVESTKPVLRAWDLDHVVATNRWELLKTPTR
                     GAEAVISRSLAVGHGTCVVWEKLDQILSTTGTDNRQGYDRFLVLAEQVKEHLAMVFHR
                     FLEGKDLSLEVNGNRIEPWDPYMAGHPGTQHLCSEALDFMGSTIHVDAYVLPHESRLT
                     EEAHRRGGGPGGWNAQQGFYVYRGHRLLVAGDWLGLGTADGTTSYRKEEHYKLARIMV
                     DIPTVLDKYWGIDVKKSTARPPADVRQDFQRIATATRSHAVDVYRHRGKVIQRGVAPE
                     DCVWLESVVSGRSSFVINRQHPFVGILRDESPHVRQITDALLELVEGTYPTKLVLLRN
                     SEQQNVKEPLEELLTQSKAASLGRVLHESLMTRLGITSEQAWERLERLEPFNSQPEAL
                     LAAKRQEKGG"
     gene            1902..4751
                     /locus_tag="VN478_01050"
     CDS             1902..4751
                     /locus_tag="VN478_01050"
                     /inference="COORDINATES: protein motif:HMM:NF022063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z1 domain-containing protein"
                     /protein_id="HWQ21297.1"
                     /translation="MEQRQQIKQLVMSWIRQNSDRSLTDDEIIGVIKWASDAPGGVAE
                     HSEQQIVDVDSILREVKAECAIWVESGLQHLRVPHRDWLPGRRSGINWYYWKSYRTYL
                     EDTGAMAPASLDNMDELTGEILNEMGDPSESGDWDRRGMVVGEVQSGKTANYIGLIDK
                     ALDAGYKFIIILAGTQDDLRSQTQQRVDLGVLGVDSCQGGAEMGAANIGVGLRRPLGR
                     EHVSVYPMTDAKLDGDFNQKKFTNVVIGGAPLLLVVKKNGHILKRIHRWVLSQTKKQS
                     DAGGGVIHGVPLLLVDDEADSASINTKPLAIGKGESAQEENDPTVINRSIRQILNSFG
                     QRSYVGYTATPFANIFIYPGDDAGDRRKYGDDLFPRHFIFNLPTPSNYVGPSELFGLA
                     DSRMSIKHPEGLPLVKYVTDSEAYIPVPHKKDLVVEALPPSLVEAIAAFVLVGAVRRV
                     RGQSSRHNSMLIHVTHYVKVQAEVAALVKAETARLRRTLEFGTGKPYEELLNKLKAMY
                     RSDLQPTARAVADCVAQALPALPSWERVQEELYAAAAKIDVRTVNGEAKDALDYDLYD
                     DTGLSVIVIGGNKLSRGLTLRDLSVSYYLRSTTMYDTLLQMGRWFGYKDGFLDLCRIY
                     TTREIAAFYQWIAGADLELRDEFTAMAEQNATPEEYGLMVRKHPTTLQVTAAGKMRAA
                     RETQVSYAGTLVETFSFRKQAEEQEANFHLIESWIAGLPHVDQDKRGGLSWYHVGHDR
                     VTQLLASFVVDPVFNTMNPASMTEYIRSQAEKNELLDWTVFVISVKTSTSKVARIAGY
                     DGIGLPLRTGVEGSDGRTYLLSKNHLISRADEYKDLSKAELDQALTATQIAWEERMAP
                     GEPSKKAPDQPSPPFVRAARPAQRGLLLIYPLDPSVVSGGWDGDLASSTPIVGLAISF
                     PASKTALPIKYMASYRLWAERLGEEEIDDEPDDLV"
     gene            4729..5742
                     /locus_tag="VN478_01055"
     CDS             4729..5742
                     /locus_tag="VN478_01055"
                     /inference="COORDINATES: protein motif:HMM:NF025748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK motif protein"
                     /protein_id="HWQ21298.1"
                     /translation="MNQTIWSELELKASRGGPSTGLARMLVHPEAPCEMYVAIEKPSM
                     DRVFMVLLGSDEAISSRLPDLRGIRVDYECWTADACERRALVLRARNGSQNDLFATIA
                     EDLADFAAVAPSEQVAVERIAERLQTWQRFLLKRTDTGLSPEACRGLFGELRILDWLL
                     DSHTLGPRVAVESWLGPTGAPQDLAVGRCAIEVKTTAAKQEQRLRISSEKQLDWALVP
                     HLFLAHVSVVEAIDEGISLDEMVEKVRQRLGDNPGRSLFETRLLEAGYSDEDRERYEQ
                     PHFVVSNTLMYEVREGFPCITAKSVPAGVGDLTYSIGVGSCQAFVVAPGAVLGCIRGA
                     AAC"
BASE COUNT         1355 a         1432 c         1785 g         1226 t
ORIGIN      
        1 gtcctcgctg tgtccgcatt tatgccggca cagcgaggtt gtttctctga aacggtcacc
       61 atgattgtag acggtgcccg cacaggaaat tgcaggcagc ccgaacctgg gcttggagcc
      121 cacgattcga cgccgcacca gggagtacct ggtgcggttt gtcattgttg gccgcactac
      181 ttaggctggg tgcctcatcg catatgtcgt tccccgttct tggtccggca cgacagaagt
      241 gccagcgcag atgtatcgcg gcaatgactc attcccagac tttggattat agtatgaatg
      301 agtcagtgat tgactcggga atcagtataa gggtgggcga gccgtgaaag aggatgcagg
      361 aacagacatt agcagtgacg acgtgttttc agaaatggcg gcggagactg tagaaccaag
      421 ggcttcggcg ctaattgagt cgctgcgcgc gtttgggtac acgaccaaga ccgccgttgc
      481 agacctgatc gacaacagta tcacagcggg ggcacggtcg gtccatgtat cgtttgactg
      541 gtcaggcgct gactcttcca tagctgtttc agatgatgga tcgggcatgg acatcgacac
      601 gctcatagag gcaatgagac ttgggacttt ggggccgagc gttgttcgaa gaccagacga
      661 tctcggccgc ttcggcctag gactaaagac ggcttcattc tcgcagtgca gacgcgtgac
      721 cgtggcaagt caagttgaat caacgaagcc cgtacttagg gcttgggatc ttgatcatgt
      781 cgtcgcaacc aacaggtggg agctactgaa gacgccaaca cggggtgctg aagccgtaat
      841 cagtcgttcg ctggcagtcg gacacgggac gtgtgtcgtg tgggagaagc ttgaccagat
      901 cctttcgacg actgggacag acaataggca aggatatgac aggttcttgg tgctagccga
      961 gcaggtgaaa gagcatctgg caatggtctt tcacagattt ctggaaggca aggacctgtc
     1021 tctggaggtt aacggcaatc ggattgaacc ttgggacccc tacatggcag gtcatccggg
     1081 aacacagcat ctctgttcag aggccttgga tttcatggga tcaactattc atgtcgacgc
     1141 ctatgtgctg ccgcatgaat cccggctcac ggaggaagct catcgaagag gcggcgggcc
     1201 tggtggatgg aatgctcaac agggtttcta cgtctacaga ggacatcgac tcctcgtggc
     1261 aggagactgg cttggtttgg gcacagcaga tggcacgaca tcgtacagaa aggaagagca
     1321 ctacaaactt gccaggatca tggtagacat accgaccgta ctcgacaagt actggggaat
     1381 cgacgtaaag aagtccactg cacgcccgcc ggcagacgtg cgtcaggatt tccagcggat
     1441 tgccacggcg acccgtagtc atgccgtgga cgtctaccgg cacagaggca aagtgataca
     1501 gagaggagtg gccccggaag actgtgtatg gctcgagagt gttgtttctg gaagatcaag
     1561 cttcgtcatc aatcgccaac atccgtttgt ggggattctt cgagatgaga gcccacatgt
     1621 acggcagatc actgatgctc tgctcgagct agtcgagggg acatatccta cgaaactggt
     1681 cttgttacgc aattcagagc agcaaaacgt caaggaaccg ctcgaagagc tgctgacaca
     1741 gtcgaaggct gcatcattag gccgggtgct gcatgagtca ctaatgacaa ggttagggat
     1801 cacctcagaa caggcttggg aacgtttgga gcgtttggag ccattcaact cgcaaccgga
     1861 ggcactcttg gcagccaagc gacaggaaaa gggaggctag tgtggagcaa cgacaacaga
     1921 tcaagcaact tgtcatgtct tggattcggc agaattcaga tcgctcactg actgacgatg
     1981 agatcatagg ggtgataaag tgggcgtcag atgctccggg tggcgttgca gaacactctg
     2041 aacaacagat cgtcgatgtc gactcgattc tgcgggaggt gaaggccgag tgcgcaatat
     2101 gggtcgagtc tggtttgcag catctcagag tacctcatcg cgactggctc ccagggaggc
     2161 gtagtggcat caactggtac tactggaaga gttaccgaac ctatctagaa gacacgggag
     2221 cgatggctcc agcctcactt gacaacatgg atgagctgac gggtgagata ctgaacgaaa
     2281 tgggcgatcc gagtgagagt ggtgactggg atcgcagagg catggtagta ggagaagtgc
     2341 agtccggaaa gactgcaaac tacattgggc tcattgacaa ggcgctggac gctgggtaca
     2401 agttcatcat catccttgcc gggacgcagg atgaccttcg tagccagacc cagcaacgag
     2461 tggacttggg cgtgctggga gtggactctt gtcaaggtgg cgctgaaatg ggagccgcca
     2521 atattggcgt cgggctgcga cggccactgg gcagagaaca cgtgtctgtc tatcccatga
     2581 ccgacgctaa actggatggt gacttcaacc agaagaagtt cacgaatgtc gtgatcggag
     2641 gagcgccctt actgttggtc gtcaagaaga acggacacat actcaagaga atccatcgat
     2701 gggtactctc tcagacgaag aagcaatctg atgcaggtgg aggagtgatt catggtgttc
     2761 cgttgcttct cgtcgacgac gaggcagata gtgcgtctat aaacaccaag ccactagcta
     2821 tcgggaaggg cgagtctgct caggaggaaa acgatcccac ggtgatcaac cggagcattc
     2881 gtcagattct gaattccttt ggacagagat catacgttgg ctatactgcg acaccatttg
     2941 ccaatatctt catatacccc ggcgatgatg cgggcgatcg gcggaagtac ggagatgacc
     3001 tcttcccccg ccacttcata ttcaacctcc cgacgccatc aaactacgtt ggccccagtg
     3061 agttgttcgg cttggcagac tcgcgcatga gtattaagca ccctgagggg ctgcctctgg
     3121 tgaagtacgt gactgattcc gaagcataca tccctgtgcc gcacaagaag gacttggtgg
     3181 tcgaggctct tcccccgtca cttgtggagg ctattgccgc gtttgtattg gtcggggcgg
     3241 tgcggcgcgt gcggggacag agcagtagac ataactccat gctgatccac gtgacgcact
     3301 atgtgaaggt tcaggcagag gtggccgcac tcgtgaaggc tgagacggca cgtcttcggc
     3361 gtactctgga gtttggcacc gggaaaccct atgaggagct cttgaacaag ctgaaggcca
     3421 tgtatcgcag cgacctgcag ccgacggcac gtgccgtggc tgactgcgtt gctcaggcgc
     3481 tgcccgctct gccaagctgg gaacgcgttc aggaagaact ctacgcggcg gctgcgaaga
     3541 tcgacgtgcg tacagtgaac ggtgaggcca aagatgccct cgactatgac ctctatgacg
     3601 acacgggtct gtcggtgatc gtgataggcg gaaacaagct gtcgagagga ctgactctga
     3661 gggatctttc ggttagctac taccttcgtt ccactacaat gtatgacacg cttctgcaaa
     3721 tgggtcgctg gttcggctac aaggatggct tcctggacct gtgccgtatc tacactacgc
     3781 gggagatagc cgccttctac cagtggatag ccggtgcaga cttggagctc cgggacgaat
     3841 tcacggctat ggccgagcag aatgccacgc ccgaagaata tggacttatg gtacgcaagc
     3901 atccaacgac cctgcaggtc actgcagcgg gaaagatgcg tgcggcccgc gagacgcaag
     3961 tgtcatatgc aggaaccttg gttgagactt tctccttccg caagcaggct gaggaacagg
     4021 aagcgaactt ccatttgatc gaatcgtgga tagccgggtt gccacacgtt gaccaagaca
     4081 agcgaggggg gctcagctgg tatcacgtcg gtcatgacag ggtgacccag ctgcttgcga
     4141 gtttcgtggt cgacccggtg ttcaatacta tgaaccctgc gtctatgacg gagtacatcc
     4201 gcagtcaggc ggagaagaat gagcttcttg actggactgt cttcgtgata tcggtcaaga
     4261 cgtcgacttc gaaagtcgcg agaatagctg gttacgacgg aattggtctc ccgctcagga
     4321 cgggcgtcga gggctccgat ggacgtacat accttctgtc aaagaaccat ctgattagca
     4381 gggcagacga atataaggat ctttcgaaag cggaattgga tcaggctctg accgctacac
     4441 agatagcctg ggaagaacgc atggcaccgg gcgaaccatc gaagaaggca ccagatcagc
     4501 caagtccacc cttcgtgcgt gcagcacgac ctgctcagcg aggtcttctg ttgatctatc
     4561 cgctagatcc ctccgtggtt tcggggggat gggatgggga cttggcgtcg tctacgccga
     4621 tcgtgggact cgccattagt ttccctgcat ccaagacagc gctgccgatc aagtatatgg
     4681 cgagctaccg tctgtgggct gaacgtctcg gagaagagga gattgatgat gaaccagacg
     4741 atctggtctg aattggaact gaaggcgtca cgaggaggcc catcgacagg actggcacgc
     4801 atgctcgttc acccagaggc cccttgcgag atgtacgtcg ccatcgagaa gccatcgatg
     4861 gatcgcgtct ttatggtcct ccttggctct gacgaggcaa tttcaagtcg gttgcctgac
     4921 ttgagaggca tcagggtgga ctacgaatgc tggactgcag atgcgtgcga gcgaagagcg
     4981 ctggtgttaa gggcaagaaa cgggtcgcaa aacgatctct ttgcgacaat cgccgaagac
     5041 ctggcagact tcgccgctgt ggctccgtct gagcaggtgg ccgtcgagag aattgcagag
     5101 agacttcaga catggcaacg gttccttctg aagcggacgg atactggatt gtcgccggaa
     5161 gcctgcagag gcctcttcgg tgagctgcgg atcttggatt ggcttctgga cagtcacact
     5221 cttggtccaa gagtcgccgt agaatcgtgg ttggggccta cgggtgcacc tcaagatctc
     5281 gcagtcgggc gttgtgcaat tgaagtcaaa acgacggcag caaagcagga acagcggctt
     5341 aggattagca gcgagaagca gctggattgg gcacttgtcc cacatctgtt tctcgcccat
     5401 gtgtcagtcg tcgaggccat agatgagggg atcagcttgg acgaaatggt cgagaaggtt
     5461 cggcaacggc ttggtgacaa cccagggaga tccctctttg agacacggct cttggaagct
     5521 ggatactcgg acgaagacag ggagcgatat gaacaaccgc acttcgtggt ctcgaatacg
     5581 ttgatgtacg aagtccgcga agggttcccc tgtatcacgg cgaagtcggt gccggcagga
     5641 gttggcgacc tgacgtactc gattggcgtt ggatcgtgcc aggcgtttgt ggtagcaccc
     5701 ggggctgttc ttggatgcat tcggggggca gcggcatgct gacgctggat cagtatgccg
     5761 acaacttgcg gcaggaggta ctgctggaaa gcgatgag
//
LOCUS       DATMZD010000052         3925 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_41589,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000052 DATMZD010000000
VERSION     DATMZD010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41589"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="VN478_01060"
     CDS             <1..413
                     /locus_tag="VN478_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HWQ21299.1"
                     /translation="LTEDLDNRERRVAKRLRRQRDLLADMEEPIVLGNGPTLLVGWGS
                     SYGALEELVQNHPTDLRLVHFSEVWPLRTERLLAEMERARLVIAVEGNATAQLAQLIA
                     RETGRRISRSVLRTDGRPLSAAYIEGKLHDLEVI"
     gene            415..1263
                     /locus_tag="VN478_01065"
     CDS             415..1263
                     /locus_tag="VN478_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HWQ21300.1"
                     /translation="MWKNGMDIAWCPGCGNFALLEAVDRLLETSDIPRNHFLYVSGIG
                     QAAKLPHYLKGVNVLNGLHGRALPAAQAAKIANGDLTVVVHSGDGCNYGEGGNHLLAA
                     MRRNVDITLIVHDNQIYGLTKGQASPTTSLGTTTVAQTLGVMLEPFNPIAAAVALDCS
                     FVARTFVGDPEHLAATMHAAIRHHGFALVDVLQQCVTFNKVNTFGWYKERVQKLPEDY
                     DPTDRQAAFAKALEWGDHIPIGILYVHDRPTYGDLHPALGDKPALWRDWRTDLAPYDA
                     LLDEMT"
     gene            1274..2026
                     /locus_tag="VN478_01070"
     CDS             1274..2026
                     /locus_tag="VN478_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWQ21301.1"
                     /translation="MQTLRRILVTLIVITGLAAGGFVAWANTPAKPSAEAYAAMKSTD
                     DVKVSLDAGGRLLFEPIRAHNGVGFILYPGARVDPRAYAPLARGIAAGGSIVVIAPML
                     LNLAVLSPDRAQDVIDSYQGIRAWAVGGHSLGGSMAAHFARQHLKEVKGLVLWASYPA
                     STDDLSTTSLSVLSVTASRDGLATPAKIAAARPLLPPTTTYQPIDGGNHAQFGSYGTQ
                     MGDHSATITAPEQWHLIIQATSDFLAQVAKQQ"
     gene            2023..2997
                     /locus_tag="VN478_01075"
     CDS             2023..2997
                     /locus_tag="VN478_01075"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWQ21302.1"
                     /translation="MRRFARAFALGLAVLLLLLTFGPFLVPVHPYQGKTPYELADQDS
                     KFIYVRGLWLHYKTSGQGEPLILLLHGFGSSTFTWDRVAPALAKLGTVVYYDRPGFGL
                     SQRPLEQDWHGYDPYDSTEQAQFALELTHALGFRKAVLIGNSAGGGIALDMALKYPEA
                     VQAMVLVSPAVSSGNGANRFLKLLIHTPQVRHLGPLFVRALVPRMQSLITTAWHDPAR
                     MPSDVIPTYRKPLTASNWDAALWYVTSAPGQLDLWDDVGFIQPAALVVHGDDDRIIRQ
                     SDSRRLVSLNGRFTFVSLAGLGHVPQEESPQAFLDVVLPWLRTTVGLP"
     gene            complement(3049..>3925)
                     /locus_tag="VN478_01080"
     CDS             complement(3049..>3925)
                     /locus_tag="VN478_01080"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HWQ21303.1"
                     /translation="GSALTIGSRHAVWQGSSPTYRLGLLDLAWGVAPIKFGGQLYVPL
                     QELMEPMGAFVGKRASSDAVIVAYSTKWQREPWLRQGQLAQLETVDLARTNLKSVTTT
                     RKVVAFTFDDNWDAEAAVNIAKLFRAYNGHCTFFVVGTNVRMHPDAIRTMVRLGHDIG
                     NHTHSHPAATRIADATFIKQIQACEQVLNGMGLTTRPYFRFPYFAEDKHLMQIVTDQG
                     YLTVGSMWTIEDTGPKRSAALSVATVRRYISPGVIFVGHANSMATYDAMKAVLPELAR
                     QGYSFVSISELLRGS"
BASE COUNT          730 a         1309 c         1185 g          701 t
ORIGIN      
        1 atctcacgga agatctggac aaccgggaac gcagggtcgc caagcggctc cgcagacagc
       61 gggacctgct cgccgacatg gaagaaccga ttgtcctcgg caacggtccg accctgctcg
      121 tcgggtgggg atcgagctac ggggctctcg aagaattggt acagaaccac ccgaccgacc
      181 tccgtcttgt ccacttcagc gaggtctggc ccctgagaac tgagcggctc ctcgccgaga
      241 tggaacgcgc gcggctggtc atcgctgtcg aaggcaacgc caccgcccag ctggctcagc
      301 tcatcgcacg cgagacaggc cggcgcatca gccgctctgt cctcaggact gacggtcgtc
      361 ccttgagcgc tgcatacatc gaaggcaagc tgcacgacct ggaggtgatc tgacatgtgg
      421 aagaatggca tggacatcgc ctggtgtccg ggctgcggca acttcgccct gctggaagca
      481 gtcgatcgtc tccttgagac cagcgacatt ccacggaacc actttctgta cgtgtccggc
      541 attggccagg cagccaagct cccacactac ctgaaaggcg tcaacgtcct caacggactg
      601 catggacgcg ccttgccggc agcccaggca gcgaagatcg ccaacggcga cctgaccgtc
      661 gtcgttcact cgggggatgg atgcaactac ggcgaaggtg gcaaccacct gctcgcggcg
      721 atgagacgca atgtcgacat caccctgatc gtccatgaca atcagatcta cggtctcacc
      781 aagggccagg cgtcgccgac gacctcgctg ggcacaacga ccgtcgcaca gacgctgggt
      841 gtcatgctgg agccgttcaa cccgatagcc gccgcggtcg ctctggactg ctccttcgtt
      901 gcacggacgt tcgtggggga ccctgagcac ctggctgcca ccatgcacgc tgccatacgg
      961 caccacgggt tcgccctagt cgatgtgctg cagcaatgcg tcacgttcaa caaggtcaac
     1021 acattcggct ggtacaagga acgagtacag aagcttcccg aggattacga cccgaccgac
     1081 cggcaggcgg ccttcgccaa ggcgctcgaa tggggagacc atatcccgat cggcatcctg
     1141 tacgtgcacg accgacccac ctatggcgac ctccatcctg ctctgggcga caagcctgcg
     1201 ctgtggcgag attggagaac cgacttggcg ccatatgatg cactgttgga cgagatgacg
     1261 taggaagact cacatgcaga cgctcagacg catactcgtt acactcatcg tcatcacagg
     1321 cctcgccgca ggtggattcg tcgcgtgggc gaacacgccc gcgaagccgt cggccgaggc
     1381 ctacgctgcc atgaagagca ccgacgacgt caaggtctcg ctcgacgcgg gcggacgtct
     1441 gctgttcgag cccatcaggg cgcacaatgg ggtgggcttc atcctgtacc cgggcgcccg
     1501 cgtcgacccg cgtgcgtacg ctccgcttgc acgcggcata gcggcaggcg gaagcatcgt
     1561 cgtcatcgcg cccatgctcc tcaatcttgc cgtcctctcg ccggaccggg cgcaggacgt
     1621 catcgacagc taccagggga ttcgcgcatg ggccgtcggc ggccattcgc tcggtggatc
     1681 catggcggcg cactttgccc gccagcacct caaggaggtc aaggggctcg tcctgtgggc
     1741 gtcgtatccg gcgagcaccg acgacctgtc cacgacgtcc ctctccgtcc tgtctgtcac
     1801 cgcgtcccgg gacggactgg ccactcccgc caagatcgcg gcggcgcggc ctctgctgcc
     1861 cccgacgacc acataccagc ccatcgacgg cggcaaccat gcgcagtttg gctcgtatgg
     1921 gacgcagatg ggcgaccact cggccacgat caccgcccca gagcagtggc acctgatcat
     1981 ccaggcgacg agcgacttcc tggcacaggt ggcgaagcag cagtgagacg cttcgcacgc
     2041 gcatttgccc tcggcctcgc cgtcctgctc ctcctgctga cgtttggacc gtttctcgtc
     2101 ccggttcacc cctatcaggg caagacgccg tacgaactcg ccgaccagga cagcaagttc
     2161 atctacgtgc ggggtctgtg gctccactac aagaccagcg gacagggcga gcctctcatc
     2221 ctgctcctgc acggattcgg gtccagcacg ttcacctggg accgcgtcgc tccagccctg
     2281 gcgaaactgg ggactgtcgt ctactatgac cgcccgggtt ttgggctgtc tcagcgcccc
     2341 cttgagcaag actggcacgg ctacgacccg tacgactcga cggagcaggc gcaattcgcc
     2401 ctggaattga cgcatgccct gggatttcgg aaggcggtcc tcatcggcaa ctctgcggga
     2461 ggtggcatcg ccctggacat ggccctgaag tatcctgaag ccgtgcaggc gatggtcctt
     2521 gtcagtccgg ccgtgtcgag cggaaacgga gcaaaccgat tcctgaaatt gctcatccac
     2581 acgccgcagg tgcggcacct gggccctctt ttcgtcaggg cgctggtacc ccgcatgcag
     2641 tcactcatta caaccgcctg gcacgaccct gcccgcatgc cgtcggatgt catcccgacc
     2701 tatcggaagc ccctgaccgc aagcaactgg gatgctgcct tgtggtatgt cacgtctgcc
     2761 cccggacagc tggacctgtg ggatgacgtc ggcttcatcc agccagccgc gctggtcgtg
     2821 cacggcgacg acgacaggat catccggcag tcggacagca gacgcctcgt ctcgctgaac
     2881 ggccgattca ccttcgtgtc cctcgcagga ctcggccatg tgccacagga agagagcccc
     2941 caggcattcc tggacgtcgt gctcccctgg ctcaggacca ccgtcgggct gccgtgacat
     3001 ggccgcccat gacggacgtg agacgcagct tgttgcagcg ctgacgggtc agctgccgcg
     3061 caggagctcg ctgatcgaga cgaagctgta gccctgacgc gccagctccg gcaggacagc
     3121 cttcatggcg tcgtacgtcg ccatgctgtt ggcgtgcccc acgaagatga cgcccgggct
     3181 gatgtatcgc cggactgtcg cgacgctcag cgccgcagac ctcttcggac ctgtgtcctc
     3241 gatcgtccac atcgaaccca cggtcaggta tccctgatct gtcacgatct gcatgagatg
     3301 cttgtcctca gcgaagtagg gaaaccggaa gtacggccgc gtggtcagtc ccatgccatt
     3361 gagaacctgt tcacatgcct ggatctgctt gatgaaggtc gcatctgcga tcctcgtcgc
     3421 cgcggggtgg ctgtgtgtgt ggttgccgat gtcgtgtcca agccgcacca tcgtgcgaat
     3481 ggcgtcagga tgcatgcgga cgttcgtgcc aacgacgaag aaggtgcagt ggccgttgta
     3541 tgcacggaag agtttggcga tattgactgc cgcctcggcg tcccagttgt cgtcgaacgt
     3601 gaacgcgacc accttgcggg tcgtggtaac gctcttcagg ttcgtacgag ccaggtcgac
     3661 ggtctccagc tgggccagct gaccctggcg aagccacggc tctctctgcc acttcgtcga
     3721 gtacgcgacg atcactgcat ccgacgacgc cctcttcccg acgaaggctc ccatcggttc
     3781 catcagttcc tgcaggggga catacaactg accgccaaac ttgatcggcg caacacccca
     3841 ggcgaggtcc agaaggccaa ggcgatacgt gggcgagctc ccctgccaga cggcatgccg
     3901 ggacccgatg gtcaaagccg acccg
//
LOCUS       DATMZD010000053         3155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_11548,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000053 DATMZD010000000
VERSION     DATMZD010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11548"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(206..964)
                     /locus_tag="VN478_01085"
     CDS             complement(206..964)
                     /locus_tag="VN478_01085"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HWQ21304.1"
                     /translation="MKKRFAFAALQSIAIVAIAAVIAFSPALAARQSVKADTTTTVPT
                     PSVSVNGTGKITVKPNIAHISFGVFTHDETASAAQTANDAIMAKITAALKAAGIKDED
                     IQTNGYSLQPRWVWDKTNEKNVQSGYDMNHNVTVTVRTIANAGKIVSLIADNGANTVN
                     GISFDVDDATLEQTKLVALDLAMANARKRADVVAKAAGKTITSVQTVNVNENSYTPYP
                     MYTTKDMAASGSEAAPVEAGTLDVVMSVSVTYLF"
     gene            complement(1096..1923)
                     /gene="arsM"
                     /locus_tag="VN478_01090"
     CDS             complement(1096..1923)
                     /gene="arsM"
                     /locus_tag="VN478_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HWQ21305.1"
                     /translation="MSELPVDQKIKEIVKDKYGKIAAGSGCGCGSGCQPMPDDSVDDI
                     SKGLGYSDADLAAVPEGANLGLGCGNPLAFAALKEGDVVLDLGSGAGFDSFLAARKVG
                     TTGKVIGVDMTPEMIARATANAKSGGYDNVEFLLGDIEALPLPDDSVDMAISNCVINL
                     APDKEKVFRELYRVLRPGGRFMVSDLVLLKELPEKVRTDVEAYVGCVAGAVLKDQYLG
                     VISAAGFEDVRIVDQSCNAEVVDHQGNQLSPEDDRRADDLMRSVASIKVQGIKKTTN"
     gene            complement(1920..2210)
                     /locus_tag="VN478_01095"
     CDS             complement(1920..2210)
                     /locus_tag="VN478_01095"
                     /inference="COORDINATES: protein motif:HMM:NF040962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HgcAB-associated protein"
                     /protein_id="HWQ21306.1"
                     /translation="MADKKQKENCCTPQTAECCKVESVVTIDERGQMILPKDLREKAG
                     LVAGDKLVIATSMKDGKLAFLMMFRADDFNDMVKGAVRPAMRMICMDDEERK"
     gene            2461..2859
                     /locus_tag="VN478_01100"
     CDS             2461..2859
                     /locus_tag="VN478_01100"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HWQ21307.1"
                     /translation="MYRTRSVALVIAMILLVVLLAGCAAKATPTPGPSTGGNTITVTA
                     AGMAFDTSTITVSAGAHVTITFVNNDSGVPHNIAFYTSSAATTVIYKGARTTGVSTVT
                     YTFDAPTTPGTYFFRCDVHPSTMTGEFIVK"
     gene            complement(2881..>3155)
                     /locus_tag="VN478_01105"
     CDS             complement(2881..>3155)
                     /locus_tag="VN478_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21308.1"
                     /translation="TERTTGTTFCFDCARYPCRRLRQLDLRYRTRYGMSMLENLDRIR
                     EMGLERFMAVENTRWVCPSCGGLICVHDRRCYTCGAQYERPGVKTG"
BASE COUNT          569 a          978 c          921 g          687 t
ORIGIN      
        1 gtgtgtagtc gactctgtca ccccccccga gtcctttggc ccaaagcccc taggcatatt
       61 ggtccaatac gaagggtgtc tctgagggac gtactcggcg aaggtgggaa tccatgtcgc
      121 gcagactccg gcgtgtcgtc cgccgccaga tgcaaaaacc cccgcgcggg gcgggggttg
      181 tgttcagaac gtatgttcgc cgattctaga acagataggt gacgctgacg ctcatgacga
      241 cgtcgagggt cccagcttcg accggggctg cctcgctgcc tgatgctgcc atgtctttcg
      301 tggtatacat cgggtatggc gtgtaggagt tctcgttcac gttcaccgtc tggaccgagg
      361 tgatggtctt gccggctgcc ttggcgacga cgtccgctct cttgcgggca ttggccatgg
      421 ccaggtcgag agcgacaagc ttcgtctgct cgagcgttgc gtcgtcgacg tcgaacgaga
      481 tgccattgac ggtgttcgcg ccgttgtcgg cgatgaggct cacgatcttg ccggcgttgg
      541 cgatggtgcg gacggtgacg gtgacgttgt ggttcatgtc gtagccgctc tggacgttct
      601 tctcattggt cttgtcccac acccatcgtg gctgaaggct gtatccattc gtctggatgt
      661 cctcatcctt gatgcccgcg gccttgaggg cggcggtgat cttagccatg atggcgtcat
      721 ttgctgtctg cgcggcactt gccgtctcgt catgcgtgaa gacgccaaag ctgatgtgcg
      781 cgatgttggg tttgaccgta atcttgccgg tgccgttgac actgaccgac ggcgtgggaa
      841 cggtggtggt cgtgtcagcc ttcacggact gccgtgccgc aagagccggc gagaaggcga
      901 tgacggctgc aatggccacg atggcaatgg actgcagtgc tgcaaaagca aatctcttct
      961 tcatggcata cctcctgtaa tattcaagca aagtgtgcgc ctgccttcgt tcggccggtc
     1021 gcgtttcgtg ttcaccgcta ggacgactcc cgggcagggg aagttcccgg ctcccacgcc
     1081 agggattgtc gacaatcagt ttgttgtctt ctttataccc tgaaccttga tgctggcaac
     1141 cgagcgcatg aggtcgtcgg cacgacggtc gtcctcgggc gacagctgat tcccctggtg
     1201 gtccacgacc tctgcgttgc aggactggtc gacgatacgt acgtcctcga atccggccgc
     1261 actgatgaca ccgaggtact ggtccttcag gacagccccc gcgacacagc cgacgtacgc
     1321 ctcgacgtcc gtccgcacct tctcgggcag ttccttcaac agcacaaggt cggataccat
     1381 gaaccggcct cctgggcgaa ggacgcggta gagttccctg aataccttct ccttgtcagg
     1441 ggcaaggttg atcacgcagt tcgagatggc catgtcgacc gagtcgtcag gcagtggcaa
     1501 tgcctcgatg tcgcccagca ggaattccac attgtcgtac ccgccggatt ttgcgttggc
     1561 cgtcgccctg gcgatcatct ccggcgtcat gtcgacaccg atgaccttgc cggtcgtgcc
     1621 gaccttgcgg gcggcgagga agctgtcgaa cccggcgccg gatcccaggt cgagaacgac
     1681 gtccccttcc ttcagcgccg cgaatgcgag gggattgccg catccgagcc ccagatttgc
     1741 tccctcgggg accgccgcca ggtctgcgtc ggagtacccc agtcctttgc tgatgtcgtc
     1801 gaccgagtcg tcaggcatag gctgacaccc agaaccgcat ccgcatccag agccggcggc
     1861 tatcttccca tacttgtcct tgacgatctc cttgatcttc tggtccacag gcagttcgct
     1921 catttcctct cctcgtcgtc catacagatc atccgcatcg ccggccttac ggcccccttc
     1981 accatgtcat tgaagtcgtc cgccctgaac atcattagga acgccagctt gccatccttc
     2041 atggaagttg cgatcaccag cttgtcgcct gccacgaggc cggccttctc ccgcaggtcc
     2101 ttgggaagga tcatctgacc gcgctcgtcg atggtgacga cggattccac cttgcagcac
     2161 tcggctgtct gcggtgtgca gcagttctct ttctgcttct tgtctgccat gatacctcct
     2221 atccgtacaa tcagtacata ctgaaattgt ggatagtgct gaatccttgt caagacacct
     2281 gcagcacaat tagttcagga tcgacgttct ccttggaacc tggcttgcta cagaggcttc
     2341 acaatggcag cccaaccttc atggaaccat cgcacaggtg agtatactgc gtgcagattc
     2401 cccgaggaca cacccggcgg gtgtgccgtg tgaaggtttg caggtcagag gaggtactaa
     2461 atgtaccgca caagaagcgt tgcgctcgtc atcgcgatga tcctgcttgt cgtgctgctt
     2521 gcgggctgcg ctgccaaggc gacaccgacg cccggcccgt caacgggggg taacaccatc
     2581 accgtcaccg cggcggggat ggcattcgac acgagcacga tcaccgtttc cgcgggcgcg
     2641 cacgtcacga tcacctttgt caacaacgac agcggggtcc cacacaacat cgccttctac
     2701 acgtcatcgg ctgcgacgac ggtcatctac aagggggcaa ggacgaccgg cgtgtcgacc
     2761 gtcacctaca cgttcgacgc cccgaccacg cctggcacgt acttcttccg gtgcgacgtg
     2821 caccccagca cgatgacggg ggagttcatc gtcaagtaag aagcaggtta gtactggtaa
     2881 tcagcccgtc ttgacccctg gacgctcata ctgggcgcca cacgtgtagc agcgccggtc
     2941 gtggacgcag atgagaccac cacacgacgg gcacacccag cgggtgttct cgacggccat
     3001 gaaccgttcc agccccatct cacggatccg gtccaggttc tccagcatgc tcatgccgta
     3061 cctggtccga tagcgcaggt ccagctgtcg cagcctgcgg caggggtacc tggcacagtc
     3121 gaagcagaag gtagtcccgg tggtccgctc ggtac
//
LOCUS       DATMZD010000054         2590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_142278,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000054 DATMZD010000000
VERSION     DATMZD010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142278"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="VN478_01110"
     CDS             <1..643
                     /locus_tag="VN478_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWQ21309.1"
                     /translation="TGPTNVRGSDMFGRRRPDGPEFAPRPERPRVPPHQTVTTTFPTL
                     TAERVPSVDVNSLTLRFWGEVQGPAEMTWQQLMAVKHTTVAADFHCVTGFSMLGTEWG
                     GARVADVLEAADVHLSPSARFVMVHCVTEFTTNLPVQDLLADDTMLVWEWNRLPLTTA
                     HGGPLRLLVPSLYAWKDAKWVSGFEFLPEDRPGFWESRGYHMHGDPWKEERYG"
     gene            715..791
                     /locus_tag="VN478_01115"
     tRNA            715..791
                     /locus_tag="VN478_01115"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:749..751,aa:Ile,seq:gat)
     gene            816..890
                     /locus_tag="VN478_01120"
     tRNA            816..890
                     /locus_tag="VN478_01120"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:848..850,aa:Ala,seq:tgc)
     gene            911..2188
                     /gene="ablA"
                     /locus_tag="VN478_01125"
     CDS             911..2188
                     /gene="ablA"
                     /locus_tag="VN478_01125"
                     /EC_number="5.4.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009171835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050066 - lysine 2,3-aminomutase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046440 - L-lysine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HWQ21310.1"
                     /translation="MSGHKYTDVPLWKNVPQQDWDDWRWQVANRIQTVETLSRVVRLT
                     DEQKSGVISALSRFRMAITPYYATLIDQDNPDDPIFRQCVPTASELCSVPSDLDDPLY
                     EEVDSPVHGLTHRYPDRVLFLITDQCGMYCRHCTRRRLAGMTDGRRSRSEVDAAIGYI
                     RSHEEVRDVLLSGGDALLVDDEFLEYVLSEVRRIEHVEVIRIGTRAPVVLPQRITPEL
                     CAMLRKYHPLWLNTQFDHPNEVTPESSRAVAMLVDAGIPVGNQSVLLKGINDCPYIMK
                     ELVQKLVSIRVRPYYIYQCDLTSGISHFRTSVYKGIEIMEFLRGHTSGFSVPQFVVDA
                     PHGGGKIPVSPNYILSMSPDRIVLRNYEGVIVSYPEPEDTKSHCPKPCLICDTQKRKV
                     PPEGIAKIFTQDAYDLTPPGTLREKRREHYPKE"
     gene            2194..>2590
                     /locus_tag="VN478_01130"
     CDS             2194..>2590
                     /locus_tag="VN478_01130"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWQ21311.1"
                     /translation="MARKDYIDLKTAIPGPKGMEWYARDRAAISPSYVRPYVLVTDHA
                     EGMEVVDVDGNTYLDFTAGVAVNSTGHRHPEVVKAIKDQADRLVHMSGTDFFYPIQIV
                     LAEKIKEITPGPTEKMVWYGNSGAEAVEAG"
BASE COUNT          537 a          772 c          766 g          515 t
ORIGIN      
        1 gacagggcct acgaacgtgc ggggttcgga catgttcggc cgtaggcgcc cggacggacc
       61 cgagtttgca ccgcggcccg agcggccacg agtaccaccc catcagaccg tcacgaccac
      121 ctttccaact ctgacggcag agcgagtacc gtcggtggac gtgaacagcc tcacgctcag
      181 gttctggggt gaggtgcagg gtcccgccga gatgacgtgg cagcaactca tggcggtgaa
      241 gcacacgacc gtggcagcgg actttcactg tgtcacaggt ttttcgatgc tggggacgga
      301 gtggggcgga gcgcgggtag ccgacgtcct ggaagcagca gatgtccatc tgagtccctc
      361 agctcggttt gtcatggtgc actgcgtcac ggagttcacg acgaaccttc cggtccagga
      421 cctgcttgcc gatgacacca tgcttgtctg ggagtggaac cggcttcctc tcacgaccgc
      481 acatggcgga cccttgcggc ttcttgtgcc gtcactgtat gcgtggaagg atgccaagtg
      541 ggtatctggc tttgagttcc tcccggagga ccgaccgggg ttctgggaat cgcgtgggta
      601 tcatatgcat ggcgacccgt ggaaggaaga gcgctatggc tgagagccat tcctgcgact
      661 tgacagcggg gcgattccct gtagaatagc attttggaaa aggaagctgc aagtgggcct
      721 ctagctcagt tggttagagc gcgtccctga taaggacgag gtctctggtc cgaatccaga
      781 gaggcccacc atttgtattt cgtgtcttca cacgtgggga tgtagctcaa cgggggagca
      841 agtgctttgc aagcacgagg ttgcgggttc aaatcccgcc atctccacca tcgaaaggag
      901 gaggtgttcc ttgtcgggtc acaagtacac tgacgttccc ctttggaaga acgtaccaca
      961 acaggactgg gatgattggc gctggcaggt tgccaaccgc atccagacag tcgaaacgct
     1021 atcccgggtc gttcgtctca ctgacgaaca gaaatcgggc gtcatcagtg cgctctcccg
     1081 gttccgcatg gcgatcacgc catactatgc tacgctcatt gaccaggaca accctgacga
     1141 tcccatcttc agacaatgcg ttcctacagc gtcggagctt tgctctgtac ccagcgatct
     1201 ggacgatccc ctctatgaag aggtggactc cccggttcac ggcctgacgc atcgatatcc
     1261 cgatcgcgta ctcttcctca tcacagacca gtgtggcatg tactgtcgcc actgcacgcg
     1321 caggcggctt gcggggatga ccgacggacg gcgctccagg agcgaagtcg acgcggcaat
     1381 cggctacatc cgcagtcacg aggaagtgcg ggacgttctc ctgtccggcg gtgatgccct
     1441 gctcgtggac gacgagttcc tggagtacgt cctgagcgag gttcgcagga tcgagcatgt
     1501 cgaggtcatc cgcatcggca cgcgcgcccc cgttgtgctc cctcagcgca tcacgcctga
     1561 gctctgcgcc atgctgcgca agtatcatcc cttgtggctg aacacacaat tcgaccatcc
     1621 aaacgaagtg acgcccgagt cttcaagggc tgtcgccatg ctggtcgacg cggggatccc
     1681 ggtcggcaac cagtctgtcc ttctcaaggg gatcaacgac tgcccttaca tcatgaagga
     1741 actcgttcag aagcttgtct ccattcgggt ccggccgtac tacatctatc agtgcgattt
     1801 gaccagcggc atcagtcact tccggacctc tgtctacaag ggcatcgaga tcatggagtt
     1861 cctgcgcggc cacacgagtg gcttctcggt gccacagttc gtcgtcgatg caccgcacgg
     1921 cggcggcaag atccctgtca gccccaacta tatcctgtcc atgtctccag atcgtatcgt
     1981 tctccgcaac tacgaggggg tcatcgtctc ataccccgag cccgaagaca cgaagagcca
     2041 ctgtccgaaa ccgtgtctca tctgtgacac acagaagcgc aaggtgccgc ctgagggcat
     2101 cgcgaagatc ttcacgcagg acgcctacga cctgacaccg ccaggcacgt tgcgcgaaaa
     2161 gcgtcgtgaa cactatccaa aggagtgatg actatggcca ggaaggacta catcgacctc
     2221 aagaccgcaa tccccggacc caaggggatg gagtggtatg cgcgtgaccg cgctgctatc
     2281 tcgccatcat acgtccgccc atacgtcctg gtgaccgacc atgccgaggg catggaggtc
     2341 gtggacgttg acggaaacac gtatctggac ttcacggccg gagtcgccgt caacagtacc
     2401 ggccatcgtc atcccgaagt ggtcaaggcc atcaaggatc aggcagacag actcgtgcac
     2461 atgtctggca ccgacttctt ctatccaatc cagatcgtgc ttgccgagaa gatcaaggag
     2521 atcacgcctg gcccgacgga gaagatggtc tggtacggaa actccggggc cgaggctgtg
     2581 gaggctggac
//
LOCUS       DATMZD010000055         2963 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_41756,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000055 DATMZD010000000
VERSION     DATMZD010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41756"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /locus_tag="VN478_01135"
     CDS             complement(<1..855)
                     /locus_tag="VN478_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21312.1"
                     /translation="MRAKTRARAVTLRKSAGDVLDRLEAIGDAGQIRTESEVHLRTLL
                     TKTASALFKPEELPRYLQVQLAGKTGSIPSWDNLTGERLTHLGLPRHAEGVRADECGT
                     LVLTLVPATIDYAVQVEGREVCSSAAYDPRSTTTSFEDGIHRVVMTAPEVTVTRSAFP
                     HGLMDLPFVQVTFRLRNTSDQFHAVTLVLFIKPFDEIGVTSLERFSLSHENLIVLNGQ
                     RVAFARERPTRTKVTVYDPRSGSMVDADAGGEVVSPTGLMQLRLAFDHQLEPAGDTEV
                     TFFFFVDRD"
     gene            complement(845..2449)
                     /locus_tag="VN478_01140"
     CDS             complement(845..2449)
                     /locus_tag="VN478_01140"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008432969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HWQ21313.1"
                     /translation="MSKFVFVTGGVMSSLGKGIFSASLGRILIDEGFSVTILKMDPYL
                     NVDAGVQNPFEHGEVFVTEDGAETDLDLGHYERFLNQNLHRMNNITSGQVYLSVIEKE
                     RRGDYLGHTVQIIPHLTGEIRSRVMKVADTSKADFVIVEVGGTVGDIEGLPFLEAIRE
                     FWAEDPANSIFAHLTYVPYLETTDELKTKPTQHSVGELRKIGIQPTLVVARGVKELPV
                     DQKRKVALFCGVPADHVISLPNLSSVYKVPNYLQEQRVGHLVLSAFGMAPRIAETETP
                     WVRFTWTIDQAKTPRKLAIVGKYIQLNDAYLSLREALHHGAWATGGDLHIDLIDSEEV
                     EKQGTRLLDAGRYDGILVPGGFGKRGIEGMIMAAGYARTHGIPFMGICLGMQVASIEF
                     ARSVLGIADADSQEFRPDAKDLVIYLMPSQKGVQKLGGTMRLGVYENTITPGSRLEEA
                     YGSSTLSERHRHRYEFNNDYREAFEAHGMRVAAVYPEEDLVESLELEGHPFFVGVQFH
                     PELASKPLQPGPLFVAFVKAMLGHEG"
     gene            complement(2552..>2963)
                     /locus_tag="VN478_01145"
     CDS             complement(2552..>2963)
                     /locus_tag="VN478_01145"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWQ21314.1"
                     /translation="RRSSRTAGLRGWRPALSMYNEKGATMGFRENVEALLSRRHWSQR
                     ELAAAAGISPGRVSQILSNPTSPTLATATKIAAAFGLDVADMVRDERLRAPDDELETA
                     LRHQGDLSPQDIRAVHAFLAYIREARPKGNKRGR"
BASE COUNT          571 a          923 c          872 g          597 t
ORIGIN      
        1 gtcccggtcg acgaagaaga agaacgtgac ctcagtgtcg ccggctggct caagctggtg
       61 atcgaacgca agccgcaact gcatcaaccc ggttggggaa acgacttcgc caccagcgtc
      121 ggcgtcaacc atactgccgg accgcggatc atacaccgtg accttcgtcc gggtcggtcg
      181 ctcacgagca aaggccaccc gctggccgtt gagcacgatg agattctcat gggacaggct
      241 gaagcgttcc aggctagtga cgccgatctc gtcaaatggc ttgatgaaga ggacaagggt
      301 caccgcatga aactggtcgc tcgtattgcg cagccggaac gtgacttgaa cgaacggcag
      361 gtccatcagc ccatgaggaa acgcgctcct ggtcacggtc acttcgggcg cggtcatgac
      421 gacccggtgg atgccgtcct cgaaggaggt ggtcgtcgat ctcgggtcgt atgcagcaga
      481 ggaacagacc tctcgcccct ccacctgcac agcgtagtcg atggtggcag gcaccagtgt
      541 caataccagc gtgccacatt catcagcccg cactccctcg gcatgccggg gcaagcccag
      601 atgggtcagg cgctctccag tcagattgtc ccatgacggg atactccccg tcttgccggc
      661 aagctgtacc tgcaggtagc gcggcagctc ctccggcttg aagagcgccg atgccgtttt
      721 cgtcagcagc gtgcggagat gtacttcgga ctcagtgcga atctgtcccg cgtccccgat
      781 agcttccaga cgatccagca cgtcgcctgc agacttgcgg agagtgacgg cccgagctcg
      841 tgtcttagcc ctcatgcccc agcatggcct tcacaaacgc cacaaacagg ggacccggct
      901 gcagcggctt ggacgcaagc tcagggtgaa actggacgcc cacgaagaag gggtggcctt
      961 ccagttccag ggactcgacc aggtcctcct ccgggtagac ggcggcaaca cgcataccgt
     1021 gcgcctcgaa agcctcgcgg tagtcgttgt tgaattcgta gcgatgacga tggcgttcgg
     1081 acagcgtgga gctcccatat gcctcttcca gcctgcttcc cggtgtgatg gtgttctcgt
     1141 agacacccag ccgcatcgtt ccgcccagct tctgtacgcc cttctgtgac ggcatgaggt
     1201 agatgacgag gtccttcgcg tcaggcctga actcctggga gtccgcgtcc gcaataccca
     1261 gaacgctccg cgcaaactcg atactcgcca cctgcatgcc caggcagatg cccatgaacg
     1321 ggataccgtg agtgcgcgca tatcctgcag ccatgatcat cccttcgatg ccgcgttttc
     1381 cgaacccgcc cggcacgagg atcccgtcat acctgcccgc gtccaggaga cgcgtcccct
     1441 gcttctccac ctcttcgctg tcgatcaagt cgatgtgcag atcacctcct gtcgcccatg
     1501 caccatgatg cagggcttcg cggagagaca ggtatgcgtc gttcagctgg atgtacttgc
     1561 cgacgatggc cagcttcctc ggcgtcttcg cctggtcgat ggtccatgtg aaccgcaccc
     1621 agggcgtctc ggtctcggct atcctcggcg ccatgccgaa cgcggacagt accagatggc
     1681 ctacccgctg ttcctgcagg tagtttggga ccttgtacac agagctcagg ttgggcagcg
     1741 agatgacgtg atcggcgggg acaccgcaga acaaggcgac cttgcgcttc tggtcgacgg
     1801 gcagctcctt cactccccga gcgacgacca gtgtcggctg gatgccgatc ttacgcagtt
     1861 caccgacact gtgctgggta ggcttcgtct tgagttcatc ggtcgtttcg agatagggaa
     1921 cgtatgtcag gtgggcgaag atcgagttgg caggatcttc agcccagaac tcgcggatgg
     1981 cttccaggaa cggcagacct tcgatgtcgc cgaccgtgcc gccgacctca acgatcacga
     2041 agtccgcctt cgacgtgtcg gcgaccttca tgacacggct acggatctcc ccggtgagat
     2101 gcgggatgat ctgtaccgta tgccccaggt agtcgcccct ccgctccttc tcaatgacgg
     2161 aaaggtagac ctgcccgctg gtgatgttgt tcatgcggtg caggttctgg ttgaggaagc
     2221 gttcatagtg ccccaggtca aggtcggtct ctgcaccatc ttcggtgacg aagacttccc
     2281 cgtgttcaaa cgggttctgt acgccggcgt cgacgttgag gtacgggtcc atcttcagga
     2341 tcgtcacgga gaaaccctcg tcgataagaa tacgtcccag agatgcagag aagattccct
     2401 tgcccaacga tgacatgacg ccaccggtaa cgaagacgaa tttgctcatt ctgcctccac
     2461 aatcgactgg gatatgatac tgcgcaccat ggtgacgcgt gcgccgtgca tgagtggaga
     2521 cctcccggcc ggctcctgaa ccatggccac actatctgcc acgcttgttc ccttttgggc
     2581 gggcttcgcg aatatacgcc agaaaggcat ggactgcccg tatgtcctga gggctgagat
     2641 ctccctggtg acgcagagcc gtctcgagtt cgtcatcggg cgcacggagt ctctcgtcgc
     2701 gcaccatgtc tgcgacgtca aggccgaatg cagcggcgat cttggtcgcc gtggccagtg
     2761 taggagacgt aggattggag agtatctgtg agactctccc cggagagatg ccggcagcag
     2821 cagccagctc tcgctgactc cagtggcgcc ttgacagcag tgcctcgaca ttctctctga
     2881 accccatggt cgctcccttt tcattgtaca tggaaagggc cggccgccaa ccgcgcagcc
     2941 cggcagttcg tgatgaccgt ctt
//
LOCUS       DATMZD010000056         5891 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_41804,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000056 DATMZD010000000
VERSION     DATMZD010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5891
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41804"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            213..1118
                     /locus_tag="VN478_01150"
     CDS             213..1118
                     /locus_tag="VN478_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015907856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWQ21315.1"
                     /translation="MGQFLQQLINGIQIGSIYALIALGYTMVYGIVRLINFAHADIFM
                     VGAYVGFFLAPIMAKIFGGAFVPTIIAAMIVTGLLGFVMEKLAYRPLRYKSRLSALIT
                     ALGVSLFLENFCSMPFIFGPTFRKFPDLIVAKNIPIPGNLGLNISNVLLLDVGVSVVL
                     MVLLFWFVNKTLIGKQMRAVAFDKSTASLMGINIDVTISTAFIVGPALAGAAGILYGT
                     TYGMLASPFLGTWPGLKAFIAAVLGGIGNIPGAVLGAYIMGISEALATSVNSNLGYGI
                     AFAILIGILLIRPSGLLGKFMPEKV"
     gene            1120..2103
                     /locus_tag="VN478_01155"
     CDS             1120..2103
                     /locus_tag="VN478_01155"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWQ21316.1"
                     /translation="MSYLRAHRTVATVATFVLAYVVIKALNLTGILNAYYIQLIVLAG
                     INIVMTVSLGMVNGFTGQFSIGHAGFMAVGAYTSAMITTVWLHTSTANPWVGYPVFIV
                     AVLVGGLLAAGAGYLVGAPSLRLKGDYLAIVTLSANELIRTVIRVTDVLGGPRGLGGI
                     PKFTTLEVVFVFGITSVVLMRNYLFSSHGRSMKAVRDSEIAAEAMGINTTKQKVFVFV
                     FSAFFAGLSGGVFAHLLQFIHPDNFSFVKSLEYLIYLYVGGSTSISGAIVGPAIFTLL
                     PELMRGLQSWRLVIYPLILVLTMIFRTEGIMGLKEFGFILIPKRQRKEVSA"
     gene            2103..2867
                     /locus_tag="VN478_01160"
     CDS             2103..2867
                     /locus_tag="VN478_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWQ21317.1"
                     /translation="MALLDVQHLTQYFGGLRAVSDFNIQLECGEIVGLIGPNGAGKTT
                     VFNVLTGVYAPTKGTIVFDGQNIVGRKTHQITQAGIARTFQTIRLFDKSSVIDNIKIA
                     YHFRMTYGNTDSFLHTRKYWQGEQLVDDYAIDLLRLFHLENHADDISGSLPYGAQRRL
                     EIARALATKPKLLILDEPAAGMNPAEADELMNMIHWLRDKFDLTILLIEHQMRVVMGV
                     CERIKVMDFGETICEGVPEHIRNDPRVCEAYLGKGA"
     gene            2872..3576
                     /locus_tag="VN478_01165"
     CDS             2872..3576
                     /locus_tag="VN478_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013144323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWQ21318.1"
                     /translation="MLEVRNIDVYYGSIRALKGVSFTVEKGEIVTIIGANGAGKSTTL
                     RAISGILRAKSGSILYEGTDITHTPPHLIARMGVSHVPEGRGIFGNLTVQENLDVALS
                     SVKDKRTAGPNLDKVFSLFPRMKERRRQIAGTLSGGEQQMLAVGRGLMQNNPFILLDE
                     PSMGLSPVLVEEIFGIIVEINQLGKTVLLVEQNAFKALSIAHRGYVLETGSLVLSGTA
                     AELRDNPDVKKAYLGG"
     gene            complement(3672..4694)
                     /locus_tag="VN478_01170"
     CDS             complement(3672..4694)
                     /locus_tag="VN478_01170"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015230391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HWQ21319.1"
                     /translation="MQYERELRETARALVAPDKGILAADESVGTIGKRFAKLGIASTA
                     ETHRAYRDMLFTTPGIEEFVSGVILYDETIRQESLGGTLFPRLLAEKAVIPGIKVDTG
                     AVELALHPGEKVTQGLDGLRERLAEYRSLGARFTKWRAVITIGPGIPSKACVSANAQD
                     LARYAALAQEAGLVPIVEPEVLMDGDHDQERCEFVTSGVLRSVFSELARQGVMLEGML
                     LKPSMVVCGKSCAHQAGVQDVAEATVRTLRRAVPAAVPGIVFLSGGQSSELATAHLNA
                     MNKLGPHPWELSYSYGRALQEPALTIWHGEEANVAAAQAAFYHRSKCNSAARSGSYTP
                     ELEREQ"
     gene            complement(4756..5652)
                     /locus_tag="VN478_01175"
     CDS             complement(4756..5652)
                     /locus_tag="VN478_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21320.1"
                     /translation="MSDKSREPTNSAEDRKDRRQRERLAELTRSFKKDPRAFVQKVED
                     ACPTGTQPATALLDAAVVLAEQDEFKPALALLDRAAVLYESSHDNAGLAHVYVNMGPL
                     YGMQGEMEKGVSFSLKAEGLARSLPADPELTLHYASDLGGMYTEMEEWDKAMQYFQIA
                     CDTSKSMGNKELEADALLVMSQVAVAQGDAAVARKLAEKGGSLGRNLHDKLFQARALQ
                     TMGDASALDGDHGQAVVLFQQALDLEADQPDLELRQQLFWEMSESYEEAGDKELAEDY
                     RSRAADLDESDEDGDEPDDSAD"
     gene            complement(5683..>5891)
                     /locus_tag="VN478_01180"
     CDS             complement(5683..>5891)
                     /locus_tag="VN478_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21321.1"
                     /translation="LTPEQLAAIADETYRAKFAFKLANGFDFTKLTIPRRVFETPAVV
                     SGFDEAYMRRAIAFAAGQLASPRI"
BASE COUNT         1091 a         1935 c         1646 g         1219 t
ORIGIN      
        1 catcagggat gccatgaaga acacgaccta cgtaggcatc tccggcgcgt acaagttcga
       61 cgagaaccgc gaccccatca aggccgcagt catcatcaag atcgtgaacg gtcagcagtc
      121 gtacctgacg accgtcaacc cgtagcgcag cttgtatctc ggagcagcgg ggaccaggta
      181 ctccccgctg ctcccctgtc ggaggagaaa acatgggtca gttcctgcag caactcatca
      241 acggcataca gatcggcagt atctatgcct tgatcgctct aggctacacg atggtctacg
      301 gcatcgtgcg ccttatcaac ttcgctcacg ccgacatctt catggtcggc gcctacgtgg
      361 gcttctttct tgcccccatc atggccaaga tcttcggcgg agccttcgtc ccgacgatca
      421 tcgcagccat gatcgtcacg ggtctcctcg gcttcgtcat ggagaagctg gcatatcgcc
      481 cactgcgcta caagtcccgc ctctcggcgc tcatcacggc tcttggcgtg tcactgttcc
      541 tcgagaactt ctgctcgatg cctttcatct tcggcccgac attccgcaag ttccccgacc
      601 tcatcgtagc caagaacatc cctatccccg gcaacctcgg cctcaacatc tccaacgtgc
      661 tgctgctcga cgttggcgtt tcggttgtgc tgatggtcct gctgttctgg tttgtcaaca
      721 agacccttat cggcaagcag atgcgcgccg tggcattcga caagtcgacg gccagtctga
      781 tggggatcaa catcgacgtc accatcagca cggcgttcat cgtcgggcca gcccttgccg
      841 gcgcagcggg aatcctctac ggaacgacgt atggcatgct cgcctcaccg ttcctgggta
      901 cctggcccgg tctcaaagcc ttcatcgccg cggtcctggg gggcatcggc aatatccccg
      961 gagctgtgct cggcgcatat atcatgggca tctcggaggc acttgcgacg tccgtcaact
     1021 ccaacctggg gtatggcatc gcattcgcca tccttatcgg catcctgctg attcggccaa
     1081 gcggcctgct gggcaagttc atgccggaga aggtctgaca tgagctacct tcgagcacac
     1141 cgcactgtgg cgacggtcgc cacgtttgtg ctggcctacg tcgtcatcaa ggccctgaac
     1201 ctgacgggca tcctgaacgc ctactacatc cagctcatcg tcctcgctgg catcaacatc
     1261 gtcatgacgg tcagcctggg catggtaaac ggcttcaccg gccagttctc catcggacat
     1321 gccggattta tggccgtagg cgcctacacg tcagccatga tcaccaccgt ctggctgcat
     1381 accagcacgg ccaatccgtg ggtgggatat cccgtcttca tcgtcgcagt ccttgtcggc
     1441 gggctgctgg ctgccggggc aggctatctt gtcggcgcgc cttcgctgcg cctcaagggc
     1501 gactacctgg ccatcgtgac gctgtcggca aatgagctga tccgcacggt catccgcgtc
     1561 accgacgtcc tgggcggccc tcgcggactg ggaggtatcc ccaagttcac gacgctggaa
     1621 gtcgtctttg tgttcgggat cacgagcgtc gtcctcatgc gcaactacct cttctcgtcc
     1681 cacggacgtt ccatgaaagc ggtccgcgac tccgagatcg cagcggaggc catgggcatc
     1741 aatacgacga agcagaaggt gttcgtattc gtgttctcag ccttcttcgc cgggctttcc
     1801 ggcggcgtct tcgcgcacct cctgcagttc atccatccgg acaacttcag tttcgtgaag
     1861 tcgctggaat acctcatcta tctgtacgtc ggcggttcca cgtccatcag cggggccatc
     1921 gtcggcccgg cgatcttcac gctcctgcct gaactcatgc gcggcctgca gtcctggcgc
     1981 ctggtcatct acccgctgat cctcgtcctg accatgatct tccggactga gggcatcatg
     2041 ggcctcaagg aattcggttt catccttatt cccaagcgac agaggaagga ggtgagcgca
     2101 taatggctct cctcgacgtt caacatctca cgcagtattt cggcggcctg cgtgccgtgt
     2161 ccgacttcaa catccagctg gagtgcggcg agatcgtcgg actcatcggc cccaacggcg
     2221 ccggcaagac gacggtattc aacgtgctta ctggtgtcta tgcgcccacc aagggcacca
     2281 tcgtcttcga cgggcagaac atcgtgggca ggaagaccca tcagatcaca caggcaggga
     2341 ttgctcgcac gttccagacg atccgtctgt tcgacaagtc cagtgtcatc gacaatatca
     2401 agatcgccta tcactttcgg atgacctatg gcaacactga ctcattcctc cacacacgca
     2461 agtactggca gggcgagcag ctcgtggacg actatgccat cgacctcctg cgcttgttcc
     2521 acctggagaa tcacgccgac gacatctcgg gttcactccc ctatggggca cagcggaggc
     2581 tggagatcgc ccgcgctctt gccaccaaac ccaagctcct catcctggac gagcccgcgg
     2641 ccggaatgaa tccggcagag gctgacgaac tgatgaacat gatccactgg ctccgcgaca
     2701 agttcgacct cacgatcctc ctcatcgagc accagatgag agtcgtcatg ggcgtatgtg
     2761 aacgcatcaa ggtcatggac ttcggcgaga ccatctgcga aggcgtgcct gagcacatcc
     2821 gcaacgatcc gcgggtctgc gaagcatacc ttggaaaggg ggcctgacat gctgctcgaa
     2881 gtccgcaata tcgacgtcta ctacgggtcc atccgtgcgc tgaaaggcgt gtcattcacg
     2941 gtggagaagg gcgagatcgt gacgatcatc ggggcaaacg gcgccggcaa gtcgaccacg
     3001 ctccgcgcga tctcggggat cctccgggcc aagtccggca gcatcctgta cgaaggaacg
     3061 gacatcaccc atacaccccc gcacctcatc gcacgcatgg gcgtttctca cgtccccgag
     3121 ggacgtggta tctttggcaa cctgaccgtc caggagaacc tggatgtcgc tctgtcgtcg
     3181 gtcaaggaca agcgcacggc ggggccgaac ctcgacaagg tcttcagcct gttccctcgc
     3241 atgaaggaac gccggcgtca gatcgcaggg accctgtcag gaggcgaaca acaaatgctc
     3301 gccgtcgggc gcggcctcat gcagaacaac ccgttcatcc tcctggacga gccatcgatg
     3361 ggactgtcac cggtgctcgt ggaggagatc tttggcatca tcgtcgagat caatcagctt
     3421 ggcaagaccg tactgctcgt cgagcagaac gctttcaagg cactgtccat cgctcatcgt
     3481 ggctacgttc tcgagacagg gtcactggtg ctttccggga ctgccgccga actgcgcgac
     3541 aatccggacg tcaagaaggc ctacctgggc ggctagcctc cccacccctc ctccttctca
     3601 gcgttccggg cagcggggat accccgctgc ccggaacgtc gttttgatgg tcacctcgtg
     3661 atgacggcct gttactgttc acgctccagc tcaggcgtat atgaaccgct gcgtgccgcg
     3721 ctgttgcact tgctccgatg atagaaggct gcctgtgctg ccgcgacgtt tgcctcctca
     3781 ccatgccaga tggtcagcgc gggctcctga agcgctcttc catatgagta gctgagttcc
     3841 cacgggtgcg gtcccagctt gttcatcgcg ttcaggtgag cagtcgccag ctcggacgat
     3901 tgcccgcccg acaggaagac gatgcccggg acggccgcag ggacagcgcg acgcagtgtg
     3961 cgcacggtgg cttcggccac gtcctggacc cccgcctggt gcgcgcagga cttgccgcaa
     4021 acgaccatgc tgggcttgag gagcatgccc tccagcatga ccccctggcg cgcaagctcg
     4081 ctaaagacag acctcaggac acccgaagtc acaaactcgc accgttcctg gtcatgatca
     4141 ccgtccatga ggacctcggg ctcgacgatc ggtacaagcc cggcctcttg tgccagtgcc
     4201 gcgtagcgcg cgaggtcctg ggcattggcg ctgacacagg ccttgctggg gatgccggga
     4261 ccgatcgtga tgactgcacg ccacttggtg aaccgcgcgc ccagtgagcg gtactcggcc
     4321 agtcgttccc tcaatccgtc gagtccctgc gtcaccttct cgcctggatg aagtgccagc
     4381 tcgactgctc ccgtgtcgac cttgatgccc gggatgacag ccttctcagc cagcagacgg
     4441 ggaaacagcg ttccacccag ggactcctgg cgaatcgtct cgtcatacag gatgacgcct
     4501 gagacgaact cctcgatgcc gggcgtcgtg aacaacatgt cccggtaggc tcgatgcgtc
     4561 tcagcagtcg acgctatgcc aagcttcgcg aatctcttgc cgatggtccc gacactttcg
     4621 tcggccgcaa gaatgccctt gtccggggcc accagagccc tggccgtttc tctcagttca
     4681 cgttcgtact gcacgtcatc ctccttgccc gcaggccgtc aacgacaaca ctgcacatac
     4741 tcacctgaca cgtgatcagt cggctgaatc gtccggctcg tccccgtcct cgtcgctttc
     4801 gtcaaggtcc gcggcacggc tgcgatagtc ctccgccagt tccttgtctc ctgcttcctc
     4861 gtacgattcg ctcatttccc agaacagctg ctgacgaagc tcgaggtcgg gctgatcggc
     4921 ttcgaggtcc agagcctgct ggaacagcac gacggcctgc ccgtggtcgc cgtcgagggc
     4981 acttgcgtca cccatcgtct gtagcgcacg ggcctgaaag agcttgtcat gcagattcct
     5041 gccgaggctg ccgcccttct cggcgagttt gcgcgccaca gctgcatctc cctgagcgac
     5101 tgccacctgg ctcatgacga ggagggcgtc ggcctcgagt tccttgttgc ccatgctctt
     5161 tgaagtgtcg caggcgatct ggaagtactg catcgccttg tcccactcct ccatctcagt
     5221 gtacatgcca ccaaggtcgc tcgcatagtg cagggtgagc tcagggtcag ccggcagact
     5281 cctggcaagc ccttcagctt tcagtgagaa gctgacaccc ttctccatct cgccctgcat
     5341 gccgtacagg gggcccatgt tgacatagac atgagccagg ccggcattgt cgtggctgga
     5401 ctcgtacagc acggcagccc tgtccagcag cgccaacgcg ggcttgaact catcctgctc
     5461 cgcaaggacc accgccgcgt cgagcaaggc tgtcgcaggc tgcgtgcccg tcggacaggc
     5521 atcctctact ttctgaacga aggcccttgg atccttcttg aacgaacgcg tcaactctgc
     5581 gaggcgttcc cgctgtctgc ggtccttacg atcctctgca ctgttggtcg gttccctgct
     5641 cttgtctgac atgtgtggtc ctcctgtgct gctctgccgc tgctagatgc gcggactggc
     5701 cagctgaccg gcggcaaacg caatcgcgcg ccgcatgtac gcctcgtcga acccgctcac
     5761 gacggccggc gtttcgaaca cccgccgcgg gatagtcaac ttggtgaaat caaagccgtt
     5821 cgcaagcttg aacgcgaatt tggcacgata ggtctcgtct gcaattgccg ccagctgctc
     5881 cggcgtgaga t
//
LOCUS       DATMZD010000057         3912 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_142454,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000057 DATMZD010000000
VERSION     DATMZD010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3912
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142454"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /gene="fusA"
                     /locus_tag="VN478_01185"
     CDS             <1..801
                     /gene="fusA"
                     /locus_tag="VN478_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453670.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HWQ21322.1"
                     /translation="PAVIIDKNAETHETILSGLGEAHLQVIVERLQKKFGVNIDLEVP
                     RVPYRETIKGKAHVEGKHKKQTGGHGQFGHCWIDMEPNGRGGGWVFENKVFGGAIPKQ
                     FIPAIEKGMLETMSKGQLAGYPAVDIKVAVVDGSYHPVDSSEMAFKLAGSLAFKKGFE
                     QCNPILLEPIVALETIVDEKYMGDVMGDLTSKRGKISGMENVQGKQIIKASVPLAEML
                     NYGIDLGSITQGTGTYSMKVNHYEEVPERIAEKIIADAKATMVAEEEE"
     gene            818..>3912
                     /gene="rpoC"
                     /locus_tag="VN478_01190"
     CDS             818..>3912
                     /gene="rpoC"
                     /locus_tag="VN478_01190"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HWQ21323.1"
                     /translation="MRNNKIEALSLHLASPEEIRSWSHGEVTEAETLDYRSGKPKPHG
                     LFCEIIFGPTKSFQCRCGKLKGKSFEGQVCDKCHVEVADSNVRRERMGHIELHAPVAH
                     IWYFRNAVNYIALLLEMPSKAVEKVVYFGSWLVLDPGNAEGLLRRQLVTDEEFRERRI
                     KGEKFVAKKGAEAFLELLKDLDLYKMRDSLLLRIKGKSRQDRAKAVKQLDVVTALINN
                     SRKPTDMIMTRIPVIPPDLRPLVQLEGGRFASDDLNELYRRVINRNNRLGKMLDENAP
                     EILLQNEKRMLQDAVDALLDNSKRQYPVVNANGRPLRSLSDKLKGKEGRFRQNLLGKR
                     VDYSGRAVIVSGPQLKISQCGVPKEMALELFKPHVIYRLMREGVVGSMKVAKDIVEKP
                     TPEVWSILEKVVKGNVVLLNRAPTLHRLSIQAFEPVLIDEKAIQISPLVCTPFNADFD
                     GDQMAIHLPLSLAAQAEARLLMLSSYNIFSPASGNPMAGPVQDMVLGAYYLTYEIKEK
                     MKAMKWSSAPVYDEQDVLYLYESGKLRVHDPVLIRVNGVKIRTTAGRVVFNDELRATI
                     FVGEAKYRDFPFQNKTMPKKEIEAMILEFSRQFGVSKTCVLLDNIKALGFKYATDSGI
                     SIGLSDMEVPPERQHILDDADRTVRDINAFYDEGLLSYNDRYLKVVQVWQDKGAEIER
                     AVFRNLTEENSVRIMADSGARGSKKQIVQMTGIRGLMSDPSGKIIEFPIKSNLREGLS
                     VLEYFLSTHGTRKGQADTALKTSDSGYLTRRLVDVAHELVVREEDCTHSQAREIKIGN
                     LVVSSLSEELAGKIADEKVVNPLTGETIIQPGEEISWEQAKRLEALGLKFLKIQRYVG
                     GLTVQPVVDGGAVIVGLREQIAGRYAADDIVLRASTVKVNPVWSKTTLERILERPLAV
                     DVVSLDGERIVARKSDPLTEKVLAAAKRAGITSVEVVVDEDIVLVPKDGLIRDEAAIA
                     IEQYGITSVRLRSAITCQSENGVCAMCYGRDLSTGNDVQVGEAVGVIAAQSIGEPGTQ
                     LT"
BASE COUNT          880 a         1076 c         1166 g          790 t
ORIGIN      
        1 cctgctgtga ttatcgacaa gaacgcagag actcacgaga cgattctcag cggactgggt
       61 gaggcacatc tccaggtcat cgtggagcgt cttcagaaga agttcggcgt gaacatagat
      121 ctggaagtcc cccgtgtgcc ataccgcgag acgatcaagg gcaaggcgca cgtcgagggc
      181 aagcacaaga agcagacagg aggacatggt caattcggtc attgctggat tgacatggag
      241 ccaaatggtc gtggtggcgg atgggtcttc gagaacaagg tattcggagg agcaatcccg
      301 aagcagttca ttcccgccat tgagaagggc atgctggaaa ccatgagcaa gggccagctg
      361 gctggatacc ccgccgtgga catcaaggtc gctgtcgttg acgggtctta ccatcctgtg
      421 gattcgtcgg aaatggcgtt caagctggca gggtcgcttg cattcaagaa gggattcgag
      481 cagtgcaacc cgattctgct tgaaccgatc gttgcgcttg agactattgt cgatgagaag
      541 tacatgggcg acgtcatggg cgacctgacg agcaaacgcg gcaagatctc gggcatggag
      601 aatgtacagg gcaagcagat catcaaggcg tcagttccgc ttgcggaaat gctgaactat
      661 ggtatcgatc tggggtccat tacccaggga actggtacat actcgatgaa ggtcaaccac
      721 tacgaggaag tgcccgagcg tattgctgag aagatcattg ccgacgcgaa ggccaccatg
      781 gtcgccgagg aagaagagta gttcaaaggg aggaagattg agaaacaaca agatcgaggc
      841 tctatccctc cacctggctt caccggagga aatacgcagc tggtcacacg gagaggtgac
      901 cgaggctgaa acgctggact accggagcgg caaaccgaag cctcatgggc tattctgcga
      961 gatcatcttc ggtccgacca agagttttca gtgccgttgc ggcaagctca aggggaaaag
     1021 cttcgagggg caggtctgtg acaaatgtca tgtcgaagta gctgactcca acgtccgccg
     1081 tgagcgcatg gggcacatcg agctccacgc cccggtcgcg cacatctggt acttccgcaa
     1141 cgccgtgaac tacattgcgc tcctgcttga gatgcccagc aaggccgtcg agaaggttgt
     1201 ctacttcggc agctggctcg ttctggaccc gggcaatgct gaaggactcc tgcgaagaca
     1261 gctcgtgacc gatgaggagt tccgggagcg gaggatcaag ggcgagaaat ttgttgccaa
     1321 gaagggtgcc gaggcattcc tggagcttct gaaagacctt gacctttaca agatgcgcga
     1381 cagcctcctt cttcggatca agggcaagtc gcgtcaggac cgggcaaagg ctgtcaaaca
     1441 actcgacgtg gtaacggctc tcatcaacaa cagtcggaag cccacggata tgatcatgac
     1501 ccgtatcccg gtcattcctc cggaccttcg tcctctcgtc cagctcgagg gcgggcgctt
     1561 cgcctcggac gacctcaacg agctctaccg tcgtgtcatc aaccggaaca accgcctcgg
     1621 gaagatgctt gacgagaatg ctccagagat cctgctgcag aacgagaagc gcatgcttca
     1681 ggacgccgtg gatgccctgc tcgacaacag caagcgccag tatccggtcg tcaatgcgaa
     1741 tggccgtccc ctgcggtctc tcagtgacaa gctgaaggga aaggaagggc ggttccgtca
     1801 gaacctgctg ggcaagcgcg tcgactattc cggtcgtgcc gtcatcgtct ccgggccgca
     1861 gctgaagatc agccagtgtg gtgttcccaa ggagatggcg ctcgagctgt tcaagccgca
     1921 cgtcatctac cggctcatgc gcgaaggtgt cgtgggcagc atgaaggttg ccaaagacat
     1981 cgtcgagaag cctacgccgg aagtatggag tatcctcgag aaggtcgtca agggcaatgt
     2041 cgtcctgctc aaccgtgcgc caacccttca ccgcctctcg attcaggcgt ttgaacctgt
     2101 cctcatcgac gagaaggcta tccagatctc gcccctcgtc tgcactcctt tcaacgctga
     2161 cttcgacgga gaccagatgg ccatccatct tcctctgagt cttgcggccc aggccgaagc
     2221 ccgcctgctc atgctctcat cctacaatat cttctcgccg gcaagcggca accccatggc
     2281 gggccccgtc caggacatgg tacttggcgc ctactatctc acgtacgaga tcaaggagaa
     2341 gatgaaggcc atgaagtgga gttctgctcc cgtctacgat gagcaggacg tcctttacct
     2401 gtacgaatcc gggaagcttc gtgtccacga cccggttctg atacgtgtca atggcgtaaa
     2461 gatccgtacc acggcaggtc gggtcgtatt caacgacgaa ctccgggcca ccatcttcgt
     2521 aggcgaggcc aagtaccgtg acttcccatt ccagaacaag acgatgccca agaaggagat
     2581 cgaggcaatg atcctggaat tctcccgcca gtttggcgtg tccaagacct gtgtccttct
     2641 tgacaacatc aaggcgctcg gattcaagta cgcgaccgat tcgggaatat ccattggcct
     2701 gtctgacatg gaagtgccac ccgagcggca gcatatcctc gacgacgctg acaggaccgt
     2761 gcgcgacatc aatgcgttct acgatgaagg gcttctttcc tacaacgacc gttacctcaa
     2821 ggtagtccag gtctggcagg acaagggcgc ggagatcgaa cgagcggtgt tccgcaatct
     2881 gaccgaagag aactctgtcc gtatcatggc cgactccggc gctcgtggaa gcaagaagca
     2941 gatcgttcag atgacaggta tccgaggact gatgtcggat ccgtcgggca agatcatcga
     3001 gttccccatc aagtctaacc tgcgtgaggg gttgtcggta ttagagtact tcctgtcgac
     3061 gcacggtact cgcaagggac aggccgacac ggcactgaag acgtctgact cgggctacct
     3121 gacgcggcgt cttgtcgatg tcgctcacga actcgtggtg cgcgaagagg actgcacgca
     3181 ctctcaggcg cgtgagatca agatcggcaa tctggtggta tcctcgctgt ctgaggagtt
     3241 ggcaggcaag atcgccgacg agaaggttgt gaatcccctc acgggagaga caatcatcca
     3301 gcccggtgag gagatctcgt gggaacaggc gaaacgcctg gaggctcttg gcctcaagtt
     3361 cctcaagatc cagcgctatg ttggtggctt gactgtccag cctgttgtcg acggtggagc
     3421 cgtgatcgtc ggtctccgcg agcagattgc gggccggtac gcggccgatg acatcgtcct
     3481 tcgtgcgtct acggtcaagg tgaaccctgt ctggtcgaag acgacactcg aacgtatcct
     3541 ggaacgtccc cttgctgtgg atgtcgtatc tctcgatggc gagcgtatcg tggccaggaa
     3601 gagtgaccct ctcactgaga aggtgcttgc tgcagcgaaa cgtgctggta tcacttctgt
     3661 ggaggtggtt gttgacgagg acatcgtcct ggttcccaag gacggtctca tccgcgatga
     3721 ggcagccatt gccattgagc aatatggcat tacgagcgtt cgcctccgtt ccgcaatcac
     3781 ctgtcagagt gagaatggcg tctgtgccat gtgctacggc cgcgatctgt cgacgggcaa
     3841 tgatgtccag gtcggcgagg cggtcggcgt catcgctgcc cagtccattg gtgagcccgg
     3901 tacgcagctt ac
//
LOCUS       DATMZD010000058         2501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_21875,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000058 DATMZD010000000
VERSION     DATMZD010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21875"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="VN478_01195"
     CDS             <1..409
                     /locus_tag="VN478_01195"
                     /inference="COORDINATES: protein motif:HMM:NF012265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative dsRNA-binding protein"
                     /protein_id="HWQ21324.1"
                     /translation="GEDKLRDERKPAILADGFEALVAAIYFDSGMHAAEKFVGIVFDK
                     AIADLHNGAVLDHKTALQEETQRSVRLLPSYTTTVARGEDGSDVFTSLVHIGGVLYGE
                     GRGSSKKKAEEDAARAALEAFVAEHPRRGESGQ"
     gene            406..1377
                     /gene="ftsY"
                     /locus_tag="VN478_01200"
     CDS             406..1377
                     /gene="ftsY"
                     /locus_tag="VN478_01200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HWQ21325.1"
                     /translation="MSFLSRVKQLFGGDERVQDESLAETPETAVEPAQAVSPAATQSI
                     RSQTGRARRESADFDALEELLIDAGIDVDTAMSILQEVRKGGLLGRPTAEQVIDRIRG
                     KLTSVLDLDSTMHTIGSPAVVLVTGVNGVGKTSSIAKLANMFKRENRSVLLIAADTFR
                     AGAVDQLAILASRVGVPVMRSVEGQDPSSVVFNGLQKAVADKVDVVVIDTAGRLENKK
                     NLTSELQKVVRVIEVKFGLPLSETLLVIDATTGENALMQARAFSEAIPISGIVLTKVD
                     SGAKGGSIISIARELRIPIKLVCNGETLMSIKPFDKDEIVNMILLSE"
     gene            1435..1857
                     /locus_tag="VN478_01205"
     CDS             1435..1857
                     /locus_tag="VN478_01205"
                     /inference="COORDINATES: protein motif:HMM:NF016205.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21326.1"
                     /translation="MQSKPKEKVYITPGHEKKIKRKEEIAELLEFYSGLLTKKELGVL
                     ELYIQPSCSGAEVARKLRISRQAVHDHIRRSLGRMRRCESKLQLIANYKKNVVMFRKI
                     MLKLDECCEQSHNEEGERTLAELKTLFEKLINRNSHEL"
     gene            1867..>2501
                     /locus_tag="VN478_01210"
     CDS             1867..>2501
                     /locus_tag="VN478_01210"
                     /inference="COORDINATES: protein motif:HMM:NF012662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HWQ21327.1"
                     /translation="MFESVKKALGGVFQGLRRKGLLSQADIDAALVDVKLGLLGADVD
                     YRVVKSLIAQTREACLDEKVLKSITPGDQVVKVLYEQLTQALGGQGGKGIAITHIPFR
                     VMLVGLQGSGKTTTLGKLALLLKKEGHYPLLIPGDYGRPAAYQQLEKLAADAGVEFYG
                     DHADSLADLVRGADRFAREKSLDVQLLDTQGRHDFDTELMDELETVTRLYRP"
BASE COUNT          557 a          690 c          784 g          470 t
ORIGIN      
        1 gggtgaagac aagctgcggg atgaacggaa gccggcgata ctcgcagacg gtttcgaggc
       61 tctcgtcgct gccatctact tcgacagtgg gatgcacgcg gccgagaagt tcgtgggcat
      121 cgtgttcgac aaggcaatcg cagacctcca caacggggcc gttctggacc acaaaacggc
      181 attgcaggaa gaaacgcagc ggagcgtgcg cctgttgccg tcctacacca cgacggtcgc
      241 acggggcgag gacggatccg acgtgtttac gtcactggta cacatcggcg gtgtcctgta
      301 cggcgagggc cgcggatcgt ccaagaagaa agccgaggag gatgccgcgc gagcggccct
      361 tgaggcgttc gttgccgagc acccgcgccg aggcgagtcg ggtcaatgag tttcctgtcg
      421 cgagtcaagc agctgtttgg cggggacgag cgggttcagg acgaatcgct ggccgagacg
      481 cccgagactg cggtggaacc agcgcaggcc gtttctccag cggccacgca gagtatccga
      541 tcgcagaccg gccgtgctcg tcgcgagtca gcggatttcg atgccctcga agaactcctg
      601 atcgatgccg gtatcgatgt ggacacggcc atgtccatcc tgcaggaagt gcgaaagggc
      661 ggtctgctgg gccgtccgac ggctgagcag gtcatcgatc gtattcgcgg caagttgaca
      721 tccgtgcttg acctggacag cacgatgcac acgatcggtt cgcctgctgt cgttcttgtc
      781 acgggagtca acggtgtcgg caagacgtcg tcgatcgcca agctggcgaa tatgttcaag
      841 cgtgagaacc gctctgtttt gctgatcgct gctgatacgt tccgcgccgg ggcggtcgac
      901 cagctcgcca ttctcgccag tcgcgtcggc gttcctgtca tgcgctcggt ggaagggcag
      961 gacccgtcga gcgtcgtctt caacggactt cagaaagccg tagcggacaa ggtggacgtt
     1021 gtggtcatcg acactgccgg tcgcctcgag aacaagaaga accttacgtc cgaactgcag
     1081 aaggtcgtga gggtcatcga ggtgaagttc ggactgccac tgtcggagac actcctggtc
     1141 atcgacgcga cgaccgggga gaatgcgctg atgcaggccc gggcgttcag cgaggcgatc
     1201 ccgatctcgg gcatcgtgct gacgaaggtt gactctggcg caaagggcgg cagtatcatc
     1261 agtatcgccc gtgaactgcg catcccgatc aagcttgtct gcaatggtga aacacttatg
     1321 agcataaaac catttgacaa agatgagatt gtgaacatga tattgctgtc agaataagca
     1381 tcaatagcag cgaaaaccca tctattccgt cacatgccga taaggagctg aactatgcag
     1441 agcaagccga aggaaaaggt gtacatcaca ccgggtcacg agaagaagat caagcgcaag
     1501 gaggagatcg ctgagttgct tgagttctac tcgggactac tcaccaagaa ggaacttggc
     1561 gttctggaac tctacattca gccgagctgc tccggcgccg aagtcgcccg caagctgcgg
     1621 atctcaaggc aagctgtcca cgaccacatc cgtcgatcgc ttgggcgcat gagacgttgt
     1681 gagagcaaac tccagctgat cgccaactac aagaagaacg tggtcatgtt ccgcaagatc
     1741 atgctgaaac tcgacgagtg ctgcgaacag tcgcacaacg aagaaggtga gcggaccctt
     1801 gccgaattga agactctgtt cgagaagctg atcaaccgca acagccacga gctgtaggta
     1861 cacggcgtgt ttgagtcggt aaagaaggcg ctcggcgggg ttttccaggg gctgcgtcgg
     1921 aagggactgc tttctcaggc agacatcgac gccgcgctcg tagacgtcaa gctcggtctg
     1981 cttggtgccg atgtcgatta ccgggtcgtc aagtcgctta tcgcccagac acgtgaagca
     2041 tgcctcgacg agaaggttct caaaagcatt actcctggtg accaggtggt caaggtcctc
     2101 tatgagcagc tgacgcaggc gctcggagga caaggcggca aaggcatcgc gataacgcat
     2161 atcccattcc gggtgatgct cgtgggactc caggggtctg gcaagacgac gacgctgggt
     2221 aagctggcac ttctcctcaa gaaggaaggg cactaccctc ttcttatccc gggagactac
     2281 ggccgacctg cagcctacca gcaactggag aagcttgcgg ccgacgcagg ggtcgaattc
     2341 tacggtgacc atgccgacag ccttgcagac ctggtacgcg gcgctgaccg gttcgcccgt
     2401 gagaagtcgc tggatgttca gcttctggac acgcaggggc gccacgactt tgacaccgaa
     2461 ctcatggatg agctggagac cgtcaccagg ctgtatcgtc c
//
LOCUS       DATMZD010000059         5464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_133080,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000059 DATMZD010000000
VERSION     DATMZD010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5464
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133080"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VN478_01215"
     CDS             <1..190
                     /locus_tag="VN478_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21328.1"
                     /translation="SEIAGALGLRSSMNGSQKDYLTYSILGILMEQGRVIKDMSRMNG
                     VVYRVAPRVPGSAESSGA"
     gene            647..1612
                     /locus_tag="VN478_01220"
     CDS             647..1612
                     /locus_tag="VN478_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21329.1"
                     /translation="MDVQLDWLLEGPAWVRYRALVDLAHEPETSPQEQAARREMLADP
                     QVTGLVRELQGWPGVVLNSHKSAGQLYHKLRFAADLGLRRDDPGMPHVVHAAMAHQSE
                     QGPFQLSMAVSVAHGGTGHEDSGWALCDAPVIVGTLCELGLSSDPSVRRAVDFIAGLA
                     RDNGWPCAVSPELKDFRGPGRKGDPCPYATLACIEMELAAGIPHERQTRAGAEALLTQ
                     WSGRRESHPYMFYMGTDFSRLKAPFIWYDILHVADALSRIPWLRQDPRLQDMVRVMME
                     QVGTDGRATPGSVWMAWKDWEFGQKKVPSRWVTLVVERVRQRMSS"
     gene            1627..2262
                     /locus_tag="VN478_01225"
     CDS             1627..2262
                     /locus_tag="VN478_01225"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HWQ21330.1"
                     /translation="METIDLRKQMKDQWSPPVGKVVLVTVPAMPYLVIEGVGNPNTSK
                     VFQEAIQALYAAAYTMKFGAKTAGVADWKVMPMEALWWTTSGKDVSDADFEGKGGEDI
                     AWKALLMQPAVVTAEMLEQAKAEVVRKKKDVPALGRLHLETWAEGLCVQTMYVGPYDG
                     ERPTIEMMHAWIATNGYRARGRHHELYISDPGRTAPERMKTILRLPVEKIP"
     gene            complement(2404..4113)
                     /locus_tag="VN478_01230"
     CDS             complement(2404..4113)
                     /locus_tag="VN478_01230"
                     /inference="COORDINATES: protein motif:HMM:NF039835.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HWQ21331.1"
                     /translation="MRRLVALLIAVAATCSFTVPSLLLLPAPALGAASVWQKTSLDGG
                     QVHAVLVDPVNPGTVFAGTDRGLFRSTDRGSAWEQYGTGIKTGSSTTLKVFALAASPD
                     GGRLYAGTSAGAFWSADRGATWSQGSGMLSGSGIIRYYYVIAALAVDPAHPQTVYAGD
                     SGMMTEGRVLKSTDGGATWTTSASGISTHNVKALAVHPVRSSTVYAVVEDSLYISDDA
                     GAGWVKTTGRMEVNASEADVLTVMVTSDTVYAGTTAGVWRSRDGGSTWSQGTGLNSSG
                     ILKYFFGIAALAADPAKSSLMYAGDSGVMTSGGVYASTDAGASWTQAGGPLTEDISAV
                     AVDPSDPRTVYAATDGGLYRLTDVPSVSFQITASAPQGRGTVSPTAKEVAEGDTVTFS
                     LLADAGYHLTVLSDNGVSVTGLVRNNTYVLTGITADHVITAVFEPIPIILPPVSPHIL
                     VLTIGSSTMSVDGLRTTLDTPPIIVEGRTLLPIRAVIEALEGNVSWDPAARKATVALG
                     THTVEIWIGSRQARVDGGYVTLDVAAMIVNSRTLLPLRFVAENLGCLVTWDPVGRTVT
                     IEL"
     gene            4431..5186
                     /locus_tag="VN478_01235"
     CDS             4431..5186
                     /locus_tag="VN478_01235"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HWQ21332.1"
                     /translation="MRVIAAGWTDIGLQRRVNEDSFAVVQEGKEVELQGACYVVCDGL
                     GGAQAGEVASRTAVETFIAAWSSRELSAEDTKQRLRLAVHEANAVVYRLSLSSEELNG
                     MGTTLVALVPHAGRAYVCNVGDSRCYRLREGVLTQLTEDHTMAADMVRQGLLDPAYTR
                     EVSERNILTRAVGTAPRVEVDVVSDELSARDRYLLCSDGLWGTASESDLQAGLAGGSP
                     EEAAHRLVDLANTCGGPDNSTAVVVDVQDDRSG"
BASE COUNT         1030 a         1589 c         1856 g          989 t
ORIGIN      
        1 ctccgaaatc gctggcgctc tgggtctgag atcaagtatg aacggcagtc agaaggatta
       61 cctgacgtac tcgattcttg gaatactcat ggagcagggc agagtaatca aggatatgag
      121 tcgaatgaac ggtgtcgttt atcgcgtcgc tccgagagta ccgggttctg ccgagtcgtc
      181 gggggcctga cggccgaacc tgttcattgg agtagtggtt tgccccacta cccctgatcg
      241 tgattctcaa gggtctagac ccgatcagct gcggtctctg taaaggaggc cgcgaacatg
      301 gccgcgctga cttcctggct tcttgagggt tctgcgtggg tacggtaccg ggcgctgatg
      361 ggccgggcgc acgagtcgga ctagtccacg tggcatctgc accaaacgac atggaacaat
      421 tcttcctgct caatagatat ggtgttctct aggttgtggc agatgaacat tgttgcatcc
      481 gtcaggctgc gttgttgtac ataaatgtat gattcgtcga gtcgaaattg taccttttct
      541 ggcaaatgta tagttcctag tccgtctgcc cagttccttt gagtttgtag tccgcgaact
      601 acactgaaca cgaaggggca tctgctggga ttggcaggag gcattgatgg atgtgcagct
      661 tgattggctt cttgaaggtc ctgcgtgggt acggtaccgg gcgctggtgg acctggcgca
      721 cgagccggag acgtcgcctc aggagcaggc ggcgcggcgc gagatgctcg cggacccgca
      781 ggtgaccggc ctcgtgcggg agctgcaggg gtggccggga gtcgtcctca acagccacaa
      841 gtcggccggg cagctgtacc ataagctgag attcgctgcg gacctcggcc tgcgccggga
      901 cgatcccggg atgccgcacg tggtgcatgc tgccatggcg catcagtcgg agcagggacc
      961 gttccagttg tccatggccg tgtccgtggc tcacggtgga actgggcacg aggattcggg
     1021 atgggcgttg tgcgacgccc cggtcatcgt cggcacactc tgtgaactgg ggctgtccag
     1081 tgacccttca gtgcgccgtg ccgtcgactt catcgccggg ctggcgcgcg acaacgggtg
     1141 gccatgcgcc gtatcacccg agctgaagga ctttcggggt cctggacgca agggagaccc
     1201 ctgcccgtac gcgacgcttg catgcatcga gatggagctg gcagctggta tccctcacga
     1261 gcggcagacc cgggcagggg ccgaggcgct gctgacacag tggtcggggc ggcgcgagtc
     1321 tcacccctac atgttctaca tggggaccga cttcagcagg ctcaaggcac cgttcatctg
     1381 gtatgacatc cttcatgtgg cggatgcatt gtcccggatc ccatggcttc ggcaggaccc
     1441 gcgcctgcag gacatggtga gggtgatgat ggaacaggtt ggaaccgacg ggcgggcgac
     1501 gccgggatcg gtgtggatgg cgtggaagga ctgggaattt ggccagaaga aggtaccgtc
     1561 gcgctgggtc acgctcgtgg tcgagcgcgt gcgacagcgc atgagcagct gacggaggag
     1621 tcagacatgg agacgatcga tcttcgcaag cagatgaagg accagtggag tccgccggtg
     1681 ggcaaggtgg tcctggtcac cgtaccggcc atgccatacc tggtgatcga aggcgtgggc
     1741 aaccccaaca cgtcgaaggt cttccaggag gcaattcaag cattgtacgc ggcagcctac
     1801 accatgaagt tcggagcgaa gactgcgggc gtcgcagact ggaaggttat gccgatggag
     1861 gcgctttggt ggacgacgtc cggcaaggac gtgtctgatg cggacttcga agggaaaggc
     1921 ggagaggaca tcgcgtggaa ggcactgttg atgcagcctg cagtcgtaac ggcagagatg
     1981 ctggaacagg cgaaggcgga ggtcgtccgg aagaagaagg acgtgcccgc tctcggccgg
     2041 ctgcacctgg agacgtgggc tgaaggactg tgtgtgcaga cgatgtatgt tgggccgtac
     2101 gacggcgagc gtccaaccat cgaaatgatg cacgcgtgga tagccacaaa cggctaccgc
     2161 gcgcgcggac ggcaccacga gctctatatc agcgaccccg gccgcacagc gcccgagagg
     2221 atgaagacga tccttcgact gcccgtggag aagatcccct agcgggtcag ggagccgcca
     2281 tgtgcgccgc acgcagaagg ccgaagggaa gccagcgtgt cgtgcgcggc gtcacggtcc
     2341 ggttccggca cgtccgtggc gtgcaggcga tgtgctggaa gcgccgcagg cgaacctgac
     2401 cggctagagc tcgatcgtta ccgtcctccc gaccgggtcc cacgtgacga ggcagcccag
     2461 gttctcagcg acgaagcgga ggggcagcag cgtcctgctg ttgacgatca tcgcagcgac
     2521 gtccagcgtt acgtatcccc cgtcgacgcg tgcctggcgg gaaccgatcc agatctcgac
     2581 cgtatgggtg ccaagcgcca ctgttgcctt gcgtgctgcg gggtcccagc tgacattgcc
     2641 ttcgagggcc tcgatcacgg cacggatagg aagcagggtc cgaccctcca ctatgatggg
     2701 cggtgtgtcg agcgtggtgc gaagcccgtc gacggacatc gtcgacgaac cgatggtgag
     2761 taccagtatg tgcggcgaga ctgggggaag gatgatgggg atgggctcaa agacagccgt
     2821 gatgacgtga tccgcagtga tgccggtcag cacgtacgtg ttattgcgga ccagccccgt
     2881 tacgctgacg ccgttgtcgg acaggaccgt caggtggtat cccgcgtcgg cgagcaggct
     2941 gaacgtgacc gtgtcacctt ctgcgacctc cttcgccgtc ggcgagacgg tgcctcgccc
     3001 ttgtggcgca gaggcagtga tctggaagct gacagacggg acgtccgtca ggcgatacag
     3061 accgccatct gtggcagcat agacggtgcg cgggtccgaa gggtccacag cgaccgccga
     3121 aatgtcctca gtgagcgggc cgcccgcctg ggtccacgag gcgccggcgt cagttgacgc
     3181 atacacgccg ccagaggtca tgacgccact gtcgcctgcg tacatgagtg aggacttggc
     3241 cgggtccgca gcgagtgccg cgatgccgaa gaagtacttg aggatacccg aggaattgag
     3301 ccccgtcccc tgtgaccagg tgcttccgcc gtcccgggac ctccatacgc ccgccgtggt
     3361 ccctgcgtag acagtgtcag aggtcaccat gaccgtcagg acgtccgcct ctgaagcgtt
     3421 gacctccatc ctgccggtcg tcttcaccca gccggccccg gcgtcgtccg aaatgtacag
     3481 cgagtcctcg acgacggcgt agacggtcga cgacctgact ggatggacag ccagcgcctt
     3541 cacgttgtgg gtggagatcc ccgatgcgga tgtcgtccag gtcgctccgc cgtccgtgga
     3601 cttcaggaca cgaccctccg tcatcatccc gctgtcgccg gcatagacgg tttgcggatg
     3661 cgcagggtcg actgccagcg ctgcaatgac gtaatagtag cggatgatgc cgctgcccga
     3721 gagcatccct gaaccctgcg accacgtcgc tccccggtcc gccgaccaga aggctcccgc
     3781 cgacgtcccc gcgtacaagc gaccgccgtc cggcgacgcg gccagggcga agaccttcag
     3841 cgtcgtggac gagcccgtct tgatcccggt gccatactgt tcccaggcac ttccgcggtc
     3901 cgtcgacctg aacaggccac ggtcagtccc cgcaaagacg gttccgggat tcacgggatc
     3961 gaccaggacg gcatggacct gccctccgtc gagtgaggtc ttctgccaga cagatgccgc
     4021 tcccaaggct ggtgctggga gcaacagcag agaaggaacg gtgaacgagc acgtggcagc
     4081 cacggcaatc agcagtgcga ctagtcgtct catggatacc tcccgtacgt cactgacaga
     4141 caaggaagct ctctgtcccc attatagaca atcgattgga gggtcaatag cccggcacct
     4201 gggagcaaac gcttgcattc gatacggagt gcatatactt aattatgtcg gagtgcgaac
     4261 tctgacatgg cgaagaccag cgccccgcag agattgtggg gcgttcggct ttcctgggac
     4321 ggccccttga tcacggggcg cgaccgtgcc gctgcagcgg tctgactgca atctgtgctc
     4381 cgggcttgcg cccgtcgaca gggtcgaata tactcactca ggaggccgcc atgagagtca
     4441 tcgcagcggg ttggaccgat atcggactgc agcgtcgcgt caacgaggac agctttgccg
     4501 tcgtacagga gggcaaggaa gtcgagcttc agggtgcctg ctacgtcgtc tgcgacggtc
     4561 tcggcggagc tcaggcggga gaggtcgcct cccgcacggc cgtggagacg ttcatcgccg
     4621 cgtggtcctc gcgggagttg tcggccgagg acacaaaaca gcgtctgcga ctcgcggtcc
     4681 atgaggcaaa cgcggtcgtc tatcgtctgt cgctgtcgtc ggaggaactg aatgggatgg
     4741 ggacgacgct ggtggcgctt gtgccccacg ccgggcgcgc gtacgtctgc aacgtcggcg
     4801 acagcaggtg ctaccgtctc cgtgaaggcg tgctgactca gctgaccgag gaccatacga
     4861 tggcagcgga catggtgcgt cagggcctgc tggaccccgc gtacactcga gaggtcagcg
     4921 agcgcaacat cttgaccagg gcagtcggga ctgctccgcg cgtggaggtc gacgtggtgt
     4981 cggacgagct gtccgccagg gatcggtatc tcctctgctc ggacggactg tggggaacgg
     5041 cttccgagtc cgacctccag gccggtctgg ccgggggatc gccggaggag gccgctcatc
     5101 gcctggtgga cctcgccaac acatgcggag ggcctgacaa ctccacggcc gtcgtcgtgg
     5161 acgtccagga cgatcggtct gggtgagggc cgggtcctga gcatcgagca cacgccatga
     5221 acggcagccg gcgtcggtcg aatgccgtgt tcgcggcatg gtcatggttg tggccgcacg
     5281 ttctgctaca atagtggaca tgtcggcccg gccggaacga cggatgttcc attgcggccg
     5341 gtaattacca gctgacccca aaggagaggg aaatgagtga caggaagagc cagacgaccg
     5401 aagagcgcgt cgtcgccctg ctgaaggcga atcgcaagga cttgcgggcg ttcgtgcgcg
     5461 cggt
//
LOCUS       DATMZD010000060         3450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_122749,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000060 DATMZD010000000
VERSION     DATMZD010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3450
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122749"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1061)
                     /locus_tag="VN478_01240"
     CDS             complement(<1..1061)
                     /locus_tag="VN478_01240"
                     /inference="COORDINATES: protein motif:HMM:NF015568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HWQ21333.1"
                     /translation="MQSVFQFIRDFLGTPAVLVGMVALVGLVVLHKSFSEVLMGTVKT
                     IVGLLVLSAGAATLIGSLNYLTQMLQFGFHIQGILPNNEAMVVSAIKVLGAQPGWVMI
                     FGFLFNIFLARVTRWKYIFLSGHLMLYMGTVLVAVLTAAGMSGIWVTVVASVILGILM
                     TIMPALTQPFTRQVMGNDEIAVGQWGSLSYIAAGLLGKWFGDKTQSTEDMRLPKGLDF
                     LRDSLISAGVLMLILFVVVTLKAGPAFVRDTLGVTQHPLVFAFVQAMTFAAGMAIIIA
                     GVNMILVEITVAFRGIGEKIVPNAIPALDVPVVFPRAPMSMMIGFLSSLIAGFLCMLL
                     LQVLHLPVIIPGLVAHFFTG"
     gene            1262..2734
                     /locus_tag="VN478_01245"
     CDS             1262..2734
                     /locus_tag="VN478_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012900136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HWQ21334.1"
                     /translation="MTNETRHRSLLQDIAHRAMLERGLLPDFSPEVLAEVDKIQAPEP
                     TGDAHSQDLRAMQWCSIDNNDSLDLDQLTVAASLPGGQVKVMVATADVALLVSDGTAI
                     DGHARHNTTSVYTAAEKFPMLPDKLSTDLTSLNQDQDRMAVVVDMVLDKDGNLKTSDV
                     YRAIVRNHAKLTYSGTGAWLEGTGPVPDAVAAVPGLADNLRMQDAAAQDLKVLRQAMG
                     ALSLQTIQSRPVFEGDTIRGLEVELKNRATELIENFMIAANGITVRFLASRKSPCIRR
                     VVHTPKRWDRIVEVAAEQGVQLPTKPDAKALEAFLIEQKKAHPETFPDLSLTIIKLLG
                     PGEYVAQAPGEGAEGHFGLAVRDYTHSTAPNRRYPDLITQRLLKATLENAPVPYAFDE
                     LAALATHCTEQEDAANKTERQVAKSAAALLLQSRIGEQFNAIVTGAADKGTWARLLTV
                     PVEGRVVRGFEGLDVGQRTRVQLISVDVERGFIDLKRVGH"
     gene            complement(2869..>3450)
                     /locus_tag="VN478_01250"
     CDS             complement(2869..>3450)
                     /locus_tag="VN478_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008718769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HWQ21335.1"
                     /translation="ILLFRPLPGQLSEDAVRSMINPKKDVDCMSPVNMAKVFAGDESG
                     FAPCTSEAVMEVLAHYGIDVKGKRVTVVGRSMVVGKPLSMLLLKKNATVTICHTKTVD
                     LAGTCRGAEVVIAAVGKARMITDDFVSGNGVVVDVGINVDSDGRVCGDVDFDAVAPKS
                     SYITPVPGGVGAVTSSVLAKHVVRAANALNGMC"
BASE COUNT          761 a         1105 c         1004 g          580 t
ORIGIN      
        1 cccgtgaaga agtgtgccac gagccccggg atgatgaccg gcaggtgcag cacctggaga
       61 agaagcatgc agaggaagcc ggcgatcagg gacgacagga acccgatcat catggacatc
      121 ggagcacgcg ggaacacgac ggggacgtcc agcgccggta tggcgttggg cacgatcttc
      181 tccccgatcc cacggaacgc gacagtgatc tcgaccagga tcatgttgac gcccgcgatg
      241 atgatcgcca tgccggcagc gaacgtcatg gcctgcacaa acgcaaagac cagcgggtgc
      301 tgggtgacgc ccagcgtatc tctcacgaag gcaggacccg ccttgagggt caccacgaca
      361 aagaggatga gcatcagtac cccggcagag atcagtgagt cgcgcaggaa atccagccct
      421 ttgggcaatc gcatgtcctc ggtcgactgc gtcttgtcgc cgaaccactt gcccagcaga
      481 ccagctgcga tgtacgagag cgatccccac tggccaacgg cgatctcgtc gttccccatg
      541 acctgccgcg tgaacggttg cgtcagtgca ggcatgatcg tcatgaggat gcccaggatc
      601 acagaagcga cgacagtgac ccagatgccg gacatgccgg cggcggtcaa gaccgcaacc
      661 agcaccgtcc ccatgtagag catcagatga ccgctgagga agatatactt ccacctggtc
      721 acgcgcgcca ggaagatgtt gaacagaaac ccgaagatca tcacccatcc tggctgcgca
      781 cccaggactt tgatggcact gacaaccatt gcttcattgt tgggcaggat gccctgtata
      841 tgaaacccga actgcagcat ctgcgtcagg tagttcagtg acccgatgag cgtggcggcg
      901 ccagcactca gcaccagcag gccgacgatc gtcttgactg ttcccatcag cacttcgctg
      961 aatgacttgt gcaggacgac caggccgacg agcgcgacca tgcccaccag cactgcaggc
     1021 gttcccaaga agtccctgat aaactggaat actgattgca tgcccgtctc ctcttctcgt
     1081 cggcgcctgc agtctatcga cgccgaacag tacagcgcag ctcccattgt atagcaggtg
     1141 acgctcccga caatcgccac accctcagag aacgatcaat catgaatcga tccgtgtgac
     1201 gctgctatac tggggacaag actcaggcag agtctcactc tggaatggag gagtacctgc
     1261 aatgacaaac gagaccaggc accgctcact gctgcaagac atcgcccacc gcgccatgct
     1321 tgagcggggg ttgctccctg acttctcgcc tgaagtgctg gccgaagtcg acaagatcca
     1381 ggcaccggag ccgaccggcg acgcacactc acaagacctc cgggccatgc agtggtgttc
     1441 catcgacaac aatgattccc tggacctcga ccagctgact gtagcagcgt cactgcccgg
     1501 gggtcaggtc aaggtgatgg tcgccacggc cgacgtcgcc ctgctcgtga gcgacgggac
     1561 tgcgatcgac ggccacgccc gtcacaatac gacatcggtg tacacggcag cagagaaatt
     1621 cccgatgctg cccgacaagc tctcgaccga cctcacatcg ctgaaccagg accaggaccg
     1681 catggccgtc gtcgtcgaca tggtcctcga taaggacggg aacttgaaga cctctgacgt
     1741 ctaccgggca atcgtgcgca accatgccaa gctgacctat agcggcacag gggcatggct
     1801 ggaggggacc ggtcccgtgc cggacgcggt cgcagcggtt cccggtctgg ccgacaatct
     1861 gcggatgcag gacgcggctg cccaagatct caaggtgctg cgccaggcca tgggcgccct
     1921 cagcctccag acgatccagt cacggccggt cttcgaaggc gacacgatcc gcggactcga
     1981 agtcgagctg aagaacaggg cgacggaact catcgagaac ttcatgatcg ccgcaaacgg
     2041 gatcacggtc cgttttctcg cctccaggaa atccccgtgt atccgtcgcg tcgtgcacac
     2101 acccaagcgc tgggaccgca tcgtcgaggt ggccgcagag cagggtgtgc agctgccaac
     2161 taagcctgat gcaaaagcgc ttgaagcctt cctgatcgag cagaagaaag ctcatccaga
     2221 gacgttccct gacctctcac tgaccatcat caagctgctg gggccaggcg aatacgtcgc
     2281 ccaggcacca ggagaaggcg ccgaaggaca cttcggcctt gccgtgagag actacacaca
     2341 ttccaccgcg cccaaccggc gctatcccga cctcatcacc cagcgcctgt tgaaggcgac
     2401 cctggaaaac gctccggtgc catacgcctt tgacgaactg gcggcactcg caacacactg
     2461 caccgaacag gaggatgccg ccaacaagac ggagcgacag gtagccaaat ccgctgctgc
     2521 actgctgctc caatcccgga taggggaaca gttcaatgcg atcgtcaccg gcgccgccga
     2581 caagggaaca tgggcacgcc tgctcacggt tccggtcgaa ggcagggtgg tcaggggctt
     2641 cgaaggattg gatgtcggcc agcgcacacg ggtccagctc atctcggtgg acgtcgagag
     2701 gggattcatc gatctcaaga gggtcggcca ctagagccca gagtcacagg cgaagcgccc
     2761 tgtctcacaa tgaaccggcc cccgccgacg gaatgtcgct gggggccgga agtgtccttc
     2821 ctgggaaccc tgctgcacgc gcagcgacct tcgcgtatgc ccccccgatc agcacatccc
     2881 gttcagtgcg ttggcagctc gcacgacatg ctttgcgagc accgaggagg tgaccgcccc
     2941 cacgccgccg ggaaccggag tgatgtagga ggactttgga gcgacggcgt cgaaatccac
     3001 gtctccacac actctgccat cgctgtcgac gttgatgccg acatcgacca cgacaccgtt
     3061 cccggagaca aaatcgtcgg tgatcatcct ggctttgccc acagcggcaa tcacgacttc
     3121 ggcaccccga caggtccctg cgaggtccac ggtctttgtg tggcagatgg tgacggtcgc
     3181 gttcttcttg agcagcagca tggagagggg tttgccgacg accatgcttc tccccaccac
     3241 ggtgacccgc ttgcccttca cgtcaatccc atagtgggcg aggacctcca tgacagcctc
     3301 ggacgtgcac ggggcgaacc cgctttcatc tccggcaaag actttcgcca tgttgacggg
     3361 gctcatgcag tcgacatcct tctttgggtt gatcatgctc ctgaccgcgt cctcgctcag
     3421 ctgtccaggc agcggcctga acaggaggat
//
LOCUS       DATMZD010000061         3346 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_42081,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000061 DATMZD010000000
VERSION     DATMZD010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42081"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="VN478_01255"
     CDS             <1..465
                     /locus_tag="VN478_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21336.1"
                     /translation="RQHRWFDGKPADGMAAMAFHGEAEGQQSAESFYLHHGFAHVPGD
                     PDLMFKPFLPETVWTPTLLATRPTTALPDDQARVTVLLGPLSCPFQYRVLSQTGTYVA
                     EKLGIPVVEVDATGDSEGYVARGGRPGILVKGRLLEYDLWQREKLDQELAGL"
     gene            complement(547..1533)
                     /locus_tag="VN478_01260"
     CDS             complement(547..1533)
                     /locus_tag="VN478_01260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HWQ21337.1"
                     /translation="MDGRKQTLHMILALVAIAALLAGIVAVSHAVPAYSLVADDSLTP
                     AVDHPPPAQRVVPPIPLQPEPDLDKPLNILLLGADSREESDPGRTDTIMLVRLDPETK
                     HVRLLSLPRDTRVTIPGHGINKLNQASSGYYRDGGTSLLVETITTDLLPGLRIDYTVK
                     TDFAGFAAIIDALGGVTIDVEERMLYKGVDVSIDLQPGIQHLDGEQALEYARFRMDAI
                     GDFGTWSGEDHGRVARQKKLLAAVIDQTKDIRTLMRLPAIIRAVRAAVTTSMSFDVMA
                     RIAMTYKDVAYADVESVPFPGLPEYVDGISYVIPKTSVLQTTTGPLFGISPD"
     gene            complement(1936..2262)
                     /locus_tag="VN478_01265"
     CDS             complement(1936..2262)
                     /locus_tag="VN478_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HWQ21338.1"
                     /translation="MTKQPAVYILASRANGTLYTGVTSNLVKRVYEHRNNLADGFTKQ
                     YGVHRLVYYELHPDMIAAIGREKQIKKWNRQWKVNLIEQGNPEWCDLWPDITGDGQTG
                     FPHTRE"
     gene            complement(2509..>3346)
                     /locus_tag="VN478_01270"
     CDS             complement(2509..>3346)
                     /locus_tag="VN478_01270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HWQ21339.1"
                     /translation="PMVRLDKRINVLVLGCDARTKDDPGRSDSIMLVGLDPVTKKITM
                     MSIPRDTRVSIAGHGIGKINATTNKDVYADGGIPLLEKTIGGLVPGISVDYYVRTNFT
                     GFAEVIDALGGVTLDVEERMVYRAADTIIDLKPGVQHLDGDKALQYARFRADGVGDFG
                     SWGGEEHGRVARQKKLLKAVIAQTTSIRNAWRLPKVISVVQDAVVTDLSSSDMLRLGM
                     SFKDSTDKDVTTVVFPGIPKYVDGTSYVVPYTGPMKDKIAPLFKDAPVVSNPSAVTPN
                     SN"
BASE COUNT          650 a         1012 c          980 g          704 t
ORIGIN      
        1 cgccagcacc gctggttcga cggcaagcct gcggacggga tggctgccat ggccttccac
       61 ggcgaagctg agggtcagca gtctgccgaa tcgttctatc tgcaccacgg gtttgcacac
      121 gtccccggcg atccagacct gatgttcaag ccgttcctgc cggaaactgt ctggacgccg
      181 acccttttgg ccacgcgccc gacgactgcc ctgcccgacg atcaggctcg cgtcaccgta
      241 ttgctcggtc ctctttcgtg ccccttccag taccgcgtcc tgagccagac cgggacatat
      301 gttgccgaga aactcggcat cccggtcgtg gaagtggacg ccacgggcga ttctgagggc
      361 tacgtggctc gcggaggccg ccccggcatc cttgtgaagg gtcgcctgct ggaatatgat
      421 ctctggcagc gcgagaagct tgaccaggag ctggccgggt tgtaggcggc cgaacggtca
      481 ggtactcaat gaaagagccc cgtacctcgg ggctctttcg cgcatagtgg aggtgacaga
      541 accggctcag tctggagaga taccgaacag aggccccgtc gtcgtctgga gcacgctggt
      601 cttcggaatg acgtatgaga tgccgtccac atactcaggc aatcccggga aggggaccga
      661 ctctacgtcc gcatacgcga catccttgta ggtcattgcg atccgtgcca tcacgtcgaa
      721 cgacatgctg gtcgtcacgg ccgcccgtac ggcacggatg atggcaggca ggcgcatcag
      781 cgttcgaatg tccttggtct ggtcgatgac tgccgccagc agcttcttct ggcgcgccac
      841 gcggccgtgg tcctcaccgg accatgtccc gaagtcgcca attgcatcca tcctgaaacg
      901 tgcatactcc agcgcctgct caccatcgag gtgctggatc ccaggctgca ggtcgataga
      961 tacgtcgacc cctttgtaaa gcatcctctc ctcgacgtcg atggtcacgc ctccgagagc
     1021 gtcgatgatg gcggcaaacc ctgcgaaatc ggttttgact gtgtagtcga tgcggaggcc
     1081 aggcagaagg tcagtcgtga tcgtctcgac cagcaggctg gtcccgccat cccggtagta
     1141 tccgctggac gcctgattca gcttgttgat gccatgaccg gggatcgtga cacgagtgtc
     1201 gcgcgggaga gaaagaaggc gcacgtgctt cgtctccggg tccagccgca ccagcatgat
     1261 cgtgtcggtc cgcccgggat cactctcctc acgggagtcg gcccccagca gcaagatgtt
     1321 gaggggcttg tcgaggtcag gctcgggctg gaggggaatt ggtggtacga cccgctgcgc
     1381 gggaggcgga tgatcgacag ctggcgtgag ggagtcatca gcgacaagag agtaggcggg
     1441 cactgcatgt gaaacggcca cgatgccggc aagcaacgcc gctatcgcca ctagtgccag
     1501 aatcatgtgc agagtttgct tccgcccgtc cataccgctc ctttgtgtcc atcagcagga
     1561 gattactgct tagtatacaa cgtcatgcaa cattcccaat gagagcgcat gttggtcgtc
     1621 cctgcacacg cgatcccctt gctttgccac taccgcgaac acgagtcctt tggtttgtca
     1681 ttccagaaca tgtgggaatc catcataggt acgacgcagg ttcccgcctt cgcgggaacg
     1741 acgacaggag acagagccac tggactgatg ccgttcccgt cttcgcgaga agcacccctc
     1801 gtcttgcaca gaaggactcg gggagaacga cggaaagaag tcctcggttc gtcattccag
     1861 tctctgtggg aacgacgacg agagacagag ccgctagatt ggtgccgttt ccacatgcat
     1921 ggatcctcta ctctgtcatt cccgcgtatg cgggaatcca gtctgtccgt ctccagtgat
     1981 atcgggccaa aggtcgcacc actcgggatt gccctgttca atcaggttga ccttccactg
     2041 gcggttccac ttcttgatct gcttctcgcg gccaatcgcc gcgatcatgt cagggtggag
     2101 ctcatagtag accaggcgat gaacgccata ctgcttcgtg aagccgtctg cgaggttgtt
     2161 tcgatgttcg tagacacgtt tgacaagatt gctggtaaca cccgtgtaca acgttccatt
     2221 ggctctgctg gccaggatgt agacagcagg ctgttttgtc acgatagtcc tcctctgcgt
     2281 gtgagtccgc ctgctctgtc attcccacac atgcgggagt ccatcatagg tacgacgcag
     2341 gttcccgcct tcgcgggaac gacgacaaga gacagagcca ctggactgat gtcgttcccg
     2401 tcttcgcgag aagcaccact cgtcttgcac agaaagactc gggggaaacg acggaagaga
     2461 gtcctcggtt cgtcattcca ttctctatgg gaacggcagg cgagtcgatc agttgctgtt
     2521 cggggtcacc gcagacggat tcgagacaac cggcgcatcc ttgaacagcg gagcgatctt
     2581 gtctttcatc ggccccgtgt agggcacgac gtacgaggtg ccgtctacgt atttcgggat
     2641 gccggggaag acaaccgtcg tcacgtcctt gtcggtggaa tccttgaagg acatccccag
     2701 ccgcagcatg tcagaggatg acagatctgt cacgaccgcg tcctggacga cactgatgac
     2761 ctttggcagt cgccatgcgt tgcgaatgct ggttgtctgc gcgatgacag ctttgagcag
     2821 cttcttctgg cgtgcaacgc gtccgtgctc ctctccaccc cagctgccga aatcgcccac
     2881 gccgtccgca cggaaccgtg catactgaag tgccttgtcg ccatcgagat gctggacgcc
     2941 cggtttcagg tcgatgatcg tgtctgcagc gcggtacacc atgcgttctt cgacgtccag
     3001 agtcacacct ccgagagcgt cgatcacctc tgcaaacccc gtgaagttcg tgcgcacgta
     3061 gtagtcgaca gagataccag gcacaagtcc tccaatggtc ttctccagca ggggaatgcc
     3121 accgtcggca tagacgtcct tgttggtcgt ggcattgatc ttgccgatcc catgtccggc
     3181 gatactcacc cgtgtatcgc gcgggataga catcatggtg atcttcttcg tcaccggatc
     3241 cagccccacc agcatgatgc tgtcggaacg cccggggtcg tccttcgtgc gcgcatcgca
     3301 ccccagcacc aggacattga tgcgcttgtc cagcctgacc atcggc
//
LOCUS       DATMZD010000062         3445 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_32218,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000062 DATMZD010000000
VERSION     DATMZD010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3445
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32218"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="VN478_01275"
     CDS             complement(<1..411)
                     /locus_tag="VN478_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorohydrolase"
                     /protein_id="HWQ21340.1"
                     /translation="MAELLVRNARIVYPATGGFVIDRGWLLARDGVIAGLAAGDAPPS
                     IMQPVSEREEIDAAGMTLLPGLLNTHMHLYSMYSRGISTGRMSRTFLDVLQDLWWRLD
                     RSLLKDACWMSAAYSGMDSLRCGTTTIVDHHASPS"
     gene            complement(421..963)
                     /locus_tag="VN478_01280"
     CDS             complement(421..963)
                     /locus_tag="VN478_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006305281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HWQ21341.1"
                     /translation="MEHNVVIAGFGGQGVMLIGELLARAGMEEGKEVTWLPSYGPEQR
                     GGTANCAVVVADEPIASPLLTEPQAAIVFNRQSFDKFEPVIEKGGLLIVDSTLIDKKP
                     SRADLDVVYIPATDIASEMGSSKFTNMVLLGAYLGKVNPISMASVHGALSVFLTGKKA
                     ALIEVNKRALQAGVDFVNKK"
     gene            complement(963..1721)
                     /locus_tag="VN478_01285"
     CDS             complement(963..1721)
                     /locus_tag="VN478_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014452747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HWQ21342.1"
                     /translation="MAEQMKVIYERPSSWANKVTHYCPGCTHGVAHRLVAELVDEMGL
                     VDKTIGVWPVGCAVLGYDYFDFDSVEAAHGRAPAMATGIKVTNPDNFVFTYQGDGDLA
                     SIGMAEIVHAAARGWPITVIFINNGNYGMTGGQMAPTTLLGQKTTTSPLGRTSQTNGF
                     PIRVAEMLAQLDGPAYIARVSLDSPLNIRKAKSALRKAFNAQVRGIGFSLVECLSTCP
                     TNWKMTPVESMERVRKEVMPYFPLGEFKVVEGVL"
     gene            complement(1726..2793)
                     /gene="vorB"
                     /locus_tag="VN478_01290"
     CDS             complement(1726..2793)
                     /gene="vorB"
                     /locus_tag="VN478_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HWQ21343.1"
                     /translation="MSDKVLMKGAEALAEGAVQAGCRYYFGYPITPQNETPEYMSRRL
                     PEVGGRFVQAESEIASINMVYGAACAGVRVMTSSSSPGISLMQEGISYISGSNVPCVV
                     MNAMRGGPGLGNIAPAQADYFQAVKGGGNGDYKRIVLAPSSIQEGVDLFVDAFELAEK
                     YRMVVMVIVDGLIGQMMEPVEFKTKIDVSTLKIPDWATVGRHGRDHANIITSLTLDAA
                     GLEKWNLSLQAKYARVQESESRIEEYQMGDAEYAIIAYGTMARIAKTTVRVARAKGIK
                     LGLLRPITVWPFPVKQIQAYVGKLKGALTVELAYRQLFEDVQLTLCGQMPLGWYGRGG
                     GVVPGPQEILAAFEEQFGKGR"
     gene            complement(2790..3074)
                     /locus_tag="VN478_01295"
     CDS             complement(2790..3074)
                     /locus_tag="VN478_01295"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HWQ21344.1"
                     /translation="MSSIVFWIRSGRKQNSEATWRSTLERVSIDEQRCKSCGLCVSVC
                     PVKILVIGDRLNERGFRPAQCTDQDKCTSCSSCALVCPDLAIELHREVKA"
     gene            complement(3025..>3445)
                     /locus_tag="VN478_01300"
     CDS             complement(3025..>3445)
                     /locus_tag="VN478_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21345.1"
                     /translation="IDVGGDAAGIGAVAQFRETIVAGGYSLYVVVNTLRPGMDSPGGL
                     KRMLAAIRSGARLEIAGLVANTNLQSETTAEDVQRGYDAVSALGDSEALPVVAVVVSS
                     EYSSKVEHRLTPGATRLLSVIRVFNRILDTVGSQTEF"
BASE COUNT          729 a         1097 c          921 g          698 t
ORIGIN      
        1 gctgggagag gcatgatggt cgacaatcgt cgttgtccca cagcggagag aatccattcc
       61 gctgtacgcc gcgctcatcc agcaggcatc cttgagcaga gagcgatcga ggcgccacca
      121 gaggtcctgc agcacgtcga gaaacgtgcg ggacatgcgc ccggtgctga tgccacgcga
      181 atacatggag tacagatgca tatgcgtatt gagaagtccg gggagcaacg tcatgccagc
      241 agcgtcgatc tcctccctct ccgaaacagg ctgcattatg gatggcgggg catcgcccgc
      301 ggctagccca gcaatgacgc catcccgcgc caggagccat ccccgatcga tgacgaagcc
      361 gcctgtcgct gggtagacga tacgagcgtt cctgaccagc aattccgcca tcccgcactc
      421 ctacttcttg ttgacgaagt cgacgccggc ctgcaaggcc ctcttgttga cctcgatgag
      481 agcagccttc tttcccgtca ggaacacaga cagagccccg tggacagatg ccatgctgat
      541 agggttgacc ttcccaagat aagcgcccag cagaaccatg ttggtgaact tcgagctgcc
      601 catctccgaa gctatgtccg tcgctgggat gtacacgacg tcaaggtcgg cacgagaggg
      661 cttcttgtca atcagagtcg aatcgacaat gagcagccct cccttctcga tgacaggctc
      721 aaacttgtcg aaggactgcc gattgaagac gatcgccgcc tggggctcgg taagcagtgg
      781 agaagcgatc ggctcgtcgg cgaccaccac ggcgcagttg gccgtcccac cgcgctgctc
      841 cggaccatac gagggcagcc acgtgacctc cttgccttcc tccatgccgg cgcgggcgag
      901 aagttcgccg atcaacatca cgccttggcc accgaatccc gcaataacga cattgtgctc
      961 catcacagca ccccttccac gaccttgaac tcaccaagcg ggaagtacgg catgacttct
     1021 ttgcgcacac gttccatcga ttccacgggg gtcatcttcc agttggtggg acaggttgac
     1081 aagcactcga cgagcgagaa cccgatcccg cgcacctgtg cgttgaacgc cttgcgcaat
     1141 gcactctttg ccttgcggat gttcaagggc gagtcaagcg agacgcgcgc gatgtaggcg
     1201 ggtccgtcga gttgagcaag catctcggcc acacggatgg gaaaaccatt ggtctgtgac
     1261 gttcgtccaa gaggactcgt cgtcgtcttc tgtcccagga gagttgttgg tgccatctgg
     1321 ccgccagtca tgccatagtt gccgttgttg atgaagatca cggtaatcgg ccagcctcgt
     1381 gccgcggcgt gcacgatctc cgccatgccg atgctcgcaa gatcaccgtc tccctgatag
     1441 gtaaacacga agttgtctgg attcgtgacc ttgattcctg tggccatggc cggagcacgg
     1501 ccatgcgccg cctcgacgct gtcgaagtcg aagtagtcgt atccaagaac tgcgcagcca
     1561 accggccaga ctccgatagt cttgtctacg aggcccatct catccaccag ctcagcgaca
     1621 agccgatggg cgactccatg tgtgcaccct gggcagtagt gcgttacctt gttcgcccac
     1681 gaactcgggc gttcatagat gactttcatc tgttcagcca tggctctacc gtcctttccc
     1741 gaactgctct tcaaatgccg cgaggatctc ctgtgggccc ggcacgacgc cgccgccccg
     1801 cccataccac ccaagaggca tctgtccgca caatgtcaac tggacatcct caaacagctg
     1861 cctgtaggca agctcgactg tcagggcccc tttgagtttg ccgacgtatg cctggatctg
     1921 cttcacaggg aatggccaga cagtgattgg gcgaagcagt ccgagcttga tgcccttggc
     1981 gcgtgcaact cgcacagtcg tcttcgcaat acgcgccatc gttccatagg caatgatggc
     2041 gtactcggcg tcgcccatct ggtactcctc gatgcggctt tcgctctcct ggacccgtgc
     2101 gtacttggcc tgcaggctga ggttccactt ctccagcccg gcagcgtcca gagtgagact
     2161 agtgattatg ttcgcatggt cacgaccatg ccgtcccacc gtcgcccagt ccggaatctt
     2221 gagcgtcgag acgtcgatct tggtcttgaa ctcgacgggt tccatcatct gaccaatgag
     2281 accgtccaca atgaccatca ctaccatgcg gtacttctcg gccagttcga atgcgtccac
     2341 aaacaagtcc acaccctcct ggatcgatga aggggcgaga acgatgcgct tgtagtcccc
     2401 gttgccgccc cccttgaccg cctggaagta gtccgcctga gcgggagcaa tgttgccaag
     2461 gccaggtccg ccacgcatgg cattcatgac cacacaaggc acattggagc cgctgatata
     2521 cgaaatgcct tcctgcatca gggaaatgcc agggctggaa gacgacgtca tcacacgtac
     2581 accggcacac gcagctccgt agaccatgtt aatggatgct atctcacttt cggcctgcac
     2641 aaatctgcca cccacctcgg ggagacggcg actcatgtac tccggcgtct cattctgagg
     2701 cgtgatggga tatccgaagt agtaacggca tcccgcctgt acggcacctt cagccagggc
     2761 ttccgctcct ttcatgagaa ccttgtcgct catgccttca cctccctgtg cagctcaatg
     2821 gcaagatcag ggcagaccag agcgcacgaa ctgcaactcg tacacttgtc ctgatccgta
     2881 cactgcgcag gccggaaacc acgctcgttc aaccggtcac caatgaccag gatcttgaca
     2941 gggcaaacgc tgacgcatag accacagctc ttgcaccgct gttcgtcaat agagactctt
     3001 tccaaggttg acctccaagt tgcttcagaa ttctgtttgc gacccgaccg tatccagaat
     3061 acgattgaag acacgaatca ccgacagaag cctggtcgca cctggcgtga gtcgatgctc
     3121 taccttactg gaatactccg atgacacaac tacagcgacc actggtagcg cctcactgtc
     3181 gccgagcgcg ctgacagcgt cgtagccacg ctgcacatcc tctgcggtcg tctctgactg
     3241 cagattcgtg ttggcgacaa gtccagcgat ttcaagccgc gcgccagatc gaatggctgc
     3301 aagcatgcgc ttcaagccgc caggactgtc catccccggc cgcagcgtat tcaccaccac
     3361 atacagactg tagcctccgg ctacgatggt ctcccggaat tgagcgacag ctccgatccc
     3421 agcggcatct ccaccgacat cgatg
//
LOCUS       DATMZD010000063         2223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_21971,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000063 DATMZD010000000
VERSION     DATMZD010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21971"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1140
                     /gene="fabF"
                     /locus_tag="VN478_01305"
     CDS             <1..1140
                     /gene="fabF"
                     /locus_tag="VN478_01305"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HWQ21346.1"
                     /translation="GPITRFDASAISCRIAGEVKGFNPEDYFERRDVGRTDRVQQLAL
                     AAAQEAILDSHLDLQAEDRERIAVYVTSGIGGGASFEGEVLQVAVKGPSRISPFLIPK
                     LLVDSIPATIAERFKLFGGTSSVVSACASGTQMLGEALEAIRRGDADVIVAGASDAAV
                     TMTGIGAFANMRALSTRNDDPAHASRPFDKERDGFVMGEGAGILILERLDHALARGAR
                     IYGEILSQAVTSDAYHMTAPDPEAKQIVRAMRTAIDRAGLTPLDIDYVNAHGTSTPAN
                     DRTESSALQTLFGSRAPLVPVSSTKSMTGHLLGAAGAVETVACMLTFKNRLIYPTINY
                     RTLDLECPLDYVPNVARPSDVRYILKSSFGFGGHNVSIVLGRYSA"
     gene            complement(1218..1409)
                     /locus_tag="VN478_01310"
     CDS             complement(1218..1409)
                     /locus_tag="VN478_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HWQ21347.1"
                     /translation="MKVSINQETCIGCGACVALCDAAFRLNDELGKAEVIPGADCGAA
                     GCCKEAAESCPVAAITVEE"
     gene            complement(1479..>2223)
                     /locus_tag="VN478_01315"
     CDS             complement(1479..>2223)
                     /locus_tag="VN478_01315"
                     /inference="COORDINATES: protein motif:HMM:NF015113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HWQ21348.1"
                     /translation="LSTLGVRPGSVGPMQTSVPVLIDEMVGQGVAYIVGAGKDGFHIR
                     NAFFGRDFSATIAADVRTVRAGDLCPVCGHPLHTATGIEVGHTFKLGTKYSVPLHANF
                     VDEDGSEKPYFMGCYGIGIGRTLAAIIEQHHDEKGIIWPMSVAPFQVIIVPLNKGDDG
                     VWHAALELYEGLERAGVEVVLDDRGESAGVKFNDADLLGFPIQVVVGNSFLKTGSFEV
                     SLRSNKTDKKAVPAADAVAFVADLVRTAG"
BASE COUNT          453 a          648 c          642 g          480 t
ORIGIN      
        1 ggcccgatca cacggttcga tgcgagcgcc atcagctgtc gtatcgcagg cgaggtgaag
       61 gggttcaatc ccgaagacta cttcgagaga cgtgacgttg gacgtaccga ccgtgtgcag
      121 cagctggcgc ttgcagctgc gcaggaggca atactcgatt cgcacctgga tttgcaggcg
      181 gaagaccgag agcgaatcgc tgtctacgtt accagcggga taggcggcgg tgcgtccttt
      241 gagggcgaag tgctgcaggt tgcggtcaag gggccttcac gcatctcccc gtttctcatt
      301 ccaaaactgc tggtggactc cattccagcg accatcgctg agcggttcaa gttgtttgga
      361 gggaccagtt ccgtggtcag tgcctgcgca agcggtactc agatgctggg tgaagcactt
      421 gaggctattc gacggggtga tgcagacgtc attgtggcag gagcatctga tgctgccgta
      481 acgatgacag gcatcggggc atttgccaac atgcgcgcac tatccactcg gaacgatgat
      541 cctgctcatg catcgcggcc atttgacaaa gaacgcgatg ggttcgtcat gggggaaggt
      601 gccggtatcc tgattctgga acggctggac catgctctgg ccaggggtgc acggatttat
      661 ggcgagatcc tgtctcaggc agtaacgtca gatgcatatc acatgacagc ccccgatccc
      721 gaggcgaagc agatcgtgcg ggccatgcgt actgccatcg atcgtgcagg cctgacgcca
      781 ctggatattg actatgtcaa tgcccatgga acgtccactc ctgccaatga ccgaacggag
      841 tcatcagcct tgcagacgct cttcggtagt cgtgccccac tcgttcctgt gagttctacg
      901 aagtcgatga ccggtcacct gctcggcgct gctggggctg tagaaacggt ggcctgcatg
      961 ctaaccttca agaacaggct gatctacccc acgatcaact acagaacgtt ggatctcgag
     1021 tgtccgctgg actatgtccc gaacgtcgca cgtccatcgg acgtccggta catcctgaag
     1081 agttcattcg ggtttggtgg tcacaatgtg tccatcgttc tcggacgcta ctccgcatag
     1141 ttaaccaagt gctggcaggc agaagccccg tcgacgtttc gtcgacgggg cttctcgata
     1201 cactgtggta tagcaggcta ctcttcgacc gtgatcgcag ccacgggaca tgactccgct
     1261 gcttccttgc agcaaccggc agcgccgcaa tcggcacccg ggatgacctc agccttgcca
     1321 agctcatcgt tgagtctgaa cgccgcatca caaagggcga cgcatgcgcc gcaccctata
     1381 caagtctcct gattgatcga aaccttcatg gttcctcctt tggcataaat gctgtaattc
     1441 tattctaact gctaatagtt gggtgtcaat ttgacctgct accctgcagt tcgcaccagg
     1501 tcggcgacga acgcgaccgc gtccgcggcc ggcactgcct tcttgtctgt cttgttgctg
     1561 cggagcgata cctcgaagct gccggtcttg aggaaggagt tgccgacaac gacctggatc
     1621 ggaaacccga gaaggtccgc atcgttgaac ttgacgccgg cagactcacc tcggtcgtcg
     1681 agaacgactt caacaccagc tcgttcaagc ccttcataga gttccagcgc cgcgtgccac
     1741 acgccatcgt cgcccttgtt gaggggaacg atgatgacct ggaacggagc gacggacatc
     1801 ggccagataa tgcccttttc atcgtgatgt tgctcgatga tggcagccag cgtgcgcccg
     1861 atcccgatgc cgtagcaccc catgaagtag ggtttctcgc tgccgtcttc gtcgacgaaa
     1921 ttggcgtgaa ggggcaccga gtacttcgtt cccagtttga acgtatggcc gacctcgata
     1981 cccgtggccg tgtgcaatgg atggccacaa accgggcaca ggtctcccgc tctgactgtc
     2041 ctgacgtctg ccgcgatcgt tgcgctgaag tctcgcccaa agaatgcatt cctgatgtgg
     2101 aacccgtctt tgcccgcccc gacgatgtag gcgacacctt ggccgaccat ctcgtcaatg
     2161 agaacaggaa ccgacgtctg catcggtcca acggagcctg ggcggacgcc gagcgtactg
     2221 agt
//
LOCUS       DATMZD010000064         2631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_32267,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000064 DATMZD010000000
VERSION     DATMZD010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32267"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..1080
                     /locus_tag="VN478_01320"
     CDS             64..1080
                     /locus_tag="VN478_01320"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HWQ21349.1"
                     /translation="MRIGILTGGGDCPGLNPAIRGAVFRALDFGDECVGIVQGWQGLL
                     SCETEPLDLARVDEIIDRGGTILGTSRTNPFKEPALVQQTIANISALGLDAVIAIGGD
                     DTLGVATKLAALGVKVVGVPKTMDNDLSGTDFTFGFDTSVSVALDAARRLKDTALSHR
                     RIIVLEVMGRHAGWVSLYTGIGSGADYTLIPEVPVDWTAMVTQVMTAYKRKKYAFIVV
                     SEGVEVMEAACEKLDDFGHMLLQNRGVGPEVARILEEKTGISTRSATIGHIQRGGSPT
                     LFDRILGSRVGVKAVEMIHEGDFGKMAALRGTEVIAIPLTEAVGKLKTVPQEWVDLLT
                     TFSK"
     gene            1083..2042
                     /locus_tag="VN478_01325"
     CDS             1083..2042
                     /locus_tag="VN478_01325"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HWQ21350.1"
                     /translation="MKRIGLLTSGGDASGMNAAIRAVARTAASMGVEAVGFERGYDGL
                     VSNTWIELNARSVSGILELGGTMLKSARSELFRTEEGRKQAALVVQQNRLDGMVIIGG
                     NGSQTGGYLLGRLGVPVVGVASTIDNDLYGFDYTIGFDTAVNIAIDAIDRIRDAAESH
                     DRVFVVEVMGRDNGSIALEAALATGADIVLTPELPFSIPQLITRLHDDVMAQKKHHII
                     VMAEGAGHAEDLSHYINANLPVECRATVLGYVQRGGSPTRFDRILASTSGEAAVVALS
                     EGHSDVVTGTRNGHIVLQDTLEAVTRRNLLKPELVELLKRVSI"
     gene            2068..>2631
                     /locus_tag="VN478_01330"
     CDS             2068..>2631
                     /locus_tag="VN478_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21351.1"
                     /translation="MGRVFKSLTFQIIVTALLIVGVSMAILSNRLIVEFQDELVNKET
                     ETLEAIGSQAVERWNRMYRELSSSYKFDELSQSKQNELMRVFLSKSYYDYIGVFKGNF
                     PRVSIGYRISLFDQSLVYLADASRNPLPKLTVALPLKAGTKILGYLVVDRDMPQVLAP
                     LQRVRFEAFKTSSVSIALSGAATVVLLT"
BASE COUNT          546 a          806 c          791 g          488 t
ORIGIN      
        1 cctacaatga aagcgcattg tatctatcat cagtagtatg tacgaatgga ggtcgcgtaa
       61 cagatgagaa tcggaatcct gacgggcggc ggggattgcc ccggactcaa cccggcgata
      121 cgtggcgcgg tgttcagagc gctcgatttt ggggatgagt gtgttggcat tgttcagggc
      181 tggcaagggc tgctgtcctg tgaaacggaa cccctcgacc tggcccgcgt cgatgagatc
      241 atcgaccgcg gcggcaccat cctcgggaca tcccggacca accctttcaa ggaaccggca
      301 ctcgtccagc agaccattgc caacatctcc gcactaggcc tcgacgccgt catcgcaatc
      361 ggcggagacg acaccctggg tgtagccacg aagctcgcag ctctcggcgt aaaggtggtc
      421 ggcgtcccga agacaatgga caacgacctg tcgggaacgg acttcacatt tggattcgac
      481 acctcagtga gtgttgccct ggatgcggcg cgcaggctca aggacaccgc cctctcccat
      541 cgccgaatca tcgtcctgga ggtcatgggc aggcatgccg ggtgggtctc gctctatacc
      601 ggcatcggct caggagctga ctacaccctc atccctgagg taccggtcga ctggactgcc
      661 atggtgacac aggtcatgac ggcctacaag cgcaagaagt atgccttcat cgtcgtgagt
      721 gaaggggtcg aggtcatgga agcagcctgc gagaagctgg acgacttcgg ccacatgctg
      781 ctgcagaaca gaggcgtggg gccggaagtc gccaggatcc tcgaggagaa gacggggatc
      841 tcgactcgtt cggccacaat aggccacatt cagcggggag gcagccccac gctcttcgat
      901 cgcatcctgg gatcccgcgt cggcgtgaaa gctgtcgaga tgatccatga gggggacttc
      961 ggaaagatgg ccgcgctccg cggcacggaa gtgatcgcca tcccgctgac cgaagccgtc
     1021 ggcaagctca aaacggtccc ccaggagtgg gtcgacctcc tgaccacctt ctccaagtag
     1081 tgatgaagcg catcggactt ctgaccagcg gcggcgatgc cagcggtatg aacgctgcca
     1141 tccgcgcagt tgcacgcacg gctgcgtcga tgggcgtcga ggccgtcggg ttcgagcgcg
     1201 gctacgacgg actggtatcc aacacgtgga tagaactcaa cgcacgcagc gtctccggca
     1261 tcctcgagct cggaggaacg atgctcaaga gcgcacgcag cgaactgttc cgcacggaag
     1321 aaggtcgaaa gcaggctgca ctcgtcgtcc agcagaaccg gctggacggc atggtcatca
     1381 tcgggggcaa cgggtcacaa accggcggct acctgcttgg ccggcttggc gtccctgtcg
     1441 tcggcgtcgc ctcgactatc gacaacgacc tgtacggctt tgactacacg atcgggttcg
     1501 acactgccgt caacatcgcc atcgatgcca tcgaccgtat ccgtgacgcc gcggaatctc
     1561 atgaccgcgt gtttgtcgtg gaggtcatgg gcagagacaa tgggtccatc gcactcgagg
     1621 cggctctggc gacaggagcc gatatcgtct tgaccccgga actgcccttc agcatcccac
     1681 aactcatcac gcgcctgcac gacgacgtca tggcacagaa gaagcaccac atcatcgtca
     1741 tggcagaggg ggcgggccac gccgaagatc tctctcacta catcaacgcc aatttgcccg
     1801 tggagtgcag ggcgactgtc ctcggctacg tccagcgagg gggcagtccc acgcggttcg
     1861 accgcatcct ggccagcacg tcgggcgaag cggcggttgt cgccttgagc gaaggccatt
     1921 cggacgtcgt cacggggacg cgcaatggac acattgtcct gcaggatacg ctcgaggccg
     1981 tcacccggcg caaccttctc aaaccggagc tcgtggaact gctgaagcgc gtgtccatct
     2041 agttcagctt cgtcgcggga ggatgaattg ggtcgagtct tcaagtcact gacctttcag
     2101 atcatcgtga ctgccctgct catcgtgggc gtctcgatgg caatcctgag caaccgcctt
     2161 atcgtcgagt tccaggacga gcttgtgaac aaggagactg agactctcga ggctataggg
     2221 tcacaggctg tcgagcgctg gaaccgcatg taccgtgaac tgtccagcag ctacaagttc
     2281 gacgagctga gccagagcaa gcagaacgag ctgatgagag tcttcctctc caagtcctac
     2341 tatgactaca tcggcgtgtt taagggcaac ttcccccggg taagcatcgg atatcgcatc
     2401 agcctgttcg accagagcct cgtctacctc gctgacgctt cccgcaaccc actccccaaa
     2461 ctgacggtcg ctcttccgct gaaggcgggc acgaagatcc tgggctacct tgtcgtggac
     2521 agggacatgc ctcaggtcct cgcacccctg cagcgtgtcc gattcgaggc gttcaagact
     2581 tcaagcgtct ccatcgctct cagcggagcg gcaaccgtcg tccttttgac c
//
LOCUS       DATMZD010000065         9309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_12028,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000065 DATMZD010000000
VERSION     DATMZD010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 9309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9309
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12028"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..1040)
                     /locus_tag="VN478_01335"
     CDS             complement(54..1040)
                     /locus_tag="VN478_01335"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF039236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HWQ21352.1"
                     /translation="MIRNQWYVILESREVQRGRALAVRRMGKDLVLWRDTRGRVCCMS
                     DHCIHRGAALGGGKVRGDCVECPFHGLQYGGDGRVTVIPANGRDAPVPATFKGEAYVV
                     REAHGYVYLWWGEPRSAYPELPFIPDLDDTKLVYGTWRDPWHAFYSRAIENQLDVVHL
                     PFVHYNTIGAGNKTVVNGPVATMDGDVLRIWVHNEQDHGQKPLRQDEMPVPQGPSQLT
                     FVFPNLWQNNLGEKARITAAFVCVDEQHSILYLRFYRSFARLPGVKQLFDWFTGLANI
                     TIAHQDRRVVRTQRPFVSSLNGGEKLVQGDLPIVMYRRRREQLQNEAQAPRV"
     gene            complement(1161..1556)
                     /locus_tag="VN478_01340"
     CDS             complement(1161..1556)
                     /locus_tag="VN478_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019419919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HWQ21353.1"
                     /translation="MDSGKSSLDGIDAYIAQFPDEVQERLRTLRKIIRQAAPEAVERI
                     SYRMPTFVLHGNLVHFAAFKNHIGLYPAPSGVDAFIAELSAYRSGKGSIQFPMDRPLP
                     AELIGRIVRFRVAENVRRADAKPKTKGQK"
     gene            complement(1585..2964)
                     /locus_tag="VN478_01345"
     CDS             complement(1585..2964)
                     /locus_tag="VN478_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWQ21354.1"
                     /translation="MRDESGTEQNVVDVRSRRPQGMAAFTIVWFGQVVSLVGTAMTGF
                     ALTLWAYRTTGLATALSMVAFFGFAPMIIMSPIAGVLVDRWNRKWTMALSDLASAIGT
                     LTILILFASGHLQLWHLYVVGALTAVFQSFQWPAYSAAISMMVPKTQYQRASGMISMA
                     ESGSGIAAPILAGALIGVVGFERLWVIFAIDLVTFAIALSTLVAVTIPNPPRTVPEKE
                     ESFLRQSTFGFRYIWNHRPLFNLQMMFFSSNLLASLCVTILPTMILARTGNNSTVLAT
                     VQSASAVGGVIGGAALTAWGGPKRKVDGVLLGMVFASLFGTVILGLGRSLPVWAVGGF
                     MSMLFLPMLNGSNQAIWQSKVPPSLQGRVFSIRLIIAQVTAPIAMLASGPLADKVFGP
                     AMLTSGPLAPVFSWLTGTGTGAGISLMFVLFGLAGAVAGMAGYLIPSVRNVERLIPDH
                     DTPTVPESV"
     gene            3105..4094
                     /locus_tag="VN478_01350"
     CDS             3105..4094
                     /locus_tag="VN478_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21355.1"
                     /translation="MQERLFREYCQRRQFDAATTEAAVQAVKDFEGYVTVVGVTLETV
                     TAGHLADYLEQLSQAGTATEERLIALARYAYVTHRNELFVQLVHVAEAPDILPVLRAR
                     IRQIAGDEAADRVFQGIHLPAEGSPIDAYPPVVEEIVGRIKDTLPDETRKQVLAGNMH
                     QIPETSFADMKERFQRNPDIEAFLADRHTRLVQELEDHMNQGTLWYEQEITPAVLQYV
                     REHPEIQSGVRHGDVIHVAKIPFNPQAYLDEKDPTRKRYEACHCPLARSSISAGRQAV
                     SPLFCYCSAGYEKLPFEAIFGEPVQVEVLESALGGSDHCRFAITIPERFRKTQ"
     gene            complement(4150..4446)
                     /locus_tag="VN478_01355"
     CDS             complement(4150..4446)
                     /locus_tag="VN478_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HWQ21356.1"
                     /translation="MTDLEEYQKARDRVQEIKGFYAHVAMYVVANLSLTVLNLATLKK
                     NDGVIWFVWPLIGWGVVLGVHAISVFGIGRFLGKGWEERHIGEEMKRQHMPTDD"
     gene            complement(4443..4910)
                     /locus_tag="VN478_01360"
     CDS             complement(4443..4910)
                     /locus_tag="VN478_01360"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013457701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HWQ21357.1"
                     /translation="MTTVGDTLPAFELQDQHGGTVRDTNLRGHWIVLYCYPKDMTPGC
                     SQEAMGFQDQQSEFLRLGFRIVGVSADSVASHTRFAAKDGLVFTLLSDPQHVLLEALG
                     AWKTKKLAGHEFVGTERTTFLVDPEGKIVREWRKVRVAGHIALVLAAAHEVRA"
     gene            complement(4962..6077)
                     /locus_tag="VN478_01365"
     CDS             complement(4962..6077)
                     /locus_tag="VN478_01365"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR00045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HWQ21358.1"
                     /translation="MAKVLVSMNAFKGSISTRAATDAVATAFQSAGFQVEKVYLADGG
                     DGTIDVAATLGARVEFVSTFDPLMRPIEAPVAWLGQTAVIEMAKASGMVLISAEERNP
                     MLTTTWGTGVLILHALEHGADKIILGVGGSATVDGGLGMLAALGYSVTDEAGNPSWRG
                     GDGLSRLARVVANSSLKRSRLVVASDVTSPLLGPTGAAPVFGPQKGATPDMVTLLEKG
                     LERLADITLEARGVRLHDMPGAGAAGGVGGAAVAWLGGHLQPGAELFMDLEDFDTRAV
                     GCDLLVTGEGSIDAQTVYGKAPVRVAQRFRKASPQGLTVAIAGAVRDRAQVREAGIDV
                     FVTTLDRPMSEREAMENGPALLMQAASELAHLLSLKP"
     gene            6249..6947
                     /locus_tag="VN478_01370"
     CDS             6249..6947
                     /locus_tag="VN478_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWQ21359.1"
                     /translation="MLIELNKTGRRYHMGQREFWALKDVTLSIPEAQLVTILGPSGSG
                     KSTLLNIMGGIDRADTGTVRVADIDLSHASERDLTLFRRRTVGFVFQFFNLVPNLTVR
                     ENIEVTSDIAGKPLPIDDVLDAIGLLDLANRFPAELSGGEQQRVSLARAVVKNPVLLL
                     ADEPTGSLDFATSRDILRLLVRVNETYHTTIVLVTHNAAIADITDRVIRLRSGEVEED
                     RLVGRLVQAEEVTW"
     gene            6944..9175
                     /locus_tag="VN478_01375"
     CDS             6944..9175
                     /locus_tag="VN478_01375"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWQ21360.1"
                     /translation="MNTLSKRFPRIVRSNRWLVYGSVTLVILACMLYASLNALVVDID
                     ASMAQFERESQIESATFVPQRPFADLSVFESDHGFAIERRREVDAAMDDGLTLRVFER
                     TTHVDIPVITAGRDLQADGEMLLGQTAATALKLPLGTTMTLLGRSFTVVGFATVPDYM
                     YPIRTTDNADILLDPKAFGIAVVTPTAMNDLAPEALTTWHIRTVDGTTIAARQALVET
                     VGLTYWQDVTANPRYTLATAKIEGATSVSTSMPLVILLLASLLLAAAQGRMVRMQRPQ
                     LGMLKALGYSTWELVRQELALPTFIALSGSVLGVLSSILTMRPMLTFMASYFSIPPYV
                     TTVPWKAAVVATVAPILFVVPVVALVAVRLLGQSPVKLMQTTALSTRPGLLERSFHLV
                     RGSFQNRFAVRAAVRALTRLTLLMLGAMLAGYLLLFGGAMRDSMNTLVQGAFQDVRYN
                     YTYMLTAPQLGNPWGGEALTIAPFTAHDSQLTFQVMGLPTTSTLFLPRDSTGQPIPID
                     RVIITRALAQKLDLQTGDSWTMQQSATGQEVTLTVDAVAEMYTTAAVIMPQEQLNGLL
                     GLPLGSFNALLSTTSLQIPARNVLMTLSMAQSRAAFDSIMEPLWIGLGAVAIVAVFIA
                     LMALSVVTTLMVEEERVTISLLKVLGYRPEELNRMVLGSGIVAVGAGFLISIPLLLAS
                     VAKLLTRTTSSISFSLPIRLSPLSILVCAVIMLATYLVSLRAARRKVLGVPMAISLKA
                     ARE"
BASE COUNT         1794 a         3046 c         2742 g         1727 t
ORIGIN      
        1 gtatgaaagg atggccccgg gagaatctgt cggggccgtt tccatctctg tgttcagact
       61 cttggggcct gagcctcgtt ctgcagctgc tcgcggcgcc tgcgatacat cacgatcggc
      121 aggtctccct ggaccagctt ctccccgccg ttcagactgc tgacaaaggg cctctgagtt
      181 cggacgacgc gacggtcctg gtgcgcgatc gtgatgttgg caagcccggt gaaccagtca
      241 aacaactgct tgacgccggg caagcgagca aaactgcgat agaagcgcag atagagaatg
      301 ctatgctgct catcgacaca cacgaacgcg gcggtgatac gtgccttctc tcccaggttg
      361 ttctgccaca ggttcggaaa cacgaacgtc agctgcgaag gtccctgggg caccggcatc
      421 tcgtcctgcc tcaagggctt ctgaccatga tcctgctcgt tgtggaccca gatgcgaaga
      481 acgtcgccgt ccattgtcgc aacagggccg ttgacgaccg tcttgttgcc cgccccgatc
      541 gtgttgtagt gaacgaatgg caagtgtacg acgtcgagct ggttctctat ggccctggag
      601 tagaacgcgt gccacgggtc ccgccacgtg ccgtagacga gctttgtatc gtccagatcg
      661 gggatgaacg gcagctccgg gtaggctgac cgcggttctc cccaccacag gtacacatag
      721 ccatgtgcct cacggaccac atatgcctca cccttgaatg ttgccgggac gggcgcgtcc
      781 ctgccgtttg cggggatcac ggtcacgcgg ccgtcaccac cgtattgcag gccgtgaaac
      841 gggcactcca cgcagtctcc cctgaccttg ccgcccccga gtgccgctcc gcgatggatg
      901 cagtggtcac tcatgcagca tacccggccc ctcgtatcac gccacagcac gaggtccttg
      961 cccatgcggc gtacggcaag cgctctcccc cgctggacct cgcgtgactc gaggatgacg
     1021 taccactggt ttcggatcac cgtgtcacct cccggcttgc agcactccag agatggcctg
     1081 caacgattgt gcgcaatgag tgtctgcgct cctcacacca gggctctatg ggtggaaatg
     1141 tgcaggaagg tcgatgccga tcacttctgt ccctttgtct tcggtttcgc atcagcacgt
     1201 ctcacattct cagcgaccct gaacctgacg atcctgccta tcagctcagc aggcaacggc
     1261 ctgtccatcg gaaactggat cgagcccttg ccactccggt atgccgacaa ctcggcgatg
     1321 aacgcgtcga ctccacttgg agcggggtac aagccgatgt gattcttgaa agcagcgaaa
     1381 tgtaccaggt tcccatggag cacgaacgtg ggcatccggt agctgatcct ctctacggcc
     1441 tcgggtgctg cttgcctgat gatcttccgc agcgtcctga gcctctcctg gacttcgtcg
     1501 ggaaactgcg caatgtatgc gtcgatcccg tcgaggctgc tcttgccgct gtccatcgtg
     1561 tctcctttgg tcttcacgac gttctcacac cgattcgggc accgtcggcg tgtcatgatc
     1621 ggggatgagc cgctcgacgt ttctcacgct cgggatcagg tacccggcca tgccggccac
     1681 cgcaccggcc agcccgaaca ggacaaacat gagcgaaatg ccggcgcccg tgccggtacc
     1741 cgtgagccag ctaaacacgg gggcaagagg accagacgtc agcatcgcgg gcccgaacac
     1801 cttgtcagca aggggtcccg acgccagcat ggcaataggg gccgtgacct gcgcgatgat
     1861 cagccggatc gagaacacgc gcccctgcag cgagggtggc actttcgact gccagatagc
     1921 ctgattgctg ccgttcagca tggggaggaa gagcatgctc atgaatccgc cgaccgccca
     1981 gaccggcagc gaccggccca gtcccaggat cactgttccg aacagggatg caaagaccat
     2041 gccgagaagg acaccgtcga ccttgcgttt gggccctccc caggccgtca gcgctgcacc
     2101 cccgatgaca ccgccgacag cagaagcaga ttgcaccgtc gcgagcacgg tcgagttgtt
     2161 gccggtccgg gccaggatca tcgtcggcag gatcgtaaca cagaggctcg ccagcagatt
     2221 cgacgagaag aacatcatct ggaggttgaa cagcgggcga tgattccaga tgtaccgaaa
     2281 accgaacgtc gactgcctga gaaagctctc ttccttctcc ggcacagtcc tcggcggatt
     2341 cgggatggtg accgcaacca gcgtgctcaa cgctatcgcg aaggtcacaa ggtcgatggc
     2401 gaagataacc cacaggcgct cgaagcccac caccccgatc agggcacctg caaggatcgg
     2461 tgccgcaatg ccggagccgc tttcggccat ggagatcatt ccactggcac gctgatactg
     2521 cgtctttggc accatcatgc taatggcggc actgtatgca ggccactgaa aactctgaaa
     2581 cacggccgtg agagccccga cgacgtacag atgccacagc tgcagatgcc cgctggcgaa
     2641 gaggatgagg atcgtcagcg taccgatggc agacgcaagg tcggatagtg ccatggtcca
     2701 cttgcgattc catcgatcta ccaggacacc cgcgatcggg ctcatgatta tcatcggggc
     2761 gaagccaaag aatgcgacca tcgacagcgc ggttgcgaga cctgtcgtgc ggtacgccca
     2821 caaggtcagc gcaaagccgg tcatcgcagt cccgacaagg gacacgacct ggccaaacca
     2881 cacgatcgta aacgccgcca tgccctgcgg ccttcgcgaa cgaacgtcca ctacgttctg
     2941 ttcagtccct gactcgtcgc gcacgcatgc ctccactgcc cgtcccggca ctcggtaact
     3001 tggccgctta cgttccgatt atacttgtgg caggaagtga tgcaacgcaa cacactcagc
     3061 cggccgcggg gcaaaccacc tgcggcggcc aggaggcagg ggccatgcag gagagactct
     3121 tcagagagta ctgtcagagg cggcagttcg atgccgccac gaccgaggca gccgtgcagg
     3181 cggtcaagga cttcgagggg tatgtgacgg tggtcggcgt gacactggag acggtcacag
     3241 ccggccacct tgcagactac ctggaacagc tctcgcaggc aggcacggcc acagaagaac
     3301 gactgatcgc actggcgagg tatgcgtatg tcacgcaccg caacgagctc tttgtgcagc
     3361 tggtccacgt cgcggaggcg cccgacatcc tgccggtcct gcgagcacgc atcaggcaga
     3421 tcgcaggcga cgaagctgcg gacagagtct tccagggcat ccacctgccg gctgaaggct
     3481 cgcccatcga tgcctacccg cccgtcgtcg aggagatcgt gggcagaatc aaggacacac
     3541 tgcccgacga gacgcgcaag caggtactgg ccggcaacat gcaccagatt cccgaaacgt
     3601 ccttcgccga catgaaggaa cggtttcaga ggaacccgga catcgaggct ttcctggccg
     3661 accgtcacac ccggctggtg caggaactgg aagaccatat gaaccagggc acgctatggt
     3721 atgagcagga gatcacgcct gcggtgctgc agtatgtccg tgagcacccc gagatccaga
     3781 gcggcgtccg gcatggggac gtcatccatg tcgccaagat cccgttcaac ccccaggcct
     3841 acctggacga gaaggacccg acacggaagc ggtacgaggc atgccactgc cccctggcgc
     3901 gctcgtccat ctcagccggc agacaggccg tttccccatt gttctgttac tgcagcgccg
     3961 gctatgagaa gctccccttt gaggccatct tcggcgagcc cgtgcaggtt gaggtcctgg
     4021 agagcgctct tggaggcagc gaccactgcc gtttcgccat cacgatcccg gagcgattcc
     4081 gcaagacgca gtagtctgga acttcagcga gcaactgagg gacaccagaa acagctggag
     4141 cgcagcgggt cagtcgtccg tcggcatgtg ctgccgcttc atctcctcgc cgatgtggcg
     4201 ctcctcccag cccttgccca ggaaccggcc gatgccgaac accgagatgg catggacgcc
     4261 aagaacgaca ccccagccga tgagcggcca gacgaaccag atgacgccat cgttcttctt
     4321 gagtgtcgcg agattgagaa cagtcagcga taggttggcc accacgtaca tggccacgtg
     4381 agcgtagaat cccttgatct cctggacgcg gtccctcgcc ttctggtatt cctcgaggtc
     4441 cgtcatgccc gcacctcgtg cgctgcagcg aggaccaggg caatgtgtcc tgcaaccctc
     4501 accttgcgcc actcccgcac gatcttcccc tcggggtcga cgaggaacgt ggttcgctcg
     4561 gtccccacga attcgtgtcc ggcgagcttc tttgtcttcc acgccccgag agcttcgagc
     4621 agcacatgct ggggatccga aagcagggtg aagacgaggc cgtccttcgc tgcaaaccgc
     4681 gtatgggaag cgaccgagtc tgcactgaca ccaacaatgc ggaagccgag ccgcaggaac
     4741 tcactctgct ggtcctggaa acccatggct tcctgcgagc acccgggcgt catatccttc
     4801 gggtagcagt agagcacgat ccagtgtccc cgcagattgg tgtcccggac cgtcccgcca
     4861 tgctggtcct ggagttcgaa ggctggaagc gtatcgccga ctgtcgtcat gtctacctcc
     4921 tggtcgagag tgtcctcagt ataccccgga acggccatgc atcatggctt cagcgacaac
     4981 aggtgcgcca gctccgacgc agcctgcatc agcagtgccg gaccgttttc catcgcctcg
     5041 cgctcactca tggggcggtc gagggtcgtg acgaacacgt cgatgccggc ttcgcggacc
     5101 tgggcccgat cgcgcacggc cccggcaata gccacggtca acccctgtgg tgatgccttg
     5161 cggaagcgct gggccacacg gaccggcgcc ttgccgtaga ctgtctgtgc gtcgatactg
     5221 ccctccccgg tcaccagcag atcgcacccc acggctcgcg tgtcgaaatc ctcgaggtcc
     5281 atgaagagtt cggcgcccgg ctgaaggtga ccgcccagcc acgcgactgc agcgccgccg
     5341 acaccaccgg cggcgcccgc acccggcatg tcgtgcaggc gcacccctct tgcctcgaga
     5401 gtgatgtcag ccagccgttc cagccccttc tccagcagtg tcaccatgtc gggcgtcgcc
     5461 cccttctgtg gaccgaacac gggagccgcc ccggtcgggc cgaggagggg gctggtcacg
     5521 tcactggcga ccacgagcct gctccgcttc aatgacgagt tcgccacgac gcgggccagc
     5581 ctcgacaacc cgtcgccgcc ccgccatgag ggattcccgg cctcgtcggt gacgctgtag
     5641 cccagcgcag ccagcatgcc gagccctccg tccaccgtgg ctgagcctcc gactcccagg
     5701 atgatcttgt ccgccccgtg ttccagcgca tgcaggatca gtacacctgt gccccacgtc
     5761 gtcgtcagca tggggttccg ttcctcggca gagatcagca ccatcccgga cgcctttgcc
     5821 atctcgatga ctgcagtctg gccgagccac gccacgggtg cctcgatagg acgcatcagg
     5881 ggatcgaacg tgctcacgaa ctcgacgcgg gccccgagcg ttgctgccac atcgatagtc
     5941 ccgtcaccac cgtcggcaag gtagaccttc tcgacctgaa acccggcact ctggaacgcg
     6001 gtggccacag catcggtggc ggctcttgtg gagatgcttc ccttgaaagc gttcatactg
     6061 acaagtacct tcgccatggt atcccccttc gtgcctggtc ggcacacctt cattgtagcc
     6121 gcacccccat gcgatgccaa cggggcggca cttgacatgt caacacgtct gacgagaata
     6181 ctgaccggct ggtcactgac cacttggtcg gcggaccgca cgacagactg acaatgggga
     6241 gatgccacat gctgatcgaa ctgaacaaga cggggcgcag gtaccatatg ggccagcggg
     6301 aattctgggc tttgaaggac gtcacactca gtatccccga ggcgcagctc gtgactatcc
     6361 tcggcccttc cggttccggc aaatccacgc tattgaacat catggggggc atcgaccgcg
     6421 ccgacacagg cactgtccgt gtcgccgaca tcgacctgtc ccatgcgtcc gaacgtgacc
     6481 ttacgctgtt ccgacgtcgc accgtgggct tcgtgttcca gttcttcaac ctcgtcccca
     6541 acctgacagt ccgcgagaac atcgaggtga catcagacat cgcagggaaa cccctgccca
     6601 tcgacgacgt tctcgacgcc atcgggctgc tcgacctggc caatcgcttt cctgccgagc
     6661 tgtcgggggg cgaacagcag cgcgtgtcac tcgcgcgggc ggtcgtgaag aaccccgtcc
     6721 ttctgcttgc cgacgagccg accggttcgc tggacttcgc gacgtcgcgg gacatcctgc
     6781 gcctcctggt acgggtcaac gagacctacc acacaactat cgtgctcgtc acgcacaatg
     6841 ccgccatcgc agacatcaca gaccgcgtga tccggctgcg cagcggcgag gtcgaggagg
     6901 accgtctcgt cgggcgactc gtccaggcgg aggaggtgac ctggtgaaca cgctgtcgaa
     6961 gcgctttcct cgcatcgtcc gctccaaccg gtggctcgtg tatggcagcg tcacgctggt
     7021 catccttgcc tgcatgctgt atgccagcct caacgcactt gtcgtcgaca tcgacgccag
     7081 catggcgcag tttgagcgcg agtctcagat cgagtcggcc acgttcgttc cacagagacc
     7141 gtttgcagac ctgtctgtat tcgagagcga ccatgggttc gccatcgaac gccgtcgcga
     7201 ggtcgacgct gcgatggacg acggccttac gctgcgcgtg ttcgaacgga cgacgcatgt
     7261 ggacattccc gttatcaccg caggccgtga tctccaggca gacggcgaga tgctccttgg
     7321 tcagaccgcg gccacggccc tcaaacttcc cctggggacg acaatgactc tcctgggacg
     7381 ttcgttcacg gtcgttggat tcgccacagt cccggactat atgtacccta tcagaacgac
     7441 cgacaatgcc gacatcctcc ttgaccccaa ggcattcggc attgccgttg tcacccccac
     7501 tgcaatgaac gaccttgccc ccgaggctct cacaacctgg cacatcagga ctgtcgacgg
     7561 gacgacgatc gccgctcgac aggcactggt tgagacagtc ggtctcacat actggcagga
     7621 cgtcacggca aatccgcggt acacactcgc cacagccaag atcgaggggg cgacgtcggt
     7681 ctccacgagc atgccgctcg tcatcctgct gctcgcgtcg ctgctgctgg cggcggcaca
     7741 gggccgcatg gtacgcatgc agcggcccca gctgggcatg ctcaaggcgc tgggctattc
     7801 gacatgggaa ctggtgcgac aggagctggc ccttcctaca ttcattgcgc tgtcgggatc
     7861 agtcctgggc gtcctctcgt cgattctcac gatgcgcccc atgctgacct tcatggccag
     7921 ctacttcagt attccgccat atgtgacgac ggtcccgtgg aaagcggcag tcgtggccac
     7981 cgtggctccc atcctgttcg tagtcccagt cgtcgcgctg gttgcagtgc gtcttctcgg
     8041 acagtctccg gtcaagctca tgcagacgac cgccctgagc actcggccgg gtctcctgga
     8101 gcggtccttt catcttgtgc gaggctcgtt tcagaaccgg ttcgccgtca gagcggccgt
     8161 ccgcgctctg acacggttga cattgctgat gctcggggcc atgcttgctg gctacctgct
     8221 cctgttcggc ggcgcgatgc gtgactcgat gaatacgctg gtacaaggtg cattccagga
     8281 cgtgcggtac aactatacgt acatgttgac ggcaccacag ctcgggaacc cgtggggggg
     8341 agaggccctg acgatcgctc cgttcacggc gcatgacagc cagctgacgt tccaggtcat
     8401 ggggttgccc acgacctcca cactgttcct cccccgcgac agcacaggtc agccgatccc
     8461 gatcgatcgg gtcatcatta cgcgcgcact tgcacagaag ctcgacctgc agaccggtga
     8521 cagctggacg atgcagcagt cagctactgg acaagaggtt acgctgacgg tcgacgccgt
     8581 ggccgagatg tacacgacgg cagccgtcat aatgccccag gagcaactca acggcctcct
     8641 cggcctgcca ctcgggagtt tcaacgccct cctgagtaca acgagcttgc agatccctgc
     8701 tcgaaacgtg ctgatgactc tgtccatggc acagagccgc gctgccttcg actcaatcat
     8761 ggagccgctc tggatagggc tcggggctgt ggcgatcgtt gcagtgttca tcgcattgat
     8821 ggccctgtcc gtcgtgacga cgctgatggt agaggaagag cgggtgacca tctcactgct
     8881 caaggtcctt gggtatcgcc cggaggaact caacaggatg gtcctcgggt caggcatcgt
     8941 cgctgtcggg gcgggattcc tgatcagcat acctctgctt ctggcctctg tcgcgaagtt
     9001 gctgacgcga acgaccagca gcatcagctt cagcctgccg atccgactct cgcccctttc
     9061 gatcctggtt tgtgccgtga tcatgcttgc cacgtacctt gtctcattga gagctgcgcg
     9121 ccgcaaagtg ctgggcgtac cgatggcaat cagcctcaag gctgcgcgag agtgatggca
     9181 aaccctgcag agaacccttc ggccgatgcg aggaggagcg ccctgatcaa tgcggcgatc
     9241 agcgaatttg ccacgtccgg ctacctggct gcgtcgacga accgcatcgt ccgggcggcc
     9301 ggagtcagc
//
LOCUS       DATMZD010000066         2671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_133226,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000066 DATMZD010000000
VERSION     DATMZD010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133226"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..700)
                     /locus_tag="VN478_01380"
     CDS             complement(134..700)
                     /locus_tag="VN478_01380"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWQ21361.1"
                     /translation="MKRVAASLDEVIQSAIELVRREGLSSLTIRALSDATGVSVGTLY
                     NYFGDKTGLILRIMDSFWNTTLNVDVVRTLDKPLALPDMLEEVYAILRERSRLFHHDF
                     IRDVHMIEGLPAGHPGTRANYLMVVEGKLRDMIRRFPELDARIHERGAEDQLVQFLLD
                     NVMALLSRNAPDLGLFRDVLAAYLASLT"
     gene            complement(984..1338)
                     /gene="ssrA"
                     /locus_tag="VN478_01385"
     tmRNA           complement(984..1338)
                     /gene="ssrA"
                     /locus_tag="VN478_01385"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1453..>2671)
                     /locus_tag="VN478_01390"
     CDS             complement(1453..>2671)
                     /locus_tag="VN478_01390"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HWQ21362.1"
                     /translation="VEAARAWSSSEAARMLAELSTGYRLEKAKREAEVEKIRREGLES
                     ANERLRTVTRIGRSLTQSLEPRDILMRMWNELSTSVDLRVLGFGIYTAESGSIEFPGL
                     IESGVVQAASSVFLTDESSLAALCVREKRVLYYATSDDARSALGTSSLITFHREPSTV
                     QSILYLPLFREDEIVGVMTVQSANEHAYSEDVIEMLEAVASFAAIAVENARIMIRLNE
                     MNQVISGEKEQVEKAALASSWLAEHDSLTGLSNRRFLERILDENIRLATLENTSIAVF
                     FVDLDDFKKVNDTYGHDAGDRVLVAVGARLVSDFREGDYVARVGGDEFVVVAPGIRNV
                     DSVAAIADKVVASLDEPITIAEGPVSVTVSVGITLFPQHGRGSQELIARADEAMYSVK
                     RTGKGAWRTWSAF"
BASE COUNT          526 a          828 c          771 g          546 t
ORIGIN      
        1 cgcctcacga gggtcagccc cagggtgatg acggcgacca gatatgcaat atcaaaaatc
       61 gattccatga ttctttccat agttccttct cctgttgttg tctatgtttc agcacggcgg
      121 gcaggccaga cccttacgtc aggctggcca gatacgccgc gaggacatcc cggaacaacc
      181 ccagatccgg cgcgttgcgg ctcaggagtg ccatgacgtt gtccagcaga aactgcacga
      241 gctgatcctc cgctccgcgt tcgtgaatcc tggcgtcaag ctcggggaat ctgcggatca
      301 tgtcccgcaa cttgccctcc accaccatca ggtagttcgc ccgggtgccg gggtgccccg
      361 ccggcaagcc ctcgatcatg tggacgtcgc ggatgaagtc gtgatggaag agacggctgc
      421 gctcgcgcag gatcgcatac acctcctcca gcatgtcggg aagcgcaagc ggcttgtcga
      481 gggtccggac cacgtcgacg ttgagggtcg tgttccagaa tgagtccatg attctgagga
      541 tcaggcccgt cttgtcccca aagtagttgt acagggtccc gaccgagacg ccggtcgcat
      601 cggacagcgc acggatcgtg agggatgaca ggccttcacg gcgcaccagc tcgatcgcac
      661 tctggatgac ctcgtcgagg gaagcggcga ctctcttcat tgtcccgcct ccgcccgcca
      721 cggagtcttc tcccccgaga ccaggctttg cagctcaaca accgtgccaa gacttccttc
      781 cagagcttgc ttcatgtcga tcctccagca tacgtcgtga tatgaacgtt gttcaattga
      841 accatgttca ttatggagca ttgtccgtgg acgtcaatgt ggatccaagg gtgatgtgtg
      901 aaggaagagg acgcgtctgc ccgtgaggga gacaatgaaa aagcccactg gctggggtgc
      961 cggtgggcag gttgttctgt tcatggtgga ggtggcgaga gttgaactcg cgtccaaata
     1021 cgttaaacac agcgttacta caggtttagt cgaccattgt accttgcgct cggccgggag
     1081 tcgacgccca gctttgcacg atttcccgtt tggtactctc tggcaaggaa cgggaacgac
     1141 cctcgccttc gagaccccta tagacgcgcc cctgacggac ccagggatgg acggcccgga
     1201 ggaacgctag ccgcgcttag gcagctagag cgtaattgtt attggcttgt ttttttgtga
     1261 cagatgatta aagaggccaa ctgtcgtcct ctacctgcaa ccctgcacct cacgtatctg
     1321 tcgaacccca gcacccccag atacttctct cccgcaggag gggccaattc cctcgtgcag
     1381 aaggtgaagc acatcgatta taccacagat tgtccggccg ttcccgaaga caaggaaggt
     1441 ctgcccaccg ggctagaacg ccgaccacgt gcgccacgct cccttgcccg tccgcttgac
     1501 agagtacatc gcctcgtccg cacgggcgat cagctcctgg ctgccgcgtc cgtgctgcgg
     1561 aaacagggtg atgccgactg acacggtgac ggagaccggg ccctcagcga tggtgatcgg
     1621 ctcatcaagc gacgccacga ccttgtcggc gatggctgcc acgctgtcta cattccgtat
     1681 gccgggcgct acgaccacga actcatctcc cccgacgcgg gcgacgtagt ctccttcccg
     1741 gaagtcggag accaggcgtg cccccacggc cacgaggacg cgatcgccag cgtcatgccc
     1801 gtatgtatcg tttaccttct tgaagtcgtc gaggtcgacg aagaagacgg cgatgctggt
     1861 attctcgagc gtcgcaagcc gaatgttctc gtcgaggatt ctctcgagga agcgccggtt
     1921 ggacagcccc gtcaagctgt cgtgctctgc cagccacgaa gacgcgaggg cggccttctc
     1981 gacctgctcc ttctcgcctg agatgacctg gttcatctcg ttcagccgga tcataatgcg
     2041 cgcattctcg accgcgatcg cggcgaagga ggccactgcc tcgagcatct cgatgacatc
     2101 ttctgagtaa gcatgctcat tggcgctctg caccgtcatg acgccgacga tctcgtcctc
     2161 ccggaacaga gggagataga ggatgctctg gaccgtcgaa ggctcccgat ggaacgtgat
     2221 cagtgaactc gtgccgagtg ccgatcgtgc atcatccgag gttgcgtagt agaggacgcg
     2281 cttctctcgc acgcagagcg cagcaagact ggactcgtcg gtcaggaaga cggacgatgc
     2341 cgcctgtacg acaccggatt cgatcagccc ggggaactcg atggatcccg attctgcggt
     2401 gtagatgcca aatcccagca ccctgaggtc gaccgacgtc gagagctcgt tccacatccg
     2461 catgaggata tctctgggtt cgaggctctg cgtcagactc cgaccgatgc gtgtcacagt
     2521 ccggaggcgc tcatttgcag actcaagtcc ctcacgtcgg atcttttcga cctcagcttc
     2581 gcgcttcgct ttctcgagac ggtagccggt cgacaactcg gccagcatcc tggcggcctc
     2641 cgagctcgac catgcccgtg ccgcttccac c
//
LOCUS       DATMZD010000067         4624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_82644,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000067 DATMZD010000000
VERSION     DATMZD010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4624
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82644"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1518)
                     /locus_tag="VN478_01395"
     CDS             complement(<1..1518)
                     /locus_tag="VN478_01395"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HWQ21363.1"
                     /translation="MDQHKFVYDFEEGSKEMKTILGGKGAGLAEMTKIGLPVPPGFTI
                     STEMCPEYLKVGDIPETLRIEIREHLARLEGKLGKKFGSDQNPLLVSVRSGAAVSMPG
                     MMDTILNLGLNIETVEGLATLTGNPRFAWDSYRRFTQMYGNVVLGIEHNDFETILAEQ
                     KQLQGVTLDTDLSVDSLKALVQKYFALVQEKTGKPFPQDPMDQLLGAVGAVFRSWNTE
                     RAITYRKIEKISGLIGTAVTVQSMVFGNMGSDSATGVCFTRDNSTGAKRFSGEYLVNA
                     QGEDVVAGIRTPKNIDEMETEIPETYKRLVEVSLLLEKHYRDMQDMEFTVERGKLFML
                     QTRNAKRSPVAAVKVAVDMVGEGLLTKEEAVMRVTPTQLDTLLHPQVDPQAKVEVIAR
                     AIPASPGAGYGKVIFESKKAAELGEAGEKVVLVRQDTSPEDIDGISKAQATLTTRGGA
                     SAHAALVARGLGKPCVVGADQIKLNSGDKTFVANGVTVHEGDVITVNGTTGEVILG"
     gene            complement(1536..3611)
                     /gene="glyS"
                     /locus_tag="VN478_01400"
     CDS             complement(1536..3611)
                     /gene="glyS"
                     /locus_tag="VN478_01400"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HWQ21364.1"
                     /translation="MSDLLIEILTEELPARFVDNAQTQLMESVVAHLDEYFIQHGDVH
                     GFSTPRRLVVLAQNVKEQTDARTTEVKGPARASSYDAQGNPTPALLGFCRGQGVEPSD
                     AYIMDVAGRQYVYARKTIPGQRSSGLVEAFLPRLPRELTFPKMMHWEESGFQFARPIR
                     GLLALLGPEVLHWEVAGVVGSNTTYGLRVGKPKPIAVASPEDYMRKLREAFVIVDPAE
                     RRRLIEKQAEKLLKPLGYKLGDNMSDLLDEVVNLVEYPSAFLGDLPEIAAGLPESVVR
                     SVLQQQMHSFLLYDQNGNVVPHFLSVRNGLTDFIDIVRRGNESVANARLLDAAFFIQE
                     DMREPLEAYVGRLDTLIFMDKLGTMAAKTLRLERLADRAAEYSSLTSEEEATLTSAAH
                     LSKADLATSMVKEYTSLQGEIGSVYLSRAGGSPDVVSAIREQYVPRSPGEGIAATRVG
                     RLLGVVDKVDTLVGILSTGFNPSGSEDPYGLRRAGNGIVRTVVESRESVDLETLVSAG
                     IEAYRSTGSLPHPEELQQRVLDYLRGRVAQYFKEKQLTREYAALESLPIAELGSVPDR
                     MQALHEAAGDPALLTLSDSHRRIQNITKGLPASTSLPADLLLTDPEEVTLKQAAETTA
                     ARISLALERRDYLDAIHACEELSVPVTLFFDHILVMAPDERVRSARLLLLCYLKYILG
                     LVCDYSKLT"
     gene            complement(3604..4452)
                     /locus_tag="VN478_01405"
     CDS             complement(3604..4452)
                     /locus_tag="VN478_01405"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005979416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HWQ21365.1"
                     /translation="MNFEEMVSKLEHFWEDEGCTLLFPYDQEVGAGTLSPYTFLRVLG
                     RKPWKVVYVQPSRRPADGRYGENPNRLYMHHQLQVIVKPPTTDIQDTYLRSLYLLGLK
                     PAEHDIRFVEDNWETPVLGAAGVGWEVWLDGMEVTQFTYFQQAGGREVPVVAVEITYG
                     LERLAMFVQDKKNVYELEWHQGVTYGDLRRQVERENSVYSFEEADIEALVTMFDLCEK
                     ESQRMIDRKLITPAYEYLLKCSHTFNVLDARGTVGVSQRMAYLARVRRLAHGCADLYL
                     ENENHE"
     gene            complement(4442..>4624)
                     /locus_tag="VN478_01410"
     CDS             complement(4442..>4624)
                     /locus_tag="VN478_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21366.1"
                     /translation="GEGSIDGRHTLMGIALALRLLRSAAGRLAITSAFERFAQNAATL
                     FHSDTREQLEESCHEL"
BASE COUNT          903 a         1330 c         1364 g         1027 t
ORIGIN      
        1 gcccaggatg acttcaccgg ttgtgccgtt caccgtgatg acgtcgccct cgtggacggt
       61 cacgccgttt gcgacaaagg tcttgtcgcc actgttgagc ttgatctgat cagcaccgac
      121 cacacacggc ttgccaagac cacgtgccac aagcgctgca tgggctgagg cgccaccgcg
      181 cgtcgtcagc gttgcctgag ccttggagat gccatcgatg tcttcaggag acgtgtcctg
      241 acgaacgagc acgaccttct ctccggcctc tcccagctcg gctgccttct tcgactcaaa
      301 gatgaccttt ccatagccgg cgcctgggga ggcggggatt gccctggcga tcacctcaac
      361 cttggcttgt gggtctacct gaggatggag cagggtatcg agctgagtag gagtcacacg
      421 catcacagcc tcttccttgg tgaggagacc ttcgcccacc atgtcgactg caaccttgac
      481 agccgcgacg gggctccgct tggcattccg cgtctgaagc atgaacagct tgccgcgttc
      541 gacggtgaac tccatgtcct gcatgtcgcg atagtgcttc tcgaggagaa gcgacacctc
      601 gacgaggcgc ttgtacgtct cgggtatctc agtctccatc tcatcgatgt tctttggcgt
      661 gcgaatgccg gctacgacgt cttcgccctg agcgttgacg agatactcgc cggagaaccg
      721 cttggctccg gtggaattgt cacgggtgaa acagacgccg gttgcggagt cgctgcccat
      781 gttgccgaac accatggact ggacagtgac cgcggtaccg atgaggccac tgatcttctc
      841 gatcttgcga taggtgattg ctcgctcggt attccatgag cggaaaaccg cgccaacggc
      901 tccgagcagc tgatccatcg gatcctgcgg gaagggcttg cccgtcttct cctgaacgag
      961 agcgaagtac ttctgaacca gggccttcag gctgtcgacg gacaggtccg tatcaagtgt
     1021 gacgccctgg agctgcttct gctctgcaag tatggtctcg aagtcattgt gctcgatgcc
     1081 gagcacgacg ttgccgtaca tttgagtgaa cctgcgatac gaatcccagg caaagcgtgg
     1141 gttcccagtc agtgtcgcaa gcccttcgac agtctcgatg ttgagtccga ggttgaggat
     1201 agtatccatc atgccgggca tcgagactgc tgcacccgaa cggacggaca cgaggagtgg
     1261 attctggtca gagccaaact tcttgcccag cttgccctcc aatctggcaa ggtgttcacg
     1321 gatctcaatc ctcagagtct caggaatgtc accgacctta aggtactcgg gacacatttc
     1381 ggtggagatt gtgaaaccgg gaggcacagg aaggccgatt tttgtcatct ctgcgaggcc
     1441 tgctcccttt cctccgagga tggtcttcat ctccttgctg ccttcttcga aatcgtagac
     1501 aaacttgtgt tgatccatgg ttcctcctgt ccggcctagg ttagcttgga atagtcgcaa
     1561 acaagcccaa gtatatactt gaggtagcac aaaagcaata aacgtgcgct tcgcacgcgt
     1621 tcatcgggag ccatcacgag gatatggtcg aagaagagag tgacgggaac ggagagttct
     1681 tcacacgcgt ggatggcatc caggtagtct cgtcgctcga gagcgaggga gatacgcgcc
     1741 gcggttgtct cggccgcctg tttgagtgtg acctcttccg ggtctgtcag aagcaggtca
     1801 gcgggcaggc tcgtcgaagc ggggagtccc ttcgtgatat tctgtattct gcggtgactg
     1861 tctgagaggg tcagtagcgc agggtcgcct gctgcttcgt gaagggcctg catgcggtcg
     1921 ggaacagaac cgagctcggc aattggcagg gactcgagtg ctgcgtactc cctggtcaat
     1981 tgcttctcct tgaagtactg cgccacgcgt cctctgagat agtccaggac cctctgctgc
     2041 agctcttcgg gatgaggcag cgaacccgtg gaccgatatg cctctatccc tgcactcacg
     2101 agggtctcca agtccacgct ctcgcgcgac tctactactg tgcggacgat gccgttgcct
     2161 gcacgcctga gaccgtaagg gtcttcagat ccggacgggt tgaaacctgt gctcaggatg
     2221 cctacgagcg tgtcgacttt gtcgacaact cccagcaggc gtccgactct tgtcgctgca
     2281 atgccttcgc cgggagaccg gggcacgtac tgttcccgga ttgccgaaac gacatccggt
     2341 gatccgccgg cacgtgacag atagacactg ccaatttcgc cttggagcga cgtatactcc
     2401 ttcaccatgc tggtagcgag gtcggccttg gagaggtgtg ctgcgctcgt gagcgtcgcc
     2461 tcctcttcag acgtcagaga ggagtactcg gctgcgcggt ctgccaatcg ctccagtcga
     2521 agagtcttgg ccgccatggt gccgagcttg tccatgaaga tcagagtgtc cagtcggccc
     2581 acgtatgcct ccagaggctc acgcatgtcc tcctggatga agaaggccgc atcgagaaga
     2641 cgggcgttgg cgacgctctc gttgccccga cgcacgatgt cgatgaagtc agtgagccca
     2701 ttgcgcacgc tcaagaaatg tggcacaacg ttgccgttct ggtcgtaaag caggaacgag
     2761 tgcatctgtt gctgcagcac ggaacggacg acgctctcgg ggagtcctgc agctatctct
     2821 gggaggtccc ccagaaaggc ggatgggtac tcgacaaggt tcacaacctc atcgagcaga
     2881 tcactcatgt tgtccccaag cttgtagcca agcggtttca gaagcttttc ggcctgtttc
     2941 tcgatcaggc gacggcgctc ggcagggtcc acgatgacga atgcttcgcg aagctttcgc
     3001 atgtagtctt cgggcgacgc cactgctatc ggctttggct ttccgacgcg gagaccgtat
     3061 gtggtgtttg agcccacgac tccggccact tcccagtgaa ggacttccgg gccaagcagt
     3121 gcgagcaacc cacgaatggg ccgcgcaaac tggaatccag actcctccca atgcatcatc
     3181 ttcgggaagg tgagctcgcg ggggagtcgt gggagaaagg cctcgacgag tcccgaggat
     3241 ctctgaccgg ggatcgtctt cctggcgtag acatactgtc tgcctgccac atccatgatg
     3301 taggcatcgg agggctctac tccctgtcct cggcagaagc cgaggagtgc cggcgtgggg
     3361 tttccctgag cgtcatagct ggacgctctg gccgggccct tgacttccgt cgtacgtgca
     3421 tccgtttgtt ccttgacgtt ctgggccagg acgacaagcc gtcgtggcgt ggagaagccg
     3481 tgcacatcgc cgtgctggat gaagtactcg tcgaggtgtg ccacgacgct ctccatgagc
     3541 tgtgtctgag cattgtccac gaaacgggcc ggaagctcct cggtcaggat ctcgatcagc
     3601 aggtcactca tggttttcat tctccagata gaggtctgca cagccatgtg caagacggcg
     3661 cactcgggca aggtaggcca tacgttgtga cacgccaaca gtaccgcggg catcaagaac
     3721 attgaaggtg tgtgagcatt tgagaagata ctcatatgca ggagtgatca gcttccggtc
     3781 gatcatgcgt tgagactcct tctcgcagag gtcgaacatc gtgacgagcg cctcaatgtc
     3841 ggcttcttca aatgagtaga cgctgttctc cctctcgact tgccgtcgaa ggtccccata
     3901 ggtgacccct tgatgccact ccagttcgta cacgttcttt ttgtcctgaa cgaacatggc
     3961 aagccgttcg agaccgtagg ttatttcgac tgcaacgaca gggacctccc tccctcccgc
     4021 ctgctgaaag tacgtgaact gagtcacttc catgccgtcg agccacactt cccacccgac
     4081 gcctgctgct ccgaggacgg gtgtctccca gttgtcctcg acgaatcgga tgtcgtgttc
     4141 ggcaggcttg aggccgagca ggtagaggct tcgtagatac gtgtcctgga tgtccgtggt
     4201 gggaggcttc acgatcacct gcaattgatg atgcatgtag agacggttgg ggttctcgcc
     4261 atagcggccg tctgcagggc gtcgtgacgg ctgaacgtag acgactttcc agggcttgcg
     4321 ccccaggacg cgaaggaacg tgtagggact cagtgttcca gctcctacct cctgatcgta
     4381 agggaacagg agggtgcagc cctcatcctc ccagaagtgc tccagcttgg aaaccatttc
     4441 ttcaaagttc atggcatgac tcctcgagtt gttctcgtgt atcagagtga aacagtgtag
     4501 cagcgttctg cgcgaaccgt tcgaaggctg atgtgatggc cagcctgccg gccgcggacc
     4561 tcagtagtcg caatgcaagc gcgatgccca tgagtgtgtg gcgtccgtcg atactccctt
     4621 cccc
//
LOCUS       DATMZD010000068         2594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_52416,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000068 DATMZD010000000
VERSION     DATMZD010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52416"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..155
                     /locus_tag="VN478_01415"
     CDS             <1..155
                     /locus_tag="VN478_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012971299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HWQ21367.1"
                     /translation="GICGEQGGEPDSIDFCHTSGLDYVSCSAPRVPIARLSAAQAAIR
                     NRPTAN"
     gene            172..1278
                     /locus_tag="VN478_01420"
     CDS             172..1278
                     /locus_tag="VN478_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HWQ21368.1"
                     /translation="MLPITNYRDREGRLLSQGAAFGIASKGRAVPESDDGIRSPFQKD
                     RDRIIHSRAFRRLQYKTQVFIAPKGDEIRTRLTHTLEVAQLSRSVSDALGLNEDLVEA
                     IALGHDLGHAPFGHTGEAVLDKKLKQIDPSLGFEHAQQSLRVVDSLERRERGYGDVLF
                     GLNLTYETRNGIACHSKGLQEMGNLEDMLSPQTLEGQIVRLCDRVAYVNHDLDDAIHA
                     GIIDVADVPALTVSALGSFYSQRLDTMVLDIVRTSSVAGEIAMSGPVQGAMDDLMLFL
                     KDRVYMHSEPKREESKACDLLSRIFDFCMQDIRRMEPYLREHPMVCESDDPVTMLHGD
                     LHSTARIVTDYIASMTDRMALKVATDLVMPHIYV"
     gene            1361..>2594
                     /gene="dnaG"
                     /locus_tag="VN478_01425"
     CDS             1361..>2594
                     /gene="dnaG"
                     /locus_tag="VN478_01425"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HWQ21369.1"
                     /translation="MEYKEYIADIGTKIGILDVVGHYVRLRKQGRNYVGLCPFHHEKT
                     PSFTVSPDKNMFYCFGCKASGDMLTFVRKVHNVSLPEAIEIVSKEFHVELPPWHMSTA
                     ASRERQELLQTLKLVATAFAAALQNPQARECQEYVKSRALSSETVNQFSLGYCPRDVD
                     TLVKWCSEHGIDEDCLARTGIVQRHEGHSFSPFAHRLMFPIWDSIGNIIAFGGRAIDG
                     SMPKYTNSPETPLFAKRRTLYALPLAEASIRRTGTAVVVEGYMDAIALHAAGFTNTVA
                     SLGTAFTEEQGILLKRSASRVLLNFDSDEAGQRAARLALSVADRTGLDVAVVRVEGAK
                     DPDELLREPGGRAQYQAALDRAISVGDFLLNAAVAGRDLNSVSDRRAFVQEMMESVDD
                     MQDSLLRTQLLTQIAHRVG"
BASE COUNT          569 a          752 c          765 g          508 t
ORIGIN      
        1 tcggtatctg cggcgaacaa ggtggtgaac ccgattcgat cgacttctgc cacacatccg
       61 ggctggacta cgtttcgtgc tcggcacccc gagtccccat tgcacgcctg tcggctgcgc
      121 aggccgccat caggaacagg cccaccgcca actagacgcg agtcctcata catgctacct
      181 atcacgaact acagggatag ggaaggaagg ctgctctctc agggagcagc ctttggcata
      241 gccagcaagg ggagggcagt gcccgagagc gatgacggta tccggtctcc gttccagaaa
      301 gaccgcgacc gcatcatcca ttcgagggcg tttcgtcggc tccagtacaa gacacaggtg
      361 ttcatagcgc ccaagggcga cgagatacgc acgaggctca cgcataccct cgaggttgcc
      421 cagctgtcgc ggtcagtatc tgacgctctc ggtctcaacg aggacctcgt cgaggcgatc
      481 gcgctagggc atgaccttgg ccacgcgcca ttcgggcaca cgggcgaggc agtcctggac
      541 aagaagctga agcagatcga tcccagcctg ggattcgaac atgcgcagca gagccttcgc
      601 gtggtcgaca gtctggagcg ccgtgagcgt ggatacggcg atgtgctctt cggtctcaat
      661 ctcacctatg agacccgcaa cgggatcgcc tgtcacagca agggactcca ggagatgggc
      721 aatcttgagg acatgctgag cccgcagact ctggaggggc agatcgtgcg actctgcgac
      781 agggtagctt atgtcaacca cgacctcgac gatgccatcc atgcgggaat cattgatgtg
      841 gctgacgtcc cggcgttgac ggtgagtgca ctcggcagct tctactcaca acgcctcgac
      901 acgatggtgc tggacatcgt ccggactagt tcagttgcgg gcgagattgc catgagtgga
      961 ccagttcagg gtgccatgga cgatctgatg ctgttcttga aggacagggt ctacatgcat
     1021 tcggagccca aacgcgaaga gagcaaggca tgcgatctgc tgtcgcggat cttcgacttc
     1081 tgcatgcagg acatcaggcg catggaaccc tacctgcgag aacaccccat ggtctgcgag
     1141 tcggatgatc ccgtcacaat gctgcatgga gatctgcata gcacggcacg cattgtgacg
     1201 gactacattg cctcgatgac cgaccgaatg gcgctcaagg tagcgacgga cctagtcatg
     1261 ccacacatct atgtctaggt agcccaaggg agccgattgc ggcgtatgtg tgaggaggcc
     1321 gggaacgatt ccggcctcct cactcgtata cctatttggc atggaataca aagagtacat
     1381 cgcagacata ggcaccaaga tcggaatcct tgacgtcgtc ggccactacg tgagactgcg
     1441 caagcaaggg cgcaactacg ttggtctctg cccttttcac catgagaaga ctccttcatt
     1501 cacggtcagc cccgacaaga acatgttcta ttgctttggc tgcaaagcat ccggagacat
     1561 gctgacgttt gtccgcaagg tacacaacgt gtcgcttccg gaagcaatcg agatcgtatc
     1621 caaggagttt cacgtcgagc tgcctccctg gcacatgtcg acagccgcat cacgggaacg
     1681 tcaggagctg ctgcagacgc tcaagctcgt ggcgacggct tttgccgccg cgcttcagaa
     1741 ccctcaggca cgcgagtgtc aggaatatgt caaaagccgc gccctcagtt ccgagacggt
     1801 caatcaattc tctcttgggt attgtccgcg cgacgtggac acactggtca agtggtgctc
     1861 cgaacacggg atagacgagg actgtcttgc acggactggt atcgtgcaac ggcacgaagg
     1921 gcattcattc tcaccatttg cccacaggct gatgttccct atctgggaca gcatcggcaa
     1981 catcatcgca ttcggtgggc gagctatcga cgggtcgatg ccgaagtaca cgaatagccc
     2041 ggagactccg ctgtttgcga agagaagaac cctctacgca ctgcctcttg ctgaggcatc
     2101 gatccggaga accggaactg ccgtggtcgt cgagggctac atggacgcta tcgccctcca
     2161 cgctgccggc tttaccaaca ccgtggcctc actcggcacg gccttcacgg aagagcaggg
     2221 cattctgctg aagcggagcg cctctcgcgt actactcaat ttcgacagtg acgaagcagg
     2281 tcagcgcgcg gcccgactcg cactctccgt tgccgatcga accgggttgg acgtcgctgt
     2341 cgtcagagtc gagggtgcaa aagacccgga tgagttgctg cgtgaacccg gaggaagggc
     2401 ccagtatcag gctgctctcg atcgggcgat ctcagtcggc gactttctcc tcaatgctgc
     2461 tgtggcgggc agggacctga attctgtcag cgacagaaga gcgtttgtgc aagagatgat
     2521 ggaatcggtg gacgacatgc aggactcgct gctcagaaca cagctcctca cgcagattgc
     2581 acacagggtt ggcg
//
LOCUS       DATMZD010000069         3160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_62405,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000069 DATMZD010000000
VERSION     DATMZD010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62405"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /gene="wecB"
                     /locus_tag="VN478_01430"
     CDS             <1..772
                     /gene="wecB"
                     /locus_tag="VN478_01430"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HWQ21370.1"
                     /translation="VEAGLRSHRKFSPFPEEINRTLTDQVSDLCFAPTEEARLNLLHA
                     GIDMSSIHVVGNSIVDAVHMALPHVHAPHIMAKVRPDSPMVIMTAHRRENWDTGIATF
                     SDALVELSSLHPELTFVFPVHLNPVVRETVFGIASGLPNVVLTDPLPYFDFLYMLEHC
                     VAVLSDSGGIQEESVTLHKPMLLLREVTERPEAVASGWVKLVGQDKSLIVSSFEELAA
                     GGFAGPGAHESNPYGDGTTAAQIVSCIRSYLNATHSGG"
     gene            776..2053
                     /gene="murA"
                     /locus_tag="VN478_01435"
     CDS             776..2053
                     /gene="murA"
                     /locus_tag="VN478_01435"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HWQ21371.1"
                     /translation="MTTEQSFLIEGGNSLHGSVRCSGAKNSILPLVAAAIAVRDIVSF
                     QNVPDISDLQSLLALIEAMGVKLVSFTNGELVLDTRGPVETRVENTNGYSLRGTQTLV
                     GALLAREHKAMLPSLGGCSIGARPIDLHIKGLRAMGTQFEWKDGYLIGQAAALKACDV
                     YLDFPSVGATENLLLAAALAEGTTRVFNAAQEPEVYDLISFLNKAGARIVPVFPFGFR
                     VEGVSQLHGASHRIIGDRIEASTLMVATAITQGEATITGIDARHIWSIIAKLRETGAT
                     VEVEGDDAVVVKGVSEYRSTDIRTLTFPGYPTDAQPQMTAYLTLARGNSVVVESIFEN
                     RFGAILELERMGARIKVAEETAIIEGVQSLNSATVQAKDLRGGAALVLAGLAAHGTTT
                     VKSIHHIDRGYESLESKLFQLGAHVTRVTQPEA"
     gene            2057..2407
                     /locus_tag="VN478_01440"
     CDS             2057..2407
                     /locus_tag="VN478_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020266870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HWQ21372.1"
                     /translation="MIEREQILDALREVYDPEIPINVVDLGLIYGVEVTADGDVTVRM
                     TMTAPQCPMSGYLLQQAEQGVRTVPGVRDVKVDLVFDPPWEPSMIKEDALKSAGIVTD
                     APADMAGSDCPPEK"
     gene            2404..>3160
                     /locus_tag="VN478_01445"
     CDS             2404..>3160
                     /locus_tag="VN478_01445"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HWQ21373.1"
                     /translation="MKEIAPRQPAGALAANLDQLIESFLLAQDVGNASKIVYRRALKR
                     FKEFLTLREAAGGVGYLTKTDIVQYKSHLLAEGLSPRTGNLYLTSVRQFFKYLESSRI
                     YPDVSAGLKGFKRSYGFNRDPLTLQQARDLLSSIGTSDMIGLRDFAMINLMLRTGLRT
                     MEVVGSDVGDIRQTSNATLLYVRSKGKDAKDDFVVLTREAYEPIARYLAARHCTDPRA
                     ALFVSHSNNNDGRRITTKTLRFQVKERLLRIGVY"
BASE COUNT          664 a          920 c          937 g          639 t
ORIGIN      
        1 cgtcgaggca gggctgaggt ctcaccgaaa gttcagccca tttcccgagg agatcaaccg
       61 cacgcttaca gatcaggttt ccgacctctg ctttgcaccc actgaggaag ctaggctgaa
      121 ccttctgcat gcaggcatcg acatgagcag catccatgtt gtcggaaact cgatcgtaga
      181 cgctgttcac atggctcttc cgcacgttca tgctccgcac atcatggcga aggtccgccc
      241 tgacagtccc atggtcatta tgacagcaca tcggcgcgag aactgggata cgggcattgc
      301 caccttctcg gacgcgctcg tagagctgtc cagtctgcat cccgaactga cgtttgtctt
      361 cccggtccac ctcaacccag tcgttcgaga gacggtgttt gggatcgcct cgggtctgcc
      421 caacgtcgtg ctcactgacc cacttcccta ctttgacttc ctgtacatgc ttgaacactg
      481 cgtggcagta ctgagcgatt cgggcggcat tcaggaggaa tcggtgacac tgcacaagcc
      541 catgctcctg cttcgcgaag tcacagagcg tccagaggcc gtggcaagcg gctgggtgaa
      601 gctggtcggt caggacaaga gccttatcgt ttcatcgttt gaagaacttg cggcaggcgg
      661 gtttgcaggc ccgggcgcac atgagtccaa tccatatggg gatgggacca ctgcagcaca
      721 gatagtctcc tgcattcgca gctatctcaa tgcaactcac tcaggaggat aacgcatgac
      781 cacggaacag agctttctca tcgagggagg gaactcgctt cacgggtcgg ttcgctgcag
      841 tggagcaaag aactccatct tgccacttgt tgcagctgca attgccgtcc gcgacatcgt
      901 gagcttccag aatgttccag acatcagcga cctccagtcc cttctcgccc tgatagaggc
      961 tatgggtgtg aaactcgttt cgttcacgaa tggggagctc gtacttgaca ctcgtggccc
     1021 cgtcgagacg cgcgtagaga acaccaacgg gtacagtctg cgggggacgc agacactggt
     1081 cggagcgctc ttggctcgtg agcacaaggc aatgctgccc tcgctcggcg gctgcagtat
     1141 tggcgctcgc cccatcgatc tccacatcaa ggggctgcga gccatgggaa cgcagttcga
     1201 gtggaaagac ggctacttga tcggtcaggc tgcagcgctc aaggcatgcg acgtctacct
     1261 cgacttcccc agcgtcgggg cgaccgagaa cctcctgttg gccgccgctc ttgccgaagg
     1321 gaccactcgt gtcttcaatg cggctcagga gcctgaggtg tatgatctca tctcattcct
     1381 caacaaagca ggcgcccgca tcgtcccggt ctttccgttc ggatttcgtg tcgagggggt
     1441 ctctcaattg catggagctt cacaccgcat cattggcgac cgcatcgaag cttcgacact
     1501 catggtggcg acagccatta cgcagggtga ggccaccatt acgggcatcg acgctcgcca
     1561 catctggtcc atcatcgcca agttgcgaga gacgggggcg acggttgagg tcgagggtga
     1621 tgatgccgta gtcgtgaagg gggtctcaga gtaccgttcg acggacattc gtacgctgac
     1681 gttcccagga tatcctaccg acgcccagcc tcagatgacg gcatacctga cgttggcacg
     1741 gggaaactcc gtggtggttg agagcatctt cgagaaccga tttggtgcca tcctggaact
     1801 cgagcgtatg ggtgctcgca tcaaggtggc ggaagagaca gccattatcg aaggggtgca
     1861 gtcattgaac tccgcgacgg tccaggcaaa ggaccttcgc ggtggcgccg ccctcgtgct
     1921 cgctggcctt gcagcgcacg gcacgacgac ggtgaagtcg atccatcaca tcgaccgagg
     1981 ttacgaatcg ctggagagca agctgtttca actcggcgca cacgtgactc gcgtcactca
     2041 accggaggca tagaccatga tagagcgaga acagatcctc gacgccctgc gagaggtgta
     2101 cgaccccgag atcccgatca acgtggtgga tcttgggctg atctacggag tagaggtcac
     2161 tgcggatggc gatgtcacgg tccggatgac catgacagcc cctcagtgcc cgatgagcgg
     2221 atatttgctc cagcaggccg aacagggtgt tcgcactgta cccggggtcc gggacgtcaa
     2281 ggtggatctc gtattcgatc cgccctggga gccttccatg atcaaggaag acgcactcaa
     2341 gagtgctggt atcgtcactg acgctccggc tgacatggcc ggaagtgact gcccccctga
     2401 aaaatgaagg agattgcgcc gcggcaacct gcgggagcgc ttgcggcgaa tcttgaccag
     2461 ctgatcgaga gcttcctgtt ggctcaggac gttggcaatg ccagcaagat cgtgtaccgg
     2521 cgggcactca agcgcttcaa ggagttcctg acgcttcgag aggccgctgg cggggtcggg
     2581 tatctgacga agacggacat tgtccagtac aagagtcatt tgctggcaga ggggctgtcg
     2641 ccccggacgg gaaacttgta cctgacatcc gtccgccagt tcttcaagta tctcgagtcg
     2701 agccgcatat acccggacgt gtccgcgggt ctcaaagggt tcaaacggtc gtacgggttc
     2761 aaccgtgacc cgctcaccct gcaacaggca cgcgacctgc tgagctcgat cggtacgagc
     2821 gacatgatcg ggctgcgcga ctttgccatg atcaacctga tgctccgcac tggccttcgc
     2881 acaatggagg tggtcgggtc cgacgtcgga gacatacgtc agacatccaa cgcgacgctt
     2941 ctctacgttc gcagcaaggg gaaggacgcg aaagacgatt tcgtcgtctt gacgcgagaa
     3001 gcctatgagc caatcgcccg gtatctcgca gcgcgacact gcaccgaccc gcgagcggcg
     3061 ctgtttgtgt cacacagcaa caacaacgac ggtagacgca taaccacgaa gactcttcga
     3121 tttcaggtga aggagaggct cctccggatc ggtgtgtact
//
LOCUS       DATMZD010000070         2084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_103130,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000070 DATMZD010000000
VERSION     DATMZD010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103130"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..920
                     /locus_tag="VN478_01450"
     CDS             <1..920
                     /locus_tag="VN478_01450"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HWQ21374.1"
                     /translation="FDYYFKKYGIPEAGFSPDAEFPIVNGEKGILNLQLVGHGARGGI
                     LRHAEGGTRSNVVPQHAEALLVTDHDKLQRLQGLIDRLPKDEFLVSAALANGELKIDV
                     EGMPAHASTPEKGRNAIGKLLQVLDAVHCLEDDESLQFLAREIGTEWTGHRVGVDFAD
                     EVSGVLTLNLGTLHVDHDDSTAVINIRYPIKVTSKQVTDQFVEKLLGTKVEFSVMSDS
                     TPHYVDPGTPLIQTLQKAYTEYTGTEAKCFAIGGGTYARAIPGAVAFGPLFPGQPMTE
                     HGPDEYIEVDSLLKATRIYAYAMTELLKG"
     gene            922..1563
                     /locus_tag="VN478_01455"
     CDS             922..1563
                     /locus_tag="VN478_01455"
                     /inference="COORDINATES: protein motif:HMM:NF014038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HWQ21375.1"
                     /translation="MGTEREVKYQVLDQALFRRLNAMDHIGNFALEHAGTVDILTIYL
                     DTPELSLFYGHHALRLRIMQGRVFLSLKGPSTSSSALVQERPEDEVDAPGFSEKTLPG
                     TEDILRLMPSSAELLKGMTLSTSLRSMDKRSLHYVLHETERAYEIACDTVVFSSPRKP
                     GVKADATELEIEQKAGSVEGLQDLTRAFETFFAVVPRRGSSKYQDGLKALELI"
     gene            complement(1625..>2084)
                     /locus_tag="VN478_01460"
     CDS             complement(1625..>2084)
                     /locus_tag="VN478_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21376.1"
                     /translation="SGASLDVKSTPGVISVALTVAREVSGQQPSGAAVESPQTTDLKG
                     KRVLVIGKPSPLAMMLGATGCTAQAVSTWDDAAGGIAGFAPQVILAVVGDSPDLDAAV
                     EGRKRLGLPVILLCQQGVGCPADKASSVDGVLGLPLDLDDLRRMLTHILH"
BASE COUNT          434 a          686 c          592 g          372 t
ORIGIN      
        1 actttgacta ctacttcaag aagtacggca tccccgaggc cggattcagc ccggatgcag
       61 agtttccgat cgtcaacggc gagaagggca tcctcaacct gcaactggtc ggccatggcg
      121 cgcgcggagg catcctgcgt cacgccgaag gcggcacgcg ctccaacgtc gttccacagc
      181 atgccgaagc gctcctggtt accgatcatg acaagctgca gcgcctgcag gggctgatcg
      241 atcgcctgcc caaggacgag ttcctggtat cggcggcgct tgcaaacggc gagctcaaga
      301 tcgacgtgga aggcatgcct gctcatgcgt ctacccctga gaagggccgc aacgccatcg
      361 gcaagctgtt gcaggtgctc gatgccgttc actgcctgga ggacgacgag tccctgcagt
      421 tcctggcacg cgagatcggt accgaatgga cgggacatcg cgtgggcgtc gacttcgctg
      481 atgaggtctc gggcgtcctc acgctcaacc tgggcaccct gcatgtcgac catgacgaca
      541 gcaccgccgt catcaacatc cgatatccca tcaaggtcac cagcaagcag gtcaccgacc
      601 aattcgtcga gaagctgctg gggaccaagg tcgagttctc ggtcatgtca gacagcacgc
      661 cgcactacgt cgaccccggc acacccctca tccagaccct gcagaaggcg tatacagagt
      721 acacaggcac ggaggcgaag tgcttcgcca tcggcggcgg cacgtacgcg cgggctatcc
      781 ccggcgcagt cgcctttgga ccactgttcc cgggccagcc gatgactgaa catggacccg
      841 acgagtacat cgaagtcgac tcactgctca aggcgacgcg catctacgca tacgctatga
      901 cagaactcct gaagggatag ggtgggcacc gagcgcgaag tcaagtacca ggtcctcgat
      961 caggcgctgt ttcgccgcct gaacgccatg gaccacattg ggaacttcgc gctcgagcac
     1021 gccgggaccg tcgacatcct gaccatctac ctcgacactc ccgaactatc cctgttctac
     1081 ggccaccacg ccctgcgcct gcgcatcatg cagggcaggg tctttctttc tctcaagggt
     1141 ccatccacct catctagcgc gctggtgcaa gagcgccctg aggacgaagt cgatgcaccg
     1201 gggttctcgg agaagaccct tccgggcaca gaggatatcc tgcgtctgat gccgtcgtcg
     1261 gctgagctgc tcaagggcat gactctgtcc acttctctcc gatcaatgga caagcgctcg
     1321 ttgcactacg tcctccacga gactgagcgc gcctacgaga tcgcctgtga cacagtcgtc
     1381 ttctcttcgc cccgcaagcc cggcgtcaag gcagacgcga ctgaactgga gatcgaacag
     1441 aaagcgggtt ccgtcgaagg ccttcaggac ctgacgcggg cattcgagac gttcttcgcc
     1501 gtcgtcccgc gccgtggatc atcgaagtac caggatggcc tcaaggcact cgaactcatc
     1561 tagttcgtgt cttgtcatca attgtgaatc cctttgtgcc tgaccccaag ggattcacaa
     1621 ttggtcagtg aaggatgtga gtgagcatac gacgcaggtc atcgaggtcg agaggcagac
     1681 ccagaacgcc atcgacggac gaagccttgt cagcgggaca accgacaccc tgttggcata
     1741 ggagaatgac cggtaggccc agacgcttgc ggccttccac cgccgcatcc aggtcaggac
     1801 tgtcaccgac gaccgccagg atcacctggg gagcaaatcc cgcgatgccc cccgctgcgt
     1861 catcccaggt cgacacagcc tgagccgtac accccgtcgc ccccagcatc atcgcaagtg
     1921 gcgaaggctt gcctatgacc agcacacgct tccccttcag gtccgttgtc tgcggcgact
     1981 caacggcagc accggagggt tgctgcccac tgacttctct cgccacggtc aacgcaacgc
     2041 tgatgacacc aggggtggac ttgacgtcaa gcgacgcccc gctc
//
LOCUS       DATMZD010000071         3965 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_82793,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000071 DATMZD010000000
VERSION     DATMZD010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3965
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82793"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="VN478_01465"
     CDS             <1..414
                     /locus_tag="VN478_01465"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HWQ21377.1"
                     /translation="DYDAKYKVGYKTTAGTESGVQKAEARKVPANLGEDLTHQVQDLA
                     CRAFTALDCRGVARVDFLIDESGTVYVNEINTIPGALSFYLWEAVGMQYRDLLTKLVD
                     LACAAFEDRRSNIVSYDVSDILRMGSKGKSGTRKA"
     gene            424..1209
                     /locus_tag="VN478_01470"
     CDS             424..1209
                     /locus_tag="VN478_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWQ21378.1"
                     /translation="MATTLVDGITLAYVDEGFGDPVVLLHGWGGQAASMTPLITGLRQ
                     QYRIVAFDLPGFGGSSPPPVPWGTPEYAAFVEKAVASLGIARATYVGHSFGGRIALWL
                     SSHRPEIVQALVLVDAAGIKPPTTVKRRMRLIVYKTARMLLRLPILGAKGPALQERLA
                     MRFGSADYKAVSGVMRSSFVKTVNLDLSDCMHTITAPTLLIWGEKDQATPITDGRTME
                     RLIQGSRLIAVAGAGHFSYLDSPSFVNAVVSAFLHGTREGQVS"
     gene            1206..2813
                     /gene="murF"
                     /locus_tag="VN478_01475"
     CDS             1206..2813
                     /gene="murF"
                     /locus_tag="VN478_01475"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HWQ21379.1"
                     /translation="MKPLTTSVLAIAALMCLALTGVRARYLLHMLQLEEYDPRRFFHW
                     VVGHSKRLYGNYPLDALGVMGLAIVIVHPSRPVVAAVSGGMAVALCVGGLLQEFRWMK
                     APAKKALVMTPRAWRLLACCLIIGAAASVVPFVSSRWWDLAASLLLPLAAPLLMLLSR
                     FIMEPVEASIRDWYVRDARGILAGFAPTVVGVTGSYGKTSTKFFTAHLLESRYRVLMT
                     PESYNTAMGICRVIRGELGPEHEVFVVELAENERGGFGHLLRLAPCSISIVTSVGLQH
                     LEEFGSEAAIRRVMQGFVRMPGSGPTVVLNGDDPVLSDIQGVPGKRLLRASASGSLSS
                     DLRAENVAMGADGLSFDIVTSQGERFAYHTTVLGRHNVENILLAVAVAHDLGVSWSSI
                     QDGISTLQAPPHRLQMMLGAGGVRIIDDAFNSNPAGFAMAMEVLAGFSSRRVLVTPGL
                     VSLGTAEDAANVEAGRQAARAADTVILVGPAKTRPVREGLVSAGFPETSIFTARSLEE
                     VTALMRSLLEPGDTVMFENDLPDTYDE"
     gene            2992..3543
                     /gene="dcd"
                     /locus_tag="VN478_01480"
     CDS             2992..3543
                     /gene="dcd"
                     /locus_tag="VN478_01480"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HWQ21380.1"
                     /translation="MGVRSDIWIAAHARAGMIEPFASDCPKGVVSYGVSSYGYDMRVA
                     DEFKVFTNVFNAIVDPKAFTGRSFVDMKGDSCIIPPNSFALARSLEYFRIPRDVLCLV
                     IGKSTYARAGIIVNVTPLEPEWEGFVTIEISNTTPLPVKVYANEGIAQVLFMGADELC
                     GVSYRDKKGKYQNQTGIWLPRVE"
     gene            3546..>3965
                     /locus_tag="VN478_01485"
     CDS             3546..>3965
                     /locus_tag="VN478_01485"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HWQ21381.1"
                     /translation="MKEIARRLGISTSTVSRAFSKPEMVRPELRERILKMASELNYRP
                     NPIARAMVRGQSDFIGLVVPDITNPFFPAIVRGAEDEARRHGLNLIVCNSDGHVAKEK
                     QYLHSLRDLTVAGVIVSSASRRDAYVSALATDIPVVVM"
BASE COUNT          741 a         1160 c         1246 g          818 t
ORIGIN      
        1 gactatgacg ccaagtacaa ggtgggatac aagaccaccg ccggaacgga gagtggtgtc
       61 cagaaggccg aggcgcgcaa ggtgccggcg aatctgggtg aagacctcac ccatcaggtg
      121 caggaccttg catgccgcgc cttcactgct cttgattgcc ggggcgtcgc ccgcgtggac
      181 ttcctgatcg atgagtcggg gacggtctac gtcaacgaga tcaacacgat cccgggagcc
      241 ctttccttct atctgtggga ggccgtcggc atgcaatacc gggacctgct cacgaagctg
      301 gtcgatctgg cctgcgcggc gttcgaggac cgcaggtcga acatcgtcag ctacgacgtg
      361 tcggatatcc tgaggatggg ttcgaagggc aagtccggaa ccaggaaagc ctgacccggc
      421 ctcatggcga ccacgctcgt cgacggtatc acgcttgcgt atgtagacga agggttcgga
      481 gaccccgttg tcctcctgca cggctggggc ggccaggcgg ccagcatgac acctctcatt
      541 acgggcctga ggcagcagta ccgcatcgtt gcgttcgacc tccctggttt tggcggcagc
      601 agtcctcccc cagttccctg gggaacgccc gaatacgctg cctttgtcga gaaggctgtg
      661 gcgtcgctgg ggatcgcgcg cgcaacctat gtcggacact cgtttggcgg ccgtattgcc
      721 ctgtggttgt catcacacag gcctgagatt gtccaggctc tcgtccttgt cgacgctgcc
      781 ggcatcaagc ctcccacgac ggtcaagcgg cgcatgcggc tcattgttta caagaccgcc
      841 aggatgctcc tgcgtctccc cattctcggg gcaaaaggac ccgcgctcca ggaacgtctt
      901 gccatgcgct tcggaagtgc cgactacaag gcggtctccg gtgtcatgcg gtcgtctttc
      961 gtcaagaccg tgaatcttga tctcagcgac tgtatgcaca cgatcactgc gccgacccta
     1021 ctcatctggg gcgagaaaga ccaggcgaca ccgatcaccg acgggcgtac catggaacgg
     1081 ctgatacagg ggtcgcggct catcgccgtt gccggcgcag ggcacttcag ctacctggac
     1141 agtccgtcat ttgtgaatgc cgtggtgagc gcattccttc acggaacaag ggaagggcag
     1201 gtgtcgtgaa gcccctgacg acttctgtcc tcgcgattgc tgctctcatg tgccttgctc
     1261 tcaccggtgt ccgggccagg tacctcttgc acatgctcca acttgaggaa tacgaccctc
     1321 gccgcttttt ccattgggta gtgggccact cgaagcgcct ctatggcaac tacccgctgg
     1381 atgctcttgg ggtcatgggc ctggctattg tgatcgtcca tccgtccagg cccgttgtcg
     1441 cggcggttag cggtggcatg gcggttgcgc tctgcgtagg tggactgctg caggaattca
     1501 ggtggatgaa ggctcctgcg aagaaggcgc tggtcatgac gccacgagcc tggcgcctgc
     1561 tggcgtgctg cctgatcatc ggcgcggctg cctccgtcgt gcctttcgtc agcagtcggt
     1621 ggtgggatct tgccgcttct ctgctgttgc ccctggcagc gcctctgctc atgctgctca
     1681 gtcgcttcat catggagccc gtcgaggcat cgatccgtga ctggtacgtg cgggacgctc
     1741 gcgggatcct tgccgggttt gctccgacag tcgttggtgt caccggcagc tacggcaaga
     1801 ccagcaccaa gttcttcacg gcgcatctcc tcgagtcgcg atatcgcgtg ctgatgaccc
     1861 ccgagagcta caacacggcc atgggcatct gccgcgtcat ccgcggagag ctcggcccag
     1921 aacacgaggt gttcgtggtg gaattggccg aaaacgagag gggtggattc ggacaccttc
     1981 tccgtctggc tccctgcagc atatcgatcg tgacgtcggt cggcctgcag cacctcgagg
     2041 aattcggttc cgaggctgct atccggcgtg tcatgcaggg atttgtgcgc atgccaggca
     2101 gcggacccac ggtcgtcctg aatggcgacg atcctgtcct gtccgacatc cagggcgtac
     2161 ccgggaagag actgctgagg gcgagcgcaa gtggatcgct gtcatccgat ctgcgtgctg
     2221 agaacgtggc catgggcgct gacggtctgt ccttcgacat cgtgacgagc cagggggagc
     2281 gtttcgccta tcacacgacg gtgctcggcc ggcacaacgt tgagaatatc ctgcttgcgg
     2341 tcgcggtggc gcatgacctg ggagtgtcct ggagcagcat ccaggatggt atctcgacgc
     2401 tgcaggcacc tcctcatcga ctgcagatga tgctcggcgc cggcggcgtg cggatcatcg
     2461 atgatgcatt caactcgaat cctgccggtt ttgccatggc catggaagtg ctggctggtt
     2521 tttcctcacg gcgagtgctt gttacccccg gcctggtgtc cttgggcaca gcggaagacg
     2581 ctgcaaacgt cgaggccggt cgacaggctg ccagagcggc cgacacggtg atcctcgtgg
     2641 gcccagcgaa gactcggcct gttcgagagg gactggtatc ggccggtttc cctgaaacga
     2701 gcattttcac tgctcgttcg ctcgaggagg tcacggctct catgcgaagc ctgctggaac
     2761 ccggggacac cgtgatgttt gagaatgatc ttcccgacac ctacgacgag tgacgtgagg
     2821 ctcgacgaac ggtagtggga gttgaattat cgaggagcga acacgccaat gtccggcact
     2881 gtctgcacgg agaatcgcca tggcggacgc ggacgactgc acttgacggt gtcgtacttc
     2941 ggaacacaat gataacgttt gcaggcgaaa acttggattg ggaggcggga gatgggagtc
     3001 aggtctgaca tctggatcgc ggcccacgca cgggctggca tgatcgaacc ctttgcatca
     3061 gactgcccca agggcgtcgt ctcgtatgga gtctcttcgt atggctacga catgcgcgtc
     3121 gcggacgagt tcaaggtctt caccaatgtc ttcaacgcga tcgtagaccc gaaggcgttc
     3181 actgggcgaa gctttgtaga catgaagggc gactcctgca tcattccgcc aaactccttc
     3241 gctctggcaa ggagtctcga gtacttccgg ataccccgag acgtgctgtg cctcgtcatt
     3301 ggcaagagca catacgcgag agcaggcatc attgtcaatg tcacgccgct ggaaccagaa
     3361 tgggaaggtt tcgtgaccat cgaaatcagc aacacgacgc cgcttcctgt caaggtctac
     3421 gccaacgaag ggatcgccca ggtcctcttc atgggggctg acgagttgtg tggagtctcc
     3481 tatcgggaca agaaagggaa gtaccagaac cagacaggca tctggctccc aagagtggag
     3541 tagcaatgaa ggaaatagcg cggaggctgg gcatttcgac gagcacggtg tctcgagctt
     3601 tctcgaaacc tgagatggtg cggccggagc tgcgtgagcg tatcctgaag atggcgagcg
     3661 agctgaacta caggcctaat ccgattgcgc gtgccatggt ccgtggtcag tccgacttca
     3721 ttgggctagt ggttccggac atcaccaacc ccttcttccc ggccatcgtg cgtggcgccg
     3781 aggatgaggc acgcagacac ggtctgaacc tcatcgtctg caacagtgat gggcacgtag
     3841 ccaaggagaa acagtacctg cactctctgc gtgacctgac ggtggcggga gtcatcgtgt
     3901 cgagtgcctc ccggcgagac gcgtacgtat cggcgctggc gaccgacatt cccgtcgtcg
     3961 tcatg
//
LOCUS       DATMZD010000072         5566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_22190,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000072 DATMZD010000000
VERSION     DATMZD010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5566
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22190"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="VN478_01490"
     CDS             complement(<1..878)
                     /locus_tag="VN478_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HWQ21382.1"
                     /translation="MNGKRIYSIAGGQCGRETLLAILREHPEIRFVSLVAIDLAGNDT
                     DERIPIQTFIADIDAFLAGKAVQTDGSSVALAGMTSLGDAQVDLAPDLTATWFVDYNE
                     QHVDHDTGLPVGTLRIPSFLMHRGERIDSRSLLAKTLDYAQAELLKLVRSHSTSPLLA
                     HCEPGRLAGIEFTIGTELEFWVKTPTDTANIEELSAAQILQENYWQRTRGVARTALEQ
                     AVMALEAYGLHPEMGHKEVGGVKAAIGAGGELTHVMEQMEIDWRYASALQAADNELLA
                     RILVKETFRANGLKVN"
     gene            964..1545
                     /locus_tag="VN478_01495"
     CDS             964..1545
                     /locus_tag="VN478_01495"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019464868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008263 - pyrimidine-specific mismatch
                     base pair DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mismatch-specific DNA-glycosylase"
                     /protein_id="HWQ21383.1"
                     /translation="MRARYERDTCDLTPETGSSAAGELIPDVLADGLDIVFCGSALGD
                     VSWARRAYYANQGNRFWKTLADVGLTPRLLAPQDYPLLQIWRIGLTDLVKTDHGQDAH
                     IFDGHVDLDEARASLRAKVLRWRPRVLAFTSRTAAARFLGDRVSPGRQREGIGRTVVW
                     VLPSTSGLARRFFDIGPWNDVARFVRDLDPGAT"
     gene            1681..2160
                     /locus_tag="VN478_01500"
     CDS             1681..2160
                     /locus_tag="VN478_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21384.1"
                     /translation="MTSDRFSSLHITIPDLRGMLPGVETGVSTAFWVPLAEFVLHRSE
                     TFEVVCQREDTDAIKLLMPVAEMIERRHEDGALMFWGAVTPTVSRAVAGGHGSGHESL
                     WWWQMALTSDGNQVFALQEYGERMDVYGLTADEAAAVLELLPFSARIVRTMEAAGTR"
     gene            2296..3447
                     /locus_tag="VN478_01505"
     CDS             2296..3447
                     /locus_tag="VN478_01505"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HWQ21385.1"
                     /translation="MPGTYYMPGVNLIHRGALNDVGAWAQNLGGTRGLIVASMGSYGE
                     DQGKMVADVLTKSGVASTVYAGAGPNPTDVMVMNGVEIFREQHCDLLVAIGGGSAMDC
                     AKAIGLIVSNGGTIDQYEGEDKVTKQLPPFITVNTTAGTGSEVTSVAVITDSRTHHKM
                     TIVDWRLTADVSVNDAQLMITMPPALTAATGMDALSHAIEAYVATNATALTDSLAYEA
                     ITLVFQWLPRAFEHGEDVAARHGMCNAAFLAGLAFNNSGLGYVHALSHPLTGKYGLPH
                     GVLNAVLLPAVMRWNAVLVGTRMAKIAVAMGVGHPWRTDRENAEKAIVGVQDMNRRLR
                     MPSGLAAMGVKESDIPALTEQAMEELVGRTNPRQVASPEQMSRLYLDSM"
     gene            3486..4253
                     /locus_tag="VN478_01510"
     CDS             3486..4253
                     /locus_tag="VN478_01510"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HWQ21386.1"
                     /translation="MFGAIVGDIVGSAYEYHVVLDEDLPLLTPASSYTDETVLTVAVA
                     DCLLHQRPYGETLRAWALANPGRRYSTRFAKWTEDPERTMPTSYGNGAAGRVSPVGWA
                     FDTLEETLAEARRSAEPTHNHADGIAGAQAVAAAVFLARTGVSKDGIRDYLERTFRYD
                     LRRSLIDCTGGWFEAAELSVPEAVICFLQSTDVRSSLQNAVNLRGDADTQAAIAGSIA
                     EAFYHGIPADLSGPCLAMLPADFRSIIDEFRGRFHIV"
     gene            4271..>5566
                     /locus_tag="VN478_01515"
     CDS             4271..>5566
                     /locus_tag="VN478_01515"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HWQ21387.1"
                     /translation="MLRADGHWFCDEAGRTLLFRGVNLGGDSKVPTTPDGATHHPEGF
                     FDHRMVSFVGRPFPLAEATEHLSRLKSWGLTFLRFLVTWEAIEHAGPGLYDTAYLDYV
                     EQVLRIAGEMGFTTFIDPHQDVWSRFTGGDGAPGWTLEAVGFDMTHFAETCAAIVHQT
                     HGDPFPRMVWPTNAGRLAAATMFTLFFGGNDFAPRVLVDGEPVQEYLQRHYVDAILQV
                     VQRARDVPGVVGYDTFNEPSHGYIGWKDLRGFGSRLRIDLQPTPLQSFALGDGMAQEV
                     EAWGMSIAGGRLRGRRTVNQEGVHAWKEGHPCIWRDQGVWGMADGGEPELLVPDYFCR
                     HEGRDVDFESDYLVPFIRRYAQAVHAVDPKALIFVEGEPESLPPSMGDCPEQGIVSAP
                     HWYDAVQLLLKRPGRWLGYDSIRNRVVLGPVAVRRSFAWQ"
BASE COUNT         1029 a         1619 c         1832 g         1086 t
ORIGIN      
        1 aaattgacct tgaggccgtt ggcacggaac gtctccttca ccaggatgcg ggcaaggagt
       61 tcgttgtcgg ctgcctgaag ggcggaggcg tatcgccagt cgatctccat ctgctccatg
      121 acgtgggtga gttcgccgcc ggcgccgatg gccgccttga cgcctccaac ctccttgtga
      181 cccatctcgg ggtgcaagcc gtaggcttcg agcgccataa cagcttgttc cagggcggtg
      241 cgggcgacgc cgcgggtgcg ctgccagtag ttctcctgca ggatctgtgc ggctgagagc
      301 tcctcgatgt tggctgtatc cgtcggagtc ttgacccaga actccagctc cgtcccgatc
      361 gtgaactcga tgcctgcgag ccgcccaggt tcacagtggg ccagaagggg cgacgtggag
      421 tggctgcgta cgagcttcag gagttccgcc tgggcgtagt ccagggtctt cgccagcagg
      481 gagcgcgagt cgatgcgctc accgcgatgc atcaggaacg acggtatgcg caaggtgccc
      541 acggggagac cggtgtcatg gtccacatgc tgttcgttgt agtcgacgaa ccaggtggct
      601 gtcaggtcag gcgccaggtc gacctgggcg tcaccaagtg acgtcatgcc ggccagtgca
      661 acagatgagc cgtcggtctg gacggccttt ccggccagga atgcgtcgat gtccgcgatg
      721 aaggtctgga tggggatgcg ctcgtcggtg tcattgccgg caaggtcgat cgctacgaga
      781 gagacgaagc ggatctccgg atgttcgcgg aggatagcaa gcagggtctc gcggccacac
      841 tggcctcctg cgatgctgta gatgcgtttg ccgttcatag gaactcctcg atgcagtttc
      901 cctgagacta ccgtgcggag cgttttttgc aagccgcaga cttcacactg ctatactgtg
      961 accatgagag ccagatatga acgggacaca tgcgacctga caccggagac cggcagctcg
     1021 gctgcaggag aactcattcc ggatgtcctg gctgatggcc tcgacattgt cttctgtgga
     1081 tcagcgcttg gagacgtctc atgggcgcgg agagcgtact atgccaatca gggcaaccgg
     1141 ttctggaaga cgctggcaga cgtcgggctg acgccacgcc tgcttgcgcc gcaggattac
     1201 ccgctcctcc agatctggcg aatcggcctg actgatctgg tcaagaccga tcacgggcag
     1261 gacgcacaca tcttcgacgg ccacgtcgat ctggatgagg cacgcgcatc attgagggcg
     1321 aaggtcctgc ggtggcggcc gcgcgtgctc gcgttcacca gcaggaccgc agcggcccgt
     1381 tttctgggtg acagggtctc accgggaagg cagcgggagg gtattgggcg gacggtggtg
     1441 tgggtcctgc cgtcgacgtc cggcctggct cggcgcttct tcgacatcgg accctggaac
     1501 gatgtcgccc ggttcgtccg ggacctggac cctggggcaa cttgattact ggtcgctctg
     1561 ggtacactgt tagtgtctgt tgtcatgtcg cacggggaca gggagaacga tgatttcgga
     1621 gattgttgca gtcgatgtgc acgtcgagcc gactcacgaa cagatgaggc ctgtcacaac
     1681 atgacctccg acaggttctc atcgctgcat atcacaattc ccgatctgag aggaatgctg
     1741 cccggcgtcg agaccggtgt ttcgacggca ttctgggtcc ctctcgccga attcgtcctg
     1801 caccgttcag aaacctttga ggttgtctgc cagcgcgaag acaccgacgc catcaagctg
     1861 ctgatgccgg ttgcagagat gatcgaacgc aggcatgagg acggagccct gatgttctgg
     1921 ggtgctgtca ctcccaccgt cagcagggca gtcgccggag gacacggttc gggccatgag
     1981 tccctgtggt ggtggcagat ggcgctgacg tctgacggca accaggtctt tgccctgcaa
     2041 gaatatgggg agcggatgga tgtgtatggg ctgacggcgg acgaagcggc agctgtactc
     2101 gaacttctcc cgttctcggc acggatcgtt cgtacgatgg aggctgcggg gactcgctga
     2161 gcctccttgc attgtgaccg acctgcggtt gagcagggaa gtggtttgcg gtatagtgat
     2221 gacctgatgt gctgtacctg cacacatggc gcacgggcct gcgagcctgg ctcgacgtga
     2281 cttggaggtg gcaatatgcc cggaacgtac tacatgccag gggtcaatct catccatcgt
     2341 ggggcgctga acgatgtcgg tgcctgggcg cagaacctcg gcggaaccag gggtctgatt
     2401 gtcgcatcca tgggatcata cggcgaggat cagggcaaga tggttgcgga cgtgctgacg
     2461 aagagcggcg tcgcgtctac cgtctacgcc ggtgcagggc cgaatccgac cgacgtcatg
     2521 gtcatgaatg gcgtcgagat cttccgggag cagcactgtg acctcctcgt agctatcggt
     2581 ggtggcagcg ccatggactg tgcaaaggcc atcgggctca tcgtcagcaa cggcggcacg
     2641 attgaccaat atgaagggga ggacaaggtg acgaaacaac tgccaccctt catcaccgtg
     2701 aacacgacgg ccgggaccgg cagtgaagtg acatcggtcg cagtgatcac ggacagcagg
     2761 acacaccaca agatgacgat cgtcgactgg agactgacgg cggacgtgtc cgtcaatgac
     2821 gcccaactga tgatcaccat gccgcctgct ctcactgccg ctacaggcat ggacgcgctg
     2881 tcgcacgcca ttgaagccta tgtcgccacg aatgccacgg cgctcaccga ttcgctggct
     2941 tacgaagcca tcacgctggt attccagtgg cttccacggg cgttcgagca cggcgaggac
     3001 gtcgcggccc ggcacggcat gtgcaatgcc gcatttctgg ctggtctggc attcaacaac
     3061 tcgggtctcg gatacgttca cgcgctgtcg catccgctga ccggcaagta tggtctgccc
     3121 cacggcgttc tgaacgccgt cctgctcccc gcagtcatgc gatggaatgc agtactggta
     3181 gggacgagaa tggcgaagat cgcggtggcc atgggcgtcg gtcatccgtg gcgcacggac
     3241 cgggagaacg ccgagaaggc cattgtcggt gtccaggaca tgaaccgccg tctcaggatg
     3301 ccgtcaggac ttgctgcgat gggcgtcaag gagagcgata tcccagcgtt gaccgagcag
     3361 gccatggagg aactggtagg caggaccaat cctcggcagg ttgcatcgcc tgaacagatg
     3421 agccgcctgt acctggactc gatgtagcac ggaccgtctc gcgagaccag ggacctcttg
     3481 gcgtcatgtt cggcgcgatc gtcggcgaca tcgtcggctc tgcatacgag taccatgttg
     3541 ttctggatga ggacctgccg ctgctgaccc cggcatcctc ctacaccgac gagacggtac
     3601 tgacagtcgc tgtcgcggac tgcctgctgc accagcgtcc atacggcgag acactgcgtg
     3661 cgtgggcgct tgctaacccc ggccgcaggt acagcacacg tttcgcgaag tggaccgagg
     3721 acccggaacg gacgatgccc acgagctatg gcaatggagc tgcaggccgt gtcagtcctg
     3781 tcgggtgggc gtttgacaca ctcgaggaga cactcgcgga ggcgcgtcgc agtgctgagc
     3841 ccacgcacaa tcacgccgac gggatcgcgg gggcgcaggc cgtcgcagcg gctgtcttcc
     3901 ttgcgaggac gggagtgtcg aaggacggta tccgggacta tctggaacgc acgttcagat
     3961 atgatcttcg gcgttcgttg atagattgca cgggaggatg gttcgaagct gcagagctgt
     4021 cggtcccgga ggctgtcatc tgcttcctgc agtccactga tgtccgcagc agcctccaga
     4081 acgccgtgaa tctgagggga gacgcagaca cgcaggcagc catcgcgggg agtattgccg
     4141 aggcgttcta ccatggcatt ccagctgacc tgagtgggcc gtgcctggca atgctcccgg
     4201 ccgacttccg gtccatcatc gatgagttcc gtggacggtt tcatatcgtc tgattgccgg
     4261 aaggagactg atgctgcgcg ctgacggaca ctggttttgc gatgaagcag gacgcacgct
     4321 cctgttccgc ggcgtcaacc tggggggcga cagcaaggtg ccaaccactc ccgatggtgc
     4381 aactcatcac ccagaggggt tcttcgacca ccgcatggtc tcatttgtgg gtcggccgtt
     4441 tccgctcgcg gaggcgacgg agcacctgag caggctgaag tcgtggggtc tgaccttcct
     4501 gcggttcctg gtgacgtggg aggctatcga acacgcgggc cccggactgt atgacaccgc
     4561 gtacctcgac tacgtcgaac aggtcctgcg gatcgccggc gaaatgggct tcacgacgtt
     4621 catcgatccg caccaggacg tatggagccg gttcaccggg ggcgatgggg cacccggctg
     4681 gaccctcgag gcggttggtt tcgacatgac gcatttcgcc gagacctgtg cggccatcgt
     4741 ccaccagact catggcgatc cgtttccccg gatggtctgg cccacaaacg ccggtcggct
     4801 cgccgctgct acgatgttca ccctgttctt cgggggaaac gactttgctc ctcgcgtcct
     4861 cgtcgacggt gaacccgtcc aggagtatct gcagcgtcac tacgtggatg ccattctcca
     4921 ggtagtccag cgtgccaggg acgtgccggg tgtcgtgggg tacgacacgt tcaatgagcc
     4981 gtcacacggc tacatcggat ggaaggacct cagggggttt ggcagccgtc tcaggatcga
     5041 cctgcagcca acgcctctcc agtcgtttgc cctgggcgac ggaatggcgc aggaggtgga
     5101 ggcctgggga atgagcattg ccggagggcg tcttcgcggg cgccggaccg tcaaccagga
     5161 aggagtccac gcctggaagg aaggtcatcc ctgcatctgg cgtgaccagg gagtgtgggg
     5221 aatggccgat ggcggtgagc cggaactcct cgttcctgac tacttctgtc ggcacgaggg
     5281 acgcgacgta gacttcgaga gtgactacct tgtgcctttc atacgcagat acgcacaggc
     5341 tgtccacgcc gtggacccca aggctctgat tttcgtcgaa ggtgaacccg agagtctgcc
     5401 accgtcgatg ggcgactgcc ccgagcaggg catcgtgtct gcgccacact ggtacgacgc
     5461 tgtgcagctc ctcctcaagc gaccgggcag gtggctcggc tatgacagca ttcgtaaccg
     5521 ggtcgtgctg ggccccgtcg ctgtccgtcg aagcttcgcc tggcaa
//
LOCUS       DATMZD010000073         2719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_42341,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000073 DATMZD010000000
VERSION     DATMZD010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42341"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="VN478_01520"
     CDS             <1..665
                     /locus_tag="VN478_01520"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HWQ21388.1"
                     /translation="SDLKATHWASGFITLMTQEGFMTGDPDGRFRADDPMTREEAAAA
                     FLRIAGMSPVLNPSSSSFKDVQPMSWSAGYIEAARTAGLLAGYPDNTFRPADLLSRAE
                     FATLASNALGRVLTTTNTRVNPQNEVKWPDVPADFWAYNYILEVSTPHTVTSPTRLTR
                     SITLKDRKIPLFSEGENGIITFLHLGDTITAIVPVDGLQADGSDPAPRQVKVRILNHE
                     RP"
     gene            complement(832..>2719)
                     /locus_tag="VN478_01525"
     CDS             complement(832..>2719)
                     /locus_tag="VN478_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21389.1"
                     /translation="LTAVLITVFSLGRGVPALSANDLDLVNQTTVLEVTEGSLQYVGD
                     TASGSVFLSDRGLELSAKDGTTRVLVTAAVLQDGATVGPASVALSPSGGQAVIDVEGT
                     IVLVDLATGSRRVLVDSTESTFYASPVFVSDHEIAAGRKTTSIQSGKLVIDNFAGIVT
                     IDVANGAVHVLAEQGAAPSVTSDGTKLVYSESGTDHPLVVVDLRTGTSTHYGQEGDHT
                     VVVSPNGRYAVFMTGKEDLVVTDADSFTVRRILSRGDFYDLRWSSDSTSFYLLKFASI
                     EGWSATYLKRFVLGRTDVHDARDVVVKYLNALILHQDDLMKSLWTSISISGPLSGSTG
                     PQPIGYSIDGVREAGGTTLVDATITYEYAEQPRTDVVFRTFSVVQRDGRYLVSAIDER
                     YLKSAIYDRGSIPFLQFLGGDGSIFLSIVDDEPSTAIFTWTGAFDEQVFQETGKHITG
                     WGVSGKWGDDANRQTGATPLDPTGWHLTTLHYDNDAGWLVFALQSVDPVAYRLYAAKV
                     NDRQLPVTMAQFIGGADAYAETVVMNEDGRALVSSRHLPGDPLLSSVIPSFEVYDLGT
                     RAEAAVDRSALDAFCTRGGWSLSLSFWQGQDIGVQLVRRLSDATVTSQIVTVDGTTGA
                     VSED"
BASE COUNT          538 a          895 c          798 g          488 t
ORIGIN      
        1 tctccgacct gaaggcaacc cattgggcat ccgggttcat cacgctcatg acacaggaag
       61 gcttcatgac gggcgacccc gatggaaggt tcagggcgga cgaccccatg acacgcgaag
      121 aagctgccgc cgcgttcctg cgcatcgccg gcatgtcgcc ggtcctcaac cccagctcgt
      181 cctccttcaa agacgtgcag ccgatgtcct ggtcagcagg gtacatcgag gcggcacgta
      241 ctgccggact cctcgcgggc tatcccgaca acacgttcag gcccgctgac ctcctgtccc
      301 gcgccgagtt tgcaacactc gcgagcaacg cgctcggccg ggtgctgacc acgacgaaca
      361 cacgggtgaa cccacagaac gaagtgaagt ggccggacgt gcccgctgac ttctgggcat
      421 acaactacat ccttgaagtg tcgacgcccc acacggtcac gagcccgaca cgcctgacac
      481 gcagcatcac actgaaggac cgcaagatcc cgctcttctc ggaaggggag aacggcatca
      541 tcaccttcct gcacctgggc gacacgatca ccgccatcgt cccggtggac ggtctccagg
      601 cggacggctc cgatcctgct ccgcgccagg tgaaggtccg catcctcaat cacgagcggc
      661 cctaagggat aagcacaaga tcacatcggc cggtctgccc ttctgggcag accggctttt
      721 ctactgtcga cggctatacc tctgtcacca gcctttgtct gtcggttatc atgacaaatg
      781 tgcgccccca ctcaggcaag cgcacatttg ctctcgctac aagaagggag actagtcttc
      841 gctaaccgcc ccggtcgtgc cgtcgaccgt gacgatctgt gacgtgacgg tggcgtcgga
      901 aagacgccgg accagctgaa ctccgatgtc ctgcccctgc cagaatgaga gggacagtga
      961 ccagccgccc ctggtgcaga atgcatccag cgccgaccgg tcaaccgctg cctcggccct
     1021 ggttccaagg tcatagacct cgaacgacgg gatgaccgag ctgaggagtg gatccccggg
     1081 aagatggcgg gacgagacca gcgcccgccc atcctcgttc atcaccaccg tctcggcata
     1141 ggcgtccgcc ccaccgatga actgcgccat ggtcacagga agctgccggt cgttcacttt
     1201 ggccgcgtac agtcggtaag cgaccggatc gacgctctgc agggcaaaca cgagccagcc
     1261 tgcatcattg tcgtagtgca aggtcgtgag gtgccatccc gtggggtcca gcggtgtggc
     1321 gccggtctgc cggttcgcgt catcgcccca cttgccggaa acgccccacc cggtgatgtg
     1381 tttgcccgtc tcctggaaga cctgctcatc aaacgccccg gtccaggtga agatcgccgt
     1441 cgatggttcg tcgtccacga tgctgaggaa gatggaacca tccccaccca ggaattgaag
     1501 gaacggtatg gacccccggt cataaatggc gctcttcagg taccgctcgt cgatcgcgct
     1561 gaccaggtag cggccgtctc gctggacgac ggagaacgtc cggaagacga catcggtgcg
     1621 cggctgctcg gcatattcgt aggtgatcgt cgcatccaca agcgttgtgc cgcccgcttc
     1681 gcgcacgccg tcgatgctgt atccgatcgg ctgcgggccg gtcgatccgc tcagcggacc
     1741 gctgatcgag atggacgtcc acagcgattt catgaggtcg tcctgatgga ggatcaaggc
     1801 gttcaggtat ttcacgacga cgtcgcgcgc gtcatgtaca tcggtacgcc cgagcacgaa
     1861 ccgcttgaga taggtggccg accatccctc gatgcttgcg aatttcagga gatagaagct
     1921 cgtgctgtcc gagctccatc ggagatcata gaagtcccct ctgctcagga tacggcgaac
     1981 ggtgaatgaa tcggcatccg tgacgaccag gtcctccttg ccggtcatga aaacggcata
     2041 ccggccgttc ggcgagacca ctaccgtgtg atcgccttcc tgtccgtaat gggtgctcgt
     2101 cccggtcctg aggtccacca caacgagagg gtgatcggtg ccggactcgg aatagacgag
     2161 cttggtgccg tccgacgtca cagagggggc cgccccctgc tcggcaagaa cgtgcaccgc
     2221 accgtttgcc acgtcgatcg tcacaatgcc cgcgaagttg tcgatgacca gctttccact
     2281 ctggatcgac gtcgtcttgc ggccagccgc gatctcatgg tcacttacaa agacagggct
     2341 ggcgtaaaag gtcgactccg tgctgtccac aaggactctg cgggagccgg tggcaaggtc
     2401 caccagaacg atagtgcctt cgacgtcgat gacggcctgt ccgccgcttg gcgaaagcgc
     2461 caccgaagcc ggaccgacgg tcgctccgtc ctggaggaca gccgcggtga ccagaacgcg
     2521 cgtcgttcca tccttcgcag agagctccag tcctctgtca ctcaagaaca cgctgcccga
     2581 cgccgtgtcg ccgacatatt gcagactacc ctcggtgacc tccaggaccg tcgtctggtt
     2641 caccaggtcc aggtcgtttg ccgacagcgc cggcaccccg cgaccaagcg agaacaccgt
     2701 gatgaggact gccgtaagc
//
LOCUS       DATMZD010000074         4497 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_32475,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000074 DATMZD010000000
VERSION     DATMZD010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4497
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32475"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VN478_01530"
     CDS             complement(<1..312)
                     /locus_tag="VN478_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008519672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HWQ21390.1"
                     /translation="MSVMVDLNSDVGESFGVWTLGDDERVLDRVSSANVACGFHAGDP
                     QVMLTTVRAARDRGVAVGAHPGYPDLVGFGRRAMTCTPDEVYASCLYQIGALAALCRA
                     EG"
     gene            complement(296..2101)
                     /gene="pxpB"
                     /locus_tag="VN478_01535"
     CDS             complement(296..2101)
                     /gene="pxpB"
                     /locus_tag="VN478_01535"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HWQ21391.1"
                     /translation="MKCAEWPMTVVQCGDCSRLVVVGHNISPLTNARVHALRRAIEAL
                     HPVWLVETVPGYCTLGLVVRPLLVSQGDVEELVSVAARSVMAAGPLQSATVTIPVCYG
                     GAYGPDMEAVCRRSELSEAEVVRRHTASGYECSMLGFLPGFPYLMGLDPQLATPRLAV
                     PRVAVPAGSVGIAGGQTGVYPVSSPGGWNIIGRTPLTLFDPSRKQPSLVQAGDTVCFT
                     SISPLEFEHQRLHEFTHYPQICDVCEPDHSQCEVLEPGMLTTVQDAGRRGLQHMGVPV
                     SGVMDRQALALGNLLVGNDPETAALEITLSGPCLAFRADALVALVGADVDLQVDGNDI
                     PAWTAVLIRAGSVLTIGSPTGAGCRAWLCIAGGIDVPNLLGSRSTLLRGSLGGFEGRA
                     LRAGDVLRLFPLTHDARRLEGFSCPAGLRESDSEDMFVPVLPGPQIESLKPDARQVFL
                     ETTWAVSAVSDRMGCRLEGPPLDLNGGADVISEAVPEGAVEVASSGLPIIMLSDCQTI
                     GGYVKPFVVASAALGRLAQRRPDDSVRFRVCSLDEACTMLEKQLSARERLAGLQAEWR
                     QRRLGGTLHMTVNGLSHAVEWQDVTPLEVDDERDG"
     gene            complement(2252..2833)
                     /locus_tag="VN478_01540"
     CDS             complement(2252..2833)
                     /locus_tag="VN478_01540"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HWQ21392.1"
                     /translation="MVHVLPELPWAKDALAPIISAETIEYHYGKHHQAYVTNLNKLIE
                     HTTMEEEPLEKIILTAPMGGIYNNAAQVWNHSFYWKCMKPNGGGEPAGALADLLIKGF
                     GSVQAFREKFTAAAVGTFGSGWAWLVRNRDGTLTVESTGNAMNPLRDGKQPLLTCDVW
                     EHAYYIDYRNARAKYVEAWWNLVDWAFAASNLG"
     gene            3115..3822
                     /locus_tag="VN478_01545"
     CDS             3115..3822
                     /locus_tag="VN478_01545"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HWQ21393.1"
                     /translation="MLPRVVIHNAVSADGRIDWFEADLGAFYDLAGRWHEDCALVGSG
                     TILAAPESAKADPEPWEPHIVDASDTRPLLAIVDSRGRVRCWKTWLDMPYWRGGVALC
                     SETTPDEYLQYLAKAGVDFIVAGSGKVDMRAALEELNARYGIETVRVDSGGILNGILL
                     RAGLVDEVSILVHPTLVGGTTQRSMFRAPNLTSPAGVIALTFVSAQTVGEGLVWLRYK
                     VAGRKRKAPRKTQDRPS"
     gene            3962..>4497
                     /locus_tag="VN478_01550"
     CDS             3962..>4497
                     /locus_tag="VN478_01550"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HWQ21394.1"
                     /translation="MITRIPAQERHLVELSWMQAYWLFSYGEWYVPGNVRLGKLRVFN
                     DGLIQPREGFGMHPHEELEIVTIMLEGQLTITDDTGERTVIHTGEVHRISAGTGTRHM
                     EKNQAADLARYYQIWIFPDEPGLIPSHEQKDFSRMSRQDQLLTVASGRGDPGAVTLHA
                     DAAVSLSTFSPAFALEYS"
BASE COUNT          867 a         1444 c         1377 g          809 t
ORIGIN      
        1 tccctcggca cggcacagag cagccagagc cccgatctgg tacagacaag acgcatagac
       61 ctcatccggg gtacacgtca tggcccgccg cccgaatccg acgagatccg gatatcctgg
      121 gtgggctccg accgctacac cacggtcacg cgcggctctc accgtcgtca gcatgacctg
      181 cgggtcgcct gcgtggaagc cacaggccac attggcgctc gagacgcggt ccaggacacg
      241 ttcgtcgtca ccgagagtcc atacgccgaa gctctcgcct acgtcgctgt tgaggtcaac
      301 catcacgctc atcatccacc tccaacggag tcacgtcctg ccactcaacc gcatgcgaca
      361 ggccattcac ggtcatgtgc agtgtaccac ccaggcggcg ctgacgccac tcggcctgca
      421 gtccggccag cctctctctt gcagataact gcttctccag catcgtgcat gcctcatcga
      481 gagagcacac gcgaaagcgg acactgtcgt ccgggcgacg ctgtgccagc cgtccgagtg
      541 ctgccgaagc gacgacgaac ggcttgacgt atcctccgat cgtctggcag tcggacagca
      601 tgatgattgg gaggcccgaa ctcgccacct cgacagctcc ctcagggaca gcctcggaga
      661 tgacgtctgc accaccgttc aggtccagcg gaggcccctc cagccgacac cccatgcggt
      721 cactcacagc gctcacagcc catgttgtct ccaggaacac ctgccgtgcg tcgggcttca
      781 gtgattcaat ctgcggaccg ggcaagacgg ggacgaacat gtcctcggag tcggactctc
      841 gcaagccggc gggacaggag aagccctcga ggcgccttgc atcatgcgtc agagggaaga
      901 gccgcaggac gtctccggca cgcagtgcac gtccctcaaa cccgcccagt gagccccgca
      961 agagtgtcga acggctgccc agcaggttgg ggacatcaat gccgccggca atacacagcc
     1021 aggcgcggca tcccgctccc gttggactgc caatagtgag gacgcttcca gctcgtatca
     1081 ggactgccgt ccacgcaggg atatcgttgc catccacctg caggtccacg tcggcgccga
     1141 ccagggccac gagtgcgtcc gccctgaagg ccagacatgg gccggacagc gtaatctcca
     1201 gagctgcggt ttctggatcg ttgcccacca gaaggttccc gagtgccagc gcctgacggt
     1261 ccatcacgcc cgaaacgggg actcccatgt gctgcagtcc gcgtcgtcca gcgtcctgga
     1321 cggtcgtcag catacccggc tcgagtacct cgcactggga atggtctggt tcacaaacat
     1381 cacatatctg tggataatgt gtgaactcat ggagtcgctg gtgctcgaat tccagggggc
     1441 tgatggaggt gaaacaaacc gtatcccctg cctgcaccag cgacggctgt ttgcgcgacg
     1501 ggtcgaacag ggtgagagga gtgcggccga tgatgttcca gcctcccggg ctggagaccg
     1561 ggtagacgcc ggtctgcccg ccagctatgc cgacgctgcc cgctggaacg gcgactcttg
     1621 gcaccgcgag gcggggcgtc gccaactgcg gatcgaggcc catgaggtag gggaagcctg
     1681 gaaggaagcc cagcatggag cactcatatc cacttgccgt atgtcgtcgc acgacctccg
     1741 cttcggacag ttcggaacgg cggcatactg cctccatgtc gggaccgtat gcgccgccgt
     1801 agcagaccgg gatcgtgacc gtcgcggatt ggaggggtcc agcagccatc acgctccgtg
     1861 cggccacaga gacaagctcc tcgacatctc cctgtgagac cagcaaagga cggacgacca
     1921 gtcccagggt gcagtatccc gggaccgttt ctaccagcca gacaggatgc agcgcttcga
     1981 tggcacgtcg gagcgcatgg acgcgcgcat tggtcaaggg cgagatgtta tggccgacca
     2041 cgaccagccg gctgcagtcg ccgcattgca ccacagtcat cggccattca gcgcatttca
     2101 tggcatcatg atagcccggt tcgcccatca tgtctgtccc gcgcagacgg gaagccacac
     2161 ggaaacggga ggctgctgaa cagcagcctc ccgcctgaag cgcaacggcc gtcaagcgca
     2221 accggtgtca aggctgcgct gccatcgaat ctcagccgag gttgcttgca gcgaacgccc
     2281 agtcgaccag gttccaccac gcttcgacgt acttggcgcg cgcattgcgg tagtcgatgt
     2341 agtatgcgtg ttcccagaca tcacaggtca gcaggggctg cttcccgtcg cgtagcgggt
     2401 tcatggcgtt gcccgtgctc tcgacggtga gcgtgccgtc acggttgcga accagccacg
     2461 cccacccgga gccgaatgtc ccgactgctg cagccgtgaa cttctcccga aacgcctgga
     2521 cggagccaaa tcccttgatg agcaggtctg ccagggcgcc tgcaggttct ccaccgccat
     2581 tcggcttcat gcatttccag tagaacgaat gattccatac ctgtgccgcg ttgttgtaga
     2641 tgccacccat tggcgctgtc aggatgatct tttccagtgg ctcttcctcc atcgtggtat
     2701 gctcgatcag cttgttcagg ttggtgacat aggcctgatg gtgcttgcca tagtggtact
     2761 cgatcgtttc cgcagagatg atgggggcca acgcatcctt ggcccacggc agttccggca
     2821 gtacatgtac cattgaacac ctcctgtggg ttttgcgcta catcgtgtca gagcatcctg
     2881 acggtttcca gtgtatcgcg agcgctcgac aatacgagac cctgctgagc tgcttctgcg
     2941 aagccgccgg caaacgatct gccattccca cagacgagca agtccattca ctacgaggca
     3001 gccatcgtcc ccgggcccgg agaacgctgt ggacacatcc actcccccct gcggcgcaac
     3061 gttgaccccc gctgctcccg cggtatgatg cagtcaatcg acagggggtg tcacatgttg
     3121 ccacgagtag tcatacacaa tgcagtgagc gccgacggac gtatcgactg gttcgaagcg
     3181 gacctggggg ccttctatga cttggctgga cggtggcatg aggactgcgc gctggtcggc
     3241 tccgggacga tcctggcggc gccggaatcg gcgaaggcgg acccggagcc ctgggaaccg
     3301 cacattgtcg acgcgtcgga cacccgtcca ctgttggcga tcgtggacag ccgaggacgg
     3361 gtccgctgct ggaagacctg gctggacatg ccttactggc ggggcggcgt tgcactctgc
     3421 tcagagacca cgccggacga gtacctgcag tatcttgcca aggcaggcgt cgacttcatc
     3481 gtcgccggat ccggcaaggt cgacatgagg gccgcattgg aggagctgaa cgcgcgctat
     3541 ggcatcgaga ctgtgcgcgt ggactcgggt ggtatcctga acggcatact gctgcgcgcc
     3601 ggactcgtcg acgaggtcag catcctcgtg catcccaccc tggtgggggg gacgacccag
     3661 cgttcgatgt tccgtgcccc gaacctcacg tctcccgccg gcgtcatcgc tctcacattc
     3721 gtcagcgctc agacggtcgg cgagggcctg gtgtggctgc gctacaaggt cgccgggagg
     3781 aagaggaagg cgccccgcaa gacgcaagac aggccgtcat gagactgccc gcgcaccatc
     3841 ctgctgcttg actcagggca ggttcgctct atacctgagg tagatgcagg cagaggagaa
     3901 tcccgccggt ccgcatgctt cgcgttgcag gttctgtacg gtcgtcacat gggggtgctt
     3961 catgatcacc cggataccgg cacaggaacg ccatcttgtc gaactgtcat ggatgcaggc
     4021 ctattggctg ttctcctacg gcgagtggta cgtcccgggc aacgtgcgcc tgggcaagct
     4081 gcgtgtgttc aacgacggac tcatccagcc aagggaaggc ttcgggatgc atccccatga
     4141 ggagctcgag atcgtcacga tcatgctgga gggccagctg accatcacag atgacacggg
     4201 cgagcgcacg gtcattcaca ccggcgaggt gcaccggata tcggccggaa cggggacgcg
     4261 gcacatggag aagaaccagg ctgcggacct ggcccggtac tatcagatct ggatcttccc
     4321 cgatgagcct ggactgatac cgtcgcacga gcagaaggac ttctcacgga tgtcgcggca
     4381 agaccagctg ctcacagtgg catcgggaag aggagatccg ggcgccgtga cgttgcacgc
     4441 cgacgctgcc gtctcgctat cgaccttctc cccggccttt gccctcgagt actccat
//
LOCUS       DATMZD010000075         8059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_62484,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000075 DATMZD010000000
VERSION     DATMZD010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8059
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62484"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="VN478_01555"
     CDS             <1..726
                     /locus_tag="VN478_01555"
                     /inference="COORDINATES: protein motif:HMM:NF013397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HWQ21395.1"
                     /translation="YIAFGAEAYLLAISDSASFLGFGLSRILGGIVFSVGLIFVVLAG
                     GELFTGNSLVALSACCRRSSWRGVLRNWALVYLFNIAGSLVVAFLIFGARQYTMGDGA
                     VGVAALKVAAAKVSMPFWTAFFRGVLCNWLVCLAVWMAGGATDTAGKVLAIVPPVMVF
                     VASGFEHSVANMFLVPLGLLLKRVPSVVAAAGTVAGIQQLTWVRGFLVGNLLPVTLGN
                     LAGGVLFVAVPYWIVYARSTESR"
     gene            723..1349
                     /locus_tag="VN478_01560"
     CDS             723..1349
                     /locus_tag="VN478_01560"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HWQ21396.1"
                     /translation="MNLVLLAGGRSQRMGRPKCNMELSGRLLFEYPVLALDGLCSRCI
                     VVANDGSIDPGDVSCILVHDLIAGAGPLGAIYTGLAASDSWLNLVVAGDMPCASPQLA
                     AAMETYALRNGLDIVYPNIDGLLEPLFAIYSKRVVTVARALLDAGRRSVRELYASASL
                     RVGVVDRQFVTRYDRKLTSFFNVNTPDDLIMARLMMHVREKSLQEECV"
     gene            1352..1846
                     /locus_tag="VN478_01565"
     CDS             1352..1846
                     /locus_tag="VN478_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HWQ21397.1"
                     /translation="MNLEDRLKENPELKKQFDLLDAYIGELATPDGSLINVLHRAQEI
                     FGYLPDEVLAFVSGRLNVPRSTVYGVVTFYHFFSRVPKGRHTVSVCLGTACYVRGGNA
                     VYEKVMKTLGIKAGETTKDGRFSLDLVRCVGACGLAPVVVIDKDTYGRSTPEKVATLL
                     NKYV"
     gene            1870..2238
                     /locus_tag="VN478_01570"
     CDS             1870..2238
                     /locus_tag="VN478_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HWQ21398.1"
                     /translation="MKSLEELKRIKEQTLKEMDLRQAKERGKVVVAMGTCGIAAGAKD
                     ALVAIIDELEKADIHDVKVVQSGCMGLCDKEPTVEVSMKDQPAVIYGDVNDANARRIV
                     REHVQGGNVVADLVIKRENI"
     gene            2263..4047
                     /locus_tag="VN478_01575"
     CDS             2263..4047
                     /locus_tag="VN478_01575"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014451659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HWQ21399.1"
                     /translation="MIRNQLLVCAGGACITSGEKSVKDVLVDELKKSGIEDEVQVIET
                     GCMGACDLGPIVVVYPEGTFYQKVTVKNAALIVNEHLVKGRTVGELLYTEPTSEKPAQ
                     TSGDIPFYTRQVKRVLRNCGAIDPLSIEEYIARDGYFALGKALEMTQDDIVQLLIDSG
                     LRGRGGAGFPTGLKWKATRAAQGSPKYVVCNGDEGDPGAFMDRSVLEGDPHSIIEAMA
                     IAGYTVGAEKGYMYVRAEYPLAIERFEYAIGKAREYGFLGKGILGTEFNFDIEIRIGA
                     GAFVCGEETALLRSIEGKRGTPSVKPPFPAQSGLWGKPTLINNVETYANIPQIITNGA
                     EWFRSVGTVKSPGTKVFALAGNVQNTGLVEVPMGTTLRELVFDIGGGIPGGKKFKAAQ
                     TGGPSGGVITPDYLDMPIDYDTLKEIGTIMGSGGLIVLDETSCMVDVAKFYLGFTVDE
                     SCGKCTPCREGTRQMYHMMEQICDGKGRDGDIERLTKLGKGIIDTSLCGLGQSAPNPV
                     LSTIRFYEDEYRAHIYEKKCPSHVCKHLLTYSITPEKCVWCTACARQCPVQAITGEVK
                     KTHVIDQSKCIKCGSCYETCRFGAIRKE"
     gene            4078..5844
                     /locus_tag="VN478_01580"
     CDS             4078..5844
                     /locus_tag="VN478_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012813579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HWQ21400.1"
                     /translation="MLNVKINGRDYQVPDGTTILKAARDYAKIDVPTLCYLEGISEEG
                     ACAICVVEVKGARNLQRACVVKVSEGMEVFTNTARVREARKLNVELLLANHPKDCFTC
                     EKNQICTLRELAAELGVREVQFPRTRTEWLPFDNTSLSLVRDPNKCVLCRRCVNVCAK
                     VQSVYAIDIVGRGSNSSVSTFLDEGLGNVECVNCGQCLLVCPTGAITERSEIPEVWKA
                     LGNPDKVVVVETAPAVRVAIGEEFGMPDGTQATGKMAAALRGLGFDKVFDTDFSADLT
                     IMEEGFELIGRIKNGGTLPLITSCSPGWVKFVETFFPKVLDHVSTCKSPQQMFGTVTK
                     TYWAEKMGIDPRKIVVVSIMPCTAKKFEKERPEMKTAFHYWQEKMGLKDEEVFNDVDC
                     VLTTREAAQMIREAGLDFANLPDEDFDKPLGESTGAAVIFGATGGVMEAALRTAYEVL
                     TKKTLAKLDFEDVRGLEGIKTADVDIDGLILKVAVANGLGNARVLMEQIQKGESPYHF
                     IEIMCCPGGCLGGGGQPISQNPDIKQRRAEAIYREDAGKPIRKSHENPEITQIYKDFL
                     GEPNSEKAHHLLHTEYCDRSCK"
     gene            5921..6184
                     /locus_tag="VN478_01585"
     CDS             5921..6184
                     /locus_tag="VN478_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0065007 - biological regulation [Evidence
                     IEA];
                     GO_process: GO:0065009 - regulation of molecular function
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TM1266 family iron-only hydrogenase system
                     putative regulator"
                     /protein_id="HWQ21401.1"
                     /translation="MDSTKHVGVIAIIISKRETTAPKVNQILTRHGDLIIGRLGLPYG
                     DHGLHVISLIVDGEKEQLQSLTAGLTAVPDTIVESTMSPVCFP"
     gene            6440..6973
                     /locus_tag="VN478_01590"
     CDS             6440..6973
                     /locus_tag="VN478_01590"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HWQ21402.1"
                     /translation="MELDNSTRPAGQTQPLSDEDKLFLRELVETNRIEHHGRLSYMDI
                     MFDLEHRYGNSLPGSVLGEVARLVETSPAQLNGFVTFYTMLSTTPRGKHVIRVCTSGP
                     CHVTGSPAILNSLRQLLGVEIGETTPDGEFTLEGSSCLGICGVSPALTIDDDAYGNLT
                     PEDLSRILDAKRAGGAQ"
     gene            6970..>8059
                     /locus_tag="VN478_01595"
     CDS             6970..>8059
                     /locus_tag="VN478_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013932834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP oxidoreductase"
                     /protein_id="HWQ21403.1"
                     /translation="MKLFSTQIVVPVDSNSVLQGALEVKHVLEETIAKYNLGGQVSVV
                     ETGALGIYDQGVVLAVFPDDVYYGGVKPADIEEIVTEHLLKGRIVSRLSIDRNLSSRR
                     LVEKETGKSQQKIVLRNAGLIDPQSIDEYIARDGYGALAKALAMKPEEVVQVLTDSKL
                     QGRGGAGFPTGLKWGMVAKEQSDEKYIVCNADEGEPGTFKDRLILEGDPHSIVEAMTI
                     AGYATGAHVGIAYIRGEYTMSIARLKKAVKDATEYGLLGKDILGTGFQFDIEVYRGGG
                     AYVCGEEFALLESIENKRGTPRNKPPFPPQVGLFGKPTVVNNVETFANVPPILLNGSD
                     WFKSFGTPTSAGTKVFSLSGDIVNRGIVE"
BASE COUNT         1749 a         2140 c         2490 g         1680 t
ORIGIN      
        1 tatatagcct ttggcgcgga ggcctatctc cttgcgatct cggatagcgc atccttcctt
       61 ggttttggac tgtcaaggat cctcggcggc atcgtcttca gtgttggcct gatcttcgtg
      121 gtgctcgcag gcggcgagct gttcactggg aacagtcttg tcgccttgtc cgcctgctgt
      181 cgccgttcgt catggcgtgg ggtcctgcgc aattgggctc tggtctatct cttcaacatc
      241 gccgggtcgc tcgttgtggc gttcctgatc tttggtgcaa ggcaatacac gatgggagat
      301 ggtgccgtcg gcgttgctgc gttgaaggta gccgccgcga aagtgagcat gccgttctgg
      361 accgcattct tcaggggggt tctgtgcaac tggctcgtgt gtctcgcagt ctggatggcc
      421 ggcggtgcta cggacacagc gggcaaggta cttgcgattg tccctccagt gatggtattc
      481 gtcgcctctg gattcgagca tagcgttgcc aacatgttcc ttgtccctct gggcctcctg
      541 ttgaagaggg ttccgtctgt cgtagccgcc gcaggaactg tggctgggat ccagcagctt
      601 acctgggtgc gcggatttct cgttggcaac cttcttccgg tgacactggg caatcttgcc
      661 ggcggagtcc tgtttgttgc cgtaccatat tggattgtgt atgcccgctc gacggagagc
      721 cgatgaacct cgtgctcctg gcaggcggtc gaagccaacg tatgggtcgg ccaaagtgca
      781 acatggagct ttcaggaaga ctgttgtttg agtatccggt gttggcactt gacgggttgt
      841 gctcgcgctg tatcgtcgtt gcgaatgatg ggtcgatcga tcccggcgac gtttcctgca
      901 tcctcgtgca tgacctcatc gccggcgccg ggcccctcgg tgctatctat accgggctgg
      961 ccgcatccga ttcctggttg aatctcgtag ttgcaggtga catgccgtgc gcgtctccac
     1021 agcttgcggc ggccatggag acttatgctc tgcgcaacgg gctggatatc gtctacccga
     1081 acattgacgg tctgctggaa ccactctttg ccatatactc gaagagagta gtcaccgttg
     1141 ctcgcgcctt gcttgatgct ggacggcgtt ctgtccggga gttgtacgcg agtgcgtcac
     1201 tgagggtcgg tgttgtcgac aggcagttcg taacgcggta tgacaggaag ttgacgtcgt
     1261 ttttcaatgt gaacacaccc gacgacctca tcatggctag actgatgatg cacgtgaggg
     1321 aaaagtctct acaggaggaa tgtgtatgaa catgaacctg gaagatcgtc tgaaagagaa
     1381 ccctgagctc aagaagcagt ttgacctgct cgacgcgtac atcggggagc tggcaacccc
     1441 ggatggcagt ctcatcaacg tcctccaccg tgcccaggag atcttcgggt atctcccgga
     1501 tgaggtgctt gcattcgtct cgggcagact gaatgtgcca cgatcgactg tatacggtgt
     1561 tgtgaccttc taccacttct tctcgcgtgt cccaaaggga cgtcacactg tcagtgtgtg
     1621 ccttggcaca gcatgttatg tgcgaggagg aaacgcagtc tatgagaagg tcatgaagac
     1681 tcttggtatc aaggctggtg agacgaccaa agacggccgt ttcagtctgg atcttgttcg
     1741 ctgtgtaggg gcctgtggcc ttgccccggt cgttgtcatc gacaaggaca catacgggcg
     1801 ttccacgcct gagaaggtcg caacgttgct gaacaagtac gtctaacctc ggagaatggg
     1861 gaggaaacaa tgaaatcact ggaagagctg aagcgcatca aggaacagac tctcaaggag
     1921 atggaccttc gccaggccaa ggagagaggc aaggtcgtcg ttgccatggg gacatgtggt
     1981 attgcagcag gagcgaagga cgctcttgtc gctatcatcg acgaacttga gaaggcggac
     2041 atccatgacg tgaaggtcgt tcaatccggc tgcatgggcc tctgcgacaa ggagccgaca
     2101 gttgaagtat ccatgaagga tcagcctgcc gtcatctacg gcgacgtcaa cgatgcgaat
     2161 gcgcgcagga tcgtccgcga gcacgtccag ggcggcaatg tcgtcgctga tctggtcatc
     2221 aagcgggaga acatctagcc cagggggaga aggtaaaacg caatgatacg aaatcagctg
     2281 ttggtctgtg cgggcggagc ctgcatcacg agtggcgaaa agagcgtcaa ggacgttctg
     2341 gtcgacgaac tgaagaagag tggtatcgag gacgaggtac aggtcataga gaccggatgc
     2401 atgggagcgt gtgacctcgg tccgatcgtc gttgtgtatc ctgagggaac gttctatcag
     2461 aaggtcacag tcaagaatgc ggctctcatc gtcaacgagc atcttgtgaa ggggcgcacg
     2521 gttggcgagt tgctgtacac agagccaacc agtgagaagc cggctcagac cagtggcgat
     2581 atcccattct acacacgtca ggtcaagcgt gttcttcgca actgcggcgc catcgatccg
     2641 ctttcgatcg aggagtacat agcacgtgac gggtatttcg cccttggcaa ggccctcgaa
     2701 atgacacagg acgatatcgt ccagcttctc atcgactcag gactgcgtgg gcgtggcgga
     2761 gcagggtttc ctacgggtct caagtggaag gcgacgcgcg ctgcacaagg ctcaccaaag
     2821 tacgtcgtct gcaacggcga tgaaggtgac ccgggagcat tcatggaccg cagtgtcctt
     2881 gagggagatc cgcactccat cattgaggcc atggccatcg caggatatac cgtcggcgca
     2941 gagaagggct acatgtatgt ccgtgcagag tatccgcttg ccatcgagcg atttgagtat
     3001 gccatcggca aggcgaggga gtacgggttc cttgggaaag gcattctggg cacagagttc
     3061 aacttcgaca tcgagatccg aattggtgcc ggggcattcg tctgtggtga ggagaccgcc
     3121 cttctccggt ccatagaggg caagcgtgga acgccgagcg tcaagccacc gttcccggct
     3181 cagtccgggt tgtggggcaa gccgacattg atcaacaatg tcgagacgta cgccaacatc
     3241 cctcagatca ttacaaatgg tgcagagtgg ttccgcagcg tcggcactgt caagagtccc
     3301 ggcacgaagg tgttcgcact ggcgggcaat gtccagaata ccggcctcgt cgaagtgccg
     3361 atgggcacga cgctccgcga gctcgtcttt gacattggag gcggcatccc cggcggcaag
     3421 aagttcaagg cggcacagac cggaggtcca tccgggggtg tcatcacccc ggactacctg
     3481 gacatgccga tcgactatga cacgctcaag gagatcggga ccatcatggg ctcgggtgga
     3541 ctcatcgttc tcgatgagac aagctgtatg gtggatgtcg ccaagttcta cctcgggttc
     3601 acggtcgacg agtcgtgcgg aaagtgcacg ccatgtcgtg aagggactcg acagatgtac
     3661 cacatgatgg agcagatctg tgatggcaag ggtcgtgatg gtgacatcga gaggctgacc
     3721 aagttgggca agggcatcat tgatacgtcg ctatgcgggc ttggacagtc ggcccccaac
     3781 cctgttcttt cgaccatcag gttctacgag gacgagtacc gcgctcacat ctacgaaaag
     3841 aagtgcccgt cgcatgtgtg caagcatctg ctgacgtact ccatcacacc cgagaagtgt
     3901 gtttggtgca cggcatgtgc gcgacaatgc cccgtccagg ctatcaccgg cgaggtcaag
     3961 aaaactcacg tgatcgatca gagcaagtgc atcaagtgtg gttcctgcta tgaaacctgt
     4021 cgttttggtg cgatccgcaa agagtaattg acccggagga attccatgga gacgagaatg
     4081 ctgaatgtga aaatcaacgg acgggactac caggtgcctg atgggacgac catcctgaag
     4141 gctgccaggg actacgcgaa gatcgacgtg ccgactctct gctaccttga ggggatcagc
     4201 gaagagggcg catgcgccat ctgtgtcgtc gaggtcaagg gtgcccgcaa tctccagcgc
     4261 gcatgtgtcg tcaaggtcag cgagggcatg gaagtgttca ccaacactgc tcgtgtccgc
     4321 gaggcgcgca agctcaacgt cgaactgttg ctggccaatc acccaaagga ctgtttcacg
     4381 tgcgagaaga accagatctg cactcttcgg gagctggccg ccgagctggg cgtgcgtgaa
     4441 gtgcagtttc cacggacgag gactgagtgg ctgccgtttg acaacacgtc attgtcactc
     4501 gtacgtgatc ccaacaagtg cgtgctctgc aggcgctgcg tcaacgtgtg tgccaaggtc
     4561 cagtccgtct acgccatcga tatcgtgggc cgcggctcga acagcagcgt ttccacattc
     4621 ctggatgaag gcctgggcaa cgtcgagtgt gtgaactgtg gacaatgttt gctggtgtgc
     4681 cccaccggcg ctatcaccga gcggagcgaa attccggagg tctggaaggc gctcgggaac
     4741 cccgacaaag tcgtcgtggt cgagacggct ccagcggtgc gtgtcgccat cggcgaggag
     4801 tttggcatgc ccgacgggac gcaggcgact ggaaagatgg cggctgctct caggggcctc
     4861 ggattcgaca aggtgttcga caccgacttc tctgccgatc tgaccatcat ggaagaggga
     4921 ttcgagctta tcggacgtat caagaacgga ggcacactgc cgctgatcac gtcctgctcg
     4981 cccggctggg tcaagttcgt ggagactttc ttcccgaagg ttctcgatca cgtttccacc
     5041 tgcaagtcgc ctcaacagat gtttggcacg gtaacgaaaa cgtactgggc ggagaagatg
     5101 gggatcgatc cgagaaagat cgtcgtcgtc tctatcatgc cgtgcactgc caagaagttc
     5161 gagaaggaac ggcctgagat gaagacggcg ttccactact ggcaggagaa gatgggtctc
     5221 aaggacgaag aggtgttcaa cgacgtcgac tgtgttctga cgactcgcga ggccgcccag
     5281 atgatccgcg aagctggtct cgacttcgca aacctgcctg acgaggattt cgacaagccg
     5341 ctgggtgaat cgacaggagc ggccgtcatc tttggggcca ctggtggtgt catggaagca
     5401 gcgctccgca ctgcatatga agtcctcacc aagaagacgc tggcgaagct ggacttcgag
     5461 gatgtgcgtg gactcgaggg tatcaagacc gccgatgtcg acatcgacgg gctgatcctg
     5521 aaggtcgctg tcgcgaatgg actgggcaat gcccgtgtcc tcatggaaca gatccagaag
     5581 ggcgaatccc cgtatcactt catcgagatc atgtgctgcc ctggcggctg tctcggcggc
     5641 ggcgggcaac ccatctcaca gaaccctgac atcaagcaga ggcgagccga agccatctac
     5701 cgtgaggatg ccggcaagcc catccgcaag tcgcatgaga acccggagat cacacagatc
     5761 tacaaggatt tcctgggtga accgaacagc gagaaagcac atcacttgtt gcacacagaa
     5821 tactgcgatc gcagctgcaa gtagaaccgc atgcatcaag gaggcgttcg cccgtcgcga
     5881 gcgcctccaa cgtcccgaag gggcgagggg aggtacgtca atggacagca cgaagcatgt
     5941 tggagtcatt gccatcatca tcagcaagcg tgagacgacg gccccgaagg tcaaccagat
     6001 actcaccagg catggggacc tcatcatcgg tcgccttgga ctgccctatg gggatcacgg
     6061 cctgcatgtg atctcactca tcgtcgatgg cgagaaggag cagctgcaga gtctgactgc
     6121 tgggctgacc gctgtgccgg atactattgt cgaatcgacg atgagcccgg tctgtttccc
     6181 ctaggcaatt ctgagacagg ggtcacaggg gtgctcggaa ggagcgcgcg tgcgctctgg
     6241 caggcactag acaggcccac tgtccatgtt ggtcagggaa gtggaaaagg attgttgcgg
     6301 caggacaacg tagaaaatcg tttcagcaag gaacacgctc gaaacgatcg gcgctcgagt
     6361 ggtttccatg tgatagagcg cggtcggctt ttgaagggag cactcgtgcc ccgggaaaaa
     6421 aacgatcagg gaggaaacca tggagttgga caacagcact cgaccagccg ggcagacaca
     6481 gccgctgagc gatgaagaca agctttttct gcgtgagctg gtagagacaa atcgcataga
     6541 gcatcatggc cgcttgtcgt acatggacat catgttcgat ctcgagcatc gctacggcaa
     6601 ctccctccca ggctccgtgc ttggcgaggt tgcgcgcctc gtcgagactt cgcctgcaca
     6661 gctgaacggg ttcgtcacct tctacacgat gttgtcgacc actccccggg gaaaacatgt
     6721 cattcgcgtc tgcacaagcg gaccgtgtca tgtgacgggt tcgccggcaa tcttgaatag
     6781 cctcaggcag ttgcttggcg tcgagattgg cgagacgacg ccggatggcg agttcacgct
     6841 tgaaggatcg agttgtctgg gcatctgtgg agtctctccg gcactcacga tcgacgatga
     6901 tgcatatgga aacctcacac ccgaagactt gtcacgtatt cttgatgcaa agcgcgctgg
     6961 aggtgcacaa tgaagttgtt ctcgactcag attgtcgttc ctgtcgactc caactcggtc
     7021 cttcaaggcg cgctcgaagt gaagcatgtt ctcgaggaga ccattgccaa gtacaacctg
     7081 ggcggtcagg tctccgtagt cgaaacgggc gctctcggca tttatgacca gggcgttgtc
     7141 ctggctgtct ttccggatga cgtctactat ggcggcgtga agcctgcaga cattgaagag
     7201 atagtgacag aacaccttct caaggggcgc atcgtgtcac ggctgtcgat tgaccggaac
     7261 ctgtccagca ggcgtctggt cgagaaggag acaggcaaga gccagcagaa gattgtcctg
     7321 agaaatgcag gactcattga cccacagagt atcgacgagt acattgcgcg cgacggttac
     7381 ggagcgctcg ccaaggctct cgcgatgaag cccgaagagg ttgtccaggt cttgaccgac
     7441 agcaagcttc aagggcgtgg cggcgcaggg ttcccgactg gactgaagtg ggggatggtc
     7501 gccaaggagc agagcgacga gaagtacatc gtgtgcaatg cagacgaagg tgagccaggc
     7561 acgttcaagg accgtttgat tctcgagggc gaccctcaca gcatcgtcga agcaatgacc
     7621 atagccggct atgctaccgg ggcacacgtc gggattgcct acatccgcgg cgagtacacg
     7681 atgtcgatag cccggctcaa gaaggctgtc aaggatgcca ctgagtacgg tctgcttggc
     7741 aaggacattc tcggcacggg gttccaattc gatatcgaag tctatcgcgg aggtggagcg
     7801 tacgtctgtg gcgaagaatt cgctctgctc gagtctatcg agaacaagcg cggcacgcct
     7861 cgaaacaagc ctccgtttcc tccacaggtt ggactcttcg ggaagccgac cgtcgtcaac
     7921 aacgtagaga ccttcgccaa tgttcccccg atcctgctca acggcagcga ctggttcaaa
     7981 tccttcggga cgcccacgag cgctggcacc aaggtgttct cgctgtcggg cgacatcgtc
     8041 aaccggggca tcgttgagg
//
LOCUS       DATMZD010000076         2383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_22336,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000076 DATMZD010000000
VERSION     DATMZD010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22336"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1136)
                     /locus_tag="VN478_01600"
     CDS             complement(<1..1136)
                     /locus_tag="VN478_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014452877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWQ21404.1"
                     /translation="MSEQEQLVHEEFDGGRRYDCIVVGAGHAGCEAALVAARAGLQVL
                     VLTGSMDAIAWMPCNPAIGGPGKAQVVREVDALGGEMGLNAERALTQIKLLNTSKGRA
                     VQSVRTQCDKWQYSTSMKQVLEDQPNLTLKQERVERLLTQSGHVVGVVTNYRVRYEAD
                     TVILTTGTYLGGVIHISDIAIPAGRAWDFASSGISDSLRELEFDVQRFNTGTTPRIDK
                     RSVDLNKFVLEEGDRTPLHWSFRSKPRVWEDQLPSYLNWTNPQTIDVTRKYMQLSPSV
                     MGLMVHVGPRTCPSIEEKVRWYPDKTRHQLFLEQEGRSTNEMYVQGMYMSVPLPMQEE
                     ILRTIPGMEHVEIMRPAYAIAYDYVNPQELKLTLETKRVEGLFLA"
     gene            complement(1281..>2383)
                     /locus_tag="VN478_01605"
     CDS             complement(1281..>2383)
                     /locus_tag="VN478_01605"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HWQ21405.1"
                     /translation="TGLPRHDKVFAGHTDLTRQQIMESLRWLEPSCKPRTRFQYQNMM
                     YAVAGLIIEAADSRPWEDFVQAELLEPLGMNRTRILRKAALATGDYAMPYAVRDDNLV
                     RMGIESLTPGMRPAGGIISTAEDMGRWLIFQLGDGTIDGKRILTTETLHRLHTPVIDV
                     TGDERSFVGLPEFGDGSYCLGWNCQSYRGHTMLHHSGGNDGLTSTILLFPDDRVGVSV
                     LTNSDDTMQRLVPALSAADLLLNLDPLPWNERWLERVHRRQAQTSQAPAPVPMDPGRP
                     LSAYVGSYTHPAYGQLGVTETTSGLHMTLHSLNADLTGSAPDTFAAAAGPILDPGDTV
                     HFDMDAAGCAASVAAPLEHAVAPILFVRTRAA"
BASE COUNT          396 a          729 c          778 g          480 t
ORIGIN      
        1 gcaagaaaca gtccctcgac acgctttgtc tccagggtca gcttcagttc ctgcgggttg
       61 acatagtcgt aggcgatggc gtacgccggc cgcatgatct ccacgtgctc catgccgggg
      121 atcgtacgca ggatctcttc ctgcatgggc agagggacgc tcatgtacat gccctgcacg
      181 tacatctcgt tggtgctgcg gccttcctgc tcgaggaaca gctggtgccg ggtcttgtcg
      241 ggataccagc gtaccttctc ctcgatactg ggacaggtgc gcgggccgac gtggaccatg
      301 agtcccatga cggaaggaga cagctgcatg tatttgcgcg tgacgtcgat cgtctggggg
      361 ttcgtccagt tcaggtagct gggcagctga tcttcccaga cgcgtggctt gctgcggaag
      421 gaccagtgca ggggggtcct gtcgccctcc tccaggacga acttgttcag gtcgacgctc
      481 cgcttgtcga tgcgcggcgt cgtgccggtg ttgaagcgct ggacgtcaaa ctccagttcc
      541 ctgagggaat cgctgatacc gctgctggcg aagtcccagg cgcgccccgc ggggatggcg
      601 atgtcgctga tgtgaatgac gccgcccagg tatgtgccgg tcgtcaggat gacggtgtcg
      661 gcctcatagc ggacgcggta gttggtgacc acgcccacaa catgacccga ctgtgtcaac
      721 aggcgctcca cccgctcctg cttcagcgtc aggttgggct ggtcctccag cacctgcttc
      781 atgctggtgc tgtactgcca cttgtcgcac tgcgtgcgca cgctctggac ggcgcgtccc
      841 ttagaggtgt tgagcagctt gatctgcgtc agcgcccgct cggcgttgag tcccatctcg
      901 ccccccaggg cgtcgacctc acggacgacc tgcgctttgc ccggacctcc gatagctggg
      961 ttgcagggca tccaggcgat ggcgtccatg gaaccagtca gaaccaggac ctgcaatccg
     1021 gcgcgtgcag cgaccagcgc tgcctcgcat ccggcatggc ctgccccgac gacgatgcag
     1081 tcgtaccgcc ttccgccgtc gaattcctcg tgtaccagct gttcttgttc gctcatcggt
     1141 ccatcccctg ttcctcctgg tgaagacaga gataggatag cacgggttgc gatctcactg
     1201 tgcagccttc agcagatctc accgcaaaac cccgccgcga gcgcggcggg gtctgatact
     1261 ctctggcgtg ccgggctgtc tcaggctgcc cgggttcgca cgaacaggat aggcgccact
     1321 gcgtgctcca ggggggctgc aacacttgca gcacatccgg ctgcgtccat gtcgaagtga
     1381 accgtgtccc cggggtccag gatgggaccc gctgcagccg caaacgtgtc tggagctgat
     1441 ccggtcaggt cggcgttcag gctgtgcagt gtcatgtgca gtcctgaagt tgtctcggtc
     1501 acgcccagct gcccgtatgc agggtgagtg tagctgccca cataggcgga cagcggcctg
     1561 cccgggtcca tgggcacggg cgcaggggcc tgagaagtct gtgcctggcg acggtggaca
     1621 cgctcgagcc agcgttcgtt ccatggcagc gggtcgaggt tcagcaggag gtcggcggcg
     1681 gacagcgcag ggaccagtcg ttgcatcgtg tcgtcgctgt tggtcaacac agagacgccg
     1741 acacggtcgt caggaaacag caggatggtc gaggtcaggc cgtcattgcc cccggagtgg
     1801 tgcagcatcg tgtgtccccg gtaggactgg cagttccatc caaggcaata ggacccgtcg
     1861 ccgaattcgg gcagaccgac gaacgagcgt tcgtcccccg tcacgtcgat gaccggcgta
     1921 tggagccgat gcagggtctc agtcgtgagt attctcttcc cgtcgatcgt accgtcgccg
     1981 agctggaaga tgagccatcg gcccatgtct tcggcggtcg agatgatgcc cccggcgggc
     2041 cgcatacccg gagtgagcga ttcaatgccc atgcggacga gattgtcgtc ccggacagcg
     2101 tagggcatgg cgtagtcgcc ggtcgccagt gctgccttac gtaatatccg cgtacggttc
     2161 atgccaagtg gctcgaggag ttcggcctgg acgaagtcct cccacggacg actgtccgcc
     2221 gcttcgatga tgaggccggc gacggcatac atcatgttct ggtattgaaa gcgagtacgc
     2281 ggtttgcagc tgggttcgag ccagcgcagg ctctccatga tctgctgccg ggtcaggtcg
     2341 gtgtggccgg caaatacctt gtcgtggcgc ggcaatccag tgc
//
LOCUS       DATMZD010000077         4790 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_12348,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000077 DATMZD010000000
VERSION     DATMZD010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4790
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12348"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="VN478_01610"
     CDS             complement(<1..389)
                     /locus_tag="VN478_01610"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWQ21406.1"
                     /translation="MTLEGKTALVTGGSRGIGRGIALELAARGASVIIDFHRNGEAAQ
                     EVVEAIVAMGRVCEAIQTDVGEPAEADRLVAQSIRLTGSLDILVSNAGITRDALLVRM
                     TDEKWNEVLRTNLTGAFNITRAVARHM"
     gene            complement(386..1285)
                     /gene="fabD"
                     /locus_tag="VN478_01615"
     CDS             complement(386..1285)
                     /gene="fabD"
                     /locus_tag="VN478_01615"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HWQ21407.1"
                     /translation="MRAFLFPGQGSQYIGMTSRAADPVATRRVLERANDALGFDLWRL
                     IETGDEATLTRTENTQPALFATEMAWVEGLSIRGVICDVAAGHSLGEFSALCCSGVFT
                     FEDGIRLVRRRGEIMAEAASRSPGTMAAVVGLNESDLEQALVEGRRSGIVEAANYNAS
                     DQTVVSGEVAAVERVMAAVKALGRGRAIKLRVGAPFHSSIMTHGAEEFGAVLREMTFS
                     RPRFAVVNNVAAQVEYEPEVIKASLVAQFRSPVQWVRTMQTLRDMGVSEYLEVGPKGV
                     LTALAGKAVPGANATAVEEEKWQ"
     gene            complement(1597..3117)
                     /locus_tag="VN478_01620"
     CDS             complement(1597..3117)
                     /locus_tag="VN478_01620"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HWQ21408.1"
                     /translation="MDKHVGTDSTGETDGLFEVKSLLAAVEQPSIPGLDAAEEKAPEQ
                     VAVPVEAEAEPVKATADETGTSELEAALVADLSPKAGYAPRLLRRGDVIDVSVVKLER
                     DGVMVNAGGKGDYYIPLNRLTTRTDAAVGEIAHVGETMKVAVVKEEDEQGTAVLSKLK
                     ADQEEVWTDVEKYFDEQTIVTAKVTKAIKGGLLVDIGVPCFLPQSQVASDYRNNLPSL
                     VGQEIPVKVTEFDKKLRRIIASHRAAMQVIRQKQKKEFFTKIQPGDIYEGTVNGIAEF
                     GAFVDIGYGVEGLVHLSELTYGRRRPVEEVVQKKDKVKVKVLKVDPETGKVSLSIKQT
                     KPHPWETIEEDYPVGMNLEGAVIRILTFGAIVELSEGVTGLLHISQISNDKIRKVEDV
                     LNVGDVVKVRVLEVLKDERKVKLSMKGVEGNEGLFGREPGQGKPATELTSDSDATDST
                     PVEPNAVEPNAVEPEVIEPEVVEAEVVESEVPAAENVETAEDEAVSTEEKEVPPEA"
     gene            complement(3444..3821)
                     /locus_tag="VN478_01625"
     CDS             complement(3444..3821)
                     /locus_tag="VN478_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005811508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HWQ21409.1"
                     /translation="MTQQMETQDIKAESNTSKRIVGVIFAVVEVVLAFRLIFKGLGAN
                     PSSGFVRGIYGVTQFLVGIFEGIFSRGTAGGAETTAVFEPATLIVMVIIGLIAWGVLR
                     LMTPHTGTRVERTETTGRDTQGK"
     gene            complement(3944..4093)
                     /locus_tag="VN478_01630"
     CDS             complement(3944..4093)
                     /locus_tag="VN478_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HWQ21410.1"
                     /translation="MLWTIAVILVVLWILGMVSSYTLGGLIHILLVLAVIAVVVNLIR
                     GNRHI"
     gene            complement(4249..>4790)
                     /gene="recR"
                     /locus_tag="VN478_01635"
     CDS             complement(4249..>4790)
                     /gene="recR"
                     /locus_tag="VN478_01635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HWQ21411.1"
                     /translation="PKAADRLVFYLLDQPEEDVRSLAESLVAAKREIAFCKRCGNFAQ
                     GDLCEVCQDSSRDRKLLCVVEEIRDLSAIEKTGAYRGLYHVLHGRIDPIARVGPGELQ
                     LDHLVERIRSDDVREVIVATNPNFNGDITAMYIAKLLCGSKVRVTRIATGIPKGSDLE
                     FADVFTLTQALEKRTDVQS"
BASE COUNT          995 a         1547 c         1206 g         1042 t
ORIGIN      
        1 atcatgtgcc gcgcgacggc gcgggtaatg ttgaacgccc ccgtgagatt cgtccgcaac
       61 acctcgttcc acttctcgtc cgtcatccgg accagcaaag catctcttgt gatgccggca
      121 ttgctgacca ggatgtccag cgatcctgtg agacgaatac tctgtgctac gagacggtca
      181 gcctcggccg gctccccgac gtctgtctgg attgcctcac agacccggcc catggcgaca
      241 atggcctcga cgacttcctg agcagcctcg ccgttccggt ggaaatcaat gatcacgctg
      301 gctccccgcg cagccagctc aagggcgatg cccctgccga tgcctcgtga cccccccgtg
      361 accagggccg tctttccctc aagcgtcatt gccacttttc ctcctccacg gctgtggcgt
      421 tcgccccggg tacagctttg ccggcaagcg ctgttagcac acccttcggt ccgacttcca
      481 ggtactcgct cacgcccatg tcccgtagcg tctgcatcgt gcgcacccac tgcactggac
      541 tcctgaactg ggcgacaagc gacgccttga tcacttcggg ttcatattcc acctgtgctg
      601 caacgttgtt gacgactgcg aaacggggac gggagaatgt catctcgcgc aagacagctc
      661 caaactcctc tgcgccatga gtcatgatgg atgagtggaa gggagctccc acccgaagct
      721 tgatggccct tcctctgccg agagccttca cggccgccat gaccctctct acggcagcta
      781 cttcgccgga cacgaccgtc tgatccgaag cattgtagtt cgcagcttcc acgattccgg
      841 atcgacgtcc ttctacaagg gcttgttcaa ggtcgctctc gttcagcccc acgacggcgg
      901 ccatcgtacc tggtgacctg gacgccgcct ctgccatgat ctcgccacga cgccggacga
      961 gtcgaatgcc atcctcaaac gtgaagactc cagaacaaca gagcgcactg aactctccaa
     1021 ggctatggcc cgctgcaacg tcacagatga caccccggat cgacagcccc tcgacccacg
     1081 ccatctcggt cgcaaagagt gcaggctggg tgttttcggt acgagtaagt gttgcctcgt
     1141 cacctgtctc gatgaggcgc cacaggtcaa aacctagtgc atcgttggcc cgttcaagca
     1201 ctctgcgagt cgctaccgga tctgccgctc gactggtcat tccaatgtac tgcgatccct
     1261 gcccgggaaa cagaaatgct ctcatgctcg cacctcctcc ttccttctga cactcgctga
     1321 accgctaccc acgcccaatg gctgtggccg gagtctggca cgatcgactc ttccctgctc
     1381 tcagaacatt cgtgaagtcg accttgtcgt cacgataccc gcagtgacgc ccgccaaagg
     1441 aaactggtcg agaacccggg gcctcgccct cgtcaatcat gtaaaatgga cggagtgcag
     1501 acgctcagac acctgaacga tactgcactc cgccgatctc aagctatctg gaactgctcg
     1561 tctggaaccg cacagcaggc gaaccagaat cgttgatcag gcctctgggg ggacctcttt
     1621 ctcttcagtc gacacagcct cgtcttctgc agtctcaacg ttctcagcag cggggacttc
     1681 actctcaaca acctcggcct cgacgacctc aggctcgata acctcaggct caacagcatt
     1741 gggctcaaca gcattgggct caacgggcgt gctgtcagtc gcatcgctgt ctgatgtcag
     1801 ttctgtggcc ggcttgcctt gcccgggttc acgtccaaac agaccctcgt tgccctccac
     1861 acccttcatg ctgagcttga ctttgcgctc atccttcagc acctcgagta cccggacttt
     1921 gacgacgtct ccgacgttca acacatcttc gaccttgcga atcttgtcgt tggagatctg
     1981 agagatgtgc agaagtccgg tgacgccctc tgagagctcg acgatagctc caaaggtcag
     2041 gatacggatg accgcgcctt caaggttcat gccaacgggg tagtcctcct cgatcgtctc
     2101 ccagggatgc ggtttcgtct gcttgatgct cagggacacc ttccccgtct cagggtcgac
     2161 ctttagcacc ttgaccttga ccttgtcctt cttctgaacg acttcctcga caggacgacg
     2221 acgaccatag gtcaactccg agaggtggac cagaccctca actccgtatc cgatatctac
     2281 aaatgcacca aactcggcga tgccgttgac agtgccttcg tagatgtctc ctggctggat
     2341 cttcgtgaag aactccttct tctgcttctg ccggataacc tgcattgcgg cacgatgaga
     2401 agcgataatc cgacgaagct tcttgtcgaa ctccgtgacc ttgacaggaa tctcctgacc
     2461 gaccagcgaa ggcaggttgt tccggtaatc gctggcgacc tgcgactgcg gcaggaaaca
     2521 gggcacgccg atgtccacaa gcaagccacc cttgatagcc ttcgtgacct tcgcagttac
     2581 aattgtctgt tcgtcgaaat acttctcgac gtcagtccag acctcttcct gatcagcctt
     2641 caacttggaa aggactgcgg tgccttgctc gtcctcttcc ttgacgacgg ccaccttcat
     2701 cgtttcgcca acgtgcgcga tttcgccaac agccgcatcg gtgcgcgtag tcaagcggtt
     2761 cagtggaatg tagtagtctc ccttgccacc agcgttgacc atgactccgt cgcgttcgag
     2821 cttgacgaca gatacgtcga tgacgtctcc gcgccgcagc aaacgtggtg catagccagc
     2881 cttgggagac aaatctgcga caagtgccgc ctcgagctcg gaggtgccgg tctcatcggc
     2941 tgtcgccttg acgggttcgg cctctgcctc caccggcaca gccacttgtt caggagcctt
     3001 ttcctctgcc gcgtcgagac ccggtatgct gggctgttcg actgctgcaa gcagactctt
     3061 gacctcaaac agtccgtccg tttcacctgt gctgtctgtt ccaacgtgtt tgtccatgtc
     3121 caccatgttg acccctctac ataaggatga atgtaaatca gtctttgagt ataaagcgaa
     3181 aacgaatgct tgtcaaaccc tcgaaaaccg cgtcagtacc cgctgacgct ggattcgcaa
     3241 gccacggaca cttgcgccga aagatcgacc gctctgccat cctccggatg ggtcctgctc
     3301 ctcagcgtcc cgatctccgc gccggaataa tcgacaccac gagaacctgt caagcaggtg
     3361 cgacaggtcg gcaaacatcc cgacctgtcg caaagccaga cgacatgagc acagcgaaga
     3421 ctcgcaactc gcttatcgag tgctcacttc ccctgtgtgt cacgcccagt ggtctccgtc
     3481 ctttccacac gagtccctgt gtgaggcgtc atcaacctca ggactcccca tgcgatcagg
     3541 ccgataatca ccatgacgat gagggttgca ggttcaaaga ccgccgtagt ttctgcgccg
     3601 ccggctgtgc cccgggagaa gatgccctca aaaatgccta caagaaactg agtgacacca
     3661 tagatgccac gtacaaagcc gctactcggg tttgccccaa gtcccttgaa aatcagacga
     3721 aaagccagaa cgacttcgac gacggcgaag atcacgccga cgattcgctt cgaggtattg
     3781 ctctccgcct tgatatcctg cgtctccatt tgctgggtca ttgtcccacc tcccgtgaat
     3841 cacgctgact tgattggcaa gaagaaaccg tcttccgcgg aatatgcgac atacggccgg
     3901 cataccaatc gcggcgctcg tgcgccgaaa cgggccttcc ctgctagatg tgtctgttcc
     3961 cccgaatgag gttcaccacg actgcaatga cagccaggac cagaagaatg tggatcaaac
     4021 cgcccaaagt gtacgaactg accatgccca gaatccacag gaccacgaga attacggcga
     4081 tcgtccatag catagcttgt cacttctcct ttgcagatac cgtgcgcatg aggcttgcgc
     4141 gctctggcta ctctggtggt tacaacttag tttattatac caccaaatcg gagaacgact
     4201 attccatcaa gtgaactggt cttcacgagg caccggcgag ggtttggctc agctctgaac
     4261 gtcggtcctc ttctcaagtg cctgagtcag agtgaacacg tcagcgaact ccaagtcgct
     4321 tcccttggga atacccgtcg caatacgcgt cacacgcacc ttcgacccgc agagcagttt
     4381 cgcaatatac atggcagtga tgtcgccatt gaagttgggg ttcgtcgcga cgatgacctc
     4441 gcgcacatca tccgaacgta ttcgttcgac aaggtggtcc agctgcagtt ccccgggtcc
     4501 cacacgggct atcgggtcta tccgtccatg cagaacgtgg tacagacccc ggtaggcccc
     4561 tgtcttttcg attgcagaga ggtcgcgtat ctcctccacg acgcatagca gcttcctatc
     4621 ccgcgacgag tcctggcaga cttcgcagag atcaccctga gcgaaattgc cgcaccgctt
     4681 gcagaaggct atttcccgct ttgctgccac cagagactcc gcaagggagc gcacatcctc
     4741 ctcgggctgg tcaagcagat agaatacaag cctgtccgca gccttcggcc
//
LOCUS       DATMZD010000078         2797 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_112774,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000078 DATMZD010000000
VERSION     DATMZD010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112774"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VN478_01640"
     CDS             <1..264
                     /locus_tag="VN478_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21412.1"
                     /translation="GWLKPQVPCHVRPLVVFARAKVRAGVSVNGVRVISLSSLTSAIM
                     GRAPSVSAEDVRRYANCLGGIGATMAARSGRRSKGPGERVGVL"
     gene            complement(320..892)
                     /locus_tag="VN478_01645"
     CDS             complement(320..892)
                     /locus_tag="VN478_01645"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HWQ21413.1"
                     /translation="MILVGTICTILALVYAGIDLYGHKQEPDRADVICVFGAAVWGDT
                     PSPELEARLMWAVRLYRQGRAPVLFLSGGTTGSSMTESDVMGSVAIEQGIPQSALVFD
                     DAGTTTARTLTNLKRYMQGNGLSSCLMVSSPFHMARIMVLSRLDGLKAFTSPPDETPV
                     SISATQRARATAREELALFKDLLVFAVSPD"
     gene            1010..2611
                     /gene="dnaX"
                     /locus_tag="VN478_01650"
     CDS             1010..2611
                     /gene="dnaX"
                     /locus_tag="VN478_01650"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR02397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HWQ21414.1"
                     /translation="MEPTERYVSLYRKYRPTTFDSVIGQETVVRILKGSVDTGRTSHA
                     YLFAGPKGTGKTTVARILAKALNCDKGPNSHPCMECEHCRAITSGTDVDVIEIDAASN
                     RGIDEIRDLRESTRFVPARSRRKIFIIDEAHMLTIQAFNALLKTLEEPPEYVMFILAT
                     TEPQKLPETILSRCQRFDFKRIGETAMFQYLQGLRSVEAPNVTDDALHQIVALSGGSM
                     RGAIGLVDQFSVFGTESVSGATVLDLLGLPTEENLALLVEALLSHDPKGVTSVLDQLE
                     RSGIEPADLLERSIALISRYIVDKVTMTPSTLEGIPADHLKAFPDLVLVNLAGTFARI
                     QQQSVYLSDPYPLVQAFLLAMALGLPMSLDHPRDDVSHGVQFTSIQDVPRPALSQAET
                     MQNALGLSPDSPPGVAPQSLGTLEGEPAERWGHFKALVSEASRPIGVMLQALDVREVG
                     VDQGALRIVLGPKTKLFRAMLEQKQDEVLVPAARSVYAVDRVNLVDEGSQQAVREETV
                     TEAPVDDKLELLKKTFDATESLFGS"
BASE COUNT          575 a          791 c          854 g          577 t
ORIGIN      
        1 ggatggctga agccgcaagt tccatgtcac gtccgacccc ttgtcgtctt tgccagggcc
       61 aaggtccgtg ccggcgtgtc agtcaatggt gtacgggtca tctcgctctc ttcgcttacc
      121 agtgcgatca tgggtcgggc gccgtccgtg tcggccgagg atgtccggcg atacgcgaac
      181 tgtctcgggg gtatcggcgc caccatggct gcccggtcgg gaaggcgaag caagggaccc
      241 ggggagcggg tcggagtact gtagtcgcca gcattcagcg ccagactcct ctccgcgtca
      301 tgccggcgcc gtgttcccgc tagtcaggcg acacggcaaa gacaagcaga tccttgaaca
      361 gggcaagctc ttcacgagcg gttgccctgg cccgttgcgt tgcactgatc gagactggcg
      421 tctcgtcggg cggactggtg aacgccttca gaccatccaa tctggacaac accatgatac
      481 gggccatgtg gaagggagac gaaaccatca agcaggaaga cagaccgttt ccctgcatgt
      541 agcgtttgag gttcgtgagt gtgcgcgccg tcgtcgtgcc tgcgtcgtca aataccagag
      601 cactttgggg aatcccctgt tcaatggcga cagatcccat gacatcggat tcggtcatgc
      661 ttgatccggt ggtgcccccc gagaggaaca gcaccggcgc acgaccttga cggtacagcc
      721 ggactgccca catcaagcgt gcctccagct ctggagaggg cgtgtctccc cacacggcag
      781 ctccgaacac gcagatgacg tcagcccggt cgggctcctg cttgtgtcca tacagatcta
      841 tgccggcata caccagagcg agaatcgtgc agatcgtgcc aacgagtatc accgctgtcc
      901 agaagaatcg tctcacctga acagtatagg ccgtgaccct gccgcctcaa gcggcatggc
      961 tccgggcttt gcttgcaatg acatgtgcgg gcaatatact actggcacca tggaacccac
     1021 tgagcgctat gtttcgttgt accggaagta ccgtccgact acgttcgaca gtgtcattgg
     1081 tcaggagaca gttgtacgta tcctcaaggg gtcggtcgac actggcagga cctctcacgc
     1141 atatctgttt gcgggcccca agggcacggg caagacgacc gtcgcccgta ttctggccaa
     1201 ggccctgaac tgcgataagg gcccaaacag tcatccttgc atggagtgtg agcattgccg
     1261 ggcgatcacg tctggcacgg atgtcgacgt cattgagatt gatgcggcca gcaatcgggg
     1321 catagacgag atcagggacc tgcgggaatc cacaaggttc gttccggctc gtagccggag
     1381 gaagatcttc atcatcgacg aagcgcacat gctcactatt caggcattca atgcacttct
     1441 gaagacactt gaggaaccgc ctgagtatgt catgttcatc cttgcgacga ccgaacccca
     1501 gaaactgccc gagacgattc tgtcccggtg ccagcggttc gacttcaagc ggatcggcga
     1561 gacggctatg tttcagtacc tgcagggtct tcggagtgtt gaggctccga atgtcacaga
     1621 cgacgctctt caccagatcg ttgcgctgag cggcggctcg atgagagggg cgataggtct
     1681 agtcgatcag ttctcggtgt tcgggaccga atcagtatct ggggccacag tactggactt
     1741 gctcggactg ccgaccgagg agaatctcgc cctgctggtc gaagcgctcc tctcccatga
     1801 tccaaaggga gtcacgtctg ttctggacca gctggagcgg agtggcatcg aacctgcaga
     1861 tctgctggag aggtcaatag cccttatcag cagatatata gtcgacaagg tgaccatgac
     1921 gccttcgaca ctggaaggta ttccagccga tcatctgaag gcattcccag accttgttct
     1981 agtgaatctt gctggcactt ttgcgcgaat ccagcagcaa tcagtttatc tgtctgaccc
     2041 gtatcctctt gtccaggcct tcctgctcgc catggctctg gggctgccca tgagtctcga
     2101 ccatccgcgt gatgacgtct cccatggggt gcagttcacg agcatccagg acgtgcccag
     2161 acctgctctt tcccaggcag agaccatgca gaatgctttg gggttgtcac cggatagccc
     2221 cccgggcgtg gcaccgcagt ccttgggaac gttggaaggg gagcccgctg agcggtgggg
     2281 acacttcaag gctctcgtgt ctgaggccag caggcccatt ggcgtcatgc ttcaagcgct
     2341 cgatgttcgt gaggttgggg tcgaccaggg tgctctgagg attgtcctgg gacccaagac
     2401 caagctgttt cgtgccatgc tggagcagaa gcaggacgag gttcttgtcc ccgcagcgcg
     2461 gtctgtctac gcggttgacc gggtgaatct ggttgacgaa ggttcccagc aggctgtgcg
     2521 cgaggagacc gtcaccgagg ctccagtgga cgacaagctg gaactgctta agaagacgtt
     2581 tgatgcgacg gagtcgctgt tcggctcctg aggcgccctg ggaggagcag catgtacaat
     2641 gtcagcaatt tgatgaagca agcccagaag atgcaggagg atgcggcacg tgccgaacag
     2701 gagctcctgg atcgcaggat atctgccacg tcgggcggag gagctgtgac tgcggtagtg
     2761 aatggcaaga aagacatcat tggcatcagc atccagg
//
LOCUS       DATMZD010000079         2813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_32608,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000079 DATMZD010000000
VERSION     DATMZD010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32608"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="VN478_01655"
     CDS             <1..352
                     /locus_tag="VN478_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21415.1"
                     /translation="DPGIYYSDGHLMDGNKPLQLLPFDSVCLRKTMGDDVELLGTTTH
                     LFPGLDIASFHSHERSIVLRQSDSARLEGEALIGVPDGSDGWTVNGAPIAPERQGARM
                     VLRVPLMKVALPVD"
     gene            396..1694
                     /locus_tag="VN478_01660"
     CDS             396..1694
                     /locus_tag="VN478_01660"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HWQ21416.1"
                     /translation="MEPFSLPENFLLGTATASLQIEGGDRNNSWYRWVQTGHVKDGTS
                     CIVADDHWNRVPDDIALMKQLHQHTYRLSLEWSRIEPIRGQFDKDAMAHYRLEIQQLA
                     NAGIRPLVTLHHFSNPLWLEDAGAWINPEVVDLFERYTAYVVENLGDLVSDWCTINEP
                     NVYLAQGYVTGGWPPNDISITKYFRGARNMILAHIKSYRKIHQIRASNGRDDTMVGVA
                     NHLRLFDPKHGNGRERWAAGMYERLFQDLFVSGMCEGRLLSPLGSGYPLGEGRYVDFL
                     GVNYYTRDIVDCSAVSANPLGKLEVREDSQTNDLGWELYPEGLYRVCKKYWDRYHAPV
                     FITENGTADAKDAFRTRYIYDHLLQVSRLIEDGVDVQRYYHWSLMDNFEWIEGLSAKF
                     GIVAVDYATLERTVRTSGWFYADVAKHHGVTEKMIQQFFS"
     gene            1899..>2813
                     /locus_tag="VN478_01665"
     CDS             1899..>2813
                     /locus_tag="VN478_01665"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HWQ21417.1"
                     /translation="MSNALAVCFVWHFHQPQYQDLTTPQAVLPWARLHCARNYTMMLH
                     LLEEQPAAHVTINITPVLATQLDAYAQGTCTDEWLELSAARVADLDMEQRSRIVAMFF
                     DVNLERIIQQDFRYRQLALRRSDPIGTWSEQELRDLQCLFTRAWTCSSELRKRPRGTA
                     LLAKARDFTEEDRVELVGIQHDLVRGFLPRLGALCRSGQVEVTTSPYAHPILPLISDT
                     DSARAVQDTPLPHPPFRHPEDAQQQLQLGKRYVEQLLQVSISGCWPSEGSVSPEAVVE
                     IARAGFSWTATDEDILLRELPGEPRSALY"
BASE COUNT          599 a          867 c          801 g          546 t
ORIGIN      
        1 ggacccaggg atctactact cggacgggca cctgatggac ggaaacaagc cgctgcagct
       61 cctgcccttc gattcggtgt gcctccggaa gacgatggga gatgacgtcg aactcctcgg
      121 aacgacgacc catctcttcc ctgggctcga catcgcgagc ttccactcac acgaacggtc
      181 catcgtgctt cgacagtccg acagcgcccg gctcgagggg gaagctctga tcggtgtccc
      241 cgacggatcc gacggatgga cggtgaacgg ggcgcccatc gccccagaac gtcaaggtgc
      301 ccgaatggtg ctgcgcgtgc cccttatgaa ggttgcgctg ccggtcgatt gatacatact
      361 gctgacagcg gcatcacggg acaaggaggt agaccatgga accattttcg ttaccggaga
      421 acttcctgct gggcactgca acagcttcgc tgcagatcga gggtggtgat cgcaacaaca
      481 gctggtaccg atgggttcag actggtcatg tcaaggacgg caccagttgt atcgtggctg
      541 acgatcattg gaacagggtg cctgatgaca tagcactcat gaagcagctg catcagcaca
      601 cctaccggct gagtctggaa tggagccgca tcgagccgat acgggggcag ttcgacaagg
      661 atgccatggc ccactatcgt ctcgagatcc agcagttggc taacgcggga atcagacctc
      721 ttgtcacgct gcatcacttc tcgaaccctc tgtggcttga agacgcgggc gcatggatca
      781 acccggaagt cgtcgacctg tttgagcggt acactgccta cgtcgtcgag aacctcggtg
      841 acctcgtgag cgactggtgt acgatcaacg agccaaatgt ctacctcgcc caggggtacg
      901 tcactggggg atggccgccc aacgatatca gcattaccaa gtacttcagg ggcgctcgca
      961 acatgatcct ggcacatatc aagtcctatc gcaagatcca tcagatacgt gcttcgaatg
     1021 ggcgcgacga tacgatggtc ggtgtcgcca atcacctcag gctgttcgat ccgaagcacg
     1081 gcaacgggcg cgaacgctgg gccgctggca tgtatgagag gctcttccag gacctgttcg
     1141 tctccgggat gtgcgaggga agactcctat ccccgctggg cagcggctat cctcttgggg
     1201 aaggacgcta cgtcgacttc ctcggggtca actactacac ccgagacatc gtggattgtt
     1261 ccgccgtctc ggcgaatccc ctgggcaagc tcgaggtacg cgaggacagt cagacaaacg
     1321 accttggatg ggaactgtac cccgaggggc tgtaccgcgt gtgcaagaaa tactgggacc
     1381 gttaccacgc ccccgtcttc atcactgaga atggcacagc cgacgccaag gacgccttcc
     1441 gcaccaggta catctacgac caccttctcc aggtgagccg gctcatcgag gacggcgtcg
     1501 acgtgcaacg gtactaccac tggtctctga tggacaactt cgagtggata gagggcctga
     1561 gtgccaagtt cggcatcgtt gctgtagact atgccaccct ggagagaacg gtgcgcacca
     1621 gcggatggtt ctatgctgac gtcgcaaaac accacggcgt cacggagaag atgatccagc
     1681 agttcttcag ctgaacccct catgtatgcc acgaacgcgc cgggaccaaa cccggcgcgt
     1741 tcgtcttctc ctgccgccct ctcctgccgc aaccacatgc agtgaatgac tttcctcgtc
     1801 gattccctat actacggtga agagaacatg agacagaaaa tgatgctgag aacatacgag
     1861 gatcgactgc gccgcaggcg ccttcgagct atctgaccat gtcgaatgcc ctggccgtct
     1921 gtttcgtctg gcattttcac cagccccagt atcaagacct gaccacgcca caggctgtac
     1981 tgccctgggc gcgtcttcac tgtgcccgca actacacgat gatgcttcat ctgctcgaag
     2041 agcagcctgc tgcgcatgtc acaatcaaca tcacgcccgt tctcgctacg caacttgacg
     2101 cttacgccca aggcacctgc acagacgagt ggcttgaact atctgcggct cgagtcgccg
     2161 atcttgacat ggagcaacgg tcgcgaatcg tcgcgatgtt ctttgacgtc aatctggaac
     2221 gaatcatcca gcaggacttc cgctaccgcc aactcgccct gcgcaggtct gatccaatag
     2281 gcacgtggtc ggaacaggag ctgcgggacc tccagtgcct gttcacgcga gcttggacgt
     2341 gctccagtga actcaggaag aggcccagag ggacagccct cctggcgaag gcccgcgatt
     2401 tcaccgagga ggacagagtc gagctggtcg gcatccagca cgacctggtg aggggctttc
     2461 tcccacgact tggggcactc tgccgatcag ggcaggtcga ggtcactacg tcgccctacg
     2521 cacaccccat ccttccgctc atcagcgaca cggactctgc acgcgctgtc caggacactc
     2581 ctctcccaca tccaccattc cgtcaccctg aggatgcaca gcaacagctg cagcttggaa
     2641 agcgctatgt cgagcagctc ctgcaggttt ccatctcagg ctgctggcct tccgaaggga
     2701 gcgtatcgcc ggaagcggtt gttgagatag cgcgcgccgg cttctcctgg acggccacgg
     2761 acgaggacat cctcttgcgg gagcttcctg gagaaccccg gtcggcactg tac
//
LOCUS       DATMZD010000080         2652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_42490,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000080 DATMZD010000000
VERSION     DATMZD010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42490"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1302)
                     /locus_tag="VN478_01670"
     CDS             complement(<1..1302)
                     /locus_tag="VN478_01670"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWQ21418.1"
                     /translation="MLADGKYQTVCAYDCPDSCGMIGTCKEGRLMELEGREDNQYTRG
                     FVCHKGRRWVKELRGRDRLTSPMVRKNGKFIGVSWDEALDMTATAIRSAVEHHGHQSF
                     LFYEGSGNLLIGNRMQRLLPLLLGGGTMAHGSLCGFEGKVGLARSYGTVGRDQPLAAL
                     ESKSILLWGRNPAENSVHFLAILHDARRKGARLGTIDVRATPTTAASDSAWIIRPGSD
                     LTLALYLCHEAILLHGEPVAPHEGFEVFREGCMLVSAEDAEQATGLSAEALHALVGFV
                     MDQPPLSVWTGYGLQRTRWGADIVHTIDALGFLTGNHGIPGGGVWFDQGDDDLLPDDL
                     VDVPGADPRFLWRPNLGASLLEADPPIEAAMFVRGNPASQCPDAGKVLAFLKHCPFSV
                     CLDWHMSVTARACTLVLPVSVFLERGGDYVMSYLHDLLQKTA"
     gene            complement(1432..2442)
                     /locus_tag="VN478_01675"
     CDS             complement(1432..2442)
                     /locus_tag="VN478_01675"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HWQ21419.1"
                     /translation="MSQAKERLSELRDRGLITSEQYDELIAALDSVEPDVPAAMIPQV
                     PAVPQPPSQPASPRMTIHIDTGDQSRASQSAIGTTGSRLMVRLISEDLRVRGVPGLTS
                     VRVVNGQSSVHTGLNGDTVEIESALTGGEFHGFSLHFGSDSGDTVELEIPENMPCELK
                     TVSGDISLENLRGALNVRSVSGDIDGRSLTHVQSATSTSGDLDLEQCLVDGDVVTKSG
                     DVEMDSCTVHGMLKSYSGDASALDTVLNDVDVLSFSGDVHLEGVTIQGGARLKTTSGD
                     IRAELDQHDVMVDVDTRSGEATVRGPGIDIQTSRQRIPVGLAAVELSAHTGSGDIDIR
                     LT"
     gene            complement(2414..>2652)
                     /locus_tag="VN478_01680"
     CDS             complement(2414..>2652)
                     /locus_tag="VN478_01680"
                     /inference="COORDINATES: protein motif:HMM:NF021388.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2089 family protein"
                     /protein_id="HWQ21420.1"
                     /translation="LKEVQDRMDLSYPTVRNRLDKVLVSLGLAAAEKPDDKPVRRPDV
                     MELLDKLESGDMSVDAILKTIKGETDEPGQGTTE"
BASE COUNT          460 a          825 c          866 g          501 t
ORIGIN      
        1 ggcggtcttc tgcagcaggt cgtggaggta gctcatgacg tagtcgccgc cgcgctcgag
       61 gaagacgctg accggcagga ccagcgtgca ggcccgcgca gtgacgctca tgtgccagtc
      121 gaggcacacg gagaacggac agtgcttgag gaaggcaagg accttgccgg catcgggaca
      181 ttgcgaagcg ggattgccac ggacgaacat cgcggcctcg attggcggat cggcttccag
      241 cagagacgcg ccaaggttcg ggcgccatag gaagcgggga tcggcccccg gtacatccac
      301 gaggtcgtcc ggcagcaggt cgtcgtctcc ctggtcgaac cagacgccgc ctccagggat
      361 cccgtggttt cccgtcagga atccgagggc gtcgatcgta tggacgatgt ctgctcccca
      421 gcgggtgcgt tgcagaccgt agccggtcca gacgctgagc ggcggctgat ccatgacgaa
      481 gccgacgagc gcgtgcaggg cctcggcgct cagcccggtc gcctgttccg cgtcttcggc
      541 cgataccagc atgcagccct cccggaacac ctcgaaccct tcgtgaggcg ctacaggctc
      601 gccgtggagg aggatggcct cgtggcagag gtacagggcc agcgtcaggt ctgaccccgg
      661 acggatgatc caggccgagt cactggcggc ggttgtcggc gtcgcacgga catcgatggt
      721 tcccaggcgg gcgcccttgc gacgcgcgtc gtgcaggata gcgaggaaat ggacgctgtt
      781 ctccgcaggg ttgcggcccc acagcagtat ggacttgctt tcgagcgcgg cgagcggctg
      841 atcacgcccg accgtgccat agctgcgggc caggccgacc tttccctcga agccgcacag
      901 ggagccgtgt gccatcgtgc cacctcccag gaggaggggc agcaggcgct gcatccggtt
      961 gcctatgagc agattgccgg agccttcgta gaagaggaat gactgatggc catggtgctc
     1021 gaccgcagag cggatcgcag tggcggtcat gtccagggcc tcgtcccagg aaaccccgat
     1081 gaacttgccg ttctttcgta ccatggggga ggtcaggcgg tcgcggccgc gcagttcctt
     1141 gacccatcgg cgtcccttgt ggcagacgaa accgcgcgtg tactgattgt cttcccgccc
     1201 ctccagttcc atgaggcgac cttccttgca cgtgccgatc atgccgcacg agtcaggaca
     1261 gtcgtacgca cataccgtct ggtacttgcc gtcagccagc attcgttcct ccatcgctca
     1321 gagtaccgac agtatacctg agaacagtgt ggatgaaggt gaggacttgg acgaacgcga
     1381 acccccggct gcgacgaccg ggggttcgcg ttatcgtttt cggaaggaga actatgtcag
     1441 ccggatgtcg atgtcaccgc tgccggtgtg ggccgagagt tccactgccg caaggcctac
     1501 ggggatacgc tggcggcttg tctggatgtc gatcccgggc ccgcggaccg tggcttctcc
     1561 ggaccgggtg tcgacgtcga ccatgacgtc atgctggtcc agctcggcac ggatgtcgcc
     1621 gctcgtcgtc ttcaggcggg ctccaccctg gatggtcacg ccttccaggt gcacgtctcc
     1681 gctgaaactg aggacgtcaa cgtcgttgag gactgtgtcg agggcagagg cgtccccgga
     1741 gtacgacttc agcatgccgt ggactgtgca gctatccatc tcgacgtcgc ccgacttcgt
     1801 gacgacgtcg ccgtcgacga ggcattgctc caggtccagg tctcccgacg tggacgtcgc
     1861 agactgaaca tgggtgagag agcggccgtc gatgtctccg gagacgctcc ggacgttcag
     1921 tgcgccgcgg agattctcga gtgagatgtc acctgagacg gtcttgagct cgcacggcat
     1981 gttctccggg atctccagct cgacagtgtc tccgctgtcc gagccaaagt ggagagagaa
     2041 gccgtggaac tcccctccgg tcagggctga ttctatctcg acggtatcgc cgttcagacc
     2101 ggtatggaca gaagactggc cgttcaccac acggacggag gtcaggcccg ggactccccg
     2161 gacacgaagg tcctccgaga tgaggcggac catcagtctg ctgccggtcg tcccgatggc
     2221 cgactgcgag gctctcgact ggtctccagt gtcgatatgg atcgtcatcc tgggactggc
     2281 cggctgggac ggaggctgag gcaccgccgg cacttgcggt atcatcgctg ccggcacgtc
     2341 cggttccaca ctgtcaagag ccgctatcag ctcgtcatac tgctccgagg tgatgagacc
     2401 cctgtctctc aactcactca gtcgttcctt ggcctggctc atcagtctct cctttgatcg
     2461 tcttgaggat ggcgtccaca ctcatgtcgc cggactccag cttgtcgagc agctccatca
     2521 cgtcgggcct gcggacaggc ttgtcatccg gtttctcagc ggccgcgagt ccaagcgaga
     2581 cgagcacctt gtccagccgg ttgcggacgg ttggatagga caggtccata cggtcctgga
     2641 cttccttgag at
//
LOCUS       DATMZD010000081         2457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_112797,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000081 DATMZD010000000
VERSION     DATMZD010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112797"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..772)
                     /locus_tag="VN478_01685"
     CDS             complement(<1..772)
                     /locus_tag="VN478_01685"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HWQ21421.1"
                     /translation="MMTILGGPDRYIQGPGEVGRLHEHGSRLGSEFLVVADEQTMAGM
                     RDPVERAFEGTGSVLIYEEFSGICTGAEIDRLVTVADLSSCDVVLGIGGGRAINAARA
                     VAAIQRLPLMVVPTTAATGAACSSMSILTDEQGVFQELRVCPVHPAVVVVDSSVVAQA
                     PPRSLVAGMGNTVATWLEAKAHRQSDTPNLLGGKGTLSSYALAALAYDTILADGLRAL
                     VAAQNGLATRAFEHVLEANIYCSGMAFENCGCAAAHSVG"
     gene            924..1463
                     /locus_tag="VN478_01690"
     CDS             924..1463
                     /locus_tag="VN478_01690"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HWQ21422.1"
                     /translation="MARLLRSPSTALAPVPAVLVTAGTMNASDVTTLAWVGTVCSDPP
                     MISISVRPERWLHHFIVEGGAFVVNIVDTAHVRALDLCGNVSGRDTDKWGLCALTREE
                     GTATPVPLVAECPISIECVVRQTLHLGSHDMFIGEIVAVHTEERVKAGGHDLDPVCYA
                     SGQYWKLGDLIGSYGFSHK"
     gene            1668..2102
                     /locus_tag="VN478_01695"
     CDS             1668..2102
                     /locus_tag="VN478_01695"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HWQ21423.1"
                     /translation="MKRVLVAVTLLLLVMGLAGCAKKADTTSPDGDTSVEGNRTIVEV
                     DAKKVEDLIQTDRDLIIIDVSGKWAEGHIVGALDTPIDKLQETINGNLVLSASKHYVV
                     YARDEETSKKAAQMLLDSSFTVINRLKGGYDAWVANGGYTEQ"
     gene            complement(2177..>2457)
                     /locus_tag="VN478_01700"
     CDS             complement(2177..>2457)
                     /locus_tag="VN478_01700"
                     /inference="COORDINATES: protein motif:HMM:NF042524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Chromate resistance protein ChrB"
                     /protein_id="HWQ21424.1"
                     /translation="VEQCERLVYELDRETEKQKFTYAEIEENETEIVRIHKWMERIVA
                     RDWFAAPGRARAEHAIEQAEERSHLYTEQVEQRESSAVDVHLRHDPAS"
BASE COUNT          489 a          790 c          705 g          473 t
ORIGIN      
        1 tgcctacact gtgggcagca gcacatccac agttctcgaa agccatcccc gaacagtaga
       61 tgttggcctc aagcacatgc tcgaaagcac gagtggctaa gccgttctgt gccgcaacca
      121 aggcgcggag cccgtccgca aggattgtgt catatgccag cgcggccagc gcgtaggatg
      181 acagcgtgcc cttgccgccc agaaggtttg gcgtgtccga ctgtcggtgt gcctttgctt
      241 ccagccacgt ggccacggtg ttgcccatac cggccaccag cgaacgcggc ggagcctgcg
      301 caacgacgga gctgtccact acgacgactg cggggtggac cggacagacg cggagctcct
      361 ggaagacacc ctgctcatcg gtgagaatcg acatcgagct gcaagccgcc ccggtggctg
      421 cggtcgtcgg gacgaccatc aatggcagcc gctggatcgc agcgacggcg cgcgcagcgt
      481 tgattgcacg gccgcctccg atccccagga cgacgtcaca tgaactgagg tcggcaacag
      541 tgacaaggcg gtctatctcc gctccagtgc agatcccgga gaactcttca tagatgagaa
      601 ccgaccccgt tccttcgaac gcccgctcca ccgggtcccg catgccggcc atggtctgct
      661 cgtctgccac gacaagaaac tcgctgccca gccttgaccc atgctcgtga agcctgccga
      721 cctcgccagg tccctggata taccgatccg gtcctcccaa gatagtcatc atcgtcaccc
      781 cttctgccgg agcatttcta ccgctgcaag acaatgctgt ggcctgcccc tattctatgc
      841 gcccaacact tcaacgcaac catgtcgtac tatactgagc atgcgagtct ggcgctcctc
      901 tgccgactcg cgcaggagat gccatggcta gacttctcag atcaccgtcg accgcacttg
      961 cacctgtccc ggccgtgctt gtgacagcgg gcacaatgaa cgcctctgac gtcacgaccc
     1021 tcgcctgggt cggcaccgtc tgctctgatc ccccgatgat cagtattagt gtccgcccgg
     1081 aacgctggct gcaccacttc atcgtcgaag gaggtgcatt cgtcgtcaac atcgtggaca
     1141 ctgctcatgt gcgcgccctc gacctctgcg gcaacgtgtc cggcagagac acggacaagt
     1201 ggggactctg cgccctcact cgcgaagagg gcacagcgac acccgtgcct cttgtggccg
     1261 agtgccccat cagtatcgaa tgcgtcgtgc gccagaccct gcaccttggc agtcacgaca
     1321 tgttcatcgg cgaaatcgta gcggtccaca cagaagaacg tgtcaaagcg ggcggacacg
     1381 acctggatcc tgtctgttac gcgagcggac agtactggaa gctgggggac ctgatcggtt
     1441 cctatggctt ctcgcacaag tagtcgttca cattcgaccc ttccgcagac tgcttcgtga
     1501 cactgtggcg cggcaggcaa gtcaggcgtt cgttcgccgt tgcccttgca gaaatcctgc
     1561 gacactctat tcttcagcta gcatacgaga gcagaggacc cagcggcatc ggtcgcaagt
     1621 ggtcatctgg tctcgacaca tcgagttcgg gagagacgga gatgcgaatg aagagagtac
     1681 ttgttgcggt gacactcttg ttgctcgtca tgggtctggc ggggtgcgcc aagaaggctg
     1741 acacgaccag cccggacggc gatacctcag tagaaggcaa cagaacgatt gtcgaggtcg
     1801 acgccaagaa ggtcgaggac ctcatccaga ctgaccgtga cctcatcatt atcgacgtct
     1861 cgggcaagtg ggccgagggg cacatcgtcg gagccctgga cacgccgatc gacaagctgc
     1921 aggagaccat caacgggaac ctggtcctta gtgccagcaa gcactacgtc gtctatgcgc
     1981 gcgacgagga gaccagcaag aaggccgccc agatgctcct cgacagctca ttcaccgtca
     2041 tcaaccgcct gaagggcgga tatgacgcct gggtcgcaaa cggcggctac acagagcagt
     2101 agatagtaca ttcgcgaaga tgacaaagcc ccggccagat cgtggccggg gcttctctct
     2161 taccgtgtcg cctggctcag gaagctggat cgtgccgcag atggacgtcc acggcgctgc
     2221 tttcccgttg ctcgacctgc tctgtgtata ggtgtgacct ctcttccgcc tgctcaatgg
     2281 cgtgctccgc ccgtgccctt cccggcgcgg cgaaccagtc acgcgcaacg atgcgttcca
     2341 tccacttgtg gatgcgcacg atctccgttt cgttctcttc gatctcggcg taggtgaact
     2401 tctgcttctc ggtctcgcgg tcgagctcgt acaccagacg ctcgcactgt tcgacga
//
LOCUS       DATMZD010000082         3830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_103317,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000082 DATMZD010000000
VERSION     DATMZD010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3830
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103317"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1306
                     /locus_tag="VN478_01705"
     CDS             <1..1306
                     /locus_tag="VN478_01705"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HWQ21425.1"
                     /translation="TDYTSVDPSYGTVSDLHALAVACHTRGVRLIIDYELNHTSNMHQ
                     WFQSAIRSIEADPGRPRANPYLNYYNFVHGKPNSGAYTQVGTTDWYYEDEFSPSMPDL
                     NLDNPALRTQIAQFTRFWQGLGVDGFRLDAVQTYYGTTSSGDIDKNTAFVNWFTGTCR
                     SVDPDAYVVGEVWNNATTIAEYYGSDATSFFAFPFAQATGLVASTLNGSGTDASAQAF
                     CKATDVWQKMLTEYDSQPVNAPFIGNHDTARPAGFFSGDLQKTKMAAGLYLTMSGGPF
                     VYYGEELGMTGSRIDQDKRGPMYWSETEQTGMTEGPPGMDPAVHLFAAEDVQDTDSAS
                     LLNYYRRAIRLRNENPEIARGTVSVLGTMADLQICAVARIWNGSRIILVINTSKEAKV
                     IEFPRAANQYAGIRGYLSTTGAAVTLQGDRLALPPMSIVVLK"
     gene            1303..3309
                     /locus_tag="VN478_01710"
     CDS             1303..3309
                     /locus_tag="VN478_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase"
                     /protein_id="HWQ21426.1"
                     /translation="MSQLPDRVANVRAVLDILHGGKGLTGDRWFVPEHWNHAGYTGGT
                     SDPGRPGELCVDPYDFFASCIEEAIQEGGAGPELERRHLTESVIYGMLARSFTAWPHH
                     DAGEVCSGTFLKTMALLPLLRRYGVDIVYLLPVFRCSDRYRKGMIGSPYAIRDIYSVD
                     PGLHDPLLGEDTGGLLEMEFRAFVELCHWLGMKVVLDFAFRTTARDSVLIADHPDWFC
                     WIRKECAAEFRAPTVGDGSKMLALDDATLRELYTSPQTPAYLAQFSWSPDRIDAERWA
                     AVRAAAGDDLLGAIEEQFGITTVPGFSDVINDQQPPWTDATYLRFYADNAAQVSGYVM
                     DDQPPFVMQDGASLSKYPGGTPNEELWRYVEDVMPFYSTHFGIDGARIDMAHAMPIEH
                     NASMVRRIRQENPSFLLWSEELDCSKGEQAWRDGFDLISGYTYYNYKRVHEGAFNHAI
                     LDGGFLASPIPVVASVETPDTPRSALIHPDKASMRLVLALNSFMPNAVPFINSGQELL
                     ETQPMNLGLDNTEEGRFVLSADDPMAGRLAFFDPYCLHWTNADTWVDTVLEDTLRLRR
                     HYISLLSDPADFVKHDDLLHYGNLTMLCYHDSFHSGGLVVAANRSLTAELGLMLALEH
                     PAAMSVGKKVEIVYDAQGPCSRLVSSVDELVLRPCEVLVIETET"
     gene            3322..>3830
                     /gene="glgP"
                     /locus_tag="VN478_01715"
     CDS             3322..>3830
                     /gene="glgP"
                     /locus_tag="VN478_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HWQ21427.1"
                     /translation="MNERVPRVAYFCMEFGLHEKLHIYAGGLGILAGDILKAAADADR
                     PLVGVGILWRQDYTEQFIDEYDRPYDSYHEYRYDSLEDTGVVVTVPIRDEEVRVKVWK
                     CTAFGNAPLYLLDTALPGNPGWNLAGQLYGWFEEERIAQEMILGIGGVRALQALGIDV
                     DVYHFNDSHA"
BASE COUNT          758 a         1197 c         1155 g          720 t
ORIGIN      
        1 gaccgactac acgtctgtgg atccgtccta cggcaccgtg agtgatctgc atgctcttgc
       61 ggtggcatgc cacacacggg gcgtgcgcct catcatcgac tatgagctga accacacgtc
      121 caacatgcat caatggttcc agtcagcgat ccggtcgatc gaggccgacc caggcaggcc
      181 gagggcgaac ccgtatctga actactacaa cttcgtgcac ggcaagccaa actcgggcgc
      241 ctacacacag gtcggtacga cggactggta ctacgaagac gagttcagcc cttccatgcc
      301 ggacctgaac ctggacaacc ccgcccttcg gacgcagata gcccagttca cgcggttctg
      361 gcaggggctc ggcgtcgacg ggtttcgcct ggacgccgtg cagacgtact acggcactac
      421 cagctctggg gacatcgaca agaacacggc gttcgtgaac tggttcaccg gcacgtgccg
      481 ttcagtcgac ccggatgcct atgtcgtggg cgaagtgtgg aacaacgcca cgaccatcgc
      541 ggagtactac ggcagcgacg cgacgagctt ctttgcgttc ccgtttgccc aggcgaccgg
      601 tcttgtggcg tcgacattga atgggtccgg gacagatgcc tctgcccagg cgttctgcaa
      661 ggcgacggac gtctggcaga agatgctgac ggagtatgat agtcagccgg tcaatgcccc
      721 tttcatcggg aaccatgaca cggcgcgacc cgcggggttc ttctcgggcg acctccagaa
      781 gacgaagatg gccgccggcc tctacctcac catgagtggg ggaccgttcg tctactatgg
      841 cgaggagctt ggcatgaccg gctcgcgcat agaccaggac aagcgcggcc cgatgtactg
      901 gtccgaaacg gagcaaaccg gcatgaccga ggggcctccg gggatggacc ccgccgtcca
      961 tctgtttgcc gccgaggacg ttcaggacac agattctgcc tcgctgctca actactaccg
     1021 ccgcgccatt cgcctgcgga acgagaatcc ggagatcgcc cgtggcaccg tgtccgttct
     1081 tggcacgatg gctgacctcc agatctgtgc cgtcgccagg atatggaacg ggtcacgcat
     1141 catccttgtg atcaacacgt caaaggaggc caaggtgatc gaattcccca gggcggcaaa
     1201 ccagtacgcc ggcatccgcg ggtacctgtc gacgacgggc gctgcagtga ccctgcaggg
     1261 agatcggctc gccctgccgc ccatgtccat cgtggtgctg aaatgagtca gctgcctgat
     1321 cgtgtcgcaa acgtcagggc cgtgctcgac atcctgcacg gcgggaaggg cctgacgggt
     1381 gacaggtggt tcgtccctga gcactggaac catgccggct acaccggcgg aacatcggac
     1441 cctggcagac ctggcgaact atgcgtggac ccgtacgatt tctttgcctc ctgcatcgaa
     1501 gaggccatac aggaaggcgg tgccggacca gagctggaga gacgccacct caccgagagc
     1561 gtcatctacg gcatgctggc gcgttccttc accgcctggc cacatcacga cgccggcgag
     1621 gtctgctcgg gcacgttcct gaagacaatg gccctgcttc cgctcctgcg gcggtacggg
     1681 gtcgacatcg tctacctcct gcccgtgttc aggtgcagtg accggtacag gaagggcatg
     1741 atcggcagcc catatgcgat cagggatatc tacagcgtcg accctggcct ccacgacccc
     1801 ctcctgggtg aagacacagg cggactcctg gagatggaat tccgtgcctt tgtcgaacta
     1861 tgccactggc tgggcatgaa ggtggtcctg gacttcgcct tccggacgac cgcacgagac
     1921 agcgtactga tcgcggatca tccggactgg ttctgctgga tccggaagga gtgtgctgcc
     1981 gaattccggg ctcccacggt gggtgacggc tcgaagatgc tagctctgga cgatgcgacg
     2041 ctgagagagc tctacacgtc gccacagacg cctgcgtacc tcgcccagtt ctcgtggtct
     2101 cctgaccgca tcgacgcaga acgctgggca gcggtgcggg ccgctgcggg cgacgacctc
     2161 ctcggcgcga tcgaagaaca attcggcatt acgactgtac ccggtttctc agacgtcatc
     2221 aatgatcagc agccaccatg gacggatgcc acctacctgc ggttctacgc cgacaatgct
     2281 gctcaggtca gcgggtacgt catggacgac cagcctccgt tcgtcatgca ggacggcgct
     2341 tcgctcagca agtatcccgg cggaacgccc aatgaagagc tctggcgcta cgtcgaggac
     2401 gtcatgccat tctacagcac ccacttcggc atcgacgggg cccgtatcga catggcgcat
     2461 gcgatgccga tcgaacacaa tgcgtcgatg gtgcgccgca tccggcagga gaacccctca
     2521 tttctcctct ggtcggagga gctcgattgc agcaagggcg aacaggcgtg gcgcgacggg
     2581 ttcgatctca tcagcggcta tacctactac aactacaaga gagtccacga gggcgccttc
     2641 aatcatgcca tcctggatgg tgggttcctc gcctcgccga ttcccgtggt cgcctcggtg
     2701 gagacgcccg acactccccg atcggcactc atccaccccg acaaggcctc catgcgactc
     2761 gtcctcgcgc tcaacagctt catgcccaac gctgttccgt tcatcaatag tgggcaggag
     2821 ctactggaga cgcagccgat gaaccttggg ttggacaaca ccgaagaggg tcgcttcgtc
     2881 ctttcagccg atgacccgat ggccgggagg ctcgccttct tcgaccccta ctgtcttcac
     2941 tggacaaacg ccgacacgtg ggtggacacc gtccttgagg acacgctgcg cttgcggcgg
     3001 cactacatct ctcttctcag cgatcccgca gacttcgtga agcacgacga ccttttgcac
     3061 tatgggaact tgacgatgct ctgctatcat gactcgtttc attccggtgg actcgtcgtc
     3121 gcagcgaatc gatcgctgac ggcggaactg ggcttaatgc ttgcgttgga gcaccctgca
     3181 gcgatgtctg tcggaaaaaa ggtcgaaatc gtgtatgatg cacagggccc ttgcagtcga
     3241 ctggtctctt cagtcgacga actcgtgctc cggccgtgtg aagttctcgt catcgaaact
     3301 gaaacatagc ggaggcttgt aatgaacgaa cgagtgccca gagtagccta cttctgcatg
     3361 gagttcgggc tgcatgagaa gcttcacata tacgccgggg gcctgggcat cctggcaggc
     3421 gacatcctga aggcggcagc ggacgctgat cgacccctgg tcggggtggg catcctgtgg
     3481 cgccaggact acaccgagca gttcatcgac gagtacgatc gtccctacga cagctatcac
     3541 gaataccgat acgactccct ggaggacacg ggcgtggtcg tcaccgttcc tatccgcgac
     3601 gaggaagtgc gggtgaaggt gtggaagtgc accgcattcg gcaacgcacc gctgtatctg
     3661 ctggacacgg cccttccggg caaccccgga tggaacctcg cggggcagct ctacggctgg
     3721 ttcgaggaag aacgcatcgc ccaggagatg atcctcggca tcggcggcgt gcgtgccctg
     3781 caggcgctgg gcatcgacgt ggacgtctat cacttcaacg acagccacgc
//
LOCUS       DATMZD010000083         2704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_112821,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000083 DATMZD010000000
VERSION     DATMZD010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112821"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VN478_01720"
     CDS             complement(<1..296)
                     /locus_tag="VN478_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011943591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21428.1"
                     /translation="MRELADLFDRPPAGQSILFAGPSGSGKTEVAINLALSLVRQTTS
                     PVTLCDLDVVKPYFRLRDMLRLLTEDEASRLRIVEPERRLLHADIPTFPRHLHAL"
     gene            complement(318..1418)
                     /gene="buk"
                     /locus_tag="VN478_01725"
     CDS             complement(318..1418)
                     /gene="buk"
                     /locus_tag="VN478_01725"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HWQ21429.1"
                     /translation="MQFRILVVNPGSTSTKLSIFEDQTPVVTETVSHDAETIAQFGSL
                     MDQLPMRLKIVKDFLGQHGTSLADLSAVACRGGLLRPLPSGTYRVDEEMVRDLHQPLL
                     GAHASNLAACIGYELSRESGVPAFIVDPVVVDELTPLARLSGLKGIDRVSIFHALNQK
                     AVARQAATDIDKPYEEANLIVAHLGGGISVGAHHLGRVVDVNNALNGDGPFAPERAGS
                     LPAAGLVRLCFSGAYSQSEVMRLLAGKGGLVSHLGTNDARTVESMIASGDGHALLVYT
                     AMAYTVAKTIGEMATVLRGDVDAIVITGGMARSPLLVDEITKRVEFIAPVKVYPGEDE
                     MKALALGVLRVLDGSAPAGVYPPAETATYSRP"
     gene            complement(1498..>2704)
                     /locus_tag="VN478_01730"
     CDS             complement(1498..>2704)
                     /locus_tag="VN478_01730"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HWQ21430.1"
                     /translation="TKLGQNPEITEILRNPQRTLCVSIPVRMDDGTTRVFQGFRCQHN
                     DAVGPTKGGIRFHQDETLEDVKALATLMTLKCAVVGLPYGGGKGGIVVDPTKLSTGEL
                     ERLSRGFIRALGTFVGPDKDVPAPDVNTNGQIMAWMIDEYSKLTGHNTPALMTGKPLI
                     LGGSLGRTAATGLGGVYCLRMAAKKLGMNLKGATVAVEGFGNVGFYTAKILHDEDGAK
                     IVAITMVNGGVYDPKGIDPDDLKAFETKNGTVKGYKGLDNVSNEQLRALAVDVYVPAA
                     LQNAIFKDNAKDVKAKIVVELANGGINPDADEILEKNGAFVVPDILGNAGGVTVSYFE
                     WVQNNYGYYWSEKEVNEKLDRVMTEAFERVYGMYTKHKTDKVTMRMAADMVAVERVGE
                     AVKIRGWVG"
BASE COUNT          565 a          828 c          738 g          573 t
ORIGIN      
        1 agagcatgca gatgcctggg aaacgtggga atgtctgcat gaaggagccg acgttccggc
       61 tcaacgattc tcagtctgct tgcctcgtcc tcagtgagca gccgcagcat atcacgaagg
      121 cggaagtacg gcttgacgac atccaggtca cagagagtga ccggcgatgt cgtctgacga
      181 accagcgaca gggccagatt gatggcaact tctgtcttgc ccgatcccga gggtcccgca
      241 aacaggatcg actgacccgc cgggggtcgg tcaaacagat cggcaagttc acgcatctgt
      301 cagagcgtcc gacgtaatca gggccgtgag tatgtcgctg tttcggcagg cgggtacaca
      361 cccgcgggcg ccgaaccgtc caacacgcga agtactccca gcgccagcgc tttcatctcg
      421 tcctcgcctg ggtagacctt gaccggtgcg atgaactcta cccgctttgt gatctcgtcg
      481 acaaggagtg gtgaccgcgc catgcctcca gtgatgacga tcgcatcgac gtcccccctc
      541 aataccgtag ccatctcccc gatcgtcttg gccaccgtgt atgccattgc ggtgtacacc
      601 aggagcgcat gcccgtcacc tgaggcgatc atgctctcaa ccgtacgagc gtcattcgtc
      661 cctagatgac tcaccaaccc tcccttgcct gcaaggagtc tcatcacttc ggactgcgaa
      721 tatgcacctg agaagcacag tctcacgagt ccagcagccg gcaggctgcc tgctctctcg
      781 ggagcgaacg gtccatcgcc attgagggca ttgttgacgt caacgacccg gcccaagtgg
      841 tgcgctccga cagatatccc tccgccgagg tgagccacga tgagattggc ctcttcatag
      901 ggcttgtcga tgtccgtggc tgcctgtctg gcgacagctt tctggttgag agcgtggaag
      961 atgctcacac gatcaattcc cttcaagccg gagaggcgag ccagcggagt cagttcgtct
     1021 acgacgacag gatccacgat gaacgctgga acacccgatt ccctggatag ctcgtatcca
     1081 atacacgccg ccagattgga cgcgtgagct cccagcagag gttggtgcag gtcacgaacc
     1141 atttcctcgt cgacacgata tgtccctgag ggaagtggtc gcagcaaacc tcccctgcag
     1201 gcaactgctg acaggtcagc caggctcgtg ccgtgctgcc cgaggaaatc cttgacaatc
     1261 ttcaggcgca tgggcagttg gtccatgagc gatccaaatt gggcaatggt ctcggcatcg
     1321 tgggatacgg tttcggtcac gacaggggtc tgatcctcga aaatagacaa tttggtgcta
     1381 gtggagcctg gattgacgac aagaatcctg aactgcatgg ccgcctccaa cagactagat
     1441 gcaccagcaa gggggagggt gctgcacgca ccctcccccc tagacagtcg ctacttgtca
     1501 gcccacccag ccacgaatct tgacggcctc accgacacgc tcgactgcga ccatgtcagc
     1561 agccatgcgc atagtgacct tgtccgtctt gtgcttcgtg tacatgccgt agacgcgctc
     1621 aaatgcctcg gtcatgacgc gatcaagctt ctcattgact tccttctccg accagtagta
     1681 gccatagttg ttctggaccc actcgaagta ggagacagta acgcctcctg cattgcccag
     1741 gatgtccggg acgacaaagg ccccgttctt ctcaaggatc tcatctgcgt caggattgat
     1801 gccaccgttt gccaactcca cgacaatctt cgccttgacg tccttcgcat tgtctttgaa
     1861 gatggcgttc tgaagagccg ctggaacgta gacatcaaca gccagcgcac gcagctgctc
     1921 gttggagacg ttgtcaagtc ccttgtaacc cttcacggtt ccgttcttgg tctcaaaagc
     1981 cttcagatcg tcagggtcaa tgcccttggg atcgtagact ccaccgttga ccatggtgat
     2041 ggccacgatc ttcgcaccgt cttcgtcatg caggatcttg gcggtataga agcccacgtt
     2101 tccaaatcct tcgactgcaa ccgttgcgcc tttcaggttc attccaagct tcttggctgc
     2161 catccgcagg cagtagactc caccaagccc ggtcgctgca gttctcccaa gagatccgcc
     2221 caggatgaga ggtttgcctg tcataagagc tggggtgttg tgccccgtca gcttgctgta
     2281 ctcatcaatc atccatgcca tgatctgccc gttggtgttc acgtcaggag ctggaacatc
     2341 cttgtcgggg ccaacaaacg tacccagagc gcggatgaag ccgcgagaca gccgctcaag
     2401 ttcgccggtc gacaacttgg tcgggtcgac cacaataccg cccttgccac cgccgtacgg
     2461 aaggcccacc acagcgcact tgagcgtcat gagggtagcc agggccttca catcctcgag
     2521 ggtctcatcc tgatggaacc ggatgccacc cttagttgga ccgacggcat cgttgtgctg
     2581 gcaccggaat ccctgaaaga ctctcgtcgt accatcgtcc atgcggacag gaatcgagac
     2641 acagagagtc ctctgcgggt tgcggagaat ctccgtgatc tcggggttct ggccgagctt
     2701 cgtg
//
LOCUS       DATMZD010000084         4724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_62648,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000084 DATMZD010000000
VERSION     DATMZD010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4724
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62648"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1244)
                     /locus_tag="VN478_01735"
     CDS             complement(<1..1244)
                     /locus_tag="VN478_01735"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HWQ21431.1"
                     /translation="MAPAHKVGIMETVLRDAQQSLIATRMTTKEMEPAFEAIDAVGYR
                     SIEMWGGATFDSCLRFLHEDPWERLRTIRRAMKHTKLQMLLRGQNVLGYKNYADDVVD
                     AFVERSLANGIDIIRVFDALNDMRNVERAIRTTKRLGGNAQGCIVFTIAPTYTVDKYV
                     GIARELEQMGVDSIAIKDMAGLLTPYATYDLVSALKKMTNLPIQIHSHYTTGLAGQSY
                     LKGIEAGADIVDCAISPFAMATSQPCTETMVAALKGTPYDTGIDLEALNAVAQFFMPV
                     REKALESGVLDAKVMGVDVNALIYQLPGGMISNMVSQLKQANMLDRYQEVLKEVPQVR
                     ADLGYPPLVTPTSQIVGTQAVFNVMMGRYVQCTKETKALVKGEYGRASVPISDEIKQL
                     IIGDEPTIDCRPADLIEPQMAR"
     gene            complement(1575..2741)
                     /locus_tag="VN478_01740"
     CDS             complement(1575..2741)
                     /locus_tag="VN478_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21432.1"
                     /translation="MRKSLGKVVLAVVIAGLLTGGIAIASAATRVRTVTTPQGTTVDV
                     ISVTGTVTEVSTPTTAESGKGFMGMFRHGSEGSAKIKDSADGTVYTLEFGREEAAGIT
                     MKVGDTVTVEGTSRTRNSVNMLQIWTFTGADGKTVTLRKADGTPNIETVSVAGTVTEV
                     NVPVTAPSTGDTAKPAPKAMATIKVKQADGTIMTVVLGRDSSSITVKVGDAVKIEGFK
                     TPMDAATIMATSFTGADGKTVTLRGLDGMDGGRGIQGESVTISGTVTEVTQATATTPT
                     PNDATRPVPQAMMTIKVKQADSTVLTVLIGHDSSSITVKVGDAVKIEGFKTSTDATTI
                     MATSFTGADGKTVTLGGKGFGGCGMRGGPGDGRGFGGGMKRPGSVSDSTDPSNT"
     gene            complement(2949..3749)
                     /locus_tag="VN478_01745"
     CDS             complement(2949..3749)
                     /locus_tag="VN478_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21433.1"
                     /translation="MSRYDAMVIIVTMSAFAVFTVLGIWSLVRREKARAIWFSVFALM
                     LGTLGSFILMAGVIMFILPGNQSPFLIIALSLPIMAGFVLLLYKTKVVGMRAKLTIVV
                     VVLLLGLVAGRSTAVSAHAYQAVRNAFFPLLQKEYAVKGTVVNAEPCFVLYDLDAKEF
                     NFAKSGLFTHYSTDVDKINAIVFYSKNTRLVGEWVTETGHNRVADAHSEFIKVTVVDV
                     KTWSVVASQSFEGVGTPDKEKGDYTNRISITDNQIQGYLNNLFSTTDG"
     gene            complement(4291..>4724)
                     /locus_tag="VN478_01750"
     CDS             complement(4291..>4724)
                     /locus_tag="VN478_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21434.1"
                     /translation="YKVGTLAKTEQKGSSWRDSERLLQVRQGLPVFLTFCIILGLVLP
                     IFFGQVKRVTAPEGETVVHIQATYKGLPWSGRLAFRLNGEQQWTGSMVPYEIFVHPGV
                     YTVTVAGGGPGESRLLDVTPAGSQLGRVGETVTFTAEFASP"
BASE COUNT          905 a         1449 c         1333 g         1037 t
ORIGIN      
        1 aacctggcca tctgaggctc gatcaggtcg gcagggcggc agtcgatggt tggctcgtcg
       61 ccgatgatca gttgcttgat ctcatcggag atgggcaccg aggcgcggcc gtactcgccc
      121 ttgaccagtg ccttggtctc cttcgtacac tggacgtagc gacccatcat cacgttgaag
      181 acggcctgtg tcccgacgat ctgagacgtt ggggtcacga ggggcggata accaagatca
      241 gcgcgtacct gtggcacttc cttgaggacc tcctgatacc ggtcaagcat gttggcctgt
      301 ttgagctggc tgaccatgtt tgagatcatg cctccgggca gctggtagat gagcgcgttc
      361 acgtcgactc ccatgacctt ggcatccagc acacctgatt ccagtgcctt ctcgcgcacc
      421 ggcatgaaga actgtgccac agcgttcaat gcttcgaggt cgatgcccgt gtcatacggt
      481 gtacccttga gcgctgctac catggtctcc gtgcagggct gggatgttgc catcgcgaag
      541 ggactgatgg cgcagtcgac gatgtcggcc cccgcctcaa tgcccttcag gtacgactgt
      601 cccgccagtc cggtcgtgta gtggctgtga atctggatag gcaggttcgt catcttcttg
      661 agggccgaca ccaggtcata ggtcgcgtat ggagtgagca ggcccgccat gtccttgatg
      721 gcaatgctgt cgacgcccat ctgttcaagt tcgcgggcaa tgcctacgta tttgtcgacg
      781 gtgtaggtcg gggcaatagt gaagacgatg cagccctgcg cgtttcctcc aagtctcttg
      841 gtcgtgcgga tggcccgctc tacgttgcgc atgtcgttga gagcgtcgaa aacgcggatg
      901 atgtcgattc cgttggccag cgatcgctcg acgaaagcgt ccacgacatc gtcggcgtag
      961 ttcttgtacc cgagtacgtt ctgaccgcgc agcagcatct gcagcttcgt atgcttcatg
     1021 gcacggcgga tcgttcgaag gcgttcccat gggtcttcat gcaggaagcg caggcaggag
     1081 tcgaaggtcg ccccgcccca catctcgatg gagcgatatc cgaccgcatc gatcgcctcg
     1141 aacgcgggct ccatttcctt cgtggtcatg cgggtcgcaa tcagcgactg ctgcgcatcc
     1201 ctgaggacag tctccatgat gccgaccttg tgagccggag ccatcgcaga ggatgaggac
     1261 gccaagtcag gtctgcttcc tgcaccgatc tcctcgatct cgacgtcgaa cgacttgccg
     1321 ccgacattca tgctgaactt gtttgccacg tgaaccccct ctgcagcgat ttctcagcgc
     1381 cgaacccgtc agaacagcca caccctctgc cgtgtgcgcg ctgtcattat accctattgt
     1441 gtcctaatgc ggcacaccct ttgcgacctt gcgtcgctac gccaccactc ccctggaaac
     1501 ggccaggaag agcctgaata tgagtgaccc ccgccgaagc gggggtcact ccaaacgatc
     1561 agacgagtag agtgctacgt gttggacgga tcagtggagt ctgagacaga tcctgggcgc
     1621 ttcatgccgc caccgaaccc acgaccgtct ccggggccac cgcgcatgcc gcagcctccg
     1681 aatcccttgc cgccgagggt cacggtcttg ccgtctgccc cggtgaacga ggtagccatg
     1741 atcgtggtcg cgtcagtcga agtcttgaaa ccttcgatct tgactgcatc gccgaccttc
     1801 acagtgatgc tgctcgagtc atgtccgatg agcactgtga gcacggtgct gtcggcctgt
     1861 ttgaccttga tcgtcatcat ggcctgtgga acgggcctgg tggcgtcatt aggagtggga
     1921 gtcgtcgcgg tcgcctgagt tacctcggtc accgtacccg aaatggtcac gctctcgccc
     1981 tgtattccac ggccgccgtc catgccgtcc aggccgcgaa gggtaaccgt cttgccgtct
     2041 gcgccggtga acgaggtagc catgatcgtg gctgcatcca tcggagtctt gaagccttcg
     2101 atcttgacgg catcgccgac cttcacagtg atgctgctcg aatcacgtcc gaggaccacc
     2161 gtcatgatgg tgccgtcggc ctgtttgacc ttgatcgttg ccattgcctt cggggccggc
     2221 ttcgccgtgt cgccggtcga gggagccgtc actggcacgt tgacttcagt aacggttccc
     2281 gcgacgctta ccgtctcgat gttgggcgtc ccgtcagcct tgcgcagagt cacggtcttg
     2341 ccgtctgctc cggtgaacgt ccagatctgc agcatattga cgctgttgcg agtcctggac
     2401 gtcccttcga cggtgacagt gtcaccgacc ttcatcgtta tacccgctgc ttcctcgcgg
     2461 ccgaactcga gtgtatagac cgtcccgtca gcactatcct tgatcttcgc gctgccctcg
     2521 gacccgtgcc tgaacatccc catgaaaccc ttgccggatt ccgcggtcgt cggagtcgag
     2581 acctcggtaa cggtgcctgt caccgaaatg acatcaacag tggttccctg cggcgttgtc
     2641 acggtccgta ctctggtagc tgctgaggct atggcgatac cgcctgtaag gagtccggcg
     2701 atgacgaccg ccaggacaac cttccccaat gatttcctca tgtggtctcc tctgttacgg
     2761 ggtcaccccc ggagtgcggc acccatggat gctgccacct ctagtacaag acagcgaagg
     2821 gtttcgtacc gggcatcctg tgaagaacgt tgtcctcggc tcccgcgaaa ccgcgttcag
     2881 gtcaggccgg cgtccggtgc ccacgacttt ctccggcatg gggatcacgc tccaccgcac
     2941 ccatacggct atccgtctgt tgtggaaaac aggttgttga gatagccctg gatctggttg
     3001 tcggtaatgg agatcctgtt tgtatagtcc cctttctcct tgtccggcgt tccgactccc
     3061 tcaaagcttt ggctggcgac gacagaccat gttttgacgt ccacgacagt caccttgata
     3121 aattcggagt gggcatccgc gactcggttg tgtccagttt ctgtgaccca ttcgccgaca
     3181 agcctcgtgt tcttgctata aaaaacgatc gcgttgatct tgtccacatc tgtgctgtaa
     3241 tgagtaaaca ggccgctctt ggcgaagttg aattccttgg cgtcaaggtc ataaagaaca
     3301 aaacacggct ctgcatttac gacagtgcct ttgactgcgt attccttctg gagcagcggg
     3361 aagaatgcgt tacgcacggc ttgatacgca tgcgcactga ctgcggtaga acggccggcg
     3421 accaagccca gcagcaaaac gacaaccaca atggtcagct tggccctcat gccgacaacc
     3481 ttcgttttgt acagcagcag aacgaagccg gccatgattg gcagggacag ggcaatgatc
     3541 aggaacgggc tctggtttcc gggaaggatg aacatgatca cgccggccat cagaatgaaa
     3601 cttccaaggg tccccagcat cagcgcaaac acggaaaacc agatggctct tgccttctcc
     3661 cttctcacca ggctccagat acccaggacc gtgaacactg caaaagcact cattgtgaca
     3721 atgatgacca tcgcatcata tctcgacatc cccgtcctcc tcggttctgc tactcttgta
     3781 gttggtgaac ctgccgacct gctatatgga tgccgccgcc gatgccactc acagcgatcc
     3841 tgcgctgatt tcgcctgggt tcacgtttcc acttgcggta acgtcaggag cgcagtagtc
     3901 ctcagacgtg aacccagagt cacccatgga cttcttcgac aacgcctggc tcccatctcg
     3961 ctgtcggcgt caagcactgc ccatggtaag catagcagac atggcatgac ggacaagaaa
     4021 gacggggcga gacgtgtgcc ttcgatcgtt gctaccgccc aggtgtcaag gtcgttgaga
     4081 tagatgctat caagtctctc ctctcggccg acagacctga ttgcctccct tgttcccatc
     4141 tacacggatg ctgacgtgac aaccagcaag cgccgaccat cgctcgtgag gttggtcatg
     4201 cggcgtccgg atgccatctg gggaaccaag cgacaatacg ggatcgggct cctccctgtg
     4261 gggcaacgat gatggcgaag ttgctggcgt ctatgggctg gcaaactccg cggtgaacgt
     4321 gacagtctcc ccgacacgac ccagttgcga acccgccggc gtgacgtcaa gcagcctgga
     4381 ctctccgggt ccaccaccgg cgaccgtcac cgtataaacg cccgggtgga cgaagatctc
     4441 gtacgggacc atcgagccgg tccattgttg ctctccgttc agccggaatg ccaggcgacc
     4501 cgaccagggc agtcccttgt aagtggcctg gatgtggacg acagtctcac cttcgggggc
     4561 ggtcactcgc ttcacctgcc cgaagaagat gggcaggacc aaccccagga tgatgcagaa
     4621 ggtcaggaag accggcaagc cctgcctcac ctgcaacagt ctctcggagt cgcgccagct
     4681 ggaacccttc tgttcggtct tggccaaagt tcccaccttg tacc
//
LOCUS       DATMZD010000085         5113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_123103,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000085 DATMZD010000000
VERSION     DATMZD010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5113
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123103"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VN478_01755"
     CDS             complement(<1..249)
                     /locus_tag="VN478_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21435.1"
                     /translation="MRRSSTKAAPDILAAPGSSLGLDMVQEIRRASRGLAFALVGYIA
                     VFIVQLTSYFITHMLSILAQSFATLANAVVALVVLLALA"
     gene            437..841
                     /locus_tag="VN478_01760"
     CDS             437..841
                     /locus_tag="VN478_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21436.1"
                     /translation="MDPLWVLVILVGGSSLLVAIVFVFIAVKVAGVARQVGPRVTEMQ
                     ERIAAAGIEIARVSEAVVSAEEQFNGLVDKASTASRRVTRVVSVVPRAIHVVQDVRIK
                     AEATVRAVGVVAARTIYNARERSSSSGEVTLK"
     gene            1049..2530
                     /locus_tag="VN478_01765"
     CDS             1049..2530
                     /locus_tag="VN478_01765"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HWQ21437.1"
                     /translation="MDTTSLIRADELEQILLETTARKAVQRAVDLIAESIPSCSVTVL
                     GVDADRSVHFDAANGIPWSVLRKVEVSFNRELPPNVLGIIRDQEICFIQDVGEYPGWR
                     RRSASDINSYVGFPVIVDRRVVSIINVQTSAQKLKPEDVDALRPFIHLISLIIGRYLK
                     EQQSANRENFLSLLHETTLDGIRATSPQEFMDTVVQRIARRLGYQHIAILLYDDEADT
                     LVLRAQRGYTSDYDGLRLPVRDRAGVVVRAFRLRRAVNVPDAASSSFYLRGVPGGRSD
                     LALPLMVGEKVIGVLNLESKKVAAFSREDIRNLTPLAAGIGLMLATMEIKQLLRQQAM
                     LDGLTGAHNRRAMDEIVTEELERARRHGHDLSFVMMDVDDFKSINDQLGHAEGDRILE
                     MLVAVLRKSLRAMDKVIRYGGDEFLLVLPETSQRETELLLERLHACVETQVVTTLGGI
                     SCSAGVATVRGDPDGGELVELADKRMYQAKARYRARRQEDPGY"
     gene            2575..3138
                     /locus_tag="VN478_01770"
     CDS             2575..3138
                     /locus_tag="VN478_01770"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HWQ21438.1"
                     /translation="MDILPRVIDGKHVFLAPERRDNIPLYWKWANDREVTRLTQGYGR
                     APSLELMEASYEKSAVADDVRRFGIHLLSTGDIIGRCALKNLNLIVGRGEASILIGDE
                     KYRGLGYGTEAMRLLCVVGFDMLELHTIFLTVLACNPAADASYRKVGFRDAGCLREAS
                     ARDGKRVDILYMDLLRGELTPDPESGA"
     gene            complement(3160..4989)
                     /locus_tag="VN478_01775"
     CDS             complement(3160..4989)
                     /locus_tag="VN478_01775"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HWQ21439.1"
                     /translation="METSILTKIAARVRYDILHMSTQAGSGHPTTSLSAADLMTGLFF
                     GGTFHFDLDEPQNPCNDRVIFSKGHASPLFYALYAAAGVLSQEDLLTYRKMGSPLEGH
                     PTPRFAYAEAATGSLGQGLGIGVGIAMNGRFVDKLPYNTYVLMGDSEVAEGSVWEAAE
                     LASHYGLDNLVGILDVNRLGQRGETMLGWGLDTYEARFAAFGWHTIVLEDGHDMDAVQ
                     AAYGEIDHACGRPTVLIAMTIKGKGVSFLENKDGWHGKTLKGEEFDRAVLELGPIDLN
                     TLGTIEKPVRATPAMWAPVRVDQSSDSRVVSPREAYGRALVDIAPTHADMVVLDAEVS
                     NSTFAATFRNTYPERFFEMYIAEQNMVSAALGFSTRGRIPFVSTFAAFFSRAFDQIRM
                     AQYAMDRANIKFVGSHVGVAIGPDGPSQMGLEDMAMFRTLVDSVVLYPADDTSTAALV
                     RQMADHHGICYMRTTRQEMPRLYETGEEFPIGGSKTLRHADDDHVTLVSAGITLHEAL
                     KAADTLAAEGIHARVIDLYSVKPLDLTTLHRATAETSAIITVEDHYAEGGIGEAVASA
                     LSDAATPVFSLAVRKLPMSGKPEELLAYEGIDAAAIVRRVREL"
BASE COUNT          966 a         1513 c         1660 g          974 t
ORIGIN      
        1 ggcgagggca agcaggacga cgagggcgac cacggcgttg gcaagggtcg cgaacgactg
       61 cgccaggatg gacagcatgt gcgtgatgaa gtagctggtc aactggacga tgaagacagc
      121 aatgtagccc acgagcgcaa acgcgagccc gcgacttgcg cgccgaatct cctgcaccat
      181 gtccagtccg agcgatgatc ccggggctgc aagtatgtct ggtgctgcct tggtgctgct
      241 ccgtctcaca gtgtcgtgct ccttgcgaaa ggtcgccttt tcttgctgca cacatggtgc
      301 cacactgaat cgatattgca agagcgacaa cgagagtgca tgcgcgagtc acatgcgtgc
      361 gcggcggcgg ctgtgacatg aatcgtatcc tgcgctatac tgccagcagt actggttcac
      421 gccgaggagg tagcacatgg acccgttgtg ggtactcgtc atattggttg gcggttcgtc
      481 gctgcttgtt gcgatagtct ttgtgttcat agctgtcaag gtggcgggtg tggcacgcca
      541 ggtcggcccc agggtgaccg agatgcagga gcggattgct gccgctggta tcgagattgc
      601 gagagtttca gaggctgtcg tttcggctga ggagcagttc aacggtctgg tggacaaggc
      661 gtcgacggca tcgcgccgcg tgacaagagt cgtcagtgtc gtgccgcgcg ccatccatgt
      721 cgtccaggac gtcaggatca aggcggaggc gaccgtgcgc gccgtggggg tcgttgccgc
      781 caggaccatc tacaatgccc gtgagcgctc ctcgtcttcc ggggaagtca cactcaagta
      841 gacacccggc gtcgctccac gactcagcgg tttctccggc agtgcatgga tcaggcagtg
      901 tcactctctt cccgtggtgt ctcgggactc ggaagtggcg acggagtccc agaaacgtgc
      961 gtccgaagcc acagtccgaa ccgtgtgtcg ccgctcgcag cggggcagag ttgcagccga
     1021 atcgcagggg tctacaataa gtccatccat ggatacgacg tcgctcattc gtgctgatga
     1081 actggaacag atcctgctgg agacgacggc tcgaaaagcc gttcagcggg ctgttgatct
     1141 catagccgag tcgatcccct cgtgctcggt gaccgtactg ggcgtagatg ctgaccggag
     1201 tgtacacttc gacgcggcca acggcatccc gtggagcgtg ctccggaagg tcgaggtcag
     1261 cttcaaccgg gaactgccgc cgaatgtcct gggcatcatc cgcgatcagg agatatgctt
     1321 catccaggac gttggcgagt atccagggtg gcggaggagg tcggccagcg acatcaatag
     1381 ctatgtcggt tttccggtca tcgtagacag gcgtgtagtc agcatcatca acgtgcagac
     1441 gtctgctcag aagctgaaac cggaggacgt ggacgcgctg cggcccttca tccacctcat
     1501 cagtctgatc atcggccggt atctgaagga acagcagtcc gcgaacaggg agaacttcct
     1561 gtccctgctg cacgagacga cactggacgg tattcgcgcc acaagcccgc aggaattcat
     1621 ggacacggtg gttcagcgca ttgcgcggcg cctcggctac cagcatatcg cgattctcct
     1681 gtatgatgac gaggccgaca ctcttgtcct gcgggcgcag cggggataca cgagcgacta
     1741 tgacggtctc agattgcctg tccgcgaccg ggcgggcgtg gtcgtacggg cctttcgcct
     1801 gcggcgtgcc gtcaacgtcc ccgatgccgc atcctcttcc ttctacctgc gcggcgtgcc
     1861 gggagggcgc tctgatctgg ccctgccatt gatggtggga gagaaggtca tcggagtcct
     1921 gaacctggaa agcaagaagg tggctgcatt ctcacgtgag gacatccgca acctgacgcc
     1981 gcttgcggct ggtatcgggc tcatgctggc gacgatggag atcaagcagc ttcttcgcca
     2041 gcaggccatg ctggacggtc tcacgggggc acacaaccgt cgtgccatgg atgagatcgt
     2101 caccgaggag ctggaacgtg cccgacggca cggccacgac ctctcgtttg tgatgatgga
     2161 cgtcgacgac ttcaagtcca tcaacgatca gcttggccat gccgagggag accggatcct
     2221 cgagatgctg gttgcagtgc tgcggaagtc cctgcgcgcg atggacaagg tcatccgcta
     2281 cggcggggac gagttcctcc ttgtgcttcc cgagacatca cagcgggaga cggaactgct
     2341 gctcgaacgg ctccacgcgt gcgtcgaaac gcaggttgtg acgacactgg ggggtatcag
     2401 ctgttctgcc ggggttgcca cggtgagggg agatcccgat ggcggagaac tcgtagagct
     2461 tgcagacaag cgcatgtacc aggccaaggc caggtaccgc gcgcgacgtc aggaagaccc
     2521 tggctactaa ctgagaccat acgacgagaa agacaagaga ggagagggac catcatggac
     2581 atcctgccgc gcgtgatcga tgggaagcac gtgtttctgg cgcctgaacg gcgcgacaac
     2641 atcccgctct actggaaatg ggccaacgac cgtgaggtga cccgcctgac ccagggatat
     2701 ggcagagccc ccagccttga gctgatggag gcctcttacg agaagtccgc ggtggcggac
     2761 gacgtccgac gattcggcat ccacttgttg tcgacggggg acatcatcgg acggtgcgcg
     2821 ctcaagaacc tcaacctcat cgtggggaga ggtgaagcga gcatcctcat cggagacgag
     2881 aagtaccgag gcctggggta cggtaccgag gcaatgcggc ttctatgtgt agtcgggttc
     2941 gacatgctgg aattgcacac gatcttcctg acggtgcttg cctgcaaccc cgctgccgac
     3001 gcatcgtacc ggaaagtggg cttcagggac gcaggatgcc tgcgcgaggc gagcgcccgc
     3061 gacgggaagc gtgtcgacat cctctacatg gacctgctgc gcggtgagct gacgccggac
     3121 cccgaatcag gagcctgacg ccagaagggc cgacactcgc tacagctccc gcactctccg
     3181 gacgatggcc gcagcgtcga tgccttcgta ggccagcagt tcctccggct tgccgctcat
     3241 cggcagcttc cggactgcaa gcgagaacac cggcgtcgca gcgtcggaga gtgccgatgc
     3301 gacggcttcg ccgataccgc cttcggcata gtggtcttcg acggtgatga tggccgaggt
     3361 ctcggccgtc gccctgtgca acgtcgtcag gtcgagcggc ttgacgctgt agaggtcgat
     3421 cacgcgggcg tggatgccct cggcagccag tgtgtccgcc gccttgaggg cttcatggag
     3481 cgtgattcct gcgctgacca gggtcacgtg gtcgtcgtca gcgtggcgga gggtcttgct
     3541 gccgccgatg gggaactcct ctccggtctc gtagagtcgc ggcatctcct gccgcgtggt
     3601 ccgcatgtag cagatgccgt gatgatccgc catctgcctg acgagagctg ccgttgaggt
     3661 gtcgtctgcc gggtacagca cgacgctgtc caccagcgtg cggaacatgg ccatgtcctc
     3721 cagacccatc tgggatgggc cgtcaggtcc gatggcgacg ccgacgtgcg accccacgaa
     3781 cttgatgttg gcccgatcca tggcgtactg tgccatgcgg atctggtcga aggcgcggga
     3841 gaagaaggcc gcgaacgtcg agacgaatgg tatcctgccc ctcgtgctga atcccagcgc
     3901 ggcagacacc atattctgtt cggcgatgta catctcgaag aaccgctcag gatacgtatt
     3961 tcggaaggta gcggcaaacg tcgagttgct gacctcggca tccagcacca ccatgtcggc
     4021 gtgggtcggg gcgatgtcca ccagggcccg cccgtacgcc tcgcggggcg acaccaccct
     4081 ggagtcggac gattgatcga cccgaacggg agcccacatg gccggcgttg cccggactgg
     4141 tttctcgatg gtgccaaggg tgttcagatc gatcggcccc agttcgagga ccgcacggtc
     4201 gaattcctcg cctttcagcg tcttgccgtg ccatccgtcc ttgttctcga ggaacgagac
     4261 gcccttgccc ttgatagtca tggcgatgag cacggtaggg cggccgcagg catggtcgat
     4321 ctcaccatac gcggcctgga ccgcgtccat gtcgtgcccg tcctcgagga cgatcgtgtg
     4381 ccagccaaag gcggcaaagc gtgcttcata ggtgtccagg ccccagccca gcattgtctc
     4441 gccgcgctgt cccagccggt tgacatcgag aatgcccacc aggttgtcca gcccgtagtg
     4501 cgacgccaac tctgcagctt cccagactga cccctcagcg acctcgctat cgcccatcag
     4561 gacgtaggta ttgtatggca gcttgtcgac gaatcgcccg ttcatggcga taccgacacc
     4621 gatgcccagg ccctggccta gtgagccggt cgcggcttcc gcataggcga agcgcggcgt
     4681 cgggtgtccc tcgaggggtg accccatctt gcgatatgtg agcaggtcct cctgcgacag
     4741 gacccccgcg gccgcataca gtgcgtagaa cagtggcgag gcgtgtccct tggagaagat
     4801 gacgcggtcg ttgcacgggt tctgtggttc gtccaggtcg aaatggaacg tgccgccgaa
     4861 gaacaggccc gtcatgaggt cagccgccga caatgaggtc gtagggtggc cggaccccgc
     4921 ttgcgtggac atgtgcagga tgtcgtagcg gacacgtgct gcaatcttcg tcagtattga
     4981 cgtctccatt ggtgcctccc aggccggaca agccggttgc tgtcaccagt gtgacccata
     5041 gccgactcca tacaagaacg tgttgtgtga gtctgtcggt ccaccccgga tgagggggcg
     5101 actgcatgaa tac
//
LOCUS       DATMZD010000086         2556 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_52946,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000086 DATMZD010000000
VERSION     DATMZD010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52946"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..891)
                     /locus_tag="VN478_01780"
     CDS             complement(<1..891)
                     /locus_tag="VN478_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21440.1"
                     /translation="MQAEGNGRPSTRIVSLIPLLAILAYLTFTVILFRTGPWPWPVAD
                     TTKLMLFLLACHGMFMLGYLGGSKLALPGVWSGEGFERRLLWGCIIVGAVLVLPTSYA
                     RTGHILPPLISALRNPGAAYSAALRWQSALGAAVIVEYVRVLVGPFLFAVFPLTTYYW
                     DTISPAEKISGLAVTTGYLLIGLSTGQNATVAEFVLLMPWIVIAGIWSKHIRVSRVRK
                     WAAIALILVMLLSLLVSFSRGQQTRSGSYNAAGWFMWTKTRADTNNWFVRVFPRRLQA
                     GALGLASYLSQGYYALSLALD"
     gene            complement(891..2168)
                     /locus_tag="VN478_01785"
     CDS             complement(891..2168)
                     /locus_tag="VN478_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21441.1"
                     /translation="MADSYDVKRLLMVPVRRWRAVVAVALIPTLIVGVLVMLVAPRRY
                     SCTATLLVAYEKPSAVVQDAGKISDYLASLPGSPLAAYQALLANPAFVRGIWGELGLD
                     KSPWKADSSRVRGIVRLVEVPNANLINVVVSFTDATKAAEIANAFAQGFAEWTNGLIK
                     TQPAEVVAEVPRQFHAAAKTLQASEKALGSIRAQQGGVEELTAMQSGLIADEAAYRSA
                     LTNLQVQRDMNGAILANKQVALAHEPTVLVTRKSIINDPALMAIAESQSGLSAEQLAS
                     LVMRSEEMNPVWESLRKDIVSLQVDIASAEKYRVIYEAAVQGIEKKLAETSAQLEDRK
                     AAVVEAERAVSLAQRQYDLAVATYESVINVSENAVPPIRVVLNATPADHADGDRTLKV
                     AFAFLASLLLGVVCAYVADAMTPPRSPGDKAGT"
BASE COUNT          562 a          828 c          692 g          474 t
ORIGIN      
        1 gtccagcgca agagaaaggg catagtaacc ttgggagagg tatgaggcaa gcccaagcgc
       61 tccggcctgc agacgccgcg ggaacacccg aacgaaccag ttgttcgtgt cagctctcgt
      121 cttggtccac atgaaccacc cggcagcatt gtagctgccg gaacgcgtct gctgacctct
      181 gctgaaggac accaggagac tcagcagcat aacgaggatc agcgcgatcg cggcccactt
      241 tcgcacacgg cttacacgga tgtgcttgct ccagatgccg gcaatgacga tccatggcat
      301 caacaggaca aactccgcaa ccgtcgcgtt ctgcccggtc gacaatccaa tcaggaggta
      361 gcccgtcgtg actgccaacc cactgatctt ctctgcaggc gatatggtgt cccaatagta
      421 ggttgtcaga ggaaacaccg caaacagaaa gggccccacg agcacccgca cgtattccac
      481 aatgacggcc gcacccaagg ctgactgcca tctaagtgcg gcactgtatg ctgcacctgg
      541 gttccgcagc gcagaaatca gaggtggcag gatgtgcccg gtgcgtgcat atgatgttgg
      601 cagaaccagc acagccccca cgataataca gccccacaga agccgacgct caaacccttc
      661 gccactccac acgcctggaa gggccaattt gctgccgccc aggtagccca gcatgaacat
      721 gccgtggcag gccagaagaa acagcatcag tttcgtcgtg tctgcaactg gccacggcca
      781 aggccctgtc ctgaagagga taacggtgaa tgtcagatag gccaggatag caagaagcgg
      841 aatcagagag acaatcctgg tcgaaggccg cccattcccc tccgcctgca ctatgtacct
      901 gctttgtcgc cagggctccg aggaggcgtc atagcgtcgg caacgtaggc acatacgact
      961 cccagaagga gcgatgccag aaaggcaaac gcaaccttaa gggtcctgtc gccatcggcg
     1021 tggtctgcgg gggttgcgtt gagcactaca cgaatcggag gaacagcgtt ttcgctcacg
     1081 tttatgacgg attcgtacgt cgcgaccgcg aggtcatatt gccgctgggc caggctgaca
     1141 gcacgctccg cctccacgac ggccgctttc cggtcctcga gctgcgcgga ggtttctgcc
     1201 agcttcttct cgatgccctg caccgctgct tcgtagatga ccctgtactt ctccgcactg
     1261 gcaatatcca cctgaagact gacgatgtcc ttcctcaggc tctcccagac gggattcatc
     1321 tcctcagacc gcatgaccag ggaggcaagc tgctcggctg acaaccccga ctgagactcc
     1381 gctatcgcca tgagcgcagg atcattgatg atgctcttcc tggtgaccag cacggtaggc
     1441 tcgtgcgcca atgcaacctg cttgttcgca agaatggctc cgttcatatc ccgttgcacc
     1501 tgaaggttgg tcaacgcaga gcgataggct gcctcatcgg caatgagtcc actctgcatg
     1561 gcggtcagct cttccactcc tccctgctgg gcacgaatac tgcccagcgc cttctcgctg
     1621 gcctgcagcg tctttgcggc ggcatgaaac tggcgaggca cctcggccac gacctcagca
     1681 ggttgagtct tgatgagccc gttcgtccac tcggcaaacc cctgagcaaa tgcgttggcg
     1741 atctcagccg ccttggtggc atctgtgaaa ctgaccacga cattgatcag attcgcgttc
     1801 ggaacctcga cgagtcgcac aatgccgcgg actcgactgc tgtccgcctt ccacggcgat
     1861 ttgtcgagac caagctcccc ccagatcccc cgcacgaacg cggggtttgc caacagcgct
     1921 tgatacgccg caaggggcga accgggcagg ctggccaggt agtccgagat cttcccagca
     1981 tcctgcacta cagcgctagg tttctcatag gccacgagca gggtagccgt gcaggagtat
     2041 cttctggggg cgaccagcat cacgagcact cccacgatga gagtcgggat cagcgcgacc
     2101 gcgacaacgg cccgccagcg ccgtaccggc accatcaaca gacgtttcac atcatatgag
     2161 tccgccatca cgcccctctg ttctcgtaca cgtcacacaa cgccccctcg agctcagccg
     2221 aggtccacat gcaagcacca atcaggcaca aagctaattc ccatactacc tgttcaggat
     2281 gctctgtcaa tgcggtcagc ccagccccac tccccagcaa tgccgctggc acgcagcaac
     2341 agatgccgcg tgccagcgtg acgaccctct ttagagttga ctactcgaga tgcactagaa
     2401 tatcactacc tgcctggcta tcctcgggat catggtggaa gcaccgtaga cgacggggtc
     2461 agcgacgccg ttcagccaga agaagagtgc tccgctgcgc tgctcgactg caccgacttc
     2521 atccgtgcca tcaccgttcc agtcaccgac gatcgg
//
LOCUS       DATMZD010000087         2756 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_62781,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000087 DATMZD010000000
VERSION     DATMZD010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62781"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VN478_01790"
     CDS             <1..237
                     /locus_tag="VN478_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HWQ21442.1"
                     /translation="IGAMQAIEFVKNRTTREPATQLNGEIIKECLHNGVLVAGSGIYG
                     NCIRMLVSLEITDEQLKEAFAVMETAVARANASR"
     gene            complement(312..773)
                     /gene="dtd"
                     /locus_tag="VN478_01795"
     CDS             complement(312..773)
                     /gene="dtd"
                     /locus_tag="VN478_01795"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002648659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HWQ21443.1"
                     /translation="MRAVVQRVSSASVTVDGQEVGRIGAGLLVLLGVAPDDGPEDTAF
                     MVRKLGGLRIFGDADGLMNCSIADVHGSFLVVSQFTLYGDVARGNRPSFTAAAAGAYA
                     DEIYKNVCAGLRNQGFDVQNGVFGAHMDVSLVNDGPVTIVMETDSRKRRSE"
     gene            903..978
                     /locus_tag="VN478_01800"
     tRNA            903..978
                     /locus_tag="VN478_01800"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:936..938,aa:Ala,seq:ggc)
     gene            complement(1099..1302)
                     /locus_tag="VN478_01805"
     CDS             complement(1099..1302)
                     /locus_tag="VN478_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21444.1"
                     /translation="MPDMPVEKVKQRIAELRALKRTTLQERAVVDMERAQLDEIRGVL
                     ADCEPFFARLDRDRASWKSCAGL"
     gene            2300..>2756
                     /locus_tag="VN478_01810"
     CDS             2300..>2756
                     /locus_tag="VN478_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWQ21445.1"
                     /translation="MQIANFTHSHIVQAQALAKQNYKEERGFVTALPQIDSMPDLIEF
                     ADNNMGVAAFENCEMVGFMCAYSVFDDAFGTTGVKGTWSPLHAHAAVGDKEKIYKRLY
                     QAAAEKWVAAGAVSHAITLYAHEKDALDSFFTYGFGLRCVDAIRAIDDVE"
BASE COUNT          620 a          814 c          703 g          619 t
ORIGIN      
        1 ataggcgcca tgcaggctat cgagttcgtc aagaatcgca ccacccgcga acctgcgacg
       61 cagctgaacg gcgagatcat caaggaatgc ctgcacaacg gcgtcctcgt agccggttcg
      121 ggcatctatg gcaactgcat ccgcatgctt gtttcactgg agatcaccga cgagcagctg
      181 aaagaagcct ttgctgtcat ggagacagcc gtcgctcgcg ccaacgcgtc gcgctgatcc
      241 gctcgatctc caccaatact acaagaagcc ggggcaaatg ccccggcttc ttgtttgcag
      301 aactatctgt cctactctga tcgcctcttg cggctgtccg tttccatgac gatcgtgact
      361 ggtccgtcgt tcaccaggct gacatccatg tgtgccccga acactccgtt ttgcacgtcg
      421 aaaccctggt ttcgcagacc ggcacataca ttcttgtaga tctcatccgc atacgctcct
      481 gcggcggcgg ccgtgaagct gggccggttg ccgcgggcca cgtctccata cagcgtgaac
      541 tggctgacaa cgagaaacga tccgtggacg tctgcgatcg agcagttcat cagaccgtct
      601 gcgtctccga agatgcgcag accacccagc ttacgcacca tgaatgccgt gtcttcgggt
      661 ccgtcatcag gcgccacgcc caacaacacg agcaaccctg ctccaatacg ccccacttcc
      721 tggccatcta ccgtgactga agcactgctc actcgttgca ccactgcccg cacacgtcac
      781 ctctcacata gcttgagtca ggcactcata ctagcacgac cagcctgcaa cgcgaatcat
      841 gccgcttccg gttgacactg gggtccttcc cgctatactt ttggtgttcg tttccaagac
      901 gtggggatat agctcagctg ggagagcgcc tgcatggcat gcaggaggtc gggggttcga
      961 gcccccctat ctccaccact cgacccctta cacctacttt tctcgctctg ggactgactt
     1021 ccagccgagg caccttgcac tcattttccg agaatctcca gtggataact atgttagtaa
     1081 ctcccaggtc gatgcgtctc acaaacctgc gcacgacttc cagctggctc tgtcgcggtc
     1141 cagcctcgcg aagaacggct cacaatcagc caagacaccc cggatttcat ccagctgcgc
     1201 ccgctccatg tcgactaccg cacgctcctg gagtgttgtg cgtttcagtg cacgcagctc
     1261 tgcgatgcgc tgcttcactt tctcaacggg catgtctggc atgacaagtc gaccttcgac
     1321 cacgacaatg cccctatcgc acgtctcagc tcttgcatca agtgcagcta gacgctctgt
     1381 cccatcgaat cgggggtgag aacccccgca gttcgacact tactttcccg aggaccacgg
     1441 ggcgcagatg gtgctcccag agttgggccg cgcacgcttt ctgcgctggt cccgaactct
     1501 gacaagcgac ttggtcttct tccctttggc gttgccatgt gcgctgagga gggaccgcac
     1561 tccgtgcact acaagagcct cccgaacagg ggcaacgtgt gcccctcgct tctgtccgag
     1621 cgagcagcct tgaccagtcc tgccttgctg gcactcatag acatcgagca acgagaaggc
     1681 cttgacacat ccatgtaccg aacggacttg tgggccctac actaaggttg gtacgttctt
     1741 ctcgcaggag gtgtactggt gttcttggcc agcctactcc gaggccgcag caagaaacag
     1801 gacacctcgc ccgttccacc agaaaccctc tgacgcaatg accggggaca ggatgacatt
     1861 gctatccact tcgaagctgc gttcgccacg ttcgtctgaa ctgacctcaa gatgtgcatc
     1921 agataaacga aatcgatgca gaagtcgcac catctcttga ttcggagcac ttctgtgaca
     1981 gactgctttt ggaagatagg actcttgatg caggtgcgaa atgtcttgga tagaccgtcg
     2041 cttgggactg acagatgaag ggaacgaacg agtagaatgc ccgaaagaag aacgtccatg
     2101 ggaagaggcc caacctgtct caccacttcc tccagttgta atgacattgc tcttgatact
     2161 gatcttgaaa acaaacgggc gcagcatgta tatgacaaac tgggtttcgt tagaacaagg
     2221 acggatatcg attcttggaa aagccaactt ggagaactgc agtcgcaagt atgctatgaa
     2281 ttgacgaagg agaaccttca tgcaaatagc caattttaca cactcccaca tagtacaggc
     2341 acaagcactt gccaaacaga attacaaaga ggagcggggc tttgtcactg ctctgcctca
     2401 gattgattcc atgcctgacc tcattgaatt tgccgacaac aatatggggg tcgcagcctt
     2461 tgaaaactgc gagatggtcg ggtttatgtg tgcctattct gtgtttgatg acgcattcgg
     2521 taccactggt gtaaaaggta catggtcgcc gctccatgcg catgccgctg ttggagacaa
     2581 ggaaaagatc tataagcggc tatatcaagc ggcagccgaa aaatgggttg ccgcaggagc
     2641 ggttagccat gcgattacgc tctatgctca tgaaaaggat gcacttgaca gttttttcac
     2701 ctatggattc ggtctgcggt gtgttgatgc catccgtgcc attgatgatg ttgaga
//
LOCUS       DATMZD010000088         2384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_103456,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000088 DATMZD010000000
VERSION     DATMZD010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103456"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="VN478_01815"
     CDS             complement(<1..460)
                     /locus_tag="VN478_01815"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWQ21446.1"
                     /translation="MNSKTQTVQERIARLIDVAAGHTPADVVVRNVHLVNVFDGSIEE
                     DVDIALSGGQVAGIGRYDGVTVVDGRGAYAAPSFIDAHMHIESTMVVPGEFAKVALAC
                     GTGAVIADPHEIANVAGARGIRFMIEATRNSPLDFFFMLPSCVPATHLETN"
     gene            complement(471..1805)
                     /locus_tag="VN478_01820"
     CDS             complement(471..1805)
                     /locus_tag="VN478_01820"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWQ21447.1"
                     /translation="MNKLLIKNGTLLTMGGDRQPRKVDLLVTDGRIKRIDTAMDRGEH
                     PDVTIDATGMIVLPGFIFGHARLGFSLLRGQTDQILDDAERERTALRLVAGLSPEQVR
                     CSAELGICQAVRSGVTTLLEGGAMRHTDQIFQAARSLGFSLIAGRMLADRTPDWPEAL
                     HMSLREQMSDAQSLAHDIASNDGNGLLHYCVSTTNPLFCTDECLTQAKTLADSGDYPL
                     KIVLTTDKSQIERMRRESGPQELASLEALGVLGEHTFLVNPSYTSELEQNVIRKAGAR
                     VVINVTADLKLGRPPSRLPEFVRKGTPVLPGFESPLYGGRLDAIREIAVLATAFRSQY
                     GPQTMTPEQVLSMVTVDAAKALGMDEDIGTLEQGKRADIVLVDLGRDIFAQPGAHTNA
                     YCRLVYEASAGNVAYTIINGNIVYRDGHVTGCDEEALLEQANTELVHILEKA"
     gene            1903..>2384
                     /locus_tag="VN478_01825"
     CDS             1903..>2384
                     /locus_tag="VN478_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21448.1"
                     /translation="MSQTVMIEALGQGSEARRHALDSVFGWMQPQGLAGKRVLVLLGD
                     APVQMDLVERTFQTLSSAARVDLAAKSLAGYDEEYRSALKGLLGERGDLIETLAGEYE
                     ALAVPTRSYARELRKMETPERRYIKQAFVARCLAEADLFVVVRRLELNRFSGVHGVFA
                     T"
BASE COUNT          474 a          671 c          733 g          506 t
ORIGIN      
        1 cgttcgtctc aaggtgtgtg gcagggacac aggatggaag catgaagaag aagtccagcg
       61 gcgagttgcg tgttgcctcg atcatgaacc ggatgccgcg ggctccagcg acgttggcga
      121 tctcgtgcgg gtccgcgatc acggcgcccg taccacacgc gagtgccacc ttggcgaatt
      181 cgcccggaac gaccatggta ctctcgatat gcatgtgagc gtcaatgaag ctgggagcgg
      241 cgtaggcacc cctgccatcc acgactgtca caccgtcata tcgtccaatt cctgctacct
      301 gaccgcctga cagcgctatg tcaacatctt cttcgatgct gccgtcgaag acgtttacca
      361 gatggacgtt cctgacgacc acgtccgccg gagtatggcc ggcggcaacg tcgatcagtc
      421 gggcaatgcg ttcctggact gtctgagtct tgctgttcat gagccttccc ttacgccttc
      481 tccaggatgt ggacaagctc cgtgtttgcc tgttcgagaa gcgcttcttc gtcacagccg
      541 gtgacgtggc catcacggta gacaatattc ccgttgatga tcgtatacgc cacattgcct
      601 gcagatgcct catagaccaa tcgacagtag gcattcgtgt gagcaccggg ttgagcgaat
      661 atgtcccttc caaggtccac gagaacgatg tcggctcgct ttccctgctc cagcgtcccg
      721 atatcctcat ccatgccgag tgcctttgca gcgtccaccg tcaccatcga gagaacctgc
      781 tcgggcgtca tagtctgtgg accatattgt gaccgaaacg cagtcgccag cacggcgatc
      841 tcgcggatcg cgtccagtct gccgccgtac agagggctct cgaaacccgg gagaacgggc
      901 gtccccttgc ggacgaattc cgggagtcgc gatggcggac gaccaagttt caggtcggcc
      961 gtcacgttaa tgacgacacg ggcgccagcc ttgcgaatga cgttctgctc caattcggag
     1021 gtatatgacg ggttgaccag gaacgtatgt tcgcccagaa ccccgagcgc ttcaagagaa
     1081 gccagttcct gaggtccact ctcgcgacgc atgcgctcga tctggctctt gtcggtggtc
     1141 agtacgatct tgagagggta gtcgccggag tcagcgagcg tttttgcctg agtcaggcac
     1201 tcgtctgtgc agaacagcgg gtttgtggtg ctaacacagt agtggagcaa gccgttgccg
     1261 tcgttcgatg cgatgtcatg tgcaagtgac tgcgcgtcag acatctgttc gcggagcgac
     1321 atgtgcaggg cttcaggcca gtctggcgtg cggtcagcca gcatgcgtcc ggcgatgagg
     1381 ctgaatccga gcgagcgcgc cgcctgaaag atctggtcgg tatggcgcat ggcaccgcct
     1441 tcgaggagag tcgtgacgcc actgcgaaca gcctggcaga ttccaagttc agccgagcag
     1501 cggacctgtt cgggcgaaag acccgcgaca agcctgagcg ccgttctttc gcgctcggca
     1561 tcatccagga tctggtcggt ctgaccgcgg agaagactga agcccaggcg cgcatgaccg
     1621 aaaatgaaac caggcaggac gatcatgcct gtggcatcga ttgtgacgtc agggtgctca
     1681 ccgcggtcca tagcagtatc gatgcgcttg atacgtccgt cggtgacgag aaggtcaacc
     1741 ttgcggggct gacggtcgcc acccatggtc agcagtgttc cgttcttgat gagcagctta
     1801 ttcataatgg ttgtgctgcc tattgtaccc tgcgtgcaac tctttgtcaa agcgcgaatg
     1861 ctgttgcgaa accaggtata ctgttgtcga caaggaggaa ccatgagtca gacagtcatg
     1921 atagaagcac tgggacaagg ttcggaagcc cgaaggcatg cgctggacag tgtgtttggc
     1981 tggatgcagc ctcaagggct tgctggcaag cgcgtgctcg tgctgcttgg cgacgcgccg
     2041 gtccagatgg acttggttga gcgcaccttc cagacactgt ccagtgctgc acgggtcgat
     2101 ttggccgcaa agtctttggc cggctatgat gaagagtatc gaagtgccct gaagggcttg
     2161 ctgggcgaac gtggggacct tatcgagacg ctggcagggg agtacgaggc attggcggtc
     2221 cccacaaggt cctacgctcg ggaactacgc aaaatggaga ctccagagcg tcgctacatc
     2281 aagcaggcgt tcgtggcgcg atgcctcgct gaggcggatc tgttcgtcgt tgtacgccgg
     2341 ctcgaactca acaggttctc gggcgtccac ggtgtctttg cgac
//
LOCUS       DATMZD010000089         7870 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_42714,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000089 DATMZD010000000
VERSION     DATMZD010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7870
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42714"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1230)
                     /locus_tag="VN478_01830"
     CDS             complement(<1..1230)
                     /locus_tag="VN478_01830"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HWQ21449.1"
                     /translation="MSRFSPSDIRNFALVGQGGSGKTMITERLVFHAGASQRVGRVED
                     GTTVSDYLDEEIKRHISTGIGLCEFDYKGVRLNLLDTPGYADFMTEAQEAVRVADTAL
                     LVINAGAGIEVQTPRFWGLCEAQHIPVAFVVNKLDLENVDFGTRVAELQDAFGHKVAA
                     LTIPIVSGPALSSFVNVLTGKVHTYASGKDEAILSDAIPDSVRSDYEAARVALTEAVA
                     ESDDATMEKYLGGEALTQDELVMVFTKAFKKREVFPVLGVSALKGISCDYLMDFLSSY
                     APSPVEIAVSATRTGTDEIVELKADPAAHAAAFVFKTTSDPFVGKLSFIRVYSGRIAH
                     DVYNGTKDVSEKVGGLFHMIGKKQEPADEGIAGDILVISKLVQTATNDTISTKDVPLD
                     LVPITFSPPLLMRSLKPK"
     gene            complement(1252..5433)
                     /locus_tag="VN478_01835"
     CDS             complement(1252..5433)
                     /locus_tag="VN478_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013433114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21450.1"
                     /translation="MAGDIIDFTKINSSYAMPDLLQLQKTSYDWLLGPGLAQIFARVF
                     PIDTAKLRIEYVSHDTERPAKSMENCRKAGLSFEAMVRANFRLINKETGEIREQPLII
                     TQIPLMTPYGTFLINGAERVIVSQLVRSPGVYFEGGKDKLSGAQIYMVTLIPDRGNWM
                     EIEAGKDDVIYTRLRKKARKVPMTLVLKALGVESNEEIERLFTIEKTERLSTEQLRSV
                     FNQAEYTAVEPLLDSKGKEIIKEGALITGKAMSLLENHGVSSFLVSSKQPDRWVVKTL
                     KADKTANQEEAIKQIFKEMQPKERLTLEAAQSLLSTLLTDQAYNTFTPIGRYKINKRF
                     GAETNSAVLTKDDYVSIIRHLIDIRERKANFDDIDSLANRRIRRVGELLAEEIQDSML
                     KVAKATRDKVTIYSSEQMMNLQNVLNTKPVISSIRNFFGQSQLSQFMEQINPLSSLAH
                     KRRLSSLGPGGLNRKRAGVEVRDIHSSHYSRICPIETPEGGNIGLINSLSSYAKLDEY
                     GFITAPYAKVENGHKDEKNVVYMDAEEEEYHSIAQANTAVDEKSRLTSKTVIARQRSE
                     GGEILPKELPAGKVDYIDLLPSQIFGISASLIPFLEHDDANRALMGCNMQRQAVPLVR
                     PDRARVSTAMEGRVVHDNGDLVLSDADGIVSYVDAEQIRVSPLVSVADLQSGSIPVDV
                     VPVMLASDANEPGILGDVRMVPISFSPAGVPESILSLIGTEISHEQIETLSDDGVESF
                     QYVPAGSIVLNSLTDLFDVQTNDGLLGKVLAKDVQEGKNKVHKAGTKITETLLGRLIN
                     NAKIRSLPIQEGDETAQVELQMLTMTPIGQQVLGHRILGVTSALDLGSHVGRTVGEES
                     LRDLFAKDRNASILTYDPAKAAAYELFRVNGSLAGQTLAEDVQLRKPKARKLSIGRKL
                     TRDDLILMAKNGVTHVSVPQARVFELQKFKRTNQDTCINQHPAVSTGDEVHVGDLLAN
                     GYASAEGELALGKNLLVAYVPWRGYNYQDGVIVSERLVRDDSLSSIHIKEHKIEVRST
                     EFGKEEITRDPPNVSKELARHLDERGVVQIGAHVKAEDVLVGKITPRAEQEESPEVRV
                     YFALFHDKARDYKNTSYKVPPGEDGVVIDVQEITKDNTSDALPVGVERIIKVYVAKKR
                     KIIVGDKVAGRHGNKGVITRILPEEDMPFLQDGTPVDVILSPLGVPSRMNIGQLLEAS
                     LGIAARKLSVRAVTPVFNGATEEEIKALLRKAGLNDSGKEDVRDGVYGKYFDSQVTVG
                     EAYILKLIHLAEDKMHARSTGRYTLITQQPIGGKAQFGGQRFGEMEVWALEAYGASTV
                     LQEMLTIKSDDLEGRRVAYERLTKGNNLFETGSPESFNVLVKELRGLGLDLETLPKAG
                     DTHAKSTKVVLEDNE"
     gene            complement(5692..6360)
                     /locus_tag="VN478_01840"
     CDS             complement(5692..6360)
                     /locus_tag="VN478_01840"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWQ21451.1"
                     /translation="MKIAAVIPAFNEEHNIAGVVEGVRLFMADIVVVDDGSTDGTAAA
                     ARGAGAIVLRHESNVGKGAALKTGFGYLRESGFDGAITIDADGQHDPAEIPLFVAAAE
                     QGYDMVVGNRMSNVSTMPFIRRFANHVSSFLISLFLGQNVRDSQNGFRYYRLSSILSL
                     PLKADKYDLETEVIFKVGRARYRIGWVPTRTIYRAEARSKFNSVTDTLRFIGALVRYG
                     LLRE"
     gene            complement(6357..7214)
                     /locus_tag="VN478_01845"
     CDS             complement(6357..7214)
                     /locus_tag="VN478_01845"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWQ21452.1"
                     /translation="MAFWYVVAFRVAAILPPWLKRTIAKTIALGYFVFNARQRRGVMA
                     NLTIMRPEAGRSVLLRLTVRTFRQASLNLVDFFGIPSMSDERVRSLAVNFDDMRTLLE
                     QKSGGEAFCLVAAHYGHWELAGAVIGLAGYKTHAIALTQNSPVVERLYESLRVRFGLT
                     AHDIRYGLRHLLRSLPEGEVPAIVSDREYGAGGEEVLFFGRRVSFPRGAAVLCYRRHL
                     VGIPGFLVRRPDGRYVLKFGDPIRPEPADERSWVRSFVGDFARQYERIVDEDPTQFLN
                     FYDFWGTAA"
     gene            complement(7218..7778)
                     /locus_tag="VN478_01850"
     CDS             complement(7218..7778)
                     /locus_tag="VN478_01850"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HWQ21453.1"
                     /translation="MRTRERMFETVVSCRRAYLGKSIELLDKVVRLPDDRFAHRDVVT
                     YAGGAVCVVAVAADNRIVLVRQYRPAPETVMLEIPAGRRGVRERFLTAARRELREETG
                     YTAGSWKKCVEFWPSPGFVDERLTLYVARDLQPGPTAMDSDEFIRPILIPVGEAMERC
                     RNGLINDAKTLIGLLWYGAFEGSAGR"
     gene            complement(7775..7864)
                     /locus_tag="VN478_01855"
     CDS             complement(7775..7864)
                     /locus_tag="VN478_01855"
                     /inference="COORDINATES: protein motif:HMM:NF013935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21454.1"
                     /translation="MRSITSKLDENERANTTRLLKIKEMIRSH"
BASE COUNT         1642 a         2345 c         2095 g         1788 t
ORIGIN      
        1 cttgggcttg agagaacgca tgaggagagg tggtgagaac gtgatgggca ccaggtcgag
       61 aggcacatcc ttcgtcgaga tcgtgtcgtt cgtcgcggtc tgtaccagtt tcgagatcac
      121 cagaatgtcc ccggcgattc cttcgtcggc cggctcctgc ttcttcccaa tcatgtgaaa
      181 gaggccaccg accttctccg agacatcctt cgtcccgttg tagacatcgt gtgctattct
      241 tccactgtag acccggatga acgacagctt gccgacgaac gggtctgagg tcgtcttgaa
      301 tacaaaagca gctgcatgcg cagctggatc tgccttgagc tcaactattt catcagtacc
      361 cgtgcgggtc gccgacactg caatctcgac gggcgacgga gcatatgatg acaaaaagtc
      421 catcaggtag tcacagctaa ttcccttcag ggctgagaca ccaagaaccg ggaagacctc
      481 tcgcttcttg aacgcctttg tgaagaccat cacgagttcg tcctgggtca gagcctcgcc
      541 accaagatac ttctccatag tggcatcatc cgattcggca actgcctccg taagggcgac
      601 gcgtgccgcc tcgtagtccg atctgacgct gtcggggatg gcgtcggaca gaatcgcctc
      661 gtccttgcca gaagcatagg tgtggacttt gcccgtgagg acgttcacga acgaagatag
      721 agcagggccc gagacaatcg ggatggtcag agcggcaacc ttgtgtccga aagcgtcttg
      781 cagttcggcg actcgggtac cgaagtcaac attctccaga tcaagcttgt tgacgacaaa
      841 tgcgacggga atgtgctgcg cttcgcacag gccccagaaa cgcggcgtct ggacttcgat
      901 gccagctccc gcattaatca cgagaagtgc agtgtcggct accctcaccg cctcctgtgc
      961 ttcagtcatg aaatccgcat accctggggt gtcgagcaga ttcagccgaa cccccttgta
     1021 gtcgaactcg cacagaccaa tgcctgtgct gatgtgccgt ttgatctcct cgtccaggta
     1081 gtcggaaaca gtagtcccgt cttcgacgcg gccaactctc tggctcgcgc cggcatggaa
     1141 caccaggcgt tctgtgatca tcgtcttgcc agagccgcct tgtccaacta gcgcgaagtt
     1201 ccttatgtca ctcgggctga atctgctcac cttttcctcc cctagagaat gttattcgtt
     1261 gtcttccagc acgaccttgg tactcttcgc gtgagtgtcc cctgccttcg gcagtgtctc
     1321 caggtccagt ccaagcccgc gaagctcttt cacaagaaca ttgaatgact cgggagagcc
     1381 cgtttcgaac aggttgtttc cctttgtgag acgttcatat gccacacgtc tgccctcaag
     1441 atcgtctgac ttgatcgtca gcatctcctg caggacagtc gacgcgccgt acgcctccag
     1501 ggcccacact tccatttctc caaaacgctg cccaccgaac tgagctttgc ctccgatcgg
     1561 ctgctgcgtg atcagcgtgt accgaccggt agaacgcgca tgcatcttgt cttcggcaag
     1621 atgaatgagt ttaaggatgt acgcttctcc tacggtaacc tggctgtcaa agtacttgcc
     1681 atatacacca tctcgaacgt cctctttgcc actgtcattg agccccgcct tccggaggag
     1741 tgccttgatc tcctcctcgg tcgctccgtt gaacacaggc gtcacggcac gcacgctcaa
     1801 cttccgcgca gcaataccca gactggcttc gagtagctgg ccgatgttca tgcgcgaagg
     1861 cacgccaagc ggactgagga tgacatcaac aggtgtgccg tcctgcagga atggcatgtc
     1921 ttcttccggc agaatgcgcg ttatgacacc cttgttcccg tggcgtccag caaccttgtc
     1981 tccgacaatg atctttcgtt tcttggcgac atagaccttg atgatccgct cgacgccgac
     2041 aggcagagca tcactcgtgt tgtctttcgt gatctcctgg acgtctatga caaccccgtc
     2101 ctctccgggt ggcaccttat aggacgtatt cttgtagtca cgagccttgt cgtggaacag
     2161 ggcgaagtag acgcgcactt cgggcgattc ctcctgttcg gcacgcggtg tgatcttgcc
     2221 taccaggaca tcttcggctt tgacgtgtgc tcctatctgt accacaccgc gttcatcgag
     2281 atgccgcgcg agttccttgg acacattcgg aggatcgcgg gtgatttcct ccttgccgaa
     2341 ctccgtcgac ctgacctcga tcttgtgctc cttgatgtgg atggacgaga gactgtcgtc
     2401 acgtacgaga cgctcgctga caatgacacc gtcctgatag ttgtatcctc gccacggaac
     2461 ataggctacc agaaggttct tgcccaaggc aagctcgccc tctgcggatg catagccgtt
     2521 tgccagaaga tcaccaacat gcacttcgtc tccagtcgat acagccgggt gctggttgat
     2581 gcacgtatcc tggtttgtcc gcttgaactt ctgaagctcg aaaacgcgag cctgcggcac
     2641 cgagacgtga gtcacgccgt tcttcgccat tagaatcagg tcgtcgcgag tcaacttcct
     2701 cccgatggac aacttcctcg ccttgggttt gcgcaactgg acgtcctcag ccagcgtctg
     2761 tcctgcgagg gatccgttga cccggaagag ttcataagct gctgccttcg cgggatcata
     2821 ggtaaggatg ctagcgttcc tgtcctttgc gaagaggtca cgcagactct cttcaccgac
     2881 agtgcgtcca acgtgtgaac ccaggtccag cgcagatgtt actcccagta tgcgatgacc
     2941 aagaacctgc tgaccgatgg gagtcatggt gagcatctgg agttcgacct gtgcagtttc
     3001 gtcaccttcc tggatgggga gggaacggat cttcgcattg ttgatgaggc gaccgagcag
     3061 agtctcagtg atctttgtgc ccgccttatg caccttgttc ttgccttcct gaacatcctt
     3121 ggcgagcacc ttccccagca gaccgtcatt ggtctgtacg tcgaacaggt ccgtcaggga
     3181 gttcaggacg atagagcccg caggaacgta ctggaacgac tcgactccgt cgtccgacag
     3241 agtctcgatc tgttcatgcg agatctccgt cccaatcagg ctgagaatgc tctccggaac
     3301 accagctggg gagaacgaaa taggcaccat gcggacatcg ccgagaatgc ctggttcgtt
     3361 ggcgtcggag gcgagcatga cggggacaac atctacggga atgctaccac tctgaaggtc
     3421 tgctacactg accaaaggag agactcgaat ctgttcggca tcgacatacg agacgattcc
     3481 gtccgcatcg ctgagtacga ggtctccatt atcgtgtact acccgcccct ccatcgcagt
     3541 cgacacgcga gcccggtcag ggcggacgag cggcactgct tgccgctgca tgttgcagcc
     3601 catgagggcg cggttcgcat cgtcatgctc aaggaagggg atcaatgaag cagaaatgcc
     3661 gaatatctgt gacgggagga gatcgatata gtcaaccttc cccgcgggaa gctctttcgg
     3721 gaggatttcg ccaccttcac tgcgttgacg ggcaatgacc gtctttgagg tcagacggct
     3781 cttctcgtcg acagcggtgt tggcctgagc gatcgagtgg tactcctcct cttcggcatc
     3841 catgtagaca acgttcttct catccttgtg accattctcg actttggcat agggagcggt
     3901 aatgaagcca tactcgtcga gcttcgcata tgagctcaga ctgttgatga gaccaatgtt
     3961 tccgccctcc ggagtctcga tagggcaaat acggctgtag tgggaagagt ggatgtcgcg
     4021 gacttccacg cctgcacgct tcctgttcaa gccgcctggt ccgagagaac tcaggcgcct
     4081 cttgtgggcc agactcgaca gggggttgat ctgttccatg aactgggaga gttggctctg
     4141 cccgaagaag ttcctgatgg aagagatgac aggcttggtg ttgaggacat tctggaggtt
     4201 catcatctgc tctgacgagt agatcgtaac cttgtcacga gtcgctttgg cgaccttcag
     4261 catactgtcc tggatctcct cggccagcag ttctccgact cgcctgatac gacggttcgc
     4321 caagctgtcg atatcgtcga agttggcttt gcgctcgcgg atgtcgatca ggtgtcggat
     4381 gatcgataca tagtcgtcct tcgtcaggac tgccgaattg gtttctgctc caaatcgctt
     4441 gttgatcttg taccggccta tgggtgtgaa cgtgttgtac gcctggtcag tcaagagcgt
     4501 cgaaagcaga ctctgcgcgg cttcaagagt gagacgttcc ttgggctgca tctccttgaa
     4561 gatctgtttt atggcctctt cctgattcgc ggtcttgtca gccttgagtg tcttcacaac
     4621 ccagcggtca ggctgcttgc tgctgacaag gaacgaactc accccgtgat tctcaagtag
     4681 gctcatcgcc tttcctgtga tcagggcgcc ctccttgatg atctccttgc ccttggaatc
     4741 cagaagcggc tccactgctg tgtactccgc ctgattgaac accgagcgaa gctgctcggt
     4801 ggacaggcgc tcagtcttct cgatagtgaa gagacgctcg atctcctcgt tcgactcgac
     4861 gccaagtgcc ttgagcacaa gagtcatggg gaccttgcgg gccttctttc gaagacgcgt
     4921 gtagatgaca tcatccttgc cggcctcgat ttccatccag tttccacggt caggtatgag
     4981 ggtcaccatg tagatctggg cgccggaaag cttgtccttt ccaccctcga agtagacgcc
     5041 gggcgagcgg acaagctgcg agacgattac acgttctgca ccgttgatga ggaacgtgcc
     5101 atacggcgtc atgagcggaa tctgagtgat aatgagtggt tgttcacgta tctcgccggt
     5161 ctccttgttg atgagacgga agttggcacg aaccattgcc tcaaaactca gccctgcctt
     5221 gcgacagttt tccattgact tcgcaggtcg ttcagtatcg tgactaacgt actcgatccg
     5281 cagtttcgcg gtgtcgatgg ggaagactct cgcaaagatc tgagcgagac ctggtcccag
     5341 aagccagtcg tatgaagtct tctggagctg gaggaggtcg ggcatcgcgt aacttgaatt
     5401 gattttggta aagtctataa tatctcctgc cacgcgcatg gctctccttt catatcgggt
     5461 tggaaaaaag cggtcaaatc aaaagtctac tctcttttgt ttgacagtca actgcgctct
     5521 ctccactatt ctggaggctt catcagcctg cctctcttgc caaggagacg tgtcttcaca
     5581 ttgtcatctc gcttgacccg ttgcgcagcc gaggatcagc ggttgcgact catgtgcgcc
     5641 atcacgttgc gtcgtcgaca cccccggcca ttggcgcaac aacaaccgga cctactccct
     5701 gagcagacca taccgcacca acgccccaat gaacctgagg gtgtcggtga cagagttgaa
     5761 cttgctcctt gcctccgctc ggtaaatcgt ccgagttggc acccagccga tacgataccg
     5821 tgctctgccc accttgaaga tgacctcggt ctccagatca tacttgtccg ccttcagcgg
     5881 cagagagaga atggaactca gcctgtaata gcggaaacca ttctgactgt cccgaacatt
     5941 ctgcccaagg aacaggctga taaggaagga tgacacatga ttcgcaaacc tgcggatgaa
     6001 tggcatggtt gagacattcg acatccggtt cccaacgacc atgtcgtagc cctgttctgc
     6061 tgctgcaaca aacaagggaa tctcggcggg atcatgttgt ccatcggcat cgatggtgat
     6121 cgcgccgtca aaaccgctct ccctgagata tccaaatcct gtcttcagcg ccgccccttt
     6181 gcccacgttg gactcgtgac gcaggacgat cgcacctgca ccccttgctg ctgcggctgt
     6241 accatcggtt gaaccgtcgt ctacaacgac aatgtcagcc atgaacagcc gtacgccctc
     6301 cacgacgcca gcaatgttgt gctcttcgtt gaacgcagga atgacagcag cgatcttcat
     6361 gcagccgtgc cccagaagtc atagaagttc aggaattgcg tcggatcctc gtcgactatc
     6421 cgctcatatt gacgagcgaa gtcccccacg aaggatctca cccagcttct ctcatctgcc
     6481 ggttccgggc gtatcgggtc accgaacttc aacacatacc tcccgtccgg ccgccgaaca
     6541 agaaagccgg gaatgcccac caggtgacga cggtaacaca gcactgcagc tccgcgcggg
     6601 aacgataccc tgcgcccgaa gaacaacact tcctctcccc cggctccata ctccctgtca
     6661 gacactatcg caggtacttc gccctccgga agactccgca gcagatgcct caggccatag
     6721 cgaatgtcat gggccgtaag gccaaaacga acccggagcg actcatagag gcgttcgaca
     6781 actggactgt tctgcgtcag cgctatggca tgcgtcttgt agcccgcgag cccaatcacc
     6841 gctccggcaa gctcccaatg gccgtagtgc gccgccacga ggcaaaatgc ttctccacca
     6901 gatttctgct cgaggagagt acgcatgtcg tcgaaattca ccgccagcga ccgtacgcgc
     6961 tcatcgctca tcgatggaat gccgaagaaa tcgacgaggt tgagactggc ctgcctgaag
     7021 gtcctcacgg tcaggcgcag cagaacgctg cgaccagcct ccggtcgcat gatcgtcagg
     7081 tttgccatga cacctctgcg ctgccgcgcg ttgaagacga agtagcccag cgcgatagtc
     7141 ttcgcaatgg tgcgcttgag ccaaggaggg agaatcgcag caacgcgaaa ggcgacaaca
     7201 taccagaacg ccatcgccta gcgacccgcc gacccttcga aagctccata ccagagaagt
     7261 ccgataagtg tcttcgcatc gttgatcagc ccgttgcgac accgctccat cgcttcccct
     7321 acaggtatca ggatcggacg aatgaactcg tcagaatcca tcgcggtcgg gcccggttgg
     7381 aggtccctcg cgacgtagag tgtcagccgc tcatcgacaa aaccggggct cggccagaac
     7441 tcgacacact tcttccagga gcccgccgta tatcctgtct cttcacggag ctcacgacgc
     7501 gcagcagtca ggaatcgctc ccgcacaccg cgtcgccccg cagggatctc cagcatgacc
     7561 gtctccggcg caggccggta ttggcggaca aggacaatcc tgttgtcggc cgcgactgcg
     7621 acgacgcaca cggcaccgcc tgcataggtc acgacgtccc tatgcgcaaa gcggtcgtcc
     7681 ggcaatcgga cgaccttgtc caggagttct atggacttcc cgagatacgc ccgtcggcac
     7741 gacacgacgg tctcaaacat ccgctcgcgt gtcctcaatg agatctgatc atctccttga
     7801 tcttcaacag cctcgtcgta ttcgcccgtt cattctcgtc cagtttcgac gtgatcgatc
     7861 gaatcgtctc
//
LOCUS       DATMZD010000090         2221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_123328,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000090 DATMZD010000000
VERSION     DATMZD010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123328"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="VN478_01860"
     CDS             <1..311
                     /locus_tag="VN478_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HWQ21455.1"
                     /translation="FSLGLSWATSGSVPVAGSLLAGLGVGCVSYGVSLVLFILALKAM
                     GAARTGAFFAVGPLAGALLSLVMFRSPFTCAMAVALVLTAASITLVASEGLRRRGTAD
                     "
     gene            382..819
                     /locus_tag="VN478_01865"
     CDS             382..819
                     /locus_tag="VN478_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014011666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HWQ21456.1"
                     /translation="MKWVTRAHVHVDRVACPWLISRFVDSEAEFAYVATDIVDQFAQN
                     EVATPFDLEGVELGHQDGHCSFVSIMEKYHLTDKALVQLADVVNAADTDQLDANPYAR
                     GLEALAQGFSLMYPDDTVSVEAQFAVYDALYAFFRLKVAGEGR"
     gene            902..2188
                     /gene="eno"
                     /locus_tag="VN478_01870"
     CDS             902..2188
                     /gene="eno"
                     /locus_tag="VN478_01870"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012723166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HWQ21457.1"
                     /translation="MTTAIKNVHAREILDSRGNPTVEVEVTLASGIMARAGVPSGAST
                     GIHEALELRDGDKARYGGKGVLKAVEHVNNEIASAVIGKDATDQSGIDHTMIELDGTP
                     HKTKLGANAILGVSLATARVAALACDLPLYRYIGGVTATMLPVPMFNIMNGGVHANWQ
                     GTDLQEFMIAPVGAPTFHEAVRWASETYHTLQGVLKAAGYTTGVGDEGGFAPALKRNA
                     EAIELIIQAIEKAGYKPGEQIAIALDPASSGFYEDGLYNLRTEGRKATSAEMVAMYAD
                     WVEKYPLVVLEDGLAEDDWDGWKLLNQTIGQKIELVGDDLFVTNVERIQRGIEENAAN
                     AVLIKLNQIGSLTETIAAITMARKAGWGAMVSHRSGETVDSFIADLTVGMGTGHLKTG
                     APCRGERVEKYNQLMRIEEELGSQASYAGHGAFVRP"
BASE COUNT          442 a          669 c          712 g          398 t
ORIGIN      
        1 tcttctccct gggactctcg tgggcgacca gtggcagtgt acctgtggcg ggtagccttc
       61 tggccggcct cggcgtcggc tgcgtctcct acggggtatc actcgtactg ttcatccttg
      121 cactcaaagc tatgggggca gcgcgtacgg gagccttctt cgccgtgggt ccgctggctg
      181 gggccctcct gtctctggtc atgtttcgca gccccttcac gtgtgcgatg gcggtggcgc
      241 ttgtcctgac ggcagcgtcg atcacgctgg tggcaagcga gggtttgcgc aggcggggca
      301 cagcagactg acccgttgac cctctgaccg ttccggtcag actgtggtat caggaggaga
      361 tctgtcggcc gggaggctca tatgaagtgg gtaacgcgcg cgcacgtgca tgtggacaga
      421 gtggcttgtc cgtggctgat cagccggttc gtcgacagcg aagcggagtt cgcgtatgtg
      481 gcgaccgaca tcgtcgacca gttcgcacag aacgaggttg cgacgccctt cgacctcgaa
      541 ggcgtcgagc tgggtcatca ggacggtcac tgcagcttcg tctccatcat ggagaagtat
      601 catctcaccg acaaggcgct cgtacagctt gccgacgtcg tcaacgccgc ggatacggat
      661 cagctggacg caaacccgta tgccaggggt ctggaagcgc tggcacaggg gttctccctg
      721 atgtaccctg acgacaccgt cagcgtggag gcgcagttcg ctgtgtatga tgccctgtac
      781 gcgttcttcc ggctcaaggt ggccggcgag ggcaggtaga ctcacacatc actcacactg
      841 gaaatacgga tcggccgaac aatactatgg cgtttacaat gctgcaccag gaggaatatc
      901 aatgacgaca gctatcaaga atgtccatgc acgagagatc ctcgattcac gcggcaatcc
      961 gaccgtcgag gtcgaagtca ccctcgcgtc cggcatcatg gcaagggcag gcgtcccatc
     1021 cggagcgtct accggcatcc atgaagcgct ggaactgcgc gacggagaca aggcccgcta
     1081 tggcgggaaa ggcgttctga aagccgtaga gcatgtgaac aacgagattg catcagcggt
     1141 catcggcaag gacgcgaccg atcagtccgg catcgaccac acgatgatcg agctggacgg
     1201 aacgcctcac aagacgaagc ttggcgccaa cgccattctg ggcgtcagcc tggcgactgc
     1261 gcgggtcgcg gcgctggcct gtgatcttcc gctgtaccgg tacatcggcg gcgtgacggc
     1321 gaccatgctg cccgttccca tgttcaacat catgaatggc ggagtccacg ccaactggca
     1381 ggggaccgac ctgcaggagt tcatgatcgc gcctgtgggc gcacccacgt ttcacgaggc
     1441 cgtacgctgg gcaagcgaga cgtatcatac gctccagggc gtgctcaagg ctgccggcta
     1501 caccaccggc gtgggcgacg agggcggatt cgccccggcg ctcaagcgca acgctgaagc
     1561 catcgagctg atcatccagg ccatcgagaa ggctgggtac aaaccgggcg agcagatcgc
     1621 catcgcgctc gatccagcgt ccagcggctt ctatgaagac ggcctctaca acctgcgcac
     1681 agagggtcgc aaggcgacct ccgcagagat ggtcgccatg tacgccgact gggtggagaa
     1741 gtatcccctc gtcgtccttg aggacggcct ggccgaagac gactgggacg gctggaagct
     1801 gctgaaccag accatcggac agaagatcga gctggtcggc gacgacctgt ttgtcaccaa
     1861 tgtcgagcgc attcagcgcg gcatcgagga gaacgcagcc aacgccgtcc tcatcaagct
     1921 gaaccagatc ggttccctga ccgaaaccat cgcggcgatc acgatggcgc gcaaggcagg
     1981 gtggggcgca atggtctccc accgcagcgg tgagacggtc gacagtttca tcgccgacct
     2041 caccgtcggt atgggcacgg gtcacttgaa gactggtgcc ccctgccgtg gcgaacgggt
     2101 cgagaagtac aaccagctga tgcgcatcga agaggagctg ggcagccagg cttcctacgc
     2161 cggtcacggc gcgttcgtac ggccttaact ggcaccgaag agcatttcaa ctgcgccgcg
     2221 g
//
LOCUS       DATMZD010000091         3841 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_62842,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000091 DATMZD010000000
VERSION     DATMZD010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3841
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62842"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /gene="rho"
                     /locus_tag="VN478_01875"
     CDS             <1..888
                     /gene="rho"
                     /locus_tag="VN478_01875"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013985710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HWQ21458.1"
                     /translation="VLARPQFESLTPIFPNKHLKLETPNCSMALRLLDLVAPIGKGQR
                     GLIVSPPKAGKTTILKNVAQSIEYNHPEVTLMVLLIDERPEEVTDMKQSIRSEVISST
                     FDQPPENHIRVVELALERAKRLVEVKKDVVILMDGITRLTRAYNLISSSSGKTLSGGL
                     DPTAIRGPKRVIGAARNMVEGGSLTIIATALIETGSRLDQVIYEEFKGTGNMELVLDR
                     ELAERRIFPAIDVRKSGTRREELLYTPEEYRKIWALRQFIGNEDPTESLEKLIAMLQR
                     TANNREFLAQIVSQDSTGR"
     gene            894..1502
                     /locus_tag="VN478_01880"
     CDS             894..1502
                     /locus_tag="VN478_01880"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008880714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HWQ21459.1"
                     /translation="MDSCSGRIEVIAGCMFSGKSEELIRRLKRAQIARQKVIALKSSL
                     DDRYGLETITSHSGFRLESAVVACPSDVFRIVEERQVEVVGIDEVQFFDPAIIEVAEY
                     LAGTGVRVILAGLDQDFRGEPFGPMPDLLSLAEEVTKLTAVCMVCGKPATRTQRIVNG
                     RPARYDDPVVMVGAAESYEARCREHHVVPGRPECVPDRERLI"
     gene            1519..2607
                     /gene="prfA"
                     /locus_tag="VN478_01885"
     CDS             1519..2607
                     /gene="prfA"
                     /locus_tag="VN478_01885"
                     /inference="COORDINATES: protein motif:HMM:NF001859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HWQ21460.1"
                     /translation="MSLETEQLARRIHEIDERVASLRYDVDQKEIQKLTLERRAMDSL
                     FGLLTRQESLRASLVDNENLLAGGTDSEFAAVIAEDNLRLHQELEAVHAQIEEERIPK
                     DPDDDKNIIVEIRGGVGGDEAALFAADLFRMYSYFAGQHGLKIEVISSHPTEIGGFKE
                     IVFSVLGTGSYKLFKFESGVHRVQRVPETEASGRIHTSTATVAVLPEAEDVDVHIDDS
                     DIRLELFHASGHGGQNVQKVETAVRMTHIPTGITASCQDERSQLKNKDKALKVLRSRV
                     YEFMKEKADSEMIDERRAQIGSGMRNMRIRTYNFPQGRLTDHRIGLSLYNLPLIMEGN
                     LDPVVDALRRAVRERTLETPILQDSDDD"
     gene            2604..3467
                     /locus_tag="VN478_01890"
     CDS             2604..3467
                     /locus_tag="VN478_01890"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK/PrmC family methyltransferase"
                     /protein_id="HWQ21461.1"
                     /translation="MTLREVLGRAARRALLSPAKDGELQYVVAANRGWSRSDLLIRLD
                     LPVSGGVKESIEHAARRLADGYPAEYLTGRAAFCDVTVRVQEGVLVPRPETESLVQMG
                     EDFVVEERRPSPIALDLCSGSGAIALALAAKLPWVTVVGLDLDPVSIACSRESALDAG
                     LSERVTFIQGNVLADWSLTLSEFRSRADVIISNPPYIREERMLEAHEASPVEPVPALY
                     GGASGMVFYRRIIAQACEWLRPGGLLLLEIDDGLEQGILDLFERHGLLSGRWLPDLRG
                     LPRYAEARRAE"
     gene            3464..>3841
                     /locus_tag="VN478_01895"
     CDS             3464..>3841
                     /locus_tag="VN478_01895"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HWQ21462.1"
                     /translation="MNSFDYIVRDLVVFAVAYLVGSLPFSYIFAKLVKGVDITKVGSR
                     NMGATNVLRTAGPVPAVFAFAGDLLKAILLLLALTRFFPDVSREAVLVAAVAVVLGHD
                     FSVLAGFKGGKGVATTFGVVLMLS"
BASE COUNT          789 a         1048 c         1195 g          809 t
ORIGIN      
        1 gtgcttgctc gaccacaatt cgagtcgctc acgcccatct tcccgaacaa gcacctgaag
       61 ctggagacac cgaactgcag catggcattg cgtctgctcg atcttgtcgc tcccatcggc
      121 aaggggcagc gcggccttat cgtctctcct ccgaaggctg ggaagaccac gattctcaag
      181 aacgttgctc agagcataga gtacaatcac cctgaggtca ccctgatggt actccttatc
      241 gatgagcggc ccgaagaagt cacggacatg aagcagtcga tccgcagcga agtcatcagc
      301 tcgacgttcg accagcctcc tgagaaccac attcgggtcg ttgagttggc actggaacgc
      361 gcgaagcgtc tggtcgaggt taagaaggat gtcgtcatcc tcatggatgg catcactcgg
      421 ctgacacggg cctataacct catcagttcc tcgtctggga agacgctgtc gggcggtctc
      481 gacccgaccg caattcgcgg cccgaagaga gtcattggag ccgctcggaa catggtcgag
      541 ggcggcagtc tcaccataat cgcgactgcg ctgatcgaga ctggaagtcg cctggatcag
      601 gtcatctatg aggaattcaa gggcacaggc aacatggaac tcgttctgga tcgcgagctg
      661 gccgagcgtc gcatcttccc tgcaattgat gtgcggaagt ccggcacaag acgcgaggaa
      721 ctgctctaca cgcctgagga atatcgcaag atctgggccc tcagacagtt catcggcaac
      781 gaggacccaa ctgagagtct ggagaagctt attgccatgc ttcagaggac cgcgaacaac
      841 agggagttcc tggcccagat cgtgtcacag gacagcactg gacgctagtc gctatggata
      901 gctgttccgg caggatcgag gtcattgccg gatgcatgtt ctcggggaag tccgaggaac
      961 tcattcggcg tctcaaaagg gctcagatcg cgagacagaa ggtcattgcc ctcaaatcgt
     1021 ctcttgatga tcgctatggg ctcgagacaa taacgagtca ttcgggattt cgcctggagt
     1081 cagctgttgt agcatgcccg tctgacgtgt tcaggatcgt ggaggagagg caagtggaag
     1141 ttgttggcat tgacgaagta cagttcttcg atcctgccat catcgaagta gcggagtatc
     1201 ttgcaggcac aggcgtccgg gtcatccttg ccgggctgga tcaggacttt cgtggtgaac
     1261 cgttcggacc catgcctgac cttctgtcgc ttgcggagga agtgacgaag ctgacggctg
     1321 tgtgcatggt ttgcggaaag ccggcgacac gtacccagcg catcgtcaat gggcgtccgg
     1381 cgcgctatga cgaccctgtc gtgatggtgg gcgcagccga gagttatgag gcgcggtgcc
     1441 gtgagcatca cgttgttccc ggtcgtccag agtgtgttcc ggacagggag cgcctcatct
     1501 gaggcacaga gagagctgat gagcctagag acagaacagt tggcgcgcag aatccatgag
     1561 atcgacgagc gagtcgccag cctccgctat gacgtcgacc agaaagaaat ccagaagttg
     1621 accctggaac ggcgggccat ggactcgctg ttcggactac tgactcggca agagtcgctc
     1681 agagcatctc tcgtcgacaa tgagaacctt ctcgcaggag gaacggattc cgagttcgct
     1741 gcggtgatcg cagaagacaa tcttcgactg catcaggagc tcgaggccgt tcacgcacag
     1801 attgaggagg aacgtattcc caaggaccca gatgatgaca agaacatcat cgtcgagatc
     1861 cgtggaggcg tcgggggcga tgaggcggca ttgttcgcag ctgatctgtt ccgcatgtac
     1921 tcctacttcg ctggtcagca cggcctcaag atcgaggtca tctcgagtca ccctactgag
     1981 atcggtggct tcaaggaaat cgtcttctca gtgcttggaa cgggaagcta caaactgttc
     2041 aaatttgaga gtggcgttca ccgagttcag cgcgtccccg agaccgaagc gtctggacgc
     2101 atccacacgt ctactgctac tgtggctgtg ctacctgaag ccgaggacgt cgacgtgcac
     2161 atcgacgact ccgacatcag acttgagctg tttcatgcct ccgggcatgg gggacagaac
     2221 gtccagaagg tggagacggc agtgcggatg acgcacattc ctacgggcat taccgcgtca
     2281 tgccaggatg agaggtctca gctgaagaac aaggacaaag cacttaaggt cctccggtct
     2341 cgcgtctacg agttcatgaa ggagaaggcg gacagcgaga tgatcgatga gcgcagagcc
     2401 cagattggca gcggcatgcg caacatgcgt atccgcacat acaacttccc acagggtcgt
     2461 ctgactgacc atcggatcgg cctgtcgctg tacaaccttc cgctcattat ggaagggaat
     2521 cttgacccag tggtggacgc tctccgtcga gctgtccggg agaggaccct ggagacgccc
     2581 atcttgcagg acagcgacga cgattgactc tccgagaagt acttgggcgg gctgcgcggc
     2641 gcgcgttgct gtctccggcg aaggatggtg aactgcaata tgtcgttgcc gccaacagag
     2701 gctggagccg ctcagatctg ctgatccggc ttgatcttcc ggtcagtggt ggcgtgaagg
     2761 agtccattga gcatgcagcg cggcgcctgg ccgatggata ccccgcggag tacctcactg
     2821 gtcgcgcagc cttctgcgat gtcaccgtac gggtgcagga aggcgttctc gtgcctcgcc
     2881 cggagacaga gagcctcgta cagatgggag aggatttcgt tgtcgaggaa cgtcgaccca
     2941 gccctattgc tctggacctg tgttctggtt cgggagccat agcactggca ctggctgcca
     3001 agctgccgtg ggtgacggtc gtcggcttgg atcttgaccc tgtctccatc gcatgttcgc
     3061 gggagtctgc cctggacgcc gggctgtcgg aacgagtcac cttcatacag ggcaatgtgc
     3121 tggcagactg gagtctgact ctctccgagt ttcggagtcg cgctgatgtc atcatcagca
     3181 atccgcccta tatacgcgaa gagcgcatgc tcgaggctca cgaggcctcg cctgttgaac
     3241 cagttccagc tctctatggc ggcgcatcag gcatggtctt ctacaggcgg atcattgctc
     3301 aggcttgcga gtggttgcgg cccggaggtc tgctgctcct ggagattgac gatgggctgg
     3361 aacagggtat tctggacctg tttgagcggc atggattgct gagcgggcga tggttgcccg
     3421 acctcagggg gctgccgcgc tatgcggagg cacgtcgagc agaatgaact cattcgacta
     3481 tatcgtgcgc gatctggtgg tctttgccgt tgcctatctt gtgggctccc tgcccttcag
     3541 ttacatcttc gccaagctgg tcaagggtgt cgacataacg aaggtgggca gccggaacat
     3601 gggtgccaca aacgtgctgc gcactgcggg ccctgtccct gcggtgttcg cttttgccgg
     3661 ggacctgctc aaggcgatac tccttctcct ggcgctgacg aggttcttcc cggatgtatc
     3721 gcgtgaagcg gtgcttgtgg cggcggtcgc cgtggttctg ggccacgact tctcagtgct
     3781 ggccggcttc aaggggggca agggcgtggc tacgactttc ggagtcgtgc tgatgttgag
     3841 c
//
LOCUS       DATMZD010000092         2068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_32945,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000092 DATMZD010000000
VERSION     DATMZD010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32945"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="VN478_01900"
     CDS             <1..535
                     /locus_tag="VN478_01900"
                     /inference="COORDINATES: protein motif:HMM:NF015129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21463.1"
                     /translation="IESILLELHVPCTVASLDTTLLETGASALVSSDSTELGTLGILR
                     HNIGALLGLDRETYYAEFSVDALLKSIPAIAFKEFSKFPSVRRDIAVLVDNDMTAGRL
                     QAIIAENAGPLLKAIVVFDSFQGGSLPSGKKSVGFSMEFSSPDRTLLGEEVDILVSSV
                     EAALATRVGGILRRTRV"
     gene            760..>2068
                     /locus_tag="VN478_01905"
     CDS             760..>2068
                     /locus_tag="VN478_01905"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HWQ21464.1"
                     /translation="MRAELDKAKVRLQLAGQEVWRVCVVLNELSRVRKAFVTIKDLYP
                     ALFSISQDVKPFSSLAQEIERCVNQDGDVLDDASINIAAIRREKIELQKTINKVLQDI
                     LGSETYGRAVQDRIVTMRNDRYVIPVKREFKDTIQSVVHDQSDSGMTLFVEPTRVIDL
                     NNRLQILQSDEKKEISRILLYLTDLIAKAAPDIERSLTVAGHLDFSLAKGRLAKSWNG
                     VKPSVSPNLETNLRNVRNPFIADCVPVSFSVGKDYFMVVITGPNTGGKTVALKTLGLT
                     ILISKAGLFISASEGCTVGLYDEVFVDIGDEQSIEQSLSTFSAHMSNIVRILAQATRY
                     SMVLLDELGVGTDPEEGSAIATGIVDFLYQRRVSTVITTHYSSLKLLAYRYDQIRNAS
                     VGFDVETLRPTYELIIGSPGESHAFTICERLGLPEAVLALAHDE"
BASE COUNT          440 a          615 c          584 g          429 t
ORIGIN      
        1 catcgagtct atccttctcg aactgcatgt tccgtgcact gttgcgtcac ttgacactac
       61 tctcctggag accggcgcaa gtgcactggt ctcctcggat tccactgagc taggcacgct
      121 tggcattctc aggcacaaca tcggcgcgct actgggcctg gaccgcgaaa cgtactacgc
      181 cgagttctct gtagatgcgc tgttgaagag tattccagct atcgctttca aggagttctc
      241 caagttcccg tcggtccggc gtgacattgc tgtcctggtt gacaatgaca tgacggccgg
      301 ccgactgcag gcgatcatcg ccgaaaacgc cggccccctt ctcaaggcaa ttgtcgtgtt
      361 cgacagtttc caggggggca gtctaccgtc aggcaagaag agcgtgggct tctcgatgga
      421 gttctcctca cccgatcgga cactgttggg cgaggaagtc gatatccttg tcagctccgt
      481 cgaggccgcc cttgcaactc gtgtgggggg catactacga agaacccgag tctagaggat
      541 cagcaggctg ctcgcgaata cacgtacggc aaggtcggct tcgatcgcat ccaggaggct
      601 ctggcttcac aggcagagtc cgctcttggt gcggaagagg ctcgccgact cgctccactc
      661 gacagcatcg acagtatcag ggcggcccag acggagactg acgaggcctc ttcatacctg
      721 gagaggttcg gcgacctgac cctcggcaat ctcctcgatg tgagggccga actggacaag
      781 gccaaggttc gtctgcagct ggctgggcaa gaagtctgga gagtctgtgt cgtcctgaac
      841 gagctttcac gtgtccgcaa ggcatttgtg acgatcaaag acctctatcc ggcccttttc
      901 agcatttcgc aggatgtcaa gccgttttcg tccctggctc aggagatcga acgctgtgtc
      961 aatcaggatg gcgacgttct cgacgacgca tcgatcaaca tcgcagccat tcgtcgcgag
     1021 aagatcgagc tgcagaagac catcaacaag gtgctgcagg acattctcgg gagcgagacc
     1081 tatggacgcg ccgttcaaga ccgcatcgtc acgatgcgga acgatcgcta tgtgatcccg
     1141 gtgaagcgtg agttcaagga taccatccag tcggttgtcc atgaccagtc tgacagcggc
     1201 atgaccctgt tcgtcgaacc cacgcgcgtg atcgacctga acaaccggct gcagatcctg
     1261 caatccgacg agaagaagga gatatcccgt attctcctct acctgacgga cctgatcgca
     1321 aaggccgctc cggatatcga gaggtcgctg accgtggccg gacatctcga cttcagcctg
     1381 gcaaaagggc gcctggccaa atcctggaac ggcgtcaagc cgtcggtcag ccccaacctg
     1441 gagaccaatc tgcggaatgt ccgcaaccca ttcatcgcgg actgcgtccc tgtgtcgttc
     1501 tctgttggca aggactactt catggtggtc atcaccggtc cgaatacagg tggaaagacg
     1561 gtggccctga agaccctcgg cctcaccata ctcattagca aagcgggact gttcatctca
     1621 gcttcagaag ggtgtacggt cggactctac gacgaggtct tcgttgacat tggcgacgag
     1681 cagagtatcg agcagagtct gtcgacgttc tcggcacaca tgtcgaacat cgtgcgcatc
     1741 ctggcacagg cgacccggta ctcgatggtc ctgctggatg aactcggtgt cggtacagac
     1801 cccgaagaag gctccgcgat cgcgacaggc atcgtcgact tcctctacca gagacgagtt
     1861 tccacagtca tcacgacgca ctatagcagc ctgaagctcc tcgcataccg ctacgaccag
     1921 atacgaaacg cttctgtggg tttcgacgtc gaaaccctgc ggccgaccta cgagctgatt
     1981 atcggctccc cgggcgagag ccatgcgttc actatttgcg agagactcgg gctccccgag
     2041 gctgtgctcg cacttgccca cgatgaac
//
LOCUS       DATMZD010000093         8711 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_53093,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000093 DATMZD010000000
VERSION     DATMZD010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8711
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53093"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1281
                     /locus_tag="VN478_01910"
     CDS             <1..1281
                     /locus_tag="VN478_01910"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HWQ21465.1"
                     /translation="VDDNVIAAIGTGVPVLASPADRVVSGARMLMMPGFVNTHHHLYQ
                     TLFRNVPQVEHAKLFDWLTFLYEHWKYIDEEAVYSSAQVGICEMLKTGVTTTTDHLYL
                     YPYGNNTIIDAEIRAARDIGVRFHPTRGSMSLSKKDGGLPPDSVVQTDEEILVESERV
                     IHQYHDRGRYAMTRIALAPCSPFSVTTELMKQTAALAEKEDVLVHTHLAETQDEEEFC
                     LQTFGFRPVDYMEEVNWLTPRSWFAHIVWASDVDIHKLSEHKCGMAHCPSSNMRLGSG
                     IAPVSIMKQTDMRIGIGVDGSASNDSNSFIGEVRLAMLLQRVKYGAEALTSREALAMG
                     SMGGASVLRMESDIGSIEVGKAADLIMWNLDTLEFAGGLHDPFTAPVMCDAKQVSFSM
                     VNGNVLIENGQFTNVDVQALVAKQNTIAAKLMAR"
     gene            1332..2765
                     /gene="glnA"
                     /locus_tag="VN478_01915"
     CDS             1332..2765
                     /gene="glnA"
                     /locus_tag="VN478_01915"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HWQ21466.1"
                     /translation="MKTLQQFLDYAKGHEAVQVDLKFTDLFGGLHHVTVPLSRVDQGF
                     LTQGVAFDGSSIPGFASVERSDMLLRLDLSTAFVDPFAKVPTVAVFCDILEPHTHQRF
                     ALDPRFIAEQAEALLKSKGYADTAWFAPEFEFYVFQGVDFKSSETTSFFSIQSVETGS
                     TVSLDNSQLQGYSIRHGQGYHTEQPLDRLFDYRSTLVRMLEDAGVAIRYHHHEAGETG
                     QIELEVMPCTPRAMGDSAQKIKYFAKNLARQMGMTATFMPKPAAGQAGTGMHFHQYLT
                     RKGQPTFYQKGGEPYDLSPLAVHYIGGILSNASALLGITNPSTISYRRLIPGFEAPIR
                     AFFSLGNRTAAVRIPIYADTAAEKRIEFRPPDATTNVYLAISAMLLAGLDGIEKETDP
                     TALHLGPYTGDIEKLSEKVVRKIPVLPTSLSEAFAALRHGSGFLTRDGVFPPGFVEAF
                     CSYKERNEAEVLRRLPHPKEFELYYDC"
     gene            2775..2999
                     /locus_tag="VN478_01920"
     CDS             2775..2999
                     /locus_tag="VN478_01920"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HWQ21467.1"
                     /translation="MLINLKLHAIIADQVGDLHDVQVPAGTDIAGLLAALRIDRDRAG
                     FVLVNGQRAPYDTELHDKDTVFIIPFLGGG"
     gene            complement(3501..3872)
                     /locus_tag="VN478_01925"
     CDS             complement(3501..3872)
                     /locus_tag="VN478_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21468.1"
                     /translation="MYLLVIITRQEDKTYDFLKLLPTVGVRGATVIEGRGMGKILRDR
                     EVVFTSIEQVLSASIDLTDNVVILSLVQGGDVLASARRLARQVFGDFSHPNTGVLFVL
                     PVADAEGLATGPDATLPEVSR"
     gene            complement(4003..4782)
                     /gene="udp"
                     /locus_tag="VN478_01930"
     CDS             complement(4003..4782)
                     /gene="udp"
                     /locus_tag="VN478_01930"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006570632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HWQ21469.1"
                     /translation="MAHTMFNGEYKYHIRLKQGDVGKYVLLPGDPGRCEVIAKWFDDP
                     VKVAQNREYVTYTGTLLGERVSVTSTGIGGPSTAIAVEELAMVGADTFIRVGTSGAMQ
                     PDINVGDLAIVTAAIRDEGTTRQYMPIEFPAVANIDVTLALRTAAERLHLRSHLGISQ
                     SKDSFYGEVEPGRQPLSEQLNARRRAWIAGGAVCSEMEAATVFVVSSILRRRAGGVML
                     IVDSAFVEGGDEPHHEALDRLISTGIEATKVLIEHDRAVRV"
     gene            complement(4817..5380)
                     /locus_tag="VN478_01935"
     CDS             complement(4817..5380)
                     /locus_tag="VN478_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015218925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HWQ21470.1"
                     /translation="MGFWTVFIIAVGLSMDAFAVSVASGCRIKHVSPSDALRPGVWFG
                     GFQALMPVLGWFGGLTLRNLIQDFSHWIAFALLVFVGGKMLVEAVRGREREEECLEER
                     RSTRDMLVLAIATSIDALAVGLSLSVLRVSIWWPALLIGLVTCAFSIVGTLLGCTVGR
                     LVKDKAEILGGLVLIGIGIRILVQGLR"
     gene            5584..6255
                     /locus_tag="VN478_01940"
     CDS             5584..6255
                     /locus_tag="VN478_01940"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HWQ21471.1"
                     /translation="MNIGLKYGALIIASYLWGSVCWGLVLSFVVKHEDIRLKDNPGLS
                     GSVRQYGWAYGLTVGLLDVMKGYVLGLFLRYMDVPSWVLIVSFMAVIVGHNWPIFFQF
                     RGGGGIATTLGILLQGYLTAVLWGLPFAAVAGVIWKLVPRLKAKVHFSPFISAVGAVP
                     IVVWIVMHRPWYPDYIIVVALAASVLIKGNQFHIQAQRLRTYAYGMRDRFMDHYRDNN
                     PPPTP"
     gene            complement(6315..7427)
                     /locus_tag="VN478_01945"
     CDS             complement(6315..7427)
                     /locus_tag="VN478_01945"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWQ21472.1"
                     /translation="MIRIAARHLFDGHRIMTDAIVTIDGPRVVSVETPDRNGGFCDYS
                     AAWVTPGLIDLDSGIGLKEESLGFEGNDLNEATEAVTPEMQALDGLSPYDTSLGKSLM
                     GGVTAALVMPGGSNVVGGRGAVVHMAGATADGMCIKAPFGTKFSLGSVPKQTHGQKRT
                     PMTRMGNAFLIRDVLTKARDHREKKKEYNMKYEALLPLLAGEDVAFIQALRADDIMTA
                     VRLAEEFGLRYVVTGAFDADLVADQLKAKDVAVAFGPVIMSRSTDEIRRLHPENAVAL
                     LDTGVRTAIISGHPSYPAKYLRVSLGLLVGRRVSPEQALGSVTSVPARLLGLDGYGEL
                     QPGSVADLAMFRAEPWEPEGITERTFISGSEVYARS"
     gene            complement(7424..8578)
                     /locus_tag="VN478_01950"
     CDS             complement(7424..8578)
                     /locus_tag="VN478_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HWQ21473.1"
                     /translation="MILIHGGTILTVTDGVIEGGSVLLGDDGKIAEVFTSHTDVVGAT
                     VIDAAGKYVVPGFVDAHTHLGICNLEVGEIGEDENEMTTPSTPACRALDAVYVWDSGF
                     TDALSGGVTTVFTGPGSGNVIGGQSITMKTGGHCIDEMVVQSPAGLKTASGENPKRVY
                     KGKNQLPSTRMGTAKVLREALSKARDYRERRAGAEPTATDLDMEVLCKVLEREIPLRT
                     HSHRADDMMTMVRIRDEFGVRMVIEHGTDSARIRHELAKAGVAVIAGPLLDVSPKVET
                     ASASFSTPHLLHEAGVLTAIMTDHPVIPIESLRLEAAIAAYEGGAGEQEALEFITVNP
                     AEILGLVDRIGSITKGKDADVVVWSGHPFKASSIAEKTFVNGKLAWEVNA"
BASE COUNT         1770 a         2749 c         2428 g         1764 t
ORIGIN      
        1 gtcgacgaca acgtcatcgc agccataggc acaggagtcc cggtccttgc ctcgcctgca
       61 gatcgtgtcg tctccggcgc gcgcatgctg atgatgcctg gctttgtcaa cacccaccac
      121 cacctgtacc agacgctgtt cagaaacgtt ccccaagtcg agcatgccaa gcttttcgac
      181 tggctgacgt tcctctacga acactggaag tacattgacg aagaggctgt ctatagtagt
      241 gcccaggtcg gcatctgcga gatgctgaag accggcgtta cgacgaccac cgaccatctc
      301 tatctatatc cgtacggcaa caataccatc atcgatgcag agatccgcgc agcccgtgac
      361 atcggcgtac ggttccatcc gacgcgtggc agcatgtcct tgagcaagaa ggacggcgga
      421 ctccctcccg acagcgttgt gcagaccgat gaagagatcc tggtggagtc tgaacgcgtc
      481 atccaccagt accatgaccg cggccgatat gcgatgacgc gcatcgctct ggcaccctgt
      541 tcccccttct ccgtcacaac agagctgatg aaacagacgg cggctctcgc tgagaaagag
      601 gacgtgcttg tccacacgca tcttgccgag acacaggatg aggaggaatt ctgtctccaa
      661 acgtttggtt tccgtccggt cgactacatg gaagaggtca attggctgac tccccgctcc
      721 tggttcgccc acatcgtatg ggcgtccgac gtcgacatcc acaagctgtc cgaacacaag
      781 tgcggcatgg ctcactgccc atcaagcaac atgcgccttg gttccggcat cgcccccgtc
      841 tccatcatga agcagacaga catgcggatc gggatcggcg tggacggcag tgccagcaac
      901 gacagcaact cgttcattgg cgaggtgcgc cttgcgatgc tgctccagcg cgtgaagtac
      961 ggcgccgagg ctctcacgtc ccgggaagct ctggcgatgg gcagcatggg tggcgccagc
     1021 gtgttgcgca tggaatcaga tatcggcagc atcgaggtcg gcaaggccgc ggacctgatc
     1081 atgtggaacc tcgacactct cgagttcgcc ggcggcctgc acgacccctt cactgcacca
     1141 gtcatgtgcg atgccaagca ggtcagtttt agcatggtca atggcaacgt cctcatcgag
     1201 aacggccagt tcacgaacgt cgacgtccag gccctcgtgg ccaagcagaa tacgatagcg
     1261 gcgaagctca tggcgcggta gcaccacgcc gcggaccagg ttcaggcagc agtttcgcgg
     1321 gaggcaggga catgaagacg cttcagcagt ttctagacta tgctaagggc catgaggccg
     1381 tgcaggtcga cctgaaattc acggacctgt ttggcggcct gcaccatgtg acagttccgc
     1441 tgtcccgtgt cgaccagggg ttcctgaccc agggcgttgc cttcgacggt tcgagcattc
     1501 cggggttcgc gtcggtcgag cgcagtgata tgctcctgcg tctcgatctg tcgacggcgt
     1561 tcgtggaccc ctttgccaag gtcccgaccg tcgcggtctt ctgcgacatc ctcgagcccc
     1621 acacacatca gcgttttgct cttgaccctc ggttcattgc agaacaggct gaggcgctgc
     1681 tgaagtccaa ggggtatgcc gacacagcct ggttcgcccc cgaattcgag ttctacgtct
     1741 tccagggtgt cgacttcaag tcctccgaaa ccacatcttt cttctcgatc cagagtgtgg
     1801 aaacgggaag cacggtgtcg ctcgacaact ctcagctcca ggggtactcc attcgccacg
     1861 gacaaggcta ccatactgag caaccactcg atcgcctctt cgactatcga agcacgctcg
     1921 tccggatgct ggaagacgcc ggcgttgcga tccgctatca ccatcacgag gccggtgaga
     1981 cagggcagat agagctcgag gtcatgccat gcactccacg tgccatgggc gactcggcgc
     2041 agaagatcaa gtacttcgca aagaaccttg cccgtcagat gggcatgaca gcaaccttca
     2101 tgcccaagcc ggccgccggc caggccggaa ccggaatgca cttccaccag tatctgacgc
     2161 gaaaggggca acccacgttc taccagaagg gtggagagcc gtatgatctg tcgcccctcg
     2221 ccgtccacta cattggaggg attctcagca acgcctcggc acttctgggt atcacaaacc
     2281 cgtccaccat cagctacagg aggctcattc cgggatttga ggccccgatc cgggcgttct
     2341 tctctctggg caaccgtacc gctgccgttc gcatccccat ctatgcggac acggcggcgg
     2401 agaaacgcat cgagttccgc cctccggacg ccaccaccaa tgtctacctg gcaatcagcg
     2461 cgatgctgct cgccggactc gacggcatcg agaaggagac ggatccgaca gcactccatc
     2521 tcggaccgta cacaggagac atcgagaagc tgtccgagaa ggtggtccgc aagattcctg
     2581 tgcttccgac ctcgctttcc gaagccttcg ccgccctgag acacggcagc ggtttcctga
     2641 cgcgagacgg cgtcttccct cctgggtttg tcgaggcatt ctgctcgtac aaggagagga
     2701 acgaggctga agtacttcgc cgtctgcctc atcccaaaga gttcgagctc tactacgact
     2761 gctgaggacg tcggatgctc atcaacctca agctgcacgc cataattgcc gatcaggtgg
     2821 gagatctgca tgatgtgcag gtgccagccg gaacggacat tgccggcctg cttgcggcac
     2881 tgcggattga ccgcgaccgt gcaggcttcg tgctcgtcaa tggacagcga gcgccctatg
     2941 acacggagct tcacgacaag gacacggtct tcatcatccc ctttctggga ggtggatgac
     3001 cgcggcctgt catgttcatg aacggaagag acccccagac tcacgggggc ctctggttat
     3061 gtgcgcgcca tagaactgct atatgtcctt gatgatgatg ttgcctacga tgtctgccgc
     3121 gtcagcaatc ctgtcagcag agttacagtg accgtcccct agaatatttc catccgaatc
     3181 gaagacaggg gcacgatacg gctgccaagt gtcgcagcaa cggcggtccc gatgacaagg
     3241 ggtgcgagca cgatggaagc gaacagaaga atgacagccc acgccggtcg tatgaaattg
     3301 gtggccacga tggagccgag gagcgttcgc ccatcgttcg cgcctataag cagttgcagg
     3361 gccagcgcag cgaggacaat gcgtaatact caacggtcac cgtggtgttg tcctctcgga
     3421 gtattgctca attcgacatc tctttcgcga cgtcatgcgg cgtgcagaca tctggatcgc
     3481 gcacggcgaa cacatgttgt ctatcggctc acttccggga gtgtcgcatc cggccctgtc
     3541 gcaaggccct cggcgtcggc gaccggcagt acgaacagga cgcccgtgtt cgggtgcgag
     3601 aagtctccga acacctggcg cgccagtctg cgtgcacttg ccaagacgtc tccaccttgg
     3661 acgagtgaca ggatgacgac gttgtccgtg aggtcgatac tggcactcag cacctgctcg
     3721 atcgacgtga acacgacttc gcgatcgcga aggatcttgc ccatgcctcg accctcgatg
     3781 accgtcgctc ctcgcactcc gaccgtcgga agcaatttca ggaagtcgta ggtcttgtcc
     3841 tcctgtcgcg tgatgatgac caacagatac atgggctccc tccctgacta ctgccaatcg
     3901 catccatcgt cccaacgagg aaggaccatg gattggactc ccgagcgaac accagtgcta
     3961 gagtcaagca cttggcactg cgctcccatg acaccgatct gactagacac ggacggcgcg
     4021 atcgtgctct atgagtacct tggtcgcctc gataccggtc gatatgagcc tgtccagtgc
     4081 ctcatgatgt ggctcgtctc ccccctccac aaacgcgctg tcgacgatga gcatcacacc
     4141 ccctgctcgt ctgcgaagaa tgctggacac cacaaacacg gtagcagcct ccatctcgga
     4201 gcagacagcg ccaccagcga tccaggcccg acgccgcgcg ttcaactgtt cggaaagcgg
     4261 ctggcgaccc ggctcaacct cgccgtagaa tgagtccttc gattgagaga ttccgagatg
     4321 ggacctgaga tggagtctct cagctgccgt tctcagtgcc agtgtcacgt cgatattggc
     4381 caccgccggg aactctatgg gcatgtactg tctggtcgtt ccctcgtccc ggattgccgc
     4441 tgtcacgatc gccaagtctc caacgttgat gtcgggctgc atcgccccgg acgtcccgac
     4501 tcggatgaat gtgtcggcac caaccatcgc cagctcctca accgcgatcg cggtcgaggg
     4561 gcctccgatt ccggtcgagg tgactgacac cctctcaccg aggagtgtcc cggtgtaggt
     4621 gacgtactcc ctgttctgtg ctaccttcac cgggtcatcg aaccactttg cgatgacctc
     4681 acagcgacct gggtcgccag gcagcagaac gtacttcccg acgtcaccct gcttgagtct
     4741 gatatggtac ttgtactccc cattgaacat cgtatgtgcc atgactgagc ctccgttcct
     4801 tccaccgagg ctacctctag cgcagcccct gtacaagtat cctgatccca atgccaataa
     4861 gaacaagtcc acccaggatc tcggccttgt ccttgacgag tcttccaaca gtgcagccca
     4921 gaagagttcc cacaattgag aacgcgcatg tcaccaggcc gatcagcaga gccggccacc
     4981 agattgagac acgcagaacg gacaggctca acccgaccgc cagagcgtcg atgctcgtgg
     5041 caatcgccag gacgagcatg tcgcgcgtac tccgtctctc ctcgaggcat tcttcctctc
     5101 tctctcgacc gcgcacagcc tctacgagca tcttccctcc gacgaacaca aggagagcga
     5161 acgcgatcca gtggctgaag tcctggatga gatttcggag agtgaggcct ccgaaccatc
     5221 ccagcacggg catgagagcc tggaacccgc cgaaccacac accgggccgc aaagcatcac
     5281 tgggactcac gtgcttgatg cgacagccac ttgccacgga tacggcgaat gcgtccatcg
     5341 agagtcccac cgctatgatg aagacagtcc agaaacccat gcgacctcca cgacaacttc
     5401 gacgtcaccg aaaacgtggc agaaagcgat ggttcgcgct gaacatgcgc ggaacaccgt
     5461 ccgaaaagta tacggccaag atctgccttt acaagccaaa gcattgatat agactgggat
     5521 tgcgactggg cgcaggtcac gtgtggcgca cgaagacgtt gagtggtggt cgggaggatc
     5581 ctgatgaaca tcgggctcaa gtacggcgca ttgatcatag catcgtacct gtggggttct
     5641 gtctgctggg gcctcgtcct cagtttcgtt gtcaagcacg aagacatccg gctcaaggat
     5701 aaccccggcc tttcggggag tgtccgccaa tacggctggg cgtatggcct tacggtcggg
     5761 ctgctcgacg tgatgaaagg ctatgtgctg ggactgttcc tgcgctacat ggatgtcccc
     5821 tcatgggtcc tgatcgtctc attcatggcc gtcatcgtcg gtcacaactg gccgatcttc
     5881 ttccagttca ggggcggcgg cggcatcgcc acaaccctcg gaatcctgct tcagggctac
     5941 ttgacggcgg tcctgtgggg actccccttt gcggcggtag ccggcgtcat ctggaagctt
     6001 gtcccacgcc tcaaggccaa ggtgcatttc tctcccttca tctcagcagt gggagccgtc
     6061 cctatcgtcg tctggatcgt catgcataga ccctggtatc cggactacat tatcgtcgtg
     6121 gcgcttgctg cctcagtcct catcaagggc aatcagttcc acatccaggc acagcggttg
     6181 cgcacatacg catatggcat gcgcgaccgt ttcatggacc actatcgaga caacaacccg
     6241 ccaccgacgc cctagagtcg ctggcagctc gcaggaggga gcggcgcccc tttctgggcg
     6301 ccgctcctga aatcttagga acgggcgtac acctcggacc cactgatgaa cgtgcgttca
     6361 gtgatgcctt cgggctccca tggctctgcc ctgaacatcg ccagatcggc gacagatcca
     6421 ggttgcagct cgccataccc gtccagtccc agcaggcggg caggaacgct tgtcacagat
     6481 ccaagcgcct gttcgggaga gacacgccga ccgaccagca gtccgagcga gaccctcaga
     6541 tacttggctg ggtagctggg gtgaccggag atgatggcag tccggactcc cgtgtcaagg
     6601 agggcgacgg cgttttccgg gtgcaagcgc ctgatctcat ccgtcgatct tgacatgatg
     6661 actgggccga aggctacggc tacatccttc gccttgagct ggtcggccac gagatccgcg
     6721 tcaaatgccc cagtgacaac ataccggaga ccaaactcct cagcgagtcg cacggccgtc
     6781 atgatgtcat ccgcccgcaa tgcctggata aacgccacat cttcgccggc aagaagcggg
     6841 aggagggcct catacttcat gttgtactcc ttcttcttct ccctgtggtc acgcgccttc
     6901 gtgaggacgt cccggatcag gaacgcattt cccatgcgtg tcatgggcgt ccgtttctgg
     6961 ccgtgcgtct gctttgggac gctgccaagg gagaacttcg tgccgaatgg agccttgatg
     7021 cacataccgt cggctgtggc accagccatg tgcacgactg ctcctcgacc tcccacgaca
     7081 ttgctgcctc cgggcatgac cagcgctgcc gtcacgcctc ccatgagact cttgccgaga
     7141 ctcgtgtcat aggggctcaa cccatccagt gcctgcatct cgggggtcac agcctctgtc
     7201 gcttcgttga ggtcgttccc ttcgaagccc agcgactcct ctttgagacc gataccggag
     7261 tcgaggtcga tcaggccagg cgtcacccag gcggccgagt agtcgcagaa cccgccgttt
     7321 cgatccggcg tctcgaccga gaccacgcgt ggcccgtcga tcgtgacgat ggcatcggtc
     7381 atgatccggt gtccgtcaaa cagatgtcgg gctgctatcc tgatcatgcg ttcacctccc
     7441 aggcgagctt gccattcacg aacgtcttct ccgcgatact gctcgccttg aacggatgac
     7501 cactccacac gacgacatcc gcgtccttcc ctttcgtgat gctgccgatg cgatccacga
     7561 gcccaaggat ctctgcggga ttgaccgtta tgaactccag agcctcctgt tctccggcac
     7621 cgccctcata tgccgcgatg gcagcctcca gccggagaga ttcgatcggg ataacggggt
     7681 ggtccgtcat gatggcggtc aacacgcccg cctcatggag aagatgtggt gtactgaacg
     7741 aggccgacgc ggtctcaacc tttggcgaga cgtctaggag cgggcctgcg ataaccgcta
     7801 ctcccgcctt ggcaagctca tgtcgaattc gtgcgctgtc cgttccgtgt tcgatgacca
     7861 tccggacacc aaactcgtca cggatgcgca ccattgtcat catgtcgtca gcacggtgtg
     7921 agtgcgttct caacggaatc tcgcgctcga ggaccttgca cagcacctcc atatcgaggt
     7981 ccgtagcggt gggctcggcg ccggcacgac gctccctgta gtcacgtgcc ttcgacagcg
     8041 cctcacggag aaccttggcg gtgcccatcc gtgtgctcgg cagctggttc ttgcccttgt
     8101 atacacgctt tggattctct cccgatgccg tcttgagccc cgcagggctc tgtactacca
     8161 tctcatcgat gcagtgaccg cccgtcttca tcgtgatgct ctgtccacca atgacgttgc
     8221 cactgccggg accggtgaat acggtcgtaa cgccacccga gagagcatca gtgaacccgg
     8281 agtcccagac gtagactgca tccagagcgc gacacgcagg cgtcgaagga gtggtcatct
     8341 cgttctcgtc ctctccaatc tcgccgacct ccagattgca gatgcctaga tgcgtatggg
     8401 cgtcgacaaa gccgggcacc acgtatttcc ctgccgcatc gatgacggtc gccccgacga
     8461 cgtcggtgtg actcgtgaag acctcggcta tcttgccgtc gtcaccaagc agcaccgaac
     8521 cgccctcgat gacaccgtcc gtgacagtga ggatcgttcc tccgtgtatg agaatcatac
     8581 gactcctttc ctgaaggggc aaagcagcac ctccttcggt cttttcttcg gtaagcgaat
     8641 tatagttgag tagccggaca gttcaagcat gcatggtgat catgtgacat ggcaagccaa
     8701 agcgcctata c
//
LOCUS       DATMZD010000094         6522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_83354,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000094 DATMZD010000000
VERSION     DATMZD010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6522
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83354"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="VN478_01955"
     CDS             complement(<1..564)
                     /locus_tag="VN478_01955"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HWQ21474.1"
                     /translation="MTKRKSFRRVSVLGLALLLIVFSLVGCARRNSVSLRIVSAYSGK
                     GVGNATLTFTGSSGSESLSSDATGMVSGRVEADRIQMTVEAAGYQNGFVQVADLTQLP
                     STVQLTPLFLGAGTVTSAGQPIAGASVTVWNTTTTTRADGTFTFEGLAEGTYAGRVTK
                     QGFADATFNLAVGKSAKPVKVALEEGQL"
     gene            complement(568..1098)
                     /locus_tag="VN478_01960"
     CDS             complement(568..1098)
                     /locus_tag="VN478_01960"
                     /inference="COORDINATES: protein motif:HMM:NF039198.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5698 domain-containing protein"
                     /protein_id="HWQ21475.1"
                     /translation="MAQFLVVAESVLIVFFMKFIDMALSTLFTNFLVRGRRLFTFIFG
                     FLEVMVYLVSLSRVLNNMSIYKMLGYALGYSFGALLGIWLEQKLAIGNQTWLIIPNEN
                     PYRLAYHLRDRGFGITTISGSGMDSNRMIILTTTMRKNLALLNASLTELAPDAFVSVL
                     DTRTTMGGHVTRIPRG"
     gene            1279..2175
                     /locus_tag="VN478_01965"
     CDS             1279..2175
                     /locus_tag="VN478_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21476.1"
                     /translation="MTRPLRIEFPGAVYLVSDIVRAGRSVLQSDDARTQFLAELAIVS
                     ASLDWHIYAWCMLPDRYSLVVETPRGDLGKGMRRFISSYTRHVNPQSGSSGRIFRGAY
                     RVILLEPDVWLLPLCRHVVLQPVASGVVATPEEWPWSSYQATVMSTHVHSAPLDADSL
                     LSRFGQTQEDAVAAYADYMRVGVGLPSPLTHVTPPGVLGTQTFVQVVTEHLVGRASDP
                     SVAATLLMLARPSLAQIFGPDVRRERSQLPLRVAAAVHLGYSLVQIGDHLGVHYTTVS
                     RYLRAAQGGQDRTPPPVPSQAT"
     gene            complement(2284..2463)
                     /locus_tag="VN478_01970"
     CDS             complement(2284..2463)
                     /locus_tag="VN478_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HWQ21477.1"
                     /translation="MYRSRKNQVIGGVAAGVAEYFDIDPTIVRIAWALLGMAWGTGVL
                     VYLICWLVIPLNPAQ"
     gene            complement(2694..3224)
                     /locus_tag="VN478_01975"
     CDS             complement(2694..3224)
                     /locus_tag="VN478_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21478.1"
                     /translation="MNNSNIDRRVALSTLLIVFGIHFLLQAFPQTGYHFGILVPLAAF
                     VILLGIYGVATGFQHDSRETVFWSGMLGFVGIIVLLQVLGVIRFSLTTFAGAAVVSAG
                     LSILMSTLFDSVEGRTPRNGLIWGVAAVAIGSLAFLSGLDIFSVQVVDIIRKSAVGGL
                     FLVLGLAVLIKGGTKK"
     gene            complement(3221..4093)
                     /locus_tag="VN478_01980"
     CDS             complement(3221..4093)
                     /locus_tag="VN478_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21479.1"
                     /translation="MMQPAFMTGRRHVLVALLVVGMILAGSVVVTLVTRNGGFRPAPA
                     LAEGAILEVPGGSASSISVVGDNLEITVSTSSAVTSPSIVVGSEGFRQDFRAKAGTFG
                     LRLGGLSASQTGVRRLTVVVPENGTLTLTLVGEARLVMDQARLKELRLAGASSALELD
                     ASESGSIVDLVQIENVTGGFSTTHLGNLDMADLVIGDITGTYDLDFSGSLNRHCDVSL
                     HTAVGNGIVRIPSKPGAQLSVGSVLGNVLSSGFVAGTGKSFVNTAAAGGADRQLVVRI
                     DSAIGQIQLVEVQQ"
     gene            complement(4090..4545)
                     /locus_tag="VN478_01985"
     CDS             complement(4090..4545)
                     /locus_tag="VN478_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21480.1"
                     /translation="MKTQHLTDEELELILSSTLSCPPELVPPPGLACGVWERIDRWEE
                     EQERRRVSRSILARLMGTRMRNGEPVVVLAGALLFAVLFVGMFLTGTYMIAAHSSVVM
                     RVIQLVVGPNLEDLRSLAVLCTLTSVGGLLLGSLALSERLFGQSGGSPA"
     gene            complement(4542..5054)
                     /locus_tag="VN478_01990"
     CDS             complement(4542..5054)
                     /locus_tag="VN478_01990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HWQ21481.1"
                     /translation="MEAVADRELFAQLMAESERPVYAFLASKVRDRTIASDISQEAFF
                     LAYRRFDSYDRSRPFLPWVMTIAYNCLVDYWRKLAHEGGDLPDEQLLAAPEQDRDDEW
                     KQQEIVEAMTRVSPDESALLTMFYAEGSSLATIGVQLGVAEGTAKVRLFRARAALKKQ
                     VEALHERGAS"
     gene            complement(5137..>6522)
                     /locus_tag="VN478_01995"
     CDS             complement(5137..>6522)
                     /locus_tag="VN478_01995"
                     /inference="COORDINATES: protein motif:HMM:NF015736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HWQ21482.1"
                     /translation="SFQWYFSNHKGDGGKIDLPLFTAYLTEERHVTPGVAALVGKLAD
                     AGGEDMTTLIELLDRLKTRAARRKLRTIADSISSYTDAEKQGRRVEDFAAEVSEQLRQ
                     VARLSSQNTQEPIRDDLLRYVEEVRTRETGRPSIIGLSIAPYDNLNTAISGLRPGFYY
                     ALGGAPRRGKTNLMLRLATLVARNEKVPVLYYSFEQTKRVLLTRVLSQESLINPLVLQ
                     KGDIRNKAELVTRFNKGIKETSAYMDNFYLFEGDRQDTVEHIRSVAYSIMDSHHAERC
                     AIFLDYLQKVPTVNPYASLQKQVDEVSGLIAQLSTELQSPIVAVSSFDKDGCRLDSEV
                     SKDRPTMFNCTGGGDIEYDADVALIIVKDYHDTAQLEEKIANAVREGGVNPHHIPHFD
                     ILNLFIDKNRDSPEGGNIIVQFLFLIEDNQMVELGYKDVDERYSYAKAGKIFEWLLER
                     GYLEAVGPGGH"
BASE COUNT         1316 a         2063 c         1884 g         1259 t
ORIGIN      
        1 gagctgcccc tcctcgagcg ctactttcac gggcttcgcg ctcttgccga ccgccaggtt
       61 gaaggtcgca tcggcgaacc cctgcttcgt gaccctgccc gcgtaggtcc cttcggcgag
      121 tccttcaaag gtgaacgtcc cgtccgccct ggtcgtcgtc gtcgtgttcc acacggtcac
      181 cgacgctcct gctatcggct ggcctgctga cgttacggtc cctgcgccaa gaaacagcgg
      241 agtcagctgg acagtcgacg gcagctgcgt gaggtccgcc acctggacga acccattctg
      301 gtagcctgca gcttcgacgg tcatctgtat ccgatccgcc tccactcgtc ccgagaccat
      361 tcccgtcgca tcagagctga gcgattcgga accgctggac ccggtgaacg tcagcgtcgc
      421 attcccaacg cccttgcccg aataggcaga gacgatcctg agcgagacac tgttgcggcg
      481 ggcacagcca acaagcgaga agacaataag cagcagagca agaccgagca ccgacactcg
      541 gcggaacgac ttccttttcg tcatgattca acctctaggg attcgagtca catgcccacc
      601 catcgtcgtc cgcgtatcca gcacggacac gaaagcgtcc ggtgccagct cggtgagtga
      661 ggcgttaaga agtgccaggt tcttgcgcat tgtcgtcgtc aggatgatca tgcggttgct
      721 gtccatgccg gatccggaga tcgtcgtgat gccaaagccc cggtcacgca ggtgatacgc
      781 cagtcggtac gggttctcgt tggggatgat cagccatgtc tggttgccga tggcaagctt
      841 ctgttcgagc cagatgccaa ggagtgcgcc gaatgaatag cccagggcat accccagcat
      901 cttgtagata ctcatgttgt tcaggaccct ggaaaggctg accagataga ccatcacttc
      961 gagaaacccg aagatgaacg tgaacaggcg ccgtcccctg acaaggaagt tcgtgaagag
     1021 agtcgagagg gccatgtcta tgaacttcat gaagaacacg atcaggactg actcggctac
     1081 caccaagaac tgcgccacgc gaaacctccc gttcccgtac tgacaggttc aagtataccg
     1141 gccgctgacc gctgcacaag ctggacgaga agaagacctg tgccggggga tcgcgcattg
     1201 aaatgttgcg ctgctgtgga taacatgtga gcagtgtgga cagacccgtc atccacgcct
     1261 ctcgcgaaag gagggtttgt gactcgaccg ttgcgcatcg agtttccagg tgccgtgtat
     1321 ctcgtttccg acattgtccg ggccggtcgc tccgttttgc agagcgacga cgctcgcacg
     1381 caattcctcg cagagcttgc gatcgtgtcg gccagcctcg attggcacat ctacgcctgg
     1441 tgcatgctgc cggatcgcta cagcctggtc gtcgagactc cccggggaga cctcggcaaa
     1501 ggcatgcgcc gcttcatcag cagctacacg cggcatgtga accctcagag cggatcctcg
     1561 gggcggatct tccgcggggc ctatcgcgtc atcctcctgg aaccagatgt gtggctcctg
     1621 ccgctctgtc gccacgtcgt gcttcagcct gtggcttcag gggtcgtggc gactcctgag
     1681 gaatggccct ggagcagcta ccaggcaaca gtcatgagca ctcacgtgca cagtgcccct
     1741 cttgacgccg attcactact gtctcgcttc ggccagactc aggaggatgc tgtcgccgcc
     1801 tatgccgact acatgcgggt gggtgtcggg ctgccgtccc ctctcacaca cgtgacgccg
     1861 ccgggagtcc tgggcacaca gacgttcgtc caggtggtca ctgaacacct ggtgggtaga
     1921 gcgtcagatc cgtcagtcgc ggcaacgctc ctcatgcttg cgcgtccttc cctggcccag
     1981 atctttggac ccgacgtcag gcgcgagcgc tcacagctcc cgttgcgcgt agctgcagcc
     2041 gtccatctcg gctactcgct ggtacagatc ggcgaccacc tgggcgtcca ctacacgacg
     2101 gtcagccgct accttcgcgc agctcaggga gggcaggaca ggacacctcc cccggttccc
     2161 tcccaggcca cgtgaaagca ctctcccggc agcttgcaga ggccgccggg agagtgtcgc
     2221 ccgtcaagac gaccggactt ttctacacat tatccacaga gatgttatgt ttctaccacc
     2281 gacttactga gcagggttca acgggatcac gagccagcag atgagataga ccaggactcc
     2341 cgtgccccat gccattccga gcaatgccca ggcaatgcgc acgatcgtcg ggtctatatc
     2401 gaagtactcc gctacgcccg ccgccacgcc gccgatcacc tgattcttgc gtgagcggta
     2461 caacctcttg gaggaatcgt atccggttcc tgaagggctc gaacgctcag cctgcggatg
     2521 ctgccactgg tcctggatcg ggcccgcctc gccgaaaccg gaagccggct gcgccggcgc
     2581 gcaggtaccg cacatgacct tgccgtccac catgactact tcctcgcggg caagcgcctt
     2641 tccacacttg gtgcagacgc caaccgcttc ttcatctggt ctgtcaaaac tcttcatttc
     2701 ttcgtacctc ccttgataag cacggccaat ccgagtacga ggaacagtcc cccgactgcc
     2761 gatttccgaa tgatgtcgac cacctggacc gagaagatgt ccagacccga aaggaacgcg
     2821 agagagccaa tggcgacagc cgcgactccc cagatcaacc catttctcgg cgtcctgcct
     2881 tcaaccgagt caaacaacgt cgacataagg atggagagcc ccgccgagac cacggcggct
     2941 cctgcaaacg tcgtcaggct gaaccggatg accccgagga cctgcaggag aacgatgatg
     3001 ccgacaaatc caagcatgcc gctccagaat acggtctcgc gggaatcgtg ctggaagccg
     3061 gtcgcaacgc catagatgcc cagcaggatc acgaacgccg ccagtgggac aaggatgccg
     3121 aaatggtatc ccgtctgggg gaacgcctgg agcaggaaat ggataccgaa cacgatgagc
     3181 agagtgctca gggctacccg ccgatcaatg ttgctgttgt tcattgctgc acctccacaa
     3241 gttgaatctg cccaattgca ctgtcgatac gcaccacaag ctgccggtca gccccgccgg
     3301 cggccgcagt gttgacgaaa gacttgcccg tcccggctac gaatcccgac gacaggacgt
     3361 tgcccaggac gcttccaaca gaaagctgtg ctcccggctt gctgggaatg cgcactatgc
     3421 catttcccac ggccgtgtgc agtgagacgt cgcaatgtct gttcagcgac cccgagaagt
     3481 cgagatcgta ggtaccggtg atgtctccga tgacaaggtc ggccatatcg agattgccaa
     3541 ggtgtgttgt cgagaacccg ccggtgacgt tctcgatctg aacgaggtcc acgatggacc
     3601 cggactcgga ggcatccagt tccagcgcgg aggatgcgcc cgccaggcgg agctccttga
     3661 gccgggcctg atccatgacc aggcgggcct caccgacaag cgtcagcgtc agcgtgccat
     3721 tctcaggtac cacgacagtc aggcgcctga cccctgtctg agaagcggag agaccgccga
     3781 gacgcaatcc aaacgtgcca gccttcgcgc ggaaatcctg gcggaacccc tcactgccca
     3841 cgacgatgct gggtgaggtc acggccgacg acgtactcac ggtgatctca aggttgtcgc
     3901 cgacgacaga tatgctgctc gccgagcccc ccgggacttc caggatcgcg ccctcggcaa
     3961 gcgccggagc aggcctgaat ccgccattcc gcgtcacaag cgtcacgacc acggacccgg
     4021 cgagaatcat gccgacgacg agcagagcga ccagaacgtg tctgcgccct gtcatgaaag
     4081 cgggctgcat catgcaggcg accctccact ttgcccgaac agacgttcac tgagggccag
     4141 actgccaagc aggagcccac ccaccgacgt cagcgtgcac aggactgcga gagaccggag
     4201 gtcttcaagg ttgggaccga cgacaagctg tatgacacgc atcacgactg acgaatgagc
     4261 tgcgatcatg taggtcccgg tcaggaacat accgacaaac aggaccgcaa acagcagcgc
     4321 gccggcaagg acgacgaccg gttctccgtt cctcatgcgt gttcccatga ggcgggccag
     4381 gatgctgcgt gacactctcc tccgttcctg ctcctcttcc cacctgtcga tacgttccca
     4441 gacgccacat gcaagtcctg gcggagggac aagttcggga gggcatgaga gggtactgga
     4501 aaggataagt tccagctctt cgtctgtcag atgttgtgtc ttcatgaagc acctcgctcg
     4561 tgcagggctt ccacctgctt cttcaatgcg gctcgtgccc gaaacaaccg gactttcgcc
     4621 gttccttccg caacgccgag ctgtacaccg atcgtcgcaa gagaagaccc ttcggcatag
     4681 aacatcgtca gcagtgcgct ttcgtcagga gacacgcgtg tcattgcctc tacgatctcc
     4741 tgctgtttcc actcgtcgtc acggtcctgc tcgggagccg ccaggagttg ctcgtccggc
     4801 aggtccccgc cctcatgcgc caacttccgc cagtagtcga ccaggcagtt gtaggcaatc
     4861 gtcatgaccc acgggagaaa ggggcggctt ctgtcatacg agtcgaacct cctgtatgcc
     4921 aggaagaacg cttcctgcga aatgtccgac gctattgtcc gatcccggac cttgctcgcc
     4981 agaaacgcat acacaggacg ctcactctcc gccatcaact gggcgaagag ctcgcggtca
     5041 gccactgctt ccatgcacct gctcctctcc gcggtcgatt gctctacgtg ttcaccaggc
     5101 gtacggtgac gggagagaaa aggttacaga aagggctcag tgcccgcccg ggccgaccgc
     5161 ctcgagatac ccgcgttcga gcagccactc gaagatcttg ccggccttgg catacgaata
     5221 gcgttcgtcg acatccttgt agccaagctc caccatctgg ttatcttcga tcaggaagag
     5281 gaactggaca atgatgttgc ctccctcggg agaatcgcgg ttcttgtcga tgaacagatt
     5341 gaggatgtca aagtggggga tgtggtgggg gttgacccct ccctctcgca ccgcgttggc
     5401 gatcttctct tccagctgag ccgtgtcgtg gtagtccttc acgatgatca gggcaacgtc
     5461 cgcatcatat tcgatgtcac caccacctgt gcagttgaac atcgtagggc ggtccttgga
     5521 cacctcgctg tcgagccggc acccgtcctt gtcgaacgac gaaaccgcca cgatgggaga
     5581 ctgcaactcc gtggagagct gcgcaatgag gccggatacc tcgtcgacct gtttctgaag
     5641 gctggcatag gggttgacgg tcggcacctt ctgcaggtag tcgaggaaga tggcgcagcg
     5701 ctctgcatgg tgtgagtcca tgatgctgta tgcaacactg cgaatgtgct cgaccgtgtc
     5761 ctggcggtcg ccctcgaaga ggtagaaatt gtccatgtag gccgacgtct ccttgatccc
     5821 cttgttgaac cgcgtgacaa gctcggcctt gttcctgatg tcgcccttct gaaggaccag
     5881 cgggttgatg agcgattcct gcgacaagac acgcgtcagg agcacacgct ttgtctgctc
     5941 gaaggagtag tacagcacag ggaccttctc gttccgtgcg acgagcgtgg caaggcgcag
     6001 catcaggttg gtctttccac gcctgggggc ccctccgagt gcatagtaga agccaggccg
     6061 caatcccgag atcgcggtat tgaggttgtc gtagggagcg atactcagcc cgatgatcga
     6121 cggacgcccc gtctcgcggg tgcgaacctc ttcgacgtat cgcagcaggt cgtcacggat
     6181 cggctcctga gtattctggg aagagaggcg tgccacctgg cgcaactgct ccgagacctc
     6241 tgcagcaaag tcttccacgc gccgcccctg cttctcggcg tcggtgtagc tgctgatgct
     6301 gtcggcgatc gtgcgcagct ttcgacgtgc cgcgcgcgtc ttgagccggt ccagcagctc
     6361 gatcagcgtc gtcatgtcct cgccgccggc gtcggccagc ttgcctacca gcgccgcaac
     6421 gcccggagtc acgtgacgct cctcggtcag gtatgccgtg aacaggggaa ggtcgatctt
     6481 tccgccgtcg cccttgtgat tggagaaata ccactgaaag ga
//
LOCUS       DATMZD010000095         6011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_22818,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000095 DATMZD010000000
VERSION     DATMZD010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6011
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22818"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VN478_02000"
     CDS             <1..267
                     /locus_tag="VN478_02000"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HWQ21483.1"
                     /translation="IRRMEKAGLVCKSTDPDDQRRNHLAITDKGLLLVEDCLRAFHYV
                     DQGMFKGFSEEERRQLESSYRRMMENLVAMGAQVPTFMNQEEKK"
     gene            264..2030
                     /locus_tag="VN478_02005"
     CDS             264..2030
                     /locus_tag="VN478_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWQ21484.1"
                     /translation="MIRKLWAHLGTYKRLLLLSPLFVMVDVVCELTMPMLMGRIVDKG
                     IPTLNLQYIVGVGVLMIGLALVAMLFGSLNHRLAAVTSQGYGANLRQALFDKVQSFSF
                     SNIDTFSTASLVTRLTNDVTQIQNTLLMAMRILTRAPLMLICALVFAIAINAKLAVIL
                     FIAIPTLVAGISLVIGQAERLFQAVQAKIDAVNSTVEENLIGIRVVKAFVRERHEKVK
                     FKKSNDELSEKAVKAGNLVVSIMPIMMLVLNGATVAVIWFGGRMVGTHQMGTGELVSF
                     IAYIMQILMSVLMVSMILMMLARARASAERIIEVLDTIPSIADSEPALATGAAAAAEE
                     PMVTYGAVEFRHVDFKYATSGKGENVLSDIDLALKPGEFVALVGGTGSGKSTLVSLIP
                     RLYDVTDGQVLVDGVDVRDYKLATLRSAIGVVLQNNVLFSGTIRENLMWGNAAATQQE
                     IEQAARNAQAHDFIMSFPDGYDTDLGQGGVNVSGGQKQRLCIARALLKKPRILILDDS
                     TSAVDSATEARIRASFRENLADTTILIIAQRISSVQQADKIIVLDDGRIAGIGTHAEL
                     LAGNPIYQAICASQMEGVVVNG"
     gene            2023..3936
                     /locus_tag="VN478_02010"
     CDS             2023..3936
                     /locus_tag="VN478_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016322631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWQ21485.1"
                     /translation="MAEQTRPIMPTRGAGPRMAFQKPKNVGATTRRILTYFGSTRYAL
                     IVVCILLIVSTGSGLAGSYFLKPLVNKYILPGNFTGLAGALVVLAAIYLVGVVASYMQ
                     GRVMVSIGQKAANVMRRDLFDKLQDLPLKVFDTHTHGELMSRFTNDIDNVEMAIEQSV
                     VQLISGVLTFVGTLVMMLVLSPILFAVAAVVLAFMIFLSTTLSKKSTGYFRTQQKSLG
                     TLNGYIEEMVDGLKVVKVFGHEGVASDEFSGLNEQYRSSATTATFLSGVVMPLMGNLS
                     RICYATVAIVGGLMAVGGRLDTGSLVAFLQLSYQVGQPINQITNQLSVVLAALAGAER
                     IFDIMDQQPEVNDGTVTLVNVTYAEDGTEDGTIVETPTGTKTSEWAWSVPTDHGHRLV
                     PLKGDVRFDNVTFSYDGVTPVLKDISLYAKPGQTIAFVGSTGAGKTTITNLINRFYDI
                     QHGTITYDGIDVKTIRKDDLRRSLGMVLQDTHLFTGTVMDNIRYGRLDATDDECLLAA
                     KRASADSFIRRLPQGYDTVITGDGSNLSQGQRQLLAIARAYVADPPVMILDEATSSID
                     TRTERLIEHGMDALMEGRTVFVIAHRLSTVRDAKAIIVLEHGEIIERGNHESLLAERG
                     LYYQLYTGQSALE"
     gene            complement(3980..4624)
                     /locus_tag="VN478_02015"
     CDS             complement(3980..4624)
                     /locus_tag="VN478_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21486.1"
                     /translation="MRRIVLVVMILAGIVIMLAGCNTTRKANMEESLRWDNPVGYVTS
                     NDVKQVTKGMTYADIITQLGPTRDIGSGLHVAHYIVDDTKSLYLSFGNPEDICPKDGV
                     GLLQSLEAIDFSTIGIRGVVKDIVHGKDGITMLVEGKQETDTALTAANVTVTMQSRVY
                     RGQTLIEGSFGFSEIKEGDTVEVTFAGPVTMSYPVMGVAAIVRILMQSLNSEPA"
     gene            4873..5295
                     /locus_tag="VN478_02020"
     CDS             4873..5295
                     /locus_tag="VN478_02020"
                     /inference="COORDINATES: protein motif:HMM:NF024055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HWQ21487.1"
                     /translation="MNARVIYQSTTGNTKRVADAMAAAAGCKAESVTDAVISEPVDML
                     FLGASIHGGDIDALVKKFIEGLDPALVKQVTVFGTGFPENKEKAVGIMKGLLAQRSIA
                     VTDKCYFCRGKFLLFNRKSPDDQDLKGAEEFARSVLTD"
     gene            complement(5313..>6011)
                     /locus_tag="VN478_02025"
     CDS             complement(5313..>6011)
                     /locus_tag="VN478_02025"
                     /inference="COORDINATES: protein motif:HMM:NF015548.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HWQ21488.1"
                     /translation="ALDNLHSVYVDQWDWERVIDADERTVPFLEEIVRDIYGVLLRTN
                     RLVHERYPSVPPLDLPDEVAFVSTEDLEADDPDHAPQERVDRLCHELGAVFVIGIGAP
                     LRNGVPHDGRAADYDDWSTPTDETHRGLNGDLYVWNPVLGCAFELSSMGIRVDPCALL
                     RQLEARGELASRNLPYHRRLLAGDLPLTIGGGIGQSRLCMLLLHTAHIGEVQSGVWSS
                     AMRSACSAAGIPLL"
BASE COUNT         1232 a         1870 c         1775 g         1134 t
ORIGIN      
        1 atcaggcgca tggagaaggc gggtctcgtc tgcaagtcga cggatcccga tgaccagcgt
       61 cgcaaccacc tcgccattac cgacaagggg ctgcttcttg tcgaggattg tcttcgagca
      121 ttccactacg tcgaccaggg catgttcaag ggattctcgg aagaggaacg ccggcagctg
      181 gagagcagct atcgccgcat gatggaaaac cttgtggcta tgggtgcaca agtgccgaca
      241 ttcatgaacc aggaggaaaa gaagtgatca ggaagctgtg ggcccatctg ggcacgtaca
      301 agcggctgtt gctcctttcg ccgctctttg tcatggtcga tgtcgtctgc gaactgacca
      361 tgccgatgct gatgggcagg atcgtcgaca agggcatacc gacgctgaac ctgcagtaca
      421 tcgtcggcgt cggcgtgctg atgataggcc tggcactggt cgccatgctg ttcggctcac
      481 tcaaccatcg gcttgcggcc gtcaccagcc aggggtatgg cgccaatctg cgccaggcgt
      541 tgtttgacaa ggtccagtca ttctcgttct cgaacatcga cacattcagc acggcgtctc
      601 tggtcacccg tctcacgaac gacgtgaccc agatccagaa caccctgctc atggcaatgc
      661 gcatcctgac ccgtgccccg ctgatgctca tctgtgcgct cgtcttcgcg atcgccatca
      721 atgccaaact tgccgtcatc ctgttcattg ccattcccac actcgtcgca gggatatcgc
      781 tggtcatcgg ccaggccgaa cgcctcttcc aggcagtcca ggcgaagatc gatgccgtga
      841 acagcacggt cgaggagaac ctgatcggca ttcgtgtcgt caaggccttt gtccgcgagc
      901 gtcacgaaaa ggtcaagttc aagaagtcca acgacgaact gagcgagaag gcggtcaagg
      961 caggcaacct cgtcgtctcc atcatgccca tcatgatgct tgtcctcaac ggcgcgaccg
     1021 tggccgtcat ctggtttgga ggccgcatgg tcggcacgca ccagatgggc acgggagaac
     1081 tcgtgagctt catcgcctac atcatgcaga tcctcatgag cgtgctcatg gtttccatga
     1141 tcctcatgat gctggcccgc gcgcgcgcaa gcgccgagcg catcatcgaa gtgctcgaca
     1201 cgatccccag tatcgccgac agtgagccgg cgctggcaac cggcgcggct gccgcagcag
     1261 aggagccgat ggtcacgtac ggcgccgtcg agttccgtca tgtcgatttt aagtacgcaa
     1321 cgagcggcaa gggcgagaac gtcctgtcgg acatcgatct tgctctcaaa cccggcgaat
     1381 tcgtcgccct ggtaggtggc acaggttcgg gaaagtcgac gctggtgagt ctgatcccac
     1441 gcctgtacga tgtgaccgac gggcaggtcc tcgtcgacgg cgtggacgtg cgggactaca
     1501 aactggcgac gctgcggagc gcgatcggcg tcgtcctgca gaacaacgtt ctgttctccg
     1561 gcacgatccg cgagaacctg atgtggggca atgcagcggc gacgcagcag gagattgagc
     1621 aagcagcccg caatgcccag gcccatgatt tcatcatgag cttcccggac ggctacgaca
     1681 ccgaccttgg acagggtggc gtcaacgtct ccgggggtca gaaacagcgc ctatgcatcg
     1741 cgcgcgccct gctcaagaag ccgcgcatcc tgatcctcga cgacagcacg agcgcggtgg
     1801 attcggccac cgaggcaagg atccgcgcat cgttccgtga gaaccttgcg gacacgacga
     1861 tcctgatcat cgcccagcgc atcagctcgg tgcagcaagc cgacaagatc atcgtcctgg
     1921 atgacggccg cattgccggg atcggaacac acgccgaact gctggcgggc aacccgatct
     1981 accaggcgat ctgcgcatcg cagatggaag gagtggtcgt caatggctga acaaacgcgt
     2041 ccgatcatgc ccacccgtgg cgccggtccc cgcatggcgt tccagaaacc caagaatgtc
     2101 ggtgcgacca cacgccgcat tctgacctat ttcggcagca ccaggtacgc actcattgtc
     2161 gtctgcattc tgctcattgt cagcaccggc agcggacttg ccggatcgta cttcctgaaa
     2221 ccactggtca acaagtacat tctgccgggc aacttcacag gacttgccgg cgctctcgtc
     2281 gtcctggccg ccatctacct ggtcggcgtc gtggcctcct acatgcaggg ccgcgtcatg
     2341 gtgtccatcg ggcagaaggc cgccaatgtc atgcgcaggg acctgttcga caaactgcag
     2401 gatctgccgc tcaaggtctt cgatacccat acccacggcg aactcatgag ccgcttcacc
     2461 aacgatatcg acaacgtcga gatggcgatc gaacaaagcg tcgtgcagtt gatctcgggc
     2521 gtcctgacct tcgtcgggac cctggtcatg atgctggtgc tcagtccgat cctgttcgcc
     2581 gtcgcggcgg tcgtcctggc gttcatgatc ttcctgagca ccacgctcag caagaagagt
     2641 acaggctact tccgtaccca gcagaaatca ctcggtaccc tcaacggcta cattgaagag
     2701 atggtcgacg gtctgaaggt cgtcaaggtg ttcggacacg aaggcgtggc cagcgacgag
     2761 ttctcgggcc tcaacgaaca gtaccgctca tccgcgacca ccgcgacctt cctgtctggc
     2821 gtcgtcatgc ccctcatggg caacctcagc cgcatctgtt acgcaacagt tgccatcgtc
     2881 ggtggtctca tggccgtcgg cggccgcctc gataccggtt cgctggtcgc cttcctccag
     2941 ctgtcgtacc aggtcggcca gcccatcaac cagatcacga accagctcag cgtcgttctt
     3001 gccgctctcg cgggcgccga gcgtatcttc gacatcatgg accagcagcc cgaggtcaac
     3061 gatggaacgg tcacgctagt caacgtcacc tatgcggaag acggcacgga ggacgggacg
     3121 atcgtcgaga cgccgacagg cacgaagacc agcgaatggg catggagcgt cccgaccgat
     3181 cacgggcacc ggctggtgcc cctcaagggc gacgtccggt ttgacaacgt cacgttctcg
     3241 tacgacgggg tgacccctgt cctcaaggac atctcgctct atgccaaacc gggacagacg
     3301 atcgcctttg tcggttcgac cggcgccggc aagacaacga tcacgaacct gatcaaccgt
     3361 ttctacgaca ttcagcacgg caccatcacg tacgacggca tcgacgtcaa gacgatccgc
     3421 aaggacgacc tgcgtcgctc cctgggcatg gtgctgcagg atacgcacct gtttacgggc
     3481 actgtcatgg acaacatccg gtatggtcgg ctcgacgcta cggacgacga gtgcctgctg
     3541 gccgcgaagc gtgccagcgc ggactcattc atccgccgtc tgccgcaggg gtacgatacg
     3601 gtcatcaccg gtgacggaag caacctgtcc cagggtcagc gccaactgct cgcgatcgcg
     3661 cgcgcctacg tcgccgatcc gcccgtcatg atcctcgacg aggccacgag ttccatcgat
     3721 acgcgaacgg agcgcctgat cgaacacggc atggacgcac tcatggaagg caggacggtg
     3781 ttcgtcatcg cccatcgcct gtccacggtc cgcgatgcca aagcgatcat cgtcctcgag
     3841 catggcgaga tcatcgaacg cggcaatcac gagagtctgc tggccgagag aggcctgtac
     3901 taccagctgt acacggggca gtccgccctc gagtaacaac tgtcactgaa cgtcagaacc
     3961 cgcgtgacct gatgcaggcc tacgcgggtt cgctattcaa tgactgcatg aggatacgca
     4021 cgatcgccgc gacacccatc accgggtagc tcatggtcac ggggcccgca aatgtgacct
     4081 cgaccgtgtc tccttccttg atctccgaga agccgaacga cccctcgatc agtgtctgtc
     4141 cccggtacac gcgggattgc atcgtgacag tgacattggc cgcggtgagt gcggtatctg
     4201 tctcctgttt cccctcgacc agcatggtga tgccgtcctt gccatgcaca atatccttga
     4261 cgactcccct gatgccaatc gtcgagaagt cgatagcttc gaggctctgc agcagcccaa
     4321 ccccatcttt cggacaaata tcctctggat tgccgaatga gagatacagg ctcttcgtgt
     4381 cgtcgacgat gtagtgtgca acgtgcaggc cgctgccgat gtcgcgggtc ggaccgagtt
     4441 gtgtgatgat gtccgcgtag gtcatgccct tcgtgacctg cttgacgtca ttgctggtga
     4501 cataccccac ggggttgtcc caccgcagcg actcttccat attcgccttt ctggtcgtat
     4561 tgcacccggc cagcatgatc acaatgcctg ccaatatcat cacaacaagc acaatccgcc
     4621 tcatggtcca cccccactcg tcataacgtc tcagtgctct tccctgctac gagtatgaca
     4681 cagaaacctg gacggagttc ccacggtggc atcacggccg gccacagtag tctgtcaccg
     4741 ctacccggtt tgttcgttct gagatatcct gtgggaagac atcggggacc atcggccgat
     4801 ccctgactgt ccaggaggaa aggcccgccg ggctcccctg agccaggaca tgcagagacg
     4861 gatggaggta tcatgaacgc acgagttatc taccaatcga cgacaggcaa tacgaagaga
     4921 gtagcggatg ccatggcggc cgcggctggc tgcaaggcag agtctgtaac tgacgccgtc
     4981 atcagcgagc ccgtggacat gctattcctg ggggcatcga tccatggcgg agatatcgat
     5041 gcactggtga agaagttcat cgaggggctc gatccagccc tcgtcaagca ggtgacagta
     5101 ttcggcaccg gatttccgga gaacaaggag aaagcagtcg ggatcatgaa gggcctgctg
     5161 gcccagcgga gcatcgccgt gaccgacaag tgctactttt gcaggggcaa gttcctgctc
     5221 ttcaaccgca agagcccgga cgaccaggac ctcaaagggg cagaggagtt cgcgcggagc
     5281 gtgcttacag actagtacct cgcgcgtcgt ctctagagca gaggtatccc tgcggcggaa
     5341 caggcggacc gcatggcgct ggaccagaca ccggactgga cctctccgat gtgtgccgtg
     5401 tgcaggagaa gcatacagag cctcgactgt ccgatgccgc cgccgatggt caatggcagg
     5461 tcgcccgcga gcaggcggcg atgatatgga aggttccgac tggcgagttc gccgcgtgct
     5521 tcaagctgac gcaggagggc gcacggatct acccggatgc ccatcgacga gagctcgaat
     5581 gcacaaccga ggaccgggtt ccagacatag aggtctccgt tgaggccgcg atgcgtctcg
     5641 tccgtgggcg tcgaccagtc atcgtagtcg gctgcacgtc catcgtgggg aacgccgttg
     5701 cgcagagggg cgccgatacc gatgacgaac acggcgccca actcatggca gaggcggtcc
     5761 acccgctcct gcggcgcgtg atcggggtcg tcggcctcca gatcttcagt ggacacgaag
     5821 gcgacctcgt ccggcaggtc cagaggtggt actgaagggt agcgctcatg cacgagccgg
     5881 ttggtgcgga gcaggacacc gtagatgtct cgcacgatct cctcgaggaa cggcacggtc
     5941 cgctcgtccg cgtcgatgac gcgttcccag tcccactggt cgacgtagac ggagtgcagg
     6001 ttgtccagtg c
//
LOCUS       DATMZD010000096         4401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_12783,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000096 DATMZD010000000
VERSION     DATMZD010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4401
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12783"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="VN478_02030"
     CDS             <1..544
                     /locus_tag="VN478_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HWQ21489.1"
                     /translation="ATLAQTVVDSGGAVVSEFHLHMQPTVYTFPQRNRVISGLSLGVV
                     VVEAARQSGSLLTANFALEQNREVFAVPGSIYAPQHVGTNGLIKSGAKLVQCVADILD
                     ELPQFRAATLDDEVPCAALSAPEQAVVDCIQSGGTSVDLLCQRLGYATPQVLTILTGL
                     ELRGFLVRLGPDSLAIARRP"
     gene            546..1862
                     /gene="trmFO"
                     /locus_tag="VN478_02035"
     CDS             546..1862
                     /gene="trmFO"
                     /locus_tag="VN478_02035"
                     /inference="COORDINATES: protein motif:HMM:NF003739.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HWQ21490.1"
                     /translation="MGPDIRVVGGGLAGSEAALTLARLGLAVELWEMRPSHQTDVHET
                     DQFGELVCSNSLGSDALRDARGLLKAELRLLGSAVVACADASRLPAGKAFAVDKNQFS
                     GLVTQSIQTNPHILIRREEYTRLDLSLPTIIATGPLTSVDLLSELRMRLGRENLFFYD
                     AIAPSVWATSVDSSCSFLSSRGGEAADYLNCPLTKDEYYAFVHALQSARQHLPETHDE
                     QKYFEGCLPVEEIARRGDDALRFGPMRPVGLRERPENCFAVVQLRKENRDGSVLGLVG
                     FQTSLAVSEQQRVLGMVPGLHHAKYVRFGLIHRNAFLRGPSVLSQDLQMAAFPRTWVA
                     GQLCGVDGYMESTALGLVAGLNAAALLRGFSRPALSTQTMTGLLVDYVTHEREDFQPT
                     NANYGLLPLEPGPKQKAVDREGFAAAALEHTRQVAAAVQAWTSESE"
     gene            1859..2863
                     /locus_tag="VN478_02040"
     CDS             1859..2863
                     /locus_tag="VN478_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWQ21491.1"
                     /translation="MTPDSGSVHGGGQDVATSFDVVAEMNGSYLQMPDAEFRARIRTA
                     YRRMSVCDLCPRHCGAHRLSGEVGRCGASLKPKVFVCNLHRGEEPPVSGTRGSGTVFF
                     SGCNLECCYCQNYPLSQLHHGTEMSVGRLAAALVELQRKGAHNINFVTPSHQIPQMLA
                     ATFAARQLGLHVPIVYNTSSYDELSALALLEGVVDVYLGDLRYTDESVGMQLSGVDDY
                     VRVAERALVEMHRQVGDILLDDQGRYVPRGLIVRYLILPRHTVMARQVFDFVLRRLSS
                     QTYVSLMTQYFPAYKALDRSLGISRKLTDTEYDRVMSAWLRSGLSHGWVQDMEEEGRA
                     "
     gene            complement(2922..3491)
                     /locus_tag="VN478_02045"
     CDS             complement(2922..3491)
                     /locus_tag="VN478_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015716872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylation factor-like protein"
                     /protein_id="HWQ21492.1"
                     /translation="MSLIDFSKSEITFKVVYYGPAMSGKTTNLSWIFRTIPEKVKGQF
                     TSIATETERTLFFDFLPVELGTVKGFKIRLSLYTVPGQELYKLTRKSVLKSVDGIVFV
                     ADSNRDRREDNINNLADMFKNLEEYELPDTPIIYQLNKRDMPGIMPVHELVSDLQLVN
                     KAVYLGIASGGVSVMECLKGITREVVSKI"
     gene            complement(3513..3992)
                     /locus_tag="VN478_02050"
     CDS             complement(3513..3992)
                     /locus_tag="VN478_02050"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HWQ21493.1"
                     /translation="MEGQLSSLVITKEDIETIDELFSQYLVDSQAKDIILLNKSGELL
                     AKSGAVTRESAVAVSALAAGVFSATNELAKLLGEREFTLTFHQGKETNIHISLVDPLI
                     LLAVIFDNKAPIGAIRFWAKKISGSIKPVLEKMASGPRQEPSQDLRKGLQDEIDNLF"
     gene            complement(3993..>4401)
                     /locus_tag="VN478_02055"
     CDS             complement(3993..>4401)
                     /locus_tag="VN478_02055"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HWQ21494.1"
                     /translation="VIHAQSPYSSGLPAFFDAFLEREGTFNFLSNVIMPPRRITESNT
                     SLLIEATRLADEYRQSEVQTEPSGLQVSLAENDSEAAVTLTADEFLLLQKAGERDSFD
                     RVLKEAEFGFFRAWAALNALADKKMVTVSKPEA"
BASE COUNT          870 a         1276 c         1290 g          965 t
ORIGIN      
        1 cgccacgctc gcgcagactg ttgtggacag cggaggagcg gtcgtttccg agtttcacct
       61 gcatatgcag ccaacagtct acacgttccc gcagcgaaac cgtgtcatct ccggtctttc
      121 gcttggtgtc gtcgtagtgg aagctgccag gcagagcggg tctctgctca cagccaactt
      181 cgctctggaa cagaatcgcg aggtctttgc ggttcccgga tcaatctatg cgccccagca
      241 tgtagggacg aatggactga tcaagtctgg tgccaaactc gtgcagtgtg ttgccgacat
      301 cctggacgag ctcccgcaat tcagggcggc gaccctcgac gacgaagtcc cgtgcgctgc
      361 tctgtctgcg ccggagcagg cagtcgtcga ctgcatacaa tcaggcggca cgtcagtgga
      421 cctgctgtgt caacgccttg gttacgcgac tcctcaggtt cttacgatcc tgaccggact
      481 tgaactccgt ggcttccttg tgcgactcgg accagacagt ctcgccattg cacggcggcc
      541 gtgacatggg cccggacatt cgagtggtcg gaggcgggct ggctggcagc gaggcagcac
      601 taactcttgc ccgacttggc cttgctgtgg aattgtggga gatgagacct tctcatcaga
      661 cggacgtcca cgaaaccgac cagttcggag aacttgtctg cagcaactcc cttgggtctg
      721 acgctctgcg ggatgcaagg ggtcttctca aggctgagct ccgactcctc ggttctgccg
      781 tcgtggcgtg tgccgacgcg agccgcctgc ctgcagggaa ggccttcgcc gtcgacaaga
      841 accagttcag tggtctggtg actcagagca tccagaccaa cccgcacatc ttgatccgca
      901 gggaggagta cacgaggctg gacttgtctc tgccgacgat catcgccaca ggccccctga
      961 cgagcgtgga cttgctgagt gagcttcgga tgcgccttgg gcgtgagaat ctgttcttct
     1021 atgatgccat cgctccgtcc gtctgggcga cttctgtcga ttccagttgc tcattcctga
     1081 gctcgcgagg gggagaggct gccgactacc tcaactgtcc tctcacgaag gacgagtatt
     1141 acgccttcgt ccatgccctt cagtcggcca ggcagcactt gcccgagact cacgatgaac
     1201 agaagtactt tgagggatgt ctgcctgtgg aggagatcgc acgacgcgga gacgacgctc
     1261 tcagattcgg cccgatgcgc ccggtgggcc tccgagagag accagagaat tgcttcgcag
     1321 tcgtccagct tcgcaaggag aaccgtgacg ggtctgtcct cggtctcgtt ggcttccaga
     1381 cgtctctcgc tgtctccgag cagcagaggg ttcttggcat ggtacccggt cttcatcacg
     1441 cgaagtacgt ccggttcggc ctcatccacc gaaacgcgtt cctgagaggt ccttccgtgc
     1501 tgtctcagga cttgcagatg gctgcgtttc cgcgcacttg ggtggcaggc cagttgtgcg
     1561 gcgttgacgg atacatggag tcgacggccc tcggcctcgt cgctgggctc aacgctgccg
     1621 ccctgttgcg aggcttttcg cggcctgcgt tgtccactca gactatgacg ggacttcttg
     1681 tggactatgt cacgcacgag agagaagact ttcagccaac gaatgccaac tatggacttc
     1741 ttcctctcga acccggtccc aagcagaagg cagtggaccg ggaagggttc gccgctgcgg
     1801 ccctcgaaca tactcgtcag gttgctgctg cggtccaggc atggacgtcg gagagcgagt
     1861 gacaccagac tcagggtctg tgcatggagg aggccaggac gtcgccacga gcttcgacgt
     1921 ggtggcagag atgaacgggt cgtacctgca gatgccggat gcggagtttc gagccaggat
     1981 ccgcacagcg tatcgtcgca tgtccgtctg cgacctgtgt ccccggcatt gtggggctca
     2041 ccggctgtcg ggagaggtcg gtcgctgtgg tgcctcccta aaacccaagg tctttgtatg
     2101 caatctgcat cgcggcgagg aacctcccgt cagcgggaca cgaggatcag ggaccgtatt
     2161 cttcagcggc tgcaacctgg agtgctgcta ctgccagaac tacccgctga gccagctgca
     2221 ccacgggaca gagatgtcgg ttggccgact cgcggctgcg ctcgtcgagt tgcagagaaa
     2281 aggagcacac aatatcaact tcgtcactcc ttcacaccag atacctcaga tgctcgccgc
     2341 cacattcgcc gcgcgacagc tcggactgca cgtgcccatc gtgtacaaca cgagcagcta
     2401 tgacgagcta tcggcgcttg cccttctgga aggggtcgta gacgtctacc tgggcgacct
     2461 ccgatatacg gacgaaagcg tcgggatgca actttcggga gtggacgact acgttcgtgt
     2521 tgccgagcgt gcactggttg agatgcatcg acaggtcgga gacatcctgc tcgacgacca
     2581 aggcaggtat gtgccgcgtg gactcatcgt tcggtacctg attcttccgc gccacacagt
     2641 catggccaga caggtctttg acttcgttct ccggcgcctc tcgagtcaga cctatgtgag
     2701 ccttatgacg cagtacttcc cggcatacaa ggcactcgac cggtcgctcg gcatttcgcg
     2761 aaagctcacc gacaccgagt atgatcgggt gatgagtgca tggctgcggt caggattgtc
     2821 tcacgggtgg gtacaggaca tggaggagga ggggagagcc tgaccgacag aagaggagtg
     2881 cgtccatcac gatgacccgc ccctcctttg cgcaagatgc gtcagatctt gctgactact
     2941 tcgcgagtaa tccccttgag gcattccatc acagaaacgc caccactcgc gatacccaag
     3001 tagacagcct tgttcacaag ctgcaagtcg ctgaccaatt cgtggaccgg catgatgccg
     3061 ggcatgtccc gcttgttcaa ctggtatatg attggagtat ctggcagctc gtactcctca
     3121 agattcttga acatgtccgc aaggttgttg atattgtcct ctcgccggtc acggtttgag
     3181 tccgctacga agacgatgcc gtcgacggac ttgagaacgg acttgcgtgt caacttgtac
     3241 agttcctgtc ccggaactgt gtagaggctc aggcggatct tgaaaccctt gactgtcccc
     3301 aactctacgg gcaagaagtc gaaaaagagc gtacgttccg tctcagtagc gatggaggtg
     3361 aactgtccct tcaccttctc cggaatcgtc cggaagatcc agctcagatt tgtagtcttg
     3421 ccgctcatcg cggggccata gtacacgacc ttgaacgtga tctcggattt cgaaaagtcg
     3481 atcagagaca tccagttcct cgcatcgata cactagaaga ggttgtcgat ctcgtcctgc
     3541 agacccttcc ggagatcctg ggaaggttct tgccgtggac cggacgccat cttttcgagc
     3601 accggcttga tcgaacccga gatcttcttg gcccagaacc tgatggcgcc aataggtgcc
     3661 ttgttgtcga atatgacggc aagcaaaatc aaggggtcca cgagcgaaat gtggatgttg
     3721 gtctctttgc cctggtgaaa cgtcagggtg aactcacgct ctccaagaag cttcgccagc
     3781 tcattcgtgg ctgagaatac gccggcagcg agggcagaca cagcaaccgc tgactcgcgt
     3841 gtcacggcgc cgctcttggc aaggagctcg ccactcttgt tgaggaggat tatgtccttg
     3901 gcctgcgaat ccacgagata ctgcgagaac agttcgtcaa tggtttcgat atcttccttc
     3961 gtgatcacca gtgaagacag ttggccttcc atctatgcct ccggttttga tacggtaacc
     4021 atcttcttgt ccgcaagagc gttgagagct gcccatgcac ggaagaaccc aaactcagct
     4081 tctttcaaga ctctgtcgaa ggagtctctc tcgcctgcct tctgcagcag aaggaactca
     4141 tcggcggtca gcgtcacggc cgcctcggag tcattctctg cgaggctcac ttgcagcccc
     4201 gacggttcgg tctgaacctc cgactgtctg tactcatcgg cgagccgggt cgcttctatc
     4261 aggagactcg tgttgctctc tgtgatcctt cgaggtggca tgataacgtt ggagaggaaa
     4321 ttgaacgtgc cttcgcgctc gagaaaggcg tcaaagaagg ccggtagacc gctgctgtag
     4381 ggcgactgcg catggatgac c
//
LOCUS       DATMZD010000097         2536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_33093,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000097 DATMZD010000000
VERSION     DATMZD010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33093"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1189
                     /locus_tag="VN478_02060"
     CDS             <1..1189
                     /locus_tag="VN478_02060"
                     /inference="COORDINATES: protein motif:HMM:NF019450.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HWQ21495.1"
                     /translation="NANNTGTITFTVGTVRVTVPVKAGFAPTPTVGFILSPQSMRVGE
                     GDEFALDLKARNLQMLFDHVDLTVAWDPALVALQGADASASLTGPIAPSTESGILRIQ
                     SDVTGLSGGETVLASLRFTAVAPTDKTGVNLRGTVSLGGAPVRVTTGGCAVAISPRFV
                     PPGDPGAPSAVGEQGQVRLTWSPGATGSFPVVRYDIYRKQGAADIETAELVGEAKPEA
                     TQFVDRGPLERATYYYWVMSEDERGNVSNPAGPAKAQPIIITDPITQVTLVFTVGKPA
                     VVMNGVTVPMETAPIVENGRTILPVRYVATPLGAQVLWDAKAQKVTLIGTRRVELWIG
                     KPTARVDGKDVPIDPANPKVVPRISNGRTMLPLRFVSEVFGAEILYDARLRTVTVTLS
                     KAT"
     gene            1201..1860
                     /locus_tag="VN478_02065"
     CDS             1201..1860
                     /locus_tag="VN478_02065"
                     /inference="COORDINATES: protein motif:HMM:TIGR01065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HWQ21496.1"
                     /translation="MVKFKEPFNGISHLMGGLAAVVVTVLLVRTAVIRGGIRASAPFL
                     VYGLSMVLLYSASAWYHLLDVSPRAHVMLRRLDHVSISILIAGSYTPVCLVALGGTLG
                     TWLCWIVWGLAGIVILTDIFWLDAPRGLKAACYVILGWVAVWAMAPLQRAIGWGGMAW
                     MLAGGLAYSVGALLYAIKKPNPWPGVLGFHEIWHLFVLAGSTGFVVLIWRYVLPLVKA
                     V"
     gene            1862..>2536
                     /locus_tag="VN478_02070"
     CDS             1862..>2536
                     /locus_tag="VN478_02070"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWQ21497.1"
                     /translation="MGLEFEALTPHLWSAKCSRLAMNTGVLHDRGHAALIDPALLPDE
                     VEDIAAFCDSRQLKVETVVITHHHWDHVLGAARFAGAHVTAHQAYIAQTALDLERTRC
                     SIARFYQAEGVSSAGSFDPPMPDQSVERIIGLMVGDVRVQLFQTPGHARDHLSAYDTE
                     SACLWAGDLLSDLEIPFVSDRLDAYERTLGMFAAMDVRILVPGHGSVERDRTEIAHRI
                     EADRSYL"
BASE COUNT          437 a          820 c          803 g          476 t
ORIGIN      
        1 gaacgccaac aacacgggca cgatcacatt caccgtcggc accgtcaggg tgaccgttcc
       61 agtgaaggcg gggttcgcac cgacgccgac cgtcgggttc attctgtcgc ctcaaagcat
      121 gcgggtcggc gagggggatg agttcgccct cgacctgaag gcgaggaacc tgcagatgct
      181 gttcgaccat gtggacctca ccgtagcgtg ggaccctgca ctcgtcgccc tgcagggcgc
      241 tgacgcatca gcctcgctga ccggccccat cgcgccgtcc acggagtcgg ggattctccg
      301 catccaaagc gacgtcactg gattgagtgg cggtgaaacg gtcctggcgt ccctgcgctt
      361 cacggccgtg gctccgaccg acaaaaccgg tgtgaacctg cgagggaccg tctctcttgg
      421 cggtgcacct gttcgtgtga cgactggcgg atgtgcagtc gccatcagtc cgcgcttcgt
      481 cccccctggg gaccccggcg ctccatcggc ggtcggggaa cagggccagg tccgcctcac
      541 gtggtcgccg ggagcgacgg gcagcttccc tgttgtgcgc tatgacatct accgcaagca
      601 gggtgcggcg gacatcgaaa cggctgaact agtgggagag gcaaagcccg aagcgacgca
      661 gttcgtcgac cgtggtccgc tcgaacgagc cacctactac tactgggtca tgtccgagga
      721 cgaacggggc aacgtcagca accctgcggg gcccgcaaag gcacagccga tcatcattac
      781 tgacccgatc acacaggtga ccctggtctt cacggtggga aagcctgcgg ttgtcatgaa
      841 tggcgtaacc gtcccgatgg agacagcgcc catcgtcgag aacggacgca ctatccttcc
      901 cgtcaggtac gtcgctacgc cgctgggggc ccaggtcctg tgggacgcca aggcgcagaa
      961 ggtcacgctc atcggtacca ggagggtcga gctgtggatc gggaagccga cagcacgagt
     1021 cgacgggaag gacgtcccca tcgatccagc caacccgaag gtcgtgcctc gcatcagcaa
     1081 cggcaggaca atgcttcctc tccgcttcgt cagcgaggtc tttggcgcgg agatcctcta
     1141 tgatgcgcgg ctgcgtaccg tgaccgtcac cctgagcaag gcgacctagg ggaaaccgcc
     1201 gtggtcaagt tcaaggagcc tttcaacggc atttcgcacc tcatgggagg cctcgctgcg
     1261 gtcgtcgtca ccgtgctgct tgtcaggacg gccgtcatcc gtggcggcat cagggcgagc
     1321 gctcccttcc tggtctacgg tctcagcatg gtcctcctgt attccgcgag tgcctggtac
     1381 cacctgctcg acgtcagccc gagggcgcac gtcatgctgc ggaggctcga ccacgtcagt
     1441 atctccatac tcatcgcggg ttcatatacg ccggtgtgtc tggtagcact ggggggcacg
     1501 ctcggaacct ggctctgctg gatcgtgtgg ggactggccg ggatcgtcat cctcaccgac
     1561 atcttctggc tcgacgctcc ccggggcctc aaggccgcct gctacgtcat tctcggctgg
     1621 gtcgccgttt gggcgatggc tccgcttcag cgtgccatcg gctggggcgg catggcctgg
     1681 atgctggcgg gaggccttgc ctacagcgtg ggcgccctgc tgtacgcgat caagaagccc
     1741 aatccgtggc cgggtgtcct aggcttccat gagatctggc acctgttcgt tctcgccggc
     1801 agcacgggct tcgtcgtgct gatctggcgc tatgtccttc ccctggtgaa ggccgtctga
     1861 catgggtctt gagttcgagg cgctgacgcc tcacctctgg tcggcgaaat gcagcagact
     1921 cgccatgaac acgggtgtcc tgcatgacag aggtcatgcg gcactcatcg acccggcgct
     1981 cctgcccgac gaggtggagg acatcgctgc cttctgcgac agccggcagc tgaaggtcga
     2041 gacggtcgtg atcacccacc accactggga ccatgttctg ggggccgccc gattcgctgg
     2101 ggcacacgtt acggcgcacc aggcgtatat cgcccagacg gccctcgatc ttgagcggac
     2161 ccgctgctcg attgcgcgct tctaccaggc agaaggagtg tcatctgccg gctcattcga
     2221 tccgccaatg ccggaccagt ctgtcgagcg catcatcggc ctcatggtcg gggacgtccg
     2281 cgtccagctc ttccagacac ccggccacgc gcgcgaccac ctctccgcgt acgacaccga
     2341 gtctgcatgt ctctgggcag gagacctcct gagcgacctc gagatcccgt tcgtgagcga
     2401 caggcttgat gcgtatgaga ggaccctagg catgttcgct gcaatggacg tccgcattct
     2461 ggttccgggt catggcagcg ttgaacgcga tcgcacagaa atcgcacacc gcatcgaggc
     2521 ggaccgctct tatctg
//
LOCUS       DATMZD010000098         2139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_33106,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000098 DATMZD010000000
VERSION     DATMZD010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33106"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1379
                     /locus_tag="VN478_02075"
     CDS             <1..1379
                     /locus_tag="VN478_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011461874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HWQ21498.1"
                     /translation="SIPVEKHPGDKVVGASINGNGSITFRATHVGADTVLAQIVKLVE
                     DAQGSKAPIAQLADVVSGYFVPIVFVIAVLSAAAWLIAGKGSVFALTIFISVLTIACT
                     CALGLATPTAIMVGTGKGAEYGVLIKGGAALEMAHKVQTVVFDKTGTITQGRPVVTDV
                     LTVPGVGRSEVLGLAASAEKNSEHPLGTAIVRAAQDENVALQPLEDFASVPGRGIEGT
                     VGSRHVLLGNAAFMGERSVDVAALAAAVDQFAEDGKTPMLVARDGMAVGVIAVADMVK
                     ESSARAIKTLHEMGIEVAMITGDNRRTAEAIGRQVGIDRVLAEVLPQDKALEVKKLQA
                     AGRRVAMVGDGINDAPALAQADVGIAIGNGTDVAIESADIVLMKSDLMDVPTAIQLSK
                     ATIRNIRQNLFWAFGYNTLGIPIAAGLLYAFGGPKLNPMIGAAAMSLSSVSVLTNALR
                     LRGFKPRR"
     gene            1406..1621
                     /locus_tag="VN478_02080"
     CDS             1406..1621
                     /locus_tag="VN478_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011461873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HWQ21499.1"
                     /translation="MKKLIQVKGMSCKHCVMHVTEALKEVPGVTDVVVSLEDGTATVD
                     TNAPVADEALAAAITDVGYTPGTVTDL"
     gene            1641..>2139
                     /locus_tag="VN478_02085"
     CDS             1641..>2139
                     /locus_tag="VN478_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21500.1"
                     /translation="MSATRILTVSLVLAAVVLGAAFMAPDAVAVYAVRSYDALLHAPG
                     VEIARADAAQAWVLTSPGGETLTFGGNDTAGSNQLVVTLDARPFLAAGLDRSALPAGV
                     ELDGSGASARLVMRYDKGAGTFSDGSDVQPLQALDDLVHNHRAMLGYHTALGHFGLAL
                     GGGNMV"
BASE COUNT          392 a          669 c          718 g          360 t
ORIGIN      
        1 agagcatccc ggtcgagaag catcccggcg acaaggtcgt gggcgcgagc atcaacggca
       61 acgggtccat caccttccgc gccacgcatg tcggagccga taccgtgctg gcccagatcg
      121 tcaagctggt cgaagacgca cagggcagca aggcgccgat cgcgcagctg gccgacgtcg
      181 tgtcaggata cttcgtcccc atcgtcttcg tcatcgccgt cctgtctgcg gcggcatggc
      241 tcattgccgg caaggggagc gtgtttgcct tgaccatctt catctccgtc ctgacgatcg
      301 cgtgcacgtg tgcacttgga ctcgcgacgc cgaccgccat catggtcggg acaggcaagg
      361 gtgcagagta tggcgtactc atcaagggcg gcgctgcgct ggaaatggcg cacaaggtgc
      421 agaccgtcgt gttcgacaag acgggcacca tcacgcaggg caggccggtc gtaaccgacg
      481 tcctgacggt tcccggcgtg ggacgcagtg aagtccttgg cctggcggcc tccgccgaga
      541 agaactcgga gcacccgctc gggactgcga tcgtcagggc ggcccaggac gagaacgtcg
      601 cgctgcagcc cctggaagac ttcgcgtcgg tcccgggacg gggcatcgag gggaccgttg
      661 gttccaggca tgtgctgctg ggcaacgccg ccttcatggg cgagcgttcg gtggatgtcg
      721 cggctcttgc tgctgccgtg gaccagttcg ccgaagacgg caagaccccc atgctggtcg
      781 ctcgcgacgg tatggccgtc ggcgtcatcg ccgtggcgga catggtcaag gagagcagcg
      841 cccgcgccat caagaccctg catgaaatgg gtatcgaggt cgcgatgatc acaggggaca
      901 accggaggac ggcggaagcc atcgggcgac aggtcggcat cgatcgcgtc ctggccgagg
      961 tcctgccaca ggacaaggcc ctggaagtga agaagctgca ggccgccggc agacgggtgg
     1021 ccatggtcgg cgacggcatc aacgacgctc cggcgcttgc acaggcggat gtcggcatcg
     1081 cgatcggcaa cgggacggac gtagccatcg agtcggcgga catcgtcctc atgaagagcg
     1141 acctcatgga cgtgccgacg gctatccagc tcagcaaggc gacgatccgc aacatccggc
     1201 agaacctgtt ctgggcattt ggctacaaca cgctgggcat ccccattgcc gccggactgc
     1261 tgtatgcgtt tggcggccca aagctcaacc cgatgatcgg cgcggcggcg atgtcgctgt
     1321 cgtctgtctc ggtcctgacg aacgcactca gactgcgcgg cttcaagcct cgcaggtagt
     1381 attcgcacga caacggagga atcacatgaa gaaactgatc caggtcaagg gtatgagctg
     1441 caagcactgt gtcatgcacg tcactgaggc actgaaagaa gtaccggggg tcacggacgt
     1501 cgtcgtcagc ctggaagatg gtactgcgac ggtcgacacc aatgctccag tagccgatga
     1561 ggcgcttgcg gcagccatca cagacgttgg ctacacgccc ggcacggtca cggatctgta
     1621 ggccgggagc aaagacggag atgagcgcca caaggatcct taccgtgtct ctcgtgcttg
     1681 ccgcggtcgt gctcggtgcg gcattcatgg cgccggatgc tgtcgccgtc tacgcagtcc
     1741 gttcgtacga tgccctgctc cacgcccccg gcgtggagat cgcgcgtgcc gacgctgctc
     1801 aagcctgggt gctcacgtcg cccggcggag aaacgctgac ctttgggggc aacgatacgg
     1861 ccggctcgaa ccagctggtc gtcacgctgg acgcccgtcc cttcctcgct gccggcctcg
     1921 atcggtctgc gcttcctgcg ggagtggaac ttgacggcag cggggcgtcc gcgcgcctgg
     1981 tcatgcggta cgacaagggc gcaggaacgt tcagcgacgg ttccgacgta cagcccctgc
     2041 aggccctgga tgacctcgtt cacaaccacc gtgcgatgct gggctaccat accgcgctcg
     2101 gacacttcgg gctggcgctg ggcgggggca acatggtcg
//
LOCUS       DATMZD010000099         2406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_63048,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000099 DATMZD010000000
VERSION     DATMZD010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63048"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..879)
                     /locus_tag="VN478_02090"
     CDS             complement(<1..879)
                     /locus_tag="VN478_02090"
                     /inference="COORDINATES: protein motif:HMM:NF017181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family peptidase"
                     /protein_id="HWQ21501.1"
                     /translation="MEQTSIELLKKLTEARGISGREDEVAAILAGELESHVDRLERDS
                     IKNLFAVKGMDKPGPNVMLNAHMDEVGLYVTRITKEGYLHVGKAGSIDDRLLLGKKVL
                     VGRDRLPGVTAIKAVHLSTHEEVENTVIKVDDIVVDVGAKSDKELVGKLAIGDQITFD
                     TAFGTFGDGYLIGKAFDDRVGCAVVTELLKMEWPFPVIGMFSTQEEVGTKGARVGAWR
                     YPPDISITVEGTIAADIGEVKDYQEVSTLGAGTVLTIKDGGMITDHRLRNQLIEVAKT
                     NGLPWQFKRMPAGGTDA"
     gene            complement(860..1909)
                     /locus_tag="VN478_02095"
     CDS             complement(860..1909)
                     /locus_tag="VN478_02095"
                     /inference="COORDINATES: protein motif:HMM:NF017181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HWQ21502.1"
                     /translation="MHKLVEQLTSAYAVSGDEHRLHDILRSALDGIALEIHDTPLGNL
                     IAVRKGRSSEHSILFAAHLDEVGAWVTRINDSGFLGIHCRSIDSRVWPGLQVVVHGTH
                     PLPGVIGLKPVHVMSHEEMDKPVVPDELWIDVGLSREQVLKLVPIGSTVTFTAGISRL
                     MNERFAGKTQDDRLCAVALVDALERMRDRTLPYDVVMAFTVQEEITGNGAWTSGYFAH
                     PDVAIAVDVTHGDTDGVPDHKTIPLGGGPGVAVGPVVNDHVLDLIRRSAKAVEIPTSE
                     ESCMSYSGTDADELQLVGSGIATGVLSIPLRYMHSPVETADQVDVERTARLIAEVATG
                     FDGTFIGELYGTDKH"
     gene            2021..>2406
                     /locus_tag="VN478_02100"
     CDS             2021..>2406
                     /locus_tag="VN478_02100"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013458762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HWQ21503.1"
                     /translation="MRRQAGTVPHESPWNRHIATVVAAIALTAAMFVSAFVMARIPAA
                     AAPVAQMEVAITGADRVLVVSPHPDDESIACSGLIQRALEVGAQVRVLWMTAGDHNIV
                     GPQLFWRRVAVTPAQFRDIGHRRMQEA"
BASE COUNT          448 a          744 c          720 g          494 t
ORIGIN      
        1 ggcatcggtg cctcctgccg gcatgcgctt gaattgccag ggcagcccat tcgtcttcgc
       61 gacctcgatc agctggttgc gcagccggtg gtccgtgatc atgcccccat ccttgatggt
      121 gaggacagtg ccggcgccca atgtcgagac ctcctggtag tctttgactt cgccgatgtc
      181 ggccgcgatc gtgccctcta ccgtgatgct gatgtcgggg gggtagcgcc acgcgccgac
      241 cctggcaccc ttggtcccca cctcttcctg tgtcgagaac atcccgatga cggggaaggg
      301 ccactccatc ttgagcagtt cggtgacgac ggcacagccg acgcggtcgt caaaggcctt
      361 accgatcagg tacccatcgc caaacgttcc aaacgcagtg tcgaaggtga tctgatcgcc
      421 gatggcgagc tttcccacca gttccttgtc ggacttggca ccgacgtcga cgacgatgtc
      481 gtccaccttg atgacagtgt tctcgacttc ctcatgggtc gacaggtgca cggccttgat
      541 ggcggtcacg ccgggaaggc gatctctgcc gaccagcacc ttcttgccca gcagaagcct
      601 gtcatcgatg ctgccggcct tgccgacatg caggtatcct tccttggtga ttcgggtcac
      661 gtacaggcca acctcgtcca tatgtgcgtt cagcatgacg ttgggccccg gcttgtccat
      721 gcccttgact gcaaacaggt tcttgatgga gtcccgctcg aggcggtcga cgtgactctc
      781 cagttcaccc gcgagaatgg cggcgacctc gtcctcgcgc cccgagatcc cgcgggcttc
      841 cgtgagcttc ttcagcagct caatgcttgt ctgttccata cagctctcct atgaacgttc
      901 cgtcgaagcc cgtcgcgacc tcggcgatca atctcgccgt cctttcgaca tctacctgat
      961 cggccgtctc aacgggtgag tgcatgtatc gcagaggaat gctgaggacg cccgtggcga
     1021 taccggagcc gaccagctgc aactcatcgg cgtccgtgcc cgaataggac atgcaggatt
     1081 cttcactggt ggggatctcg acggccttgg cgctcctgcg gatgagatcg aggacatgat
     1141 cgttgaccac gggtccaact gcaacgccgg gaccgccgcc cagcggaatt gtcttgtggt
     1201 ccggaacccc gtcggtgtca ccgtgggtga cgtcgacagc gatcgcgacg tccggatgtg
     1261 cgaaataccc actggtccag gcgccgttgc cggtgatctc ctcctggaca gtgaacgcca
     1321 tcacgacgtc gtacggcaac gtcctgtccc gcattcgttc gagcgcatcg accaaggcca
     1381 cggcacagag ccggtcatcc tgggtcttcc ccgcgaagcg ctcattcatg agtctgctga
     1441 tgccggcggt gaatgtcacg gtagaaccga tgggaaccaa cttgaggacc tgctcacgtg
     1501 acagcccgac gtcgatccag agttcatcag ggacgaccgg cttgtccatt tcctcgtgcg
     1561 acatgacgtg gaccggcttg agcccgatga cgccaggcaa cggatgagta ccgtggacga
     1621 cgacctggag gcccggccag acgcggctgt cgatggatcg gcaatggatc ccgaggaagc
     1681 cactgtcgtt gattcgtgtg acccaggcgc caacctcgtc gagatgagcg gcgaagagga
     1741 tagagtgttc gctcgagcgg cccttgcgca cggcgatgag attgccaagg ggtgtgtcat
     1801 ggatctcgag ggcaatgccg tcgagtgcgc tgcgcaggat gtcatgaagc ctgtgctcgt
     1861 ctcctgaaac cgcataggcg cttgtcaact gctctacaag cttgtgcatg gtcatctcct
     1921 ctcccgtaac cagactattg tacggcaaac gaacagaacg tcaaaaagcg tcctggcaga
     1981 tcgattgacc gcgtcactca cctcactaga atagactgca atgagaagac aggctggcac
     2041 ggtaccacac gaaagccctt ggaaccgaca catcgccacg gtagtagctg caatcgcgct
     2101 caccgcggcg atgtttgttt cggcattcgt catggctcgc atcccggctg cggccgcgcc
     2161 ggttgcccag atggaagtgg cgataacggg cgcagaccgc gtgcttgtcg tttctcctca
     2221 tccggatgat gagtccattg catgttccgg cctcatccag cgtgctctgg aggtgggcgc
     2281 gcaagtccgt gtcctgtgga tgacggcagg cgatcacaac atcgtgggtc cgcagctgtt
     2341 ctggcggaga gtagcggtca ctcccgcgca gttccgtgac atcggacaca ggaggatgca
     2401 ggaagc
//
LOCUS       DATMZD010000100         2190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_93571,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000100 DATMZD010000000
VERSION     DATMZD010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93571"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="VN478_02105"
     CDS             <1..732
                     /locus_tag="VN478_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21504.1"
                     /translation="YFTLKNDLSHIYSCSQTSKPIVVLTVTGSIGMFSVSPDRRFAAV
                     AMKEAGSARQQVSILDIWSLRDKKVISTIKSDDRTGYYDQSPGWGDQGWWVVAHMSQS
                     PSPVLTYIFTSYTSFMPGVSYFVQASFSDSAPGHVSVARYPASEYIPLDNETGSIGVA
                     FDPTTNTVAYAGWDQFPDAGTAAPKLFLYNIISGSAHSIRTLPLTTLLPVWIGPDTLE
                     YSTTLGLRGTSTANWVTLYDVLKPH"
     gene            830..>2190
                     /locus_tag="VN478_02110"
     CDS             830..>2190
                     /locus_tag="VN478_02110"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HWQ21505.1"
                     /translation="MNRNGRSGERKWVIAGLLVLALCSTALTGCNRSTVPNAGDGPTS
                     GTVNSRFVDVDGLLTAPTRVVMSYPTEGLYWDLGAPQFLDGMIYTLSYKAGNENAWMT
                     AWNQTTGERLWSVDTGLRWIWSFVSDGEHLFLHTANTFRCLDKMTGETFWESTPTPAS
                     PFVFDLSREIVLHINKQNNVADRLYVLGAETKTLDTGNEQHNPGIYILDGANGKLLGR
                     MDWPALTLSSGRGELLCDGTTLYASMPESTKAEYPAQKSSLVAFDLTTNKVLWQESVD
                     GEGGNLVKQGDMMVFIRSAAFIDDWIDVWQTDNTTASATRLWTRKIEEKQGAYHVFPF
                     AVTNAFVYLQGEQGTLMALDLASGKEVWDHQFAPIKEPITEGPDMGKEHDTYQLMTMT
                     TARDALYVQDGGGLLAVFDPASGKELWSKRISQVVQKQTYVAGAYVMQVVDEGIAVIM
                     SNGT"
BASE COUNT          508 a          582 c          639 g          461 t
ORIGIN      
        1 tatttcactc tcaagaatga cttgtcgcat atttattcct gcagccagac gtcgaagccc
       61 atcgtcgtcc ttaccgttac cggcagcatc ggtatgttct ccgtgtctcc ggacaggcgc
      121 tttgctgccg tcgccatgaa agaagctggg agcgccaggc aacaggtcag catattggac
      181 atctggtctc tcagagacaa gaaggtcatc tctacgatca aatccgacga tcgtacgggc
      241 tactacgacc agagtccggg atggggagat cagggttggt gggtcgtggc acacatgtcg
      301 caaagcccat cccctgtctt gacctacatc tttacgtcat acacgagttt catgccgggc
      361 gtctcctact tcgtccaggc ttcctttagc gacagtgcac ccggccacgt atcggtcgcg
      421 cgatatccgg ctagcgagta cattcctctc gacaatgaga ctggcagcat cggcgtcgct
      481 tttgatccga cgaccaatac ggtagcatat gctggatggg accagtttcc cgacgctgga
      541 acggctgcgc cgaagctgtt cctgtacaac atcatttccg gaagcgctca cagtatacgt
      601 acgcttccgc tcacgacgct gctacctgtc tggatcggtc cggacacgct cgaatacagc
      661 acgaccctgg ggctgcgggg caccagtact gcgaactggg tcacgctgta tgacgtactg
      721 aagccccatt gataatgcaa tgccgcaatg atgcggcgcg ctgatggcgc tggtatgatc
      781 agatactgct ccaacgccct tgttgaagga acgtattgga gggaacaaag tgaataggaa
      841 cggacgatca ggcgaacgaa aatgggtcat cgcgggattg ctcgtgctgg cgctgtgcag
      901 taccgccttg acaggctgta acagatcgac cgtgcccaat gccggcgatg gcccaacgtc
      961 aggcacggtc aacagcaggt ttgttgacgt ggacggcctc ctgacggcac ccacgagagt
     1021 cgtcatgtca taccctaccg aagggctgta ctgggacctc ggggctccac agttcctgga
     1081 cggcatgatc tacaccttgt catacaaagc tggcaacgag aacgcatgga tgacagcatg
     1141 gaatcagaca acgggagaga gactctggtc ggtagacacg ggtctgcgct ggatttggtc
     1201 gtttgtgtcc gatggggaac acctgttcct gcatacggcc aacaccttcc gttgtctgga
     1261 caaaatgacg ggggagactt tctgggaaag tactccaacg cctgcttcgc ctttcgtgtt
     1321 cgacctgagc agggagatcg tgctgcatat caacaagcag aacaacgtgg cagaccgtct
     1381 ctacgttctt ggcgcagaga cgaagacact ggatacggga aacgagcaac acaatccagg
     1441 catctacatc cttgatggtg ccaacggaaa gctgctgggt cgcatggatt ggcctgcact
     1501 cacgctgtct agtggcaggg gagaactgtt gtgcgatggc acgacgctgt atgcaagtat
     1561 gccggagtcg accaaagctg aatatccagc ccagaagtcc tctcttgtgg cgtttgacct
     1621 gacgacgaac aaggtcctgt ggcaagagag cgtcgatgga gagggcggca atctcgttaa
     1681 gcagggcgac atgatggttt tcattcgcag tgctgctttc atcgatgatt ggatcgacgt
     1741 atggcagaca gacaatacaa cggcgtccgc cacgcggctc tggacacgga agattgagga
     1801 gaaacagggt gcgtaccatg tcttcccgtt cgcggtcacc aatgcgtttg tctaccttca
     1861 gggtgagcaa ggtacactca tggcgctgga ccttgccagc ggcaaagaag tatgggacca
     1921 tcagtttgcc ccgatcaaag aacccataac agagggtcct gatatgggaa aagagcacga
     1981 cacgtaccag ctcatgacga tgacaacggc gagggacgct ctgtatgttc aggacggcgg
     2041 cggactgctg gcagtcttcg atcctgcaag cggcaaggag ctgtggagca agcgcatctc
     2101 gcaggtagtc cagaagcaga cttatgtcgc cggtgcgtac gtgatgcagg tagttgacga
     2161 gggaattgcc gtgatcatgt cgaacgggac
//
LOCUS       DATMZD010000101         5912 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_134068,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000101 DATMZD010000000
VERSION     DATMZD010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5912
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134068"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..1004)
                     /locus_tag="VN478_02115"
     CDS             complement(57..1004)
                     /locus_tag="VN478_02115"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HWQ21506.1"
                     /translation="MDSEYNPIKIFSGNANKPLAEKVASYLGIPLGQAEVGRFPDGEI
                     KLRINENVRGLNAYVIQPTNAPAENLFELLILLDALKRASAASITAIIPFFGYARQDR
                     KVRSREPISAKLVANLVTVAGADRVVAIDLHAAQIQGFFDIPTDNLTAMVQFAQYIKR
                     KDLEDIVVLSPDVGGVARSSTLAQTIHASQALFVKKRLSPDAVEIQSLIGDVRGKNVI
                     IADDAIHTGNTMLNACDRALAEGAKSVIACATHAVFAGDSLGLFARSNFSEVIVTDTM
                     FVPGREYPSMFTVLSVSELIAKAIYSIHNHSSVSALFQL"
     gene            complement(994..2370)
                     /gene="glmU"
                     /locus_tag="VN478_02120"
     CDS             complement(994..2370)
                     /gene="glmU"
                     /locus_tag="VN478_02120"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HWQ21507.1"
                     /translation="MSTTAVQAMILAGGLGTRMRSAVPKVLHDILGHPMLWYVVEALH
                     DIDAQPIVVTPGANEAFRAVFGNRLTYAVQAEQHGSGHAVQCGMEHVTAEFVLVCNGD
                     DPLPSAEDYGEFLEDSLACRSDAAVLAACLRDTGQLGRVVRTADGTFVSIVEARDATP
                     AQLTIREVNTGVYLFRSDELRRWLTQMKPNNAQGEYYITDCLPMAIRDGKNVCCHVAH
                     GSWEMSGVNDRVELAMAASLLQRRKLEHLQRAGVTVDMPETVRIDWDVIVGRDSEIRQ
                     GSCLRGSTRVGVSSIIGPNTTLVDTEVGDETTVLASIAEGSTIGSNCSVGPFSYLRPG
                     TVTAERSKVGAFCELKASSLGAGSKIPHLSYVGDATIAENVNVGAGTITCNYDGFTHT
                     KHHTTIEKDVFIGANNNLVAPVTIHEGAYTATGSTITHDVPPEALGIARAVQVDKEGW
                     VRRKKHGQ"
     gene            complement(2378..3568)
                     /locus_tag="VN478_02125"
     CDS             complement(2378..3568)
                     /locus_tag="VN478_02125"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HWQ21508.1"
                     /translation="MNERFQATMQAELDRLEKEGLSLRIRTLDSEQGPACTIDGKYVV
                     NLAANNYLGIIARKDVKEAAKRAIDEFGVGSGAVKTIIGNTRLIVDCEKKLAKFKDVE
                     DVLMYQGGLTANSGTIPALVHDGDFIFSDELNHASIIDGCRLGKAKIVPFKHCDVASL
                     EERIKEYESEPGTKMVITDGVFSMDGDIAPLPDIVEVAKKHDVVTMVDDAHGEGVLGD
                     HGRGIVNHFHLEGEVDVEIGTMSKAFGVVGGYAAGTHTLVEYLKNKSRTILFSSTITP
                     ADAGAILKVIDILTESDDLVHKLWENAAYFKAKMKAYGFDTWRSETPITPIIVGDGQL
                     AKNLSVRLFEKGVFAQSLGFPTVPKGLARIRCMLCSTHTKEQLDFAADMFHESALELG
                     ILKS"
     gene            complement(3573..4622)
                     /gene="tdh"
                     /locus_tag="VN478_02130"
     CDS             complement(3573..4622)
                     /gene="tdh"
                     /locus_tag="VN478_02130"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HWQ21509.1"
                     /translation="MTGTMLALVKERPERGATLTEVNIPLIADDEVLVKIDYASICGT
                     DVHIYVWNEWAQNRIKTPHIMGHEFSGHVVETGRSVRGFQAGDFVSSETHIYCGHCYQ
                     CHTGHSEVCQNLQILGVDRAGAFAEYIAVPERVLWKNDQSIPAAWASIQEPMGNALDT
                     VNSESVAGKTVLVTGAGPIGVLAVGIAKAFGATQLFVSDLSDYRLDLAKSMGADFVLN
                     PGHDDIESVVREQTHGIGVDVVLEMSGSEKAMHQGLKALTPGGRMSMLGLPERPVTID
                     LTNEVIFKEIRIYGITGRKIFSTWQTVSRLLASRIVDPSPAITHQLKFEDWQTGMDAM
                     VAGTCGKVVLQIAGR"
     gene            complement(4619..5257)
                     /gene="thyX"
                     /locus_tag="VN478_02135"
     CDS             complement(4619..5257)
                     /gene="thyX"
                     /locus_tag="VN478_02135"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018963147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HWQ21510.1"
                     /translation="MRVELLAYTPDADMLCAYAAHICYSSAPFDAERSAGILRKVRAN
                     GHLSVMEHASFSFLVEGLSRAASHQLVRHRLASYSQQSLRFTKAQPVFVSPPGHDFGR
                     FYEQAYAEYLRLLSEGVHEEDARYVLPQGVASRLVVTMNARELRHFFELRCCLRAQFE
                     IRALAWLMLAKAKAVAPLLLEDAGPICLQGMECPEQSFACHLKTKGQKGDSE"
     gene            complement(5254..>5912)
                     /locus_tag="VN478_02140"
     CDS             complement(5254..>5912)
                     /locus_tag="VN478_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MTAP family purine nucleoside phosphorylase"
                     /protein_id="HWQ21511.1"
                     /translation="VCVLKRHGEGHVLPPHLINYRANIMALYMLGVEKVVTTSAVGSL
                     KKSIAPGSYALMDGFLDFTKVRAQTFFETGRVIHTDMTHPYSAAITEVLATEMTAIGL
                     SLHTGAVYVAAEGPRFESPQEVRMYATLGGSVVGMTGLPEVSLAREAGLEYQTIAIIT
                     NYAAGIGSSLLTHKEVLDAMGAASASLIEVLRRSLPGLVQLPKDKGDDEHPLDFLLGS
                     "
BASE COUNT         1223 a         1770 c         1649 g         1270 t
ORIGIN      
        1 atctttgaca ttgtatgcca aatcagctct gaatcaagta gacagccttc agagtgtcac
       61 aattggaaca gggcggagac cgaggagtgg ttgtgtatgc tgtagatagc tttggcgata
      121 agctccgata ctgacaagac cgtgaacatc gaagggtact cgcgtccagg tacgaacatc
      181 gtatctgtca caatcacttc gctgaagttt gaacgggcaa agagtccgag agagtcccct
      241 gcaaacactg catgcgttgc acaggcaatt acgctcttgg caccctcggc cagagcgcgg
      301 tcgcaggcgt tcaacatggt gtttcccgta tgaatcgcat cgtcggcaat gatgacattc
      361 ttccctcgca catcgcctat gagcgactga atctcaaccg catcaggtga gaggcgcttc
      421 ttcacgaaca aggcctgtga ggcgtgaatc gtctgggcga gtgtgctgga gcgcgcaaca
      481 cccccgacat cgggagacag gaccacgatg tcctcgaggt ccttgcgctt gatgtactgc
      541 gcaaactgga ccatagccgt gaggttgtca gtcgggatgt cgaagaagcc ctggatctga
      601 gcggcgtgca gatcgatcgc tacgacacgg tcagctcctg cgacggtaac gagattggca
      661 accagcttgg cgctgatggg ctcccgcgag cgaaccttac ggtcctgacg cgcgtacccg
      721 aagaagggga tgattgccgt gatactcgct gctgaagcac gtttcagggc gtccagcagg
      781 atcagcagct caaacaggtt ttctgcagga gcgttagtgg gctggatgac atatgcattc
      841 agccctcgca cgttctcgtt gatgcgcagc ttgatctcgc cgtcgggaaa acggccgacc
      901 tcggcctgtc caaggggaat gccgagatag gacgcaacct tctcagccaa tggcttgttc
      961 gcattgccag agaagatctt gatcgggttg tattcactgt ccatgctttt tcctcctgac
     1021 ccatccctct ttgtcgacct gaaccgctcg tgcgatacca agagcctctg ggggcacatc
     1081 atgggtgatt gtcgacccgg tcgccgtgta cgctccttca tggatcgtca ctggagcgac
     1141 caggttgttg ttggcgccga tgaagacgtc tttctcaata gtcgtgtggt gcttcgtatg
     1201 cgtgaagcca tcatagttgc aggtgatcgt gccagctccg acgttgacgt tctcagcgat
     1261 cgtggcgtca ccgacgtatg agagatgcgg aatcttgctc cccgccccca acgacgaggc
     1321 tttgagttcg cagaacgcac cgaccttcga ccgctcagcg gtcacagtcc cgggccgaag
     1381 gtaggagaag gggccgacgc tgcagttgct gccgatggtg gaaccttcag cgatcgatgc
     1441 cagcactgtg gtctcgtcgc cgacctctgt gtcgacaagc gtcgtgttgg gaccgatgat
     1501 actggagact ccgaccctgg tgcttcccct cagacaagac ccctgccgaa tctctgagtc
     1561 acggccgaca atcacgtccc agtcgatacg aactgtctct ggcatgtcaa cggtgactcc
     1621 agcccgctgc aggtgttcca gcttccgacg ctgaagcagg ctggcagcca tcgcaagttc
     1681 gacccgatca tttaccccgg acatctccca cgaaccatgc gccacgtggc aacacacgtt
     1741 cttgccgtcc cgtatggcca tcggcaggca atcggttatg tagtactcgc cctgggcgtt
     1801 gttcggtttc atctgtgtca gccatctgcg aagttcgtct gaacggaaca ggtagacgcc
     1861 ggtgttgacc tcacggatag tcagttgcgc tggcgtcgca tcccgggcct ccacgatgct
     1921 cacaaacgtc ccgtccgcgg tacgcacgac acgtcccagt tgaccagtgt ctctgagaca
     1981 cgctgccagg acggccgcgt ccgagcgaca ggcaaggctg tcctccagga actcgccgta
     2041 gtcctctgct gagggcaagg gatcatcacc gttgcatacg agaacaaact ccgcagtaac
     2101 gtgctccata ccgcactgga cggcgtgacc gctgccgtgc tgctcagcct gaaccgcata
     2161 ggtcaggcga ttgccaaaga ccgcgcgaaa ggcttcattg gcaccgggag tgaccacaat
     2221 gggctgggcg tcgatgtcgt gaagcgcctc aaccacgtac cacagcatgg gatggccaag
     2281 gatgtcgtga aggaccttcg gcaccgcact tctcatgcgt gtgccgagtc ctccggccag
     2341 gatcattgct tggacagcgg ttgtcgacat tggacgacta gctcttcagg atgcccagct
     2401 caagggcgga ctcatggaac atgtcggcag cgaagtccag ttgttccttc gtgtgcgtcg
     2461 agcataacat gcagcgaatg cgcgcaagcc cctttgggac tgtcgggaag ccgagcgact
     2521 gggcaaagac gcccttctcg aacagcctga ccgagagatt cttggccagc tgtccatcgc
     2581 cgacaatgat cggggtgatc ggagtctcgc tgcgccaggt gtcgaacccg taggccttca
     2641 tcttcgcctt gaagtatgct gcattctccc acagcttgtg gaccaggtca tcgctctcgg
     2701 tcagaatgtc gatgaccttc aggatcgcac cggcatcggc cggagtgatc gtggagctga
     2761 acaggatcgt gcgggacttg ttcttcagat actcgacgag cgtatgcgtg cccgcagcgt
     2821 atccacccac tacgccaaat gctttcgaca tcgttcctat ctcgacatcg acttcacctt
     2881 cgagatggaa atggttcacg atcccacgtc cgtggtctcc caggacgccc tcgccatgcg
     2941 catcgtcgac catcgtcacc acgtcatgct tcttggcgac ttcgacgata tccggcagag
     3001 gagctatgtc gccgtccatg ctgaagacgc catccgttat gaccatcttc gtgccaggtt
     3061 cactctcgta ctccttgatc ctctcttcaa ggcttgcgac gtcgcaatgc ttgaagggca
     3121 cgatcttggc cttgcctaga cgacacccgt cgatgatgct ggcatggttg agctcgtcgc
     3181 tgaagatgaa gtctccgtcg tggacaagtg ctgggatggt gccggagttt gcggtgagac
     3241 ctccctggta catgagaacg tcctcgacat ccttgaactt cgccagcttc ttctcgcaat
     3301 cgacgatgag acgcgtgttg ccgatgatcg tcttgaccgc accggagcct acgccgaact
     3361 cgtcaatggc cctcttggca gcctccttga catccttgcg ggcaatgatg cccaggtaat
     3421 tgttggcggc aaggttcacg acatacttgc cgtcgatcgt acatgctgga ccctgctcgg
     3481 agtcgagcgt ccgtatcctg aggctgagcc cttccttctc aagcctgtca agctcggcct
     3541 gcatggtagc ctggaatctc tcgttcatgg catcacctcc ccgcgatctg aagaacgacc
     3601 ttgccacacg tcccagcgac catggcatcc atgcccgtct gccagtcctc gaacttcagt
     3661 tgatgcgtga tggcaggact cgggtctaca atgcgcgaag cgagcagacg cgataccgtc
     3721 tgccaagtcg agaagatctt gcgacctgtt atgccataga tgcgaatctc tttgaagatg
     3781 acctcattgg tcaagtcaat ggtcaccgga cgctcaggga gtccaagcat cgacatgcgt
     3841 ccgccaggtg tcagtgcttt caagccctgg tgcatggctt tctcactgcc tgacatctcc
     3901 agcacgacgt cgactccgat accatgcgtc tgctctctga ccactgactc gatatcgtca
     3961 tgaccgggat tgagcacgaa atcggcaccc atgctcttgg caaggtcaag tcgatagtcc
     4021 gaaagatcgc tcacgaagag ctgtgtggct ccgaaagcct tggcaatccc gacggcgagc
     4081 actccgatcg gacctgcccc cgtcaccaga acggtctttc ccgctaccga ctcagaattc
     4141 acagtatcca gggcgttccc catcggctcc tggatcgaag cccacgcagc tggaatcgac
     4201 tgatcgttct tccagagcac gcgttcggga acggcgatat actccgcaaa cgctccggcc
     4261 cggtccacac ccagaatctg gaggttctgg cagacctcac tgtggccagt gtggcattgg
     4321 tagcagtgcc cgcagtagat gtgtgtctcg gaactgacga agtcacccgc ctggaatccc
     4381 cgcaccgacc tgcccgtctc tacgacgtgt ccggagaact cgtggcccat gatgtgcgga
     4441 gtcttgatcc ggttctgtgc ccactcgttc cacacgtaga tatggacgtc tgtcccacag
     4501 atagaggcgt agtcgatctt gaccagcacc tcgtcgtctg cgatcaacgg aatattcacc
     4561 tccgtcaacg tcgcccctcg ctccgggcgt tccttcacaa gcgcgagcat tgttcctgtc
     4621 attccgagtc tcctttctgt cccttcgttt tcaagtgaca cgcgaacgat tgctccgggc
     4681 actccattcc ctgcaggcat atcgggccag catcttccaa caggaggggt gcaacggctt
     4741 tggcttttgc cagcataagc cacgccaggg cacgaatctc gaactgtgct ctcaggcaac
     4801 accgcagctc gaagaagtgt ctcagttccc tggcattcat cgtgaccacc agacgcgacg
     4861 cgacaccctg tggaaggacg tagcgagcgt cttcttcatg cactccctct gagagcagac
     4921 gcaggtattc cgcataggcc tgctcgtaga agcgtccgaa atcatgtcct ggcggagaga
     4981 cgaagaccgg ttgggccttc gtgaaccgca ggctctgctg tgagtacgag gccagccgat
     5041 gtctcacgag ctgatgagaa gctgcacgcg acagcccttc gacgagaaac gagaagctcg
     5101 catgctccat gaccgagaga tgtccgttgg cgcgcacctt ccgcagaatg ccggcgcttc
     5161 gctccgcatc aaatggtgcc gaactgtagc agatgtgagc cgcataggca cacagcatgt
     5221 ccgcatcagg agtgtaagcc agcagttcta ctctcatgat cccaacagga agtcgagtgg
     5281 atgttcgtcg tcacccttgt ccttcggaag ctggacaaga ccgggaagag accgacgcaa
     5341 gacctcgatc agggatgccg acgctgcacc catggcgtcg agaacctcct tgtgcgtcag
     5401 cagtgacgag cctatgccgg ccgcatagtt tgtgatgatg gcaattgtct gatactcgag
     5461 gcctgcttcc cgggcaagag atacctcagg cagacccgtc attccgacca ccgatccgcc
     5521 caaggtcgca tacatgcgca cttcctgcgg cgactcgaag cgtggacctt ctgcagcgac
     5581 gtagactgcg cccgtgtgaa gcgagaggcc gatggcagtc atctcagtcg ccaatacctc
     5641 agtgatggca gcgctgtagg gatgagtcat gtccgtgtga atgactctgc cagtctcaaa
     5701 gaaggtctgg gcgcgcactt tggtgaaatc caggaagccg tccatcaggg cgtacgaccc
     5761 aggagcgatc gacttcttga gcgaccccac ggcactcgtc gtaacgactt tctcgacacc
     5821 aagcatgtag agcgccatga tgttggctcg gtagttgatg agatgaggag gcaggacgtg
     5881 cccttcgcca tgacgtttga gaacgcagac tt
//
LOCUS       DATMZD010000102        11807 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_143556,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000102 DATMZD010000000
VERSION     DATMZD010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 11807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11807
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143556"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="VN478_02145"
     CDS             complement(<1..581)
                     /locus_tag="VN478_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008192716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HWQ21512.1"
                     /translation="MSNDERFSTLRSKREQIVAGGGPGRVKKQHEAGKLTARERIELL
                     LDPGSFQEENVFVSHRNTGFGLDKQELPGDGVVTGHGYVGARLVFVYSQDFTVAGGSL
                     GEMHAAKIAHVQDLALKFGAPIVSISDSGGARIQEGIDSLKGYASIFYRNTISSGVIP
                     QISMIAGPCAGGAVYSPGIMDFVVMTDQARMFIT"
     gene            complement(728..1969)
                     /gene="preA"
                     /locus_tag="VN478_02150"
     CDS             complement(728..1969)
                     /gene="preA"
                     /locus_tag="VN478_02150"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006183.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HWQ21513.1"
                     /translation="MYREHDLSYDFLGIHLENPFILSAAPPTDELDMAVDGLNAGWAG
                     VVLKTTSVEGNPVNLKYPMMSSFGTSAHKLWGLGNIDLISQYHIGQICERVTALKTMF
                     PAKMIGASIMGSQKEDWQSLVWQLKKAGVDLIECSFSCPQGSMGEAPGRMLAQSVEAT
                     EKVARWVKEAAGDTPVLIKITPQVTDIVEIARALKRAGVDGVTASNSVPALIGVDVET
                     LEPRPSLFGEGAYSGMTGMAIKPLTLRTIAEIARNVDITISGNGGAYSWSDAVELMAV
                     GASNVQFCTLPMHFGFGTIRDLKSGFADYLDRKGYVSPLEVRGKALKNIKGQEELVTP
                     ESHSDIDLDKCIGCGNCFRACSDGAHRAILWDPEKRQPSVDKEKCPGCGQCMQVCPVN
                     AIRMKEELEGKVHYFEFGGTR"
     gene            complement(1969..3234)
                     /locus_tag="VN478_02155"
     CDS             complement(1969..3234)
                     /locus_tag="VN478_02155"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWQ21514.1"
                     /translation="MEELEKTLSRQQAAIEAERCLYCYDAPCQAKCPAHVPVPEFIQS
                     IRSGNLHGARTLLQTAHPFIETCGRICPEETFCQSVCTRSKIDAPLRIRELHRFVTDS
                     TDPSERMELPEATAGKVAIIGAGPAGLACARELRARGFACDVFEDRRVGGVPAQEVST
                     HRYPREIEEREVRFLTSSFVASVRCERVIDVKHLIGDYDAVFVATGLASESDLNIDGV
                     ELEGVYHARDLLRTMKSGSVSGAGKRAGVIGGGNVAIEVASMLREEDPTRDVTVIYRR
                     GIKELKAFKKEIEEATALGVTYQFLAIPDKVIGNGHVEGLQVAQARLCEEDESGRCRF
                     EPLPGSSFIIPLDTVVVAIGQRIDANVFAGLARSESGLITVGENLETTMRGVYAGGDA
                     VHGAQTVVEAVHDGKAAAEQIAAFVTGGK"
     gene            complement(3455..4429)
                     /locus_tag="VN478_02160"
     CDS             complement(3455..4429)
                     /locus_tag="VN478_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HWQ21515.1"
                     /translation="MDTILKVDHLKKYFAVNTGGPFNRAMASLKAVDDISFDVGRGET
                     VGLVGETGCGKTTVGRTIVGLYKPTAGTIAYCDENLCAMTPEAMRKVRHKLQMIFQDP
                     YASLNPRMTVGGIVEEPMDIFGIGTVQERHERVQELLRTVGLNPEQSNRFPHEFSGGQ
                     RQRIGIARALAMNPEFIIADEPISALDVSIQAQIVNMLEDLQSRLGLTYLFIAHDLAM
                     VKHISNRVVVLYLGKIVEIDESVDIYKHPLHPYTKALLSAVPIPDPRIETKRQRIVLK
                     GDLPSPVNPPSGCHFRTRCPIAQPICAEQEPELRDMGAGHMVACHFAG"
     gene            complement(4431..5501)
                     /locus_tag="VN478_02165"
     CDS             complement(4431..5501)
                     /locus_tag="VN478_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004405396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWQ21516.1"
                     /translation="MDERSVLMRIDGLTTNFHTYAGVVHAVDDMTLTVPKDTVVGIVG
                     ESGSGKSVTMLSVMRLIATPPGRIEGGHVWFDGKALDMPERMEAEGPNAGMVDLLALP
                     AEDMMKVRGRHIGMIFQDPMVSLNPVLTIGLQMTESMVAHGLATEKEAWKRAVDMLAL
                     VGIPRPEERMHDYPHQYSGGMRQRVMIAMALSCSPELVIADEPTTSLDVTVQAQILEL
                     MKELKAKLKSSIIIISHDLSIVAGMADKVVVMYAGRMVEEADVFDLYEKPGHPYTVGL
                     MKSVPRLDAEERERLVPIEGSPPDLLAPPKGCKFAPRCQYAMKVCQEQEPPLVTVGAG
                     HRARCFLHHPAAPNVAGFVKVE"
     gene            complement(5514..6479)
                     /locus_tag="VN478_02170"
     CDS             complement(5514..6479)
                     /locus_tag="VN478_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007935655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWQ21517.1"
                     /translation="MNAQTQWQTAGEKPGLEAGAFAPASTGAGALQEIGGRPTTYWND
                     AVRRLRHNRLAVAGFWIIIFLLVVAIAGPMLSPYSYEQQDLNNTYQSPTLQHPFGTDQ
                     LGRDQLTRVMYGSRISLAVGIVCAALNFIIGVTYGGIAAYFGGKVDEVMMRIVDILYG
                     IPTLIIVILLTVLFKDKGVNPLVNVFIAIGLTYWLPMARIVRGEILSLKEREFALAAK
                     TIGAPNNRILFRHLIPNAMGPIIVTVTLEIPSAIFTEAFLSYIGLGVSAPVASWGSLA
                     SDGTEAIRSFPYLVVFPALAISLTMFAFNFLGDGLRDALDPRLRQ"
     gene            complement(6479..7411)
                     /locus_tag="VN478_02175"
     CDS             complement(6479..7411)
                     /locus_tag="VN478_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018701896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWQ21518.1"
                     /translation="MLKYVIRRLLSMILVVLIVASATFFLMRFLPSGPFSREKALPPA
                     VIKNLEARYHMDQPLWKQYLSYIGNLVRGDLGPSFKYKDRTVNSIIADGFPVSAALGA
                     VAVALAVLIGIPLGIWGALRQNGWQDNFVMFIALLGVSVPNFIMAVLLMLVFALGLHL
                     VPAAGWGSVRQVILPAFTLAGYPIAFIARLVRSSMLEVLRQEYITTARAKGLSERLVI
                     FRHALRNILIPVVTVLGPLIAAVFTGSFVVEKIFAIPGLGRYYVTSINDRDYTTICGV
                     TIFYSALLVFMNFLVDLTYAWLDPRIRYVERKVS"
     gene            complement(7506..9212)
                     /locus_tag="VN478_02180"
     CDS             complement(7506..9212)
                     /locus_tag="VN478_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006715594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HWQ21519.1"
                     /translation="MRKVLSLLLVTALLFGAMAGVRTATAATVEIRWNNGAEPQTIDP
                     ALSTGIPEANTILQVFEGLTRLDKNNNPIPAVAKSWTISTDKKTYMFTLRDSFWTNGT
                     PVTAYDFEYAWKRALQPETAAEYAYQLYYVYGGEAYNKSINVGGKYYKQAVDAKGNPV
                     TKKEGDKDVPVADMKKEIDPSKNVGVRALNAKTLKVYLASPTAYFLSLCAFPTLMPVC
                     KAVVSKNASWTKDDLKAYITNGPFKAISWSHSEKMVFIKNPTYWDKANVKPDKLTYYM
                     IDDESTALAQYQSGALDASDIVPLSELPALVKSGDCKIMPYLGTYYYSFNVTKKPFND
                     VRVRKALTLAINRSAITLNITRAGEIPALGYVPYGIPDATATAEFRTSGTEKFYKDND
                     VATAKALLAAAGYPNGKGFPAFTILYNNSSRHKSIAEAIQQMWKTNLGINCTLKNEEW
                     GVYLDDLTNLNYTTARRGWIGDYVDVNTFMDMWVTGGGNNDTGWSSKQYDADIAKAKV
                     TTDPKARMKLMHDAENILMREFPIAPVYYYTHPILLKSGVKGLVLSTLGFVDWKNATI
                     SG"
     gene            complement(9415..10377)
                     /locus_tag="VN478_02185"
     CDS             complement(9415..10377)
                     /locus_tag="VN478_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HWQ21520.1"
                     /translation="MPFNFNWSIVWWVVGIVVVVVFALRYILIVRQSTAVIVERLGKF
                     SRELKPGLHFLIPILDSVRAVVDLREQAWDYPPQAVITKDNVPTSIDIVLYYYLTDPV
                     KSVYEVRNLNEAILKLTMTSIRNISGSLNLDELLVSRENVNNELTQIVDVATDPWGVK
                     VTRAEIKSVNPPAEILEAMTRQMKAERSKRAAILEAEGFKEAEINKAEGEKQGAILRA
                     EADRQQKILTAEGEAQALITVANARKQAVIAYFQGIHEGGATNDVLALKYMETLEAIS
                     KNPSNKVFMPYESSALMGSLGTIKELFKDVPSATVAATPPAKGK"
     gene            complement(10387..10815)
                     /locus_tag="VN478_02190"
     CDS             complement(10387..10815)
                     /locus_tag="VN478_02190"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HWQ21521.1"
                     /translation="MVGFWITMAVVCAVVDAFLPYYLLVWFSGGAVAALLFSWAGFNT
                     SVQFLVFFLVSFALMALVGRYINSRLLRGGSKMRTNTEEMIGKTASVVMAIEAGGAGR
                     VALKGTTWRAILNDETAVAAVGAKVVVVSIEGVTLTVRKE"
     gene            complement(10953..>11807)
                     /locus_tag="VN478_02195"
     CDS             complement(10953..>11807)
                     /locus_tag="VN478_02195"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HWQ21522.1"
                     /translation="GEKSLSELKQLSADHGTSWFAALPDWIATAKAGKTREKLEAFVD
                     LIVHIGAASESAIAAANLVFASSGLLEQYKDEGDEADFELQSRRENLGEFMRTVDEFD
                     RMSEDRSLGAYLSQVALVQDQDSMKSGVGRGVLLITAHSAKGLEFSEVFVVGAEEGVF
                     PHRRSLDSSADVEEERRLFYVACTRARKELSITYCTQRTSYYGSGFQEPSRFIKEMEL
                     TRQKAAGVLSGERPELIDGDTVMHNIFGAGKVLEVFYDGNDQFVVIDFKKVGVKKLAA
                     QYAQLKKM"
BASE COUNT         2433 a         3562 c         3364 g         2448 t
ORIGIN      
        1 gtgataaaca tgcgcgcctg gtcagtcatg acaacgaagt ccatgatgcc cggagagtac
       61 acggcgccac cggcacaggg accagcgatc atcgatatct gcgggatcac tcccgagctg
      121 atggtgttcc ggtagaagat gcttgcatag cctttcagcg agtcgatgcc ctcctgaatg
      181 cgagctccgc cggagtcgct gatactgacg atgggagctc cgaatttcag ggccaggtcc
      241 tgaacatggg cgatcttggc ggcatgcatc tcaccaagag atccacctgc cactgtgaag
      301 tcctgagagt atacgaagac caggcgagca ccaacgtatc cgtgacccgt gaccactcca
      361 tcgccgggca actcctgctt gtccagcccg aacccggtat tgcggtgact cacgaagacg
      421 ttctcttcct ggaatgatcc tgggtccagc aggagctcga tacgctcacg cgcggtcagc
      481 ttgccggctt cgtgctgctt cttgacacgg ccggggcctc cgcctgcgac gatctgctcc
      541 cttttcgacc gcaaggtcga gaaccgttca tcattgctca tactacctcc tcaggatttc
      601 ggctggcata tgaacgttgc gaatactctt gataagtata gggggttcac ggcagcactc
      661 aaccgacaag acatgcgatc gtcccctgca gcgtctgcca caggggacgc agaagccagt
      721 atcaggttca gcgggttccc ccgaattcga aatagtgcac cttgccctcc agttcttctt
      781 tcatgcggat agcgttgaca gggcacacct gcatacactg cccgcaacca gggcacttct
      841 ctttgtccac ggagggctgt cgcttctctg gatcccagag aatcgccctg tgagccccat
      901 cactgcaggc acggaagcag ttgccacagc cgatgcactt gtcgaggtcg atgtccgagt
      961 gagactcagg cgtgaccagc tcttcctggc ctttgatgtt cttcagcgct ttgcccctta
     1021 cttccagagg cgacacgtat cccttgcggt ccagatagtc ggcaaaaccg ctcttcaggt
     1081 ctctgatggt cccgaaaccg aagtgcatcg gcaacgtgca gaactggaca ttcgaagcgc
     1141 caacagccat cagctcgacg gcatcgctcc aggagtaggc cccaccgttc ccgctgatcg
     1201 tgatgtcgac attccgcgcg atctctgcga tggtacggag cgtgaggggc ttgatcgcca
     1261 tgccggtcat cccggagtag gccccctcgc caaagaggga aggacgtggc tcaagggtct
     1321 ccacgtcgac tccgatcagt gccggtaccg agttgcttgc cgtcacaccg tcgacgcctg
     1381 cccgcttcag cgccctcgcg atctcgacga tgtccgtgac ctgaggcgta atcttgatga
     1441 gtaccggcgt gtcaccggca gcctccttga cccagcgggc aaccttctcg gtcgcctcga
     1501 cactctgggc aagcatccga cccggggcct cccccatact gccctgagga cagctgaaac
     1561 tgcactcgat gaggtcgacc ccggccttct tcagctgcca cacgagcgac tgccagtctt
     1621 ccttctgtga tcccatgatc gaagcgccga tcatcttcgc agggaacatg gtcttcagtg
     1681 ctgtgacgcg ctcgcagatc tggccgatgt gatactgtga aatcaggtcg atgttgccca
     1741 gtccccagag cttgtgcgca gatgtgccaa acgacgacat catcgggtat ttcaggttga
     1801 cgggattgcc ttcgaccgaa gtcgtcttga ggacgactcc ggcccagcct gcgttcagtc
     1861 cgtcgaccgc catgtccaac tcgtccgttg gcggagccgc cgacagaatg aaagggttct
     1921 caaggtgtat gccgaggaag tcgtaggaaa ggtcgtgttc tcgatacatc acttgcctcc
     1981 cgtcacaaac gcagctatct gctccgccgc cgccttgccg tcatgcacgg cctccacgac
     2041 ggtctgggcg ccatgaacgg catcaccacc ggcatagaca ccccgcatag tggtttccag
     2101 gttctcaccg acagtgatca gtccagactc gctgcgagca agaccggcga acacgttcgc
     2161 gtcgatgcgc tgtccgatcg ctacgacgac ggtatcaagc gggatgatga agctcgaccc
     2221 tggcaatggc tcgaatctgc atcggccgga ctcgtcctct tcacagagac gagcctgggc
     2281 aacctgcagt ccctcaacat gcccgttgcc gatcaccttg tccggaatcg caaggaactg
     2341 ataggtcacg ccaagcgccg tggcctcttc gatctccttc ttgaatgcct tcagctcctt
     2401 gatgccacga cggtagatga cggtgacgtc ccgcgtcgga tcctcctcgc gcagcatcga
     2461 ggctacctcg atcgcgacgt tccctccgcc gatgacgcct gcacgcttcc ccgccccgct
     2521 gactgagccg gacttcattg tgcgaagcag atcacgagcg tgatagacgc cttccagctc
     2581 gacgccgtcg atgttcaggt cgctttcgga ggcaagccct gtggcaacga aaaccgcatc
     2641 atagtcacca atcaggtgct tcacatctat cactcgctca cagcgaacac tggcaacaaa
     2701 tgaagatgtc aggaaacgga cctcccgttc ttcgatctcg cgtggatatc gatgggtcga
     2761 cacttcctga gcgggcacac cgcccacccg ccgatcctcg aagacatcac aggcgaagcc
     2821 tctggccctg agttcacggg cacaggccag cccggcagga ccagcaccga tgatagcaac
     2881 cttcccggca gtagcctcag gcaactccat gcgctcggac gggtcggtcg agtccgtcac
     2941 gaaccggtgc agctcccgga tgcgcaacgg cgcatctatc ttcgagcgcg tgcagaccga
     3001 ttggcagaag gtctcttcag gacaaatgcg gccacaggtc tcgatgaacg gatgagccgt
     3061 ctgcagaagg gtcctggcac catgcaggtt gccgctgcgt atcgactgta tgaactcagg
     3121 caccgggaca tgcgcagggc attttgcctg acacggtgca tcgtagcagt agagacagcg
     3181 ttcagcctca atggccgcct gctgccggct cagagtcttc tcaagttcct ccattgttcc
     3241 tcctggttcc catcattgtc agacataccg tggcatcgca ccacggaaga cgaacagagc
     3301 gcgtacccga cggcgcggtg ttctttggaa gctggttccc ttgtgttata cacgaattcg
     3361 ggggttttcc aaccttctct gccctccaga ctgcttcacc aaccaagagc cggcctcacg
     3421 gccggctctt gtgacggaaa cgatgtggat gcactcagcc cgcgaagtgg caggcgacca
     3481 tgtgacccgc acccatgtct cgcagttcag gttcctgctc ggcgcagatg ggctgggcga
     3541 tggggcagcg ggttcggaag tgacacccac tcggcggatt cacagggctg gggaggtccc
     3601 ccttcaggac gatgcgctga cgctttgtct caatgcgcgg gtcgggaata ggaacggccg
     3661 agagcagtgc cttggtatac gggtgcagtg gatgcttgta gatgtccacg ctctcgtcga
     3721 tctcgacgat cttgccaaga tacaggacga ccacgcggtt gctgatgtgc ttgaccatgg
     3781 caaggtcgtg ggcgatgaag aggtaggtca gcccaagacg ggattgcagg tcctcgagca
     3841 tgttgacgat ctgtgcctgg atacttacat ccagtgcaga gataggctcg tccgcgatga
     3901 tgaactcagg gttcatggcg agagcgcggg cgataccgat acgctgacgc tgcccacccg
     3961 aaaactcgtg cggaaaacgg ttggactgtt cggggttgag ccctaccgta cgcaggagct
     4021 cctgaacacg ttcgtgccgc tcctgcactg tcccgatgcc gaagatgtcc atgggttctt
     4081 ccacgatgcc cccgaccgtc atgcgaggat tgagggaagc ataaggatcc tggaagatca
     4141 tctgcaactt gtgacgcacc ttgcgcatgg cctccggggt catggcacac aggttctcgt
     4201 cgcagtacgc gatcgtgccg gctgtgggct tgtagaggcc gacgatcgtc ctgccaacgg
     4261 tggtcttgcc acaacctgtc tcaccgacca gacccacggt ctcgccgcga ccgacgtcga
     4321 aggaaatgtc atccacagct ttcaggctcg ccatagcacg gttgaagggt ccacccgtgt
     4381 tgacggcaaa gtacttcttg aggtgatcga ctttcagaat ggtatccata tcactccacc
     4441 ttcacgaagc cggccacatt gggggcagct ggatggtgca ggaagcacct tgcgcggtgc
     4501 cctgctccta cggtcacgag cggcggctcc tgctcctggc agaccttcat ggcgtactgg
     4561 cagcggggag cgaatttgca gcccttgggc ggtgccagca ggtctggagg actcccttcg
     4621 atgggcacga ggcgttcccg ttcctcggca tcaagtcgcg gaaccgattt cataaggccc
     4681 accgtatatg ggtgcccggg cttctcatag agatcgaaga catcggcttc ttcgaccatg
     4741 cggccggcat acatgacaac aaccttgtcg gccatgcccg ccacgatcga gaggtcgtgg
     4801 ctgataatga taatgctcga cttgagcttg gccttgagtt ccttcatgag ctccaggatc
     4861 tgcgcctgga ctgtcacgtc cagagaggtc gtgggctcat ctgcgatgac gagttcgggg
     4921 ctgcatgaaa gcgccatggc aatcatgaca cgctgccgca tgccgccgga atactggtgg
     4981 gggtagtcgt gcatccgttc ctcgggcctc ggaataccga cgagagcgag catgtctact
     5041 gctctcttcc aagcctcctt ctctgtggcc agcccgtgcg ccaccataga ctctgtcatc
     5101 tgaaggccga tcgtcaggac cgggttgagc gagaccatgg ggtcctggaa gatcatgcct
     5161 atgtggcgtc ctcgcacctt catcatgtct tcggcaggga gagcgagaag gtccaccatg
     5221 cctgcattcg gaccttccgc ttccattcgc tccggcatgt ccaaagcctt gccatcaaac
     5281 cagacatgtc caccctcgat acgtcccgga ggagtcgcaa taagacgcat gacggacagc
     5341 atcgtcacgg acttgccgga cccggactcg cctacgatgc cgaccacagt gtccttgggc
     5401 acagtgagtg tcatgtcatc caccgcatgg actacgcccg cataggtgtg gaagttggtg
     5461 gtcagtccat caatgcgcat aagaacgctg cgttcatcca ttgtgcacct tccctactgc
     5521 ctgagacgcg gatccagcgc atcgcgcagg ccgtcgccca ggaagttgaa tgcaaacatc
     5581 gtgagactga tggccagcgc cgggaacacg accaggtacg ggaacgaccg gatggcttca
     5641 gtgccatcag acgccagtga gccccagctt gcgacaggag cgctgacacc cagcccgatg
     5701 tagctgagga acgcctcggt gaagatggca gaagggatct cgagcgtgac cgtcacgatg
     5761 ataggaccca tagcgttcgg gatgaggtgc cggaagagaa tgcgattgtt cggtgcgccg
     5821 atggtcttgg cggccagcgc gaactcccgc tctttgagcg aaaggatctc gccgcgcacg
     5881 atgcgcgcca tgggcagcca gtaggtaagc ccgatggcga tgaacacgtt gaccagcggg
     5941 ttcacccctt tgtccttgaa gagaacggtc aggagaatga cgatgatcag cgtcgggatc
     6001 ccgtacagga tgtccacgat acgcatcatg acctcgtcga ccttgcctcc gaagtaagcg
     6061 gcgatcccgc cataggttac gccgataatg aagttcagag ctgcgcagac gatacccacg
     6121 gcaagcgaaa tgcgggaacc gtacatgaca cgcgtcaact ggtcacggcc gagctgatcc
     6181 gtgccaaagg gatgctgaag tgtcggcgac tgatacgtat tgttgaggtc ctgctgttcg
     6241 tagctgtagg gcgacaacat gggcccggcg atcgcaacaa cgaggaggaa gatgatgatc
     6301 cagaaccctg caaccgccag cctgttgtga cgaaggcgac gaacagcgtc gttccagtat
     6361 gtcgtgggtc ggccaccgat ctcctgcaga gctcctgccc ccgtggatgc aggagcgaag
     6421 gctcctgcct caagcccggg tttttcgccc gctgtctgcc actgcgtttg agcattcatc
     6481 aggacacctt ccgttcaaca taccggatac gcgggtcgag ccacgcatac gtgagatcga
     6541 ccaggaagtt catgaagacc agaagcgcgc tgtagaagat ggtaacgccg catatggtcg
     6601 tgtagtcacg gtcattgatg ctggtaacgt agtagcggcc caggccgggg atcgcgaaga
     6661 tcttctccac gacgaagctg cctgtgaaga ctgccgcaat gagcgggccc agaacggtca
     6721 cgaccgggat gaggatattg cgcagggcgt gccggaagat gacgagacgt tctgaaaggc
     6781 ccttggcccg tgcggtggtg atgtactcct gccgcaggac ctcaagcatg gaggagcgga
     6841 caagccttgc gatgaaagcg atgggatacc ccgccagagt gaacgcgggc aagatcacct
     6901 gccgtacgct gccccagccg gctgccggca cgagatgaag cccaagagcg aagacaagca
     6961 tcagtagcac agccatgatg aagttgggga ccgagacccc cagcaaggct atgaacatga
     7021 cgaagttgtc ctgccacccg ttctgccgca gtgcacccca gatacccagg gggatcccaa
     7081 tgagcacggc gagagcgacc gcgaccgcgc ccaaggctgc agaaacgggg aatccatcgg
     7141 caatgatgct gttcacggta cgatccttgt acttgaagct gggaccgagg tctccgcgga
     7201 ccaagttgcc aatgtagctg agatactgtt tccaaagtgg ctgatccatg tggtaccgcg
     7261 cctcaaggtt cttgatgacc gcaggaggaa gcgccttctc acgcgagaac ggccccgaag
     7321 gcaaaaaccg cataaggaag aaagtggccg aggcgacgat caggaccacc aggatcatcg
     7381 acagaagacg acgaatgacg tatttcagca tagctcctcc accagacatt caaggtggcc
     7441 cacaggtcac tgtgggccac cttgacagaa ttcaagctag gacagggata cggccatccg
     7501 ctccgctatc cggagatggt agcattcttc cagtcgacga agccgagagt cgacaacacc
     7561 aggcccttga ccccggactt cagaagaatc gggtgagtgt agtagtagac cggcgcaatg
     7621 gggaactccc tcatcaggat gttctcggcg tcgtgcatca gcttcatgcg agcctttgga
     7681 tctgtcgtaa ccttggcctt tgcgatatcg gcatcgtact gcttgctgct ccagccggta
     7741 tcgttgttgc caccgccggt gacccacata tccatgaacg tgttcacgtc gacgtagtcg
     7801 ccaatccacc ctctgcgcgc agtcgtgtag ttcaggttgg tcagatcgtc caggtagaca
     7861 ccccattcct cgttcttcag ggtgcagttg atacccaggt tggtcttcca catctgctgg
     7921 atcgcctcgg cgatggattt gtggcggctc gagttgttgt agaggatggt gaaggccggg
     7981 aaacccttcc cattgggata gccagccgct gcaagcagcg ccttggcagt cgcaacatcg
     8041 ttgtccttgt agaacttctc ggtaccagac gtacggaact cagcagtcgc ggtggcgtcc
     8101 gggataccgt atgggacata gcccagggcc gggatttccc ctgcgcgagt gatgttgagc
     8161 gtgatggcgc tgcggttgat tgccagcgtc agcgccttgc ggacgcgaac gtcattgaac
     8221 ggcttcttcg tgacgttgaa gctgtaatag taggtgccga gatacggcat gatcttgcag
     8281 tcgccggatt tcaccagcgc cggcagctcg gaaagcggca caatgtcaga ggcgtcgaga
     8341 gccccactct gatactgagc gagggcagtc gactcatcgt cgatcatgta gtaggtcagc
     8401 ttgtcgggct tgacgtttgc cttgtcccag tatgtcgggt tcttgatgaa caccatcttc
     8461 tcactgtgtg accatgagat ggccttgaag ggtccgttgg tgatatacgc cttcagatca
     8521 tccttcgtcc agctcgcgtt cttggagacg acagccttgc agacgggcat gagcgtcggg
     8581 aacgcacaca gactgagaaa gtaggctgta ggtgaagcga gatagacctt gagcgtcttg
     8641 gcattcagag cacggacgcc gacgttcttg ctgggatcga tctccttctt catgtcagcg
     8701 actgggacgt ccttgtctcc ctctttcttg gtgaccgggt tgcccttggc gtcgacagcc
     8761 tgcttgtagt atttgccacc gacattgatg gacttgttgt aggcctcgcc accgtagaca
     8821 tagtacagct ggtacgcgta ctctgctgca gtctcgggct ggagggcgcg tttccaggca
     8881 tactcgaaat catatgcagt aacgggcgtg ccgtttgtcc agaaggagtc acggagggtg
     8941 aacatgtagg tcttcttgtc cgtcgagatg gtccagctct tggcgacggc cggtatcggg
     9001 ttgttgttct tgtccagtcg tgtcaagcct tcaaagacct gaagaatcgt attggcctcg
     9061 ggtattccgg tggacagcgc cgggtcgatg gtctgcggtt cggctccgtt gttccatctg
     9121 atttcgacgg ttgcggcggt cgcagttcgc acgcctgcca tggcgccgaa gagcaatgca
     9181 gtgacgagta gcagcgatag gaccttcctc atctgattcc tcccctttcc agagaatgcg
     9241 tcgcactggg cgactttccg tatgctagcc cgtcggccaa aaaaagcaaa tgaccttcgg
     9301 tcgccggtcc cctcccgccg gcaaccctcg catatcgccg tggacaacga accagccgga
     9361 cccctgagcg ggggtccggc tggataccat ggtatgttcg aaaccgcgca gcgactactt
     9421 gcccttcgcg ggcggagtcg cagcgactgt cgctgaggga acgtccttga agagctcctt
     9481 gatcgtacca aggctcccca tgagtgcgct gctctcgtat ggcatgaaca ccttgttgga
     9541 cgggttcttg ctgatagcct caagagtctc catgtacttc agcgcgagca cgtcattggt
     9601 ggcgccgccc tcgtggatgc cctggaaata tgcgataacc gcttgcttgc gagcgttggc
     9661 cacggtgatc aatgcctgcg cttcgccctc ggcggtcagg atcttctgct gccggtcggc
     9721 ctcagcacga aggatcgcgc cctgcttctc tccttcggcc ttgttgatct cagcttcctt
     9781 gaacccctcg gcttcgagaa tggcagcacg tttgctgcgc tcggccttca tctgccgtgt
     9841 catggcctcg aggatctcag ccggcgggtt caccgacttg atttccgcac gcgtgacctt
     9901 gacgccccac gggtcggtcg ccacatcgac gatctgcgtc agctcgttgt tgacgttctc
     9961 gcgagagacg agcaactcat ccaggttcag gctgcccgaa atgttgcgaa tggacgtcat
    10021 cgtgagtttg agaatagcct cattgagatt gcgcacctcg taaaccgatt tcacggggtc
    10081 cgtgagatag tagtagagaa cgatgtcgat gctggttgga acgttatcct ttgtgatgac
    10141 ggcctgaggc ggatagtccc atgcctgctc gcgcaagtcg acgactgccc tcacgctgtc
    10201 gaggataggg atgaggaagt gcaggccggg cttcagctcg cggctgaact ttcccaggcg
    10261 ctcgacgatg actgcggtac tctggcggac aatcaagatg taccgcagcg cgaagaccac
    10321 cactacgacg atcccgacga cccaccagac aatggaccag ttgaagttga atggcatgag
    10381 tacctcctat tcctttctca cggtcaaagt gacaccctcg atcgacacga cgacaacctt
    10441 cgcacctacg gcggcgacgg ctgtctcatc gttgaggatg gctctccacg tcgtgccttt
    10501 gagcgctacg cgtcctgcgc cgccagcttc aatagccata accacgctgg ccgtcttgcc
    10561 tatcatctct tcagtattcg ttcgcatctt ggaaccaccc ctcaacaaac gtgagttaat
    10621 gtagcgccca acgagtgcca tgagcgcaaa cgataccagg aagaacacga ggaactgcac
    10681 tgacgtgttg aacccggccc aggagaacaa gagcgccgcg acagccccgc ccgagaacca
    10741 gacaagcagg tagtagggaa gaaaagcgtc caccactgcg cagacgaccg ccatcgttat
    10801 ccagaaaccc accatgcgca cctcctcgaa accgaacagc cctgattgca ctccaatact
    10861 ttcatcttac tatcgaatgc agacttcacc atcatacaca gaccgcccaa taagaaaagc
    10921 acgagtctgt gcggagacgt acacccgccg cgtcacatct tcttcagctg agcatactgc
    10981 gctgcgagct tcttgacgcc caccttcttg aagtcaatca cgacgaactg atcgttcccg
    11041 tcatagaaca cctcaaggac cttcccggcc ccgaaaatgt tgtgcatgac agtgtcaccg
    11101 tcgatcagct ccggcctctc accggacagc actccggcag ccttctgcct ggtcagttcc
    11161 atctccttga tgaaccgcga tggttcctgg aatcccgacc cgtagtagct ggtacgctgg
    11221 gtgcagtatg ttatcgacag ctccttccgc gcccgtgtgc atgctacgta gaagagtctt
    11281 cgctcttctt cgacgtcggc gctgctgtcc agcgagcgac ggtgagggaa gactccttcc
    11341 tctgcaccga ccacgaacac ctcactgaac tccaaaccct tggcggaatg cgccgttatc
    11401 agcaggacgc ctctgcccac tcccgatttc atactgtcct ggtcctgaac cagcgcaacc
    11461 tgcgacaggt aggccccaag cgatctgtcc tcgctcatgc ggtcgaactc gtcgacagtg
    11521 cgcatgaact ccccgaggtt ctcgcgtctc gactgcaatt cgaagtccgc ttcgtctccc
    11581 tcgtccttgt actgttcgag aagtcccgac gacgcaaaca ccaggttggc tgccgctatg
    11641 gctgactcgc tggctgcgcc gatgtgaacg atgagatcga cgaacgcctc aagcttctcc
    11701 cgtgtcttgc ccgccttcgc cgtggctatc cagtctggaa gcgccgcaaa ccatgacgtg
    11761 ccatggtcgg cagacagctg cttcagttcg gatagactct tctcacc
//
LOCUS       DATMZD010000103         2210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_42975,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000103 DATMZD010000000
VERSION     DATMZD010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42975"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1675)
                     /locus_tag="VN478_02200"
     CDS             complement(<1..1675)
                     /locus_tag="VN478_02200"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HWQ21523.1"
                     /translation="MNWANLTERAQKAVWMAQESASSLGNDFLDTDHLLIGLAKLEEG
                     IAFEAMVASGIDLHKLVDYVSDQVRGVKHNNFLGRNDEIILTPRAKKVLDLAEREAIN
                     LGDGYISTEHILLAIVHEGGGVGVRNLEDEGVDISKLVNTVLSLRGTEHAPESPRPGH
                     QPRQRTKTPTLDQYSRDLTEMARQGRLDPVIGRDKETERLLQILLRRTKNNPVLIGDP
                     GVGKTAIVEGLARKIAAGEVPELLKNKRVISLDIPSLVAGTKYRGEFEDRMKKFLAEM
                     KTAGNTVILFIDELHNVVGAGSAEGSSIDAANILKPALSRGELQTIGATTLGEYHKYI
                     EKDPALERRFQPIVVSESTVPESVAILKGLRDKYEAHHQVTITDAAIDAAVRLSVKYI
                     TSRFLPDKAVDLMDEASAKVRLASAVPSDEYRQLQDELDKIEREKDDAVRNQEFEKAA
                     GLRDQERDLHDRIETMKGSSLVASAAREAVVSPDDIRAVVALWTNIPAERLAEDERAK
                     LLHLEDALRTRVVGQDGAVALMAEALRRSRAGLKHPTKPIGSFLFLGPTG"
     gene            1881..>2210
                     /locus_tag="VN478_02205"
     CDS             1881..>2210
                     /locus_tag="VN478_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21524.1"
                     /translation="MDDKADAESTPRDWFAELTARVPNFVGYQVRDQRRIADKTVRDT
                     MVFKLDDLREILSDVIEKANDAETSIVDDISKILRTMNELRDKVEFLGYGDTTFFTDE
                     QLDPGFLS"
BASE COUNT          418 a          675 c          615 g          502 t
ORIGIN      
        1 cacccgtcgg gcccaggaac agaaacgacc caatcggctt tgtagggtgc ttgagccctg
       61 cacgcgaacg tctcagggcc tccgccatga gcgctacagc cccatcctgt cccacgactc
      121 tggttcgaag ggcatcttcg aggtgaagca gtttggctcg ttcgtcctca gccagacgct
      181 cggccgggat attcgtccag agggcaacga cagcgcggat atcgtccgga ctcacgaccg
      241 cctcccgcgc ggccgatgcg accagcgaac tgcctttcat cgtttcgatc ctgtcgtgaa
      301 ggtcccgttc ctggtctcgc agaccagccg ccttctcgaa ctcctggttg cgaacggcat
      361 cgtccttctc tcgctcgatc ttgtcgagtt cgtcttgcag ctggcggtac tcgtcgctcg
      421 gaaccgcaga cgcgagcctt accttggcgc tggcttcgtc catgagatca actgccttgt
      481 ccggcaggaa ccggctcgtt atgtacttca ctgaaagcct gacggcagcg tcgatggccg
      541 cgtccgtaat agtcacctgg tggtgggcct cgtacttgtc gcgcagtccc ttcaggatcg
      601 caaccgattc cggaaccgtc gactcgctga cgacgatagg ctggaaccgg cgctcgagtg
      661 cgggatcctt ctcgatgtac ttatggtact ctccaagcgt cgtcgctccg atcgtctgca
      721 gttcgcccct cgaaagcgcc ggcttgagaa tgttggcagc gtcgatggaa ctcccttctg
      781 cggatcctgc gccgacgacg ttgtgaagct cgtcgatgaa caggatgacc gtgttgccgg
      841 ctgtcttcat ctccgctaga aacttcttca ttcgatcttc gaactcgcca cggtacttgg
      901 tccctgccac aaggctcggg atgtccagcg agatcacacg cttgttcttg agcagctccg
      961 gcacttcgcc ggctgcgatc ttccgtgcga gcccctcgac aatagccgtc ttgccaacgc
     1021 cggggtcgcc gatgagaacc ggattgttct tggtccgtcg cagcaatatc tgaagcagcc
     1081 gttcggtctc cttgtcgcga ccgatgacag ggtcaagtct cccctggcga gccatttccg
     1141 tcagatcgcg actgtactgg tcgagtgtcg gcgtcttcgt gcgctgccgt ggctggtgac
     1201 cggggcgagg cgactccggc gcgtgctcgg tcccacgcaa tgagaggact gtgttcacga
     1261 gcttggagat atcgacacct tcatcctcca gattccgaac gcccacacca ccaccctcgt
     1321 ggacgatcgc gagcaggatg tgctcggtgg aaatatagcc gtcaccaagg ttgatcgcct
     1381 cgcgctcggc caggtccaga accttcttgg ccctgggcgt gaggatgatc tcgtcgtttc
     1441 tgccgaggaa gttgttgtgc ttgacacctc gtacctgatc cgagacgtag tccaccagtt
     1501 tgtgaaggtc gatgccactg gcgaccatcg cctcaaacgc tattccctct tccagcttgg
     1561 caagtccgat caggaggtga tccgtgtcaa ggaagtcgtt gcccagagag ctggccgact
     1621 cctgtgccat ccacacagcc ttttgagcac gttctgtcaa gtttgcccag ttcatagaat
     1681 gatgcctcca ttcccggcat tgcttctgac acgaatgggg accggatccc cacaaatctg
     1741 tttctatgat agtttaccgc acccatgcct ttcgtcaaac cactgactgt ggcgagcgcg
     1801 ggcttctatc gtggtttgac ttgttctgtc accacatgta gaattcgttt agtagactct
     1861 tggtggcaaa gggggatgct atggatgaca aggcggatgc agaaagcaca ccgcgtgact
     1921 ggttcgcaga actgacagct cgcgtcccga atttcgtggg ctatcaagtc agggaccagc
     1981 gccgcattgc cgacaagaca gtgcgagaca ccatggtatt caagctggat gacttgaggg
     2041 agatcctgtc cgacgtcatc gagaaggcaa acgacgccga aacgtccatt gtcgacgaca
     2101 tctccaagat cctcagaacg atgaacgagc tgcgtgacaa ggtggagttc ctcggctacg
     2161 gcgataccac gttctttacc gacgaacagc ttgaccccgg tttcctgtcc
//
LOCUS       DATMZD010000104         2509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_53372,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000104 DATMZD010000000
VERSION     DATMZD010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53372"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..940)
                     /gene="ruvB"
                     /locus_tag="VN478_02210"
     CDS             complement(<1..940)
                     /gene="ruvB"
                     /locus_tag="VN478_02210"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF000868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HWQ21525.1"
                     /translation="MAAGEEKRTRRKAPVAQAGPSAAKVEADTVGSMVRPRCLEDFIG
                     QAQLRRNLTVFIKAARSRNEPLDHCLLYGPPGLGKTTLSQIIAAELGVRFKSTSGPAL
                     TRAGDLASILASLRERDVLFIDEIHRLPPAVEESLYSAMEDYHLPIIYGKGPSAKIMT
                     IPVKPFTLIGATTRFGMLSAPLRDRFGIIMRMDPYDEDELAQIIANAARRLDTDVEPA
                     AAMRIAGRSRGIPRVALRNLKRIRDYLAYAEASVVDVRLVDEAFSRLNIDKYGLLDID
                     RRLLAAIDDKFDGGPVGLDSLASAISEDPDTVSVVIE"
     gene            complement(944..1537)
                     /gene="ruvA"
                     /locus_tag="VN478_02215"
     CDS             complement(944..1537)
                     /gene="ruvA"
                     /locus_tag="VN478_02215"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HWQ21526.1"
                     /translation="MIALIRGTVYRRLDSAIIVLVGDVGYEVRVVDPATFEVGEEVEL
                     RTYHVVKEDRQELYGFVESLDYEVFADLIEHVPGVGAKTALGLLRAAPASRLIEAVRE
                     QNPGLLTSAPGVGRKNAERILIELRPIVNRKYSELVSIEGPAGTEPALLAEVEMALRS
                     LGFSQREVSGALRELGATKDRSAEELVSDALQLLARK"
     gene            complement(1521..2036)
                     /gene="ruvC"
                     /locus_tag="VN478_02220"
     CDS             complement(1521..2036)
                     /gene="ruvC"
                     /locus_tag="VN478_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007431677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HWQ21527.1"
                     /translation="MLTLGIDPGLARIGYGVVSSDGDTVALLDYGCLETHAGTPYADR
                     LKYIYDGVRRLVRRYKPDAVALESLFFFRNARTVMKVSEARGVIVLAVGTCHVPAFEY
                     TPQQVKQAVSSYGMETKRNVEIAVRQILGVEERITPDDTADAIAIALCHTFTGAYREA
                     VLAEETDDCSD"
     gene            complement(2044..>2509)
                     /locus_tag="VN478_02225"
     CDS             complement(2044..>2509)
                     /locus_tag="VN478_02225"
                     /inference="COORDINATES: protein motif:HMM:NF013841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HWQ21528.1"
                     /translation="IIQASTDNKNRTTSEIRRILSQHGGALGENGSVSWGFERKGSLE
                     IESSDKGYDDLFMVVVDCGGEDLKQDDETTTVITGPEDVYKVRQALEAAGVVVKSASL
                     TMIPKTLVAVAIEDARKIARLEEALDEHDDVSDYFSNYEMSDDVIKQLEQES"
BASE COUNT          492 a          788 c          707 g          522 t
ORIGIN      
        1 gctcaatgac aactgacacg gtgtccggat cttcgctgat cgcggaagcc aagctgtcaa
       61 gccctacagg accgccatcg aatttgtcgt caatggcagc gaggagccgt cgatcgatgt
      121 ccagcaatcc atacttgtcg atgttgagac gcgagaacgc ctcgtcgacc agtctgacat
      181 cgacgactga agcctccgcg tacgccaggt agtctctgat gcgcttcagg ttgcgaagag
      241 ccacgcgggg aataccgcgc gagcgacccg cgatcctcat ggcagcggct ggttcgacgt
      301 ctgtgtcgag cctgcgggcg gcgttggcga tgatctgcgc cagttcgtcc tcatcgtacg
      361 gatccatccg catgatgata ccgaacctgt cgcgcagcgg agctgacagc atgccaaacc
      421 gtgttgtcgc tccaatgaga gtgaacggct tcaccggtat cgtcatgatc ttggcgcttg
      481 gccctttccc atagatgatg ggcaaatgat agtcctccat ggccgagtac agggactctt
      541 ccaccgcggg gggcagacgg tggatctcgt caatgaacag gacgtcccgc tccctgagcg
      601 acgccaggat gctggccagg tcccctgccc gtgtcagcgc aggcccactg gtgctcttga
      661 accgaacgcc gagctctgcg gcaatgatct gcgagagcgt tgtcttgcca agacccgggg
      721 gaccgtagag cagacagtgg tccagcggct cgttgcggct tcgcgcagcc ttgatgaaga
      781 ccgtcaagtt gcgtcgcaat tgggcctgcc cgatgaagtc ctcaagacac cgtggccgca
      841 ccattgagcc gactgtgtca gcctcgacct tcgccgctga cggaccagcc tgggcgacag
      901 gtgccttgcg acgcgtgcgt ttctcttcgc cggccgccat ggcctacttc ctcgccagaa
      961 gctgcaatgc atcgctcacg agttcctcgg ctgaccgatc cttcgtggca ccgagctccc
     1021 gaagagcccc actgacctcg cgttgtgaga atccaagaga ccgaagcgcc atctccacct
     1081 ctgcgagtag agccggttcg gtccctgcag ggccttcaat ggacacaagc tccgagtact
     1141 tgcggttcac gatgggccgc agctcgataa ggatgcgttc tgcgttcttc ctgcccacgc
     1201 ctggagccga agtgagcagt ccagggttct gttctctcac cgcttcgatg aggcgagacg
     1261 caggcgccgc acggagaagc cccagcgcgg tcttggcccc gacgcccgga acatgctcga
     1321 tcaagtcggc gaacacctcg tagtcgagtg attcgacaaa accatagagc tcctgtcggt
     1381 cttccttgac gacatggtac gtccgcaact cgacctcttc gccaacctca aacgtcgcgg
     1441 ggtcgacaac tctgacctca tacccgacgt ctcccacgag aacgatgatg gcggaatcca
     1501 gcctgcgata tacggttccc ctaatcagag caatcatctg tctcctccgc caagaccgcc
     1561 tcgcgatagg ctcccgtgaa tgtatggcag agagcgatgg ctatcgcatc tgccgtgtcg
     1621 tccggtgtga tacgctcctc gacgccgaga atctgacgca ccgcaatctc gacattcctc
     1681 ttggtctcca tgccgtagga cgacactgcc tgcttgactt gctgaggcgt gtactcgaat
     1741 gcggggacgt gacacgtgcc gactgcaaga acgatgactc cacgagcctc gctgaccttc
     1801 atgaccgtcc tcgcgttgcg aaagaagaac agcgactcga gggccactgc atccggcttg
     1861 tacctgcgga cgagtcggcg aacaccgtcg tagatgtact tgagcctgtc tgcatatggc
     1921 gtgcccgcat gcgtctccag gcagccatag tcaagaaggg ctacggtgtc gccgtctgat
     1981 gagacgacac cgtagccgat gcgtgccagt ccgggatcga ttcccagagt aagcattgac
     2041 tatttagctt tcctgctcca gctgcttgat gacgtcgtcg ctcatctcat agttggagaa
     2101 gtagtcgctg acatcatcgt gttcgtccag cgcttcctcg agccgcgcga tcttccgggc
     2161 atcctcgatg gctacagcga cgagggtctt gggaatcatg gtgagagatg cagacttcac
     2221 gacaacgcct gcagcttcca gagcctgacg caccttgtag acgtcctccg gccccgtgat
     2281 gacggtggtt gtctcatcat cctgcttgag gtcttctcca ccgcagtcga caacaaccat
     2341 gaacagatcg tcgtacccct tgtcagacga ctcgatttcc agcgaaccct tccgctcgaa
     2401 gccccaactg acggatccgt tctcacccag cgcgccaccg tgctgtgaaa ggattctcct
     2461 gatttcggac gtcgtacggt tcttgttgtc ggtcgaagcc tggatgatg
//
LOCUS       DATMZD010000105         3863 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_93671,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000105 DATMZD010000000
VERSION     DATMZD010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3863
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93671"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="VN478_02230"
     CDS             <1..487
                     /locus_tag="VN478_02230"
                     /inference="COORDINATES: protein motif:HMM:NF013380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HWQ21529.1"
                     /translation="GSTAVQGRSGSKDWDVIAAAKAKATIPIIANGGVKKAEDAKRFL
                     DYTGADYVMIGRAALGNPWVFEQINDVLDGRSPREPTLEERFRVMQEHVDREVVLKGE
                     IIGVKEMRKHLAFYFRGLPEATTYRGRINQVKEADQLKKLLNEYRVLCTRDGEEVCHD
                     A"
     gene            477..1016
                     /gene="greA"
                     /locus_tag="VN478_02235"
     CDS             477..1016
                     /gene="greA"
                     /locus_tag="VN478_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HWQ21530.1"
                     /translation="MMHDEEERFDEEIEITNVREDEQEKLYISPSQHNKMLEELDRLK
                     TKERLRIAERIKEAKGFGDLSENAEYEEAKKEQAFIEGTIMDLERQLEHSVVVEPNDT
                     GTGEVHMGCKVQVLDVETAKTRWFTIVGNLEADPMEGRISIDSPVGKALVGKHEKETV
                     AVRIPKGRVSYKILTIERV"
     gene            1019..2491
                     /gene="lysS"
                     /locus_tag="VN478_02240"
     CDS             1019..2491
                     /gene="lysS"
                     /locus_tag="VN478_02240"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HWQ21531.1"
                     /translation="MLELNEVEQSRRDKIGELRQQGIDPFGHGFGGSLSTAYIAEHFD
                     ELGETTVRARGRVMAVRGHGKACFLVLADMAGRIQLYLRSDDLKEPAYDWFKKYVDSG
                     DIIGVEGTLFVTKTGEKTIAVERLWVLSKAIRTLPEKFHGLTDVEIRYRQRYVDLIVN
                     PEVRDTFEKRSRMVSRIRTYLTERGYLEVETPMLQPIAGGATARPFVTHHNALDQDLF
                     LRIAPELYLKRLLVGGFEKVFEINRSFRNEGIDTVHNPEFTMMELYEAYSDLDGMMTL
                     TEDLILDLVKTITGVSEVHFGERLINFRGPFARTTFDDAMRRYAGVGLEELRDDGLAR
                     RKISESGIHMDKSFEYANVVNELFDKMVEPNFVDPTFVHDYPVEISPLAKRMAEQPQR
                     VHRFELFAGGMELANAFTELNDPIDQEERFVQQAAAKDRGDDESQSMDFDYVRALQYG
                     MPPAGGLGIGIDRLVMLLLGVESIREVILFPQLKRRESQD"
     gene            2517..>3863
                     /locus_tag="VN478_02245"
     CDS             2517..>3863
                     /locus_tag="VN478_02245"
                     /inference="COORDINATES: protein motif:HMM:NF024872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HWQ21532.1"
                     /translation="MAKPQVQYRCSECGHVTVSKLGKCPECGSWGSFVEDIPQVEAVA
                     RPPSRVLTSGILPAEIDLEVAVENVQPERRMVTGIGPWDEVLGGGLVVGSAVLVGGEP
                     GIGKSTLVLQAATALGCHGKVLYVSGEESRSQILGRVRRLGLDPTNLLLATTNSLADA
                     LAMAERARPVALVMDSLQSFSTEEDFFGSGTPAQLKTTAAAIAGFSKKSGVAALIIGH
                     VTKDGAIAGPKYVEHLVDAVLYMEGDRFGAYRLLRSGKNRYGKSGETGFFEMTSSGLV
                     AISDPSSAFVTDVHNLQPGSVVVPAAQGSFAVLVEVQSLAATSYLPSPRRIATGLDYG
                     RLMIALAVIERRAGTSAGGRDVYVNISGDFSVEDRGIDLGVTLSLYSAIRNVAMREGV
                     MPIAEVGLAGDLRPVFNLHRRIELGARMGFKEFIVSDKCRDDLADLRGLTFHRKRYV"
BASE COUNT          851 a         1005 c         1242 g          765 t
ORIGIN      
        1 cggcagtact gccgtgcagg ggcgttcagg ttcgaaggat tgggacgtca tcgcagccgc
       61 aaaggcgaag gcgaccattc cgatcatcgc gaacggtggt gtcaagaagg ccgaggacgc
      121 gaagaggttt ctcgactaca ctggagctga ctatgttatg ataggacggg cagctctcgg
      181 aaacccgtgg gtctttgaac agatcaatga cgtcctggat ggcaggagtc ctcgtgaacc
      241 cacacttgaa gaacggttca gggtgatgca ggagcatgtc gaccgcgagg tcgttctcaa
      301 gggagagatc atcggagtca aggagatgcg caagcacctt gctttctact tcagggggct
      361 tcccgaggcg acgacatatc gagggcgtat caaccaggtg aaggaagcag accagctgaa
      421 gaagctgctg aatgagtacc gtgtgctgtg cacgcgtgac ggcgaggagg tttgccatga
      481 tgcatgacga agaagagcgt tttgacgaag aaatcgagat cacaaacgtg cgggaagacg
      541 agcaggagaa gctttacatt agcccatcgc agcacaacaa gatgcttgaa gagctcgaca
      601 ggctgaagac caaggaacgc ctgcgcatcg cagagcgcat caaggaagca aaaggatttg
      661 gcgacctctc ggagaacgcc gagtatgagg aggccaagaa ggagcaggcg ttcatcgagg
      721 ggactatcat ggacctcgaa cgccagcttg agcactctgt ggttgttgag ccaaatgaca
      781 ccggcacggg cgaggttcac atgggttgca aggttcaggt tctcgacgta gagacggcca
      841 agacgcgctg gttcaccatc gtcggaaatc tcgaagcgga tccgatggag ggacgcatct
      901 ccattgatag tccagtgggc aaggcgctcg tgggaaaaca cgagaaggag accgtggcag
      961 tgcgcatccc caagggtcgc gtctcgtaca agatcctgac tatcgagagg gtatagacgt
     1021 gcttgagctg aacgaggttg agcagagccg ccgggacaag ataggggagc tgcgtcagca
     1081 ggggatcgac ccgtttggcc atggctttgg cgggtccctg tcgaccgcct acatcgcgga
     1141 acacttcgat gaactggggg aaaccacggt tcgcgccaga ggacgtgtca tggctgtccg
     1201 tggtcatggg aaggcgtgtt tcctcgtact ggcggacatg gccgggcgga tccagctata
     1261 cctgcgctcc gacgacctta aggagcctgc ctacgactgg ttcaagaagt atgtcgattc
     1321 cggcgatatt atcggagtcg agggaacgct gtttgtaacg aagaccggag agaagaccat
     1381 agccgtcgag agactgtggg tcctgtcgaa agcgatccgc accctgccgg agaaattcca
     1441 tggtctcacg gacgtggaaa tccggtatcg ccagcgctat gtggacctta tcgtcaatcc
     1501 cgaggtccga gacactttcg agaagaggtc tcgcatggtg tctcgcatcc ggacatactt
     1561 gactgagcgg ggatacctcg aggtggagac ccccatgctc cagccgatag cgggcggcgc
     1621 aaccgcgcga ccattcgtga cacatcacaa tgctctcgac caggacctct tccttcgtat
     1681 cgctccggaa ctgtatctga agagactgct tgtcggtggc tttgagaagg tattcgagat
     1741 caatcgcagc ttcaggaatg aggggatcga cactgtccac aacccggaat tcacaatgat
     1801 ggagctgtac gaagcctatt cagaccttga cggaatgatg acgctgactg aagaccttat
     1861 ccttgacctt gtcaaaacga tcacaggcgt gtcggaagtc catttcggcg aaaggctgat
     1921 caacttcagg gggccttttg cccggacgac gtttgatgat gcgatgagac gatatgctgg
     1981 cgtcggtctg gaggagctga gagatgacgg gctggcgcgt cgcaagatct ccgagtctgg
     2041 catccatatg gacaagtctt tcgagtacgc caacgtcgtc aatgagctgt tcgacaagat
     2101 ggtcgaaccc aacttcgtcg atcccacgtt cgtgcacgac taccctgtgg agatctcacc
     2161 gcttgccaag cgcatggccg aacagccaca gagagtccac cggttcgagc tctttgctgg
     2221 aggcatggag cttgccaacg ccttcactga actcaacgac ccgatcgacc aggaagagag
     2281 gtttgtgcag caagccgcag cgaaggacag gggcgacgat gaaagccagt ccatggattt
     2341 cgactatgtg cgcgctctgc aatacggcat gccgcctgcg ggtggactgg gcattggcat
     2401 cgacaggctt gtcatgctgc tgctcggcgt cgagtccata cgcgaggtga tcctgttccc
     2461 tcagctcaag agaagggagt cacaggactg acgttgggtc gacataggag ggcatgatgg
     2521 cgaagcccca ggttcagtac aggtgcagcg agtgtggaca tgtcactgtc tcaaaactcg
     2581 gaaagtgccc cgagtgcgga tcctggggtt ccttcgttga ggacattccc caggttgagg
     2641 cggtggcaag gccaccttcg agggtgctga cttccgggat actgcccgca gagatcgatc
     2701 tggaagtggc tgtggagaac gtccagccag aacggcgaat ggtgaccgga attggcccgt
     2761 gggacgaggt attgggaggc ggtctggtag taggttcagc tgtcctcgtg ggaggggaac
     2821 ccggtatcgg aaagtctacg ctcgttctgc aggcggcgac ggccttgggg tgccatggga
     2881 aggtactgta cgtcagcggc gaggagtctc gttcccagat ccttggacgt gttcgtcgac
     2941 tggggttgga tccgacgaac ctgcttcttg ccacgacgaa ctctctcgct gacgcgctgg
     3001 ccatggctga gcgagccagg ccggtggcgc tagtcatgga ctcgctgcag tcattctcga
     3061 cagaggagga cttcttcgga tccgggactc cggctcagct gaagacgact gctgctgcta
     3121 tagcggggtt ttccaagaag tccggcgtgg cggccctgat catcggacac gtgaccaagg
     3181 acggtgcaat cgcgggaccc aagtacgtcg agcatcttgt cgatgccgtc ctctatatgg
     3241 aaggagaccg gttcggggcg taccgacttt tgagatctgg gaagaatcgg tatggcaaga
     3301 gcggcgagac cgggttcttc gagatgacat cgtcagggct ggtcgcgatc tccgacccat
     3361 cgagtgcatt cgtgaccgac gtgcacaacc tccagccggg atctgtcgtg gtgcctgcgg
     3421 ctcaggggag ctttgccgtg ctcgtcgagg tgcagtcgct tgctgccacc agctatctcc
     3481 cgtctccacg ccgcatcgcg accggactgg attatggcag gctcatgatt gcgcttgctg
     3541 taatcgagcg cagagcgggc accagcgcgg gaggcaggga cgtgtatgtc aacatctcgg
     3601 gcgacttctc ggtcgaagac cgcggtatcg atctcggggt cactctgtcc ctctacagcg
     3661 caattcgaaa cgtggcgatg cgcgaaggtg tcatgccgat cgcagaagtc gggctcgcgg
     3721 gagatctgcg tccggtattc aacttgcatc gacgcatcga gctgggggca cgcatggggt
     3781 tcaaggagtt catcgtgagc gacaagtgtc gcgacgacct cgcggatctc cgcggcctga
     3841 cgttccatcg aaagcgctac gtc
//
LOCUS       DATMZD010000106         3161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_53453,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000106 DATMZD010000000
VERSION     DATMZD010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53453"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..994)
                     /locus_tag="VN478_02250"
     CDS             complement(<1..994)
                     /locus_tag="VN478_02250"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HWQ21533.1"
                     /translation="MTFVPGSVLQWGLQIVMWLQQFAWLAPVMKALSFLGTEDFFVAL
                     VPLVYWCISVEAGIDLALVLFSVNGVNGLLKLAFHAPRPYWISLDVRGLATEASYGLP
                     SGHAAIASSCWPLMARRLRRTWALWSAVILVLLISLSRIFLGVHFPTDVLGGLVVGIG
                     VLVLYETLGRPLVAKFRHATLGVQMAWSVIVPAVLLLLTWGVQSVIAHIADPTYPLWM
                     VMNRMPDDPRDWSGFVSTAGLLGGCLAGLAFQQRHARFSVAGPVMTRALRFMVGIVGV
                     LLIWRGLAVVLPQGTGLLAQIGRFVRYALTGIWTVFLAPVIFLRTGLARPPHDDD"
     gene            complement(1066..2532)
                     /locus_tag="VN478_02255"
     CDS             complement(1066..2532)
                     /locus_tag="VN478_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009144574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HWQ21534.1"
                     /translation="MGTTKRFTILHSNDMHGDFLAEMRGEEGHLIGGLSLLSGYINQV
                     RSEEKNVIYCIAGDMVQGSLIDAEYKGISTMEIMNYLSPDVATLGNHEFDYGLPHLLF
                     LEKMANFPIVNANLYITQYNKRLFQPYIVKNVDGFDILFIGILTEKVIDMLKTDNMLS
                     SFITLQEAAAEVGKICNAYKTDDIDLTVLLTHIGFDSDKELAGLLDPAWGVDMIIGGH
                     SHTILEQPAVINNILVAQAGTGTTQIGRFDITVDDDTNSIVDWTWKLVLIDNAHCQAD
                     EKLTAFIDGFKDIVDKKYGSIISRFTRTLTHPERTRETELGNLFADAIQERTGVDVVF
                     VGSGSIRKTELGPVVTLGDYLALFPYDDSITRFVVTGAQLKHAFAGFMRPENRNGEGE
                     YYQVNRAVKAVYDLTKHELVSLTLSGVPVDDSSHYSICLQGYHYKNSAVGLQLTNEEL
                     ALLEKPRLLATSGRDIVEEHLRNHQNVQTEVEGRLQYI"
     gene            2676..3005
                     /locus_tag="VN478_02260"
     CDS             2676..3005
                     /locus_tag="VN478_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012810950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HWQ21535.1"
                     /translation="MATIQDFEQLDIRTGTITMCEPFPQARKPAYKLTIDFGPDIGVK
                     HSSAQITALYGPEQLVGRRVLGVVNFPARVVAGFPSEVLVLGVYAPEGVVLICADRPV
                     PNGLRLG"
BASE COUNT          633 a          949 c          985 g          594 t
ORIGIN      
        1 gatcatcatc gtgtggcggc cgggcaagac ctgtccgcag gaagatcacc ggcgcgagga
       61 agaccgtcca gatcccggtc agagcgtagc ggacgaagcg gccgatctga gcgagcagtc
      121 cagtaccctg gggcaggacg accgcgaggc cacgccagat caggaggacg ccgacgattc
      181 cgaccatgaa tcggagagcc ctggtcatga ctggtcctgc cacgctgaag cgggcgtgac
      241 gctgctgaaa cgccaggccg gcaagacagc ccccaaggag tccggccgtc gagacgaagc
      301 cgctccagtc acgcggatcg tcgggcatac ggttcatgac catccagagg gggtacgtcg
      361 ggtccgcaat gtgcgcgatg acactctgca cgccccacgt gagcagcagc aggactgcag
      421 gtacgatcac tgaccatgcc atctgcactc ccagcgtggc gtgccggaac ttcgcgacaa
      481 gaggacgccc gagagtctcg tagagaacga gtacaccgat gccgacaacc agcccgccga
      541 gcacgtcggt cgggaagtgc acgcccagga agatccgcga gagactgatg agcaggacga
      601 ggatgaccgc cgaccacagc gcccaggtcc tgcgcaggcg acgggccatg aggggccagc
      661 acgaggaagc gatcgccgca tggccggatg gaaggccgta gctggcctca gtggccaacc
      721 ccctgacgtc gagcgagatc cagtagggcc ggggagcatg aaacgcgagc ttgagcaggc
      781 cattcacgcc attcacgctg aacaggacca gcgcaaggtc gatgcctgct tcgacgctga
      841 tgcaccagta tacgagcggt accagggcga cgaagaagtc ttccgtgcca aggaacgaga
      901 gcgccttcat gaccggtgca agccaagcaa actgctgaag ccacatcaca atctgcagtc
      961 cccactgcag gacggaaccg ggaacaaatg tcatatgcag tcctccccgt tgtcgtacgg
     1021 cgcgacccgc ctccggcttg tgccggtctg gcgcggacgc aaggcctaga tgtactggag
     1081 acggccttcg acctctgtct ggacgttctg atgattgcgc aggtgctcct cgacgatgtc
     1141 ccggccggac gtcgccagga ggcgcggctt ctcgagcagt gccagctcct cattcgttaa
     1201 ctggagcccg accgccgagt tcttgtagtg atatccctgc aggcagatag aatagtgcga
     1261 ggaatcgtcg acggggacac cgctcagggt cagcgacacc agttcgtgct tcgtgaggtc
     1321 atagacggct ttcacggccc tgttcacctg gtagtactcg ccctcaccgt tgcggttctc
     1381 cgggcgcatg aacccggcaa acgcgtgttt gagctgcgct ccagtcacga cgaaccgggt
     1441 gatcgagtcg tcgtagggaa acagcgcgag gtagtcgcca agcgtgacga cagggcccaa
     1501 ctccgtcttg cggatactgc cgctgccgac gaagactacg tcgacgcccg tcctctcctg
     1561 gatcgcgtcg gcgaacaggt tcccgagttc cgtctcccgc gtgcgttcgg ggtgagtgag
     1621 ggtccgcgtg aacctggaga tgatactgcc atacttcttg tcgacgatgt ccttgaatcc
     1681 atcgatgaat gcggtcagct tctcgtctgc ctggcagtgt gcattgtcga tgaggaccag
     1741 tttccaggtc cagtcgacga tgctgttggt gtcatcgtcc acggtgatat cgaatcgtcc
     1801 gatctgcgtc gtgccggtac ccgcctgcgc gaccaggatg ttgttgatga cagcgggctg
     1861 ctccaggata gtgtgactgt gccctccgat gatcatgtcg acaccccagg ctgggtccag
     1921 caggcctgcg agctccttgt ccgaatcgaa cccgatgtgg gtcagcagga ctgtgaggtc
     1981 gatgtcgtca gtcttgtagg cattgcagat cttccccacc tcggccgccg cctcctgcag
     2041 ggtgatgaac gaggacagca tgttgtcggt cttgagcatg tcgatgacct tctccgtgag
     2101 gatcccgatg aacaggatgt cgaagccgtc cacgtttttt acgatgtatg gctggaacag
     2161 gcgcttgttg tactgcgtga tatagaggtt ggcattgacg atagggaagt tggccatctt
     2221 ctccaggaac agcaggtgtg gcaggccgta gtcgaattcg tggttgccca gcgtggcgac
     2281 gtccggcgaa aggtagttca tgatctccat cgtgctgatg cctttgtact cggcgtcgat
     2341 cagagacccc tgcaccatgt cgcctgcgat acagtaaatg acgttcttct cctcgctgcg
     2401 gacctggttg atgtacccgg acagcagtga caggccccca atgaggtgtc cctcttcgcc
     2461 gcgcatctct gcaaggaagt caccgtgcat gtcgttgctg tgcaggatcg tgaatcgttt
     2521 cgtcgtaccc atggtgacct cccgagtgaa tggtagtgac cggttctggc gacattctcc
     2581 ggaaccgtgg ttccgtgtca agtgactgcg cgtccgacga ccattgccac gaacgcttcg
     2641 ctgcgtagta ttgaccaaaa gagccggagg cgacagtggc gacgatacag gacttcgaac
     2701 aactcgacat caggactggg acgatcacca tgtgcgagcc gtttccccag gcccgcaagc
     2761 ccgcgtacaa gctgaccatc gactttggtc cggacattgg cgtcaagcac tccagcgcac
     2821 agatcacggc tctctacgga cccgagcagc tcgtcgggcg ccgggtactg ggcgtcgtca
     2881 acttccccgc ccgtgtcgtg gccggcttcc cctcagaagt gctggtcctc ggcgtctacg
     2941 cgcccgaggg tgtcgtgctg atctgtgcag accggccggt gcccaacggg ctcaggcttg
     3001 gctgatccgg aggtgttgtg cctgagcgcg cagcgcctat catgagagag aacctcaacc
     3061 aggaggcaac tatggaagaa gacaggaaaa ccttctacat cggcaccgag gtggacccga
     3121 cgaccggtca ggccggagcc tccgtcctgt acccaggcaa g
//
LOCUS       DATMZD010000107         2837 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_123619,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000107 DATMZD010000000
VERSION     DATMZD010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123619"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1031)
                     /locus_tag="VN478_02265"
     CDS             complement(<1..1031)
                     /locus_tag="VN478_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21536.1"
                     /translation="MKRQVSRRILTFAVALVLFTSLATIHAAVPVRALSVPSVTVTPN
                     VVGERAKYTVEFTLGEPLSSGDEIWLYFPVGTYLPCTSCNPRILQTTVTVNGVYPIFP
                     SIGNATTRIVQVYVPKALKDGEKVTLIIDPSAGVRNPTNGGSYVLGVSTSTEGKRLDS
                     PAFAIGASRISNAVVHLTDSSLVGKNTGYLVDVITGYGGALVAGTGSVTLTFPEGTVI
                     PTSDEPAGIAINGVASRHIETNPVTRSVTITVPVAVNAVDLLSIALPSSFGIVNPDKR
                     GEYSLFVHTSAEIGDVRSDPYVVEDAPAVATVLAITPPAPEGEDMWYLHSPTVALNAQ
                     SNKEGALST"
     gene            complement(1112..>2837)
                     /locus_tag="VN478_02270"
     CDS             complement(1112..>2837)
                     /locus_tag="VN478_02270"
                     /inference="COORDINATES: protein motif:HMM:NF019450.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="copper amine oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HWQ21537.1"
                     /translation="PMTYTAVGSLVSVDVGVAVPAGGSVSLVMPLEVGLVNPLQQGPV
                     SIGIRTSKDTTTAVATGQIVPPSVGSASATFSTLAVGANPSLQLSFMTSQAGALSAGL
                     DTITIAFAQPFVVGKDILPASINVNGVPAAQYAVQGQELQIVVPEAVAADSAVTVAVS
                     EQAALASPTNPCSASLQLETSRDLTAVSVGPFVFREAMPVVCSMTPSRPTGQNGWYVG
                     TPPAISLDPGVGRTVWYRFDDTPFALYTGTAVVAPEGIHTLSFYGVDADGVTWEPVQQ
                     EVKVDTVKPSMTLDNAGEIHGVADGFIELTGRISEPVEVLQYNSVPAEVRSDLSFTVR
                     LPAAQQAGMLGAYARDLAGNVTTSISSLRIDKTAPRITMLSPNTPEVSVSSDILHVRC
                     TIDESGEVSVDGKEAVFDGSAWTLDVQLQEGTKSVVVEARDAVGNQSQVTLTVTYRLM
                     TDIVLTVGKSVASVGDRTVDMGVAPIIHQSSTMVPLRFVSEILGATVDWSPAMQIVTL
                     TLGRRVIQLQIVSLMALVDSSVVTLTAAPMLSDGHTLVPLRFISETLGATVAWDAVLK
                     TVTVVLAR"
BASE COUNT          625 a          855 c          814 g          543 t
ORIGIN      
        1 catgtcgaca gagcgccctc cttgttcgac tgcgcgttca acgccacggt cggcgaatgc
       61 aggtaccaca tgtcctcgcc ctcgggtgca ggcggggtaa tcgcgagaac ggtcgccacg
      121 gcgggcgcat cctcaacaac gtatggatct gagcgcacgt caccaatttc ggcagaggtg
      181 tgcacaaaca gcgagtactc gccacgcttg tccgggttca cgataccgaa agatgacggc
      241 agcgctatgc tcaacagatc cactgcgttc acagcaacgg ggaccgtgat ggtcacactg
      301 cgggttaccg ggttggtctc aatatggcgg gaagccacgc cattgatggc aattcccgct
      361 ggttcatccg aagtcggaat cactgtacct tccgggaatg tcagtgtcac gctgcctgtt
      421 ccagccacaa gagctccacc gtagcctgtg atgacgtcga caagataccc ggtattcttc
      481 ccgaccaggc tcgagtccgt cagatggacg actgcgttcg agatgcggct ggcgccgatc
      541 gcgaacgctg gagagtcgag tcgtttgccc tctgtcgatg tcgacacacc aagcacgtac
      601 gatcccccgt tcgtcggatt gcgcactccc gctgatggat caatgatcag cgtcaccttc
      661 tcaccgtctt tcagcgcctt cggaacgtag acctggacga tgcgagtagt cgcgttgccg
      721 atcgatggga agatcggata gactccattg accgtgactg ttgtttgcag gatccgggga
      781 ttgcacgacg tacacggaag atacgtgccc acagggaaat agagccaaat ctcgtcgccg
      841 ctgctgagag gctcgccaag ggtgaactca acagtatact tcgcccgctc ccccaccacg
      901 ttgggggtca ccgtgacact cggaaccgac agcgctcgca caggaacagc agcatgtatt
      961 gtcgcaaggc tggtgaaaag caccagtgcc acagcaaacg tcaggatcct cctggagact
     1021 tgcctcttca tctgttgcct ccttggcaat tcatgtgttt tctagcagac agaacaaaag
     1081 tcgtggacgt cagcggaaca ggtactcgcc gctatcgtgc cagcacgact gtcacggtct
     1141 tcaggacagc atcccacgct acagtagcac ccagcgtctc actgatgaac ctcagcggga
     1201 ccagcgtatg tccgtccgac agcatcggag cagcggtcag tgtgaccacg ctgctatcga
     1261 ccagtgccat caacgagact atctgcaact ggatgacgcg ccttcccaga gtgagagtca
     1321 cgatctgcat ggccggactc cagtcgaccg tcgctccgag aatctcggag acgaatcgaa
     1381 ggggtaccat cgtgctcgac tgatggatga tgggggccac tcccatatcg acggtccgat
     1441 caccgacaga tgccacgctc ttgcctactg tcagcacgat gtccgtcatg agccgatagg
     1501 tgactgtcag cgtcacttga gactggttgc cgactgcgtc acgagcctcc acgaccacgg
     1561 actttgtgcc ttcctgcaac tgaacgtcca gggtccaggc actcccgtcg aagaccgcct
     1621 ctttgccatc gacactcact tcgcctgatt cgtcaatcgt gcaccgcaca tggagaatgt
     1681 cggatgagac actgacctcg ggcgtattcg gcgatagcat cgtgatgcga ggtgccgtct
     1741 tgtcaattct gagggaagag atcgacgttg tgacattgcc cgccaggtcc cgggcatatg
     1801 cgccaagcat gccggcttgc tgagcggcag ggagacgaac ggtgaacgac agatcgctgc
     1861 gaacctcggc aggcacgctg ttatactgga ggacctcgac gggttcgctg atgcgtcctg
     1921 tgagttctat gaacccatcg gccacaccgt gtatctcccc tgcgttgtcg agagtcatcg
     1981 atggcttgac cgtatcgacc ttgacttcct gttgcaccgg ttcccacgta acgccatcag
     2041 catcgacgcc gtagaaggac agtgtgtgta tcccttcggg ggctacgacg gcagtgccag
     2101 tgtagagagc gaaaggcgtg tcgtcaaaac ggtaccagac ggtgcggcca accccaggat
     2161 ccagcgagat cgcagggggc gtgccaacgt accatccgtt ctggcccgta ggccgggaag
     2221 gtgtcattga gcacacgacc ggcatcgcct cacgaaacac gaatggaccc accgaaacgg
     2281 ccgtgaggtc acgcgacgtt tcgagctgga gagatgcact gcagggattc gtggggctgg
     2341 ccagcgccgc ctgctccgag actgcgaccg tcaccgccga atctgctgca acggcttccg
     2401 gaaccactat ctgcaattcc tggccctgaa cagcgtactg tgccgcagga actccattaa
     2461 cattgatgga agccggcagg atatccttgc ccacgacaaa cggctgtgca aaagctatcg
     2521 tgatcgtgtc gagaccagcc gagagagctc cggcctgact cgtcatgaac gacagctgca
     2581 gcgaaggatt cgcgccgaca gcaagcgtag agaatgtggc agacgcgctg cctacagacg
     2641 gcggcacgat ctggccggtc gctacagcgg tagtggtatc cttgctcgta cggataccaa
     2701 ttgacactgg accctgctga aggggattga ccagcccgac ctcaagcggc atgaccaggg
     2761 agacagaccc acccgccgga acagcaaccc caacgtcgac tgaaacaagc gagccgacgg
     2821 cagtatatgt cataggc
//
LOCUS       DATMZD010000108         4296 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_13186,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000108 DATMZD010000000
VERSION     DATMZD010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4296
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13186"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..898
                     /locus_tag="VN478_02275"
     CDS             <1..898
                     /locus_tag="VN478_02275"
                     /inference="COORDINATES: protein motif:HMM:NF012945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21538.1"
                     /translation="AEHGASFDEWTPLAPGMTLDVLDPVFDHFGLPDTTKYVLIDVTA
                     TAETGPLLLEALAMGIPVVTANKIPLAETPGWFEQARAASQNARTPFRYECTVGGSLP
                     VISTLVDLMTTGDTVQRIVALASSSLSCIMGDFNRGMPLEAAIADAMRRGFAEPNPLT
                     DIAGRDALRKIVILAKTMGLPTGVGDVDQEPLLDQDYPDFEAFQGEGMARLAARLTAA
                     RKPGTVTYYVGSVSADGAVTLGLRSFPQASIWGTLEPSENIFLFSTSRFGDLPVVVRG
                     VGGGPTLTASGVLADVIKAARG"
     gene            complement(912..2105)
                     /locus_tag="VN478_02280"
     CDS             complement(912..2105)
                     /locus_tag="VN478_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21539.1"
                     /translation="MSRAHRGNTREIWRRVVPVCLVLTAVIMAFVVTHQPLARVEVVD
                     FAKEQQNLGSIYGLWTDEQKALKAMPLVDYIVSVTAGKAMAANGTTWDAFARGVSLTQ
                     EGSPPDRTWRQRVGEWDTVYVRQTELPSPEMLAGLPDGEVRYAALPDGTWVRAVAVRL
                     SSSDFTLGAGLHGPPHALVYPLRGLFFPLLGLAVLFYVLLPWPRRGEDVIAYDRWRVV
                     LGDILGLVLTVMFVAMPFLITSNSALAVTEYWGLTLVFWLIGLLGFWAVQVGISAGGL
                     QMMVLADRLVRVTAWRHEDCAYADIALVQPAIQVAPKWLITASLLAALSGRRGSGAVL
                     LGAQSETRGFRITMKDGSLVHIWLTDQMGSVMIRGADALPRALAAAGVPMSTESVTVR
                     GMSVG"
     gene            complement(2181..2876)
                     /locus_tag="VN478_02285"
     CDS             complement(2181..2876)
                     /locus_tag="VN478_02285"
                     /inference="COORDINATES: protein motif:HMM:NF024873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HWQ21540.1"
                     /translation="MERLVFDIETVGAAWESLDQAVQESLLRPADTDEERQEVRDSLG
                     LFPVTAQIVCVALYSPEQDHAAVYFQAPNGGVETVREEKAVFVPCTEKELLAHFWEAA
                     GRAKQVITFNGRGFDCPFLLVRSAANRVRPTIDLMPNRYSNQHVDLMDQFTWFGAFRR
                     RFSLEVWCRALGVEQPKREVHGSDVSALYADGEYMKIAQYCLGDAMATARLFDLWSKY
                     MRYPGPEQERPPR"
     gene            complement(2879..3787)
                     /locus_tag="VN478_02290"
     CDS             complement(2879..3787)
                     /locus_tag="VN478_02290"
                     /inference="COORDINATES: protein motif:HMM:NF006615.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HWQ21541.1"
                     /translation="MVDDERMQWDTIAQALEETGEYRVIHKLRPVNRYSEPDGEPTQV
                     GLVLDTETTGLDPQTEEVIELGMVLFEYAPATGRIYRILDSFDELRDPGRHLSAEITA
                     LTHITDDMVRGHTIDVAAVEAFVSRAALVIAHNAAFDRPFVEKQWDVFATKPWGCSMT
                     QVHWRDEGIATQKQELIALCQGFFYDAHRAENDCRALLHILSTPLPVSGQPALKPLLD
                     HAMANDAHIWAIAAPFDAKDVLKTRGYRWSTGTGGDPKAWHITVMADELEAELVFLDA
                     IYGGDARSIIRVDRMSPLTRFSARRE"
     gene            complement(4005..>4296)
                     /locus_tag="VN478_02295"
     CDS             complement(4005..>4296)
                     /locus_tag="VN478_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21542.1"
                     /translation="HCFVNMEPYPTPNIWQQDVVRVLEAVDFVDEIRLGQLNYNDVAK
                     QYPGWRAWYRQQGAAARDWCGAHGIQYDGIEARPSPRRKRRADSDTGDLFGS"
BASE COUNT          767 a         1435 c         1320 g          774 t
ORIGIN      
        1 ggccgaacac ggagcatcct tcgacgaatg gacgcccctc gcgcccggta tgacactcga
       61 cgtcctggac cccgtgttcg accacttcgg gcttccagac accacgaagt acgtgctgat
      121 cgatgtcact gccaccgctg agacgggacc cctgctgctg gaagccctgg caatgggcat
      181 cccggtcgtc acggcaaaca agatccccct ggctgagacg cccggctggt tcgagcaggc
      241 gcgggctgca agtcagaacg cacgcacgcc gttccgctac gagtgcacgg ttggaggttc
      301 cctccccgtc atcagcacgc ttgtcgacct catgacgacc ggcgacacgg tccagcgaat
      361 cgtcgctctg gccagcagtt cactgtcatg catcatggga gacttcaacc gtgggatgcc
      421 attggaagcg gctattgcag acgcgatgcg gagggggttt gcagagccca atcccctgac
      481 ggacatcgcc gggcgcgacg ccctgcgcaa gatcgtcatc ctggccaaga cgatggggct
      541 ccccaccggc gtcggcgacg tcgaccagga accactgctt gaccaggact acccggactt
      601 cgaggccttc cagggcgaag gcatggcgcg tctcgccgca cgtctgacag ccgcacgcaa
      661 gcccgggaca gtgacgtact acgtcggctc ggtctcggct gacggagctg tcacgctcgg
      721 cctccgcagc ttcccgcagg cgtccatatg gggcacgctg gaaccttccg aaaacatctt
      781 cctgttcagc acgagccgat tcggcgacct cccggtggtc gtccgcggcg tcggcggagg
      841 tcccacattg accgcctcag gtgtcctggc agacgtcatc aaggcagcac gggggtagat
      901 ccaggagacc tctacccgac gctcatcccc cgcaccgtga cactctcagt gctcatgggg
      961 actcccgctg cggcgagcgc gcgaggcaag gcgtccgcgc cacggatcat caccgaaccc
     1021 atctgatccg tcagccagat gtggaccagg ctcccgtcct tcatggtgat gcgaaagcct
     1081 cgcgtctccg actgcgcgcc cagcaggacg gcgcccgaac cccggcgccc tgacagcgcg
     1141 gcgagcagcg atgctgtgat cagccacttc ggtgccacct ggatggcagg ctggaccagc
     1201 gcgatgtctg cgtacgcaca atcctcgtgt cgccacgccg tcacgcgcac gaggcggtcc
     1261 gccagcacca tcatctgcag gcccccggcg ctgatgccga cctgcacggc ccagaagccg
     1321 aggagcccga tgagccagaa caccagggtc agtccccagt attcagtgac cgccagtgca
     1381 gagttgctgg tgatcaggaa tggcattgcg acaaacatga ctgtcaggac gagtcccagg
     1441 atgtcgccga ggactacgcg ccatcgatca tacgctatga cgtcctcgcc cctgcgcggc
     1501 cacggcagca ggacgtagaa cagcacagcc agccccagga gagggaagaa cagaccgcgc
     1561 agaggataca cgagcgcatg cggaggtccg tgcaggccag cgcccagcgt gaagtcgctg
     1621 ctgctgaggc gcaccgcgac ggccctcacc catgttccgt caggcagagc cgcgtagcgc
     1681 acttccccgt ccgggagacc ggccagcatt tccggtgatg gaagttcggt ctgccggacg
     1741 tacacggtgt cccattcgcc gacgcgctgc cgccaggtgc ggtctggagg gctcccttcc
     1801 tgtgtcagcg acactcccct ggcaaacgca tcccatgtcg tgccgtttgc ggccatcgcc
     1861 tttcccgcgg tcaccgacac gatgtagtcg acgaggggca tggctttgag cgctttctgc
     1921 tcgtcggtcc acagcccata gatgctgccc aggttctgct gctccttggc gaaatcgacg
     1981 acctcgaccc gggccagcgg ctgatgagtc actacgaagg ccatgatgac ggccgtcagc
     2041 accaggcata ccggcaccac gcgacgccag atctctcggg tgtttcctct gtgtgccctg
     2101 ctcatccgat gcactcctcc ctgcgaagtc tctctccttc catcatgggg aaatacgcag
     2161 gtgctgcaag aggaacggcg ctatctcggc ggcctctcct gctccggacc cgggtaccgc
     2221 atgtacttgc tccacaggtc gaacaggcgc gcggtcgcca tggcgtcacc gagacagtac
     2281 tgcgcgatct tcatgtattc gccgtcggcg tagagtgctg agacgtcgct cccgtgcacc
     2341 tcgcgcttgg gctgctcgac gcccagcgcc ctgcaccaga cctcaaggga gaagcgacgc
     2401 cgaaacgccc caaaccacgt gaactggtcc atcaggtcca cgtgctggtt gctgtagcga
     2461 ttgggcatga ggtcgatggt cggacgtacg cgattggcgg ccgaccgcac gaggaggaag
     2521 gggcagtcga atccgcgccc gttgaacgtg atgacctgct tcgccctgcc ggccgcttcc
     2581 cagaaatgtg ccaggagctc cttctcggta cacggcacga acaccgcctt ctcctcgcgg
     2641 acggtctcca cgcccccgtt cggcgcctgg aagtagaccg cggcgtggtc ctgttccggc
     2701 gagtacagtg caacgcacac gatctgtgcc gtgacgggga agagtcccag cgagtcacgc
     2761 acctcctggc gctcttcgtc cgtgtcggca ggccgcaaca gcgactcctg gactgcctga
     2821 tccagtgatt cccaggccgc tccgaccgtc tcgatgtcaa agaccagtcg ttccatacct
     2881 actcccgccg tgccgaaaag cgtgtgagtg gcgacatccg gtctacccgg atgatactgc
     2941 gcgcgtcacc accgtagatg gcgtccagga acaccagctc agcttccagc tcatccgcca
     3001 tcaccgtgat gtgccaggct ttggggtcgc ctccggtgcc cgtgctccag cgatacccgc
     3061 gcgtcttgag gacgtccttg gcgtcgaacg gcgcggcaat ggcccagatg tgggcatcgt
     3121 tggccatggc atggtcgaga agaggcttca gggcgggctg gcccgagact ggcagcgggg
     3181 tcgacaggat gtggagaagc gcccggcagt cgttctcggc acggtgagca tcgtagaaga
     3241 atccctggca gagcgcaatg agttcctgct tctgggtcgc gatgccctcg tcacgccaat
     3301 gcacctgggt catggagcat ccccacggct tggttgcgaa cacgtcccat tgcttttcga
     3361 caaacggacg gtcaaacgcc gcgttgtgcg cgatgaccag ggcggcccgt gacacgaatg
     3421 cttcgaccgc agccacgtcg atggtgtgcc cccgcaccat gtcgtcagtg atgtgcgtca
     3481 gggccgtgat ctcggcagac aggtgacgtc cggggtctct caactcgtcg aagctgtcaa
     3541 ggatgcggta gatgcgcccc gtggcgggcg cgtactcgaa caggaccatg cccagctcga
     3601 tgacctcttc cgtctgtgga tcgagacctg tcgtctcggt atcgaggacc aggcccacct
     3661 gggtcggctc gccgtcgggc tcactgtatc ggttgaccgg acggagtttg tggatgacgc
     3721 ggtattcgcc cgtttcctcc agtgcctgcg cgatcgtgtc ccactgcatt cgttcatcgt
     3781 ctaccattgc ttccccccga gcactttccc ccaatgcccc cgagcatgtt ggtccaatgc
     3841 gaggggcgct cctgaggggc gtactctcca cgtgataccg cgttgccgcg cattgttgtt
     3901 atatcacgat tccgtctgtt gccgtcgtac cagagggccg cacctcacat gtcagatcgt
     3961 accggaggcc ctttggcaca agatggtaga tgttacagac caggtcagga gccgaacagg
     4021 tcgccagtgt ccgagtctgc gcgccgtttg cgacggggcg aggggcgcgc ctcgatgccg
     4081 tcatactgga tgccgtgggc tccacaccag tcgcgggccg cggcgccctg ctgacggtac
     4141 catgcacgcc atccgggata ttgcttggcg acgtcgttgt aattgagctg acccagccgg
     4201 atctcgtcga cgaagtcgac cgcctcgagg acacggacga cgtcctgctg ccagatgttg
     4261 ggggtcgggt acggctccat gttcacaaaa cagtgg
//
LOCUS       DATMZD010000109         2321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_73076,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000109 DATMZD010000000
VERSION     DATMZD010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73076"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /gene="alr"
                     /locus_tag="VN478_02300"
     CDS             <1..888
                     /gene="alr"
                     /locus_tag="VN478_02300"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HWQ21543.1"
                     /translation="LVFGYVPASGVQEAIAQHITLSVPNDDVARELVEAADGKRLSVH
                     LKVDTGMGRGGVLAEGAMSVLRNLSGMSDVDVEGIYSHFSCADSDESYSSMQLRRFEK
                     ILHQATVEGMRPPLAHFCNSAGILTMKAATFEGVRPGLLLYGCSPLEGVPITGLRPAM
                     SMRTRVIALKEVPAGYGISYGRSYLTYKPTTVGILPVGYTDGYSRMLSNKASVLICGK
                     RAPVIGRVTMDQTMIDVSDVRGVKVGDEAVLMGEAGSQRITAGDLGSLAQTISYEVLC
                     TVGKLVRRVYSDRPDPESE"
     gene            906..1712
                     /locus_tag="VN478_02305"
     CDS             906..1712
                     /locus_tag="VN478_02305"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HWQ21544.1"
                     /translation="MPVQEALQGILASVAFPCAVYAELLTGEDRCLIFSQNESLPFGA
                     ASIIKIPILVCVAQLVEQGELTWDQSIRLTVPGPHGTGLLEHLDDSRPWSVQDLCIMM
                     MGVSDNMACNQIVEEIGMERLNGLLSGLGFEATRIRRRMMDHETLARGVDNTVTAVEI
                     ADMLARIDARRLVSSAASERLLGYLRMNQLKDLIAWPLPGSVVLAGKTGGMPGSLLDA
                     ELVSVGGGMTYSLCVFTSGFERALQAKRVIAQVSELVYSAVSGNRSADCV"
     gene            complement(1793..>2321)
                     /locus_tag="VN478_02310"
     CDS             complement(1793..>2321)
                     /locus_tag="VN478_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21545.1"
                     /translation="YITRAAVILALTIALQFGIREIIPSVPPFNFINLFVVGSIVNLG
                     LLLATETTGLWAGVVIALAAPVTAWLQQHLPSPAMIPAVMAGNIILVLVYWLATRRSG
                     GQSWMRWVGLLVGGAAKMAFLYYAIGAIVGTLSALPPTAAAFIRFSFSWPQLVTAVIA
                     GFLSALIAGRIKPLT"
BASE COUNT          458 a          644 c          777 g          442 t
ORIGIN      
        1 ctcgtgtttg gctacgtgcc cgcatcggga gtccaggagg cgattgcgca acacatcaca
       61 ctcagtgttc ccaacgacga cgtggcacgt gaactcgtcg aagcggcgga tgggaagcgg
      121 ctgtcagttc atctcaaggt agacacgggg atgggaagag gcggcgttct tgcggaaggc
      181 gcgatgagcg tactgcgcaa tctgagcggg atgagcgacg ttgacgtgga ggggatctac
      241 tcgcatttct cctgcgcaga ttccgacgag tcatattctt ccatgcagtt gcgcaggttc
      301 gaaaagatcc ttcatcaggc gaccgtcgag ggtatgcggc cacctttggc tcacttctgc
      361 aactcggcgg ggatcctgac catgaaggcg gccaccttcg aaggggtccg tccagggctg
      421 ctgctctacg gctgttcgcc ccttgagggc gtgccgatta ccggattgcg gccggcgatg
      481 tccatgagga cccgggtgat cgcactcaaa gaggtccctg ccggctacgg catcagctat
      541 gggcggagct acctgacata caagccgacg acggtgggga tccttccggt cgggtacact
      601 gacggatact ctcgcatgct gtcaaacaag gcctccgtgc tcatctgtgg aaagcgagct
      661 ccggtcatag gcagagtgac catggaccag accatgatcg acgtgtcgga cgttcgaggc
      721 gtgaaggtgg gcgacgaggc agtgctcatg ggcgaggccg ggagtcagcg catcactgcc
      781 ggggatctgg ggtcgctcgc ccagacgatc agctatgagg tgctctgcac cgtcggcaag
      841 ctggttcggc gcgtgtacag cgaccgtccg gatcccgagt ctgagtgaag caggacaccc
      901 gttacttgcc cgtccaggaa gcgctccagg gaatcctggc gtcggttgcg tttccatgtg
      961 ccgtgtacgc ggaacttctg accggagaag accgctgcct catcttctcg caaaatgaga
     1021 gtctcccgtt tggagcggcg tcgatcatca agatcccgat ccttgtttgc gttgcacagc
     1081 tggtggagca gggtgagctg acgtgggacc agtccattcg actgacagtc cccggccccc
     1141 atggaactgg tctgctcgag catctcgacg actcgcgccc atggtcggtc caggacctgt
     1201 gcatcatgat gatgggcgtg tccgacaaca tggcgtgcaa ccagattgtc gaggagatcg
     1261 gcatggagcg tctgaacggt ctcctgagcg gtcttggttt cgaggcgact cgtatccggc
     1321 gtcgaatgat ggaccatgag actctggctc gcggagtcga taacacggtg accgctgtcg
     1381 agattgccga catgctggcg cggatagatg cacgtcgcct ggtctcaagc gcggcgagcg
     1441 aacgcctgct tggctatctg cggatgaacc agctgaagga ccttattgcg tggccgctcc
     1501 ctggcagtgt ggtgttggcg ggaaagacgg gcgggatgcc cggttctctg ctcgacgccg
     1561 agcttgtgag cgtcggaggt gggatgacat actcactgtg cgtgtttaca tcaggattcg
     1621 agcgcgcatt gcaggcgaag cgtgtcatag cgcaggtgtc cgaactcgtg tacagtgctg
     1681 tctccgggaa tcggtcggcg gactgcgtct gacacggctg cttcacgcag aaagaacgca
     1741 catgacaacg ccccgcgtca gcggggcgtt tctcttgagc agaacttctg gatcaagtca
     1801 gaggcttgat ccgaccagca atgagagccg agaggaagcc cgcgatcacg gcagtgacca
     1861 gctgcggcca gctgaacgag aacctgatga aggctgcagc ggtggggggc agagcggaca
     1921 gcgtgcctac gatggctccg atggcatagt agaggaacgc catcttcgct gcgccgccga
     1981 ccaggaggcc aacccaccgc atccaggact gaccgccgct ccttcgcgtg gcgagccaat
     2041 agacgaggac aaggatgatg ttgcccgcca tgacggcggg gatcatggct ggggacggga
     2101 ggtgctgctg gagccatgcg gtgacaggcg ccgcaagggc gatgacgacg ccggcccaga
     2161 gcccggtcgt ctcagtcgcg aggagcagcc cgagattgac aatactccca acgacgaaca
     2221 ggttgatgaa gttgaagggc ggcacggatg ggatgatctc cctgatgccg aactgcaggg
     2281 cgatggtcag tgccaggatg accgcagctc ttgtgatgta c
//
LOCUS       DATMZD010000110         3601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_43200,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000110 DATMZD010000000
VERSION     DATMZD010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43200"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            7..399
                     /locus_tag="VN478_02315"
     CDS             7..399
                     /locus_tag="VN478_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HWQ21546.1"
                     /translation="MQGPVTITMLGMQGDAHAGDWHRQISLLAQESIDKMQGHGLVLQ
                     PGDFAENITTEGLDLLTLPIGTHLQIGTIVTMEVTQIGKECHLGCEIRKLVGDCVMPR
                     EGIFARIITPGMIEAGDIITIISRGGTP"
     gene            396..872
                     /locus_tag="VN478_02320"
     CDS             396..872
                     /locus_tag="VN478_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015043691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HWQ21547.1"
                     /translation="MTAAVLTVSDSCAAGTRVDESGPFIAGRLQGAGWTIHEMTIVPD
                     EQMEIADILEKWAAEHHVDAIITTGGTGFSLRDVTPEATLSVVDRRAPGISEAIRLYG
                     LNKGVPTACLSRAEAGLRASTLIVNLPGSLKAVQDGMEVLIPLLPHALEMIAGKGH"
     gene            complement(905..1324)
                     /locus_tag="VN478_02325"
     CDS             complement(905..1324)
                     /locus_tag="VN478_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007156957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HWQ21548.1"
                     /translation="MEYTRSGDLIALRVNRGEEIISCVKAVCEKEQVHFGSISGIGAV
                     DHVVVGLYHVADKRYESNTFDGEVEMTSLLGNATEKDGQVYLHFHAAFAKEDGQVVGG
                     HLNEARVSGTAEIFIHTAPGSIGRKADPVTGLNLFDF"
     gene            complement(1652..2506)
                     /locus_tag="VN478_02330"
     CDS             complement(1652..2506)
                     /locus_tag="VN478_02330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HWQ21549.1"
                     /translation="MKKRESWEFLKDLYVRFGNDDLLSTGAEVAFYLLLSLFPFLVFL
                     IALISYAPMVDIQDSIQILAPLMPASAYEILQGIVNQTIADRSGTLLSFGMVLAVWSS
                     ASGVTSLMSGINRAYDQEETRRSWRVKGVSLLFTLELAAVIAVSFVLIVLGKNLGTRV
                     FHFLGLSDISLEIWIYVRFIIVTITSILVFVSFYHNTPNRRMSFREVIPGATVASLGW
                     VAVSTGFSYYVNNLGSYSKVYGSLGGIIALLMWMYLSSISILMGAEVNASLMFTRTGG
                     EKPKSRRF"
     gene            2650..2790
                     /locus_tag="VN478_02335"
     CDS             2650..2790
                     /locus_tag="VN478_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015988143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21550.1"
                     /translation="MPLKKGYGKKTIGENIKTEIKAGRPQKQAVAIALSVARKAKRSH
                     GK"
     gene            2967..>3601
                     /locus_tag="VN478_02340"
     CDS             2967..>3601
                     /locus_tag="VN478_02340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HWQ21551.1"
                     /translation="MRPYTECMKYYIIDAFTDKLFAGNPAAVCLLQEPLTDEAMASIA
                     REMNLSETAFVLWESGAWRLRWFTPAVEVPLCGHGTLATARALREAGIVKGDEVYFRS
                     LSGDLSARYQGDWIELDFPSLPPVSTPVPDGLLKALKIHTLPRWTGVNSHHYWLLDVE
                     SAATVCSLTPNRAATMALDVPGIIVTAQGDAPYDFTSRFFAPADGIFEDPVT"
BASE COUNT          780 a         1094 c         1013 g          714 t
ORIGIN      
        1 agacagctgc agggtcctgt cacgatcacc atgctgggca tgcagggcga tgcccacgca
       61 ggcgactggc atcgtcagat cagcctgctt gcacaggaaa gcatcgacaa gatgcagggt
      121 cacggcctcg tgctgcagcc cggcgacttt gccgagaaca tcacgacaga gggcctggac
      181 ctgctgaccc tgccgatcgg gacgcatctc cagatcggca ccattgtcac gatggaggtc
      241 acccagatcg gcaaggagtg tcacctgggc tgtgagatcc gcaagctggt cggcgattgt
      301 gtcatgcccc gcgaaggcat ttttgcccgc atcatcacgc caggaatgat cgaggccggc
      361 gacatcatca cgatcatcag tagaggagga accccatgac agcagcagtc ttgaccgtca
      421 gtgacagttg tgcggcaggc acccgcgtcg acgaaagcgg gccattcatc gctggacgcc
      481 tgcagggtgc aggctggacg atccatgaga tgaccatcgt accggacgag cagatggaga
      541 ttgccgatat cctcgagaaa tgggcggccg agcaccacgt cgacgccatc atcacgaccg
      601 gcggcaccgg cttctccctc cgcgacgtca cgcccgaggc aaccctgtcc gtcgtggacc
      661 gccgtgcacc cggcatcagc gaagccatcc gcctctacgg cctgaacaag ggtgtcccga
      721 cagcctgcct gtcccgcgcc gaagccggcc tgcgagccag caccctcatc gtcaacctcc
      781 ccggcagtct gaaggcggtc caggacggca tggaagtcct gatccccctg ctcccccatg
      841 ccctggagat gatcgcgggg aagggacact aaccggtcgt aaccgctgct ccatacctgc
      901 gggactagaa atcgaacagg ttcagtccgg tgactggatc agccttcctg ccgatcgatc
      961 ccggtgcggt atggatgaag atctccgcag tcccgcttac tcgggcctcg ttcaggtggc
     1021 ctccgacaac ctgcccatcc tcttttgcaa acgctgcatg gaaatgcaga tagacctggc
     1081 catccttctc cgttgcgtta ccgagaagag atgtcatttc cacttcgccg tcaaaggtgt
     1141 tggactcgta tctcttgtct gcgacgtggt acagaccgac aacgacgtga tccaccgcgc
     1201 cgatcccgga aatgcttccg aagtgaacct gctccttttc gcagactgct ttcacacagg
     1261 agatgatctc ctcaccgcgg ttaacgcgca gagcgatcaa atccccgctt cttgtgtact
     1321 ccatggtaca gacctccttt gctttcaggc tggattcggt tggtgacgac agctttccag
     1381 tccagtatca ttctacgcga acgtgcaggt tgtcatcgct ctttcccgcg atgatgcgtg
     1441 agcgtttccg cagctgcttg ctcgtgttca cttttccccc tccacctaac gaaacttcgt
     1501 cgccatcacc ctcggcgcgc cgtatccata cggcgtcgca tcggaggtca taccctacac
     1561 tatgagtacg acgcgatacc gatgtcttga ctaacctgct gggggagaag gccagagata
     1621 cctgcgcagt ccttcctgat catgcatgac tctagaatcg tctggacttc ggcttttctc
     1681 ctcctgtcct cgtgaacatg agggaggcgt tgacttcagc gcccatcagg atgctgatgc
     1741 tgctcaagta catccacatc agcaatgcaa tgatgccgcc aaggctgccg tataccttcg
     1801 aatagctgcc caggttgttg acgtagtacg agaaaccggt agagaccgca acccagccga
     1861 gcgatgccac cgttgcaccc ggtatgacct ctctgaagct cattcgacgg ttcggggtat
     1921 tgtggtagaa ggacacaaac acaagaatgc ttgtgattgt cacgatgatg aatctcacgt
     1981 agatccagat ctcaagggat atgtccgaaa gacccaggaa gtggaagact cgtgtgccca
     2041 ggttcttccc aagaacgatc aggacaaacg aaacagcaat cacggcggcc agttcaagcg
     2101 taaagagcaa tgagacaccc ttgactctcc aggagcgacg cgtctcttcc tgatcatatg
     2161 ctctgttgat accactcatg agagaggtaa cgccgctggc ggatgaccac actgcgagca
     2221 ccatcccaaa ggacagcagg gtcccgctcc tgtccgctat ggtctggttg acgataccct
     2281 gcaggatctc atacgcactg gcaggcatga gcggagccag gatctggatg ctgtcctgga
     2341 tgtcgaccat tggagcatag gagatgagcg cgatcaggaa cacgagaaat gggaagaggg
     2401 acaggagcag gtagaaggct acctctgccc ctgtagaaag gagatcgtcg ttcccgaatc
     2461 tcacgtacaa gtccttgagg aactcccagg attctctctt cttcatggcg ctatctccag
     2521 gcaagccaac cgctgtctga ttggagtata gccaaatccc gagatctgcg tctgagatac
     2581 agtcgcggtc tgtctgctct ccgttgcacg ctctcgtttc gtgtacaata ccaagcaggg
     2641 aggtttgaca tgccattgaa gaaggggtac ggcaagaaga cgattgggga gaacatcaag
     2701 accgagatca aggccggccg tcctcagaag caggccgttg cgatcgcact ttcggtggca
     2761 aggaaggcaa agaggagcca cgggaagtag caggacgcgt gcggacagcc tggaccgccc
     2821 agctgcgccc tcgcagacca ccacctgacg ccggcatcac agtccttgtc agcagcgtca
     2881 gatgcgacga tcgacacact ctcactgtga acgctgaaga cagcagcggg tgttgtcgga
     2941 gcggacctca ccttgcacga ggacgtatgc ggccctacac tgaatgcatg aaatactaca
     3001 tcattgacgc gtttacggac aagttgtttg ctggtaatcc ggcagcagtg tgcctcctgc
     3061 aggaaccgct gacggatgag gctatggcct ccatcgcacg ggagatgaac ctgtcagaga
     3121 cagcgttcgt gctgtgggaa agcggtgcct ggcgcctgcg gtggttcacg ccggcggttg
     3181 aagtgccgct gtgcggccat gggacactcg ctactgcccg tgcactccgt gaagccggga
     3241 ttgtgaaagg cgacgaagtg tacttccgca gcctgagcgg ggacctttcc gcccggtacc
     3301 agggcgactg gatcgagctc gacttcccgt ccctgccccc agtgagcaca ccggttccgg
     3361 acggcctgct caaagccctg aagatccaca cgctcccgcg ctggacaggc gtcaactccc
     3421 atcactactg gctgctcgac gtcgagtcgg cggcgaccgt ctgttcgctc acgccgaatc
     3481 gtgctgccac catggcgctc gacgtacccg gcatcatcgt gacggcacag ggagacgcac
     3541 cgtatgattt cacgtcccgc ttctttgcgc ccgctgacgg tatcttcgaa gacccggtca
     3601 c
//
LOCUS       DATMZD010000111         5677 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_134393,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000111 DATMZD010000000
VERSION     DATMZD010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5677
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134393"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(348..1391)
                     /gene="csaB"
                     /locus_tag="VN478_02345"
     CDS             complement(348..1391)
                     /gene="csaB"
                     /locus_tag="VN478_02345"
                     /inference="COORDINATES: protein motif:HMM:TIGR03609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046919 - pyruvyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase CsaB"
                     /protein_id="HWQ21552.1"
                     /translation="MNRVLVLGYYGFGNFGDELILAAMQDELSGVQCEAVFAVKDPGQ
                     YAGATCPRHSFVDRSDMAAMRAALAACDHVMLGGGGLVQDVTSWRSSMYYLGIPLLAL
                     LHRKAVISYAQGIGPVRRPWIRRLVRTVFDRMSLIDVRDDASRELLLRLGIRAQEISV
                     SSDAGLSHLIALRPEHVVPDRRQPRIVACVTSRFGWTPEESASFLDCLGSQAGAQLDL
                     VVLFPSADLGFTRAVRNRLLMPSELIISPEPLALLELCGSASLTVAGRYHMAAAAVAA
                     RSPLVALAYDPKVSQLADCCGFNALPPGNGPQQAARWVLGGTVSPVTEAAILRFRQAR
                     IDRVARLRLLLER"
     gene            complement(1392..2858)
                     /locus_tag="VN478_02350"
     CDS             complement(1392..2858)
                     /locus_tag="VN478_02350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HWQ21553.1"
                     /translation="MTERVHVAGIPVDNLDMDEALAAVEGFVVSRAPHMGVAINPEKI
                     IKAKQDSALEKVLRKSDLNFCDGIGVMWAARLFYHERIKSRVTGVDLFLRLLDLADAR
                     EWRLFLLGSRPEVLSRVVAIVRERYPRLTVVGSRDGYFAEEDEPGLVAGIAALKPDLM
                     FVGMGSPKQEKFLAANLAAMGVPFAMGVGGSYNVLSGEFKRAPARVQRLGLEWLYRFV
                     LDPKRLPRILSLPRFVSIVIRSPRRHVDSMDFFGISISNRDMDELLAIADEFVKSAVP
                     HLVVTLNGEMAARAFRDTEFLEIVRQADLVVADGVGIVWGARILGSRIENRIPGIEFS
                     SSLLALAERRGYRVYFLGAKPEIVQRASSNMSARYPGLQVAGSHSGYFDDAQEADVIR
                     EIRAAHVDILLVGMGGGAQEKWIWRHRDIGVPVAIGVGGTFDVWSGLVRRAPRLVQKT
                     GTEWLYRLVMQPSRVRRVGSIFYFMFRVLAHRRTTSRN"
     gene            complement(2882..4186)
                     /locus_tag="VN478_02355"
     CDS             complement(2882..4186)
                     /locus_tag="VN478_02355"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HWQ21554.1"
                     /translation="MEKKLAIVGLGYVGLPLALTYALDGFSVAGVDIDPRRIESIRAS
                     SLGMREDFDGIPVNDVLRTCLANGSLNVTSKFGELPKDVTTFIITVGIPIDKTWALDM
                     GMLTGACTELGKLLKKGDLVICRSTVPVGTTRGLVLSTLEHESSLKAGRDFDLAYSSE
                     RIAEGRAYDEFRNMPLVVGGIDAKSLERAAEVLGAVNREPVFKASSIELVEAAKMFEN
                     AQRDVNIAVADQLARFANHFGIPTWELVEACNTHSRVKILMPGIGVGGHCIPYASPYL
                     FGSLSDQEECAKLLPTFVSARKANSGQPAYIAARMVASMGGLAGKHILFVGIAMKDYS
                     DDVTVSPAVDLAAALVEAGAEVHVLDTIAEAPIGLQRETDVDAGSSWADAIVLPIRQS
                     HIDYGRLKTLVAEAAGRLVLFDFRGVFEHDNEVLDQPWYVRL"
     gene            complement(4201..5349)
                     /locus_tag="VN478_02360"
     CDS             complement(4201..5349)
                     /locus_tag="VN478_02360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWQ21555.1"
                     /translation="MKALVLSTNNYRSFPSRKQRFAGRLVERGYDVLYVEAPVTWLAA
                     ARPKQWRKLVAGLSGTHKQAEGLWTVSPTPWLPYFKKYESIADHDSLTYLKYLRGLAG
                     DWTHDVGIVLSYLPFVPTTLQLLGAPVVYDCVDDHSAYPGLVDKATVDRLEARTADIA
                     SAVIATNESIAAKFSTHEDKLSLIQNGVDHELFAAPARAWLDTLTTLPREHKMLYAGA
                     MREWFDVQTLGMVAAAYPEWSLDCFGDALPAVRRQLSAHANILFKGTLSQAAIAQLAP
                     QYDVALIPFTESPLTVGVDPLKFYEYVAAGLPVVSSSIDALGVFGEDMVRLASTPGEF
                     IAAIRDSFDTDSLDLRRHRVESSRRFDWSFRIAEFDRVLDAMLTQDES"
     gene            complement(5346..>5677)
                     /locus_tag="VN478_02365"
     CDS             complement(5346..>5677)
                     /locus_tag="VN478_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21556.1"
                     /translation="LGSGLGTFGGSAAQKYGYFTGISMDSVWIRVLAETGLVGFLLYV
                     SWLASGFACVATRFFRSRDKLWLFASVGLLALLVNLFTDNLLDSWAIALLMWSLFALG
                     AIPEADE"
BASE COUNT         1237 a         1730 c         1676 g         1034 t
ORIGIN      
        1 ttccctgtac tggacgccaa agcgcgggac tggcacgaag atcggccgat cgcccagtgc
       61 cagcatccca gccagacgca ccagcatcac tgcgagctgt gaggccaacc ggtactcacc
      121 ctggaccttc atctggagga tagcctgccg caatgtccct tcgtaccagt acccggagta
      181 gccagatgcg gcacccgcag ccagtcgcga gtcacaccac ccagcgtcgc actggcgaaa
      241 tgacgaggca cagccggtgc agatagggag atccgattcc tctccgcaga gaaagcaggc
      301 tttgtcggcg agcgcctcat gaaggactga caagaaggtc acggggtcta gcgttccagg
      361 agcagtctca gtctggcaac gcggtcaatc ctggcctgcc tgaaccgcag gatggccgct
      421 tcggtcactg gcgacacggt cccgccaaga acccagcggg cagcttgctg gggaccattg
      481 cccgggggaa gagcgttgaa accgcaacag tccgccagct gggagacctt tgggtcgtag
      541 gcaagggcaa cgaggggaga ccgcgcggcg actgctgctg cggccatgtg atagcgccca
      601 gcaacagtca agctggctga gccacagagc tcaagcagtg ccagcggctc aggcgagatg
      661 atgagttcgg acggcattag cagacggttc ctgactgctc gcgtgaaccc caggtcggcc
      721 gacggaaaga gcacaaccag gtccagctga gcgcctgcct gagatccgag gcagtcaagg
      781 aaagacgcgc tctcctcggg cgtccagcca aaacggctgg taacacaggc aacaatgcgg
      841 ggttggcgac ggtctgggac gacatgttcc ggccgcaggg caatcaggtg cgacagcccg
      901 gcgtccgatg agacactgat ctcctgggct cggatcccca aacggagcag gagttcacgg
      961 gaggcatcgt cgcgaacatc aatgagactc atgcgatcga acacggtcct tacgagacga
     1021 cggatccatg gcctcctgac ggggcctatc ccttgtgcgt aggagatgac tgccttcctg
     1081 tgcagcagag ccagcagcgg aatgcccaga tagtacatgc tgcttcgcca gctggtcacg
     1141 tcctggacga gccctcctcc acccagcatc acgtggtcgc acgcagcgag tgctgctctc
     1201 atcgctgcca tgtcgctgcg atcgacgaac gagtgccgag ggcacgtggc cccggcgtac
     1261 tgacctggat ccttgacggc gaagaccgct tcgcactgaa cgccggacag ttcatcctgc
     1321 atggcggcga ggataagctc gtcgccgaag ttgccgaagc cgtagtagcc gaggacgagg
     1381 actcggttca tctagttcct cgaagtcgtc cgacgatgtg caagtacacg gaacatgaag
     1441 tagaagatgc tccccacgcg ccgcacgcgg cttggttgca ttacgagtcg gtacagccac
     1501 tcagttccag tcttctgaac cagccgcgga gccctgcgga cgagtccgct ccagacatcg
     1561 aaggtgccac caacgccgat ggcaacgggc acgccaatgt cgcggtgacg ccagatccac
     1621 ttctcctgcg ctcctccgcc catgccgacc aggagaatgt caacatgcgc tgcgcggatc
     1681 tcacggatca cgtctgcctc ctgggcgtcg tcgaagtagc ccgaatgaga gcccgcaacc
     1741 tgaagacccg ggtagcgagc agacatgttg gagctggcgc gctggacgat ctccggcttg
     1801 gcaccaagga agtacacgcg gtagcccctg cgctcagcca gggcaagcag agaactcgag
     1861 aactcaattc ccggtatgcg gttctctatc cgcgatccaa gtatgcgtgc accccagacg
     1921 atgccaacgc catcggcgac tacaagatct gcctgccgga caatctcgag gaattcggtg
     1981 tctctgaaag cacgagcagc catctcgcca ttgagcgtaa cgacgaggtg aggcacagca
     2041 ctcttcacaa actcgtcggc gatggcgagc agctcgtcca tgtccctgtt gctgatcgaa
     2101 atgccgaaga aatccatgct gtcgacatgt ctgcggggtg accggatcac aatgctcaca
     2161 aatcgcggaa gcgaaaggat gcgaggcaac cgcttcggat caagaacaaa gcggtacagc
     2221 cattcgagac cgagtctctg aacgcgcgca ggtgctcgtt tgaactctcc tgacaacacg
     2281 ttgtagctcc ctccaacgcc catggcaaag ggaacgccca tagccgcaag gttggccgca
     2341 aggaacttct cctgcttggg actccccatg ccgacgaaca tgagatctgg tttgagagcg
     2401 gcgatccctg caacaagacc gggctcgtcc tcttcagcga agtatccgtc gcgtgaccct
     2461 acgaccgtga gtctggggta cctctccctg acgatcgcca caaccctcga gagtacttca
     2521 gggcgtgatc cgaggaggaa cagcctccac tcccgggcgt cggccagatc aagcaatcgc
     2581 aggaacaggt cgactccggt gacccgggac ttgatgcgtt cgtggtagaa cagcctcgcc
     2641 gcccacatga ctccaatgcc atcgcagaaa ttcaggtcac tctttcgcag caccttctcg
     2701 agcgcgctgt cctgtttggc cttgatgatc ttctcggggt tgatcgcgac gcccatgtgc
     2761 ggggcgcgag acacgacgaa gccctccaca gctgcaaggg cttcgtccat gtccagattg
     2821 tcgacgggga tgccggcgac gtggactcgc tcagtcattc gcaggagagc cgatctggac
     2881 tctacagacg gacataccag ggttggtcga ggacctcgtt gtcatgttcg aacactcccc
     2941 tgaagtcaaa cagcaccaaa cggccggcgg cttcggcaac cagcgtcttg agccgcccat
     3001 aatcgatgtg gctctgacgg ataggcaaaa caatcgcatc ggcccacgac gagccagcgt
     3061 cgacgtctgt ttcgcgctgg agcccgatcg gcgcttccgc aatggtgtcc aggacatgca
     3121 cttctgcgcc cgcctcgacc agggccgctg ccaagtcgac agcgggactc accgtaacgt
     3181 cgtccgagta gtccttcatc gcaatcccga cgaacagaat gtgcttgccg gcgagcccac
     3241 ccatgctcgc aaccattcgt gcagcgatgt aggcaggttg accggagttt gccttgcgtg
     3301 cggacacgaa cgtcggaagc agctttgcgc attcttcctg gtctgagagc gagccaaaca
     3361 gatagggcga ggcataggga atgcagtgtc ccccgacgcc gatcccaggc atcaggattt
     3421 tgactctcga gtgtgtgttg catgcttcaa ccagttccca cgtgggaata ccgaagtgat
     3481 tcgcaaaacg ggccagctgg tctgcgacgg cgatgttcac atcccgctga gcattctcaa
     3541 acatctttgc cgcttctacg agttcgatcg aggatgcctt gaagacaggt tcgcggttga
     3601 ccgctccgag cacctcggcg gcacgctcga ggctcttcgc atcgataccc ccgacgacca
     3661 gcggcatgtt gcgaaactca tcgtatgcgc gtccttcggc tatccgctcg gacgagtagg
     3721 ctaggtcaaa gtccctgccg gctttcaggc tgctttcgtg ctcaagggtg gaaagcacaa
     3781 gcccccgtgt cgttcccaca ggtacggtag accggcagat gacgagatcc cctttcttca
     3841 ggagcttgcc aagctccgtg caggcgcccg taagcatgcc catgtcaagg gcccaagtct
     3901 tgtcgatcgg aatgccaacc gtgatgatga aggtcgtcac atctttcggg agctcaccga
     3961 acttgctggt gacattcagg ctgccgttgg caaggcatgt tcgaaggacg tcgttgaccg
     4021 gtatgccgtc gaagtcctcc ctcatgccaa gcgacgatgc tctgatcgac tcgattcggc
     4081 gcgggtcgat gtctacgcca gcgacgctga agccgtcaag cgcataggtc agcgcgaggg
     4141 gcagacccac atatccgaga ccgacaattg cgagcttctt ttccatctga tcctccatgt
     4201 ttaggactcg tcttgcgtca gcattgcatc gagcacccgg tcgaactcgg ctatacgaaa
     4261 cgaccagtca aaacgtctgg atgactccac acgatgacgg cgcaggtcaa gcgagtcggt
     4321 gtcgaagctg tcccgtattg cagcgatgaa ctctccggga gtggacgcca gacgcaccat
     4381 gtcttcgccg aacaccccaa gcgcgtcgat gctggaggag acaacgggca gcccagctgc
     4441 gacgtattcg tagaatttca gcgggtctac gcccaccgta aggggtgatt cggtgaaagg
     4501 gatgagcgcg acatcgtact ggggagccag ttgcgcgatc gcagcctggg acagggtccc
     4561 cttgaagagg atattggcgt gagcactcag ctggcgacgg acagcaggca gagcgtcacc
     4621 aaaacagtcg agtgaccatt ccgggtaggc cgcagccacc ataccgagtg tctgtacgtc
     4681 gaaccattcg cgcattgccc ctgcgtaaag catcttgtgc tctctcggca gcgtggtcaa
     4741 cgtgtcgagc caagccctcg caggcgcggc gaacagctca tggtccacac cgttctggat
     4801 caggctgagc ttgtcctcat gggtggagaa cttggcagca atactctcat tcgtcgcgat
     4861 cacagccgaa gcgatgtctg cggtccgggc ctcgaggcga tcgacggtag ccttgtcgac
     4921 cagaccggga taggcactgt ggtcgtcgac gcagtcatag accacggggg cgccaagcaa
     4981 ctgaagggtc gttgggacaa atggcagata ggagagaacg atgccgacgt cgtgggtcca
     5041 gtccccggca agaccccgca ggtacttcag gtacgtgagt gaatcgtgat ctgctatgct
     5101 ctcgtacttc ttgaagtacg gaagccacgg cgtcggcgat acggtccaca agccttctgc
     5161 ttgcttgtgt gtaccggaga gtcctgcaac cagcttccgc cattgctttg gacgtgccgc
     5221 tgccagccaa gtcacaggag cttccacgta gagtacatcg tagcctcgct ccacgaggcg
     5281 gccggcaaag cgctgttttc ggctggggaa cgatcggtag ttgttggtgc tgagaaccag
     5341 tgctttcatt cgtcagcctc cggtatggcg cctagcgcga agaggctcca catgagcaga
     5401 gcaattgccc aggagtccag gagattgtct gtgaacaggt tgaccagcag cgccaggaga
     5461 cccacagagg caaacagcca gagcttgtcc ctcgaccgaa agaaacgtgt ggcgacacat
     5521 gcaaaaccgc tcgccaacca cgagacatac agcaagaagc ccaccaggcc cgtttcggcg
     5581 aggacgcgaa tccatacgga gtccatcgag atacccgtga agtagccgta tttctgtgcc
     5641 gcagaacccc cgaacgtgcc aagaccacta ccaagaa
//
LOCUS       DATMZD010000112         2183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_113729,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000112 DATMZD010000000
VERSION     DATMZD010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113729"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="VN478_02370"
     CDS             complement(<1..902)
                     /locus_tag="VN478_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21557.1"
                     /translation="MSLNSARRRTAARAFLLLVAYYPLANWAIRKANIPLGNLWEEIV
                     LLIFLTFALVTSWRKVGRLLSSPVILSALLFLSVSLFGFAANTYYIGAYVQEVRLAFE
                     PFMAFAILWLLVDGDTSGLLREMVPHLVTSATIIAFIGVYQYVRKVPVPAQWLDKDTE
                     SGLISTRAFSLFGSPNVLAGYLEMIIPLGVYLTLARTKWYERCAVAVCVLVMGSGFLL
                     TLTRASWLSAAGSFFVGLLVLSPALAAGFAAAGAGILIAVPTFRLRMTNLLSTTYVDK
                     SNNLGRLFRWRQAFVNLSDHPLLG"
     gene            complement(910..1368)
                     /locus_tag="VN478_02375"
     CDS             complement(910..1368)
                     /locus_tag="VN478_02375"
                     /inference="COORDINATES: protein motif:HMM:NF025582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4330 family protein"
                     /protein_id="HWQ21558.1"
                     /translation="MKNKWIVGIVLGLVVIAGVAAAYKLTRPSTGAKGLSGSRIEATF
                     LAYEIQPATAAQIAAGSTIYDQTGKACFTITNVTTAPAQEVGIDSDGVLHSTAHPVLK
                     DVVITADSIGPKVAWAYMYGTDKILAGANVAIYGDLWKVWTRILTVHDLP"
     gene            complement(1365..>2183)
                     /locus_tag="VN478_02380"
     CDS             complement(1365..>2183)
                     /locus_tag="VN478_02380"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWQ21559.1"
                     /translation="DNGSAARYVPPEAYRQAVDVVRLERNIGPAGGAAAGQQRALSLG
                     ADWVWMLDDDVLVDPQALNRLLQDVEMHDSRTYFRSVCYNEPEPGLPFFNSFTYSRRT
                     GLLKPVARERYGDCRFRFDACAMAGLLLPSSLLQEVGVFDASLFGWYDDTEFTLRATL
                     AGFHGYAVPASRLLHPSANRRHLRLLGRSLAVLIDQPSRLYYGTRNCILTQRRLLARV
                     RFAALFLPFFAVRRFISIVLLYNNRRAFLRYFVMGVSDGLRGRKGELVQGGRTS"
BASE COUNT          506 a          625 c          648 g          404 t
ORIGIN      
        1 ctaccaagaa gtggatgatc actgagattc acaaaggcct gtctccagcg gaaaagacgc
       61 cccaggttgt tgctcttgtc gacatatgtc gtgctgagca ggttggtcat gcggagccgg
      121 aacgtcggaa cggcaatgag aattcccgcg ccggcggcgg caaaaccagc cgcaagagcc
      181 gggctcagaa ccaggagtcc gacgaagaat gatcccgcgg ccgacaacca ggaggcccgc
      241 gtcagcgtga gcaggaaccc gctgcccatg acgaggacgc acacggccac ggcgcaccgt
      301 tcataccact tcgtccgcgc tagggtcagg tacacaccca ggggaatgat catctccagg
      361 taccctgcga gaacattggg gctgccaaac agcgagaagg cgcgtgtgct gatgaggcca
      421 ctctcagtat ccttgtccag ccactgagcc ggtaccggca ccttccgcac gtactggtac
      481 acacctatga atgcgatgat cgtggcgcta gtcacaaggt gcggaaccat ctcgcggagg
      541 agacccgacg tgtcaccgtc gacaaggagc cacaggatgg cgaacgccat gaacggttca
      601 aacgcgagcc ggacctcctg gacatatgcg cctatgtagt atgtattggc agcaaagcca
      661 aacagagaga ctgaaagaaa gagaagtgca gaaaggatga cgggtgaact cagcaagcgt
      721 ccgacctttc tccagctcgt gacgagcgcg aatgtgagga agatcagaag cacgatctct
      781 tcccagaggt ttccaagcgg gatattcgcc tttcggatgg cccagttggc cagaggataa
      841 tatgcgacga ggaggaggaa agctcttgct gcagtacgtc tcctcgcgga gttgaggctc
      901 atcctgtctc tatgggagat cgtgcactgt gagaatgcgc gtccagacct tccacaagtc
      961 gccgtagatg gcgacgtttg ccccagccag aatcttgtcg gtaccgtaca tgtaggccca
     1021 cgcgactttc ggaccgatgg agtctgctgt gatgaccaca tctttgagaa ccgggtgtgc
     1081 agtcgagtgc aggacaccgt cgctgtcaat gccaacttcc tgggccggcg cggttgtgac
     1141 gttcgtgatg gtgaaacagg ctttgcctgt ctgatcgtat atggtagagc ccgctgcaat
     1201 ctgtgcggcc gttgccggct ggatctcata ggcgaggaat gtagcctcga tccgactgcc
     1261 gctgagaccc tttgctcccg tggacggacg cgtgagctta tatgctgcgg cgaccccggc
     1321 gatgaccacg agccctagga cgatcccgac aatccacttg ttcttcatga cgtacgaccc
     1381 ccttgtacaa gttcgccctt tcggccacgg aggccgtctg agacccccat cacgaagtat
     1441 cgcagaaagg cacgacgatt attgtagaga agcacaatcg aaatgaaacg gcgaactgcg
     1501 aagaacggaa ggaacagagc cgcaaaccgc actcgcgcga gcagcctgcg ctgtgtgaga
     1561 atacagttgc gagttccgta gtacagtcgc gaaggctggt cgatcaggac ggcgagggac
     1621 ctgccaagaa gccgcaagtg tctccggtta gctgagggat gcagcagtct gctcgccgga
     1681 acggcatatc cgtggaatcc cgccaacgtc gctctgagtg tgaattctgt atcgtcatac
     1741 cagccgaaca gggacgcatc gaagaccccg acttcctgca gcagggatga cggaagaagg
     1801 aggcctgcca ttgcgcatgc atcaaagcga aatcggcaat cgccataccg ttcgcgcgcc
     1861 accggcttga ggagtcccgt ccgacgactg tatgtgaacg agttgaagaa cggaagtccc
     1921 ggttctggct cattgtagca cacggaacgg aagtacgtcc tgctatcgtg catttcgacg
     1981 tcttgcagca agcggttcag agcctgcgga tcgacgagaa cgtcgtcatc aagcatccat
     2041 acccagtcag caccaaggct cagggccctc tgttggcctg ccgccgcgcc tcctgccggc
     2101 ccgatgttgc gttccagtcg aacgacgtca acagcctgtc ggtatgcctc gggaggcacg
     2161 tacctggcag cagacccgtt atc
//
LOCUS       DATMZD010000113         3265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_143903,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000113 DATMZD010000000
VERSION     DATMZD010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143903"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /locus_tag="VN478_02385"
     CDS             complement(<1..781)
                     /locus_tag="VN478_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012996225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HWQ21560.1"
                     /translation="MTNGEIVKVAGPLVVARGLTNPQMYELVRVGDAKLFGEIIELRG
                     ELASIQVYEETSGVGPGEPVVQTGSLLTVELGPGLITSIYDGIQRPLDAIRVQYGDFI
                     PRGVALPALPRERKWHFVPCMKVSDTAIEGDIIGTVQETKAVLHQIMIPKGLAGKIVS
                     ILEGDFTVEDPVAVIQTDSGEQRQVSMIQRWPVRNSRPFTRRMAPDAPLVTGQRVIDA
                     LFPVAKGGTACVPGPFGSGKTVIQHQLAKWANADIIVYVGCG"
     gene            complement(1109..1942)
                     /locus_tag="VN478_02390"
     CDS             complement(1109..1942)
                     /locus_tag="VN478_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21561.1"
                     /translation="MARVESTWQAFLIQAARDLPESFLPYLLKALDEKEYAKAGLAEV
                     LLGENGGRRGDRPSGEYAAYCTRIQAEGGTPEVLSESVFGWALSEGIKAAQAGASASD
                     AQTVFDFSFQATLSDLARAYGHPAVMAYVDGLLKLMLAGHALKMKLRSTSGVGAPLER
                     LLPFLDLTVRAELASVAERIEGISWDTLQPVDVVLEASAYVLPVGRRYTPEERLQMLE
                     DALEAWLVGRPEATGIEPYGVGVIFAYLMDFRREVWSLRRLLKAAASARPHVEGSRGA
                     S"
     gene            complement(2143..2721)
                     /locus_tag="VN478_02395"
     CDS             complement(2143..2721)
                     /locus_tag="VN478_02395"
                     /inference="COORDINATES: protein motif:HMM:NF014093.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033178 - proton-transporting two-sector
                     ATPase complex, catalytic domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E family protein"
                     /protein_id="HWQ21562.1"
                     /translation="MGTEELRAAVLQEATAEAASIIEAATAEASQLVDSQRRQLEKHN
                     GTLVAERRASRDHDIVTQTAALKIELRNAILKRKQELLEGVYQELEQKVRGDTVLYHA
                     YLERAVERVGAEAPISIECRSVDESVVRGLLLKRADWSGVRIDASLPDTDGGFVAHFA
                     EAELDLTLSGASVAMREDTLVETAQALFGDTK"
     gene            complement(2734..>3265)
                     /locus_tag="VN478_02400"
     CDS             complement(2734..>3265)
                     /locus_tag="VN478_02400"
                     /inference="COORDINATES: protein motif:HMM:NF012365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21563.1"
                     /translation="VTAVGGSFAFKLSGTSMASLGSVWVFAGAALVIVFGGLGSAIGI
                     RISGSQAAGVLSEKPELFGKMFVMMALPGTQGFYSFVCGIMIIVLSGVLNKGAAISNA
                     KGLALLTVGVSVGLVEWLSAILQGQASAAAESLTGRQPSTSGQAILIPALVETYAVIA
                     LVSGILLILWISQAAF"
BASE COUNT          678 a         1075 c          877 g          635 t
ORIGIN      
        1 ctccacagcc cacatacact atgatgtctg catttgccca ttttgcgagc tgatgctgga
       61 tgactgtctt gccgctgccg aacggaccgg gaacacatgc cgttccgccc ttggcaacag
      121 gaaacagagc gtcaatgacg cgttgcccgg tcaccaacgg ggcatccggc gccatgcgac
      181 gtgtgaaagg cctgctgttg cgtaccggcc aacgctggat catggacacc tgacgttgct
      241 cgcccgagtc agtctggatg accgctacgg gatcttcgac ggtgaaatcg ccctccaata
      301 tcgagacgat ctttcccgcc agacccttgg ggatcatgat ctggtgcagg acggccttcg
      361 tctcctggac agtcccgatg atgtctcctt ctatcgctgt gtcactgacc ttcatgcacg
      421 ggacgaagtg ccacttccgt tcacgaggga gggcaggtag agctactccg cggggaatga
      481 agtcaccgta ttgcacacgg atcgcgtcaa gcggtcgctg gatcccatca tagatcgacg
      541 tgatgagacc aggtccgagc tcaacggtaa ggaggctccc cgtctgaaca acaggttcgc
      601 ccggcccaac cccgcttgtc tcttcataga cctggatgct cgccaattcg cctcgaagct
      661 cgatgatctc accaaagagc ttcgcgtcgc cgacgcgtac cagttcatac atctgcgggt
      721 tcgtcagccc cctggcgact acgagcggtc cggcaacctt gacgatctca ccgtttgtca
      781 tggcttttct cctccgtcat tttcccgact aggtcaattc ctacagcact ctctatgaga
      841 cgccttacgg cagcagtcgc tgtccccgta tctgatgagg tcaccggcac cacaagcacg
      901 acgggtacca ccagcctacg tgctgcggcc gactcggacc atacatcgcc tacatcggac
      961 gtcagcaaga tgagtccaaa ttcccctgaa cggacaatcg cctggatcgc ctgggacgcc
     1021 tgcagaccgt cctcgaccac cgtgccagca aaacccagcg cctcgaaagc cccgactgag
     1081 tcgcgcgtcg tcacaaccgc tgctctgctc acgaggctcc tctggaccct tcaacgtgcg
     1141 gtctggcgga cgctgccgct ttcagaagtc gtcggaggga ccacacttct cgccggaagt
     1201 ccatcaagta ggcaaagatg acccccactc cgtacggttc gatccccgtc gcctcagggc
     1261 gtccaacaag ccatgcctcc agtgcatcct caagcatctg cagccgttct tcgggcgtgt
     1321 accgccgccc gactggaagc acgtacgcag aggcttccag aacaacgtcc acgggttgga
     1381 gagtgtccca cgatatgccc tcgatacgtt ccgcgaccga cgccagctcg gcccgtactg
     1441 tcaaatcgag gaatggcagc aacctctcga gaggggcgcc gacgccggat gtactgcgaa
     1501 gcttcatctt cagtgcatgt cccgcgagca tcagcttcag caggccatcc acataagcca
     1561 tcaccgccgg gtgcccatat gccctcgcca gatcgctaag cgtagcctgg aaagagaagt
     1621 caaaaacggt ctgggcgtca gaagccgaag cccccgcttg cgccgccttg atcccctccg
     1681 agagcgccca gccaaaaact gattcggaca gtacttcggg ggtgcctccc tcggcctgga
     1741 tacgcgtaca ataagctgcg tactcaccgc ttggtcgatc accccgtctt ccgccgttct
     1801 cgccaagaag gacttcggcc agtccagcct ttgcatattc cttctcgtca agggctttga
     1861 gcaagtacgg aagaaaactc tctggcaggt cacgggcagc ctgtatcagg aaggcctgcc
     1921 aagtgctctc aactctggcc aaggcctctc ctgccgacat ctccgccccg acgggcgcat
     1981 aggctccacg gttgagcgca gtcagcagag gctcgatccc gcctgtctgc gatgtacgcc
     2041 gcagctcctg aactggcagg agcaatcctc gcctgacaga gaaggagcca gacgcgtatg
     2101 catagctgct cgaatcagct tggtgcggag tgaacggagc aatcatttcg tatcgccgaa
     2161 cagggcctgt gctgtctcga caagcgtatc ctctcgcatg gcgacggacg cccctgaaag
     2221 ggtcaggtcg agttccgcct ccgcgaagtg agcaacgaac cctccgtcgg tgtcggggag
     2281 cgacgcgtcg atgcgtaccc cggaccaatc cgcacgtttg aggagcagcc ccctaaccac
     2341 ggattcgtcc acgctgcggc actcgattga tatcggtgcc tccgctccaa cccgctccac
     2401 cgctcgctcg agataggcat ggtaaagaac agtgtcaccc ctcaccttct gttcgagttc
     2461 ctggtagacc ccttcaagca gttcctgttt gcgcttgagg atagcgtttc tgagctcaat
     2521 cttcagcgca gctgtctgtg tcacaatgtc atgatcccgg ctagcgcgac gttctgctac
     2581 cagcgtgccg ttgtgcttct ccagctgtcg cctctggcta tcgaccagtt gcgaagcctc
     2641 agcagtggcg gcctcgatga tgctggccgc ctctgcagtc gcttcctgga gaaccgcagc
     2701 cctgagttcc tcggttccca tgtgcacgac gtcctagaag gctgcctgag agatccagag
     2761 gataagcagg atacccgaga ccaacgcgat gacggcgtat gtttcgacga gggcggggat
     2821 aagaatggcc tgccccgacg tcgacggctg gcgaccggtc aggctctctg cagcggccga
     2881 agcctgtccc tggaggattg ctgagagcca ttcgacgaga ccgacactca caccgactgt
     2941 caagagagcc agccccttgg cattggaaat cgctgctccc ttgttcagaa cccccgacag
     3001 aacgatgatc atgatgccac acacgaagct gtagaagccc tgtgtccctg gaagcgccat
     3061 catgacgaac atcttgccga acaactcagg tttctccgac agcacgccgg ccgcttgaga
     3121 acccgatatg cggatgccta tcgccgagcc aagcccgcca aatactatca caagcgcggc
     3181 tcccgcaaac acccatacag agccgagcga tgccatcgat gtgccggaca gcttgaaggc
     3241 aaacgaccca cctaccgctg taacc
//
LOCUS       DATMZD010000114         2565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_73289,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000114 DATMZD010000000
VERSION     DATMZD010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73289"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..893
                     /locus_tag="VN478_02405"
     CDS             <1..893
                     /locus_tag="VN478_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HWQ21564.1"
                     /translation="KTRLGNADLKGPIEAAVEEEVELYLDKHIDEFRAMANKAVSAKL
                     AREAARKARDLVRRKTELDGGRLPGKLADCSSQDPAESELYIVEGDSAGGSAKQGRNR
                     RTQAILPLRGKILNVEKVGIHRALENEEIKSLITCVGTGVMDDIKIEKLRYHKIVIMT
                     DADVDGAHIRTLLLTFFYRYMRPLVVNRNIYFAQPPLFSFADGKTTRYAFSDDELRTL
                     LSQVKGKPDVQRYKGLGEMNPEQLWSTTMDPATRLMGRVTLEDAEEAERIFTLLMGEK
                     VEPRRKFIVDHAREVYNLDI"
     gene            911..>2565
                     /locus_tag="VN478_02410"
     CDS             911..>2565
                     /locus_tag="VN478_02410"
                     /inference="COORDINATES: protein motif:HMM:NF012731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HWQ21565.1"
                     /translation="MPENDRVFNVYVEEEMKKSYLDYSMSVIIGRALPDFRDGLKPVH
                     RRILYAMYELGCFWNKPYKKSARIVGDVLGKYHPHGDTAIYDSLVRMAQEFSLRYPLV
                     DGHGNFGSIDGDSAAAMRYTEVRLAPVSALMLDELDKEIVEFTPNFDNSLEEPSILPA
                     KVPQLLINGSSGIAVGMATNIPPHNLGELIEALKYMLDCRIGGEPEDPGQVRSFVRGP
                     DFPGGGLVVGTEGIDEYISTGRGNIVTRGKYTIEKDGNKARFVITELPFEVNKAEFVQ
                     KVADLMKDKHLAQLSDLRDESSREGIRVVLDLKKDAIPERVELLLRKHTQFQQHFHVS
                     MLGILYGVPRTLPLRDVLATFLDFREATLRKRFAQELRKHEERLNVLNGLVIAIRNVK
                     RVTDILTSSSSMDDASARLVQAFGLNDLQVEAILEMRMRSLVSLEVEKLAKEVEENRT
                     EIERLSAMLADPNRIRIYIRDELAEVARKYGNPRRTQILKDDGRGEDDDEQFIEDKEV
                     MVVISKNGYAKIIPSSTFKVQGRGGLGVASMNLKRDDYISKYFT"
BASE COUNT          584 a          684 c          768 g          529 t
ORIGIN      
        1 cgaagacgcg gctcgggaat gccgatctga agggccccat cgaggctgct gtcgaggaag
       61 aggtcgaact gtacctcgac aagcacatcg acgagttcag ggctatggcc aacaaggctg
      121 tcagcgcgaa gcttgcgcgc gaggctgcac gcaaggcgcg tgaccttgtc cgccgcaaga
      181 cggagttgga tggtggacgc ttgccaggca agctggcgga ctgttcttct caggacccgg
      241 cggaatcaga actctacatc gtcgaaggcg attcggcagg tggtagcgcc aagcagggcc
      301 ggaaccggcg cacgcaggca atcctgcccc tgcgaggcaa gatcctgaac gttgagaagg
      361 tcggcattca tcgggccctt gagaatgagg agatcaagtc tctcatcaca tgcgttggta
      421 caggggtcat ggacgacatc aagatcgaga agcttcggta ccacaagatt gtcatcatga
      481 cggatgctga tgtcgatgga gcgcacatcc ggacgctgct cctgactttc ttttaccgct
      541 atatgcgacc tctcgttgtc aaccgcaaca tctacttcgc tcagcccccc ctcttctctt
      601 tcgctgatgg caagacgacg cggtatgcgt tctccgacga cgagcttcgg acactgctgt
      661 cgcaggtgaa gggcaagccg gatgtccaga ggtacaaagg actgggagag atgaaccccg
      721 agcagttgtg gtcaacgact atggacccgg caactcgcct tatggggcgt gtcacgctgg
      781 aagacgccga ggaggctgaa cgcatcttca cgctgctcat gggtgagaag gtggagcctc
      841 gtcgcaagtt cattgtcgat catgcgcgtg aagtctacaa cttggacatt tgacggagcg
      901 agggacagtg atgccagaaa acgatagagt gttcaacgtc tacgttgagg aggagatgaa
      961 gaagtcctac ctcgactatt ccatgagtgt catcatcggc agagcgctgc cggacttccg
     1021 ggacggcttg aagcctgtgc atcgccgcat cctctatgcg atgtacgagt tggggtgttt
     1081 ctggaacaag ccgtacaaga agagcgcccg tatcgttggt gacgttctgg gcaagtatca
     1141 cccacacggc gatactgcca tctacgactc gcttgttcgc atggcgcagg agttctcgct
     1201 caggtatcca ctggtggatg gtcacggcaa ttttggatcc attgacggtg attctgctgc
     1261 agccatgcgt tatacggagg ttcggctggc tcctgtctct gcactcatgc tggatgagct
     1321 ggacaaggag atcgttgagt tcactccgaa cttcgacaac agtctggaag agccgagcat
     1381 cctgccggcc aaagtaccgc agcttctgat caacggttcc agtggcatcg ctgtgggcat
     1441 ggccaccaac atcccgccgc acaacctggg ggaactcata gaagccctga aatacatgct
     1501 ggactgccgt attggcggcg aaccagaaga tcccggccag gtacggtcat tcgtgagggg
     1561 tcccgacttt cccggtggcg gtctggtagt tggcacggag ggcatcgacg agtacatatc
     1621 taccggtcgg ggcaacattg tcaccagagg gaaatacacg atcgagaagg atggcaacaa
     1681 ggcgcgtttt gtcatcacag agctgccgtt tgaggtgaac aaggctgagt tcgtccagaa
     1741 ggttgccgac ttgatgaagg acaagcatct ggcccagctt tcggatttgc gcgacgaatc
     1801 cagccgcgag ggcatacggg tcgtgctcga cctgaagaag gacgcgatac ctgagcgagt
     1861 cgagctattg ctgcgcaaac acactcagtt ccagcagcac tttcatgtca gcatgctcgg
     1921 catcttgtat ggtgttcccc gtacactgcc tctgcgtgac gtgctggcga ccttcctcga
     1981 tttccgcgag gcaactctcc gcaagaggtt cgctcaggaa ttgcgcaagc acgaggagcg
     2041 cctgaacgtt ctcaacggcc tcgtcatcgc cattcgcaat gtcaagcggg tgacagacat
     2101 tctgacctcg tcctcgtcaa tggacgatgc gtcggctcgc cttgtccagg cctttggact
     2161 caatgacctg caggtcgaag cgatcctcga gatgcgcatg cggtctctcg tgtcgcttga
     2221 ggtcgagaag cttgcgaaag aggtcgaaga gaacaggacg gagatcgaga ggctctccgc
     2281 gatgctggca gaccccaaca ggattcgtat ctacatcagg gatgagcttg cagaggtcgc
     2341 tcgcaaatat ggaaacccac gccgtacaca gatcctgaag gacgatggtc gtggagaaga
     2401 cgatgacgag cagttcattg aagacaagga agtcatggtt gtcatctcca agaatggcta
     2461 cgcgaagatc atcccgagtt cgacattcaa agtccagggt cgtggtggac tcggcgtcgc
     2521 gagcatgaac ctgaagcgtg acgactatat cagcaagtac ttcac
//
LOCUS       DATMZD010000115         3967 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_43375,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000115 DATMZD010000000
VERSION     DATMZD010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3967
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43375"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..813)
                     /locus_tag="VN478_02415"
     CDS             complement(<1..813)
                     /locus_tag="VN478_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21566.1"
                     /translation="MENVRGKLTYRRDGVVTTLDFTLGGQVENDDLTISPAISAVVPG
                     ERWTVTVTPRTAVTLLAAELRIPLPVPHGSHVFVNGYQTWTESRELGTRDRIPPLNRL
                     ARSRCAMYGDYEFVTNPGFPGRFHGWTYGYVRTGIDLLTLFGSLADHTGFTLVTVTAS
                     SGRVTLQKECGGVVPTGPYLLYDVYAGTGTDREAFGRYFDLLGLQPLQTPPLTGWTSW
                     YNYYTSVAEEIIQHNLDALSAQQVPLDVFQIDDGWEHAIGDWLTTNHKFPAGR"
     gene            complement(815..1984)
                     /locus_tag="VN478_02420"
     CDS             complement(815..1984)
                     /locus_tag="VN478_02420"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HWQ21567.1"
                     /translation="MSNQVLLQDFERVYGTSDSRIRVFFAPGRVNLIGELIDYNGGLV
                     MPAAIALGITAVCRARSDRIARLRSSDDSLPVEIDLDQPIEPDPSRGWGNYPAGVLRE
                     LLHQGSDLVGADVLFTTTLPQGAGLSSSAAMEVLTAFCFLALAELPLSDLKGMALLCQ
                     KAENQFVGVNCGIMDQFAVALCRQDHALLLDCDSQDFTHIPVAMGEYALVIMNTSRQR
                     RLAESKYNERRAECEAVLRLLGTDAPHNGLVHAQWSDVLSHVADPILQRRARHVITEH
                     KRVQEASRVLTTGDLVAFGKLLNESHTSLRDDYEVTGSYLDAIVSAAQQAPGCLGARM
                     TGAGFAGCAIALVRQADVSAFTAFVRASYAAMTGIDPSFYETRAVDGVHETTGDI"
     gene            complement(1981..2970)
                     /gene="galT"
                     /locus_tag="VN478_02425"
     CDS             complement(1981..2970)
                     /gene="galT"
                     /locus_tag="VN478_02425"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009353392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HWQ21568.1"
                     /translation="MAELRWNPLLRTWTMVAGNRQARPNLRSDGCPFCPGSGNVPDSY
                     DVLEYDNDFPALNMDPDQPDAVGSDLSPVAPNYGKCEVILYSPQHDASLHDLSVAHLT
                     RLVELWTERQRVLSGDPRIKYIFPFENRGEEVGVTMRHPHGQLYAYPFVPLKIRTELD
                     SARAWYREHGSCLFCDLTDNELSDGRRVILSNESFVAYVPSFTDYPYGVSVTSRAHKG
                     SFLDFTPSEQGDLAWMLKTLTGGFDVLFDRRFPYMMCIHQTPVNSPEDEGCEQFYHFH
                     IEFYPPLRSENTVKYYASSEMGAWAACNPLRVEDTAPVLAAACRAFERTAVQS"
     gene            3136..>3967
                     /gene="galE"
                     /locus_tag="VN478_02430"
     CDS             3136..>3967
                     /gene="galE"
                     /locus_tag="VN478_02430"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009144922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HWQ21569.1"
                     /translation="MTTGTVLVTGGAGYIGSHTVRRLVRNGYQVAVFDNLSKGHRWAV
                     PRDVPIIVGDVADTAYVAMTMRQFHVTSVIHFAAFSLVGESMIEPRAYYANNVIGTLR
                     LLDAMRTADVDRIVFSSSAAVYGEPAMVPIEEDSALAPTNVYGRTKMTIEGILHDYGV
                     AYGLRSVSLRYFNAAGADPDGGIGEDHDPETHLIPLVLQAAAGRRPSVSVFGNDYPTV
                     DGTCVRDYIHVNDLADAHILALESLERQTEAVYNLGNGDGFSVRQIIETTQRIVGHDI
                     P"
BASE COUNT          784 a         1128 c         1238 g          817 t
ORIGIN      
        1 cctcccggcc gggaacttgt gattggtcgt cagccagtcg ccgatggcat gttcccaccc
       61 gtcgtcgatc tggaagacat cgagagggac ctgttgtgcg ctcagggcgt caaggttgtg
      121 ctggatgatc tcttccgcta cgctcgtgta gtagttgtac caacttgtcc agccagtcag
      181 aggcggcgtc tggagaggtt gaagccccag gaggtcgaag tagcggccaa atgcctcgcg
      241 gtcggttccg gtccccgcgt agacgtcgta cagaaggtag ggaccggtag gcacgacacc
      301 gccgcattcc ttctggagcg tgactcttcc tgatgaggcg gtgaccgtca ccagggtgaa
      361 ccccgtgtgg tcggccagcg agccgaacag agtgagcaga tcaatccctg tacgcacgta
      421 tccataggtc cacccgtgaa agcgtcccgg aaagccagga ttcgtcacaa actcgtagtc
      481 gccgtacatc gcgcaccgcg agcgggccag ccgattgagc ggagggatgc ggtctctggt
      541 acccagctcg cgtgactcgg tccatgtctg gtagccattc acgaagacat ggcttccatg
      601 tggcacagga agcgggatcc gcagttccgc tgcgagcagc gttacggcag tacgaggcgt
      661 cacagtgact gtccagcgtt ctccagggac tactgcgctg atcgcgggcg aaatggtcag
      721 gtcgtcgttc tcgacctgtc cgcccagggt gaagtccagg gtcgtgacga ccccgtcgcg
      781 acgataggtc agtttgcctc gtacgttctc cattctatat gtctccggtt gtctcgtgga
      841 ctccatcgac tgcccgggtt tcatagaacg aaggatcaat tcccgtcatc gccgcatagg
      901 aggctcgaac aaacgcggtg aacgccgaca cgtcggcctg ccgtacgaga gcaatcgcgc
      961 agcccgcgaa accggcaccc gtcatgcggg cccccaggca ccctggagcc tgttgcgcag
     1021 cgctcacgat cgcatcgaga tatgatccgg ttacttcgta gtcgtcgcga agtgacgtgt
     1081 gagactcgtt gagcagcttc ccgaatgcca ccaggtctcc ggttgtcagc acacgcgatg
     1141 cttcttgcac gcgcttgtgc tctgtgatga catggcgcgc ccgtcgctgc agtatgggat
     1201 ccgctacgtg ggacagtacg tcgctccatt gagcgtggac aaggccattg tgaggtgcgt
     1261 ctgtcccaag gagccggaga acggcctcgc actcggctct gcgttcgttg tactttgact
     1321 cggccagtct tcgctgtctc gaggtgttca tgatgaccag cgcatattct cccatagcga
     1381 cgggtatgtg cgtgaagtcc tgcgagtcgc agtccagcag aagggcgtgg tcctgtctgc
     1441 aaagcgcgac ggcaaactgg tccatgatac cgcagttgac ccctacgaac tgattctctg
     1501 ctttctgaca cagcaaggcc atgcccttca ggtcactcag ggggagttcg gcaagcgcga
     1561 ggaagcagaa tgctgtcagt acctccattg cggctgacga cgagagccct gctccttgtg
     1621 ggagtgtcgt ggtgaagagg acgtcggctc cgacgaggtc acttccctgg tggaggagtt
     1681 cgcggagtac tcctgccggg tagttgcccc agccgcgcga agggtcgggt tcgatgggtt
     1741 ggtcgaggtc aatctcgaca ggtagtgagt cgtccgatga ccgcaatctg gcaatacggt
     1801 cggagcgagc ccggcagact gcggttatgc cgagcgcgat tgctgccggc ataaccaggc
     1861 cgccgttgta gtcgatgagc tctccgatca ggttcaccct gccgggagcg aagaagacac
     1921 ggatcctgct gtccgaggtg ccgtacacgc gctcgaagtc ctggagaagt acctgattgc
     1981 tcacgactgc accgccgttc gttcgaatgc cctgcatgcc gccgcaagaa ccggggcagt
     2041 gtcctcaaca cggagaggat tgcatgccgc ccatgcgccc atctccgatg acgcgtaata
     2101 cttcacggtg ttctcgctgc ggagaggagg atagaactcg atgtggaagt ggtagaactg
     2161 ctcgcaccct tcatcttcgg ggctgttcac aggcgtctgg tggatgcaca tcatgtaggg
     2221 aaaacgacgg tcgaacagca cgtcgaaccc tcccgtgagg gtcttcagca tccaggcaag
     2281 gtccccctgt tccgatggcg tgaagtcgag gaacgatccc ttgtgtgcac ggcttgtgac
     2341 ggagacgcca tatgggtagt ccgtgaatga cgggacgtag gcgacgaagc tctcgttcga
     2401 caggatgacg cgcctgccgt cggacaactc gttgtcagtg aggtcgcaga agaggcaact
     2461 tccatgttcg cggtaccagg cgcgagcgct gtccagttcc gtgcggatct tcagcgggac
     2521 aaatggatag gcgtagagct gtccatgggg gtgtcgcatg gtgacgccaa cctcctcgcc
     2581 acggttttcg aatgggaaga tgtactttat ccgagggtcg cccgagagga ctcgctgtct
     2641 ctcggtccag agttcaacaa gacgcgtcag atgtgcgacg ctcaagtcgt gcaggcttgc
     2701 atcgtgctgc ggagagtaca ggattacctc gcactttcca tagttggggg cgaccggcga
     2761 caggtccgac ccgactgcgt cgggttggtc gggatccatg ttcagtgccg ggaagtcgtt
     2821 gtcgtactcc aggacatcat agctgtcagg gacattgccc gatccagggc aaaaagggca
     2881 gccatcactg cgcaggtttg ggcgtgcctg ccggttgccc gcaaccatag tccatgtccg
     2941 caagagtgga ttccatcgta gttccgccat cgtgatcctc cagtgtgctg cagcgccggg
     3001 gcgccagctt cacgacatgc agtatagtcg aacggatccg ttggtacagg tcggatcacg
     3061 ctgtcagtct gttcaggcgg tctgtgcgga ctatactgat tgagcaacca actacatgtc
     3121 gaagaggaga aggcaatgac gactggaacg gttctcgtga ctggtggtgc cggctacatt
     3181 gggtctcaca ccgtacggag gctggtgcgg aacggctatc aagtcgccgt gttcgacaac
     3241 ctttcaaagg gacaccgctg ggcggtgcca agggatgtcc caatcatcgt cggagacgtc
     3301 gcagacacgg cctatgtggc catgaccatg cggcagttcc atgtgacgag cgtcatccat
     3361 ttcgcggcct tcagccttgt cggtgagtcg atgatcgagc ctcgcgccta ctacgcgaat
     3421 aacgtgatcg gtacgctgcg tcttctcgac gccatgcgta cagcggacgt cgaccgcatc
     3481 gtcttctcca gctcggcagc cgtctatgga gagccggcga tggttccaat agaagaggac
     3541 agcgccctgg cgccgaccaa cgtctacggg cgcaccaaga tgactatcga gggaatcctc
     3601 catgactatg gcgtagcgta tgggttgcgt tcagtgtcgc tgaggtactt caacgcggct
     3661 ggagccgatc ccgatggtgg gatcggcgag gaccatgacc cggaaacgca tctgatacct
     3721 ctggtactgc aggctgcagc cggtcggaga ccgtctgtga gtgtgtttgg caacgactac
     3781 ccgacagtgg atggcacctg tgtccgagac tacatccacg tgaatgatct tgcagatgct
     3841 cacatccttg ccctggaatc gctcgagcgg cagacagagg cggtctataa tctgggaaac
     3901 ggagatggat tctcggtcag acagatcatc gagaccaccc aacggatcgt agggcatgac
     3961 atacccg
//
LOCUS       DATMZD010000116         2009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_134536,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000116 DATMZD010000000
VERSION     DATMZD010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134536"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="VN478_02435"
     CDS             complement(<1..444)
                     /locus_tag="VN478_02435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HWQ21570.1"
                     /translation="MRIATWNVNSVRARLPVIQKWLQESLVDVLAMQETKTVDETFPV
                     AEFEALGYQAAVSGQKTYNGVAIVSRLPVSGVVKGWAGNDPSRILAVDVGGLTLVDGY
                     LPHGGERGDEAFLHKLEFFQRFRTYLDATYLPDRHLLFMGDFNVAM"
     gene            complement(448..1050)
                     /locus_tag="VN478_02440"
     CDS             complement(448..1050)
                     /locus_tag="VN478_02440"
                     /inference="COORDINATES: protein motif:HMM:NF016362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HWQ21571.1"
                     /translation="MPDIQGLWSIVRQRVAEWAGRIVAAGWDAFLPFVPSLVATAVFL
                     ALIVAVGIAVRIHRDHVFHGATLRSIDAMDGTQFEEFLAVLFTHLGYRVQHVGMSHDF
                     GADLVLTSTRCRIVVQAKRYAGNVGIGAVQEALGAVHYYRGTRGMVVASSGFTESARE
                     LAARSGIELWDRQRLAAVMARTVGRGRTVGRKPAYNVHEE"
     gene            complement(1043..1825)
                     /locus_tag="VN478_02445"
     CDS             complement(1043..1825)
                     /locus_tag="VN478_02445"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HWQ21572.1"
                     /translation="METRKEQRLGLALSSGGVLGYAHIGVLRALEELHVQVDLLSGAS
                     MGAIVAVFNAAGLDAAGLEALSVKYSAQVAMLFGAIVPVNRLTTPSRLRASLRKDLPV
                     TVFEALQKPVCIAATDVLTGNALVFDSGDIWSPLSGSFGLPGVFPVVKVGDQYVLDGC
                     FSLPVPVTELRKRGATSVIAVSVYDVAQHEDFKPSWNIPKVLELSVNLTFAKIARPEL
                     DAADLVIRPDLKGCEPSDVHEYALRGYEATMARQAEIEALLA"
BASE COUNT          411 a          674 c          575 g          349 t
ORIGIN      
        1 catggctaca ttgaaatcgc ccatgaacag cagatgacgg tccggcaagt acgttgcgtc
       61 gagatacgtc cggaacctct ggaagaactc cagcttgtgg aggaacgcct cgtcaccacg
      121 ttcgccaccg tgcggcagat acccgtcgac cagcgtcagg ccaccaacgt ccaccgccag
      181 gatccggctc gggtcattgc ctgcccagcc cttgacgaca ccggagacag gcagtctcga
      241 cacgatggcg acgccgttgt acgtcttctg gccggacact gccgcctggt atcccagcgc
      301 ttcgaactcc gcgacaggga acgtctcgtc caccgtcttg gtttcctgca tggcgagcac
      361 gtccaccagt gactcctgca gccacttctg gatgacaggc agacgagccc gcaccgagtt
      421 cacattccat gtcgcgattc tcatgggtca ctcctcatgg acattgtatg caggtttccg
      481 cccaaccgtg cggcccctgc ccaccgtcct tgccatgacg gccgcgagac gctgtctgtc
      541 ccacaactcg atccccgacc tggccgccag ttcccgagcc gattccgtaa acccgctgct
      601 cgcgacgacc atgcctcgcg tgccacggta gtaatgcacg gcccccagag cctcctgcac
      661 ggcgccgatg ccgacgtttc cggcgtacct cttcgcctga acgacgatcc ggcaacgagt
      721 cgacgtcaga acaaggtcgg ccccgaaatc gtgactcatg ccgacatgct ggacgcgata
      781 gcccagatgt gtgaacagca cggccaggaa ctcctcgaac tgtgtcccat ccatggcgtc
      841 gatcgagcga agcgtagccc cgtgaaacac gtggtcacga tggatccgga cggcaatgcc
      901 cactgcgacg atcagggcga ggaaaacggc agtcgccacc agagaaggca caaacggcaa
      961 gaatgcatcc cagccggctg ccacaattct gccggcccat tcggcgacgc gttgccgcac
     1021 tatgctccag agtccctgga tgtcaggcaa gcagggcctc gatctccgcc tgcctggcca
     1081 tggtggcctc gtaccctcgc agggcatact cgtggacgtc tgagggctcg caccccttga
     1141 ggtcaggacg gatgacaaga tccgccgcat caagttccgg cctggcaatc ttggcgaacg
     1201 tgaggttgac tgacagctcg agcaccttgg gtatgttcca gctgggcttg aagtcttcgt
     1261 gctgagcgac gtcatagaca gacacggcga tgacgcttgt tgcgccgcgc ttccgcagct
     1321 cggtcacagg gacaggcagt gagaagcagc catccagcac atactgatcg ccgaccttga
     1381 cgactggaaa cacgcctggc aagccgaagc tccccgacaa cggagaccag atgtccccgc
     1441 tgtcgaaaac aagggcgttg ccggtaagta cgtcggtcgc ggcaatgcac acaggcttct
     1501 gcagagcttc gaagaccgtg accgggaggt ctttgcgaag ggaggcccgc agacgcgagg
     1561 gggtcgtcag acgattcacg gggacgatgg ccccgaagag catggccacc tgtgcactgt
     1621 acttgacgct cagggcttcc aggccagccg cgtcaagtcc cgccgcgttg aaaacggcca
     1681 cgatggcacc catcgaggca cccgacagga ggtcgacctg gacgtgaagc tcttccagcg
     1741 cccgcaacac gcctatgtgc gcatagccaa ggacgccgcc cgaactgagg gccagcccca
     1801 gtctctgctc cttgcgagtc tccacggtca cgcctcctgt tccggggtcc tgagtgaccc
     1861 ctccccagca gtatattgcg gatgtggcgg ccgggcaaca gcgctgccca ccataccttc
     1921 cggcttgaca cgaccccctc catcctatac ttggaatgac atcaggaggc gcacatggcc
     1981 attttcgacg tcatgggacc gatcatgat
//
LOCUS       DATMZD010000117         5125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_23252,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000117 DATMZD010000000
VERSION     DATMZD010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5125
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23252"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VN478_02450"
     CDS             <1..367
                     /locus_tag="VN478_02450"
                     /inference="COORDINATES: protein motif:HMM:NF013768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HrcA family transcriptional regulator"
                     /protein_id="HWQ21573.1"
                     /translation="EGELRRGESQIFVRGIEQMLSELPDDQIGAFSTLSTFLSREEVV
                     KPYLNSLSNRGEISLFIGEENQREELRSFSLVSVSYSLDSNQRGVLGVVGPIRMNYVR
                     AITVLESFAGYLGRVSGSG"
     gene            371..1096
                     /locus_tag="VN478_02455"
     CDS             371..1096
                     /locus_tag="VN478_02455"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HWQ21574.1"
                     /translation="MPAFEAGRSLKLGEHVMLGGSPAAHHIVDVLRARHGERIVLFHA
                     GQLYDAVVEDSSRSGLSVSVEGVRQSPFLGHSAVLLQAAIRPTLLDDVVHVCAPLGVS
                     RFVLYAAEYSQPWNVAGRLERLNQVAMSSAEQAETGNVPAVELETALQGALASLEPGG
                     TLIMMSPRATSTMTQCARDGSLALDGPVVVAVGPEGGFSGSEEALLADRGALSVRLNS
                     GILRSELAGFAAMLVLRELQATR"
     gene            1093..2532
                     /locus_tag="VN478_02460"
     CDS             1093..2532
                     /locus_tag="VN478_02460"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWQ21575.1"
                     /translation="MKVFIYTLGCKVNEVDAELYAQELRFLGAETTTNLVDADAIIVN
                     SCSVTNRAQAQSLQFARRAVRERPGAAVYLTGCGSALLSLTHAAAPAGVSVVAMRDRS
                     EVARLVVGSGDAARPAGRWVDGCIQMVGPRNRVDVRVQEGCDNMCTYCVVPFVRGSRL
                     ESKAVDVAVDEVLGLARLGVKEVVLTGTEIGKYGQAPLRGAARTGGTHSGSVPSASDQ
                     RAQGALGSHARGEAPLALEEHARGESQDRLAGLLENMLVALEDSGLPTRVRVSSLNPD
                     AISERLVSQFAVHARLCPQLHLPLQSGSDAILQRMRRPYLSRDVLDSVLRLRAVDPLF
                     ALTTDVIVGFPGETEQDFRATLDVIRLAAFAKVHAFPYSPRPFTPAAREDHQVPAGIA
                     KERVHRVLAVAEATGITFARRFIGRDVTIVSEKTSPGEVEGYSEHYLWTEGHLFSRRV
                     VDHDALVVVHVENCRFDGDRVVLSGSVPA"
     gene            2529..2894
                     /locus_tag="VN478_02465"
     CDS             2529..2894
                     /locus_tag="VN478_02465"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HWQ21576.1"
                     /translation="MRDACERDCIFCNIGRHAAPAEYVYEDETVFVIKDRFPKAPVHL
                     LVIPRKHVTRIDALDAEDDTLIVHLFEVIRLIAAKYHLEEGYRVIINSGAGSGQTVPH
                     LHVHIVAGKCLGSREDVAL"
     gene            complement(2891..3391)
                     /locus_tag="VN478_02470"
     CDS             complement(2891..3391)
                     /locus_tag="VN478_02470"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HWQ21577.1"
                     /translation="MSQQGMVALLKDALQLEIEGWVFYRDVTGNLPEGAVRDVFIYLA
                     EQEEKHQRYIGTELERVRGGGILDISGLAALAETTHDEVLADAVRSARDLSQHEASAI
                     HTGMLLERNSWEFYHTAAAKAIEPDEVSMYGQLEEWEKLHLHLLEKAYDLLKERIWAE
                     NRFAPF"
     gene            complement(3388..>5125)
                     /locus_tag="VN478_02475"
     CDS             complement(3388..>5125)
                     /locus_tag="VN478_02475"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Zn-binding domain-containing protein"
                     /protein_id="HWQ21578.1"
                     /translation="YHGVLGSHMALLLRRLRRLANFYGAHPVFFVMSATIDEPGTFAS
                     QLTGEFVQDVDSSGAPKGRRTLLLFNPKIVDKQNNIRKSVMKETLWLFQRAVQEDVRT
                     IVFTKSRQEAELLYKYVRDAGAGKGQRLGDRVATYRAGYLPAVRRGIEHRLSSGELTG
                     VIATNALELGIDIGHLDVSITAGYPGSVTSLFQQMGRAGRERDHSLSIFVASSNPLDQ
                     YVVQTPGFLSSGHFGTPTINPDNEYILSAHLRCAAYELPLRAATDVEYFGPQYAELVE
                     QLEADGKLRPRGPLVVSTETYPHRFVSLRTLSDKRYHVLNDGDGEVLEEADAYKVIEE
                     FYPGAVFLHQGDSYLVTSDDHEQQVVHATPTTQNYYTDAIIRSDVVVQNERSRHDYGD
                     VSLSFGKVVVSEQTVGFIKKSFHIETEVGREYIDTPEISYDTMALWITFPDAMLDVLK
                     EGGYDIAGSLHAAEHLLIALMPIVVLCDRRDLGGISTILHRDTLKPSIFVYDGHIGGV
                     GLTESAYGRFRDVVQLALDRVTVCPCKDGCPSCVYSPKCGNNNKPLDKNGAHVLLGLL
                     LDRLETTPGGGK"
BASE COUNT          951 a         1470 c         1643 g         1061 t
ORIGIN      
        1 ggaaggagaa ctgcgcaggg gagagagcca gatctttgtc cgcggcatcg aacagatgct
       61 gagcgagctg ccggacgatc agattggagc cttctctacg ctgagcacct tcctcagccg
      121 cgaagaagta gtgaagccat acctgaactc gctgagcaat cgcggcgaga tcagcctgtt
      181 catcggcgag gagaaccagc gcgaggaact gaggagtttc agtctcgtgt cggtgagcta
      241 ctcgcttgat tcgaatcagc ggggcgtcct cggtgtcgtg ggccccatcc gtatgaacta
      301 tgtccgggcc atcacggtcc tggagagctt cgcggggtat ctcggcaggg tctcgggcag
      361 cggctgatcc atgccggcat tcgaagcggg tcggtcgctc aagctgggcg aacacgtcat
      421 gctgggtggt agtcctgctg cgcaccacat tgtggatgtc ctccgcgctc gccatggtga
      481 gcggatcgtt ctgtttcacg ctgggcaact gtatgatgca gttgttgagg actcgtccag
      541 atcaggtctg tccgtctcag tggaaggtgt ccgtcaatcg ccctttctcg gtcactcggc
      601 cgtgctcctg caggcggcga tccgcccaac gttgctggac gacgtggtac atgtctgcgc
      661 cccgctgggt gtgagccgct tcgtgttgta tgccgccgag tacagccagc cgtggaacgt
      721 cgctggacga ctcgaacgcc tgaaccaggt ggccatgtca tctgcagaac aggctgagac
      781 ggggaacgtc cctgcggtgg aactggagac agcactccag ggcgccttgg catctctgga
      841 gcctggcgga acgctgatca tgatgtctcc gcgggcgacc tcgacgatga cgcagtgcgc
      901 tcgcgacggg tcacttgccc tggatggccc ggtcgtggtt gctgtcggcc ccgagggtgg
      961 gttctcggga tcagaggagg ctctgctcgc ggacagaggc gccctgtctg tccgcctcaa
     1021 ctcgggcata ctcaggagtg agctggccgg attcgcagcc atgcttgtgc tgcgggagct
     1081 gcaggcgaca cgatgaaggt gttcatctac acactggggt gcaaggtgaa cgaagtggat
     1141 gctgaactct atgcacagga gctgcgattc cttggtgccg agacaacaac gaacctggtg
     1201 gacgccgatg ccatcatcgt caactcgtgc agcgtcacga accgggctca ggcccagagt
     1261 ctgcagtttg cccgccgcgc ggtgcgcgaa cgacccggtg ccgccgtcta cctgacagga
     1321 tgcgggtcgg cattgttgag cctcacgcat gctgcagccc ctgcaggcgt cagtgtcgtt
     1381 gccatgcgtg atcggtcgga agtggcgaga ctcgtggtcg gatcgggtga cgccgcgcgg
     1441 ccagctgggc ggtgggttga cggatgcatc cagatggttg gtccacgcaa ccgcgtcgat
     1501 gtgcgtgttc aggagggctg tgacaacatg tgcacctact gtgtggtgcc gttcgtacgt
     1561 ggctcgcgcc tggagtccaa ggccgtcgac gtggctgtag atgaagtcct tgggctggcg
     1621 agactgggtg tgaaggaggt cgtgctgacc gggacagaga tagggaaata cggacaggca
     1681 ccgctcagag gcgccgctcg tacgggagga acacactcgg ggagtgtgcc ctctgcatcg
     1741 gaccaacgtg ctcaaggggc attgggcagt catgctcgcg gggaagcgcc actcgcattg
     1801 gaagaacatg ctcgcgggga gtcgcaggac aggctggctg gactcctgga gaacatgctt
     1861 gtggcgctgg aggactcagg ccttccgaca agggtgcgtg tcagttcact gaaccctgat
     1921 gcgatctccg aaaggctggt cagccagttc gcagtacacg cacgactttg tccgcaactg
     1981 catcttccgc tgcagagcgg cagtgacgcc atactgcagc gcatgcgtcg accatacctg
     2041 tcgagggacg ttctggacag cgtcctgcgc ctgagggcgg tcgacccact gtttgccctg
     2101 acgaccgatg tcatcgtcgg ttttcccggc gagaccgagc aggactttcg ggccacgctg
     2161 gatgtgatcc gcctggcggc gttcgccaag gttcacgcct tcccctattc gccgcgtccg
     2221 ttcacgcctg ctgcgcggga ggatcatcag gtcccggcgg gaattgccaa ggagcgcgtc
     2281 caccgcgtgc tcgctgtcgc agaggcaacg ggcatcacat tcgcccgccg gttcatcggt
     2341 cgggacgtaa ccattgtgtc cgagaagacc tctccagggg aggtcgaagg atactcggag
     2401 cattacctct ggacagaggg acacctgttc agcaggaggg tggtcgacca cgacgccctg
     2461 gtcgtcgttc atgtcgagaa ttgccgtttt gacggtgacc gggttgtcct ctccgggagt
     2521 gtgcctgcat gagggatgcc tgcgaacggg actgcatctt ctgcaacatc ggtcggcacg
     2581 cagctccggc cgaatacgtc tatgaggatg agacagtctt cgttatcaag gaccggtttc
     2641 cgaaggcgcc ggtccacctg ctggtcatcc cgcgcaagca cgtcacccgc atcgatgctc
     2701 tcgatgctga agacgacacg ttgatcgtcc acctcttcga ggtgatccgg cttatcgcgg
     2761 ccaagtatca cctggaggaa ggataccgcg tcatcatcaa ctcaggcgct gggagtggac
     2821 agaccgtgcc gcacctgcac gttcacatcg tagcggggaa gtgtctgggg tcgcgggagg
     2881 acgttgctct ctagaacggc gcgaaacgat tctccgccca gatgcgttct ttgaggaggt
     2941 catatgcctt ctcgagaaga tggaggtgga gcttctccca ctcctccaat tgcccgtaca
     3001 tgctgacctc gtctggctcg atggccttcg cagccgcagt atggtagaac tcccatgagt
     3061 tgcgttccag cagcatgccc gtgtggatcg cggatgcctc gtgctgcgaa agatccctgg
     3121 cgctgcgtac tgcatcggcg aggacctcgt cgtgggtcgt ctctgccagt gccgcgagtc
     3181 cactgatgtc cagtatgccg ccccctcgta cgcgctccag ctctgtaccg atgtaccgct
     3241 ggtgcttctc ctcctgctca gcaagataga tgaagacgtc gcgtacagcc ccttcgggca
     3301 ggttgccggt tacgtcgcgg tagaacaccc atccttcgat ctccagctgc agtgcatcct
     3361 tgagaagagc aaccattcct tgttgactca cttgccacct cccggggtcg tttcgagtct
     3421 gtcgagcaga agcccgagca gaacatgtgc gccgttcttg tctagcggct tgttgttgtt
     3481 gccgcacttg ggtgagtaca cacagctggg acagccatcc ttgcaggggc acactgtcac
     3541 gcggtccagc gccagttgaa cgacatcgcg gaatcgtcca tacgctgatt cggtgaggcc
     3601 aacgccaccg atgtgcccat catagacgaa aatgctcggc ttcagggtat cccggtgaag
     3661 tatcgtggag atacctccaa ggtcacgccg atcgcatagc acgacgatcg gcatcaaggc
     3721 gatgagcagg tgctcggcgg cgtgcaggct ccctgcgata tcatagccgc cttccttcag
     3781 gacatcgagc atggcgtcag ggaaggtgat ccagagggcc atcgtgtcat agctgatctc
     3841 cggcgtgtcg atatactcac gcccgacctc tgtctcgatg tgaaagctct tcttgatgaa
     3901 gccgacagtc tgctcggaca cgacgacctt gccgaaggac agactgacgt cgccatagtc
     3961 atggcggctc ctttcgttct gaacgacgac atcgctgcga atgatcgcgt cggtgtagta
     4021 gttctgcgtg gtgggggtgg cgtggacgac ctgttgttcg tggtcgtcgc tcgtgacgag
     4081 atatgagtct ccctggtgga ggaatacggc gccagggtag aactcctcga tgaccttgta
     4141 cgcgtctgct tcttcgagga cctctccatc gccatcgttc aggacgtgat agcgcttgtc
     4201 ggatagtgtc cgcaggctga cgaagcggtg aggatatgtc tcagtcgaca cgacaagggg
     4261 accgcgtggg cgcagcttgc cgtctgcctc cagctgctcg acgagttccg catattgcgg
     4321 accgaagtat tccacgtcgg ttgcggcgcg cagtgggagt tcgtacgcgg cgcagcggag
     4381 atgagcgctc aggatgtact cgttgtcagg gttgatcgtc ggcgtgccaa agtggcctga
     4441 cgacaggaag cccggggtct gtacgacgta ttgatcgagc gggttggaag aagcaacgaa
     4501 gatggagagc gagtgatcgc gttcgcgtcc cgcccgcccc atctgttgaa agaggctggt
     4561 cactgaacca gggtagcctg ccgtgataga tacgtcgaga tgcccgatat cgattcccaa
     4621 ctcgagggcg ttggtcgcta tgacaccggt cagctcaccc gacgacagac ggtgttcgat
     4681 gcctcggcga actgcaggga ggtagcctgc tcggtaggtt gcgacacggt ccccaaggcg
     4741 ctgacccttg cctgctcctg catctctgac gtacttgtac agcagctccg cttcctggcg
     4801 cgatttagtg aagacgatcg tccgcacgtc ttcctggacg gcacgctgaa agagccacag
     4861 tgtctccttc atgacgctct tgcggatgtt gttctgcttg tcgacgatct ttgggttgaa
     4921 caggagcaga gtacgccggc ccttgggggc tcccgacgaa tcaacgtcct gaacgaactc
     4981 tccagtgagc tgggacgcga atgttccggg ttcatcaatg gtagcggaca tgacgaagaa
     5041 caccgggtgt gcgccataga aattggccag ccgccgcaga cgccgcagga ggagagccat
     5101 atgcgagcca agtacgccat gatag
//
LOCUS       DATMZD010000118         2381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_144102,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000118 DATMZD010000000
VERSION     DATMZD010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144102"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VN478_02480"
     CDS             <1..219
                     /locus_tag="VN478_02480"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HWQ21579.1"
                     /translation="ADEFARRVAVEAPACTGAISQLATLFGKARYSRPSVTGEDVLAA
                     KAALQDLRVCVRSMRSEARTSRRPARGR"
     gene            276..1226
                     /locus_tag="VN478_02485"
     CDS             276..1226
                     /locus_tag="VN478_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015283209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HWQ21580.1"
                     /translation="MNENLNKAIAYVEQYFVGKNTVVDVAFATLLAGGHIFLEDVPGL
                     GKTTLANLISRSLGLEFKRIQFTPDLLPSDISGVSVYDQKTQEFRLKRGPIFTNLLLA
                     DEINRGTPKTQSALLEAMAEYQVTIDGTIVKLERPFVVMATMNPIEYEGTFPLPEAQL
                     DRFMTRLTIGYPSKEKEEEIVNGFYARVKVISDDVRIISREELTAMQKDVEGVFIAPE
                     LITYIVSLVDATRHDDTVYLGLSPRGSIDLMRLSKAYAYLQNRDFVIPDDIKLVFPFI
                     SGHRLILQAEARLRGVTTNEVVTTTLKNVPIPKLADYSKQ"
     gene            1235..>2381
                     /locus_tag="VN478_02490"
     CDS             1235..>2381
                     /locus_tag="VN478_02490"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HWQ21581.1"
                     /translation="MHTASRGLALGAGLAVLWLIMGSHALFFTACAVILYSTMLPVAM
                     MRSIDKVGVSVALRRQLVMMGKPDMVSIALTGTEPYSPLRYGLSLSLPPYLIKKHERR
                     TYLALMVFFEANRRGLYQVGDTIVTVEDPLRVFSLSRVFPSSQRVTVFPRLLSFPRLR
                     IALTSPLDGQRVRYAPNVDTSQLSGTHPYDGEPMNRIHWKVSAHAGALQAKDFSPSAS
                     KTVVILLNLTLRHTPVFDRDVFEDYMCTVAGSIANYAGDAHLPFGLIVLGREQLTSGY
                     GTGSAHLVKCLSLLARAQSVIEPDSARAVFLDYLQTRRFAIPPRSQLFLVQQEMTENE
                     IGALLRMRAQFSRLSIALFPQGSFRHYGEHKTPYFMQDYVQIERLRSA"
BASE COUNT          485 a          775 c          647 g          474 t
ORIGIN      
        1 gccgatgagt tcgcacggcg cgtggcggtc gaggcccctg cctgcacagg agcgatcagt
       61 caactggcaa ccctctttgg aaaagcacga tacagccgcc cgagtgttac cggcgaagac
      121 gtcctcgcgg ccaaggccgc actgcaggac ctgcgcgtgt gcgtccgcag catgcgcagc
      181 gaagcccgga cctcacgacg accggcacga ggtcgttgat ttcgaaccga acagcctaca
      241 atggaaaaga cagcatacta cagaaggagc tgaccatgaa cgagaatctc aacaaagcca
      301 tagcctatgt ggaacagtac ttcgtcggca agaacacggt cgtcgacgtg gcttttgcga
      361 cgttgctcgc cggcggacac atcttcctcg aggatgtccc cggcctcggc aagacgacac
      421 tcgccaacct tatctctcgt tcactggggc tggagttcaa gcgtattcag ttcactcccg
      481 acctgttgcc atcggatatc tcgggcgtgt cggtctatga ccagaaaacg caggaattcc
      541 ggctgaagcg aggccccatc ttcactaacc ttctgctggc agacgagatc aaccgtggca
      601 cccccaagac acagtcagct cttcttgagg ccatggctga gtaccaggtc accatcgacg
      661 ggaccatcgt caagctggaa cggccgtttg tggtcatggc taccatgaac cccatcgagt
      721 atgagggaac cttccctctg cccgaggcac agctggaccg tttcatgacc cgcttgacga
      781 tcggctaccc cagcaaggag aaggaagagg agatcgtcaa tggcttctat gcccgcgtga
      841 aggtcatcag cgatgacgtc cgcatcatct cgcgtgagga actgaccgcc atgcagaaag
      901 acgtcgaggg agtcttcatc gcgcccgaac tcatcaccta catcgtctca ctggtcgacg
      961 ccacgcggca cgacgataca gtctatcttg gactgtctcc ccgcggcagc atcgatctca
     1021 tgcgcctcag caaggcatat gcatacctcc agaaccgtga cttcgtcatc ccggatgaca
     1081 tcaagctcgt cttcccattc atatcgggac accgactcat cctccaggct gaagcccgcc
     1141 tccgcggcgt gacgaccaac gaagttgtga ccaccactct caagaacgtt cccatcccca
     1201 agctggccga ctacagcaag caatgaggcg gtacttgcac accgcctcgc gcggccttgc
     1261 tctgggggcc gggctggctg tgctgtggct gattatgggc agccatgcac tgttcttcac
     1321 ggcgtgtgca gtgatcctct acagtactat gctacctgtc gccatgatgc gctcgatcga
     1381 caaggtcggc gtctcggtgg ccctcaggcg ccagctggtg atgatgggca agccggacat
     1441 ggtcagcatc gccttgaccg gcactgagcc gtacagccca ctccgctacg gcctttcgct
     1501 ctctctaccc ccatacctga tcaagaaaca cgaacggcgt acgtatctcg ccttgatggt
     1561 cttcttcgag gccaacaggc gcgggctgta tcaggtgggc gatacgatcg tgaccgtcga
     1621 ggatcccttg agggtgttct cgctgtctcg cgtgtttccg agctcgcagc gtgtcacggt
     1681 ctttccccgg ctgctgtctt ttccccggct gcgcatcgct ctcacatccc ctctcgacgg
     1741 acagcgtgtc cggtacgccc cgaatgtcga caccagccag ctctccggga ctcacccata
     1801 tgacggcgag ccgatgaacc gtatccactg gaaagtaagc gcgcatgccg gggcactcca
     1861 ggcgaaggac ttcagcccca gcgcttccaa gactgttgtc atcctcctca acttgacgct
     1921 gcgccacacg ccggtcttcg acagagacgt attcgaggac tacatgtgta ccgtcgcagg
     1981 ctcgatcgcc aattacgccg gcgacgccca cctgcccttc ggactgatag tgctgggacg
     2041 agagcaattg accagcggtt acggaaccgg gagcgcccat ctcgtcaagt gcctgtcgct
     2101 cctggcgcgc gcccagtccg tcatagagcc tgactctgcc agggccgtct tcctggatta
     2161 tctccagact cgcaggttcg ccatcccgcc acgttcgcag ctgtttctcg tccagcagga
     2221 aatgaccgag aacgagatcg gtgcacttct ccggatgcgt gcgcaattct ccaggctgtc
     2281 catcgccctg tttccacagg ggagcttccg acactacggc gagcacaaga ctccgtattt
     2341 catgcaggac tacgtacaga tcgagcgtct gcggtcggcc c
//
LOCUS       DATMZD010000119         7026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_83994,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000119 DATMZD010000000
VERSION     DATMZD010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7026
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83994"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="VN478_02495"
     CDS             <1..724
                     /locus_tag="VN478_02495"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWQ21582.1"
                     /translation="GSRTAYMEEPWPGTDGNHGYHTYESVDWLADRFRKADENGLWVW
                     IHAIGDRANKETLDAFEQAGVAASKGHAHHRIEHAQFLRPQDVERFADLGIAASVQPS
                     HLDLDIGKLKTVFPTPQPRSYAFRSLLDSGVELDFGSDAPVEDPDALKGIAFAAFRTR
                     AGELPYQSEQCISVQDALTAYTVAPQNSVGMSGQRGNLVEGQDADVVVLSRDILQDNE
                     PQSVRSTRVLATFVAGNVAFEA"
     gene            complement(798..1322)
                     /locus_tag="VN478_02500"
     CDS             complement(798..1322)
                     /locus_tag="VN478_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21583.1"
                     /translation="MKRFFVALMVIALTVLNTSPVAANAEQPPVLSTNDLVENSYKWD
                     GKTVSFKGEVLQDLMVRADGTWMNLSDGNNVAMGVFVPKSVMTPVISHTEGYRTIGDV
                     VLITGVFHRVCTEHEGETDIHATSVTVVTPGAARANPIHQSRVVWLLVLACLLGLVMW
                     FYYRKPTDEAKHPE"
     gene            complement(1319..2842)
                     /locus_tag="VN478_02505"
     CDS             complement(1319..2842)
                     /locus_tag="VN478_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013782401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HWQ21584.1"
                     /translation="MIRRVWREDFRIIGYYTGLVTIGVGLLMLVPMVTSLVFREWASA
                     LDFAISVVLTLLVGYVLALISRTRNTPGLTHAMVIAAGSWLIAMVLSGVPYMLSGHMK
                     SFLDACFDTMSGFTTTGLFLLQDLDHISQGLNMWRHLLTYVGGQGIVVLALTFLIGSS
                     GGAFKIMVGEGKEEKLEPNVRHIAILIWRISLIYLAVGTIVLGFIAIGNGIRPSLAFF
                     HGAYIFMSAWSTGGFAPYSQNMLYYHSSGMDIVSMVFFILGSFNFALHYTIWRGNRKE
                     ILRNVEIVSFTITVLTTFSLVCLGLMQRGVYPNAVALFRRGFFQLVSGHTTTGQSNIY
                     ARTFVREWGPLGMLATTIAMAIGASAASTGGGFKGLRMGIIVKGIVTDIKKLLSPERA
                     VVTGHYHHLHDNTLTDGVVKSAAVIVVLYVLIYVVGAVVGMAYGIPMADALFESVSAG
                     SNTGLSCGVTQATMPTLLKIIYILEMWMGRLEFLSVFTLFGFIATSFRSMRLRKVAE"
     gene            complement(2846..2983)
                     /locus_tag="VN478_02510"
     CDS             complement(2846..2983)
                     /locus_tag="VN478_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21585.1"
                     /translation="MTLGRRISRTTSRRNELLGMLTEVAFTCGLIGAGFLISWLFFVG
                     R"
     gene            complement(2989..3648)
                     /locus_tag="VN478_02515"
     CDS             complement(2989..3648)
                     /locus_tag="VN478_02515"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HWQ21586.1"
                     /translation="MYIIIAGGGKVGSYLAHLLVGKGHDVAVIEQDAGACEALSEWGK
                     ALVINGDCCDLSALIDAGIEKTDRFAAVTGDDANNLVACQLAKVRFNVPLTTARINDP
                     RNEDIFHKFGVDFALNSTNILATVIEEGIIVNDLIPLVSIAKGKATLVELKVADGSVA
                     AGKTLRELSWPRGCVVASVVHDGNVIFPRGATELNAGDVVVGLVAPEAEQQFRQVFHQ
                     A"
     gene            complement(3653..4309)
                     /locus_tag="VN478_02520"
     CDS             complement(3653..4309)
                     /locus_tag="VN478_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HWQ21587.1"
                     /translation="MYIIIVGCGRLGAQLATKLSEEGHDVAVVDRNSSNFARLSDEFN
                     GATITGVGFDIDVLKSAGIEHADGFAAVTDSDNMNIMAAQVAKTIFKVPMVIARVFDP
                     IKGETYRALGLETVCPTTSAANIIYSKFLIRNEESHFILPGGGIELVEVAFHTSIPAT
                     TIAGVEALDDFKVISLTRGGTTTFPKGNDVLAEGDQLLIALHVSDLPKVSSIFHLDER
                     "
     gene            4520..6373
                     /locus_tag="VN478_02525"
     CDS             4520..6373
                     /locus_tag="VN478_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWQ21588.1"
                     /translation="MQEPVENQVIKTSPRGSRPRSLRAASLGVWGQSGAVMAFYHSGA
                     LADIVIILGILGVFYGIAQVGREWTGILRPTVLIDLSFGALPRYTFFSLVRGLAAYCI
                     SFLFTIVYGFWAAKDKLAERLLVPILDILQSIPVLGFMPGVVLALVSLFPNSNVGLEL
                     AAVLMIFTGQAWNMTFSFYNSLKSVPQYLQEAGTIYRFNWWQRLTKIEMPYSAVGLIW
                     NSMMSMAGGWFFLMVTEAFQLGNRDFRLPGIGSYMSVAVEKGNVPAMAAAVVAMVLMI
                     VALDQLLWRPTVVWAQKFRIEDTSDEETMDSWFLDLLHHSHVLRWIGAAESSVRRAVG
                     TAISRVFRRTPREVPAGGLSAPAPDVHETPNRLSPAVVFSRALFLLLAVGLAFALSQI
                     VRLLVSVRIGEWLRLLRSDLLTLARVTAAIALGTLWALPVGLRIGLSPKLSRIMQPVV
                     QVAASFPAPMLFPAVILVLTLLHVNIGFGSIVLMLLGTQWYILFNVIAGAQAIPSDLK
                     EAAASYRVTGRQRFWSLYLPAIFPYLVTGWVTATGGAWNASIVAEIVTFRGQTLRAQG
                     IGSVISEAAFGAHFAELAAAILIMSLTVVLINRFVWRRLYAVASTQYNLSK"
     gene            6385..>7026
                     /locus_tag="VN478_02530"
     CDS             6385..>7026
                     /locus_tag="VN478_02530"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWQ21589.1"
                     /translation="MEDSVQQPILLSSENVTQRFPMPNGNMVTVLDNISMDIRKGEII
                     CLLGPSGCGKSTFLRILAGLMRPTSGKVFARGKEMAGLTPGVGMVFQMFALFPWMTVS
                     ENIQSVLVPLQLPREEVQNRVSTVIKMVGLEGFEEAYPREISGGMKQRVGIARGLAVQ
                     PELLFMDEPFSAVDALTAESLRTDLVDIWDEADGQPASIVMVSHDIKEVVYMAD"
BASE COUNT         1431 a         2165 c         2042 g         1388 t
ORIGIN      
        1 gggttcccgg acggcgtaca tggaagagcc atggcctgga acagacggca accacggcta
       61 ccacacctac gaatccgtcg attggctggc tgaccgattc cgcaaagctg acgagaacgg
      121 tctgtgggtg tggatccatg ccatcggtga cagggccaac aaggagacgc tggacgcctt
      181 cgaacaagct ggtgtggctg cttccaaggg acatgcgcac caccgcatag aacatgccca
      241 gttcctgcgc ccccaggatg tcgagcgctt cgccgacctg gggatcgcag cctcggtaca
      301 gccctcgcat ctcgacctcg acatcggcaa gctgaagacg gtcttcccca cgccacagcc
      361 tcgttcgtac gccttcaggt cgctcctcga cagcggcgtg gaactagact tcgggtcgga
      421 cgcccccgtc gaagacccgg atgccctcaa gggcatcgcg ttcgcggcat tccggacacg
      481 agccggcgaa ctcccctatc agagtgagca gtgcatcagc gtgcaggacg cacttacggc
      541 gtacactgtg gcgcctcaga actccgtagg catgtctgga cagcgtggca acctcgtcga
      601 gggacaggac gccgatgtcg tcgtactgtc gagagacatc ctgcaagaca acgaaccgca
      661 gtcagtacgt tccaccaggg tccttgcgac ctttgtggca ggcaacgtcg cattcgaagc
      721 ctgagtgatc cccaatacag agagcagaag gccccgcgca agcggggcct tctcaacgtg
      781 tatcggtctt ctgcaggcta ctccgggtgc tttgcctcgt cagtcggttt gcggtagtag
      841 aaccacatga ccagaccgag gagacaggca agaacaagga gccacacgac tctgctctga
      901 tgaatggggt ttgccctcgc tgcgccaggc gtgaccactg tgacactcgt cgcgtggata
      961 tcggtttccc cttcgtgttc ggtgcacact cggtggaaga caccagtgat aagcacgacg
     1021 tctccgatgg tacggtagcc ctcggtatgc gagatgacgg gcgtcataac actcttcggc
     1081 acgaatacgc ccatagcgac attgttgcca tccgacaggt tcatccatgt gccgtcggcg
     1141 cgcaccatca ggtcctgcag gacctctccc ttaaaggaca cagtcttgcc atcccacttg
     1201 taggagttct cgacgaggtc attggtcgag aggacgggag gctgctcggc gttcgcggca
     1261 accggagaag tgttcagaac ggtgagtgca ataaccatca gggcaacgaa gaacctcttc
     1321 attcggccac cttcctgagc ctcatgctcc ggaagctcgt ggcgatgaac ccgaacaggg
     1381 tgaacaccga caggaactcc aggcgtccca tccacatctc caggatgtag ataatcttga
     1441 gcagtgtcgg catggtcgcc tgcgtcaccc cgcacgacag cccggtattg ctgccggctg
     1501 acacggactc gaacagcgca tctgccatgg ggatcccgta cgccataccg acgaccgcac
     1561 ccacgacgta gatcagcaca tacagcacga cgatgacagc cgcactcttg accactccgt
     1621 ccgtgagtgt gttgtcgtga aggtggtgat agtgccccgt gacaacggca cgttccgggg
     1681 acaggagctt cttgatgtcc gtcacgatgc ccttgacgat gatacccatg cgcagtccct
     1741 tgaacccacc gccggtcgaa gcggcacttg ccccgatggc catggcaatg gttgtcgcca
     1801 gcatacccag agggccccac tcgcgtacaa acgtccgcgc atagatgttc gactggccag
     1861 tggtcgtgtg acccgagacg agctgaaaga aaccgcgccg gaacaaggca acagcgttcg
     1921 gatagactcc tcgctgcatg agtcccaggc atacgagaga gaacgtcgtg agcacggtga
     1981 tcgtgaacga gacgatctcg acgttgcgca ggatctcctt gcgattgcct ctccatattg
     2041 tgtagtgcag ggcgaagttg aacgacccca ggatgaagaa caccatgctg acgatgtcca
     2101 tgccgctgct gtggtaatac agcatgttct ggctgtaggg ggcgaacccc ccggtgctcc
     2161 aggcggacat aaagatgtat gctccgtgga agaatgcgag agacggtctg ataccattcc
     2221 ctatggcgat gaaaccgagc acgatcgtgc cgacggccag gtagatgagc gagatgcgcc
     2281 agatcaggat ggcaatgtgc cggacgttgg gctccagttt ctcttccttg ccctcgccga
     2341 ccatgatctt gaatgcgccg cctgaagagc caatgaggaa cgtcagtgcc agcaccacga
     2401 tcccctgacc gccgacgtat gtcagaagat ggcgccacat gttcagtccc tgagaaatgt
     2461 gatccaggtc ctgcagaaga aacagaccgg tggtcgtgaa gccgctcatg gtgtcgaaac
     2521 atgcgtcgag gaacgacttc atgtgcccgc tcagcatgta gggaacaccg gacagcacca
     2581 tggcgatgag ccacgatccc gcggcaatga ccatggcatg cgtgagtccc ggcgtgttcc
     2641 tcgtgcgaga aatcagcgcg aggacgtacc cgacgagcag tgtgagcacg accgagatgg
     2701 caaagtccag ggcggaagcc cattcacgga agacgagact tgtcaccatc ggcacaagca
     2761 tgagcagccc cacgccaatc gtgacgaggc ccgtgtagta gccgatgatg cggaagtctt
     2821 ctctccagac cctgcggatc atactctagc gccccacgaa gaacagccag gagatcagga
     2881 acccggcccc gatgaggcca caggtgaacg caacctcagt gagcatcccc aggagctcat
     2941 tgcggcgaga tgtcgtgcga cttatacgcc taccgagagt catggcagct atgcctgatg
     3001 gaacacttgc ctgaactgct gttcagcctc aggcgccacg agaccaacga caacgtcacc
     3061 cgcattcagc tccgttgcgc cacgagggaa gatcacgttg ccgtcatgga caacggatgc
     3121 gacgacacag ccgcggggcc atgacagctc cctcagggtc ttgcctgcag ccacagatcc
     3181 gtcagccacc ttcagctcga cgagcgtcgc cttgcccttt gcgatcgata ccagagggat
     3241 cagatcattg acgatgatgc cctcctcgat gaccgtcgcc aggatgttgg tcgagttgag
     3301 agcgaaatcc acgccgaact tgtggaagat gtcctcgttg cgagggtcgt tgatacgggc
     3361 cgtcgtaagc gggacattga agcggacctt ggcaagctga caggccacca gattgttcgc
     3421 atcatcgccg gtgacggcag caaaacgatc cgtcttctcg atcccggcgt cgatcagcgc
     3481 gctcaggtca cagcagtcgc cgttgatgac cagcgccttt ccccattcgg acaaggcttc
     3541 acaggccccg gcgtcctgct cgataacggc aacgtcatgt cccttgccga caagcaaatg
     3601 ggcaagatag gacccgacct ttcctccgcc cgcaataatg atgtacatga ctctacctct
     3661 cgtccagatg gaagatcgaa ctgacctttg gaaggtctga tacgtggagt gctatcaaca
     3721 gctgatcccc ttcggccaga acgtcgttcc ccttgggaaa cgtcgtcgtc ccaccgcgcg
     3781 tgagcgagat gaccttgaag tcgtccagtg cctcgacccc ggctatcgtg gtcgcaggaa
     3841 tactggtatg gaaggcaact tcgacgagct cgatgccgcc gcccggcaag atgaaatgac
     3901 tctcctcgtt gcggatcaga aacttgctgt agatgatatt ggcagcggac gtcgtcgggc
     3961 acacggtctc cagccccagc gcacgatacg tctcgccctt tatgggatcg aagacccgag
     4021 cgatcaccat ggggaccttg aagatggtct tggcaacctg cgcagccatg atgttcatgt
     4081 tgtcgctgtc cgtcacggcg gcgaaaccat cggcatgctc gatccccgca gacttcagta
     4141 cgtcgatgtc gaatccgact ccggtgatcg tggctccatt gaactcatcg ctgagccgcg
     4201 cgaaattgga cgaattgcga tccactaccg ccacatcatg tccctcttcg gacagcttcg
     4261 tggcgagctg agcacccagg cgcccacaac cgacaatgat aatatacatg ctggacatct
     4321 cctcgtggtc cctcggggga cccttcacaa acggatgagt atatgcaatc ccccgtacac
     4381 gtcaaggtgt ctgtcctcct tatttggaca ttccctccga gtcgtcggaa cggcctatat
     4441 gcaagtcatg gcggtgttgc aggtgtgatg caaccgtgct accataggcc gccaatattc
     4501 gccacgatga ggagacagca tgcaggaacc ggtcgagaac caagtcatca agaccagtcc
     4561 acgaggaagc cgcccgcgca gtctgcgagc tgcatccctt ggagtatggg gtcagtcggg
     4621 cgctgtcatg gcgttctacc acagcggggc gctggccgac attgtcatca tcctcggcat
     4681 tcttggtgtc ttctacggca tagcccaggt cggccgcgag tggacgggca tcctgaggcc
     4741 taccgtgctg atcgatctgt cattcggtgc tctgccccga tacacattct tctcgctggt
     4801 tcgggggctg gccgcctact gcatttcctt cctcttcact attgtctatg gattctgggc
     4861 cgccaaggac aagctggccg agagactgct tgttcccatc ctggacatcc tgcagagcat
     4921 ccccgttctg ggcttcatgc cgggcgtcgt gctggcgctg gtttcgctct tcccgaacag
     4981 caacgtcgga ctcgaactgg ccgcggtact gatgatcttc accggacagg cgtggaacat
     5041 gacgttcagc ttctacaatt ccctcaagtc ggtgccgcag tacctccagg aagccggcac
     5101 gatctaccgc ttcaactggt ggcaacgtct cacgaagatc gaaatgccct acagcgcggt
     5161 cggccttatc tggaacagca tgatgagcat ggccggcgga tggttcttcc tcatggtcac
     5221 cgaagcattc cagctgggca acagggactt ccggctgccc ggcatcgggt cctacatgtc
     5281 cgtcgcggtc gaaaagggca atgtgccggc gatggcagcg gcggtcgtcg ccatggtcct
     5341 gatgatcgtt gcgctggacc agctgctctg gcgcccgacc gtcgtctggg cacagaagtt
     5401 ccgtatcgag gacacgagcg acgaagagac gatggactcc tggttcctgg atctgctcca
     5461 ccactctcat gtcctgcgct ggatcggggc ggcggagtca tcagtcagac gtgcagtcgg
     5521 cactgcaatc tcacgggtct tccggcggac ccctcgtgag gtgcctgctg gcggtctgtc
     5581 cgccccggct cccgacgtcc atgaaacgcc aaacaggctg tctccggctg tcgtcttctc
     5641 cagggccctg ttcctgctgc tcgcggtcgg actggccttc gcactttccc agatcgttcg
     5701 tctccttgta tccgtccgca tcggtgaatg gctgcgcctg ctgagatcgg atctcctgac
     5761 cctggcacgc gtgacggcag caattgccct tggcacgctg tgggccctgc ccgtcgggtt
     5821 gcgcatcggg ctgtcgccca agctttcgag gatcatgcag ccagttgtcc aggtcgccgc
     5881 gtcgttcccg gcgcccatgc tgttccccgc ggtcatcctt gtgctcacgc tcctgcacgt
     5941 caacatcggc ttcggcagca tcgtgctcat gcttctcggc acccagtggt acatcctgtt
     6001 caacgtcatc gcaggagcac aggccatccc ctccgacctc aaggaggctg ctgcgagcta
     6061 ccgcgtgaca ggcaggcaac gcttctggtc gctgtacctc cccgccatat tcccctatct
     6121 cgtgaccggc tgggtgacgg cgacaggcgg ggcctggaac gcgagcatcg tcgccgagat
     6181 cgtgacgttc cgcggccaga cgctcagggc gcagggaatc gggtctgtca tcagtgaagc
     6241 cgcattcggc gcccacttcg ccgaactggc tgccgcgatc ctcatcatgt cactgaccgt
     6301 cgtgctcatc aaccgcttcg tgtggcgcag gctgtacgcc gtggccagca cgcagtacaa
     6361 ccttagcaag tagaggagac caccatggaa gactctgtgc agcagcccat cctgctttcc
     6421 agcgagaacg tcacccagcg tttccctatg cccaacggga acatggtgac cgttctggac
     6481 aatatcagca tggatattcg aaagggtgag atcatctgtc tcctgggccc ttcgggctgt
     6541 ggcaagtcga cgttcctgcg catcctggcg ggcctcatgc gtccgacaag cggcaaggtg
     6601 ttcgcccgag gcaaggagat ggccggcctc acgccaggcg tcggcatggt cttccagatg
     6661 ttcgccctgt ttccgtggat gaccgtgagc gagaacatcc agtcggtgct tgtccctctg
     6721 cagcttccac gtgaggaagt gcagaaccgt gtctcgaccg tcatcaagat ggtcggtctg
     6781 gagggctttg aagaggcgta tccacgcgag atctccggtg gcatgaaaca gcgtgtgggc
     6841 attgcgcggg gcctggcggt acagccggag ctgctgttca tggacgagcc gttcagcgcg
     6901 gtggatgctc tgaccgcgga gtcgctccgc acagacctgg tcgacatctg ggatgaagcc
     6961 gacggccagc cggcctcgat cgtcatggtc agtcatgaca tcaaggaagt cgtctacatg
     7021 gctgac
//
LOCUS       DATMZD010000120         2290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_33831,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000120 DATMZD010000000
VERSION     DATMZD010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33831"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="VN478_02535"
     CDS             complement(<1..672)
                     /locus_tag="VN478_02535"
                     /inference="COORDINATES: protein motif:HMM:NF013768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21590.1"
                     /translation="MFLTERQKRILLAIVRKYMDDGEPVSSGFVTSQLGNDLSSATVR
                     NEMADLSSMGYLQQPHVSAGRVPTVLAYHMYISAFLVEATRQRMFTGPMDLPLQYESL
                     PRLFDGVATAVSDRTKKVSFVLSPPINSVTLRHVSLVPFSETVLMLVFITDRENVEGY
                     KLLAPADTTEQELADINAIFARELRGKSLEEGIRTLRFGNVLPQDLAIRYGGVLDAVA
                     ELMEGE"
     gene            complement(777..1112)
                     /gene="trxA"
                     /locus_tag="VN478_02540"
     CDS             complement(777..1112)
                     /gene="trxA"
                     /locus_tag="VN478_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HWQ21591.1"
                     /translation="MEDNEIVATTANFQSEVLSSSLPVLVDFWATWCAPCRMIAPVVT
                     EIAGDYAGKLKVARLNVDDNQEISARYGVMSIPTLALFKGGVIVDQVVGAVPKRTITD
                     KIDALLQKS"
     gene            complement(1164..1418)
                     /locus_tag="VN478_02545"
     CDS             complement(1164..1418)
                     /locus_tag="VN478_02545"
                     /inference="COORDINATES: protein motif:HMM:NF014624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HWQ21592.1"
                     /translation="MEKTDILHSLRRVEGQLRGIQKMVDEGRPCDDILAQLVAAHAAI
                     GRIGTDVLLNEVGCHTQPERAPELELERLEKLLVTYGGLK"
     gene            1506..1838
                     /locus_tag="VN478_02550"
     CDS             1506..1838
                     /locus_tag="VN478_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21593.1"
                     /translation="MKRVLTSTVLLLVVFVVFALVVGQFRDKPVALTVPFFATMHPSV
                     EAVAYVSFFIGLLLATVIALAGDVALRKRFRAMTLDQSRHSKTSGGSDEIADTKAATV
                     TSEHKPQG"
     gene            1835..>2290
                     /locus_tag="VN478_02555"
     CDS             1835..>2290
                     /locus_tag="VN478_02555"
                     /inference="COORDINATES: protein motif:HMM:NF017941.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HWQ21594.1"
                     /translation="MSRHSTAQITRAALIAAMYIVFTMLPPFNAISYGAIQFRLGEAL
                     VLLPFLLDEAVVGVVTGCLVANLFSPFGIIDVVIGTGVTTVAALLTRRLRSTGRLWLA
                     ALPPIVLNALIVPAYVSTLTLPGAQDLPVAHSLTAGLRFILAHFSWGVYM"
BASE COUNT          465 a          697 c          631 g          497 t
ORIGIN      
        1 ttctccttcc attagttcag ccacagcgtc gaggacgcct ccatagcgga tggccaggtc
       61 ctgcgggaga acattgccga agcgaagcgt ccggataccc tcctcgagac tcttcccgcg
      121 gagttccctg gcaaagatcg cattgatgtc cgcaagctcc tgctcagtcg tatccgccgg
      181 cgcgagcaac ttgtacccct ccacattctc gcggtcagtg atgaagacca gcataagcac
      241 agtctcgctg aacggcacca ggctgacgtg acggagggta accgagttga tgggaggact
      301 gaggacaaag ctcaccttct tcgttcggtc ggacaccgca gtcgccacgc cgtcgaacag
      361 gcgcggaaga ctctcatact gcaagggaag atccatcggt ccggtgaaca tgcgctggcg
      421 cgtcgcctcg accaggaacg ccgagatgta catgtgatag gccagaacgg tgggaacgcg
      481 ccccgccgac acgtggggct gctgaagata tcccatgctg ctaaggtcag ccatctcgtt
      541 gcggactgtc gcagacgaga gatcgttgcc cagctgactg gtcacgaatc ccgaactgac
      601 cggctcgccg tcatccatgt acttccggac gatagccagc aatatgcgct tctgccgttc
      661 tgtaaggaac atctagctca ccctttctat tgtaccgaat agcgccggac tcgcaagcaa
      721 ggagcgggcg gccaccgcta tggcaaccac ccgctccgca agaatgtgaa ggattcctag
      781 ctcttctgga gcagagcgtc gatcttgtct gtgattgtcc tcttgggcac cgcgcccact
      841 acctgatcga cgatgacacc gcccttgaac agcgcaagtg tcgggatact catgactcca
      901 tagcgtgcag agatctcctg attgtcgtcc acgttcaagc gggcaacctt cagcttccct
      961 gcatagtcac cggcaatctc ggtgacgact ggggcaatca tccggcatgg agcgcaccac
     1021 gtggcccaga agtcgaccag gacgggcagc gaagaagaga gtacctcgct ctggaaattc
     1081 gcagttgttg caacgatctc attgtcttcc atgtgttcct ccctatgaga ctgaatgtcc
     1141 aacaccgtac gtattcgaag accctacttg agtccaccgt acgtgacaag aagcttctcc
     1201 agacgctcca gttccagctc aggggcacgt tccggctgcg tgtggcagcc gacttcgttg
     1261 agaaggacgt ctgttccgat gcggcctatg gcagcatgtg cagccacgag ctgtgcaagg
     1321 atatcatcac atgggcggcc ttcgtcgacc attttctgaa tcccgcgcaa ctgcccttcg
     1381 acgcgtcgaa ggctatgcag aatgtctgtc ttctccataa tatacccctt ggggtatact
     1441 atgcagatag ttgtcgttgt caagaatgcc tgtactatag tagaacaagt ggttggaggt
     1501 gttatgtgaa gcgagttttg accagtacgg tccttcttct tgtcgtgttc gtcgtcttcg
     1561 cacttgttgt cggccagttt cgagacaagc ctgttgcgct gacagtaccg ttctttgcca
     1621 cgatgcaccc gtcagtggag gcagtggctt atgtctcctt cttcatcggc ctgctgcttg
     1681 ccacagtcat cgcgctcgcc ggcgatgtag cgctccgaaa gaggtttcgc gcgatgacgc
     1741 tggaccagag cagacactcg aagacgtctg gcggaagtga cgagatcgcg gataccaaag
     1801 ctgcgactgt caccagcgag cacaagccac aaggatgagc cgtcacagca cggcacagat
     1861 cacgcgcgcc gcgctcatcg ccgcgatgta catcgtcttc acaatgctcc cgcctttcaa
     1921 cgctatcagt tatggagcca tacagttccg gttgggggag gccctggttc tgctgccctt
     1981 tctcctcgac gaagcagtag tgggcgtggt caccggttgc cttgtcgcta acctcttctc
     2041 gccgttcggc atcatcgacg tcgtcatcgg taccggggtg actacagtgg ccgcactcct
     2101 tacaagacgc ctgcgcagca ccggcagact gtggctggcg gccctaccac cgatcgtgct
     2161 caacgcactc atcgtgccgg catacgtttc gacactcacg ctgccgggag cgcaggacct
     2221 gccggtcgcg cacagcctca cggccggtct gcgcttcatc ctcgcccact tctcgtgggg
     2281 tgtctacatg
//
LOCUS       DATMZD010000121         4991 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_43510,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000121 DATMZD010000000
VERSION     DATMZD010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4991
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43510"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3305)
                     /locus_tag="VN478_02560"
     CDS             complement(<1..3305)
                     /locus_tag="VN478_02560"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWQ21595.1"
                     /translation="MKKTADSAELTRLMADLPGMVYQAAAQAPFAFRLVGGGYERILG
                     RSSDELTADSGIRPTLMYPDDLARYQSVVESAVRDDRAFEVEYSVIHADATERVVWEQ
                     GRPVQMPDGSWLIEGSIVDIEGPVHARRVQDATYRISQAAVSAESLQELYSQIHHIIS
                     ELMPSENLYIALRDPTTGFITYPYYVDQYDVVPPDPQTAETGLTAHILRTGQPLLLSE
                     FVESEPIRSGDLTPTGTPPVSWLGVPLRPQGRPMGVIAVQVYSGRYRYTDRDRDILSF
                     VADQIAIAIERRRAEEFLQESELRYRSLFEDSPVALWEEDFSGLRKRLTQLLPQGTTD
                     VASWLHSRRDIVRELVSLVQVTDVNRAALALAGASQKSELIGSLGSSMPDDALDRFEL
                     QLVHIANGDLSFRWEGPASTVRGTARSISIQWMVAPGDEDNLRRVRVAMSDITDRLAA
                     ENSLRDSEARMRALFAAMNDVIIILDRDGQCLEAVPTGASPGYSVDSLIGRNTRDVFA
                     PDVAKAILRQVERALSEHVTTEVEFNRDIGGAPHWLEVRISPLTDERALLITHDATSR
                     HSAQEAARVESSKAESYFNTSGVIMEVTDLDLRLVRLNRKGYEFFGYTPDEIVGRDWF
                     DIKLPEREREKTKALIRRELAGGFLPNYTEGPVVTRTGEERIVAWHDSLLRDSEGRVI
                     GLVSSGVDVTDRVHAERASQANELKFRNVFNQAQDAMVLHAINADGTTSPFLEVNDVA
                     CQWSGYTRDELQHMTIRDLVVASMHDTLIQASKQLTEQGKATFSVELKARDGHRFPTE
                     VNSSVFTLEGRTVVLSIVRDITERMRLEAELQRMDKLESLGTLAGGIAHDFNNMMTGI
                     IGNINLARIEDDRGRARVLLEEAEREVLRARGLTQQLLTFAKGGAPVRSVQDVSHILR
                     ESVGFALRGSDVSASFDLPADLSWVDVDGGQIGQVFSNIAINADEAMPTGGTLTVSAC
                     NVTFAEPTPYPDLVPGNYVRIDISDAGVGIAEADLRKIFDPFFSTKRRGSGLGLATSY
                     AIVHKHDGCIGVRSTPGVGTTFSIFLPAVPARPSPRPADTKTGFSGHGRVLVMDDELT
                     VRQVVVAMLKRLG"
     gene            complement(3326..3622)
                     /locus_tag="VN478_02565"
     CDS             complement(3326..3622)
                     /locus_tag="VN478_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21596.1"
                     /translation="MRRQSWWWLLLAAFVLAVLNLIFNGWQFLVPGALLFFASNILSM
                     GLSIAAIVWSILDLLACYRTGRPIKAGIYVTSWVLATFVVVAVDLWTLSVLRSR"
     gene            3787..4086
                     /locus_tag="VN478_02570"
     CDS             3787..4086
                     /locus_tag="VN478_02570"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HWQ21597.1"
                     /translation="MATWIYLLHLTRPEAVSSPTEDESAAIDRHFVRLQRACAAGTVR
                     LAGPATDGAFGIVIFEARDEAAACSFMVEDPAVRAGVMTAELHPFRVSLEHLPCP"
     gene            4077..4682
                     /locus_tag="VN478_02575"
     CDS             4077..4682
                     /locus_tag="VN478_02575"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HWQ21598.1"
                     /translation="MSVMGVELLPVLGYAVASIFTPGPNNITSSSLGMRVGYPRALRF
                     IAGVVTGFFCIMVAAGLLTELLVSVYDRMAVVLKIAGVAYLLWIAWTVVRPEDRKHES
                     PAAGGTRYRDGLMLQFVNPKVILFGLTMYATLLVPLAKNWLAVVISAVLLAILSFCST
                     SMWALLGAGIQRFIANPKVKFAYSVVLVGLLLYAAWSIVAS"
     gene            complement(4736..>4991)
                     /locus_tag="VN478_02580"
     CDS             complement(4736..>4991)
                     /locus_tag="VN478_02580"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HWQ21599.1"
                     /translation="CWMAPFMMIGRGIRNFVHLPAVQLRANTSACVSCHACTTACPMS
                     LEVQEMVLTQRMENSECILCGTCVDVCPRKAIRYDFGKRQ"
BASE COUNT          915 a         1480 c         1589 g         1007 t
ORIGIN      
        1 tatccgagtc tcttgagcat cgcgaccacg acctgacgca cggtgagttc gtcgtccatt
       61 accaggacac ggccgtgccc gctgaaccct gtctttgtgt cggcgggccg aggagatggc
      121 ctggcaggga cggcaggcag gaagatggag aaggtcgtgc cgacgcctgg tgtcgagcgg
      181 acgccgatgc agccgtcatg cttgtgaacg atggcgtagg acgtcgcgag gccgagtccg
      241 ctgcctcggc gtttcgtgct gaagaacggg tcgaagatct tgcgcaggtc tgcctccgcg
      301 atgccgaccc ccgcgtcgct gatgtcgatg cggacgtagt tgcccggaac caggtccgga
      361 tatggcgtcg gttcggcgaa tgtgacgttg catgccgaga cggtgagagt tccgccggtg
      421 ggcatcgctt catcggcgtt gatcgcaata ttcgagaaga cctgtccgat ctgaccgccg
      481 tcgacatcca cccacgagag gtcggcaggc aggtcgaatg aagcagaaac gtcagaacca
      541 cgcagtgcga agccgaccga ttcccggagg atgtgggaga cgtcctggac ggaacgcacc
      601 ggagcgcctc ccttcgcgaa cgtcaggagc tgttgcgtaa gaccgcgggc acgaaggacc
      661 tcccgctctg cttcctcgag aagtacgcgc gcgcggcctc tgtcatcctc gatgcgggca
      721 aggttgatgt tgccgatgat cccggtcatc atgttattga aatcatgtgc gatgccgccg
      781 gccagcgtgc ccagggactc cagcttgtcc atccgctgga gctcagcttc gagacgcatt
      841 cgctcggtga tgtcacgaac gatggagaga acgaccgttc gaccctccag tgtgaacaca
      901 cttgagttca cctccgtcgg gaaccggtgg ccgtcccgag ccttcagctc gaccgagaag
      961 gttgcctttc cctgctccgt caactgcttc gatgcttgta tgagagtgtc gtgcatcgat
     1021 gcgaccacga ggtctctgat agtcatgtgc tgcaattcat cacgcgtgta gcctgaccac
     1081 tggcacgcaa cgtcgttgac ttccagaaac gggctggtcg ttccatcggc gttgatggcg
     1141 tggaggacca tggcgtcctg ggcctggttg aacacatttc tgaacttcag ttcgttggcc
     1201 tgcgacgcgc gttcggcatg gacacggtcg gtgacgtcga cgccagagga gacaagcccg
     1261 atgactcggc cttcagagtc gcggagcagg gagtcgtgcc aggcgacgat gcgctcttct
     1321 cctgtgcggg tgacgaccgg cccctcggtg tagttcggga ggaacccccc ggcgagctcg
     1381 cggcggatca gcgccttggt cttctcgcgt tcgcgttccg gcagcttgat gtcgaaccag
     1441 tcccgtccga cgatctcgtc tggagtgtac ccgaagaact cgtagccctt ccgattgagg
     1501 cggaccagac ggaggtccag gtctgtgacc tccatgatga cgcctgatgt gttgaagtag
     1561 ctctcagcct tggacgattc cacgcgtgcg gcttcctgtg cgctgtggcg cgatgtcgcg
     1621 tcgtgggtga tcagcagggc ccgttcatcc gtgagtgggc tgatccggac ctccagccag
     1681 tgtggagctc ccccaatgtc gcggttgaac tcgacctcgg tggtcacatg ttcggagagt
     1741 gccctctcga cctggcggag gatcgccttg gcgacatcgg gggcgaagac gtcgcgtgta
     1801 ttcctcccga tcagactgtc cactgaataa cccggcgatg ctcctgtcgg tactgcctcc
     1861 aggcattgac cgtcgcggtc caggatgatg atgacgtcgt tcatggctgc gaacagggca
     1921 cgcatgcgtg cctcggagtc tcgcagcgag ttctcggctg cgagacggtc tgtgatgtcg
     1981 gacatggcga cgcggaccct ccgcagattg tcctcgtcac ccggcgccac catccactgt
     2041 atcgaaatgg acctggcggt gccacgtacc gtggaggccg gtccttccca ccggaaggag
     2101 aggtccccgt tggcgatgtg gaccagttga agctcgaagc ggtcgagagc gtcgtcaggc
     2161 atactggagc caagagaccc tatcagttcg cttttctggc tggcgccggc cagggcaagg
     2221 gcagcgcggt tgacgtcggt gacctggacg agcgagacga gctccctgac gatgtcgcgg
     2281 cgagagtgca gccacgatgc aacatccgtg gttccctgcg gcagcaattg cgtcaggcgc
     2341 ttgcgaagtc cggagaagtc ctcctcccag agcgcgactg gcgagtcctc aaagagcgag
     2401 cgatagcgca gttcgctttc ctgaaggaac tcttcagcgc gccgacgctc gatggcaatc
     2461 gcgatctggt cagcaacgaa cgagagaatg tcccggtcgc ggtcggtata ccgatacctg
     2521 ccgctgtaaa cctgaacggc gatcacgccc atgggccttc cctgcggtct cagggggact
     2581 ccaagccagc tgacaggagg cgttcccgtg ggggtcaggt cacccgacct gatcggctct
     2641 gactcgacga actcgctgag aaggagtggc tgcccggtcc gcaggatgtg tgccgtaagt
     2701 cctgtttcgg ccgtctgcgg gtcgggaggc acgacgtcat actgatcgac atagtaggga
     2761 tacgtgatga atcctgtcgt cgggtcccgg agggcgatgt acaggttctc cgacggcatt
     2821 agttcactga tgatgtggtg gatctggctg tacagctcct gcagactctc ggcggagacg
     2881 gcagcctgtg agatacgata cgtcgcatcc tgcacccgcc gcgcatgaac cggtccctcg
     2941 atgtcgacga tgcttccctc gatgagccag gacccgtcgg gcatctggac gggtctgccc
     3001 tgttcccaca caacgcgttc tgtggcgtca gcgtgaatga cgctgtactc cacctcgaac
     3061 gcgcgatcgt ctctcactgc gctctcgaca actgactggt agcgagcgag gtcatcggga
     3121 tacatgagcg tcgggcgaat gccgctgtcg gctgtcagtt cgtccgatga tcggccaagg
     3181 atgcgttcgt agcctccgcc gaccagtctg aaggcaaacg gtgcctgtgc tgccgcctgg
     3241 tagaccatac caggcaggtc cgccatcagg cgggtcagtt ctgcgctgtc cgcagtcttc
     3301 ttcatgagag gctcatcact gtcgttcatc tcgaccgcag gacggacagg gtccacaggt
     3361 caacagcgac gacgacaaat gtcgccagca cccatgacgt cacgtagatg ccggccttta
     3421 tgggacgtcc ggtcctgtag caagccagca agtccaggat agaccatacg atggctgcaa
     3481 tcgacaatcc catcgaaagg atgttgctcg caaagaacag caaggcgccc ggaacgagga
     3541 actgccatcc attgaaaatc aggttgagga cggccaggac gaatgcagcc agcagcagcc
     3601 accaccatga ttgtctacgc atcgccaccg tgtcccctgc acatagttga tagtatggtg
     3661 gagaaccgga agggtgcaag gagaatgccg cggcgcaccg ggaagtgttg tcagaaagac
     3721 tcgacagcaa acctgctttc ctgctacact gttgccagag ggggagccgt gactcgggag
     3781 aatgacgtgg cgacatggat ctatctgctg cacctgacca ggccggaggc cgtgtcgtcg
     3841 ccgacggagg acgagtctgc cgcaatcgac cgtcacttcg tgcgcctgca gcgggcgtgt
     3901 gctgccggta ccgtgcggct ggcagggccg gcgacggacg gggcattcgg catcgtcatc
     3961 ttcgaagccc gggacgaggc agctgcgtgc tccttcatgg tggaggatcc agctgtgcgt
     4021 gcaggagtca tgaccgccga gctgcacccg tttcgcgtct cgctggagca tctgccatgt
     4081 ccgtgatggg ggtcgagctg ctgcccgtgc ttggctacgc ggtggcatcc atcttcactc
     4141 ccggcccgaa caacatcacg tcttcgtcgc tggggatgcg cgttgggtat ccacgtgcgc
     4201 tgcgcttcat agcgggagtc gtcaccggat tcttctgcat catggtggca gcgggactgc
     4261 tgacggagct cctcgtctcg gtctacgatc gcatggcggt cgtgctcaag atcgcaggcg
     4321 tcgcgtacct gctgtggatc gcatggaccg tcgtccgacc ggaagacagg aagcatgagt
     4381 ccccggcggc gggcggcact cgataccgtg acgggctgat gctccagttc gtcaacccca
     4441 aggtcatcct gttcgggctg acgatgtatg cgacgctcct ggtcccgctg gcgaagaact
     4501 ggctggccgt cgtcatctct gcagtgttgc tcgctatcct gagcttctgc tcgacctcga
     4561 tgtgggcgtt gctgggtgcc ggcatccagc ggttcatcgc caacccgaag gtcaagtttg
     4621 catactcagt cgtccttgta ggacttctgc tctacgccgc ctggtcgatc gtcgcatcgt
     4681 agacagcttg tttcctggat cgtgaattcc cttgtatcag gtacaacggg attcactatt
     4741 gacgcttccc gaaatcgtag cggatggcct tgcgggggca gacgtcgacg cacgtgccgc
     4801 agaggatgca ctcgctgttc tccattctct gcgtcagaac catttcctgg acctcgagac
     4861 tcatagggca tgcggtcgtg caggcatgac agctgacaca tgcggacgtg ttggcgcgaa
     4921 gctggacggc gggaaggtga acgaagttgc ggattcccct gccgatcatc atgaaaggcg
     4981 ccatccagca g
//
LOCUS       DATMZD010000122         2735 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_104152,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000122 DATMZD010000000
VERSION     DATMZD010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104152"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="VN478_02585"
     CDS             <1..658
                     /locus_tag="VN478_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006569743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWQ21600.1"
                     /translation="ALSDVTLEVPEGHIMGLLGPNGSGKTTLMKILAGLVSPTSGTVL
                     IDGIAPGVRTKAIVSFLPDVNHLDRWMSVGEAGDFYADFYADFERERFEELLGIMHIT
                     SGQKIGSLSKGMVEKVRLSLVFSRRARLYVLDEPFGGLDPLARSQVLDALVANFRDDC
                     SMVLSTQMVADVERFFDDVVFLDQGRVVLAGEAEDLRTRRGGSIEDIYREVYGHVEAR
                     "
     gene            642..1427
                     /locus_tag="VN478_02590"
     CDS             642..1427
                     /locus_tag="VN478_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21601.1"
                     /translation="MWKLVRYQLRGRRLLYTIGLITMGALTLYQATRYFGMHENPAQS
                     APVSMSVPLVMALFWVAFYAVMTVVNVVTYTQDLYGDTGYLVFSLPLRGAEVLSSRIV
                     LLVFDTIVTLLGGMASVIVLACLAPGLAQQVRPWVGRFLLTGDYWVMCLGVLAVIVSF
                     MSIMFFGVTFGKTLTNSKKHLGSVFSALVVIAVLWASAQLGSLLETRLFEGIHLTLNP
                     WRNLPILGPATIQFPLVSPLMFVTLGIVAFAVTSWLMDRKLNL"
     gene            1533..2435
                     /locus_tag="VN478_02595"
     CDS             1533..2435
                     /locus_tag="VN478_02595"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HWQ21602.1"
                     /translation="MKRANESGTTASIQPRIKSITCALTPAERLPPMSLDPMTLHIPP
                     QILAVTGPVVHRSSPHHGRSGLVLLLQNASDQRFVLKVARGAYRMQELALEYDVLKLL
                     HGTEVPVPRALAHVQNRPLGFLLLGQVAGRSATDVLREAGSSDDRDRIARALGSMLST
                     LHHLPVGAASWQDCVDGQLLWAERHLQEKVMSRAEFRAKGITGDPVKELEHLKETCPA
                     PGTVAVLHGDFRPRNVLLEEGRITSVLDWSFTDLGDPWYDLATMFGYLDLDGQQVFLN
                     AYGLEAVDRKRLAWFRSLSVYLAV"
BASE COUNT          475 a          789 c          864 g          607 t
ORIGIN      
        1 tgcgctttca gacgtcacgc tggaggtgcc ggagggacac atcatgggac ttctcggtcc
       61 caacggcagt ggcaaaacga cacttatgaa gattctcgca ggtctggtct cgccgacgtc
      121 ggggactgtt cttatcgacg gcatagctcc gggcgtcagg accaaggcga tcgtctcctt
      181 tctgccggac gtgaatcacc tggatcggtg gatgtcggtc ggagaagcag gggactttta
      241 tgcggacttc tacgccgact tcgagcgaga acgattcgag gagctgcttg gcatcatgca
      301 catcacttcc ggccagaaga tcggctcgct gtccaaagga atggtggaaa aggtgaggct
      361 gtcgctggtc ttctcccggc gggcgcgact atatgtcctg gatgagccct ttggaggatt
      421 ggacccgttg gcgcgcagcc aggtcctgga tgctcttgtg gccaacttcc gcgacgactg
      481 ctccatggtg ttgtcgacgc agatggtcgc ggacgtcgag cgattcttcg atgacgtcgt
      541 gttcctggac caggggcgtg tcgtcctggc gggcgaagcg gaggacctgc gcaccaggcg
      601 gggaggatcg atcgaggaca tctacaggga ggtatacgga catgtggaag ctcgttaggt
      661 atcagctcag gggccgcaga ctcctctaca ccatcggact catcaccatg ggcgcgctca
      721 cgctctatca ggcgacgcgc tacttcggaa tgcacgagaa ccctgcgcag tcagcgcctg
      781 tctcgatgag cgtgccactt gtgatggctc tgttctgggt ggccttctat gctgtcatga
      841 cggtcgtcaa cgtcgtgaca tacacacagg acctgtatgg cgacaccggc taccttgtct
      901 tcagtctgcc gttgcgggga gcggaggtcc tgagttcgcg gatcgtcctt ctcgtgttcg
      961 acacgattgt gacgctgctg ggcggcatgg cgagcgtgat cgtccttgct tgtcttgctc
     1021 ccggacttgc tcagcaggta cggccctggg tcggcaggtt cctgctgacg ggcgactact
     1081 gggtgatgtg tctcggcgtc ctggcggtga tcgtcagttt catgtcgatc atgttctttg
     1141 gcgtgacgtt cggcaagaca ctgacgaact cgaagaagca ccttggaagt gtcttctccg
     1201 cgcttgtcgt gattgccgtg ctgtgggcca gtgctcagct ggggagcctg ctcgagacac
     1261 gcctgttcga ggggatccac cttacgttga acccctggcg gaatctgccg attcttgggc
     1321 ctgcgacgat ccagttcccg ctggtctcgc cgctgatgtt cgtcacactt ggcatcgtgg
     1381 cgttcgccgt gacgtcctgg ctcatggacc gcaagctcaa cctgtaggcg gtgattcgaa
     1441 tgcggtgggc ccctgaaccg caggggtcca ccattgtcaa tgttgtgtga gtgtgttttg
     1501 ttcaatacac caaatgatgg tatcctgtat ctatgaaacg tgctaacgaa tctggtacga
     1561 cagcgtccat acaaccacgc atcaagtcca tcacgtgtgc tcttacgccg gccgagcgct
     1621 tgcccccgat gagcctggat ccgatgacgc tgcacatacc ccctcagatc ctggcagtga
     1681 ctggaccagt ggtgcacaga tcgtccccgc accatggcag aagcggtctg gtgctgctgc
     1741 tgcagaatgc gtcagaccag cggttcgtgc tcaaggtggc ccgcggagcg taccgcatgc
     1801 aggaacttgc gctggagtat gacgtgctga agctgctgca tgggacagag gtcccagtgc
     1861 cgagggcact tgcccacgtc cagaatcgac cgctcggatt cctgttgctg ggacaggttg
     1921 ccggtcgctc tgcgactgac gtcctgcgcg aggctggttc cagcgacgat cgcgatcgca
     1981 tcgccagggc cctgggttcc atgctgtcga cgctgcacca tctgccggtc ggagcagcaa
     2041 gctggcagga ctgcgtcgac ggacagctcc tctgggctga gcggcatctt caggagaagg
     2101 tgatgagcag ggccgaattt cgagccaaag gcatcacagg cgaccccgtc aaggaactcg
     2161 agcacttgaa ggaaacctgt ccagcgcctg gcaccgtggc ggtactccac ggtgacttcc
     2221 ggcccaggaa tgtgctgctg gaagagggtc ggatcacgtc tgtcctggac tggtccttca
     2281 cggacctcgg cgacccctgg tacgatctcg caacaatgtt cgggtatctg gacctcgacg
     2341 gccagcaggt gttcctgaac gcgtacgggc tcgaggcggt cgatcgcaag cgcctggcct
     2401 ggttccgttc gctctctgtc tacctcgctg tctgaagccc cgcctacagg tccggcgagc
     2461 ctttgtgccc ccgcctgatg cgtgaaggat gggactgacg tgacgtctcg ggcttctgcc
     2521 tcgtggtgtc aggacgtgtt ggtgttgtct cagtgtccat gttgcctccc ttccggtttc
     2581 tcgtgctgag gaatcatcct ctcaggaaaa tactatcgga ggtgggcgcg atcggttaca
     2641 ccggcaggcg tcggcctgca gcgatcagtc tccccgcgat gagagtccgc cgataggcat
     2701 tttccactga gcccgcagga gggtgatgtc ccgcc
//
LOCUS       DATMZD010000123         2651 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_13803,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000123 DATMZD010000000
VERSION     DATMZD010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13803"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="VN478_02600"
     CDS             complement(<1..478)
                     /locus_tag="VN478_02600"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWQ21603.1"
                     /translation="MYLKSLHIQGFKTFEQSTTLELSYPVIAIVGPNGSGKSNLVDAL
                     RWTLCEQNLRTLRARSMLDLIFSGSATRHPVNYAEAEVVFNNEGDVFGTGTDKVTVAR
                     RIFRSKESDFLIDGNGVQLRDVEELLTSTGLGFRHYPFIGQGEMSRIFTMKPEERKG"
     gene            631..>2651
                     /locus_tag="VN478_02605"
     CDS             631..>2651
                     /locus_tag="VN478_02605"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HWQ21604.1"
                     /translation="MTPIELDPIYEAKKHEEEIYSLWLSQAAFHSEPNPDKTPFVVMM
                     PPPNVTGYLHIGHALNMTLQDIAIRYKRMKGFETLWLPGMDHAGIATQAVVERQIKEQ
                     GLTKEGLGRDEFVRRIWEWKDKYGGIILSQLKKLGVSADWDRLRFTLDEPYERAVKAA
                     FVHYYEKGLLYKGQRITNWCPHCHTAISDIEVTYANEKGHLWYVKYPFADGSGFVTIA
                     TTRPETILADAAIAVNPADPRYAGIVGRQVIVPVANRTIPVIADEAVLLDFGTGALKI
                     TPGHDPTDFEVGKRHDLSSMAVIGLDGLMNQNAMHYSGMTAIEAREKIVAEIDALGLL
                     EKVDEYEHSVGHCSRCSSTIEPIVTEQWYLRMKDLAARALQAEREGRVEVLPVKWRKV
                     YEGWLENINDWCVSRQLWWGHRIPVYHCEDCGEVIVSIDAPHICPKCGGSHLNQESDV
                     LDTWFSSALWPFATLGWPEQTGSLKYYYPTSLLITGYDIIFFWVARMVMSGLEFMDDV
                     PFHTVFITGLVRDEQGRKMSKSLKNQVDPLDLISTYGTDALRFTMASLSTYGGQDILL
                     SDQKLLSSRNFINKLWNATRFVVANLDVRQTLSEPRPSDDQLGLFDRWILTRLADATR
                     DVTAKMDVYDFGEASHRLYEFVWGDFCDWYIELAKIPLRDETRAPATRWV"
BASE COUNT          556 a          798 c          778 g          519 t
ORIGIN      
        1 ggcccttgcg ctcctcgggc ttcatcgtga agatgcgact catttcaccc tgcccgatga
       61 atgggtagtg gcggaacccg agtcccgtcg atgtcagcag ttcctcgaca tccctgagtt
      121 gaacaccgtt tccgtcgatg aggaagtccg actccttgga gcggaagatg cgccgggcca
      181 cggtgacctt gtccgttccg gtgccgaaca cgtcgccctc gttgttgaac acgacctcgg
      241 cctcggcgta gttgacggga tggcgtgtcg cgctgccgga gaagatcagg tcgagcatcg
      301 acctggcgcg gagcgtcctc aggttctgtt cgcagagggt ccagcgcagt gcgtcgacga
      361 ggttgctctt gcccgagcca ttgggcccga caatagcaat gacgggatat gagagctcca
      421 gcgtggtgct ctgctcaaag gttttgaacc cctggatatg aagggatttc agatacatgt
      481 cgcgcgtgtc ctccgactca gaagggtccc gtccagcaaa agtatagccg ccttcgaggc
      541 cgtggcaatc aggactgaag agcctcagtt caattgacaa acagtacccg gagcctatac
      601 ttttcggata ccgtcgtgga ggtatcagcg atgactccaa tcgaactcga tccaatatac
      661 gaggcaaaga agcacgagga agagatctac agtctgtggc tcagccaggc ggcctttcat
      721 tcggagccca accccgacaa gaccccattc gttgtcatga tgccacctcc caacgtcacg
      781 ggatacctgc acatcggaca cgctctcaac atgaccctcc aggacatcgc gatccggtac
      841 aagcggatga aggggttcga gacactgtgg ctgccaggca tggatcatgc aggcatcgcc
      901 acgcaggccg tcgtcgagcg tcagatcaag gaacagggcc tgaccaagga aggacttggc
      961 cgcgacgagt ttgtccgccg catctgggag tggaaagaca agtacggagg tatcatcctg
     1021 tcccagctca agaagctcgg cgtctctgcc gattgggatc gtctgcgctt cacgcttgat
     1081 gagccgtatg agcgcgcagt caaagctgcc tttgtccact actacgagaa gggcctcctc
     1141 tacaagggcc agcggatcac caactggtgc ccccactgcc acacggccat ttccgacatc
     1201 gaggtcacct acgccaacga gaagggccat ctctggtacg tgaagtatcc gtttgccgac
     1261 ggctccgggt tcgttacaat tgccacgaca cgaccggaaa ccatcctcgc ggatgcagcg
     1321 atcgccgtca accccgcaga cccacgctat gcaggcatcg tcggcagaca ggtcattgtc
     1381 cccgtcgcca acaggaccat ccccgtgatc gcggacgaag ccgtcctgct ggatttcggt
     1441 acgggggctc tcaagatcac gcccggccac gaccccaccg attttgaggt cggcaagcgg
     1501 catgaccttt ccagcatggc cgtcatcggc ctcgacgggc tcatgaacca gaacgcaatg
     1561 cactacagtg gcatgacagc gatagaagcc cgcgagaaga tcgtcgcgga gatcgatgcc
     1621 ctggggctcc tggagaaggt ggacgagtat gagcacagtg tgggccactg ctcgcgctgc
     1681 agctcgacca tcgagcccat tgtcaccgag cagtggtacc tccggatgaa ggaccttgct
     1741 gcaagagctc tgcaggctga acgcgagggc agggtcgagg tactgccggt caagtggcgg
     1801 aaagtgtatg agggatggct ggagaacatc aacgactggt gcgtttcgcg ccagttgtgg
     1861 tggggtcacc gcataccggt ctatcattgc gaagactgcg gcgaggtcat cgtctccatc
     1921 gatgccccgc acatctgccc gaagtgcggt ggcagccatc tcaatcagga gagcgatgtc
     1981 ctggatacgt ggttcagctc tgccctttgg ccgttcgcca cgctggggtg gcctgaacag
     2041 accggctctc tcaagtacta ctacccgacg tctctcctca tcaccggcta cgacatcatc
     2101 ttcttctggg ttgcacgcat ggtcatgtcc ggcctcgagt tcatggacga cgttcccttc
     2161 catacggtct tcatcaccgg ccttgtccgc gacgagcagg gacgcaagat gagcaagtcg
     2221 ctcaagaacc aggtggatcc gctggacctg atcagcacct atggaaccga tgccctgcgg
     2281 ttcacgatgg catcgctctc cacgtacgga ggacaggata tcctgctgtc ggatcagaag
     2341 ctgctgtcgt cgcggaactt catcaacaag ctgtggaacg ccacgcgatt cgtcgtagcg
     2401 aacctggacg tcaggcagac actgtccgaa ccgcgtccat cggacgatca gcttgggctt
     2461 ttcgatcgat ggatcctgac gcgcctggcc gatgcaacgc gcgacgtgac ggccaaaatg
     2521 gatgtgtacg acttcggcga ggcttcccat cgactctacg agtttgtctg gggcgacttc
     2581 tgcgactggt acatcgagct cgcgaagata cctctccgcg acgagaccag ggcgcccgcg
     2641 acccgctggg t
//
LOCUS       DATMZD010000124         2365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_13824,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000124 DATMZD010000000
VERSION     DATMZD010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13824"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..943)
                     /locus_tag="VN478_02610"
     CDS             complement(<1..943)
                     /locus_tag="VN478_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HWQ21605.1"
                     /translation="MATFAKDFRFGVATAAYQVEGAAAEDGRTPSIWDTFCRTPGKVH
                     GGETGDVACDNYHRYREDVRLMKEMGVDSYRFSIAWPRIFPAYGTYNPKGMEFYRSLI
                     HELQESGIDPVVTLYHWDLPNWAHERGGWLNRESVAWFAEYAAKVFEKLGDSVKLWIT
                     HNEPFCASMLGYGMGIHAPGHQDMREALIAAHHILLSHGAAVEAFRNCGLRDGKVGIT
                     LNLTPAVPFTRSRADLEAVSRSDGIANRWFLDPVLKGSYPEDTREVLRKLVGDLDFIR
                     DGDLQRISHGIDFLGVNYYTRSLVRVVQNGALNYEEVR"
     gene            complement(983..>2365)
                     /locus_tag="VN478_02615"
     CDS             complement(983..>2365)
                     /locus_tag="VN478_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HWQ21606.1"
                     /translation="GGIYESEQFYALCDELGIMVWQDFMFACAEYPDHLAWFRRLCNT
                     EVRETVRQLRHHASIVLWCGNNENNMGFDEWPMMAHKVDGEYLGNRLYLHDFPLICAR
                     EDPSRPYWPSSPYGGDKANAAGYGDRHVWNVWSGWADYRSYADEDGRFISEFGFESAP
                     DARTIDFFATSEEQSILGPVMLSHNKKEEGQERILHFINAHFGPVADFDAFVYLSQLD
                     QAEAIKFGIEHWRARKYWTAGTLYWQFNDSWPVFSWSSVDYFKRPKALYYYTRRFYAD
                     VLPIIRYERSDGAVSVTVVNDRHEDALMDIVLEAWDTGGKKLWDKKYTAVRVPADSVA
                     AIDIVTASEIPTVSLADAVIHVRVLCGEEHYDNYTLFGNFKDMHIMDPGVSYVREGDD
                     LVFRCIRPAFGVHIVADEECSLSDDFFTLVPSAVTRVECSSDMIHVASLYDYLTPAGG
                     NVGAEAGTRA"
BASE COUNT          464 a          768 c          615 g          518 t
ORIGIN      
        1 cccgaacctc ttcgtagttc agcgccccgt tttgcacaac tctgaccagc gaacgcgtat
       61 agtagttgac ccccaggaag tcgatcccat gtgagatcct ctgcaagtcg ccatccctga
      121 tgaagtcaag atcccccacg agctttcgaa gcacttccct ggtgtcctcc gggtatgagc
      181 ctttgagcac gggatccagg aaccatctgt tggcaattcc gtctgacctg gagacggctt
      241 ccaggtctgc cctcgaccgc gtgaagggga ccgcaggcgt cagattgagc gtgattccca
      301 ccttgccatc cctgaggccg caattcctga acgcctccac ggcggcacca tgggagagca
      361 ggatgtggtg agccgctatg agagcctctc tcatgtcctg gtggcccggc gcatgaatgc
      421 ccatgccata tcccagcatg gacgcgcaga atggctcgtt atgagtgatc cacagcttca
      481 cggagtcgcc cagtttctcg aataccttcg cagcgtattc cgcgaaccat gcgaccgact
      541 ccctgttcag ccatcctccc cgctcgtgcg cccagttcgg cagatcccag tgataaagag
      601 tgacgacagg atcgatgccg ctctcctgca gttcgtggat gagactcctg tagaactcca
      661 taccctttgg gttgtacgtc ccataggccg ggaatatcct tggccatgca atggagaacc
      721 tgtacgagtc gacgcccatc tccttcatga gcctgacatc ttccctgtac ctgtgatagt
      781 tatcgcaggc aacatcgccg gtctctcctc catggacctt gccaggcgtc ctgcagaacg
      841 tgtcccagat ggaaggggtc ctgccgtcct ctgcggcagc tccttcaact tgataggccg
      901 ccgtcgccac gccaaacctg aaatccttcg cgaatgtcgc cacatcacac ctcgctgctg
      961 ttgtcgtccc tcgaaaccac cttcacgcgc gcgttccagc ctctgctccc acgttcccgc
     1021 cggccggggt caggtagtcg tacaggcttg ccacgtggat catgtccgag gagcattcta
     1081 cacgtgtgac cgctgatggc accagggtga agaagtcatc cgaaagactg cactcttcat
     1141 cggccactat gtgtacgccg aaggccggcc ttatacaccg gaacaccagg tcgtcgcctt
     1201 ccctgacgta cgacacccct gggtccatga tgtgcatgtc cttgaaattg ccaaacagag
     1261 tgtagttatc gtagtgctcc tcaccgcaca ggaccctcac atgtatcacc gcatctgcaa
     1321 gagacaccgt gggtatctcg ctcgccgtga cgatgtcgat cgcggcgacc gagtccgccg
     1381 gcacccggac tgccgtgtac ttcttgtccc aaagcttctt tcctcccgtg tcccaagctt
     1441 ccagcactat atccatgagc gcgtcttcat ggcggtcgtt caccacggtg acgctcacgg
     1501 caccgtccga acgttcgtag cggatgatgg gcaggacgtc tgcgtagaac ctcctcgtgt
     1561 agtagtacag ggccttcggc ctcttgaagt agtccaccga cgaccagctg aacactggcc
     1621 agctgtcgtt gaactgccag tacagcgtgc ctgcggtcca gtactttctc gccctccagt
     1681 gttcgatgcc gaacttgatg gcttcggcct ggtcaagctg cgagagatag acaaacgcgt
     1741 cgaagtccgc caccgggcca aagtgagcat taatgaagtg gaggatccgt tcctgccctt
     1801 cctccttctt gttgtggctc aacatgacgg ggccaaggat gctctgttcc tcgctcgtgg
     1861 caaagaagtc aatggtcctt gcatccggcg cggactcgaa cccgaattcg ctgatgaacc
     1921 gcccgtcctc gtccgcatag ctcctgtagt ccgcccatcc cgaccagacg ttccagacat
     1981 gacggtcgcc atatccggca gcgttcgcct tatcgccccc atacgggctt gaaggccagt
     2041 acggcctcga cgggtcctcc cgggcacaga tcaagggaaa gtcgtgcagg tacagcctgt
     2101 tgcccaggta ctcaccatcc accttgtgtg ccatcatggg ccattcgtcg aagcccatgt
     2161 tgttctcgtt gtttccacac cacaggacga tgctcgcgtg atgacgcagc tgtctgacgg
     2221 tctcccggac ctccgtgttg cacagacgtc tgaaccatgc gaggtggtcg gggtattcgg
     2281 cacacgcgaa catgaagtcc tgccacacca tgatccccag ttcatcacac aaggcataga
     2341 actgttcgct ctcgtagatg ccgcc
//
LOCUS       DATMZD010000125         2975 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_124144,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000125 DATMZD010000000
VERSION     DATMZD010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124144"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VN478_02620"
     CDS             complement(<1..219)
                     /locus_tag="VN478_02620"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HWQ21607.1"
                     /translation="MGTQETFFERVWGVAARVPRGKVTTYGAIARALGSPGAARTVGW
                     AMRAAPSASRLPCHRVVNAAGDMSPEDAF"
     gene            complement(194..1432)
                     /locus_tag="VN478_02625"
     CDS             complement(194..1432)
                     /locus_tag="VN478_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWQ21608.1"
                     /translation="MAADAPAGPWLFMHPIYTVQVNPVQGTGSEEHDMQETTQYIDLF
                     DAAIRALFSDAVRITLRDPSIAVFFMRMRHAQHVAERRRARFAREGVHVPPFMILSVT
                     GRCNLACAGCYAQAQGRQEEAEMAPADLLSVVRQGSDLGVGIILLAGGEPMTRADEIL
                     TIAHEVPDVVFPVFTNGTLLVESLVSRIRGQRNIIPVISLEGHTAETDARRGEGVAGQ
                     AKAAIARMRKAGVFFGTSLTVTRLNIDTVLDEEYLRGLAGLGCRLFFFVEYVPVREGT
                     ELLVLDGAQRARLAATVASLQHRLRGLFIAFPGDEEALGGCLAAGRGFIHVSPSGRVE
                     PCPFAPYSDTSVRDVPLRDALASPLLAAIRENHDKLTETSGGCALWSQREWVESLLEG
                     CDGAETVRPGRPHGDARDIL"
     gene            complement(1581..2393)
                     /locus_tag="VN478_02630"
     CDS             complement(1581..2393)
                     /locus_tag="VN478_02630"
                     /inference="COORDINATES: protein motif:HMM:NF025115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HWQ21609.1"
                     /translation="MRNARTVLLLMIPAILLASGGCRPKVVSTPAVGLPTVVVQTVRE
                     EDAMLRYSITVSYPQLLGTLSSAVVEKVNKAIAAMVLPSIAGLKQNAADDAAWAAKNP
                     SEADQLTADQGSFLSAEYEIPYLTSELVSVRIRFATYTSGAAHGMTSTRVLNMRLNDG
                     TTIGTEGLFTDAKQGLQWLSQYCVTDLKRQYGTDYEAMTEFVEGGTAPVADNFTSVSL
                     EPGGLVVSFDPYQVGPYAAGPKEVHIPASEVQSMLKYSLSPDAFSLVREPME"
     gene            complement(2607..>2975)
                     /locus_tag="VN478_02635"
     CDS             complement(2607..>2975)
                     /locus_tag="VN478_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21610.1"
                     /translation="QENWFVVDVATALYFGMWSEHVGQGNRGWYRDLLDAFTNGYTRE
                     NALGSEWLGDRLQLSLKCQELWCYAAINDDWHLGQGGCWADVPAGWKRVLERYRRNIE
                     EGIPVADGGFNIWKVEAGVE"
BASE COUNT          558 a         1004 c          865 g          548 t
ORIGIN      
        1 aaacgcgtcc tcaggagaca tgtcgcccgc agcgttgaca acgcggtgac acggcagcct
       61 ggatgcggac ggtgccgccc gcatcgccca gccgactgtc ctggcagcac ccggactgcc
      121 cagcgctcgc gctattgctc cgtacgtcgt caccttgccg cgcggcaccc tcgccgcgac
      181 gccccagacc cgctcaaaga atgtctcttg cgtccccatg cggtcgtccc ggccggacgg
      241 tctccgcccc atcgcagccc tcaagcagcg actcgaccca ctcgcgctgc gaccagagtg
      301 cacagcctcc gctcgtctcg gtcagcttgt catggttctc gcggatggcc gcaagcaggg
      361 gcgacgccag agcgtcgcgc aacggcacgt ctcggacgct cgtgtcggaa tatggagcga
      421 acggacaagg ctccacccgt ccggacgggc tgacgtggat gaacccacgc cctgccgcga
      481 ggcagccacc gagggcctct tcgtcgccgg gaaatgcgat gaacaacccg cgcaagcgat
      541 gctgcagtga tgcgaccgtc gcggccagtc gtgcccgctg ggcgccgtcc aggacgagca
      601 actcggtgcc ctctcgcact gggacgtatt cgacgaagaa gaacagcctg catccgaggc
      661 ccgcaagacc gcgcaggtac tcctcatcaa ggaccgtgtc gatgttcagc cgagtgaccg
      721 tcaatgacgt cccgaagaag acgccggcct tgcgcatccg cgcaatggcc gcctttgcct
      781 gcccggccac gccctccccc ctccgcgcgt cagtctccgc tgtgtgtccc tccaggctga
      841 tgaccgggat gatgttccgc tggccacgga tacggctcac cagcgattcg accaggagcg
      901 taccgttcgt gaacacaggg aagacaacgt ccggcacttc gtgtgcgatc gtgaggatct
      961 cgtcggccct cgtcatcggc tcaccgcctg cgagcagaat gatcccgacg cccagatcgc
     1021 tcccctgacg gacgacgctc agcaggtcgg caggagccat ctcggcctcc tcctggcgcc
     1081 cctgagcctg ggcgtagcag cccgcacacg ccaggttgca ccgaccggtc acactgagga
     1141 tcatgaacgg cggcacgtgc acgccttctc tcgcgaacct ggcccggcgg cgctccgcta
     1201 catgctgggc gtgccgcatc cgcatgaaga acacggcaat ggacggatct cgcagtgtga
     1261 tgcgcacggc gtcggaaaac agggccctga tggcagcgtc aaacaagtct atgtactggg
     1321 tcgtctcttg catatcgtgc tcctcggaac ccgtgccctg cacgggattg acctgcacag
     1381 tatagatggg gtgcatgaat agccaaggcc ctgccggcgc gtctgccgcc atgcccacac
     1441 tcatttcctg gttgagaagt tgggccgcgt atagttggtc aggcaattgc catgtgtggc
     1501 gcattgacgc agtcaaggag ctactacgca ggtcatcaac gtcgtctgga cgcaccagga
     1561 agcagctggc cgcccggacc tcactccatc ggttctcgaa ccagactgaa ggcatccggc
     1621 gacagcgagt acttcagcat cgactggacc tcacttgctg gaatgtgcac ttctttcgga
     1681 ccggcagcgt atggaccgac ctggtaggga tcgaatgaga cgaccagccc gcccggctcg
     1741 agcgacacgc tcgtgaagtt gtcggccaca ggtgctgttc caccttccac gaactccgtc
     1801 atcgcctcgt agtctgttcc atactggcgc ttcaggtctg tgacgcagta ctgcgacagc
     1861 cactgcaaac cttgcttcgc gtcggtgaac aacccttcag tgcctatggt tgtcccatca
     1921 ttgagcctca tgttgagaac acgagtagac gtcatgccgt gcgctgcccc tgaggtgtat
     1981 gtcgcgaatc ggatacggac gctgacaagt tcgctggtca gataggggat ctcatactct
     2041 gcgctcagga aactgccctg gtcggcagtc agctggtcag cctcggatgg gttcttggca
     2101 gcccaggctg catcgtcagc ggcattctgc ttcagtcctg cgatactcgg cagcaccatg
     2161 gcggcaatgg ccttgttgac cttctccacc acggccgagg acagcgtgcc gagcagctgc
     2221 gggtagctga cagtgatgga gtaacgaagc attgcgtcct cctcgcggac tgtctggacg
     2281 acgacagtcg gcagtccgac agcaggcgtg ctcaccacct tcggccggca tcccccggaa
     2341 gcgagaagaa ttgccgggat catcagcagc agtacagtcc gcgcattcct cacacgatcc
     2401 tcccgtgccg tttcttcacg tacgtcccga cactgttcat aagcatagcg ctcgggatgt
     2461 ggaacgccag cgtccgaagc tgtgctcggc tgccagccgt cagttgtctc tccgaggcga
     2521 agacacgatg ctcgacattg tgtcctgtca ccaggctcag tcatccgctc ggaagagcgg
     2581 ggagtagcaa cgtcgcgaca tcggacctat tcgacgcctg cctccacttt ccagatattg
     2641 aagcctccgt cggccaccgg aatgccctct tcgatgttgc gacggtaccg ctccagcact
     2701 cgcttccatc cggcgggaac atccgcccag cagccaccct ggcccagatg ccaatcgtcg
     2761 ttgatggcgg cgtagcacca cagctcctgg catttcaagg acagctgcag ccggtcgcct
     2821 agccattctg agccaagcgc attctcccgc gtgtagccgt tcgtgaacgc atcaaggagg
     2881 tcccgatacc acccgcggtt gccctgcccc acgtgctcgg accacatgcc aaagtagaga
     2941 gcggttgcga cgtcgactac gaaccagttc tcttg
//
LOCUS       DATMZD010000126         2186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_53963,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000126 DATMZD010000000
VERSION     DATMZD010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53963"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VN478_02640"
     CDS             complement(<1..258)
                     /locus_tag="VN478_02640"
                     /inference="COORDINATES: protein motif:HMM:NF015043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HWQ21611.1"
                     /translation="MTSGIAPRCGIGFASLLDEVSRAPLTFTGTVPLILAGTMYRIGP
                     GRFQIGDHAVGHWLDGFALVSSITLSTGGATFSNRFLGSSWY"
     gene            complement(320..410)
                     /locus_tag="VN478_02645"
     tRNA            complement(320..410)
                     /locus_tag="VN478_02645"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(374..376),aa:Ser,seq:cga)
     gene            complement(397..867)
                     /locus_tag="VN478_02650"
     CDS             complement(397..867)
                     /locus_tag="VN478_02650"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015740084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HWQ21612.1"
                     /translation="MSLTDIECMRFALDEARRALDEGEMPVGCVIAKGGELVASAHNT
                     RERAKDPTAHAEILALRSCAERLQDWRLDGCVLYVTLEPCPMCAAATALARIQRVVFA
                     AYDFDNGAVGSNGNMLTRPIFGYAPEVVSGLLRADSEAVLQQFFAHIRSKRRGG"
     gene            complement(876..952)
                     /locus_tag="VN478_02655"
     tRNA            complement(876..952)
                     /locus_tag="VN478_02655"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(916..918),aa:Arg,seq:acg)
     gene            1061..>2186
                     /locus_tag="VN478_02660"
     CDS             1061..>2186
                     /locus_tag="VN478_02660"
                     /inference="COORDINATES: protein motif:HMM:NF015451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HWQ21613.1"
                     /translation="METRADVCIIGGGAAGMLAALSAARRIKTSGRDGAVLIVERNRV
                     LGMKLSITGKGRANVTNARSMDEFVQAFGPAGRFLYPAFESFFSDDLAALLGEAGLRL
                     KTERGGRMFPVTDRAQDVVQALGLLLVRDGVDVRYSARARSLECDSGDWTIGLESGGP
                     LRCRAVVVSSGGKSYPGTGSTGDGYGLLEHVGHHPGPLFPGLVSLHCPERWLNQLAGT
                     SLEGVAVEAWVGSRLLGRSQGEIMITDRGVGGPAVLSLSLAVTPALAEGQEVTLSLDL
                     RPERTSDQELRDIGACGSADEILVHVSRFLPKRTAIELTRVWGIDTKGRTPLPHRTLA
                     AIGASIKGIRLRCTGADPLSSAIITQGGIPLREVDPRSMGS"
BASE COUNT          451 a          639 c          675 g          421 t
ORIGIN      
        1 ataccaggaa cttccaagaa agcggttact gaacgtagcg ccgccggtcg agagtgtaat
       61 cgacgacaca agtgcgaagc cgtccagcca gtggcctact gcatgatcgc cgatctggaa
      121 cctgcccggt ccgatccggt acatggtacc ggcgaggatc agagggacag tcccagtgaa
      181 ggtgaggggt gcacgggaga cttcatcgag gagtgaggcg aaaccgatac cgcatcttgg
      241 cgcaattccc gaggtcactg ccacacctca cggccagaga cacgacgact cgctgatcgc
      301 tccagcatct gaaagatgct ggcggagagg gtgggatttg aacccacgaa cgagggaacc
      361 ccgtcacctg ctttcgaggc aggcgctttc aaccgctcag ccacctctcc gtttggacct
      421 gatatgtgca aagaactgct gcagaacagc ctccgagtca gcgcgcagca aaccggatac
      481 cacttcagga gcgtatccga agatgggcct cgtaagcata ttgccattgc tgcccacagc
      541 tccgttgtcg aagtcgtacg cggcgaacac gactcgctgt atcctcgcga gagctgtcgc
      601 agcagcacac atcggacagg gctcgagcgt aacgtacaga acgcagccgt ccagtcgcca
      661 atcctgcagg cgctctgcac acgaacgcag agcaagtatc tcggcgtgcg ccgtcgggtc
      721 cttggcacgc tctcgcgtgt tgtgtgcgga agcaaccaac tcgccaccct tcgcgatgac
      781 acagcccaca ggcatctctc cttcatcaag agcacgcctc gcctcatcaa gtgcaaagcg
      841 catgcattca atgtcggtaa gactcatgtc aggactggtg cgcccgacag gaatcgaacc
      901 tgtaacctac ggttccgtag accgtcactc tatccagttg agctacgggc gcacaacgat
      961 gattgtattc catttccccc ggttggcaat aacgacagtg aggattgctg ggccacgtgg
     1021 cagcagggat gcacacaaat ctgttataat ctagccggcc atggaaacaa gagctgacgt
     1081 ctgtatcata ggtggcggtg ccgccggaat gctagccgcg ttgtctgctg caagacgcat
     1141 caagacctca gggcgcgatg gtgcagtcct catagtcgaa cgcaacaggg tgctgggcat
     1201 gaagctgtcc atcaccggaa agggcagggc aaacgtcacg aatgcgcgtt cgatggatga
     1261 gtttgtccag gccttcggcc ctgcggggcg gttcctctac cccgctttcg agtccttctt
     1321 ctcggatgat ctggctgccc ttctgggcga ggccggtctt cggctcaaga ccgagcgagg
     1381 ggggcgcatg ttccctgtga cggacagggc tcaggatgtt gtccaggcac tcgggctgct
     1441 acttgtgaga gatggcgtcg acgtgcgcta ctcggcccgc gcgagaagcc tcgaatgcga
     1501 ttccggcgac tggaccatcg gactggagtc tggcgggccg ctgcgatgtc gagccgtggt
     1561 ggtgtcgtca ggtggaaagt cctatcccgg cactgggtcg acgggagatg gctatggcct
     1621 gctcgagcac gtaggacatc atcccggtcc ccttttcccc ggcctggtgt ccctgcattg
     1681 tccggagagg tggctgaacc agcttgcggg gacctcgctc gaaggtgtcg cagtggaggc
     1741 atgggttggt tcacggctcc tgggtcgaag ccagggagag atcatgataa cggaccgtgg
     1801 agttggcgga ccagccgttc tctcgctgag tctggccgtg acacccgcat tggctgaggg
     1861 acaggaggtc actctgtcac tggatctgcg gccggagagg acatccgatc aggagcttcg
     1921 cgacattggg gcttgcggga gtgctgatga gatcctcgtg catgtctcga gattccttcc
     1981 caaacgtacc gctatcgagc tcacacgcgt gtggggaatc gacacgaaag gcaggacacc
     2041 tctgccccac aggaccctgg ctgctatcgg cgcatcgata aagggcattc ggctgagatg
     2101 caccggggcc gacccgctgt cctccgcgat catcacgcag gggggcattc ctcttcgaga
     2161 ggttgacccg cgatccatgg gatcga
//
LOCUS       DATMZD010000127         3043 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_13910,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000127 DATMZD010000000
VERSION     DATMZD010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13910"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2916
                     /locus_tag="VN478_02665"
     CDS             <1..2916
                     /locus_tag="VN478_02665"
                     /inference="COORDINATES: protein motif:HMM:NF024487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HWQ21614.1"
                     /translation="LWYRAVRCVLDEPDEGVDTTLLLTGQWAHTPLWSFATIAATSCM
                     RSMDGRISTTSTSLVTVQPDILVEATTLAGCFTPGIEKPNAKLALVGRYRPRVVRWQI
                     LAGTIANALFDSLAAGMDIDPAAFVEAQLRTQGLALLTLSDDDVDMIRASVLGHLGGI
                     RRAAERVRTGGTAAMEVSLASSRYGLQGRLDALVTGKDQRAFSVVELKNGAVPHAAPG
                     QQDDGSSVRSEHAAQVWCYEFLVQSALGAQPRAADVVYLADEAHPWRGVGSREQDRIR
                     LLQTRNELVALDHAVAFEGDTSVFRHLTRNGFGPTGYFDNETVSSLASALDGATEAER
                     AYFLAFSSFLGRERWTSDLGTADDNGHGSGFAALWLMDLAQKQQRLAVLPYLRITGRD
                     PDSGAVHIVQRPIRQDGTSFREGDGVILYRHEGANARPLQGPLFKGTITAIEHRLGEA
                     TVVLYDRHAVLDTSAFWALEADASGGLITSQYAGLLHFLQVPAARRALLMGMEAPRSL
                     DPAVLDVHPARDLTPHQRELLGEALAAQDYFLMQGPPGSGKTQMMMVEMVRHLVHHTD
                     EAVLLMAYTNRAVGEMCHALSGDTDPALHAYLVFGRDAGMDDQIQGNSFVSSVEEHGA
                     TEGRALASRSRVFVATVAMCTQHPEILQELKRRRVRVTTIVDEAAQLLEPQVIGILAD
                     VDRAILIGDERQLSAVVQQPAQLAAVTDPVLLDAGFRDLRMSLFERLLRRCEERGWSH
                     AYGMLTDQARMHQDIVAFPDREFYGSRLQPLYDWQSSIEPAFVPDVNNPLECLLAARR
                     VLFIPSSEQDGVSRVHEQEARRVALLVQAFAAAYRRQDPTFDPAASIGVITPFRAQAA
                     CIYRALPAELQGVTVDTVERYQGGERDIIIVSFAVHSVSQMPSITAPSWDGSVDRKLN
                     VMLTRARKHLVLLGDPRVLEDSRLEDGSPSHHARLLWYLREQGDWMEEPEKLLDVEG"
BASE COUNT          504 a          950 c         1054 g          535 t
ORIGIN      
        1 ttgtggtacc gtgccgtgcg gtgcgtcctg gacgagccgg acgagggagt cgacacgacc
       61 ctgctgctga cggggcagtg ggcgcatacg ccgctgtggt cgtttgcgac gatcgccgcg
      121 acttcctgca tgcgctcgat ggacgggcgc atcagtacga cgagcacaag cctggtgacg
      181 gtgcagcccg acatcctcgt ggaggcgact acgcttgcgg gctgtttcac tcccgggatc
      241 gagaagccaa atgccaagct ggcgctggtg gggagatacc gcccgcgtgt cgtgcgctgg
      301 cagatcctcg caggcaccat cgccaacgcg ctgttcgaca gtctggccgc cggcatggac
      361 atcgaccctg ccgcattcgt cgaggcacag ctgcgcacac agggattggc cctgctgacc
      421 ctctcggacg atgacgtgga catgatccgc gcgagcgtgc tgggacacct gggtgggata
      481 cgccgcgcgg ccgaacgcgt ccgcacgggt ggcacggccg cgatggaagt atctctggcc
      541 tccagccgct acggcctgca gggccgactg gacgccttgg tgaccggcaa ggaccagcgg
      601 gcgttcagcg tcgtggagct gaagaacggc gcggtgccac atgccgctcc gggacagcag
      661 gacgacggca gcagcgtgcg ttccgagcat gccgcacaag tctggtgcta tgagttcctt
      721 gtgcagtcgg cactgggcgc ccagccgcgg gccgcagacg tcgtgtacct ggcggacgag
      781 gcacacccgt ggcgtggagt gggttcgcgg gagcaggacc gtatccggct gctgcagacg
      841 cgcaatgaac tggtggccct ggaccacgca gtcgccttcg agggagacac gtcggtcttc
      901 cggcacttga ccaggaatgg gtttggaccc accgggtact tcgacaacga gacggtcagc
      961 tcgctggctt ccgccctcga cggggcgaca gaggcggagc gggcgtactt cctcgcgttc
     1021 tcgtcgttcc tcgggcgcga gcgctggacg tcggacctgg gcacggcgga cgacaacggt
     1081 catggcagcg gttttgccgc actgtggctg atggacctgg cacagaagca acagcgtctg
     1141 gcagtcctgc cgtacctgcg catcaccggc agggatccgg acagtggggc cgtgcacatt
     1201 gtccagcgtc ccatacgcca ggacgggacg agcttccgcg aaggcgacgg cgtcatcctg
     1261 taccgccacg agggagcgaa tgcccggcca ctgcagggac cactgttcaa agggactatc
     1321 acggcgatcg aacaccgcct gggcgaggcg accgtcgtgt tgtatgaccg gcatgccgtt
     1381 ctggacacgt ccgcgttctg ggcgctggag gcggatgctt cgggcggcct catcacgtcg
     1441 cagtacgccg gtttgctgca cttcctgcag gtaccggctg cgaggcgtgc cctgctgatg
     1501 ggtatggaag cgcctcggtc gctggacccg gcggtgctcg acgtacatcc cgcccgggat
     1561 ctgacgccac accagcgtga gctgctggga gaggcgctgg ccgcgcagga ctacttcctg
     1621 atgcaggggc cgcccggctc gggcaagacg cagatgatga tggtggagat ggtccggcac
     1681 ctggtgcacc acacggacga ggcggtcctg ctgatggcgt acaccaaccg cgctgtgggg
     1741 gagatgtgcc atgccctgag cggtgacacg gatccggctc tgcacgccta cctcgtcttc
     1801 gggcgggatg ccggcatgga cgatcagata cagggcaaca gctttgtatc ttcagttgag
     1861 gagcacggcg ccaccgaggg acgggcgctg gcgtcgcgga gccgcgtgtt tgtggcgacc
     1921 gtcgccatgt gcacgcagca cccggagatc ctgcaggagc tgaagcggcg gagggtccgc
     1981 gtgacgacca tcgtggacga ggcagcgcag ctgctcgagc cgcaggtgat tgggatcctg
     2041 gcggatgtcg accgtgccat cctcatcggg gacgagcgcc agctgtcagc cgtcgtgcag
     2101 cagcctgcac agttggcagc tgtcaccgat ccagtgctgc tggacgccgg gttccgcgac
     2161 ctgcgcatgt cactgttcga gcgcctgctg cgccggtgcg aggaaagggg atggtcacat
     2221 gcgtatggca tgctgaccga ccaggcgcgc atgcatcagg acatcgtcgc gttccccgac
     2281 cgcgagttct acggtagtcg gctgcaacca ctgtatgact ggcagtcatc gatcgaacct
     2341 gcattcgtgc cggatgtgaa caacccgctg gaatgcctgc tggctgcccg gcgggtcctg
     2401 ttcatcccgt cgtctgaaca ggatggcgtg tcgcgcgtgc atgagcagga ggcccggcgc
     2461 gttgccctgc tggtgcaggc gtttgcagct gcctaccgca gacaggaccc gacgttcgat
     2521 ccagctgcat ccatcggcgt catcacgccc ttccgtgccc aggccgcgtg catctaccgt
     2581 gctctgcccg cagagctgca gggcgtcacc gtggacaccg tggagcgcta tcagggcggc
     2641 gagcgcgata tcatcatcgt gtcgttcgct gtccattctg tctcccagat gccgtccatc
     2701 accgctccct cctgggacgg gtcagtcgac cgcaagctca acgtcatgct gacgcgggcc
     2761 aggaagcacc ttgtgctcct cggagacccg cgcgtcctgg aggactcccg tctcgaggac
     2821 ggcagtccct cgcatcatgc ccgcctgctg tggtacctgc gggaacaagg agactggatg
     2881 gaggagccgg agaagctcct ggacgttgag ggttgagggt cgatgggtct atgaggtcag
     2941 ctggtaattg cgcgtccggt ggtacgtact ctcggctaga tcgggagtag cgcgtcggac
     3001 tgtagcactc ttggaaccac cctgatctgc agcactgttg cac
//
LOCUS       DATMZD010000128         3928 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_124191,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000128 DATMZD010000000
VERSION     DATMZD010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3928
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124191"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..884)
                     /locus_tag="VN478_02670"
     CDS             complement(<1..884)
                     /locus_tag="VN478_02670"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWQ21615.1"
                     /translation="MINVTRSELPALDEYVSYLAGIWKTHWLTNDGELVQELERRLAS
                     HLHVQNLAVVANGTLALQLAFKALKLTGEVVTTPFTFAATTNALLWEGLQPVFADIDP
                     ETYNIAASSVEERITPRTSAILAVHVYGNPCDVEALQDVATRNGLPIIYDAAHAFGVE
                     YRGKSVLEYGDLSTMSFHATKVFHTIEGGAAITRLPETLHEIQLLRNFGIVSEEEVVA
                     PGINAKMNEFEAAMGLCNLERVNDECTRRKSIYEKYKTALSSIPGVQFQRLVASRYNY
                     AYMPVRFASAAVRDAVYEG"
     gene            complement(942..2018)
                     /locus_tag="VN478_02675"
     CDS             complement(942..2018)
                     /locus_tag="VN478_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21616.1"
                     /translation="MKRTVTGLRLQDLRIRRSQYRAAADAVHVDRKRLIVFLVPGWDG
                     VNGGILSIVSLCAESRKLCANRNADVFLVSSPGDPPLMGYTKFANREPILDLTDVVQR
                     HRSLDYLLIHVPECYVAQFAASAGWRFRARLLRIRNVRFNVMLQNIDLLPDSQSLKRL
                     ASLGVLTITTAHEQYTSREMRQGLGFPLHHLSTFVSPGQYVRVPLTDKENLLVLSPDP
                     SDQRGRILGLLHERLPEMELVTVQGMKYEDYKSLLQRARWSLTFGEGLDGYFVETVFS
                     GGIAFAVYNSRFFTGDFHVLRTVYESWDELAGRICDDIGDLGVSSSYEAYQKAEYALC
                     AKHYDHSVYLDNLRKFYAGEYSYP"
     gene            complement(2305..2856)
                     /locus_tag="VN478_02680"
     CDS             complement(2305..2856)
                     /locus_tag="VN478_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWQ21617.1"
                     /translation="MAPEQVVVSEMDNRNLRVIRSSVVEFMKRSASTYAVTQGRLLDI
                     APQVHEGARPFFPEWIAVETLDIDPKSGCTYVGDICEHNSLLPDGGFDYVVCTEVLEH
                     TLRPWDAVAEIWRILKPAGLLFLSVPFNFRIHGPLPDCWHFTEHGLRALLSDRFLILS
                     LDAVETRDRPLMPIHYTVTARKT"
     gene            complement(2858..>3928)
                     /locus_tag="VN478_02685"
     CDS             complement(2858..>3928)
                     /locus_tag="VN478_02685"
                     /inference="COORDINATES: protein motif:HMM:NF014686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HWQ21618.1"
                     /translation="CSIDYVFPAHDDVVVALAQNAGRIRAGVVSSPPETCLVARSKSA
                     TYRLLADAIPVPELYACPETIDLFPVFVKPDKGQGSRDIQLVHDRDHLRQVVAEHPDC
                     IISEYLPGEEYTVDCFSDRQAGLLFCGGRQRIRIRSGISMDSRPVQDDAFGEYARRIS
                     EKLDFHGAWFFQVKRDRHGLCKLMEIAPRIAGTMALYRVLGVNFPLLSIYEQERIPLE
                     IRTNAVDVQIDRALVNRYKHDLSFGVVYVDLDDTLVVNGKANTDLIRLLYQCINAGKR
                     IVLLTRHAGDLDQCLRKHHLTGMFDDVIQLEQAASKADCIHESNAIMIDDSFSERKQV
                     SEKLGIPTFDCGMIEMLFDDRV"
BASE COUNT          877 a         1157 c         1046 g          848 t
ORIGIN      
        1 ccttcgtaca cggcatcgcg tactgctgcc gaggcgaagc gaaccggcat gtaggcatag
       61 ttgtatcgag aggccacgag cctctgaaac tggacaccgg ggatggagga caatgccgtc
      121 ttgtacttct cgtagattga cttgcggcgc gtacattcat cattcactct ctccagatta
      181 cacagcccca tggccgcttc gaactcgttc atcttggcat tgatgcctgg tgccaccact
      241 tcctcttctg atacaatacc gaagttacgg aggagctgaa tctcgtgtag cgtctctggg
      301 agtctcgtga tcgcggcgcc accttcaatg gtgtgaaaga ccttcgttgc gtggaaactc
      361 atcgtagaca ggtcaccata ctcaagcacc gacttgcccc gatactcgac gccaaaagca
      421 tgggcggcat cgtagatgat gggcagaccg tttcgcgttg ccacatcctg gagggcctca
      481 acgtcgcacg gattgccata gacatgcact gccagaatcg cgctagtccg cggcgtgatt
      541 ctttcttcga cgctcgaggc agcgatgttg tatgtttctg gatcgatatc ggcgaatact
      601 ggctgcaggc cctcccacaa gagagcgttg gtcgttgcag cgaaagtgaa cggcgtcgta
      661 acgacctcgc ccgtcagctt cagtgccttg aaggctagtt ggagtgccag tgtgccgttg
      721 gcgacaacag cgaggttctg cacatgaagg tggctcgcca gtcgccgctc cagttcctgc
      781 accaactcac cgtcgttggt gagccaatga gtcttccaga tgcccgcgag ataagaaacg
      841 tactcatcta gagcaggcaa ctcagagcga gtgacattga tcaaagcacc ctcctcttcc
      901 tggcagcatt ttaccgtgag ccccagaacg tatcggccag ttcatggata cgagtactcg
      961 ccagcgtaga acttgcgcag gttgtcaagg tacacgctat ggtcgtagtg cttcgcacac
     1021 agagcatact ccgccttctg gtacgcttcg taactggaac tcacgcccaa atcgccaatg
     1081 tcgtcacaga tccggcctgc gagctcatcc cagctctcat agactgtgcg gaggacatga
     1141 aagtcacccg tgaagaacct tgagttgtag accgcaaagg caatcccgcc actgaagacg
     1201 gtctccacga aatacccatc aagcccttcc ccaaaagtga gagaccatct ggcacgctgg
     1261 agaagcgact tgtagtcctc gtacttcatc ccctgcacgg tgacaagttc catttcaggc
     1321 agccgctcat gcagaagtcc cagaatgcgt ccccgctggt cgcttggatc gggcgacagg
     1381 acaaggaggt tctccttgtc ggtcaggggc acgcgcacgt attgccccgg gctgacgaac
     1441 gtggacaagt gatgtagagg aaatcctagc ccctggcgca tctcccgtga ggtatactgc
     1501 tcgtgcgctg ttgtgatcgt cagtactccc agactcgcca ggcgcttcag gctctgggaa
     1561 tcaggcagaa gatcgatgtt ctggagcatg acattgaatc gcacgttgcg tattcgaagc
     1621 aacctggctc gaaagcgcca tccagcacta gccgcgaact gggccacgta gcattctgga
     1681 acgtgaatca gaaggtagtc cagcgagcga tgcctttgta ctacgtccgt caggtcaaga
     1741 atgggctcgc ggttggcaaa cttcgtgtag cccatcagcg ggggatcccc cggtgaactg
     1801 accaagaaca cgtcagcgtt tcgattggcg caaagcttcc tcgattcggc acacagagag
     1861 actattgaca ggataccgcc gttaacaccg tcccacccgg gcaccaggaa cacgatgagc
     1921 cgcttcctgt caacatgcac agcgtctgct gcagcgcgat actgggaacg tcgtattctc
     1981 aggtcctgca ggcgcaggcc cgttaccgtt cttttcaaca accgatccat gccgggacga
     2041 gatggtgcgt tatctagcca gcccaacaat gcagtcctca gaccaaacat gtatactctg
     2101 ccagtgcctg tcgaacgcaa cagatggccc tattctctat gccgccgtga ctgcagcttg
     2161 tgttccagct caccgccccc atgccgctgt cgctaccgat gtcgggatgc ccaacacgca
     2221 actggctgcg agacatcggt ccacttccag cgactgccag ctcacaggag tggtgtcccg
     2281 tattccaaaa gtcctccttg gcaatcatgt tttcctcgcc gtcacagtat agtgaatcgg
     2341 catcaacggc ctatcccggg tctcaactgc atcgaggctc aagatgagaa acctgtcact
     2401 cagcaaggcg cgcaggccat gctccgtgaa gtgccagcag tcaggtagag ggccgtgaat
     2461 cctgaagttg aagggaacgc tgagaaacag cagacccgct ggcttcagaa tcctccagat
     2521 ttctgccaca gcatcccagg gtcggagcgt atgctctagc acctcagtgc agactacgta
     2581 gtcaaatcct ccgtcgggca agaggctgtt gtgctcgcag atatcgccaa cgtacgtgca
     2641 ccccgacttc ggatcgatgt caagggtttc gacagctatc cactccggga agaacggcct
     2701 ggctccctca tgcacctgag gcgcgatgtc caggagtcga ccctgtgtca cggcgtaggt
     2761 ggaagcagag cgcttcatga attcgactac actcgaccgg atcacccgca ggttgcgatt
     2821 gtccatctcg cttactacga cctgttctgg cgccatttca gactcgatcg tcgaatagca
     2881 tctcgatcat gccacaatcg aatgtcggaa tccccagctt ctcactgacc tgcttgcgct
     2941 cgctgaagct gtcgtctatc atgattgcat tactctcgtg aatacagtct gccttgcttg
     3001 ccgcttgctc cagttgaatg acatcgtcga acatgcccgt cagatgatgc ttccgcaggc
     3061 attggtcaag atcacccgcg tgcctagtga ggaggacgat ccgctttcca gcgttgatac
     3121 attgatacag gagacggatc aggtcagtat ttgccttgcc gtttacaacg agcgtgtcgt
     3181 ccaggtcaac atacacgact ccaaatgaca gatcgtgctt gtagcggttc accagtgcac
     3241 ggtcgatctg cacatctact gcgttggttc gaatctcgag cggaatccgc tcctgctcgt
     3301 agatactcaa gagtggaaaa ttgacaccca gcacccggta taaggccatg gttccggcga
     3361 tccggggggc tatttccata agcttgcaga gaccatgacg gtctctcttc acctggaaga
     3421 accatgcccc gtggaaatcc agcttctccg agattcgtcg agcgtactca ccaaatgcgt
     3481 catcctgaac aggcctgctg tccatggaga tcccgcttcg gatccggatc ctttgtctgc
     3541 caccacagaa caacagcccc gcctgtcgat ctgagaagca gtcgaccgtg tactcctcgc
     3601 ccggcagata ctctgagatg atgcagtctg gatgttctgc gacgacctgg cggagatggt
     3661 ctctgtcatg gacaagttgt atgtcgcggg acccctgccc cttgtcaggc ttcacaaaca
     3721 cggggaacag gtcaatcgtc tccgggcatg cgtacaactc aggcacagga atggcgtcgg
     3781 cgagcaagcg gtatgtcgca gacttggaac gcgcgaccag gcaggtttcc gggggcgaag
     3841 acacgacgcc tgcgcgtatt ctaccggcgt tctgggccaa tgcgacaacc acatcgtcat
     3901 gcgcaggaaa gacgtagtca atcgaaca
//
LOCUS       DATMZD010000129         2109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_3601,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000129 DATMZD010000000
VERSION     DATMZD010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3601"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..1332)
                     /locus_tag="VN478_02690"
     CDS             complement(79..1332)
                     /locus_tag="VN478_02690"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HWQ21619.1"
                     /translation="MRRVAVVGAQWGDEGKGKVVNYFAREFEWIVRFAGGANAGHTIY
                     VGDKKYVNHLLPCILPDGPGRGFLGAGMVVDLEQLVNELEVLAKDFPGVGAKFAIDPE
                     VFLVLPWHKQQDQLLEAMRAEPIGTTGRGIGPAYTDKASREGIKLCHLFDQKMLMERL
                     ENSWVIKQNLYRGQLSMPPAELAALLVRQRDQLLRLGVEVTGAVDMAHVFRSTSVLFE
                     GAQGVLLDLDFGTYPFVTSGSCMAHGVSSIGFSTFELDDVAGVLKAYTTRVGEGPFPS
                     EDNSDVAQGIRERGKEYGATTGRPRRVGWLDLPALRYAAARSGLTSLVITKADVLNGL
                     KTVRVCTGYRIDGVVRDTPSTSHDFFVAEPVYVELPGWRTTDDVNFLKYMTFIEEQVS
                     VPITYISYGPHTEEMETKSRLIMNI"
     gene            complement(1329..>2109)
                     /locus_tag="VN478_02695"
     CDS             complement(1329..>2109)
                     /locus_tag="VN478_02695"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HWQ21620.1"
                     /translation="ISGAVGNYANIDPRVEDVALRSLGLRRAKCSTQIVPRDQHAAFM
                     AALALAGAQVERMATEVRLLQRTEVREAQEPFKVGQRGSSAMPHKQNPIVCERLCGMA
                     RLLRGYMTAAFEDVALWQERDISHSSVERVILPDGVMTTYYMLDRAIWLMKGLVVDAQ
                     AMQAAFAASHGLVYSQRVMLALVRGGMSREDAYALVQRLSLTAWEGGADFHQLCLSEP
                     HITSLIAPEDLDTLFDPQYYLRNIGPIYARFDLDNTREERS"
BASE COUNT          375 a          674 c          659 g          401 t
ORIGIN      
        1 gtatatctct gctgttgttt tgtccatcac tctgcgatgg gacgcaggcg ctgcactcgc
       61 ggaagtagca ggaagggctc agatattcat gatgagccgg ctcttggtct ccatctcctc
      121 tgtgtggggg ccatacgaga tgtaagtgat gggaacgctc acctgttctt cgatgaatgt
      181 catgtacttg aggaagttga cgtcatccgt cgtgcgccag ccaggcagct ccacgtagac
      241 tggttcggcg acgaagaagt cgtgggaggt cgaaggagtg tcgcgcacga cgccgtcgat
      301 gcggtagccg gtgcagaccc gcacagtctt cagcccgttg aggacgtcgg ccttggtgat
      361 caccaggctg gtgaggcccg accgggctgc ggcatatcgc aacgccggca ggtccagcca
      421 tccgacgcgg cgcggacggc cggtcgtggc gccatactcc tttccccgct cgcggatgcc
      481 ttgtgccacg tctgagttgt cttcactggg gaacggaccc tctcccacgc gggtcgtgta
      541 cgccttgagg actccggcga cgtcgtccag ctcgaatgtc gagaacccga tagaagagac
      601 gccgtgagcc atgcaggatc ccgacgtgac aaaggggtac gtcccgaagt ccaggtccag
      661 cagcaccccc tgagctccct cgaacaggac cgacgtactg cgaaagacgt gggccatgtc
      721 cacggctccc gttacctcga ctcccagccg caacagctgg tcccgctgac gcacaaggag
      781 cgcggccaac tctgcgggcg gcatggagag ctggcctcga tagaggttct gcttgatgac
      841 ccatgagttt tccaggcgtt ccatcagcat cttctggtcg aacaggtgac acagcttgat
      901 cccttcgcgg gacgccttgt cggtataggc cggtccgatg ccgcgcccgg tcgtcccgat
      961 gggttccgcg cgcatcgcct ccagcagctg gtcctgctgc ttgtgccagg gcaggaccag
     1021 gaagacttcc gggtcgatgg cgaacttggc cccgacgccg gggaagtcct ttgccagcac
     1081 ttccagttcg ttcaccagct gctccaggtc gacgaccatg ccggcgccca ggaaaccgcg
     1141 acccggtccg tcaggcagga tgcatggcag caggtgattg acgtacttct tgtcccccac
     1201 gtagatggtg tggcccgcat tggcccctcc cgcgaagcgg acgatccact cgaactccct
     1261 ggcgaaatag ttgacgacct tgcccttgcc ctcgtcgccc cactgggcgc ctaccactgc
     1321 gacacgtctc atgatcgttc ctccctcgtg ttgtccagat cgaaccgtgc atagatggga
     1381 ccgatgttcc tcaggtagta ttgcgggtca aagagcgtat ccaggtcctc tggagcgatc
     1441 agggacgtga tgtgaggctc cgacagacag agctggtgaa agtccgcacc tccctcccac
     1501 gccgtcagtg acaggcgctg gacaagagcg tatgcgtcct cacggctcat cccgccgcgc
     1561 accagcgcca gcatgacgcg ctgcgaatac acgaggccgt gggaggcggc aaacgccgcc
     1621 tgcatcgcct gggcgtcgac gacaagcccc ttcatcagcc agatggcccg atcgagcatg
     1681 tagtaggtgg tcatgacgcc gtccggcagg atgacccgtt ccaccgacga gtgtgagatg
     1741 tcgcgctcct gccagagcgc cacgtcctcg aaggctgccg tcatgtaccc tctcagcaga
     1801 cgcgccatgc cacagaggcg ctcacagacg atggggttct gtttgtgggg catggcggac
     1861 gagccccgct ggccgacctt gaatggttcc tgcgcttcgc gcacttcggt gcgctgcagc
     1921 agccggacct cggtcgccat gcgctcgacc tgcgcgcctg cgagggcgag agcagccatg
     1981 aacgcagcat gctggtcgcg cgggacgatc tgggtcgagc attttgccct ccgtagtccc
     2041 aggctcctga gggcgacgtc ttcgacacgg gggtcgatgt tggcgtagtt gccgacggca
     2101 cccgagatc
//
LOCUS       DATMZD010000130         7433 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_94215,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000130 DATMZD010000000
VERSION     DATMZD010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7433
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94215"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2081)
                     /locus_tag="VN478_02700"
     CDS             complement(<1..2081)
                     /locus_tag="VN478_02700"
                     /inference="COORDINATES: protein motif:HMM:NF037626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HWQ21621.1"
                     /translation="MQSGSFDLLSQQAQAGSPELFARFAQLLEEPGSIRVSTPSTGYR
                     QLLLAALIAGCPRPLVVVCPSVDRAEELSSMVRGLCGTVDTRQFVVLPELGVEIMEYT
                     QYSRPLSFSRARALDQIYAHAETVVFTTVQALIDPSIPAQDFAGLVSSLGTGDRVVPH
                     SLARRLIEMGYERVSSVTEMCQFSMRGNVFDLYSQVGEYPARIVLEGNVISRIKLFDP
                     VTQQSVEPVPSVGILPPYPVVLDADARRSFATLAAERWNAERGTREGSDEQEATFLED
                     LESFISVTNSKGVNYFLYQYMDPERMCGTLCRVLGPRAVALFVDVDPEAELTQIFDSA
                     RELHDRTVQAGFTPSSDALGHVELIARRAVSRFARSVSLGAASVPGALAPASRDLPPL
                     TLSASLSDYLLLHAPGETAIDVVSWNPDKARRLVTDTVEDPAVMARVSVVKGSLTAGF
                     AADGFVVLTDKELFPKTTMSPKKTTRFSQAISRLEDLHEGDYVVHRDYGIGVFRGITA
                     MTVDGITRDYISLEYKDHSLLHVPVERLGYLEKYVGDRAEITLDEPGSKKWKRARKEA
                     AEDARRIAHELLETAARRHAREGYMFKPNPELEVPVVDAFAYELTPDQSKAIADVLSD
                     MEAGKPMDRLVCGDVGFGKTEVAIRAAARAVANGKQVAVVAPTTILAMQHFRNLEARF
                     EPTGYKVALFS"
     gene            complement(2081..2680)
                     /gene="pth"
                     /locus_tag="VN478_02705"
     CDS             complement(2081..2680)
                     /gene="pth"
                     /locus_tag="VN478_02705"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HWQ21622.1"
                     /translation="MKTILIAGLGNPGSEYDETRHNVGFMALDAIAHSFKVRRFNHNR
                     LAHWAAADLALCDGPEPVRLVLVKPQTFMNASGVAIAPMMKERNILLENLIVIHDEMD
                     LPIGAIRISFNATAAGHKGVRSIAENCGGKGFVRVRLGIGKPDSADGVIDHVLSRFSR
                     SEQSSVIDAIAKTPTVIRTIICNGLMAAQDMYNRKVAKD"
     gene            2828..3136
                     /gene="rplU"
                     /locus_tag="VN478_02710"
     CDS             2828..3136
                     /gene="rplU"
                     /locus_tag="VN478_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004821439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HWQ21623.1"
                     /translation="MYAVIEAAGRQYRVELGETVKMEKIASEPGTPIVFDKVLVVTTD
                     AGTQIGKPYVLDAKVSGTILRTTRDRKVRVFTYKNKTTHKKLIGHRQTSTYVKITEIG
                     "
     gene            3152..3418
                     /gene="rpmA"
                     /locus_tag="VN478_02715"
     CDS             3152..3418
                     /gene="rpmA"
                     /locus_tag="VN478_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011612702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HWQ21624.1"
                     /translation="MAHKKAGGKSRNGRDSNPQYLGVKLYDGQVVQPGNIIVRQRGEH
                     FKAGSGVMMGRDYTLFAVVEGTVKFESLKTVKRVHVLPVPEQAN"
     gene            3460..4740
                     /gene="obgE"
                     /locus_tag="VN478_02720"
     CDS             3460..4740
                     /gene="obgE"
                     /locus_tag="VN478_02720"
                     /inference="COORDINATES: protein motif:HMM:NF008955.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HWQ21625.1"
                     /translation="MLDEVVVRVTSGHGGNGAMSFRREKYVEKGGPDGGDGGRGGDVI
                     FVATARYSSLQFFAKKRTFRAEAGEGGAGRNKHGSDGQDITILVPLGTVLTDVESEEV
                     LADLVDEGETVVVARGGSGGRGNAAFATSVDRAPRYSEEGIPSQERRIRLRLKILADV
                     GMVGFPNAGKSTLLSKLSAAHPKIADYPFTTLSPNLGVAFSPEGDRVTFADIPGLVEG
                     AADGRGLGNKFLAHIERSRVLLFLVDGSSADPDPIQAYHLLRRELEQYSEQLAGKPQL
                     VVVNKIDAISAQKRTGIRMRFVRQGIEPLMISALEGVGIDAIILRTLALVQSAPIPPR
                     EQVTRIYKLKPSDETFEITRLPDGMLSASGRYLDRIIHTTDFEKPFQVTLLDQRMRKI
                     GVEKALRKKGAHDGQNVMIGGRVFTLGEEIAKKR"
     gene            4737..5324
                     /gene="nadD"
                     /locus_tag="VN478_02725"
     CDS             4737..5324
                     /gene="nadD"
                     /locus_tag="VN478_02725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HWQ21626.1"
                     /translation="MKTVLYGGAFDPVHCGHVFVGHEALRLMAPCILVVIPTGLPNPD
                     FGKRLSGSDADRMAMLRLAFAGVPNVVISDIELDRGPQPSYFIDTIERLHPSLGDEKP
                     ALLLGEDQLLSFTEWHRYRDILDHVELWFVPRAGGHRAGGPRVAATALFDINPFDGLS
                     SSLIRDRLQQGLPVDGLVPPSVAAYIAEHALYKSQ"
     gene            5321..5929
                     /gene="yqeK"
                     /locus_tag="VN478_02730"
     CDS             5321..5929
                     /gene="yqeK"
                     /locus_tag="VN478_02730"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR00488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HWQ21627.1"
                     /translation="MTSRERARIVALVAQRVSQKRLEHTLRVETLAVALARFWGVSED
                     KVILASLLHDVARDEAETSLLELARGSSDPVVRGMAGTSAPVVLHAPVGALIARRNFG
                     VQDPQILQAIALHTTGGPHMDRLAMIVFLADYCESGRSFAGVDDVRLLLFASLEDAML
                     RALEQTLTYLRQNGRPIDPNTAEAAMAFAHPDRGDSQRPYRA"
     gene            5973..6047
                     /locus_tag="VN478_02735"
     tRNA            5973..6047
                     /locus_tag="VN478_02735"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6005..6007,aa:Gly,seq:ccc)
     gene            6050..6124
                     /locus_tag="VN478_02740"
     tRNA            6050..6124
                     /locus_tag="VN478_02740"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6082..6084,aa:Cys,seq:gca)
     gene            6146..6232
                     /locus_tag="VN478_02745"
     tRNA            6146..6232
                     /locus_tag="VN478_02745"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6180..6182,aa:Leu,seq:taa)
     gene            complement(6253..>7433)
                     /locus_tag="VN478_02750"
     CDS             complement(6253..>7433)
                     /locus_tag="VN478_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21628.1"
                     /translation="VMPVLAAEMSDLNPDRAWWKTKFPGANPFFHHCSFLQYYIARRN
                     GFPVGRIAAFVDRSYHEPMVHGEVGWIGLFESIDDEEVASALLGAATEDLQHSGTVKI
                     IGPARFNANGEDGLLVDGFDKHPMIMEPYHPPYYTRFLEKWGVKENDWFAFRMTHESA
                     SPYMERLDDMRKRGQDLEQRLARQGVAIRSVKMSDWADEIARVKTVYNVAWDTRVHPQ
                     FEQFSEEEFDYLAAGLKMIAIEDLVFVVEDMTKPNHPVIGMAVTLPDLNEVVEEYDGA
                     HPPYRPSRHVYGLSDLRRDTGVLKLLKSRVKHRDFKYARVFVLGTMRKKSGVDAMLYE
                     KTYLATIKMGVQLASGSQIADTNPEIFVPLSRMGNADITWRVYRHTRSLGNTGSDTEA
                     "
BASE COUNT         1525 a         2262 c         2198 g         1448 t
ORIGIN      
        1 ctggaaaaga gggcgacctt gtagccggtt ggttcgaatc gagcttccag attgcgaaag
       61 tgctgcatcg ccagaatggt cgtcggggcc acgacagcga cctgcttgcc gttggcaact
      121 gcccgggccg cagcccgtat cgcgacctcc gtcttcccga agccaacatc gccgcaaacg
      181 agccgatcca ttggcttgcc tgcctccatg tcgctcagca cgtcggcgat cgctttggac
      241 tggtctggtg tcagttcgta ggcaaatgcg tcgacgacag gcacttccag ctccgggttc
      301 ggcttgaaca tatagccctc gcgcgcatgt cgcctggccg ctgtctcaag cagctcgtgg
      361 gcgatgcgac gagcatcttc ggccgcttcc ttgcgtgcgc gcttccactt cttcgacccc
      421 ggttcgtcca gcgtgatctc cgcgcggtcc ccaacgtact tctcgagata tcccaggcgc
      481 tcgacaggca cgtggagcag tgaatggtcc ttgtactcca gagagatgta gtcccgggtg
      541 atcccgtcga ctgtcatggc ggtaatgccc ctgaataccc caatgccgta atcccggtga
      601 acgacgtagt cgccttcgtg caggtcctcc agacgcgaga tagcctgcga gaagcgagtc
      661 gtcttcttgg ggctcatcgt cgtcttcggg aacagctcct tgtccgtgag gaccacgaac
      721 ccatctgcag cgaacccggc agtgagcgac cccttcacca cagagactct cgccatgacg
      781 gccggatcct ccaccgtgtc cgtcaccagg cgccgcgcct tgtcgggatt ccatgacacg
      841 acgtcgatcg cggtctcgcc tggagcatgc agtagaaggt agtcgctcag ggaagccgag
      901 agcgtcaacg ggggcaaatc gcgcgaggcc ggcgcaagcg cccccggaac tgatgcggca
      961 ccgagcgaga ctgaccgcgc aaaacggctt acggcccgtc tcgcaatcag ttcgacatgc
     1021 ccaagggcat cggacgacgg cgtgaagcct gcctgaacgg tccgatcgtg gagttcccgc
     1081 gcggagtcga agatctgtgt cagttctgct tcagggtcga catctacaaa cagtgccacc
     1141 gcacggggtc cgagcacgcg gcacagggtc ccgcacatgc gctccggatc catgtactga
     1201 tacaggaagt agttcacacc cttactgttg gtcacagaga tgaaggactc gagatcctcc
     1261 agaaatgtcg cctcctgctc gtcggacccc tcgcgcgttc cgcgctcggc gttccatctc
     1321 tccgcggcga gagtggcaaa gctccggcgg gcatcggcgt cgagtacgac gggatacggg
     1381 ggaagaatcc ccactgacgg cacgggctcg accgattgct gtgtcaccgg gtcaaacagt
     1441 ttgattcggc tgatgacatt gccctcgagc acgatccgcg caggatattc tccaacctga
     1501 gagtacagat cgaagacgtt gcctcgcata ctgaactggc acatttcagt gactgatgag
     1561 accctttcgt agcccatttc gatgagacgc cgggcaagtg agtgtggaac aactcggtcg
     1621 ccggtgccaa gcgaggaaac cagcccggca aaatcctgag ctggtatcga cggatcgatc
     1681 aaagcctgga cggtcgtgaa gacgactgtc tcggcgtgag catagatctg gtcgagcgcc
     1741 cgggctctgc tgaatgacag cggacgtgag tactgggtat actccatgat ctcgacgccc
     1801 aactcaggca ggaccacgaa ttggcgcgtg tcgaccgtcc cgcaaaggcc tcgcaccatc
     1861 gaagagagtt cctctgcccg gtcgacgctc gggcacacca cgacaagagg ccggggacaa
     1921 cctgcgatca gcgctgcaag caggagctgg cgatagcccg tagagggcgt agacacccga
     1981 atcgagcctg gctcttccag cagctgcgcg aatctggcga acagttccgg agaaccggcc
     2041 tgcgcctgct gcgacagcag gtcgaaggaa ccggactgca tcagtccttt gccaccttgc
     2101 ggttgtacat gtcctgcgca gccatcagcc cattgcagat gatggttcga atgactgtcg
     2161 gcgtcttcgc gatggcgtcg atcacactcg actgttcaga tctggagaat ctcgacagta
     2221 catggtcgat gaccccatct gcgctgtccg gcttgccgat gccgagacgc acccgcacaa
     2281 acccctttcc gccacagttc tcggcaatcg accggacacc cttgtgacct gcggcggtag
     2341 cattgaaact gatgcggatt gctccaatcg gcaggtccat ctcatcatgg atcacgatga
     2401 ggttctcaag gagaatgttg cgttccttca tcattggggc gatcgcgacg ccggaggcgt
     2461 tcataaacgt ctgaggcttg accaggacta gccgaacagg ttccggaccg tcacacaggg
     2521 cgagatcggc ggctgcccag tgtgcgagtc gattgtgatt gaacctgcga accttgaatg
     2581 aatgtgcgat agcgtcgagg gccataaagc ccacattgtg ccgtgtttcg tcgtactcgg
     2641 aacctgggtt gcccagtcct gcgatcaata tcgttttcac agatggtata ctactcggga
     2701 ggagccggat ttcaagctgt gaacggttga caggggcccc gaacaccgta tactttcttc
     2761 gcattggtcg ggttcccaaa gtgccgaaag gccgcctcga ccgagcaatt cctcatggag
     2821 gtagtctatg tacgctgtca tcgaagcagc aggaaggcag tatcgggtcg agctgggcga
     2881 gaccgtcaag atggagaaga tcgccagcga acccggcacg ccgatcgtgt ttgacaaagt
     2941 cctcgtcgtc accactgacg ccggcactca aatcggcaag ccctacgttc tggacgcgaa
     3001 ggtgagcggt acgatccttc gtaccacgcg cgaccgcaag gtccgtgtct tcacctacaa
     3061 gaacaagacg acgcacaaga agctcatagg gcacagacag acatcgacct acgtgaagat
     3121 cacggagatc gggtaatagg ggtggtggac aatggcacac aagaaagcag gcggtaagtc
     3181 acgtaacggg cgggacagca acccacagta cctcggcgtc aagctgtatg atggtcaggt
     3241 tgtccagccg ggcaacatca tcgtgcgtca gcgcggcgag cacttcaagg ccggcagcgg
     3301 cgtcatgatg ggccgggatt ataccctctt cgccgtggtt gaagggacag tgaaattcga
     3361 gtcgctcaag accgtcaagc gcgtgcatgt gcttccagtc cctgagcagg cgaactagca
     3421 cgaacaagga gcaattccat tagagactca gctgaccgca ttctcgacga ggtcgtagtt
     3481 cgagtcactt cgggacacgg cggaaacgga gcaatgagct tccgccgtga gaaatacgtg
     3541 gagaagggcg gaccagacgg tggcgacgga ggcagaggcg gagacgtcat cttcgtggct
     3601 accgcccgct actcaagcct tcagttcttc gccaagaagc gcaccttccg ggccgaggca
     3661 ggagagggcg gcgcgggacg caacaagcac gggagcgatg ggcaagacat cacaatcctg
     3721 gttccgctgg gcaccgttct gacggacgtc gagtcagaag aagtcctcgc ggacctggtc
     3781 gacgagggcg agaccgttgt cgtggcccgc ggtggaagtg gaggccgtgg caacgcggcg
     3841 tttgccacat ctgtcgaccg agcccccagg tactccgaag aggggatacc ttcacaggag
     3901 cgtcggatca gacttcggct gaagatcctg gccgacgtcg gcatggtagg ctttccaaat
     3961 gccggcaaga gtactctgct gagcaagctt tccgcagcac atccgaaaat cgccgactat
     4021 ccgttcacga cgttgtcgcc gaacctcggc gtcgcgtttt cgccggaggg tgatcgcgtc
     4081 acgtttgcgg acatccctgg actcgtcgaa ggcgctgcgg atggacgtgg actcggcaac
     4141 aaattcctcg cccacatcga acgcagtcga gtcctcctgt ttctggtcga cggcagttca
     4201 gctgatcccg atccgatcca ggcgtatcac cttcttcgcc gcgagctgga acaatacagc
     4261 gagcagcttg ccggcaagcc tcagctggtc gtcgtcaaca agatcgacgc gatctcagca
     4321 cagaagcgta ccggcatccg tatgcgcttc gtccgtcaag gtatcgagcc tctcatgatc
     4381 tctgcactgg aaggcgtcgg tatcgacgcg atcatcctgc gcacgctggc acttgtgcag
     4441 tcggcaccta tccctccccg cgagcaggtg acgcgcatct acaaactgaa gccgtccgat
     4501 gaaaccttcg agatcacgcg gcttccagac ggcatgctct ccgcttctgg acgctatctc
     4561 gacaggatca tccatacgac cgacttcgag aagccgttcc aggttactct gctggaccag
     4621 aggatgcgca agatcggggt cgagaaagct ctccgcaaga agggggccca tgacgggcag
     4681 aacgtcatga tcgggggccg cgtgttcacc ctgggagaag agatagccaa gaagcgatga
     4741 agacggtgct ctacggggga gcgttcgatc ccgtccattg cggtcacgtc tttgtcggcc
     4801 atgaggcact tcgtctcatg gccccttgca tacttgtggt catccctacg gggctgccaa
     4861 atccggactt cggcaagcgt ctctcaggat cggacgcgga tcgcatggcg atgctgcgcc
     4921 ttgccttcgc aggagtgccc aacgtcgtca tctccgacat cgaactggac cgcggtccac
     4981 agccgtcgta cttcatagac acgatcgagc gtctccatcc gtcactgggt gacgagaagc
     5041 cggcacttct gctcggcgag gatcagctcc tctcgttcac cgagtggcac aggtaccgcg
     5101 acatcctgga ccacgttgaa ctgtggtttg tcccgcgggc aggcggacac cgtgcaggcg
     5161 gcccgcgggt agccgccacc gccctgttcg acatcaaccc gttcgatggc ctgtcgtcaa
     5221 gcctgattcg cgacaggctg cagcagggcc tccccgtcga tggcctggtg ccaccgtctg
     5281 tcgctgccta catagccgaa cacgcgctct acaagagcca gtgacatccc gggagagggc
     5341 ccgtatcgtc gctctcgtcg cgcagcgggt ctcgcagaag cgactcgagc acacgcttcg
     5401 cgtagaaaca ctggctgtgg cccttgcgcg tttctggggc gtctcggagg ataaggtgat
     5461 cctcgcatcc ttgcttcacg atgtcgcgag agatgaggca gagacatcgc tgctcgagct
     5521 tgcgagaggc agcagcgatc ccgttgttcg ggggatggca ggaacttctg ctccggtagt
     5581 cctccacgcc ccggtcgggg cactgatcgc acgacggaac ttcggcgtgc aagacccgca
     5641 gattctccag gcaattgccc tgcacacgac cggcggccct cacatggacc gccttgcgat
     5701 gatcgtcttt ctggccgact actgcgagtc aggtcgcagc ttcgcgggcg tagacgacgt
     5761 acgccttctg ttgttcgcca gcctcgaaga tgccatgctt cgcgcactcg aacagacgct
     5821 gacctacctg cgccaaaatg gtcgtccgat cgacccaaat acggctgaag ccgcgatggc
     5881 tttcgcgcac ccggatagag gagactcgca gcgcccttac cgcgcttgac agaagcctct
     5941 gcgccgtgta gaatacatga gtacctgatc gtgcgggagt agctcagggg tagagcatcg
     6001 gcttcccaag ccgagtgtcg cgggttcaaa tcccgtcttc cgctccagag gcggcatagc
     6061 caagtggtaa ggcaagggtc tgcaaaaccc tcataccccg gttcgaatcc gggtgctgcc
     6121 tccattgtta tatcctcaca tccctgccgg gatggcggaa ctggcagacg cacgggactt
     6181 aaaatcccgc ggtggtgaca ccgtgtgggt tcaactccca ctcccggcac caccttggtc
     6241 acggcgacgg cgctatgcct cggtatccga tcctgtgttc cccagactgc gcgtatgcct
     6301 gtagactctc cacgttatgt cagcattgcc catccgcgac agtggcacga atatctccgg
     6361 gttcgtgtcg gcgatctgag accccgatgc cagctggact cccatcttga tggtcgcaag
     6421 ataggtcttc tcgtacagca tggcgtcgac accgctcttc ttgcgcatcg tgccaaggac
     6481 gaagacacga gcgtacttga agtcccggtg cttgacccga gacttgagca gcttcaggac
     6541 tcccgtgtcg cgccgcaagt cggataggcc gtagacgtgg cgactgggcc tgtagggcgg
     6601 gtgggccccg tcgtattcct cgacaacttc attgagatca ggcagggtca ccgccatccc
     6661 gatgacaggg tggttcggct tcgtcatgtc ttccaccacg aagaccaggt cctcaatggc
     6721 gatcatcttc agcccagcag caagatagtc gaattcctct tcggagaact gttcgaactg
     6781 tgggtgaacc ctggtgtccc aggccacatt gtagaccgtc ttcacgcgcg cgatctcgtc
     6841 tgcccaatcg ctcatcttga cagatctgat ggcgacgccc tggcgtgcca ggcgctgctc
     6901 gaggtcctgc ccccgttttc gcatgtcatc gagtcgctcc atatagggag aagcagactc
     6961 atgtgtcatg cgaaatgcga accagtcgtt ctccttgacg ccccacttct cgagaaagcg
     7021 cgtgtagtac ggagggtgat acggttccat gatcatagga tgcttgtcga atccatcgac
     7081 aaggaggccg tcctcgccgt tggcgttgaa gcgagcaggc ccgatgatct tcaccgtacc
     7141 gctgtgctga aggtcttcgg tagccgcacc aagcaatgca gaggcaacct cctcgtcgtc
     7201 gatcgactca aatagtccga tccatccgac ctcgccatga accatgggtt catggtagga
     7261 ccgatcgacg aatgccgcga tgcgcccgac gggaaacccg tttctgcgcg caatgtagta
     7321 ctgcagaaag gagcaatgat ggaagaaggg attcgcaccc gggaatttcg tcttccacca
     7381 ggcgcgatcc gggttcagat cgctcatctc cgcagccagg acgggcatca cct
//
LOCUS       DATMZD010000131         3249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_63682,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000131 DATMZD010000000
VERSION     DATMZD010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63682"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1265)
                     /locus_tag="VN478_02755"
     CDS             complement(<1..1265)
                     /locus_tag="VN478_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21629.1"
                     /translation="MAEAAPIGFVLGTKPASSLEFYVLVDQHNYLEMDDVVFVRSAVE
                     GYAGVEAVTFYGTVIEVQQYHEGIQFDSDTTIVRQGIMPAGVAYVGKVQVTKLEPEVF
                     VAPHPADFVYRATGADLDKALSFDVMKQRVPVGIMRNKEPFYVNFEFLNGAKGAHVSI
                     SGVSGVATKTSFALFLLYSMLHFEGTRQGDVQMQDGMPFADKAQARSIVFSVKGEDLM
                     FLDKPNKGLTDDHRAVYAKMGLPTTPYTDVRFFTSPKADSEIPSPSTEQRQEGVTPYV
                     WTMRQFAQDRLLPFLFAEGDQETTNLYGLIQHLTNRLDYLASDHNMENDKHSEALVYQ
                     GQTIGDLQDLITILETDIMGPAESEDEGKKSKRPPSGGWFTSFDNQGTQLAFLRRLRV
                     AADAMKGVIRSDVDHPEKYRFEFNQHRVTV"
     gene            complement(1443..2300)
                     /locus_tag="VN478_02760"
     CDS             complement(1443..2300)
                     /locus_tag="VN478_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21630.1"
                     /translation="MNDKRFVRMAGLCLIVALMVGTAAVIGVFFRGEGTPTTIVSPRG
                     EEYAMISAGVYRYNSQRMVSEGAGWDIFTLFFAVPAMLAVLFGLSRGVLRARLFALGL
                     LVYFFYQYLCYALGWAFGPLFPLFIVIFAASLAGALWIASTVDVAGLPAQLSKRFPRR
                     GMAILCAFMAVVLLAMWGQRIAAGLHGDLATAMLLGQTTMVVQVLDLGLLVPLSLFTA
                     LAAWRGWPVGYLLSSVFVVKAVAMAAAICAMLLSAWATEGTLEVAPLLIFGTAAAAAS
                     WLGVRMYRR"
     gene            complement(2580..2945)
                     /locus_tag="VN478_02765"
     CDS             complement(2580..2945)
                     /locus_tag="VN478_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWQ21631.1"
                     /translation="MARPIHFELSAVDPARAITFYKAVFGWTIEPAGGPVEYYLITTG
                     PTTEPGINGAITRTRDGTVGTVNTISVASVEESVVAVTANGGTVLMTPQPMPGVGRLA
                     MCKDTEGNVFGILQMEARP"
     gene            3065..>3249
                     /locus_tag="VN478_02770"
     CDS             3065..>3249
                     /locus_tag="VN478_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21632.1"
                     /translation="MKNLTRLSDLRRRFTIALLLAYVGVAIGLAVVVPHQLHSAETRW
                     RSTLSSTAADRAGILDT"
BASE COUNT          621 a          985 c         1055 g          588 t
ORIGIN      
        1 acggtgacgc ggtgctggtt gaactcgaag cggtactttt cggggtggtc cacgtcgctg
       61 cggatcacgc ccttcatggc gtcggccgcc acgcgcaggc ggcgcaggaa ggcgagctgg
      121 gtgccctggt tgtcgaagga ggtgaaccag ccgccggacg gcgggcgctt gctcttcttg
      181 ccctcgtcct cggactcggc cggccccatg atgtcggtct ccaggatcgt gatgaggtcc
      241 tgcaggtcac cgatagtctg gccctggtac acgagggctt cgctgtgttt gtcgttctcc
      301 atgttgtggt cggaggccag gtagtccagc cggttggtga ggtgctggat caggccatag
      361 aggttggtcg tctcctggtc gccctcggcg aagaggaagg ggagcaggcg gtcctgcgca
      421 aactgccgca tggtccagac gtagggggtg acgccctcct ggcgctgctc ggtgctgggc
      481 gacgggatct cgctgtctgc cttgggggac gtgaagaagc gcacgtcggt gtagggcgtg
      541 gtgggcaggc ccatcttggc gtagacggcg cggtggtcgt cggtcaggcc cttgttgggc
      601 ttgtcgagga acatcaagtc ctcacctttc acgctgaaaa cgatgctgcg tgcctgggcc
      661 ttgtcggcaa agggcatgcc gtcctgcatc tgcacgtcac cctgcctcgt cccctcgaag
      721 tggagcatgg agtacaggag gaagagggcg aaggacgtct tggtagccac gccgctgacg
      781 ccgctgatgc tgacgtgcgc acccttggcg ccattgagaa actcgaagtt gacgtagaag
      841 ggctccttgt tgcgcatgat ccccacgggc acgcgctgct tcatgacatc gaagctgagt
      901 gccttgtcca ggtcggcgcc ggtggcgcgg tagacgaagt cggcggggtg cggggcgaca
      961 aacacctcgg gctccagctt ggtgacctgc accttgccca cgtaggcgac gcctgcaggc
     1021 atgatgccct ggcgcacgat ggtcgtgtcg ctatcgaact ggatgccctc atggtactgc
     1081 tggacctcga tgacggtgcc gtagaaggtg acggcctcga cgccggcata cccctcgacc
     1141 gccgaccgca cgaagacgac gtcgtccatc tccaggtagt tgtgctggtc gacgaggaca
     1201 tagaactcca gcgacgatgc gggcttggtg cccaggacga acccgattgg tgctgcttca
     1261 gccatgccga ccctcccgct catttgtcaa tgcgttcagt atagtccaaa acgagagggc
     1321 tgatagcaat cacagaaagg gcggcgcgcc acaagacatg gaatgggttc ccgcctgcgc
     1381 gggggtctac ctgtcgagca tgcttgggat cgccgtcagg acggactgcc tcaggacaag
     1441 gactacctgc ggtacatccg gacgccaagc caggatgccg cggcggcggc cgtgccgaag
     1501 atgagcaagg gggcaacttc cagcgttccc tccgtggccc atgccgacag cagcatcgcg
     1561 cagatggccg cagccatggc gaccgccttg acgacaaaca ctgagctgag caggtatccg
     1621 acgggccagc cgcgccatgc cgcgagggct gtgaacagtg agagcgggac gagaagcccc
     1681 aggtccagga cctggacgac catcgttgtc tgaccaagca gcatcgcagt cgcgagatcg
     1741 ccatggaggc cggccgcgat ccgttgaccc cacatcgcca gcaggaccac cgccatgaac
     1801 gcgcacagga tggccattcc tcgccgaggg aagcgtttcg acaactgggc cggcaggccg
     1861 gcgacatcga cggtacttgc gatccacagt gcacccgcca ggctggcagc gaagatgacg
     1921 atgaacaggg ggaacagcgg tccgaaggcc catcccagcg cgtagcacaa gtactggtag
     1981 aagaagtaga cgagaagccc gagggcgaac aaccgggcgc gcaacacacc acgcgacagg
     2041 ccaaacagga cagcaagcat cgccggcaca gcgaagaaca gcgtgaagat gtcccatccg
     2101 gcgccttcgc tgaccatgcg ctgtgagttg tatcggtaga ctcccgcgga gatcatcgcg
     2161 tactcctcgc cacggggact gacgatcgtg gttggcgtcc cctcaccgcg gaagaacact
     2221 ccgatcactg cagccgtgcc aaccataagg gcgactatga ggcatagtcc tgccatccgt
     2281 acaaagcgct tgtcgttcac gagagccccc tcctgaggtg cgtttggagt tgcggccatg
     2341 ggccgtcaac caaccaagta tatgacatgg agccccgagc cgcaatgtgc cgacgagtga
     2401 ggacctccgc cttggggagc tacttgtcga gcatgcccag gatcatggtc aggatggaca
     2461 gccccaggac cgtggcggcc aggacggcta cgtcgacaac caccgggata acgtgccaga
     2521 gatcttgagg atccatgaat gcctccccgg ttgtacaaac gtactccgcg tgatgcggtt
     2581 cagggtctag cctccatctg caggatgccg aagacgttcc cctccgtgtc cttgcacatg
     2641 gccagcctgc caacaccggg catgggctga ggcgtcatga ggacggtgcc accattggcc
     2701 gtcaccgcaa cgacgctctc ctctaccgag gcgacgctga tggtgttcac ggtgcctacg
     2761 gtgccgtcgc gcgtgcgggt gatcgccccg ttgatgccgg gttccgtggt ggggccggtg
     2821 gtgatgagat agtactccac gggtccgcct gccggttcaa tcgtccagcc gaataccgcc
     2881 ttgtagaagg tgatcgcgcg ggcggggtcg accgcactca actcaaagtg aatcggacga
     2941 gccatagtga gcctcctgac caatgtgtga ccgctgcggt ctctgtgcac agtgtatgcg
     3001 gcgccactgc catgtccatg ccggttgatt gggagcgttt gcagtacata atatcattcg
     3061 gaacatgaaa aacctgacca ggctgagcga cctccgacgt cgttttacaa ttgctctgct
     3121 cctcgcgtac gttggtgttg ccataggctt ggctgtcgtg gtgccacatc agctccattc
     3181 tgctgagacc cgatggcgca gcacactgtc atcgactgcc gccgaccggg ccggcatcct
     3241 ggacaccat
//
LOCUS       DATMZD010000132         4022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_94240,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000132 DATMZD010000000
VERSION     DATMZD010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94240"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            222..1172
                     /locus_tag="VN478_02775"
     CDS             222..1172
                     /locus_tag="VN478_02775"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HWQ21633.1"
                     /translation="MAKKASVENKSKRTGYSVVIVPQYEGRARQFRVSRLAIYTAITA
                     FLLFVSGFVYVIGQNTHLRNTVAQYDGMGLDQVVRLQATQLEAANEAITTNNAELAAL
                     KKYVSYLGTLDAKVRKTLGVGANTVSLASVLKSTATPTLSVSRGLAAGATQLSTSAAV
                     VQKDAEAREKNLIVLQDAASKYNSMLAQTPSLWPLYGLITSDYGWRSNPFGGSGGEYH
                     DGVDIAAPYGTAVRATADGKVEQAGWNSSYGISVTLYHRDGIETLYGHMCRAAVSAGQ
                     TVKKGQVIGYEGSTGRATGAHVHYEVMVHGSSVNPMTYLD"
     gene            1190..1570
                     /locus_tag="VN478_02780"
     CDS             1190..1570
                     /locus_tag="VN478_02780"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HWQ21634.1"
                     /translation="MAFGPSKTSTDSKTASVFANDLIVKGDVDNKGDLRIEGQLHGSV
                     TGHGMITIAEQGEVHGNVEGRAVIVMGRVEGNVRGTEKVEVLEKATVKGDVTSARISV
                     EEGASVSGKFDTTPREVEVPGTKS"
     gene            1914..2879
                     /locus_tag="VN478_02785"
     CDS             1914..2879
                     /locus_tag="VN478_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21635.1"
                     /translation="MGEGKLAEFPARTILEVLFTKDPSALLSVTTPAARYDLVVGRSG
                     IRYATRGQLSGDTAVFLLLMETEGTFNLGPVTSDRVSNCVFETLADVDSRYANWRKRL
                     TGTDLRFLDPGRLYWWRSKLSKDVQQLPAPEFEVAQLTRDRAQTIDSLASVLGQDVIE
                     VSRVLTRMTHLESFEIVPFIRPPRQVAYRCVVASRDALLANAFWQNVCGGEMPLQWDS
                     HELLYRRTISHASYELATVFVMQVLDDTMKDMVRLADILIFLLSDLPSDEERYLADLR
                     TLNPGMSVFFWGSRRRKWARHIQQSVHFTTPLERDAVLQSLDAFL"
     gene            3202..3585
                     /locus_tag="VN478_02790"
     CDS             3202..3585
                     /locus_tag="VN478_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21636.1"
                     /translation="MRQQKREHTLNMQKLVTLAACLVIGYLIWSTIAALSYLGKADRE
                     ATRTFAELQRVSAQTLQQQKAMEYHATSAYVEKVAREDMLMSRKGDTVILFPVDRTAP
                     APVPTEPAGIPRVLNQIISWVKGWF"
     gene            3665..>4022
                     /locus_tag="VN478_02795"
     CDS             3665..>4022
                     /locus_tag="VN478_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018978489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HWQ21637.1"
                     /translation="MTTLMAEEHRIKLTEGIVVKIVPFGAFVKTDTNEVGLVHISEIS
                     DTYVKDVNQFLKEGDKVTVRVIGRNKDGKLNLSIKAAKAAGNADGTPVVAAAEGSSPS
                     QASAPSAPSPRPSYSSR"
BASE COUNT          830 a         1126 c         1255 g          811 t
ORIGIN      
        1 gtcgcttacc aatgcccagg gcagcaaggt gtacgcgaag cggatcgaga ccgggaagag
       61 cgacactgcc ctgagtcacg aggagcagga gctgctcgat agacatgggc ggccttgacg
      121 tggggttgta ttttgttccg cgttcctata ctgtgtacat gtctgtcgtt ggtgggtaag
      181 tattgcccat cctgacggaa tccctgacga gtgaggcgat gatggcgaag aaggcatcag
      241 tcgagaacaa gagcaagcgc actggctatt cggtcgttat cgtcccacaa tacgaagggc
      301 gcgctcgtca gtttagggtt tcccgtcttg ccatctacac ggccatcaca gcgtttctcc
      361 tctttgtctc tggcttcgtg tatgtcatcg gacagaacac tcatctcagg aacacagtgg
      421 cacagtacga cggcatgggc ctggatcagg ttgtccggtt gcaggcaact caactcgagg
      481 ctgccaatga agccattacc acgaacaacg ccgaactagc cgcactcaag aagtacgtct
      541 cgtaccttgg gacgcttgac gccaaggtcc gcaagacgct gggcgtgggc gcgaataccg
      601 tctctctggc gtccgtcctg aagtcgacag cgacgccgac tctctccgtc agccgtggct
      661 tggcagcggg cgcgacacag ctttccacgt ctgctgccgt cgtccagaag gacgctgagg
      721 ctcgtgagaa gaatctgatc gtccttcagg acgctgcttc caagtacaac agcatgctgg
      781 cgcagactcc ttcgctctgg cccctctacg ggctcatcac gtcggactat ggctggcggt
      841 cgaacccgtt tggcggcagc ggaggcgaat atcatgacgg tgtagacatc gcggctccat
      901 atggtactgc agtcagggcc acagccgacg gcaaggtgga acaggctggc tggaacagca
      961 gctacggcat ctcagtgacc ctgtatcacc gcgacggcat cgaaacgttg tatggccaca
     1021 tgtgccgcgc ggccgtatcg gcagggcaga ctgtcaagaa gggtcaggtc atcggctatg
     1081 aggggtcgac ggggcgtgcg accggggccc acgtgcatta cgaggtcatg gtccatggca
     1141 gttcagtcaa cccgatgaca tatctcgact aggtgtggag gagacagcca tggcatttgg
     1201 gccatccaag acttcaacgg acagcaagac agcatctgtc ttcgccaatg acctcatcgt
     1261 gaagggtgat gtcgacaaca agggcgacct gcgcatcgag gggcagctgc atggcagcgt
     1321 gaccggccac gggatgatca cgatcgccga acagggtgag gttcacggca atgtcgaagg
     1381 gcgtgccgtc atcgtcatgg ggcgtgtcga gggaaacgtc aggggaaccg agaaggtcga
     1441 ggtcctcgag aaggctactg tcaagggcga cgtaaccagc gcgcgcatct cagtcgagga
     1501 aggtgcatca gtctcgggga agtttgatac gacaccccga gaggttgagg tcccgggcac
     1561 gaaaagctag gcttcctgca catatgcacg gtacggccgg cgtcatcaac agggcgccgg
     1621 tttgtcatat gtcggccgca tgctcggatg ccgtccaccc tagactgcac gtgcggccgt
     1681 gagtcgcagc ggaccgacgt tcatctgtgg cgttcccgag ggcgagcaaa caagtgccac
     1741 gatacgcgca tcggtcgcgc gttctggcgc atgacacggc gcctggcaat gtccgtcggg
     1801 ggaggcgccc ggcgttcgct attgcgccag ctcttccggt gctatacttt gaaccacgat
     1861 atcgactgat ctgggctacg gtgacgtgaa ggactgtgtg ctggagggat acgatgggcg
     1921 aaggcaaact ggccgagttt ccagctcgta cgattctcga agtgctgttc accaaggacc
     1981 cgagcgcact tctatctgtc acgacgcctg cggcacggta cgatcttgtg gtcggtcgga
     2041 gtggcatccg ctacgcgacc cggggacagc tgagtggcga cactgccgtg ttcctgctgc
     2101 tcatggagac tgaaggcacg ttcaacctcg ggcctgtcac gtcggatcgt gtgtcgaact
     2161 gcgtctttga gaccctggcc gatgtcgact ccaggtatgc gaactggagg aaacgcttaa
     2221 ccgggaccga tcttcggttc cttgaccccg gacgcctcta ctggtggcgg agcaagctca
     2281 gcaaggacgt acagcagctt cccgcgccag aatttgaggt cgcgcagctg accagagacc
     2341 gggcgcagac gatcgattcg ctggcgtctg tcctcggtca ggatgtgatc gaggtctcac
     2401 gagtgctgac gcgcatgacc cacctggagt cgttcgaaat cgtgccattc atccgtcccc
     2461 ctcgtcaggt tgcctatcga tgtgtcgtgg cttcacgtga cgcattgctg gccaatgcct
     2521 tctggcagaa cgtctgcggt ggggagatgc ctctccagtg ggacagtcac gagctgctct
     2581 accgcaggac catctcacat gcttcctacg agttggcgac cgtgtttgtg atgcaggtgc
     2641 tggatgatac gatgaaggac atggtgcggc ttgccgatat cctcatcttt ctcctctctg
     2701 acttgccgtc cgatgaggag cgctacctgg cggacctgag aaccctcaac ccaggcatgt
     2761 ccgtgttctt ctggggttcg agaaggcgga agtgggctcg ccacatccag cagtcagtac
     2821 acttcacgac gccgctggaa cgcgatgccg tcctgcagtc tcttgacgcg tttctgtagg
     2881 tgaagtgact ggaggacgga atgacacggg ttggctgcat acggtcgagc tgatggaggc
     2941 tgatgagcgg atgtgcacat gcaaggagac tcatgtcatg cgcgtgggca tctccagagc
     3001 tggcaacaca ctgcgtgagc ggtgaaccga agcggccaca ttcgcgttgc acgtcacgct
     3061 gcttgacgtg gccacattgg atgagtacta tgacctcagg agccggggcc gttcgggtgt
     3121 tcgggcggcc gagacagcga gtagactgcg catgaaccag ctcggcgctg tggatccggc
     3181 ttgtgagggg tgagaccttt gttgagacaa cagaagcgcg agcatacgtt gaacatgcag
     3241 aagctggtga cgcttgctgc atgtctggtc atcgggtatc tcatctggag cacgatcgcg
     3301 gcactctcct atctgggcaa ggcggatcgt gaggcgacgc gcacatttgc cgaactgcag
     3361 cgggtcagcg cacagacgct gcagcaacag aaggcgatgg aataccatgc gaccagtgcc
     3421 tatgtcgaga aggtcgcgcg tgaggacatg ctcatgagca gaaagggcga cactgtcatc
     3481 ctgttccccg tggatcgcac agcgccagcg ccggtcccca ctgagccggc aggcattcct
     3541 cgcgtattga atcagatcat ctcatgggtc aagggatggt tctagggttg cattttggct
     3601 tggacactgt atgctgtgtt attggagcct gccgcgtcga cagagctatc cccagcagag
     3661 gtgagtgact actttaatgg ccgaagagca tcgcatcaag ctaaccgaag gcattgttgt
     3721 caagatcgtt ccgttcggag cgttcgtcaa gacagatacg aatgaggtcg gcctggttca
     3781 catctcggag atcagcgata cgtacgtcaa ggacgtcaac cagttcctca aggaaggcga
     3841 caaggtcact gtccgcgtca ttggccgcaa caaggacggg aaactgaacc tctctatcaa
     3901 ggctgccaag gctgccggca acgccgacgg aacacctgtc gtcgcagctg cggaaggcag
     3961 ttcacccagc caggcctctg caccaagcgc gccgtcaccc cgtccctcct acagttcgcg
     4021 tc
//
LOCUS       DATMZD010000133         2546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_84168,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000133 DATMZD010000000
VERSION     DATMZD010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84168"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="VN478_02800"
     CDS             complement(<1..560)
                     /locus_tag="VN478_02800"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HWQ21638.1"
                     /translation="MKPQIGVIGLGKFGRSCAIRLAQLGFEVLGIDDKQENVDAVKDE
                     IALAVIADTTDPAELEAAGISNCDTVVLTIGAEIEHSVLTAVVLKEMGIRHLVARASS
                     DLHGKLLMRIGADDVVFPEHDMAIRLANRLSLSSIYDFLESSPDYDILEVPIHRDAAG
                     KTLRDLDYRAKYEINVVGIRRGGEVLV"
     gene            complement(572..1960)
                     /locus_tag="VN478_02805"
     CDS             complement(572..1960)
                     /locus_tag="VN478_02805"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HWQ21639.1"
                     /translation="MPAAKHSKPGRLRRLMPASPAQRIFLSFTVVILVGTFLLLLPQS
                     NTGVRPGFLRALFTATSATCVTGLVVFDTGSFWSTSGQVVILLLIQIGGLGYMVLTTT
                     LMAAFRNARLHVRQTGDFRESVSAPSHRDPRSFARLIASTVIICEGLGSVFLSLRFLR
                     DMPAGKAIWNGVFTSVSAFCNAGFDILGRGMTSLETYAGDLTVNLVVPVLIILGGLGF
                     LVIDELRQRLTLTRHKRLSTHTRTVLSISTVLVVGGALMIFILENMSPGAFASLSLKD
                     RVLVSWFQSVTARTAGFDTVRLGTMHASSLMLIIVLMFIGASPGGTGGGVKTTTFAVV
                     AAFIWSSVIGSDQAHLGSRGVRTDSVKKAVVVFSLSLVLVFGGTFLLAMMDGHKFSTL
                     SLLFEVTSAFGTVGLSTGITPDLSAGSKVVLIATMLAGRVGVLTAMLTLLAPVRKLER
                     RLHFAEDEIAIG"
     gene            2171..>2546
                     /gene="mscL"
                     /locus_tag="VN478_02810"
     CDS             2171..>2546
                     /gene="mscL"
                     /locus_tag="VN478_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008337977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HWQ21640.1"
                     /translation="MWNEFKKFALKGSMIDLAIGVIIGGAIGKVITSLVNDILMPPIG
                     LLLGRVNFASLYINLSRTKYPTFDAAKAAGAPTINIGLFLGTIIDFLIISLVIFLIVR
                     EMNRLRERSERNIPAPAPTTKVC"
BASE COUNT          530 a          746 c          786 g          484 t
ORIGIN      
        1 accaggacct cacctccgcg cctgatgccg acgacgttga tttcatactt ggcacggtag
       61 tcgaggtcac ggagcgtctt gccggcggca tcgcggtgga tcggaacctc gagaatatcg
      121 tagtccgggg aagactcgag gaagtcgtaa atgcttgaaa ggctcagccg gttggccagg
      181 cggatggcca tatcatgctc agggaagacc acgtcgtctg cgccgatgcg catcaagagc
      241 tttccatgca ggtcggacga ggctcgtgcc accaggtggc gtatgcccat ctccttgagg
      301 acgacggcag tgaggacact gtgctcgatc tctgctccga tggtcaggac gacagtgtcg
      361 cagttgctga tgcctgcggc ctccagctca gccgggtcgg tcgtgtctgc gatgaccgcc
      421 agggcaatct cgtccttcac tgcgtcgacg ttctcctgct tgtcgtcgat gcccaggacc
      481 tcaaatccga gttgagcaag gcggatggcg caagaccttc caaacttccc aagaccaatg
      541 acgcctatct gtggtttcat ggtagtgcct cctatcctat cgcgatttcg tcttcggcga
      601 agtgaagccg gcgttcgagc ttgcgaacgg gagcaagcag tgtcaacatg gccgtgagta
      661 ccccgacgcg tcctgccagc atggttgcga tgagtacgac tttggatccg gccgacaggt
      721 cgggcgtgat gccagtactg aggcccacgg tcccgaacgc cgacgtgacc tcgaatagga
      781 ggctgagcgt ggagaacttg tgcccgtcca tcatggccag caggaacgtc ccgccaaaga
      841 ccaggaccag gctgagagag aacacgacga cagccttctt gacgctgtcg gtgcggacgc
      901 cgcggctgcc gaggtgtgcc tggtccgatc cgatgaccga actccagatg aatgcggcga
      961 cgaccgcgaa cgttgtcgtc ttcacgcccc cgcctgtgcc gccgggagaa gcaccgatga
     1021 acatcagcac gataatgagc atcagtgaac tggcatgcat ggtgccaagg cgcacagtgt
     1081 caaatcctgc tgtccgcgca gtcaccgact ggaaccagga aaccagtacg cggtccttca
     1141 gggagagtga ggcgaacgcc cctggactca tgttttcgag gatgaagatc atcagagctc
     1201 cgccgacgac aaggaccgtc gagatgctca ggaccgtccg ggtgtgcgtc gacaacctct
     1261 tgtgtcgcgt gagagtcaag cgctgcctca actcgtcgat gacgaggaag ccaagcccac
     1321 ccaggatgat gagcacgggc actacgaggt tgaccgtgag gtcgcctgca taggtctcaa
     1381 ggctcgtcat ccctctgcct aggatgtcga agccggcgtt gcagaaggcg ctgacgctcg
     1441 tgaacacgcc gttccagatg gccttgcccg ccggcatgtc tcggaggaag cggagcgaca
     1501 ggaacacgga ccccagtccc tcgcagatga tcaccgtgga agcgatgaga cgggcgaagc
     1561 tgcgtggatc gcggtgcgat ggcgcactga cgctctcgcg gaagtcgccg gtctggcgca
     1621 cgtgcagtct cgcattccgg aaggccgcca tgagggtcgt cgtaaggacc atgtagccga
     1681 ggccgccgat ctggatgagc aggaggatca cgacttgccc ggacgtcgac cagaacgagc
     1741 cggtgtcgaa gacgaccaga cccgtcacgc aggtggcgct tgtagccgtg aacagggctc
     1801 tgagaaaccc gggtcgaaca cccgtattcg actgcggcag cagcaacagg aaggttccga
     1861 caaggatgac gaccgtgaaa ctcaggaaga tgcgttgagc gggactcgcc ggcatgagcc
     1921 gacggagacg accgggtttc gagtgcttcg cggcgggcat cactgtcggg tcctccatct
     1981 actgttcaca agcagaagta tatctccgcg cagaccgcat gcaacgtttt ccgtcgttcc
     2041 ccccgagtat gtcggttcaa tacgaggggc gcctttcccg agcctttctg ttcaaggcga
     2101 tgagtgctct gccgcccggg tgggctcgcc tggaagagcg tgcctgctat acttgaccag
     2161 gaggtggtct atgtggaatg agttcaagaa gtttgccttg aagggcagca tgatcgacct
     2221 ggcgattggc gtcattatcg gcggcgccat cgggaaggtc atcacgtctc ttgtcaacga
     2281 tatcctgatg cccccgatcg gtctgctgct ggggcgcgtg aatttcgcca gcctctacat
     2341 caacctgagt cgcacgaagt atccaacctt cgacgccgcg aaagctgccg gagcgccgac
     2401 cattaacatc ggactgttcc tcgggaccat catcgatttc ctgatcatct ccttggtcat
     2461 cttcctcatc gtaagggaga tgaacaggtt gagagagagg tccgagagga acatcccagc
     2521 ccctgcaccc acaactaaag tgtgcc
//
LOCUS       DATMZD010000134         3142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_14119,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000134 DATMZD010000000
VERSION     DATMZD010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14119"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VN478_02815"
     CDS             complement(<1..188)
                     /locus_tag="VN478_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HWQ21641.1"
                     /translation="MQNAFGYDGLAGPGSPESKETLLASIAEEVVSCQKCKLGATRTK
                     AVPGVGSAHAHICFVGEGP"
     gene            complement(190..732)
                     /gene="dut"
                     /locus_tag="VN478_02820"
     CDS             complement(190..732)
                     /gene="dut"
                     /locus_tag="VN478_02820"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HWQ21642.1"
                     /translation="MDPKSAERRVGVRRLAPGAGQLARATPGSSGYDLSSAEDRELTV
                     QPGGVVLVRTGLALEMPPGLEGQVRSRSGLAARSAVFVLNSPGTIDSDYRGEVKVVLA
                     NLGDAPFSILPGDRIAQLVFAEIPDVCLVSVDEISQTERGSGGFGSTGGKPLTSMPRL
                     RKARRVCPDGEAPSGSGSSD"
     gene            complement(713..1966)
                     /locus_tag="VN478_02825"
     CDS             complement(713..1966)
                     /locus_tag="VN478_02825"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HWQ21643.1"
                     /translation="MVISNGERKPEVITLSNGIRVVFDVDTGHPTAAVGALVASGSRF
                     EDDHTRGLSHFMEHILFKGTEHRSSLEISSAIENVGGELNAFTDTQYTMFYMRVPSIH
                     TPLALDVLSDILLHPLFDPAAIELERRVIEQEIYSFEDSPDDVVTDELLKGVYGSDPV
                     ASNPLGTVESVRAFVRSDFVEYFHRHFAAPDIVLSLAGDVDIDACARFLEDSFGQVAK
                     GPARAEWGIPTRAAVHKETERPTEQVYMAMALPGFAQYSKQGSVLNLVSSIFGGNMSS
                     RLFQRAREKEALVYSIGSFPVAFSNTGLLGVSAESSPQNASRVQQVICEEIESMRQDK
                     ISMAELQHAKDTVLGGFLLSLESFFRRMHRNASELYMRDRIRTVADVTEQVNAITLGE
                     ALDVIDEVFDTAQLAVSIVHGPEKR"
     gene            complement(1953..>3142)
                     /locus_tag="VN478_02830"
     CDS             complement(1953..>3142)
                     /locus_tag="VN478_02830"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014452852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HWQ21644.1"
                     /translation="RAPEEIRPIGVDVGVLPMSHGSGLFSRGQTQVLSIVTLGGKKEG
                     QLVESLEEEVTKHYMHYYNFPPFSVGETRPMRGPGRREIGHGALGERALLPVIPDETE
                     FPYSIRVVSEVLESNGSTSQASICGSTLALMDAGVPITAPVAGIAMGLMKEGDKSVIL
                     TDIQGAEDANGDMDFKVAGTAKGITALQMDIKIHGVDSELLSAALDHARTARLFILDK
                     MLSVLPAPRGEVNPLAPRIFTMHIPGDKIGALIGPGGKVIKKIIADTGCDIDIEDDGS
                     VFVTAPDGAKGQIAMDAINAITEDIVVGKVYMGKVTELRDFGAFVEIAPGKSGLLHVS
                     QVSDSYVKDIHSVLKVGEQILVKTLPLKDDGKISLTRKGLTGGTADKAAPESHDDHRH
                     GDQ"
BASE COUNT          666 a          961 c          883 g          632 t
ORIGIN      
        1 ggcccttcac ctacgaaaca gatgtgcgcg tgagctgatc caacgcccgg caccgccttg
       61 gtgcgagtgg ccccaagctt gcacttctga catgagacaa cttcctcggc aatcgatgcc
      121 agcagagtct ctttcgactc gggactacca gggccagcga gaccatcgta tccgaacgcg
      181 ttctgcatct cagtcagagg atccagaacc tgagggtgcc tctccatccg ggcagaccct
      241 gcgcgctttc ctcaagcgag gcatgcttgt gagaggcttg ccgccagtcg atccaaagcc
      301 gccactgcct ctctctgtct gcgagatctc gtcaaccgac acgagacata catccggaat
      361 ctctgcgaac acaagctgcg cgatgcggtc cccaggaaga atcgagaatg gagcgtcacc
      421 aagattcgcc agaaccactt tcacttcacc acggtagtcg ctgtctatag tgcccggcga
      481 gttgaggacg aatacggcgc ttctcgcggc gagacctgaa cgactgcgaa cctgaccctc
      541 aaggccggga ggcatctcga gcgcaagccc agtccgcaca agcacgacgc caccaggctg
      601 tactgtcagc tcgcgatcct ccgcgctgga caggtcatag cccgacgatc ccggtgtcgc
      661 gcgtgccagc tgaccagcgc cgggggccag tctacggaca cctacacgcc gctcagcgct
      721 tttcgggtcc atggacgatc gacactgcca actgcgcggt gtcaaagacc tcgtcaatga
      781 cgtccagagc ttcgccgagc gtgatcgcgt tgacctgttc cgtgacgtcc gcgacggtcc
      841 gtatgcggtc tctcatgtag agctctgaag cgtttcgatg catgcggcgg aagaaggact
      901 ccaacgacag caggaatccg ccgagaacgg tgtctttggc gtgctggagt tcggccatgc
      961 tgatcttgtc ctgccgcatg ctctcgatct cctcgcatat gacttgctgg acacgcgacg
     1021 cgttctgagg agagctctca gccgagacgc ccagtagtcc ggtgttgctg aaggccacag
     1081 gaaatgagcc aatgctgtag acgagagctt ccttctcacg tgccctctgg aacaggcgac
     1141 tcgacatgtt gccaccgaaa atgctggaga cgagattcag gacggatccc tgcttcgagt
     1201 actgagcaaa gccgggaaga gccatcgcca tatagacctg ttcggtggga cgctcagtct
     1261 ctttgtgtac ggcggcccgg gtcgggattc cccattctgc gcgggccggt cccttggcaa
     1321 cctgcccaaa cgaatcctcc aggaaacgag cacacgcatc gatgtcgacg tctccagcga
     1381 gagagagaac gatgtcaggc gcggcaaagt gcctgtggaa gtactcgacg aagtcactcc
     1441 gcacgaacgc acgtactgac tccactgttc ccagcggatt cgaggcgacg gggtcgcttc
     1501 catacacacc cttgaggagc tcatcagtga cgacgtcgtc cggactgtcc tcgaacgaat
     1561 agatctcctg ttcgatgacc ctgcgctcga gttctatggc ggcaggatca aagagcggat
     1621 gcagcagaat atcggagagc acatcaagcg caagaggtgt gtgaatcgac gggacacgca
     1681 tgtagaacat cgtgtactgg gtgtcggtga atgcattgag ctcgccgcca acgttctcga
     1741 tggctgacga gatctcaagc gagctccgat gctctgtacc cttgaacagg atgtgctcca
     1801 tgaagtggct caaaccacgc gtatgatcgt cctcaaaccg ggagccacta gcaacaaggg
     1861 ccccgaccgc tgcggtcggg tgccctgtgt cgacgtcaaa aacgacgcgg atgccgttgc
     1921 tgagtgttat gacttccggt ttgcgctctc cgttactgat caccatggcg gtggtcatcg
     1981 tgagactcgg gagcggcctt gtcagcagtc ccaccagtca gtcccttgcg cgtaagagag
     2041 atcttgccat catccttcag aggcaaggtc ttgacgagga tttgctcacc aaccttgagc
     2101 acggaatgaa tatccttcac atacgaatca gatacctgag aaacgtggag gaggccggac
     2161 ttgcctggtg caatttccac gaaagcgccg aagtcgcgca actcggtgac cttgcccatg
     2221 tagacctttc ccaccacgat gtcctcggtg atcgcgttga tggcatccat ggcaatctgg
     2281 cccttggctc catccggtgc agtcacaaat actgacccgt cgtcctcaat gtcgatatcg
     2341 catccagtgt cggcgatgat cttcttgatg accttgccgc caggaccgat gagagcgcca
     2401 atcttgtctc cgggaatatg catcgtgaag atgcgtggag ccagtgggtt gacctcgccg
     2461 cgaggagcag gaagaaccga cagcatcttg tccagaatga agaggcgcgc cgtccgtgca
     2521 tgatccagag ccgccgacag cagttcgctg tccacgccat gaatcttgat gtccatctgc
     2581 agtgccgtga tgcccttggc cgtaccggcg actttaaagt ccatgtcgcc attggcgtcc
     2641 tctgctccct ggatgtcggt caggataacg ctcttgtcgc cttccttcat caggcccatc
     2701 gcgatgccgg caaccggcgc cgtgatcggc actccagcat ccatgagcgc cagtgtgctg
     2761 ccgcagatgg acgcttgtga cgtcgaacca ttcgattcaa gcacttcgga aacaacgcgg
     2821 atcgagtatg ggaactcagt ctcatctgga atgacgggaa gcaatgccct ttcccccaat
     2881 gccccgtgcc caatctcacg ccgcccgggt ccgcgcatag gacgagtctc ccccacggag
     2941 aacggaggga agttgtagta gtgcatgtag tgcttcgtaa cctcttcctc gagactctcg
     3001 accagctggc cctctttctt gcctccgaga gtgacgatcg acagcacctg tgtctgacct
     3061 ctcgagaaca accccgagcc atggctcatg gggagaacgc caacgtcgac cccgatgggc
     3121 cggatctctt ccggggcacg ac
//
LOCUS       DATMZD010000135         4774 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_104423,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000135 DATMZD010000000
VERSION     DATMZD010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4774
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104423"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1811)
                     /locus_tag="VN478_02835"
     CDS             complement(<1..1811)
                     /locus_tag="VN478_02835"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HWQ21645.1"
                     /translation="MKSDTEFDVDKEYESLIAALRGWLSPEEIGLVDRAFVFARQRHG
                     DQKRASGAPYMLHPVKAALVLADYRLDAETYAACLLHDVLEDTNTNVQELVDGFGSKV
                     ASLVEGVTKLHGLNYLSREENNLENVRKILLAMAADLRVVLIKLADRLHNMRTLQYLS
                     EDKQHRIAQETMEIYVPLAHRLGIYELKWEMEDLAFRYLNPDEYRRLAGLVAAKRAER
                     EAFVKETINQTVHFLEEKDIHAQVSGRPKNLYSIYRKMVQQGKTFDEIYDLTAVRILV
                     NTIPECYLVLGYVHEAFTVVPGRVKDYIALPKPNGYQSLHTTVMGSNGEPLEIQIRTK
                     RMHEVDEMGVAAHWKYKEGKAADLVDSERFAWLRQMVDWQKDVRSSGEFVERVKVDVF
                     TDEVLVFTPKGDVINLPVGSTPVDFAYHIHTDVGNNLVGAKVNDRIVPIDTELVTGDR
                     VQVITSRNSGGPKSDWLKFVKAASTKGHIRAYLRKKELEHSPAERPEAEERPRVVVAR
                     PTFSERHAAAESGFHIVVRGDATLPIIMAACCNPSYPDEILGYVTRGRGITIHRVDCA
                     NIPSLTMEPERLVDAHWEMIPRKVRERTIVIETLNVPG"
     gene            complement(1808..2320)
                     /locus_tag="VN478_02840"
     CDS             complement(1808..2320)
                     /locus_tag="VN478_02840"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007724940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HWQ21646.1"
                     /translation="MNLRDFIRDIPDFPQKGIIFRDLTPLMGDADAFEFAVDELAKQF
                     RTDRIDKIVGVEARGFIIGAALSYALHVGFVPARKPGKLPYKSVRKEYELEYGVSILE
                     MHEDAIKPGERVLIVDDLLATGGTVSATADLVQSLGGDIVAYAFLVTLKDLGGASKLS
                     GGRVVSLLDL"
     gene            complement(2365..3291)
                     /gene="secF"
                     /locus_tag="VN478_02845"
     CDS             complement(2365..3291)
                     /gene="secF"
                     /locus_tag="VN478_02845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HWQ21647.1"
                     /translation="MTFRERILTWDIVGRTRTWFTASSIIIAIGVVAMIANVFSFGSP
                     LKFGIDFIGGTVINASFERAPDEAVVRDILSANGYSNVTVQRVGDKGITMKVEASEID
                     PAVSGKIINDIVAKYGAVDVGTKEITSIAPVIGRDLLRRSMLALGLSLLFTLLYITVR
                     FKFSFGLATILGMAHDIFVALGILAVFRVPLNAPFVGAFLAIVTYSVQDGVVVMDRIR
                     EKLRYRTKEPFDKLANAAVTETWMRSFNTSFTTFLAVLVLFIMGGAPIRDFSTTLLVG
                     IISGTYSSIYISTPLLVLWVQGREKAAALQKA"
     gene            complement(3303..4541)
                     /gene="secD"
                     /locus_tag="VN478_02850"
     CDS             complement(3303..4541)
                     /gene="secD"
                     /locus_tag="VN478_02850"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HWQ21648.1"
                     /translation="MRSKVMSSLILVIIVAIVAGVINYQGLIAKNTKLAPKLGLDLRG
                     GVRIVLEAQDTAGAKATSENIDSAVAVIDKRVNALGVSEATAVREGASSKRIIVELPG
                     WEDPEKAKEIIGKTALLEFKTEDGKTIVNGSNLKSAQLVFSQEQASLGEPQISFELDA
                     AGTAAFATATQANIGKTITIYLDSQVLMSPTVEVAITDGKGVISGGFTAEQASNYALL
                     LRSGSLPVKLVAISEEVVGPSLGNAFVRRSAWAAVIAFLLVVVWMFVMYRYLGLVSGI
                     ALVVYADILMAAYLVLHATLSLPAIGGAILSIGMAVDANCIIFERIREELGMKKTVKG
                     AIGAGFSRAFRAVFDSNLTVLVGTAFLFYFGTGTVRGFAVTLALGVLASMFTAVFASH
                     TLVDLLLTPAIARNSRKFLG"
     gene            complement(4627..4755)
                     /locus_tag="VN478_02855"
     CDS             complement(4627..4755)
                     /locus_tag="VN478_02855"
                     /inference="COORDINATES: protein motif:HMM:NF013835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21649.1"
                     /translation="MHNLQFLQTMIGEIRSAISAGTFAEYKRAFADRYTAASDRVW"
BASE COUNT         1054 a         1414 c         1295 g         1011 t
ORIGIN      
        1 ccagggacat tcagcgtctc gatgacgata gtccgttcgc gcacctttcg agggatcatc
       61 tcccagtgag cgtcaacaag acgctctggc tccatggtca gcgacggaat gttggcgcag
      121 tcgacccgat gtatcgtaat gcccctgccg cgcgtcacgt agccgaggat ctcatcggga
      181 taggacggat tgcagcacgc agccatgatg atggggagtg tcgcatctcc tcgaacgacg
      241 atgtggaatc cgctctccgc tgcagcgtgt cgttccgaga atgtcggccg agccacgacc
      301 actcgcggac gctcctctgc ctcgggcctc tcggcaggag aatgctcaag ctccttcttc
      361 ctcaggtagg cgcggatgtg tcccttcgtc gaggctgcct tcacgaactt cagccagtcg
      421 gacttcggtc ctccggagtt gcgcgacgtg atcacctgaa cacggtctcc ggtcaccaat
      481 tccgtgtcga tggggacgat acgatcgttc accttcgcac ccaccagatt gttccccacg
      541 tccgtatgga tgtggtaggc gaagtccacg ggtgtggaac caactggcaa gttgatgacg
      601 tcgccttttg gagtgaagac gaggacctcg tccgtgaaga cgtctacttt cacgcgctcc
      661 acgaactccc cactgctgcg aacatccttc tgccagtcta ccatctggcg gagccacgca
      721 aatcgctccg aatcgaccag atcggccgcc ttgccttcct tgtacttcca atgggcggcc
      781 acgcccatct cgtcgacctc gtgcattcgc ttggttcgta tctgaatctc cagcggttct
      841 ccgttggagc ccatcacagt agtgtgcagc gactgatagc cgttcggctt cggaagggca
      901 atgtagtcct tcaccctccc tgggacgacc gtgaatgcct cgtgaacgta tcccagaacg
      961 aggtaacact ccgggatcgt gttaacaaga atccgcactg cagtcaggtc atagatctcg
     1021 tcgaatgtct ttccctgctg gaccattttc cgatagatgc tgtacaggtt ctttggccgt
     1081 cccgagacct gtgcatggat gtccttctct tccaggaagt ggacagtctg gttgatagtc
     1141 tccttgacaa acgcctcccg ctcagcacgt ttggcggcaa ccagcccggc cagtctccgg
     1201 tactcgtcgg ggttcaggta gcggaaggca agatcctcca tctcccactt gagctcgtat
     1261 atcccgagcc ggtgggcgag aggtacgtag atctccatcg tttcctgtgc gatgcgatgc
     1321 tgcttgtcct cgctcagata ctgcagcgtc cgcatattgt gcagtcggtc agccagcttg
     1381 atcagcacca cccgcaaatc agcggccatg gccaggagga tcttgcggac attctcgagg
     1441 ttgttttcct cacgagagag gtagttgagt ccgtggagtt ttgtgacacc ctctaccaaa
     1501 gatgccacct tggaaccgaa cccgtcgacc agttcctgca cgttggtatt ggtgtcttcc
     1561 aacacatcgt gcagcagaca cgcggcatag gtctcggcgt ccaggcgata gtcggccaga
     1621 accagagctg ctttgaccgg gtggagcata tagggagcgc cagaagcacg tttctgatcc
     1681 ccatgcctct ggcgcgcaaa cacgaatgct cgatcaacga gaccgatctc ctcaggggag
     1741 agccagcctc gaagcgcagc tatgagactc tcatattcct tgtcaacgtc aaactcagtg
     1801 tcagacttca aaggtcaagc agtgagacaa cccttccacc actcagcttg ctggctccgc
     1861 cgaggtcctt cagagtcacc agaaatgcgt aggcaacaat gtccccaccc agtgattgaa
     1921 caagatcggc agtggcactg actgtaccac ccgtcgccag caggtcgtcg acgatgagca
     1981 cgcgctcgcc tggcttgatg gcgtcctcat gcatctccag aatcgagacc ccgtactcca
     2041 gctcgtattc ctttcgcacg gatttgtaag gaagcttgcc cggcttgcga gccggcacaa
     2101 aaccgacatg cagggcatag gacagggcgg cgccgatgat gaagcctctc gcttccactc
     2161 cgacgatctt gtcaatacgg tctgtcctga actgctttgc caactcatcg acggcaaact
     2221 cgaacgcgtc ggcatcaccc atgagtggcg tcagatccct gaatatgatt cccttctgag
     2281 gaaaatcggg tatgtcgcgt atgaagtctc tcaggttcat tttgcctcct gcctcgccct
     2341 gtgtcaccag gaaaccagga tatgctaggc cttctgcaga gcagcggcct tttctctacc
     2401 ctgcacccag agaaccagca gaggcgtaga gatgtagatg gaagagtagg ttccggagat
     2461 gatgcccacc agcagcgttg tcgagaagtc gcgaattggt gccccaccca ttatgaacaa
     2521 gaccagcacg gcgagaaacg tcgtgaagga cgtattgaat gaccgcatcc acgtttcggt
     2581 gactgcagcg ttcgcaagct tgtcgaaagg ttctttggta cgataccgca acttctcgcg
     2641 aattcggtcc atgacgacca cgccgtcctg caccgagtat gtgacgatcg ccaggaaggc
     2701 cccgacaaat ggcgcattga gaggcactcg gaagactgcc aggatgccga gagcaacgaa
     2761 gatgtcatga gccattccca gaatcgtcgc aagtccgaac gagaacttga agcgaacggt
     2821 aatatagagc agcgtgaaca gcagtgaaag gcccagtgcg agcatggaac gccgcaagag
     2881 gtcacggcca atcacaggtg cgattgacgt tatttccttg gtgccaacgt cgacagcacc
     2941 atatttggcc acgatgtcgt tgatgatctt gccgctgaca gccgggtcga tctcgctggc
     3001 ctcgaccttc atcgtaatgc ccttgtcgcc gacccgctga accgtgacgt tgctgtagcc
     3061 attggccgac aggatgtcgc ggactactgc ctcatcggga gctctctcaa aggaagcatt
     3121 gatgacggtc cctccgatga agtcgatgcc aaacttcaat ggagacccaa aactgaagac
     3181 attggcaatc attgctacga cgccaatcgc gatgataatg gacgacgcag tgaaccacgt
     3241 gcgggttcta ccaacgatat cccatgtcag aattctttcc ctgaatgtca tgactgcacc
     3301 gcctaaccga ggaatttcct gctgttcctg gcaatggcag gagtgagcag aagatcgacc
     3361 agcgtgtggc tggcaaacac agccgtgaac atgctggcga gaacaccgag agcaagggtt
     3421 acggcgaatc cgcgtacggt gccggtgccg aagtagaaca ggaacgcagt gcctacgagt
     3481 accgtcaggt tggagtcgaa cacggcacgg aaagcccgcg agaagccagc tccaatcgcg
     3541 cccttgacgg tcttcttcat cccgagttcc tctcgaatcc tctcaaagat gatgcagttg
     3601 gcatcgaccg ccatgccgat ggacagaatc gcacccccga ttgcaggcag cgacagagtt
     3661 gcatgcagca caagatatgc ggccatcaga atgtcggcat agacgacaag agcaatgccg
     3721 gagacaagac ccagatagcg atacatgacg aacatccaga caacgaccag aaggaatgcg
     3781 atcaccgccg cccaagcact gcgacgaacg aacgcgttgc cgagggaggg cccaaccacc
     3841 tcttcagaga tcgcaaccaa cttgaccggg agtgaaccgc tccgcagcaa cagcgcatag
     3901 ttgctggcct gttcagccgt gaaaccgccc gagataacgc ccttgccgtc ggtgatcgcg
     3961 acttcgactg tcgggctcat gaggacctgg ctatcaaggt agatggtgat cgtcttgccg
     4021 atatttgcct gcgtcgccgt ggcaaacgct gcagtaccgg ctgcatccag ctcaaacgag
     4081 atctggggtt ctcccagact cgcctgctcc tggctgaaga caagctgtgc agacttgagg
     4141 ttcgagccgt ttacgatggt cttgccatcc tccgtcttga attccaggag ggcggtcttg
     4201 ccgatgattt ccttggcttt ctcggggtct tcccagccgg ggagttccac gataatgcgc
     4261 ttcgagctcg caccctcgcg cacagcggtg gcctcgctta cgccaagcgc attgacacgc
     4321 ttgtcgatga cagcaacggc cgagtcgatg ttctcgctcg tcgccttggc gccggctgtg
     4381 tcctgagctt cgagcacaat gcgcacccca cctctgagat caagtcccag cttcggagcg
     4441 agcttcgtgt tcttggcaat cagcccctgg tagttgatca ctccagcgac aatcgccacg
     4501 ataatcacaa gaataaggct tgacatgact ttcgaccgca ttatggttcc tcctggtttc
     4561 gacaacactc taaatctaca tcaaatgaga ctcactgtca atgcggcaaa gcgcgctacg
     4621 agccccttac cacaccctgt cggatgctgc agtgtaccgg tcggcgaatg cgcgcttgta
     4681 ctcggcgaaa gtgcctgcag agatcgcgct tcggatttca ccgatcatcg tctgaaggaa
     4741 ctggaggttg tgaatggtgg cgagtgttcc cgca
//
LOCUS       DATMZD010000136         7588 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_84357,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000136 DATMZD010000000
VERSION     DATMZD010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7588
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84357"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VN478_02860"
     CDS             <1..183
                     /locus_tag="VN478_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21650.1"
                     /translation="FLSRVYELETQIYDNLDELETAFESPKDLGRRDTLKGIRKRLEV
                     ISQDFATRKETLKSAR"
     gene            281..2725
                     /locus_tag="VN478_02865"
     CDS             281..2725
                     /locus_tag="VN478_02865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HWQ21651.1"
                     /translation="MTTEHTASSRHRGRPRFKVVLSWLVVAVLAVGLAGSAVVALMFA
                     HYATEVPPVGSLFIETPKASQVFDRNDNLLSSVYFAENRLPKQLTEISEKAIQALIAS
                     EDERFFSHGAIDYRGLVTALIVDPLRGQGIRGASTLTQQLARQAILHDTDVTIARKFK
                     EMIVAFRLEEQYTKEEILNMYLNYVYFGSGLYGIEAAALGYFGIPSRDLDLAQAAMII
                     GVLPAPSAYSPYENLEKAEERRDAVLYKMVKNHYISQAEADAASAEKLTLRNGMKSPY
                     GSIGWFVDYVKSIVQDEYGDEMLYQGGLRIYTTIDPKLQEAALTAVQKVWDQAKKDKV
                     FVDSDRDSLGVFQPQVGLVSVDPKTGYILAMVGGTNYDETNFNRVLAPRQPGSTFKLF
                     DYTAALDNGATFPSEIVASQPTDFGGWNPPEWLGTNQWFGRISVANAIKESSNIIAAK
                     TTLRAGLDRVIYYARKMGVTSPLKPYVSLSIGSFEISPLEMVEAYSTIANGGKRITPY
                     PIRRIEAQDGTTIEAHNFAATQVVPASTAWLVQTMLRGVYENTANARISGLQAAGKTG
                     STNDWRDAWYDGFTPELCTVIHVGADAARAQMSAIRNAGSRFPAMTWKAYMQQAIKLE
                     KQTNFGPKPDGISVRTICDVSGLLASESCPEASTHSAYFLSSHVPTQSCTVHPPILQT
                     VRLAAEDTTKLAPSDWPDSRVVVRTMLRDQVPVEVYGQQTASDDQLSVTAFPNELKVG
                     QQVTLRLDASRAAGSAATSFDVYVGGTLVTSASSLPVTVSYVPTVQGTLEIAIVGRSP
                     ALDTVFQATSTVQVQP"
     gene            2967..4163
                     /locus_tag="VN478_02870"
     CDS             2967..4163
                     /locus_tag="VN478_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HWQ21652.1"
                     /translation="MTNQEVEALARRYEPAILEFARDIIKTRSYSTQEQDLVALIKAK
                     MEALDFDEVKVDGIGNIIGRVGSGPRKILYDAHIDTVWAEDKGNWKMDPFAAVVDGNL
                     LYGRGSSDEKAAMSSMVYGAAMAKQLGLLDGFSLYISGTVLEETCDGLSVIHMIEKEG
                     LRPDFVVICEPTSLDVYRGHRGRVELKITTTGTSAHAAHPDKGDNAIYKMARTVLNIE
                     KLTATFKDDPFLGKGTAVVSIIESKAPSINSVPYESTIYIDRRITVGETKETVLAEFR
                     GAVYDPEHTTVELTEFEDTSWTGLHVCQEKYFPTWVLDENHPLVRAGVSAAREARAKD
                     PTVSRWIFSTNGVAFMGRFHIPSIGFGPGDEWLAHAPNEYVRISDLYEAARFYALLPS
                     KLAKEV"
     gene            4169..5149
                     /locus_tag="VN478_02875"
     CDS             4169..5149
                     /locus_tag="VN478_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HWQ21653.1"
                     /translation="MNTRMKGRDFLSTNDWTTEELDMLLDVAFDLKKQKALGIPHHLL
                     EDKTLFMLFKDKSTRTRNSTEAAMTQLGGHAHYLTEEAVQISHGDTEKEMGIILSRYG
                     HGIAIRDDIYLGVGHKYMTNIAKYADIPVISLESDFDHPCQMMADIMTVKEKFHNEIR
                     GRKFVMSWAYAPSYAKPLACAQGSIMMMPRFGMDVTLAMPPEFSLQPEALGIARQNAQ
                     EAGVRFEVTNDMDAAMEGADIVEAKSWGCFMTTRDLKESQEIAKKYTSWIMDQRRMDL
                     ASDSAIYMHPLPADRDYEVTNAVIDGTHSVVYDEAENRLHTVKAILALTM"
     gene            5182..6132
                     /gene="arcC"
                     /locus_tag="VN478_02880"
     CDS             5182..6132
                     /gene="arcC"
                     /locus_tag="VN478_02880"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015892748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HWQ21654.1"
                     /translation="MSKLAVIAIGGNSLIRDESHRSVADQYDAVVETTHHIAGMVEQG
                     YDVVITHGNGPQVGFILLRSEIAEKVAGLHAVPLDSCGADTQGAIGYQIQQALHNEFL
                     RRGISRQAATVVTQVLVDRADPAFSNPSKPVGSFYSKEEIQDKVAREGWNVVEDAGRG
                     WRRVVASPMPIQVIELDAIRSLVKAGFVVVAAGGGGIPVIRDEDGNLKGVAAVIDKDH
                     ATSLLATNLSADLFIISTAVEKVYINYNKPDQRGLERMTIDEAKMYMDQGQFAKGSML
                     PKVKAAVAFLEHGGGEALITNPESLERAIAGETGTRIVHD"
     gene            6216..7457
                     /locus_tag="VN478_02885"
     CDS             6216..7457
                     /locus_tag="VN478_02885"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HWQ21655.1"
                     /translation="MSSVQKLRCVRCGCEYSPTDVRYVCPACGNDGILEVLYDYDRIR
                     RVLTKEKLQSDPDRSMWRYLAVLPVEERPASLTLQIGWTPLYASRRVGPQLGLDHVWF
                     KDDGRNPTASLKDRASAIAIAKAAELDERVLTAATTGNAGSSLAGLAANAGLTAYIFA
                     PASAPAGKIAQLRVFGSHLFLVDGNYDQAFDLCMEATERFGWYNRNTAFNPYMVEGKK
                     TVALEIAEQLHFEVPDKVIVPVGDGCIVSGVAKGFRDLLRLGLVDRVPQLIAVQAEGC
                     APIARAVMSGDPVAYLDDPTSCADSIVAGIPRNHLMAVRDIRSSGGLAVTVSDDEIID
                     AIGFLGRNEGVFAEPAAAASVAALRKLASSGDLHQKDRIAVLITGNGLKDVQNALKVK
                     GTSMAISPHITAVEQAIAQGF"
BASE COUNT         1705 a         2246 c         2203 g         1434 t
ORIGIN      
        1 ttcctgtcca gggtctatga actggagaca cagatatacg acaatctcga tgaactcgag
       61 actgcattcg agtcgccgaa agacctgggc cgtcgagaca cgctgaaagg catccgcaag
      121 cgtctcgaag tgatctctca ggacttcgca actcgcaagg aaacgctcaa gtcagctcgg
      181 tagcgcagcg cacgtcccga atcccgacgc aggcggcagc atctgccgtc gacggagtct
      241 ggggcggtgt gactccgtac cgacaacctg gaggtattgc ttgactacag aacacacagc
      301 ctcatccaga catcgtggta gaccgcggtt caaggtcgta ctcagttggc tggtcgtggc
      361 agtacttgcg gttggacttg ccgggagcgc cgtcgtggca ttgatgtttg ctcactacgc
      421 caccgaggtg cctcctgtcg gttcactgtt cattgaaaca ccaaaggcct cacaagtgtt
      481 cgaccgcaac gacaaccttc tttcatccgt atactttgcg gagaaccggc tgcccaagca
      541 actcacggag atctcagaaa aggccatcca agcactgatt gcctctgaag atgaacgctt
      601 cttcagccac ggtgccatcg actatcgcgg tctcgtcacg gccctgatcg tggatcccct
      661 ccgtgggcag ggcatccgcg gagccagcac tctgacgcaa cagctcgcgc ggcaggcgat
      721 cctacacgac actgatgtga cgattgcccg caagttcaag gaaatgattg tcgcgtttcg
      781 ccttgaggaa cagtacacga aagaggagat cctcaacatg tacctcaact atgtgtactt
      841 tggaagcggg ctgtatggaa tcgaagctgc agctctcgga tacttcggca taccatcccg
      901 tgaccttgac cttgctcagg ccgccatgat catcggagta ctgccggcac cgtctgcgta
      961 cagcccctac gagaatctgg agaaggcaga agagcggcga gatgccgtgc tgtacaagat
     1021 ggtgaagaac cactacatct cgcaagcgga agcagacgcc gcctctgcag agaaactcac
     1081 gctcagaaac ggcatgaagt ctccctatgg gagcattgga tggttcgtag actacgtcaa
     1141 atccatcgtt caggatgagt acggcgatga gatgctctac caaggtggac tgcgcatcta
     1201 cacgacgatc gacccgaaac tccaggaagc ggccctcacg gccgtccaga aggtctggga
     1261 ccaggcgaag aaagacaagg tgttcgtgga tagcgaccgc gattcgcttg gcgtcttcca
     1321 gccccaggtc ggcttggtca gtgtcgatcc gaagaccggc tacattctcg ccatggtggg
     1381 cgggaccaac tatgacgaga cgaacttcaa ccgtgtcctg gcgcccagac aacccggctc
     1441 gacgttcaag ctcttcgact acacggcagc actcgacaac ggggcgacct ttccttccga
     1501 gattgtcgct tcgcaaccta cggattttgg cggatggaat cccccggagt ggctgggaac
     1561 caaccagtgg ttcggccgaa tctccgtggc caacgccatc aaggagtcgt cgaatatcat
     1621 cgctgccaag acaacactca gagcggggct ggacagagtc atctactatg cacggaagat
     1681 gggagtcacc tcgcccctca aaccgtatgt ctccttgtcc ataggcagct tcgagatctc
     1741 gcccctggag atggttgagg catactcgac gatcgccaac ggaggaaaac ggatcacacc
     1801 gtatccgatc cggcgcatcg aggcacagga cggcacgacg atcgaggctc acaacttcgc
     1861 cgcgacccag gttgtgcccg cctctacggc ctggctcgtt cagacaatgc tgagaggcgt
     1921 atacgagaac acggcgaatg cacgcatcag cgggctgcaa gccgcgggca agaccggcag
     1981 caccaacgat tggcgcgacg catggtatga tggtttcacc ccggagctgt gcaccgtgat
     2041 ccacgtggga gctgacgcag cccgtgccca gatgtcggcc atacggaacg caggatcacg
     2101 gtttccagcc atgacatgga aagcctacat gcagcaggca atcaagctcg agaagcaaac
     2161 gaactttggc ccaaagcctg atggaatctc agtacgcaca atatgtgacg tatcaggtct
     2221 gctcgccagc gagtcctgtc ccgaggcctc cactcactct gcctactttc tgtcgagcca
     2281 cgtgccgacg cagtcatgca ctgtccaccc accgattctc cagaccgtac gactcgcggc
     2341 tgaggacacg accaagctgg ccccttctga ctggcctgac agtagagtcg tcgtcagaac
     2401 aatgctccga gaccaggttc ccgtcgaggt ctatggacag cagaccgcat ctgacgacca
     2461 gctcagcgtg acagcatttc cgaacgaatt gaaggtcgga caacaggtca cgctgcgcct
     2521 cgatgcgtcc cgggcggcgg ggtcagcggc cacatcgttt gacgtatacg tcggaggaac
     2581 gctcgtcacg tcggcttcat cactaccggt gacggtgtcc tacgtgccga ctgtgcaggg
     2641 cactctagag attgcgatcg tgggacgatc tcctgctctt gatactgtct ttcaggcaac
     2701 ctcgacggtc caggtgcaac cgtagcgtca ggacctcctg ctcaggacaa aggttctctc
     2761 cagacccggg cttccactgc gctacatgtg ctgggcgaac ggctgagagc tttattgctg
     2821 attgcattgg ctgccgtgtg gagtaaactg acagcattca ggattccatc cagcacaggg
     2881 gaaaggagta cgttcgacga tcctccgatt ggcttggccg cgttagggcc tgaccccttt
     2941 cggttgtcac ctgtcgagca accagcatga cgaatcagga agttgaagcg ttggcacgca
     3001 ggtatgaacc ggcgatcctg gagttcgccc gcgatatcat caagacgcgc agctattcga
     3061 ctcaggagca ggatctggtc gcgctcatca aggcgaagat ggaggctctc gatttcgacg
     3121 aagtgaaggt cgatggcatc gggaacatca tcggacgagt ggggagcgga cctcgcaaga
     3181 tcctctatga cgcacacatc gacacggtct gggcagagga caagggcaac tggaagatgg
     3241 atccttttgc tgccgtcgtc gatggcaacc tcctctacgg tcgaggatcc agtgacgaga
     3301 aggcagccat gtcatccatg gtgtacggcg ccgcaatggc aaaacagctg ggtctgctgg
     3361 acggattttc gctctacatt tctggaaccg tcctggaaga gacctgcgac ggactgtcgg
     3421 tcatccacat gatcgagaag gaggggctca gacccgactt cgtggtcatc tgcgagccga
     3481 cgagcctgga cgtctaccgc ggacaccgtg ggcgcgtcga gctcaagatc acgacgacag
     3541 ggacgtcagc ccatgccgcc catccggaca agggcgacaa tgcgatctac aagatggccc
     3601 gaaccgtact caacatcgag aagctcacag cgaccttcaa ggacgatcct ttccttggca
     3661 aggggacagc agttgtcagc atcatcgaga gcaaggcgcc ctcgatcaac tcggttccgt
     3721 atgagagcac gatctacatt gatcgacgca tcacggtcgg cgagacgaag gagactgtcc
     3781 tcgcagagtt ccgtggagca gtctatgatc ctgagcatac taccgtcgaa ttgacggagt
     3841 tcgaggatac cagctggact ggacttcacg tctgtcagga gaagtacttc cccacgtggg
     3901 tgctcgatga gaaccatccg ttagtgagag ccggcgtgag tgccgccagg gaagcgcgag
     3961 ccaaggaccc cacggtatcc cgctggatct tctctacgaa tggcgtcgct ttcatgggtc
     4021 gcttccacat tcccagcatt ggattcggtc ccggtgacga gtggctcgcc cacgcgccca
     4081 atgagtatgt ccgcattagc gatctctacg aagcggcacg tttctacgca ctgctcccat
     4141 cgaagctcgc caaggaggtt tgagctctat gaacaccagg atgaagggaa gagacttcct
     4201 ttccaccaat gactggacca cagaagaact tgacatgctt ctggacgtcg cgtttgacct
     4261 caagaagcag aaggccctgg gcattcccca ccatctgctt gaggacaaga cgctgttcat
     4321 gctcttcaag gacaagtcca ctcggacccg caactccacg gaagcggcca tgacccagct
     4381 tggtggtcac gcgcattacc tgaccgaaga agccgtccag atctcgcatg gcgacacgga
     4441 gaaggagatg ggcatcatcc tgtcccgcta cggccacggc atcgccatcc gcgatgacat
     4501 ctacctcggc gtgggccaca agtacatgac caacattgcc aagtacgcag atatcccggt
     4561 catctcgctc gaatcggatt tcgaccatcc gtgccagatg atggccgaca tcatgaccgt
     4621 caaagagaag tttcacaatg agattcgggg caggaagttc gtcatgtcct gggcgtatgc
     4681 tccaagctat gcgaaacctc tcgcctgcgc gcaggggtcg atcatgatga tgccgcgatt
     4741 cggcatggat gtcacactgg ccatgccgcc tgagttctca ctgcagccgg aagctctcgg
     4801 catcgctcgc cagaacgccc aggaagccgg cgtcaggttc gaggtcacga atgacatgga
     4861 cgcggccatg gaaggcgccg acatcgtcga ggccaagtcc tggggatgct tcatgaccac
     4921 cagagacctg aaggagtcac aggaaatcgc gaagaagtac accagctgga tcatggatca
     4981 gcgccgcatg gatctggcca gtgactcggc catctacatg catcctctgc ctgccgaccg
     5041 cgactacgaa gtgacgaatg cagtcatcga cgggacccac tctgtcgtat acgacgaggc
     5101 agagaatcgg ctgcataccg tcaaggcgat tctcgccctc accatgtagc tcgcggacac
     5161 tcgcgaagga gccctcgaga catgtccaag ctagctgtca ttgcaatcgg aggcaattcg
     5221 ctgatcaggg acgaatccca caggagcgtt gctgaccagt acgatgcagt cgtagagaca
     5281 acacaccata tcgcaggcat ggtcgagcag gggtatgatg ttgtcattac tcatggcaac
     5341 ggcccccaag tgggcttcat cctccttcgc tcagaaatcg cggagaaagt cgcaggcctg
     5401 catgccgttc cactggactc ctgcggagcc gacacccagg gagcgatcgg ataccagatc
     5461 cagcaagctc tgcacaacga attcctgaga agaggtatct ccagacaggc cgccactgtc
     5521 gtgacgcagg tccttgtcga ccgtgccgac cctgctttca gcaacccgtc caagccggtg
     5581 gggtccttct acagcaagga ggaaatccag gacaaggtgg ccagagaagg ctggaacgtg
     5641 gtcgaggacg ccggccgagg ctggcgccgg gtggtagcat ctcccatgcc gatccaggtc
     5701 atcgagctgg acgctatacg gagtctcgtc aaggccggct tcgtcgtcgt cgctgccggc
     5761 ggcggcggga tccccgtcat ccgggatgaa gatggcaacc tcaagggcgt cgcagcggtc
     5821 atcgacaagg atcatgcgac gagcctcctg gcaaccaatc tcagcgcaga cctgttcatt
     5881 atcagtaccg ctgtggagaa ggtatacatc aactataaca agccagacca gcgagggctg
     5941 gagcgcatga ccatcgacga ggccaagatg tacatggatc agggtcagtt tgcaaaaggg
     6001 tcgatgctcc ccaaggtgaa ggcggccgtg gcattcctcg aacacggcgg cggggaagcc
     6061 ctcatcacta atcctgagtc gctcgaacgg gctatcgccg gcgagaccgg caccaggatc
     6121 gtgcacgact gatccaagga ctctcatcga cgatacatga tgtctgggcg ggtgagccac
     6181 agttccttca cccgccttct cggtaaggag gacagatgtc ttcggtccag aaactgagat
     6241 gcgtgagatg cgggtgcgag tactccccga ctgacgtccg ctatgtgtgt cctgcctgtg
     6301 gcaatgacgg gatcctcgag gtcctctacg actacgaccg gatccggaga gtactcacga
     6361 aggagaagct gcaaagtgat cccgaccgct cgatgtggcg ataccttgcg gtgctgcctg
     6421 tggaggagcg tcctgcgtcg ctgacactcc agatcggctg gacgccgctg tatgcaagcc
     6481 ggcgcgtagg tcctcaactc gggctggacc acgtgtggtt caaggacgat gggcgcaacc
     6541 cgacagcgtc actgaaggac agggcttctg cgatcgccat tgccaaggcg gccgaactcg
     6601 acgaacgcgt cctcaccgcc gcgacgacgg gaaatgcagg ctccagcctg gcggggctcg
     6661 ctgcgaatgc aggactcacc gcctacatct tcgcgccggc gtccgcgccg gcgggaaaga
     6721 tagcccagct gcgagtattc ggcagccacc tctttctcgt cgacggcaac tacgaccagg
     6781 cattcgacct gtgtatggag gcaaccgagc gatttggctg gtacaacaga aacaccgcct
     6841 tcaacccgta catggtcgag ggcaagaaga ctgtggccct tgagatcgcg gagcagctgc
     6901 acttcgaagt gccagacaag gtgatcgtac cggtcggaga tggctgcatc gtctctggtg
     6961 tcgcaaaggg attccgtgac ctgctgaggc ttgggctcgt cgaccgggtg ccacagctca
     7021 tcgccgtgca ggctgagggc tgtgctccaa ttgcgcgtgc agtcatgtcc ggcgacccgg
     7081 tggcttacct ggacgacccc acgtcctgcg ctgacagcat cgtcgcaggc attccgcgca
     7141 accacctcat ggctgtccgc gacatccgat cgagtggcgg cctggccgtt accgtgtccg
     7201 acgatgagat cattgacgcc atagggttcc tcggccggaa cgaaggtgtc ttcgccgaac
     7261 cagccgcagc ggcttccgtc gccgcgctgc gcaagcttgc cagcagcggg gacctgcacc
     7321 agaaggatcg catcgccgtc ctgatcaccg gcaacgggct gaaggacgtc cagaatgctc
     7381 tgaaggtgaa gggtacgagc atggcgatct caccgcacat caccgctgtg gaacaggcga
     7441 ttgcacaagg attctgaccc tctgttcgtc ctcgatccga gccactcatg aatggtcctg
     7501 cacatgggcg cgttgctttt tgaacgctgg acagtagaat tcaaccgaaa actctagcta
     7561 ttgggaggag atgccacaat gctgagcc
//
LOCUS       DATMZD010000137         2951 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_54350,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000137 DATMZD010000000
VERSION     DATMZD010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54350"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            372..446
                     /locus_tag="VN478_02890"
     tRNA            372..446
                     /locus_tag="VN478_02890"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:404..406,aa:Val,seq:gac)
     gene            470..2395
                     /gene="thrS"
                     /locus_tag="VN478_02895"
     CDS             470..2395
                     /gene="thrS"
                     /locus_tag="VN478_02895"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HWQ21656.1"
                     /translation="MDIRIVFGDTSSQYSFTDGESILEAVQKLLPQQKRSIVAARVGG
                     SLVDLTTIPSTADDIQLVSTASAEGLEILRHSTSHIMAEAIKELYPDAKLAIGPSIDS
                     GFYYDIDMPKTLSPDDLGAIEQKMREITERSEHFTRQDKPIAEAIEFFRERGDQYKVE
                     ILSEIPATTVSLYTQGAFTDLCRGPHIPSTSYLKHFKLLSIAGAYWRGDEKNKMLQRI
                     YGTAFPSKEELAGYVTAREEAAKRDHRKLGQQLDLFSIQEQAGAGLVFWHPKGAIVRK
                     EIEDFWRAEHIKRGYEFVYSPHIANLELWKTSGHWGFYQQNMYSPIQVDEDQYMLKPM
                     NCPFHILIYKSQVRSYRDLPLRWAELGTVYRYEKSGVLHGLLRVRGFTQDDAHLFIRP
                     DQINDEVKGVLDFTRHMIETFGFTKYNIYLSTRPEHSVGTDEGWQMATEALRVALEEM
                     HLEYKVDPGEGVFYGPKIDIKLVDAIGREWQGPTVQVDFNLPERFDMTYIGEDGKEHR
                     PIMLHRVVLGSMERFFGTLIEQYAGAFPLWLAPVQVEVVPITDQQEEYAKAVAARLLE
                     RGIRAKVDIRREKMQAKIRDAEMQKVPYIAVIGPKEAAVDSIAVRMRHEGDQGVVTVD
                     EFIDRVLEEIRTKVIFA"
     gene            2618..>2951
                     /gene="infC"
                     /locus_tag="VN478_02900"
     CDS             2618..>2951
                     /gene="infC"
                     /locus_tag="VN478_02900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HWQ21657.1"
                     /translation="MRWKEVRVIDENNGQLGLLTSEVALELAYSKGLDLIVVAPNATP
                     PVCKIMDLGKYQYEQMKRQKEARKKQVTVEMKEMKYRLRIDVGDLTVKNQHLKEFLSA
                     GNRVRVTIM"
BASE COUNT          673 a          786 c          872 g          620 t
ORIGIN      
        1 cagaatgggc gtgatcttgt tgaccatgac atccgtgcac tgagcgctga ggatggttgc
       61 cgtcaggagc tgccatggat cgcagaactc caaagcggta cccctcacgg ggtatgtctt
      121 ctcgagcgta gtgacgacct gtcgaacatg cttctgcggc gtgaatggca tctccatgcc
      181 tcctcacttg acgtctggat gcaagcaaca gtatactcgt atacggcgtt ggcagactgg
      241 cctgtacagc gggcttgcca aaaggcttcg cgagcggagg gcctctcgga ttgtcgtgtt
      301 cgggcgactc tcgcccaaag gccttgcagc cgggcgcgtt ctgcgtatac tacagcaagt
      361 ccggtttttg tgggcggtta gttcagaggt agaacgcctc gttgacaccg aggaggtcag
      421 aggtccgatt cctctatcgc ccaccatttg tatcttccag ggagcggaca tggatatcag
      481 aattgtcttt ggcgatacgt cgtcccagta ctccttcact gatggcgagt ccatcctcga
      541 ggctgtccag aagttgttgc cccagcagaa gcggtccatc gtcgctgcac gggtgggcgg
      601 gtcacttgtc gacctgacca cgattccgtc cacggccgac gacatccagc ttgtgagcac
      661 cgccagcgca gaggggcttg agatccttcg gcacagcacg tcccacatca tggccgaggc
      721 aatcaaggaa ctctatccgg atgccaagct tgcgatcggg ccctctatcg acagtggttt
      781 ctactatgac atcgacatgc cgaagacact gtcgccagat gatctgggcg ccatagagca
      841 gaagatgcga gagatcactg agcgcagtga acacttcacg cgccaggaca agcccatagc
      901 ggaagccatc gagttcttca gggagcgcgg tgaccagtac aaggtcgaga tcctgtctga
      961 gattcctgcc acaactgtgt ctctctatac gcagggagcg ttcacggatc tctgtcgtgg
     1021 gccccacatt ccgtcgacca gctatctcaa gcacttcaag cttctgtcga tcgcaggtgc
     1081 gtactggcga ggcgatgaga agaacaagat gctccagcgc atctacggga cagcgttccc
     1141 ttcgaaggaa gagctggccg ggtatgtcac agcgcgggaa gaagccgcga agcgggacca
     1201 ccggaagctg ggccaacaac tggatctgtt cagcattcag gagcaggctg gtgcgggtct
     1261 ggttttctgg catcccaagg gagctatcgt ccgcaaggag atcgaggact tctggcgtgc
     1321 cgagcacatc aagcgcggat atgagtttgt ctactcgcct cacatcgcga acctcgagct
     1381 ctggaagaca tccggccact ggggcttcta ccagcagaac atgtactctc cgatccaggt
     1441 cgatgaggat cagtacatgc tcaaacccat gaactgcccg tttcacatcc tcatctacaa
     1501 gtcgcaggtc cggagctatc gtgacctccc gctgaggtgg gctgaactcg gtacagtgta
     1561 tcgctatgag aagtccggcg tgcttcacgg tctcttgcgc gtgcgaggat tcacgcagga
     1621 cgatgcccac ctcttcatcc gtccggacca gatcaatgat gaggtcaagg gagtccttga
     1681 tttcactcgt cacatgatcg agacgttcgg gttcacgaag tacaacatct atctcagtac
     1741 gcgtccagag cactccgtcg gaacggatga aggatggcaa atggccaccg aagctctgag
     1801 agtcgcactg gaagagatgc atctcgagta caaggtcgac ccgggcgagg gggtcttcta
     1861 tgggcccaag atcgacatca agctcgtcga tgccatcggt cgtgaatggc agggaccaac
     1921 tgtgcaggtt gatttcaacc tccccgagcg cttcgacatg acgtacatcg gcgaagatgg
     1981 caaggaacat cgtccaatca tgctgcaccg cgtggtactg ggctccatgg aacgcttctt
     2041 tgggacgctc atcgagcaat atgcaggcgc gttcccactc tggctcgctc ctgttcaggt
     2101 ggaggtagtc cccatcacag atcagcagga ggagtatgcg aaggcagttg ctgcaaggct
     2161 gctggagcgt ggaatccgtg ccaaggtgga tatccgcagg gagaagatgc aggcgaagat
     2221 ccgtgatgcc gagatgcaga aggtgcctta cattgcagtc ataggtccca aggaagcagc
     2281 ggtagattcg atagctgtgc ggatgcgcca cgagggtgac cagggtgtcg tgacggtcga
     2341 cgagttcatt gaccgggtcc tggaggaaat caggaccaag gtcatcttcg cctagttgat
     2401 tggcaagaga acactgctat acttgtcacg gctcagaaga gtcgagccac tgtaaaacga
     2461 agccagtatg gcttctacct ccagcctagt gcaaagaggt ccttgagttc gccagattgt
     2521 gtatgcgact acatggcggc cgttttacag cggccgccat ttttcttttt ggaggtgtgg
     2581 aatcaatagg gattcagatc tgatttacaa cgagagagtg cgctggaagg aagtccgcgt
     2641 cattgacgag aacaacggcc agttgggcct gttgacgtcg gaagtggcac ttgaactcgc
     2701 gtactcgaag gggctcgacc tgatcgtcgt ggcgcccaac gccactccac ccgtttgcaa
     2761 gatcatggac ttgggaaagt accaatacga gcagatgaag cggcagaagg aagccaggaa
     2821 gaagcaggtc acagtcgaga tgaaggaaat gaagtatcga ctgcgcatcg atgttggcga
     2881 tctgaccgtg aagaatcaac acctcaagga attcctgtct gcggggaacc gcgtgcgtgt
     2941 cacgataatg t
//
LOCUS       DATMZD010000138         4610 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_84479,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000138 DATMZD010000000
VERSION     DATMZD010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4610
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84479"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VN478_02905"
     CDS             <1..325
                     /locus_tag="VN478_02905"
                     /inference="COORDINATES: protein motif:HMM:NF014886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate kinase alpha/beta domain-containing
                     protein"
                     /protein_id="HWQ21658.1"
                     /translation="AQTVGAKLIVCATESGRTSILISHYRPRTPILALTPSDLVQRRL
                     ALYYGIIPMVVQQFESVDHILRTAVAMSQVSHLAHRGDKIVITAGSHAGVAGSTNLIK
                     VEELE"
     gene            423..1784
                     /locus_tag="VN478_02910"
     CDS             423..1784
                     /locus_tag="VN478_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018962608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWQ21659.1"
                     /translation="MKRKASYIKIFLLGFGFFGISVLWSLYNSDIPILLKNVYNLPYT
                     ASGWVMNIDNILAITLIPLVGFWSDHIWTRIGRRMPFIISTLPVGAILFALVPWIPLW
                     LGSNATSLLSFVLVLFFVNICMGISRSPIIALMPDLVPPEERSPANGVINFMGGFGSL
                     LVYFVVGKISTTNRPLGFAIAGASVLIAVIIMFLSINESRDSLDRKAPQSERADFSLL
                     RRMWTPKYRSLMLVCLAIFFWFIAYNAIETYYPTYMYNESPAVKTLITKLGTATPTDP
                     VAIAQLEKARQASESSAKTNLGIFSVAFMVLAIPAGYIGKALGRKTTILIGLVGVTVM
                     VLLLAFGIANTTGLAVFLLGGLSWALVNINSLPTVLDMGDLEMVGALTGVYYFFSQAA
                     SIVSPPLVGRIADLAGDTMSVMFPYAGIFFVLAAACMLFMNPKDRPAVASGQTPSANA
                     PAV"
     gene            1768..2535
                     /locus_tag="VN478_02915"
     CDS             1768..2535
                     /locus_tag="VN478_02915"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWQ21660.1"
                     /translation="MPLPFDKASAAPIRTQAGSDTACLLLHGLTGAPARDLWFLADRL
                     SAHGISVVAPLLTGHGRTWKELEAATAESWQDDALAGLDEARAMGKTVFVVGLSMGGT
                     LTLYLGEHRPEITGLVTINAPVYVDDPRMKVVPILRHFRKSQPKGPPDVADRDAMLPD
                     LGFNSLEAVYQFERLIGGVRRDLGMVTQPLVSFRSTQDHVVPPRNAEYIQDHVNSRFK
                     SIVTLEHSYHCATIDYDRERIATGIEQFITLVQEQHV"
     gene            complement(2613..3950)
                     /locus_tag="VN478_02920"
     CDS             complement(2613..3950)
                     /locus_tag="VN478_02920"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWQ21661.1"
                     /translation="MRRFFVVLKKELREMLTWQTLIPILLVMVIYSFLGRTINQEQKK
                     QAAPQPITVVDLDHTQTSELLSGILTKANFEVTTVTEGVVDDIVRKVASEKGSAVLVI
                     PPGFEAGIQAQHPQKVQTYTIMRNFSMMGIQGTFKMDAALSAMNEYIGDMYIKSGAPG
                     LDPAVVKHAISAQSFLTVGTTSAEGNAQEIVSVVMQQTMIIPIVLFLVIIMAAQMIAA
                     SIAGEKENKTLETLLSTPVARGALVTAKMMASGIVALVSAGAYMVGMRSYMGSLIGGA
                     SGTSAAAGQAIEKLGLVLDARGYVLLGLSVFAGIMVALSIAVILGAFAEDLKSVQIII
                     TPLMMVVLLPYLVTMFVDINSASPVLKWLMYAIPFSHPFLAAQFIFAHNFTMVIAGII
                     YEFALFFVLAAVAARIFSTDRILTMKLNTARRGASLGGWTLQREGISLRLRRK"
     gene            complement(3947..>4610)
                     /locus_tag="VN478_02925"
     CDS             complement(3947..>4610)
                     /locus_tag="VN478_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019680091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWQ21662.1"
                     /translation="GVSFEVAPGEIFALIGPNGAGKTTTLRIVATILQPTSGTARVYG
                     MDVQKDAAQIREVISYLPEEAGAYKNLTGLGYLNFMAELFASDVETQREYVQRASDIT
                     GLGERLRDKIGTYSKGMTRKLLLSRAVMTHPKLAIIDEPTSGLDVVAAIDIRESIRRT
                     TAEGMAVLLSSHNMLEIEYLSDRVAIVDKGTIHDIGTPQGLKDKYGKANLEEVFVQVV
                     KQ"
BASE COUNT          899 a         1470 c         1288 g          953 t
ORIGIN      
        1 ggctcagact gtcggggcaa aacttatcgt ctgtgcgacg gagtcaggca ggacctcgat
       61 cctgatctcg cactaccgcc cccgcacgcc cattctcgct ctcaccccga gcgacctggt
      121 ccagcgccgc cttgcgttgt actacggcat catcccgatg gtcgtccagc agtttgagag
      181 cgtcgaccac atcctccgca ccgccgttgc catgtcccag gtgtcgcacc tggcacaccg
      241 cggtgacaag atcgtcatta ccgcgggatc ccacgccggt gtcgccggca gcacgaacct
      301 catcaaggtc gaggagctgg agtaggcgct cgctgagcat tgcaaatcga gcagtacgca
      361 tagaatgacc ttggcacagt accccgggaa accggggtat gtcataacaa gggggaacag
      421 gcatgaaacg aaaggcatcc tacatcaaga tcttcctgct tggattcggc ttcttcggca
      481 tctcggtcct ctggtcgctc tacaactcgg acatccccat cctgctgaag aatgtctaca
      541 atctgccata cactgcgtcc ggatgggtga tgaacatcga caacatcctg gcgatcactc
      601 tcatcccgct ggtcggcttc tggtcagacc acatctggac gcgcatcggg aggcgcatgc
      661 cgttcatcat ctcgacattg cccgtgggag ccattctgtt cgccctggtc ccatggatcc
      721 ccctctggct cggctcgaat gcaacatccc tgctgtcctt cgtccttgtg ctgttcttcg
      781 tgaatatctg tatgggaatc tcgcgatctc ccatcattgc gttgatgccc gacctcgttc
      841 cgccggaaga acgctcgccg gccaacggcg tcatcaattt catgggcgga ttcgggtcac
      901 ttctagtgta cttcgtcgtc ggcaagatct ccacaaccaa cagaccgctg ggctttgcca
      961 ttgcaggtgc gtcagtcctg atagcagtca tcatcatgtt cctgtccatc aacgagtcgc
     1021 gtgactcgct tgatcgcaag gccccgcagt cagagcgcgc cgacttctcc ctgctccgca
     1081 ggatgtggac gcccaagtat cggagcctca tgctcgtctg cctggccatc ttcttctggt
     1141 tcatcgccta caatgcgatc gaaacgtact acccaaccta catgtacaac gagagccctg
     1201 cggtcaagac gctgatcacc aagctcggga cagcgacgcc gacggatcct gtcgccatcg
     1261 cacaactgga gaaggctcgc caggcctccg aatcgagcgc caagaccaat ctgggcatct
     1321 tctcggtagc cttcatggtc ctggctatcc ctgcggggta catcggcaag gccctgggac
     1381 ggaagaccac gatcctgatc ggcctggtcg gcgtcaccgt catggtcctg ctgctggcat
     1441 tcggcattgc caatacgaca gggctcgcgg tcttcctgct gggaggcctg tcctgggcgc
     1501 tcgtcaacat caactcgctg ccaaccgtcc tcgacatggg agacctcgag atggtcgggg
     1561 cactcaccgg cgtctactac ttcttctccc aggctgccag catcgtcagc cccccactcg
     1621 tcggtcgcat cgcagacctg gcaggcgaca cgatgtccgt catgttcccc tatgccggca
     1681 tcttcttcgt actcgcagcg gcctgtatgc tgttcatgaa ccccaaagac agaccggcag
     1741 ttgcctccgg gcagactccc tctgccaatg cccctgccgt ttgacaaggc ttcggcagcg
     1801 ccgatacgca cgcaggcagg ctcggacact gcctgcctgc tgctccacgg cctgaccggc
     1861 gcgcctgccc gggacctgtg gtttctcgca gatcgcctct ctgcgcacgg catttccgtc
     1921 gtcgcccctc tccttacggg gcacggcagg acgtggaagg agctggaggc cgcaactgcc
     1981 gagtcctggc aggatgacgc cctcgccggg ctcgacgaag cccgtgccat gggcaagacc
     2041 gtgttcgtcg tcggattgtc gatgggcggg accctgacgc tgtaccttgg tgaacatcga
     2101 cccgagatca cggggctcgt aacaatcaac gcgcctgtct acgtggacga tcccaggatg
     2161 aaggtcgtcc cgatactgcg gcacttccgg aagagccaac ccaagggacc acccgacgtc
     2221 gccgatcggg acgccatgct gccggatctc gggttcaact cgctggaagc cgtctatcag
     2281 ttcgaacgcc tcataggagg agtgcgtcga gacctcggca tggtgactca gcccctggtc
     2341 tccttccgct cgacacagga ccatgtcgtt ccaccccgca acgccgagta cattcaggat
     2401 cacgtcaaca gccgcttcaa gagcatcgtc accctggaac actcctacca ttgtgccacc
     2461 atcgactatg accgcgagag gatcgccacc ggcatcgaac agttcatcac gctggtacaa
     2521 gagcagcacg tctgagactt cgtccctgcg ttgcatgata tgacaacccc cggccgtgag
     2581 gccgggggtt ctgacatgcc agaaggcagg tgctacttgc gccgcaaacg aagactgatg
     2641 ccttcccgct ggagcgtcca gccgcccagg cttgcgccgc gccgcgccgt attgagcttc
     2701 atggtcagga tgcggtcggt cgagaagatg cgggctgcaa ccgcagccag aacgaagaac
     2761 agcgcgaact catagatgat gccggcgatg accatcgtga agttgtgggc aaagatgaac
     2821 tgggctgcca gaaatgggtg tgagaacggg atcgcgtaca tgagccactt caagaccggc
     2881 gacgccgagt tgatgtcgac aaacatcgtg acgagatagg gcagcaatac gaccatcatg
     2941 agtggtgtga tgatgatctg cacgctcttg aggtcttcgg cgaaggcccc caggatgact
     3001 gcaatggaga gagccaccat gatccctgcg aatacgctca agccaaggag cacgtacccg
     3061 cgcgcgtcca ggaccagtcc cagcttctcg atggcctggc cggcagctgc agacgtgccg
     3121 ctggcaccac cgatgagact acccatgtag ctcctcatac cgaccatgta tgcgccggct
     3181 gagaccagcg cgacgatgcc ggaggccatc atcttggccg tcaccagggc accacgtgcg
     3241 acgggcgtgg agagaagcgt ctcaagcgtc ttgttctcct tctcgcccgc gattgaggct
     3301 gcgatcatct gagcggccat gataatcacc aggaagagca cgatcgggat gatcatggtc
     3361 tgctgcatga cgacactcac gatctcctga gcattgccct cggccgacgt tgtcccgacg
     3421 gtgaggaacg actgtgcact gatggcgtgc ttgacgaccg cggggtcgag tccgggagcc
     3481 ccggacttga tgtacatgtc tccgatgtac tcgttcatcg cactcagagc agcgtccatc
     3541 ttgaacgttc cctgtatccc catcatcgag aaattgcgca tgatcgtgta cgtttgcacc
     3601 ttctgagggt gttgtgcctg gattccggct tcgaatcccg gagggatcac gaggacggca
     3661 ctccccttct ctgaagcgac cttgcggaca atgtcatcga cgacaccctc agtgaccgtc
     3721 gtcacctcga agttcgcctt cgtgagaatg cccgaaagca gctcggaggt ctgagtgtgg
     3781 tcgaggtcca cgaccgtgat gggttgtggg gcggcctgct tcttctgctc ctggttaatg
     3841 gtcctgccaa ggaagctgta aatcaccatg acgagcagga taggaatgag cgtctgccac
     3901 gtcagcatct ctcttagttc cttcttgagt acgacgaaga atctcctcat tgcttcacca
     3961 cctgaacgaa gacctcttcc aggttcgcct tgccatactt gtccttaagt ccctggggag
     4021 tcccgatgtc gtggatcgtg cctttgtcga caatcgccac gcggtcagac aggtactcta
     4081 tctcgagcat gttgtgactg gacaggagca cggccatgcc ttcggccgtc gtcctgcgga
     4141 tcgactcacg aatgtcgatc gcggcgacca cgtcgagacc gcttgtcggc tcgtcgatga
     4201 tggccagttt cggatgcgtc atcacagcac gtgacaggag cagctttcgc gtcatgccct
     4261 tgctgtacgt gccgatcttg tcgcgcagcc gttcgcccag tcccgtgata tccgacgctc
     4321 tctggacata ctcgcgctgc gtctcgacgt ccgaagcaaa cagctctgcc atgaagttca
     4381 ggtatccaag acccgtcagg ttcttgtatg ccccggcttc ctcgggcagg tagctgatga
     4441 cctcgcgtat ctgagccgca tccttctgga catccattcc atagacgcgg gcggtgcccg
     4501 acgtcggctg caggatagtc gccacgatac gcagcgtcgt cgtcttgccg gcgccgttgg
     4561 gcccgatgag cgcaaagatc tctccaggag caacctcaaa gcttacgccg
//
LOCUS       DATMZD010000139         2054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_43927,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000139 DATMZD010000000
VERSION     DATMZD010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43927"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1441
                     /gene="cysS"
                     /locus_tag="VN478_02930"
     CDS             <1..1441
                     /gene="cysS"
                     /locus_tag="VN478_02930"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HWQ21663.1"
                     /translation="VPREPGRIAMYVCGVTPYAPAHIGHARTAVVYDLLKRYLRQAGY
                     VVTHVSNYTDVDDKIIRRAQEQGVAPLDLSARYIGEYLEDMASLNVLPPDVTPRVSES
                     IQDIVDLIGRILANGYAYVSSDGVYFSVRKFSRYGRLSGRSVDEMRSGARVDVNEAKE
                     DPLDFALWKFAKSGEISWDSPWGKGRPGWHIECSAMSLRHLGAGFDIHGGGEDLIFPH
                     HENEIAQSEAALEGQQFVRYWMHSGMVNMGAEKMSKSTGNVMTVHELLEQYPGQVIRL
                     YLLQTSYEKPLRFSIAGFGQAFTNYERMVEFEAFLQGFGGTSSADGDMEFTKLCSSFV
                     SELDSSLADNLNTSRALASVYEFMTRVRYSASERTLGGSDIARALEAFRSVLWVLGLD
                     QTGEYVRHKSVLLAEREDTLSVVAAEVGAAVDGGGSASSLLDAIVAKRRAAREAGQYD
                     VADQIRERLRGIGIALEDGVDRVRYRIRG"
     gene            1467..>2054
                     /locus_tag="VN478_02935"
     CDS             1467..>2054
                     /locus_tag="VN478_02935"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HWQ21664.1"
                     /translation="MLIYGKNSVLGALTAEHPVQKIIFEAGSDKDERIRVIKDVASRQ
                     KVPMEYKVGSWFQKSLGDVPHQGIAAQCARLHLLDMNALMGAMPAEPARRSVVLVDQV
                     TDPHNLGAIIRVVAASRAGGLIVTKDRTSPFSPAAVSASAGTAFGLPIAVVANLAYAM
                     ERLKKDGYWLYGLDAADGHDYRKEKYASPAAFIVGS"
BASE COUNT          413 a          543 c          650 g          448 t
ORIGIN      
        1 cgtaccccgt gagccaggaa ggatagcgat gtatgtctgt ggggttacgc cgtatgctcc
       61 tgcccacatc gggcatgctc ggacggccgt cgtctacgac ctcctgaaac gctacctccg
      121 gcaggcgggg tatgttgtga ctcacgtcag caattacact gacgtcgatg acaagatcat
      181 ccggcgcgcc caggaacagg gggtcgcccc gttggacctc tccgctcgct acatcggcga
      241 atacctggaa gacatggcct ctctcaatgt tcttccccct gacgtgacgc cgagggtttc
      301 tgagagtatc caggacattg ttgacctcat tgggcgcatc ctcgcaaatg gctatgccta
      361 tgtctcgtca gatggagtct acttctccgt tcgcaagttc agccgttatg gacgcctctc
      421 tgggcgctcc gtcgacgaga tgcgcagtgg agctcgggtg gatgtgaacg aagcgaaaga
      481 ggatcctctg gatttcgctc tctggaagtt tgcgaagtcg ggtgagatct cctgggacag
      541 cccttgggga aagggtcgcc cgggatggca tatcgagtgc tcggcaatga gtctgaggca
      601 cctcggtgca gggtttgaca tccacggcgg aggggaggac ctcatcttcc cgcaccatga
      661 gaacgagatt gcccagagcg aagcggccct ggaaggacag cagtttgtcc ggtactggat
      721 gcactccgga atggtgaaca tgggcgccga gaagatgtcg aagtccaccg gcaacgtcat
      781 gactgtgcat gaactgctgg aacagtatcc cggtcaggtc atccgtctat atcttctgca
      841 gacctcatat gaaaagccgc tgagattcag tattgccggg ttcggacaag cattcacgaa
      901 ctatgagcgc atggtggagt tcgaggcgtt tctgcaagga ttcgggggca cgtcaagtgc
      961 cgacggcgac atggagttca cgaagctatg ctcgagtttc gttagcgagc tggactcttc
     1021 gctggcagac aacctcaaca catcccgtgc gctcgcttca gtctacgagt tcatgacgcg
     1081 ggtgcgctac agtgcttcag agcggactct cggtggatcg gacattgccc gcgcactcga
     1141 agccttccga tccgtgctct gggtgctggg gcttgaccag acgggtgaat acgtgaggca
     1201 caagtcggtc cttcttgccg agagagagga tactctctca gtcgtggcag cagaggtcgg
     1261 ggccgcagtg gacggcggcg gctctgcgag cagccttctt gatgccattg tggccaagcg
     1321 tcgggccgct cgcgaggcag gacagtacga cgtggctgac cagatcaggg agcgcctcag
     1381 gggtattgga attgccctgg aggacggcgt tgacagagtg aggtatcgca tccgtggcta
     1441 ataatgatag aacaagacca gaaggtatgc taatctatgg taagaactcg gttcttggtg
     1501 cgttgacggc tgagcatcca gtccagaaga tcatctttga agcgggttct gataaggacg
     1561 agcgcatcag ggtgatcaag gatgtggctt cacggcagaa ggtgccgatg gagtacaagg
     1621 tcgggtcgtg gttccagaag agtctcggcg acgttccgca tcagggcatt gcggctcagt
     1681 gcgcacggct acacttgctg gacatgaacg ctctcatggg tgcgatgccg gctgagcctg
     1741 cacgccgttc ggttgtgctc gtggatcagg tcacagatcc tcacaacctt ggagcgatca
     1801 ttcgtgtggt tgctgcttca agagctggtg gtctgatcgt cacgaaggac cggacgagcc
     1861 ccttctcgcc tgctgccgtg tctgcctcgg ctggaaccgc gtttggtctt ccaattgccg
     1921 ttgtggccaa cctggcgtat gccatggaac ggctcaagaa ggatggctac tggctgtacg
     1981 gactggacgc tgcggatggc catgactacc gcaaggagaa gtatgcatct cctgctgcct
     2041 tcattgtcgg gagc
//
LOCUS       DATMZD010000140         2317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_104675,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000140 DATMZD010000000
VERSION     DATMZD010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104675"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..>2317
                     /locus_tag="VN478_02940"
     CDS             111..>2317
                     /locus_tag="VN478_02940"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HWQ21665.1"
                     /translation="MKRIENTERRRAGAMPRSLEQAAFWYVAVLVTAGTFLGAGMISL
                     WSQLTGIALCIPAVAIVVWRATRSDEAWAAVIADRMNLLLALGVALLVVSTTVSVYRS
                     SSLVELCKGIGLVGLFSFSREYLSSAPRRRDVAHLLYWTGVGVSLAGIVLFGVRGSSA
                     VSISRLTHMLLVVDSERLSIGFGYANSFAAFLLVPLAAGLGLVLDAASMRRRAVLVAG
                     SLIMLVAMLESSSRGGLIVLGCMVLAGSPLIVRASGGGRNAWKRLGFFYGALGAVLAV
                     LALIPPLRQAVLDPIVKRLLSIGTELVTGNAILSSTRGRLTMIGDALRYAHAYPILGS
                     GAGTYGSVYMRFRTTMFFASDPHSLPLKILAETGVVGLGLGLAMLIAVFRRFWHSVND
                     AGRVGYLGVALWLGSAGLLLHACIDWDFAFLAFPIVLSVVCGIYVQWPPKRADAPAGV
                     MSAEASRHRDGYMAAGHARRGLGLVAPGLRRMARPVVACWVVTCVMLLAGAILASAAV
                     HLSGVHPDRALALLNTASSIDPLNAEYPYRSAGINVALDAQAPGGPESDRALAIRGDF
                     ERAMVLDRYFPLYRIDYGRFLLRYGLSDAVQTYEDLVKIDPMDPGTYTGLAASYLIVY
                     ENVDKAEYWVGKALTIDAAYAEAHLVKGMVFEKRGDVAKAATEYQKTSELDTTGTDAL
                     LRLAAMEREHGDLRAASRALSEANVRAPRDASVRSALAAIAPVVDVSEPQADY"
BASE COUNT          390 a          668 c          732 g          527 t
ORIGIN      
        1 ttgcatactc tcaagatgtt cttcatgccc gaacgacgga actagtcgag ttgatggact
       61 gtcacaggtc tggcgtttga ctatccacgg cccgagcggt atcatgaatc gtgaaaagaa
      121 tcgaaaacac tgagcgccga cgcgctggtg ctatgccccg gagcctcgaa caagctgcgt
      181 tctggtacgt cgcagttctg gtgacagccg gcaccttcct gggtgccggg atgatctcgc
      241 tctggtcaca gctcaccgga atcgcgctct gcatcccggc cgttgcgatt gttgtgtgga
      301 gagccactcg ctcggacgaa gcctgggcag ccgtcattgc tgaccggatg aatctcctgc
      361 tcgcgctggg agtggcgttg ctggttgtct caaccactgt gtctgtctac cgttcgtcgt
      421 cgctcgtaga actgtgcaag gggattgggt tagtcggact gttctcgttc tcacgggagt
      481 acctttccag cgcgccgcgc cggcgagacg ttgcgcacct cctctattgg actggtgtcg
      541 gggtctcctt ggcgggcatt gtgctgttcg gtgtcagggg ttcctccgcc gtcagcataa
      601 gtcgcctgac gcatatgctt ctggtcgtgg acagcgagag gctctccatc ggtttcggat
      661 acgcgaacag cttcgctgcg ttcctgctgg tgccattggc cgccggactg ggtttggtgc
      721 tggacgctgc atccatgagg aggcgagcag tgctcgtcgc tggatcgctg atcatgctgg
      781 ttgctatgct ggaatccagc tcgcgcggcg gactcatcgt cctggggtgc atggtcctcg
      841 ccggttctcc gctcatcgtc agagcctccg gcggtggaag gaacgcctgg aagcggctgg
      901 gcttcttcta tggagccctc ggtgccgtcc tggctgtgct ggccctcatc cctccgcttc
      961 gtcaggcagt tctggatccc atagtgaagc gacttctcag cattgggacg gaactagtga
     1021 cggggaacgc tattctctcc agcacgcggg gtcggttgac catgattggc gacgcccttc
     1081 ggtatgcgca tgcatacccg atactcggca gtggcgcagg cacatatggc agtgtctata
     1141 tgcggttcag gacaacgatg ttcttcgcaa gcgaccccca ttctcttccg ctcaagatac
     1201 tggccgagac aggcgttgtc ggtctggggc taggtctggc gatgctaatt gcagtattcc
     1261 gccggttctg gcacagcgtg aatgatgccg gacgtgtggg gtacctggga gtggctttgt
     1321 ggctggggag tgcaggtttg ctgctgcacg catgcatcga ctgggacttc gcctttctcg
     1381 cctttccgat tgtgctctcg gtcgtttgtg gcatatacgt tcaatggccg ccaaagcggg
     1441 cggacgcacc tgcaggggtg atgtctgctg aagcgtccag gcaccgggat ggctatatgg
     1501 cggctgggca tgccagacgc gggcttggcc tcgttgcccc gggcctgagg cgcatggctc
     1561 gccccgtcgt ggcgtgctgg gtggtgacat gcgtcatgct tctagcgggg gccatccttg
     1621 catcagccgc cgtacacctt tccggtgtgc atcccgacag agcgctggct ctcctgaata
     1681 cggcctcatc catcgatccg ctgaatgctg agtaccctta ccgcagtgcc ggcatcaatg
     1741 ttgccctcga cgcccaggcg cctggtggac ctgaatcgga cagggccctg gctatccgag
     1801 gcgatttcga gcgggccatg gtcctggacc gttacttccc tctgtaccgc atcgactatg
     1861 ggcgttttct gctcagatat gggttgtcag atgccgtcca gacctatgag gacctggtga
     1921 agatcgatcc catggaccca ggaacatata cgggtcttgc agcgtcgtac cttatcgtct
     1981 acgagaacgt cgacaaggct gagtactggg tcgggaaggc cctgacaatt gatgcagcat
     2041 acgccgaggc gcacctcgtg aaggggatgg tcttcgagaa gcgcggcgac gttgccaaag
     2101 ccgccacgga atatcagaag acctccgagt tggacactac tggcacggat gcgttgctca
     2161 ggcttgcagc aatggagaga gagcacgggg atctcagagc tgcatcccgc gccctcagcg
     2221 aggcaaacgt gcgggccccc cgtgacgcgt cagttcggtc ggcactggca gcgatagccc
     2281 ccgtagtcga cgtttcagaa cctcaggcgg actacca
//
LOCUS       DATMZD010000141         6706 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_124560,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000141 DATMZD010000000
VERSION     DATMZD010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6706
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124560"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="VN478_02945"
     CDS             complement(<1..706)
                     /locus_tag="VN478_02945"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HWQ21666.1"
                     /translation="MEDNRIATHPILAWNRGDPVHVTYDGKEIAAYPQETVAMALYAA
                     GVKQYSTSSRFYRPRGMFCAIGKCSSCMMRVDGIPNTRTCVLQVRDGMEVETQHGDGK
                     FPDVQAAMKGVKEKDAEVLVIGGGPAGLEASLWAAKSGADVILLDQNPQLGGQLVKQT
                     HKFFGSAKENAGTRGITIAKKLIEEVKAAPNIDVYSGFTAFGWFQGSICAADQDHAVL
                     FHPRKVIIATGAAEKML"
     gene            1061..2227
                     /locus_tag="VN478_02950"
     CDS             1061..2227
                     /locus_tag="VN478_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HWQ21667.1"
                     /translation="MRKTVSVVLVCMLAAVLFLSGCAKKTGDDAGPIQIALQAPITGD
                     YAYEGQMAKQSVEVAAELINKAGGVLNRQIEVTIVDDGSNPKDSAIAAQKAVSLKVVA
                     VIGSYGSSVTEPAIDIYEKNKMVAVGYGCTAIRLTMDKQRTYFFRTCGRDDAQGLFFA
                     KYAVETMGAKRIAIMHDNSTFAKGVAEQAELALKPYVDAGKCTIVYNDAITPGEKDFS
                     SAVTKLRNTTPDIWYYTGYYTEAGLLIRQARDAGLTCPFVGGNAAINDDFIKIAGIAV
                     AKGALMTQEPLVSDVTTTIAEQFRTLYKEKFGDVPSSPWPVYAADGLYAIVGAIKNAN
                     STEPDAIATAMHTNMTGVEGVTGPILFSDRGDRQDVPYKMYEVTADGKYVVRAQ"
     gene            2257..3168
                     /locus_tag="VN478_02955"
     CDS             2257..3168
                     /locus_tag="VN478_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015946377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWQ21668.1"
                     /translation="MATFFEQLINGLTIGSFYALVALGYSMVYGVMKLINFAHGDLFA
                     LGSYLGYTLLVAGSSWVTVHFGLWGGMAVAMMFAFIGIGLAGVLMERVAYRPVYPAGR
                     LSLVVSALGMSIFLQNGIMALWGSRPQAYPAAVVPSTRFSLLGLPVTTLQVVILLLSF
                     TLMLVIWFIVEKTTFGAAIRATALDRSTATLMGIDVRVVIFFIFALGPALGGIAGVMN
                     GMYYRAISFNMGWTYGLKAFTATIMGGIGNIPGAMIGGLLLGLMESLFAGYVSGAWKD
                     VFVFVILIVVLLWRPTGLLGEKTAEKV"
     gene            3179..5095
                     /locus_tag="VN478_02960"
     CDS             3179..5095
                     /locus_tag="VN478_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HWQ21669.1"
                     /translation="MGITESILKRVGLGKPTGDQPATFWSKNRGWILLAVTGALFAAY
                     PFLPFISDYWIDVAFFVGIYALLGLSLNIVLGEVGLFDLGHTAFYAIGAYVTAILNTT
                     LHVPIVILLPLSAVIAGGFAYLVTSPIIHLKGDYLCIVTIGIGEIVRLTMINNPFNLT
                     GGPNGINGIGGPNLFGLAINSSTQFYFYTWVIIGVVVFGLVRLQHSRIGRAWNYIRED
                     ETAAEASGVDVRSYKLLAFVLGAALAGVAGNIYASKQMSVSPQSFTFIESSLLFCIVL
                     LGGLGSIPGSILGAAVVVVFPEIFRQFASYRLLFFGLALMLMMIFRPGGIWPRKREGA
                     GLDGLGIRGLPKNDDDLIPLGKPDPAILKPATTAVVRGVRLPPVTVAMDEEMLLQTHG
                     ATLKFGGLTAVNNFDLSVRRGKITSLIGPNGAGKTTLFNMITGIYKPTAGQIDFLGRD
                     ITGLKPHTIISYGIARTFQNIRLFPSLTCIENIMSGPHCHARSGTWAAVMHTIAEVRE
                     ERDIVATAAYRLDQVGLWKYRNELAKNLPYGKQRYLEIARALATAPHLLILDEPSSGL
                     NDKESEDLMVLLQSLVKEGFTLLLIEHDMQVVMGISDWVAVMDMGQKIAEGLPKDVYN
                     NQRVIEAYLGKEDD"
     gene            5097..5837
                     /locus_tag="VN478_02965"
     CDS             5097..5837
                     /locus_tag="VN478_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWQ21670.1"
                     /translation="MATLLAVKNLVVNYGAVQALKGIDVELGEHDIVAVLGANGAGKT
                     TLLKSISRIQTIKAGTIAYKGKDLATVPAFHLAGLGIAHVPEGRHVFATLTTEENLNL
                     GAYGVRSMPAGREMEKTKEWIFGLFPILKERRKQLAGTLSGGEQQMLAIGRGLISKPH
                     VLLLDEPSLGLAPIIVQEIFRVIRQIHEEEGVSILLVEQNARKALAVARYGYILELGK
                     VAIEGDACDLKNDERVRSAYLGGSKVNC"
     gene            6011..>6706
                     /locus_tag="VN478_02970"
     CDS             6011..>6706
                     /locus_tag="VN478_02970"
                     /inference="COORDINATES: protein motif:HMM:NF014750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HWQ21671.1"
                     /translation="MKTIEEMRQAHKVLATYSFTPGGVDKGYTNRRLYVNLTAKIIEE
                     RPIEQMVKDKFTGGRGYGLYYLWQAVKPTTRWNDADNELIFTAGPLTGLTQYPGSGKT
                     HSVTLSPETGLPVDNNGGGFFAPFMKFSGFDCVEIQGKATEDVMLVIDGNKGIVTIET
                     APEEPTNSYDLPEVLHAMYADGDDDRKNVSVATSGEGAEHSLLGLINLSYWDAKRKRT
                     RIKQLARGGPGTVF"
BASE COUNT         1379 a         2086 c         1898 g         1343 t
ORIGIN      
        1 tcagcatctt ctcggccgcg cctgtcgcga tgatgacttt gcgcgggtga aacagcactg
       61 catgatcctg gtcggcagca cagatggatc cctggaacca tccaaatgca gtgaagccac
      121 tgtagacgtc gatgtttggc gcagctttga cttcctcgat cagcttcttt gcaatcgtaa
      181 tgccacgcgt cccggcattc tccttcgccg acccgaagaa cttgtgggtc tgcttgacca
      241 gctgaccacc caattgggga ttctggtcca gcaggatgac gtccgcaccg gacttggccg
      301 cccagagaga tgcttccaga cctgccggcc cgccgccgat gaccaggact tcagcatcct
      361 tctccttgac acccttcatg gccgcttgca cgtcggggaa cttgccgtcg ccgtgctgcg
      421 tctcgacttc catcccatcg cggacctgca ggacgcaggt gcgcgtattc gggatgccgt
      481 cgacacgcat catgcaggac gagcacttgc cgatggcaca gaacatgccc cggggacggt
      541 agaaccggga ggaggtgctg tactgcttca cgccggctgc gtatagagcc attgccactg
      601 tttcctgggg gtaggcggcg atctccttgc cgtcgtacgt gacatgcacc gggtctcccc
      661 ggttccacgc aaggatcgga tgcgtggcaa tacgattgtc ttccactttt ttcacctctc
      721 cgatgcgaat tggtttccta cgtctgcagt atatgagcac cgggagtttt ttctacaagc
      781 ccgtgaaaaa aagcacaact cccggacacg agatcgtgcc tcaggtgtca tcaagacaag
      841 atctgctggc atgcggacct tgtcgcccgg tccagccgac agccgaattg accttgtatt
      901 tttcccgaca tctatcaaca tggtgctgaa ccacagtctc aggaggcaca agagaaaaag
      961 cggacttcga caagacggta cggcaccaca ggctctaccg aaaaagctcg tgctgggaac
     1021 taatctcacc acattcgcgc gagaaatagg aggtaggaga atgagaaaga ccgtatcagt
     1081 tgtgctcgtt tgcatgcttg ccgcagtgct cttcctttcc gggtgtgcca agaagaccgg
     1141 cgacgatgcc ggacccatcc agatagcgct gcaggctccc atcactggtg actacgctta
     1201 tgaaggtcaa atggccaaac agtcggtcga agtcgccgcg gagctcatca ataaggccgg
     1261 aggtgtcctc aatcgccaaa tcgaggtcac tatcgtcgac gatggctcca accccaaaga
     1321 cagtgccatc gccgcccaga aggcagtctc cctcaaggtc gtcgccgtca tcggcagcta
     1381 tggttcgtcg gtcaccgagc cggctatcga catctatgag aagaacaaga tggtcgccgt
     1441 cggatatggc tgcaccgcta tccgtctgac catggacaag cagcgcacgt acttcttccg
     1501 cacgtgtggc cgtgatgatg ctcagggcct cttctttgcg aagtatgccg tcgagacgat
     1561 gggcgccaag cgcatcgcca tcatgcacga caacagcacc ttcgccaagg gcgttgcaga
     1621 gcaggctgag ctcgctctca aaccgtacgt agatgccggc aagtgcacga tcgtctacaa
     1681 cgacgccatc acacccggcg agaaagactt ctcctctgct gtcacaaaac tccgcaacac
     1741 cactcctgac atctggtact ataccggcta ctacaccgag gcaggtctgc tcatccggca
     1801 ggcacgcgac gccggtctga catgtccgtt tgttggtgga aacgcagcca tcaacgacga
     1861 cttcatcaag atcgcgggca tcgccgtggc taaaggagcc ctcatgacgc aggaacccct
     1921 ggtaagcgat gtgacgacca cgatcgccga gcaattccgc acgctgtaca aggagaaatt
     1981 cggcgacgtg ccctccagcc cgtggccggt ctacgctgct gacggtctct atgctatcgt
     2041 cggagcgatc aagaacgcca actccaccga gcccgatgcc atcgccaccg ccatgcacac
     2101 aaacatgact ggtgtcgaag gcgtcaccgg ccccatcctg ttctccgaca ggggcgaccg
     2161 ccaggacgtc ccctacaaga tgtatgaggt gaccgctgac ggcaagtacg ttgttcgcgc
     2221 acaatagtca taacccctga ttgaagcgag gtcaccatgg ccacgttctt tgaacaactg
     2281 ataaatggcc tgaccattgg ctcgttctac gcgctggtgg ctctgggcta ctcgatggtg
     2341 tacggtgtca tgaaactgat caacttcgcc catggtgacc tcttcgccct gggctcgtat
     2401 ctgggctaca cccttctcgt tgcaggttcg tcctgggtga cagtccattt cgggctttgg
     2461 ggagggatgg ccgtcgcgat gatgtttgcc ttcatcggaa tcggtctcgc aggcgtcctg
     2521 atggaacggg tcgcgtaccg ccccgtctat ccggcaggca ggctctcgct ggtcgtctcc
     2581 gcactcggca tgtccatctt cctgcagaac ggtatcatgg ccctctgggg ctcccgtcct
     2641 caggcgtatc ccgcagccgt cgtcccctct acgcgcttct ccctcctggg actgccggtg
     2701 accaccctgc aggtcgtcat cctgctgctc tcgttcacgc tcatgctggt catctggttc
     2761 atcgtcgaga agacgacgtt cggagcggcc atacgcgcta cagcactcga ccgcagcact
     2821 gcgacactga tgggtatcga cgtccgtgtc gtcatctttt tcatatttgc cctgggccct
     2881 gcgctcggcg gcatcgccgg cgtcatgaac ggcatgtact accgcgccat cagtttcaat
     2941 atgggatgga cctacggtct taaggccttc acggccacga tcatgggcgg catcggcaac
     3001 atcccgggcg ccatgatcgg tgggttgctc ctgggactca tggagtcact gttcgcgggc
     3061 tacgtcagtg gagcctggaa agacgtgttc gtgttcgtca tcctcatcgt tgtcctcctc
     3121 tggcggccga caggcctgct gggcgagaag acggcggaaa aggtctgagg tcgctgccat
     3181 gggaatcaca gagagtatcc tcaagcgtgt cggtctgggc aagcccacag gcgaccagcc
     3241 tgccacgttc tggtcaaaga accgtgggtg gatcctgctc gccgtcactg gagccctttt
     3301 cgccgcctat ccattcctgc ctttcatctc cgactactgg atcgacgtcg ccttcttcgt
     3361 cggcatctat gcactgctgg gcctcagcct caacatcgta ctgggtgagg tcggcctgtt
     3421 cgatcttggc cacacggcat tctatgctat cggcgcctat gtcactgcga tcctgaacac
     3481 gactctgcac gtgccgattg tcatcctgtt gccgctgagt gcagtgatcg ccggcggctt
     3541 cgcctacctg gtcacctcac ccatcatcca cctgaagggc gactacctgt gcatcgtgac
     3601 catcggcatc ggcgagatcg tccgtctgac gatgatcaac aacccgttca acttgaccgg
     3661 aggacccaac ggcatcaacg gcatcggcgg gccgaacctc ttcggactgg caatcaactc
     3721 atcgacgcag ttctacttct acacttgggt catcatcggg gtcgtcgtct tcggcctcgt
     3781 gcgactccag cattcgcgca tcggcagagc gtggaactac atccgtgagg acgagaccgc
     3841 cgctgaggca agcggcgtcg acgtgcgttc ctacaagctg ctggcttttg tcctgggtgc
     3901 tgcacttgcc ggcgtcgcag gcaatatcta cgcctccaag cagatgtccg tctcgccgca
     3961 gagcttcacg ttcatcgagt cttccctgct cttctgtatc gtcctcctgg gaggcctggg
     4021 gtccatccct ggcagtatcc tcggggcggc agtggtcgtc gtgttccctg agatattccg
     4081 gcagtttgcc agctaccgac tgctgttttt cggacttgcg ctgatgctca tgatgatctt
     4141 ccgtccggga ggcatctggc cacgcaagag agagggcgcc gggctcgacg gcttaggcat
     4201 ccggggtctt cccaagaacg acgatgacct catccctctt ggcaagcccg accccgccat
     4261 cctcaagccg gccacgacgg ccgtagtcag gggagtcagg ctccctcctg tgactgttgc
     4321 aatggatgag gaaatgctgc tgcagacaca cggcgccacg ctgaagttcg gcgggttgac
     4381 cgccgtcaac aacttcgacc tcagcgtccg gaggggcaag atcacgtcgc tcatcggacc
     4441 gaacggtgcg ggaaagacga cgctgttcaa catgatcaca ggcatctaca agcctaccgc
     4501 cgggcagatc gacttcctgg gacgcgacat tacgggtctc aagccccaca ccatcatcag
     4561 ctacggcatc gcccggacct tccagaacat ccggctgttc ccgtcgctga cgtgtattga
     4621 gaatatcatg tccggtcccc attgccatgc gcggtctgga acatgggccg ccgtgatgca
     4681 tacaatcgcc gaagtccgcg aggaacgcga catcgtcgcg accgctgcct accgtctcga
     4741 tcaggtcggc ctgtggaaat accgcaacga acttgccaag aatctgccgt acggcaagca
     4801 gcgctacctc gagatcgccc gcgcgctcgc gacagctccc catcttctga ttctggacga
     4861 accctcctca gggctgaacg acaaggagag cgaggacctg atggtcctgc tgcagtccct
     4921 cgtcaaggaa gggttcacgc tgttgctcat cgaacacgac atgcaggtcg tcatgggaat
     4981 ctccgactgg gtcgccgtca tggacatggg tcagaagatc gcagaaggcc tgccaaagga
     5041 cgtctacaac aaccagcgcg tcatcgaagc ctatctgggc aaggaggatg actgacatgg
     5101 cgacactcct ggctgtcaag aacctcgttg tcaactacgg ggcagtacag gccctgaaag
     5161 ggatcgacgt cgagctgggc gagcacgata tcgtcgcggt gctcggcgca aacggggcgg
     5221 gcaagacaac actgctcaag tccatctcac gcatccagac gatcaaggcc ggcaccatag
     5281 cgtacaaagg aaaggatctc gccacggtgc ccgcttttca tctcgcagga cttggcattg
     5341 cccacgtgcc tgaaggcaga cacgtcttcg cgacccttac gacggaggag aacctgaacc
     5401 tgggcgccta cggcgtccgg agcatgcctg ccggcaggga aatggagaag accaaggaat
     5461 ggatcttcgg cctcttcccg atcctcaagg agcggcgcaa gcagctcgca gggaccctct
     5521 cgggcggcga gcagcagatg ctggccatag ggcgcgggct catctccaag ccgcatgtcc
     5581 tgctactgga cgaaccctca ctggggcttg ctcccattat cgtccaggag atcttccggg
     5641 tcatccggca gatccacgag gaagagggcg tctccatcct gctggtcgaa cagaatgccc
     5701 gcaaggcgct ggcggtagca cgttatggct acatcctcga actcggcaag gtcgccatcg
     5761 agggtgacgc gtgcgacctc aagaacgacg agcgcgtccg ttccgcctat cttggaggtt
     5821 ccaaagtcaa ctgctgacct gaatcagtgt tcctccactc tggggcccgg cacaagccgg
     5881 gccctcctgt cgaaagagag gaatcgccga cgcagacggc cgatacgagc acacgaacga
     5941 gcgctgcctt gaacgacgcg tcgattgaaa taccatgtga acggattcac gatgaaccgg
     6001 gaggtagaaa gtgaagacca tcgaggaaat gcggcaggca cacaaggtcc tggcaacgta
     6061 ctccttcacg ccaggcggcg tggacaaggg atataccaac aggagactgt acgtcaatct
     6121 tactgcgaag atcatcgagg aacgccccat cgagcaaatg gtcaaggaca agttcactgg
     6181 aggccgtggt tacggcctgt actacctgtg gcaggccgtc aagccgacca cgcgctggaa
     6241 tgacgccgac aatgagttga tcttcactgc ggggcctctc acgggactca cgcagtatcc
     6301 gggttcgggc aagacacact cggtcactct ttcgccggag acgggtctgc ccgtcgacaa
     6361 caacggcggc ggcttcttcg ccccgttcat gaagttctcc ggcttcgact gcgtcgagat
     6421 ccagggcaag gcaactgaag acgtcatgct cgtcattgat gggaacaagg ggatcgtcac
     6481 catcgagact gcgcccgagg agccaaccaa cagctacgat ctgcccgagg tgctgcatgc
     6541 catgtatgcc gacggggacg atgaccgcaa gaacgtcagc gtggccacgt ccggcgaggg
     6601 tgcagaacac tcccttctag gcctgatcaa cctcagctac tgggacgcga agcgcaagcg
     6661 aacgcgcatc aagcagctcg cgcgtggcgg gcccggcacc gtcttc
//
LOCUS       DATMZD010000142         4616 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_14313,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000142 DATMZD010000000
VERSION     DATMZD010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4616
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14313"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="VN478_02975"
     CDS             <1..567
                     /locus_tag="VN478_02975"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HWQ21672.1"
                     /translation="KRNMFWLIVAAIIPSGVVGVLFSSTIEKAFASLYVVAAMFLVTA
                     ALLFLQRFSSTSRTIREAGWKDALTIGVGQVFALLPGLSRSGTTMSFGVYRGFKQEDA
                     AHFSFLMAIPTIAGAAVFKIKDLLQAGLSAGALTELGVGVAVSVLTSLVAIGLLLKIA
                     RSGKMHWFALYCVGMAVFTFAAHYFGLI"
     gene            641..2344
                     /locus_tag="VN478_02980"
     CDS             641..2344
                     /locus_tag="VN478_02980"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HWQ21673.1"
                     /translation="MFEKGIGKRDIGELALSLGIRDEEVDFFGKYMAKIDYHILNRLQ
                     DKPDGKYIDVTAITPTPFGEGKTVTTIGLGMAMNRLGFKTVNALREPSMGPVFGIKGG
                     GTGGGKSSLFPMEQINLHFTGDIHAVETATNLLAAVVDNHISQGNELDIDPQSISWRR
                     TMDVEDRSMRKLTIQLTEKRDGIKTDTSYETGFDIAAASPIMAIMGLTAGFEDLHQRL
                     SEIVVARSRSGKSVTVADLRATGGLAAVLKDAIYPNLVQTEEGTPSFVHIGPFANIAF
                     GNNSVLSDLIALKLGDYVVTESGFGADMGFEKLMDIKLRQAGMKAPDCVVIVATIRAL
                     KMHGGAFTIKPGKKLDPALVSAVNMPALDLGCENLRVHIENVRSYGLPVVVAVNRFPD
                     DTAEEIAMVQAKALQFGAVAAVPHTFFVDGSEGGLELARAVQEVASTSRVGTVHYPYE
                     LTDTIEEKMRKLVRSIYRAGDIDLTPVAVERIKEFTAAGYDRWPICMAKTHLSIGHDP
                     DVKGAPSGYLFPIRDIRAATGARFLYPLGGTMQTMPALSKVPAMVNIDVTGDGVITGL
                     R"
     gene            2445..3023
                     /locus_tag="VN478_02985"
     CDS             2445..3023
                     /locus_tag="VN478_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HWQ21674.1"
                     /translation="MKQTFLNLGAAFVGESMARNRYTMYNKVARTEGFEQIAGIFAET
                     ADQERVHARVLFELLQDIKAENGGDLKLANAEVPLVLGTTAENLQAAIDGEHYETTTM
                     YPGFADVADQEGYKAIAARLRAIAVAEAHHEERYTKLLKEVKAQSVFKKDHKIVWICR
                     ECGYVYEGTEPPAKCPACQHPSSFYQVKDEDY"
     gene            3274..3945
                     /locus_tag="VN478_02990"
     CDS             3274..3945
                     /locus_tag="VN478_02990"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HWQ21675.1"
                     /translation="MSANKKKATQRVSQVQELQKKYPGHRPIAPSKRTVVTTLVATVV
                     LSLLMVFAFYKKAQAPQLVGTKYVTLETTMGTIKLELDGDKAPRTVTQFLKNVNAGAY
                     DGLSFHRVVKGFMIQGGDPNGNGTGGTDVPFEKTDISHVRGVISMASSQQGVTQSDMQ
                     FFIMHGDYTGLDGAFAAFGHVVDGMDVVDKIASVAVGPNSSVPPEVSVPLTRITIIRA
                     TEVAN"
     gene            3955..4440
                     /locus_tag="VN478_02995"
     CDS             3955..4440
                     /locus_tag="VN478_02995"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004837133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HWQ21676.1"
                     /translation="MEEKNRFVSMETSKGTMKIELYAARAPKTVERFMVAVNAGKYDG
                     LKFHRIIKDFMVQGGDPQGNGMGGGNVQYEGSDVKHVPGVISMAAQQAHVDQSDMQFF
                     IMTSTSSQLDGNYTAFGHVTEGMDVLAAIAATPVAQAPWGERSRPVEDVRIVKASEIP
                     E"
     gene            4459..>4616
                     /locus_tag="VN478_03000"
     CDS             4459..>4616
                     /locus_tag="VN478_03000"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HWQ21677.1"
                     /translation="MAIHIVTDSTSCLPTGFAEQHGLPVVLLKVSVDGVFFREFSEIA
                     PDVFYAKQ"
BASE COUNT          998 a         1326 c         1401 g          891 t
ORIGIN      
        1 aagcgcaaca tgttctggct catcgttgca gccatcatcc cgtccggcgt cgtcggagtc
       61 ctgttcagtt cgaccatcga gaaggctttc gccagtctgt acgtcgtcgc ggccatgttt
      121 cttgtcacgg ccgccctgct cttcctgcag cgcttttctt cgaccagcag gacgattcgc
      181 gaagccggct ggaaggacgc gctgaccatt ggcgtcggtc aggtgtttgc actgctgcca
      241 ggcctctcgc gaagcgggac gacgatgtct ttcggcgtct acaggggctt caagcaggag
      301 gatgcggccc atttcagctt cctcatggcc atcccgacca ttgccggtgc tgcggtgttc
      361 aagatcaagg acctgctgca ggctgggctt tccgcaggcg cccttaccga gctgggtgtc
      421 ggcgtcgccg tcagcgtact taccagcctt gtcgcgatcg ggctgctgct caagatcgcg
      481 cgaagcggca agatgcactg gttcgcactc tactgcgtcg gcatggcagt cttcaccttc
      541 gcagctcact atttcggact gatctgagcc tctccggctg tgcgggaatc ggattgaggg
      601 tacaatgaca ggaaggacca cctggaaggg agtgtgaccc atgttcgaaa aaggaattgg
      661 caagagagac attggcgagc tcgcgctgtc gctgggtatt cgtgacgagg aagtcgactt
      721 ctttggcaag tacatggcca agatcgacta ccatatcctc aaccgcctgc aggacaagcc
      781 ggatgggaag tacatcgacg tgacggctat cacgcccacg ccgtttggtg aaggaaagac
      841 tgtcacaacg atcgggctgg gtatggccat gaacagactt ggcttcaaga cggtgaacgc
      901 tctgcgcgaa ccgtccatgg gaccggtctt tggcatcaag ggtggaggca ccggcggcgg
      961 caagtcttca ctcttcccga tggagcagat caacctgcac ttcacggggg acatccatgc
     1021 ggtggagacg gcaaccaacc tgctggccgc cgtcgtggac aaccacatct cgcaggggaa
     1081 cgaactggac atcgatcctc agagtatcag ctggcgcagg acaatggacg tcgaagaccg
     1141 gtcgatgcgc aagctgacga tccagctgac agagaagagg gatggcatca aaacagacac
     1201 gtcctacgaa acaggattcg acattgccgc cgcttccccg atcatggcca tcatgggcct
     1261 cacggcggga ttcgaggacc ttcatcagcg tctgtcagag atcgtcgtcg cgcgcagccg
     1321 cagcggcaag tccgtgacag ttgcagacct ccgggcgacc gggggtttgg ccgccgtgct
     1381 caaggacgcc atctatccca acctggtcca gaccgaagaa ggcacgcctt ccttcgtcca
     1441 catcgggccg ttcgcaaaca ttgcctttgg caacaactcc gtgctgtccg acctcatcgc
     1501 cttgaagctt ggcgactacg ttgtgacaga gagtgggttt ggtgcagata tgggcttcga
     1561 gaagctcatg gacatcaagc ttcgacaggc cggtatgaag gcccctgact gtgtggtcat
     1621 cgtcgctacg atccgcgcgc tcaagatgca cggtggcgca ttcaccatca agccaggcaa
     1681 gaagctcgat ccagccctcg tttcagcggt caacatgcct gcactcgacc tgggctgcga
     1741 gaacctccgg gtgcatatcg agaacgtccg ttcctatggt ctgcccgtgg tcgtcgccgt
     1801 caacaggttc ccggacgaca cggccgagga gatagccatg gtccaagcca aagccctgca
     1861 gttcggtgcc gtggcggccg tccctcatac gttcttcgtg gatggcagtg agggtgggct
     1921 ggagctggca cgtgccgtcc aggaagtagc gtcgacgtcc agagtgggca ccgtccacta
     1981 tccatatgaa ctgaccgata ccattgagga gaagatgcgc aagctggtcc gctcgatcta
     2041 tcgtgccggc gacatcgatc tgactcctgt ggcagtcgag cgtatcaagg agttcactgc
     2101 cgcaggatat gatcggtggc ccatctgcat ggcaaagaca catctgtcca tcggccatga
     2161 ccctgacgtc aagggtgcgc cctctggata cctcttcccg atcagggaca ttcgtgccgc
     2221 gaccggagca cgctttctgt acccgcttgg tggaaccatg caaaccatgc cggcactgtc
     2281 aaaggtccct gccatggtca atatcgatgt gacgggagac ggcgtcatca caggccttcg
     2341 ctagactgtc aagcagaccg gcactgtgaa cattctcctt tgtgccgcag gcggtcatcg
     2401 gatacaatgg gtccagtagc acaaactgcg tatggaggtg agcgttgaaa cagacatttc
     2461 tcaatttggg tgcggctttt gtaggcgagt ccatggctcg caaccggtac accatgtaca
     2521 acaaggtagc ccgaaccgaa ggatttgagc agattgcagg gatcttcgcg gaaaccgccg
     2581 atcaggaacg tgttcacgcc agagtcctgt tcgaactcct gcaggacatc aaggccgaga
     2641 acggtggcga tctgaagctc gcgaacgcag aagtcccgct ggtgctgggg acgacggcgg
     2701 agaatctgca ggctgccatc gacggcgaac actatgagac gaccacgatg tatcccgggt
     2761 ttgcggacgt cgccgatcag gaaggctaca aggcaattgc cgcgcgcctg cgtgccattg
     2821 cggtagccga ggcgcaccac gaggagcggt acaccaagct tctgaaggaa gtcaaggcgc
     2881 agtcggtctt caagaaggat cacaagatcg tctggatctg ccgtgagtgc gggtacgtgt
     2941 atgagggaac cgagccaccg gcgaagtgtc ctgcatgcca gcatccatca agtttctatc
     3001 aggtcaagga cgaggactac tagagtcaga gcgatactat tggggcctgc tgcggcaggc
     3061 ccttctgtgt cagacggcga actactgcgt cgctctgccg gacagtctca caggcccgga
     3121 tgccacgtgg acgtcgcaga tgagatcagc agtgcacctg gccacaggcc tggcgccggc
     3181 tgacgattgt cgtgtgatgc atggggacca ctcaggcaac aacgcctggt gggagcacgg
     3241 tcaaattgga gactgtcagg agtggaaccg ttgatgagtg ccaacaagaa gaaggctaca
     3301 cagagagtga gtcaggtaca ggaattgcag aagaagtatc cggggcacag gcctatcgcg
     3361 ccaagcaagc ggacggtagt cactacgctc gtcgcgacgg tcgtcctgtc gctgctgatg
     3421 gtgttcgcct tctacaagaa ggctcaagct ccgcagctcg tgggcacgaa gtatgtcaca
     3481 ctggagacca ccatgggcac catcaagctt gaactcgacg gggacaaggc ccccaggact
     3541 gtcacccagt tcctcaagaa cgtcaacgcc ggcgcctacg acgggctgtc attccatcgg
     3601 gtcgtcaagg gattcatgat tcagggggga gaccccaacg gcaatggaac gggagggact
     3661 gacgtcccat tcgagaagac ggacatcagc catgtccggg gtgtcatctc catggcatca
     3721 tcacaacagg gagtgactca gtccgacatg cagttcttca ttatgcatgg cgactacacg
     3781 ggtctcgacg gagcgttcgc agcgttcggc catgtcgtgg atggaatgga cgtcgtcgac
     3841 aagatcgcca gcgtggctgt cgggccgaac tccagcgtac ccccggaagt ctcggtccct
     3901 ctcaccagga ttaccatcat ccgggcgact gaagtcgcca actaggagag tcacatggaa
     3961 gaaaagaaca gattcgtgag catggaaacg tccaagggca cgatgaagat cgagctgtac
     4021 gccgcccgtg cccccaagac cgtcgagcga ttcatggttg cggtcaacgc cgggaagtac
     4081 gacgggctca agttccatcg catcatcaag gacttcatgg tccagggcgg cgacccgcag
     4141 ggcaacggca tgggcggggg caatgttcag tatgagggct cagacgtcaa acatgtccct
     4201 ggcgtcatca gcatggcagc gcagcaggcg cacgtcgacc agtccgacat gcagttcttc
     4261 atcatgacca gcacatccag ccagctggat ggcaactaca ccgcgttcgg acatgtcacc
     4321 gaaggcatgg atgtcctggc ggccattgca gcgacgcctg tcgcgcaggc gccgtggggc
     4381 gagcgttccc gtccggtgga ggacgtgcgc atcgtcaagg cttccgagat cccggaatag
     4441 cacagagagg gggagcttat ggccattcac atcgtgacgg actccacaag ctgccttccc
     4501 actggtttcg ccgagcagca cggccttccc gtcgtgttgc tcaaggtgtc ggtcgacgga
     4561 gtcttcttcc gcgagttctc ggagatagct cccgatgtct tctatgcaaa gcagag
//
LOCUS       DATMZD010000143         4888 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_144763,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000143 DATMZD010000000
VERSION     DATMZD010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4888
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144763"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..1232
                     /locus_tag="VN478_03005"
     CDS             150..1232
                     /locus_tag="VN478_03005"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HWQ21678.1"
                     /translation="MENEYYGDRIHRLQGRMAGANVDAFFTLAAPNMRYLTGFTGEEG
                     YLLALSDGIHLVVDGRFTTQAREECPWVDIIEYTGQFSRSIAGLLVGSHAHWLGFERE
                     RVSFVQAEQMRAAFPSVNMVPTENVIEGMRIVKDDRELDVMRTAGHVADKAFSAMITQ
                     LKAGMTENEVAALLEYEMRKAGAQGTSFATIVGSGARGALPHGIASGKVIENGDVIVI
                     DFGVNYEGYMSDCTRTVALGEQPADVNDVYGKLRTAQQLGLDCIRAGVSSRSVDTAVR
                     GNLGESGLAQYFTHGLGHGVGLEIHEAPRLSQGTDTVLEPGQVVTCEPGVYLPGQYGM
                     RIEDSVIVTATGFEPLTALPKELVLA"
     gene            1336..1896
                     /gene="efp"
                     /locus_tag="VN478_03010"
     CDS             1336..1896
                     /gene="efp"
                     /locus_tag="VN478_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HWQ21679.1"
                     /translation="MISANELRTGVTFEIDGQAFSVIQFTHIKPGKGSPFVRLKMKNL
                     MSGNVVEQTFRPDEKVKSAYLEHKKMQYLYDTDGVYTFMDSATYEQIGLTKDDLGDSV
                     NYLIENIVVDVVYFLEKPVSVELPTFIEAVITHTEPGIKGDTATGANKTAQIETGFSL
                     MVPLFVNEGDKIRIDTRSGDYLERVK"
     gene            1901..2224
                     /locus_tag="VN478_03015"
     CDS             1901..2224
                     /locus_tag="VN478_03015"
                     /inference="COORDINATES: protein motif:HMM:NF015721.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HWQ21680.1"
                     /translation="MDKVLITDAALESLIVHACLSTEGVESMWKGLKEHFPFWDREGR
                     EPHGVSITRNGLELTLDVFIVVKLGADLRKFATSVRCAIAKEVEASTPYHIQEVNVHI
                     ADVRA"
     gene            2228..2644
                     /gene="nusB"
                     /locus_tag="VN478_03020"
     CDS             2228..2644
                     /gene="nusB"
                     /locus_tag="VN478_03020"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HWQ21681.1"
                     /translation="MTTTHGVQNRRRSREIALQVLYSLAQGNASYEQTFDLYAHEAPL
                     VLAYALKLIQGVQAHQDAILDEVGAHSANWGIDRVAVVDRVILSIAVFEMKYGKPPIK
                     TAIAANEAVELAKEFGGAESGSFVNGVLRGIGGAGD"
     gene            2659..2919
                     /locus_tag="VN478_03025"
     CDS             2659..2919
                     /locus_tag="VN478_03025"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HWQ21682.1"
                     /translation="MKSLVADVSGIVQGVGFRHVVEQRAAQLGIDVRADNMDDGTVHI
                     IASGDEERLLRLVEVLRQGSRYSSVDRVDYTITDLRERNEGS"
     gene            2921..3811
                     /locus_tag="VN478_03030"
     CDS             2921..3811
                     /locus_tag="VN478_03030"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF045485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HWQ21683.1"
                     /translation="MDIRDHLNEEAHYIDGVLADCLAPLQGSVMHAAMSYALLSPGKR
                     VRPALCLAVYEACTRDRERIKPVLAAIEMVHAYSIIQDDLPSMDNSDLRRGKPTTHKV
                     HGDDIALLASDALLTLAFGELSSAQMVSDFGSERCLEAVRTLAGLAGAEGLVGGQAMD
                     IMTSGDEHVELGTIEYIHKNKTAALIQASVLLGGIFGCASEPDMDRLRAFGENFGMAY
                     QIVDDIADVTASEAQTGKPTGQDVKNRKATYVTALGLADAREKALQYLARARESALAL
                     DSGGDFLTGLVDLLAAKARS"
     gene            3808..>4888
                     /locus_tag="VN478_03035"
     CDS             3808..>4888
                     /locus_tag="VN478_03035"
                     /inference="COORDINATES: protein motif:HMM:NF024688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HWQ21684.1"
                     /translation="MILDHVASPADVRGLDIAELRTLAAECRQVILDTCSTNGGHLAS
                     NLGAVELTIALHVTLDTPVDQLVWDVGHQCYTHKLLTGRYATFSTLRQCGGVSGFVNP
                     NESPYDTFMAGHASTSLSIVAGLLAARRLNGGNQRVVAVIGDGALSGGMALEALNNLG
                     RARGQCLVILNHNDMSISRSVGGMARALNRLRTRAWYLRAREAPLVGRGLRWLRTLLK
                     RLYLPTIFFEELGFRYFGVVDGHDIGKLIEAIDMVKDIQQPVVLQVVTVKGKGYELAE
                     RQPSQFHGVAPFLIENGLSKQEKSMPTYSDVFGEALVRLAETDSRIVAITAAMMDGTG
                     LAEFGRRFPDRCYDVGIAEQHAVGMA"
BASE COUNT          988 a         1368 c         1610 g          922 t
ORIGIN      
        1 gttccaggtg gctcagcaca aggacctgga cgtcgtaaat gcacagctca agacgaggaa
       61 gacgttcctg accctgacac ctcagccgat tcctgcggag acaggcaagg ccaacccatt
      121 cgaatagtac ggtctggagg agacgaacca tggaaaacga atactacggt gacaggatcc
      181 atcgattgca ggggcgcatg gccggtgcga acgtggatgc attcttcaca ctggcggcac
      241 ccaatatgcg ctatctcacg ggcttcactg gcgaggaagg gtatctgctt gcgttgagtg
      301 acggcataca ccttgtcgtc gacgggcgat tcacgacgca ggcgcgcgaa gagtgcccgt
      361 gggtcgacat catcgagtac acggggcagt tcagtcgatc gatcgcgggc ttgcttgtgg
      421 gcagccacgc gcactggctc gggtttgaga gggagcgcgt ctccttcgta caggccgagc
      481 agatgcgggc cgcgttcccc tcggtgaaca tggtcccgac ggagaacgtg atcgagggca
      541 tgcgtatcgt caaggacgat cgtgagctgg acgtgatgcg taccgcgggg cacgttgcag
      601 acaaggcctt tagcgcaatg atcacgcagt tgaaggccgg gatgacggag aacgaggttg
      661 ctgccctgtt ggagtacgag atgcgcaagg cgggcgcaca aggaaccagc ttcgctacga
      721 tcgtggggtc aggtgcccgg ggggcattgc ctcatgggat cgcctccggc aaggtcatcg
      781 agaacggcga cgtcatcgtc atcgactttg gtgtcaacta cgaagggtac atgagcgact
      841 gcacgcggac cgttgcactg ggtgaacagc ctgccgacgt gaacgacgtc tatggcaaac
      901 tgcgaactgc gcaacagctg ggtctggact gcatcagggc gggcgtgagc tcgcgctcgg
      961 tggacaccgc tgtaaggggc aacctcgggg agagcgggct ggcacagtac ttcacgcatg
     1021 gtctcgggca tggcgtcggg ctggagatcc acgaggcgcc tcgcctgtcg caggggaccg
     1081 atacggtgct tgagccggga caggtcgtga cctgcgagcc tggcgtctac cttcccggac
     1141 aatatggcat gcgcattgag gattcggtca tcgtgacggc tacggggttc gagccattga
     1201 cggcactgcc caaggagctg gtgctcgcct gagacccagg gagtgtggcg gcgcagacag
     1261 cattaacgct tgacgcaacg cttgttgccg tgtacaatag ccgcgattga cagccgagta
     1321 ccaggaggtt agacattgat ttctgcaaat gaactgcgca caggtgtcac gtttgagata
     1381 gatggtcagg cgttttcggt catccagttc acgcacatca agcccggcaa gggttctccg
     1441 ttcgtccgtc tcaagatgaa gaacctcatg agcggcaacg tcgtggaaca gacattccgt
     1501 cccgacgaga aggtcaagag cgcgtatctc gagcacaaga agatgcagta cctgtacgat
     1561 acggatggcg tctacacctt catggactcc gcgacctacg agcagatcgg cctgacgaag
     1621 gacgaccttg gcgattcggt caactatctc atcgagaaca ttgtcgtcga cgttgtctac
     1681 ttcctggaga agccggtaag cgtggagttg cctacgttca tcgaagcagt catcacgcat
     1741 acggagccgg ggatcaaagg cgacactgcc acgggtgcga acaagaccgc tcagatcgag
     1801 accgggttct cacttatggt cccgctattc gtgaacgaag gcgacaagat tcgcatcgat
     1861 acacgcagcg gagactacct cgagagggtc aagtaggtct atggacaaag tcctgatcac
     1921 cgacgccgcg ctggagtccc tcatcgtaca cgcctgtctg tcgacagagg gcgtggaatc
     1981 gatgtggaag ggcctcaagg agcacttccc gttctgggac cgtgaaggcc gggagccgca
     2041 cggggtcagc atcacgcgca acggcctcga gttgacgctc gacgtgttca tcgtcgtgaa
     2101 gttgggggcg gatctccgga agttcgccac cagcgtacgg tgcgcgattg ccaaggaagt
     2161 ggaagcctct actccatacc atatccagga ggtcaacgtc catatcgctg acgtgcgggc
     2221 ctgacacatg acgacaactc atggggtcca gaaccgccgg cgttcgcggg agatagcgct
     2281 gcaggtcctg tacagcctgg cgcagggtaa tgcctcgtat gaacagacgt ttgacttgta
     2341 tgctcatgaa gcgccgctcg tgctggctta tgcgctcaag ctcatccagg gagtccaggc
     2401 tcatcaggat gcgatcctgg acgaggttgg tgcgcacagt gcaaactggg gcatcgacag
     2461 ggttgccgtg gtggaccgtg tgatcctctc catcgccgtc ttcgagatga agtatggcaa
     2521 gcctccgatc aagactgcca ttgccgccaa cgaggcggtc gagcttgcca aggagttcgg
     2581 tggtgctgag tctgggagct tcgtgaacgg cgtcctgcgt ggtatcggag gcgccggaga
     2641 ctgaccgagc aggtgtcctt gaagagtctg gtcgcggatg tatctggcat tgtgcaggga
     2701 gtcgggtttc gacacgtcgt ggagcagcgg gcggctcagc tcgggatcga cgtcagggct
     2761 gacaacatgg atgacggtac cgtgcatatc atcgcctcgg gcgacgaaga gcggctcttg
     2821 cgcctggtag aagtgctcag gcaggggagc cgctattcat ctgtggaccg tgtcgactac
     2881 acgatcacgg atctgcgtga acggaacgaa gggagctgac atggacatca gggatcatct
     2941 caacgaagaa gcgcactata tcgacggagt gctggccgat tgtctggctc cacttcaggg
     3001 atccgtcatg catgcggcca tgtcctacgc gctgttgtcg ccgggcaagc gggtgcgccc
     3061 ggccctgtgc ctggcggtat acgaggcttg tacccgcgac agagaacgca tcaagccggt
     3121 gctcgcggca atcgagatgg tacatgcgta ttccatcatc caggacgatc tgccctcgat
     3181 ggacaatagc gacctgcggc gaggcaagcc gacgacgcac aaggtccacg gggatgacat
     3241 agcgctcctg gcctcggacg ccctgctgac gctcgcgttc ggagagcttt cgtctgcgca
     3301 gatggtcagc gattttggct cggagcggtg ccttgaggcc gtgcggacgt tggctggcct
     3361 tgcgggagcg gaaggtcttg tcggcgggca ggcgatggac atcatgacct cgggcgatga
     3421 acacgtcgag ctggggacga ttgagtatat ccacaagaac aagactgctg cgcttatcca
     3481 ggcctcggta ctgctcggcg gcatctttgg ctgtgccagc gagccggaca tggacagact
     3541 gcgtgcattt ggcgagaact tcggcatggc gtaccagatc gtggacgaca tcgcggacgt
     3601 caccgcgagc gaagcccaga ccggcaaacc cacaggccag gacgtcaaga acaggaaggc
     3661 aacctatgtc acagcgctgg ggctggcgga tgccagagag aaggcgctgc agtacctggc
     3721 gcgcgcgcgg gagtctgcgc tggcactgga cagtggggga gacttcctga ccggcctagt
     3781 cgaccttctt gccgcgaagg ctcggagttg atcctggacc acgttgcctc tccggccgac
     3841 gtccgcgggc tcgatattgc cgagctgcgg actcttgcgg cggagtgcag gcaggtcatc
     3901 ctcgacacgt gttcgacgaa tggaggacat cttgcgtcga acctcggtgc cgtcgaactg
     3961 acgatcgccc tgcacgttac gctggacacg cctgtggacc agctggtgtg ggacgtggga
     4021 caccagtgct acacgcacaa gctcctcacc ggcaggtacg cgacgttcag cacgctgcgg
     4081 cagtgcgggg gtgtcagcgg tttcgtgaat cccaatgagt ctccctacga cacgttcatg
     4141 gccggacacg cttctacctc cttgtccatc gtcgccggtc tgctggcggc ccggcgcctg
     4201 aacggcggga accagcgtgt ggtcgcagtc atcggggacg gagccctgtc gggcggcatg
     4261 gccctggaag cgctcaacaa cctcgggcgg gcacgcggcc agtgtctcgt catcctgaat
     4321 cacaacgaca tgtccatctc ccgctccgtg ggggggatgg cgcgagcgct caaccgtctg
     4381 cgaacgcggg catggtatct gcgcgcgcgc gaggccccgc tggtggggcg cgggctccgt
     4441 tggctgagaa ccctgctgaa gcgactgtac cttcccacga tcttcttcga agagcttggt
     4501 ttccggtact ttggagtcgt cgacgggcat gacatcggca agctgatcga agcgatcgac
     4561 atggtcaagg acatccagca gcctgttgtc cttcaggtcg tgacagtgaa gggcaaggga
     4621 tacgagctgg ccgaacgcca gccgtcgcaa ttccacggcg tggcgccgtt tctcatcgag
     4681 aacggattga gcaagcagga gaagtcgatg ccgacgtact cagacgtatt cggtgaggcg
     4741 ctcgtgcgac tcgcggagac cgattcccgc atcgtcgcga taacggctgc gatgatggac
     4801 ggaacgggtc ttgccgagtt tggcaggcgc tttcccgacc gctgctacga cgtcggcatt
     4861 gccgaacagc atgcagtcgg catggccg
//
LOCUS       DATMZD010000144         5594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_4099,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000144 DATMZD010000000
VERSION     DATMZD010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5594
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4099"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="VN478_03040"
     CDS             <1..478
                     /locus_tag="VN478_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HWQ21685.1"
                     /translation="LNGLLDACRDEGISTAVETCGMTDQGELLGMGDRVDLFLYDVKV
                     MDTARHSEATSVGNQTILTNLAALAAVHAHVVVRFPVIPGVNDDKDNVDLMVEMMCRL
                     GLREIDLLPYHRIGTDKYRRLGRLYEMAELQPPSAEVMEGIRRIFADAGIEARVGG"
     gene            480..2837
                     /gene="hypD"
                     /locus_tag="VN478_03045"
     CDS             480..2837
                     /gene="hypD"
                     /locus_tag="VN478_03045"
                     /EC_number="4.2.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014186229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019471 - 4-hydroxyproline metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-4-hydroxy-L-proline dehydratase"
                     /protein_id="HWQ21686.1"
                     /translation="MNRRVARLRQESLAAVPRLSLERAHLVTEFDRQARGLSVPVYRA
                     RLLQYILEHKQLFIGPGELIVGERGPEPKAVPSFPELCCHSMQDLTVLDTREKISYKV
                     DDEGRRIQQEEVIPAWNGRSMRDRMFALMEPAWKDAYEAGVFTEFMEQRAPGHTVLGD
                     VIYQKGLLDLKTEVEEAIAGLDFLNDPQATARREELRAMAIAADAMIGYAGRNASLAR
                     SMADVETDPARRAELLDIADVCSWVPAHAPRRFREALQAYWFVHLGVVTELNTWDSFC
                     PGRLDQHLEPFYARGLADGTLTRESAMELLECFWIKFNNQPAPPKVGVTAAESGTYTD
                     FANINVGGLCPDGSDGVNDVSYLLLDVIDDMHLLQPSSNIQLSRKSPDRFLRRAAAII
                     REGWGQPSVFNADMVVEELLRQGKSVVDARMGGISGCVETGAFGREAYILTGYLNLPK
                     ILEVTLHDGLDPRTGKVIGLRTGDPRSFASYDELFEAFKAQLHHFVDIKVRGNNVIER
                     LYAEQMPAPFLSLFVEDCIARGRDYNDGGPRYNSTYIQVAAPGSCSDSLTALKAHVFD
                     RKDLTMDEIMAAVDADFVDRERTRLLLWNRSPKYGNDDPAADDILARLFDVVFEAIDG
                     RPNTKGGTYHVNYLSTTVHVYFGSVTGAMPDGRRAWQPLSDGISPVQGADRKGPTAVI
                     QSAARMDHVRTGGTLLNQKFSPSLVEGEVGLDALTHLVRSYFTLGGHHIQFNVVTAAT
                     LRAAQAEPEKYRDLIVRVAGYSDYFCDLTSGLQEEIIARTEQQTF"
     gene            2846..3715
                     /locus_tag="VN478_03050"
     CDS             2846..3715
                     /locus_tag="VN478_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013624571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWQ21687.1"
                     /translation="MHYKDAKSILSSRNGMNLYRGCTHGCIYCDSRSRCYQMEHAFED
                     VEIKRNAPELLEAALRRKRHRCMIGTGSMSDPYLPIEEQERLTRRCLEIVDRHGFGFT
                     AQTKSDRILRDLDLLKSINRKARCVVQMTLTTYDEELCRILEPNVCTTRRRFEVLKTL
                     RDEGIPTVVWLCPILPFINDTEENLCGILRYCFEAGVHGIVCFGMGVTLREGDREYFY
                     AKLDEHFPGMKERYVHTFGDRYECSSPRNAALMKVLHDECSARGVLCEPGEVFAYLSE
                     SEAAWAGGQQSLF"
     gene            complement(3730..4596)
                     /locus_tag="VN478_03055"
     CDS             complement(3730..4596)
                     /locus_tag="VN478_03055"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HWQ21688.1"
                     /translation="MTDGHIARQVKAERIIVAVLFGAAGLLIVAVFSPWQPLLSGLMD
                     TLGRVAVMISLLTAALLVRRSPRLNRYWRLLYGLFVLAAAVSADWFAASALIRMSVLD
                     PGAPMGMAFQKLADGIVISAVVLLLTRISGDSIGSIYVQKGHLRRGIIVGMVTFCMAA
                     ATSVPIAQLMFRAQNLTMSRMLPWIPWILIFILASATDEEIMFRGLFLRKLEPFFGPF
                     MANCLIALVFTGLHAAVTYTSSQMGFLAVLLPLALAWGYVMQKTDAVWGSILFHAGMD
                     IPIVLGLFSTAS"
     gene            complement(4593..4970)
                     /locus_tag="VN478_03060"
     CDS             complement(4593..4970)
                     /locus_tag="VN478_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21689.1"
                     /translation="MEHARGRFRFVWIPRALMILFILFLTMFSFDVFEMEGTFLAKLG
                     GFFMHSIPSLVLVAVLLVAWRNPLVGGILILGSAAALMLRWHLWRGMTFVTIILPIIV
                     VGVLFIVSHVLDRKSSPIEGGQA"
BASE COUNT         1175 a         1652 c         1747 g         1020 t
ORIGIN      
        1 cctgaacggc ctgctcgatg cttgccggga cgaaggcatt tcgactgcag tcgagacctg
       61 cgggatgacc gaccaggggg aattgctggg gatgggggac agggtcgacc tgtttctgta
      121 cgacgtcaag gtgatggata cagcgcgaca tagtgaagca accagtgtgg gaaaccagac
      181 gatcctcacc aaccttgcag cgctggcagc agtccatgct cacgtcgtcg tacgattccc
      241 cgtgatcccc ggcgtcaacg acgacaagga caacgtcgat ctgatggtgg agatgatgtg
      301 tcggcttggg ctgcgcgaaa tcgacctgct gccatatcac cgcatcggaa cagacaagta
      361 tcggagactt ggaaggctgt acgagatggc cgaactccag cctccttcag cagaagtgat
      421 ggaaggcatc cgacgcatat tcgcagacgc cggcatcgaa gcgagagtgg gaggttgaca
      481 tgaataggcg cgttgcccgt ctgcgtcagg agagccttgc cgcagtgccc cggctatccc
      541 tggagcgggc gcatctggtg acggagttcg accggcaggc gcggggattg tcggtgcctg
      601 tctaccgcgc acgcctcctg cagtacatcc ttgagcacaa gcagctgttc atcggacccg
      661 gcgagctgat cgtgggcgag cgcggtcctg agccaaaggc cgttccgtcc tttcccgaac
      721 tgtgctgtca cagcatgcag gaccttactg tcctcgacac gcgggagaag atctcctaca
      781 aggtcgacga cgagggtcgc cgcatccagc aggaggaggt catccctgcg tggaacggcc
      841 ggtctatgcg cgaccgcatg tttgctctca tggaacctgc gtggaaggat gcctatgaag
      901 ccggcgtgtt caccgagttc atggagcagc gtgcgccagg ccatacggtc ctgggagacg
      961 tcatctatca aaagggtctg ctggacctga aaacagaggt ggaagaagcc attgcgggtc
     1021 tcgacttcct gaacgacccg caggcgaccg cgcgtcgcga ggagctgcgc gccatggcca
     1081 tcgccgctga cgccatgatt ggctatgctg gaaggaacgc ctccctggct cggtcgatgg
     1141 ccgacgtgga gacggacccg gcgcgcagag cggagcttct ggacattgcc gacgtgtgct
     1201 catgggtgcc ggcacatgct ccacgcaggt tccgcgaggc gctgcaggca tactggttcg
     1261 ttcatctggg tgttgtgacc gagctgaaca cctgggattc gttctgtcct gggcgcctcg
     1321 accagcacct ggagccgttc tacgcaagag gactcgccga cggtacgctg acgcgcgagt
     1381 cggccatgga actcctggag tgcttctgga tcaagttcaa caatcagcct gcgccaccaa
     1441 aagtgggcgt gacagctgcc gaaagcggga catacacgga cttcgccaat atcaatgtag
     1501 gtggactgtg cccggacggt tcagatggcg tgaacgacgt cagctacctc ctgctcgacg
     1561 tcatcgacga tatgcacctg ctgcagccgt cctccaacat ccagctcagc cgcaagagcc
     1621 cggaccgctt tctgcggcgt gctgctgcaa tcatccgcga aggctggggt cagccctctg
     1681 tcttcaatgc cgacatggtc gtcgaggagc tcctccgcca gggcaagtcg gtggtcgacg
     1741 ctcgcatggg gggtatctcc ggctgtgtcg aaacgggtgc cttcggacgc gaagcgtaca
     1801 ttctcacggg ctacctgaac ctgcccaaga tccttgaagt gaccctgcat gacggtctgg
     1861 acccacgtac aggcaaggtc atcggtctgc gcacgggtga tccgcggtca tttgccagct
     1921 atgacgagct gtttgaggca ttcaaggccc aactgcatca cttcgtggac atcaaggtgc
     1981 gtggcaacaa cgtcatcgag cggctgtatg ccgagcagat gccggcgccg ttcctgtcgc
     2041 tgttcgtgga ggactgcatc gcgcgggggc gtgactacaa cgatggtggc ccgcggtaca
     2101 actcgaccta catccaggtc gcagcgccgg ggagttgttc cgacagcctg acggcgctga
     2161 aggcgcatgt ctttgaccgc aaggatctga cgatggatga gatcatggca gccgtcgacg
     2221 ccgatttcgt ggaccgtgag agaacgcggc tcctgttgtg gaaccgctca cccaaatatg
     2281 gcaacgacga cccggctgcc gacgacattc tggcccgact gttcgacgtc gtctttgaag
     2341 cgatcgacgg ccgccccaac accaagggcg ggacgtatca tgtgaactac ctgtcgacaa
     2401 cggttcacgt gtacttcggt tcggtcacgg gcgcgatgcc cgatggccgc cgggcgtggc
     2461 agcccctgtc ggatggcata tcgcctgtcc agggagcgga caggaagggc ccgaccgccg
     2521 tcatccagtc ggcggctcgc atggaccatg tgcgtactgg gggtaccctc ctcaaccaga
     2581 agttctcgcc atcactggta gaaggagaag tcggactgga cgcgctcaca cacctggtcc
     2641 gctcctactt cacgctgggc ggacatcaca tccagttcaa cgtcgtcacg gctgcgactc
     2701 tgcgtgctgc ccaggccgag ccggagaagt accgcgacct catcgtgcgt gtcgccggct
     2761 acagcgacta cttctgtgac ctgacgtccg gtctgcagga ggagatcatc gcgcgcaccg
     2821 agcagcagac gttctagcga agtccatgca ctacaaggac gcaaagagca tcctgtcgtc
     2881 caggaacggc atgaacctct accgcggttg tacgcacggg tgcatctact gcgactcccg
     2941 cagcaggtgc tatcagatgg agcacgcgtt cgaggacgtg gaaatcaagc gcaacgcgcc
     3001 ggagcttctg gaagctgccc ttcgccgcaa acggcaccgc tgcatgatcg gcaccggctc
     3061 catgagcgat ccgtacctgc ccatcgagga gcaggaacgg ttgacccggc ggtgccttga
     3121 gatcgtcgac cggcatggct tcggtttcac agcccagacc aagtctgacc ggatcctccg
     3181 ggacctggac ctgctgaaga gtatcaacag gaaagccaga tgtgtcgtac agatgacgct
     3241 gaccacgtat gacgaggaat tgtgccgcat ccttgagccc aacgtctgca cgacccgccg
     3301 acgtttcgag gtgctcaaga ccctgcggga cgagggcatc cccacggtgg tatggctgtg
     3361 tccgatcctc ccgttcatca atgacacgga ggagaacctg tgcgggatcc tccgctactg
     3421 ctttgaggcc ggcgtccacg gcatcgtctg ctttggcatg ggcgtaacat tgcgtgaggg
     3481 cgaccgggag tacttctacg cgaagctgga cgagcacttt cccggcatga aggagcggta
     3541 tgtccacacg tttggcgaca ggtatgagtg cagcagtccc cggaacgccg ctctgatgaa
     3601 ggtcctccat gacgagtgca gcgcccgagg agtcctctgc gagccgggag aggtgttcgc
     3661 ctacctcagc gagtccgaag ctgcatgggc cgggggacag caaagcctgt tctgaccgag
     3721 aaagtcaggc tagctggccg ttgagaacaa gccgaggacg atcgggatgt ccatgccggc
     3781 gtggaacagg atggagcccc acactgcgtc agtcttctgc atgacatagc cccaggcgag
     3841 tgccaacggg agcaagaccg caagaaagcc catctgactg cttgtgtagg tgacggcagc
     3901 gtgcaggccg gtgaacacga gggcgatcag gcaattcgcc atgaacgggc cgaagaacgg
     3961 ttccagcttg cgcagaaaga ggccacggaa catgatctcc tcgtcggtgg cgctcgccag
     4021 gatgaagatc aggatccacg gaatccaggg cagcatgcgt gacatggtca ggttctgcgc
     4081 acggaacatg agctgggcga tcgggaccga tgtggccgct gccatgcaga aggtgaccat
     4141 gccgacgatg atcccgcgcc tcaagtggcc tttctggacg tagatggagc caatactgtc
     4201 gcctgagatc ctcgtcagca ggagaaccac agccgagatc acgataccgt cagccagctt
     4261 ctggaatgcc atgcccatgg gggctcctgg gtcgaggaca ctcatgcgga tcagcgcact
     4321 cgcggcgaac cagtctgcag agacggcggc ggccagcacg aacaagccat acagcagccg
     4381 ccagtacctg ttgagccgtg gactgcgccg tacgagcagc gcggcggtca gcagcgagat
     4441 catcacggcg actctgccca gtgtgtccat gagtcctgac agcagtggct gccacggact
     4501 gaagacagcg acgatcaata gacctgcagc gccgaacaga acggcgacga tgatgcgctc
     4561 cgccttcact tgtctggcga tgtggccatc agtcacgcct gtcctccttc tatcggcgac
     4621 gacttcctgt ccaggacgtg cgacacgatg aacaggacac cgacgacgat tatgggaagg
     4681 atgatggtca cgaatgtcat gcctctccac aggtgccagc gcagcataag cgctgcggcg
     4741 gacccgagaa tcaggatgcc gccgacaagt ggattcctcc acgcgaccag caggacggct
     4801 accagaacca gcgaggggat ggaatgcatg aagaacccgc ccagcttggc gaggaacgtc
     4861 ccctccattt cgaagacatc gaatgagaac atcgtcagga acaggatgaa caggatcatg
     4921 agagccctgg gaatccagac gaatctgaat cgaccgcgtg catgttccat tgccttcacc
     4981 tcccttctcg tacgtcacaa gacctcgcac cagagaggat ccaatcgacc ggccgtcctg
     5041 gctcgaacgc gaagcctgtt gcggatcaca ttccagacgc agtggtacgc taaggccatt
     5101 gtcatccttt ccaggaacag tacaactcct cgtactggag catactgaca accgtcggac
     5161 tccgtcgaga gtgtcacggg aggtttgaca ctgtacggag cagacggggt ttctgcgccg
     5221 gtgaggggtt ggacctctgc gccacagccc atgaaccgga ttcaccttga gttggcgggc
     5281 gtcggaggcg ccgttgacgg tgacttcagg cagagtcagg caagcgaaga cactggacac
     5341 atgaagacgg tttccgaatt gggggcacaa tcgaggcaca atagtcatga ggctcgccac
     5401 tcacgtcagg ccgtctcatc tatcctgttg cgcaaaggag tgaagggatg ctgcagaaga
     5461 agggtaccag tcgctcatct tccaggagtc acttgcctgt cgtccggggc gtcgtcgttc
     5521 tgggtgtcgt cgcagttctc tgggtcggag gcgtcgttgc tgcacacgga cacggaagcc
     5581 ctgagaacat catc
//
LOCUS       DATMZD010000145         2040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_84646,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000145 DATMZD010000000
VERSION     DATMZD010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84646"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..955
                     /locus_tag="VN478_03065"
     CDS             116..955
                     /locus_tag="VN478_03065"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HWQ21690.1"
                     /translation="MTYSVALAQVNPVCGDLEYNVKLHIAQAEQALEVGAAMIVFPEL
                     SLTGYSLKDQTLDIMRHADCPEFAGLRALSRHIAVCAGFVEEGGDGIPYNSAFFMDKG
                     EIQHVHRKMYLPTHGMFEELKFLGHGRELSVFDTRFGRAGIVICRDLFHPLEVSTMAS
                     LGVELLLAPSSIPARGFSGEELAIEHTVGLALGSASTFLGMFVVYVNRVGFDDGLGFY
                     GGSALSDPVGGITVSAPRFETAQVLGTIDTSHIARRRYQLPILREENLEIVRHALETR
                     RDS"
     gene            952..1779
                     /locus_tag="VN478_03070"
     CDS             952..1779
                     /locus_tag="VN478_03070"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HWQ21691.1"
                     /translation="MTPDVLALNNVLVYDYLKRFVQEELHTSGFTEASIALSGGLDSA
                     LVLKLAVDALGADHVHAIFLPYKASSDHSRRDARTMFEFCGIPWQELDISSMADAYIA
                     LQPGIDRLRTGNLLARLRMAVIFDQSKQENALVVGTSNKSELLVGYSTWYGDMACAIM
                     PIGDLYKTQVRSLARHIGLPEQVIAKPPSADLWAGQTDEGEIGVSYETLDQILYRLVD
                     QRCTAAEAVSEGIELDMVNRIEHKILMSQFKRVPPPIAKLSQRTVGTDFLYPRDWRK"
BASE COUNT          409 a          609 c          594 g          428 t
ORIGIN      
        1 gctacatgcc ggtagccatg acgatcctgg ccggcgaagc agctgtcgtg attcttcttg
       61 gcattcctgc gcttgtcctc gttcgtcaca acatcaaccc cgtcaaggag gcccgatgac
      121 ctactccgtt gctcttgccc aagtgaatcc cgtctgtggc gacctggagt acaacgtgaa
      181 gctgcacatt gcccaagccg agcaggcact tgaggtgggc gctgctatga tcgtcttccc
      241 tgaactgtca ctcaccgggt actcactcaa agaccagaca ctcgacatca tgcgtcatgc
      301 cgactgcccc gaatttgcgg gactacgcgc gctctcccgc cacattgccg tctgtgcggg
      361 attcgtggaa gaaggaggtg acggtatccc ctataacagt gccttcttca tggacaaggg
      421 ggaaatccag cacgtccacc gcaagatgta cctgcccacg catggcatgt tcgaggaact
      481 taagtttctt gggcatggca gagaactgtc ggtcttcgac acccgctttg ggcgggccgg
      541 catcgtcatc tgccgcgacc tctttcatcc gctcgaagtg tcgacgatgg cgtctctggg
      601 cgtagagttg ctgctggcgc cgagctcgat acctgcgcgt ggcttcagcg gcgaagaact
      661 ggcaatcgag cacacggtag gcctggctct tgggtcagca agtacctttc tggggatgtt
      721 cgtcgtctat gtgaaccgtg tgggatttga cgacgggctt ggtttctatg gggggtctgc
      781 tctgagtgac ccggtcggag gcatcacggt ctcggctccc cggtttgaga ctgcacaggt
      841 tctcgggacc atcgatacgt cgcacattgc acgacgccga tatcaactcc ccattctccg
      901 cgaggagaac ctggagatcg tacgccatgc actggaaacg aggagggact catgacgccg
      961 gacgtccttg cactcaacaa cgtactcgtc tatgactatc tcaagcggtt tgtccaggaa
     1021 gagctgcata caagcgggtt cacagaggcg tccattgccc tgtcgggagg cctggattcc
     1081 gcattagtgc tgaagctggc agtcgacgct ctcggagccg accatgttca cgccatcttc
     1141 ctcccctaca aagccagctc agaccacagc agacgagatg cccgtaccat gttcgagttc
     1201 tgtggtattc cctggcagga gctggacatc agcagcatgg ccgatgcata tatcgcgctg
     1261 cagcctggca tagaccggct gcggactggc aacctgctcg ctcgcctgcg catggcggtg
     1321 atctttgatc agtcgaagca ggagaatgcc ctcgtcgtcg ggacaagcaa caagagcgaa
     1381 ctgctggtcg gctactcgac ctggtatggc gacatggcct gcgccatcat gcccattggc
     1441 gacctgtaca agacgcaggt acggtcactg gcaaggcata tcggactgcc agagcaggtc
     1501 atcgccaagc ctccttcggc cgacctttgg gccggtcaga ccgatgaggg agagatcggc
     1561 gtgagctatg agacgcttga tcagatcctg taccgcctgg tcgatcaacg gtgtacggct
     1621 gcagaagcgg tatcagaagg catcgaactc gacatggtga accgaatcga acacaagata
     1681 ctgatgtccc agttcaagcg cgtgcctccg cccattgcca agctttcgca gcgcaccgtc
     1741 ggcaccgact tcctgtaccc gcgtgattgg cggaagtagc ctgcatcgta gaaggctgtg
     1801 acacgccaga cccccggcca tccggccggg ggtctgcttt tcgccgagct gtgcctttag
     1861 ccgggcaaag tctagtagaa ggttttggcc tgaaggacga catccgcggg gatgacatgc
     1921 ggctgcgtcg tcgcttccct gaggtcagta aaagcaattc gctgtccgac ctcgcccaca
     1981 agaatcccgg acttgccttc acgcactgcg ttcacggcgg cgaggcccag tcgggtagcc
//
LOCUS       DATMZD010000146         2211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_44048,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000146 DATMZD010000000
VERSION     DATMZD010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44048"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VN478_03075"
     CDS             complement(<1..435)
                     /locus_tag="VN478_03075"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWQ21692.1"
                     /translation="MYVQEVGPAGAPTIVLLHGAGVGGWMWRDTIAALPDVHVLVPDM
                     PEQGQSHDAGLFSIAGTARLVADLIAERAHGGEAFVAGLSLGAQVTVALLGCRPGVVL
                     GAFASGTLVRPIAGVGLVSAMLPLYMPMRNARWLVRANMKALE"
     gene            complement(609..1745)
                     /locus_tag="VN478_03080"
     CDS             complement(609..1745)
                     /locus_tag="VN478_03080"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HWQ21693.1"
                     /translation="MKSSYDCVIIGGGISGLAIAYELAKRGYRDVAVLDQCYLGSGST
                     GRCGSGIRAQFTGPDQITLLREAEEMWARMSDELGYDIHFKACGYLFLWDTPELVEKA
                     KQNVAFQNKHGVMSRIVDRQEAKEICPSLNTDSFLAGQWYDKDGMAFPFDTVTGYAQA
                     ARRLGVDILTTTDVFGLETTPEHTFTVHTTQGNIEAKNVVNAAGPGSKPIAAMLGVDL
                     PYRLDKHQIMATEPLEQFLGPMLVKGSLYYLQTYRGNIVGGTDTGEKGATNIDSTFEF
                     LTKFSREATEIVPRLAGVKLMRQWAGYYVVSPDAHPVLGPVDEVPGFFQANGYSGHGF
                     MMGPIVGRLLAEYIADGKPSIDIEHYNFRRFARGELLTEKAVIG"
     gene            complement(1735..2028)
                     /locus_tag="VN478_03085"
     CDS             complement(1735..2028)
                     /locus_tag="VN478_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011838314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HWQ21694.1"
                     /translation="MTKEDEKTIACRCEDISVQEIKDAIHAGYADLQSLKRYAKFGLG
                     PCQGKTCILLAAQILARETGRTLDQIMTPTTRPPVDPVPLGFFVKGKEVDDEK"
BASE COUNT          435 a          690 c          667 g          419 t
ORIGIN      
        1 ctccagggcc ttcatgttgg cccgcaccag ccaccgggca ttccgcatcg gcatgtacaa
       61 cggcagcatc gcgctcacga gcccgacgcc ggcgatgggc cgcacgagcg tccccgatgc
      121 aaacgcgccg agaacgacac ccggccggca acccagcaat gccaccgtca cctgagcccc
      181 cagggagagt cccgcgacaa aggcctcgcc tccatgcgct cgttctgcaa tgaggtcagc
      241 gacaagccgg gctgtgccgg caatcgagaa gagcccggcg tcatggctct gtccctgttc
      301 cggcatgtcg ggcacgagga cgtggacgtc aggcagtgcc gctatcgtat ctcgccacat
      361 ccagccgcca acacccgcgc cgtgcagcag gacaatagtt ggcgctcctg ccggtcccac
      421 ttcctgaaca tacattccca catcctgcct ccgtgccggg atccagcctg acacgcactc
      481 ggcacatgct cagtgtaagc ctgacgagaa ggtgcgcaag gagcgccgga ccagctggtt
      541 tggggagaaa cgacgagggg agaaggccga agtcttctcc cctgattcga acgacggagt
      601 gacgcgtcct acccgatgac ggccttctcg gtcagcagct cgccacgggc gaagcggcgg
      661 aagttgtagt gctcgatgtc gatgctgggc ttgccgtcag caatatactc ggcaagcagc
      721 cggccgacga tggggcccat catgaaccca tggcctgaat agccgtttgc ctggaagaag
      781 cccgggacct cgtcgactgg accaagtacg ggatgcgcgt ccggagagac gacatagtag
      841 ccggcccact gccgcataag cttgacgcct gcgaggcgcg gcacgatctc ggtcgcttcg
      901 cgcgagaact tggtcaggaa ctcgaaggtc gaatcgatgt tcgtcgcacc cttctcgccg
      961 gtatctgtac cgccgacgat attgccgcga tatgtctgaa ggtagtacag tgaacccttg
     1021 accagcatgg gtcccaggaa ctgctccagg ggctcagtgg ccatgatctg gtgcttgtca
     1081 agacgatagg ggaggtcaac gcccagcatg gctgcgatgg gcttggaacc agggcctgca
     1141 gcgttgacga cgttcttcgc ttcgatgttg ccttgtgtcg tgtggactgt gaacgtatgc
     1201 tccggggtcg tttccagtcc gaagacgtcg gtcgtcgtga ggatgtccac cccaagacgg
     1261 cgggcagcct gcgcatagcc ggtgactgtg tcgaagggga acgccatgcc atccttgtcg
     1321 taccactgac cggcgaggaa cgaatcggtg ttgagcgaag gacatatttc cttcgcctcc
     1381 tggcggtcta cgatgcggct catgacacca tgcttgttct ggaacgcgac gttctgcttc
     1441 gccttctcca cgagttcagg agtgtcccac aagaagaggt atccgcatgc cttgaagtgg
     1501 atgtcgtatc ccagctcgtc cgacatgcgc gcccacatct cttctgcttc gcgcagcagc
     1561 gtgatctggt cgggtccggt gaactgagca cgaatgccgg aaccacacct gccggtcgaa
     1621 ccactcccga ggtagcattg atcgagaacg gccacgtccc tgtagcctcg cttggcaagt
     1681 tcgtaggcga tggcaagtcc ggaaatgccg ccaccgatga tgacgcaatc gtagctactt
     1741 ttcatcgtcg acctccttgc ccttgacgaa gaacccgagc gggacagggt cgacgggtgg
     1801 gcgtgtggtg ggagtcatga tctgatccag ggtacgtcca gtctcgcgtg ccaggatctg
     1861 tgccgcgagc aggatacaag tcttgccctg gcaggggccg aggccgaact tggcatacct
     1921 cttgagtgac tgcaagtcag cgtagccggc atgaatggca tccttgatct cctggaccga
     1981 gatgtcctca cagcggcagg caatcgtctt ctcgtcttct ttggtcattg cgtcacccca
     2041 ccctgacgtt gcgcacctgc tcacagagag cagggtcgtc gaactcgaca gatatcagag
     2101 acgacctgct gacgctgtct gcaggcagca cgaacttcac cacggcactg ccgagcgaga
     2161 caccgtcgcg ccccagcagc gtcactgtgc tacctctgac cggcagtggc a
//
LOCUS       DATMZD010000147         3341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_144793,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000147 DATMZD010000000
VERSION     DATMZD010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144793"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1270
                     /gene="hflX"
                     /locus_tag="VN478_03090"
     CDS             <1..1270
                     /gene="hflX"
                     /locus_tag="VN478_03090"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HWQ21695.1"
                     /translation="EIPGASVMSGEGIARTAVIVSVRRIGEAVDPDRPEETRQLAEGL
                     GVEVLDSKTYVQRTPQRFLLGKGQVEELHELLQALRADLVIFDSELTPLQYRHMIDHL
                     ESSVMDRARLILETFASQAHTAEARKRVQIAERLFELAQLRAMNAGYDQQAGYIGMRG
                     PGETLFQKRRRAKRSEIHELREQVREVDTHYAIKTHTRQEAAIAKIAVVGYTNAGKST
                     LINLLAGSELLAEDKLFATLDTAARPAHFGRQSAVLIDTVGFIRDLPESLMDSFRSTL
                     EEAREADLLLHVVDAGVRDLNERIRVVDSVLGRIGCADIPRILIFNKMDTVDQARRQE
                     ILLIGTGFTISARTLEGVGQLNDQIAEALLRRVAHGRYVIPFTDPAARAAVYADAVVL
                     KERLAGRSWIIEAAGAAPGARLPERFLRKD"
     gene            1431..2453
                     /gene="queA"
                     /locus_tag="VN478_03095"
     CDS             1431..2453
                     /gene="queA"
                     /locus_tag="VN478_03095"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002573602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HWQ21696.1"
                     /translation="MNIRDFDYELPQGLIAQTPLEQRDLARLMVVDRASGKIQHRVFR
                     DLLSYLRPHDLVVVNDTRVNEASFSCRKDGTDAGIDVLLTGRRSPTVFQALVRPLKRL
                     HIGETCTVTPDVSLRLLERNDDGDALLEFSRDPYTSEGFRAVARVQIPPYIKQFPRDP
                     GAYQTVYAREPGSVAAPTAGLHFTAELLGEVRGLGVDIKHVTLDVGLGTFQPVRVETI
                     EDHHMHTERFTLPEETAVAVNAARANRAAVMAIGTTVARTLESCINPDGTVRADNGET
                     GIFIYPPHEFHGFDHLVTNFHLPKSTLLMLVGAFMGMDLMWTAYREAIQERYRFFSFG
                     DAMLIL"
     gene            2470..>3341
                     /gene="tgt"
                     /locus_tag="VN478_03100"
     CDS             2470..>3341
                     /gene="tgt"
                     /locus_tag="VN478_03100"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HWQ21697.1"
                     /translation="MFEVTHQSTTGRERTAVLTTAHGTVQTPVFMPVGTQATVKGLTP
                     EQVAEIGFEIILSNTYHLYLQPGDDVIQEAGGLHRFMHWNRSILTDSGGFQAMSLSRI
                     SKKSDEGIEFQSFIDGSRHMFTPERVIAIQKSLNSDVVMPLDICTQYPSERSVAEKDL
                     ARTVSWFGRAKAAMGESHQLLFGITQGGFYGDLREESTERIMEMEPQGFAIGGLSVGE
                     ERQLTMDMLDHSLLHAPEDRPRYFMGLGDPIGILEIISRGVDMFDCVLPTRVARNQMV
                     FTRDGVVKISKKVY"
BASE COUNT          709 a          934 c         1035 g          663 t
ORIGIN      
        1 agagatacct ggtgcctcag tcatgagtgg agaagggatc gctcgaacgg cagtgatcgt
       61 cagcgtcaga aggatcggcg aggccgtcga ccctgatcga cccgaagaga cgcgacagct
      121 ggccgaaggc cttggagtgg aagtgctcga ttccaagacg tatgttcagc ggactcccca
      181 gcgcttcctg ctcgggaagg ggcaggtcga agagctgcat gaactgctgc aagcgctgag
      241 agccgatctc gtcatcttcg actccgagct cacgccgctg caatatcgac acatgatcga
      301 ccatctcgag agttccgtga tggacagggc caggctcatc ctcgagacct tcgcgtctca
      361 ggcacatacg gccgaggcca gaaagcgggt ccagatcgct gaacgtctct ttgagttggc
      421 gcagctgagg gcaatgaatg cagggtacga ccagcaggca ggctacatcg gcatgcgcgg
      481 gccgggtgag accctctttc agaagcgccg cagggcgaag agatccgaga tccacgagtt
      541 gcgcgaacag gtgagggaag ttgacacgca ctatgcgatc aagacccaca ctcggcagga
      601 ggcggcaatt gcgaagatcg ccgtcgtcgg gtatacgaat gcgggaaaga gcacgttgat
      661 caacctgctc gctgggtctg agctgctggc agaggacaag ctgttcgcca ccctcgatac
      721 ggcggcacga cctgctcact ttggccgcca gtcggccgtg ctcatcgaca ctgtgggctt
      781 cattcgggat cttccggaat cgctgatgga ttccttccgt tccacgctgg aagaagcccg
      841 tgaggcagat ctcctgttgc acgtcgttga cgccggggtc agagacctga acgagcgcat
      901 tcgtgtcgta gacagcgttc tcggacgtat tggctgcgcg gacatcccca gaatcctgat
      961 cttcaacaaa atggatactg ttgatcaggc caggcgacag gagatcctgc tcatcggaac
     1021 gggtttcacg atttctgcca ggactcttga gggtgtcgga cagctcaacg accagattgc
     1081 cgaggccctg ctccgtcggg tcgctcacgg gcgatacgtc attccgttca ccgatcccgc
     1141 agcccgggcc gcagtatatg ctgatgccgt cgttctgaag gagaggctcg ctgggcggag
     1201 ctggatcatc gaggccgcag gagcagctcc cggcgctcgc cttcccgagc ggtttctcag
     1261 aaaggattga cagcagccac ccgtcgtgtc gccggtggtc ctgtgaacgc ctgcaagcct
     1321 tcgctctatt ggcaacgacg ggtgtgtccg tataatacac tctgccaggc tgtagactga
     1381 cgtgttgacg ttggggcaca gcggcatgaa gacgaggagc tggtcaacac gtgaatattc
     1441 gggacttcga ctatgagctg ccacaggggc tgatcgcgca gactcccctc gaacaacgtg
     1501 acctcgcgcg cctcatggtc gtcgatcgag cgagtgggaa gatccagcat agagtgtttc
     1561 gggatctcct gtcgtacctt cggccgcacg atctcgtggt ggtcaacgac acgcgcgtca
     1621 atgaagctag tttcagctgc cgcaaggacg gaaccgatgc cggtatcgac gtccttctca
     1681 ccggccgccg ttcgccgaca gtattccagg cactggtcag gcctctgaaa cgactgcata
     1741 tcggcgaaac gtgcactgtc acgccagatg tgagcttgcg gctgttggag cgcaacgatg
     1801 acggcgatgc gctgctcgag ttctcccgcg atccgtatac gagcgaaggg ttccgggcag
     1861 ttgcacgagt tcagattcca ccctacatca agcagttccc ccgtgatccg ggggcctatc
     1921 agacagtcta tgcaagagaa ccgggatcag tcgctgcgcc gactgctggg ctgcacttta
     1981 cggcggagct gctgggtgag gtgagaggtc tgggcgtcga tatcaagcac gtcacactcg
     2041 atgtggggct gggaacgttt caaccggttc gcgtcgagac gatcgaggat caccatatgc
     2101 atactgagcg cttcacgctg cccgaggaga ccgccgtggc cgtgaatgcg gcgcgtgcga
     2161 accgtgcagc cgttatggct atcggcacaa cggttgcaag gacgctagaa tcatgcatca
     2221 acccggatgg aactgtcagg gcagacaatg gtgagaccgg catcttcatc tatccacctc
     2281 acgaattcca cggattcgat catctggtta cgaactttca tctgccgaag tcgacacttc
     2341 tgatgctcgt tggggcattc atggggatgg atctcatgtg gacggcgtat cgcgaggcaa
     2401 tccaggaacg ataccgcttc ttcagctttg gcgacgccat gctgatcctg tagggtggtg
     2461 gccgtggcga tgtttgaagt cacacaccag agcacaacgg gacgtgaacg cacggcggtc
     2521 ctgaccactg cacacggaac agtacagacg cctgtcttca tgcctgtcgg aacgcaggcg
     2581 acggtcaagg ggctgactcc ggaacaagta gcggagatcg ggtttgagat tattctctca
     2641 aacacctatc acttgtatct tcagccaggc gacgacgtca ttcaggaagc gggcggactc
     2701 caccgattca tgcactggaa ccgaagcata ctcacggaca gcggtggctt ccaggccatg
     2761 agtctgtccc ggatcagcaa gaagagtgac gaaggaatcg agttccagtc attcattgat
     2821 gggtcgaggc acatgtttac gcccgagcgc gtgattgcca ttcagaagag cctcaactcg
     2881 gacgtggtca tgcctctgga catctgcacg cagtatccgt cggagcgctc tgtggccgag
     2941 aaggacctgg cgcgtactgt ctcatggttc ggccgggcga aggcagccat gggagaatcg
     3001 caccaacttc tgttcgggat cacgcagggt ggtttctacg gcgatctcag ggaggagtcg
     3061 accgaacgga tcatggaaat ggagccccag ggattcgcca tcggcggtct cagcgtggga
     3121 gaagaacggc agttgaccat ggacatgctg gatcatagcc tgctgcatgc tcccgaggat
     3181 cgccccaggt acttcatggg gctgggcgat ccgatcggga tcctggagat catcagccga
     3241 ggggtcgaca tgttcgactg tgttctgccg acgagggtcg cccggaacca gatggtcttc
     3301 acccgtgacg gcgtagtcaa gatctccaag aaggtctatg a
//
LOCUS       DATMZD010000148         2054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_114572,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000148 DATMZD010000000
VERSION     DATMZD010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114572"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="VN478_03105"
     CDS             <1..876
                     /locus_tag="VN478_03105"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWQ21698.1"
                     /translation="TSSGERLMDLRRARSTRRSELLLLLAVVLWSTTFPIMKQSLGSI
                     TPALLVLIRFAIASAVVYVLFRKRIEFTWLNVLKGLAIGIPEYLGFMLQVTGLTMTTA
                     SKNAFITGLCVVVAPVLSFLFLREHRSWKVLLALVLAVDGLWIISFGFTLRIGAMGMG
                     DFLTLLSALAFGVQVLMIHLFSRRDSFYATTFFQLLAVAVCSLVYVLTTKQTLVVNAV
                     SWPSLLYLAIFTTVITLLLQNRFQHDVTVAQASVIYSLEPVFATILAVLFIGEQMGPA
                     ALIGGILMFTASAIS"
     gene            991..>2054
                     /locus_tag="VN478_03110"
     CDS             991..>2054
                     /locus_tag="VN478_03110"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWQ21699.1"
                     /translation="MHYELKRYWTTIRRLGPNMRLLLAGAALLGLGNGIFGTFFNLYL
                     KEGGLGEAFLGRLNSLGTIGSLALAIPAGILVTRLGSRKSLALSVALMAIFGALQANA
                     LVPALLVVLQIGISASNMLYGAAYNPEMMRSTDPADRQVAFSLAFTVGTIMSVVASIV
                     GGLLPRLFERSGATPVMGLRLTLFVGAAFILASTAVFIRLRERPAHGDERVGDDPGDS
                     RHEPEVTSSWKRFAFRYALVNVIIGFGAGLTIPYLNIYFTSRFNASTVVVGNLFAMSN
                     VVLTAGVLLAPWLVRRAGLMRTIAVTIGGSLVFLIIMAFTKNIVVAALAFWMRAGLMM
                     CSTPVTDKFCLELVPPDKRSV"
BASE COUNT          347 a          694 c          577 g          436 t
ORIGIN      
        1 accagcagcg gagaacgcct gatggacctt cgacgcgcgc ggtccacacg caggtccgaa
       61 ctgctcctgt tgctggccgt cgtcctctgg tcgacgacct tccccatcat gaagcagagt
      121 ctcggcagca tcacgccggc actcctggtc ctcatccgct tcgccatcgc ttcggccgtc
      181 gtctacgttc tcttccggaa gaggatcgag ttcacgtggc tcaacgtgct gaaaggcctt
      241 gccatcggga tcccggagta cctcgggttc atgctgcagg tcaccggact cacaatgacg
      301 acggcaagca agaacgcatt catcacggga ctctgtgtcg tcgtcgcgcc ggtcctgtca
      361 ttcctgttcc tgcgcgagca ccgctcctgg aaggtcctgc tggctctcgt tctcgcagtc
      421 gacggactct ggatcatctc cttcgggttc acgctccgca tcggagccat gggcatgggc
      481 gacttcctga cattgctgtc ggcactcgcc ttcggcgtac aggtactcat gatccacctg
      541 ttctcccgca gggattcctt ctatgccacg accttcttcc agctcctggc cgtcgccgtc
      601 tgctcactcg tctacgtgct gacgacgaag cagaccctcg tcgtgaacgc tgtctcgtgg
      661 ccctccctcc tgtacctcgc catcttcact acggtcatca ccctcctgct gcagaaccgc
      721 ttccagcatg acgtgacagt cgcacaggca agcgtcatct actcgctcga gcctgtcttc
      781 gccacgatcc tcgccgtcct cttcatcggt gaacagatgg gccccgcagc gctcataggc
      841 ggcatcctca tgttcacggc gtcggcaatc tcatagcagg ccaggacaga accgcaccag
      901 gtgtgaaagc agactgcttg caggcgagcc tgcatgagct aaactgtttc ggaaattgaa
      961 ccatatcccg ccggccgaaa aggacacacg atgcattacg agctcaaacg atattggacc
     1021 acgatccgtc gacttggccc gaacatgcga ctgctgcttg ccggcgcggc attgctgggg
     1081 ctgggcaacg ggatcttcgg gacattcttc aacctgtatc tcaaggaggg agggctgggc
     1141 gaggcctttc tcggccgtct gaactccctc gggacgatcg gatcacttgc ccttgccatt
     1201 cctgcgggca tcctggtgac gcgactcggc agtcggaagt cgctcgccct gtcggtggcg
     1261 ctcatggcca tcttcggcgc actccaggcc aatgcactgg tgcccgcgct gctcgtcgtc
     1321 ctgcagatcg ggatctcggc cagcaacatg ctgtacggag cagcctacaa ccctgagatg
     1381 atgcgcagta cggacccggc cgaccgtcag gtcgcgttca gcctcgcgtt caccgtcggg
     1441 accatcatgt cggtcgtcgc gtcgatcgtt gggggactgc tgccgcgcct gttcgagcga
     1501 tccggtgcca caccggtcat ggggctccgc ctgaccttgt tcgtgggagc ggcgttcatc
     1561 ctggcgagca ccgccgtctt catccgactc cgtgaacgtc cggcccacgg cgacgagcgc
     1621 gtcggggacg accctggtga ctcccggcac gaaccggagg tcacatcctc gtggaagcgc
     1681 ttcgcattcc gctacgcgct ggtcaacgtc atcatcgggt ttggcgcagg gctgaccatc
     1741 ccatatctga acatctactt caccagccgc ttcaatgcaa gcactgtagt cgtgggcaac
     1801 ctgttcgcca tgagcaacgt cgtcctcacg gccggcgtcc tgctggcccc atggctggtg
     1861 cggcgcgcgg gcctgatgcg taccatcgcc gtcaccatcg gaggctcact cgtcttcctc
     1921 atcatcatgg cgttcacgaa gaatatcgtc gtggccgcac tggcattctg gatgcgcgca
     1981 ggtctcatga tgtgttccac gccggtcacg gacaagttct gcctggaact cgtgccaccc
     2041 gacaagcgtt ccgt
//
LOCUS       DATMZD010000149         2121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_74175,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000149 DATMZD010000000
VERSION     DATMZD010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74175"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1363)
                     /locus_tag="VN478_03115"
     CDS             complement(<1..1363)
                     /locus_tag="VN478_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HWQ21700.1"
                     /translation="MSGERKGFRHLSEGSYPATHVVGKSVDKVDGHALASGAPMYTDD
                     IAARDFPNRLHGKILTSPIANGAIESIDTTAAKALPGVRLVLTWKNTPRTFYTTAGQG
                     WPEPSPYDNQMFTERVRFVGDRVAVVFADSTEVAEQALKLIKVSFKEEPAVLNADDAM
                     KPGAPVIHPEDPKGTYDFAHNVAAHVDINVGDVEKALAESEVTISETFQTHYAQHTPM
                     ETHTAFAYTDDMGKLVIVSSTQVPWHTRRIVSRVLGLSMSKLRVIKPRIGGGFGTKQE
                     ILLDDLVAYGTIKTGLPCEIELTRKEEFVSARTRHPMKIDMSIGCDKQGHFKALKMYV
                     LSNTGAYGSHSLTVMSNTGSKTLPLYNKATDVRFFGDAVYTNLPVPGAYRGYGATQGY
                     AALETTIDDLAVKLGIDPIELRKMNMIRVGEGSPIFEALGEGREGVAQTIKSSSLPTL
                     LDLG"
     gene            complement(1360..1833)
                     /locus_tag="VN478_03120"
     CDS             complement(1360..1833)
                     /locus_tag="VN478_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HWQ21701.1"
                     /translation="MTISLKVNTIECSFDVQPDEVLLDVLRREGYLEVKGGCHTGDCG
                     ACTVLLDGRPVASCLMLAAKADGHAVTTVRGIGDQAHPHAIQDAFVRNGAVQCGYCIP
                     GAITSAKALLDENSNPTEEEVRLAMDSNYCRCSGYEQQVEAVLDAAAQLREGDAK"
     gene            complement(1830..>2121)
                     /locus_tag="VN478_03125"
     CDS             complement(1830..>2121)
                     /locus_tag="VN478_03125"
                     /inference="COORDINATES: protein motif:HMM:NF015415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21702.1"
                     /translation="DISTLHFSALVACDGKTVTGATLVFAGRPGHAALYPRLSEELVG
                     KPMSHGTFARLTEIAPDLVEVGTDLRATGDFRRELVRAAIVNLEEHMTGVAQ"
BASE COUNT          393 a          661 c          615 g          452 t
ORIGIN      
        1 cccccaaatc gagcagcgta ggcagcgagc tgctcttgat cgtctgggcg accccctcac
       61 ggccttcgcc gagagcctcg aagatgggtg agccctcacc gactctgatc atgttcatct
      121 tgcgcaactc gatggggtcg atgcccagct tgactgcgag gtcatcgatc gtcgtttcga
      181 gcgcagcgta tccctgggtc gctccatagc cacgatacgc tcccgggacg ggaaggttcg
      241 tatagactgc atcgccaaag aaccgaacgt cagttgcctt gttgtagagc ggcagcgttt
      301 tgctgccggt gttggacatg acggtgaggc tgtgactgcc gtaggccccg gtgttactga
      361 ggacgtacat cttgagagcc ttgaagtgac cctgcttgtc acagccgatg ctcatgtcga
      421 tcttcatcgg gtgacgggtc ctggccgaga cgaactcctc cttgcgggtc agttcgatct
      481 cgcacgggag acccgtcttg atagttccat aggccacaag gtcgtccagg aggatctcct
      541 gtttggtgcc gaagccacca ccgatccggg gcttgatgac gcgcaacttg ctcatggaga
      601 ggcccagaac tcttgagacg atgcgacgtg tgtgccaggg aacctgcgtg ctcgacacga
      661 tgacgagctt ccccatgtca tccgtgtacg cgaatgctgt atgcgtctcc atcggagtat
      721 gctgggcgta gtgggtctgg aacgtctccg agatcgtcac ctcggactcc gccaatgcct
      781 tctcgacgtc tccaacattg atgtcgacat gcgctgccac attgtgggca aagtcatagg
      841 tacccttggg gtcctcagga tggatgaccg gagctccagg cttcatcgca tcgtcggcgt
      901 tgagaaccgc aggctcctcc ttgaagctca ccttgatgag cttaagcgcc tgttctgcca
      961 cttctgtgga gtccgcaaag acaactgcca cgcggtcacc cacgaaacgc acccgctctg
     1021 tgaacatctg gttatcgtag ggagaaggct cgggccagcc ctggccggcc gtcgtgtaga
     1081 acgtacgcgg cgtgttcttc cacgtgagca cgagcctgac tccaggaagt gcttttgcgg
     1141 ccgtcgtgtc gatcgactca atggcaccat tcgcgatcgg actggtcagg atcttcccat
     1201 gaaggcggtt cgggaaatcg cgtgccgcga tatcgtccgt gtacatcgga gccccgctcg
     1261 ccagcgcatg gccatcgacc ttgtccacgg acttgccgac cacgtgcgtc gccgggtagg
     1321 acccctctga caagtggcgg aacccctttc tctctccgct catttcgcat ccccttcccg
     1381 gagctgtgcc gcagcatcaa ggacagcctc gacttgctgt tcgtacccgg agcatcgaca
     1441 gtaattgctg tccattgcca gccggacctc ttcttcggtg gggttggagt tttcgtccag
     1501 caatgccttg gcagacgtga tagctcctgg aatgcagtag ccacactgaa ctgcaccatt
     1561 ccgtacgaac gcgtcctgga tggcatgggg gtgagcctga tcgcctatgc cacggacggt
     1621 cgtcaccgca tgtccatcgg ctttggcggc cagcatcagg catgaggcca cggggcgccc
     1681 gtccagcagg accgtgcacg cgccacagtc tcccgtgtga cagcccccct tcacctcaag
     1741 gtagccttcg cgccgcagga catcgagcag gacctcatcc ggctggacgt cgaacgaaca
     1801 ttcaatcgtg ttcactttca acgagatcgt cattgtgcca cccctgtcat gtgctcttcc
     1861 aggttcacga tagccgcccg caccagttca cgcctgaagt cgcccgttgc acggagatca
     1921 gtgccaacct cgacgaggtc gggagcgatc tccgtcagtc tggcgaatgt cccatgcgac
     1981 attggcttgc cgacaagttc ctcggagagc ctggggtaca gagccgcgtg tcccgggcga
     2041 ccggcgaaca ccagggttgc tcccgtcact gtcttgccgt cgcacgcaac aagcgccgag
     2101 aagtgcagcg tggagatatc c
//
LOCUS       DATMZD010000150         2374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_14345,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000150 DATMZD010000000
VERSION     DATMZD010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14345"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="VN478_03130"
     CDS             complement(<1..506)
                     /locus_tag="VN478_03130"
                     /inference="COORDINATES: protein motif:HMM:NF017885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HWQ21703.1"
                     /translation="MAPRTRLSPRLMAYARQVRTRFPRVVAGTIVTDLDALADDACTS
                     LDDFPLTPGVLGCTVFEHGEAYTAIREGLRRDEPARYRFTLAHELSEILLRRYLDALP
                     DQCERERICDAFAAELLLPHDTVLASVTILVQAHAGGPGPSDATLDQLAQRLAFTYDV
                     SITAARISV"
     gene            complement(496..828)
                     /locus_tag="VN478_03135"
     CDS             complement(496..828)
                     /locus_tag="VN478_03135"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWQ21704.1"
                     /translation="MGFRDNVQTLLAHRGWSQRELAAKAGISPGAVGNILDGESVNLR
                     TAGKVAAAFIVEVADLVREEKPMEPGDELDVALRHQGDLSQGDILAVHAFISYIRGGR
                     STKGRRGT"
     gene            1063..1395
                     /locus_tag="VN478_03140"
     CDS             1063..1395
                     /locus_tag="VN478_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21705.1"
                     /translation="MKRNDAWFLPLYDGLRPAITGMARNYAQTWGLDTDDLVQVAGYA
                     LWSCCGRYRDRPLDEQLRIGNTVARRTMLHWCDHEGHVPTHLGTAGTSPWFTAGAGEG
                     LVAPQEES"
     gene            1392..1712
                     /locus_tag="VN478_03145"
     CDS             1392..1712
                     /locus_tag="VN478_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21706.1"
                     /translation="MSGLTLQRTSGDIAARVTLGLSMIDSRKAALIRDRFWRERTWPV
                     IAKEHHCSVTTAVKRFKQGMDDLRKAIMLVEGELPEATDCLPDDRPATVRSAQEAVNP
                     IGPC"
     gene            2060..>2374
                     /locus_tag="VN478_03150"
     CDS             2060..>2374
                     /locus_tag="VN478_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21707.1"
                     /translation="MRLTRKLLAIIIVLSMVLALAPKTASAAGFRGLTEADARQAQSD
                     YLNSLGSTSGSNAHAPGLVVDGFQIPSDVAAMKSFQNGYVRVMVTVPGDTISTYAVKL
                     SSS"
BASE COUNT          473 a          694 c          762 g          445 t
ORIGIN      
        1 accgaaatgc gcgccgccgt gatcgagacg tcgtaggtga aggccaggcg ctgtgccagc
       61 tggtcgagcg tggcgtcaga tggtcctgga ccgccggcat gagcctggac gagaatcgtg
      121 acactggcaa ggacggtatc atgtggcagc agaagctcgg ccgcgaaggc gtcgcagata
      181 cgttcgcgct cgcactggtc aggcagggcg tcaagatagc gtcgcaggag gatctcgctg
      241 agctcatgcg ccagcgtgaa gcggtagcgc gccggttcgt cgcgtcgcag gccctcgcgg
      301 atggcggtgt aggcctctcc atgctcgaag accgtgcagc ccaggactcc cggcgtcagc
      361 gggaagtcat cgagcgacgt gcaggcgtcg tcagcaaggg cgtccaggtc tgtgacgatt
      421 gtcccggcga cgacccgggg aaagcgcgtg cgcacttgcc gggcatacgc catcaggcga
      481 ggcgaaagcc gtgtcctagg tgccacgtct accctttgtc gagcggccgc cccggatgta
      541 ggagatgaag gcgtgcacgg ccaggatatc cccctgcgac aggtcgccct ggtgacgaag
      601 cgccacgtcc agctcatccc cgggctccat gggcttctcc tcccgcacaa ggtcggccac
      661 ttcgactata aatgcagctg ccaccttgcc ggccgtgcgc aggttgacgg actccccgtc
      721 gaggatgttg ccgacggccc ccggcgagat gcccgctttg gctgccagct cccgctgact
      781 ccagccacga tgtgcaagca gcgtttgaac gttgtctctg aatcccatcg tctgctcctt
      841 tcgcgtcggg tgaacaggcg cgcatgcttc gatccccaca agcgagatcc cgtgccggga
      901 cttgcgccgc gtgacacggc atcagaactg ttttctgccg tgtcctcctg atggtgtcta
      961 ccatattata cacgacagtg tagaatgtac acatcaagga atgttgttaa tagttgcgtt
     1021 gctcatgaaa cggccaaaag aatgcacacc tgggagggta gcatgaagcg aaacgatgcc
     1081 tggttcctgc cattatatga cggtttgcgt cctgccatca ccggaatggc ccgcaattac
     1141 gcgcagacgt gggggcttga tacggacgat ctggtccagg tcgccggcta tgccctctgg
     1201 tcctgctgcg gtcggtaccg ggaccgcccg ctggacgaac agttgcgcat cggcaacacc
     1261 gtggcacggc gtaccatgct gcactggtgt gaccacgagg ggcatgtgcc cacgcacctt
     1321 ggcactgcgg ggacgtcgcc gtggttcacc gctggcgcgg gggaggggct tgtggcgcct
     1381 caggaggagt catgagcggt ctcacactgc agcggacaag cggagacatc gcggcgcgtg
     1441 tcacactggg tctcagcatg atcgacagca ggaaggctgc cctcattcgt gaccgcttct
     1501 ggcgtgaacg cacgtggccg gttatcgcga aggaacatca ctgctctgtg acgacggccg
     1561 tcaagcgctt caagcagggc atggacgacc tcaggaaggc catcatgctg gtggagggag
     1621 aattgcccga ggccacagac tgcctgcctg atgatcggcc cgcaacagtc aggtccgctc
     1681 aggaagcggt gaaccccatc ggtccatgct gagaagacac gatacgatcc gggtcgaggc
     1741 ggagactacc ccaaattcgg cgtgagcaga gccagaactg ggaaggtgcg gggcggtcga
     1801 accagccggg tttggggtgt tcacctgttg ttgacttttc ggagcgtgcg ctatatgctg
     1861 tccaaatgag acgaaagccg tgcgtctttg cagtgaacgg cgccttgcag gctggacgtg
     1921 aggatcagga agcacggtcg agaggcggac aaggacactt caggactgct caatgcgaaa
     1981 ctggcgctac cgtgacaggt caaacgggag aacaaggtcg tatcgaattg aaggcacaca
     2041 caactggaga gggggatcaa tgagactcac ccgcaaactg ctcgccatca ttattgtatt
     2101 gagcatggtt ctggcacttg caccaaagac tgccagtgct gctggcttca ggggactgac
     2161 cgaagctgac gctcggcagg cccagtctga ctatttgaac tcgcttggat ccacttctgg
     2221 gagcaacgca cacgcaccag gtcttgtggt tgacgggttc cagatcccgt ccgacgtcgc
     2281 agcgatgaag tcgttccaga acggctacgt ccgcgtgatg gtcaccgtcc ctggagatac
     2341 tatctccacg tacgcggtga agctctcttc gtcc
//
LOCUS       DATMZD010000151         2737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_74189,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000151 DATMZD010000000
VERSION     DATMZD010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74189"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /gene="secG"
                     /locus_tag="VN478_03155"
     CDS             <1..233
                     /gene="secG"
                     /locus_tag="VN478_03155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HWQ21708.1"
                     /translation="FALLKTVLEIVMALVAVGDILAILFMEPKGSGLGAISGSATVFN
                     ARKPKDAVLDRLAVVLSIAFVVLSLVLVVFKF"
     gene            252..336
                     /locus_tag="VN478_03160"
     tRNA            252..336
                     /locus_tag="VN478_03160"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:286..288,aa:Leu,seq:aag)
     gene            358..432
                     /locus_tag="VN478_03165"
     tRNA            358..432
                     /locus_tag="VN478_03165"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:390..392,aa:Val,seq:tac)
     gene            509..584
                     /locus_tag="VN478_03170"
     tRNA            509..584
                     /locus_tag="VN478_03170"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:542..544,aa:Phe,seq:gaa)
     gene            complement(796..2181)
                     /gene="scfB"
                     /locus_tag="VN478_03175"
     CDS             complement(796..2181)
                     /gene="scfB"
                     /locus_tag="VN478_03175"
                     /inference="COORDINATES: protein motif:HMM:TIGR03974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HWQ21709.1"
                     /translation="MALSRIFPASVHLFERGGFFVLLDVPSGSIFSLDERVYRYLQLL
                     LADPASGDEAAQRLTGLDAETIVEIEDELEHLIAAGCLFTEATRPTEDASALTLKSLC
                     LNVAHECNFSCSYCFAHGGDYRGTATLMTRKVARAGIDFLVRESGSHHNVEVDFFGGE
                     PLMNWDVVVDTIDYARSTYASKKWRFTLTTNGSLLRDDMLPVLEANDVSVVLSLDGGR
                     KTNDANRVSKDGRGTFDTIVEKIRRFTRTRDEGGYFVRGTYARNTLSFAESVRELHSL
                     GFKYISMEPVVLSADSALALQTSDLPAIRVQYEELIAYFLSELGSGSGFDFFHFKLDL
                     EAGPCLSKRIYGCGAGVEYMAVAPNGDIFPCHQFDGIREYRMGNVLTTPALDHPGMVS
                     EFAHANFLFSKKECGDCWARFYCSGGCLANNFATTGSLLEPYALGCLIQKERIEAALA
                     VKAAESPENAV"
     gene            complement(2185..2352)
                     /gene="scfA"
                     /locus_tag="VN478_03180"
     CDS             complement(2185..2352)
                     /gene="scfA"
                     /locus_tag="VN478_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HWQ21710.1"
                     /translation="MKFIKVVATKTINVDDRLAKACRECQTPCKSACKTSSTVGNQVC
                     ETRKKPERFIW"
     gene            2430..>2737
                     /locus_tag="VN478_03185"
     CDS             2430..>2737
                     /locus_tag="VN478_03185"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HWQ21711.1"
                     /translation="MKTVYLVRHAETTWNLEGKVQGSQDTDLSTDGLAQTVKTVAFLS
                     TLQFDTILTSPLARARAIAEPVGVNLGILPIVMPELREIEFGSWEGHNWDEIGTMYPT
                     T"
BASE COUNT          618 a          776 c          763 g          580 t
ORIGIN      
        1 ggttcgcgtt gctaaaaacc gttttggaaa ttgtcatggc tcttgttgca gtcggtgaca
       61 tccttgcgat tctgttcatg gaaccgaagg gaagcggact tggcgcaatt tcgggcagtg
      121 caacagtgtt caacgcacgc aagcccaagg atgctgtcct tgacaggctt gcagttgtcc
      181 tatccatcgc atttgtggta ttgtccctgg tactggtagt cttcaagttc taggggagca
      241 acaagcctcg tgccggggtg gcggaattgg cagacgcgtg cgtttaaggg gcgcatggag
      301 caatccgtgt gggttcaatt cccaccctcg gcaccattca tgaataccac gatccagggg
      361 cggatagttc agcggtagaa cgcttgcctt acaagcaaga agtcgcaggt tcgaatcctg
      421 tttcgcccac cagcagtgtg atgtgtttgc ttgatggtgg gaccgctcat cgggtgaagt
      481 gcgatgagcg gtttctttga agtagagtgc cgagatagct cagttggtag agcaggggac
      541 tgaaaatccc cgtgtcgacg gttcaattcc gcctctcggc accattcttg ttcccagacc
      601 tgttctctca acccgcgaaa ccatcgtttt cgcgctcact gtcttcttcc accaggaaac
      661 ccagcagtcc acatgaccag cgtatacgtc cctcacgatg acgccagcga gtgcccgcct
      721 gacacaattg gcgccaggac gctgcacgca tcaggcggca acgggccatt ccccggactg
      781 cagatcagtg cagcgtcaca ctgcgttctc gggagactct gctgctttga cggcgagggc
      841 cgcctcgatg cgttccttct ggatcagaca gccgagagcg tatggctcca ggagactgcc
      901 agtcgtggca aagttgttgg caagacagcc accactgcag tagaagcgag cccagcagtc
      961 gccgcattcc tttttggaaa acaggaagtt ggcatgagcg aactctgaca ccattcctgg
     1021 atggtcgaga gcaggcgtcg tcagcacatt gcccattctg tactcccgga tgccgtcgaa
     1081 ctgatgacag ggaaagatgt ctccatttgg agcaactgcc atatactcga caccggcacc
     1141 acatccgtag atgcgcttgc tgagacatgg accggcttcc agatccagct tgaagtggaa
     1201 gaaatcgaac cctgagccag agcccagctc gctcaagaag tacgctatga gttcctcgta
     1261 ctgtacccga attgccggca gatcgcttgt ctggagggca agagccgaat ccgcagacag
     1321 cacgacaggc tccatggaga tgtacttgaa tccgaggctg tgcagctccc ggacactctc
     1381 cgcgaatgag agtgtgttgc gtgcgtacgt tccccgcacg aagtaacccc cttcatcccg
     1441 cgtgcgagtg aagcgccgta tcttctcgac gatagtgtcg aacgtgccgc ggccatcctt
     1501 cgacacacgg ttggcatcat tggtcttccg accaccgtcc aggctgagga caacggacac
     1561 gtcgttggcc tcaagcaccg ggagcatgtc gtcccggagt agtgagccgt tagtagtcag
     1621 cgtgaaccgc cacttcttcg acgcataggt cgaccgggca tagtcgatgg tgtcaacgac
     1681 gacatcccag ttcatgagtg gctcgccccc gaagaagtca acctcgacgt tgtgatgaga
     1741 accgctctct cgcacaagga agtcgatgcc ggcgcgcgca accttgcgcg tcatcagtgt
     1801 cgccgtacca cgatagtccc ccccgtgggc aaaacagtat gagcatgaga agttgcattc
     1861 gtgagcgacg ttcagacaga gggacttcag agtcaacgca gatgcatcct cggttggccg
     1921 tgtcgcctct gtgaacagac atcctgcagc tatgaggtgc tcaagctcgt cttcgatctc
     1981 aacgatcgtc tccgcatcaa ggccagtcag gcgttgggca gcttcatctc cgctggcagg
     2041 atcagccaga agcagctgga gataccggta gactcgctcg tcaagcgaga agatcgatcc
     2101 cgatgggacg tcaaggagca caaaaaaacc gcctctctca aacagatgga ccgaggcggg
     2161 gaagatacga gaaagcgcca cgagctacca gataaaccgt tcaggcttct tcctggtttc
     2221 gcagacctgg ttgcctacgg tcgaggacgt cttgcaagcg gacttgcagg gagtctgaca
     2281 ctcacgacaa gccttggcaa gcctgtcgtc gacgttgatg gtctttgttg ctaccacttt
     2341 gatgaacttc acagtgttgc tcccttctca aaccaggatt ttgtccacat aatatccacg
     2401 ataagcagcc ataagtcaag aggtacagca tgaaaaccgt gtatcttgta cgtcacgccg
     2461 aaacgacctg gaatctggag ggcaaggtcc aggggagtca ggacactgac ttgtccacgg
     2521 acggccttgc ccagacagtg aagacagtgg cgtttctctc aactctccag ttcgacacga
     2581 tcttgacaag tcccctggca cgcgcacgcg ccatcgctga acccgtcggc gtcaacctgg
     2641 gcattttgcc catcgtcatg cccgagctgc gagagatcga attcggaagc tgggagggcc
     2701 acaactggga tgagatagga accatgtacc ccacgac
//
LOCUS       DATMZD010000152         2357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_74190,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000152 DATMZD010000000
VERSION     DATMZD010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74190"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            338..2005
                     /locus_tag="VN478_03190"
     CDS             338..2005
                     /locus_tag="VN478_03190"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWQ21712.1"
                     /translation="MNPREQECADGSVERDDSRQATPATDPPDGPPGHDVSSPVPAAD
                     GRDSGQSAEKLRMILRCFEQSHDFIFALDTEGNLTMVNQTMCLTLGYSEQELLGKSVW
                     ELVDDASRHVIAEAVASVQRGTPVPLCTTPVISKSGQRLFMDISGQRLREGDQVIGSF
                     HIARDVSDRESVELEVQRTQALEALGNLAAGVAHDFNNVLTVIQGNTDLAETSVDQPE
                     TVQKHLAAVDSAVARARALAGQLLTFAHGGAPVKEDFDVSALIDDTAHFTLSGSNVAF
                     KSYVEPSLPQVNADRNQITQVIQSLVLNAREAMPSGGVVTVTARRQQIDGVRTLADAA
                     PGSYVVATIEDTGVGIKAADLGHVFDPYFTTKSIGHGLGLPIAHSIVRRHGGDMEVES
                     CPGVGTTVRFCLPVAAGSRPTDGARSQTIHATPVNGKGRILVMDDEQAVAEVVCAMAK
                     RLGYTAVAVPDGQTAIDVYALAAGTPAAFDVVVMDLTVPGGMGGKEAVTRLLDQFPDA
                     RVVVSSGYSDDAAMSDYSAYGFIGAIAKPYTIQELSEVLYPFSREGD"
     gene            complement(1978..2175)
                     /locus_tag="VN478_03195"
     CDS             complement(1978..2175)
                     /locus_tag="VN478_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21713.1"
                     /translation="MWKEEAAMNVSTLNNLDGDVWKMILENLYDAGDQEIQPPVPAGV
                     GFAAPEVNASEPGQSPSREKG"
BASE COUNT          498 a          760 c          710 g          389 t
ORIGIN      
        1 ggaagaagaa gtccaccagc gcgagtgagg tggccagacc cgtcttgtgg tatgcccaga
       61 tgctgagcgc aaacgccgtc atggccgagc cgatcatcga gaggacctgc cccgaccaca
      121 cgagcaggaa cgcacggaaa ccgctggggc gctgccattt cgggttcaac atcctgcgca
      181 gactcctctc tggcccgcgt cacgggtcca tggtgcaatg tcagactaaa agcatatgaa
      241 atcgttcctt cattgtcaaa cgagtccagc ataggctcct gggccgtcct tgtgccatat
      301 tccgggacgc gtatgatcat gtcacgaggg ggcaccaatg aatccacgtg aacaggaatg
      361 cgccgacggc agcgtcgagc gcgacgattc gcggcaggca actccagcga cagatccgcc
      421 ggacggtcct ccgggacacg acgtctcgtc gccagtgcca gccgcggacg gacgcgactc
      481 aggacagtcc gccgagaagc tgaggatgat actgaggtgt ttcgagcagt ctcacgactt
      541 catcttcgct ctcgacaccg agggcaatct caccatggtc aaccagacca tgtgcctgac
      601 gctgggatac agcgagcagg aactcctggg gaaaagcgtc tgggagcttg tggacgacgc
      661 aagcagacac gtcatcgccg aagcggtggc aagcgttcag aggggcacac cggtcccgct
      721 gtgcacgaca cccgtcatat caaagtctgg acagcggctg ttcatggaca tcagcggaca
      781 acgcctgcgg gagggcgacc aggtcatcgg gtcgttccat atcgcgcgag acgtctcaga
      841 ccgggagtcg gtcgagctcg aggtgcaacg cactcaggcc ctggaagcgc tgggcaacct
      901 tgcggcaggg gtcgcgcatg acttcaacaa cgtcctgacc gtcatccagg ggaacacaga
      961 ccttgcggag acatcggtcg accagcccga gaccgtgcag aagcacctgg cggcggtcga
     1021 ctccgctgtc gcacgagccc gtgcgctggc cggtcagctg ctgacgtttg ctcatggagg
     1081 cgctcccgtc aaggaggatt tcgacgtcag cgcactgatc gacgacaccg cacacttcac
     1141 gctcagcggc tccaatgtcg cgttcaagtc atacgtcgag ccctctctgc ctcaggtcaa
     1201 cgcagaccgg aaccagatca cacaggtcat ccagagtctc gtcctcaacg cacgggaagc
     1261 aatgccatca ggcggagtcg ttacggtgac agcccgcaga caacagatcg acggcgtgcg
     1321 gacccttgcg gacgctgccc ccggcagcta cgtcgtcgcc acgatcgaag acaccggagt
     1381 cggcatcaaa gcagcagacc tcggacacgt cttcgatccc tacttcacaa ccaagtccat
     1441 cggtcacggc ctgggactgc ccattgccca ttccatcgtg cgcaggcatg gcggcgacat
     1501 ggaggtggaa tcctgcccgg gcgtcggcac caccgtccgc ttctgccttc cggtggcagc
     1561 cgggtcccgt ccgactgacg gggcacgttc tcagacaata cacgcgacgc cggtgaacgg
     1621 caaaggcagg atcctggtca tggacgacga gcaggcggtc gccgaggtcg tctgcgctat
     1681 ggcaaagcgt ctcggataca ccgcggtcgc ggttccggac ggccagacag cgattgatgt
     1741 ctacgcgctg gcagccggca cgccggccgc gttcgacgtc gtcgtcatgg acctgacggt
     1801 accgggcggc atgggaggaa aggaagcggt caccaggctc ctggaccagt tcccggacgc
     1861 acgcgtcgtg gtctccagcg gatactcgga cgacgctgcc atgagcgact actcagccta
     1921 tgggttcatc ggcgccatcg ccaagcccta cacgatacag gaactgtccg aagtgctcta
     1981 tcccttctcc cgcgaaggag actgaccagg ttcggacgcg ttcacctctg gcgccgcgaa
     2041 gccgacacct gcagggacag gtggctggat ctcctgatcc cccgcgtcgt acaggttctc
     2101 caggatcatc ttccaaacgt cgccgtccag gttgttgagc gtcgagacgt tcatcgcggc
     2161 ttcttccttc cacacgagtg cgggtccacc cgcccgctta gcatacagcc gtgcggcatc
     2221 ctgtcaaaga ggagcacgac gaacacaatt cggacgccca gcgcgctata atccgtgaat
     2281 gccgaacacc gaagcgcaga aactatgaac gtcgaaccag cagcagtgca gcaacccgtc
     2341 caggcgatcc ccaggac
//
LOCUS       DATMZD010000153         2520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_84718,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000153 DATMZD010000000
VERSION     DATMZD010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84718"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VN478_03200"
     CDS             <1..207
                     /locus_tag="VN478_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HWQ21714.1"
                     /translation="DYVTTPVTPMGKLAFGVGAGLMTVLIRQLGNFPEGVTYGILLMN
                     VLTPYLDKIIPHKFGYVKPAKVNA"
     gene            204..1226
                     /locus_tag="VN478_03205"
     CDS             204..1226
                     /locus_tag="VN478_03205"
                     /inference="COORDINATES: protein motif:HMM:NF016121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HWQ21715.1"
                     /translation="MKRWQMIKLGLILGTYCIVGCVGLAFVYNSTSKTIAQRQQADLN
                     AGLAEVFPAATGFTPTDKVTSSNPKVVFDAAYEADGPSGSLGLAVKVHGPSYGGDTTL
                     IVGVSNDGTIAGVKVLENKDTPGLGANAANPKYYVDKATRTTFAGQFAGKKLTDGFEV
                     KKDVIAITSATITSRSITNLVQAVGKAASDALGIGAAPQGPGAVLPAADTVRASSKAA
                     TSSVPGVSILETSVGTKAGTIVGAAATAKAKSYDGEVTVLVGVSTDGTISGVQIVECT
                     DSLGGSILAPQFAQQFTGKKAGSPLTVGTNVDAVSGATFSSKGAAALVEATADALMKA
                     LVGGGL"
     gene            1223..1891
                     /locus_tag="VN478_03210"
     CDS             1223..1891
                     /locus_tag="VN478_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HWQ21716.1"
                     /translation="MKRLFGILANGLIVENPLFIIMIGLCSSMAVTTTVANGIGMGVA
                     LTFVLVMSELIIALFRNLIPDSIRMPIFLIVIAAFTTIMDLEMKAYFPALSKSMGVFI
                     PLVVVNCIVMGRVEAYSSKQPVLDSIFDGLGMGLGYTWVLIALAVVRELLGNGTLLGF
                     QIMPASFQPMIFFVLPPGGFLVFSLWMAFSLNVRRKMLAGRKPEPTQDSCCTLPDNGS
                     AEKA"
     gene            1900..2478
                     /locus_tag="VN478_03215"
     CDS             1900..2478
                     /locus_tag="VN478_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HWQ21717.1"
                     /translation="MTDLIKIFIAAFLINNIILMRFIALCSYIGMTSDIGQSVGMGLA
                     VTFVTVMASAVTWPIYYWILKPNGLEFLEILVFIVVIAALVQLVEFYLKKSVPTLYHA
                     MGIYLPLITTNCAILAVTFDQVTYSYNFVQSIVFALAVSLGYLLAMLLLAGVRQRLKN
                     SPIPAFMLETPVLYLATSIIAVAFMGFAGLIK"
BASE COUNT          496 a          790 c          708 g          526 t
ORIGIN      
        1 gactacgtca cgacaccggt cacgcccatg ggcaagctgg ccttcggcgt cggtgcagga
       61 ctcatgacag tcctgatccg acagctcggc aacttccctg agggtgtcac ctacgggatc
      121 cttctcatga acgtcctgac accatacctg gacaagatca tcccgcacaa gttcggatac
      181 gtgaagcccg cgaaggtgaa cgcatgaaac gctggcagat gatcaagctg ggactcatcc
      241 tggggacgta ctgcatcgtc ggttgcgtcg ggctggcctt cgtgtacaac agcacgagca
      301 agacgatcgc acagcgccag caggcagacc tcaatgctgg actggcggag gtgttccccg
      361 cggccacagg cttcaccccg acggacaagg taacgtcctc gaatcccaag gtcgtcttcg
      421 acgcggcata tgaggccgat ggcccgtctg gctcgctggg cctggccgtg aaggtccacg
      481 gccccagcta tggcggcgac acgacgctca tcgtcggcgt ctcgaacgac gggaccattg
      541 ccggcgtcaa ggtgcttgaa aacaaggaca cacctggtct tggggccaac gcggcaaatc
      601 ccaagtacta tgtcgacaag gcaaccagga cgaccttcgc cgggcagttc gccgggaaga
      661 agctcacgga cggcttcgag gtcaagaagg acgtcatcgc tatcacaagt gcaaccatca
      721 cgagtcgatc gatcactaac ctggtgcagg ccgtcggcaa ggccgcctct gacgctcttg
      781 gcatcggagc cgctccgcag ggacccggag cagtcctccc tgcagccgac acggttcggg
      841 cgtcctccaa ggctgcaacc tccagcgtgc ccggcgtctc catcctggag acctcggtcg
      901 gaaccaaggc cgggacgatc gtcggcgctg ctgcgacggc aaaggcaaag agctatgacg
      961 gcgaagtcac tgtgctcgtg ggtgtctcga ccgacggaac cataagcggt gtccagatcg
     1021 tcgaatgcac tgacagcctg ggcggttcca ttctcgcgcc ccaattcgcc cagcagttca
     1081 cgggcaagaa ggcaggttcc cccctgactg tcggcacaaa cgtcgacgcg gtctcagggg
     1141 ctacgttcag cagcaagggg gccgcagcgc tcgtggaagc gactgccgat gctctcatga
     1201 aggctctggt aggaggtggc ctgtgaagcg tctgtttggc atcttggcca acggtctcat
     1261 cgtagagaac ccgctgttta tcatcatgat tggcctctgc tcctcgatgg ctgtgacgac
     1321 cacggtcgcc aacggcatcg gcatgggcgt ggcgctcacc ttcgtcctgg tcatgtccga
     1381 actcatcatc gcactgttcc ggaacttgat cccggacagt atccgcatgc ccatcttcct
     1441 gatcgtcatt gccgcattca cgacgatcat ggaccttgag atgaaggcgt actttccggc
     1501 cctctccaag tccatgggcg tcttcatccc actcgtcgtc gtgaactgta ttgtcatggg
     1561 ccgtgtagaa gcatactcgt ccaaacagcc tgtcctggac tccatcttcg atggtcttgg
     1621 catgggactc ggctacacgt gggtgctcat cgccctggca gttgtccggg aactcttggg
     1681 gaacggtaca ctcctgggct tccagatcat gccggcatcg tttcaaccca tgatcttctt
     1741 tgtcctgcct ccgggtggat tcctcgtctt ctctctgtgg atggcgttca gcctcaacgt
     1801 ccgccggaag atgcttgcag gcaggaagcc ggaaccgacg caggacagct gctgcacgct
     1861 gcctgacaat ggctcggccg aaaaggcata ggagggaaga tgaccgacct gatcaagatc
     1921 tttatcgcag cgttcctcat caacaacatc atcctcatgc gcttcattgc gctctgctcg
     1981 tatatcggca tgacctccga catcggacag tctgtgggca tgggactagc cgtgaccttc
     2041 gtcacggtaa tggcttcagc agtcacgtgg cctatctact actggatcct caagccgaac
     2101 ggcctggaat tcctcgagat tctggtcttc atcgtcgtca tcgccgcgct agtccagctg
     2161 gtcgagttct acctcaagaa gagcgtccct acgctctacc acgccatggg gatctacttg
     2221 cccctcatca cgaccaactg cgctatcctg gccgtcacgt tcgaccaggt cacctacagc
     2281 tacaacttcg tccagtccat cgtctttgcg ctcgcggtgt cactgggcta tctgcttgcc
     2341 atgctgcttc tggcgggtgt gcgccaacgg ctcaagaact ccccgattcc cgcgttcatg
     2401 cttgaaacgc ctgtcctgta ccttgcgaca agcatcatag ccgtggcgtt catgggcttc
     2461 gccgggctta tcaagtgagg ctgcgatgaa gatcatcctg gaaacggcaa tgatctccgc
//
LOCUS       DATMZD010000154         3146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_74211,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000154 DATMZD010000000
VERSION     DATMZD010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74211"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1121)
                     /locus_tag="VN478_03220"
     CDS             complement(<1..1121)
                     /locus_tag="VN478_03220"
                     /inference="COORDINATES: protein motif:HMM:NF023495.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rnase Y domain-containing protein"
                     /protein_id="HWQ21718.1"
                     /translation="MPGTNGIISSAIYAGMLILGIVIGLLIEGYIARNRVESARELVR
                     KMEQEARSSIEAENAKNRLKLQEELEEKKNNLENKFVTRQTELQKTEHRLIQKETFLD
                     HRVEQVEQKDRDLDAAQEKLRGKTEILEGQRVAVETELQRIAQLNPEEARTIVLDRAE
                     KDTRSRVAKMVGEVVHDAQLQADAKAAEVIAASIQKIATDYVGESTISVVPLPSEDWK
                     GRIIGREGRNIRAFETLTGVDLLIDDTPEAVTLSAFNPIRREVARIALERLVVDGRIH
                     PAHIEQFVEEAEHQMDERVFGEGQQAANHVGIAGLSDEITRLIGRLKYRTSYGQNVLA
                     HSVEAAFIAANIASELKLPAGPAKRAGFLHDIGKAVDYEV"
     gene            complement(1198..2208)
                     /gene="recA"
                     /locus_tag="VN478_03225"
     CDS             complement(1198..2208)
                     /gene="recA"
                     /locus_tag="VN478_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003530638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HWQ21719.1"
                     /translation="MTDRDKALAAAMSQIEKQFGKGSIMKLGDHSDRPLVPAISTGSL
                     SLDAALGIGGLPRGRIVEIYGPEGGGKTTLALQAIAEAQKAGGRAAFIDAEHALDPVY
                     ARSIGVDVDELIISQPDYAEQALEIADMLAQSGGLDVFAIDSVAALVPRAELEGMMGD
                     QFVGLQARLMSQALRKLAGVLSKSQTCAIFINQLREKIGIAYGNPEVTPGGRALKFYS
                     SVRLEVRRVESITEGDAVIGNKVKVKVVKNKMAPPYRVAQFDIIFGKGIQTFGEVVDL
                     AVQFDIIEKSGSWFAYGGARLAQGRDNAAAYLKEHPDVMAEIKGKVREKLSPQVDPVP
                     AD"
     gene            complement(2314..2877)
                     /gene="thpR"
                     /locus_tag="VN478_03230"
     CDS             complement(2314..2877)
                     /gene="thpR"
                     /locus_tag="VN478_03230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HWQ21720.1"
                     /translation="MRAFVAIHLPDSFADWLEVSSARLGMLLSKVALVPAASCHVTLR
                     FLGELPGEQVDAAAERLRESISAVPGTTLVLDRFGVFRRGGRPAVLWIGPSVVSEALV
                     RLAASTDACMSGMGSNTRIEPFVPHVTLGRFVSGTRDGDAATIEAQCARPYAVPVTDV
                     VLYESLVGQGKPTYVARASVRLAPFAE"
     gene            complement(2874..>3146)
                     /gene="rimO"
                     /locus_tag="VN478_03235"
     CDS             complement(2874..>3146)
                     /gene="rimO"
                     /locus_tag="VN478_03235"
                     /inference="COORDINATES: protein motif:HMM:NF037386.4"
                     /note="catalyzes the methylthiolation of an aspartic acid
                     residue of the S12 protein of the 30S ribosomal subunit;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HWQ21721.1"
                     /translation="TKARRLRQFMEVQQGVSSSINQIRYLGRETDVIVDGASANEVRG
                     RTLIDAPDVDNTVVLHVGRRAQPARGDICRARITSVGPYDLYGILL"
BASE COUNT          619 a         1001 c          834 g          692 t
ORIGIN      
        1 acttcatagt cgaccgcctt cccgatatcg tgaaggaaac cagcccgttt tgccggcccg
       61 gcgggcagct tcaactcgga tgcgatgttg gccgcgatga aagcggcctc cacagagtgc
      121 gcaagaacgt tctggccata gctggtgcgg tacttgaggc gaccgatcaa gcgcgtgatc
      181 tcgtccgaga gaccggcaat gccgacatga ttcgccgcct gttggccctc gccaaaaacg
      241 cgctcatcca tctggtgctc tgcttcctcg acgaactgtt cgatgtgtgc ggggtggata
      301 cgaccatcga cgactagtcg ttccagagca attcgtgcca cttcccggcg aatcgggttg
      361 aacgcagaca gggtgacggc ctctggcgtg tcgtcgatga gtagatcaac gccagtcagg
      421 gtctcgaagg ccctgatgtt ccgaccctcc ctgcctatga tacgcccctt ccagtcttcc
      481 gaaggaagag ggacaacgct gatcgtagac tcacccacgt agtcggtggc gatcttctgg
      541 atggacgccg caatgacttc agccgctttg gcatcagcct gaagctgagc gtcatggacg
      601 acctcgccta ccatcttggc gactcgcgac cgcgtatcct tttcggctcg atccagaaca
      661 atcgtccgag cctcctcagg attcaactgt gcgatgcgct gcagctccgt ctccacagct
      721 actcgctgac cctcaaggat ctcggtcttg ccgcgaagct tctcttgtgc cgcgtcaaga
      781 tccctgtcct tctgctcaac ctgttcaacc cgatgatcca gaaaggtctc cttctgaatc
      841 aggcggtgct ccgtcttctg cagctcggtt tgccgagtga cgaacttgtt ctccaggttg
      901 ttcttcttct cctccagctc ctcttggagt ttgagcctgt tcttggcatt ctcggcctct
      961 atgctcgagc gcgcttcctg ttccatcttg cgcacgagct cacgcgccga ttcgacacgg
     1021 ttcctagcaa tatacccttc gataaggaga cctatgacaa tgccaagaat cagcatcccc
     1081 gcgtagatcg cagaggagat gatcccgttt gttccaggca tctggttacc atccttagta
     1141 tgtatgactc cggtccggca tgcgccgacc gaagtgcctt caaggaaccc tgccgaatca
     1201 gtctgcgggg actggatcca cttgaggcga aagcttctcg cggaccttgc cctttatctc
     1261 ggccataacg tctgggtgct ccttgaggta ggctgcagca ttgtcgcgtc cctgtgcaag
     1321 ccgtgcgccg ccgtaggcaa accacgaccc actcttctca atgatgtcaa actgcacggc
     1381 caggtcaacg acctcgccga acgtctgaat tcccttgcca aaaatgatat cgaactgtgc
     1441 cactcgatag ggtggtgcca tcttgttctt gacgactttc actttcacct tgtttccaat
     1501 gacggcatca ccttcagtga tgctctccac ccggcggact tccaacctta cggaggagta
     1561 gaacttcaga gctcgtccgc ctggtgtgac ttccggattg ccgtatgcga tgccgatctt
     1621 ctctcgcaac tggttgatga agatggcgca cgtctgcgac ttgctcaaga cacccgcaag
     1681 cttgcgcagg gcctgggaca tgagacgcgc ctgtagtccc acgaactggt cccccatcat
     1741 cccttccagt tcggctcgtg gtacgagtgc ggctacggag tcgatggcga acacgtccaa
     1801 accgcccgac tgggcaagca tgtcagcaat ctccagagct tgctcggcat agtcaggctg
     1861 cgagatgatc agttcgtcca cgtcgactcc gatgctacgc gcatacaccg gatcgagagc
     1921 gtgttctgcg tcgatgaacg ccgctctgcc gccagccttt tgcgcctcgg cgattgcctg
     1981 gagagcaaga gtggtcttgc ctcctccctc tgggccatag atctcgacga tcctgccgcg
     2041 tggaagccct ccaatgccga gggcagcatc aagcgagaga ctgcccgttg agatagcagg
     2101 aaccagcggc ctgtccgagt gatcgcccaa cttcatgatc gaaccctttc caaactgctt
     2161 ctctatctga gacatggcgg ctgccagagc cttgtcacgg tccgtcactc tgtcggctgc
     2221 ctcgttcttc tgtgccttgt cagtagtctt cccgttttcc atacagcctc cacatttggt
     2281 gtaagcccta cacctcagta taattcggtc tctctattcg gcaaatggag ccagccgcac
     2341 cgatgcgcgc gcaacatacg tcggcttgcc ctggcccacc aggctctcgt agagaacaac
     2401 gtcagtcact ggcacagcat atggccgtgc gcactgtgcc tcgatcgtcg cagcgtcgcc
     2461 gtcgcgtgta ccggacacaa aacgccccag cgtcacatgg ggcacaaaag gctcgatccg
     2521 ggtatttgac cccattccgc tcatgcaggc gtctgtcgat gcggccagac gaacaagagc
     2581 ctccgaaacg acacttggcc caatccagag aaccgcaggg cgcccgcctc ggcgaaacac
     2641 gccaaacctg tccagcacga gagtggtccc tggaaccgcc gagatggatt ccctcagacg
     2701 ttctgcagct gcgtcgactt gctcgcccgg aagctcaccc aggaatcgta gtgtcacgtg
     2761 acacgaggca gcgggcacca gtgccacctt tgagaggagc atgcccaacc tggcgctgct
     2821 gacctccagc cagtccgcaa aactatctgg cagatggatg gcgacaaatg ctctcaaagc
     2881 aggattccat aaaggtcata gggtccgaca ctcgtgatgc gtgccctgca gatgtcgccc
     2941 cgcgcgggtt gcgccctgcg cccgacatgc aggacgacag tgttgtctac gtccggtgca
     3001 tcgatcaagg tcctgccgcg gacctcgttc gcacttgctc catccacgat aacgtcagtc
     3061 tctctgccga gatagcgaat ctggttgatg gaagaggaga ctccctgctg gacctccatg
     3121 aactgacgca gccgccgggc cttcgt
//
LOCUS       DATMZD010000155         3776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_24026,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000155 DATMZD010000000
VERSION     DATMZD010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3776
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24026"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1352
                     /locus_tag="VN478_03240"
     CDS             <1..1352
                     /locus_tag="VN478_03240"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWQ21722.1"
                     /translation="GLLDRKFIERRSEGFEELVRSVETYTLELTSSVTGVPAALIQEA
                     ALAYGTSPASSILFGMGLTQHVGSTATITALCQLAVVTGNIGRPGTGVNPVRGQNNVQ
                     GTGDMGAVPDGLPGGPMSSSSVCAVLEQEWHVPLPQRRGLFATEVWDAAQSGKVRAAY
                     IIGENPVLSEADGASVIRGLEAMEFVVVQDIFMTETARYADLVLPASLWAEKDGTFTN
                     TERRVQRVRKVVESPEAANRDWRILIDMANALGADWYYRKPEDIFEEIRRVVPAYAGI
                     TYPLLNAPGFGLQWPVEEGGHETEILYTDRFATPSGKAHLCALELMNPVSGLTAEFPF
                     TLITGRLMAQYNTGTMSRRIDQLNRIAPAGYVELNREDALQMGVVPGEQVRVTSQSGS
                     LLTPAKVSSVLPRGFVFIPNHFRDTMLNVLVPNNTDPVAHIPAYKGVPVRIEKEEQQ"
     gene            1349..1603
                     /locus_tag="VN478_03245"
     CDS             1349..1603
                     /locus_tag="VN478_03245"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HWQ21723.1"
                     /translation="MKSLYICMGSACHLKSAQSIVDVFRRKIQEHQLEDVVELKGSFC
                     LGMCAEAVPVKVDETIVHGITVENASDMFEKEVMPLLRVT"
     gene            1605..2117
                     /locus_tag="VN478_03250"
     CDS             1605..2117
                     /locus_tag="VN478_03250"
                     /inference="COORDINATES: protein motif:HMM:NF024818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HWQ21724.1"
                     /translation="MTPEEEAGSELWRELWAHDPNALVVVDADLHICIVNPSFCSMFM
                     VNPALAVGMDISTVLGDAADFRQVFSTGQAVEAQTRRFPTYGRTLREVCFPVKEHHVV
                     AAIFHDITREESQADELHKIREESAQRVNAVVEDQMRVVQDVSGLLGEALAKTRVSLH
                     QLIKVIEKED"
     gene            2117..3265
                     /locus_tag="VN478_03255"
     CDS             2117..3265
                     /locus_tag="VN478_03255"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HWQ21725.1"
                     /translation="MRSYVYHRSLCKTGEEVCGDSVKYTASDENVFFVLSDGLGSGIK
                     ANIFSNLTVGIMLTMAAADMPIGEIVRTVSRTLPIDPERGMAYSTFTVVNVQPSGLST
                     FIVYDNPDPIILRNGALFMPDYEEKVVQDKILHKAVVQLSQGDTIFLISDGVTYAGLG
                     MSLRFGWGWENAAAFVEETQRATQSVEKTVDMLIDKVDRLYGGRPGDDATVLGAIVRS
                     LRRTVVLTGPPMDPADDAQVVSEFLQTPGEKVVCGGTTTNIISRETGRVAEVDVSTAR
                     PDCPPMGSMPGADLVTEGVLTISKAIQLVRQANGDERNIPHDRNGATELARTMLGSDH
                     VKFIVGLRVDPLYQNPLLPLSISLRKYLIEELAGLLRTAGKSVDIDYH"
     gene            complement(3347..>3776)
                     /locus_tag="VN478_03260"
     CDS             complement(3347..>3776)
                     /locus_tag="VN478_03260"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWQ21726.1"
                     /translation="RQVMQHDIVTVGPDCPIELAAATMADHKIGALPVVGPADSRSLV
                     GIITETDIFRLFVEMLGSRAAGVRASLEIDNRKGALASLFNRIVEAGGGVTTVSSFPA
                     ALPRRIRIILKVADVDQDTMRRLLKPEETVIDLREIQPGR"
BASE COUNT          750 a         1126 c         1197 g          703 t
ORIGIN      
        1 ccggcctgct ggaccgcaag ttcatcgagc gacggagtga gggattcgag gaactggtga
       61 gatcggtgga gacgtacacc cttgaactga cgtcgtctgt cacaggtgtg cccgccgcgc
      121 ttatccagga ggcggccctc gcctacggga cgtcgcctgc cagttccatc ctgttcggta
      181 tgggactgac gcagcatgtc gggagtaccg cgaccatcac tgcgctgtgt cagttggcag
      241 tcgtgacagg caacattggg cgtcctggca ccggcgtgaa cccggtgcgt ggccagaaca
      301 acgtccaggg caccggcgac atgggagcgg ttccggacgg actgcccggg gggccgatgt
      361 ccagtagttc cgtttgtgca gtcctggagc aggaatggca cgtcccgctg cctcagcgtc
      421 gggggttgtt tgcgacagag gtgtgggacg cggcacagtc ggggaaggtg agagccgcgt
      481 acatcatcgg agagaacccg gtactctctg aagcggacgg agcctccgtc attcgggggc
      541 tggaggccat ggaattcgtc gtcgtgcagg atatcttcat gacggagacg gccagatatg
      601 ctgatctggt ccttcctgcg tcgctatggg ccgagaagga tggcaccttc accaacacgg
      661 agcggagagt ccagcgtgtt cgcaaggtcg tcgagagccc cgaggctgca aaccgtgact
      721 ggcggatcct catcgacatg gcgaacgcgc ttggggctga ctggtactac cgcaagcccg
      781 aagacatctt cgaggagatc cgccgagtcg tccccgccta cgcgggtatc acgtatcccc
      841 tgctcaacgc gcccgggttc ggactccagt ggccggtcga agaaggtggg catgagacgg
      901 agatcctgta cacggaccga ttcgcgacgc cgtcggggaa ggcgcatcta tgtgcgctgg
      961 agctgatgaa ccccgtcagc ggactcaccg ccgagttccc gttcacgctg atcacagggc
     1021 gactcatggc ccagtacaac acaggaacga tgtcccggcg catcgaccag ctgaaccgca
     1081 tcgccccggc cgggtatgtt gagctgaacc gcgaagatgc tctccagatg ggggtcgtac
     1141 ccggcgaaca ggtacgcgtc acgtcgcaga gtggttcgct gctgacgccg gcgaaggtca
     1201 gttcggtcct tccgcggggc ttcgtgttta tccccaacca ctttcgggat accatgctga
     1261 acgtgctcgt gcccaacaac actgacccgg tcgcccatat ccccgcctac aagggtgtgc
     1321 cggtccgcat cgaaaaggag gaacaacaat gaagtcactg tacatctgca tgggaagtgc
     1381 ctgccacctg aagtctgcac aatccatcgt cgacgtattc cgcaggaaga tccaggaaca
     1441 tcagctcgag gacgtcgtcg aactgaaggg ctccttctgt ctcgggatgt gtgctgaggc
     1501 ggtccccgtc aaggtcgacg agaccatcgt ccacggtatc actgtggaaa acgcgtccga
     1561 catgttcgag aaggaggtta tgccccttct ccgggtgacg tgagatgacc cccgaagagg
     1621 aagccggcag cgagctctgg cgcgagctct gggcgcatga ccccaacgcg ctggtggtcg
     1681 tagatgcgga tctccacatt tgtatcgtca acccttcctt ctgctcgatg tttatggtga
     1741 accctgccct ggcagtaggc atggacatca gcaccgtgct gggcgacgcg gcggactttc
     1801 gccaggtgtt ctcgacgggg caggcggtcg aagctcagac gcggcgcttt ccgacctatg
     1861 ggaggactct ccgcgaggta tgcttccccg tgaaagaaca ccatgtagtt gccgccatct
     1921 tccatgacat tacgcgggaa gagagccagg ctgacgagct gcacaagatc cgtgaggaat
     1981 ccgctcagcg cgtgaatgcc gtcgtcgagg accagatgcg tgtcgtacag gacgtctcgg
     2041 ggctgctggg cgaagctctt gccaagacac gtgtctctct gcaccagctc atcaaggtca
     2101 tcgagaaaga ggattgatgc gatcgtacgt ctaccatcgc agcctgtgca agacgggcga
     2161 ggaagtctgc ggggactcgg tcaagtacac tgcctctgac gagaacgtct tcttcgtcct
     2221 gtctgacggt cttggaagtg gcatcaaggc caacatcttc agcaacctca cggtcggcat
     2281 catgcttacg atggccgcag ccgacatgcc catcggcgag atcgtgcgca cggtgtcccg
     2341 cacgttgccc atcgacccgg aacgcggcat ggcctactcc acgttcaccg tcgtcaacgt
     2401 gcagccgagt gggctcagca cgttcatcgt gtatgacaac cctgacccga tcatcctgcg
     2461 aaacggggct ctcttcatgc cggactacga ggagaaggtc gtccaggaca agatcctgca
     2521 caaggcagtc gtacaactct cgcaaggcga cacgatcttc ctcatcagcg acggcgtaac
     2581 gtacgccggc ctgggcatga gccttcgctt tggctgggga tgggaaaacg cggccgcgtt
     2641 cgtcgaggag actcagcgtg cgacgcaatc ggtagagaaa actgtcgaca tgctcatcga
     2701 caaggtcgat cgactgtacg gaggacgtcc gggagacgat gccacggtcc tgggcgcgat
     2761 cgttcgcagc ctgcggcgga cagtcgtcct cactggaccc cccatggacc cggctgacga
     2821 tgcccaggta gtgagcgagt ttcttcagac gcctggggag aaggttgtct gtggaggaac
     2881 gaccacgaac atcatctccc gcgagacggg acgcgtcgcc gaagtcgacg tgagcaccgc
     2941 gagaccggac tgtccaccca tgggctcgat gcccggggcg gacctggtga ccgagggagt
     3001 actgacgatc tcgaaggcga tccagctggt gcggcaggca aacggcgacg agcgcaacat
     3061 cccgcatgac cgcaacgggg ccacggagct ggcgcgaacc atgcttggat ctgaccacgt
     3121 gaagttcatc gtgggacttc gggtcgatcc actctaccag aacccgctgc tgccactctc
     3181 tatctcgctt cggaagtacc tcatcgagga actggcagga ttgctccgta ccgcaggcaa
     3241 gtccgtcgac atcgactacc actagcacga atggtatcat aagccacgaa gttgaagggc
     3301 tggcaggatc cggcggccct tcagccctgt cgcggcggaa ttctccctag cggcctggct
     3361 ggatctcgcg gaggtcgatg accgtctcct cgggcttcag gagacgtcgc atcgtgtcct
     3421 ggtcgacgtc tgctaccttg aggatgatgc ggatgcgccg gggcagtgcc gctggaaagg
     3481 acgacacagt cgtgacgccg ccgcctgcct cgacgatccg gttgaacagt gacgccaggg
     3541 cacccttgcg gttgtcgatc tcaagagatg cccgtactcc ggccgcgcgt gaaccgagca
     3601 tctcgacgaa cagtctgaag atgtccgttt cggtgatgat ccccacgagg ctgcgtgagt
     3661 ctgcaggacc gacgacgggc agtgccccga tcttgtggtc ggccatggtc gcggcggcca
     3721 gttcaatggg gcagtcaggt ccgactgtaa ctatgtcatg ctgcatgacc tggcgg
//
LOCUS       DATMZD010000156         2466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_144890,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000156 DATMZD010000000
VERSION     DATMZD010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144890"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1090)
                     /locus_tag="VN478_03265"
     CDS             complement(<1..1090)
                     /locus_tag="VN478_03265"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HWQ21727.1"
                     /translation="MTATGKSETLWWKRGIIYHCYVRSFMDGNGDGIGDLPGLISRLD
                     VLRDNTPSSLGIEGLWLSPVYPSPNRDFGYDISDYRSIDPTYGTMDDFDRLVDAARSR
                     GIHLIMDLVVNHSSTEHDWFRQARTSRKNQFHDYYLWNDGIGGRPPNNWGSSFGGSAW
                     EWNEQTHEFYLHSFLKEQADLNWRNPRLRQEIHDIMRFWMDRGVDGFRLDVANYYVKD
                     DQLRDNSHEFLVAQPMPGNTSRTLLIPMSREVYDHTVDQPETHHALRGMRAVIDEKPG
                     RMMVGEITQRTLGQVLSYYGDGDNELNLVFNFFFFVRRFRATDFRTIIQRTMDLMPEG
                     AWPAWVLSNHDVVRAVSRYHLTLPMTRTM"
     gene            complement(1104..>2466)
                     /locus_tag="VN478_03270"
     CDS             complement(1104..>2466)
                     /locus_tag="VN478_03270"
                     /EC_number="2.4.1.266"
                     /inference="COORDINATES: protein motif:HMM:NF010496.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="HWQ21728.1"
                     /translation="SNAVLAGTVVRHLTEAGVVDRTSFVNVTHAGESAAVDDSVLDAY
                     GKVKAELPQARLAIEQAEDGVESAIEHCSLQYGLLVLGLPQHPRKHSVTKHLLAQVTA
                     GLLRATSVFVYSRGIEVEQLSATTRAIEPWILKNTFSRDEFSDLDRLLQAKRNGHVSI
                     SLILPALNEEKTVGHVINAFKRPLMDEQPLLDEIILMDSDSSDNTRAIATACGIPVYV
                     HQQVRPDLGAYAGKGEAMWKALFVATGDILVFVDTDLSNPDPSFVTGLVGALLLDERL
                     NFIKGFYRRPVRVVNEFVEVGGGRVTELTARPLLNLWYPELGGLFQPLAGTIGVRAAT
                     LRDLHFLTGYGVEIGHVIEYVRKYGIDGLAQSELGEIVHRNQPLEALSKMSFQVLEAF
                     FKLSQGLMAPGATDRMNGIMRQPFLSETGFTLYQALLGQEMRPPASSVPPADPAASAP
                     PAA"
BASE COUNT          462 a          699 c          795 g          510 t
ORIGIN      
        1 tcatagttct ggtcatgggg agcgtcagat ggtagcgtga tactgcgcgg acgacgtcgt
       61 ggttgctgag gacccatgcg ggccaggcgc cctcaggcat gagatccatc gttctctgga
      121 tgatggttcg gaagtctgtt gcccggaagc gtcggacgaa gaagaagaag ttgaacacca
      181 ggttcagctc attgtcgccg tcgccatagt acgagagcac ctgccccagt gtacgctgtg
      241 tgatctcgcc caccatcatg cgtccaggct tctcgtcgat gactgcgcgc atgccccgca
      301 gagcgtggtg cgtctcgggc tgatccacag tgtggtcgta gacctcgcgt gacatcggta
      361 tcagcagcgt tcttgacgtg ttgcccggca tgggttgtgc aacgaggaac tcatgggagt
      421 tgtcccggag ctggtcgtcc ttgacatagt agttggcgac gtcgagacgg aacccgtcta
      481 cgccacggtc catccagaag cgcatgatgt catgaatctc ctgccggagc cgggggttgc
      541 gccagttcag gtccgcctgt tccttgagga agctgtgcag gtagaactca tgggtctgct
      601 cgttccattc ccaggccgag cctccaaatg acgaacccca gttgttggga ggtctgccgc
      661 cgattccgtc gttccagagg tagtagtcat ggaactgatt cttccgggat gtgcgggcct
      721 gcctgaacca gtcgtgctcg gtcgaactgt gattgacgac gaggtccatg atgaggtgga
      781 ttccccgcga gcgggcagca tccacgagtc ggtcgaagtc gtccatcgtg ccgtatgtgg
      841 gatcgatcga gcgatagtcg ctgatgtcat agccgaagtc ccggttgggc gacgggtaga
      901 ctggcgacag ccataggccc tcgatgccaa gagatgaggg ggtgttgtcg cgcaggacgt
      961 ccagtctgct gatcagtccg ggaaggtctc cgatgccgtc gccgttcccg tccatgaagc
     1021 tgcggacata gcagtgatag atgatgccgc gtttccacca caaggtttcg ctcttcccgg
     1081 tcgcagtcat gagtgctcct tgctcacgca gccggtgggg ctgaagcggc aggatcggcg
     1141 ggcggcacgg aggacgccgg gggcctcatc tcctggccca gaagtgcctg gtagagagtg
     1201 aaccccgtct ctgagaggaa gggttgccgc atgatgccgt tcatccggtc ggtcgcgcca
     1261 ggggccatca ggccctgcga cagtttgaag aatgcttcga ggacctggaa tgacatcttg
     1321 gaaagcgctt caagcggctg gttgcgatgg acgatttcac caagctcgga ttgggccagc
     1381 ccatcgatgc cgtacttgcg cacgtactcg atgacatgcc cgatctcgac accatatccg
     1441 gtcaggaaat gcagatcacg cagggtggcg gcgcgtaccc cgatggtccc ggcaagaggc
     1501 tggaagagtc cgccgagttc cggataccac aggttgagca gagggcgcgc ggtgagttcc
     1561 gtcacgcggc cgccgcctac ttccacgaac tcgttgacga cacgcaccgg tcggcggtag
     1621 aaccccttga tgaagttgag gcgctcatcc agcagcagcg caccaacaag gccggtgacg
     1681 aacgacgggt ctggattgga gaggtcggtg tcgacgaaga cgagaatgtc tcccgttgcg
     1741 acgaacagcg ctttccacat cgcctcgccc ttgccggcat aggctccaag gtccgggcgc
     1801 acctgctggt ggacatatac agggataccg catgcagtcg ctatcgcgcg cgtgttgtcg
     1861 gaactgtcgc tgtccatgag gatgatctcg tccagcagcg gctgttcgtc catcaggggg
     1921 cgcttgaacg cgttgatgac atgtccgacg gtcttctctt cgttgagcgc cggcaggatg
     1981 agagagatcg acacgtgtcc atttcgtttg gcctgcagaa gccggtcgag atccgagaat
     2041 tcgtcccggg agaacgtgtt cttgaggatc cagggctcaa tggccctggt cgtggccgac
     2101 agctgttcga cctctattcc gcgtgagtag acgaacacgg atgtcgcgcg cagcagcccc
     2161 gccgtgacct gagccagcag gtgctttgtc accgagtgct tcctggggtg ctgtggaagt
     2221 ccaaggacga gcaggccgta ttgcagcgaa caatgctcga tggcggactc cacgccgtct
     2281 tctgcctgtt cgatagcaag acgggcctgc ggcagttcgg ctttcacctt gccgtatgca
     2341 tcgagaacgc tgtcgtcgac agctgccgat tcgcccgcat gggtgacgtt gacgaaactc
     2401 gtcctgtcga cgacccctgc ttctgtgaga tgccggacaa ctgtcccggc gagcacagcg
     2461 ttggag
//
LOCUS       DATMZD010000157         2283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_4528,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000157 DATMZD010000000
VERSION     DATMZD010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4528"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="VN478_03275"
     CDS             complement(<1..799)
                     /locus_tag="VN478_03275"
                     /inference="COORDINATES: protein motif:HMM:NF016328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HWQ21729.1"
                     /translation="MTTIGDHLYARSTHGDLLARATNGAFVTTAMWYLLSTKRVDAVL
                     GVEHDRERNLTHARFIDDPREMAELAGTVVTAPLNVVQTLRKYGGTGGVALAVMPCEE
                     KLLDVLIRKDELPVQLFRIGLVCGGLFNGGDLTREVKRLGKSLLDVERVIHGEGGYFI
                     SFRGEEQIHEFPERKTVRPACERCVTPEPVSSHFSCGYWGTGSDRGRTYVTVNTAEAA
                     ELVDAMVRDGALTVESVPDDARAMRRHLIDLKTDASWRYRRRQFESDL"
     gene            1065..1874
                     /locus_tag="VN478_03280"
     CDS             1065..1874
                     /locus_tag="VN478_03280"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWQ21730.1"
                     /translation="MKLQDKVVVITGSTRGIGRSIALTCAGEGASVVVSSRSAGAVQR
                     MVDELKAQSYAAAGMACDVTVPGALAELLKFAVRTFGKVDIWINNAGLSGGYRPLDEM
                     SPDDISRIVATNVTALATGCSIVIPWMRAHGGGIIINMSGRGGKGEPSPWTALYAATK
                     AAVTSLTRSLAAENKGGSISIHAVIPGMVATDFYKDIPTSPRCSSELDNLPLVLDAFG
                     VPLDVVGQGFVDVAAQEPGRVTGQVYNLLHGRRLSRGILKMMYARLTHTLK"
     gene            2050..>2283
                     /locus_tag="VN478_03285"
     CDS             2050..>2283
                     /locus_tag="VN478_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21731.1"
                     /translation="MRRIMTVMAFAGGLLGLGNYVWDGWTMAFAGRNLYVPSNVMSVL
                     FLVVSGSVAATMLLRDHGRMTDEERKEYRATLIL"
BASE COUNT          431 a          722 c          706 g          424 t
ORIGIN      
        1 ccagatcact ctcgaactgg cggcgccgat accgccatga cgcgtccgtc ttcaggtcaa
       61 tcaggtgccg ccgcatggcg cgggcgtcgt cggggacgga ctcaactgtc agggctccgt
      121 cacgcaccat cgcgtcgaca agctcggctg cctcggccgt gtttacggtc acgtaggtcc
      181 tgccacggtc gctcccggtc ccccagtagc cacaggagaa gtgcgacgac acgggctcgg
      241 gcgtcacaca acgctcacag gctggccgca cggtcttccg ctccgggaat tcgtggatct
      301 gttcctctcc ccggaacgag atgaagtatc ccccttcgcc gtggatgacg cgttcgacat
      361 ccagcaggga cttgccgaga cgcttcacct ccctggtgag gtcgcctccg ttgaagaggc
      421 cgccacacac gagtccgatg cgaaacagct ggacgggaag ctcgtccttg cggatgagga
      481 cgtccagcag cttctcctca cagggcatca cggccagagc cacgcccccg gtgccgccat
      541 acttgcgcag cgtctggacc acgttaaggg gagccgtaac gacggtgccg gccagttcgg
      601 ccatctctcg cgggtcatcg atgaacctgg catgggtaag gttgcgctcc cggtcgtgtt
      661 ccacgcccag tacggcatcc actcgcttcg tcgacagcag ataccacatc gccgtcgtga
      721 cgaacgctcc gttcgtggcg cgagccagca agtctccatg ggtactgcga gcgtacaggt
      781 gatcgccgat cgttgtcatt gtagccccct tgggccgtga cctgtctacg ccatgcggct
      841 ttccacgccg atcgccgcgt gccgggacag gcgtctcctg ccccgtgaat tctaccataa
      901 ctgcggtttc acgacaacga gaaaccgtga cgtcgccact gtgtgaacaa atgtgcgcgg
      961 cagccagtcc aatcaatgcg ggcgaggaag ccaggtgccc tcctggtctc ttcgcccctc
     1021 ttcaggttct cactttcgtg gatatcctgt ggcagaggtg ctgcatgaaa ctacaggaca
     1081 aggtcgtcgt cattacgggt tcgacgcgag gcattggcag gtcgatcgca ctaacctgtg
     1141 cgggcgaagg agcgtcggtg gtcgtttctt cgcgctccgc aggtgcagtc cagagaatgg
     1201 tcgatgagct caaggcccag agctatgcag ccgccggcat ggcgtgcgac gtcacggttc
     1261 cgggagcgct ggccgagttg ctgaagttcg ccgtgagaac attcggcaag gtcgacatat
     1321 ggatcaacaa cgccggtctc tcaggcggct atcggcccct ggacgagatg agccccgacg
     1381 acatctcgcg gatcgtcgca accaatgtga cggcactggc gacaggctgc agcatcgtca
     1441 taccgtggat gcgggcacat ggtggaggga tcatcatcaa tatgagcggc cgcggcggca
     1501 agggtgaacc cagtccctgg acggcgctgt acgcagccac caaagctgcg gtcacaagcc
     1561 tgacgcgaag cctcgccgcc gagaacaagg gcggaagcat ctccatccac gcggtcatcc
     1621 ccggcatggt cgccacggac ttctacaagg acatcccgac cagtccgaga tgctcgagcg
     1681 aactggacaa tctgccgttg gtcctcgacg cctttggcgt ccccctcgat gtggtgggac
     1741 agggattcgt cgacgtcgcg gcgcaggaac ccggcagagt gacaggtcag gtctacaacc
     1801 tgctccacgg cagacggctg tctcgaggca tcctgaagat gatgtacgcc cgcctgaccc
     1861 acacgctgaa gtgactcatt gcctgtcagc ttctccgcag gcagcggtcc gagccggccc
     1921 tgtttccgca gagacaacga acgaccggca gcagaagcct atgcgctgtg ttcgtacgtc
     1981 tcgcggcaga atcccgatat actgagaccg gccgcggtga gagccgacga ccgatgagga
     2041 gcgtgctgga tgagaaggat catgacggtc atggcttttg caggcggcct gctgggtctg
     2101 ggcaactacg tgtgggatgg gtggaccatg gcctttgccg gccgcaacct ctatgtcccc
     2161 agcaatgtca tgtcagtgct gtttctggtt gtctccggct ccgtcgccgc gaccatgctg
     2221 ctgcgcgacc atggccggat gaccgatgaa gaacggaagg agtacagggc gacgctcatc
     2281 ctc
//
LOCUS       DATMZD010000158         2298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_95132,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000158 DATMZD010000000
VERSION     DATMZD010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95132"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="VN478_03290"
     CDS             <1..431
                     /locus_tag="VN478_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21732.1"
                     /translation="SGLGLTNVSFLSAVSRNQLHGLLSFADVAYAGVGASPLYHYGVS
                     LNKLYEYMTAGVCVLFAGAEDVFNDLVSEAGAGISVAHAVPGDIADGIRKLYGLTQEE
                     RTELGRRGMDYCQRNFSYEVLGRRYEAALEWACSHRRNRR"
     gene            404..1414
                     /locus_tag="VN478_03295"
     CDS             404..1414
                     /locus_tag="VN478_03295"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HWQ21733.1"
                     /translation="MQSQEESTVSTLVTGSLSLILTSIAVPLVRWAALRWGFLDRPDG
                     YLKPQAGAVPYGGGIAVVVGTSCALLWVRPVLLAIPIAGALLVLVLGVVDDSVGVRPV
                     WRLVAGLVAGSTLLALWPVTLLQLPARLLMGTCCAILASAAMNAINMTDGSDGLSGTV
                     SLLAAAGLLVGWSLAGQGALANVAAATCGGIAGFLIFNWPPARIYLGDAGAYWIGFVL
                     TALCIPALSSWHAVIGVMCVMALLEIELLASVLRRLRGHHGLATGDRRHIYDVVHLRL
                     GQSATKIDVLYGLVGVISSGVGILVWRGETVILGLVWLAALSMLVVLLYRWSACRKVV
                     VR"
     gene            1411..>2298
                     /locus_tag="VN478_03300"
     CDS             1411..>2298
                     /locus_tag="VN478_03300"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HWQ21734.1"
                     /translation="MNVPLSSPWIDESDEQAVLRVLRSDHLSLGPRVPEFEDGLAHVA
                     HVAHGVAVNSGTSALHLIVRALGLGDGDEVITTPFSFIASSNCLLFERIRPVFVDIRD
                     DTYNIDTTRIEAALTSRTRGILAVDAFGQPAELDIIEDIAHRNHLRVIEDSCESLGSE
                     WKERRCGGWGDAATFGFYPNKQITTGEGGCIVTNSDEIDRLARSMRNQGRGETDDWLG
                     HERLGYNYRLPDINCALGSSQLARLDTIIERRARVAGWYSSRLARSRDVVVPWIAPEV
                     TRMSWFVYVIRLADSFSMSD"
BASE COUNT          398 a          650 c          758 g          492 t
ORIGIN      
        1 cttcggggct gggtctgacg aacgtctcgt ttctctctgc tgtttccagg aaccagctgc
       61 acggactgct ttcgtttgca gacgtagcct acgccggggt cggggcaagc ccgctgtatc
      121 actatggagt ctctctcaac aagttgtacg agtacatgac ggcgggcgtg tgtgtcctgt
      181 tcgcaggtgc tgaagatgtc ttcaatgacc tcgtgtcaga ggctggagcc gggatctcgg
      241 tagctcacgc tgttccaggg gacatcgccg acggcatccg caagctgtat gggctgacac
      301 aggaggagcg taccgaactc ggacgaagag gaatggacta ctgtcagcgg aacttctcct
      361 acgaagtact gggacggcgc tacgaggccg cgctggagtg ggcatgcagt cacaggagga
      421 atcgacggtg agtacgcttg tgacagggtc gctgtccttg atcctgacgt ctatcgccgt
      481 gccactcgtg agatgggctg cactgcgatg gggcttcctg gaccgaccgg atggctactt
      541 gaagccacag gccggagctg tgccctacgg cggtggcata gcggtcgtcg tcggcacaag
      601 ctgtgccctg ttgtgggtgc gaccagtgct actcgccatc ccgatcgcag gggctctgct
      661 ggtgctcgtg ctcggcgtgg tggatgactc ggtcggtgtg cgccccgtgt ggagacttgt
      721 cgcagggctc gtcgccggca gcacgctgct ggcactctgg cccgtcacac tcttgcagtt
      781 gccggctagg ctgctgatgg ggacgtgctg tgcgatcctg gcgagcgcag ccatgaacgc
      841 gatcaacatg accgacggca gcgacggctt gtccggtacg gtcagcctgc tcgcggcggc
      901 aggtcttctc gtcggctggt ctctcgccgg gcagggtgcc ctggcgaatg tcgccgctgc
      961 aacatgtggc ggcattgccg gcttcctcat cttcaactgg ccgcccgccc gcatatacct
     1021 cggagacgcg ggagcgtact ggatcggatt cgttctcacg gcgctgtgca ttcctgcact
     1081 gtcgtcgtgg catgctgtca tcggcgtcat gtgcgtcatg gctctgctcg aaatcgagtt
     1141 gctcgcctcc gtcctcaggc gcttgcgcgg gcatcatggt ctggcgaccg gcgaccggag
     1201 acacatctat gatgttgttc acctgagact cgggcagtct gcgacgaaga tagacgttct
     1261 gtatggtctt gtcggtgtga tcagttctgg tgttgggata ctggtgtggc ggggggaaac
     1321 tgtgatactg ggactggtgt ggttggcagc actatctatg cttgtcgttc tgctgtatcg
     1381 atggtcagca tgcagaaagg tggtggtgag atgaatgtac cgttgtcttc tccatggatt
     1441 gacgaaagtg acgagcaggc agtgctgcgt gtgctcagga gcgaccatct gagcctgggt
     1501 cctcgtgtgc cggaattcga ggacgggctg gcgcacgtcg cgcacgtcgc gcacggagtt
     1561 gccgtgaaca gcggcacgtc ggcgttgcac cttatcgtgc gcgcgctcgg cctcggggat
     1621 ggcgacgagg tcatcacgac gccgttcagt ttcattgcct catcaaactg tctcctcttc
     1681 gagcgcatcc gacccgtgtt cgtggacatc cgggatgaca cgtacaacat cgacacgacg
     1741 cgtatcgaag ctgctctcac gtccaggacc agggggatcc ttgcggtcga tgcctttggc
     1801 cagcccgcgg aactggacat catcgaggac atcgctcaca ggaaccacct gcgtgtgatc
     1861 gaggattcct gcgagtcgct cggatccgaa tggaaggagc gtcgctgtgg agggtgggga
     1921 gatgcagcga cattcggatt ctacccaaac aagcaaatca caaccggaga gggcggatgc
     1981 atcgtcacca actccgacga gatcgaccgg ctggcccgaa gcatgcgaaa ccagggacgt
     2041 ggcgagactg acgactggct cggacatgag cgtctgggat acaactaccg cttgcctgac
     2101 atcaactgcg cgctggggag ttcccaactg gcgcggctcg acacgatcat cgagcgcaga
     2161 gcacgggtgg ctggctggta ctccagccga ctcgcgcggt cccgggatgt cgtcgtccca
     2221 tggatcgctc ccgaggtcac ccgcatgagc tggttcgttt acgtgatccg gcttgcagac
     2281 tcgttctcga tgagcgac
//
LOCUS       DATMZD010000159         3136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_85180,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000159 DATMZD010000000
VERSION     DATMZD010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85180"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="VN478_03305"
     CDS             complement(<1..872)
                     /locus_tag="VN478_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21735.1"
                     /translation="MKKFLAIVLVIGLVVGVMGPIARVSAADQSTTTPPDGWSSSGVH
                     PVIETLLPSGVPVLVLEDAYPWGSTSHITLLASLGYAPHMATWSDLGVSVNLSDFKNV
                     YVASDQPQSFYDGYAAHAAELTAWVQAGGHLVFSACNQGFAGGSITTPLPGGFVQAHR
                     YATTNVVADSAHPIATGILSNGVPLASPLTGTYASHSYFSVLPELADVIIRDDSHGAT
                     LAEYPLGAGLVIASGLTWEIAYELGWTFATSAYDDLFLYAFPKTKHMIAATAGTGGSI
                     SPSGDVTVYEGSSRT"
     gene            complement(1016..>3136)
                     /gene="uvrA"
                     /locus_tag="VN478_03310"
     CDS             complement(1016..>3136)
                     /gene="uvrA"
                     /locus_tag="VN478_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HWQ21736.1"
                     /translation="DYLRLLFARAGVPYCPEHDIPITKQSPQEIVEHLLADYAGHTVA
                     LLAPAVEGRKGEYRKLIEDVQRKGFESYRVDGTGYSVDDPVPVIDKKRKHTIELIVDR
                     LLVTADERTRLFDSVELTLKEGNDVLKVLDLATGKIDFFSSTFACPICGYSIQEIEPR
                     LFSFNSPYGACPTCHGRRLKPEALAVKLAGLNITEVTDMSVDQASQFFGHLPETMSET
                     QRTIGKQVLREINERLKFILDVGLGYLTLSRASATLAGGEAQRLRLATQVGSKLVGVL
                     YVLDEPSIGLHPRDNERLLSTLRELRDLGNTLVIVEHDEETIRTADYLVDVGPGAGEH
                     GGHIVAAGSLADIIAEPTSLTGQYLTGKLSVPLPKTRRKPNGEHLLIKGATEHNLKNV
                     DADIPLHMFTCITGVSGSGKSTLINDCLFKGLARELYHAKDTPGHYERLTGVEYIDRV
                     IVVDQTAIGRTPRSNPATYTGLFTPIREVYARMPEAKMRGYQPGRFSFNVKGGRCEAC
                     EGNGFTKVEMQFLPDVYVPCEVCHGARFNSETLEVKYKGKSIADVLEMSVEEALHFFD
                     AHARLKHMLKLLDAVGLGYIRLGQSAVTLSGGEAQRIKLAAELGKRTAGRTLYILDEP
                     TTGLHFADVEKLVGVLQELTNKGNTVIVIEHNLEVIKNADWVIDLGPEGGDKGGRIIA
                     TGTPEQIARSPASLTGQFLKDKVR"
BASE COUNT          586 a          951 c          959 g          640 t
ORIGIN      
        1 gtcctggagg atccctcgta caccgtgacg tcaccagagg gcgagatgga gccgccggta
       61 ccggcggtgg cggcaatcat atgcttcgtc ttgggaaagg cgtagaggaa gaggtcgtca
      121 taggctgacg tcgcaaaggt ccaccccaat tcatacgcga tttcccacgt gaggccggat
      181 gctatcacga gacctgcacc gagtgggtac tcggccagag tggcgccgtg cgagtcgtca
      241 cggatgatga cgtccgcaag ttcgggcaga accgagaagt acgagtggct ggcataggtg
      301 ccagtgagtg gggacgcaag aggaacgccg ttggaaagaa tcccggtggc aatagggtgc
      361 gcggagtccg cgacaacgtt cgtggtcgcg tatctgtgtg cctgaacgaa tcctcccgga
      421 agcggagtag taatagagcc gccagcaaaa ccctggttac aggcggagaa caccaggtgc
      481 ccaccagcct gtacccatgc cgtcagttcg gcagcatgcg ccgcatagcc gtcatagaag
      541 gactggggct ggtcgctcgc aacatagacg ttcttgaagt cgctcaggtt cacactcacg
      601 cccagatcac tccacgtcgc catgtgggga gcgtacccga gcgacgccag aagggtaatg
      661 tgtgacgtgg agccccaggg atatgcgtcc tcgagaacca gaacaggaac tccactcggt
      721 aacagcgtct caatgaccgg atggactccg gatgacgacc aaccgtcagg gggagttgtc
      781 gtcgactggt cagcggcaga taccctggca atcggaccca tcacaccaac tacaaggcct
      841 attacgagaa caatagccaa gaacttcttc attcgaacct ccatgttgtt cgggcctgtc
      901 tcttcccgat gctccgcttg cttccacata ctgcaggtat ttgaagcgtt tctcggcttt
      961 tgtcaagccc ttcgccgcgg accgcgcaat tgggtctttc cgtccgtgcg gcctttcacc
     1021 gcaccttgtc cttgaggaac tgcccggtca gtgatgcagg gctccgggcg atctgctccg
     1081 gcgttcccgt cgcgatgata cggccgccct tgtcgccgcc ctcaggaccc aggtcaatga
     1141 cccagtcggc gttcttgatg acctccaggt tgtgctcgat gacgatgaca gtgttgcctt
     1201 tgttggtcag ctcctggaga acgcccacga gcttctccac gtcggcgaag tgcaggcccg
     1261 ttgtcggttc gtcgaggatg tacagcgtcc ttcccgctgt ccgcttgccg agctcggcag
     1321 ccagcttgat gcgctgggct tcgccaccag acaaggttac cgcactctgg cccagtcgga
     1381 tgtacccaag tccgactgcg tccagcagct tcagcatatg tttgagccgt gcatgtgcgt
     1441 cgaagaagtg cagggcctcc tcgactgaca tctccaggac gtctgcaatc gactttccct
     1501 tgtacttcac ctccaaagtc tcagagttga agcgcgcacc gtggcagacc tcgcacggaa
     1561 catagacgtc aggcaggaac tgcatctcga ccttggtgaa gccgttccct tcgcaggcct
     1621 cgcaccggcc tcctttcacg ttgaaactga agcgcccggg ctggtatccg cgcatcttcg
     1681 cttcaggcat gcgcgcgtag acctcgcgaa tcggcgtgaa caaccccgtg taggtggcag
     1741 ggttgctccg cggcgtgcgc ccgatggccg tctggtcgac aacgatgacg cggtcgatgt
     1801 actcgacgcc tgtcagtctc tcatagtgac cgggagtatc cttggcgtgg tacagctcgc
     1861 gggccagccc cttgaacagg cagtcgttga tgagtgtcga cttgcctgag ccggagacgc
     1921 cggtaatgca tgtgaacata tgcaggggga tgtctgcgtc gacattcttg aggttgtgct
     1981 cggtcgcgcc cttgatcaac aggtgttcac cgttgggctt gcgcctggtc ttgggcaggg
     2041 gaacggacaa cttgcccgtc aggtactgtc ccgtcagcga cgtcggctcg gcgatgatgt
     2101 ctgcaaggct ccctgcagcc acgatgtgtc cgccgtgctc gcccgcccct gggcccacgt
     2161 cgacaaggta gtcggcggtg cggatggtct cctcgtcgtg ctccacgatg accagcgtgt
     2221 tcccaagatc gcgtagttca cgcagtgtgc tcaggaggcg ctcgttgtcg cgcggatgca
     2281 ggccgatgga cggctcgtca agcacgtaca ggacccccac cagcttgctt cccacctggg
     2341 tcgccagcct caggcgctgc gcttcgccgc cggcgagcgt cgccgacgct cgcgaaagcg
     2401 tcaggtatcc caggccgacg tcgaggatga acttcagacg ctcgttgatc tcgcgcagaa
     2461 cctgcttgcc gatggtgcgc tgggtctcgg acatggtctc gggcagatgc ccaaagaact
     2521 gcgaggcctg gtccaccgac atgtccgtca cttcggtgat attgaggccg gccagcttca
     2581 ccgcgagtgc ttcgggtttc aggcgcctcc cgtgacacgt cgggcatgcg ccatagggag
     2641 agttgaacga gaagagacgc ggctcgatct cctggatgct gtagccgcag atgggacagg
     2701 caaacgtgct cgagaagaag tcgatcttcc ccgtcgcgag gtccaggacc ttgagcacat
     2761 cgttcccttc cttgagggtc agctccaccg aatcgaacag acgcgtccgt tcgtccgcag
     2821 tgacgagaag cctgtccacg atgagttcga tcgtgtgctt gcgcttcttg tcgatgactg
     2881 gtaccgggtc gtcgacactg taccctgtgc cgtccacgcg atagctctcg aatcccttgc
     2941 gctgaacgtc ttcaatgagc ttgcggtatt cccctttgcg cccctcgact gcaggcgcga
     3001 gcaaggcgac ggtgtgcccc gcatagtcag ccagcaggtg ttcgacgatc tcctgaggcg
     3061 actgcttcgt aatagggatg tcgtgctcgg ggcagtatgg gacgccggcg cgcgcgaaca
     3121 gcagacgcag gtagtc
//
LOCUS       DATMZD010000160         2530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_55117,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000160 DATMZD010000000
VERSION     DATMZD010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55117"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..1098)
                     /locus_tag="VN478_03315"
     CDS             complement(160..1098)
                     /locus_tag="VN478_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21737.1"
                     /translation="MTTKTVFYSVTGHSRTVAEGAAKTLDSPLFGLTDRFSALPPGWK
                     RASRQAVRSFDLGVLSTPDVSFGRGDRLVLVFPYWNGAIVPAVSGFVRSVELTGVTVF
                     LVMTRRWSGGDELVAQLEDDVVRQGGTIGDVFSLRTFWRGPRVLRSLGNRIGRRIEAQ
                     EQGVATSLLELLENAIDDEVEARSRYVQLVEMTPDRRLKARFSSLAADKAAHTQQLQQ
                     LYRAYAGILYEPGREPIVSLTAGQVLDYGALLQGLDTVVEAEHKAFRGYWAIAERYPS
                     QPDVVRQMLSLSRSDLRHYRDMRGTYAALSRHHSKR"
     gene            complement(1178..2122)
                     /locus_tag="VN478_03320"
     CDS             complement(1178..2122)
                     /locus_tag="VN478_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013681599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HWQ21738.1"
                     /translation="MPTLQEIALKTAGADARIVSGHRMCSGCGIASIVKTVLAASDKP
                     VIVLNATGCLEVTTAVYPFTAWNTPWMHLTFENAAAVAAGVESAYKALKRRGKIDCDV
                     NILAVGGDGGTYDIGFQALSGALERRHKFMYLVYDNEGYMNTGNQRSGATPFGASTST
                     VPAGKVSFGKPQWRKNLVEIAAAHRIPYVGQAAVHATLDLFKKAQKGFNADGPAVLAA
                     FQPCTTNWSYDPALTMQYSKLAVETNFWPLYEIENGVVHITTKPSNRKPVEEFLKGQS
                     RFKHLFRPGNEHIIVEMQTMIDEEWARLLKLEESGIQF"
     gene            complement(2128..>2530)
                     /gene="porA"
                     /locus_tag="VN478_03325"
     CDS             complement(2128..>2530)
                     /gene="porA"
                     /locus_tag="VN478_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HWQ21739.1"
                     /translation="YKTEDADAVIVVLNSAAGTAKVAVDAMRGRGKKVGLLKPILFRP
                     FPYAEVREALKDVPVIGVLDRSMDFGAYAPVYTEMRNVLYELDKKPTTQSYIYGLGGR
                     DVMATEIESVFEELLSGKLDPQHQRYVGLRG"
BASE COUNT          527 a          791 c          705 g          507 t
ORIGIN      
        1 cctcaggcgc gaccatcgtg ctgcgaaagt tgtcaagata gatcattgcc tacctccctg
       61 tcctgccgcc atgcgagaag gaccccgcgc gcaggactgc ctctttcagc ctatcacaaa
      121 cggggcaaag tcaacacgcg aaaaaggggc gaatacgcat caacgcttgg agtggtggcg
      181 gctcagtgcc gcgtatgtcc ctctcatgtc tcggtagtgt cgcagatccg aacgggacag
      241 tgacagcatc tgtcgaacaa cgtcaggctg actcggatat cgctcggcga ttgcccagta
      301 ccccctgaag gccttgtgtt cagcttccac aacagtgtcg agtccctgga gaagggctcc
      361 gtagtcaaga acctgtcctg ccgtcaacga gacgatgggc tccctcccgg gttcatacag
      421 aatgcccgca tatgcacgat acagttgctg caactgctga gtgtgagcgg ccttgtcggc
      481 tgcaagagag ctgaaccttg ctttgaggcg cctgtcggga gtcatctcga caagctggac
      541 gtagcgactg cgcgcttcga cctcatcgtc gatcgcattc tcgagcaact ccaggaggct
      601 tgtggctact ccctgctctt gcgcttcgat acgccgtccg atgcggttgc cgagtgatcg
      661 aagaacccgc ggcccacgcc agaacgtgcg aaggctgaaa acgtcgccga tcgttccacc
      721 ctgcctgaca acgtcgtcct cgagctgggc gacaagttca tccccgccgc tccaccgacg
      781 cgtcatcacc aggaacacgg tgacgccagt aagttcgact gatcggacga atccggatac
      841 ggccggaacg atagccccgt tccagtacgg aaagaccaga acaagcctgt ccccgcgtcc
      901 gaacgaaacg tcgggcgtgc tgagcactcc caggtcaaag ctccggactg cctgcctcga
      961 tgcacgcttc catccgggag gcagggcgct gaaccgatcg gtcaggccaa acaggggaga
     1021 atccagtgtc ttggccgcgc cttcagctac cgtccgcgaa tgccccgtaa cagagtagaa
     1081 cactgtcttc gttgtcatcg attcgctcct atgacgcaat cagcccaccg ccattgtagc
     1141 cggtgggctg atgaatacaa cgcgaacctc aggcgatcta gaactggatc ccggactcct
     1201 caagcttcag caggcgggcc cactcctcat cgatcatggt ctgcatctcg acgatgatat
     1261 gctcgttgcc cggccggaac aggtgcttga agcggctctg gcccttgagg aactcctcga
     1321 ccggcttgcg gttggagggc ttcgtcgtga tgtgcacaac accgttctcg atctcgtaca
     1381 acggccagaa gttggtctcg accgccaatt tggaatactg catcgtcagt gccggatcgt
     1441 aggaccaatt ggtcgtgcat ggctgaaagg cggcaagaac agcagggcca tcagcgttga
     1501 agcccttctg cgccttcttg aacagatcca gcgtcgcgtg caccgccgcc tgaccaacgt
     1561 atggaatacg gtgagccgct gcaatctcca cgaggttctt gcgccactgc ggcttgccga
     1621 agctcacttt tcctgcagga acggtcgacg tgctggcgcc aaatggagtc gcgccactac
     1681 gctggtttcc agtattcatg tagccttcat tgtcgtacac aaggtacatg aacttgtgtc
     1741 ggcgctcgag cgccccggag agagcctgga atccaatgtc ataggtgcca ccgtcgccgc
     1801 cgactgcaag aatgttgaca tcacagtcga tcttgccgcg tcgcttgaga gccttgtatg
     1861 ccgactcaac ccctgctgcc accgcagcag cgttctcgaa ggtcaggtgc atccacggag
     1921 tgttccatgc agtgaacgga tagaccgccg tcgtcacttc caggcacccg gtcgcgttga
     1981 gcacgatgac aggcttgtcg gaagctgcca ggacagtctt gacgatagat gcaatgccac
     2041 agcccgagca catccggtgg cccgaaacga tgcgtgcgtc tgctccagcc gttttgagcg
     2101 caatctcttg aagtgtaggc atctgagcta ccccctcagc ccgacatatc gctgatgctg
     2161 cggatccagc ttgccggaca gcagctcttc gaacacagac tcgatctcgg tggccatgac
     2221 gtcgcggccg cccaaaccat aaatgtagct ctgtgtcgtc ggcttcttgt ccagttcgta
     2281 caggacattc cgcatttctg tgtacacggg cgcataggcg ccaaagtcca tcgaccgatc
     2341 gaggacaccg atcacaggaa catccttgag tgcctcacgg acctcagcat atgggaacgg
     2401 gcggaacagg atcggcttga gcaggcccac cttcttgccg cgccctcgca tcgcgtcgac
     2461 ggcaaccttc gcggtaccgg ccgccgagtt caggaccacg atgacggcat cagcatcctc
     2521 ggtcttgtac
//
LOCUS       DATMZD010000161         5537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_145436,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000161 DATMZD010000000
VERSION     DATMZD010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5537
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145436"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VN478_03330"
     CDS             <1..316
                     /locus_tag="VN478_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018963355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HWQ21740.1"
                     /translation="GSTVLVTNDPIVSAQDVDVVYTDVWASMGQEAEHEERVKVMRPF
                     SLTADLMQHAKKDAIVLHCLPAHRGEEITDEVMDGSQSVVFDQAENRLHIQKAVLALL
                     LG"
     gene            complement(407..1117)
                     /locus_tag="VN478_03335"
     CDS             complement(407..1117)
                     /locus_tag="VN478_03335"
                     /inference="COORDINATES: protein motif:HMM:NF024590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HWQ21741.1"
                     /translation="MALLRVEDAERLRIRFTRDLAGPVTLTLVTSSDTGVTCVPPDAA
                     PPLVNDANRPYLPLARQVVDEVSALSPLITVKVVHHSVVDDTVAEFRITHVPTILVQG
                     ADVDRPLYYVGTPLGYEFTTLVDGIVDASQGKTRLSLLTIDALAKVTEPATIKVFVTP
                     SCPHCPGTARLAQQFGMVNPLIRAEVVEIGEFPDIADQYEVSSVPKSVMNGEHEVVGA
                     LPEAEYLKEFLSAVGVAD"
     gene            complement(1098..2051)
                     /locus_tag="VN478_03340"
     CDS             complement(1098..2051)
                     /locus_tag="VN478_03340"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWQ21742.1"
                     /translation="MDERTIDILIIGAGPAGLAAALYAGRSLVKPVVLERISVGGLVL
                     STEWIDNYPGFPDGIAAPELMERMRRQAERFGAEFVSDEALSLVRTDSGFAVSTAEGT
                     TYKAGSVIIATGATPRVLPVKEESRYRGRGISYCATCDAAFFKGKPVAVVGGGGSAVE
                     EALVLARGCSKVTVVFPLDSLQAEPILVEQLKGLPNVEFVPGAAPAAVEGEGKVERLR
                     VRLAAGGEKTLEVSAVFVAMEFVPTADLLNGLVEVDAQGYVVAPESTETAVAGLYAAG
                     DVRRKSLRQIVTAVADGAVAAQKAGTYVRSRKARQSGAPEG"
     gene            complement(2125..2508)
                     /gene="rsfS"
                     /locus_tag="VN478_03345"
     CDS             complement(2125..2508)
                     /gene="rsfS"
                     /locus_tag="VN478_03345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HWQ21743.1"
                     /translation="MNGKRLANRIVRECERMGAEAVKLVDVRQRTPLFDFLVIATVDN
                     TVLADALLRKIARAVELEDDVAAFTESSPESDWVVSDYGDTVFHVFVGSDIRKRYDLE
                     GLWSSPVRKRRKTSLRSSEDLAGAE"
     gene            2680..3021
                     /locus_tag="VN478_03350"
     CDS             2680..3021
                     /locus_tag="VN478_03350"
                     /inference="COORDINATES: protein motif:HMM:NF020497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HWQ21744.1"
                     /translation="MQETLDRLKSSAGVLAVILTDLDGSILYTASDMDIAPPLTRGMI
                     LSFAGYMQQIVTQLNMGKLTELDVETTTGRIMMVRTNDSVLSVLTTKQTNLGIIRVAI
                     GHALKDLSESA"
     gene            3059..5008
                     /gene="metG"
                     /locus_tag="VN478_03355"
     CDS             3059..5008
                     /gene="metG"
                     /locus_tag="VN478_03355"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HWQ21745.1"
                     /translation="MDRYFLTTAIYYINDKPHLGSVSETIAADFVARYQRKLGRDVLF
                     STGTDEHSQKVERTALAAGQPVEEYTAVAAASWKSLFDRLGISYSRFIRTTDADHMKV
                     VQEMFQKLYDQGDIYKGSYDGWYCVKDEAFLLESDLVDGKCPHCGLEVQHVSEVNYFF
                     RLSRYQDALLAWYEQHPDFVQPQMRYNELVNVMKGGLRDVSVSRSTVKWGVPVPFDTD
                     QTVYVWFDALINYATVAGYGSERFATDWPADLHLIGKDITRFHGIIWPAMLMALNLPL
                     PRHIFAHGFWNFEGEKMSKSLGNVVDPAALVENLSSLIGIDERMAVDVLRYYLCREGT
                     YGLDVDFSSQRLLLRFNTDLANDLGNLLNRTLNMFAKFRPQIAGQPLEPKPALAGLFA
                     STLSAVSGDYGSLQFSAGLEKTWMLVNVLNKMIEDDKPWALAKGGDAAGLAAHLATLL
                     TGLWYVANMIEPCMPTVSDRLLGAIRRPQVIWSDLAWRGPGDDVPEASSTVLFPRIQP
                     DQLDGFLAAQSARKGVPTPPPVGPTPTSTLPVDPGLISIDDVAKVQLRVGTILEGERV
                     PETEKLVKLRVKVGSEERTIVAGIGKHYDPVDLVGKQIVIVANLQPRKLKGITSQGML
                     LAASVGDEMALLTPMAILPDGAEIH"
     gene            5005..>5537
                     /locus_tag="VN478_03360"
     CDS             5005..>5537
                     /locus_tag="VN478_03360"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HWQ21746.1"
                     /translation="MSEKLSFVDTHAHVQMAEFDADREDVLERCWGSGVSWIVCPGVD
                     LITSDLAGQLGEREPRVLAAFGVHPEDCADAPSDYLERLRALFARYGERAVAVGEIGL
                     DFKEGTPDHALQLRFFEEQLQLAADLGLPVIVHSRFAVAECLSAISHFAALRGVMHCF
                     GGTPAEVERAAGMGLCVS"
BASE COUNT         1125 a         1755 c         1583 g         1074 t
ORIGIN      
        1 cggcagcact gtcctcgtga ccaacgatcc catcgtgtcc gctcaagacg tcgacgtcgt
       61 ctacacggat gtctgggctt ccatggggca ggaggcagag cacgaagaac gcgtgaaggt
      121 catgcgtccc ttctctctta ccgccgacct catgcagcac gccaagaagg acgccatcgt
      181 tctgcactgt ctgcctgctc atcgtggtga ggagatcacc gacgaagtga tggacggctc
      241 tcagagtgtc gtgttcgacc aggccgagaa caggcttcac atccagaaag cagttctggc
      301 tcttctcctc ggctgacaat cgtaccgcct cacataacat gaacaaaggc cgggacactc
      361 ccggcctttt catttctgcc catggggctg aggccctcga tgctcgtcag tcggcaacgc
      421 ccacggcaga gaggaactcc ttgagatact cggcctcagg cagagcgcca acgacctcgt
      481 gttctccatt catgacactc tttggaacgg aactgacttc gtactgatcg gcaatgtcgg
      541 ggaactcgcc gatctcgacg acttcagcgc gaatcagagg attcaccatc ccgaattgct
      601 gtgccaggcg ggccgtgccc gggcagtgag gacagctggg cgtcacaaac accttgatcg
      661 tcgccggctc ggtcaccttc gccagggcgt cgatcgtgag gagcgacagg cgcgtcttgc
      721 cctgcgacgc gtctacgata ccgtcgacca gcgtcgtgaa ctcgtacccc agtggcgttc
      781 cgacatagta cagcggcctg tctacgtcgg caccttgaac gagaatcgtg ggcacatggg
      841 taatcctgaa ttcggccacg gtatcgtcga cgacagagtg atgaactacc ttgactgtga
      901 tcagtggtga tagtgcgctc acttcgtcga cgacctgccg tgcaagcgga agatacggcc
      961 tgttggcatc attcacgagc gggggtgccg cgtccggtgg aacacacgtc acgcccgtgt
     1021 ccgacgaagt cacgagcgtc agtgtcactg ggccggccag atcccgggtg aagcggattc
     1081 gcagtcgttc ggcgtcctca accctcagga gcgccactct gccttgcctt cctgctgcgc
     1141 acatacgtgc ccgctttctg ggcagcaacc gcgccgtcag caacggcagt cacgatctgt
     1201 ctcaacgatt tgcgccgaac gtctcctgct gcgtagagtc ctgcaacagc agtctccgta
     1261 gattccgggg ccacaacata gccctgggcg tcaacctcaa ctaatccgtt gagcagatcc
     1321 gcagtgggaa cgaactccat ggcaacgaac actgcggaga cctccagcgt cttctctcct
     1381 ccggcagcga ggcgcaccct cagccgctcc acttttccct caccttcaac ggccgcggga
     1441 gccgcaccag gcacgaattc gacattcggc agtcccttca gctgttcaac caggatcggc
     1501 tccgcctgca gagagtcgag tgggaacacg acagtcacct tgctgcagcc acgagccagc
     1561 acgagcgctt cttccaccgc cgaaccacca ccccccacga cggcgaccgg cttccccttg
     1621 aagaaagcgg catcgcacgt agcgcagtag ctgatgccgc gtccgcgata cctggactcc
     1681 tctttcaccg gaaggactct cggcgtggct cccgttgcaa tgatgaccga gcccgccttg
     1741 taggtcgtcc cctccgctgt cgacacggca aatccactgt ccgtcctgac aagcgacagc
     1801 gcctcgtccg acacaaattc tgcaccgaat cgctcagcct gccgccgcat gcgctccatc
     1861 agttcgggcg cagcaatccc gtcggggaat ccaggatagt tgtcgatcca ctcggtcgaa
     1921 agaaccagcc cccccacgct gatgcgctcc agtacgaccg gcttgaccag cgaccggccg
     1981 gcatatagcg ctgcagcaag gcctgcgggc ccagctccta tgatcaatat gtcgatagtg
     2041 cgttcatcca tcattggcct ccatataccc cacagggtat gtgtaagagt atactgagtg
     2101 tgtgcaggag cacaactgcc gcgtctactc tgctcccgcc aggtcttccg aggaacgaag
     2161 agaggtcttc ctgcgcttcc tgacgggcga cgaccacaat ccctcaagat cgtagcgctt
     2221 ccggatgtcg ctgccgacaa agacgtggaa gacagtatcg ccgtagtcac tgacgaccca
     2281 gtccgattcg ggtgatgatt cggtgaacgc ggcgacatcg tcctcgagtt cgacggccct
     2341 ggcgatcttg cgcagcagcg catcggccag aacggtattg tcgacggtcg cgatcaccag
     2401 gaaatcaaaa agaggggtcc gctgtctgac gtcgaccagc ttgaccgcct cggcccccat
     2461 cctctcgcat tccctgacaa tccggtttgc aagtcgcttg ccattcatgc gcacccctac
     2521 ccgatagttg accctgcaac gtcattgtac ggccggtcac ttcgaaagca acgccggcac
     2581 tgccctccgt tgcacaaacg ccttttgcac gtatcatgtt tgatattcag acgcgtgttt
     2641 cagactacat tccgagagac agaaaggctg gtgatcgtgg tgcaagagac gctagacaga
     2701 ctcaagtcat ctgcaggtgt tctcgccgtc atcctgaccg atcttgacgg ctcaattctg
     2761 tacacggcct cagacatgga catcgcaccg cccctcaccc gcggcatgat cctgtccttt
     2821 gcggggtaca tgcagcagat tgtcacccag ctcaacatgg gcaagctcac tgaactggat
     2881 gtggaaacga cgaccgggcg catcatgatg gttcggacga atgacagcgt tctttccgtc
     2941 ctgacaacca agcagactaa ccttggtatc atcagagtcg ccataggcca tgccctcaag
     3001 gacctctcgg agtctgcctg agcgacacgt cccagccgac atagcaggag gagttgccat
     3061 ggatcgatat ttcctgacca cggccattta ctacatcaac gacaagccgc acctcggctc
     3121 agtgtcagaa acgattgccg ccgactttgt tgcgcggtac cagcggaagc tgggaagaga
     3181 tgtgctgttc tcgacaggca cagatgagca ttcccagaag gtcgaacgga ccgcgctcgc
     3241 tgctggacaa cccgtcgaag agtataccgc agtcgcagcc gcgtcatgga agagtctctt
     3301 cgacaggctg ggcatcagct actcccggtt catccgtacg actgatgcag accacatgaa
     3361 ggtcgtacag gagatgttcc agaagctcta cgaccaggga gacatctaca agggatcgta
     3421 tgacggctgg tactgcgtga aggacgaggc attcctcctc gagtcagacc tcgtggacgg
     3481 caagtgcccg cattgcggcc tcgaggtaca gcacgtctcc gaggtgaact acttcttccg
     3541 actctccagg taccaggacg ctctccttgc ctggtatgag cagcaccctg acttcgtaca
     3601 gccgcagatg cgctacaacg agctcgtcaa cgtcatgaag ggcggccttc gggatgtctc
     3661 cgtgtcgagg tcgacggtca agtggggagt ccctgtcccc tttgacaccg atcagacggt
     3721 ctatgtctgg tttgacgcgc tgatcaacta cgccacggtg gccggctacg gcagtgagcg
     3781 gtttgccact gactggcctg ccgacctcca cctgatcggc aaggatatca cgcggttcca
     3841 cggcatcatc tggccggcca tgctcatggc cctgaacctg ccgctgccga gacacatctt
     3901 tgcccacggc ttctggaact ttgagggaga gaagatgtcg aagtcgcttg gcaacgtcgt
     3961 cgatccagca gctctcgtcg agaacctgag cagcctcatc ggcatcgacg aacgcatggc
     4021 tgtcgacgtc ctgcggtact acctctgcag ggaaggcacc tatgggctcg acgtcgactt
     4081 ctccagccag cgtctcctgc ttcgcttcaa cacggacctg gcaaacgacc ttggcaactt
     4141 gttgaacaga acgctgaaca tgttcgccaa gttccgaccg cagatcgccg gccagccgct
     4201 cgagccgaag ccggctctgg cgggactgtt tgcatctaca ctctcggcag tgagcggcga
     4261 ctatggcagc ctccagttct cagcaggact ggagaagacc tggatgctgg tcaacgtgct
     4321 gaacaagatg attgaagacg acaagccatg ggcactcgcc aagggcggcg acgccgcggg
     4381 gctggccgcc catctggcga cactgctgac aggactctgg tacgtcgcaa acatgatcga
     4441 gccttgcatg cctaccgtct cggatcgtct actgggggca atccggagac ctcaggtcat
     4501 ctggagcgac cttgcctggc ggggtccggg agacgatgtc cccgaggcaa gctcgacggt
     4561 attgttcccg cgtatccagc ctgaccagct ggacgggttt ctcgctgctc agtcagcccg
     4621 caagggcgtc ccaacgccgc cccccgtcgg accaactcca acgtccacgc ttccagtcga
     4681 cccagggctg atctcgatcg acgacgtcgc caaggtgcag ctgcgggtcg gtaccatcct
     4741 cgaaggggaa cgcgtgcctg agaccgagaa acttgtcaag ctccgtgtga aggtcggcag
     4801 cgaggagcgt acgatcgtgg caggcatcgg gaaacactac gatcccgtgg atctggtcgg
     4861 caagcagatc gtgatcgtgg cgaatctcca gccgcgcaag ctcaagggca tcacgtcgca
     4921 gggcatgctg ctggctgctt cggtggggga cgagatggct ctgctgacgc ccatggcaat
     4981 cctgcccgac ggagctgaga tccattgagc gagaagctgt ccttcgtcga cacgcacgcg
     5041 cacgtccaga tggccgagtt cgacgccgac cgggaggacg tgcttgagcg atgctggggc
     5101 agcggcgtga gctggatcgt gtgcccgggc gtcgacctga tcacttcgga cctggcggga
     5161 caactcggcg aacgagagcc gagagtcctt gcggcgtttg gcgttcaccc cgaagactgt
     5221 gccgacgcac catcagacta cctggagcgg ctgcgagctc tcttcgcgcg gtacggagaa
     5281 agggcagtcg cagtcggcga gatcggactg gatttcaaag agggcactcc cgaccacgcc
     5341 ctgcaactcc gtttcttcga ggaacagctc caactggctg ccgacctggg gctccccgtc
     5401 atcgtgcact cacgcttcgc cgtggcggaa tgtctgagcg ccatctcgca cttcgcagcc
     5461 ttgcgtggcg tcatgcactg ttttggtggt accccggccg aagtcgagcg cgctgccgga
     5521 atgggactct gtgtgtc
//
LOCUS       DATMZD010000162         2052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_74674,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000162 DATMZD010000000
VERSION     DATMZD010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74674"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            537..929
                     /locus_tag="VN478_03365"
     CDS             537..929
                     /locus_tag="VN478_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018251794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HWQ21747.1"
                     /translation="MMEFYVNPYDGVPVYLQIIQLVRNGVALGTLHPGDQLPTVRELA
                     VKLAVNPNTVAKAYRDLEREGMVETVSGKGTFVREGRIGVETERLEHMLEMLIVEARN
                     AGLSIDDLIERLEKRKQTSAKARDQGGS"
     gene            929..1810
                     /locus_tag="VN478_03370"
     CDS             929..1810
                     /locus_tag="VN478_03370"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWQ21748.1"
                     /translation="MNVIEMVGIDKSYGQVKALQDMCLTVPAGSIFGLMGPNGAGKTT
                     SIKLMLGLMEPTAGSLTVLGVVPTGDGTVLRRRIGFVPEDFSLYPQMTGFEILQFNAR
                     LYGCSVDENVRRLQTVFELPLPRRISTYSKGMRKLLGLYIALSTEPELLILDEPTDGL
                     DPVVRSHFLGVLADETSRRNLTVFFSSHILSEVEKICDTVAFMRSGHTVLQDEVESIK
                     SQYRVYTVRFEAGHSLRDVKGITIQRERSLGPDTWDVEAMADQEQARSAFADAGGVVL
                     NMQSLPFDEIFLRFVEA"
     gene            1812..>2052
                     /locus_tag="VN478_03375"
     CDS             1812..>2052
                     /locus_tag="VN478_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21749.1"
                     /translation="MKALMWKELREDRLKIIIGCVLLSVIGLAISIAYRFLEGGIPGM
                     GQIPPEYADLIKKLMPDFSNVNAYLYSQWFSKNLQQ"
BASE COUNT          414 a          558 c          627 g          453 t
ORIGIN      
        1 tactggcccg cctgtctgtc cgccgaggcc ctcaccgcgc ctctccacgt tatcgctgcc
       61 cgaaccgaca tgtttctctc tgccgaccgg tctgttgttt ctgtccatcc ggcaacacct
      121 cctggcatgg cgtcttctgg cgaagaactc tactagcaac tatacagctg gcgcgcgatg
      181 tgtccaccct tctgaagact gcgatttcga cagactcctg cggcacagaa ctggcgaacc
      241 cccagtcagc gaccctgaca gggagtctgt actccgattc ctgcactgtc gtgactatgt
      301 gcgtgactcc gtccgatcgg gtgtccagtc caagtggttc tgcgacactt cgaagagaca
      361 gaagcgttct tctatggcgg atggccgatg cagcaactgg gtcggtgtcg ctgttgagag
      421 gccgcgtctc tgtctgctgc ggttcctccg attcacgggt tcgcggtcat gccggttctc
      481 agtcaactct tgacaatctt gcatattgta ctatcgtaat agtacaataa ggggtgatga
      541 tggaattcta tgtgaaccca tatgacggcg tacccgtgta cctgcagatc atccagctgg
      601 tcaggaatgg tgtggctcta ggcacgctgc acccgggcga tcagctgccg acggtacgtg
      661 aactggccgt caagctggct gtcaacccga acacggtcgc caaggcctac agggacctgg
      721 agcgcgaggg catggttgag accgtgagtg gcaaggggac gtttgtgcgc gaaggcagga
      781 tcggcgtgga gacagaacgc cttgaacaca tgctggaaat gctgatcgta gaggctcgca
      841 acgccggcct ctcgatcgat gaccttattg agcggctgga gaagcgcaag cagacttctg
      901 cgaaagcgcg tgatcaggga ggcagttgat gaacgtgatc gaaatggttg gcatcgataa
      961 gtcgtatgga caggtgaaag cgctgcagga catgtgtctg actgtcccgg ccggaagcat
     1021 cttcggcctt atgggaccca atggggcggg caagactacc agtatcaagc tgatgctcgg
     1081 gctgatggag cctactgctg gttcgctgac tgtccttggc gtcgtcccga caggagacgg
     1141 gacagtcctt cgtcggcgca tcgggtttgt tcccgaggac ttctcgctct atcctcagat
     1201 gacaggcttc gagatactgc agttcaacgc gcggctgtac ggctgctctg ttgacgagaa
     1261 cgtgcggagg ctgcagacgg tcttcgaact gccactgccg cgcaggatct cgacgtactc
     1321 caaggggatg cgcaagttgc ttgggctcta cattgctctc tcgacagagc cggagctgct
     1381 catcctcgac gagccgaccg acggcctgga ccccgtagtc aggtcgcatt tcctgggtgt
     1441 gctggcagac gagacgtccc ggcgcaatct gacggtgttc ttctcatcgc acatcctcag
     1501 cgaggtcgag aagatctgtg atacggtggc gtttatgcgg tccggtcaca cggtccttca
     1561 ggacgaggtg gagtctatca agtcgcagta tcgtgtctac acagtccggt ttgaggcggg
     1621 acacagcctt cgcgacgtca agggtattac catccagcga gagagaagtc tgggcccgga
     1681 tacgtgggac gtcgaggcaa tggccgatca ggaacaggcc cgttcagcgt ttgctgacgc
     1741 ggggggtgtg gtgctcaaca tgcaatcgtt gccgtttgac gagatattcc tgcggttcgt
     1801 ggaggcatga cgtgaaagca ttgatgtgga aggaactgcg ggaagaccgc ctgaagatca
     1861 tcataggatg cgtgttgctg tcagtgatag gactggccat ctccatcgcg tatcgttttc
     1921 tcgaaggcgg tattcccggc atgggccaga tcccgccaga gtacgcggac ctgatcaaga
     1981 agctgatgcc ggacttcagc aatgtgaacg cctacctgta ttcccagtgg ttcagcaaaa
     2041 acctgcaaca gg
//
LOCUS       DATMZD010000163         2479 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_74682,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000163 DATMZD010000000
VERSION     DATMZD010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74682"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2479)
                     /locus_tag="VN478_03380"
     CDS             complement(<1..>2479)
                     /locus_tag="VN478_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0051731 - polynucleotide
                     5'-hydroxyl-kinase activity [Evidence IEA];
                     GO_process: GO:0042245 - RNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polynucleotide kinase-phosphatase"
                     /protein_id="HWQ21750.1"
                     /translation="AVVALIGASGSGKSTFAKAHFKPTEVLSSDYFRALISDDESNQK
                     VTAEAFDTLYYVANKRLASGLMTVIDATNVQKEARASVLSLAKEQNCHAVAIVLDIPE
                     EICRERNEKRPERNFDGRVISRQTRQLRQSVKSLRREGFRYVFVLTNEKEATDAEIVR
                     VPLWNNKRNETGPFDIIGDIHGCYDELVELLSKLGYAVCADSCTAKPPEGRKAVFVGD
                     LCDRGPKNVEVLRLVMNMAQAGDAWCVAGNHDAKLLKSLKGATVQMSHGLDRTVEQLA
                     AESEEFVTSARSFLDGLISHYVFDDGRLVVAHAGLKEKYQGRGSGRVRDFCLYGDTTG
                     EIDQYGLPVRLPWADDYRGEARVVYGHTPVPEVQIVNNTVCIDTGCVFGGRLTSYRYP
                     EKEIVQVDAREEYCVPVRPVLDRPIQDDDLLNIDDVLGQRFLNTRLRHSIKISAESSA
                     AALEVMSRFAADPHWLIYLPPTMSPCETSNIPEYLEYPTEALDYYRTRGISKVVCEQK
                     HMGSRAVTVLCKNADIAAKRFGVDDGSSGIVYTRTGRHFFDDVQTETAILARLQKVLT
                     ASRFWDDFDTGWVCLDTELMPWSAKAQKLLEEQYAPVGRAGRSGLALAVDAIEKAART
                     LDGTSVDAETRPSQETDLTELLERYTERQETLSLYTDAYRRYCWNVEKLEDYRIAPFH
                     ILATEGKTWCGENHIWHMETIAKYVTGIDPIFMPTKHLLVDLLDENSVAASVRWWEDL
                     TTSGGEGMVVKPYDFIATRGADLLQPAIKCRGREYLRIIYGPEYTLGGNMERLKQRSL
                     SKKRNLALNEFALSMESLERFAQREPLF"
BASE COUNT          492 a          734 c          663 g          590 t
ORIGIN      
        1 gaatagtggt tcacgctgcg caaagcgttc cagcgactcc atactcaggg cgaactcgtt
       61 gagtgccaga tttcgcttct ttgagagtga ccgttgtttc agccgttcca tgttgccacc
      121 cagcgtgtac tccggcccat agatgatacg gaggtattca cggccacggc acttgatggc
      181 gggctgcaac aagtctgcac ccctggttgc gatgaaatca tagggcttga ccaccattcc
      241 ctcgccgcca gaagtggtca gatcctccca ccatcggacg cttgctgcta cgctgttctc
      301 gtccagcaag tcgacaagca ggtgctttgt cggcatgaag atgggatcga ttccggtcac
      361 gtacttggcg atggtttcca tgtgccagat gtggttttcg ccgcaccagg tcttgccctc
      421 cgtggcaagg atatggaagg gagcgatacg atagtcttcg agcttctcca cattccagca
      481 atagcggcga taggcgtcgg tatagagcga cagtgtttcc tgccgttccg tgtatcgttc
      541 gagaagctca gtcagatcag tctcttgact tggtcgtgtc tccgcgtcaa cagatgtgcc
      601 atcaagggtt ctggccgcct tttcgatagc atccactgca agcgcaaggc cgctgcgtcc
      661 agcgcgtccc actggcgcgt actgctcttc cagcagcttc tgtgctttgg ctgaccaagg
      721 catcagctcg gtgtcaaggc aaacccatcc cgtgtcgaaa tcgtcccaga accgagatgc
      781 agtcagaacc ttctgcaatc gcgccaagat ggcagtctcg gtctggacgt catcgaagaa
      841 gtgacgcccc gtgcgggtat agacaatgcc ggagctgccg tcatccacgc cgaaacgctt
      901 tgctgcgatg tcagcgttct tgcacagcac tgtcactgcc cgcgaaccca tgtgcttctg
      961 ttcacagact accttgctga tgcccctggt cctgtagtag tcaagcgctt ctgtcggata
     1021 ttcaagatac tccggaatat tgctcgtctc acagggggac atggtaggcg gaaggtagat
     1081 gagccagtgc gggtcggcgg caaatcgact catgacctca agtgcagcgg cgctgctctc
     1141 tgcgctgatc ttgatggagt gacgcagccg agtattcagg aatcgctgtc ccagcacgtc
     1201 gtcaatgttg agaaggtcgt catcctggat cggtcgatcg aggactggtc tgaccgggac
     1261 acaatactcc tctctcgcat ccacctggac gatttccttc tcgggatacc gatagctcgt
     1321 cagcctgccg ccaaacacac agccagtatc gatgcaaacg gtgttgttga cgatctgtac
     1381 ttccggcaca ggcgtgtgtc cataaaccac ccgtgcctcg ccgcggtaat catcagccca
     1441 gggcaagcgc accggcagac catactggtc gatttcgccg gtagtatcgc catataggca
     1501 gaaatcgcgc acccgtcctg atcctcggcc ctggtacttc tccttgagac ccgcatgagc
     1561 aactaccagt ctgccatcgt cgaagacgta atggctgatg agaccatcaa ggaaactcct
     1621 tgcacttgtc acgaattcct cgctctctgc tgccagctgt tccaccgtcc tgtccaaccc
     1681 atgactcatc tgcacggtgg cgcccttgag gctcttcagc agtttggcat cgtgattgcc
     1741 ggcgacacac caggcatcac ctgcttgcgc catgttcatg acgaggcgca gaacctccac
     1801 gttcttcggt cccctgtcac agagatcacc gacgaacact gccttgcgcc cctcgggggg
     1861 tttggcagtg cagctgtccg cgcaaactgc ataccccagt ttggacagca actcaacgag
     1921 ctcatcgtag cagccgtgga tgtcgccgat gatgtcgaac ggtcctgtct cgttcctctt
     1981 gttgttccac aagggcacgc gaacgatctc ggcgtctgtg gcttcctttt cgtttgtcag
     2041 cacaaaaaca taccggaagc cttctctccg aagagacttg acggattgcc tgagttgtct
     2101 ggtttgccgg ctgatcacgc ggccgtcgaa attgcgttca gggcgcttct cattgcgctc
     2161 gcggcagatc tcttctggaa tgtccagcac gatagcaacg gcatgacagt tctgttcctt
     2221 tgccaggctc agtactgatg cccgggcttc cttctgcaca tttgtcgcgt caatgactgt
     2281 cattaacccc gacgccagcc gcttgtttgc cacatagtag agcgtgtcga aagcctctgc
     2341 agttactttc tggttgctct cgtcgtccga gatgagagcg cggaaatagt ctgaagacag
     2401 gacttccgtg ggtttgaagt gcgctttggc aaaggtggat tttccgctgc ccgatgcacc
     2461 tatcagtgcg actacggcg
//
LOCUS       DATMZD010000164         2265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_24723,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000164 DATMZD010000000
VERSION     DATMZD010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24723"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..992)
                     /locus_tag="VN478_03385"
     CDS             complement(<1..992)
                     /locus_tag="VN478_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HWQ21751.1"
                     /translation="MFDKESVGRLKAAKSKWQQGADKVPAGKTCKAYSDCATSSGIDV
                     KLLYTPEDVGEMDYMRDLGFPGEYPYTRGYQATMYRGRLWTMRQYAGFATVEESNRRY
                     KYLLNQGQTGLSIAFDLPTQLGMDADHPLSEGEVGKVGVSVSSLADLEVLFDGIPLDQ
                     VSTSMTINAPAAVLLAMYIALARRKGISEDKLNGTIQNDILKEYSARGTYIYPPAPSM
                     RIINDIFAYCSKEVPNWNTISISGYHMREAGCTAVQEVAFTLADGIAYVEAAVRAGLD
                     VDSFAARVSFFFNAHNNLLEEVAKFRAARRMWARIMKDRFHAQNPKSLMLRFHT"
     gene            1313..1837
                     /locus_tag="VN478_03390"
     CDS             1313..1837
                     /locus_tag="VN478_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HWQ21752.1"
                     /translation="MEQHGLVMVYTGNGKGKTTAALGLGLRAAGAGQRVLLVQFMKGD
                     PTYSELKGVAYLPGFEVVQTGLHHWITKDQASPLDKTEAQRGMNVARNAFAEERHELV
                     ILDELNCAIDYGLIPEADVLELLSHRPPFMSVCITGRGAPASLLDAADMVSEVVEIKH
                     HFHQGIPAQKGIEL"
     gene            1845..>2265
                     /locus_tag="VN478_03395"
     CDS             1845..>2265
                     /locus_tag="VN478_03395"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWQ21753.1"
                     /translation="MNPSKRNASGLFNLGAAATIWGSTFVVSRYALASVSFLHLVILR
                     FACSTLLFLVLFLVQRPRLPHGREWWLYLGSAASGHVLSIPFQFLGTSLTSAHIGSII
                     TCATPLVMVVLAGLFLHERIRLRDVAAIVVALAGVALI"
BASE COUNT          426 a          706 c          674 g          459 t
ORIGIN      
        1 tgcgtgtgaa accgcagcat gagactcttg gggttctgtg cgtggaaacg gtccttcatg
       61 atccgcgccc acatgcggcg ggcagcgcgg aacttggcga cctcttccag caggttgttg
      121 tgcgcgttga agaagaatga cacgcgcgct gcgaagctgt cgacgtccag tcctgccctg
      181 acggcagcct cgacatatgc gatcccgtcg gcaagggtaa atgcaacctc ctggacggcc
      241 gtgcatccag cctcacgcat gtgatagccg ctgatgctga tcgtgttcca attgggaacc
      301 tctttcgagc agtaggcgaa gatgtcgttg atgatgcgca tcgacggggc cggagggtag
      361 atatacgtgc cacgagcgct gtattccttg aggatatcgt tctggatcgt gccattcagc
      421 ttgtcctcgc tgatgccctt gcgtctcgcg agcgcaatgt acatcgccag cagcaccgcg
      481 gcaggagcat tgatggtcat ggacgtgctg acctgatcga gtggaatgcc gtcgaacaac
      541 acctccagat ccgcgagcga actgaccgag acgcccacct tgccgacctc gccctccgac
      601 agtggatggt ccgcatccat gcccagctgg gtaggaagat cgaatgcgat ggagagaccg
      661 gtctggccct gattcagcag gtacttgtac cggcggttgg actcctcgac ggtcgcgaac
      721 cctgcgtact ggcgcatggt ccacagacgg ccgcgataca tcgtggcctg gtatcctctc
      781 gtgtacggat actcccccgg aaacccaagg tcgcgcatgt agtccatctc gccgacgtcc
      841 tcgggtgtgt acagcagctt gacgtcgatg ccggaactcg tcgcgcagtc cgaataggcc
      901 ttgcacgtct tgccggcggg aaccttgtca gcgccttgct gccacttgga ctttgcggcc
      961 ttcagtctcc ccacactctc cttgtcgaac atccgatcac ctcacctttg acatgtccgt
     1021 gatgagccct gggcaccctt gcacccgctg cccgtctcca agaaagtata gcaggaaaca
     1081 ggggtctcgc cacgtgcgcg gcagcaggag tgacagagac aagatggatt cccgccttcg
     1141 ccgaggaaca ccactcgcat tggaaagaaa tgctcgtgag gagcacccct cggcttgaac
     1201 aaaagggctc gggggggaat gacggaggca ggcgatttct acccgtaggg gaggctggtg
     1261 ttgttcgcgt gctcgctata ctacagatga cattcctgca gcggaggcgg caatggaaca
     1321 gcatggtctg gtgatggtgt atacgggcaa cggcaagggg aagacgaccg cagcgctggg
     1381 tctcggcctc cgggctgccg gagcggggca gcgcgtcctt ctcgtccagt tcatgaaggg
     1441 ggacccgacc tactccgagc tgaagggcgt cgcctacttg ccgggctttg aggtcgtgca
     1501 gacgggtctg caccattgga tcaccaagga tcaggccagc ccactggaca agacggaagc
     1561 acagcggggg atgaacgtcg cccgcaacgc gttcgcggag gaacgacacg agcttgtcat
     1621 ccttgatgaa ctcaactgcg ccatcgacta tggcctgatc cccgaagctg acgttctcga
     1681 actgctgtcg cacagacctc cgttcatgtc ggtctgcata acgggtcgcg gggctccggc
     1741 aagtctgctg gacgccgccg acatggtcag cgaggtcgtc gagatcaagc accacttcca
     1801 ccagggcatc ccggcacaaa aggggatcga gctctagcag cgtggtgaac ccatcgaaac
     1861 gcaatgcctc ggggctgttc aacctcggag cggcggctac tatctggggc agcacgtttg
     1921 tggtcagccg ctatgccctg gccagcgtgt cgtttctcca ccttgtcatc ctgcggttcg
     1981 catgctctac actcctgttc ctcgtcctct ttcttgtcca gcgaccacga ctgccccacg
     2041 ggcgcgaatg gtggctctac ctcggcagcg ctgcatccgg ccatgtcctc tccatcccat
     2101 tccagttcct cggcacgtcc ctcaccagcg cacacatcgg ctccatcatc acctgtgcca
     2161 cgccactcgt catggtggtc cttgctggac tcttcctgca cgagcgcatt cgcctgcggg
     2221 acgtcgccgc aatcgtcgtc gctctcgcgg gggtcgccct gatat
//
LOCUS       DATMZD010000165         2991 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_55239,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000165 DATMZD010000000
VERSION     DATMZD010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55239"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(207..1283)
                     /locus_tag="VN478_03400"
     CDS             complement(207..1283)
                     /locus_tag="VN478_03400"
                     /inference="COORDINATES: protein motif:HMM:NF015382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carcinine hydrolase/isopenicillin-N
                     N-acyltransferase family protein"
                     /protein_id="HWQ21754.1"
                     /translation="MENRSMPDSGRKGRRLLTSLRIAVVVGLVLVVCSPTAARAPTLP
                     PTSDSTLSVQEIATLSSLEKVDSHPLYTMHFYGSTELSLSEPASGTGSVSTDASFTWG
                     CALFAALGGTEKTYGRNFDWEASPALLLFTHPTSGYASVSMVDLAYLGFGEDVDRLTE
                     LSLDDRKALLRAPSLPFDGMNDHGLVVGMAAVPAGDMQPDPDKDTVDSLMVMRLILDQ
                     AASVDEAVAVLRRYNIDWGGGPPLHYLIADRSGRAALVEFYQGSVHVIPNDKTWHLAT
                     NFLVTSVTGGVSEQCDRYDKASQTLSASAGSLNAQDAMSLLETVSQPHTQWSVVYQMN
                     TGDVSITMGRKYGHSNTFQLDLAR"
     gene            complement(1319..2074)
                     /locus_tag="VN478_03405"
     CDS             complement(1319..2074)
                     /locus_tag="VN478_03405"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWQ21755.1"
                     /translation="MRPSYLLAAMLCVLPILVSGCAAATPPSTNITLQFIGNSCTLIT
                     APDGTRIVSDPYGEFEHPAGLDTLPDDLAANAVTVSHAHPDHNNVEGVGGAPQVISAA
                     GTYQVGMVKITGYAGYEGSPSGPSDNPHVVFVFEIAGVKIVHMGDSGPVTDPVVLAAV
                     ENPDVLLANIDGYVIPSDQLVPFLRQAKVRTFIPTHYSLSTSARWQDAPTVEEFIGTL
                     PTDMTVVRKGSTIQVTPDMPGQVAVLTPLALIK"
     gene            complement(2232..2804)
                     /locus_tag="VN478_03410"
     CDS             complement(2232..2804)
                     /locus_tag="VN478_03410"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HWQ21756.1"
                     /translation="MSQENDGAMDLPVITKGTRVVLTPQRRENVPTYWKWICDPEVNA
                     GLTDAGACVSVENEYEWYDNNVAKATESSVNFDIHEVDGMRLVGDCALFDIDHAAGTA
                     EVGLIIGEKDCWNHGYAAEAASLLARYAFDVRGLISVILHVYDFNERAIAAYRKAGFR
                     EIGRRRNVRHGSRIVQEIIMDIVPGEVAAI"
     gene            complement(2804..>2991)
                     /locus_tag="VN478_03415"
     CDS             complement(2804..>2991)
                     /locus_tag="VN478_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HWQ21757.1"
                     /translation="LILSVFFAGLALATVASIITLTAGSNAFRRKIREMVITGLGAAA
                     ASYAFGRLVQAVFGIHG"
BASE COUNT          560 a          899 c          939 g          593 t
ORIGIN      
        1 agcttgctcg ccacgcgctc gagagacggg gatttgcacg atctgccctc aggatagtcc
       61 gtgcgctgtg tgaatcaacc gcgatcacgc gcacccatcg atttctgagc agtacctgtg
      121 cattggcggc tggcattccc gctcgatgtc tgcgctatgg ccaccggcag acaccgttcg
      181 aggcgtctgc cttcccggct ggagtgtcag cgtgcgagat cgagctggaa ggtgttcgag
      241 tgaccgtact tgcgccccat cgtgatgctg acgtcgcccg tgttcatctg atagaccacg
      301 gaccactggg tgtggggctg agatacagtc tccaggaggc tcatcgcgtc ctgggcattc
      361 agagatcctg cacttgccga gagagtctgg cttgccttgt cgtatcggtc gcattgctct
      421 gagacgcccc cggtgacgga agtgaccagg aagttcgtcg ccaggtgcca ggtcttgtca
      481 ttgggaatga catggaccga gccctggtag aactcgacaa gcgctgccct cccggaccga
      541 tctgcaatga ggtagtgaag cggcggaccg ccgccccagt cgatgttgta gcgccggagg
      601 acggctactg cctcatctac gctggccgcc tgatccagga taaggcgcat caccatcagc
      661 gaatcgaccg tgtccttgtc agggtcgggc tgcatgtccc cggccggcac tgccgccata
      721 ccgaccacca ggccgtggtc gttcatgccg tcgaacggca gcgaaggggc tctcagcaag
      781 gccttccgat cgtcgagcga cagttcggtc agcctgtcca cgtcttcgcc gaatcccagg
      841 taggccagat ccaccatgga gactgaagca tagccacttg tcgggtgagt gaagaggagc
      901 agtgctgggc tcgcttccca gtcaaagttg cggccgtagg tcttctctgt cccgcccagc
      961 gctgcgaaca gcgcgcatcc ccacgtgaac gaggcgtctg tgctgaccga gcctgtgccg
     1021 gatgcgggtt cggatagact cagttcggtt gaaccgtaga agtgcatcgt gtacagcgga
     1081 tgactgtcga ccttctccag gctgctgagg gtggcgatct cctggactga gagcgtgctg
     1141 tccgatgtcg ggggaagagt cggggcacga gctgccgtcg gactgcagac caccaggacc
     1201 aggcctacga ccactgcgat cctcagcgac gtgagaagac gcctgccctt cctcccggag
     1261 tcaggcattg aacggttttc catggaccct ccctcgtatc agtctcagcg agtgtacgtc
     1321 acttgatcag cgccagcggc gtgagcacgg ccacctgccc aggcatgtcg ggcgtcacct
     1381 ggatcgtgct gcccttcctc acgacagtca tgtcggtggg caaggttccg atgaactcct
     1441 ccacagttgg cgcatcctgc cagcgggcgc tggtactgag actatagtga gtagggatga
     1501 atgtgcggac cttagcctgc cgcagaaatg ggacaagctg gtctgacgga atgacgtacc
     1561 cgtcaatgtt ggcaagcagg acgtccgggt tctcgacggc ggccaggacg acgggatcgg
     1621 tgacaggacc cgagtctccc atgtggacga tcttgacgcc agcgatctcg aagacgaaga
     1681 cgacgtgggg attgtcggac gggccagacg gtgacccctc gtagcctgca taccctgtta
     1741 tcttgaccat gccaacctgg tacgtcccag ctgcggagat gacctgaggc gcccccccaa
     1801 ctccttcgac gttgttgtgg tcagggtgag cgtggctcac ggtgacggcg ttcgcggcca
     1861 ggtcgtccgg cagtgtgtcc agtccggccg gatgctcgaa ttcaccgtag ggatcactga
     1921 caatgcgtgt gccgtccgga gccgtgatca gcgtgcatga gttgccaatg aattgaagcg
     1981 ttatgtttgt ggacggaggg gttgcagcgg cgcatccgga tacgaggatc ggcaatacgc
     2041 agagcatcgc ggctagcagg taacttggtc tcataacgat tctgcctcca cacggggttg
     2101 acacgaagaa acgtccggca agtgtcaaga tacatgtgat tctgcgctca ggataggcac
     2161 ggagcgaagt gcgtcaagta ctgctgcaca gccggcaccc tggagacggg tagcggcaga
     2221 tgcagccgtc ctcagattgc tgcgacctcg cccggcacga tgtccatgat gatctcctgc
     2281 acaatacgcg atccgtgccg gacatttctg cgtcggccga tctcgcggaa tccggccttg
     2341 cggtaagccg cgatagccct ctcgttgaag tcgtacacgt gcaggatgac gctgatcagc
     2401 ccccggacgt cgaaagcata gcgggccagc agggaggcgg cctcggccgc atacccgtgg
     2461 ttccagcagt ccttctcgcc gatgatgagc cccacttcgg ctgtgcccgc tgcatggtcg
     2521 atgtcgaaca gggcacagtc tcccaccagg cgcatgccgt ccacctcatg gatgtcgaag
     2581 ttcacactgc tctcagtggc cttggccacg ttattgtcat accactcgta ttcgttctcg
     2641 acggacacac atgcgcctgc gtccgtcagc cccgcgttga cctccgggtc gcagatccac
     2701 ttccagtacg tcggcacgtt ctcccgccgc tgcggagtca gtacgactct ggtgcctttg
     2761 gtgatgacgg gaaggtccat ggcgccgtcg ttctcctgac tcatcatccg tgaatgccga
     2821 acactgcctg cacgaggcgg ccgaaggcgt aggaagcggc tgcggcgccg aggccggtga
     2881 tgaccatctc gcggatcttg cggcggaagg cgttgctgcc ggccgtgagg gtaatgatac
     2941 tggcgaccgt ggcgagggcg agtccagcaa agaagacgct cagaatcaac g
//
LOCUS       DATMZD010000166         4535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_136026,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000166 DATMZD010000000
VERSION     DATMZD010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4535
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136026"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="VN478_03420"
     CDS             complement(<1..425)
                     /locus_tag="VN478_03420"
                     /inference="COORDINATES: protein motif:HMM:NF019976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HWQ21758.1"
                     /translation="MAQVHGRAGEATYRKGDRLMLRALVPMMAMGVLTYFFFRSGIGA
                     NGLFGAGEACVVAAFGFWCVRDVLRLETAADRYYGGAGGEFDVGATLSELPDEFHVFN
                     GLDFYAGDVDHVVVGPTGVFVVETKNHSGTISLKDGYLC"
     gene            628..1887
                     /gene="hisS"
                     /locus_tag="VN478_03425"
     CDS             628..1887
                     /gene="hisS"
                     /locus_tag="VN478_03425"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HWQ21759.1"
                     /translation="MIKSVKGTSDITAPEVLLWQHVERIMATTAKAYGYEEIRTPTIE
                     MEELFSISVGEGTDIVEKEMYAFPDKKGRRLVLRPEGTAASCRAYVEHQMNLQPSPTK
                     FYYVGPFFRYEQPQSGRKREFHSFGVEALGEQSPLQDVEVIDMVMMMLSRLGLAHLSV
                     EINSIGDDNCRPAYKDALRAALAAHESELCDDCKRRMNDNPLRVLDCKREDPSLLASL
                     PKSADYLCADCQDHFHRLLLTLDALRIPYHLNNRLVRGLDYYTRTVFEVISEDLGAQN
                     ALLGGGRYDYLVRHLGGPATPGVGWGLGLERLTSVLTARYPDLTASLAIPRAYVVHSD
                     AATAAAFELRRILADTGWAIDLDARQDGFGKQLARASKRGARMALILGEDESAAGTCT
                     VKDLTSGSQQTVAVTDVAGCLAEAGRT"
     gene            1960..2135
                     /gene="ssrS"
                     /locus_tag="VN478_03430"
     ncRNA           1960..2135
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VN478_03430"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            2166..2627
                     /locus_tag="VN478_03435"
     CDS             2166..2627
                     /locus_tag="VN478_03435"
                     /inference="COORDINATES: protein motif:HMM:NF016160.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HWQ21760.1"
                     /translation="MQTDRGLVVSRHGDVVTVRLQPHAECASCSLSKFSLTQFCGGSK
                     ESSATVKATAAPGIAQGDLVEVSLDDSITLKAAVIVYGLPLVAFLVGVLGGYALSSLI
                     TLRGTLSTAAPVLAGFLMLVPGVVLSRKAARRLNPTATIVGKLDQEVTTCQ"
     gene            2624..3013
                     /locus_tag="VN478_03440"
     CDS             2624..3013
                     /locus_tag="VN478_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21761.1"
                     /translation="MNAAAIWVLDIIIALGLIFMAVAALVVGRKLQEFSKSLSATMAS
                     VGHQADELQSEAIRLMQSTQVSEQHFDQLSRQLVKLTASADTVVKALPAVASGRDSGL
                     LPRVMSTVVGVVSAYRFFRNIFPRRKS"
     gene            3010..3255
                     /locus_tag="VN478_03445"
     CDS             3010..3255
                     /locus_tag="VN478_03445"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HWQ21762.1"
                     /translation="MSERNDCGSFMGGLLMGGIIGALVALVLAPQSGEETRKLLKDKA
                     FELGKEISDQTETIKEKGRELLTQDGIEIGESTDTNP"
     gene            3326..3399
                     /locus_tag="VN478_03450"
     tRNA            3326..3399
                     /locus_tag="VN478_03450"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3358..3360,aa:Gln,seq:ctg)
     gene            3405..3479
                     /locus_tag="VN478_03455"
     tRNA            3405..3479
                     /locus_tag="VN478_03455"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3438..3440,aa:Glu,seq:ctc)
     gene            complement(3568..4314)
                     /locus_tag="VN478_03460"
     CDS             complement(3568..4314)
                     /locus_tag="VN478_03460"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWQ21763.1"
                     /translation="MDIGEEGKPFSAKGAGRVGCLVMQGFTGTVGSIRYYAEQLAARG
                     LSVEAPRLSGHGTKWQDLNKVHYSDWIRDAEQAYQLLAGRCDRVFVTGLSMGGTLVLY
                     LAEHHPEIAGVIPINAPVFMTDKRAPLLPVLKYLVASTPAIASDIMEPGIVEPAYDRT
                     PTSGAHEMVKLLGVTRRDLGLIAQPLLVMRSTHDHVVPHDCAPHILEHVASTDKEMVV
                     FERSAHVVTMDYDKDGVVDSAWRFIQRLSH"
BASE COUNT          948 a         1308 c         1280 g          999 t
ORIGIN      
        1 ctgcacaggt atccgtcctt cagactgatg gtcccggagt ggttcttcgt ctcgacgaca
       61 aagacgcccg tcggaccgac aacgacatga tcgacgtcgc cagcgtagaa atcgagaccg
      121 ttgaacacgt gaaactcatc ggggagctcc gagagagtgg cgccaacgtc gaattcacca
      181 cccgctcctc cgtagtaccg gtcagccgcc gtctccagtc gcaacacatc gcgcacgcac
      241 cagaacccga aggccgcgac gacacatgcc tctccagcgc caaagaggcc gttggccccg
      301 atccctgatc gaaagaagaa atacgtgagg actcccatgg ccatcatggg gacaagagca
      361 cggagcatta gccggtcccc ttttcggtat gtcgcttctc cggccctgcc gtgaacctga
      421 gccattctct ctcctgggat gagacgattg agcgccgtga tagtgcctta agtgtagcat
      481 ccctctcttg gcacacaacc ctgcgttgcc gatggaccat cgaccgtctc agccagcgtg
      541 cagtcaggcc cgtctcccgc atgcgtgtcg gttttctttt cggggacagc aagtatactg
      601 actagcacaa ttgcctggag gatcgtcatg atcaagtcag ttaaggggac cagcgacata
      661 acagcacctg aagttcttct ttggcagcac gtagaaagga tcatggccac gactgccaag
      721 gcctacggat acgaagagat ccgcactcca accatcgaaa tggaggaact cttctccata
      781 tctgtcggag aaggcactga tatcgttgaa aaggaaatgt atgcctttcc ggacaagaag
      841 ggacgccgtc tcgtccttcg tcctgaaggg acggcagcat cgtgcagagc ctatgtagag
      901 caccagatga acctgcagcc ttccccgacg aagttctact atgtcggccc attcttccga
      961 tacgaacagc ctcagagcgg gcgcaaacgg gagttccaca gcttcggcgt agaggctctt
     1021 ggcgagcagt cgccgctcca ggatgtcgag gtcatcgaca tggtcatgat gatgctctcg
     1081 cgactcggcc ttgcacacct gtcagtggag atcaacagca tcggcgacga caactgccga
     1141 ccggcataca aggatgccct gcgggctgct ttggcagcac atgagagcga gctgtgcgat
     1201 gactgcaaga ggcgcatgaa cgacaatcct ctccgcgtgc ttgactgcaa gcgtgaagat
     1261 ccttcgcttc tggcgtcgct gccaaagtcg gcagactatc tgtgtgcaga ttgccaggac
     1321 cactttcatc gcctgttgct cacgctcgat gcactgagga ttccctatca tctcaacaac
     1381 agactggttc gtggtctgga ctactacact agaactgtct ttgaggtcat atccgaggat
     1441 ctcggcgctc agaatgctct cctgggagga ggccgatacg actatctcgt ccgtcacctg
     1501 gggggaccag ccactcctgg tgtcggatgg ggcctgggac ttgaacggct tacttcggtg
     1561 ctgactgctc gctaccctga tctcaccgct tccctggcca tcccccgagc atatgtcgtt
     1621 cactctgacg cagctacagc tgcggccttc gagttgaggc gcatactggc cgacacgggg
     1681 tgggccattg acctggacgc gcgccaggac gggttcggaa agcaactggc acgagcatcc
     1741 aagaggggcg ctcgcatggc gctcatcctc ggtgaggacg agtctgcagc aggcacatgc
     1801 acggtgaagg acctgacctc tggctcacag cagacggtgg cagtgaccga cgtcgccggc
     1861 tgtcttgcag aagctggacg tacctagatt gtgcagacgt ccgactatgt cccctgactc
     1921 acgttggctt tcgtgccttt tcgggtataa tgatgttgcg tcaccctgcg atgcccgtga
     1981 accacttgac gggtttgagc caacacaaat cactcggaag cagatgccct gtggatacga
     2041 gcgcttcgca gtgttcttga agcagggaac tttatgctcc cacctgctta ggcaggttca
     2101 aaagcttcgt caaggcacga ccttgtgggg tggctctttg acagctgtgc gagggcagtg
     2161 ttcttatgca gactgatcga ggattggttg tatcgcgtca tggagatgtt gtcactgtac
     2221 ggctgcagcc gcatgccgag tgtgcgtcgt gctccctgag caagttctct ctgactcagt
     2281 tttgtggagg cagcaaggag agctctgcca ccgtcaaggc aaccgccgcc ccgggaattg
     2341 ctcagggtga tctggtggaa gtctccctcg acgactcgat cactctcaag gcggccgtta
     2401 ttgtctatgg ccttccactt gttgccttcc ttgtgggcgt acttggtggt tacgcactct
     2461 cctcattgat tacgctgagg ggaacgctgt ccacggcagc ccctgtcctg gctggatttc
     2521 tcatgctggt gcctggcgtc gtcctctcgc gcaaagcggc caggcggctg aaccccacgg
     2581 ccactatcgt gggcaaactg gatcaggagg tgaccacgtg ccagtgaatg ccgctgcaat
     2641 ctgggtactg gacattatca ttgcccttgg cctgatcttc atggccgtgg cggctcttgt
     2701 cgtcgggcgc aagcttcagg aattctcaaa gagtctctct gctactatgg ccagcgtggg
     2761 acatcaagcc gatgagctgc agtccgaggc aatcagactg atgcagtcga ctcaggtctc
     2821 cgagcagcat ttcgaccagc tgtcgagaca actggtgaag ctcacggcgt ctgctgacac
     2881 tgtcgtgaaa gcattgcccg cagtggcgtc aggccgagac agcggcctgc tgcccagggt
     2941 catgagcact gtcgtaggtg tggtttcggc gtaccggttc ttcaggaaca tcttcccaag
     3001 gaggaaatca tgagtgaacg caatgactgt ggatcgttca tgggcggatt gctgatggga
     3061 ggcatcattg gagccttggt ggcattggtt ctcgcccccc agagtggaga agagacgcgc
     3121 aagttgctca aggacaaagc tttcgagctg ggcaaggaga tcagcgatca gacagaaacc
     3181 atcaaggaga agggacgtga actcctgaca caggatggaa tcgagattgg agagtccacg
     3241 gatacgaatc cctgagaatc ctgctggcgt ttcacgtgca ggcatcttga ttcgctgcaa
     3301 gttcttggta tactctcgat tgtgttgggg aatcgtccaa cggtaggaca gtagcctctg
     3361 gagctaccga tgatggttcg aatccatctt cctcagccaa gtatggcgct gtagaacagt
     3421 ggttagttcg gggggctctc aatccccaaa caggagttca attctcctcg gcgctaccat
     3481 ctatgcgaac aatgtaacaa cgaggcgggt gacgttatgt cacccgcctc gtttctgtct
     3541 tcctgttccg gctttctgcc tttgtgttca gtgcgagagt cgctgaatga atctccaggc
     3601 gctgtcgaca acgccatcct tgtcatagtc catcgtcact acgtgggctg atctctcgaa
     3661 cacgaccatc tccttgtccg tggaggccac atgctccaag atatgcgggg cacaatcatg
     3721 gggaaccaca tgatcgtgtg tcgaccgcat aacgaggagc ggctgggcga tgagtcccag
     3781 atcccttcgc gtgactccga gcagcttcac catctcatga gcacccgaag ttggcgtcct
     3841 gtcatatgcg ggttcgacga tccctggttc catgatgtcc gacgcgatcg ccggcgtcga
     3901 ggcgaccagg tacttgagga caggcagcag gggcgctcgc ttgtcagtca tgaacacggg
     3961 tgcgttgatg ggaatgacgc ccgcaatctc cggatggtgc tccgcgagat acaaaacaag
     4021 cgttccaccc atcgaaaggc ccgtgacaaa cactctgtca catcggccgg ccagtagctg
     4081 gtatgcctgt tcggcatccc gtatccaatc tgaatagtga accttgttga ggtcctgcca
     4141 cttggtaccg tgaccactca ggcgaggggc ctcaacagat aatccccttg ctgcgagctg
     4201 ctccgcatag taccggatgc taccgactgt ccccgtgaat ccttgcatga cgaggcagcc
     4261 cacacggcca gcccctttcg cgctgaatgg cttgccttct tcgccaatat ccacttggca
     4321 cctccgcttt ggacttgtgc tgtcgtatcg actgcattat gcgcaacccg gactgacgtg
     4381 caactgaaag gactcagcgg agtcctgcag gacgcatccg tcttcggtca gtcgcccagt
     4441 gaactgtctt ctatccctag cgacgagcag gcgtctagat cagtatccgc cctctcgcct
     4501 agcacgcagc gtcgaattcc ggcataggca atcat
//
LOCUS       DATMZD010000167         2137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_35636,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000167 DATMZD010000000
VERSION     DATMZD010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35636"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="VN478_03465"
     CDS             <1..514
                     /locus_tag="VN478_03465"
                     /inference="COORDINATES: protein motif:HMM:NF015716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HWQ21764.1"
                     /translation="AAAQHHILPQLRLQVDLQNRACGLKTLAKLEKDLRKVVGDNLGI
                     ANYRIRGSKKETPPTETALIIARDLARGEVLQTTKDVVVIGSIPEGSRVETMHSCFVF
                     GSIRGTVIAGTQGIQNAIITCLEVAGGASLSISGASAIIHLAPAGMRSHYYVVSAVQG
                     RLNVETRFLE"
     gene            524..1633
                     /locus_tag="VN478_03470"
     CDS             524..1633
                     /locus_tag="VN478_03470"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HWQ21765.1"
                     /translation="MKPHRLPLVFITSVLLLICFGLTTFYVASLAERYTLGHFGSFYS
                     FFQKTLLLLGLSVAIAVMLQFVNYRVFRYLTIPVYVVQTGLLVAVLFIGRGMYGSRRW
                     IPLIGSYGLQPSEIHKLAIILAVAFILTLPEKKLNAGLKILLVMAAVIPSVLLVKKEP
                     DLGSASLLLGISVIMLVHFRLTGRQLVVLGLVLAVAAGGVWLSLRPYQRTRIETFLNP
                     QADPLGDGYNVIQSIVATGSGGMWGRGIKGSTQAPLSFVPVQYADFVFSLVGELFGFA
                     GSALLVVLFIMLLWSTFLVFRSLTDEFARYIVVGIGAMWFLQAAVNMGMTIGLSPVTG
                     IPLPFISYGGTSLLTQVMAASIVVSVYRTRDELRF"
     gene            1635..>2137
                     /locus_tag="VN478_03475"
     CDS             1635..>2137
                     /locus_tag="VN478_03475"
                     /inference="COORDINATES: protein motif:HMM:NF040068.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HWQ21766.1"
                     /translation="MDMHSLERFEGPSRYAGNELNAVHKEWKGRFRVALSYPDLYEVG
                     MSSYGIQILYHVLNALDDVVVERVFAPFHDLEAWLREHKVPLFSLESRQPLGAFDVVA
                     FSLGTELTYTNVLNMLDLAGLSPRSEDRTEWPIVIAGGSCTFNPEPMSAFIDAFGIGD
                     GEELFPE"
BASE COUNT          394 a          659 c          606 g          478 t
ORIGIN      
        1 ggcggccgcc cagcatcaca tcctccccca actgagactt caggtcgacc ttcagaaccg
       61 ggcatgcggc ctcaagacgc tggcaaagct cgagaaggac ctgcggaagg tcgtgggcga
      121 caatcttggc attgccaact atcgcatacg aggttcgaag aaggagacgc ctccaacaga
      181 gacggccttg atcatcgccc gtgacctggc ccggggcgag gtcctccaga caacgaagga
      241 cgtcgtcgtt atcggctcga ttcccgaggg atcgcgagtt gaaacgatgc actcgtgttt
      301 cgtgttcggc tccatcaggg gaacagtcat cgcaggcact caggggattc aaaacgctat
      361 catcacatgc ctcgaggttg ccggtggcgc ttcgctttcc atctcgggcg caagcgccat
      421 catccacctg gcgccggctg ggatgcgaag tcactactat gtggtgagcg ccgttcaggg
      481 tcggctcaac gtcgagacgc gcttcctgga gtagagacat cccgtgaagc cacatcgcct
      541 gccgctggtc ttcatcacta gcgtcctcct cctcatctgc tttggcctga cgacgttcta
      601 tgttgccagc ctcgctgagc ggtacacgct tggacacttc ggcagcttct actccttctt
      661 ccagaagacg cttctgctgc tcggtctcag tgtggcaatt gcagtcatgc tccaattcgt
      721 gaactacagg gtcttcaggt acctgacgat ccccgtctac gtcgtacaga cagggctgct
      781 ggtcgccgtt ctgttcatcg gccgcggcat gtacggttca cgccgctgga tccccctgat
      841 aggatcatat ggactccagc cgagcgagat acacaagctg gccatcatcc ttgcggtcgc
      901 gttcatcctg acgctcccgg agaagaagct caacgcaggg ctgaagatac tccttgtcat
      961 ggcggccgtc ataccgtctg tcctcctcgt caagaaggag ccggacctgg ggtctgcgag
     1021 tctgctcctc gggatctcgg tcatcatgct ggttcacttt cgactgaccg gccgtcagct
     1081 ggtggtcctc gggctcgttc tggcagttgc ggccggtggt gtctggctca gcctgaggcc
     1141 gtatcagcgt acacggatcg aaacttttct gaatccacag gcagacccgc taggcgacgg
     1201 ctataatgtc atccagtcca tcgtagccac cgggtctggc ggcatgtggg ggcgaggcat
     1261 caagggtagc acccaggctc cattatcgtt tgtgcccgtc caatacgccg acttcgtctt
     1321 ctctcttgtg ggcgaactgt tcgggttcgc cggcagtgcg ctgctggttg tcctgttcat
     1381 catgctcctg tggtcgacgt tcctcgtctt ccgatctctg accgacgaat tcgcccgcta
     1441 catcgtcgtg ggcataggag ccatgtggtt cctgcaagct gccgtgaaca tgggcatgac
     1501 tatcgggctt tcgcccgtca ccggcattcc actgccgttc ataagctatg gcggaacgtc
     1561 cctgcttacg caggtgatgg ccgcctccat cgtcgtgagc gtctaccgca cacgcgacga
     1621 attgaggttc taacatggat atgcactctc tcgaacgttt cgaaggcccg tcgcgatatg
     1681 caggaaacga gctcaacgca gtccacaagg agtggaaagg ccgcttccgc gttgcgctca
     1741 gctacccgga cctctatgag gtcggcatgt catcctacgg catccagatc ctctatcacg
     1801 tcctcaacgc actcgatgac gtcgtcgtgg aacgggtgtt tgcaccattc cacgatctcg
     1861 aggcgtggct cagggaacac aaggttccgc tgttctcact cgagagtcgt cagcctctgg
     1921 gcgcctttga cgtcgttgcg ttcagcctcg gtaccgaact gacctatacg aacgtgctca
     1981 acatgcttga cctggccggg ctctcaccac ggtcggagga tcggactgag tggccgatcg
     2041 tcatagcggg cggcagctgc acgttcaacc cggaacccat gtccgccttc atcgacgcgt
     2101 ttggcatcgg cgacggagag gaactcttcc cggagat
//
LOCUS       DATMZD010000168         4383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_4845,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000168 DATMZD010000000
VERSION     DATMZD010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4383
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4845"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /locus_tag="VN478_03480"
     CDS             <1..967
                     /locus_tag="VN478_03480"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWQ21767.1"
                     /translation="AADLTKGLLTFGRRAMVSAAPLDMSEVIDTTLTILNQSLPATIK
                     IVRDVEEPAWSVLADHSQIAQVILNLAVNARDAMQGRGTLTIRLRNAYIDEQYVRMHP
                     LSRTGDFVRLSLADTGPGIPDEILKHLFEPFHTTKPIGSGTGLGLSIVYGAVTQANGW
                     VTAESSPGAGTVFNVFLPRCLDKPTTPVAAVRVPASICTGTLLVVEDEPVVCAVAQAL
                     LTRGGCSVLTAGDGAAALGVFRERADTIGLILLDMTMPGMTTDEIIRSIRSVDSRVPI
                     LLTSGYTSESTVKHMLDEGIVQGFLAKPYDPGQLMDSVQMLFRRE"
     gene            1174..3561
                     /locus_tag="VN478_03485"
     CDS             1174..3561
                     /locus_tag="VN478_03485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWQ21768.1"
                     /translation="MSPVDTTERFVQSGDILIVDDAGADFQLLETLLTHAGYQVHVAT
                     AVDIVMRSVEVRLPDLILLKADMPDVDCRDLCYRLRVHAETVDIPIICFGASATTGQK
                     AGSLAAGAVDFITEPFDEAEVLGTIAVHLSLNEALRDLKRQNARLQEEVARGRQAEQA
                     LAASHSALLEAQTIAHIGDWSLDAATRTYVWSDEMYAIHGMSQGVPVPHDVYAGLIHP
                     DDRQRVLEAMRSGMTGKQQEFSVDYRIVRPDGTERLVSLTGKTLLDSTGTVSGVRGTM
                     QDITDRTRVEAGLRESERRYHLLFDNMVNGFALHEMLYDERGNPVDYRFLEANQAFER
                     LTGVPVASIVGHTVLEVLPDTEPRWIQTYAGVVQTGTPALFEEYSGAIGKWFETRAFR
                     VAENQFATIFADVTERKRAEQELRDSENRFRAVFEQAAVGVALLNTKSGQFVRINQRY
                     CDFLGYTMEEMLQKTFADVTFAEDVQQNVEGNAQLMDGTRSEFSYEKRYLRKDGGVVW
                     GSLAISPLWKPGEKPATYFHVAIVQDITDRKRAEEEKEAMQAQLLQSKKLEAVGQLAG
                     GVAHNFNNLLTGILGNIDLARSDLDAAHPASSSLDTARAAALRAASLARQLSSFARNA
                     MVLPVVQSANSAVQTALELVCSSLPSSIGVIRDLTPDAWDVEVDSSQLTQVLLDLTNN
                     AREAMDDRGSISVRTRNITVDAAYVAATPFARTGEFVVISIADTGPGIDPAIIPRLFE
                     PFATTKLFGRGMGLPTALGALRQAGGWVAVCSEPGAGTRIDLFLPRHVTGPSPSA"
     gene            complement(3637..>4383)
                     /locus_tag="VN478_03490"
     CDS             complement(3637..>4383)
                     /locus_tag="VN478_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006287596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWQ21769.1"
                     /translation="DVSFDGISFGYSNDKLLMDDLSIEAPCGETIAIVGPTGAGKTTL
                     VNLLMRFYDIQGGAITFDGVETRKIGRGSLRTMFGMVLQDTWLFNGTIRENIAYGRDD
                     ATEEEIVAAAKAAHADHFIRSLPEGYNTVLNEEASNISAGQKQLLTIARVVLANPAVL
                     ILDEATSSVDTRTEVRIQKAMAQLMTGRTSFVIAHRLSTIRDAHHILVMDHGAIVEQG
                     THQELLAKHGFYADLYNSQFTGALVTDGPA"
BASE COUNT          860 a         1338 c         1381 g          804 t
ORIGIN      
        1 ggccgccgat ctcacgaagg gactgcttac gttcggccgc agggccatgg tctcagcagc
       61 gcccctcgac atgtcagagg tcatcgacac gacgctgacg atcctgaatc aatccctccc
      121 tgcaaccatc aagatcgtcc gcgacgtgga ggaaccggcc tggagcgtcc tggcagacca
      181 ttcgcagatc gcccaggtga tcctcaacct cgctgtgaat gcgcgcgatg ccatgcaggg
      241 aaggggaaca ctcacgatcc gtctcaggaa tgcatacatt gacgagcagt atgtccgcat
      301 gcacccgttg tcgcgaacag gggacttcgt ccgtctgagt ctggcagaca caggtcccgg
      361 catcccggac gagatcctca agcacctctt cgagccattt cacacgacca agccaatagg
      421 gtcgggaacg ggcctgggcc tgtccattgt gtatggagca gtcacgcaag ctaatggatg
      481 ggtgacggca gagtcatctc caggcgccgg gaccgtgttc aacgtattcc tgccgcgctg
      541 tctcgacaag cccactactc ccgtcgcggc agtccgcgtt ccggcgagca tctgcacggg
      601 gacactcctg gtcgtggaag acgaaccggt cgtatgtgcc gtcgcccagg cactgctgac
      661 gcgcggaggc tgcagcgtct tgacggccgg agacggcgct gccgcattgg gcgtgttccg
      721 cgaacgtgct gacaccatcg gcctcatcct gctcgacatg accatgccgg ggatgacgac
      781 cgacgaaatc atacgaagca tccgctccgt cgattcgcgc gtgccgatcc tgctgacttc
      841 cgggtacact tcggagtcta cggtcaagca catgctcgac gaaggcatcg tacagggatt
      901 ccttgccaag ccctacgacc cgggacaact gatggacagc gtgcaaatgc tgttccgccg
      961 cgagtagcag gtgcctgcat gtatcagacg gttgagcgtg gcggccgtag aacagctgtg
     1021 gcatgaggcc cagatgaaca gaggaagcca gggtcaggtt cgtgcgggag gatgcgtaac
     1081 agctgtggct ggtcgcctgc acaccaggga ctgtagtctg ctacaccgtg ggccgtccga
     1141 agccgtcgac tcggaggcga aggagagctt gcgatgagtc ccgtcgatac gaccgaacgg
     1201 ttcgttcaga gtggggacat cctgatcgtc gacgatgcag gcgctgactt ccagcttctg
     1261 gagaccctgc tgactcacgc cggctatcaa gtgcacgtcg ccactgccgt cgatatcgtt
     1321 atgcgcagcg tcgaagtccg gctgccggac ctcatcctcc tgaaggccga catgccggat
     1381 gtagactgcc gcgatctgtg ctaccggctc agggtccacg cggagactgt cgacataccc
     1441 atcatctgtt tcggtgcgtc agcaacgact ggacagaagg ccgggagcct cgctgcagga
     1501 gctgtcgact tcatcactga gccattcgac gaggccgaag tccttggcac gatcgccgtg
     1561 catctgtccc tgaacgaagc gctgcgggat ctgaaacgcc agaacgcccg gctccaggaa
     1621 gaagttgctc gaggcagaca ggcggagcag gcactggcag ccagtcatag tgccttgctt
     1681 gaggcacaga caatcgccca catcggcgac tggagtctcg acgcagcgac gcgcacgtat
     1741 gtatggtcag acgagatgta tgccatccat ggcatgtctc aaggcgtgcc ggtcccgcac
     1801 gacgtctacg caggactcat ccaccctgac gaccgtcagc gtgtcctcga ggccatgcgg
     1861 tcgggcatga caggcaagca gcaggagttt agcgtcgact accgcatcgt gcggcccgac
     1921 ggaactgagc ggcttgtctc cctgacgggc aagacgctcc tggacagcac cggcactgtg
     1981 tcgggggtac gcggcactat gcaggacatc acggaccgaa cgcgagtaga ggcgggcctg
     2041 cgggagagcg agcgccggta tcacctgttg ttcgacaaca tggtgaacgg ctttgccttg
     2101 cacgaaatgc tgtacgacga acgcggaaac cccgtggact accggttcct cgaggccaac
     2161 caggcgttcg agcgtctgac aggagtaccg gtcgcatcca tcgtgggtca tacggtcctg
     2221 gaggtccttc cggacacgga gccccgctgg atccagacct atgccggggt cgttcagacc
     2281 gggactccag cgctcttcga ggagtactcc ggcgccatcg gcaagtggtt tgagacacgg
     2341 gccttccgag tagccgagaa ccagttcgcg acgatcttcg ccgacgtcac ggagcgtaaa
     2401 cgcgccgagc aggagctgcg ggacagtgag aaccggttcc gtgccgtctt cgaacaagct
     2461 gcggtcggcg ttgcgctgct gaatacgaaa tccggacagt ttgtccgcat caaccagcga
     2521 tactgtgact tcctgggcta cacgatggag gagatgctgc agaagacctt cgcagacgtc
     2581 acctttgctg aagatgtcca gcagaacgtc gaaggaaacg cacagctcat ggatggcacg
     2641 agaagcgagt tctcatacga gaagcgctac ctgcgcaagg atgggggcgt agtctggggc
     2701 agtcttgcga tctccccgct atggaagccc ggcgagaagc ctgctacgta cttccacgta
     2761 gccatagtcc aggatatcac agatcgcaag cgtgccgaag aagagaagga agccatgcag
     2821 gcgcagcttc tgcaatccaa gaagctggaa gccgtcggtc agctggccgg cggcgtcgcg
     2881 cataacttca acaatctgct gaccggcatc ctgggaaata tcgacctcgc gcgctctgac
     2941 ctggacgccg cacatccggc ctcgtccagc ctggacactg caagggcggc tgccctcagg
     3001 gccgccagcc tggccagaca actgtcatcg ttcgcccgca acgcgatggt cctgccggtg
     3061 gtgcaaagtg ccaacagcgc agtccagaca gccctggaac ttgtctgctc gtcgcttccc
     3121 tcctcgatcg gcgtcatacg ggacctcacg ccggacgcgt gggacgtcga ggtcgactcg
     3181 tcacagttga cgcaggtcct gctggatctg acgaacaatg cgcgtgaagc catggacgac
     3241 aggggatcga tcagtgtccg caccaggaac atcaccgtgg atgccgcata cgtggcggct
     3301 actccctttg cccgaacggg cgagtttgtc gtcatctcaa ttgccgacac tggaccgggc
     3361 atcgaccccg ccataatccc acggctcttc gagcccttcg cgacgaccaa gctgtttggc
     3421 cggggcatgg gacttcccac ggcactggga gccctcaggc aggctggagg atgggtggcc
     3481 gtatgctcag aacccggcgc aggaaccagg atcgacctct tcctgccacg ccacgtcacg
     3541 gggccgtctc catctgcctg accccgaagg atccgagaaa gaccccggct atatggccgg
     3601 ggtcagtgat ggatgcggac ttggcccgct gtcagttcag gcgggtccgt cggtgacgag
     3661 cgcccccgtg aactgactgt tgtacaggtc ggcgtagaac ccatgcttcg caagcagttc
     3721 ctggtgcgtg ccctgctcga cgatggcgcc gtggtccatg accaggatgt ggtgcgcgtc
     3781 gcggatcgtc gagagacggt gggcgatgac gaagctggtc ctgccggtca tgagctgtgc
     3841 catcgccttc tggatgcgta cctcggtccg ggtatcgacg ctgctggtgg cctcgtcgag
     3901 gatcaggacg gccgggttgg ccagcacgac acgcgcgatg gtcagcagct gcttctgacc
     3961 ggcggagatg ttggaggctt cctcattgag gacggtgttg tacccttcgg gcaggctccg
     4021 gatgaagtga tcggcgtgcg ccgccttggc agcggccacg atctcctctt cggtcgcatc
     4081 gtcgcgcccg taggcgatgt tctcgcggat cgtgccgttg aacagccatg tgtcctggag
     4141 gaccatgccg aacatggtgc gcaggctgcc gcggccgatc tttcgtgtct cgacgccgtc
     4201 gaaggtaatg gcgccgccct ggatatcgta gaaacgcatg agcaggttga cgagggtcgt
     4261 cttgccagcg ccggtcgggc cgactatcgc aatggtctct ccgcagggcg cctcgattga
     4321 caggtcgtcc atgaggagct tgtcgttgga atagccgaag cttatgccgt cgaacgacac
     4381 gtc
//
LOCUS       DATMZD010000169         4427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_55373,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000169 DATMZD010000000
VERSION     DATMZD010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4427
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55373"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="VN478_03495"
     CDS             complement(<1..497)
                     /locus_tag="VN478_03495"
                     /inference="COORDINATES: protein motif:HMM:NF024211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HWQ21770.1"
                     /translation="MKTTLSTGHRRQSVRKALIISSFLLFPVTFYYLSPYLIIMGAGE
                     RTASGSMVVFGVMALAAIVLGRLFCGWICPAGGLAESLFAVQNRRANNRRNWTRWLVW
                     IPWMTVVLLVTLRAGGIQHIDFLYQTDRGVSLTTAGGMGPYIIFYAVLALVFVLSVAA
                     GRRGF"
     gene            complement(609..1319)
                     /locus_tag="VN478_03500"
     CDS             complement(609..1319)
                     /locus_tag="VN478_03500"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HWQ21771.1"
                     /translation="MRRVVSLIFVPAVLFLSVFVLSAVLLEHHAPVYLSLPIAILVVL
                     VPFELSVLLAANRRQYGTTGLMVAFKRHEPMPVWRIVLEGMILFGWAGLVYAVLQPLE
                     NSILLGSVFRFVPGFLRSTTFATELAGFPKAVIVLTCTTNLIVTGVIGPIVEELYFRG
                     YLMSRMEYLGKWAPLVVSVLFSAYHLFTPWENVTRIVVFLPLHYTVWKERNLYIGMVA
                     HCACNLAVGASLFSLAMK"
     gene            complement(1446..3611)
                     /locus_tag="VN478_03505"
     CDS             complement(1446..3611)
                     /locus_tag="VN478_03505"
                     /inference="COORDINATES: protein motif:HMM:NF028237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HWQ21772.1"
                     /translation="MTPYTLPLLAAATIAAAVSVLAWRRRPSRGAVAFSALMLLVVVN
                     ILVSLALLGSTSLDSFLFWVKMSFAGGGLGVAWLVFTLRYDGRSLRDVTRIGVLVAVE
                     PAAVFVLAWFNDLHHLLWTQMSVAPVTIPGSLLHVDTSFGPLYWVWSACQYTLLLAGV
                     VVLMRNMYHARSAYRGQTIAVLVAMAVPWAANALFLIGYTFVTGVDLTPFAFALSGVA
                     LFFALYRFRFLDLVPIPRSLVLEASPNGVLVLDARGRIVDVNTAAEGILGCSAQQVLG
                     IEGVLVLPALSSAAPDADIELAFVQNGVERSYDLHVAPLRKHMGTLVQLTDVTDRKRM
                     AARLLQAQKMDALGLMAGGIAHDFNNLLTGILGNISIVRDLADRSEPIAEPLAQAEKA
                     ARRAAGLTHNLLTFSRSGSPSPTVVDLNESVDLTLQSIAGLIPSTMRIVRDFAPGVWN
                     VLVDQVQFGQLAINLVLNARDAMDGMGTLTVRTGNVDIDEPFVQAHPEARAGEHVLLQ
                     FTDTGRGMSDAVRQHLFEPFFTTKPVGQGTGLGLAVVYGAVQQARGWIIVDTAAGEGT
                     VFSTYLPRCREPAQDPAVQETRVQRVPANKTIMVVDDEEMILLLTRRMLERAGYKVIA
                     APDGPSAIASFQQDPAAVDLVLLDMTMPHMTGDQVLWELRRLGCKAPILISSGYSLGD
                     SIQQLVGAPGGADGFLPKPYSIQELSDAVSAALESVYKT"
     gene            4070..>4427
                     /locus_tag="VN478_03510"
     CDS             4070..>4427
                     /locus_tag="VN478_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21773.1"
                     /translation="MSRGFLPLTVGAVLACACGTVGAAVAVAGSLAAGCSGLRPRLWD
                     WVAFGVVATGEDAACGTVGAATAVAGSLAAGCSGLRPRLWDWAPLGVVVEGDWDCPGE
                     RSPRGVTAGVCWGFGSE"
BASE COUNT          912 a         1286 c         1384 g          845 t
ORIGIN      
        1 cagaatccac gcctgcctgc cgcgaccgac agcacgaaga cgagcgcaag gacagcgtaa
       61 aagatgatgt agggtcccat gccacctgca gtggtgaggg aaaccccgcg gtccgtctgg
      121 tacaggaagt caatgtgctg aatgccgccc gcccgaagcg tcaccagaag gacaactgtc
      181 atccagggga tccagacaag ccagcgcgtc cagttccgcc ggttgttcgc cctcctgttc
      241 tgcacggcaa acagggattc ggccagtccc ccggcgggac atatccaacc gcagaacagg
      301 cgtcccagca cgatcgccgc tagagccatc acgccgaaca ccaccatgct tcccgacgct
      361 gtccgttcac cggcgcccat gatgatgagg taaggtgaaa ggtagtagaa ggtgacaggg
      421 aagagcagaa aggagctgat gatcagtgct ttacggacgc tctgacgcct gtgtccggtc
      481 gaaagtgttg ttttcatgtg gtgcctccgg cgagcagtat agcgacagca tgtcgccatg
      541 caacgccggc attgtattgt cctgattaga tgtggactct ctcctccccg tcaaagaaga
      601 gacactgact atttcatggc gagtgagaac aaggatgctc ctactgccag attgcaggca
      661 caatgagcta ccatgcctat gtacaggttc ctttccttcc atacggtgta gtgaagcggg
      721 aggaaaacga ctattcttgt cacgttttcc cacggagtga agaggtgata ggcagagaac
      781 aagactgaaa caacgagagg tgcccatttg ccaaggtact ccatcctcga catcaggtag
      841 cccctgaaat acagttcctc tactatgggg ccaatcacgc cagtgacgat cagattcgtt
      901 gtgcaagtca gaacgatcac agccttcgga aagcctgcaa gttcagtggc gaatgttgtg
      961 gaccgcagaa agcccggaac gaacctgaag acagagccta gcaagatgga gttctcaaga
     1021 ggctgcaaga ctgcatacac caggcctgcc cacccgaaca ggatcattcc ttccagaacg
     1081 attctccaga ctggcattgg ctcatgtcgc ttgaatgcca ccatcaagcc cgttgtgcca
     1141 tattgtctcc tgttggcggc aagcaggaca gagagttcaa atggcaccag cacgaccagg
     1201 atggcaatcg gcaagctcag atacacggga gcatggtgct cgagcagcac tgcagagaga
     1261 acaaagactg acaagaacag tacagccggc acgaagatca gtgagacgac cctcctcatg
     1321 ctcaactgtc cttcagaaca tgctgttgtc ataggaccct ccatcatctt ctccattgcc
     1381 tcagtcccct ggaacaccag caatgggctg cagtacgtcc cgactctctg tgagagggtg
     1441 gaaattcagg tcttgtagac gctctccagt gccgcactga ctgcgtctga cagctcctgg
     1501 atgctatacg gttttggcag gaacccgtca gcgccacctg gggcaccgac cagctgctgg
     1561 atactatcgc ccagcgagta cccggatgag atgagaatgg gagccttgca gcccagccgc
     1621 ctcagttccc agagtacctg gtctcccgtc atgtggggca tggtcatatc gagaaggacg
     1681 aggtccacag cggccgggtc ctgctggaag gaagcgatgg ctgacgggcc gtcaggagct
     1741 gctatcacct tgtagccggc gcgttccagc atgcgcctgg tgagcaacag gatcatctcc
     1801 tcgtcgtcga cgaccatgat cgtcttgttt gcgggtactc gctgcacgcg cgtttcctgc
     1861 accgcggggt cctgcgcagg ttccctgcac cgaggcagat acgtcgagaa cacagtgccc
     1921 tctcctgccg ccgtatccac gatgatccag cccctggcct gctggacagc gccgtagacg
     1981 actgcgaggc ccagcccggt cccctgtccg actggcttgg ttgtgaagaa cggttcaaag
     2041 agatgctgtc gcaccgcatc actcatacct ctgccggtgt ctgtgaactg caggagaacg
     2101 tgttcgcctg cccgggcctc gggatgtgcc tggacaaatg gctcgtcgat gtcgacattg
     2161 ccggtccgga cggtcagggt ccccatgccg tccatggcgt cccgcgcgtt caggacgaga
     2221 ttgatggcaa gttgtccaaa ctgaacctgg tcgacgagca cattccacac tcctggagca
     2281 aagtcccgga ctatcctcat ggtggacggg atgagtccgg cgatcgactg caaggtgagg
     2341 tcgaccgact cgttgagatc gacgaccgtc ggcgatggac ttccacttcg gctgaacgtc
     2401 agcaggttat gtgtgagccc tgcagctcgc cgcgcagcct tctcagcctg tgccagcggc
     2461 tccgcgatgg gttctgaccg atcggccaga tcgcggacaa tgctgatgtt gcccaggatg
     2521 ccggtcagca ggttgttgaa gtcgtgagca atgccgcccg ccatgaggcc gagggcatcc
     2581 atcttctgcg cctgaagcag gcgagcggcc atgcgcttgc ggtcggtgac gtccgtcagc
     2641 tggacaagcg tccccatgtg ctttcgcaga ggagcgacgt ggagatcgta ggagcgctcc
     2701 acgccgtttt ggacgaaggc cagctcgatg tcggcgtctg gtgcggctga cgaaagggca
     2761 ggaaggacca gtaccccttc gatacccagc acctgttggg cggagcatcc aaggattccc
     2821 tcggctgcag tgttgacgtc cacgatgcgg cctcgggcgt caagcacgag caccccgttt
     2881 gggctggcct cgaggacgag cgagcgggga atcgggacga ggtcgaggaa gcggaagcga
     2941 tagagtgcga agaagagagc gacgccgctc agggcgaacg cgaacggagt cagatcgacg
     3001 ccggtgacga acgtgtagcc gatcaggaac agcgcgttcg cggcccacgg tacagccatt
     3061 gcgaccagga cggcgatggt ctggccacgg taggcggaac gagcgtggta catgttcctc
     3121 atgaggacca cgacgccggc cagcaggagg gtatactgac atgcactcca gacccagtag
     3181 agcggaccaa agctcgtgtc gacatggagg agcgaaccgg ggatagtgac cggcgcgacg
     3241 ctcatctgag tccaaagcag gtggtgcagg tcgttgaacc aggccagcac gaatactgct
     3301 gccggctcga ccgccacgag tacgccgatg cgcgtgacgt cacgcagaga tcttccgtcg
     3361 tacctgagcg tgaagactag ccaggcgacc ccaaggcctc cgccggcaaa ggacattttc
     3421 acccagaaca ggaaggaatc caggctcgta ctgccgagga gagccagcga gacgaggatg
     3481 ttcacgacaa ccagcagcat gagagccgag aaggcaacgg cgccgcgtga aggcctgcgt
     3541 cgccaggcaa ggactgagac ggcagccgcg atggtcgctg cggccagcag tggcagcgtg
     3601 tacggtgtca tttgtgtgtc caagcgtctt ccgtcccttt catgtgcacg actccagtgg
     3661 ggcacgtccc ggcactggtc ttcagcgcgg ctgcctgcac atgtagtcta gccgcagatg
     3721 caatgcatgt caaagaacga gttgggtcag agatgagtag cagcgccccg ggaccacagt
     3781 cccggggcgc tcaatctggc gattcagtga gttctacctg gtaccatccg caccaggctt
     3841 gggagccttc tggttccgct gggacttggc tggagtcctg gcagtctgtg cagccggttt
     3901 cgattcaggt ttctgagatt caaccttgag ggcctctctg ccactcttga ccacaggagc
     3961 cttgccagcc gcttccggct ttctgcctgc ctgcctggcc ggtccatgtt gttgctgcgc
     4021 tgctgcaggt ccctgcctgt gggtctgggc gttcttcgtg ggagagctcg tgtcgagagg
     4081 cttccttcca ttgaccgtcg gtgctgttct ggcctgtgcc tgcgggacag ttggagctgc
     4141 tgtggcagtt gccggctcac tggctgcagg atgcagcggc ctgcggccgc gcctctggga
     4201 ctgggtcgca tttggcgtgg tcgcgacggg tgaagatgct gcctgcggga cagttggagc
     4261 cgctacggca gttgccggtt cactggctgc aggatgcagc ggcctgcggc cgcgcctgtg
     4321 ggactgggcg ccgttgggcg tggtcgtgga gggagactgg gactgccctg gtgaacgttc
     4381 ccccagggga gtgacggcag gtgtctgctg gggcttcggc tctgagg
//
LOCUS       DATMZD010000170         2463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_35726,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000170 DATMZD010000000
VERSION     DATMZD010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35726"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="VN478_03515"
     CDS             complement(<1..583)
                     /locus_tag="VN478_03515"
                     /inference="COORDINATES: protein motif:HMM:NF013665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HWQ21774.1"
                     /translation="MEPMTHLSDIAAAVKGLSRKRMAVAYGEDTHTLQAVQRAVREGI
                     VDAIVVGGAAKIAEVAAAGGVDMGLFEVMDVQGEMPAVKAAVKLVREKKADILMKGLA
                     HTDNYMRGILDKENGLLPEGGLLSHVSVFEAPNYPRLMIMSDVAVVPLPSLQDKVRIT
                     KLAIAVAHSLRIDVPKVAFLSASESVLKFASSQE"
     gene            complement(594..1496)
                     /locus_tag="VN478_03520"
     CDS             complement(594..1496)
                     /locus_tag="VN478_03520"
                     /inference="COORDINATES: protein motif:HMM:NF006045.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HWQ21775.1"
                     /translation="MEIRTFDQLLDEAKKRGRKKLAVAFPEEVSLHAALLAYEAGIAA
                     PILVGIPGKIRDLLQEAGADVDSFQIEEAASPAEACALAVSLGRDDKADVILKGGAQT
                     AQMLHAVLDKDNGIRASKVLSECSLYEDTRVRHSLLAVVDGGILIAPTLEEKVEAIRN
                     TVNVYHKLGYENPKVAVLAAVETVTDKMPCTEDAAIIARMSERGQIKGCVVDGPLALD
                     NAISERAATIKGLSGAVAGNADILIVPDIEGGNLLGKSMTYFAGFTSAHVTIGARKPV
                     LVPSRADDGRTKLMSVALGIVAYY"
     gene            complement(1507..>2463)
                     /gene="buk"
                     /locus_tag="VN478_03525"
     CDS             complement(1507..>2463)
                     /gene="buk"
                     /locus_tag="VN478_03525"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002834.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HWQ21776.1"
                     /translation="VAQFPHVADQLAMRTQAVAAFLERHHAAVSSLEAVMARGGLLHP
                     VAAGVYAVNDRMLEDSREMRVAEHASYLGALIAHELAAGTSIPCYIADPVSVDEFDDV
                     ARISGLKELPRYSFVHALNIRKVAYKTAERHGKSLSDMNMIVAHLGGGISIAALRRGR
                     MVDENDANNGGPYAPERAGCVPACSLVRLCFAPGATERGMRRQLTQQGGLVSHLGTSD
                     AREVRRRVDAKDAQAGLVYEGMAYQIAKHIGGMATVLEGDVDVIVLTGGLAHDDLLMQ
                     WISDRVGFIAPVEIVPGENELEALNEAFVRLRQGLETPKAYD"
BASE COUNT          453 a          827 c          698 g          485 t
ORIGIN      
        1 cctcctggct gctcgcgaac ttcagcacgc tctcgcttgc cgaaaggaag gccaccttcg
       61 gaacgtcgat gcgaagactg tgcgcaacgg ctatcgccag cttcgtgatc cggaccttgt
      121 cctgaagcga aggcagcggg acgaccgcga cgtcggacat gatcatcagg cgcgggtagt
      181 tcggcgcttc gaataccgaa acatgtgaca gcagaccacc ttcaggcaac aggccgttct
      241 ccttgtcgag gatgccccgc atgtagttgt cggtgtgggc gagtcccttc atgagaatgt
      301 cagccttctt ctcgcggacc agtttgacgg cagccttgac tgcaggcatc tcgccctgga
      361 cgtccatgac ctcgaacagt cccatgtcga ctccgccggc cgccgctacc tcggcgatct
      421 tcgctgctcc gcccacgacg atggcatcga cgatgccttc cctgaccgct cgctgcactg
      481 cctgcagcgt atgcgtgtcc tcgccgtacg ccacagccat ccgcttgcgc gacaaaccct
      541 ttactgcagc cgcgatatcc gacaagtgcg tcattggttc cataccagtc ctcctagtag
      601 taggccacaa tgccgagggc cacggacata agcttggtac gcccatcgtc cgccctggaa
      661 ggcacgagca cgggcttccg ggctccgatg gtgacatggg cagacgtgaa gcccgcgaag
      721 taggtcatgg acttcccaag caggttgccg ccctcgatgt ccgggacaat cagaatgtcg
      781 gcattcccgg cgaccgcacc gctgagtccc ttgatggtcg cagcgcgctc tgagatggcg
      841 ttgtccagtg ccagcggtcc atctacgacg catcccttga tctgtccgcg ctcggacatc
      901 cgcgcgataa tcgctgcgtc ctccgtgcac ggcatcttgt ccgtcacggt ctcgacggcc
      961 gccagtactg cgacctttgg attctcgtag cccagcttgt ggtacacgtt cacggtgttc
     1021 ctgatggcct ccaccttctc ctcgagcgtt ggcgcgatca gaatgccacc gtcgacgacg
     1081 gcgaggagcg aatgtcgtac tcgcgtgtcc tcgtagagcg aacactcgga gagcacttta
     1141 ctggcacgga tgccattgtc cttgtccagg actgcgtgca gcatctgcgc ggtctgagct
     1201 cctcccttga ggatgacgtc cgccttgtca tcccggccga gggaaacggc aagagcacat
     1261 gcctccgctg gtgatgcggc ctcctcgatc tggaatgagt cgacatcagc cccggcctcc
     1321 tgcagcaggt cccggatctt cccggggatg cccacgagga tcggcgctgc aataccagcc
     1381 tcgtacgcaa gcagggcggc atgcagcgac acttcttccg ggaatgcaac tgccagcttc
     1441 ttccgtcctc gcttctttgc ttcatccagc agctggtcaa acgtcctgat ctccatgaat
     1501 cctcctctag tcgtacgcct tcggcgtttc cagcccctgc ctcaggcgaa cgaatgcctc
     1561 gttgagtgcc tccagctcat tctctcccgg cacgatctcg acaggagcga tgaacccaac
     1621 acgatcgctg atccactgca taagcaggtc gtcgtgcgcg agtccgccgg tcagcacgat
     1681 gacatctacg tcaccctcaa ggacagtggc cataccgcca atgtgcttcg cgatctgata
     1741 tgccatgcct tcatagacaa ggcccgcctg agcgtctttc gcatcgacac gccgacgcac
     1801 ctctcgcgca tcgctagtcc caagatgcga gacgagccct ccctgctgag tcagctgccg
     1861 ccgcatgccc cgttcggttg cccccggggc aaagcacaac cgcaccaggg aacatgcggg
     1921 cacgcatcct gctcgctccg gcgcgtacgg ccccccgttg ttcgcgtcgt tctcatccac
     1981 catacggcca cggcgaagcg cggcgatgga aatacctccg cccagatgag cgacgatcat
     2041 gttcatgtca ctcaggctct tgccatgccg ctctgcagtc ttgtaggcga ccttgcggat
     2101 gttgagggcg tgcacaaacg agtatcgcgg cagttccttg agccctgaga tgcgcgcgac
     2161 atcatcgaat tcgtccaccg acacagggtc ggcgatgtag caggggatac tcgtgccggc
     2221 agccagctcg tgggcgatga gagcgcccag gtaggatgcg tgctcggcaa ctcgcatctc
     2281 gcgcgagtcc tcaagcatgc gatcgttgac agcgtaaacg ccggctgcaa ctggatgcag
     2341 cagtccaccg cgagccatga cagcctcgag ggaggacacc gccgcgtggt gtcgctccag
     2401 gaaggccgcg acagcctgcg tgcgcatcgc cagctgatcc gcgacatgcg ggaactgtgc
     2461 gac
//
LOCUS       DATMZD010000171         2611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_95577,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000171 DATMZD010000000
VERSION     DATMZD010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95577"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..130
                     /locus_tag="VN478_03530"
     CDS             8..130
                     /locus_tag="VN478_03530"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HWQ21777.1"
                     /translation="MDYDERVRLDMDYITHWSLWLDLRILASTIPAVLRKKGAR"
     gene            354..2012
                     /gene="murJ"
                     /locus_tag="VN478_03535"
     CDS             354..2012
                     /gene="murJ"
                     /locus_tag="VN478_03535"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HWQ21778.1"
                     /translation="MTEDSGGRSTRDSAMSHGRGLLRRQSVAQAAVFLSVVTLFSKFI
                     GFARDAIVAHNWGATGLTDAFLIGMMVPSVLLGIVSTGLSTIIVPWYIAHRKDDPARA
                     RELVNQVTFVWGMAFLAASLLVVAFAPQLVHLFAPSFTDFRYDVAVKVTRLLVPMGFF
                     NVMTGLVTGLSQAEGQFLIPLLSNVFGNVLMVGCLIFFATPLGIQSYSLGQTVMSVIG
                     FAPVLWFLVRRRGFFRHLDMRHVDWRAIGSFAALLVPLVISGGGGSMNTVVDRWFASH
                     LSQGSGSALDYSNRLWNLPLALAATPIVTAVFPWFSSMAVDGTLLATFEDRVRKTVGL
                     MIYIMVPCIAGLVVLAQPIVRLLFERGAFDASATSLTAACVQMYCLGLIGQALFPVFH
                     RVFYSFKDTRTPLVVGVVMIAINAVTDWLFGMWFGAAGIALSTTAAVTVGAIMDALLI
                     RRFFRGEVRTTIRYPLLRESLKTLAAVIPVVGVALLCRAWIGAAQGFAHLALRLVLVC
                     LAAAVCYMISSVALHIDGWRILLGRIRRRVTRGNEALSTDTQDL"
     gene            complement(2050..>2611)
                     /locus_tag="VN478_03540"
     CDS             complement(2050..>2611)
                     /locus_tag="VN478_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21779.1"
                     /translation="WNLLGSTCAGMLAGGVTGPVTDAARSAFVQGQRLVPDDYRPWWS
                     QGMMEINLKSPAALTCLQEAERLYPTLAAIKGWLALAMIYVGGDVVGAEAKAAEALAI
                     TKGDPYAVTAQAFCALARGETSRAKELLTSIVTQGFTNKFAYYGLSLCYRAVGDTAME
                     RTELQFSGRINPNLVEAVTRLRDLSR"
BASE COUNT          394 a          823 c          855 g          539 t
ORIGIN      
        1 cagtgatttg gactacgacg aacgtgtgcg tttggacatg gattacatca cgcactggag
       61 tctgtggctg gacctgcgca tcctggcgtc gaccatcccg gcggtcctgc gcaaaaaggg
      121 agcgcgctag ctttggggtc agctggcgat caggtaccga gtggtacatt taggaactcg
      181 ggagccttgc acagaaaggc tcagggggga atgacagggt tgctgtcggg gaggctggtg
      241 catgtaccac cttccattat ttgccagctg accccaaagg gtccacggtt tggtggttgc
      301 gctcgtcgtt cctggtgcta tcgtgctaga gtctcgtttg gggaggatca ccggtgacag
      361 aggatagcgg aggacgatcg accagggata gcgcgatgtc gcacggccgc gggctgctgc
      421 gcaggcagtc ggttgcgcag gcggcggtgt ttctgagcgt cgttacgctg ttcagcaaat
      481 tcatcggctt cgcgcgcgac gcgatcgtgg cccacaactg gggtgcgacc ggcctgaccg
      541 acgccttcct catcggcatg atggtgccca gtgtgctgtt gggcatcgtg tcgacgggac
      601 tcagcacgat catcgtgcca tggtacatcg cccaccgcaa ggacgacccg gcgcgtgcgc
      661 gggaactggt caaccaggtg acgtttgtct gggggatggc cttcctcgcg gcgtcattgc
      721 tcgtcgtcgc gttcgcccca cagctggttc acctgttcgc gccaagcttc acggacttcc
      781 ggtacgacgt ggccgtcaag gtaacgcgtc tgctggtccc gatgggtttc ttcaacgtga
      841 tgaccgggct ggtgacgggg ctgtcgcagg cggaaggcca gttcctgatc cccctgttgt
      901 ccaacgtgtt cgggaacgtc ctgatggttg ggtgcctgat cttcttcgcg accccactgg
      961 gaatccagag ctactctctg ggccagactg tgatgtcggt catcggcttc gcgccggtcc
     1021 tctggttcct tgtgcgccgc agaggcttct ttcggcacct tgacatgcgg catgtggact
     1081 ggcgggcgat cgggtccttt gccgccctcc tggtgccgct ggtcatctcc ggaggtggcg
     1141 ggtccatgaa cacagtcgtg gaccgctggt tcgcctcgca cctttcgcag gggagcggtt
     1201 cggcgctcga ctactcaaac cggctctgga atctgccgct ggcattggcg gccacgccga
     1261 tcgtcacggc cgtcttccca tggttctcat cgatggccgt ggacgggacg ctgcttgcga
     1321 ccttcgagga ccgcgttcgg aagacagtgg ggctcatgat ctacatcatg gtcccctgca
     1381 tcgcggggct ggtcgtcctt gcacagccca tcgtgcgcct cctgttcgag cgaggcgcct
     1441 tcgacgcgtc agccacgagc ctcaccgcgg cgtgcgtcca gatgtactgc ctgggcctca
     1501 tcggccaggc actgttcccc gtgtttcacc gtgtcttcta ctcgttcaag gatacgcgga
     1561 ccccgctggt cgtcggggtg gtgatgatcg cgatcaacgc cgtcaccgac tggctctttg
     1621 ggatgtggtt cggcgcggcg ggcatcgccc tctcgacgac cgccgcagtg accgtcggcg
     1681 cgatcatgga cgctctcctg atccgcaggt tcttccgggg cgaagtgcgg acgaccatcc
     1741 gctatccgtt gctgcgcgag tctctgaaga cactggcggc cgtgatcccg gtcgtgggtg
     1801 tggcgctcct gtgtcgggcc tggatcggtg cggcccaggg atttgcccac cttgccctgc
     1861 ggctggtcct ggtctgcttg gccgccgcgg tgtgctacat gatctccagc gtggctctgc
     1921 acatcgacgg ctggcgcatc ctcctcggcc gcatcaggcg ccgcgtgacg cgtgggaacg
     1981 aagcactgtc caccgacaca caggacctct gacgaaacgc gaggtcggac gtgccctgac
     2041 cgagccttcc tatcgggaca ggtccctcaa gcgagtgaca gcctccacga ggttggggtt
     2101 gatgcggccg gagaactgca gctcggtgcg ctccatcgcg gtgtcaccca cggcacgata
     2161 gcagagggac aagccatagt aggcgaactt gttggtgaac ccctgggtga cgatgctggt
     2221 cagcaattcc tttgcgcgtg aggtctcgcc ccgtgcgagt gcgcagaagg cctgcgccgt
     2281 gacagcgtac ggatctccct tcgtgatcgc cagcgcctcg gcagccttgg cctccgcccc
     2341 gacgacgtcg ccgcccacgt agatcatggc cagcgccagc caccccttga tggcggccag
     2401 cgtcggatac agccgctcgg cctcctgcag gcaagtcagc gctgcagggc tcttgaggtt
     2461 gatctccatc atgccctggc tccaccatgg ccggtagtcg tcgggcacca gcctctgtcc
     2521 ctggacaaag gcagaacgcg ccgcgtcggt gaccggaccc gtcacgcccc cggccagcat
     2581 gccggcgcag gtgctgccca gcaggttcca g
//
LOCUS       DATMZD010000172         3563 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_85494,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000172 DATMZD010000000
VERSION     DATMZD010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85494"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="VN478_03545"
     CDS             complement(<1..733)
                     /locus_tag="VN478_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21780.1"
                     /translation="MASIRKAVLVMVSMCIILGSPFVGTLPRAAADIVFDSVTATPAT
                     SGSAAAFSVSMHVQQDVPANANHFIYARFPVSFTIPSSIPTTAVMLKGVYAPSVVTVE
                     RGVDGTTVGFDVVGFVGYPSLLKWEPVTISILDTAGIHLPSLSGIYPVYLWTDVEPNP
                     VRCNLAVQQSGGNGQSVKGLAVVLGTTFAAGKAAQYDLTFETTPAGSLMAAKGDFIDI
                     VFPDGTIIPSNLDPASILLRYQNCTK"
     gene            complement(943..>3563)
                     /locus_tag="VN478_03550"
     CDS             complement(943..>3563)
                     /locus_tag="VN478_03550"
                     /inference="COORDINATES: protein motif:HMM:NF020700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HWQ21781.1"
                     /translation="LKVGYWTPVPVTDYEEVDETVTFTGPLSYMSGTRGFDGDGYPYY
                     MSGEWYHFRITKAGAIGASIDTTMWQRQDPAYPLDANNACCKTFTKTFTVCEIKSCTT
                     TSVQLTGGAQTSASAITVGGKSDLVVSVDPTGAPADLFCGCNSKIVHIYMTNSCGVTQ
                     DAFTVDTYGGGTPVTVDELWYNGIPESLASYGATATCPAPTGYSADGTVLFQQTTPGI
                     VAVDNCTTLTFKGVTINFPTGTDCGYRLVVQVFGEQRGYDQCGVLCPTFPFISESINP
                     ISINPVTVTKTATATIYEAGIDPTQILAGVPATVEITDPAFSSKTSSKWEDATANWSF
                     SFKAAGDDTFHAFEYWGLTAPTVSSIDNGYRFSSFCINRAGTIEIYTYSYNTDNDCKQ
                     LEELVVDVEVVMPVFGVKIELLDGTMLENDNILTSGFGETVYVTATDPRTDGTHDFTT
                     DSSWHLTAAGRTDSCYLRTALVCLSSPTGCIGCAGGNALTITAYDNPCLEGAGTVRLS
                     MVTACGNLKISDFTVTDPTVTVDPATVAFTIPASATHVTFTVKDAHGHGAPGITVSIS
                     GDMSPSTGASGYSWTAGSVETGKDPMGEADWAFVPPFSGQYFVSAIGSEYCTIRANDS
                     ETLVQNPPLVWTTKYAKLGATITAKYQAPVVDTTAPVVTVTAGIDGSTVGAEVLALTG
                     KVTDNVGVSQLYVGFNKVDVLPDGSFMTNVKLAAGENTITVVAYDAAGNKGTAAVKVT
                     YTAAVDTKTVLILTIGADIVSVNGKATSIDAAPEIVASRTFVPMRFIAESFGATVEWL
                     PETQGITITLGDHTIGLQIGNATAVIDGTIVSLPAAPYIKNGRTMVPLRVISEAFGGD
                     VVWDPAARTITITYIP"
BASE COUNT          783 a          964 c         1123 g          693 t
ORIGIN      
        1 ccttggtaca gttctgatac cgcagaagaa ttgaagccgg atccaggttc gagggaatga
       61 tcgtcccgtc cgggaacacg atatcgatga agtcaccctt cgcggccatc aggctgcccg
      121 ccggagtcgt ctcgaacgtg agatcatact gcgccgcttt gcctgcggca aacgtcgttc
      181 caagaaccac tgcaagcccc ttcacggatt gcccgttccc gccggattgc tgtacggcaa
      241 ggttgcaccg aacagggttc ggctcgacat ctgtccacag gtacacaggg tagatgccag
      301 acagcgaagg gagatgaatg cctgccgtat cgaggatcga tatcgtgaca ggttcccact
      361 tgaggagact cgggtacccg acgaatccga cgacgtcgaa acccaccgtc gtaccgtcca
      421 cgccgcgctc gacagtcacg accgaaggag catagacccc cttgagcatg acggcagtcg
      481 tcggaatgct ggatggaatg gtgaatgaga caggaaatcg ggcatatatg aagtggttcg
      541 cgttggcggg tacgtcctgc tgcacgtgca tgctgacgga aaatgcagca gcactgccgg
      601 aggtggcggg cgtcgcggtg acgctgtcaa atacgatgtc cgctgcagct ctggggagtg
      661 tgccgacaaa aggcgaacca aggataatgc acatggaaac catgacaagt actgctttcc
      721 ggattgatgc caatactctg tacctccaca ctgtgtcaac aaactcgtcg acactgtatg
      781 aaccgattat acgtcgaaca gaccctgatg caacgacatc tggagcgcct ctgtgtcaca
      841 tttgtgactc accctggctg ccccgcggga gccgggaaat ggaaaagggc cccgctctca
      901 cgagcggggc cctttctagt cctgtactat gccgtaccta ccttacggaa tgtaggtgat
      961 ggtgatggta cgagcggctg gatcccagac gacgtcgcca ccaaaggcct cggagatgac
     1021 tctcagagga accatggtgc gaccattctt gatgtacggg gcagcgggca gggagacgat
     1081 ggtgccgtcg atgactgccg tggcattgcc gatctggaga ccgatcgtgt ggtcgcccag
     1141 agtaatcgtg atgccctgag tctcaggaag ccactcaacc gtggcgccaa aggactcagc
     1201 gatgaaacgc atcggaacaa acgtgcggct ggcaacgatt tctggggcag cgtcgatgga
     1261 cgtggccttg ccgttgacag acacgatgtc agcgccgatg gtcaggatga ggactgtctt
     1321 ggtgtcgacg gcggccgtat aggtcacctt gaccgcagca gtacccttgt tgccagcagc
     1381 atcataggca accacagtga tggtgttctc gcctgcagca agcttcacgt tggtcatgaa
     1441 ggagccgtcg ggaaggacat caaccttgtt gaagccaacg tacagctggg agacgccaac
     1501 gttgtccgtg accttaccgg tgagggcaag gacctcagca ccgacggtag aaccgtcaat
     1561 gccggcggta acggtgacga caggagcagt ggtgtcgaca acaggagcct gatacttggc
     1621 cgtgatggtg gcgccaagct ttgcgtactt ggttgtccag acaagcggag gattctggac
     1681 caaggtctca ctgtcgttcg ctctgatagt gcaatactcg ctcccaatcg ctgaaacaaa
     1741 gtactggcct gagaaaggcg gaacgaatgc ccaatcggct tcgcccatcg ggtccttgcc
     1801 ggtctcaacg ctgccagctg tccaggagta cccggacgcg ccagtggacg ggctcatgtc
     1861 gccagagatg ctgaccgtga tgccaggggc accgtggcca tgtgcatcct tgacggtgaa
     1921 ggtcacgtgg gtcgcggacg cagggatcgt gaacgctacg gtcgcgggat caaccgtgac
     1981 agtcgggtca gtgaccgtga aatccgagat cttcaagtta ccacaagcgg tgaccatgga
     2041 aaggcgaaca gtgccggcgc cttccaggca ggggttgtca taagcggtaa tcgtgagtgc
     2101 attgccgccg gcgcaaccga tgcagccggt aggagaactc aggcagacga gcgcggtacg
     2161 cagatagcag ctgtccgtac ggccggcagc ggtgaggtgc cacgagctgt cagtcgtgaa
     2221 gtcatgcgtg ccatcagtac gcgggtcagt agctgtaacg tagactgttt cgccaaatcc
     2281 ggaagtcagg atgttgtcgt tctcgagcat ggtaccatcg agcagctcga tcttgacgcc
     2341 gaacacgggc atgactacct caacatcgac gacgagctct tcgagctgct tgcagtcatt
     2401 gtcggtgttg tagctgtacg tgtagatctc gattgtgcca gcacggttga tgcagaacga
     2461 agagaaccga tagccgttgt cgatcgagga aacggtcgga gcagtcagtc cccagtactc
     2521 gaacgcatgg aacgtgtcgt cacctgcagc cttgaaggag aaggaccagt tggcggtcgc
     2581 atcttcccac ttgctgctgg tcttgctgga gaatgctgga tcggtgatct cgacggtcgc
     2641 gggaacgcca gccaggatct gggtcgggtc aataccggcc tcgtagatgg tggcagtcgc
     2701 cgtcttggtg acggtaacgg ggttgatgct gatcgggttg atcgactcgc tgatgaaggg
     2761 gaaggtgggg cacagcacgc cgcactgatc atagccgcgc tgttcgccaa agacctggac
     2821 cacgagacga tagccacagt cagtacccgt cgggaagttg atcgtaacgc ccttgaaggt
     2881 cagcgtcgtg cagttgtcca ctgccacgat gccaggggtc gtctgctgga agaggacggt
     2941 accgtcggcg ctgtacccgg taggagcagg gcacgtcgcc gtagcgccat aggacgccag
     3001 cgattctggg atgccgttgt accacagctc atccacggta acgggagtgc cgccgccgta
     3061 ggtgtcgaca gtgaaagcat cctgggtaac gccgcaggag ttggtcatgt agatgtggac
     3121 gatcttgctg ttgcagccgc agaacagatc ggcaggagca ccggtcggat caacgctgac
     3181 aacaaggtcg gacttgccgc ccaccgtaat agcagaggcg ctggtctgtg caccgccggt
     3241 cagctgaacg ctggtggtcg tgcaggactt gatctcgcag acggtgaacg tcttggtgaa
     3301 cgtcttgcag caggcgttgt tcgcatccag cggataggct gggtcctgac gctgccacat
     3361 ggtggtgtcg atagaggcgc caatggcacc cgccttggtg atgcggaaat gataccactc
     3421 gccggacata tagtacggat agccatcgcc gtcgaacccg cgggtacctg acatgtagga
     3481 cagggggccc gtgaaggtaa cggtctcgtc gacttcttca tagtcggtca caggaacagg
     3541 agtccagtag ccaaccttga gcc
//
LOCUS       DATMZD010000173         2172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_65046,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000173 DATMZD010000000
VERSION     DATMZD010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65046"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..1350)
                     /locus_tag="VN478_03555"
     CDS             complement(46..1350)
                     /locus_tag="VN478_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018963492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21782.1"
                     /translation="MIYAIASLCDWFGAKRAAMRLFAVAPHSDYAAWWGAVGLMQTGR
                     DEEALALLTRVRAIHPEWKRTKRLLATLYLRRDPERAVQLYTPPVGIWDEVCLGDLLY
                     FFLHREDEGVEWWRRAYARIDWESARELDNPARLLLKRLCRITHDPALLERFAELDTD
                     NFRQCDIVDYADILAKRGEVATAREMLDRGFYIYRGDPVLTACWERLAFGQLPPYKVK
                     TSGTAAVRHNVYTGLLTEATDLSSIVDRVHEEHPTGVVTIASSVMTMCEGTLMWIGTF
                     KPSRLARFLGPYTGHGNGAFVHWYSYPKEAAWKVQAYIELAGAFRVMLGAAATVLGKL
                     LHRKGWFYEVVGPVAKAVDSDKVMPYDACLVPGPLDVEASIAALARKGARISVVDVND
                     VFGAEIVASTEGVDGDWLERSLEDNPAGNDDSMTPIVVVMPE"
     gene            complement(1373..>2172)
                     /locus_tag="VN478_03560"
     CDS             complement(1373..>2172)
                     /locus_tag="VN478_03560"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HWQ21783.1"
                     /translation="AEAHGVTVVPLKVSLDGEYFRDGIDIANDEFYRRLVAGAKAGST
                     QPSPDEFASAYRAILDKDPDGDIVSVHISSRMSGTLNSALTGRNQLDTVHVRFVDSWN
                     AALGVGFPVMRAVELAEAGAPLWQVVAETEALCLRTHTYFVLDSLKYLERGGRIGKAA
                     AIAASILKIKPVLTYIEGVTDVVDRPRTKKVAFEHLWAIVDKHVQKGTEYIGFHYGAN
                     QGEVEAFQREFTAKYNIPSILVQLGPVVGTYSGPDMIGVTITEKKTS"
BASE COUNT          392 a          715 c          639 g          426 t
ORIGIN      
        1 agcatcacct gctcccgcct cttcttttgc acttgttcgc agtcgctact caggcatcac
       61 gacgacgata ggcgtcatcg agtcatcgtt gcctgccggg ttgtcctcca gcgagcgctc
      121 gagccagtcg ccgtccacac cctcggtcga ggccacgatc tcggccccga agacgtcgtt
      181 gacgtcgacc acgctgatgc gggccccttt gcgcgccagc gcggcaatac tcgcttctac
      241 gtcgagcgga ccaggcacga ggcatgcatc gtatggcatg accttgtcgc tgtccacagc
      301 cttggccacg ggaccaacga cctcgtagaa ccagcccttg cggtgcagca gcttccccag
      361 cactgtggcc gctgccccca gcatgacgcg gaaggccccg gccagttcga tatacgcctg
      421 caccttccac gcggcctcct tcgggtagct gtaccaatgc acgaaggcgc cgtttccatg
      481 ccccgtgtat ggcccaagga accgcgccag gcgtgacggc ttgaacgtac cgatccacat
      541 cagggtcccc tcgcacatgg tcatgacgct gctggcaatc gtgacgactc ccgtagggtg
      601 ctcctcgtgg actctgtcca cgatcgacga gaggtcggtc gcctcggtaa gcagaccggt
      661 gtagacgttg tgtcgtacag cagcggtccc tgaggtcttc accttgtacg gcgggagctg
      721 gccgaaggca agtcgctccc agcatgccgt caacaccggg tccccgcgat agatgtagaa
      781 gccgcggtcc agcatctccc tcgcggtagc cacttcgccg cgcttcgcca ggatgtcggc
      841 gtagtcgacg atatcgcact gacggaagtt gtcggtgtcc agttctgcaa accgctcgag
      901 caacgccggg tcatgcgtga tgcggcacag cctcttgagc aggaggcgcg cggggttgtc
      961 cagttcgcgg gcactctccc agtcgatccg tgcatacgcc cgtcgccacc actccacgcc
     1021 ttcgtcctcg cggtgaagga agaagtacag caggtccccg aggcagacct cgtcccagat
     1081 gcccaccggc ggagtgtaca gctgaacggc cctctcggga tcgcggcgaa gatacagcgt
     1141 cgcgagcaga cgcttcgtgc gcttccactc cgggtggata gctctcaccc gtgtcaggag
     1201 agccagcgcc tcctcgtccc tgccggtctg catgagaccg accgcccccc accatgcggc
     1261 gtagtctgaa tgtggagcaa ctgcaaacaa tcgcatggcg gcgcgcttgg cgccgaacca
     1321 gtcgcacaac gaggcgatgg cgtagatcat gttttctcct gtcacttgtg gattatgacg
     1381 tcttcttttc cgtaatggtg accccgatca tgtcaggccc cgaatacgtc ccgacgacag
     1441 gcccaagctg aaccagaatg cttggaatgt tgtacttggc ggtgaattcg cgctggaacg
     1501 cctcaacctc gccctgattg gcgccgtagt ggaagccgat gtactccgtc cctttctgca
     1561 cgtgcttgtc gacaatcgcc cacaagtgtt cgaacgcgac tttcttagtg cggggacggt
     1621 ccacaacgtc cgtgacccct tcgatgtacg tgaggaccgg cttgatcttc aggatgctgg
     1681 cggcgatggc agcagccttt ccgatgcggc cgcctctctc aagatacttg agggagtcaa
     1741 gcacgaagta cgtgtgcgtg cgcagacaca gcgcctcggt ctcggcaacc acctgccaca
     1801 gaggcgcccc cgcttcagca agttctactg cccgcatgac aggaaagccg acgcccagcg
     1861 ccgcgttcca gctgtccaca aaccggacat ggactgtgtc cagctggttg cgcccggtga
     1921 gcgccgagtt gagtgtgcct gacatgcgtg agctgatgtg cacagagacg atgtccccat
     1981 cggggtcctt gtccaggatg gcgcgatacg ccgacgcgaa ctcgtccggt gatggctggg
     2041 tgctgcccgc cttggcgccc gctaccaggc gtcggtagaa ctcgtcattt gcgatgtcga
     2101 tgccgtcacg gaagtattct ccgtccagag agaccttgag cggcacgact gtgacgccat
     2161 gcgcttctgc at
//
LOCUS       DATMZD010000174         2013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_65065,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000174 DATMZD010000000
VERSION     DATMZD010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65065"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1017
                     /locus_tag="VN478_03565"
     CDS             <1..1017
                     /locus_tag="VN478_03565"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HWQ21784.1"
                     /translation="GCGERGNEMTDVLQEFPQLKDPKTGEVLMNRTVLIANTSNMPVA
                     AREASVFTGITIAEYFRDMGYSVALMADSTSRWAEAKREISGRLEEMPGEEGYPAYLA
                     SRVAAFYERAGRSLSLGSQPREGSLSVIGAVSPPGGDLSEPVVQATLRAVKVFWALDS
                     RLAYARHFPAIDWLQSYSLYSDLLEHFYDKELGEGWSQDIREVRALLQKEAELQELVR
                     LVGVDALSAADRLVLESAKSIREDFLQQGAFTEGDSYTSLQKQKRMLGAIMLWSHLAG
                     KASMNGVSLDAILEMPVRVEIARSKNIPESNLTSFDALEHAIEKGFGTLSETDTTARE
                     EYHE"
     gene            1010..>2013
                     /locus_tag="VN478_03570"
     CDS             1010..>2013
                     /locus_tag="VN478_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012269820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HWQ21785.1"
                     /translation="MNKDYRTVREIVGPLVLLDGAQGVKYGELVELRTVSGLRHGQVL
                     EVSGDTVLVQVFEGSSGMDRGQTVTRFLGHGLQFGVSRDIVGRVFNGLGAPIDGIAPI
                     LPDKVVDINGDPMNPYARDYPDEFIQTGISAIDGLNTLVRGQKLPIFSGSGLPHAQIA
                     AQIARQARVLSGSDEFAVVFGAMGITFEEADYFISDFRKSGAINQSVLFMNLASDPVI
                     ERIATPRFALSAAEYLAFELGMHVLVILSDMTNYCEALREISAARKEVPGRRGYPGYL
                     YTDLATIYERAGRIKDKRGSITQLPILTMPEDDKTHPIPDLTGYITEGQIFLSRSLHQ
                     A"
BASE COUNT          413 a          565 c          612 g          423 t
ORIGIN      
        1 ggctgtggag agcgcgggaa cgagatgacg gacgtcttgc aggaattccc acagcttaag
       61 gaccccaaga ccggcgaagt cctgatgaat cgcacggtcc tcatcgcaaa tacgtccaac
      121 atgccggtcg cagcgcgcga ggcatccgtg ttcaccggaa taacgatagc cgagtacttc
      181 cgcgatatgg gctacagcgt cgccctgatg gcggactcga cgtcccggtg ggcggaagcc
      241 aagcgtgaga tctctgggcg actcgaggaa atgcccggtg aagagggata tccggcgtac
      301 ctcgcgtcac gtgtggcagc gttttatgaa cgagctggga ggtccctgag tcttggcagt
      361 cagccgagag aaggcagctt gagcgtcatc ggtgctgtct ctcctcctgg cggcgacctg
      421 tccgaaccag tcgttcaggc cactctcagg gcagtcaagg tcttctgggc actggattcc
      481 cgccttgcct acgcgaggca cttcccggcc atcgactggc ttcagtccta ctcgctgtac
      541 agtgacctcc tcgagcattt ctacgacaag gagcttggtg agggctggag ccaggacatt
      601 cgcgaggtgc gggccctgct tcagaaggaa gctgagctgc aggagctggt ccgccttgtt
      661 ggtgtggacg cgctgtctgc agcggatcgg ctggtactgg agtctgcgaa gtcgattcgt
      721 gaggacttcc tccagcaggg agccttcacc gagggcgaca gctacacttc tctgcagaag
      781 cagaagcgaa tgcttggtgc catcatgctc tggagtcatc ttgcaggcaa ggcttccatg
      841 aatggggtat cgctggacgc cattctcgag atgccggtca gagtcgagat cgcccgctcg
      901 aagaacattc ccgagtcgaa ccttacgtca ttcgacgcgc ttgagcacgc gatagaaaag
      961 ggctttggga ctctgtcgga gaccgacacg actgcacggg aggagtacca tgaataagga
     1021 ttaccgcaca gtccgcgaaa tcgtagggcc gcttgtcctt ctggacggag cgcagggcgt
     1081 caaatacggt gaactggtcg aactgcgtac tgtctcagga ctgaggcacg gccaggtgct
     1141 cgaggtctcc ggggacactg tcctggttca ggtcttcgag ggttcgtcgg gcatggatcg
     1201 cggccagacc gttacccggt tccttggaca tggactgcag tttggagtct cgcgcgacat
     1261 agtaggcaga gtgttcaatg ggctgggagc tccaatcgat ggaattgccc ccattctgcc
     1321 agataaggtc gtggacatca acggtgatcc catgaaccct tacgcgcgtg attatccgga
     1381 tgagttcatc cagactggca tctcggcgat tgatggcttg aatacgcttg tccgtggcca
     1441 gaagctgccg atcttctcag gcagtggtct cccacatgct cagatcgctg cgcagattgc
     1501 caggcaggct cgggtgcttt cggggtcgga cgaatttgcc gtcgtctttg gcgccatggg
     1561 tatcaccttc gaggaagcag actacttcat ttcggacttt cgtaagagtg gagccatcaa
     1621 tcagtctgtg ctgttcatga acctggccag cgatccggtc atcgaacgca tagcaacgcc
     1681 tcgtttcgcg ctgtctgcag ccgagtactt ggcatttgaa cttggaatgc acgtcctggt
     1741 catcctctcc gatatgacca actactgcga agccttgcgg gagatctccg cggcacgtaa
     1801 ggaagtcccc ggacgacggg gataccccgg atatctgtac acggatctgg cgacgatcta
     1861 cgagcgagcc ggccgcatca aggacaagag ggggtcaatc actcagctgc cgatcctgac
     1921 catgcccgaa gacgacaaga cccatcccat tccggatctt accggataca tcactgaggg
     1981 ccagatcttc ctgagccggt ccctacatca ggc
//
LOCUS       DATMZD010000175         2088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_105533,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000175 DATMZD010000000
VERSION     DATMZD010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105533"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1551
                     /gene="dnaK"
                     /locus_tag="VN478_03575"
     CDS             <1..1551
                     /gene="dnaK"
                     /locus_tag="VN478_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HWQ21786.1"
                     /translation="IKNDAEAFLGQKVSKAVITVPAYFNDAQRQATKDAGEIAGLEVV
                     RLINEPTAAAFAYGLDKTEGEHKILVFDLGGGTLDVTIMDFGGNVFTVASTSGDTQLG
                     GTDMDNALLDYVAEDFRSQSGIDLRVDKMAMQRLREGVEKAKVELSTTFETTINLPFI
                     TADASGPKHLVMPITRAKLESLVQPIIDRCREPVNRALADAGLTPQQIDNIILVGGPT
                     RMPIVQKFVESYFSKTPERGVDPMECVAMGAAIQGGIMGGEVKSGMVLVDVTPLTLGI
                     ETLGGVFTEMIKRNTAVPISKTQTFTTASDSQTQVEIHVLQGERPMAKDNMSLGRFIL
                     DGIAPARRGEPQIEVSYDIDANGIVHVTAKDKATGKEQHITIENATKLKDDEIERMKR
                     EAEQFADQDKRVKEEVELKNRADQVVFSGRKTLTENTARISPDVKAEAERKLTELEDL
                     VRDNKTSEIEAKIDEVNTAFSKVTEDLQHQAGQQQEPTASQQQNESGTQQPPQDGQTV
                     EGQGTAKQ"
     gene            1597..>2088
                     /locus_tag="VN478_03580"
     CDS             1597..>2088
                     /locus_tag="VN478_03580"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HWQ21787.1"
                     /translation="MATDRDYYDVMGVPRGASQDDIKKAYRELVKKYHPDLHKDDPQA
                     PRNMSDVNEAYDTLSDPAKRQQYDTYGKAGPAGFPGAGQAWAPQGSGSGETFGGFDIG
                     DLFGSFMGGFGHQQAEETPRGRDLSFGMTLTLEEAVFGVKKEIQVRRYDTCATCHGTG
                     AAPG"
BASE COUNT          512 a          564 c          648 g          364 t
ORIGIN      
        1 atcaagaatg atgcagaggc gtttctcgga cagaaggtgt ccaaagcggt gattacagtc
       61 cctgcctact tcaatgacgc tcagaggcag gcgaccaagg atgccggcga aatcgccggg
      121 cttgaggtcg tgcgcctgat aaatgagccc accgccgcag catttgccta tggcctggac
      181 aagacggaag gcgagcacaa gatcctcgtg ttcgaccttg gtggtggcac gctggacgtg
      241 acgataatgg acttcggagg gaatgtgttc accgtggcct cgacgtcagg cgatacgcag
      301 ctcggcggaa ccgacatgga caatgctctt ctggactatg ttgcggaaga cttccgtagt
      361 cagagcggca ttgacctgcg cgtggacaag atggcgatgc agcgccttcg tgaaggcgtg
      421 gagaaagcca aggttgagct ttcgacaacg ttcgagacga ccatcaacct tcccttcatc
      481 acggcggacg cgagcgggcc gaagcacttg gtgatgccca ttacacgggc caagctcgag
      541 tcacttgtac agcctatcat cgacagatgt cgcgaaccgg tgaaccgtgc tctcgcagac
      601 gcgggtctca caccgcagca aattgacaac atcatccttg tgggtggtcc gacccgtatg
      661 ccgatcgtcc agaagttcgt cgagagctac ttcagcaaga ctcctgaacg tggcgtggac
      721 cctatggagt gcgtcgccat gggtgctgcc atccagggtg gaatcatggg cggcgaggtg
      781 aagagtggga tggtcctggt tgacgtgacc cccctcactc tcggcatcga gactctgggc
      841 ggcgtcttca cggagatgat caagcgcaac acggccgtgc ctatctccaa gacccagacg
      901 ttcaccacag cgagcgacag tcagacgcag gttgagatcc acgttcttca gggcgagcgc
      961 ccgatggcga aagacaacat gtctctagga cgtttcattc tggatggcat cgctcccgcg
     1021 cgtcgcggtg agccgcaaat cgaggtcagc tatgacatcg acgcaaacgg cattgtccac
     1081 gtcacggcca aggacaaggc tacgggcaag gaacaacaca ttacgatcga gaacgcgacg
     1141 aagctgaaag acgacgaaat cgagcggatg aagcgcgagg ccgaacagtt cgccgaccag
     1201 gacaagagag tcaaggaaga ggtcgagctc aagaaccgtg cggatcaggt tgtattcagc
     1261 gggcgcaaaa cgctgacaga gaacacggcc aggatctctc ccgacgtcaa ggcggaggca
     1321 gagaggaaac tgactgagct cgaggacctc gtacgcgaca acaagacttc cgaaattgaa
     1381 gcgaagatcg acgaggtcaa tactgctttc tccaaggtca cagaggacct gcagcatcag
     1441 gctggccagc agcaggagcc gacagcttct cagcagcaga atgagtcggg gactcagcag
     1501 cctccgcagg acgggcagac cgtcgagggt caggggactg caaagcagta ggtacggcaa
     1561 tacaaggcaa gcgctaatat ctgagtggag gtcgatgtgg cgactgatcg cgattactac
     1621 gacgttatgg gagtgcccag gggggcctcc caggatgata tcaagaaagc ctatcgtgaa
     1681 ctggtcaaga agtatcatcc cgacctccac aaggatgatc cccaagcacc cagaaacatg
     1741 tcagacgtga atgaggcata cgacacgctc agtgatcctg cgaagcgtca acaatacgat
     1801 acctatggca aggccggccc ggcaggtttt cctggcgcgg gccaggcatg ggctccccaa
     1861 ggcagcggct ccggcgagac atttggcggt ttcgacatcg gtgacctctt cggcagtttc
     1921 atgggggggt tcggccacca gcaggccgag gagacgccac gcgggcgaga cctcagtttt
     1981 ggcatgacac ttacgcttga agaagccgtg tttggtgtca agaaggagat ccaggtaagg
     2041 cggtatgaca cgtgtgcgac gtgccacggc acaggagctg ctccaggt
//
LOCUS       DATMZD010000176         2053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_35867,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000176 DATMZD010000000
VERSION     DATMZD010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35867"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VN478_03585"
     CDS             complement(<1..273)
                     /locus_tag="VN478_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21788.1"
                     /translation="MTEQGAHRHVVLRGRFDTARILPGLPAVHAIPVRDARMRGSVAG
                     AVPIGTAVIDLVPAVLFSFASNPVTYRRVDAVYQRDPQRFGLLASRS"
     gene            complement(270..1058)
                     /locus_tag="VN478_03590"
     CDS             complement(270..1058)
                     /locus_tag="VN478_03590"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HWQ21789.1"
                     /translation="MRRAAGWILPVIAVAATVIVSVGFGHNHVVEPRAIAYVQSGDVW
                     RARQHLLGQEADVVYGAQVAQQERSLQAAEAEEAADVVAVHAGDHDAMIRQSNREDQL
                     IRSVIAGVDPAVAMQLPEVEELAWPLTGPMKITSPYGERIHPIIGEEAFHRGVDLRAH
                     YGSPILAPADGVVVYVGAKTAYGNVIVIVHGGGIATVYGHLWKFAVQPCERVKEGQLL
                     GYTGNTGFSTGPHLHFEVRQNGEPTNPLEWLPPIETTDQGAIIR"
     gene            complement(1351..>2053)
                     /locus_tag="VN478_03595"
     CDS             complement(1351..>2053)
                     /locus_tag="VN478_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21790.1"
                     /translation="MQVAQMSQPDSPGPGDVWQAAFLLAGTDVGLDDGTAVGILAGLF
                     ALRRAPAKQSLPVPASSLAVDAEDDDEDLRKVRGDLVTQLREVRRLTGELDKVRERLS
                     ARDAENSRLMGSLAARAEPEAEVQYTVHPITERILVAGGHETLSRNLQTWLPNSVCIA
                     TNGKEDLDPSVLATTRLVVVLTSYVSHAFSGKVISEAHKRDLPVLPLDWRSAKHILQE
                     IDHALTTQPDLSAKQ"
BASE COUNT          381 a          666 c          635 g          371 t
ORIGIN      
        1 gctcctggac gccagaagac cgaatcgctg cggatcacgc tgatagacgg cgtcaacccg
       61 tcgatacgtc acgggattgg atgcgaaact gaacaggact gccggcacaa ggtcgatgac
      121 ggcagtgcca atcgggaccg ccccggccac cgacccgcgc atgcgcgcgt cccgcactgg
      181 aatggcatga actgcaggga gacccggcag aatgcgtgcc gtgtcgaacc gccctctcag
      241 gacgacgtgc cggtgggcgc cctgctcggt caccggatga tcgccccctg atcggttgtc
      301 tcgatgggag gcagccactc cagcgggttg gtcggctcac cattctgacg cacttcaaag
      361 tgcaggtgag gtcctgtgct gaagcccgtg ttgcctgtgt acccgaggag ttgcccttcc
      421 ttgacacgct cgcagggctg gacggcaaac ttccaaaggt ggccgtagac ggtggcaatg
      481 cccccgccat gtacgatgac aatcacgttg ccgtacgcgg tcttggcacc gacatacacg
      541 acgacgccgt cagcgggggc aaggatgggg gagccatagt gagcacgcaa gtcgacgccc
      601 ctgtggaacg cctcttcacc gatgatgggg tgtatgcgtt cgccgtaggg agatgtgatc
      661 ttcatcggtc ccgtgagcgg ccaggcgagt tcctcgactt ctggaagctg catggcgaca
      721 gcggggtcga cgccggcgat gacagaccgg atcagctggt cctcccggtt gctctggcgt
      781 atcatggcgt cgtggtctcc ggcatggacg gcgactacgt ctgcagcctc ctcggcctcg
      841 gctgcctgca ggctccgctc ttgctgtgcg acctgagcgc cgtacacaac gtcggcctcc
      901 tgcccgagga gatgttgtct ggcccgccac acgtcgccgg actggacgta cgcgatggca
      961 cgcggttcga cgacatgatt gtgcccgaac ccgaccgaca cgatgaccgt cgctgcaact
     1021 gctatcacag gcaggatcca tcctgccgcc ctgcgcattc tcactctgca gacctccacg
     1081 ttcatgacac gggctcaatt ggacccgacc tttcattata ggacagtcgg cggccccaac
     1141 aacggcggcc agctgaaaca ctgattctca cttgcgcagg ggctgtgcca tggtgagaga
     1201 tggattcccg catgcgctgc gactcacgcg accacgcaac gatggagtcc cgccttcgcc
     1261 gaaagcaccc ctcggattga acagaagtcc tcgagggaaa gcactcctcg tgctggactg
     1321 acacactctt ggggggaacg acggcgtatc ctactgcttc gctgaaaggt ccggctgcgt
     1381 cgtcaacgcg tggtcgatct cctgcaggat gtgcttggca ctgcgccagt ccagggggag
     1441 gacgggcagg tccctcttgt gagcctcgct gatgacctta cccgagaagg cgtgactgac
     1501 atacgacgtc agcacgacca caaggcgggt cgtcgcaagg acagacggat ccaggtcctc
     1561 cttgccgttt gtagcaatgc agacactgtt gggaagccac gtctgcagat ttcgagacag
     1621 ggtctcatgg cctccggcta ccaggatccg ctcagtgatg gggtgtaccg tgtactgcac
     1681 ctcggcctca ggctcggcgc gggccgcaag gctgcccatc aggcgggagt tttccgcatc
     1741 ccgcgcactc aggcgttcgc ggaccttgtc cagctccccg gtcagccggc gtacctcccg
     1801 caactgagtc acaaggtctc cacggacctt gcgaagatcc tcgtcgtcat cctcggcgtc
     1861 gactgccaga cttgacgcgg gtacaggcaa cgactgcttc gcgggcgctc gccgcaaggc
     1921 aaagaggccg gcaagaatgc cgaccgccgt gccgtcgtca agccccacat ctgtgccggc
     1981 cagcagaaac gcagcctgcc agacgtcacc cggacccggg ctgtcaggct ggctcatctg
     2041 cgctacctgc atg
//
LOCUS       DATMZD010000177         2244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_35882,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000177 DATMZD010000000
VERSION     DATMZD010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35882"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="VN478_03600"
     CDS             complement(<1..522)
                     /locus_tag="VN478_03600"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmG family class I SAM-dependent
                     methyltransferase"
                     /protein_id="HWQ21791.1"
                     /translation="MNTADTSNATELLPLVRESMMDFSRVLSPANRPAVCTDAFCTGL
                     ASFLALLARANEVMNLTSDADPSHLLATHVRDSLAPLIVGLESPHSLLDIGTGGGFPV
                     VPLALAWPTARATMTESIGKKARFLESLLAGVPLPGSRVIHGRVEDPVSALPQRAFDM
                     ITARGVAHVATVLQ"
     gene            complement(564..1037)
                     /locus_tag="VN478_03605"
     CDS             complement(564..1037)
                     /locus_tag="VN478_03605"
                     /inference="COORDINATES: protein motif:HMM:NF018410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HWQ21792.1"
                     /translation="MPILVRNILHIFLITIIPGVEVRGALPYAIGVLKMPVWEALLVA
                     TLANLCIAPLFYLLEKPVLTIASRWAWFQRYRSRLAIRSKSSLARYGLFIGLALFVAI
                     PLPGTGAYTGCLIAEIARMKKPLAIASISLGVLGACTVVFLASVGVIKGVLAKWF"
     gene            1146..1766
                     /locus_tag="VN478_03610"
     CDS             1146..1766
                     /locus_tag="VN478_03610"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HWQ21793.1"
                     /translation="MLEGKFGNCQVDEVLQTIVQGKGTGRLKLEGTSIFGGRVQASFF
                     IEDSRIVHVDVGPGTAYTSLVDLFSLREGAFSFAGGETTGARDQSVSVADIVLQVTAA
                     LDEWNSMRQRLGSLDAVYALRADGATKDLTLTREQWQIMASLDGKMSMREIARKTGKG
                     SVVVSKALYGFMEMGLTSEVEAVPVSQDEERGQPQRRGFLGLWSRK"
     gene            1775..>2244
                     /locus_tag="VN478_03615"
     CDS             1775..>2244
                     /locus_tag="VN478_03615"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: protein motif:HMM:NF012690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HWQ21794.1"
                     /translation="MPGLREGLFFDDVMLLPRRSEVSASDVSLESKLATNVTLRSPFV
                     TSTREPAADYRLASTVARVGGIGVIPLLASVEDQAHEVRKVKELMCKEEGAACDMKGR
                     PVVGALVDLEREGVLEWIGSLAEVWADIIVVDVPHADRLTILRQIQAVKAEYDWL"
BASE COUNT          481 a          624 c          743 g          396 t
ORIGIN      
        1 ttgcaggaca gtcgcgacat gagcgacgcc cctcgcggtg atcatgtcaa aggcccgctg
       61 gggcagtgcg cttacggggt cctcgacgcg cccatggatc acgcgcgagc ccggcagggg
      121 cactcctgcg agcaagctct ccaggaagcg ggccttcttg ccgatcgact ccgtcatggt
      181 ggcccttgcc gtcggccagg cgagtgccag cgggacgacc ggaaaccccc cgcctgtccc
      241 gatgtcgagc agggagtgag gggactcgag ccccacgatc aggggagcaa gcgagtcgcg
      301 gacgtgtgta gccagcaggt gtgacggatc agcatcagaa gtgaggttca tgacctcgtt
      361 ggccctggcg agcagtgcca ggaacgacgc cagccccgta cagaaggcgt cggtgcagac
      421 ggcaggtctg ttcgcgggag acaggacgcg ggaaaagtcc atcatcgact cccgcacgag
      481 agggagaagc tctgtcgcat tcgacgtatc agcagtattc acagcattct ccagttactg
      541 ttccacgtgg aacaattccg cactcagaac cacttcgcca gaacgccctt gatgacaccg
      601 actgacgcaa ggaacacgac agtacatgcc cccaggacac ccagtgagat ggaggcaatg
      661 gccagaggct tcttcatgcg cgcgatctcc gcgatgaggc atccggtgta ggcgccggtc
      721 cccggcagcg ggatggccac gaagagggca agcccgatga agagcccgta cctggccagg
      781 gacgacttac tgcggatggc gagtcgcgag cggtagcgtt ggaaccatgc ccatcgcgac
      841 gcaattgtca gaacaggctt ctccaacagg tagaacaagg gagcgatgca cagattggcg
      901 agtgtcgcca ccaacagcgc ctcccacacc ggcatcttca gaacgccgat ggcatagggc
      961 agcgcgcccc ggacctcgac gcccgggatg atcgtgatga ggaagatatg caggatgttt
     1021 cgtacgagta tgggcactct ctcagtatac tggagggctg cgaggatgca acgagaacgt
     1081 tgaaaaaacc catgcaccac gatacaatga agaagtaacc tacgctgtgg gtccgggggt
     1141 caacgatgct tgaggggaag ttcggcaact gccaggtgga tgaagtcctc cagacgatcg
     1201 tacagggaaa ggggaccggc cgcctcaaac tcgaggggac gtcgatcttt ggcgggcgtg
     1261 ttcaggctag cttcttcatc gaggattcgc ggatcgttca cgtcgatgtc ggccccggaa
     1321 cggcgtacac ctcgctcgta gacctgttca gtctgcgaga gggagccttc tcttttgcag
     1381 gaggggagac gaccggggca agagaccagt cggtgtcggt tgcggacatc gtccttcagg
     1441 tgacagcggc tctcgacgag tggaattcga tgcgtcaacg acttggttcc ctggatgcgg
     1501 tatacgcatt gcgcgcagac ggcgccacga aagacttgac gctcacaagg gaacaatggc
     1561 agatcatggc gagcctcgac ggcaagatgt caatgcgtga gatcgccagg aagaccggca
     1621 aggggagtgt cgtggtgtcg aaggccctct acgggttcat ggagatggga ctcaccagtg
     1681 aggtggaagc cgtgccggta tcacaggatg aggaacgggg acagccacag cgccgtgggt
     1741 tccttggcct ctggagccgg aagtaggggg cgggatgccg ggactgcgcg aaggtctgtt
     1801 ctttgacgac gtcatgcttc ttccgaggag gagcgaagtt tctgcgtccg acgtcagcct
     1861 cgaatcgaaa ctcgcaacca acgtcactct gcgatcgccg ttcgttacct cgacgcgtga
     1921 acctgctgcc gactatcgtc tggcctcgac cgttgctcgt gtgggtggca taggcgtcat
     1981 ccctctgctg gcgagcgtcg aggaccaggc tcatgaggtg cgcaaggtca aggaactcat
     2041 gtgcaaggaa gagggagcgg catgtgacat gaagggacgt ccagtggtgg gcgctctcgt
     2101 cgacctggag cgggaaggag tactggaatg gattggctcg ctcgctgaag tctgggccga
     2161 catcattgtc gtggacgttc ctcatgcgga ccgcctgacc atcctccgcc agattcaggc
     2221 cgtgaaggca gagtatgact ggct
//
LOCUS       DATMZD010000178         2129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_85609,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000178 DATMZD010000000
VERSION     DATMZD010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85609"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..772)
                     /locus_tag="VN478_03620"
     CDS             complement(<1..772)
                     /locus_tag="VN478_03620"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HWQ21795.1"
                     /translation="MTGEIVWLLVLLALSFLFSMVEYGYVTITPMTLERRESHEGIAE
                     RLRSRMASNHSMLISVIILGNNVANVGASFLFARIVLSSFTQVDASLLSVVSTLVLTV
                     VVVLFSETTPKTIGKAFPDQTTLYTAPLLYPIYLLLYPGAWLLSVASGVLTKPLLKHA
                     KHSGYLSDSEDFRYLLKMGQEDGVIDKEEERLIYNIFDYTNTLAYEIMTPFVDVTTVE
                     KDSPITEVIQAIDKTGHSRLPVMDDDNVVGLVYAKDVLL"
     gene            complement(772..1281)
                     /gene="ybeY"
                     /locus_tag="VN478_03625"
     CDS             complement(772..1281)
                     /gene="ybeY"
                     /locus_tag="VN478_03625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HWQ21796.1"
                     /translation="MKEPDLSVHCRFELSDPGWRVDTKALTPLLVRVIQSIQPELSAI
                     RGVRHAVGVSCYFVQAAQMKRLNREHRGIDSVTDMLSFPLGYAAPGTGWVLGDIVICM
                     DAVESKAVSAGNEIEDQLVFSLVHSVLHLVGFDHAREGDRSLMERREEEVFAQLPQGL
                     RCHGVARRS"
     gene            complement(1268..>2129)
                     /locus_tag="VN478_03630"
     CDS             complement(1268..>2129)
                     /locus_tag="VN478_03630"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HWQ21797.1"
                     /translation="IYGALAALSEQRLSVIAANVGYLAAGGVIAAVFALGLTLVYERL
                     FNVATIMRLRELLDTGSKLLAQLFESAPGTYHHSLNVANLAAGAAQRIGANWLMARVG
                     GYYHDIGKLLHPQFFGENRGELPNIHEDIPPELSTKVILEHVQAGVTIAGSNHLPAEV
                     LDIIAEHHGTTVVQFFYDKASADQANLSPDTFRYQGPKPHSKESTLVFLADGVEAAAR
                     SLARVDKPALEGLIDTIVSARIADGQLAESQLTLGEIAAVKEFFLTSLISFYHIRDAY
                     VTPEEANERT"
BASE COUNT          483 a          604 c          606 g          436 t
ORIGIN      
        1 acaggaggac gtccttcgca tagacgagtc ccacgacatt gtcgtcgtcc atgactggga
       61 gacgagagtg accggtcttg tcaattgcct ggatcacctc ggtgatgggc gagtccttct
      121 cgaccgtcgt cacgtctacg aacggtgtca tgatctcgta ggccagtgtg ttggtatagt
      181 caaagatgtt gtagatgagc cgctcttcct ccttgtcgat caccccatcc tcctgcccca
      241 tcttcagcag atagcggaag tcttccgagt cgctcagata gccggagtgt ttggcatgct
      301 tcagaagcgg cttggtcagg acgcccgaag cgacggagag cagccaggcg ccaggataca
      361 acagcaggta gatggggtag agcagaggtg ccgtgtacag cgtcgtctgg tcagggaacg
      421 cctttccaat agtctttggc gtggtctcgg agaacagaac gaccaccacg gtaagtacga
      481 gggtcgacac gactgacaag aggctggcgt ccacttgcgt gaatgagctg aggacaatgc
      541 gtgcgaacag gaacgatgcc ccgacgttgg caacattgtt ccccagtatt atgaccgaaa
      601 tgagcatgga gtgatttgag gccatccggc tgcgcaagcg ctcggcaatt ccttcatgcg
      661 actctcgtcg ctccagggtc atcggcgtga tggttacata tccgtactca accatcgaga
      721 agagaaacga cagcgcaagg agaaccagta gccagacaat ctccccagtc atcaggacct
      781 tctggcaacg ccatggcagc gcagcccctg cggaagttgg gcgaagacct cttcctcacg
      841 gcgttccatg agagagcgat cgccctcccg ggcatggtcg aacccgacaa ggtgcagcac
      901 ggagtgcaca agggagaaga ccagctgatc ttcaatctcg ttgccggccg agacagcctt
      961 gctctcaacc gcatccatgc agatcacgat gtcccccagc acccatccgg taccaggagc
     1021 ggcatatccg agaggaaagg agagcatgtc agtgactgag tcgatgcctc tgtgctcacg
     1081 gttcaacctc ttcatctggg cggcctgtac gaagtaacat gagacgccaa ctgcatgtcg
     1141 aactccccga atcgcgctga gctcgggctg gatcgactgg atgacacgga caagaagcgg
     1201 agtgagtgcc ttcgtgtcga ccctccatcc gggatccgac agttcaaatc gacagtgtac
     1261 cgataggtca ggttctttca tttgcctcct cgggcgtcac atacgcatcg cgaatatggt
     1321 agaacgaaat aagggacgtg aggaagaact ccttgacagc tgcgatctcg cccagcgtga
     1381 gctgcgactc tgcaagttga ccatcggcga tgcgagcact cacgatcgta tcgatgagcc
     1441 cttccagggc tggtttgtcc accctggcaa gtgatcgcgc cgccgcctct acaccatcag
     1501 ccaggaagac aagggttgat tccttggaat gcggtttggg tccctgataa cggaaggtat
     1561 caggcgacag gtttgcctgg tcggcagacg ccttgtcata gaagaactgg acgacagtcg
     1621 tgccatggtg ttcagctatg atgtcgagca cctccgcagg caagtgattg ctgcctgcaa
     1681 tcgtcactcc ggcctgcacg tgctcaagga tcaccttcgt actgagttcg ggagggatgt
     1741 cttcatgaat gttcggcagt tcgccccggt tctctccgaa gaactgtgga tgcaggagct
     1801 tgccgatatc gtggtagtag ccaccaacac gtgccatcag ccagttcgct ccaatacgtt
     1861 gagcagcgcc cgccgcaagg ttcgccacgt tcaggctgtg atggtacgtc ccgggagcgc
     1921 tctcaaacag ctgcgccaga agcttgctgc cagtatcaag aagctctcgc agcctcatga
     1981 tcgtcgctac gttgaacagg cgctcgtaga caagggtcag tcccaatgca aacacggcag
     2041 caatgactcc tcctgctgcc agatatccga cgttggcggc aatcacagac agtcgctgtt
     2101 cactcaacgc ggcaagagct ccatatatg
//
LOCUS       DATMZD010000179         2024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_136346,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000179 DATMZD010000000
VERSION     DATMZD010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136346"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..585)
                     /locus_tag="VN478_03635"
     CDS             complement(118..585)
                     /locus_tag="VN478_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013931446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HWQ21798.1"
                     /translation="MEEIRRIAVGDIAPDFTLNDNRKRPIHLADYRGKKVLLSWHPLA
                     WTKVCAEQMKALEAHLADFSRLNTVALGLSIDPVPSKNQWAKQLLIAETMLLSDFWPH
                     GGVARAYGLFRDVEGFSERANVLIDASGRVTWVKVYGVPELPDINEVLVRCAD"
     gene            complement(752..1429)
                     /locus_tag="VN478_03640"
     CDS             complement(752..1429)
                     /locus_tag="VN478_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009169943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HWQ21799.1"
                     /translation="MDEKMQKVREAVERELVCSAHSMAHVWRVYRLCLRLAADETNVD
                     TEVLELASLLHDIARVREDTDDTGMTDHAVLGAAMAEPILRDAGYPEATVRHVCSCIA
                     THRFRGDAVPATIEAKLLFDADKIDSLGAVGIARAYMLAAGYGEAIYSDENVADYVAC
                     NHVGGVATGRIRVLSEHTPNLEFEIAFKRIPDRLFTPQAHSVAVDRLAVMQTFFDRLK
                     AELDGLA"
     gene            complement(1457..1894)
                     /locus_tag="VN478_03645"
     CDS             complement(1457..1894)
                     /locus_tag="VN478_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006834986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWQ21800.1"
                     /translation="MAESDELSQAGLLHHVEIYCTDLKTKTDFWGWFLGLLGYSVYQD
                     WPGGRSYRLGPTYLVFVQAEQRFCGEPYHRCHPGLNHLAFHAASRRQVDEVTELLRGR
                     GVRVLYEDRHPHAGGPDCYAVYFEDPDRMKVELDAPDEDAQST"
BASE COUNT          392 a          642 c          564 g          426 t
ORIGIN      
        1 cttgcagcac gtgccaggtc ccagatcagg tcgtcagtga tcgctaccgg atccagagaa
       61 cgatatgccc tgcgggcctc gatggcgttc ttgacatcca tggatgtctg cacgcgatca
      121 atcggcacag cgcacaagta cttcgttgat gtcgggcagc tctggaacgc cgtagacttt
      181 cacccaggtc actctgcctg acgcgtcgat gagcacgttg gcacgctccg agaacccctc
      241 cacatcgcgg aacagcccat atgcgcgcgc cacgcccccg tgaggccaga agtcggacag
      301 cagcatggtc tcagcaatga gaagctgctt cgcccactgg ttctttgacg gaaccggatc
      361 gatgctgaga ccgagcgcga cagtattcaa acgcgagaaa tccgcgaggt gagcttcgag
      421 tgccttcatc tgctcggcgc agactttggt ccatgcgaga gggtgccagg agagcaggac
      481 cttcttgcca cggtagtccg ccaggtggat gggacgcttt cggttgtcgt tcagagtgaa
      541 atccggcgcg atgtcgccga ctgcgattcg tctgatctct tccatatgtt cctcctgtgc
      601 ctgctgtcgg ttactcaagc ggccggcctt gttcctagta tgtccgtctg catcgcatgt
      661 gtcaatgcga caagtcagcg gtccctgcag ttccgtcaca gtctcgatgc aggctccagg
      721 tttgccgtct ccacaggtcg accggattct gtcaggctag cccgtccagt tcggccttga
      781 ggcggtcgaa aaatgtctgc atgacagcga gccgatcgac ggcgacggag tgcgcctgcg
      841 gtgtgaagag gcggtcgggg atgcgcttga acgcgatctc gaattcgagg ttgggcgtgt
      901 gttcgctcaa caccctgatg cgccccgttg ccacgccgcc aacgtgattg caggcgacat
      961 agtcagcgac gttctcgtca ctgtagatcg cttcgccgta tcccgcggcc agcatatatg
     1021 ccctggcgat gccgactgcg cccagagaat cgatcttgtc tgcgtcaaac aggagcttgg
     1081 cctcaatggt ggccgggaca gcgtcacccc tgaatcggtg tgtggcaata caggaacaca
     1141 cgtgccggac cgtcgcttcg ggataccctg catcacgcag gatgggctcg gccatcgccg
     1201 ctcccagaac ggcatggtcg gtcatgcccg tgtcgtccgt gtcctcccgt acccttgcga
     1261 tatcgtggag aagtgaggcc agttccagga cttcggtatc gacgttcgtc tcgtccgcgg
     1321 caagcctcag acaaagacga tacacacgcc atacgtgggc catactgtga gccgaacaga
     1381 ccagctcacg ctcaactgcc tcgcggacct tctgcatctt ctcgtccata ccggttcctg
     1441 cctccccgtt ccacatctac gttgattgtg cgtcttcgtc cggcgcgtcc agctcgacct
     1501 tcatgcggtc agggtcctcg aagtagaccg cgtagcagtc gggccctccg gcatgcggat
     1561 gccgatcctc gtacaaaaca cgcactcccc gacccctcag caactctgtc acctcgtcga
     1621 cctgccgccg cgaagcggca tggaatgcga gatggttgag accgggatga cagcgatggt
     1681 acggttcgcc acagaaccgc tgttcggcct gcacaaagac cagatacgtc ggccccagcc
     1741 ggtaactccg tccacccggc cagtcctggt agaccgagta gccaagcaga cccaggaacc
     1801 atccccagaa gtccgtcttt gtcttcaggt cagtgcagta gatctcgaca tggtgcagca
     1861 atcctgcctg actcaactcg tccgactcag ccatgaaacc tccctgcgcg gccgacaatg
     1921 cggtccacgc tctcttattc attatgactg tctgaactat cgctgcaaac ggttgtcctg
     1981 ctatactgca ttcatgaaac gcccggacaa cgcttctctt atcg
//
LOCUS       DATMZD010000180         4074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_25179,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000180 DATMZD010000000
VERSION     DATMZD010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25179"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="VN478_03650"
     CDS             complement(<1..271)
                     /locus_tag="VN478_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RND transporter"
                     /protein_id="HWQ21801.1"
                     /translation="MGRGNVRVALVVLLVVAAVGLAGCRLASRLQQAGSTAGPDTEYI
                     VTIQDVASGTSVSGTIHPTRSAALSAQAQAKVTAVYVKEGDPVTAG"
     gene            complement(641..1900)
                     /locus_tag="VN478_03655"
     CDS             complement(641..1900)
                     /locus_tag="VN478_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21802.1"
                     /translation="MRRIVLALLALIVAVSMVGGMPAVTSAATPYVELALTSGYIGDY
                     DEYHVTFLLNQPVSKGSTIAIGFDKSVNHSPMRVIATGDVLLDGVACSSVSWSGHTMT
                     VTAQSDLIADAEHTIVIQTGAMVQNPQTAMGIRVTLSDTVHGILLTSNYCMVNTSSAI
                     AGISMSAESTGSSGLTVSVRFRTGRNGSLKGTAATWSSVTSLASYNDTLTLRLSNTLS
                     KLWAESDWPTVSLSTPPYGIGARQLRIVSTTIRNEDDPATSQRIMTFIPDVDIPASTE
                     VLLTIELDGVSLKKPLTTDDYVAVWTSKEPTMVTIRPGDGGEPTTPGSTTEPADTAAP
                     TVTWTSRASTLLPRLVTLNINVTEVNLDEAWFSGGTDSLIHTRLAAGDNAIMLVNRTG
                     IHGTIVATDKAGNTTIVPVDLPAPSMT"
     gene            complement(1910..3136)
                     /locus_tag="VN478_03660"
     CDS             complement(1910..3136)
                     /locus_tag="VN478_03660"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWQ21803.1"
                     /translation="MDVRDSFKNATRAIMAAKMRSFLTMLGIIIGIAAVVMLMGVGNG
                     SKQSILSRMQGSGSGNLYLTAGTRANVLMGGFGGQGGRPGQSSSSTKITMADLTYIED
                     NSTMSIIVSPVNTTNLTIKRNSTTMSGSGIFATTNYFEVEGVTIADGRAITQGDIDNR
                     TRNVVIGDTVATTLFPDESAVGQDIRLNGATYKVVGIATAKGETMGSSVDTSIILPFS
                     VGEKSFGITRNVVSRTTIKCVDPTQSAAVTAEITDNLTRKHKKVDFTVTDVAAMTELV
                     TSSMTTLTTLLAAIGGLSLLVGGIGIMNIMLVSVTERTREIGIRKALGAKEWDILGQF
                     LIESSLLSISGGAIGILISVLGSEFVVSRFTTTAITGSSIALAVGFSIAVGVFFGVYP
                     ARKAARLKPVEALRYE"
     gene            complement(3138..3824)
                     /locus_tag="VN478_03665"
     CDS             complement(3138..3824)
                     /locus_tag="VN478_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006862225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWQ21804.1"
                     /translation="MSLIQFSNIRKAYSIGGVTIEALAGVTLDIGEKEFVAIMGPSGS
                     GKSTLMHILGCLDSPTSGEYVLNGKDVSKLRADDLARVRNRDMGFIFQAYNLLPQMSA
                     LENVLLPCSYADQGLRAKEVRARALELLERVGLSNRMHNRPVEMSGGEQQRVAIARSL
                     MMNPPILVADEPTGNLNSKGAREILDIFKELHDEGKTIIYVTHDDNIGAKAERVIRIR
                     DGLVVADEGD"
     gene            complement(3828..>4074)
                     /locus_tag="VN478_03670"
     CDS             complement(3828..>4074)
                     /locus_tag="VN478_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21805.1"
                     /translation="VTTSRVQVKTGLEGDEYVEITSGIKAGDRILRKASTTATTTTTS
                     TTPSSTQSDTPGILGGITGGAGGPPDGGAGGTPPGGN"
BASE COUNT          762 a         1244 c         1170 g          898 t
ORIGIN      
        1 cccctgccgt gaccgggtct ccctccttca cgtagacggc agtcaccttg gcctgggcct
       61 gtgcgctcag cgccgccgac cgggtcggat gaatcgttcc cgagaccgac gttccggaag
      121 ccacgtcctg tatcgtcact atatactcag tgtccggacc agcggtgctt cctgcctgtt
      181 gcaggcggct tgccagtctg catcccgcca gcccaaccgc ggcgaccacc aacagcacga
      241 ccagggcgac acgcacgttc cctcttccca ttgttcctcc gtcagggaca ccgtcccgtc
      301 tcgtcatcct tctccaacct gcagaccggt tccacgagcg ccagcctgca gcggccagct
      361 tcgaccctgc acaactctcg cgcctgacga cagacaagcg cccttccgcg ttgctctgcg
      421 ggtatcgcca attatagcag ctatgcgcgc gtcatgttga accagtcgag ccttcggcac
      481 ggcatgtgag actgaggcac agacccgctt cacaggatgc cctggctgca ttgtgaaggg
      541 ccgcagctgg tccggccttg ttcacgccgg gccgctcacg gcccttcgga gagaagtatc
      601 tacttgttgt gcaggcggtt ggtgtgctcc cgccgaaaca tcacgtcatg gacggagcag
      661 ggaggtcgac aggcacaata gtcgtattgc ctgccttgtc agtcgccacg atggtgccgt
      721 ggatacctgt ccggttgacc agcatgatgg cgttgtcgcc agcagccagc cgggtgtgga
      781 tgaggctgtc tgtgcccccg gagaaccagg cctcatccag gttcacttcg gtgacattga
      841 tgttcagcgt cacaaggcgt ggcaggagcg tactggccct ggaggtccag gtcaccgtcg
      901 gcgccgccgt gtctgccggc tctgttgtcg accctggtgt ggtcggctcg ccgccatcac
      961 cgggtcgaat cgtcaccatc gtgggctcct tcgaggtcca caccgcaacg tagtcatctg
     1021 ttgtcaacgg cttcttcagg ctcactccgt cgagctcgat ggtcagcagg acttccgtcg
     1081 atgcaggaat gtccacatcg gggatgaatg tcatgatcct ctgagatgtc gcgggatcat
     1141 cctcgttgcg aatggtcgtc gagacgatcc tcagttgtct cgcccctatc ccatagggag
     1201 gagtgctgag cgagacggtc ggccagtcac tctcagccca gagctttgac agtgtgttgg
     1261 agagcctgag ggtgagcgtg tcattgtagc tggccagaga cgtaacggac gaccacgtcg
     1321 cggcggttcc cttcagagaa ccgtttctgc cggtcctgaa gcggacactg acagtgagtc
     1381 cacttgaacc cgtgctctcg gccgacattg agatgcccgc aatagcgctc gacgtgttga
     1441 ccatgcagta gttggacgtg agcaggatcc cgtggaccgt gtcggaaagc gtcaccctga
     1501 tgcccatggc tgtctgcggg ttctggacca tggctcccgt ctggatgacg atggtatgtt
     1561 cagcgtccgc tatcaggtca gactgcgcgg tcacagtcat cgtatggcct gaccagctca
     1621 ctgatgaaca ggcaacccca tccagcagga cgtccccggt cgcgatcacg cgcatcgggc
     1681 tgtgattcac tgacttgtcg aacccaatgg cgatcgtgga gcccttcgac actggctggt
     1741 tcaggagaaa tgtgacatgg tattcgtcgt agtccccgat atagccgctc gtgagcgcga
     1801 gttcgacata tggagtcgca gcagatgtta ccgcgggcat ccctccaacc atggatactg
     1861 ctacgatgag agccagcagc gccagtacta ttcttcgcat agcgtcctcc tattcataac
     1921 gcaacgcttc gaccggtttc agtcgagcgg ccttgcgggc ggggtagacc ccgaaaaaca
     1981 ctccgacggc gatggagaag ccgacagcaa gagcgatcga agagccggtg atggccgtgg
     2041 ttgtgaagcg ggacacgaca aactccgaac ccagaacgct tatgagaatt ccgatcgctc
     2101 cccctgagat gctcagcaga gacgactcga tgagaaactg gccgaggata tcccactcct
     2161 tggcgccaag cgcctttcgg atgcctatct cacgtgtacg ctctgtgacc gagacaagca
     2221 tgatattcat gattccaatg ccgccgacca gcagtgagag tccaccgatg gctgcgagca
     2281 gggtcgtcag cgtcgtcatg gacgacgtca ccagttctgt cattgccgcc acgtccgtaa
     2341 ctgtgaagtc caccttcttg tgcttgcgtg tcaggttgtc agtgatctct gccgtcacgg
     2401 cggcgctctg cgtggggtcc acgcacttga tcgtcgttcg cgatacaacg ttgcgggtta
     2461 ttccgaacga cttctcgccg actgagaacg gcaagatgat cgacgtgtcg accgagctgc
     2521 ccattgtctc acctttggcg gtggcaatgc cgacgacctt gtaggtagcg ccgttcagcc
     2581 tgatatcctg tccgactgca gactcatccg ggaagagtgt cgtggcaact gtgtcgccga
     2641 tgacgacgtt ccgcgtccgg ttatcgatat ctccctgtgt gatcgcccgt ccatcagcga
     2701 tagtgacacc ctcgacctca aagtagttcg tggtcgcgaa gatacctgag ccggacatcg
     2761 tcgtgctgtt ccgcttgatg gtgaggtttg tcgtgttcac aggagagacg atgatcgaca
     2821 tcgtggagtt atcctcaatg tacgtcaggt cagccatcgt gatcttcgtg ctcgatgacg
     2881 attgaccggg acggcctccc tgccctccga aaccacccat gaggacattt gccctggtgc
     2941 ccgcagtcag ataaagattc ccggaacccg acccctgcat tcttgagagg atactctgtt
     3001 tcgatccatt gcctactccc atcagcatga cgacggcagc gatgcctatg atgataccga
     3061 gcatcgtgag gaacgagcgc atcttggcgg ccatgatcgc tcgtgtggca ttcttgaagc
     3121 tatctctgac atccatgtca gtccccctcg tctgccacga cgaggccgtc gcgaatgcgg
     3181 atcacgcgtt ccgcctttgc accgatattg tcgtcgtggg tcacgtagat gatcgtcttc
     3241 ccttcatcat gcagttcctt aaagatgtca aggatctctc gggctccctt ggagttcagg
     3301 tttcccgtgg gctcatccgc cacgagaatc ggcgggttca tcatgagaga ccgggcgata
     3361 gccacgcgct gctgttcgcc gccggacatt tctaccgggc ggttgtgcat acggttcgac
     3421 aatcctaccc gttccagaag ttccaaggcg cgcgctctga cctccttggc cctcaagccc
     3481 tgatctgcgt aggaacaggg cagcagcacg ttctccaggg cgctcatctg gggcagcagg
     3541 ttgtatgcct ggaagatgaa ccccatgtcg cggttgcgga ctctggcaag atcatctgcc
     3601 ctcagtttgg agacgtcctt gccattcagc acatactcac cggatgtcgg gctgtcgaga
     3661 cacccgagga tgtgcatgag agtagactta cctgaacccg acggacccat gatcgccaca
     3721 aattccttct ccccgatgtc cagcgtcacg ccggcgagcg cttcgatggt cacgcctccg
     3781 atggagtagg ccttgcggat gttgctgaat tgaataagac tcatgagtta gttgccaccg
     3841 ggaggcgtgc cacctgctcc accgtcgggg ggaccgccag cccctcctgt gatgccgcca
     3901 aggatgccag gcgtgtctga ctgcgttgag ctgggagttg tcgatgtagt cgtcgtggtc
     3961 gcagtagtgc tggccttgcg caggatcctg tcgcctgcct tgatcccgga cgtgatttct
     4021 acgtactcat ccccttcaag gcccgtcttg acttgcaccc tgctcgtggt gacc
//
LOCUS       DATMZD010000181        13176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_75020,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000181 DATMZD010000000
VERSION     DATMZD010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 13176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13176
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75020"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VN478_03675"
     CDS             <1..221
                     /locus_tag="VN478_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21806.1"
                     /translation="NMGVRSAASVVKALAAGAAAVVLNSLTPDTAARVTTDIARQVRQ
                     SLSYVGARSIMELREKAEFIRIRPPSAT"
     gene            complement(305..1765)
                     /locus_tag="VN478_03680"
     CDS             complement(305..1765)
                     /locus_tag="VN478_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012543982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWQ21807.1"
                     /translation="MRDQNRTPFFDAVQSYIAKHRAGLHMPGHMGGQGIHPRLKEFWG
                     ENVFLSDLTEVEGLDYLHRPIGVVKEAQELAAEAYGAIESFFLVNGSTVGNQAMILST
                     CDPNDRVIVQRNSHRSMYGALILNELRPIYLEAEEDRLLQFQTFPSKKTFARVLRDHP
                     EAKTVFLTNPNYFGISSDIDYYVQKAHATGRRILVDEAHGSHFHFHPDLPKSAVDAGA
                     DMVVQSTHKTLSGLTQTSMLHLGSRRVDKELVRATLTMLESSSPSYILMTALDVARMQ
                     MATNGHDLLQRAIDLAEEARRRVNAIEGLFAPGRERARSDAVFDVDLTKLTIHVSRLG
                     LTGFQVEEILNRDYNVEIELSDLENILAFITIGTPREAVDILIKALEDISRKRHGKTS
                     TITPPPPIPDIPEMVLFPFEAYYAKRERVRMEDSVGRIIAELVTPYPPGIPITVPGEV
                     MTHECVDYMVFMRDHGADLQGIIDKTARYVQVVKER"
     gene            complement(1838..2530)
                     /locus_tag="VN478_03685"
     CDS             complement(1838..2530)
                     /locus_tag="VN478_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HWQ21808.1"
                     /translation="MLHEQLTYLGHSAFLITTPAGEILIDPFLSGNPLAAVDPDDVDP
                     DYILVTHGHADHLGDTIPIAKRTGATVVGPAELITYVAGKGVSKVHPMHIGGSHTFPF
                     GELILTQALHGSSVTENGHLVYTGNPCGFVIKTGERAIYHAGDTGVFLDMELIGRLHP
                     LDVALLPIGGNFTMGIDDAVEAVKMLAPRTVIPMHYGTFPLIDVDPQVFRTKVESSTS
                     ARCMVLAPGQTF"
     gene            2715..3644
                     /gene="thrB"
                     /locus_tag="VN478_03690"
     CDS             2715..3644
                     /gene="thrB"
                     /locus_tag="VN478_03690"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HWQ21809.1"
                     /translation="MINKVTVSVPASTANLGPGFDAFGLALEMRSTYTAEILSSDEKW
                     SVHFDGMGADVLNEKGSKFVSEVMDYFERAESVHLPPISISVHTGIPLTRGLGSSASA
                     IVGLLSCFEVLMNTKFDTGKVLRYAIAIEGHPDNVTASLVGGFTIAASDKSEVAYMKV
                     VPKDLKVIVTVPSSFLSTKKARAVLPDVYARKDAVFNIGRASMLSACIFSGNYDLLSF
                     ALHDRLHEHYREKLVTGFREVSHAADRAGAYGSFLSGAGPSIAALAEPAKAEKVAKAM
                     QKAMIELDAEAETFVLDIAAQGVILKTIDDEEA"
     gene            complement(4099..5571)
                     /locus_tag="VN478_03695"
     CDS             complement(4099..5571)
                     /locus_tag="VN478_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HWQ21810.1"
                     /translation="MAVFDMTVHEDVLATTVKLAQDRNIIIPTFAQQKDPSLVPDKVK
                     AKLKGVGLWDVNPINLFRITWHNDVKTGLYGDVNYIELPSSITGIKARVVGIVGKYFP
                     TGAHKVGAAFGCLVPRLVTGQFDPTCQAAVWPSTGNYCRGGAFDCALLATDAVAILPE
                     GMSKERFDWLREIGTKEVIGTPGTESNVKEIYDKVHELKTTRGDAIVVFNQFEEFGNA
                     LWHYDMTGSAFEQVFNSIKGPRSRMAGYVSATGSAGTISAGEYLKKVYPYSHITAVEA
                     LQCPTLYANGFGAHRIEGIGDKHVPWIHNVRNTDAVAAIDDDYPMHLLRLFNEEAGKA
                     YLEKKGVPRAIIEQLNLMGISSLANMVAAIKMAKYEEMDENDIVFTVFTDSADLYLSR
                     LEELRAEYGPYTEMDAALDYAKYLEGILTDWYRELNYRDRKQLHNLKYFTWVEQQGKT
                     AEELRELWEPEFWDAIWTAAPEFDRLINQFNEKTGLLAKL"
     gene            complement(5661..6380)
                     /locus_tag="VN478_03700"
     CDS             complement(5661..6380)
                     /locus_tag="VN478_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21811.1"
                     /translation="MDKLDVILLIGRPAAGKSETIDFLKQLPDEKRLQAYHIAPFEEL
                     DDFVYVWQTFENDAIRESMGLGRRDTDKALYFLDDRIWDFFIERIDLDLRKKLARDPL
                     LFERHTVMMEFARGGEHGFENAFQHLSDTVLAHAVILYIDVSFEESLRKNRRRYRPDQ
                     ADSILYHSLEDAKMERYYKDNDWACLSGGHDEGFITVRGHQVPFAVFHNEPEKTLDPE
                     LLGAALRDVTGRLVKLFIHEG"
     gene            complement(6517..7326)
                     /locus_tag="VN478_03705"
     CDS             complement(6517..7326)
                     /locus_tag="VN478_03705"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HWQ21812.1"
                     /translation="MEEHIGSATLLGHGRTAEVYAWKEDSVLKLFVPGFPSSLIDQEV
                     DGTRAANSTGIPSPQLRGVITVDGRRGLVLERLSGTSMLTLLGREPLRVTHYAGIFAE
                     LQAHMNDCHAPGLTSRREDLARRIARAGVVARVKGDALATLERLPDGDSLCHCDFHPD
                     NVLMTPQGPSVIDWSFAARGTPASDAARTALLLRIADAPPGSFRVAWVERETRNRFYR
                     AWLKRYRELQPDAGEAMWSWLLPVAVARLADGIPGEEPRLTALIEELQQKR"
     gene            complement(7362..8666)
                     /locus_tag="VN478_03710"
     CDS             complement(7362..8666)
                     /locus_tag="VN478_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWQ21813.1"
                     /translation="MVDQKLNESSAVECLDGQDPISERRFSLNNTFAAMRYPNYRIWF
                     AGQLVSLFGTWMQMTAQGYLAYQLTRSAAFLGYVAFANGLPVWLFMLYAGSVADRVPR
                     KTLMLYTQSVMMVLAFILAVLTFTGAVRPWHILVLSFLLGIANTFDSPARLALVNQLV
                     DDRRHLPNAVALNGSMFNLATALGPAAAGAVYALLGPAWCFTINGLSFVAVIAALLLM
                     RLRPVPLCPRSQGSTAGIVEGLRYVWRHEDIRSLILLIGVTSLFGLSFAALIPAWAVK
                     VLHGTSTTGATINGFLQSARGIGALLAALTIATVSHLRVKGRLATFGSFAYPIVLLVF
                     AIARSTGLALVLMGLVGFASILVVNLCNVLIQSQVEDSLRGRVSSVYSLVFFGMMPIG
                     SLLVGWLAQWTNEPVAVIVSACGALTGAILIWMLVPRLRSMG"
     gene            8851..9771
                     /locus_tag="VN478_03715"
     CDS             8851..9771
                     /locus_tag="VN478_03715"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HWQ21814.1"
                     /translation="MKKTIAVLLALSLAFFAGCGNPTPKVETGKVTITVTDGNGAPIK
                     GATVTLTDGDGKETTAASSSSGVAVFDALPYGEYDVASSMAGFADGTDTITVDSSSQD
                     VALDMTADTGDSTDSGQDTDAIGDVSVIDSLTSYRYKWTTLSEGDARGSVIEGGTEKP
                     NSEYSIVRDKEGKAQMEFYKVGDTLRMGTAGNWQTLSGDDAKDFGYGESFISTLSNDY
                     ADMKSNWSDWKKSDGGAINGYDTDKYTYSGNANGTTVTDTAYYIKSGEFKGVMTRYDI
                     SYQSDADATKRSGYTIDVYDLNKPLGIKLP"
     gene            10090..10698
                     /locus_tag="VN478_03720"
     CDS             10090..10698
                     /locus_tag="VN478_03720"
                     /inference="COORDINATES: protein motif:HMM:NF015716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HWQ21815.1"
                     /translation="MERKGFVLKGTSQSVEMIIGQVTPIGDILQQLDEVLARRGDIMG
                     RLPFALDTGDRQLSNAELAAFERFFIDHHLDYQLPQPEPAVDPSEVLFPALPSDPTIV
                     VDHTLRSGQSVTTGGNVLVVGDVNEGAEVHAAGNIYVLGVIRGVASAGESITALGFEP
                     SRMTVSDVEYQASERGFRDARKARTAFVENGEIHIEVAGKRR"
     gene            10703..11491
                     /gene="minD"
                     /locus_tag="VN478_03725"
     CDS             10703..11491
                     /gene="minD"
                     /locus_tag="VN478_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HWQ21816.1"
                     /translation="MGKVIVVTSGKGGVGKTTTTANVGGALTKLGKRVLLVDMDVGLR
                     NLDLVMGLENRVVYTFMDVLEGRCTADQAMVKEKHAGGSLMLLAASQLHNKQDLDLEK
                     VKNAIAGLAAEFDYVFLDSPAGIEHGFDASSAPAGQALVVVTPDVTSVRDADRVIGLL
                     ENKGVRDCGLIVNKYDRTLIERKKQLTSDDIVDVLGAELTGIVPEDKAIIDATNRGDI
                     LVLSQSTPAATAYLEIAHRLEGDETHFSALSTGRSLLSIFRRQK"
     gene            11488..11727
                     /gene="minE"
                     /locus_tag="VN478_03730"
     CDS             11488..11727
                     /gene="minE"
                     /locus_tag="VN478_03730"
                     /inference="COORDINATES: protein motif:HMM:TIGR01215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HWQ21817.1"
                     /translation="MSWFHKATNEIAKERLTFILVQDRSQLSPAVMEQMKEELVAVIG
                     KYFDIDKAQIELNVERQEGRQVVETSIPINGLRHQ"
     gene            11876..12379
                     /locus_tag="VN478_03735"
     CDS             11876..12379
                     /locus_tag="VN478_03735"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ family
                     protein"
                     /protein_id="HWQ21818.1"
                     /translation="MASGASAQPQGTLTLSPEQVHELLPQREPFLFVNGPAEVVPGVR
                     VSARATYAQDSSFYAGHFPGFPLTPGVIIIETMAQAASLMMLTMPGCSGHIVYFVGIR
                     EARFFRAVLPGAVVEIMGSVVSKRHGIVESGMEAWTNGLRAASAFVTCTLRPRRGSLD
                     VKEPSDE"
     gene            12454..12999
                     /locus_tag="VN478_03740"
     CDS             12454..12999
                     /locus_tag="VN478_03740"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010632881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HWQ21819.1"
                     /translation="MVAQHTGKNSMRIIMTVADRMWRGFLINWLAASPLLVNQTRASL
                     MRLYGIRTATHGIRPGCYFAGRDVVIGADTFVNYRCFFDGSAPIRIGRKCGIGMEVLF
                     CTSTHFLGPTTERAGPVNPQPITVGDGCWIGARSVILPGVTIGDGCVIAAGAVVNKDC
                     APNGMYAGVPARRTRELPVED"
BASE COUNT         2724 a         3801 c         4106 g         2545 t
ORIGIN      
        1 gcaacatggg cgtgcgctcc gcagcgagcg tcgtcaaggc tcttgccgcc ggggccgcag
       61 ccgttgtcct caattctctc acaccggaca cagctgcgcg ggtcacgacg gacattgcgc
      121 ggcaggtgcg ccagagcctc agctacgtcg gcgcgcgctc catcatggaa ttgcgcgaga
      181 aagccgagtt catccggatc cgacccccgt ctgccacctg atcccgcttc ttcccggaaa
      241 tgccagacgc cctgaccact acgtcagggc gtttttgcga gagaggaaca cgcgcagcga
      301 tcggtcagcg ttccttgacg acctgtacgt acctcgcggt cttgtcaatg atcccctgca
      361 ggtcggcgcc gtgatcgcgc atgaacacca tgtagtccac gcactcatgc gtcatgactt
      421 cgccggggac agtgatcggt atgcccggag gatagggagt cacaagctcc gcgatgatgc
      481 gcccgacgga gtcttccatg cgcactcgct ccctcttggc atagtaggct tcgaacggga
      541 agagaaccat ctcggggatg tccgggatag gcggcggagg agtgatggtc gaggtcttgc
      601 catggcgctt gcgggagatg tcctcgagtg ccttgatgag gatatccact gcctccctgg
      661 gtgtcccgat cgtgatgaac gccaggatgt tctcaaggtc ggacagttcg atctctacgt
      721 tatagtccct gttgaggatc tcctccacct ggaatccggt cagtccgagt ctggacacat
      781 ggatcgtcag cttggtgaga tcgacgtcga agacggcgtc gctgcgcgcg cgttccctgc
      841 cgggggcaaa gaggccctcg atggcgttga cgcgccgacg ggcttcttcc gcaaggtcga
      901 tggctcgctg aaggaggtcg tgaccatttg tggccatctg catgcgggcg acgtcgagag
      961 cagtcatgag aatgtacgac ggagatgagc tctccagcat cgtgagcgtg gcccggacaa
     1021 gctccttgtc gacgcggcgt gagcccagat gcagcatgga cgtctgcgtg agccctgaga
     1081 gcgtcttgtg tgtgctctgg acgaccatgt cggcgcccgc gtcgacagcg ctcttgggaa
     1141 ggtccgggtg gaaatgaaaa tgtgacccgt gagcctcgtc caccaggatg cggcgtcccg
     1201 tggcatgcgc cttctggacg tagtaatcga tgtccgaact gatgccgaag tagttgggat
     1261 tggtcaggaa cacagtcttg gcttctgggt ggtcgcggag cacgcgagcg aacgtcttct
     1321 tggacgggaa cgtctggaac tgcagcagcc ggtcttcctc agcctcaagg tagatgggac
     1381 ggagctcgtt gaggataagt gcaccgtaca tcgagcggtg cgagttccgc tggacgatga
     1441 cgcggtcatt tggatcacac gtcgacagga tcattgcctg gttcccaaca gtcgaaccat
     1501 tgacgaggaa gaaggattcg atggcaccgt atgcttcagc cgcgagttcc tgtgcctcct
     1561 tgactacgcc gatcggacga tggagatagt cgagcccttc aacctcggtc aggtccgaaa
     1621 gaaatacatt ctcgccccag aactccttga gacgtggatg aatcccctgg ccgcccatat
     1681 gtcccggcat gtgaagaccg gcacggtgct tggcgatata tgactggaca gcatcgaaga
     1741 aaggcgtgcg gttttggtct ctcatggaga agagtgtacc ggtcgaaccc ctgatgtcaa
     1801 cgtgcgacga tgaatccggg ttcccgggcc ggcgagctca gaacgtctgt ccaggcgcca
     1861 ggaccatgca gcgggcgctt gtcgacgact ccaccttcgt gcggaacacc tgggggtcga
     1921 cgtcgatcag ggggaacgtc ccgtagtgca ttgggatcac ggtgcgtggc gcaagcatct
     1981 tgacagcctc cacggcatcg tcgataccca tcgtgaagtt gccgccaatg ggcagaagcg
     2041 cgacatccag cgggtgcaga cgtccgatca gctccatatc aagaaacacg ccagtgtcgc
     2101 ctgcgtggta gatggctctc tctcctgtct tgatgacgaa gccgcacggg tttcccgtat
     2161 agacgagatg cccgttctcg gtgaccgacg aaccatgcag ggcctgagta aggatgagct
     2221 caccaaatgg gaaggtgtga cttccaccga tgtgcatggg atgcaccttc gagacgcctt
     2281 tccctgcgac gtacgtgatg agctcagccg gaccgacgac ggtcgctcca gtgcgcttgg
     2341 cgatagggat cgtgtcgcca agatggtcgg catgaccatg cgtaacgagg atgtagtccg
     2401 gatcgacgtc gtcgggatcg actgccgcca aggggttgcc ggagaggaac gggtcgatga
     2461 ggatctcacc ggccggcgtc gtgatgagaa aagccgaatg gccaaggtag gtaagctgtt
     2521 catgtaacat cgtgtcatgt acctccaatc cagtctatcg acagtcgaat acgggtcaag
     2581 gaccgtgcga gatccacctg agacttgcaa ttggccgaga cgtggtacaa tgtgccaagt
     2641 cattcgtttt gcgacagttc gcgcgctgcc aacacccgca ccggggaatt gcagtggcgc
     2701 cggaggaaga acacgtgatc aacaaggtaa cagtcagtgt ccccgcgagc actgccaatc
     2761 tcggtccggg gttcgacgca ttcgggctgg ctctcgaaat gcgcagcacc tataccgccg
     2821 agatcctctc ttcagacgag aagtggagcg tccacttcga cggcatgggt gccgacgtcc
     2881 tcaacgagaa gggttccaag ttcgtcagcg aagtcatgga ctacttcgaa cgggccgaat
     2941 cggtgcatct gccgccaatc agcatcagcg ttcacacggg cattcctctg acgcggggcc
     3001 tgggatccag cgccagtgcg atcgtaggac tcctgtcgtg cttcgaggtc cttatgaaca
     3061 cgaagttcga taccggcaag gtactcagat acgcaattgc catcgaggga catcccgaca
     3121 acgtcactgc atcactggta ggtgggttca cgatcgctgc aagcgacaag agcgaggtcg
     3181 cctacatgaa ggtcgtgccc aaggacctca aggtcatcgt cacggtccca tccagtttcc
     3241 tgtcgacaaa gaaggcccgc gctgtccttc ctgatgtcta cgcgcgcaaa gatgcggtct
     3301 tcaatatcgg tcgtgcctcg atgttgtccg cctgcatctt cagcggcaac tacgacctcc
     3361 tgtcgtttgc gctccacgac cgtcttcacg agcactaccg cgagaagctc gtcaccggat
     3421 ttcgcgaagt atcgcatgcc gctgaccggg cgggagccta cggcagcttt ctctcaggag
     3481 ccggccccag tatcgccgct ctcgccgagc ccgccaaggc ggagaaggtc gccaaggcga
     3541 tgcagaaggc gatgatcgag ctggacgcgg aagcggagac attcgttctc gacattgctg
     3601 cccagggagt catcctgaag accattgacg acgaggaagc atagtccggc tttcctcctg
     3661 gaatcaccga ccgcccgtcg atttcgacgg gcggttcttc ttctcgctca accagtgcgg
     3721 gcgaccagca aagtggcttc agaagccgtg ttcaggactg gcgttgcgaa aagcggccct
     3781 gacagacgat ttcagacgat atgtcgtatc ccagcacgtt ttagttctgt tcagccccgc
     3841 catgagcatg tttcgctgga acgagcctgg gatgcggagt tcagaatgac acccaagaag
     3901 ccgacccctg acagagttat ccacagctcc tgacaggttt tcccccggac ggtcctatct
     3961 gactccacca gttctccaca taactaacag cggttgtgga gaacggtcag cccgggtgac
     4021 atgcaaagac gccccggtga agtgtcgggg cgtctgatgg agttctgacg gtggaagaga
     4081 ccggctaggc cgacggctct acagcttcgc cagtaatcct gtcttctcgt tgaactggtt
     4141 gatgagtcta tcgaattctg gggcggccgt ccagatcgcg tcccagaact caggctccca
     4201 gagttcgcgc agttcttctg cggtcttgcc ttgctgctcg acccaggtga agtacttgag
     4261 gttgtgcagc tgcttgcggt cgcggtagtt gagctcgcga taccagtcag tgagaatgcc
     4321 ctcgagatac tttgcatagt cgagcgccgc gtccatctca gtgtaggggc cgtactcagc
     4381 acgcagttcc tcgagacgcg acaggtacag gtcggccgaa tccgtgaaca cggtgaagac
     4441 aatgtcgttc tcgtccattt cctcgtactt ggccatcttg atcgctgcga ccatgttcgc
     4501 cagagatgag atgcccatga ggttgagctg ctcgatgatc gcgcgcggga cacccttctt
     4561 ctccaggtag gccttgcctg cttcctcgtt gaagaggcgc agcagatgca tcgggtagtc
     4621 gtcgtcgatg gcggcgactg cgtccgtatt gcgaacgttg tggatccagg ggacgtgctt
     4681 gtcgccgata ccctcgatac ggtgggcgcc aaacccgttg gcgtagaggg ttgggcattg
     4741 cagtgcttcg acagcggtga tatggctgta cggatagacc ttcttgaggt actcgccggc
     4801 gctgatggta cctgcagaac ctgtggcaga gacgtagccg gccatacggg acctcggtcc
     4861 cttgatgctg ttgaagacct gctcaaatgc ggagcctgtc atgtcgtagt gccacagcgc
     4921 attgccgaac tcctcgaact gattgaacac gacgatggcg tcaccgcggg tggtcttgag
     4981 ctcgtgcacc ttgtcgtaga tctctttgac gttggactcg gttcccgggg tgccgatgac
     5041 ttccttggtc ccgatctcgc gcagccagtc gaagcgctcc ttgctcatgc cttccggcag
     5101 gatggcgacg gcatccgtgg ccagcagggc gcagtcgaaa gcgccgccac ggcagtagtt
     5161 gccggttgac ggccacacgg ctgcctgaca ggtcgggtcg aactgaccgg tcacgaggcg
     5221 cgggacgagg cagccgaatg cagcgccgac tttgtgtgcc ccggtaggga agtacttgcc
     5281 cacgatgccc acgacgcgag ccttgatgcc cgtgatcgaa ctcgggagct caatatagtt
     5341 cacgtcgccg tacaagccgg tcttcacgtc gttgtgccac gtgatgcgga acaggttgat
     5401 gggattgacg tcccacaggc cgacgccctt gagtttcgcc ttgaccttgt ccgggacaag
     5461 cgacgggtcc ttctgctgtg caaaggtggg gatgatgata tttcggtcct gtgccagctt
     5521 gacggtcgtc gccaggacgt cttcgtggac ggtcatatca aatacagcca tgtctactcc
     5581 tcttgtcaaa gaatccacgc gtcaggcgtg gaaacacaca taataaccag cttgtcagtc
     5641 tagcatgccc gaaggcttgt tcaaccctcg tgtatgaaga gcttgacaag tcgcccggtc
     5701 acatcgcgca gggcggcacc cagcaactct gggtccagtg tcttctccgg ttcgttgtga
     5761 aacacggcga atgggacctg gtgtcccctg actgtaatga acccctcgtc atggcccccc
     5821 gacaggcatg cccagtcgtt gtccttgtaa taccgctcca tcttggcatc ctcgagcgag
     5881 tgatacagga tggagtctgc ctggtcgggg cgatagcgcc tgcggttctt gcgcagcgac
     5941 tcctcgaagg agacgtcgat gtacaggatg acggcgtgcg cgagaacggt gtccgacagg
     6001 tgctggaaag cgttctcaaa gccatgttca ccgccgcggg cgaactccat catgaccgtg
     6061 tggcgctcga agagcagcgg gtcccgcgcc agcttcttgc ggagatccag gtcgatgcgt
     6121 tcgatgaaga agtcccagat gcggtcatcc aggaagtaga gagccttgtc cgtgtcccgt
     6181 ctgccgagcc ccatggactc cctgatcgca tcattctcga aggtctgcca gacgtagaca
     6241 aagtcgtcga gctcctcgaa tggggcgatg tggtacgcct gcaatcgctt ctcgtcgggc
     6301 agctgcttca ggaaatcgat cgtctcactc ttgcctgccg ccggcctgcc gatcaacagg
     6361 atgacatcca gcttgtccat gatacctccg tgtggggttc tgcttccatt gtcttaccgt
     6421 ccacgtgcgg tctccgtcta ggagtgccga gagaggacag atcacagatg cgccaatcac
     6481 cgatgaacag acaccgacag ttgttctgcc aggcaactag cgcttctgct gcagttcctc
     6541 gatgagagcg gtcagccgcg gctcctctcc cggaattcca tctgcgagcc gcgcgacggc
     6601 taccggcagc agccagctcc acatggcttc tcctgcgtcg ggctgcagtt cgcggtagcg
     6661 cttcagccat gccctgtaga accggttcct ggtctcgcgc tccacccacg ctacgcggaa
     6721 ggacccgggc ggggcatcgg cgatcctgag caggagtgcc gtgcgtgcag catcagacgc
     6781 tggtgtccca cgcgcggcaa aggaccagtc gatcaccgaa ggaccctgcg gtgtcatgag
     6841 cacattgtcc gggtgaaagt cgcagtggca aaggctgtct ccgtctggaa gccgctccag
     6901 ggttgccagg gcatcgccct tgacacgcgc gaccacgccc gcacgtgcga tgcgtcgggc
     6961 caggtcctca cgacgggagg tcagtcccgg tgcatgacag tcgttcatgt gtgcctgcag
     7021 ttcggcgaag atgccggcgt agtgagttac ccgcaggggt tcgcggccga gaagagtgag
     7081 catgctggtc ccgctcagac gttccagcac cagtcctcgc cgaccgtcca ccgtgatgac
     7141 ccccctgagc tggggagagg gaatgcctgt gctgttggcc gcccttgtcc catcgacttc
     7201 ctggtcgatc agcgaagaag gaaatccggg gacgaacagc ttgaggaccg agtcttcctt
     7261 ccaggcatac acctccgcgg tccgtccatg accaagcagt gttgccgagc cgatgtgttc
     7321 ttccatagtc tcctcctggc aatctctggc aggcatgtgg ttcatcccat ggacctgaga
     7381 cgtgggacaa gcatccagat gagtattgca ccggtcaatg cgccgcaagc gctcacgata
     7441 acggctacgg gctcgttcgt ccactgcgcc agccagccga caagaagcga gccgatgggc
     7501 atcatgccga agaacaccag gctgtagaca gagctgacgc gccctcgcag tgagtcttcg
     7561 acctgtgact gtatcagtac gttgcagaga ttgacgacca ggatgctggc gaatccgacc
     7621 agacccatca gaacgagtgc gaggcctgtc gagcgggcga tcgcgaacac caggaggacg
     7681 atgggatagg caaagctccc gaacgtggcc agtcgcccct tcactctgag atggctcacc
     7741 gttgcgatgg tcaaggcggc cagcagggca ccgatgccac gcgcggactg gaggaagcca
     7801 ttgatcgtcg ctcccgtcgt cgaagtcccg tgcaggacct tgaccgccca tgccgggatg
     7861 agcgcggcaa acgagaggcc aaacagtgac gtgacgccga tgaggaggat gagcgatcga
     7921 atgtcctcat gtctccagac gtatctgagc ccctcgacga tccctgcagt cgaaccctgg
     7981 ctccgagggc acagaggaac aggtcgaaga cgcatgagca gaagtgcggc gatgactgcg
     8041 acgaaggaca ggccattgat cgtgaagcac catgccggcc ccaggagggc gtacaccgca
     8101 cccgcggcgg caggccccag cgcggtcgcc aggttgaaca tcgatccgtt gagcgcaacg
     8161 gcattgggca ggtgccgcct gtcgtcgacc agctggttca cgagagccag ccgggctggc
     8221 gagtcgaagg tgttggcgat gccgagcaga aaggacagga ccaggatgtg ccatgggcgt
     8281 acggcgcccg tgaatgtgag cactgccaga atgaaggcga gcaccatcat gacagactgc
     8341 gtgtagagca tcagcgtctt gcgcggcacg cggtccgcaa ccgaacccgc atacagcatg
     8401 aacagccaga cggggaggcc gttggcgaat gcaacgtagc cgagaaaggc cgcggacctg
     8461 gtcagctggt aggcaaggta tccctgcgcc gtcatctgca tccaggtgcc gaagagcgaa
     8521 acgagctgac cggcgaacca gatgcggtag ttcgggtatc gcatggctgc gaacgtgttg
     8581 ttcaggctga accgtcgttc cgaaatcggg tcctgcccat caagacactc cacggcagaa
     8641 ctctcgttca acttctggtc aaccattcgt ccgacctcgc ctgtcaggga tcgtgctcac
     8701 cgcacggaac accatgagag tctagcacaa acatcggagg gatggtcgca cgctggattg
     8761 acgtgcccgt tttcgctata ctgcccagaa cagagaggtg cggtgggatc gcgtcgccag
     8821 cgaacgacca tgtcaggaga ggaggatcgg atgaagaaga cgattgcagt gttgcttgca
     8881 ctgagtctag cgttcttcgc aggatgtgga aacccgacgc cgaaagtgga gacggggaag
     8941 gtcaccatca cggtcacgga cggcaatggg gcccccatca agggagcgac tgtcacgctg
     9001 acggacggcg acggcaagga gacgacggct gcgtcgagca gcagcggagt agcagtattc
     9061 gacgcactgc cctatggtga gtatgacgtc gcatcatcca tggccgggtt tgccgatggt
     9121 actgatacga tcaccgtcga ctcgagcagt caggacgtcg cccttgacat gacggcggac
     9181 acgggggact cgacggattc cggtcaggat acggatgcga tcggtgatgt ctccgtcatc
     9241 gattcgctca caagctaccg ctacaagtgg acgacgctct cggagggaga tgcgaggggc
     9301 tcggtcatcg agggtggcac ggagaaaccc aacagtgagt actccattgt gcgtgacaag
     9361 gaagggaagg cccagatgga gttctacaaa gttggcgata ctctcaggat gggtaccgca
     9421 ggcaactggc agacactctc cggtgatgac gccaaggact tcggctacgg cgagtccttc
     9481 atcagcactc tctccaatga ctatgcggat atgaagagca actggtcaga ctggaagaag
     9541 tctgacggag gcgctatcaa cgggtacgac accgacaagt acacgtacag cgggaacgcc
     9601 aacgggacaa ccgtcaccga tacagcctac tacatcaaga gtggtgaatt caagggagtc
     9661 atgacccgct acgacatctc ctaccagagt gacgcggacg cgaccaagcg aagcggctac
     9721 accatcgacg tttatgacct caacaagccg ctcggcatca aactgccctg aggagtgacg
     9781 acgcaggatc gcgcaggcga gggtttgcgc acgctggaaa tgaaaagcgg ggggcttgcg
     9841 ccccccgcgg ttgtcgtacg gtggttggag aaagaaagtc gagaccttct catctaacac
     9901 gtcgcatatc ttagcgtgtt caacggactg cgcaagagtc tgtcaaatcg atgatccttc
     9961 acaatcagaa ggagccctga acgccaactc cgcccgattc tggatagtcc gacgtggaat
    10021 gtcgttctcg tgcgggtctc tgctatactg atcttcgcat caccagtctt tgggcgaaaa
    10081 gggagcctca tggaacgcaa agggtttgtt ctgaaaggca caagtcagtc agtggagatg
    10141 atcatcggcc aggtgacacc gatcggagac atcctccagc agctggacga ggtcctcgca
    10201 aggcggggcg acatcatggg acggctgccg tttgccctgg acacggggga ccgccaactg
    10261 agcaatgccg aactggccgc atttgagcgc ttcttcatcg accatcacct ggactaccag
    10321 ttgccgcaac cggagcccgc cgtcgatcca agcgaagtcc tgttccctgc gctgccatcc
    10381 gacccgacta tcgtcgtcga ccacacactg cggtccggtc agtctgtgac gactggtggc
    10441 aacgtgctgg tcgtcggaga cgtgaatgag ggagccgagg tgcacgcagc gggcaacatc
    10501 tacgtcctgg gtgtgattcg cggggtggcc tcggctggtg agagcatcac ggcgcttggc
    10561 ttcgagccct cgcgcatgac cgtgagcgac gtggaatacc aggcctctga gcgcggcttc
    10621 agggatgcac gcaaggcacg gaccgccttc gtcgagaacg gcgagatcca catcgaagtc
    10681 gcaggaaaaa ggagatagcg ctatgggaaa agtcatcgtc gttacgtcag gcaagggtgg
    10741 agtcggcaag accacgacca cggccaatgt cggtggagca ctgacgaaac tgggaaagag
    10801 ggtactgctc gtcgatatgg atgtgggact gcgcaatctg gacctggtta tgggccttga
    10861 gaaccgcgtg gtgtacacat tcatggacgt actcgaaggc aggtgcacgg ccgatcaggc
    10921 catggtcaag gagaagcacg caggcgggtc gctgatgctt ctggcggcgt ctcagctcca
    10981 caacaagcag gatctggacc tggagaaggt caagaacgca atcgccggcc tggctgcaga
    11041 gttcgactat gtctttcttg acagccccgc gggcatcgaa catggattcg acgccagcag
    11101 tgcacctgcc ggacaggccc ttgtcgtcgt tacccccgat gtcacctcgg tgcgcgacgc
    11161 cgaccgcgtc atcggcctcc tggagaacaa gggggtgcgc gactgcggcc tcatcgtcaa
    11221 caagtacgac cggaccctta tcgagcgcaa gaagcagctg accagcgacg acatcgtgga
    11281 cgtcctcgga gccgagctca caggaatcgt tccggaggac aaggccatca tcgacgccac
    11341 caatcgcggc gacattttgg tgctgagcca gagcacgcct gcggcaacgg cgtacctgga
    11401 gatcgcccac cgactcgagg gcgacgaaac gcacttctcg gcgctgtcga caggacgaag
    11461 ccttctcagc atcttccgga ggcagaagtg agctggttcc acaaagcgac gaacgagatt
    11521 gccaaggagc ggctcacgtt catcctggtg caggaccgtt ctcagctgtc accagcagtc
    11581 atggagcaga tgaaggagga actggtcgcc gtcatcggca agtatttcga catcgacaag
    11641 gctcagatcg agctcaacgt tgagcgtcag gaaggaaggc aggtcgtcga gacatctatt
    11701 cccatcaacg gcctccggca ccagtagcgc ccgggcggac gccggcgcac tggcgacgac
    11761 tctccgcgtg gatacgaccc ggcaggtggt cacgcgtagg gaattgtgta caatgaaatc
    11821 ccgattccgt acggtcggcg cgggcagacg atcactatgg cacgagagga cgaacatggc
    11881 gtcaggagca agcgcacagc ctcaaggtac tctgacactg agccccgaac aagtccacga
    11941 actgctgccc cagagggagc cgtttctctt cgtcaacggt cccgcggaag tcgttcccgg
    12001 cgttcgcgtc tcagcgcggg caacctatgc gcaggacagc tccttctatg ccgggcactt
    12061 ccccggattt cccctgacgc ccggcgtcat catcatcgag accatggcgc aggctgcctc
    12121 cctgatgatg ctgaccatgc ccggctgctc gggtcacatc gtctacttcg tgggcatcag
    12181 ggaggcgagg ttcttcaggg cggtccttcc cggagcggtc gtagagatca tggggtctgt
    12241 cgtttcgaag cgtcacggca tcgtcgagag cgggatggaa gcgtggacga atggtctgcg
    12301 cgctgcttct gcgtttgtga cgtgtacgct gcgtcctcgt cgaggctctc tggacgtgaa
    12361 ggagccgtcc gatgaatgaa gtgggttggg ttgtggaaca gtcttgttgc agggaacgca
    12421 agggcagcgt cacgaacagg cgggagagtt gagatggtag cacagcatac cggcaagaat
    12481 agcatgagga tcatcatgac ggttgcggat cggatgtgga gagggttcct catcaactgg
    12541 ctggctgcca gcccgcttct cgtgaatcag acgagagcgt cactgatgcg cctctacgga
    12601 attcggacag caacgcacgg gattcgtccc ggatgctact ttgccggacg ggatgtcgtc
    12661 atcggcgctg acacgttcgt gaactaccgc tgtttcttcg acgggtcggc gcccatccgt
    12721 atcgggagga agtgcgggat cggcatggaa gtgctgttct gcacgtcgac acatttcctg
    12781 gggccgacga ccgagagggc aggacccgtc aatccccagc cgatcacggt tggagacggc
    12841 tgctggatcg gtgcccgctc ggttatcctg ccgggcgtga ccatcggcga tgggtgtgtc
    12901 atagctgcag gagctgtcgt caacaaggac tgtgccccca acggcatgta cgcaggcgtt
    12961 cccgcccggc gtaccaggga gctgccagtc gaggattgaa gccacaggac ggacgtcgcg
    13021 catgtgatcg gtcgctcagg gggcgtatgg tggaacagct ggacaaggga taccttttca
    13081 cggacatcgg cgcgcttgag gtggccggaa acgaagatgg catagtgtct gtgacgtttg
    13141 tcgagaacgt cgacacggcg gcggcatgtg tgacgt
//
LOCUS       DATMZD010000182         2841 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_136515,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000182 DATMZD010000000
VERSION     DATMZD010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136515"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1526)
                     /locus_tag="VN478_03745"
     CDS             complement(<1..1526)
                     /locus_tag="VN478_03745"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWQ21820.1"
                     /translation="MLNARKLLAAVMALLVAFTSVLAAVPSAKAESLTTIEVYIGKNT
                     GTVNGKSTTLEQGAVISNGRTLVPLRFITEAMGAALGWEAATRTANIALGDNKIALTI
                     GKTVAKVNGYEVALDAPAAIINGKTVVPLRFVAESMGATTAWNATLKKVTVQFSMDWL
                     KNKAVVPFWEAMAAKLGESLTALTKEFNATHPSMEVQLVQMANYNTLQTKTIGAVAAK
                     DPPLIAQAYENWAAQFATGFYLSTFDSYINGSNGLSKAEIADFFPKMWDSSKLADGKR
                     YMMPFNKSSVVMYYNKDMFKAAGITRAPTTWQEFADDCVKLTKTDDKGEQTQWGASHT
                     PSVDLWYGLVYAYGGRVLNDTYDKVLFANNNGVKAGTQLFADLYAKKYMHYTTAYGDQ
                     TDLGVGKAAMTFGSVAGRTYYEQAVGGKFELGEAPLPAGPAGEAAALYGTNIVMFGNA
                     PKYTQRQKDAAWAYIKWFTSPHTQAVWAAQTGYMPARQSSLKDPVLVAAYAAKPETRA
                     G"
     gene            complement(1576..2421)
                     /locus_tag="VN478_03750"
     CDS             complement(1576..2421)
                     /locus_tag="VN478_03750"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HWQ21821.1"
                     /translation="MTTMRQSVLWNKINRILLYVAIAVLFILVVFPFYWMIVTSLQPQ
                     DAVFSVPPQLWPKHLTFQNYIDAWNAAPWLRYFGNTLFVAVASTLISLATSSLAAFAF
                     ACIDFKGKEALFSLVLSVMMIPAATTLIPNFLIIRTLGWYDKFYALIIPFAASVFGIF
                     LLRQYFMSMPKELWEAAQIDGASRLRYFRSIALPLAKPAIVTIAINSFLGSWTSFLWP
                     TVMTQSQNKQLIEVALNSFLGEKGQQWSQLAAASVFTTIPIVVLFFFVQRQFIEGIGG
                     GGIKG"
     gene            complement(2432..>2841)
                     /locus_tag="VN478_03755"
     CDS             complement(2432..>2841)
                     /locus_tag="VN478_03755"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HWQ21822.1"
                     /translation="AMILYSTWHDIGFAVIIFMAALSKVPTELLEAAQIDGASGWRTF
                     WHVTWPMISPSTYFVVIIYTISAFKMFTQVAVLTQGGPVDATTTTGYYLYQQAFGQMH
                     FGYASAVSIGLFVIIFLLTMLQRKLTGHKVFYG"
BASE COUNT          592 a          796 c          859 g          594 t
ORIGIN      
        1 ccggctctgg tctcgggctt ggctgcgtac gccgcgacta ggaccggatc cttgagggaa
       61 ctctggcgcg ccggcatgta gccggtctgt gctgcccaga cagcctgcgt atgaggtgaa
      121 gtgaaccact tgatatacgc ccaggccgcg tccttctggc gctgcgtgta cttgggggcg
      181 ttgccgaaca tgacaatgtt ggtgccatag agcgctgcag cctcgcctgc aggtcccgcg
      241 ggcaggggcg cttcgcccaa ttcgaacttg ccgccgactg cctgttcgta gtaggtgcga
      301 cctgccacgg agccaaacgt catggcagcc ttgcccacgc ccaggtcagt ctggtcgccg
      361 taggccgtgg tgtagtgcat gtacttcttg gcatacaggt ccgcaaagag ctgcgtcccc
      421 gccttgacgc cattgttgtt ggcaaacagg accttgtcgt aggtgtcatt caggactcgg
      481 ccgccgtagg catagacgag cccataccac agatcgacgg acggcgtgtg cgaagcgccc
      541 cactgagtct gctcaccctt gtcgtcggtc ttggtcagct tgacacagtc atcagcgaat
      601 tcctgccatg tggtcggcgc tcgcgtgatg cctgcagcct tgaacatgtc cttgttgtag
      661 tacatgacga cgctggactt gttgaacggc atcatgtagc gcttgccgtc tgcgagtttc
      721 gacgaatccc acatcttggg gaagaagtcg gcgatctcgg ccttcgacag accattggaa
      781 ccattgatat agctgtcgaa cgtgctgaga tagaacccgg tagcgaactg ggctgcccag
      841 ttctcatacg cctgcgcgat gaggggtggg tcctttgccg ctacggcacc gatggtcttg
      901 gtctgcagcg tgttgtagtt ggccatctgg accagctgga cctccatgct ggggtgggtg
      961 gcgttgaact ccttggtgag agcagtcaga gactcaccga gttttgccgc catggcttcc
     1021 cagaacggga cgacagcctt gttcttcagc caatccatgg agaactggac agttaccttc
     1081 ttgagtgtcg cgttccatgc ggtcgtcgcg cccatcgact cggcgacgaa acgcagggga
     1141 accacggtct tgccgttgat gatagcggca ggagcatcca gcgcaacctc gtagccattg
     1201 actttcgcga cggtcttgcc gatcgtcagt gcgatcttgt tgtcgcccag cgcgatgttg
     1261 gcggtcctgg tcgcggcctc ccatccgagc gcagctccca tcgcctcagt gatgaagcgg
     1321 aggggaacca gcgtgcggcc gttcgagatc accgcgccct gttcgagcgt cgtactcttt
     1381 ccgttgacag tgcccgtgtt cttgccgatg tagacctcga tcgtcgtcag cgactctgcc
     1441 ttggcactcg gaacagcagc gagtaccgag gtgaatgcca cgagcaacgc cattacagca
     1501 gccagcagct tacgtgcgtt gagcatactt cctcccctgg agaattggtg gtaacattca
     1561 actacatcag aacggctacc ccttgatgcc gcccccgccg ataccttcga tgaattgcct
     1621 ctgcacaaag aagaacagga cgacgatggg gatcgtcgtg aaaaccgatg ctgcggcaag
     1681 ctgagaccac tgctgtccct tctctcccag gaaggagttc agggcaacct cgatcagctg
     1741 cttgttctgc gactgggtca tgaccgtggg ccacaggaaa gacgtccacg aacccaggaa
     1801 cgagttgatg gcaatggtga caatcgcggg cttcgcgagt ggcaaggcaa tgctccggaa
     1861 gtagcggagg cgcgatgcgc cgtcgatctg tgctgcctcc cagagctcct tgggcatcga
     1921 catgaagtac tgccgcagca ggaaaatgcc gaagacgctt gctgcaaacg ggatgataag
     1981 ggcatagaac ttgtcgtacc agccgagcgt ccggatgata aggaaattcg ggatcagtgt
     2041 cgtagctgcc gggatcatca tgacgctgag gaccaggctg aacagagctt ctttcccttt
     2101 gaagtcgatg caggcgaagg cgaaagcggc cagagacgat gtcgccaggg aaatcagcgt
     2161 cgaggccact gccacaaaca gcgtattgcc gaaatatcgc agccatggtg cagcattcca
     2221 ggcgtcaatg tagttctgga acgtcaggtg tttcggccat agttgaggcg gcacgctgaa
     2281 gacagcgtcc tgaggctgca aagacgtcac gatcatccag tagaacggga atacgaccag
     2341 gatgaacagt actgcaatcg ccacatagag gagaatcctg ttgatcttgt tccagagaac
     2401 agactgtcgc atcgtcgtca tgtcacacct gtcatccgta gaagaccttg tgtcctgtga
     2461 gcttgcgctg gagcatcgtg agcaggaaga tgatgacgaa caggccgatg gacacggcac
     2521 tggcatatcc gaaatgcatt tgcccgaatg cctgctggta caggtagtat ccagtcgtcg
     2581 tcgtggcatc gacaggaccc ccctgcgtga gcacggcgac ctgcgtgaac atcttgaagg
     2641 cactgatcgt gtagatgatc acgacgaaat aggtggaggg agagatcatg ggccacgtga
     2701 catgccagaa ggtcctccat ccgctggctc cgtcgatctg ggcagcctcg agcagctcgg
     2761 tcggcacctt ggacagcgcg gccatgaaga tgatgacggc gaatccgatg tcgtgccacg
     2821 ttgagtagag gatcattgca g
//
LOCUS       DATMZD010000183         8751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_36068,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000183 DATMZD010000000
VERSION     DATMZD010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8751
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36068"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..1040
                     /locus_tag="VN478_03760"
     CDS             90..1040
                     /locus_tag="VN478_03760"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWQ21823.1"
                     /translation="MTPSSTRRLAADGAITALVLALPLVFRTKPSIIGYLNIAMLYAI
                     ATQGLNLLMGYGGLVSLGHAAFMAIGGYTAAVLVMTYHVNMFLAIVAGVAGAGLFGLI
                     IGFPSLRLSGFYLAVATMALGSSVADVIKRLPITGGDYGLINIPVLRLGGWTLTSEAS
                     KYYFFVVILVGVFLIVRNFLSSRSGRAVRAVRDSEMTASAMGVNVAAYKLVTFIFAAC
                     IAGLSGALYAFTISYLHPMNFGLAFSINLLSMSIIGGLGTIIGPILGALFWVMLPVVI
                     GGRMEFLSTVVFGLSIIGSVMFLPKGLSELVTRIQTRLKL"
     gene            1088..2278
                     /locus_tag="VN478_03765"
     CDS             1088..2278
                     /locus_tag="VN478_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006313856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HWQ21824.1"
                     /translation="MSVGILGSYSSRCGKLENSTIYDLIVEAGRGAIADAGIAARDID
                     GIWMGNFSAGSFNNQEHIAAWGVEIDPALLYKPCTRAEVACASGSAAVRGARLAIGAG
                     EARFVLVIGVEKMTTLDRDGVTHALAMASYWPEEGSKGMTFPGLFGMWSKAYREHYGY
                     TDEQMDLWQAMVHTKNRDNATHNELAHMYTKPSSLEFALHVSEKNPLVADPLKLTDCS
                     LVSDGAAAMVLGPIDDVKALKGRAVEITSQSLVTDHMQSSKRELWHNVAGKMAVDSAY
                     SKAGITASDVDVAEVHDCFTINELLSYEAMGLCPEGEGGRLIEHGGVVQPDGSCPINL
                     SGGLIGKGHPVGATGVSMHVYVSRQLEGRPLGLGAKNPEVGIVMNVGGSNVSNFASVL
                     KRVK"
     gene            2293..3078
                     /gene="fabG"
                     /locus_tag="VN478_03770"
     CDS             2293..3078
                     /gene="fabG"
                     /locus_tag="VN478_03770"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HWQ21825.1"
                     /translation="MSQGYGLQDKVVIVTGGAAGIGRATAMRFAEEGARVSIWDMNAE
                     AGAVTLADLKSKNAEAMFQLVNVADAEAVEKAVADVVTAWGHVDVLVNNAGITRDGQL
                     VKYKEGVVTGTMSDASFDAVIGVNLKGVFVSTRAVAPYMIKQGHGVILSASSVVAANG
                     NFGQTNYAATKAGLIAMTRTWSRELGKYGIRVNAVAPGFIMTDMVAAMPEKVLEGMVA
                     HTPMGRIGSPEDVANAYVWLASDQAAFVHGSTLAVDGGIVLGT"
     gene            3143..4441
                     /locus_tag="VN478_03775"
     CDS             3143..4441
                     /locus_tag="VN478_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012003114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HWQ21826.1"
                     /translation="MQQGSWTNKVVSMDEALGRIASGDTVVVGMAAAQPQGFLSSLHT
                     IRDRVHGVKVVSCLLLKEYPFLADVRADADTPFKMESWYFGNSERELYKKGLVSFIPN
                     NLHNAGREKIQAEPINVFVGTATPPDSHGFMSLSLCLVYEKDMIENADLVILEINENL
                     PRTFGDTQVHVDDVDLLVQNNAPLLTIPVVQASDVEQHIGENIAQLIDDGATLQIGIG
                     GIPNAIMKFLSDKKHLGIHTEMITDGIVDLVRAGVIDNTRKTLHKGKIIGAFAMGTEP
                     LYEFINENVGVDILRGSYVNDPYVVAQNDRMVSINTSLQVDLTGQVCSESIGHRHYTG
                     TGGQLDMHRGATLSRGGKGIIALRSSVKDDEISTIVPMLSPGSFVSIPRQDTDYVVTE
                     FGAAHLKGRSVHDRALALIAIANPKFRESLALEAKKIGVL"
     gene            4608..5480
                     /locus_tag="VN478_03780"
     CDS             4608..5480
                     /locus_tag="VN478_03780"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWQ21827.1"
                     /translation="MKQAAAGRMAGIIFGTLAGAAFADGFLLEPFQLDVKRVEVASPR
                     LPGGFDGLKIAQLSDLHLHRISRAYRTAIGVVGREHPDLIVITGDLVDRSDETAACIS
                     FLSSLERAAGVPVVVVPGNWDHRAFPTRQSIAAWHERLQAQTGIRVLANRNVVLWRHG
                     ERIWLVGTDDPYFGHADLDASFEGIPDSAFALVLTHAPEAFEELSQRPAARLVLAGHT
                     HGGQVRLPFVGAVRVPSRYGTRFAGGLYQLGDTIFYVNAGMGMSHLPVRFLCRPELTF
                     ITLKSCGPGELSSW"
     gene            5474..6319
                     /locus_tag="VN478_03785"
     CDS             5474..6319
                     /locus_tag="VN478_03785"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWQ21828.1"
                     /translation="MVTARRHRRLFWAVSLVVALLLDTVVVEPHVLLVRRIEVAALGL
                     PAEWDGGTVIQLSDPHVGYPSPAAWRAIDVVRGERPHLVVVTGDLADRHSSVPVVLTW
                     AKQLSEATDAPIIYVPGNHEHWRFDDSGDMAQFLDQLRHAGFVVLENELTRIGRGSSG
                     VGLAVVGLDDSYSRHMDVEAGFSSLAADEHAIVLEHCPDDVVSVVAAGHASLVLSGHT
                     HGGQVRLPVWGDRLTAALEGSPFVRGSYRVEGVPLYVSQGLGMSVLPVRFFCPPEVTV
                     FTLRM"
     gene            complement(6428..>8751)
                     /locus_tag="VN478_03790"
     CDS             complement(6428..>8751)
                     /locus_tag="VN478_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HWQ21829.1"
                     /translation="PVGNSGGYSDRDSSRSPNQSGGSNRGFGGFPSGGGGSAIPSGGC
                     LRYLIIFAVIAVAVYLLYNLFSGGLGQLTNPGSTGPSGFQSPTSTSVETYVDPGAHPV
                     STSVSPLARDRFTTLRGNGVDTTTVMVYMCGTDLESESGMATADLQEMLDAEISDKVN
                     IVVETGGASRWQNNIVSNQTNQIYRVTGQGLQLLVKDLGRKSMVSSSTLSDFIQYCKA
                     HYPADRYMLVMWDHGGGSLSGYGYDQYFPNTSMTLDKIGTALKNGGCRFDVVGFDACL
                     MATLEDAIVLEPYADYMIASEEAEPGTGWYYTGWLTALSRNTSIATTDLSKVLIDDYI
                     SESMAQTSENQATLSLVDLAELKGTVPSSFTAFSSSTTKLIDTDKYQVVSNARAGAKE
                     FAASAGINQIDLINFAENLGTGEAKSFADVLRGCIKYNRSSSNITNANGISIFFPYNK
                     LSELTPILQAYDEAGIDSEYSKCLKSFASVAAGGQVVSTGSGNLLDTLLGGMSSGTQT
                     PPSTGSSGVDVVQQLLDAFLSGGDYSSITGGKAGTTGWLDVNRMKSSVQYYGDNQIDP
                     SALVVTEKNGQRVLALSEEQWKLVQDLEQNVFIDDGKGFIDLGLDNVYDFNDGGDLIM
                     EYDGTWLALNGHVVSYYMTSDDRDGDTYTIRGRIPAMLNGQMVDIIVSFSNEFPEGVV
                     LGAQVKYDLRKQTATVPRGLLTIAKGDKIDYLCDYYTYAGKYSDTYFLGRQYTATGTW
                     KIENLSVGNGKYQMTYRLTDIYNNQYWTPSIAD"
BASE COUNT         1704 a         2515 c         2731 g         1801 t
ORIGIN      
        1 aaggacagct tcatgctcat cgtcatcgtc attgtcttgc tggtcttgcc gcaggggctg
       61 tttggcaagc gccagcagag gaggttctag tgactccctc cagtacaagg cgattggcag
      121 ccgacggagc catcacagca cttgttctgg ccctccctct ggtgttcaga acgaagccgt
      181 cgattatcgg ctacctcaat atcgccatgc tgtatgccat cgctacgcag ggactcaacc
      241 tgctcatggg ctatggtggt ctggtttctc tggggcatgc cgcattcatg gctatcggtg
      301 gctacacagc agcagtgctg gtcatgacct accacgtgaa catgttcctg gctatcgttg
      361 ccggcgtggc tggtgccggg ttgtttgggc ttatcatcgg gttcccatca ctgcgtctgt
      421 ctggattcta cctggcggtc gcgacgatgg ctctgggaag ttctgtcgca gacgtcatca
      481 agaggctccc cattaccggg ggagactatg gtctcatcaa cattccagtt ttgcggctcg
      541 gaggctggac actgaccagc gaggcgtcca agtactactt ctttgtcgtc attcttgtcg
      601 gcgtgttcct gatcgtccgc aatttcctga gcagccgcag cggacgggcg gtgcgcgctg
      661 tgcgtgactc tgagatgacg gcttcggcga tgggtgtgaa tgttgcagcc tacaagctcg
      721 tcacgttcat attcgcggcc tgtattgccg gtctctccgg ggcgctgtac gccttcacga
      781 tctcatacct ccaccccatg aacttcgggc tcgccttctc catcaatctc ttgtccatga
      841 gcatcatcgg cggcctgggc acgatcatcg gtcctatcct gggcgcgttg ttctgggtca
      901 tgctgcccgt cgtcatcggt ggcaggatgg agttcctgtc aacagtcgtc tttggtctca
      961 gcatcatcgg ttcggtcatg ttcctgccca aagggctgtc cgagctggtc acgcgcatac
     1021 agactcgctt gaaactctga cgcagcaggc gtacggcgaa acacaactcg caacagagga
     1081 ggacactatg agtgtcggaa ttctgggaag ctacagcagc aggtgtggca agctcgaaaa
     1141 ctccactatc tatgatctca tcgtcgaggc tgggcgcggc gcgatcgcgg atgcaggtat
     1201 tgcagcgaga gacatcgacg ggatctggat ggggaacttc agcgcaggct cgttcaacaa
     1261 ccaggaacac atcgccgcgt ggggtgtgga gatcgacccg gctcttctgt acaagccgtg
     1321 tacgcgggcg gaagttgcgt gtgcctccgg ttcggcggcc gtacgcggtg cgcgcctggc
     1381 catcggtgcg ggggaggccc gcttcgtgct ggtcatcggg gtcgagaaga tgacgacgct
     1441 cgaccgggat ggcgttaccc atgcacttgc catggcatca tactggccgg aagaggggag
     1501 caagggcatg acgttcccgg ggctgttcgg catgtggtcg aaggcgtacc gcgagcacta
     1561 tgggtacacc gacgagcaaa tggacctgtg gcaggccatg gtacacacca agaaccgtga
     1621 caacgcgacc cacaacgagc tcgcgcacat gtacacgaag ccgagtagcc tggagtttgc
     1681 gttgcacgtg tctgagaaga acccgcttgt cgcggatcct ctgaagctca ctgactgctc
     1741 cctggtcagc gacggggctg cggccatggt gctggggccg atcgatgacg tcaaggctct
     1801 caagggacgt gccgtcgaaa tcacgtcaca gtcgcttgtc acggatcata tgcagtccag
     1861 caagcgcgag ctgtggcaca acgtcgccgg gaagatggct gtcgactcag cctacagcaa
     1921 ggccggcatc accgcatcag acgtcgacgt cgcagaggtc cacgactgct ttacgatcaa
     1981 cgagctgctg agctacgagg cgatgggcct gtgtcccgaa ggggaagggg ggcgcctcat
     2041 cgagcatggt ggcgtcgtgc agccggacgg cagttgcccg atcaacctca gcggaggtct
     2101 catcggcaag ggacatcccg tgggagccac cggcgtgtcg atgcacgtct acgtctcccg
     2161 ccagcttgaa ggccgtccgc tcggcctcgg cgcaaagaat ccagaggtgg gtatcgtcat
     2221 gaacgtgggt ggttcgaacg tcagcaactt cgcatcagtg ttgaaacgcg tcaagtagac
     2281 agggaggaaa ccatgtcaca gggatacggt ctgcaggaca aggtcgtcat cgtcacgggc
     2341 ggagctgccg gcatcggaag ggcaaccgcc atgaggtttg ctgaggaggg cgcgcgcgtc
     2401 agtatctggg acatgaacgc cgaagccgga gcagtcacgc tggcagacct gaagtcgaag
     2461 aatgccgaag cgatgttcca gcttgtcaac gtcgccgacg ccgaagccgt cgagaaggca
     2521 gtggcagacg tcgtcacagc ctggggccac gtcgatgtgc tggtcaacaa cgccgggatc
     2581 acgcgcgatg gtcagctggt caagtacaag gaaggagtcg tcacggggac gatgtcggac
     2641 gccagcttcg atgccgtcat cggtgtcaac ctcaagggag tattcgtctc gacgcgcgcc
     2701 gtagctccgt acatgatcaa gcaggggcat ggcgtcatcc tgagcgcgag ctccgtggtt
     2761 gcggcgaatg gcaatttcgg ccagaccaac tacgccgcca ccaaagccgg gctcatcgcc
     2821 atgaccagga cctggtctcg agaactgggc aagtatggaa tacgcgtgaa cgccgttgcc
     2881 ccaggattca tcatgacgga catggtcgct gcgatgcctg agaaggtact ggaagggatg
     2941 gttgcccaca cgcccatggg acgcatcgga tcgcctgagg acgtggcaaa tgcctacgta
     3001 tggcttgcat ccgaccaggc tgcgttcgtt cacggctcca ctctggccgt ggatgggggc
     3061 atcgtcctgg gaacatgagc cagcacgacg gaccacgcct cgtcgagaga cgcggagcac
     3121 actaatgtcg cgggagggaa ccatgcagca gggatcctgg acgaacaagg ttgtctcgat
     3181 ggatgaagcg cttggccgga ttgcgtcagg agacacggtt gtggtaggaa tggcggcggc
     3241 ccagccacag ggcttcctct catcgctcca cactatccgg gatcgtgtgc acggcgtcaa
     3301 agtcgtgtcc tgccttctct tgaaggagta cccgtttctg gccgatgtcc gggccgacgc
     3361 cgatacgccg ttcaagatgg agagctggta ttttggcaac tccgaacgcg agctctacaa
     3421 gaagggattg gtgtcgttca tccccaataa cctgcacaat gccgggcgag agaagatcca
     3481 ggcagagccg atcaacgtgt ttgtcggaac ggcgacgcca cctgacagcc acgggttcat
     3541 gtccctgtcg ctgtgtctcg tctacgagaa ggacatgatc gaaaacgccg acctcgtgat
     3601 actggaaatc aacgagaacc tgcccaggac gtttggagac acgcaggtac acgtcgacga
     3661 cgtcgacctg ctggtgcaga acaatgcgcc gcttctgacg attcccgtcg tccaggcgag
     3721 cgacgtcgag cagcacatcg gcgagaatat cgcgcaactc atcgacgacg gagctaccct
     3781 gcagatcggt atcgggggta tcccgaacgc tatcatgaag ttcctgtcgg acaagaagca
     3841 cctggggatc cacacggaga tgatcactga cggcatcgtc gacctggtca gggcgggagt
     3901 catcgacaac acccggaaga cgcttcacaa aggcaagatc atcggcgcct ttgccatggg
     3961 gacggagccc ctgtacgagt tcatcaacga gaacgtcgga gtggacattc tcagaggttc
     4021 ctatgtgaac gacccgtacg tcgtcgcaca gaacgaccgt atggtaagca tcaacacgtc
     4081 gctgcaggtc gacctgacgg ggcaggtgtg cagcgagtcg attggacatc gacactacac
     4141 cggcaccggc ggtcaactgg acatgcatcg tggcgctacg ctgtcccgcg gggggaaagg
     4201 catcatcgca ctgcggtcgt cggtgaagga tgatgagatc tcaaccatcg tgccgatgct
     4261 cagcccaggg agtttcgtct ccataccgcg ccaggataca gactatgtcg tcacggagtt
     4321 tggcgcggcg cacctgaagg ggaggagcgt gcatgaccgc gcgcttgccc tcatcgcaat
     4381 agcgaatccg aagttcagag agagcctggc actcgaggcg aagaagatcg gcgtgctcta
     4441 gtctacgact cacaacacag gaggcgacat tgtcatcctc cttcctttcc gacgatggcg
     4501 gcgcagcagc gccgccatcg tgcttcctgc cctgcactgt tgctcctttc ctgttgcaca
     4561 gctatactcg caatgtcgcg tttcagccgg ggccattgga gagagatgtg aaacaagcag
     4621 cagcaggcag gatggcaggc atcatattcg gcacactcgc gggtgcagcc ttcgcggatg
     4681 ggttcctcct ggagccgttc cagctggacg tcaagcgggt tgaagtcgcg tctccgcgtt
     4741 tgccgggcgg tttcgacggc ctgaagatcg cgcagttgtc ggacctgcac cttcatcgca
     4801 tctcacgtgc ctatcgcaca gcaatcggcg tcgtcgggag agagcatccc gacttgatcg
     4861 tcataacagg cgacctggtc gatcgatcag acgagacagc tgcctgtatc tcattcctga
     4921 gcagcctgga gagggcggct ggagtaccgg tagtagtcgt acccggcaac tgggatcatc
     4981 gggcctttcc gacaagacag agcattgctg catggcacga gcgcctccag gcgcaaacgg
     5041 gcattcgggt tctggcaaat cggaatgttg tgctgtggcg acacggtgaa cggatctggc
     5101 tggtgggcac ggacgacccg tacttcggtc atgcagatct ggatgccagc ttcgagggca
     5161 tccctgacag tgcattcgcg ctcgtgctga cacacgctcc tgaggcgttc gaggaactgt
     5221 cacagcgtcc tgccgcgcgg ctggtcctgg cggggcacac ccatgggggt caggttcgac
     5281 tgccgttcgt tggggcagtg cgagtcccct cgcgatacgg tacccgcttc gcgggaggcc
     5341 tgtaccagct gggtgacacg atcttctacg tcaatgccgg aatgggtatg agccaccttc
     5401 ccgtgcggtt cctgtgccgg ccagaactga cgttcataac actcaaatca tgcggaccgg
     5461 gtgagttgtc ctcatggtga cagcccgcag gcacaggcgc ctgttctggg cggtatccct
     5521 ggtcgttgct ctcttgctgg acactgtggt cgtcgagccg cacgtgcttc tcgtacgtcg
     5581 tatcgaggta gcggctcttg ggttgccggc cgagtgggac ggagggactg tcatacagtt
     5641 gagtgaccct catgtcggct atccatcccc ggctgcgtgg cgtgccatcg atgtggtccg
     5701 tggcgaacgt ccccaccttg tcgtggtcac tggagacctc gccgacaggc attccagtgt
     5761 ccctgttgtc ctcacatggg cgaagcagct ctctgaggcg acggacgcgc cgatcatcta
     5821 tgtgcccggg aaccatgagc actggcgctt cgacgatagc ggggacatgg cacagttcct
     5881 ggaccagctt cgccacgcgg gcttcgtagt cctggagaat gaactgaccc ggatcggtcg
     5941 cggctccagc ggggtcgggc tggctgttgt gggactggac gactcataca gccgccacat
     6001 ggacgtcgaa gccgggttct cgagtctggc agcggacgag catgcgattg tcctcgaaca
     6061 ctgcccagac gatgtcgtca gcgtcgtggc agcggggcat gcttcgctgg tgctgagcgg
     6121 tcacacgcac gggggccagg tgaggcttcc cgtgtggggt gaccgcctga ccgctgccct
     6181 ggaggggtct ccgttcgtgc gcggctcgta ccgggtcgag ggtgtccctc tctacgtcag
     6241 tcagggtctc ggcatgagcg tcctgcccgt tcggttcttc tgtcctcccg aggtcacggt
     6301 cttcacgctc aggatgtagc aggagagccg ccgcagcgga ccgccgaggc acagtggtac
     6361 cttgatgcgg gacgtggagg tccagcctgt gtgtggtgca gtctccagct cactgccgtg
     6421 acgcgtgcta atcggcgatg gacggcgtcc agtactggtt attgtagatg tcagtcaggc
     6481 ggtacgtcat ctggtacttc ccgttaccga ccgacaggtt ctcgatcttc caggtacccg
     6541 tcgccgtgta ttgcctgccg aggaagtacg tgtcgctgta cttgccggca tacgtgtagt
     6601 agtcgcagag gtagtcgatc ttgtcaccct tggcgatggt caggaggcct ctcggcacgg
     6661 ttgcggtctg cttcctgagg tcgtacttga cctgggcgcc gagcacgacg ccctcaggga
     6721 actcattgct gaacgacacg atgatgtcga ccatctgacc gttgagcatg gcggggatgc
     6781 gtccccggat cgtgtaggtg tctccgtcgc ggtcgtcgct ggtcatgtag tagctgacga
     6841 catgcccatt gagcgccagc cacgtaccgt cgtattccat gatgaggtcg ccaccgtcgt
     6901 tgaagtcata gacgttgtca agccccaagt cgatgaagcc cttgccgtcg tcgatgaaca
     6961 cattctgttc gagatcctga acgagtttcc actgctcctc cgagagtgcc aggacacgtt
     7021 gcccgttctt ctccgtgacg acaagggcgg atgggtcaat ctggttgtcc ccgtagtact
     7081 ggactgagga cttcatgcga ttgacatcga gccagccggt ggtgcccgcc ttgccaccgg
     7141 tgatgctgct gtagtcacca ccggacagga acgcgtcgag gagctgctgc accacatcga
     7201 cgccagatga cccggtcgag gggggggtct gtgtcccact ggacatacca cccagtagtg
     7261 tgtcgagcaa gtttccgctg ccggtcgaga cgacctggcc gcccgcggca acgctcgcaa
     7321 agctcttgag gcacttgctg tactcagagt cgatacctgc ctcatcgtac gcctgcagta
     7381 tcggggtaag ctccgacagc ttgttgtagg ggaagaagat cgagatcccg tttgcgttgg
     7441 tgatattcga cgaagaccga ttgtacttga tgcatccgcg aagcacatca gcaaaggact
     7501 tcgcctcgcc ggtgccgaga ttctccgcga agttgatcaa gtctatctgg ttgatgccgg
     7561 cagacgctgc aaactccttg gcaccggcac gtgcgttgga cacgacctga tacttgtcag
     7621 tgtctatgag cttggtcgtc gatgacgaga acgccgtgaa cgacgacggc actgtcccct
     7681 tgagctcggc aaggtcgaca agtgagagag tggcctggtt ctcagacgtc tgggccatag
     7741 actcactgat gtagtcgtcg atcagcacct tgctgaggtc agttgtcgca atggacgtgt
     7801 tgcgggagag agcagtcagc caaccggtgt agtaccagcc cgtgccaggt tcggcttcct
     7861 ccgacgcgat catgtagtcg gcatagggct ccaggacgat cgcatcctcc agtgtggcca
     7921 tcaggcaggc gtcaaagccc accacgtcga atcgacagcc accattcttg agggcggtgc
     7981 cgatcttgtc cagcgtcatg ctggtattcg ggaagtactg gtcgtagcca tagccactca
     8041 gcgaaccgcc gccgtggtcc cacatgacaa gcatgtagcg atcggctgga tagtgcgcct
     8101 tgcagtattg gatgaagtcc gagagagtcg aggaactgac catcgacttt ctgccaaggt
     8161 ccttcacaag cagttgcagg ccctgccccg tcacacggta gatctgattt gtctggttac
     8221 tgacgatgtt gttctgccac ctcgaggccc caccggtctc aacaacaata ttcaccttgt
     8281 ccgagatctc ggcgtccagc atttcctgca agtctgcagt agccattcca ctctctgatt
     8341 caaggtcggt tccacacatg tagaccatga cagtcgtcgt atcgacgccg tttcccctga
     8401 gagtcgtgaa cctgtcacga gccagtgggg agacagacgt gctgacggga tgtgcccctg
     8461 gatcgacata ggtctcgaca cttgtcgacg tcggtgactg gaagccagag ggtccggtgc
     8521 tgccggggtt cgtcagttga ccgagtccac cgctgaacag gttgtacagg aggtatacgg
     8581 caacggcgat gacagcgaat atgatgaggt atctcaggca gccgccggag ggaatggcgc
     8641 tccctccccc gccggacgga aatcccccga atccacggtt tgaaccgcct gactggttcg
     8701 gtgaacggct ggagtctctg tcggaatagc cgccagagtt gcctactggc c
//
LOCUS       DATMZD010000184         4385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_105856,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000184 DATMZD010000000
VERSION     DATMZD010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4385
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105856"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VN478_03795"
     CDS             <1..333
                     /locus_tag="VN478_03795"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HWQ21830.1"
                     /translation="LMALDLASGKEVWNHQFAPIKEPMTEGPDMGKEHDTYQLMTMTT
                     ARDALYVQDGGGLLAIFDPATGKELWSKRISQVVQKQTSVAGAYVMQVVDKGIAVIMS
                     NGTVSIWQ"
     gene            406..909
                     /locus_tag="VN478_03800"
     CDS             406..909
                     /locus_tag="VN478_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21831.1"
                     /translation="MSTSGLVKFIAVVTVLFLFISVGEYLLFRPSTTTPPSAADINAI
                     YRVVGEFGARLKDVVITAPDQDVMTAVDFNLKQLITDRLYQVFVQDKIRIPGRYVSSP
                     WPDRIEIDSGQMLDSGSYTIHGSQVVMTDDTVAHGGNAGETPITLTLKKVNGIWLIDD
                     VIGGPKT"
     gene            932..1861
                     /locus_tag="VN478_03805"
     CDS             932..1861
                     /locus_tag="VN478_03805"
                     /inference="COORDINATES: protein motif:HMM:NF027437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4829 domain-containing protein"
                     /protein_id="HWQ21832.1"
                     /translation="MRTTKSRSRSIAAAFISILLAVVLVAPGCALPRVTWSVDVRQGA
                     TMDIQVQSGATGYNGLVPAFVLDGTSPYTNDLSATFKVTDVIDTRTVWADTTAPVSLA
                     GQSSQEHISVILYLCWPLSDKADRFINGTVAYSATGYPDIIKGHTYTITGVLQQGWQS
                     IPLIYVPTAYDFKETTGDESFFYAPSDQFGQEPSSMAAKQVVEKFFTSWNGRNATAID
                     DCMAPKNKGMDWGFDALEYVKVISISERQPIEDPENTRVFMVMFDLKFKTNGPGIPSL
                     DEGKTYWNYILKRDNQGAPWLIYDWGGGGGYDS"
     gene            1911..3026
                     /locus_tag="VN478_03810"
     CDS             1911..3026
                     /locus_tag="VN478_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21833.1"
                     /translation="MKTRDSRCCSRIVAFACILLAVVLVATGCAPKVVPAEGPQVSGI
                     MLANVTASAYDTVPFVWSVRQNTLQSTGTSVLHANNTNGEAVLLWLSGSPLLAVQHWE
                     LMTATSKDLSLLVPPVGMQSASSVRARWYVQKENAFVAFGGQNGTDITITQQSIASGS
                     KAQTLTVPVPSGLQVDVLYGSGSIANGFVLFEAPQDKAPFVKPFSLWLLRISNGTTSW
                     VRCGDASAFGGGFYSGESPSFTRVGSLIYFTRGHTKIGCIDTAAASPSLTVPETLNTL
                     LAKVYSAGPTDTEAPLMAHLASDSDTLILECPDMYWNSVYYAVDASGTVLGSLRADKA
                     SIVSFDAKGQQGFTLKTPGSPGYVSFPSIDLFESPVS"
     gene            complement(3086..3322)
                     /locus_tag="VN478_03815"
     CDS             complement(3086..3322)
                     /locus_tag="VN478_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21834.1"
                     /translation="MIWYLALVIGVPAVLTVVLLVQDARRRKMTHMDDAESAYGAKRD
                     LYDETADIKTGVGRAMRDVDLYNGAKSINKPRGR"
     gene            complement(3540..>4385)
                     /locus_tag="VN478_03820"
     CDS             complement(3540..>4385)
                     /locus_tag="VN478_03820"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HWQ21835.1"
                     /translation="TWTDVSIFQAPMSGQTPPVAGACFLDKQTAFVATNSGTDNQPKI
                     VVYRTVDSGSTWDSTNIVTKLDWEKGDVGGLDVSFADASNGYLMIMGTPGAGQMGKSL
                     YRTVDGGKTFSFVGDITGMTDKAGTMSGIAGYPTGMTFSTPDVGFVTCSYSAYTYVLM
                     FKTTDAGKIWKLWSLPVPTAYQNLKPAQDFYADAYPPVFFGDHKSGVIMLDYVQDGTH
                     VMQSYRTADGGDTWTLGAVSNNSDVRTYSFIDATTGWGVDESGKLFTTTDGGATWTVI
                     ASAAK"
BASE COUNT          981 a         1201 c         1250 g          953 t
ORIGIN      
        1 ctcatggctc tggaccttgc cagcggcaaa gaagtatgga accatcagtt tgctccaatc
       61 aaagaaccta tgactgaggg tcctgacatg ggaaaagagc acgacacgta ccagcttatg
      121 accatgacaa cggcgaggga cgctctgtat gttcaggacg gcgggggatt gctggcgatc
      181 tttgatcctg caaccggcaa agagctgtgg agcaagcgca tctcgcaggt agtccagaag
      241 cagacttctg tcgccggtgc atacgtgatg caggtggttg acaagggaat tgccgtgatc
      301 atgtcgaacg gtacagtatc aatctggcag taacattctg atcaggcgca caagtgaaac
      361 cgcgggaagt tcatgccaac gcggcacctg ttggaggggc ataacatgag tactagcgga
      421 ttagtcaagt tcattgcagt agtcacagtg ctgtttcttt tcatctccgt cggcgaatac
      481 ctgctatttc gaccgtccac gacgactccc ccgagtgcag cggacatcaa cgcgatctac
      541 cgggttgtcg gcgagttcgg cgcacgcctg aaggatgtcg tcatcactgc acccgaccag
      601 gacgtgatga ctgcggtgga cttcaatctg aagcaactca ttactgaccg gctgtatcag
      661 gtgttcgtgc aggacaagat caggattcca ggaagatacg tttcaagtcc atggccggac
      721 cgtattgaga tcgactccgg gcagatgctg gacagcgggt cgtatacaat acatggaagc
      781 caggtcgtga tgacggacga taccgtggcc cacggcggca atgcaggtga gacccccatc
      841 acgcttacgc tcaagaaggt caacggcata tggcttattg acgacgtgat aggagggccg
      901 aaaacatgac gatgtgtcgg aggcatatga catgaggaca acaaaatcac gatcccgatc
      961 catagcagcc gcgttcataa gcatcctgct tgcagtggtt ctggtggcgc caggctgtgc
     1021 acttcccaga gtcacgtgga gcgttgatgt gcgacaaggt gccacgatgg acatacaggt
     1081 tcagagcggc gcgaccgggt ataacggtct cgttcccgcg tttgttctgg atggaacaag
     1141 tccctacaca aatgacctct cagctacgtt caaggtgacc gacgtcatcg acaccaggac
     1201 ggtctgggca gacaccaccg cgccagtcag tcttgcggga cagagctcac aagagcacat
     1261 atctgtcatt ctctacctct gttggccact ttcagacaag gcggacaggt tcatcaacgg
     1321 caccgttgcc tatagtgcca ctgggtatcc cgacatcatc aagggacaca catatacgat
     1381 tacgggagtc ctccagcagg ggtggcagag tatcccgctg atctatgttc ccactgcata
     1441 cgacttcaag gaaaccacgg gcgatgaatc cttcttctac gctccgagcg accagttcgg
     1501 gcaggaaccg tcttccatgg ctgcaaaaca ggtcgtcgag aagttcttca cgtcctggaa
     1561 tggcaggaac gccactgcca tagacgactg catggctccg aagaataagg ggatggattg
     1621 gggatttgac gcgttggaat acgtgaaggt gataagcatc agcgaacgac agccaataga
     1681 ggatcccgag aacacaagag tgttcatggt catgttcgac ctcaaattca agacaaatgg
     1741 acctggaatt ccctcattgg atgaaggaaa gacgtattgg aactacattc tcaagaggga
     1801 caatcagggc gcaccgtggc tcatctacga ctggggtgga ggcggtggat atgactccta
     1861 gagaatggtt gcggcagctt ccgcgatact cctgctggag ggtacatggc atgaagacaa
     1921 gagattcacg atgttgttcg aggatcgtcg ccttcgcatg catcctgctt gcagtagtat
     1981 tggtagcaac aggttgtgca ccgaaggtcg ttccagcgga gggaccgcag gtcagtggta
     2041 taatgctggc caacgtcacc gcgtccgcct acgatacggt cccattcgtg tggagtgtcc
     2101 ggcagaacac actgcagagc accggaacgt cggtcctgca tgcaaacaat acgaacggag
     2161 aggccgtgct gctctggctc tccggttcgc ctctgcttgc cgttcagcac tgggaactca
     2221 tgacggcaac gtcgaaggac ctcagccttc tcgtccctcc tgttggcatg cagtctgcca
     2281 gtagcgtgcg tgcacgatgg tacgtgcaga aggagaacgc tttcgtcgct tttggaggtc
     2341 agaacggcac agatataacg atcacgcagc agagcattgc ttctggttcc aaggcacaga
     2401 cgctgactgt tcccgtcccc tccggcctgc aggtggatgt cctatacggc agtggcagca
     2461 ttgccaatgg gtttgtcctt ttcgaagcac cgcaggacaa ggcgccattt gtcaaaccat
     2521 tctcgctttg gctacttcgc ataagcaatg gaacaacatc ctgggtgcgg tgcggcgatg
     2581 cctccgcgtt cggtggaggt ttctactccg gcgagagtcc aagcttcaca cgtgtcggtt
     2641 cgctcatcta ctttactcgc ggacacacga aaatcggttg catcgacaca gcagctgcgt
     2701 ccccatcgtt gacggtgccg gagacactga atacgcttct cgccaaggtc tacagtgctg
     2761 ggccgactga cactgaggcg cccctcatgg ctcacctggc aagcgatagc gacacattga
     2821 ttctcgagtg tcccgatatg tactggaatt ccgtctacta tgctgtggat gcatccggaa
     2881 cggtcctcgg aagcctgcgc gctgacaagg cttcgatcgt gagcttcgac gcgaaggggc
     2941 aacagggctt cacgctcaag acaccgggat ctccgggata cgtctcgttc ccttcaattg
     3001 accttttcga gtcccctgtg tcataggcgg agtactacga agggttcgtt acctgtctgt
     3061 cgaatttgag tcaaccctcc ggtggctatc ttcctcttgg cttgttgatg ctcttcgcgc
     3121 cgttgtacag gtctacgtcg cgcatcgcgc gaccgactcc agtcttgatg tctgcagtct
     3181 catcgtacaa gtcacgtttg gcgccgtagg cgctctccgc atcatccatg tgcgtcatct
     3241 tcctgcgccg tgcatcctgg acaagcagta cgacggtcag aacagccgga actcctatga
     3301 caagtgcaag ataccagatc atggtcgcct ccacgtggat tctaccatgt cgacgttaac
     3361 gcattgttaa cccgtgtgga aggggagatg tcttgtatgt tgcggcgttc ccgtcaatga
     3421 tcgcggcgcc gacctacaag cctatggcgt ccgcaggcgg cacgaaatca caaacccccc
     3481 ggcacaagtc gggggggttc actgcaatca gatcgctagc gtgaagactg gcatgactgc
     3541 tacttcgctg cggaagcgat gaccgtccac gtcgccccac cgtctgtggt cgtgaagagc
     3601 ttgccgcttt cgtcgacgcc ccagccggtc gtagcatcga tgaaggagta ggtcctgaca
     3661 tcggagttgt tggagacagc acccagtgtc caggtgtcac caccatcggc ggtgcggtat
     3721 gactgcatca cgtgcgtgcc gtcctgtacg tagtcgagca tgatgacacc gctcttgtgg
     3781 tctccaaaga acacaggggg ataggcatcc gcatagaagt cctgtgcagg cttcaagttc
     3841 tggtatgcag tgggaacggg caatgaccag agcttccaga tcttgcccgc gtccgtggtc
     3901 ttgaacatga ggacatatgt gtaggcactg taggaacagg tcacgaatcc aacgtcaggc
     3961 gtggagaagg tcatgcccgt cgggtacccg gcgatgccgc tcatcgttcc agccttgtca
     4021 gtcatgccag tgatgtctcc gacaaaggag aaggtcttgc cgccatcgac ggtccgatac
     4081 agagactttc ccatctgtcc ggctccgggc gtccccatga tcatgaggta gccgttcgac
     4141 gcgtcggcga aactgacgtc gaggcctcca acgtcaccct tctcccaatc cagcttcgtg
     4201 acgatattgg tgctgtccca ggtcgagcca ctgtcgaccg tgcggtagac cacgatcttc
     4261 ggctgattgt cagtgccgga attcgttgcg acgaacgccg tctgtttgtc gagaaagcag
     4321 gcgccagcca ctgggggcgt ctgcccggac atgggagcct gaaagatcga gacatctgtc
     4381 caggt
//
LOCUS       DATMZD010000185         3043 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_95975,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000185 DATMZD010000000
VERSION     DATMZD010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95975"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            270..1676
                     /locus_tag="VN478_03825"
     CDS             270..1676
                     /locus_tag="VN478_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HWQ21836.1"
                     /translation="MEEDRGGSQRSGLRRFFTALGNQVDFLGRSFRDGDARTRLSFVL
                     MGFGSLTRGQVAKGLYYFLSEIAFVAYMLLFGGRSLAGIVTLGTKLQHIEAIPGQVPK
                     VIRGDNSMLMLLYGVATVLLVVWFIATYFWNISSAAHAQRAAEKGEALPGLMAEARQY
                     LDVKFHITLMALPVTALLLFTVLPLVFMILIAFTNYDSSHQPPGSLFHWTGLYSFRAL
                     FSSGGVLGDTFWPILGWTLTWAIVATATNYTLGMLLAILINRRGVRFKGFFRTIFVLS
                     IAIPQFVSLLLMRNMLDDFGPVNGVLQQPGLVSKFLPFLTDATFARITVLVVNLWVGI
                     PYSMLITSGILLSIPKELYEAAAIDGASPFRMWTKIILPYVVFVTGPYLITQFIGNIN
                     NFNVIYLLTGGGPNSLNYYQAGKTDLLVTWLYKLTVNTRDFSYASAIGICVFVISAVL
                     SLIAYRNTKSYKKEETFQ"
     gene            1673..2554
                     /locus_tag="VN478_03830"
     CDS             1673..2554
                     /locus_tag="VN478_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWQ21837.1"
                     /translation="MSSRPFRHARGRLVAGNALAYVALAVLCLIWLVPIAWLVIISFS
                     KGVAGIPNYFWPKYGFTLQNYVKLFRDSGAASGFYFPRWFLNTLFVAICSCALTTAMT
                     LSVAYAVSRMRFRLRKPYLNVALILGMFPGFMSMIAVYYLLKAVGLTDTLVALILVYS
                     GGAGLGFYVAKGFFDAVPKEMDEAAMIDGATRSQIFWRITMPLSKPVVVYTILTSFLA
                     PWADFIFVNMIMGPTAYKNYTVALGLFRMIDREHITTYFSMFCAGAVLVSIPIVALFI
                     SMQKFYVEGITGGSVKG"
     gene            2590..>3043
                     /locus_tag="VN478_03835"
     CDS             2590..>3043
                     /locus_tag="VN478_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HWQ21838.1"
                     /translation="MPAWLRDAVFYEVYPQSFCDTNGDGIGDIEGIISKLDYIRSLGC
                     NAIWINPCFVSPFKDGGYDVADYKTVAPRYGTNDDLCRLFAAAHASGIRTLLDLVPGH
                     TSDEHPWFQKSLLPFTNEYTNRYIWTDSAWTAPRDFHCVSGRFNRNGNY"
BASE COUNT          572 a          941 c          858 g          672 t
ORIGIN      
        1 cttcaggcct ccatcaagca gatgagcaac tactggacgc cggccgccgc ccttggaaca
       61 agcatcggca acggcaagac gacgaatgcc acgctccaga aggatctgga cgcgatggtc
      121 aagcagattc tcgcagcact gcagtaagtc cttcgacagg aagcaatgac agacacagcg
      181 gcactgcggg tgcgtacgat gcacagccgt gccgctgtgt gtctgttcgg ccatcggcag
      241 cggtagaccc cgggaaaggg aggcggagca tggaagagga taggggagga tctcagcggt
      301 cagggctgcg caggttcttc actgcgcttg gaaaccaggt ggacttcctg ggccgcagct
      361 tccgcgatgg cgacgccagg acccggcttt cgtttgtcct gatgggcttt ggaagcctga
      421 cccgagggca ggtggccaag ggtctctact acttcctgag cgagatcgct tttgtcgcgt
      481 acatgctcct gtttggcggg cgttcgctgg caggcatcgt gacccttggc acgaagctgc
      541 agcacatcga ggcgatcccg ggtcaggtgc caaaggtgat ccgtggcgac aactccatgc
      601 tcatgctcct ctacggcgtc gcaactgtcc tgctggttgt ctggttcata gcgacctatt
      661 tctggaacat ctcctcggcg gcacacgcac aacgggccgc ggagaaggga gaagcgttgc
      721 cgggtctgat ggccgaagcg cgacagtacc tggatgtgaa gttccacatc acgctgatgg
      781 cgctgcccgt cactgccctc ctgctgttca cggtgctgcc cctggtgttc atgatcctga
      841 tagccttcac caactacgac agctcgcacc agccacccgg gtctctcttt cactggacgg
      901 ggctgtacag cttcagagcg ctgttctcca gcggcggcgt cctgggagac acattctggc
      961 cgatcctggg ctggactctc acgtgggcaa tcgtcgcgac tgccacgaac tacacgcttg
     1021 gcatgctgct cgccatcctc atcaaccgga gaggtgtccg gttcaagggc ttcttccgga
     1081 ccatctttgt gctctccatc gccataccgc agttcgtctc cctgctcctc atgaggaaca
     1141 tgctggatga cttcggtccc gttaatggcg ttctacagca gccgggactt gtgagcaagt
     1201 tcctgccctt tctgactgac gccacgttcg cgcggatcac ggtactcgtc gtcaacctgt
     1261 gggtcggcat cccctacagc atgctgatca caagcggaat cctgctgagc atcccgaagg
     1321 aactgtacga ggcggcggcg atcgacggcg cttcgccatt ccgcatgtgg acgaagatca
     1381 tcctgcccta tgtcgttttc gtaaccgggc cctacctgat cacgcagttc atcggaaaca
     1441 tcaacaactt caacgtcatc tacctgctaa cgggcggcgg tcccaacagt ctcaactact
     1501 accaggcggg caagactgac ctgcttgtga cctggctgta caaactcacg gtgaacacac
     1561 gcgatttcag ctacgcttct gccatcggca tctgtgtctt tgtcatctct gccgtcctgt
     1621 cgctgatcgc gtatcgcaac acgaagtcgt acaagaaaga ggagaccttc caatgagcag
     1681 tcgaccattc aggcatgcga ggggtcgcct cgtcgcgggc aatgcccttg cctacgtcgc
     1741 gctcgccgtg ctctgtctca tctggctggt ccctatcgcc tggctcgtca tcatctcgtt
     1801 cagtaaggga gtggccggta ttcccaacta cttctggccc aagtatggct tcacgctgca
     1861 aaactacgtc aagctgttca gggactccgg ggcagcatca gggttctact tcccccgctg
     1921 gttcctgaac accctgttcg tggcaatctg ctcatgtgct ctgacgacgg ccatgaccct
     1981 ctctgtggcg tacgcagttt cgcgcatgcg ctttcgtctg cgcaagccat acctgaacgt
     2041 ggcgctcata ctgggtatgt ttcccggttt catgtccatg atcgccgtgt actatctcct
     2101 gaaggcagtt ggcctgaccg acacgctggt ggctctcatt cttgtgtact ccgggggagc
     2161 tgggctgggg ttctatgttg caaagggctt cttcgacgcg gtcccgaagg agatggatga
     2221 ggcggcgatg atcgacggcg ccacgcggtc acaaatcttc tggcggatca ccatgcccct
     2281 gtccaagccc gtcgtcgtct acacgatcct gacctcgttc ctcgccccat gggcggactt
     2341 catcttcgtg aacatgatca tgggtcctac ggcctacaag aactacaccg ttgccctcgg
     2401 actcttccgc atgatcgacc gggaacacat cacgacgtac ttctcgatgt tctgcgcagg
     2461 cgccgtgctg gtgtcgattc ccatcgttgc cctcttcatc tccatgcaga agttctacgt
     2521 cgagggtatc acgggcggct cggtcaaggg ttgacgctgt ctcgtgagcc tcggtttcca
     2581 gaagatctga tgcctgcctg gctcagggat gctgtcttct atgaggtcta tccccagtcg
     2641 ttctgtgaca cgaacggcga tgggatcgga gacatcgagg gcatcatctc gaagttggac
     2701 tacatccgca gccttggctg caacgcgatc tggatcaacc cctgcttcgt ctcgccattc
     2761 aaggacggcg ggtacgacgt cgccgactac aagacggtgg cgccccgcta tgggacgaac
     2821 gacgacctct gccggctgtt cgcggcggcg cacgcctcgg gaatacgcac cctgctggac
     2881 ctcgtgccag ggcatacatc ggatgagcat ccatggttcc agaagagcct cctgccattc
     2941 acgaacgaat acacgaatcg ttacatctgg accgattccg cctggactgc cccgcgggac
     3001 ttccactgcg tcagcggcag attcaaccgg aacggcaact atc
//
LOCUS       DATMZD010000186         5245 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_45184,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000186 DATMZD010000000
VERSION     DATMZD010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5245
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45184"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="VN478_03840"
     CDS             <1..555
                     /locus_tag="VN478_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020267465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HWQ21839.1"
                     /translation="MLPHMTVINPCDVNEARKATLAVAAMQGPAFIRLGRESVPILTD
                     DATEFEIGKGITFEDGDDVTIAATGYMVYEALLAHEELKKQGIHARVLDIHTIKPIDR
                     ELLLKAARETGAVVTAEEHQVMGGFGSAVAEVLVQDCPVPMEFVGIRDRFGESGTPDE
                     LLTAFGCRHADIEAAVRKAIRRKK"
     gene            861..1934
                     /locus_tag="VN478_03845"
     CDS             861..1934
                     /locus_tag="VN478_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21840.1"
                     /translation="MTRYHEEQEGAAAASRARIRTALVICLLGLLLTVWCCAIALTHL
                     NQSVTAAVHGMDPGLELRKTDDLMNRVGSGDGFPWYTVYRGGKFEGDIYLFTFENAAS
                     RRSYLAPLFVSRQITWAVFFDDVQTVQRVWSLSPRFPTGLDARYSRLLDSLKGVACRD
                     LIRGGGLILPADGDELAVPARIAMGDLAISVYASRNSTADLNQLIAEDRTAGLVEHRG
                     FPLFSATTTAGRRIDTASLVGRNTLVLTAQPSCGSCVDEVVESIAKVKAVSSEPWNIV
                     LIMFAGSDGEGAQALMAGVGSSVDIIMDPERKLGNQVFMPDSPYMAVLDRNATVVYKG
                     GGDKIASLLAAIDAVQSGGVPQG"
     gene            2183..3232
                     /locus_tag="VN478_03850"
     CDS             2183..3232
                     /locus_tag="VN478_03850"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HWQ21841.1"
                     /translation="MEDRSNRTQQRSAVSPLRWAIPAVILVVAVAWGAGALMVRRTIS
                     PEALIHRYEPTATVKAADALTSSVTPTETYSTWAASSDKGGGSLYVVTQPNPFRVAWW
                     ARLLGVPPTVTVGTYIDDSYTIKGVVLMSPVSLPGAPSDLASYLDAWRETTMYTIVTT
                     TGGIKPAGGGALGEALTQSINGLAASIYTRDLGTAGLQRLVDQAHQITIGVRDTFPFF
                     AAKAADGTTFNVNSMKGRKFLVAFTRPTCGSCYEETMKLLNTVRDQKYGIAMVVFVFG
                     GDEFAPVQQFMREAPAGAIMISDGDASIAATIHQTLAPYVVLVDENMQVRYSGSANSP
                     GPIDDLLARFAKGTL"
     gene            3229..4434
                     /locus_tag="VN478_03855"
     CDS             3229..4434
                     /locus_tag="VN478_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21842.1"
                     /translation="MRSRSMRLAVLLSLIALAVLAGIGSVHAAGTAPAVAEATPIIYF
                     YTEGCSECHAVEQYLTSLQSKYDIVVYKKYDALDKAGVSIRASLDEQYGVPETQRGVA
                     PTIFVGDHALVREKAIKEGLDGALSSSTTSSRQHLLDAFTTAEKGGGTEPTEVFKTFS
                     VLTVIGAGLLDGINPCAFATLIFFISYLIMRSKSKRDILFVGLAFAAGVFLAYLAAGL
                     GLYQLVSRAKWFFALSKWFYLAIGIFAAVIAVLSVRDAWRARHGDLGDMTLQLPERNK
                     GIIHKLIRGQARTGFVATGAFLVAFPISLFEFMCTGQTYLPTIVLIFSQNVLRERALL
                     FLIIYNLLFIFPLVLITCVAYLGVTSERLVAWLQKNATTVKIATAVLMVALSTFLLVR
                     ALTLFGVIR"
     gene            4431..>5245
                     /locus_tag="VN478_03860"
     CDS             4431..>5245
                     /locus_tag="VN478_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21843.1"
                     /translation="MRESMQSRPGRLCAVIMLVGAMLGCSVPARAAETGGLSVLRKFP
                     AAVTVVGVGSVNGDPLTVVATRGAGLYATTAGAAIFRKLSPALDVMQISSIAVLGRDE
                     WLVGTDGDGLWKTTNAGSSFTFVKTLDCSRIARIVPDPAGSGRIFVASLCTGLHYSTD
                     GGLTWSGAGNGIQSKQVTDVVRLDASRIVAGTTTDGSSSASGVYFSTDNGKSYARMTS
                     PIPQVDALAWNGRSGTLFIAGGGKVVATADMGAHWKALTGVGALSGLGVSPSG"
BASE COUNT          984 a         1534 c         1673 g         1054 t
ORIGIN      
        1 atgttgcccc acatgaccgt catcaacccg tgcgacgtga atgaagcccg caaggccacg
       61 ctggcggtcg ctgccatgca gggccccgcg ttcatccgac tcggccgcga gtccgtgccg
      121 attctcacgg acgatgccac ggaattcgag atcggcaagg ggattacgtt cgaagacggc
      181 gacgacgtta cgatcgctgc gaccggatat atggtatatg aggcgttgct cgcgcacgaa
      241 gagctgaaga agcagggcat ccacgcgcgc gtcctcgata tccacaccat caagcccatc
      301 gaccgcgaac tcctgctgaa ggctgcccgc gagacgggcg cggtcgtgac ggccgaagag
      361 caccaggtca tgggagggtt tggctctgcg gttgcggagg tcctcgtgca ggattgcccc
      421 gtgcccatgg aattcgtcgg catcagagac cggtttggcg aatccggtac acccgacgaa
      481 ctgctgaccg ctttcggatg ccgccacgcc gacatcgagg cggctgtgcg caaggccatc
      541 cgaaggaaga aatagcagcc actcggcata gtacgtcttg aggcccggac cggaagtccg
      601 ggcctttgca tgcgcgagcg cctggtttcg caacagggat tgatcatcgc cacctccgta
      661 tcccacatca cacctgtcgt tggctcccag ctcgatagcg acgtgtcgac tggaacggct
      721 cgtggctcag ctgaatgtgg agacacgcag tcatgccatc ggcgcgtgct ggagtataat
      781 ccagccatgg cagccagccg ggcgccgacc gcagcggata cgtgcagcgc ggcgtcatga
      841 tccatgtcgg gaggatgacg atgactcgat atcacgaaga gcaagaaggg gccgcggctg
      901 cttctcgagc caggattcgc acggctctgg tgatctgtct cctcggcctt ctcctgactg
      961 tctggtgttg tgccattgcg ctgacccatc tgaatcagag cgtgacggca gcggtgcacg
     1021 ggatggaccc tggtctggag ctcaggaaga cggacgacct gatgaacagg gtgggttcag
     1081 gtgatggatt cccttggtac actgtgtaca gggggggcaa gttcgaggga gacatctacc
     1141 tgttcacctt cgagaacgcc gcctcgcgac ggtcctacct ggcaccgctg tttgtttccc
     1201 ggcagatcac gtgggccgtg ttctttgacg atgtccagac ggtgcagcga gtatggtcgc
     1261 tgtcaccgag gtttccgaca gggctggacg cacggtactc caggctcctg gacagtctga
     1321 agggcgttgc ctgccgtgat ctgatcaggg gaggcgggct gatcctgccg gcggacggcg
     1381 acgagctggc cgttcctgcg cggattgcca tgggtgattt ggccatttcg gtgtacgcca
     1441 gcaggaacag caccgcagac ctcaaccagc ttatcgcgga ggaccggaca gcaggtcttg
     1501 tggagcatag gggcttcccc ttgttcagtg cgaccacgac ggctggtcgg cgtatcgaca
     1561 cggcgagcct ggtggggcgc aacacactcg tcctgacggc acagccttcc tgtggttctt
     1621 gtgtcgacga agtcgtcgag tcgatcgcga aggtcaaagc agtcagtagc gaaccgtgga
     1681 acatcgtcct gatcatgttt gcagggagcg atggtgaagg ggctcaggcg cttatggccg
     1741 gagtgggttc ttcagtcgac atcatcatgg accccgagcg caagctgggg aatcaggtgt
     1801 tcatgccgga ttctccctac atggccgtgc tggacaggaa cgcgactgtc gtgtacaagg
     1861 gcggcgggga caagattgcg agcctgctcg ctgccatcga cgccgtacag tcgggtggcg
     1921 ttccacaggg atgagcccga ccgggatctg tgtctttacg tgtgtccagt cgtggcaggg
     1981 atcacccttc acccggttct gtgcctgcat cacaccttgt tctgccatag acattgaacg
     2041 agcagtgaag gcggcggacg cgccccggaa gcggtagccg gtaccattcg acaggatgag
     2101 cgccctcctg gctgtttgac ggttcagggg ctgccgatac actatgcagg taccgcagaa
     2161 gtcaatcgtg caaggagtcc acatggaaga caggagcaac cgaacacaac agaggtcagc
     2221 cgtcagcccg ttgcgctggg cgatccccgc cgtcatcctc gttgttgccg tggcatgggg
     2281 cgcgggagcc ctgatggtca ggcggacgat ttcgccggaa gcgctgatcc atcgctatga
     2341 acccacagcg accgtgaaag ccgccgatgc gctgacaagc tcagtcacgc cgaccgaaac
     2401 ctacagcacg tgggcggcca gctccgacaa gggaggagga tcgctgtacg tggttactca
     2461 gcccaatccg tttcgcgtag cctggtgggc ccgactactg ggggttccgc ccactgtgac
     2521 ggtaggcacg tacatcgatg acagctacac catcaaggga gtcgtcctga tgtctcccgt
     2581 cagcctgccc ggagccccgt cagacctggc ctcatacctt gacgcatggc gagagacgac
     2641 gatgtatacg atcgtcacga cgaccggcgg tatcaagccg gcaggaggtg gcgctctcgg
     2701 ggaggctctc acacagtcaa tcaacggcct tgccgcctcg atctacactc gcgacctggg
     2761 gactgcagga ctgcagcgtc tcgtcgatca ggctcaccag ataacgatcg gtgtgcgtga
     2821 tacctttccc ttctttgcgg ccaaggcggc agatggcaca acgttcaacg tcaacagcat
     2881 gaagggcagg aagttcctgg tcgcgttcac acggccgacg tgcggctcgt gctacgagga
     2941 gacgatgaag ttgctcaaca ccgtccgcga ccaaaagtac ggcattgcaa tggtcgtatt
     3001 cgtctttggt ggagacgagt tcgctcccgt acagcaattc atgcgcgagg cgccggctgg
     3061 agccatcatg atctcggacg gtgatgcttc catcgcggcc acgattcatc agacgttggc
     3121 gccatacgtc gtgctggtgg acgagaacat gcaggtcagg tactccggca gtgccaattc
     3181 gccggggccc atcgacgacc tgttggccag gtttgccaag gggacgctgt gagaagccgt
     3241 tcaatgagat tggcggtcct cctgtcgctg atagccctcg ccgtactggc gggcattggg
     3301 tccgtccacg cagctggcac ggcgcctgcg gtggccgagg cgacgccgat catctacttc
     3361 tataccgaag ggtgcagcga atgccatgcg gtcgagcagt accttacgtc tctgcagtcc
     3421 aagtacgaca ttgttgtcta caaaaagtac gacgcgctgg acaaggcagg ggtcagcatc
     3481 agggcgtctc ttgacgagca atatggagtc ccggagacgc agcgcggggt ggcgcccacc
     3541 atcttcgtgg gcgatcatgc gttggtgcgc gagaaggcca tcaaggaggg acttgatggc
     3601 gcgctgagct cttcgacgac ctcgtcgcgc cagcacctgc tcgacgcctt cacaacagcc
     3661 gagaagggtg gaggaactga gcccaccgaa gtcttcaaga cgttcagtgt gctgacagtc
     3721 atcggcgcgg gtctcctcga cggcattaac ccgtgtgcat ttgccacgct gatcttcttc
     3781 atcagctatc tcatcatgcg cagcaagtcc aagcgggata tcctcttcgt cggactggcg
     3841 tttgccgccg gcgtcttcct ggcttatctt gccgccggcc tggggctgta ccaactggtg
     3901 tcgcgcgcca agtggttctt cgcgctgagc aagtggttct accttgccat cggcatcttc
     3961 gccgccgtta tcgcggtgtt gtccgtgcgc gacgcatggc gagcgcggca cggtgacctg
     4021 ggcgacatga cgctgcaact tccggagcgg aacaagggca tcatccacaa gctcattcgc
     4081 ggccaggctc gcacggggtt cgtcgcgaca ggcgccttcc tcgttgcatt cccgatatca
     4141 ctattcgagt tcatgtgcac ggggcagaca tacttgccga ccatcgtcct catcttcagt
     4201 cagaacgtgc tcagggagcg cgctctgctt tttctgatca tctacaatct gctgttcatc
     4261 ttcccactcg tgctcattac gtgtgtcgcc tatctcggcg tcacaagtga gcgcctcgtg
     4321 gcgtggctgc agaagaatgc gacaaccgtc aagattgcga ccgcggtgtt gatggtagct
     4381 ctgtcaacct tcctgctggt ccgtgcactg accctgtttg gagtgatcag atgagagagt
     4441 ccatgcagtc tcgtccaggg cgcctgtgcg ccgtcatcat gctcgtgggg gccatgttgg
     4501 gctgcagtgt ccccgctcgg gcggcagaga cgggtggcct gtctgtcctg cggaagttcc
     4561 cggcggcagt gacggtcgtc ggcgtaggta gcgtgaacgg agatccattg actgtcgttg
     4621 cgacacgggg cgccgggctg tacgccacga ccgccggggc cgccatcttt cgtaagctct
     4681 ctcctgcgct tgacgtcatg cagatcagct cgatagcggt acttggacgc gacgaatggc
     4741 tcgtcggaac agatggagac gggctctgga agacgacgaa tgccggcagc agcttcacct
     4801 ttgtcaagac gctcgattgt tcccgcatcg ctcgcatcgt gcctgatccc gcagggtctg
     4861 gcaggatctt tgtagcatcg ctgtgcactg gcctgcacta ctcaacggac ggtggtctga
     4921 cgtggtccgg cgcagggaat ggcatccagt ccaaacaagt gaccgatgtg gttcgcctcg
     4981 acgccagccg cattgttgca gggacgacga cggacggatc gtcgtctgcg tccggtgtgt
     5041 acttctcgac ggacaatggc aagtcgtacg cgaggatgac cagtcccata ccgcaggttg
     5101 acgcccttgc atggaatggc cggagtggga cgctgttcat agccggcggt ggcaaggttg
     5161 tcgctacggc cgacatgggt gctcactgga aggcgttgac gggcgtgggg gccttgagcg
     5221 gcctgggagt gagtccgtcg ggatg
//
LOCUS       DATMZD010000187         3396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_105956,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000187 DATMZD010000000
VERSION     DATMZD010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105956"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VN478_03865"
     CDS             complement(<1..261)
                     /locus_tag="VN478_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014138711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase CobQ"
                     /protein_id="HWQ21844.1"
                     /translation="MIVGSCSSAGKSLLVTALCRLFSRQGVRVAPFKAQNLSNNAAVS
                     TDGAEIGRAQALQAEAAGIAPAADMNPLLLKPGADGSCQVVLM"
     gene            complement(504..899)
                     /locus_tag="VN478_03870"
     CDS             complement(504..899)
                     /locus_tag="VN478_03870"
                     /inference="COORDINATES: protein motif:HMM:NF020950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HWQ21845.1"
                     /translation="MKRRMLAVVIVVLAVAAVVVTLVLTGASGGDKITGDTFETTVGK
                     TFTIALDANATTGYSWRQTIKDESVVAYVDNAYVAEARDPQVVGGGGTDTFTFKAVGK
                     GTTTITLTYARPWESTTAAQTRTIVITVK"
     gene            1040..1366
                     /locus_tag="VN478_03875"
     CDS             1040..1366
                     /locus_tag="VN478_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWQ21846.1"
                     /translation="MATNELYGKQARLVDLVAVQPGAVVSKTLIERPTGTVTLFAFDK
                     GQKLSEHTAPFDALVTALQGSMDVILDGVHHTLAPGDAIVMPAGVVHAVDALEPCVWL
                     LTMVRS"
     gene            1376..2494
                     /gene="corA"
                     /locus_tag="VN478_03880"
     CDS             1376..2494
                     /gene="corA"
                     /locus_tag="VN478_03880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HWQ21847.1"
                     /translation="MPDGDSPDWSRGTPESASRTRSRHEPGVGSGGSVPATIPAVPPD
                     DVVDPPTRIRVLWYDQDHCEEEESDDVDICKAFSARAGTTWIDVTGVSDSTVLQELGR
                     TYHLHPLALEDVQNLRERPKVETYQGQKFVVFRLVRLGDAVTVEQISAFLVGSTLITM
                     QEDPVDPWEAIRRSIREGAGRERSEGADYLLYSLIDVAIDAIFPVIEQLGDQLEDLET
                     AVIDKPTPETLESIFALKKELLMLRKTVWPEREVVLGLQQDLSGLIKDETKLYLRDAY
                     DHSIQLMDMLETYRDLASNMVEAYLSSLSNRLNDIMKTLTIISTIFVPLTFIVGLYGM
                     NFRNFPEIEWKYGYLFVWAIMAVVVVFILRYYKRKHWM"
     gene            2607..>3396
                     /locus_tag="VN478_03885"
     CDS             2607..>3396
                     /locus_tag="VN478_03885"
                     /inference="COORDINATES: protein motif:HMM:NF036305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HWQ21848.1"
                     /translation="MDENVARFLEERLGGEQYAKLGKIPNARLYDFIAHFIKLLDPAS
                     VFVCDDSTQDLSYISEKAIRDGEEQRLAMNGHTIHFDSPLDQGRDKEHTNILVDPGEE
                     MGPQISTRDRTEGLKDIGEVMAGIMHGRQMLIVFATLGPNGSEFAIPGVQLTDSPYVA
                     HSEKLLYRPGYEEFIRQGEKAHFFKFVHSEGELTDSKTTKNIDKRRIYIDLKDDIVYS
                     ANTQYAGNTLGMKKMAMRLAIHRGSQEGWLCEHMLVMGIHGPGGR"
BASE COUNT          703 a          991 c         1008 g          694 t
ORIGIN      
        1 catgaggacg acctgacagc tcccgtccgc gccgggcttg agcaggaggg gattcatgtc
       61 cgcagcgggt gcgatgccgg ccgcttcagc ctggagagcc tgggcacggc cgatctctgc
      121 tccgtccgtg ctgacagctg cgttgttgga gaggttctga gccttgaagg gagcgacgcg
      181 gacgccctgc ctcgaaaaca ggcggcacaa cgcagtcacc agcaggctct tgcctgccga
      241 cgagcaggat cctacgatca tcagtgtctt gccttgctgt gatttcacag tctctcccca
      301 tggcgatctc gcgaaagtgc tggatgcata tcttccacga gcgagcacat cataccctta
      361 caagtgccgc agctcaacct gctgtgaaag ggacgttcga gatgtccaga catgggcgaa
      421 accccgaggc gggacgtgac tggtatatga ctatgccccg cagcggcagg ctgcggggca
      481 tagtcggacc cgtaaaggcc cttctacttg acggtgatga cgatggtcct ggtctgagcg
      541 gcagtcgtgc tctcccaggg gcgtgcatag gtcagagtga tcgtcgtcgt tcccttacct
      601 acggctttga aggtgaatgt gtcggtacca ccacctccga cgacctgggg atctcgagct
      661 tccgcgacat aagcattgtc gacgtatgcg actacgctct cgtccttgat ggtctggcgc
      721 cagctgtacc cggtcgtggc atttgcgtcc agagcgatcg taaacgtctt cccgacggtt
      781 gtctcgaacg tatctccagt gatcttgtct ccaccggaag ctccagtcag aacaagagtg
      841 acgactacgg cggcaaccgc cagaacaacg atgacaacag ccagcattct cctcttcatg
      901 atctcatctc cttgagtcgg tcggggtcac cgaccgtcac ccttaggacg aaacaagttc
      961 cgacagggtt cccgcgaacg ttcgcgctga ctgttgcccc ataccgccta tcctgataca
     1021 atcgagctgg aggtgttcca tggcgacaaa cgaactctac ggcaagcagg cgcggctggt
     1081 cgatctggtc gcggttcagc ccggggctgt tgtcagcaag acgctgatcg aacgcccgac
     1141 ggggactgtc acgttgtttg cgtttgacaa gggacagaag ctgagcgagc acacggctcc
     1201 attcgatgca ctggtgacgg ccctgcaggg gagcatggac gtcattctgg atggtgtgca
     1261 tcacaccctc gctcctgggg acgccatcgt catgccggca ggcgtcgtac atgccgtcga
     1321 tgcgctggag ccatgtgtgt ggcttctcac gatggtacgc tcgtgacggc ctggcatgcc
     1381 tgacggcgac tcacccgact ggagccgggg cacgccagag tcggcttcca ggaccaggtc
     1441 cagacacgaa cccggcgtcg gttcaggtgg ctcggttcct gctaccatac cggctgtgcc
     1501 gcctgacgac gtggtggatc cgccgactcg gatccgagtc ctgtggtatg atcaggacca
     1561 ctgcgaggaa gaggagtcag atgacgtcga catctgcaag gcattctccg cccgggcggg
     1621 caccacctgg atcgacgtta ccggtgtcag cgactcgact gtcctgcagg aactgggccg
     1681 cacctaccat ctgcaccctc ttgcgctcga agacgttcag aaccttcgag agcgccccaa
     1741 ggtggagaca taccagggcc agaagttcgt tgtgttcagg cttgtccgcc tcggcgacgc
     1801 cgtcactgtc gaacagatct cagccttcct tgtgggttcc acgctcatca ccatgcagga
     1861 ggaccccgtt gatccctggg aggcaatacg cagatctatt cgagagggag ctggcagaga
     1921 gcgatccgag ggagccgact acctgctcta ctcgctcatt gacgttgcga tcgacgcgat
     1981 atttcccgtc atcgaacagc tgggggatca gctggaagac ctcgagactg cagtcatcga
     2041 caagccgacg cccgagacgc tggaatccat cttcgctctg aagaaggagc tgctcatgct
     2101 gcgcaagacc gtgtggcctg aacgcgaggt cgttctcggg ctgcagcagg acctttcagg
     2161 gctcatcaag gacgagacca aactgtatct gcgtgacgcc tacgaccatt cgatccagct
     2221 gatggacatg cttgagacct accgggatct tgcctcgaac atggtggaag cctatctctc
     2281 gtctctcagc aacagactga acgacatcat gaaaacactg acgatcatct cgacgatctt
     2341 tgtgccgttg acgttcatcg tcggcctgta cggcatgaac ttccgcaact tcccggaaat
     2401 cgagtggaag tacggctatc tgtttgtgtg ggcaatcatg gccgttgtag tcgtcttcat
     2461 cctcaggtac tacaagcgca aacactggat gtgacttcgt cgtccggctt ccgcccgggc
     2521 tggcagcctg tgtcttttct cacaatggtg tagaatgtcc gtgtgacaaa aacggccgca
     2581 aggccagcat cgatggaggt gtctgcatgg acgaaaacgt cgccaggttt ctggaggaac
     2641 ggctcggcgg ggagcagtat gcgaagctcg gcaagatccc caatgcgagg ttgtacgatt
     2701 tcattgctca cttcatcaag ttgctggatc cagcgagtgt cttcgtctgt gatgattcga
     2761 ctcaggacct gtcctacatc agcgagaaag ccattcgcga cggtgaggaa cagcgcctgg
     2821 caatgaatgg ccacacgatc cacttcgatt cgccgctcga tcagggccgc gacaaggaac
     2881 acacgaacat ccttgtggac cccggcgaag agatgggtcc gcagatctcc acgcgtgacc
     2941 gtaccgaggg cctcaaggac atcggcgagg tcatggccgg catcatgcac gggcgtcaga
     3001 tgctgatcgt ctttgccacg ctgggaccaa acggttcaga gtttgccatc cccggggttc
     3061 agctgactga cagcccctat gtcgcacatt ctgagaaact gctgtaccgg cctggctacg
     3121 aggagttcat tcgccagggc gagaaggcac acttcttcaa gttcgtgcat tcggagggcg
     3181 agctgaccga cagcaagacg acgaagaaca tcgacaagcg ccgtatctac atcgacttga
     3241 aggatgacat cgtctacagc gcgaatacgc agtatgccgg caacacgctg ggtatgaaga
     3301 agatggccat gcgtctcgcg attcaccgag ggtcgcagga aggctggctg tgtgagcaca
     3361 tgctcgtcat gggcatccac gggccgggcg ggcgtg
//
LOCUS       DATMZD010000188         4396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_36337,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000188 DATMZD010000000
VERSION     DATMZD010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36337"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="VN478_03890"
     CDS             <1..644
                     /locus_tag="VN478_03890"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HWQ21849.1"
                     /translation="IAERKSRTVTFDLSQNGRDSIGMLCGGQVTLYIEYVGSRPSLYV
                     YGAGHVGRFLATFASLVGFEVTVLDDRPEFATPARIPDAQHFLTGDFVGLVQTTAYGL
                     QGYHVILTDRHVSDERVLKALLERKVDNRYIGMIGSRSKTLEVFRHLAASGVSIEELA
                     CVYAPVGIDHGGQTAEEIALAICAELVAVRHDRILADSMKNRANIVRLLESKP"
     gene            641..1522
                     /locus_tag="VN478_03895"
     CDS             641..1522
                     /locus_tag="VN478_03895"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HWQ21850.1"
                     /translation="MIKELEYHSARDLKEALELAEHFGPRKRFLSGGTDVMVRLKEGL
                     VKEDVIVDLSRVAELRFVREEAGAVHVGAGATHADVSASPVIQQHGRALALAASRIGG
                     PQIRNMGTIGGNVANASPAGDTIPALIVLDAVVCIASRTGTREVPIIDFFRGPGRSVL
                     AADELVTEFHFPSTGSTELTGFAKLGSRQAMTISTANTAFYFRIGDNRHIAEARIAFG
                     SVAPTVVRAHKTEQLFCQLPPVLSWDAIRGAAQMAWKEVAPIDDLRATAVYRKEAVVG
                     LLAEAAYEALASCWNEG"
     gene            1512..1745
                     /locus_tag="VN478_03900"
     CDS             1512..1745
                     /locus_tag="VN478_03900"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HWQ21851.1"
                     /translation="MKVKIKLFQDYKQYHDTPEYELELPVGTTAGEIVRDLGIKGPEL
                     AYLILILKGKRVYDEYLLQDGDELVFAAPIGGG"
     gene            1766..4021
                     /locus_tag="VN478_03905"
     CDS             1766..4021
                     /locus_tag="VN478_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014452761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HWQ21852.1"
                     /translation="MDDIRFEQMDRIDAQGKACGTTAYMSDLSFPGMLYAWFVRSKYP
                     HALIKSVDVSKAVALDGVVAVLTAADVTGFNGFGIVDPDMPVICGDKVRYMGDTVALV
                     AATTERIAEQAAALVEVDYEVLPVVDDPVEALKPDAPKVHDKGNVCLHTLIAKGDVEQ
                     GLREADVVVEHEYRTPRQAHTPLETESGVAVPEADGSLSMYAGSQYAFRDQLQLARIL
                     GMNPRMIHVYSNPVGGGFGGKDDLTIQGGIALLAMRTGKPVKVVLSREESLVSGWKRH
                     PFIIRVKTGFKSDGTMLANDVVCYEDKGAYTSLGGPVLNLALEHASSLYRVPNTRHEG
                     WAVFTNNGNCGAFRGFGVPQVLFALESNMDEAAERLGLDPVTIRRRNVLHQGEVSPIG
                     HTLRTGVSMDLVLDAIEKSDLWQHRDEYRKADRPWLRRGVALALAPQGAGLGVGIPDY
                     GGAYIDLDRDGSFTLRIGLVEYGQGTHTGFTQMAAGLLHVAPSRVRIVAGQTEGSVDA
                     GPATASRSMYTASNAIAAAFRDFAVKVAGALDTKYHTGADAVCEARDGGMTTPAGFRT
                     YEELGQEFSQESPLRGEGYFVWPTADREIPGAAGLPHWIYSFAAQVALVEVNTMTGET
                     RLLRVATALDTGHTVNLQQVEGQIDGGVAQGEGFALFEDTVITKGQIRTKNFSTYIIP
                     TVQDVPKHEYIVLENPEGTSALGVRGIGEVVMVPVIPAVALAIHDATGIWVRQLPATP
                     ERVYRLLHPDL"
BASE COUNT          875 a         1261 c         1416 g          844 t
ORIGIN      
        1 ctattgcgga aaggaagtcg cggaccgtca ccttcgatct gagtcagaac ggcagggaca
       61 gcatcggcat gttgtgcggc ggtcaggtga cgctgtacat cgagtacgtg ggcagccgtc
      121 cgtcgctgta tgtctatggg gccgggcacg tcggccggtt tctggcgacg tttgcctctc
      181 tggtcggctt cgaggtcact gtcctcgacg atcgaccgga gttcgctact ccggccagga
      241 tcccggatgc ccagcacttc ctgacaggtg acttcgtggg actggtccag acgacggcct
      301 atggactgca gggataccac gtcatcctca ctgacaggca tgtgagcgac gagcgagtgc
      361 tcaaggcgct cctggagcgg aaggtcgaca atcgctacat cggcatgatc ggctcgcggt
      421 cgaagacgct tgaagtcttc cggcacctcg cggcctcagg ggtgagtatt gaagagcttg
      481 catgcgtcta tgctccagtc ggcatagacc atggtggaca gaccgcagag gaaatagcac
      541 ttgccatctg cgctgaactt gtggcggttc gtcacgaccg catactggcc gattcaatga
      601 agaacagagc caacatcgtc agactcctgg agagcaaacc atgatcaagg aactcgagta
      661 ccattctgcg cgggacttga aggaagccct ggaactggct gaacactttg gaccacgcaa
      721 gcgcttcctg tccggcggca ccgacgtgat ggtacgcctc aaggaaggac tcgtcaagga
      781 agacgtcatc gttgacctct ctcgcgtggc ggaactccgc ttcgtgcgcg aggaggcggg
      841 tgctgtccat gtcggtgcgg gcgctaccca tgcggacgtc agcgcttcgc ccgtcattca
      901 gcagcacggc cgtgccctgg ctctggccgc ctccaggatc ggcggtcccc agatccgcaa
      961 catggggacg atcggcggca acgtagccaa tgcctctccg gcgggcgaca cgatcccggc
     1021 actcatcgtt ctcgacgccg tcgtctgcat cgccagccga acaggcacac gcgaagttcc
     1081 catcatcgac ttcttcagag gaccgggacg atctgtgctt gccgcagatg aactcgtgac
     1141 cgaattccac tttccctcta ccggttcgac cgagctgaca ggatttgcca agctgggttc
     1201 acggcaggcg atgacgattt ccaccgccaa taccgctttc tacttcagga tcggcgacaa
     1261 tcgtcatatc gccgaggccc gcatcgcctt tggctcagtc gctcccaccg tggtccgggc
     1321 gcacaagacg gaacagctgt tctgtcagct tcccccggta ttgtcgtggg atgccatccg
     1381 gggcgctgcc cagatggcgt ggaaggaagt agcgcccatc gacgatctga gggcgaccgc
     1441 cgtctatcgc aaggaagctg tcgtcgggct tcttgccgag gccgcctacg aggcgctcgc
     1501 ctcctgctgg aatgaaggtt aagatcaagc tctttcagga ctacaaacag tatcacgaca
     1561 cacccgagta cgagctggaa ctgccggtag ggacgacagc cggcgagatc gtaagggatc
     1621 tggggatcaa ggggcctgaa ctcgcatatc tcatcctcat tctgaagggc aagcgcgtgt
     1681 acgacgagta cctccttcag gacggcgacg agcttgtgtt cgcagcccct atcggtggtg
     1741 gctgagcgtg gaaaggagcg atctgatgga cgatatccgt tttgagcaga tggacaggat
     1801 cgacgcgcag ggcaaggcct gcggcaccac agcgtatatg tccgacctct ccttcccggg
     1861 gatgctgtac gcatggtttg tgcgcagcaa atacccgcat gccttgatca agagcgtcga
     1921 cgtgagcaag gcagtggcac tggacggcgt cgtcgccgtg ctgacggcgg cggacgtgac
     1981 ggggttcaac gggttcggga tcgtcgatcc agatatgccc gtcatctgtg gtgacaaggt
     2041 gcggtacatg ggcgataccg ttgcactggt ggctgccacg accgaacgga tcgctgagca
     2101 ggcggcggcc ctggtcgagg tggactacga agtcctgccg gtcgtcgacg atcccgtcga
     2161 agccctgaag cctgatgccc ccaaggttca tgacaagggg aacgtctgcc tgcacaccct
     2221 cattgcaaaa ggggatgtgg aacaggggct gcgcgaggcg gacgttgtcg tggagcacga
     2281 atatcggacg ccgcgtcagg cccacacgcc gctggagacg gagtccggcg tggccgtacc
     2341 ggaagccgac ggctcactgt cgatgtatgc tggttcccag tacgcgttcc gggatcagct
     2401 gcagcttgcc cgcatcctcg ggatgaaccc ccggatgatc catgtctaca gcaaccctgt
     2461 gggtggcggt tttggcggca aggatgatct gaccatccag ggtggcatcg ccctgcttgc
     2521 catgaggacg ggcaagcccg tgaaggtcgt gctctcgcgc gaggagtcgc tggtgtctgg
     2581 ctggaagcgg catcccttca tcatacgtgt gaaaaccggg ttcaagagcg acggtacgat
     2641 gcttgccaac gatgtggtct gctacgagga caagggggcc tacacgtcgc ttgggggccc
     2701 agtcctcaac ctggccctcg aacacgcatc gtccctctac cgcgtcccca acacgcgaca
     2761 tgagggctgg gcggtgttca cgaacaacgg caactgtggc gcgttccgtg gtttcggcgt
     2821 tccgcaggtc ctgtttgcgc tggaatccaa catggatgag gcggcagaga gactggggct
     2881 ggatcccgtc acgatacggc gcaggaatgt gctgcaccag ggcgaggttt cgcctatcgg
     2941 tcatactctg cgcacgggcg tctccatgga ccttgtcctt gacgccatcg agaagtctga
     3001 cctgtggcag catcgcgacg aatacaggaa ggccgacagg ccatggctcc ggaggggagt
     3061 ggcgctcgcg ctggctccgc agggagccgg gctcggggtc ggcatccctg actatggcgg
     3121 tgcatacatc gatctcgacc gggacggcag cttcacgctc cgcatcggac ttgtggaata
     3181 tggtcagggc acgcataccg ggttcacgca gatggctgca ggtcttctcc atgtggctcc
     3241 ttcacgggtt cgcatcgtag ccggccagac cgagggttcg gtcgacgcgg gacccgcaac
     3301 cgcctcacgc tcgatgtaca cggcctcaaa cgccattgca gcagcgttcc gggatttcgc
     3361 cgtcaaagta gccggcgctc tggatacaaa ataccatact ggagcggacg cagtctgtga
     3421 ggccagggat ggaggcatga cgacaccggc aggcttccgg acatacgagg aactcggtca
     3481 ggagttctcg caggaatcgc cgttgcgtgg ggagggctac ttcgtgtggc cgacggctga
     3541 cagggagatt ccgggcgctg cggggttgcc gcactggatc tacagctttg cagcgcaggt
     3601 ggccctggtc gaggtgaata ccatgaccgg tgagacccgc ctgctcaggg tcgcaactgc
     3661 gcttgacaca gggcacacag tgaacctcca gcaggtcgag ggccagatcg acggcggggt
     3721 cgcgcagggc gagggattcg ctctatttga ggacacggtg atcacgaaag ggcaaatccg
     3781 cacgaagaac ttctccacct atatcatccc gaccgtccag gatgtgccga agcatgagta
     3841 catcgtcctg gagaacccgg aggggaccag cgctctgggc gtccggggta ttggggaagt
     3901 agtcatggtc cccgtcatcc ctgccgttgc cctggcaatc catgatgcca ccgggatctg
     3961 ggtgcgtcag ttgcccgcga cgcctgagcg cgtctatcga cttctgcacc cggacttgta
     4021 gagagaactt gtgttgatgg acatggcttc cccgcctgct ctggagtggg cggggaagcc
     4081 atgtcgggaa ccagccgacc agaaggaacg tctaatgaga tggccgatgt gaccggacat
     4141 cgagtccact gctgcaaatg aagtgtgaag gcgacccgac agggccacag atgttgcagc
     4201 gagcgtttat gacgggctgc aagagacgga ttagtgaaca tattagtgtt gcaaaatacg
     4261 gtagtctgct cagacttgag tcaggatatg actgtagcgg tgcagtaggg ctctgtcgca
     4321 ccgcgcgagc gataagctgg aaagatatga ggaggcacga atgaagagag ttttgtcact
     4381 attgctggta ctggca
//
LOCUS       DATMZD010000189         3632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_136719,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000189 DATMZD010000000
VERSION     DATMZD010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136719"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /locus_tag="VN478_03910"
     CDS             <1..852
                     /locus_tag="VN478_03910"
                     /inference="COORDINATES: protein motif:HMM:NF013844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HWQ21853.1"
                     /translation="LAHDELSNEHKATTELMSRIVTDSQEISQTKDSLNVNQQELEKL
                     RADYEAKLTQLEANEKQELKTAHAEAQRIVDDVSRRMDDLMKQYQDSIKNQPQIAHDA
                     KHEVETTRDELIQKAESYEEKPPFTPVEEILPGDLVIITSVNKQAIVIDVMKDKRKLV
                     LQSGSMRTTVPEAQVRKLSEPELNSYQNRAMQYSGAREPTLPFVPMKIDLHGSRVDEA
                     LEKLDKYIDVAYLSGLSFVYICHGKGSGILRKQIHEFLKTLPSIDHFSFSSPEEGGDG
                     VTIAYFK"
     gene            864..3374
                     /locus_tag="VN478_03915"
     CDS             864..3374
                     /locus_tag="VN478_03915"
                     /inference="COORDINATES: protein motif:HMM:NF013122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HWQ21854.1"
                     /translation="MKNEQELVFFDLETTGLDQASDQIIEIGAVKVRGGKEVGRFEQF
                     CRLREGGRLPEFISALTGITTLDLEPAESVDQVVDQFLEFAGSSPLLAHNSNFDEGFL
                     RRARHGQLSNAVYDTLELARVFFPDLQSHSLVALVDSLAIKKEEAHRALGDSLSLFRF
                     YRKLKDKISQSALPDSIVRRIQFLYPVIADLGLLDNTDTRVAVGPSQMPGLETVFTSL
                     DYSRLPHLKALRPGISSAALSPEMVFLASGRDVLLQCEPQSLRARYLADVQASSRPGA
                     VLLRAPAAIEPLLEDARPGDQRPLWISQESPNNLVCLMLLDEASRSPTMRDEFGFELS
                     ALLVYEWETRSVFIPAMPLFLKKSRLINHISAVHCQIESSCPFHDVCPVRESLARARI
                     AHLHVTDLAEAPHVLRELGESTSVLVSSPETEKLMSDVLDVPEVQVASITLRIIAGIA
                     GERRQVPGLEVVADLAARARTLLDELYLRVTHQENAGVRGRIAIGEALWSAGEFAALR
                     STAEALVDAFMNAGESLPAAMRASYLMQCQDLASVLDNADNPAYAVWMERQGQEITLA
                     STRTILAARVRACNSGAQFFLAGTSVCPAGDQRFLPETFGLMDPEPAAVVVDGEPEVP
                     RVPTLVTLHLPKPNESGFEKEGARLIAETMRRFPGKSMVASNSLETLRRMQSFLSREA
                     GLSDYSLLLPKNGHTRTQLLAQFSGQDRAILLVPFDYLSFGDIVSAKFLFVTKLQFPN
                     SFLPTVTRLKQALKERRGDPFRQFDLPYALTLLQYTLHQMDPRTLRSVFMLDNRSFGS
                     AYADRIRTVLPTPSLFLPMENQESLLQHLDRWMRNQTL"
     gene            3446..>3632
                     /locus_tag="VN478_03920"
     CDS             3446..>3632
                     /locus_tag="VN478_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U34"
                     /protein_id="HWQ21855.1"
                     /translation="MCDTLVALAPMTLNGHTMLAKNSDREPNEAQLLTVMPRRTHGEA
                     TLQTTYVEVDQVRETNAC"
BASE COUNT          790 a         1113 c         1019 g          710 t
ORIGIN      
        1 cttgcccacg atgaactgag caacgagcac aaggcaacaa ccgagctgat gagccgaatc
       61 gtgactgact ctcaggaaat cagtcagacg aaggacagcc tgaatgtgaa ccagcaggaa
      121 cttgagaagc tgcgtgcgga ctacgaggca aaactcacac agctcgaagc aaatgagaag
      181 caggagctca agacggccca tgctgaggct caacgcatcg tcgacgacgt cagcagacgc
      241 atggacgacc taatgaagca gtaccaggac agcatcaaga accagcccca gatcgcccac
      301 gatgccaagc acgaggtcga gacaacgcgt gacgaactca ttcagaaggc ggagtcctac
      361 gaagagaagc ctccgtttac gccggtcgag gagatcctgc ctggtgacct cgtcatcatc
      421 acgtcggtca acaagcaggc catcgtcatc gacgtgatga aggacaagcg caaactcgtt
      481 cttcagtcgg gctccatgcg cacgacagtt cctgaggcac aagtgcgcaa gctttcggag
      541 cccgagctca actcatacca gaatcgcgcc atgcaataca gtggagcgcg ggaaccgacc
      601 ctgccattcg tccccatgaa gatcgacctg cacggaagcc gtgtcgatga ggcactcgag
      661 aaactcgaca agtacatcga cgtcgcctac ctgtcaggcc tttcattcgt ctacatctgc
      721 cacggcaagg gttcgggtat cctgcgaaag cagatccatg agttcctcaa gaccctgcca
      781 agtattgatc acttcagctt ttcgagccct gaagagggtg gcgatggggt gacaattgcc
      841 tacttcaagt agaaaggaac catttgaaaa acgaacagga actcgtcttc ttcgatctcg
      901 aaacaacagg tctcgaccag gcttccgatc agatcattga gattggtgca gtcaaggtac
      961 gcgggggaaa ggaagttggg cgatttgaac agttctgtcg cctgagagag ggcggccgcc
     1021 tgccggagtt catctccgcc ctgactggaa taaccactct cgacctcgaa ccggcggaat
     1081 cggtggacca agtggtggac cagttcctgg agttcgcggg tagtagccca ctgttggcac
     1141 acaattccaa cttcgacgaa gggttcctgc gcagggcacg tcatggccag ctctcgaatg
     1201 ccgtgtatga caccctggaa ttggcacgcg tattcttccc ggacctccag agccacagcc
     1261 tcgtcgctct cgtcgattcg ctggcgatca agaaagaaga agctcatcgt gctctcggcg
     1321 acagcctttc cctgtttcgc ttctaccgga aactgaagga caagatatca cagtctgctc
     1381 tgcctgattc catcgtgcgg cgaatccagt tcctctaccc ggtcatcgca gacctggggt
     1441 tgctggacaa cactgacacc agggtagccg tcgggccatc tcagatgccg ggcctggaga
     1501 cggtcttcac cagcctcgac tactctcgtc tgccccatct gaaggctctg agacctggca
     1561 tctccagtgc agcactctct ccagagatgg tattccttgc ttcgggacga gatgttctgc
     1621 tgcagtgtga gcctcagtcg ctccgagcac ggtatcttgc ggacgtccag gcgtcgagcc
     1681 gccccggcgc tgtgctgctt cgtgctcctg cagctatcga accgctcctg gaggacgcgc
     1741 gtcctggaga ccagcgtcca ctgtggatat cacaggagag cccgaacaac ctggtctgtc
     1801 tcatgcttct ggatgaagca agccgcagcc ctaccatgcg tgacgagttc ggctttgaac
     1861 tctccgctct tctcgtttat gaatgggaaa cgcgctcagt cttcatcccc gccatgcctc
     1921 tgttcctgaa gaagtcccgc cttatcaacc acatatcggc agtccattgc caaatcgagt
     1981 cgagctgtcc gtttcacgac gtgtgtcccg tccgcgagtc actggcgcgt gcgcgtatcg
     2041 ctcacctgca tgtgactgac ctcgcagagg caccgcacgt gctccgggaa ctcggcgaat
     2101 cgacgtccgt ccttgtatcc tcgcccgaga ctgagaaact catgagtgac gtactcgacg
     2161 tgcccgaggt gcaggtagca tcgatcacgc tgcgcatcat cgccggcatc gccggagaac
     2221 gacgtcaggt cccaggactc gaagtggtgg ccgacctggc cgcgcgcgcg cggactctcc
     2281 ttgacgaact gtatctgcgt gtcacgcatc aggagaacgc gggcgtccgt ggacgtatcg
     2341 caattggcga agcgttgtgg tctgcaggcg agttcgccgc tctcagatcg acggcggaag
     2401 cgctggtcga tgctttcatg aacgcaggag agtctctacc tgctgcgatg cgagcatcct
     2461 atctcatgca atgtcaggat ctggcctccg tgctggacaa cgccgacaac cctgcctacg
     2521 ccgtatggat ggaacgtcag ggtcaggaga tcacgctggc cagcaccagg acgatccttg
     2581 ccgcgcgagt ccgggcgtgc aatagcggcg cccagttctt tcttgccggg accagcgtgt
     2641 gcccggccgg tgaccagcga ttcctgccag agacattcgg cctcatggac ccggagcccg
     2701 ctgccgttgt cgtcgatggc gagcctgaag tccctcgcgt ccccactctt gtgacattgc
     2761 acctgccaaa gcccaatgaa tcaggattcg agaaggaagg tgcccggctc atcgcagaga
     2821 ccatgcgacg cttccccggc aagtcgatgg tcgcctcaaa ctccctcgag acgctgcgcc
     2881 gcatgcagtc atttctctcg cgggaagctg ggctgtcgga ctacagtctg ctgcttccaa
     2941 agaacgggca cactcgcaca caactactgg cgcaattctc gggtcaggac agggcaatcc
     3001 tgcttgtccc gttcgactac ctgagcttcg gcgatatcgt gtccgcaaag ttcctgttcg
     3061 tgaccaagct ccagttcccc aactcgttcc tgccaaccgt gacgcggctc aaacaggcgc
     3121 tcaaggagag aaggggcgat ccattccggc aatttgacct gccatatgcg ctgaccctgc
     3181 tgcagtacac actgcaccag atggaccctc gtacgctgcg gagtgtcttc atgctggaca
     3241 accgcagctt cggctcggcc tatgccgacc gtatccggac ggtccttccc acgccgtcgc
     3301 tcttcctgcc catggagaac caggagagcc ttttgcagca tcttgaccgg tggatgcgga
     3361 accagaccct ctgatggagc gagggaacac agttcgggaa cagcccggcg acgtcaggtg
     3421 ggcataggat tcgcggaggc tcgtgatgtg tgacacgctt gtggcacttg ctccaatgac
     3481 actgaacggg catacgatgc tggcgaagaa cagcgaccgc gagccaaacg aggctcaact
     3541 gctgacagtc atgccacgca ggacccatgg cgaggcgacg ctgcagacca cctatgtcga
     3601 ggtcgaccag gtccgcgaga ctaacgcctg tc
//
LOCUS       DATMZD010000190         2919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_146170,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000190 DATMZD010000000
VERSION     DATMZD010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146170"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1279
                     /locus_tag="VN478_03925"
     CDS             <1..1279
                     /locus_tag="VN478_03925"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HWQ21856.1"
                     /translation="VDTVIANGAECEPMLRCDQQLMAAHPELVIRGMRLVMQAVGAGR
                     GLIAVKAHYADAVTAMQAELDTQKDSDEAQRWPVSLYIIEKSVYPAGDEFCLVYEVTG
                     RLIPEAGLPLNVGCVVQNVGTLVNIARAVDEGTPVTHRYVTINGEVNSPRTVYAPLGM
                     SFDKVLELAGGYNRSESEIRVVVGGPMTGHLVSDLKAETITKTTGGILVLPADNEVVR
                     FMSRSLELWTRRGKASCDQCRDCTVLCPRHLLGHGFSPHEIMRAGAYGINVKNSVLTG
                     AVMCCECRLCEAYSCPLELSPMQFYKKLKRDLAAAGWKNDEHHRADLTPDDTREGRLV
                     PTERLVARLGVGTYHSWPVAWDESEVRADHVAISLKQHIGVPAQPVVAEGDTVSAGQL
                     IAKPPEGKLGAGIHASIAGRVTAVSDKAIVIEG"
     gene            complement(1375..2742)
                     /locus_tag="VN478_03930"
     CDS             complement(1375..2742)
                     /locus_tag="VN478_03930"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWQ21857.1"
                     /translation="MRVLVVGGNAGGASFATRLRRLRPDAEVVVFERGQYVSYANCGL
                     LYYVEGTIADRSALFVETADSLRSGHGIDVRLQSEVVGIDPSAHTITVHSTAGNGQSI
                     EHYDKLVLAMGAEPVLPRVPGIDAANVIPMWSIPHMDRIAALVKAGARSAVILGGGYV
                     GCVMAEALHKRGIAATIVEASAQTVSFLDPDIAAYAQQELERNDIAIRLGQRVERLAG
                     RGRGQRVTLTNGEDIDTDFVVVAVGVRPSSRIARDAGLAIGDSGGIAVSPSLRTSDAD
                     IYAIGDVIEVTHSVSGAETAIPLAGPAHQQARIAAANVAGVGKGWGEQYAGTQGSSIL
                     KLFDLSCGSTGANSAQLRRRNIPFDSILIHPLNHAGYYPDAFPIHLKLLYGPKGRVLG
                     AQAAGREGVDKRLDVIATAIYFGANVNDLDGLQLTYSPPFSSARDPVNVAGSVAQSRL
                     TSGKT"
BASE COUNT          569 a          881 c          911 g          558 t
ORIGIN      
        1 tgtcgacaca gtcattgcga acggagctga gtgcgaaccc atgttgcgat gcgaccagca
       61 gctcatggcg gctcatccgg aactagtcat ccgcgggatg cgtctggtga tgcaggcggt
      121 cggggcaggc cgtggactga tcgcggtcaa ggcgcattac gcggatgccg tcacagccat
      181 gcaggcagag ctggacaccc agaaggactc tgatgaagca cagagatggc cggtgagtct
      241 ctacatcatt gagaagagtg tgtatccagc gggcgacgaa ttctgcctgg tctatgaggt
      301 cacaggccgt ctcatccctg aggctggact tcccctgaat gtgggctgcg tcgtccagaa
      361 tgtcggcaca ctggtcaaca ttgctcgcgc ggtcgatgaa gggacgccgg ttacacaccg
      421 gtacgttacg atcaatggcg aggtcaactc gcccaggacc gtctacgcac ccctcggcat
      481 gtcctttgac aaggtgctcg agctggcagg tggctacaat cggagtgaaa gcgagatcag
      541 ggttgtcgtc ggcgggccga tgaccgggca cctcgtgtcc gatctgaagg cagaaacgat
      601 cacgaagacg acgggcggca tcctggtact ccctgccgac aacgaagtcg tccgattcat
      661 gtcgcgatct cttgagcttt ggacgagacg agggaaggcc agctgtgacc agtgtcgtga
      721 ttgcacggtc ctgtgtccgc gccacctgct cggacatgga ttcagccctc acgagatcat
      781 gcgtgcggga gcctatggca tcaacgtcaa gaacagtgtc ctgaccggcg ccgtcatgtg
      841 ctgcgaatgc cgtctctgtg aggcctattc ctgcccgctg gaactctctc cgatgcagtt
      901 ctacaagaag ttgaagcgcg atctggcagc cgcagggtgg aagaatgatg agcaccatcg
      961 tgccgatctg acgcccgatg acacccgcga aggacgcctc gtgccaactg agcgtcttgt
     1021 agcccgtctc ggtgtcggaa cgtaccactc gtggccggtc gcatgggacg agtccgaggt
     1081 ccgagctgac cacgttgcca tcagtctgaa gcagcacatc ggcgtgccgg cacagccggt
     1141 cgttgctgag ggcgacaccg tgagtgcggg ccagctcatt gccaagcctc cggaaggaaa
     1201 gctgggagct ggtatccatg catctatcgc cggcagggtt acggccgtca gcgacaaggc
     1261 aatcgtcatc gaaggttgat gactgcatag cagcgagaca actatcgaag aaccgcccaa
     1321 cgacctattg gcgtcgttgg gcggttcctg gttggaagag agcgtcgtgt ctgttcaggt
     1381 cttcccggat gtcagacggg actgcgctac tgagcctgcc acattgacgg gatctcgcgc
     1441 cgagctgaac ggaggcgagt aggtaagctg caggccgtcc aggtcgttca cgttggcccc
     1501 gaagtagatc gcggtcgcga tgacgtcgag gcgcttgtcg accccctcgc gccctgccgc
     1561 ttgagctccg agcacacggc ccttgggccc gtagagcagc ttcagatgga tggggaatgc
     1621 gtcagggtag taccccgcgt ggttgagcgg atggatgagg atggagtcga acgggatgtt
     1681 cctgcgccgc agctgtgccg aattggcgcc agtcgagcca cacgagaggt cgaagagctt
     1741 caggatggag ctgccctgcg ttccagcgta ctgttcgccc caacccttgc ccactccggc
     1801 aacgttggcc gccgcgatgc gggcctgctg atgggcgggg cctgcgagtg gaatggccgt
     1861 ctcggccccc gacacggagt gagtgacttc gatgacgtcg cctattgcgt agatatctgc
     1921 gtctgatgtg cgcaggctgg gcgacactgc aatccctccg gagtcgccga tcgcaagtcc
     1981 tgcgtcccgt gcgatacgcg acgatggccg gacaccgact gccacgacaa cgaagtctgt
     2041 gtcgatgtct tcgccgttcg taagggtaac cctctgtccg cggccacgac ccgccaggcg
     2101 ttcgacacgc tgccccaggc ggatggcgat atcattcctc tcgagttcct gttgagcata
     2161 tgccgcaatg tccggatcga ggaaggagac cgtctgggca gacgcctcga cgatcgtggc
     2221 ggcaatcccc cgcttgtgca gggcttccgc catcacgcag cccacgtagc ctccgccgag
     2281 gatgacagcg gaacgcgccc cagccttgac gagcgctgcg atcctgtcca tgtgagggat
     2341 cgaccacatg ggaatgacgt tggcggcgtc gatcccgggg acccttggta acacgggttc
     2401 tgcgcccatg gccagcacca gcttgtcgta gtgctctatg ctctgcccgt ttccagccgt
     2461 gctgtggacg gtgatcgtat gcgccgacgg atcgatgcca acgacctcgc tctggagacg
     2521 gacgtctatg ccatgccccg aacgaaggga gtcggctgtt tcgacaaaca gtgccgacct
     2581 gtcggcgatc gtgccctcca cgtagtacag aaggccacag ttggcgtacg agacatattg
     2641 cccacgctcg aagacgacga cttctgcatc agggcgcaga cgtcgaagac gcgttgcaaa
     2701 gcttgccccg cccgcattgc ccccgacgac cagcactctc atggcatcac atctcctggc
     2761 tgctgtctgt ctcctgctac tgctttggct tgtgggtacg ttccgtcccg atgccaaaga
     2821 agcccgtgag cccgaccacg aagagaacgc cggcagcgaa ccaccggaca aactcgtgtt
     2881 cgttcattcc cagcataatc aaaccccata cgactgcca
//
LOCUS       DATMZD010000191         2580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_36402,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000191 DATMZD010000000
VERSION     DATMZD010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36402"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="VN478_03935"
     CDS             <1..787
                     /locus_tag="VN478_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HWQ21858.1"
                     /translation="FSTDYFGVPAYLSQSGQLYNEATAMAFGKVYCFGPAFRAEKSKT
                     RRHLTEFWMVEPEMAYCDWSQNCDVQEQFVTYIVQQVLAERKEELAIIERDTVPLEKI
                     VAPFPRISYDEAVKLLQKAGSPIQWGEDFGGDEETLISNQFERPVFVHSYPKSFKAFY
                     MKTNPANPQTVLCADLLAPEGYGEIIGGGQREDDYELLLERVHAEGLSEADYQWYLDL
                     RKYGSVQHSGFGLGVERTVAWICKLPHVRETIPFPRLLEKIYP"
     gene            798..1823
                     /locus_tag="VN478_03940"
     CDS             798..1823
                     /locus_tag="VN478_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21859.1"
                     /translation="MTAVRGHASDSSDISVTEGLTSLRSKKPAPLYVFAPGDEYFVPQ
                     AKEAARSLVGEAFRAFDYAEFDGTDDNLDGIREFLGSESFGSGRKVLVINGLEDKGAR
                     RLATIAPLFLSGNTTSVMFADPAKVPLDLLKKALVVTNYAVPPTAVRSWIRKRFEGRA
                     VEDQAVHELMERTASSFFLMRSEIDRLLAYTNGPIAVADVRAVVPRCHTAAAADLVEA
                     ISLRKYDRAAMALHDLSDSGAPDTTVLYDVELGFMRLLNAKLVSKSNRPKADLRSWCL
                     RAQHQYLDDYTLNALLAAASHIRLFELEAMLERLEGIEYETKKGLTSSALAALEQLLS
                     AVVLTKQ"
     gene            complement(1810..2079)
                     /gene="rpsT"
                     /locus_tag="VN478_03945"
     CDS             complement(1810..2079)
                     /gene="rpsT"
                     /locus_tag="VN478_03945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HWQ21860.1"
                     /translation="MPIKRASVKDVRKTKTRTAYNTEHTAKTKLALDMARKSDYAPEK
                     VVDAVKQLDKLAQKGIVHKNTAARKKSRLMKKANAAKAATKTTAS"
BASE COUNT          523 a          727 c          768 g          562 t
ORIGIN      
        1 gttttcgact gactacttcg gcgttcccgc atacctgtcc caaagcgggc agctatacaa
       61 cgaagcgacg gccatggcgt ttggcaaggt ctactgcttc ggtcctgcgt ttcgtgccga
      121 gaaatccaag acccggcggc atctgaccga attctggatg gtcgagcccg agatggcgta
      181 ctgcgactgg tctcagaact gcgatgtcca ggagcagttc gtcacctaca tcgtgcagca
      241 ggtcctggcg gagcgcaaag aggagcttgc catcatcgaa cgggacactg taccgctcga
      301 gaagatcgtg gcgccgttcc cccgcatctc gtacgatgag gctgtgaagc ttctccagaa
      361 agccggttcg cccatacagt ggggcgagga ctttggtggc gacgaagaga cgcttatctc
      421 gaaccagttc gagcgtcccg ttttcgtgca cagctacccc aagtcgttca aggcgttcta
      481 catgaagaca aatcccgcca atccacagac ggtcctgtgt gccgaccttc tggctccaga
      541 gggatatggg gagatcatcg gaggtgggca gcgcgaagat gattatgagc tgctgctcga
      601 acgggttcat gctgagggac tctctgaagc cgactaccag tggtaccttg accttcgcaa
      661 gtacggatcg gttcagcaca gtggatttgg tcttggtgtc gagaggacgg tcgcgtggat
      721 ctgcaagctg ccgcatgtcc gggagacgat cccgttccct cgcctgctcg agaagatcta
      781 cccctagacc ggcagcaatg acggcagtca gagggcatgc ctcagactcc agcgacatct
      841 cggtaaccga gggtctcaca agtctcagga gcaagaaacc agccccactc tacgtttttg
      901 ctcctggaga cgaatacttc gtgccgcagg cgaaagaagc ggctcgctcc ttggtgggag
      961 aggcttttcg cgctttcgac tatgccgagt tcgacgggac ggacgacaac ctcgacggca
     1021 tccgggagtt cctcggcagc gagagcttcg gatccggacg gaaggtgctc gtcatcaacg
     1081 gcctcgagga caagggagcc aggcgtctgg ccacgatcgc ccccctgttt ctgtccggca
     1141 acacgacgag cgtcatgttt gcggacccgg ccaaggtgcc cctggacctg ttgaagaagg
     1201 cgcttgtggt cacgaactac gccgtacctc cgaccgcggt gcgttcctgg atcaggaaga
     1261 ggttcgaggg acgcgcagtc gaggaccagg ctgtgcacga gctgatggaa cggacagcga
     1321 gcagcttctt cctcatgcgc agcgagatcg acagactcct cgcgtacacc aacggaccga
     1381 tagctgtcgc cgatgttcgc gcggtcgttc ccaggtgtca cacggccgct gcggctgatc
     1441 tcgtcgaggc gatctcgctc aggaagtatg atcgtgcagc catggcgctc catgacctgt
     1501 cggattcggg ggccccggac acgaccgttc tatatgatgt ggaactcggg ttcatgcgcc
     1561 tgctgaacgc gaagctcgtc tcgaagtcga accgccccaa ggcggacctc aggagttggt
     1621 gtctacgcgc gcagcatcag tacctggacg actacacctt gaatgccctt ctggcagccg
     1681 caagccatat cagattgttc gagcttgagg ccatgctgga gcgtctcgag ggaatcgagt
     1741 acgaaacaaa aaaagggctc accagcagtg cgctggcagc ccttgaacag ttgctttcgg
     1801 cagtagttct tacgaagcag tagtcttggt cgcagccttg gcagcattcg ccttcttcat
     1861 caggcgcgac ttcttgcgag cagctgtatt cttgtggacg atgcccttct gagcgagctt
     1921 gtcgagctgc tttactgcat cgacgacttt ctcgggggca tagtcggact tgcgagccat
     1981 atccaaggca agcttcgtct tggctgtatg ctcggtgttg tatgccgtcc gtgtcttggt
     2041 cttgcggacg tccttaacag aagctctctt gattggcatg aaactctcct ttgtctcttc
     2101 ggaacttatc atgtttactg tacccgtttt tcgcttgatg tcaatgattg ttcgcttcgc
     2161 cggccgccag accgggccct ccaaaccaca catcttgacc attgagtcgg tagggagtac
     2221 agtaccgacg ggaagccgcg acgaagggct ccgattggag gggcgcacga aagaaagcag
     2281 atgcgccggc tgttcctccg gcgacttggt tgatgttgtc cgcactgaac tgatcctgtg
     2341 tggccaccag gctatccacg ggatttcgcg gacaggcgaa gtggcgctct gcagggtggg
     2401 agatattgcc ttgacttcat gtggtcctgt tatataattc acatggcacc gtggaatcag
     2461 cagcggaatc cgggttaccg catctgcact gatgcaagca tccttcaacg cgtcacgcgc
     2521 gtgtgggaag aagaggaggc actactttgg agtgtctttc acctgcgaag atcgctgaac
//
LOCUS       DATMZD010000192         3090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_25425,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000192 DATMZD010000000
VERSION     DATMZD010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25425"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VN478_03950"
     CDS             complement(<1..234)
                     /locus_tag="VN478_03950"
                     /inference="COORDINATES: protein motif:HMM:NF013030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HWQ21861.1"
                     /translation="MSPQQRIRASVSILGADFADLGRALERIRMSGADSIHLDIMDGR
                     FVPNISFGWSIASDVVRASGLPCCAHLMVEHPED"
     gene            complement(231..941)
                     /locus_tag="VN478_03955"
     CDS             complement(231..941)
                     /locus_tag="VN478_03955"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HWQ21862.1"
                     /translation="MRVMMRIWQRCLTIVVLACVLSVTAGCSSRTAGQNMVQAPVLVG
                     VSLREAEARAAKAGVLVEVQSSEDSSTMPVDFVLRQDPEPQTMVRKGRVISVVTSSGP
                     VSLTLPDFVGGKFETAQAFISANQLVLGDLVEKIDASPVGTVLSQDPVASGDVTRGSV
                     VTLTISKGTMAVMPDLVGLSLTEARKVLTPLGFSVSKVIVSAQTTQPAQLVLMQEPAV
                     GDAIEKGAWIELTVSKVP"
     gene            1175..2491
                     /locus_tag="VN478_03960"
     CDS             1175..2491
                     /locus_tag="VN478_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018963356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HWQ21863.1"
                     /translation="MKKRVLIMGAAGRDFHNFNVVFRDNPDYEVVAFTATQIPGIDDK
                     KYPAALAGKLYPAGIPIYPESDLDRLITELNVDEVVFAYSDQPHVKVMNKASQVLADG
                     ADFSLLGPKSTEIKSSKPVISICAVRTGSGKSQTSRAVVRALRAAGKKVVSIRHPMPY
                     GDLAAQACERFATYADLDKYKCTIEEREEYEPHIDMGAVIYAGVDYEMIVREAEKEAD
                     VIIWDGGNNDFSFYVPDLKITVADPLRAGNELTYYPGETNFRQADVIVVNKVDSATPA
                     QLATVRESMYRVNPKAIMIEAASPVFVKDPDMIRGKRVLVIEDGPTLTHGEMTYGAGY
                     VAAMKYGAAEIVDPHPYAVGSIKATFEKYTQKMSILPAMGYSDEQIEDLRQTIEATPC
                     DVVVMGTPIDLLRVLKLTKPSVRVTYELQEIGTPTIVDVLKKFGFI"
     gene            2504..>3090
                     /locus_tag="VN478_03965"
     CDS             2504..>3090
                     /locus_tag="VN478_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012544822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HWQ21864.1"
                     /translation="MATKLKGRDLLCLKDFSKEEVLEIVSFGDKLKMDSMVGKQEHYL
                     EGKSVAMIFQKASTRTRVSFEVAAFELGAHALYLNANDMQLHRGETISDTAHVLSRYV
                     AGIVARVFAHQDLVDLAANSDSPIINALSDLSHPCQILGDLMTIREKKGTLKGLRLAY
                     VGDGNNVANSLLLGCARVGMNIAIASPKGFEPKEE"
BASE COUNT          722 a          960 c          838 g          570 t
ORIGIN      
        1 gtcttcgggg tgttcgacca tcaggtgcgc gcagcacggg agaccagagg ccctcacgac
       61 atccgacgct atcgaccagc cgaatgagat gttcgggaca aagcggccgt ccatgatatc
      121 caggtgaatg ctgtcggctc ccgacatccg gatccgttcg agggcccgtc cgagatctgc
      181 gaagtccgcc cccagaatcg agacagacgc ccggatacgc tgttgtggac tcatggaacc
      241 ttcgatacgg tcaactcgat ccaggcaccc ttctcgatag cgtcgccgac agcaggctcc
      301 tgcatcagca cgagctgggc aggctgggtc gtctgagcag aaacgatgac cttgcttact
      361 gagaagccca atggggtgag caccttcctc gcctcagtca gagacaaccc aaccagatca
      421 ggcatgacgg ccatcgtccc cttgctgatg gtgagcgtca caacagatcc cctggtaacg
      481 tcgccgctgg cgacaggatc ctgagacagg actgtcccca ctggactcgc atcgatcttc
      541 tcgacaaggt cacccaggac aagctgattt gcactgatga aggcctgagc cgtctcgaac
      601 ttgccaccga cgaagtcagg aagcgtcagg gagacaggtc ccgaactggt cacgacgctg
      661 atgacgcggc cctttcgcac catggtctga ggttcgggat cctgtctcag gacaaagtcc
      721 acaggcatcg tgctcgaatc ctctgaagac tgcacttcaa caagaacccc cgcctttgcg
      781 gctctcgcct cggcttcgcg gaggctcacg cctacaagga ccggagcctg aaccatattc
      841 tgaccggcgg tgcgggacga acaaccggca gtcaccgaca gcacgcatgc aagcacaacg
      901 attgtcagac atctttgcca gattcgcatc atcactctca tgtcacctgc agaccgacgg
      961 agtcggaata cctgccatat cacgccgggc acaccagccc gaactcatgc atcacgcata
     1021 gccagctcac aaacactctt ccgaaccatt atacggccag gcgccaatag tcaaccgcaa
     1081 acaggcgcaa gtcatttgac atcgaggccc aaggctatac aatcagagtg ccccaaagcg
     1141 ggcattggac atcattctct gtagaggagg atttatgaaa aagagagtct tgatcatggg
     1201 agctgcaggc cgggacttcc acaatttcaa cgtggtattc cgggataacc cggactacga
     1261 agtggtggca ttcacggcaa ctcagatccc cggcatcgac gacaagaagt accctgccgc
     1321 cctggccggc aagctctatc cagctggtat tccaatctac ccagagtccg atctcgacag
     1381 gctcatcaca gagctcaatg tcgatgaagt agtattcgcg tactccgatc agccacatgt
     1441 gaaggtcatg aacaaagcct cacaggtcct ggccgacggt gccgacttct ccctgctcgg
     1501 cccgaagagc accgagatca agtccagcaa gccggtcatc tccatctgcg cggtccgcac
     1561 tggatcgggc aagagtcaga ccagccgtgc cgtcgttcgc gctctgcgcg cagccggcaa
     1621 gaaggtcgtg tccatccggc acccgatgcc ctatggcgac ctggctgccc aggcgtgcga
     1681 acgctttgca acctacgccg acctcgacaa gtacaagtgc acgattgagg agcgagagga
     1741 gtacgagcct cacatcgaca tgggcgctgt catctacgcc ggcgtcgact acgagatgat
     1801 cgttcgcgaa gcagagaagg aagcagacgt catcatctgg gacggcggca acaacgattt
     1861 ctcgttctac gttccggacc tcaagatcac ggtcgccgat cctctccgcg cagggaacga
     1921 actcacctac tatcctggcg agaccaactt ccggcaggca gacgtcatcg tagtcaacaa
     1981 ggttgactcg gccaccccgg ctcagttggc gacagtccgc gaaagcatgt acagagtgaa
     2041 ccccaaggcc atcatgatcg aagcggcctc tcccgtgttc gtcaaggatc cggacatgat
     2101 ccgcggcaag cgcgttctcg tcatcgagga cggccccacg ctcacccacg gcgagatgac
     2161 gtacggcgca ggatacgttg cggccatgaa gtacggcgcc gccgagatcg tcgaccccca
     2221 tccctatgcc gtcggatcca tcaaggcaac gttcgagaag tacactcaga agatgagcat
     2281 cctgccggcc atgggctaca gcgacgagca gatcgaagac ctgcgtcaga caatcgaagc
     2341 gaccccgtgc gacgtcgtcg tcatgggcac acctatagac ctgttgcgcg tcctcaagct
     2401 caccaagccg tcggttcgtg tcacctatga actccaggag attggcacgc ctaccatcgt
     2461 agatgtcctg aagaagttcg gtttcatcta gtctgggggg attatggcca caaaactcaa
     2521 gggaagagac ctgctctgcc tgaaggactt ctcgaaggaa gaagtcctcg agatcgtctc
     2581 gtttggtgac aagctgaaga tggattccat ggtggggaag caggagcact acctggaagg
     2641 caagtccgtc gccatgatct tccagaaggc ctcgacccgt accagagtat cgttcgaagt
     2701 cgctgcattt gagcttggag ctcacgcact gtatctcaat gcgaacgaca tgcagctgca
     2761 ccgcggcgag accatctcag atacagcaca cgtcctctcg cgctacgttg ccggtatcgt
     2821 tgcacgggtg tttgcgcatc aggacctcgt cgaccttgcc gccaacagcg attcgcccat
     2881 catcaacgct ctgtccgacc tgagccatcc ctgccagatc cttggggacc tcatgacgat
     2941 ccgcgagaag aagggaacgc tcaagggtct cagactcgcc tacgtcggcg acggcaacaa
     3001 cgtcgccaat tccctgttgc ttggatgcgc acgtgtcggc atgaacatcg ccatcgcttc
     3061 gcccaaggga ttcgagccca aggaagagat
//
LOCUS       DATMZD010000193         2017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_75358,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000193 DATMZD010000000
VERSION     DATMZD010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75358"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="VN478_03970"
     CDS             complement(<1..740)
                     /locus_tag="VN478_03970"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWQ21865.1"
                     /translation="MAVYVGTVPPVGWEKVWDEAARRAGYTGPRPQVITDTAELARHG
                     DDMGALVTKSVTPELLAAAPHLKLVQVPFAGVDSVDLATIRHSGVLLANSHGNREVTA
                     EHAVALMFALTRRIIQYDRDLRKGIWHGFATMDDPVIGLFGKTVGIVGLGNIGMHILR
                     ICRNMNMKVLGLKHSPGTTCSDLVDRAYGSDERMEFLRACDFVIDVLPLTAETEGFFG
                     AAEFEAMRGHWFINIGRGRTVDEHALYVA"
     gene            complement(834..1682)
                     /locus_tag="VN478_03975"
     CDS             complement(834..1682)
                     /locus_tag="VN478_03975"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HWQ21866.1"
                     /translation="MDELTTINSATATAMAADMKVALLATVNGDGQPHVTLITTVQAR
                     DPYTVMWGQFTEGLSKQNVHANPKTAFLVMSLTRKIWRGRALWKEERKEGSEYEMYNN
                     KPMWRYNTYFGIHTVHYMQLEGITEGQPLPMGQVIRGGLMTRLARTSLATGNPDRILS
                     PWAQGMLGKLDSLKFVSYVGADGYPVIIPCIQAQAADSRRIAISALPYGGELRAIPDG
                     TKVALFGMTLDMEDVLLRGVWTGFRRHGVAELATIDIDWVYNSMPPVMGQIYPPIPLE
                     AVTRFE"
     gene            complement(1675..>2017)
                     /locus_tag="VN478_03980"
     CDS             complement(1675..>2017)
                     /locus_tag="VN478_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21867.1"
                     /translation="GATAADLNIRVLGAFIAENLADVASRWDLCAFNNLARDKYARLG
                     RDWLYAGTPLMSADEVRRLVDVARSSGVNPGIVHWSGRTKLDDPLVVEDHSADPGPSS
                     PGGFQHGGCNG"
BASE COUNT          352 a          645 c          632 g          388 t
ORIGIN      
        1 gcaacataca gcgcgtgttc gtcgacagtg cgcccgcggc cgatgttgat gaaccagtga
       61 ccacgcatcg cctcgaattc tgcagccccg aagaagcctt ccgtctctgc ggtgagcggc
      121 aggacgtcga tgacgaagtc gcaggctcgc agaaactcca tcctctcatc cgagccgtac
      181 gcccggtcga ccaggtcgct gcacgtcgtc cccggactgt gcttcaggcc caggaccttc
      241 atgttcatgt tgcggcatat cctgaggatg tgcatgccga tgttgcccag acccacgatg
      301 ccgaccgtct tgccgaacag gcctatgacc ggatcgtcca tggtggcaaa accgtgccag
      361 atgcccttgc gaaggtcgcg gtcatactgg atgatgcggc gagtgagcgc aaacatgagg
      421 gccaccgcat gctcggcagt cacctcacgg ttgccgtgcg agttggcaag taggaccccg
      481 gaatgcctga tcgtcgccag gtcgacgctg tcgaccccgg cgaacggtac ctggaccagc
      541 ttgagatgag gcgcagctgc cagcagctcc ggcgtcacgg acttggtgac cagtgcgccc
      601 atgtcgtccc cgtgcctggc gagctcagcg gtatcggtaa tgacctgtgg acgcggtccg
      661 gtgtagcctg cccttcgtgc cgcttcgtcc cataccttct cccacccgac aggtgggact
      721 gttccgacgt acactgccat agtggcacct cctgtgcctc agattcgtgc ttctgtccta
      781 ttgtaccctt gtttcgacag acacgtcgag agcagcgcag aatgccgtca agcctactcg
      841 aatcgagtga cggcctccag cgggatcggc gggtagatct gtcccatgac cggcggcatg
      901 ctgttgtaca cccagtcgat gtcgatggtg gccagttcgg ccacgccatg gcgacggaaa
      961 ccggtccaga cgccccgcag gaggacgtcc tccatgtcca gggtcatccc gaagagcgcg
     1021 acctttgtac cgtcggggat ggctcggagc tcgcccccgt aggggagagc tgagatggcg
     1081 atgcgcctgc tgtcggccgc ctgcgcctgg atacagggga tgatcacagg atacccgtcc
     1141 gccccgacgt agctcacgaa cttgagtgag tccagcttgc ccagcatccc ctgggcccac
     1201 ggcgagagga tacggtcagg gttgcccgtc gcgagagagg tgcgcgccag ccgtgtcatc
     1261 agtccacccc ggatgacctg gcccatagga agcggctgcc cctcggtgat accctcgagc
     1321 tgcatgtagt ggaccgtatg gatgccgaaa tacgtgttgt accgccacat gggcttgttg
     1381 ttgtacatct catactccga cccttccttg cgctcctcct tccacagggc acgcccgcgc
     1441 cagatcttcc tggtcagcga cattacgagg aatgccgtct tcgggttggc atggacattc
     1501 tgcttgctga ggccctcggt gaactgtccc cacatgacgg tgtacgggtc gcgagcctgg
     1561 acggtcgtga tgagcgtgac gtgcggctgg ccgtcgccgt tcaccgtggc cagcagggcc
     1621 actttcatgt cggcggccat agcggtcgca gtcgcgctgt tgatcgtcgt cagttcatcc
     1681 attgcaccct ccgtgctgga agccgccggg gctggacggt cccggatcgg cagaatggtc
     1741 ttcgacgaca agcgggtcgt ccagtttggt gcgcccgctc cagtgaacga tgcccgggtt
     1801 cacgccggac gagcgggcga cgtcgaccag gcgacggacc tcatcggctg acatcagcgg
     1861 cgtgccggcg tacagccagt cgcggccgag gcgggcatac ttgtcccgcg ccaggttatt
     1921 gaaggcgcac aagtcccaac gggacgcgac gtctgcaagg ttctctgcga taaacgctcc
     1981 caggacacgg atgttgaggt cggcggcagt ggcgccc
//
LOCUS       DATMZD010000194         7600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_25490,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000194 DATMZD010000000
VERSION     DATMZD010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25490"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="VN478_03985"
     CDS             complement(<1..854)
                     /locus_tag="VN478_03985"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWQ21868.1"
                     /translation="MQHEDAALLHVRVMSSLGGWWKQTGDALYLRNGVIKRVGSTADV
                     QAMASGGSAHVLDFAGTPEATCYPAFMDAHLHLDLYGFSLLHVNLNGETSLGGALGRI
                     RAAGRPDNGAWVRGDCWDDELWHDGPHRRQLDDLYKDSPVVLNRKDYHSLWLNTAALK
                     AVDLWDSRPFGPDLVPCDADGPIGIVHDAAQDWVLSRIPAPRVDERFAALHSAVTSLL
                     SMGFASCCSMDYGILPELEALIPAMSDEPRIRIWQAVGLDNLDAAVSLGLRSGFGSDF
                     LRLGGIKI"
     gene            complement(968..1621)
                     /locus_tag="VN478_03990"
     CDS             complement(968..1621)
                     /locus_tag="VN478_03990"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HWQ21869.1"
                     /translation="MFDLYLFDLDGTLVDTRRDIAEAVNHVLQLHTRPVADLATVISW
                     VGDGLDDMMARAFQTDEHEFISELVDEFHSYYMDHCTDSSTVYEGCMDTLIALQKQGA
                     HLSALTNKPQDLSVEILEHLEILQLFDRVVGPSDAALRKPNPSNLLSLIRDVGTGKER
                     TLMVGDSRNDILVARNAGVASCGCTFGYIGRDALLELGPTYLIDTWPELLALPGPTR"
     gene            1784..2884
                     /locus_tag="VN478_03995"
     CDS             1784..2884
                     /locus_tag="VN478_03995"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWQ21870.1"
                     /translation="METRLIEDGPGFAQLEPFWNQLVVNSIANTCHSTFEWLFTWWKY
                     FGTDRHLLLVVAIEDGVPVGLAPLYAGSGTTEPRDLHFLGQGLSDYADFIAPRGRLDV
                     LEALIAAVLDLRSSWDGMDLEEMPAQSPTRERLDHAVKVRDVAAVWQTTVQCPYLPIE
                     TGWEAFYATMGKGFQHEVRNKLNRWNNRSTGRVEGMELRYVDRREVDSTFVDEVVALS
                     DKRRSVDSHRSPFLNHPDQEFLREVLPLMGRRNQLRVAELRSGSALLAFMLAYYWEGV
                     VYTWNTQYDPAYREYSLGRIVLVRFAEQSFREGCHELDFMRGEEPYKFQWTSLSRTNL
                     ALRTEPSDQAAAEAQEPGAGCALSSTERERST"
     gene            2886..4526
                     /locus_tag="VN478_04000"
     CDS             2886..4526
                     /locus_tag="VN478_04000"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF011442.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HWQ21871.1"
                     /translation="MRQKVIVTGHKNPDTDSICSALGYAFYKNRTDPARVYVAVRGGD
                     LNDETRFVLERFGFRSPPLLRSLMPQVQDIPRKRLVTVAPSTRVGKAAILMKENHIHS
                     LPVVDDALAVRGVVDAVDIATAYADQLEHADILPYAVELDTLVRQLAARIIVQSGGPV
                     ELKGQLLVVTGSVGRLAPRSEDVVIIISDGMPSEDVLAACPVASTLIITGGAISSQTE
                     AHWPAHLSLVLETDMTVTQALRALWSSIPVSAFMEGTVPLLGMTDTLERAKKAVLDSS
                     ARCAVVLDSLHHPVNIVTRSDLIRFARKKVVLVDHNETLQAVDGVEEADILEIIDHHR
                     VGDISTFRPIYFHNEPVGSTCTIVAELCTENGVFMPKSVAGLLMSGILSDTMNLNLST
                     TTPKDVRAVRRLAATIGIDAEVYGLELLQNGSVMFKGLPAADVLMRDFKEFDLFDTRI
                     GVSQAVVFSFDRIREREPEFKQAMQDMRTRMGLAMVAFLATDPLSRTSYLIVAGPSEA
                     FEEAFDVQMEDGHAVLDGILSRKKDFIPPVAEVLSRYA"
     gene            4618..5334
                     /locus_tag="VN478_04005"
     CDS             4618..5334
                     /locus_tag="VN478_04005"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HWQ21872.1"
                     /translation="MLNKQSHMPLYVQLKDELAGRIKHGAMKVGSQIQTESELMKQYG
                     VGRATVREAVALLVSEGYLVKRQGIGTFVCNLQPSMGFEPLISLTASLRARGVNPRNV
                     VTISERFVPGAELLAQLRWSTPETCTHVRRIRYAESAPIAVEDSWFSHEFQDVGEKYD
                     LTGSLSRIIIEDLKVDIARIDQVIVPRLPSSEEKSLLEFQGDARVLQMDRWIYIVGRE
                     TPFYTLRFIVPTDIYYSLGL"
     gene            5442..7163
                     /locus_tag="VN478_04010"
     CDS             5442..7163
                     /locus_tag="VN478_04010"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWQ21873.1"
                     /translation="MGRRSQRIQQRLQQQVEPGISCQEGHDSIILEGSVDNWAQVVRA
                     GKLAAHEGYKGVVNRLAVRGLTLPAPRSPKLKDTALDGRHVDVLIVGGGVTGCALARE
                     LTRKKISVLLVEKEDDLATHASSRNNGMIHPGIEPHPGTRKAHFNVRGNAMYDTVAAE
                     LDVPFARCGSLILYQERWERFLGPLFMARARQNGVPGVRLISKSEALSLEPHLTREFS
                     AALHMPSTGIISPYLLTVAYAENAVRNGAAISLETMVVDMKVREGRITAVRTNRGTLY
                     PRVVVNAAGLYADTVADMAGDQFFTIHPRKGEIIILDRKTGHLISHVVAKPRLLQVKS
                     DTKGGGINRTIDGNILVGPNALEQPFREDYSTDRDRIELMLRRYLPLVPELSSTDLIT
                     YFASTRPATYEEDFVVERSQYVRNLVHAAGIQSPGLASAPAIAAEVARMTADVLGEAI
                     EVADNPTFDGRRHGIPDPERMTWEERDALIHRRSDYGVIVCRCEGISKGQVVDALRSP
                     IPVASLDAVKRRVRAGMGRCQGGFCTPQVLGIIGEQNGGQLEAITKKGGDSFILATET
                     KSAQRAP"
     gene            7160..>7600
                     /locus_tag="VN478_04015"
     CDS             7160..>7600
                     /locus_tag="VN478_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWQ21874.1"
                     /translation="MMLRSVDVAVLGAGPAGMAAALAAAQGGANTVLVERENRLGGIL
                     KQCVHDGFGLLRFGEKLSGPEYAGRFVHMVREAGLPVLTSTFVAEAHRDGEDNILMLV
                     DRSGVTELHAKAVVLATGCRERSARQVMIQGTRPAGVMTAGAAQY"
BASE COUNT         1487 a         2202 c         2455 g         1456 t
ORIGIN      
        1 aagatcttga tgccgccgag tcggagaaag tccgagccga acccggaccg aaggcccaga
       61 gagacggcgg cgtcaaggtt gtcgaggccg acggcctgcc agatccggat acggggttcg
      121 tcgctcatgg cggggatcag cgcctccagt tcggggagga tgccatagtc catgctgcag
      181 cacgaggcaa accccatgga cagcaggctg gtgacggcgc tgtggagcgc ggcgaaccgt
      241 tcatcgaccc ggggcgcggg aatgcgcgag aggacccagt cctgagctgc gtcatggacg
      301 atgccgatgg ggccgtctgc atcacacgga accaggtcag gtccgaatgg gcgggaatcc
      361 cacaggtcaa ctgccttgag agcggccgta ttgagccata gcgagtgata gtccttgcgg
      421 ttgaggacca ctggcgagtc tttgtagagg tcgtcgagct gacgccgatg tggaccgtca
      481 tgccacagtt catcgtccca gcaatcgcct cgaacccagg cgccgttgtc cggtctcccg
      541 gccgcgcgga tgcgtccgag ggcgccgccc agtgatgtct cgccgttgag gttcacatgc
      601 agcagcgaga atccgtagag gtcgaggtgc aggtgggcat ccatgaaggc cggatagcag
      661 gttgcctcag gcgtaccggc gaagtcgagg acatgtgcac ttccgccgga tgccatcgcc
      721 tggacgtccg cagtgcttcc aacgcgcttg atgactccat ttcgcaggta cagggcatcg
      781 cctgtctgct tccaccatcc gccaagcgac gacatgaccc tgacgtggag tagtgctgca
      841 tcttcatgct gcatgatcac cttcctccct cgtccgcaca cggacgctca tcatcacaag
      901 aaccccgcag ccccgggttg catggtccgg aaccgacggg gtcattgtag atcggcaggc
      961 gcagccttca acgggtcggg cctggcagcg caagaagctc aggccacgtg tcgatgagat
     1021 aggtaggccc gagttcaagc agggcatcgc gcccgatgta gccgaacgtg cagccgcacg
     1081 aggctactcc ggcattgcga gccacaagga tgtcgttccg tgagtcccct accatgagtg
     1141 tccgctcctt gcctgtacca acgtctcgta tcagggacag gagatttgac gggtttggct
     1201 tgcgcaaggc ggcgtcgctg ggaccgacaa cacggtcgaa gagttgcagg atctcaaggt
     1261 gctcgaggat ctcgaccgag aggtcctgcg gcttgttcgt cagtgccgac aagtgagctc
     1321 cctgtttctg cagcgctatg agtgtgtcca tgcagccctc atagacagtt gaagagtcgg
     1381 tgcagtggtc catgtagtac gagtggaatt cgtccacgag ttcactgatg aactcgtgtt
     1441 catcggtctg gaaggctctt gccatcatgt catccagtcc gtctcccacc cacgagataa
     1501 cggtagcgag gtcggcaaca ggcctggtgt gcagctggag cacgtggttg actgcctcag
     1561 caatgtcacg acgtgtgtcg acaagcgttc catcgaggtc aaacaagtag agatcgaaca
     1621 caggcgtccc cctggcagtg gtgtggtgcg aacagttctc atgtatactg tactgatgca
     1681 cgggcacaag tctaccctgg caactgtgaa ccagcaccaa tgctggcggc agcgtgatgg
     1741 ttgctccaag gatgacatcc caggattgag aggaaggact tccgtggaaa cgaggctcat
     1801 cgaagacggc ccggggtttg cgcagctgga gccattctgg aaccagctag tagtgaacag
     1861 catcgcgaat acgtgccatt cgacgttcga atggctgttc acctggtgga agtactttgg
     1921 cacagacagg cacctcctgc tggtagtggc cattgaggac ggtgtgcctg tgggccttgc
     1981 gcccctgtat gcgggcagtg ggaccacgga gcccagagac ctgcacttcc tggggcaggg
     2041 gctttcggac tacgctgact tcattgctcc ccggggccgc ctggacgtgc ttgaggctct
     2101 cattgcggcc gtactggacc ttcgctcctc atgggatggg atggacctgg aggagatgcc
     2161 ggcccagtcg cccaccaggg agcgtctcga ccatgccgtc aaggtcaggg acgtcgctgc
     2221 tgtgtggcag acgaccgtgc agtgcccata tctgcccatc gagactggct gggaggcgtt
     2281 ctacgccacc atgggcaagg gattccagca tgaggtacgg aacaagctga atcggtggaa
     2341 caaccggagc acaggcagag tagaaggtat ggaactgcgt tacgtcgatc gcagagaagt
     2401 agacagcaca tttgtggatg aggttgtcgc gctgtcggac aagcgccggt cggtggacag
     2461 ccatcggagc ccgttcctca accatcccga tcaggagttc cttcgcgaag tgcttccgct
     2521 catgggtcgg cgaaaccagc ttcgcgtggc agaactgcga agcggaagcg ccctgctggc
     2581 attcatgctc gcctactact gggagggagt cgtctacacg tggaatactc agtacgatcc
     2641 cgcctatcga gagtactcac tggggcgcat tgtcctggtg cggtttgcgg agcagtcatt
     2701 tcgcgagggc tgccacgagt tggatttcat gcggggtgag gaaccgtaca agttccagtg
     2761 gaccagcctg tccaggacga atctggctct ccgcaccgag ccgtcggacc aggcggccgc
     2821 ggaggcgcag gagcccggag cagggtgtgc gttgtcgtcg acggagcgtg aaaggagcac
     2881 ctgatatgcg gcaaaaggtc atcgttaccg gacacaagaa tcccgacaca gactccatct
     2941 gctctgcgct tggctatgcg ttctacaaga accgcacgga cccagcccgt gtctacgtgg
     3001 cggttcgggg aggagacctc aacgacgaaa cccgcttcgt tctggaacgc ttcgggtttc
     3061 gctcgcctcc actgctgaga tcgctgatgc cacaggtcca ggacattccg cggaagcgcc
     3121 tcgttacagt cgctccttcg actcgcgtcg gaaaggccgc gattctcatg aaggagaacc
     3181 atatccactc gctgccggtc gtcgacgatg cgcttgccgt tcggggtgtc gtggacgccg
     3241 tcgacatcgc tactgcctac gccgaccagc tggagcatgc cgacatcctg ccgtatgcgg
     3301 tggaactcga caccctggtt cgtcagttgg ccgccaggat cattgtgcag agcggtggcc
     3361 cggtggagct caaggggcag ctgctcgtcg tcacaggctc ggttgggcgt ttggcgccac
     3421 ggtctgagga cgtcgtgatc atcatctctg atgggatgcc gtccgaggac gtcctggcgg
     3481 cgtgcccggt cgcctcgacg ctcatcatca cgggaggtgc catttcctcc cagacagaag
     3541 cgcactggcc ggctcacctg agcctcgttc tcgaaacgga catgaccgtg acgcaagcgc
     3601 tgcgcgccct gtggtcatcg atcccagtgt cggcgttcat ggaaggcact gtgcccctgc
     3661 tcggcatgac ggacacgctg gagagggcaa agaaggcggt cctcgactcg tcggcccgct
     3721 gcgcagtggt cctcgactcc ttgcatcatc cggtgaatat cgtgacgcgt tcggatctca
     3781 tccggtttgc gcgcaagaag gtcgtcctcg tggatcacaa cgagacgctg caggccgtcg
     3841 atggcgtgga ggaggctgac atcctggaga tcatcgacca ccaccgcgtc ggcgacatct
     3901 caacctttcg acccatctac ttccacaacg agccggtggg gagcacctgc accatcgtcg
     3961 ctgaactgtg tactgagaac ggtgtgttca tgccgaagtc ggtcgctggg ctcctgatgt
     4021 cgggtatcct gtcggacacg atgaatctca acctgtccac gactactccg aaggacgttc
     4081 gggccgtacg tcggctcgcg gcaaccatcg gcatcgacgc ggaagtctac ggtctggaac
     4141 tgctccagaa tggctccgtg atgttcaagg ggctccccgc ggcggatgtc ttgatgcggg
     4201 acttcaagga gttcgacctc ttcgatacgc gcatcggcgt ctcccaggct gtggtgttct
     4261 cgtttgaccg cattcgagaa cgggaaccgg agttcaagca ggcgatgcag gacatgcgga
     4321 cgcgcatggg gctggccatg gtggcttttc tggctacaga tcctctgagc aggacgtcat
     4381 acctgatcgt tgccgggcct tccgaggcgt tcgaggaggc atttgacgtg cagatggagg
     4441 acgggcacgc cgtcctggac ggaatactct cccggaagaa ggacttcatc ccgccagtgg
     4501 ccgaggtgct gtcgcgctac gcttgatccg gtccagggga gactggttga tcgaagtgtc
     4561 gtggaaccgt ggctccaggc tgcgagaccg gtcctggtag caggagaagg agtggggatg
     4621 ctcaacaagc agagtcatat gccgctgtac gtgcagctca aggatgagct cgccggtcgc
     4681 atcaagcacg gggcgatgaa ggtcggatcg cagatacaga ccgagtccga actcatgaag
     4741 cagtacggcg tcgggcgggc gacagtcaga gaagccgtgg cacttctggt cagcgagggt
     4801 tacctggtca agaggcaggg catcgggacg tttgtctgca acctgcagcc gtcgatgggc
     4861 ttcgagccgc tcatcagcct gacggcctcg ttgcgtgccc ggggcgtgaa tccccggaat
     4921 gtcgtcacca tcagcgagag attcgttcca ggggccgagc ttctggccca gctccgatgg
     4981 agcacccccg agacctgcac gcatgtgcgt cgcatccgct atgcggaaag tgcaccgatc
     5041 gcggtggagg actcgtggtt ttcgcacgag ttccaggatg tcggtgagaa gtacgatctg
     5101 acaggctctc tctcacgcat tatcattgag gacctgaagg tagacatcgc ccggatcgat
     5161 caggtcatcg tgccacgcct gccgtcgtca gaggagaagt ctcttctcga gttccaggga
     5221 gacgcacggg tcctgcagat ggaccgctgg atctacatcg tcggccggga gacgcctttc
     5281 tatacgcttc gattcatcgt tcccacagac atctactact cgctgggact ctagtcgacg
     5341 ccgtctccaa tcctgcggtt ccgcgtggct tgacacacac agacacactc cctatgctct
     5401 atacgtcagg acgtccgaac gtccgtacat atggggtgga aatgggcaga cggtcacagc
     5461 gcattcagca gaggcttcaa cagcaggtgg agcctggcat cagttgtcag gagggacatg
     5521 actcgatcat cctcgaggga tcagtcgaca actgggcaca ggtcgtgcgt gccggaaagc
     5581 tggctgctca cgaaggatac aagggagttg tgaacaggct tgcagtccgg gggttgacgc
     5641 tgcccgcacc ccgctccccg aagctgaaag acactgctct ggacggtcgt cacgtcgacg
     5701 tcctgatcgt gggtggtgga gtcaccggct gtgctcttgc gcgagagctt accaggaaga
     5761 agatctcggt cctgctggtc gagaaggaag acgatctcgc cacgcatgca tcatccagga
     5821 acaacgggat gatccatcca ggcatcgagc cgcacccggg cacgaggaag gcccacttca
     5881 atgtgcgagg caatgcaatg tacgataccg tggccgcgga gctggacgtt ccgtttgcgc
     5941 gatgtggatc gctcatcctc taccaggagc gctgggaacg ctttcttgga ccgctgttca
     6001 tggcgcgtgc caggcagaac ggagtgccgg gcgtgcggct gatcagcaag agtgaggcgc
     6061 tctcgctcga gccgcacctt acgcgtgagt tctcggctgc cctgcacatg ccttcgacag
     6121 ggatcatatc gccctatctg ctgacggtcg catacgccga gaacgccgta cgcaacggag
     6181 cggcgatcag tctcgagacc atggtcgtgg acatgaaggt gcgtgagggg cggatcacag
     6241 ccgtccgcac caaccggggc acgctgtatc ccagagtagt tgtgaacgcc gccggcctgt
     6301 atgcagacac tgtggcggac atggcgggtg accagttctt cacgatacat ccccggaagg
     6361 gcgagatcat catcctcgac cgcaagacgg gacatctcat ctcacatgtc gtcgcgaagc
     6421 cacgcctgct tcaagtgaag tctgacacca agggcggtgg catcaaccgg acgatagacg
     6481 gcaacatcct ggtgggtcca aacgccctgg aacagccatt ccgggaggac tacagcaccg
     6541 accgcgaccg tatcgagctg atgttacgcc ggtatctgcc cctggtcccc gagctgtctt
     6601 ctacagacct cataacgtac tttgcgagca cgcggcctgc gacctacgag gaggactttg
     6661 tcgtcgagcg gtcgcagtac gtgcggaacc ttgttcacgc ggccggcatc cagtctccag
     6721 gattggcgtc agctccggcc atcgcggcgg aggttgcccg gatgaccgcg gacgtgctgg
     6781 gagaagcgat cgaggtagct gacaatccga cctttgatgg ccgccgccac gggatccctg
     6841 atcctgagcg gatgacgtgg gaggagaggg atgccctcat ccaccggcgc tcggactacg
     6901 gtgtcatcgt atgcaggtgc gagggaatca gcaagggcca ggtcgtggat gcgctgaggt
     6961 ctccaattcc tgtggcaagc ctcgatgctg tcaagcgccg tgtccgggca ggcatgggac
     7021 ggtgccaggg cggattctgc acccctcagg tgctgggcat catcggcgaa cagaacgggg
     7081 gacagctgga ggccatcaca aagaagggag gcgactcctt cattctggcc accgagacca
     7141 agtcggcgca gagggcgcca tgatgctccg cagcgtcgac gttgctgtgc tgggggcagg
     7201 gccggcgggc atggctgctg ctctggctgc ggcccagggt ggggcgaaca cggtcctggt
     7261 agagcgggag aaccgtctgg gcgggatcct gaagcagtgt gtacacgatg ggtttggcct
     7321 gctgaggttt ggagagaagc tgtcgggacc ggagtatgca gggcgcttcg tccatatggt
     7381 gcgtgaagct ggtctcccag tcctgacgtc cacatttgtc gccgaggcac atcgagacgg
     7441 cgaagacaac atcctgatgc tcgtcgaccg cagtggtgtg accgagttgc acgcgaaggc
     7501 tgtcgtcctc gccacgggtt gccgggagcg gtctgcgaga caggtcatga tccagggcac
     7561 gcgccctgcg ggagtcatga ccgcaggggc cgcccagtac
//
LOCUS       DATMZD010000195         2329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_16156,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000195 DATMZD010000000
VERSION     DATMZD010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16156"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="VN478_04020"
     CDS             <1..651
                     /locus_tag="VN478_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21875.1"
                     /translation="VWNSVVDSWSVLPHDSAAPRRGGCLIAAADGWSLQSTLAVPNAL
                     SPRRALAVAATDGETALTIVSCYAPTNTGQGRKERPGYFSALAGLLSSVPAPVVLGID
                     ANGPRMDHPDTEQIKWWTREEALVLGAGARTEDALRLWYAGHPAELKRRTRYYPHGPL
                     ADSYHRGRKGKYLRCRYDSIRVSPGIRVLDVRYLYDDAIRAGSDHALVVTDIDLPE"
     gene            714..1601
                     /locus_tag="VN478_04025"
     CDS             714..1601
                     /locus_tag="VN478_04025"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HWQ21876.1"
                     /translation="MATDNVSAYFESIDRVTGYIEDHLDKPISLQEIAQVSGWSLWHF
                     HRLFHAVTGETIGEYVRLRRLHKAARVLTETRRPIIDIALDGQFESQEAFTRAFKRLY
                     GMPPGKFRRGGQQHPVFHKEPLTIDQLCHLQGGITMEPVIKTFKPVTVVGMEYHGQNK
                     NREITAMWDVFNQKCMAIPHLFNQRGAYGVCFSDADYGTTGMFRYVAGLPVGEVDVLP
                     EGMVSCTVPAGRYAVFTHCGMLATLSKTFDYIYGTWLPSSEYRLDAARPDFEYYDERF
                     TTGDDPASKFDIYIPITEK"
     gene            1819..2043
                     /locus_tag="VN478_04030"
     CDS             1819..2043
                     /locus_tag="VN478_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016518554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HWQ21877.1"
                     /translation="MERPKGKYLWTAKVGDKGQIVIPKEARDVFHIQPGDTLLLFGDE
                     TKGIAIAKYEAFTQFADTILKPQDPKEGDE"
     gene            2040..>2329
                     /locus_tag="VN478_04035"
     CDS             2040..>2329
                     /locus_tag="VN478_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002681925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWQ21878.1"
                     /translation="MNAIVMTGLTKKYGERTAVKSLSLSIEQGTCFALLGVNGAGKTT
                     TIKMLSCLTKPTSGDAVILGKSILTEPQAVKEIANISPQETAVAPNLTVRENL"
BASE COUNT          503 a          726 c          687 g          413 t
ORIGIN      
        1 gtgtggaaca gcgtcgtcga ctcgtggtct gtcctgcctc atgactctgc cgcccctcgc
       61 cggggagggt gcctgatagc ggctgctgac ggctggtcac tccagtcgac cctcgccgtt
      121 ccgaatgctc tcagccctcg ccgcgcgctt gccgtcgccg ccactgacgg ggagaccgcc
      181 ctgaccatcg tgtcctgcta tgcccccacc aacacgggcc agggcaggaa ggagcgccct
      241 ggctacttca gtgccctggc aggcttgctg tcctccgttc ccgcgccggt cgtccttggg
      301 atagacgcca acggaccacg catggaccat cccgacactg aacagatcaa gtggtggacc
      361 agggaagagg cgctggtcct tggcgccggc gcgcgaaccg aagatgctct gcgcctgtgg
      421 tacgctggtc atccggccga actgaaacgt cggacacggt actacccgca cggacccctc
      481 gccgactcat atcaccgtgg acggaagggg aagtacctgc gctgtcgcta cgacagcatc
      541 cgcgtctcac ccggcatccg ggtgctggat gtgcgctacc tctacgatga cgccatccgg
      601 gccggcagcg atcacgcgct ggtcgttacg gacatcgacc tgccagagta gcctcccgcc
      661 gcaacagcaa cccaggtcaa gaaatgcgtg cgaatgtagc tacattgaca tgtgtggcca
      721 ctgacaacgt atcagcatac ttcgagagta ttgaccgcgt gacgggctac atcgaagatc
      781 atctggacaa gccgatctca ctgcaggaga tcgctcaggt gtccggctgg tccctgtggc
      841 actttcaccg cttgttccac gcggtaaccg gagagacaat cggcgaatac gtccgcctgc
      901 ggcgcctgca caaggcagcc cgcgtgctca ccgagacgcg ccgcccgatc atcgacatcg
      961 cgctcgacgg acagttcgag tcccaggaag cattcacgag agcgttcaag cgcctgtacg
     1021 gcatgcctcc gggcaagttc cgccgtggcg gacagcagca cccagttttc cacaaggaac
     1081 cgctcaccat agatcaactc tgtcatctcc agggaggtat cacaatggaa ccagtcatca
     1141 agacattcaa gccggtcacg gtcgtcggca tggaatacca cggtcagaac aagaacaggg
     1201 agatcacggc gatgtgggac gtgttcaacc agaagtgcat ggcgatcccg cacctcttca
     1261 accagcgggg cgcctacggc gtctgcttca gtgacgcgga ctacggcacg accggcatgt
     1321 tccgctacgt ggccgggctc ccggtggggg aagtcgacgt cctgcccgag ggtatggtat
     1381 cctgtacggt gccagccggg cgatatgccg tcttcacgca ttgcggcatg ctggcgacac
     1441 tcagcaagac attcgactac atctacggca cctggctccc gtcgtcggag taccgactcg
     1501 acgccgcgag accagacttc gagtattacg acgaacgctt cacgaccggg gacgaccctg
     1561 cgtcgaagtt cgacatctac atccctatca ccgagaagta gctgaccgat ccccggcaga
     1621 tccgaccggg actcctgatc tccgagtcat tttttacagc aagggctgcg acgacatcgg
     1681 tcgcagccct ctgacgtctg ccctgatgct gctgctccag ggtctcctcc accttcctgt
     1741 tgacgcgcgg ggacgttagc agtatgatga gtcatacaaa acgcactcgg catacttggt
     1801 ggagcatgga ggtgaacgat ggagaggccg aaggggaagt acctctggac agcgaaggtc
     1861 ggagacaagg ggcagatcgt catccccaag gaagcgcgcg acgtctttca catccagccg
     1921 ggagacacgc tgctcctgtt cggcgatgaa acgaagggga tcgccattgc gaaatacgaa
     1981 gcgttcacac agtttgcgga tacgatcctg aaaccgcagg acccgaaaga aggagacgaa
     2041 tgaatgccat agtgatgacc ggattgacga agaagtacgg agaacggact gccgtcaagt
     2101 cgctgagcct ttcgatagaa cagggtacat gttttgccct gctgggcgtc aacggggcgg
     2161 gcaagaccac gacgatcaag atgctgtcct gcctcacgaa acctaccagc ggtgatgcag
     2221 tgatcctcgg caagagcatc ctgactgaac cacaggcggt gaaggagatt gccaacatct
     2281 ctccacagga gacggcggtc gcaccgaacc tcacggtacg ggagaacct
//
LOCUS       DATMZD010000196         5160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_146228,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000196 DATMZD010000000
VERSION     DATMZD010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5160
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146228"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VN478_04040"
     CDS             <1..207
                     /locus_tag="VN478_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012543825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper amine oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HWQ21879.1"
                     /translation="KDKVLELWIGKNTAKLNGQPVVIDTNGKVVPIISDGRAFLPLRF
                     VAEALALDVQWDAATRTVKISYGL"
     gene            723..3035
                     /locus_tag="VN478_04045"
     CDS             723..3035
                     /locus_tag="VN478_04045"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HWQ21880.1"
                     /translation="MRLSVRFFLMIMLCLVLGLSSVRSVPAAEAVEAATWKRLPLCGG
                     RVSCLAVDPSVSSTVYVGTITNGVFVSTDGGTTWSAASVGLEHQAVFTLVIDPVTPTI
                     LYAGTQEGWGPAGIFRSTDSGATWKKMSTTPEILSLAVDPAHPSTVYAGTGVGVYRST
                     DSGATWIEPDPYQAHMPNIAVHAVAIDPVVSSTLYTGTDNGVLRSNDSGTTWTAVNAG
                     LTNLVVLSLAVDPAHPATVYAGTEGGGVFRSATAGDRWTAMNAGLTISSAVVSIAISP
                     IAPFTVYAGTKGGGVFRSTTAGDRWTAVNSGLTNLAVLSLAVNPAHSATVYAGTEGGG
                     VFHSTTSGAVWTGPAFGIPDQQVTALAIDPVTPSILYVGTLWGDLFRSTDSGNKWTAA
                     HGGFHGADVYCLAIDPVTPSTLYAGTSTGIFRSTTSGSTWETTNAGITAPDVLALAVD
                     PATPSTLFAGTAYGVFRSTDSGKTWAASAYGYVASLAVDPADPRTVYACMESMVLRST
                     DSGTTWMELKNGLSGQHLSTLVIAPAVPSTLYVSAEGGIFRSTTRGSTWSKVSTWDHP
                     DPSYSGKLAIDPSDPSTLYTSTDGVLFRSTNGGATWASVMTTGASSNTALAIDRSDPS
                     VVYVATENVGVFRYRAPTGSIELTIGSSTMEVDGKPVSLEAAPVIINGRTFVPSRTLV
                     KALGGGVAWEASKRGLTVVHNGNQVDFRIGENAATLNWQSVAIDSDARVVPIIRDGRA
                     FLPLRFVAEALALDVQWDAATRTVKISYGL"
     gene            3384..>5160
                     /locus_tag="VN478_04050"
     CDS             3384..>5160
                     /locus_tag="VN478_04050"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWQ21881.1"
                     /translation="MTILSRRIPRILRSNGPVMVGSLLLVLLASMSYTGIEALVGNLA
                     LNRERFAQEYALESASFVTRSPLSNPRSLAETFHVVLEARREADVSIDALEPYTLRLM
                     EQTTQVNRAAVILGSTLDTDTDLLLGQTIAQARTLPVGSTIMIGGRALDVVGTVTLPD
                     YLYPLRDPEETSLLIDMKAFGIAVVTGAALDALGVPARVVWHVRGTALDLISLKYAVA
                     GSAGLATWLDTGVNPRYTIVDAEVKNARFAVASLPTLLLLLSSLMLAITMNRFVHSEL
                     PQIGMLLAQGYTAGELLCHYLALPSVVAGAGSVLGSVAGLLVFKPLYSFYTLFFAVPL
                     LHFEIRAIWLLLSVLLPFAFLLPSVVLVVWRAVRRTPVELMRNRGERVQISAIEKHIR
                     MRHLSAAAQFRLRDVVRSPARLIVTFVGIAAAGAMLLAGQTLQHSITDMIETTFDETM
                     RYDYQYVLRDVRTANPWDAETAYVESFRGTRPGDPGLQLMGVDPGFGMIRAVDTSGVA
                     VSFDQVVVTRPLADRWNLAPGDTVAIIRTATDTSYSLKIDSVAEIYTNSAVIMPRDQM
                     TALLGMPLHALNVVYSMDPLPIADAE"
BASE COUNT          955 a         1547 c         1571 g         1087 t
ORIGIN      
        1 aaggacaagg tgctggagct ctggatcggc aagaacacgg ccaagctcaa cggtcagccg
       61 gtcgtcatcg acacgaatgg caaggtcgtt cccatcatca gcgacggaag ggcgttcctg
      121 cctctccgct tcgttgcgga ggcgcttgcg ctggatgtcc agtgggatgc ggctaccagg
      181 acggtgaaga tctcttacgg actgtagcgc gggaacgaca ccaaccgggg aagaagtggc
      241 agaattgcag gggttctgcg gattgtctct gggttgttga gcaggtcgag agaggctccg
      301 tcactgaata tgtactggcc gacacggtgc aaacccagta gcgatggggt attggtggaa
      361 ccgaggggca tggccctccc gaagaccgct ctgttgacgt tctcgtcagc attgtccaag
      421 cttggctgct ggagaagtcg catgtctcat agaccaattc ggcgtgatgg ccggacgacg
      481 ggcatcggga agatgcatat gtctgcaccg gacaactgac ggaaacgcgg ccaggtacag
      541 aacaggtcgc agtgttcgcc tttccgcgtg agttaacgat gaattaacgt tggcatggta
      601 gaatacgcgc aagggcagcc gtgacctggt gtttcgcact tgtgacaggt ttccgtcagt
      661 tctgatcgtc gtgatcagca gtttgctgcg attcatgcca acgcccaaag gaggccgcaa
      721 ccatgagact atccgttcgt ttctttctga tgatcatgct gtgtcttgtc ctgggcctct
      781 cgagcgtacg tagcgtccct gccgcagagg cagtggaggc cgcgacctgg aaacggctgc
      841 cgctttgcgg aggtcgggtg tcgtgccttg ctgtggatcc gagtgtctcc tcgactgtct
      901 acgtcggcac gatcactaac ggtgtcttcg tctcgacaga cggcggcacg acgtggagcg
      961 ccgcaagcgt gggacttgag catcaggcag tcttcacact tgtcatcgac ccagtcactc
     1021 cgacgattct gtacgccgga acccaggaag gttgggggcc cgcaggcatc ttccgctcca
     1081 cagacagcgg cgcaacgtgg aagaagatga gcacgacccc ggagatcctg tctctcgcgg
     1141 tcgaccctgc tcacccttcc accgtttacg ctggcacggg cgtcggtgtg tatcgctcca
     1201 cagacagtgg tgcaacgtgg atcgaacccg acccatatca ggcgcatatg ccgaatatcg
     1261 cggtgcacgc agtcgcgatt gaccctgtcg tttcttccac actctacacc ggcaccgaca
     1321 atggtgtcct tcgctccaac gacagcggaa cgacatggac ggccgtgaac gcggggctca
     1381 ccaatctggt tgtcctgtct ctcgcggtcg accctgctca ccctgccacg gtgtacgccg
     1441 ggacggaggg tggcggtgtc ttccgctcag cgaccgccgg ggaccggtgg actgctatga
     1501 acgctggact caccatttcc agtgctgtcg tttcgattgc gatcagcccc atcgcccctt
     1561 tcaccgtgta tgcaggcacg aagggcggtg gcgtcttccg ctcaacgacg gctggagacc
     1621 ggtggaccgc tgtgaacagc ggactcacca acctggccgt cttgtctctc gcggtcaacc
     1681 ctgctcactc tgccaccgtg tacgccggca cagagggcgg cggcgtcttt cattccacga
     1741 cgagcggcgc ggtgtggacc gggccggcct ttgggatccc cgatcagcag gtgacagctc
     1801 ttgcgatcga tcctgtcacc ccctctatcc tctatgtggg gacactctgg ggggaccttt
     1861 tccgctccac cgacagcggc aacaagtgga ctgcggcgca cggcggcttc catggtgccg
     1921 acgtatactg tcttgcgatc gatcctgtta caccttccac gctgtatgcc ggcacaagta
     1981 ccgggatatt ccgctccacg acgagcggct cgacatggga gaccaccaat gcaggaatca
     2041 ctgccccgga tgtcctagcg cttgccgtcg accctgccac tccttcgacc ctgttcgccg
     2101 gtacggctta cggcgtcttc cgctcgacag acagcgggaa gacgtgggct gcgagcgcct
     2161 atggatatgt cgcgagtctt gctgtggacc cggctgaccc tcgtaccgtc tacgcgtgca
     2221 tggaaagcat ggtcctccgc tccaccgaca gcgggacgac atggatggag ctgaagaacg
     2281 gactctccgg tcagcatctc agcactctcg tcatcgcccc tgccgtccct tccacgctgt
     2341 acgttagtgc tgagggcggt atcttccgct ccacgacgag aggatcaacg tggagcaaag
     2401 taagcacctg ggatcatcct gatccttcat actctgggaa gctcgccatc gatccctctg
     2461 acccgtccac actctacacc agtactgatg gtgtcttgtt ccggtccaca aatggcggtg
     2521 cgacatgggc gtcggtcatg accaccgggg cgtccagcaa cacggctctg gccatcgacc
     2581 gcagtgaccc ttccgttgtc tatgtcgcca cggagaatgt cggtgtcttc cggtacaggg
     2641 ctccgacggg cagcatcgag ctgacgatcg ggagcagcac gatggaggtg gacgggaagc
     2701 cggtgtcgct ggaggcagcc cctgtcatca tcaacgggcg cacgtttgtc cctagccgca
     2761 cgctcgtgaa agcactggga gggggcgtcg catgggaagc atcgaagcgg ggcctgaccg
     2821 tcgtgcacaa tggcaaccag gtggactttc gcattgggga gaacgcggcg actctcaact
     2881 ggcagtcggt cgccatcgac agcgatgcca gggtcgttcc catcatcagg gacggaaggg
     2941 cgttcctgcc tctccgcttc gtcgcggagg cgcttgcgct ggatgtccag tgggacgcgg
     3001 ctaccaggac ggtgaagatc tcctacggac tctagcgcga acggcagcag ccggggcaga
     3061 agcgggccac tggtgtacga cccatgttct cttgactcct gacattgcga cgcaatactg
     3121 gcatcacaag cgaacgatgg tcgtcgccgc atctcgcggg cacatgcggc tgaagacgcg
     3181 ggcaacgcga ggcttccgtt tgcaggcgga tgcagactat cgccgctgta gaggagttgc
     3241 agggtgtcga gactactgga cttgatcctg agtgttgtca tcgcgattgc tttcctggta
     3301 ataggtcact ggctggggaa gaaggccaag tagagcagca ggagagagct gtctgacatc
     3361 ggctgtcgtg gaggaaccgg cctgtgacga tcctctccag acgcattccg cgcatactgc
     3421 gctccaatgg tcccgtcatg gtggggtcac ttctcctcgt gctcctggcg tcgatgtcct
     3481 acacgggcat cgaggcgctt gttggcaact tggcccttaa ccgtgagcgg tttgcccagg
     3541 agtacgcact tgaatcggcc tccttcgtga cgcgaagccc gctctcgaac cctcggtcgc
     3601 ttgccgagac cttccacgtg gtattggaag cgagacgcga agccgatgtg tcaatcgatg
     3661 cgctggagcc gtacacgctg cgtctcatgg aacagacgac gcaggtcaac agagctgctg
     3721 tgatcctggg ctcaaccctt gacactgata ccgacttgct gctcggccag acgattgcgc
     3781 aggcgcggac tctgccggtg ggaagtacaa tcatgattgg cggtcgagca cttgacgtgg
     3841 tcgggactgt cacgctaccc gactatctgt atcccctgag ggatccagag gagacgtcac
     3901 tgctgatcga catgaaggct ttcggtatcg cggtcgtgac tggtgcagcg ctcgacgcgc
     3961 tgggggttcc agccagggtc gtgtggcacg ttcgtggcac cgccctagat ctgatctccc
     4021 tcaagtacgc cgtcgcaggc tcagctggct tggcaacgtg gctggacact ggcgtgaatc
     4081 cccgttacac gatagtcgat gccgaggtca agaatgctcg ttttgcagta gcatcgctgc
     4141 cgacactgct tctcctgctt tcgtcgctga tgctcgcaat cacgatgaac cggttcgttc
     4201 actctgaact cccccagatc ggcatgctgc tggcgcaagg ctacacggca ggcgagctgt
     4261 tgtgccacta cctggcgctg ccttctgtag ttgccggtgc cggctccgtg cttggcagtg
     4321 tagccggact ccttgtcttc aagccgctct acagcttcta cacactcttc ttcgccgtgc
     4381 cccttctgca cttcgagatc cgcgcgatat ggttactgct cagtgttctt ctgccgttcg
     4441 ccttcctgct tccatcagtc gtattggttg tgtggcgtgc ggtgcggcgc acgcctgtgg
     4501 aactcatgag gaatcgggga gagcgtgtcc agataagtgc aatcgagaag cacatacgga
     4561 tgcgccacct gtctgcagcc gcccagtttc gcttgcgcga tgtcgtgcgg agccccgcgc
     4621 ggctcatcgt gacattcgtc gggattgcag ccgcaggagc catgctgttg gcgggacaga
     4681 cgctgcagca ttccatcacg gacatgattg agactacctt cgatgagacc atgcggtacg
     4741 actaccagta tgtgttgcgt gatgtccgca cagccaatcc atgggatgca gagactgcct
     4801 atgtcgagtc ctttcgcgga accaggccag gcgaccccgg cctgcagctc atgggagtgg
     4861 atcctggatt tggcatgatc cgggcagtcg acacgtcggg agtggcagtg tcgtttgacc
     4921 aggtggtcgt gacgcgcccg ttggcagatc ggtggaatct ggctcctggt gataccgtcg
     4981 ctatcatccg gacggccaca gatacgtcgt atagcctgaa gatcgattct gtcgccgaga
     5041 tctacaccaa cagcgccgtt atcatgcctc gcgaccagat gacggctctc ctgggaatgc
     5101 ctctccacgc actcaacgtt gtctacagca tggacccgct tcccatcgcc gacgcagaac
//
LOCUS       DATMZD010000197         5805 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_45541,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000197 DATMZD010000000
VERSION     DATMZD010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5805
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45541"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VN478_04055"
     CDS             <1..392
                     /locus_tag="VN478_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21882.1"
                     /translation="EPSAAGEDASLQVLLEFAKANVGLSETVNRLLKSGNQSGLRDNA
                     MASLQTLAVFMNSVLRARGKVSPAAGISLSTEPELARVNFVLDESRVGGFLDHLGERL
                     KHIEQNVNPELVLTNALLELRRMTTHE"
     gene            472..1122
                     /gene="ricT"
                     /locus_tag="VN478_04060"
     CDS             472..1122
                     /gene="ricT"
                     /locus_tag="VN478_04060"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HWQ21883.1"
                     /translation="MVEVGDSVTLLPGDEVLAVGSDKELMHVIIGYPRHDATGKKRTA
                     GGLKFVRKLAVTDVERVEENRRKQDAAVVLCKEQIRAIKLPIHLVNCELSFSGKQYVF
                     YFTAETRVDFRTLVSTLSRSLKAKVQMWQIGSRDETRFFATVGMCGRELCCNCFLKEI
                     KPVSLKCARVQNLSLNPNKITGVCGKLMCCLRYETYEYGDAAGLDVRKKPKEGTGQ"
     gene            1219..2253
                     /gene="mnmA"
                     /locus_tag="VN478_04065"
     CDS             1219..2253
                     /gene="mnmA"
                     /locus_tag="VN478_04065"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HWQ21884.1"
                     /translation="MSVVVGMSGGVDSAVSALLLKELGERVIGVTLHFSERSACCDVG
                     STRRAKAQCEQLGIAWHSVDAKDAFTCRVIEPFWQAVSRGITPNPCVFCNEKVKWQGL
                     LDAADELHADFVASGHYAGIAHSADGDQICRGLDHVKDQSYFLYRLSAEQRRRILFPL
                     AGRMKSEVVTLASRWFDPALLARRESQDLCFVEGSFGEDSRHRFPCTPGDIILANGTV
                     VGRHHGLTPFTVGQRSGLGISASSRLYVIEKRHDSNQLVVGSRSECTEDEFEAVDLKW
                     QHLPTGNAECFTADVVTRYRSKPVSGRIQRVSGDRVSVHLADPVFAVTPGQAVVFYAG
                     QRILGGGTIV"
     gene            2319..2762
                     /gene="mraZ"
                     /locus_tag="VN478_04070"
     CDS             2319..2762
                     /gene="mraZ"
                     /locus_tag="VN478_04070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HWQ21885.1"
                     /translation="MNKENCFIGEYRYSVDDKGRTMFPAHYRCDTGSPYVLTKGMDRC
                     LYLFDDRVWTSIIERFYGDLIPVSSDNRAFVRILFSGAATVELDKIGRISIPQHLREY
                     ASLDREVVFAGALTHLEIWDVHIWDTYRSKAEKVFEQNIGALQAR"
     gene            2759..3661
                     /gene="rsmH"
                     /locus_tag="VN478_04075"
     CDS             2759..3661
                     /gene="rsmH"
                     /locus_tag="VN478_04075"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HWQ21886.1"
                     /translation="MSEFAHTPVLLDEVTTYLQIRPNGTYLDCTTGEGGHAYEVGRQL
                     SSEGTLFMIDVDAEVLAIARERLKDTAARKVIIRGNFRDMTRLLKDYPDTRFDGILMD
                     LGFSSEQLTGTGRGFSFSHDQPLDMRLDDDLKVTARNIVNSFPEEDIADILWKYGEEG
                     RSRQVARAIVRRRQRAPIETTSELAETVAAVFPTRHARIHPATKSFQALRIYVNDELN
                     ALDNGLKSAASLLNLGGRLLVISYHSLEDRIVKRSYLALCENPVSGEPGFHLVNKKVI
                     RPTDSEVATNKRSRSAKLRVLERA"
     gene            3664..4008
                     /locus_tag="VN478_04080"
     CDS             3664..4008
                     /locus_tag="VN478_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21887.1"
                     /translation="MSIAVRGNYTTIRRPVAAVRVSAEARVAVRRYIVLGATVVSLLC
                     AYGYVYSTTLFLESQVDLNRTAIQAQLQQQESLLERDTRLMSPDRVRAAASALGMAAN
                     NTAVIGTLDSLH"
     gene            4042..5748
                     /locus_tag="VN478_04085"
     CDS             4042..5748
                     /locus_tag="VN478_04085"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HWQ21888.1"
                     /translation="MKRGVASFNNLFRRRLVIVFAVFLVAVLGIEARFIYLQVVARTT
                     LVDMANQQLETDSIPIKAKRGTIQDSTGRILAQDVATWSLGLYMREVKDPHLTAVSIA
                     PILKASAAQLEKQIRSASSTFIYVKKQVGVGEYEALRRLDVPGIVLDKGYRRVYPLGS
                     LISSVVGVTGSDNQGLSGIEFQYDKELKGTDGRHTYRVSGSNPGEPAVTEEVTEAIAG
                     KDIVLTIDSNIQYFAQQALMDTLKAYDGKRATCTILDPKTDAVLAMVDVPGVDPEHWQ
                     EASMSSLAEKVVSANYEPGSVFKAVTSVAGLETGTVTLQSTFYSGGSLKVFDTVVHDL
                     YPYGTMNLYAMLQHSSNVAFCQLSMKIGAKPFYDCVTRYGFGKPTGIDLPGEESGILL
                     PTSLWSKTTLPTMGFGQGVAVTAIQMANFYATIANGGVMQRPYVVDHIIDASGASQRT
                     DTPGVVGQLFKPETAKAVLEGLHGVVQKDVGVFSAYIPGYQVAGKTGTAQIAAPGGGY
                     LSDGSYVYSFAGILTANDPRVVVFLSVHETRKGARTSFSVAGPTFKKIALQLIQYLRL
                     KP"
BASE COUNT         1294 a         1558 c         1715 g         1238 t
ORIGIN      
        1 acgagccgtc cgccgctggc gaggatgctt cccttcaagt cctgctggag ttcgccaagg
       61 caaatgtagg cctctccgag acggtcaacc gtctcctgaa gtcagggaat cagagcggtc
      121 ttcgcgacaa cgccatggcc agtctgcaga cactggcagt gttcatgaac agcgttctga
      181 gggccagagg aaaggtctct ccagcggcag gaatttcgct ctcgaccgaa cctgaactgg
      241 cacgtgtgaa cttcgtattg gacgagtcca gagtcggcgg ctttcttgac catcttggag
      301 agaggctgaa acacatagaa cagaacgtca atcctgaact cgtcttgaca aacgctctgt
      361 tggagctgag gagaatgacc actcatgaat gatcccacat atatgctacc ggccagatgc
      421 tcgtcagttc gctatctgag cgctcgctca gccaagtcat atcagacgta catggttgaa
      481 gtcggcgaca gcgtgacgct gcttcccgga gacgaggttc ttgcagttgg cagcgacaag
      541 gagctgatgc acgtgatcat agggtatccc aggcacgatg ccacgggcaa gaagcggacg
      601 gcaggaggtc tgaagtttgt ccgtaagctt gctgtcaccg acgtcgagcg tgtcgaagag
      661 aatcgccgca aacaagacgc cgccgtggtt ctgtgcaaag aacagataag agccatcaag
      721 cttcccatcc acttggtcaa ctgcgaactg tcgttctcgg gcaaacagta tgtgttctac
      781 ttcactgccg agactcgagt cgacttccgg actctggtct ctactctgtc gcggtcgctg
      841 aaggcaaagg ttcagatgtg gcagataggg tctcgcgacg agacacgttt cttcgcgacg
      901 gtcgggatgt gcgggcgtga gctgtgctgc aactgtttct tgaaagagat caagccggtt
      961 agtctgaagt gtgcacgggt acagaacttg agcttgaatc ccaacaagat aactggcgtc
     1021 tgtggcaaac tgatgtgttg cctcagatac gagacctatg aatacgggga cgcggcaggg
     1081 cttgacgtca ggaagaaacc gaaggaaggg accggccagt agtgcatgcg ggcagcatgc
     1141 cggtagactc tctccggcag tcacaacgac cgcggccgct atgagtccac aagtgcgccc
     1201 ggtgctcggg gtttgtccgt gagcgttgtc gtcgggatga gtgggggcgt agactccgcg
     1261 gtctctgcat tgctcctcaa ggagcttggc gaacgtgtca tcggcgtcac tctacacttc
     1321 tccgagcgca gtgcgtgttg tgacgtcggc tctactcgac gtgcgaaagc gcagtgtgag
     1381 cagttgggaa tcgcgtggca cagcgtggat gcgaaggacg ccttcacttg cagggtgatc
     1441 gagccgttct ggcaggcagt atcgcgagga atcactccga atccctgtgt gttctgcaac
     1501 gagaaggtca agtggcaagg cctgctggac gcggccgatg agctgcatgc cgatttcgtc
     1561 gccagtggcc actacgctgg aattgcccat agtgccgatg gagaccagat ctgtcgcggc
     1621 ctggatcacg tgaaggacca gtcgtacttc ctgtaccgtc tctctgcaga gcaacgccgc
     1681 agaatcctgt ttcctcttgc gggccgcatg aagagcgaag tggtgacgct ggcttcgcgc
     1741 tggtttgacc ccgctctcct ggcaagacgc gagagtcagg acctgtgttt tgtggaagga
     1801 tcctttggcg aggattcccg tcatcgcttc ccatgcactc ctggagacat tatcctcgcg
     1861 aacgggacgg tggtcggccg gcatcatgga ctgacgccgt ttacggttgg ccagcgatcg
     1921 ggacttggca tcagcgccag ctcacgactg tacgttatcg agaagcggca tgactcgaac
     1981 cagctggtcg tggggagcag gagtgaatgc acagaagatg agttcgaagc tgtcgatctg
     2041 aagtggcagc accttcccac cggcaatgcg gagtgcttca ccgcggacgt cgtcacacga
     2101 tacaggtcaa agccagtcag cggccgtatc cagagggttt ctggcgatcg agtctccgtc
     2161 catttggctg atccggtatt tgccgttact ccgggacagg ccgttgtctt ctatgctgga
     2221 cagcgtatac tcgggggagg aacgatagtc tagaacttgt tcttgacgag ttatcacgaa
     2281 ttgtggtaaa aagtgggaaa ttgtggtata cttacgccat gaacaaggag aactgtttca
     2341 tcggcgaata ccgctactcc gtggacgaca agggacgaac gatgttcccc gcccactacc
     2401 ggtgcgacac tggttctccc tacgttctca ccaagggcat ggatcgatgc ctttacctgt
     2461 ttgatgacag agtgtggacc tccatcatcg aacggttcta tggtgacctc atccctgtct
     2521 catctgacaa tcgggccttc gtccgcattc tcttcagcgg agccgcaacg gttgaacttg
     2581 acaagatcgg tcgcatttcc atacctcagc acttgcgtga gtatgcttca ctcgacagag
     2641 aggtcgtatt cgccggtgct cttacgcacc tggagatctg ggacgttcac atctgggata
     2701 cctatcggtc taaggcggag aaggtgttcg aacagaatat tggagctttg caggcaagat
     2761 gagtgagttt gcacataccc cggtcctcct ggacgaggtc acgacatact tgcagattcg
     2821 accgaacgga acctatctgg actgcacgac aggggaaggc ggacacgcat acgaggttgg
     2881 gcgccagctc tcgagcgaag ggacgctttt catgatcgat gtcgacgcag aggttcttgc
     2941 cattgcccgc gagcggctga aagatactgc cgcccgcaag gttatcatcc gcggaaactt
     3001 tcgggacatg acgcggttgt tgaaggatta tccggacact cgattcgacg gaatcctgat
     3061 ggacctgggt ttttcttcag agcaactcac gggcacaggc agggggttct cgttcagcca
     3121 tgaccaacca ctggacatgc gactggacga cgatctcaag gtcactgcga ggaatatcgt
     3181 gaactcgttt ccggaggaag acatcgccga catcctgtgg aagtacggcg aggaggggag
     3241 gtcgcgtcag gtcgctcgcg cgatcgtgcg ccgtcggcaa cgggcaccga tcgagaccac
     3301 gagcgaattg gccgagacag tggccgctgt cttccctacc agacacgccc gcatacatcc
     3361 tgccaccaag tcctttcaag ccctgcgcat atatgtcaac gatgagctca acgctctcga
     3421 caatggtctg aagtccgcag catcgctctt gaatctaggt ggaagactcc tggtcatcag
     3481 ttaccattcg ctcgaagata gaattgtgaa gagaagctac ctggctctct gcgagaatcc
     3541 ggtctcgggg gagccaggat ttcacctggt caacaagaag gtcattcggc ctacggactc
     3601 agaagtcgct acgaacaaac gctctcgcag cgccaagctg cgcgtgctgg aaagggcata
     3661 accatgagta ttgcagtcag aggaaactac accacaatac gccgtcccgt ggcggctgtt
     3721 cgtgtatcag ccgaggcaag agtcgctgtt cgccggtata tcgtcctcgg tgcaaccgtc
     3781 gtcagtctac tgtgcgccta tggctacgtg tatagtacga ctctgtttct ggagagtcag
     3841 gtggatctga acaggacagc tatccaggct cagcttcaac agcaggagtc actgctcgaa
     3901 cgtgatacca ggcttatgtc acccgatcgg gtgcgagctg ccgcaagcgc cttgggcatg
     3961 gcagccaaca acacggcggt gatcggcaca ctggacagtc ttcactagca ggaatacctg
     4021 cgtcaagatc ataggggatt gatgaagaga ggcgtcgcgt ctttcaacaa cttgtttagg
     4081 cgacgtctcg tcattgtttt tgcggtgttt ctggttgcag tgctaggaat cgaggcacgg
     4141 ttcatctatc tgcaggtagt ggctcgcacg actctcgtgg acatggccaa ccagcagctc
     4201 gaaaccgact ccattcctat caaggcaaag cggggaacga tacaggattc gacagggcgc
     4261 atcctggcac aagatgtggc cacatggtct ctgggcctgt acatgagaga ggtcaaggat
     4321 cctcatctga ccgcggtcag catcgccccc atcctgaagg cgtcagctgc acaactggag
     4381 aagcagataa ggagcgcatc gtctaccttc atctatgtca agaagcaggt aggcgtgggg
     4441 gaatatgaag ctctccgtcg gcttgacgtg ccgggaatcg tgctggacaa gggctatcgc
     4501 cgtgtctacc cactcggatc cctcatttct tcggttgtag gcgtcacggg ctcagacaac
     4561 cagggtctct cggggatcga gttccaatac gacaaggaac tcaagggaac tgacggacga
     4621 catacctaca gggtgtcggg atcgaatcca ggcgaacccg ccgtgacaga agaagtcacc
     4681 gaagccatag cgggcaagga tatcgtgctg acgatcgact cgaatatcca gtacttcgcg
     4741 cagcaggcgc tcatggacac gctgaaggca tatgatggca agcgagccac gtgcactatc
     4801 ctggacccaa agacagatgc agtcctggcg atggtcgatg tgccgggcgt ggatcccgaa
     4861 cactggcagg aagcctcaat gagctcgctt gcggagaagg tggtcagtgc caactatgag
     4921 ccggggtcag tgttcaaggc agtgacgtcg gttgctggac tggaaacggg cacagtcact
     4981 ctccagagca ccttctacag tggcggaagt ctcaaggtat tcgacacggt ggtccacgat
     5041 ctgtatccat acgggaccat gaacctctac gcgatgctgc agcactcgtc caacgtagcg
     5101 ttctgccagc tgagcatgaa gattggtgcc aagccattct acgactgcgt cactcgatat
     5161 ggcttcggca agccgacggg catcgatctc ccaggagagg aatctggcat ccttctgcct
     5221 acgtctctct ggtcaaaaac gactctcccc acgatgggat ttggccaggg cgtagcggtg
     5281 actgcaatcc agatggcgaa cttctacgcc acgattgcca atgggggcgt catgcagcgt
     5341 ccctacgtcg tcgatcacat catcgacgct tcgggagcaa gccagcgcac cgacactcct
     5401 ggcgtcgtcg gccagctgtt caagcccgag actgcgaagg ccgttcttga gggcctgcat
     5461 ggagtcgtgc agaaggatgt cggagtattc agcgcctaca tcccgggcta tcaggtcgca
     5521 gggaagaccg gtaccgccca gattgcggct cctggaggag gatacctgag cgatgggtcc
     5581 tatgtctaca gtttcgctgg catcctgact gcaaacgacc cgagagttgt cgtgtttctc
     5641 agtgtccacg agacccgaaa aggggcgcgg acgtcattca gcgttgcagg tccgaccttc
     5701 aagaagattg cgcttcagtt gatacagtat ctgaggttga aaccatgaaa ttcgcgactg
     5761 aaggacggga gcttgttgag ctccttcata tgttgccagg tggct
//
LOCUS       DATMZD010000198         4197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_16214,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000198 DATMZD010000000
VERSION     DATMZD010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16214"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..450)
                     /locus_tag="VN478_04090"
     CDS             complement(22..450)
                     /locus_tag="VN478_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21889.1"
                     /translation="MKRILLVLVIVACSMFAGCAALQEPIMPTVTVASRNVPVRLGTY
                     GWRSPGQGIQSDAPVPDEFVVDVQAVSAPCGSSVSVDYGRRPGDLFAYVWNDHEPTRV
                     EIQGGTFTLPLDPGTYIYSLESTWREGHASHVIKLEAIPR"
     gene            complement(531..1115)
                     /locus_tag="VN478_04095"
     CDS             complement(531..1115)
                     /locus_tag="VN478_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21890.1"
                     /translation="MNRKRMRTFVTIIIVLALVSGGAVTLISALRGTTVDEPVTVLPG
                     VTLSPQDLQGVGLGTAVGVQYAGKTFVFYADSSGGELAGTKSGMAIDFRGGSVTFLGG
                     QFTQYDSPTVRDQAYAWYRDKVASAGLVDDKGTSVVGDASARYSDPDVAALLVRKNDT
                     VFTLLYTLGTAATNDTPRPIVALEALARLLASRF"
     gene            complement(1173..1868)
                     /locus_tag="VN478_04100"
     CDS             complement(1173..1868)
                     /locus_tag="VN478_04100"
                     /inference="COORDINATES: protein motif:HMM:NF017655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraX family protein"
                     /protein_id="HWQ21891.1"
                     /translation="MTSGDADTLSAEKGQFTLEQSDALKLIAIICMTVDHVGAILLPQ
                     AGWLRIIGRVAFPLFACQLAVGYMHTHNLPRYVLRLAIWGLIAQPIYMIAFDVRPWTF
                     NIFGTLLLGLLAIWGWDQHRWWAVVFALSLAAVQLWLRAVGPDYGVYGVLLCLVSFVL
                     SRDRRQLAVGHALLHWLAGIMFWPSQVYAVVSIPLILWPPRLHLGRLSRLFYAYYPAH
                     LALLVLIRLLLAR"
     gene            1944..2018
                     /locus_tag="VN478_04105"
     tRNA            1944..2018
                     /locus_tag="VN478_04105"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1976..1978,aa:Gln,seq:ttg)
     gene            complement(2125..3123)
                     /locus_tag="VN478_04110"
     CDS             complement(2125..3123)
                     /locus_tag="VN478_04110"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HWQ21892.1"
                     /translation="MAVTYYLGVDGGTTRTKAVVGDDVGHVAGSGEGGASNYQVVGLE
                     AAMSGVAEAVQNALTAAGLSIAQVERAVFGLSGMDLPMHREALDSALTAAFPGLIFDL
                     VNDTWIMLKAGTDKGWGIALVCGGGANACACSRDGTWVTLRGLGYESGLRGGGLDMLR
                     DILHVAFLSHDGTGPKSVLEQTVLEVTGAPDYDALQLMFLETVQDMVGHGELLHRALA
                     VVPLVFDGATAGDDACRRILILQAESLAEGIIGLIHKMRFEHEPVDVVLGGSVFGGGN
                     PVFVDKLELLVHEVAPLAQLQLPLLDPVLGAYVMALQKMGKFDAASTYGVLAGQVV"
     gene            complement(3125..3865)
                     /locus_tag="VN478_04115"
     CDS             complement(3125..3865)
                     /locus_tag="VN478_04115"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HWQ21893.1"
                     /translation="MNWNEFLPVPPIDSAQKYVVFQPHSDDAVWFAGGLLLKLTRAGK
                     DVTLVTMTDGCLGSIDVSMTREHLVEIRRAEDQKAGEAMGVTRHLYLPFHDGELPHCG
                     ETTAAMVRIIRQERPDAVLAPDPWLAYEAHQDHLNCGWSAAESLIYCSLPLYLPELPP
                     HDVRFIAFFLTGRPNQFVDVSAENETRLAALSLFKSQFTPDTLEMATQYLNLKAAELG
                     EGRGMQLAEAYKVLTPLHLHTSVDSENI"
     gene            complement(3882..>4197)
                     /locus_tag="VN478_04120"
     CDS             complement(3882..>4197)
                     /locus_tag="VN478_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21894.1"
                     /translation="YGVNALIIRMSVAISAGTISFVQTITRYKPVTDPTLMAPTAVLG
                     FRLLISVVPALLIAIGLIFVFLYPLHGKKLEDVKAQCALSTSSCPESPGPGTPFIGTP
                     AA"
BASE COUNT          826 a         1280 c         1335 g          756 t
ORIGIN      
        1 ggtcagtcca gctagagagg tctacctggg gatagcctcc agcttgatga cgtgactggc
       61 atgtccctcg cgccacgtcg attcgaggct atagatgtag gtgccgggat ccagaggcag
      121 cgtgaacgtg ccgccctgga tctcgacacg tgtgggctca tgatcgttcc agacataggc
      181 gaacaggtcg cccggtcggc ggccgtagtc gactgacact gaggatccac acggcgcact
      241 gactgcctgg acgtcgacga caaactcgtc cggcactggg gcatcagact ggatcccctg
      301 tccaggggat cgccatccat aggtacccag acggacgggc acgttgcggg aagcgacggt
      361 cacagtaggc atgatcggtt cctgcagtgc ggcacagccg gcgaacatgg agcaggcgac
      421 tatgaccagg accaacagta tgcgtttcat ggatccctcc tgacggccgc ggcatagcac
      481 gccacagcac actactgtga cggccctggc ggtcggagtg ttcccggcag ctagaagcgt
      541 gaagccagca ggcgggcgag cgcctcgagg gcgacgatcg gtcgaggtgt gtcgtttgtg
      601 gcagcagtac ccagtgtgta caggagcgta aacaccgtgt cgttctttcg tacgagcagt
      661 gctgccacgt cagggtcgga gtaccgtgcc gaggcgtccc cgacgaccga ggttcccttg
      721 tcgtctacca gccccgcgct ggctaccttg tctctgtacc aggcatacgc ctggtcgcgg
      781 accgtgggac tgtcgtactg agtgaactgt cctccgagaa acgtcacgga gccgccacgg
      841 aagtcgatgg ccatgccgct ctttgtcccg gcgagttcac ctccggaact gtcggcatag
      901 aagacgaagg tcttgccggc gtattgcacg ccgacggccg ttcccaggcc cactccctgc
      961 aagtcctgtg ggctgagcgt gacaccgggg aggacagtaa cgggctcatc cacggtcgtg
     1021 cctcgcaggg ccgagatgag tgtgactgcg cctccgctga cgagagcgag gacgatgata
     1081 atggtgacga atgtgcgcat gcgtttgcgg ttcacggttg cctccaggtg ctggccatca
     1141 gtatagggcg ggtcccggct cgaacaatgt cgtcatcgtg ccagcagaag acggatcagc
     1201 acgagaagag ccaggtgagc cggatagtat gcgtagaaca atcgtgacag acgaccgagg
     1261 tgtagtcggg gcggccacag gatgaggggg atactgacca ccgcgtagac ctgggacggc
     1321 cagaacatga taccggcaag ccagtgcagg agtgcgtgac ccacggcgag ttgccgtctg
     1381 tcccgggaca ggacgaaact caccaggcac agcaggacgc catagacgcc atagtccggg
     1441 cccaccgccc gcagccagag ctgtacggcg gccaggctca gcgcaaagac gacggcccac
     1501 cacctgtgct ggtcccatcc ccagatggcc agcagtccca gcagcagcgt gccgaagatg
     1561 ttgaaagtcc acgggcgcac gtcgaatgcg atcatgtaga tcggctgagc gatgagaccc
     1621 cagatggcaa gcctgagcac gtagcgcgga aggttgtggg tgtgcatgta gccgacggca
     1681 agctggcagg cgaagagggg aaaagccacg cgtccaatga tgcgcagcca gcctgcctgt
     1741 ggcagcagaa tggcacccac gtggtcgacg gtcatgcata tgatggcgat gagcttgagg
     1801 gcgtcagact gttcgagcgt gaattgcccc ttctctgccg agagggtatc cgcgtcgccg
     1861 cttgtcattg tcaacgatcc gtcgcctcgc tctccttgac agcacccgac gcagcactaa
     1921 aatactgcag ctaagccgta tattggggcg tcgtctaatg gtaggacagt ggcctttgga
     1981 gccatgaatt gctggttcga ctccagccgc ctcagccaac ctcttcagac acagatatgc
     2041 ctcgatcctg ggttgacgcg gtcgaatcag cacagcctca ggacagaggc atcttaacca
     2101 gtgtagcaga ccctgctgtt ttttctagac cacctggccg gcgaggacac catacgtgct
     2161 tgccgcgtcg aatttgccca ttttctgcag cgccatcaca tatgctccga ggaccgggtc
     2221 gagcagtggc agctggagct gggcgagtgg cgcgacttcg tgcaccagca gctccagctt
     2281 gtcgacgaag acaggattgc cgcccccgaa gaccgagccc cccaggacaa catccacagg
     2341 ctcatgctcg aaacgcatct tgtggatcag cccgatgata ccctcagcga gtgactccgc
     2401 ctgcagtatc aggatgcgcc tgcacgcgtc gtcgccggcc gtggctccgt cgaagaccag
     2461 gggaaccaca gcgagtgccc ggtgaagcag ctctccatgc ccgaccatgt cctgcacggt
     2521 ctccaggaac atgagctgca aggcgtcata gtcgggtgcg ccggtcacct caagcaccgt
     2581 ctgctccagc acggacttcg gtccggtgcc gtcgtgggaa aggaacgcaa cgtgcaggat
     2641 gtccctcagc atgtccagac cgccgcctcg caggccgctc tcatagccga ggccgcggag
     2701 cgtgacccac gtcccgtcac ggctgcaggc gcacgcgttg gcgcctccac cacacacgag
     2761 agcaatgccc cagcccttgt ccgtccctgc tttcagcatg atccacgtgt cgttgaccag
     2821 gtcgaagatg agaccaggga acgcggctgt cagtgccgaa tcgagcgcct cccggtgcat
     2881 gggaaggtcc atgccggaca gcccaaacac tgcgcgctca acctgcgcta tcgacagccc
     2941 cgcagcggtc agagcgttct ggacagcctc tgcgacgcca gacattgcgg cctcgagacc
     3001 cacgacctgg tagttcgagg ctcctccctc gccggacccg gcgacatggc cgacatcatc
     3061 tccgacgaca gccttcgtcc tggtcgtccc cccatcaaca ccgaggtaat acgtcacggc
     3121 catatcagat gttctccgag tcgacgctgg tgtggaggtg gagcggcgtg aggaccttgt
     3181 atgcttctgc cagctgcatg ccgcgtcctt cacccagttc ggcggccttg aggttgagat
     3241 actgggtcgc catttccagg gtatccggcg tgaactggct cttgaacagg ctgagggccg
     3301 ccagcctcgt ctcgttctcg gctgagacgt cgacaaactg gtttggcctg ccggtaagga
     3361 agaacgcgat gaaacggacg tcgtgcgggg gcagctcggg gagatacagt ggcaggctgc
     3421 agtagatcag gctctccgcg gcggaccacc cacagttcag gtggtcctga tgggcctcgt
     3481 atgccagcca cgggtcgggc gccaggacgg cgtccggacg ctcctggcgg atgatgcgga
     3541 ccatggccgc tgtggtctcg ccgcagtgcg gcagttcacc gtcatggaag ggcaggtaca
     3601 ggtgcctggt tacgcccatg gcctcgccgg ctttctgatc ctccgcacgg cggatctcga
     3661 ccaggtgttc tcgtgtcatc gagacgtcta tgcttcccag gcagccgtcg gtcatggtga
     3721 ccagcgtgac gtccttcccc gccctggtta gtttgagcag aagcccgccc gcaaaccaga
     3781 ctgcgtcgtc ggagtgcggc tggaacacga cgtacttctg tgctgaatcg ataggtggga
     3841 ccggcaggaa ctcattccag ttcattgtcg acgacctccg ttcaggccgc aggcgtgccg
     3901 atgaaaggag tccctggtcc aggggattcg gggcacgatg aagtgctgag ggcgcactgc
     3961 gccttgacgt cctccagttt cttgccatgc agcgggtaca agaagacgaa gatgagcccg
     4021 atggcgatga gcagggccgg gacgacactt atcaggagcc tgaagccgag gacggcggtt
     4081 ggcgccatga gcgtgggatc tgtcacgggc ttgtacctcg tgatcgtctg aacgaacgag
     4141 atcgtgcctg ccgagatggc gacgctcatg cggatgatga gcgcgttgac accgtag
//
LOCUS       DATMZD010000199         2400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_56083,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000199 DATMZD010000000
VERSION     DATMZD010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56083"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..109
                     /locus_tag="VN478_04125"
     CDS             5..109
                     /locus_tag="VN478_04125"
                     /inference="COORDINATES: protein motif:HMM:NF013038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21895.1"
                     /translation="MTVDELAGLMGTAAYEVFTGLSKRVPRLYLDRHA"
     gene            complement(171..686)
                     /locus_tag="VN478_04130"
     CDS             complement(171..686)
                     /locus_tag="VN478_04130"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HWQ21896.1"
                     /translation="MRLLTHIVLHHSATDATLGMSDPEGAALWAAIQRNHQRHWAGTV
                     PGYVCDYHFGVGPTGVVLTGQPLAMVAFNCGNARINAVSVAVCFLGNFQLERMPAAQY
                     TAGLDLLRALLTKHDIPLSRVILHREVPDERTGIPGFTACPGRWFPSVAMRNALQQHG
                     GGSSSRACMTR"
     gene            complement(686..856)
                     /locus_tag="VN478_04135"
     CDS             complement(686..856)
                     /locus_tag="VN478_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016207517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvrJ family protein"
                     /protein_id="HWQ21897.1"
                     /translation="MDGLVTLIGNIGFPIAVSVYLLTVFGGKLDRMQTSLDRLADVIE
                     TSLRINGSAVTP"
     gene            complement(953..1723)
                     /locus_tag="VN478_04140"
     CDS             complement(953..1723)
                     /locus_tag="VN478_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015946666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HWQ21898.1"
                     /translation="MSILSAITALVAAPSGAATGFVLRFPLSATVNAAAVVIGSVLGM
                     LAGKFLKEDTRTAIFIANGLATLSIGIGMALKTGNAVIMVMAIVSGVVVGELLRLEDR
                     VNAAAERFQKRLHVSSGSLWAEGLVTATLIYCIGPMTIIGSIQEGMTGNGTLIYTKSI
                     LDLVISISLAAALGSGVAFSAIPVFIIQGSLTVLGSALGFLMQPYILAELTATGGLMV
                     IAIGINLLGLKKIKLANLLPGLIFAVLYALLFHALKLV"
     gene            complement(1742..2350)
                     /locus_tag="VN478_04145"
     CDS             complement(1742..2350)
                     /locus_tag="VN478_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21899.1"
                     /translation="MLIQVAINNIESVEQGEEILKKWHYHLDEDQSHVVMEREVDGAT
                     VRISGFHPWADELDEDGEECSCGGDDDNCECGGDHCRDGHERVEGESCCCHSEDEAAE
                     VKKSDDDEEGDEEGEESEVIYLGQALFVPADEQAHTLATELMDDDYIGTISVYMAGAE
                     LTGTDAFRGLPAGTKDAVIAAIEELGDAEAYLSMYEVMGLKD"
BASE COUNT          481 a          735 c          716 g          468 t
ORIGIN      
        1 gtccattaca gtcgatgaac tggcaggact gatggggacc gccgcatacg aggtcttcac
       61 cgggctgtcc aagcgcgttc cccggctcta cctggatcga cacgcctgaa cctgcgtatc
      121 aggacgtaac agagccccgc ccaaaggcgg ggctctgtcg tactctctgc ctaccgcgtc
      181 atgcaagccc gggatgagga gcctcctccg tgttgttgga gcgcgttccg catggcaacg
      241 gagggaaacc accgtcccgg gcacgcggtg aagccgggga tacccgtcct ctcgtcgggg
      301 acttcgcgat ggagaatcac gcgtgagagc gggatgtcat gcttcgtcaa gagagcgcgc
      361 agcagatcta ggccggccgt gtactgcgca gccggcatgc gttccagctg gaagttgccc
      421 aggaagcaca cggcaacact cacagcattg atccgtgcgt tgccacagtt gaatgccacc
      481 atggcaagcg gttgcccggt gaggacaacg ccggtgggtc cgaccccgaa atgatagtca
      541 cacacgtagc cggggacggt tcctgcccag tgccgctgat gattgcgctg gattgcagcc
      601 cacagtgcgg caccctctgg atcgctcatc ccgagagtcg catccgtcgc gctgtggtgc
      661 aggacaatgt gggtgagcag ccgcatcagg gcgtgaccgc cgaaccgttg atgcgcaggg
      721 acgtctcgat gacgtccgcc aaccggtcca ggctcgtctg catccggtcc agcttgcctc
      781 cgaatactgt cagcaggtac actgagacgg cgatgggaaa accgatgttg ccgataagcg
      841 tgacaagtcc gtccatgtcg tgcctccttg gcaggggttg cctgcactcc tgttatggag
      901 cggcatacat ggcggacggg cccgacgtgt gtccctgacg ggacgaacga cgtcagacga
      961 gtttcagagc gtggaagaga agggcgtaca ggacggcgaa gatcagtccc ggaaggaggt
     1021 tggccagctt gatcttcttc aggcccagca ggttgatgcc gatggcgatg accatgagac
     1081 cgcctgtcgc agtcagctcg gccaggatgt agggctgcat gaggaacccc agggcggagc
     1141 cgagcacggt caagctcccc tgaatgatga agaccgggat cgcgctgaac gccacgccgc
     1201 tgcccagcgc cgcagcgagc gatatgctga tgaccaggtc gaggatggac ttcgtgtaga
     1261 tgagcgtgcc attgcccgtc atcccctcct ggatggaacc aataatggtc atggggccga
     1321 tgcagtagat gagggtggca gtcacaagcc cctcagccca caggctgccg gaagacacgt
     1381 ggagccgctt ctggaagcgc tcggccgccg cgttgaccct gtcttcgagc cgcaggagct
     1441 ctcccacgac gacgccactt acgatggcca tgaccatgat gacggcgttg cccgtcttga
     1501 gggccatacc gatccctatg gaaagcgtcg ccagcccatt cgcgatgaag atggcagtac
     1561 gggtgtcctc cttgaggaac ttcccggcca gcatgccgag cacgctgccg atcacaactg
     1621 cagcggcgtt gactgtcgca ctcaatggga atctcaggac aaaacccgtt gccgctcccg
     1681 aaggagcggc aaccaacgcg gtaattgccg aaagaatgct catgaacagg acttgtctct
     1741 cctagtcttt gagtcccatg acctcgtaca tcgagagata ggcttcggcg tcgccgagtt
     1801 cctctatcgc agcaataacg gcatccttgg tgcccgcggg cagtccgcgg aatgcatccg
     1861 tcccagtgag ctcagcaccc gccatgtaga ccgagatggt accaatgtag tcgtcgtcca
     1921 tcagttcggt ggccagagta tgggcctgct catcggcagg cacaaacaga gcctggccaa
     1981 gatagatgac ctcggactcc tcgccttcct cgtccccttc ttcgtcgtcg tcagacttct
     2041 tgacctctgc agcctcgtct tcgctgtgac agcagcacga ttcgccttcg acgcgttcat
     2101 ggccgtccct gcaatgatcg ccaccgcact cacagttatc gtcgtcacca ccgcaggagc
     2161 attcttcgcc atcctcgtcc agctcgtcgg cccacggatg gaacccggag atccgtaccg
     2221 tggcaccgtc gacttcgcgc tccatgacca cgtgagactg atcctcatcg aggtggtagt
     2281 gccacttttt cagaatctct tcgccttgct caacgctttc gatgttgttg atagccacct
     2341 ggatcagcat ggggaacctc caatgagata gtatgggatt cattgtacac gtgtttgtgt
//
LOCUS       DATMZD010000200         3221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_86365,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000200 DATMZD010000000
VERSION     DATMZD010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86365"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /gene="pstA"
                     /locus_tag="VN478_04150"
     CDS             <1..613
                     /gene="pstA"
                     /locus_tag="VN478_04150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HWQ21900.1"
                     /translation="AIGTPFGMFAGIYVSEHRGQRFADVVRLCADALQGVPSIVIGIF
                     GYTFLVARFRSYSALSASIALAFMFVPTTARVTEEVLNLVPSGLKEAAYALGARRSSV
                     VWNVSIKTAAAGITSGLLTSMARIAGETAPLLFTAFGSRYIEWNMLRPMSALPLQIYI
                     YASSPYPVLQQQAWSAAFVLVLLILLLQYGAKHLIARVLRVKA"
     gene            625..1389
                     /locus_tag="VN478_04155"
     CDS             625..1389
                     /locus_tag="VN478_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020156001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HWQ21901.1"
                     /translation="MEEPVIVLETQKINAWFGKDQVLFDVSVRIPKKRVTAIMGPSGC
                     GKTTFVRTLNGLHLLTPGARSSGTVLLEGVDTSTMDIVDVRKHIGMVFQRPNPFPTMT
                     VYENVLAGYRLTGQSLTREKADHIVETTLNKVGLWTEVQAKLFKAGTSLSGGQQQRLC
                     IARTIAMEPAVVLFDEPTSALDPKSTAIIEELLSDLKADYSILFVTHGLQQAARISDY
                     VAFFMTGELVEFGETKRVFVGPQDKRTEDYLSGKYG"
     gene            1407..2084
                     /gene="phoU"
                     /locus_tag="VN478_04160"
     CDS             1407..2084
                     /gene="phoU"
                     /locus_tag="VN478_04160"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HWQ21902.1"
                     /translation="MHEEKLQEIKDKLFAFSALASSMVEKGTRAIVTKDEALAKEVID
                     VDEPVANDFDHSIERETINFIALYQPEARTLRSVYMYAKIGTDLERLADHGVKIAESA
                     QYLIARPDIKPYIDIPRMSSEARKMLADAMTAFSNQDTRLAIDVVQRDDIVDQLSNQI
                     LRELLTYMMADPSTIDRALQILFISGNLERVADLATNIAEDVVFATTGRLIRHNRLTK
                     EELDKLS"
     gene            2405..>3221
                     /gene="thrC"
                     /locus_tag="VN478_04165"
     CDS             2405..>3221
                     /gene="thrC"
                     /locus_tag="VN478_04165"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HWQ21903.1"
                     /translation="MNRQLTCVTCGSTYPLEEHRLRCDCGGLLDVHLDMDDAPSPATL
                     AATFESRLSSRLPRDLSGVWRYRELVLDLSAEETVTFPEGRTNLYAMDPEPLGLSALW
                     AKHEGENPTGSFKDRGMTVAVSWARHQGARALVCASTGNTSASLAAYGARAHLPVFVL
                     IPEGKISQGKLAQALAYGAHTLQIDGSFDDAMHAVEQMATTHDLTVVNSLNPFRLEGQ
                     KTIILDSYFQLGQMLPDWIVVPGGNLGNTSAFGKALIEMKALGLIDRVPRLAVI"
BASE COUNT          644 a         1070 c          931 g          576 t
ORIGIN      
        1 cgctattggt acgccattcg gcatgttcgc cggcatctac gtttcggagc accgcggtca
       61 gcgatttgcc gacgtcgtgc gcctgtgcgc cgacgcattg cagggggtgc cttccatcgt
      121 catcggcatc ttcggctaca cgttcctggt cgcgcgcttt cgcagctact ccgctctctc
      181 ggccagcatc gcgcttgcct tcatgttcgt gccgacgacg gcccgcgtca cggaggaggt
      241 cctcaacctg gtaccctcgg gcctcaagga agcggcatac gctcttggag ccagaaggtc
      301 cagcgtcgta tggaacgtta gcatcaagac cgccgcggcc ggcatcacgt cggggctgct
      361 cacgtccatg gcacgcatcg caggtgaaac cgcgcccttg ctgttcacag ccttcggtag
      421 tcggtacatc gaatggaaca tgctccgtcc gatgtccgcc cttccactcc agatctacat
      481 ctacgccagc agcccgtacc ccgtcctcca gcagcaggcc tggagtgccg cgtttgtgct
      541 ggtcctgctc atcctgctgc tccagtacgg cgccaaacat ctcatcgccc gcgtactgcg
      601 ggtgaaagca taaggaaggg acccatggaa gaacctgtca tcgttctcga aacccagaag
      661 atcaacgcct ggttcggcaa ggatcaggtc ctgttcgacg tctcggttcg cattccaaag
      721 aagagggtca ccgccatcat ggggccgtcc ggctgcggca agacgacgtt cgtccggacc
      781 ctgaacggac tgcacctgct gacaccggga gcccgttcgt ccggcaccgt cctgctggaa
      841 ggcgtcgaca cgtcgaccat ggacatcgta gacgtgcgca agcacatcgg catggtcttc
      901 cagagaccca accccttccc gacgatgacc gtctacgaaa acgtgctcgc cggctaccgg
      961 ctgactggtc agagcctcac gcgggagaag gccgaccaca tcgtggagac gacactgaac
     1021 aaggtaggct tgtggacgga ggtccaggcc aagctgttca aggccggcac gtccctttcc
     1081 ggagggcagc agcagcgcct gtgcatcgcc cgcacgatcg ccatggagcc ggcagtcgtg
     1141 ctgttcgacg agccgaccag cgccctcgac ccgaagtcga ccgccatcat cgaggaactg
     1201 ctgtctgacc tcaaggctga ctacagcatt cttttcgtga cccacggcct ccagcaggcg
     1261 gcacgtatct ctgactacgt cgccttcttc atgaccggtg aactagtgga gttcggcgag
     1321 acgaagcgcg tgttcgtcgg cccacaggac aagcggactg aagactacct gtccggcaag
     1381 tacggatgac agggaaggag tagaccatgc acgaagagaa gcttcaggag atcaaagaca
     1441 agctgtttgc gttcagcgcc ctggccagtt ccatggtgga gaagggtacg agggctatcg
     1501 tgacgaaaga cgaggctctc gcgaaagagg tcatcgacgt ggacgaaccc gtggcaaacg
     1561 acttcgacca ctccatcgaa cgcgagacca tcaacttcat cgcgctgtat cagcctgagg
     1621 cgcggaccct gcgcagcgtg tatatgtacg ccaagatcgg cacggatctc gagcgcctgg
     1681 ccgaccacgg cgtcaagatc gccgagagtg cgcagtacct gatcgcacga cccgacatca
     1741 agccctacat cgacatccct cgcatgagca gtgaagcgcg gaagatgctg gcagacgcaa
     1801 tgacagcgtt ctcgaaccag gatacccgtc ttgccatcga cgtcgtgcag cgtgacgaca
     1861 tcgtcgacca gctcagcaac cagatcctca gggagctcct cacctacatg atggctgacc
     1921 cgagcacgat cgaccgcgcc ctccagatcc tgttcatcag cggcaacctg gagcgcgtcg
     1981 cggacctggc gaccaacatc gccgaagacg tcgtctttgc caccaccggg cgcctcatcc
     2041 gccacaaccg cctcaccaaa gaggaactcg acaagctaag ctgagcatcc cgttgcccgc
     2101 cgcgaaggcg ggaacccatg cgtcagccag gactgggttc tcgcatgcgc aggaatgacg
     2161 tgcccggggc tcttcatctg tcgttcccgc cgagcctttc gcgaaggcgg gaacccatgc
     2221 gtcagccagg actggattcc cgcatgcgca ggaatgacgt gcccggggct cttcatctgt
     2281 cgttcccgcg aaggcgggaa cccatgcatc ggcctagagc aggattctcc aggacacgag
     2341 atgtagcatg ttacagtagt tgtcactggg ccgttctgca ccatactgga atcgaggtgt
     2401 gcccatgaac agacaactga cgtgtgtgac ctgtggatcg acctaccctc ttgaagagca
     2461 tcggctccgg tgcgactgtg gtggactgct cgacgttcac ctcgacatgg acgacgctcc
     2521 atctcccgcc accctcgctg cgacgttcga gtcgcgtctc tcgtctcgcc ttccccggga
     2581 cctctcgggc gtctggcgct accgcgagct cgtgctggac ttgtccgcag aggaaaccgt
     2641 caccttcccc gagggccgca ccaacctcta tgctatggac cccgagccac tcggcctgtc
     2701 cgccctctgg gcaaagcacg aaggcgagaa ccccacgggc tccttcaagg accgcgggat
     2761 gaccgtggcc gtgagctggg ccaggcacca gggagcccgc gccctcgtct gtgcctccac
     2821 cggcaatacc tcggcctcgc tggcggcgta cggcgcacgc gcccacctgc ccgtcttcgt
     2881 cctcatcccg gagggcaaga tctcacaagg caagctggcg caagcgctcg cctatggcgc
     2941 tcataccctg cagatcgacg gcagtttcga cgacgcgatg catgcagtgg aacagatggc
     3001 cacgacgcac gacctgacag tggtcaactc cctgaacccc ttccgcctgg agggacagaa
     3061 gactatcatc ctcgacagct acttccagct gggacagatg cttcccgact ggatcgtcgt
     3121 cccgggagga aacctgggca acaccagcgc gtttggcaag gcactcatcg agatgaaggc
     3181 gctgggcctc atcgaccgcg tgccgcgctt ggcagtcatc c
//
LOCUS       DATMZD010000201         2084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_75518,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000201 DATMZD010000000
VERSION     DATMZD010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75518"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="VN478_04170"
     CDS             complement(<1..211)
                     /locus_tag="VN478_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013322842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HWQ21904.1"
                     /translation="MPLDFKGDLNPEQLRAVEECKGPCLVLAGAGSGKTRVITYKLAY
                     LINQRGIAPSHIMAVTFTNKAAGEMR"
     gene            complement(211..807)
                     /locus_tag="VN478_04175"
     CDS             complement(211..807)
                     /locus_tag="VN478_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21905.1"
                     /translation="MGYFIDLLFLFVRRPAEGLRRIREHPSILAALIALLLTGVIDAM
                     SFRLMDIVGLLKSLGLASPMADAIMQAQQASATFWTLVTEPFFLVMLSVVVIDAVGQL
                     AWKRTAAPYLYTALSISGLIGAALRVCGILLAGVASGVLLDVCTYGAILYTLVMGVLA
                     VRLFYEKSTVKALLVYLLPTLLGVGILVLLTLTVGGAA"
     gene            1014..1457
                     /locus_tag="VN478_04180"
     CDS             1014..1457
                     /locus_tag="VN478_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21906.1"
                     /translation="MLFDTLFVISAAIIIARNLFSRQGKVGISVLAIGLSALLTASLT
                     PYYVSFIRVPNQGLATVAYYVVTYLLLYVLIAVPAVILSSVIKIVIAGLLLGLVTHLL
                     PVSISGTLVSESRVYPLMAPLFARLPPIDLQALGRWLHSLLPGSH"
     gene            1723..>2084
                     /locus_tag="VN478_04185"
     CDS             1723..>2084
                     /locus_tag="VN478_04185"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HWQ21907.1"
                     /translation="MDDKFAREGLTFDDVLLIPGKSDVLPRDVDVGTQLTRTIHLNIP
                     VVSSAMDTVTESTMATAMALEGGIGIIHKNMSIERQAAEVAAVKSTCVHSPSSVAHLD
                     PRQTVLEAHDMLERFNIFA"
BASE COUNT          430 a          644 c          599 g          411 t
ORIGIN      
        1 accgcatctc gcccgccgcc ttattggtga acgtgacggc catgatgtgc gacggggcaa
       61 tgccacgttg attgatgaga tacgccagct tgtacgtgat gaccctggtc ttgcccgatc
      121 ccgctcccgc aagaacaagg cagggacctt tgcactcctc gactgctctc agctgctccg
      181 ggttgaggtc ccctttgaag tcaaggggca tcaggctgcc ccaccgacgg tgagagtaag
      241 aagaaccaga atgccgactc cgagcagtgt cggcaggagg tagacgagaa gtgcctttac
      301 ggtgctcttc tcgtagaaga gccgaacagc aaggacgccc atgacgagcg tgtagaggat
      361 tgccccatag gtgcagacat ccagaaggac gccgctcgcg acaccagcca gcaggatccc
      421 gcacacacgc agggcagctc cgatcaaccc ggagatgctc agagcagtgt acaggtaggg
      481 ggcagcggta cgcttccatg ccaattgccc cacagcatcg atgacgacga cggacaacat
      541 gacgaggaag aagggctccg tgacgagtgt ccagaacgtg gctgaagcct gctgagcctg
      601 catgatggca tccgccatcg gactggcaag accgagggac ttcaggaggc cgacgatgtc
      661 catgagacgg aagctcatgg catcgatgac tcctgtcaac agcaaggcga tcaacgccgc
      721 gagtatcgac ggatgctcac gaatccgacg caggccctcg gcagggcgcc tcacaaacag
      781 gaatagcaga tcgatgaagt atcccacttc gttctccttt tcacggcagc gacgaccagc
      841 gtgcacagtc gacacggaca ctcacatgtc tgtcacggag ggcgcgtctc agtataccca
      901 ggaacgggcc ccagccaacg gcgaacggcg gcgccaggcg tcacaacttg ttgacgcaat
      961 gcgagccggg ttcatactac gctggtagaa gaagtctcgt tggaggtgtc ctgatgttgt
     1021 tcgacacgct cttcgtcata tctgcagcga tcatcatcgc ccggaacctg ttcagcaggc
     1081 agggcaaggt gggcatatcc gttctggcga tcggcctttc tgctctcctg acagcgtcgc
     1141 tgacgccgta ttatgtcagt ttcatccgtg tcccaaacca gggacttgcc accgtcgcct
     1201 actatgtcgt aacgtacctg ctcctgtatg tgctgatagc tgtcccagcc gtcatcctct
     1261 cgtcggtcat caagatcgtc atcgcaggtc ttctgctcgg cctcgtgacg cacctgctgc
     1321 ctgtttccat ctcaggcacc cttgtgagcg agagtcgggt ctacccgctg atggcacccc
     1381 tgtttgccag actccccccg atcgacctcc aggcgctggg aaggtggctc cacagcctgc
     1441 tgcctggcag tcactaggga ctcatgtcac gagcctcagc gggtcacgtc gcccggtgcg
     1501 tccggctcta tgcgggtgat aacgttgtga accatagcgc tcgctcgtcg atgccctcgc
     1561 tcactgacta ctgccccgtc cagggtcctg tatcgtgtgc tcggcttcca atgccccgac
     1621 tgtctcacag tccctcgttg acaaccggac cggcgttcgc taggctttag gtgtcgatac
     1681 gcgtgctgcg ggagccaagc ccgtctgcta ctgggaggaa cgatggatga caagttcgca
     1741 cgagagggtc tgaccttcga tgatgtcctg ctgatccctg gaaagtcgga cgtcctgcca
     1801 cgcgacgtcg atgtggggac gcagcttacg cgaacgatcc atctgaacat cccggtcgtc
     1861 agctcagcca tggacacggt caccgaatcc accatggcga cggcgatggc gctggagggc
     1921 ggcatcggca tcatccacaa gaacatgtcc atcgaacgcc aggctgctga agttgctgca
     1981 gtcaagtcga cctgcgtgca ctcgccttca tcggtagctc atctggatcc acggcaaacc
     2041 gtcctcgagg cgcatgatat gctcgaacgg ttcaacatct tcgc
//
LOCUS       DATMZD010000202         8390 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_146353,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000202 DATMZD010000000
VERSION     DATMZD010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8390
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146353"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2198)
                     /gene="leuS"
                     /locus_tag="VN478_04190"
     CDS             complement(<1..2198)
                     /gene="leuS"
                     /locus_tag="VN478_04190"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HWQ21908.1"
                     /translation="MREYDFHSIEPKWQDLWETGKAYRTEESSAKPKLFCLDYFPYPS
                     GDGLHVGHCRNYIPTDVISRFYRMKGFNVLHPMGWDAFGLPAENEAIRLKKNPREITL
                     KNTTNFKRQLKLLGTSYDWEREINSSNPEYYRWTQWFFLLLYKRGLAYEKESEQWWCP
                     SCKTVLANEQAKDGKCWRCGTPVEKKKLKQWFFKITDYADRLADDLDTVDWPERIKIM
                     QRNWIGKSVGAEVVFDIVSAGDGSRHPVTVFTTRLDTIMGATFLVLAPEHPLIHEIMS
                     GAQHVEVDAYAAASKTKTEIDRMSTDREKTGVFTGAYAVNPFSGEKVPVWIADYVLMG
                     YGTGAIMAVPAHDSRDFAFASRYGIPIRQVVGWSDVAYQATTWNDAYSEHGTLLNSGR
                     FDGLTTEDAFVAMVAEGETRGFAKAQTNYHMRDWLISRQRYWGAPIPMIHCPACGTVP
                     VPENQLPVVLPDVQGYEPSGTGESPLAGIKEWVETTCPVCGGPARRETDTMDGFACSS
                     WYELRYTSPHNDKAAFDRATVDYWLPVDLYVGGAEHAVMHLLYARFWTKVMYDEGLVG
                     FTEPFARLMNQGMLLSYDGQKMSKSKHNVITPDEVVEQYGADTLRLYELFMAPFEQEV
                     AWSKEGISGAHRFAKRLWELAQRTIEASRGTLTGVTVPKLELQINKLTRKITEDIEKF
                     KFNTAVAAFMEYFNFLAETVRNDETVLSATEWQAAMRRLLVVLAPFAPHMT"
     gene            complement(2260..3564)
                     /gene="aspS"
                     /locus_tag="VN478_04195"
     CDS             complement(2260..3564)
                     /gene="aspS"
                     /locus_tag="VN478_04195"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007072277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HWQ21909.1"
                     /translation="MERVLSSELSSHTGERIMMAGWMHRIRRLGSGMVFVHLRDRSGI
                     CQVIVESPEAVASIDGLLVESVIQVEGTVVAEPQATGGFELHDPSFVILSPVTEALPF
                     PINKPVINAHLDTFLDNAMVGLRAPSRQAIFKLSAGVQHGFRAALDARGFTEVATPKL
                     TGSATESGATVFPVEYFGKKAYLAQSPQQYKQMMVGVFERVYEVAPVFRAEPHQTVRH
                     LNEYVSMDAEMGFIKDHTSVMAVLTDVVHGILAYLSEHYAKELELLKVEMPLAPEAFP
                     HVYFPDAQQWIFEHHGEDCRGEPDLAPQHEKWLGEWAKETFQSDFLFVTGYPMVKRPF
                     YTAPNPEDPRYSNSFDLLFRGTELVTGGQRLNKFDDYARALESHGMTQEAVAGYLEAF
                     RFGMPPHGGFAIGLERFIMQLTGLTNLREAALFPRDMERLQP"
     gene            3775..6096
                     /locus_tag="VN478_04200"
     CDS             3775..6096
                     /locus_tag="VN478_04200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWQ21910.1"
                     /translation="MNQDSYTALIRSEMEFRALAGQAAVGITETETATGRYLFVNQRF
                     ADIVGYSCDELLTMSFHELTDPDDLAEDLGYMDRLVKGDLHEYSMEKRYRRRDGTPIW
                     VSLTVSPLWLEGESPRCVIGIVEDITERKRAEDALRESERKLREAQEMAHLGFWTWDV
                     KTRSVEWSEEVFKIFGLDPGEFTPQIDSILALSPWPEDNHRDTELIDHATKTHSRGFY
                     EQKFLRPDKSVGHYYSTFQGKYDENGELTTIVGTVLDITERKKAEEALQGSNELFSLF
                     IKHSPIYSYIKEVTPTESRVLQASDNYPEMIGVLGLHMAGKTMTELFPAEFAAKITAD
                     DWAVVSKGEILRLDEDLNGRNYTTIKFPIIRGDKTLLAGYTIDITDRKRAEEEKAALQ
                     AQLFQSQKVAAIGQLAGGIAHDFNNLLTGILGNVALMRSNLSPADPSLENLNAVETAA
                     RRAAELTKGLLTFSRSAMVLPEPMSIITALNTTLTLLKQSLPATIDIVCDYGQTAWNV
                     LLDQSQMTQILLNLAVNARDAMSGKGTLTIRAMNEVVGDEYVGRHPFARTGEFVHLSV
                     ADTGPGMSSEVMQHLFEPFYTTKPVGSGTGLGLSIVYGAVKQAGGWITAVSTEGAGTT
                     FDIYLPRCLKEPAEAAAPSPEPTDACSGTVLVVEDEPVVRMVAESFLSKSGYDVLTAA
                     DGSSALEVLRGHRGGVGLILLDMTMPGLTTSEIVQAIRVMDPAVPILLNSGYTTNDTV
                     RLMLDAGSVQGFLGKPYELHQLVARVRDLLKRG"
     gene            6313..7179
                     /locus_tag="VN478_04205"
     CDS             6313..7179
                     /locus_tag="VN478_04205"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWQ21911.1"
                     /translation="MALTDQTMKLADGRTLGYAEFGDPSGKPVLFFHGFPGSRLEAVC
                     LDTPARTVGIRLIAPDRPGFGLSDPKPGRLFSDWPEDVIQLAAHLRIYDFGILGTSGG
                     SPYVVACAERISERLTTAGIVSGMSPLHSDAVRLSMRPDQRRMFISVARFPWLARRMF
                     ARSMQEVQADFPSVLGHMSAERPDVDKIVLERQDVKEMLRANLTEAFRQGVAGAAQEL
                     ALYPRSWRLVMKNVDYPVRIWQGRKDVITPPSMAEELASLLPRSLTRWYADEGHSLLF
                     ARSEEILGQLVT"
     gene            7232..>8390
                     /locus_tag="VN478_04210"
     CDS             7232..>8390
                     /locus_tag="VN478_04210"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HWQ21912.1"
                     /translation="MLMGRGKRKITIVLLLSIGVLIAAGAGVWLGFFGPSVQSPQLAS
                     TLSDPRNQLRDFRSTDSLLVVAPHPDDECIATGGAIQKAIKAGARVRIVYLTYGDNYE
                     LAYWALKRLPALTPAEFKALGELRRAEAVKGAASLGLTPDHLTFLGYPDSGTLRIWET
                     TWKPGTSRLHVATDARSVPYADALNPGSPYNSAAITADLESVVRSFQPTVVLFPSPLD
                     LNPDHQSAYLFVTAALHDLDVHPSRYTYLVHQRRFPSPLLYNPLAMLTPPAFVASLPI
                     QLRNLGLTTGQETLKYASTMLYHSQIDVARRLLTSFSRQNEVYFEDQQIALSPADAPF
                     FLIPTDFLLERFGHRVGLRSMSATRPTPGTLRLLLSFPAAPTEDSLVELSVF"
BASE COUNT         1671 a         2585 c         2451 g         1683 t
ORIGIN      
        1 gtcatgtgcg gggcaaaagg cgccaggacc acgagcagcc gccgcatcgc tgcctgccac
       61 tcggtcgcgg acaacacggt ctcgtcattg cgcaccgtct cggccaggaa gttgaagtat
      121 tccatgaacg cggccacggc agtgttgaat ttgaacttct cgatgtcctc ggtgatcttc
      181 ctggtcagct tgttgatctg cagttccagc tttgggaccg tgacgcccgt gagcgttcca
      241 cgtgacgcct cgatcgtgcg ctgcgcaagc tcccacaaac gcttcgcgaa gcgatgcgcg
      301 cccgagatgc cttccttgga ccaggcgacc tcctgctcaa acggcgccat gaacagctca
      361 tacaggcgca gcgtgtccgc accgtactgc tcgacgactt cgtccggcgt aatgacgttg
      421 tgcttgctct tgctcatctt ctgaccgtcg tatgacaaca gcatcccctg gttcatgagg
      481 cgcgcaaagg gctctgtgaa gccaaccaag ccctcgtcgt acatgacctt cgtccagaag
      541 cgggcataca ggaggtgcat gacagcgtgt tcggcgccgc cgacgtacag gtcgacggga
      601 agccagtagt cgacggtcgc cctgtcgaag gccgccttgt cgttgtgggg actggtgtac
      661 cgcagctcat accacgagga acaggcgaag ccgtccatgg tgtcggtctc gcgccgggcc
      721 ggacccccgc acacggggca ggtcgtttcg acccactcct tgatcccggc aagaggtgat
      781 tcccctgtcc cgctcggctc atatccctgc acgtcgggca gcacgaccgg aagctggttc
      841 tccggaacgg gaaccgtccc acaggccggg cagtggatca tcgggatcgg ggcaccccag
      901 tagcgctgac ggctgatgag ccaatcacgc atatggtagt tggtctgagc ctttgcgaat
      961 ccccttgtct caccctctgc caccatcgcc acaaaggcgt cctcggtcgt caggccgtcg
     1021 aagcggcccg agttcagcag agtgccgtgc tcggagtatg catcgttcca ggtcgtggcc
     1081 tgatacgcga cgtccgacca cccaacgacc tgacggatcg ggatgccgta tctgctggca
     1141 aaggcaaagt cacggctgtc gtgagcgggc acagccatga tcgcgccggt accgtagccc
     1201 atgagcacat agtcagcaat ccacaccggg accttctcac cagagaatgg gttgaccgca
     1261 taggctcccg tgaagacgcc ggtcttctcc ctgtccgtcg acatgcggtc gatctcggtc
     1321 ttcgtcttgc tggccgcggc gtacgcatcg acttcgacat gctgggcgcc cgacatgatc
     1381 tcatggatca gcgggtgttc gggagcaagc acgaggaacg tggctcccat gatcgtgtcc
     1441 aggcgagtcg tgaagactgt cacagggtgt ctcgagccgt cgccggccga gacgatatcg
     1501 aaaacgacct cagccccgac cgacttgccg atccagttgc gctgcatgat cttgatgcgc
     1561 tcaggccagt ccaccgtatc gaggtcatct gccagacggt cggcgtagtc ggttatcttg
     1621 aagaaccact gcttcagctt cttcttctca acgggcgtac cacaccgcca acacttgccg
     1681 tccttcgcct gttcgttggc caggacagtc ttgcagctgg ggcaccacca ctgctccgac
     1741 tccttctcgt aggcaagacc ccgcttgtac agaagcagga agaaccactg ggtccaccgg
     1801 tagtattcag ggttggacga gttgatctcc cgttcccagt catatgaggt cccaaggagc
     1861 ttgagctgcc gcttgaagtt ggtggtgttc ttgagcgtga tctcacgggg attcttcttc
     1921 agcctgatgg cctcgttctc agctgggagc ccgaatgcat cccaccccat cgggtgcagt
     1981 acgttgaagc ccttcatgcg gtagaagcgg ctgatgacgt ccgtggggat gtagttgcgg
     2041 caatgaccga cgtgcagacc gtcgccggac ggatagggaa agtagtccag gcagaagagt
     2101 ttcggtttcg cagagctctc ttccgtccga taggccttcc cggtctccca gaggtcctgc
     2161 cacttcggtt caatgctgtg gaagtcgtat tctctcattg gtcactccaa ttgtgaatac
     2221 ctcaagtgcc tgaccccaac gatgttcaca tttgatggat cagggctgca gccgctccat
     2281 gtcgcgcggg aacaaggcgg cctcgcgaag gttcgtgagc cccgtcaact gcataatgaa
     2341 ccgttccagc ccgatggcaa acccaccgtg gggcggcatt ccaaagcgga acgcctccag
     2401 gtagcccgcc acggcctcct gtgtcatgcc gtgagactcc agagcccttg catagtcgtc
     2461 gaacttgttg agacgctgtc cgccggtgac gagctcagtc ccccggaaca gcaggtcgaa
     2521 cgagttggag taccttgggt cctccggatt cggcgccgtg tagaaggggc gcttcaccat
     2581 cggatacccg gtaacgaaga ggaaatcgga ttggaaggtc tcctttgccc actcgccaag
     2641 ccacttctcg tgctgaggag caaggtccgg ctctccgcgg cagtcctcgc catgatgttc
     2701 gaagatccat tgctgcgcgt cggggaagta gacgtgcggg aacgcctcag gcgccagcgg
     2761 catctcgacc ttgagaagtt cgagctcttt cgcatagtgc tctgagagat atgcaagaat
     2821 gccgtggacg acgtctgtca gaacagccat gacactcgtg tggtccttga tgaaccccat
     2881 ttcagcgtcc atgctgacgt actcgttgag atgacggact gtctggtgag gctcggccct
     2941 gaagacgggg gctacttcat acacgcgctc aaagacgccg accatcattt gcttgtactg
     3001 ttgagggctc tgcgccagat aggccttctt gccaaagtac tcgacaggaa agacggtggc
     3061 ccctgattca gtagcagacc cagtgagctt gggtgttgcg acctctgtga agcccctggc
     3121 atccaatgcg gcacggaatc cgtgctgtac acccgcgctg agcttgaaga tggcctgtct
     3181 ggatggcgct ctgaggccga ccatagcgtt gtcgagaaat gtgtccaggt gtgcgttgat
     3241 gaccggcttg ttgatcggga acggcagcgc ctcggtcact ggcgagagga tgacaaatga
     3301 gggatcatgg agctcaaacc cgccggtcgc ctgtggttca gcaacgactg tgccttcgac
     3361 ctggatgacg ctctctacga gcaatccgtc gatggaagcc accgcttcag ggctctcgac
     3421 tatgacctga cagatgccgg accggtcgcg gaggtgcaca aacaccatgc cgcttcccag
     3481 tctgcggatg cggtgcatcc accccgccat catgatgcgt tcgcctgtat gagaagatag
     3541 ttcgctgctc agcacacgtt ccatgtcaac ctccatatat gacaatggcc gctctcaggg
     3601 caaccgccca ggaccggccc tcgacaaccc ttatcaggtt acttcctgac tgtcttcctg
     3661 tcaacagcga actgctgatt gatcgacacg tatccttgat gccgccgggg ttgtgtctac
     3721 actatgcata gttctcgact gtcggagcca ggcacgtttc gcgtgaggtg accaatgaat
     3781 caggactcgt acactgcgct catacgcagc gagatggagt ttcgtgcgct cgccgggcag
     3841 gccgccgtcg gcataaccga gacagagact gcgaccggac gatatctctt tgtcaaccag
     3901 cgcttcgcgg acatcgtagg gtactcgtgt gatgagctgc tgacgatgag cttccacgaa
     3961 ctcaccgacc ctgatgatct cgcggaggac ctgggctaca tggaccgcct cgtcaagggt
     4021 gaccttcacg agtactccat ggagaagcga tatcgccgga gagacggcac gcccatctgg
     4081 gtgagtctca cggtttcacc actgtggctg gagggggagt ctccacgatg tgtcatcggt
     4141 atcgtagagg acatcaccga gcgcaaacgg gcagaagacg cactgcgcga aagcgagagg
     4201 aagttgcgag aagcccagga aatggcccat ctcggcttct ggacctggga cgtcaagacg
     4261 cggagcgtcg agtggtctga agaagtgttc aagatcttcg gcctcgaccc cggggagttc
     4321 actcctcaga tcgactcgat ccttgcgctg tcgccgtggc ccgaggacaa ccaccgtgac
     4381 acagagttaa tcgatcacgc gacgaagact cacagccgag gcttctacga acagaagttc
     4441 cttcgtccgg acaagagtgt gggacactac tactcgacct tccagggaaa gtacgacgag
     4501 aacggcgagc tgaccacgat tgtcggaacg gtgctcgata tcaccgaacg caagaaggcg
     4561 gaggaagccc tgcaggggag caatgaactg ttctcgctgt tcatcaagca ctcgcccatc
     4621 tactcctaca tcaaggaggt gacgcccact gagagtcgcg tacttcaggc cagtgacaac
     4681 tacccggaga tgatcggcgt cctgggtctg cacatggcgg gcaagacgat gaccgaactg
     4741 ttccccgccg agtttgctgc aaaaatcact gccgacgact gggccgtcgt aagcaaagga
     4801 gagatcctca ggttggatga ggacctgaat ggccgcaact acacgacaat caagttcccg
     4861 atcatccggg gagacaagac cctcctcgcc ggctacacca ttgacatcac cgacaggaaa
     4921 cgtgctgagg aagagaaggc tgcgctccag gcacagctgt tccagtcgca gaaggtggca
     4981 gccatcggtc aattggcagg cggcatcgct catgacttca acaacctcct gacgggtatc
     5041 ctgggcaacg tcgctctcat gcgcagcaac ttgtccccag ccgacccatc actggagaac
     5101 ctgaatgcag tcgagacagc ggctcgccgg gcagctgagc tcacgaaggg gctgctgacc
     5161 ttcagtcgca gtgccatggt ccttcccgaa ccgatgagca tcattacggc gctgaacacg
     5221 acgttgaccc tgctgaagca gtctttgccg gcaaccatcg acatcgtctg cgactacgga
     5281 cagaccgcat ggaacgtcct cctggatcaa tcgcagatga cccagatcct gctcaacctg
     5341 gcagtaaacg cacgggatgc catgagtggc aagggaacgc tgaccatccg cgccatgaac
     5401 gaggttgtag gagacgaata tgttggtaga catccatttg cgcgcacagg ggaattcgtg
     5461 catctcagcg tggccgacac gggccccgga atgtcatcag aggtcatgca gcatctcttc
     5521 gaacccttct atacgacaaa accagtgggt tcgggcactg gactgggact ctccatcgtc
     5581 tacggcgcgg tcaagcaagc tggtggctgg atcacggcag tatccaccga gggtgcgggg
     5641 acgactttcg acatctacct ccctcgatgc ctgaaagagc ccgcggaggc tgccgctcct
     5701 agccccgagc ccacagatgc atgcagcgga acagtcctgg tggtcgagga cgagccggtc
     5761 gtgcggatgg tcgccgagtc gttcctgagc aagagtggat atgatgtcct gacggcagca
     5821 gacggatcgt cagccctgga ggtcctgcga ggtcaccgtg ggggcgttgg actcatcctg
     5881 ctggacatga ccatgccagg cctgacgacc agtgagatcg ttcaggcgat ccgggtcatg
     5941 gatcctgcgg tgcccatcct tctgaactca ggatacacca ccaacgacac tgttcgactc
     6001 atgctcgatg cgggcagtgt ccaggggttc ctgggcaagc cctacgaact gcaccagctg
     6061 gtggcgaggg tgcgggatct gctgaaaagg ggctgagcgg ggtgctcgcc ctccccctac
     6121 ggtattccag ccaccatctg caaccaggtt caacgaggac gccagactca gccggtatgc
     6181 caggctctgg cgcgcgcagc aaacgtctga agcaggctgc gttcgccgta tcctggttga
     6241 ttggctgtcg cttcatggta aactcagctt ctggagaact gcagaagtga acttgagccg
     6301 ggtgaggcgc agatggcatt gactgaccag acgatgaagc ttgcggacgg acgtacgttg
     6361 gggtatgcag agttcggcga cccatcgggg aaacccgttc tcttctttca tgggtttccg
     6421 ggatcccgcc tggaggccgt ctgcctggac acacccgcgc gcaccgtcgg tattcggctc
     6481 atcgcgccgg acaggcccgg ttttggcctc tcggacccca aacccggacg tctgttctca
     6541 gactggccgg aggatgtcat acagcttgcc gcgcatctgc gcatctacga cttcgggatt
     6601 ctcggcacat ccggaggaag cccctacgtc gtcgcctgcg ccgagcgcat cagtgagagg
     6661 ctgaccactg caggaatcgt ctcgggcatg tccccgcttc acagtgacgc ggtccgcctg
     6721 agcatgagac ctgatcagcg gcgtatgttc atctcggtgg cacgattccc ctggcttgcc
     6781 cgccgcatgt tcgcccgaag catgcaggag gtgcaggctg atttcccatc ggtcctggga
     6841 cacatgtcgg ctgaacgacc cgatgtggac aagatcgtgc tcgaacgaca ggacgtcaag
     6901 gagatgctac gagccaacct gaccgaagcg ttcagacaag gcgtcgcagg cgctgcacag
     6961 gaactggctc tctatcctcg ctcctggcgt ctcgtgatga agaatgtcga ctatcctgtc
     7021 cgcatatggc aggggcgcaa ggatgtcatc actcctccat cgatggccga ggaactcgcg
     7081 tctcttcttc cccgcagcct gacacggtgg tacgccgacg aggggcactc cctgctgttc
     7141 gcccgttcag aggagatcct ggggcaactc gttacctgac aggcaactcc atgacagtga
     7201 ccgccagtga gtccgccgga ggaagcccgt gatgctcatg ggacgcggga agcgcaagat
     7261 cacgatcgtg ctgctgcttt ctatcggtgt actgatcgcg gcaggagcgg gcgtctggct
     7321 ggggttcttt ggtccatctg tccagtcccc gcagcttgca tccacactgt ctgacccgag
     7381 gaaccagctc agggacttcc gatcgactga ctccctgctt gtcgtggctc cccatcccga
     7441 cgacgaatgc atcgccactg gcggagcgat ccagaaggcc atcaaggccg gcgccagggt
     7501 acgcatcgtg taccttacat atggagacaa ctacgagctc gcctattggg cgttgaagag
     7561 actgccggca ctgacgccgg ccgagttcaa ggcactgggg gaactgcgcc gcgcggaggc
     7621 cgtcaaggga gctgcctctc tcgggttgac gcccgatcat ctaaccttcc tcgggtatcc
     7681 cgactcggga actctgcgca tctgggaaac cacctggaag ccgggcacga gcaggcttca
     7741 tgtcgctacc gacgcccgat cggtacccta tgccgacgcc ctgaacccgg ggtctccgta
     7801 caactctgcc gcgatcaccg ctgacctcga gtcagtggtg cgctcgttcc agcccacggt
     7861 cgtgctgttc ccttcgccgc ttgacctcaa ccccgaccat cagtcggctt atctctttgt
     7921 caccgcggcc ttgcacgacc tcgacgtgca cccgtcccgc tacacctacc tcgtccacca
     7981 gcgacgcttc ccatctccgc tcctgtacaa cccgctggcc atgctgactc cgcctgcatt
     8041 tgtggcttcg cttccgatcc agttgcggaa tctggggctg acgacgggtc aggagacctt
     8101 gaagtatgcg tcaaccatgc tctaccacag ccagatcgac gtcgcccgcc gcttgctgac
     8161 cagcttctca cgccagaacg aggtctactt cgaggaccag cagatcgctc tctctcctgc
     8221 cgacgctcca ttcttcctga tccccacgga cttcctgctg gaacggtttg gccacagggt
     8281 cgggctgcgg tctatgtctg ccaccagacc gactcccgga acgctgcgcc tgctgctatc
     8341 ctttcccgcg gctccgactg aggactctct cgtcgaactc tccgtgtttc
//
LOCUS       DATMZD010000203         6821 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_146391,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000203 DATMZD010000000
VERSION     DATMZD010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6821
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146391"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="VN478_04215"
     CDS             complement(<1..703)
                     /locus_tag="VN478_04215"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HWQ21913.1"
                     /translation="MELRGTLKDFSLEAILGLIRSGRKTGTLDLMVTTPARVPRRVSL
                     SFLGGEITLIQCGPLRGLDALREAAICAEGSFEFGVDSSLSPEDETVPVDMEVALATI
                     DEARNAMKSMSTSVPTTGVTFNHAVPVEETIHISLEEFRLLAVMHDGMTLNDLIAANT
                     APTVDTMRIVRQLMERGLLVTGVAVATVDQMAYEGVVALAEYVGGQAGVTIFNQYFRP
                     GAPVAEWTKAAPAFRS"
     gene            1142..2167
                     /locus_tag="VN478_04220"
     CDS             1142..2167
                     /locus_tag="VN478_04220"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HWQ21914.1"
                     /translation="MAQDDGELIRLALEQLPDGVVVADAHDRVVALNAQARLLRGVRP
                     DTSIAFPLLFGGSVTDDIEQGLGALRTGTATEFSVVEGDPSTGKVYEHRCVPVKTLAG
                     EYAGTAVTSHDVTDRQLRDARQEAKVSGLQRQVAVLQDALQERFVDGMITLVDALEAR
                     DRYTRGHSTRVALMSGKVARRVLSHAADAAEIELAARLHDIGKVAVPDRLLDKPTSLT
                     PDEIVLMDTHPLVGEDILRPISQLRNVALIIRNHHERWDGAGYPDGLEGDHIPIGSRI
                     LALADSFDAMTSQRPYRSSLSVPEARDEIVRQIGTQFDPAIASVFLDMIGTGEIVAET
                     QTYGEAG"
     gene            2164..2883
                     /locus_tag="VN478_04225"
     CDS             2164..2883
                     /locus_tag="VN478_04225"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HWQ21915.1"
                     /translation="MIRRIASQRRHLDASRGSEDFLLFSYGDYFEPGNVRWGGLRFFN
                     DDLIHPAGGFPLHFHDEIEVVTIVVAGEIRQRQGALPPVRLRAGDVQVLTSGTGVHHT
                     EINECPGQAHVYQLGIFPRVKGVAPGHAEAAFGVAPASNEIVAVASGQNKEGAIPMNA
                     DATVFVGSLDAYQMIDYHMAADRCALLYLSRGTLTVNGVDYESGDQARIADEPQFMAG
                     AIDHSDFILVDVPTLGQMVRE"
     gene            complement(3011..4327)
                     /locus_tag="VN478_04230"
     CDS             complement(3011..4327)
                     /locus_tag="VN478_04230"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWQ21916.1"
                     /translation="MTFDSFHLDPRLTTGIVSCGYTTPTPIQEQAIPPVMEGRDVMGL
                     AQTGTGKTAAYALPIMERLLTGPRGSLRALVVAPTRELAEQIADDFRDLGRKARLTVL
                     TIYGGVGINPQIDALRRGVDIVVACPGRLLDHITQGTVDLTKVQVLVLDEADMMLDMG
                     FLPDVRRIIKNLPAERQNLLFSATMPVDIRHLADAILRDPVTVQIGHSAPVETVSHAL
                     YPVPQHLKTPLLIELLKHTDTESVLIFTRTKHRAKKLGEKLAKEGYRATSLQGNLSQR
                     ARQAALGGFRDGSYKVLVATDIAARGIDVTQISHVINYDIPDTTDAYTHRIGRTGRAL
                     HSGDAFTLVTEEDTMLVRDIEKVLGTPIERRTLTGFDYKAPAPKRETEFAWDPRSGAG
                     SSRPQHSSSRDGQSASAPRHHENSVNTPKTSNHRSETRRQASIRTS"
     gene            complement(4620..6236)
                     /locus_tag="VN478_04235"
     CDS             complement(4620..6236)
                     /locus_tag="VN478_04235"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWQ21917.1"
                     /translation="MQITCTDLSFSYPEQATLLFDGVTFSISDSSRLGIFGPNGSGKS
                     TLLGLLAGRLQPTTGTIGRSRPTPRIGLIEVDAGDIRSVLASAIGVRDPALGAMWSAM
                     ESGPAADSAGEAAAEFAAQGGYAVLAEVQHTLADAGLPAALWDHTVKSLSVGERLWLR
                     VAEALIARADLLVLDEPTSHLDIQKRAELAAVLQDLDRPYVVVSHDRRFLDLVCTQVL
                     ELTRGHGRLYSGGYTQYIDTVRAQEEHDRDVYAVQTRKVRQLKRAIGRVKGQAGGIEA
                     LAYASSSGFFSHKAAKMEKRAKAMRTQLERSLAEATAAKPFIEKKRDYVLEGSTRGGI
                     LVSLVRVTASAGDRTLFHDLSLTVRSGEHWCLLGPNGAGKSTLLQIMTGMRQPDGGDV
                     TLSPSVRVGFVPQQIVLDHGEQLPVDLVREAGGIGSEDARTLLGTLGIEDDQVFRPVG
                     LLSAGQQKRVFIARLVAARPDLLVIDELEGNLAIDALALLEWALSRFTGALVMVTHDA
                     TLAHAVGQQFISLDGAGGWSQENRFDEGDP"
     gene            complement(6406..>6821)
                     /locus_tag="VN478_04240"
     CDS             complement(6406..>6821)
                     /locus_tag="VN478_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HWQ21918.1"
                     /translation="IGSTHAFGTYADALERLEACVRPGGWLLVADCYWKQHPSPEYLS
                     FMECEESVYGTHEGNIGIAEKLGLIPLWASVANDDEWDEYEWRYHMNVENFAHDNPDD
                     PDHDAMLERIRSWSHAYLAWGRATLGFGLYLFRNP"
BASE COUNT         1227 a         2029 c         2190 g         1375 t
ORIGIN      
        1 ctgaccggaa ggcaggtgct gccttggtcc attcggccac gggagctccg ggccggaagt
       61 actggttgaa gatggtcacg cctgcctgtc cgccgacata ctcggcaagg gcaaccacac
      121 cctcatatgc catctggtcg accgttgcca cggcgacgcc ggtgacaaga agcccccgtt
      181 ccatgagctg tcggacgatc cgcatggtgt cgacagtagg tgcagtgttc gcagcgatga
      241 gatcgttgag tgtcatgccg tcgtgcatga cggccagcag ccggaattcc tctagactga
      301 tgtggatagt ctcctcgaca gggacggcgt ggttgaacgt gacgccagtc gtggggacgc
      361 tcgtgctcat cgacttcatg gcgttgcgtg cctcgtcgat tgtcgccagc gcgacttcca
      421 tgtcaaccgg gacggtctcg tcttcgggcg agagcgaact gtcgacgccg aattcgaatg
      481 acccttccgc gcatattgcc gcctcgcgca gcgcatcgag cccgcgcagg gggccacact
      541 ggatcagcgt tatctccccg cccagaaagg aaaggctgac gcggcgtggc actctcgccg
      601 gcgtggtaac cataaggtcc agtgtgccgg tcttgcgacc gctgcgaatc aggccaagaa
      661 tggcctcgag agaaaagtcc ttgagtgttc cacgtagctc catttcgtgc tcccctggat
      721 cgccggtgga catctgcatc agcgtcaacc acaaatactg agcaggcctg tgacttcatg
      781 gtatgcgacc ttggcgcttt tgcaacagtg acggcggccg gggtacggcg aacgccgcat
      841 ctctactgcc ccgtgtgcct gcgtgctcct gcagccgatt gtgaacggtt gcgagcatga
      901 cctggtcttg tttgtccgga tgggcctgct acactaacat atgtgttaga tgagaaggtt
      961 tcgactttct ttctccaacc aacttcctac aatggcgaga ggcgcaagcc tttcgccttt
     1021 ttcattttcc atcggcagtg actctcccga gctgccggcc cccgctttcc ctgcgcacgt
     1081 ttacactgac aagccacagc gctacactga tagcaatgtg gggtgacctc tggaggagcg
     1141 tatggcacag gatgatggcg aactgatacg tctggcgctt gaacagcttc ctgacggcgt
     1201 tgtagtggcc gacgctcacg accgtgtcgt agccctcaac gcgcaggcac gcctgctgag
     1261 gggtgtgcgg ccggatacga gtatcgcgtt tcccctgttg ttcggtggct ccgtgacaga
     1321 cgacatcgaa cagggattgg gagcattgcg gacaggcaca gcgacggagt tctcggttgt
     1381 cgagggtgac ccctcgacgg gcaaggtgta tgaacatcgg tgtgtaccgg tgaagacgct
     1441 tgcaggcgaa tacgccggaa ctgccgtgac gtcgcacgat gtcaccgatc ggcaacttcg
     1501 ggacgctcgt caggaagcga aagtctcggg gcttcagcgg caggtcgccg ttctccagga
     1561 tgcactgcag gagcgcttcg tcgacgggat gatcacgctt gtcgatgcat tggaggctcg
     1621 agaccggtac acgcgcggcc attccacccg ggtcgccctg atgtccggaa aggttgcccg
     1681 aagggtcctg agccatgccg ccgatgctgc tgagatcgag ttggccgccc gtcttcacga
     1741 cattggcaag gtggcggttc cggaccgcct gctggacaag ccgaccagcc tgacaccgga
     1801 tgagatcgta ctcatggaca cgcatcccct cgtcggagaa gacatcctgc gccccatctc
     1861 acaactgcgc aatgttgcac tcatcatccg caatcaccat gagcgatggg atggggcggg
     1921 ctacccggat ggtctggagg gagaccacat ccccatcggt tctcgaatcc ttgcactcgc
     1981 ggacagcttc gatgccatga cgtcacagcg tccctaccgc agctctctct cggtgccgga
     2041 ggcccgcgac gagatcgtgc gacagattgg gacacagttc gatccagcga tcgcgtcggt
     2101 gtttctggac atgatcggta ccggcgagat cgtggccgag acccagacct atggtgaagc
     2161 tggatgatcc gccgtatcgc ttcgcagcgg cgacatctcg acgcgtcgcg gggttccgag
     2221 gacttcctgc tgttctcata cggcgactac ttcgagccgg ggaacgttcg atgggggggt
     2281 ctccgtttct tcaacgatga cctgatccat cctgccggtg gctttccgct gcacttccat
     2341 gacgagatcg aggtcgttac catcgtcgtg gccggggaga tccgacagcg gcagggggct
     2401 ttgccaccgg tacgtcttcg cgcgggagac gtccaggtcc tgacgtcggg aacgggcgtt
     2461 caccacaccg agatcaacga gtgtccgggg caggcgcacg tgtaccagct ggggatcttt
     2521 ccccgggtca agggagtggc gcccgggcat gctgaggctg catttggagt agcccctgct
     2581 tccaacgaga tcgttgccgt cgcgtcgggt cagaacaagg aaggggcgat cccgatgaat
     2641 gctgacgcga ccgttttcgt cggctcgctc gacgcctacc agatgatcga ctaccacatg
     2701 gcagcggacc gttgtgcact cctctatctg tcccgcggta ctctcactgt caatggagtc
     2761 gactacgaat caggtgacca ggcgcgcata gcggatgagc cccagttcat ggcgggggcg
     2821 atcgatcact cggatttcat ccttgtggac gtgccgactc tggggcagat ggttcgggaa
     2881 tagcgtaccc gaacagggac aggaagtctg gaaaaagcga ggggccccag acggggcccc
     2941 tgcgaaggag ggcatgtgat aacgtttggc gctatcacgc cctcattata tcacatctgt
     3001 gcaaacgctg tcaagaggtc cggatactgg cctgtcttct tgtctctgac cgatgattcg
     3061 aggtctttgg cgtgttcacg ctgttttcat gatgtctcgg cgctgaagca gactggccgt
     3121 cacggctgga tgaatgctgg gggcggctgg aaccagcacc ggatcttgga tcccatgcga
     3181 attcggtttc acgcttcggg gccggggcct tgtagtcgaa gcccgtcagt gtacgccgtt
     3241 cgataggcgt tcccaggacc ttctcgatgt ctcggaccag catcgtgtct tcctcggtga
     3301 cgagagtgaa cgcatctccc gagtggagag cgcggccggt acgcccgatg cgatgggtgt
     3361 acgcgtccgt ggtgtcgggg atgtcgtagt tgatgacgtg tgagatctgt gttacgtcga
     3421 tgccacgggc tgcgatgtcg gtggcgacga gtaccttgta gctgccgtca cggaagccgc
     3481 ccagcgctgc ctggcgcgca cgctgtgaga ggttgccctg cagcgacgtc gctctgtagc
     3541 cctccttggc cagcttctcg ccaagcttct tcgcacggtg ctttgtgcgg gtgaagatga
     3601 ggaccgactc ggtgtcggtg tgcttgagca gctcgatgag gaggggcgtc ttgagatgct
     3661 gcggtaccgg gtacagggca tgcgagacgg tctcgacggg agcggagtgc ccgatctgga
     3721 cggtcacggg gtcacgcagg atggcgtcgg ccagatggcg gatgtcgacc ggcatggtgg
     3781 cgctgaacag caggttctgg cgctcagccg ggaggttctt gatgatgcgg cgtacatcgg
     3841 gcaggaagcc catgtccagc atcatgtccg cttcgtcgag gacgaggacc tggaccttcg
     3901 tgaggtcgac cgttccctgg gtgatgtggt cgagcagacg gccggggcag gccacgacaa
     3961 tgtccacgcc gcgccggagc gcgtcgatct gagggttgat gcccacgccg ccgtagatgg
     4021 tgaggacggt gaggcgagcc ttgcggccca ggtcgcggaa gtcgtcggcg atctgctccg
     4081 ccagttcacg ggtcggggcg acgacaagag cgcgcagcga accgcgtggt cccgtcagca
     4141 ggcgctccat aataggaagc gcataggctg ccgtcttgcc ggtgccggtc tgggcaaggc
     4201 ccatgacgtc acgtccttcc atgacaggtg gaatagcctg ctcctggatg ggtgtgggtg
     4261 tggtgtagcc gcatgaaacg atacccgtgg tgaggcgggg gtcaagatga aatgaatcaa
     4321 atgtcactgg agctccttgt gccggcgctc gacaggatga cgctctcaga cagtcagcag
     4381 gcggctcttt catatgtcgg atgtcgccgc acgggtgtgg cgagtgctat tggacccatg
     4441 ctttgagaca tcgacagtaa gcgttcgatc ggattcctcc ggtcgtctgt ttccctgtgc
     4501 acggacctgc aagacgccat agtatgaggc cgaatggctc actgtcaacg aacgttgtgt
     4561 gttgatcgtg attgcgggca ctcgggtgag accagtccgc ccgccggcag tggacgctct
     4621 catgggtccc cctcatcgaa gcggttctcc tgtgaccacc cgccggcacc gtccagcgag
     4681 atgaactgct gaccgactgc atgggcgagc gtcgcgtcgt gtgtgaccat caccagcgcg
     4741 ccggtgaacc gcgacagcgc ccactccagc agcgccagcg cgtcgatggc caggttcccc
     4801 tccagctcgt cgatgacgag aaggtcgggc cgcgccgcga cgaggcgcgc gatgaagaca
     4861 cgcttctgct gtcccgcgga aagaagcccg accggccgga agacctggtc gtcctcgatg
     4921 cccagcgtcc cgaggagggt gcgtgcgtcc tcgctgccta tgccgcccgc ctcgcgcacg
     4981 aggtcgacgg gaagctgctc accatggtcc agaacgatct gctgtggcac gaagccgact
     5041 ctgaccgatg gtgacagggt cacgtctccg ccgtccggct gccgcatccc tgtcatgatc
     5101 tggagcagtg tggacttgcc cgccccgttt ggtcccagca ggcaccagtg ctcgccactg
     5161 cggacggtca gcgacaggtc gtggaacagg gtacggtccc cggccgaagc cgtgacgcgc
     5221 accaacgata ccaggatgcc gccacgggtg gacccctcga ggacgtagtc gcgtttcttc
     5281 tcgatgaacg gtttcgccgc ggttgcctcg gcaagcgacc gttccagctg ggttcgcatg
     5341 gccttggcac gcttctccat tttcgctgcc ttgtggctga agaaccctga gcttgacgca
     5401 taggccagtg cctcgatgcc tccagcctgc cccttgaccc tgccgatggc ccgcttcagc
     5461 tgccggacct tgcgcgtctg cactgcgtag acgtcgcggt catgttcctc ctgcgcgcgc
     5521 acggtgtcga tgtactgtgt gtagccgccg gagtacagcc gcccgtgccc acgcgtcagc
     5581 tcgaggactt gcgtacagac caggtccagg aagcgccggt catgtgagac gactacgtat
     5641 ggccggtcga ggtcctggag cactgcagcc agctctgctc gcttctggat gtcgagatgt
     5701 gaggtgggct cgtcgagaac caggagatcc gcccgcgcga tcagtgcttc tgcaacgcgg
     5761 agccacaggc gttcacccac agatagtgac ttaactgtat ggtcccagag tgcagcgggc
     5821 agtccggcgt ctgccagtgt gtgctggacc tccgccagga ccgcgtagcc gccctgtgcg
     5881 gcaaactcgg cggcagcttc tcctgcgctg tcagcagcag ggcctgattc catcgcgctc
     5941 cacatggccc cgagcgccgg gtcgcgaaca ccgatggcgc ttgccaggac tgatctgatg
     6001 tcaccggcgt cgacctcgat gagcccgatc cggggagtcg gcctggacct gccgatggtg
     6061 cctgtggtcg gctgcagtct acccgccagc agacccagca gggtcgactt gccgctgccg
     6121 ttgggaccga agatgccgag gcgggaactg tccgagatcg agaaggtgac cccgtcaaac
     6181 aggagcgtgg cttgttcagg atatgagaag gagagatctg tacaggtgat ctgcatatgt
     6241 tcctccgtgt tcgtcgtcat gatgccgggg gcatgtgacg aaattgtcgc tacggtatga
     6301 cggaactatg caatacgcac tcgaagtacc tccatgggcg acaggcaaag tcaggtcgca
     6361 cgaccatggt acagcgaact atcgtcttgg caaggaccgg ttctctcagg ggttccggaa
     6421 gaggtacagg ccgaatccaa gtgttgctct gccccacgcc agataggcat gactccagga
     6481 gcggatccgt tccagcatcg cgtcgtggtc tggatcgtcg gggttgtcat gggcgaagtt
     6541 ctcgacgttc atatggtagc gccattcgta ctcgtcccac tcgtcatcat tggcgacact
     6601 agcccagaga ggtatgagcc ccaacttctc ggcaattccg atattgccct catgggtgcc
     6661 gtagacggac tcctcacact ccatgaagct cagatactcg ggtgaggggt gctgcttcca
     6721 gtagcagtct gcaaccagga gccagcctcc cggccgtacg caggcttcca gtcgttcgag
     6781 cgcgtccgcg taggtgccga aggcatgtgt cgatccaata c
//
LOCUS       DATMZD010000204         3870 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_106315,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000204 DATMZD010000000
VERSION     DATMZD010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3870
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106315"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..166
                     /locus_tag="VN478_04245"
     CDS             <1..166
                     /locus_tag="VN478_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011058040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HWQ21919.1"
                     /translation="IPVLEADRRAGDPAVLVAGNLRARRELGWTPQVADLETIIRTAW
                     DWEQHRQIGE"
     gene            286..1902
                     /locus_tag="VN478_04250"
     CDS             286..1902
                     /locus_tag="VN478_04250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HWQ21920.1"
                     /translation="MRGQYLSLAMNLVIGLSFFLYGMKLLGDGLQKAAGDSLRRILQA
                     LTNKPIRGVLVGMLVTGIIQSSGATTVMVVGFANAGLMTLRQAMGVIFGANIGTTITA
                     QIIVLKLDKLVWFFMLVGVMMEFFVKRKTSKAVGEAILGFGILFFGLYFMADTLAPLK
                     DNQGFINFLVRFGQVPILGVGAGAVFTALIQSSSVSTSLIVALAMKGMITLPSAIALI
                     LGANIGTTLTAGLASLGANITSRRAALTHFLFNFLGTVIMFPFLKPFARLVQLTATSL
                     PHQVANAHTIFNVIMTIIALLLINPFEKLVMRLLPSKEKEVETRVVQFIDDRVLVTPS
                     VALSQATQELYRMGHIAYEMVDNCRIALFENKMNLLENVLGNEELVNSMQKEITGYLT
                     KITEHDLSEAQGTRVMALMHAVNDIERVGDHATNLVELIQIKDDKRLKFMNGTQDDLK
                     AEFAHVLSTLDEAMEALRDYDVERAHRVKDMEDRCDVMTRECMSRNISRLSAHELDPQ
                     VGVVVVDLFTNLERISDHSDNIADVVLGVY"
     gene            complement(1984..2724)
                     /locus_tag="VN478_04255"
     CDS             complement(1984..2724)
                     /locus_tag="VN478_04255"
                     /inference="COORDINATES: protein motif:HMM:NF014228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HWQ21921.1"
                     /translation="MMNAQKLAGLIEKSHHIVALTGAGISTSAGIPDFRGPNGIYVTH
                     QYDPEKTFDIDWFDTDPRTFFSFARDFLSTVDRIHPTQTHTFLAELEQQGRLWSVITQ
                     NIDGLHQKAGSRNVIEVHGSFQRGHCRKCGHGYSCDELKGKILAEEVPHCDRCGGVIK
                     PDIVFYGEAVRDMEEAESEARHADLFLAIGTSLSVYPAATLPTFSGGPVISINRGALG
                     LSVHGIESIERDIDEVFTEVRSTLSGSS"
     gene            complement(2806..3672)
                     /locus_tag="VN478_04260"
     CDS             complement(2806..3672)
                     /locus_tag="VN478_04260"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HWQ21922.1"
                     /translation="MTADIRIGTCGYSFPDWKGVVYPPNVRPSLYLVYYARTLGFNAV
                     ELDSSFYHMPTISLIEALLQKTPPGFLFTVKAHRSMTHDVWNAMKPANPGRPAFPELA
                     SGLKSAALLAPAFTEFWEALIPMVDAGRLGTVVLQYPPWFRDTPENRRFLQMTRDLLP
                     RLSLSIEFRDQSWHTEGSLMFLRQEHLGYVAVDEPQLRNLLPMVPAVTSDVAYLRLHG
                     RNPRWFGASREERYDYLYSREELEQMLPSVRTMAASAPLMFVMTNNCHRGQAVQNARD
                     LQTMLFPEQKRS"
     gene            complement(3669..>3870)
                     /locus_tag="VN478_04265"
     CDS             complement(3669..>3870)
                     /locus_tag="VN478_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21923.1"
                     /translation="RLKTTQEQLAMDEGDLSSFAVRVRVVKNKLAPPEREVLLYYSLA
                     DGTLAEEPPAMVAEEPGEEPWP"
BASE COUNT          780 a         1109 c         1157 g          824 t
ORIGIN      
        1 catacccgtt cttgaggcgg atcgtcgggc cggagacccc gcggtgctcg tagcggggaa
       61 cctgcgtgcg cgcagggaac tcggctggac acctcaggtg gctgacctgg agaccatcat
      121 caggactgcg tgggactggg agcagcatcg gcagatcgga gagtgatgca tgacgacgat
      181 atgccgtctt ttcccatttg ccctttgacc ggtcgacgta atcccgtaga atacatcggc
      241 ggcaacatac cagaccgacc cgaaaactga tcctcggagg cacgaatgcg tggccagtac
      301 ctctcgttag ccatgaacct cgtcattggg ctgtcgttct tcctgtacgg catgaagctg
      361 ttgggcgacg gacttcagaa agcagccggg gactccctga gacgtatcct gcaggccctt
      421 acgaacaagc cgatccgcgg agtactggtc ggtatgctgg tcaccggcat catccagagc
      481 agcggagcca cgacagtcat ggtcgtcgga tttgccaacg ccgggcttat gacgctgagg
      541 caggccatgg gtgtcatttt cggcgccaac attggcacaa ccatcacagc ccagatcata
      601 gtgctcaagc tggacaagct cgtgtggttc ttcatgctgg tgggcgtcat gatggagttc
      661 ttcgtcaagc gcaagaccag caaggctgtc ggcgaagcca ttctgggctt cggtatcctg
      721 ttcttcggtc tctacttcat ggccgacaca cttgcgccgc taaaggacaa ccagggcttc
      781 atcaacttcc ttgtgcggtt tgggcaggtt cccatcctgg gggtcggcgc gggagctgtc
      841 tttactgcac tcatccagag ctcgtcagtg agtacgagct tgatcgtggc cttggcgatg
      901 aagggcatga tcaccctgcc gtcggctatt gctctgatcc tcggggcaaa catcgggaca
      961 accttgaccg cagggcttgc gagtctcggt gccaacatca ctagcagacg agcggcgctt
     1021 acccacttcc tgttcaattt cctgggtaca gtaatcatgt tcccgttcct gaaaccgttt
     1081 gcccgtctgg tgcagctcac cgcaacgagc cttcctcacc aagtagccaa cgcacacacg
     1141 attttcaacg tcatcatgac gatcattgcc ctgcttctca tcaacccgtt cgagaagttg
     1201 gtcatgcggc tcctgccgtc caaggagaag gaagtcgaga cgcgggtcgt ccagttcatc
     1261 gacgaccgcg tccttgtgac gccgtccgta gccctctctc aggcgacgca ggaactctac
     1321 cgcatggggc acatcgcgta tgagatggtg gacaactgtc ggatcgcgct gttcgagaac
     1381 aagatgaacc ttctcgagaa tgtcctcggc aatgaagagc tcgtgaactc catgcagaag
     1441 gagatcacgg gctaccttac caagatcacc gagcacgacc tgtctgaggc gcaaggcacg
     1501 cgtgtcatgg ccctgatgca tgcggtcaac gacattgaac gcgtgggaga ccatgccacg
     1561 aaccttgtcg aactcatcca gatcaaggac gacaagagac tcaagttcat gaacggcacg
     1621 caggatgacc tgaaggccga gtttgcccat gttctgagca ccctcgacga ggctatggag
     1681 gcacttcggg actacgatgt cgagcgcgct caccgggtga aggacatgga agaccgctgt
     1741 gatgtgatga ccagggagtg catgtcgagg aacatctccc ggctgagtgc gcatgaactg
     1801 gacccgcagg tcggcgtcgt ggtcgtggac ctgttcacga acctcgagcg catctcagac
     1861 cactccgaca atatcgccga cgtcgtcctg ggcgtctact agtcaatctt cgcctgggaa
     1921 catggtgagc ccccgcctca tgggcggggg ctccttgctt cgagacggct cttgccgctt
     1981 gcgtcaggag ctaccagaga gtgtgctcct gacctcagtg aacacctcgt cgatgtcgcg
     2041 ttcgatcgac tcgatcccat gaacagacag gcctaaggct ccgcggttga tggaaatgac
     2101 ggggccgccg ctgaaggtcg gcagcgttgc agcaggatag acactcaggg acgttcctat
     2161 ggccaggaac aggtccgcgt ggcgagcctc tgattctgct tcttccatgt cccggaccgc
     2221 ttccccgtag aagacgatgt ccggcttgat cacgccgcca cagcgatcac aatgagggac
     2281 ttcctcggca aggatcttgc ctttgagttc atcacacgag tagccgtgtc cgcacttcct
     2341 gcagtgaccc cgctggaagc tgccgtgtac ctcgatgacg ttcctgctgc ctgctttctg
     2401 gtgaagaccg tcgatgttct gggtgatcac agaccagagc cggccttgct gttcgagttc
     2461 cgcaaggaag gtatgagttt gtgttggatg aatacggtcg acggtgctca ggaagtctcg
     2521 agcgaaggag aagaaggtgc gtgggtctgt gtcaaaccag tcaatgtcga aggtcttctc
     2581 gggatcatac tgatgggtca cgtagatccc gttcggtccc cggaagtccg ggatccctgc
     2641 cgaggtcgag attccagcac ccgtcagtgc cacgatgtga tgtgacttct cgataagtcc
     2701 tgccagtttc tgcgcgttca tcataggttg ctgcctcgcc atttcaatgt accggggtgg
     2761 gattcggcgc cagacctcgg ctatacggac agtatagtgc ggcgatcatg agcgcttctg
     2821 ttccgggaac aacatcgtct gcaggtcccg tgcattctgc acggcctgac cccggtgaca
     2881 gttgttggtc atgacgaaca tcagcggcgc gcttgcagcc atagtgcgga ccgatggaag
     2941 catctgctcc agttcctctc tcgagtagag atagtcatac cgttcttctc ggctcgctcc
     3001 gaaccagcgg gggttgcgtc cgtgcagccg gagataggcg acgtcactgg tgacggccgg
     3061 caccatgggc aggagattac gcaattgtgg ctcgtcgacg gcgacgtagc ccaggtgttc
     3121 ctgcctgagg aacatcagag agccctcggt gtgccagctt tgatcgcgga actcgatgga
     3181 cagagaaagg cgtggcagca ggtcgcgggt catctggagg aacctgcggt tctccggagt
     3241 gtcccggaac cacggtggat actggaggac cacagtcccg aggcggcctg cgtcaaccat
     3301 gggtatcaat gcctcccaga actctgtgaa tgcaggcgcc aggagtgctg cgctcttgag
     3361 tcccgaggcc agttccggga atgcagggcg tccgggattg gcgggcttca tggcattcca
     3421 tacgtcatgc gtcatgctcc gatgggcctt cacggtgaac agaaagccgg gtggcgtctt
     3481 ctggaggaga gcctcgatca gggaaatggt gggcatgtga tagaagctgc tgtccagttc
     3541 caccgcgttg aaaccaagcg tgcgagcgta gtagacgagg tacagtgagg ggcgtacgtt
     3601 gggcggatag acgacgccct tccaatctgg gaacgaatat ccgcacgtgc caatgcgtat
     3661 gtctgcagtc atggccacgg ttcctccccg ggttcctctg ccaccattgc cggcggttcc
     3721 tctgccagtg taccgtctgc cagcgaataa tacaggagta cctcgcgctc tggtggcgca
     3781 agtttgttct tgaccacgcg tacccgtacc gcaaaagaag acaggtctcc ttcgtccatg
     3841 gccagctgct cctgcgtcgt cttcaagcgc
//
LOCUS       DATMZD010000205         2782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_86514,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000205 DATMZD010000000
VERSION     DATMZD010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86514"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="VN478_04270"
     CDS             complement(<1..707)
                     /locus_tag="VN478_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21924.1"
                     /translation="MSITRFVIKKEDIDRTAWQQVLVEADQKLCSVYGKLFYQRAQEA
                     GKAHDSRTEAVYTFLGAVASPELKLETPSRDPFVPAADLQYSRTPLPEDFSADELHVI
                     KQLIETVQDAELRSRLADILWVRQGDASVASLAVDSYLSSALHLKDVVQWADGYDRLK
                     RSFDIAVFLGKETKAYRKTIDYLQKQLSEFGADEPTPWWAKLINLLLDAGEGNASRFA
                     SICGTSAQRCETAGQWET"
     gene            complement(743..1114)
                     /locus_tag="VN478_04275"
     CDS             complement(743..1114)
                     /locus_tag="VN478_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21925.1"
                     /translation="MTLAIEPAEPVTVEVALGCGTSRTVAVHWEEGTLYARAGSEPQV
                     AFQPSLEAWKRFWRAADKANIWNWDRVQHADQPVLWRIAVDSGAKRVTASGQSTATDE
                     FKTFVRALKKLLPGVSFPFST"
     gene            complement(1636..>2782)
                     /locus_tag="VN478_04280"
     CDS             complement(1636..>2782)
                     /locus_tag="VN478_04280"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWQ21926.1"
                     /translation="FNNLLTGILGNITLVRSSLAPTDPLKENLDAAETAARQAANLTK
                     GLLTFGRSAMVLPTPMNITTALDTTLALLRQSLPATVEIVRDDEKEAWNILADQSQIT
                     QILLNLTVNARDAMKDQGTLTVCTRNETIGEDYIQTHPFARTGEFVHLSVADTGSGIP
                     PAIMEHLFEPFHTTKPVGFGTGLGLSIVYGAVKQAGGWITAVSRQGAERTAPPVGQTC
                     RTPSDMRTSLGSGTTFDIYLPRCLQEPMRPSTPASIPQNAGSSTVLVVEDEPVVCVVA
                     QALLTRSGYHVMTAANGAQALEMIASHPGAIDLIMLDMTMPGMTTSEVVQAIRALNAT
                     VPILLNSGYTSSDAVKQLLADGSVQDFLSKPYELQDLVEKVHNLLHR"
BASE COUNT          496 a          807 c          885 g          594 t
ORIGIN      
        1 gtttcccatt gtccggcggt ctcgcagcgc tgcgcgctgg tcccgcagat actggcgaac
       61 cgggatgcgt tgccttcgcc tgcatccagc agcaggttga tgagcttggc ccaccagggc
      121 gtcggctcgt cagcgccgaa ctcgctgagc tgcttctgaa gatagtcgat ggtcttgcgg
      181 tatgccttgg tctccttgcc caggaagacg gcgatgtcga acgaacgctt gaggcggtcg
      241 tatccgtcag cccactggac gacatccttg agatgcaggg cactgctcag gtagctgtcg
      301 acggcgaggc tggcgacgct ggcatctccc tggcgtaccc agaggatgtc ggcgagccgg
      361 gaccgcagtt cagcgtcctg gacagtctcg atgagctgct tgatgacatg cagctcatcg
      421 gcggagaagt cttcgggtag cggcgtgcgt gagtactgaa gatcggcggc ggggacaaac
      481 gggtcgcgcg atggggtctc cagcttgagc tcgggcgaag cgacggcgcc gaggaacgta
      541 tagacagcct ctgtgcgcga gtcgtgcgcc ttgcctgctt cctgcgcgcg ctgatagaac
      601 aacttgccgt acaccgaaca gagtttctga tcggcttcga ccaggacctg ttgccaggcc
      661 gttcggtcga tgtcttcctt cttgatcacg aatctggtga ttgacacggg ttcctccgag
      721 tgaatgagtg ttgtgggagt tgtcacgtgg aaaaggggaa gctgacgcca ggaagcagtt
      781 tcttgagggc gcgcacaaac gtcttgaatt cgtccgtggc tgtcgactga ccagaggctg
      841 tgacgcgctt tgcgccgctg tccactgcaa ttcgccagag cacaggctgg tcggcgtgct
      901 gcacgcgatc ccagttccag atgttcgcct tgtcggcggc gcgccagaag cgcttccatg
      961 cttcaaggga gggctgaaac gcaacctgag gctccgagcc tgcacgcgcg tacagcgtcc
     1021 cttcttccca gtggaccgca acggtccgcg acgtgccaca gccaagcgcc acttccacag
     1081 tgacaggctc ggccggctca atagcaagag tcatatagta caatcctcct tgcggccagt
     1141 tcgctgtccc acggcaccgg acggccacac gcacgacttg ggggtgcgat cacccgcgca
     1201 ctcggcagct caggagtccc cttaaggacc cgcagacgcc cgtgcaccac ggatattata
     1261 gcggattagt agcgcgacaa caggtgtacg tttcgatggt ccggagcggt cacgccgaaa
     1321 gaaggaaatc gcccggcagg gcagccgggc gatgtggagg gaggagaaga ggggaaggtg
     1381 gtcaaaacct tccttgtgtg tcctgttggc tcatccgaga tccagctctt gccggccggg
     1441 cccccggtgt tctgttgcca cctgtgctac acgtttcttg agacatccgt acatggtcgc
     1501 cttcatgttc gcgcttcgcc ttcgttggaa gcggcctgca agcttcctcc cacgtctgtc
     1561 tccatgcgca cgaaccaacg actggagatc ccccgagtac gatcggaggg gtctcgacgg
     1621 gaaacgcgat tctcgctatc tgtgcagcag gttgtggacc ttctcgacca ggtcctgaag
     1681 ctcgtatggc ttgctcagga agtcctgaac actgccatcc gcaagcagct gcttgacggc
     1741 gtcactggag gtgtaccctg agttcagcag gatgggaaca gtggcattca gggcccgaat
     1801 ggcttgtacg acttcgctgg tcgtcatgcc tggcatggtc atgtccagca tgatgagatc
     1861 aattgctcct ggatgcgacg cgatcatctc gagggcctgc gcgccattcg ccgcggtcat
     1921 gacatggtat ccgcttcgcg tgagcagagc ctgggcaact acgcacacga ccggttcatc
     1981 ctcgaccacc aggacggtac tgctgccggc gttctgtggg atagacgccg gagtggaggg
     2041 ccgcatgggt tcttgcaggc agcggggcag atagatgtcg aacgttgtcc ccgagcccaa
     2101 ggatgttctc atatccgagg gtgttctgca cgtttgtccg acgggagggg cggttcgttc
     2161 ggcgccctgc ctggagaccg ctgtgatcca gcctccagcc tgcttcacgg caccgtagac
     2221 gatggagagt cccagtccgg tgccgaaacc aaccggcttt gtcgtatgaa agggctcgaa
     2281 gaggtgctcc atgattgccg gggggattcc tgacccggta tccgcgacgc tcagatggac
     2341 gaactcgccc gtgcgtgcga atggatgggt ctggatgtag tcctcaccta tggtctcatt
     2401 cctggtgcat accgtcagtg ttccttggtc cttcatggcg tcacgcgcgt tcacggtgag
     2461 gttgagcagg atctgggtga tctgtgactg gtctgccagt atgttccatg cttccttctc
     2521 gtcgtcacgc acgatctcca cggttgcggg cagcgattgc ctcagcagcg ccagggttgt
     2581 gtcgagtgcc gtcgtgatat tcatgggtgt cggcaggacc atcgcgctgc gaccaaaggt
     2641 caacaaccct ttcgtgaggt tggctgcctg acgcgctgcc gtctctgccg catccagatt
     2701 ctccttcagt ggatctgtcg gggccaggct gctgcgaacg agggtgatgt tgcccaggat
     2761 gccggtcagc aggttgttga ag
//
LOCUS       DATMZD010000206         4156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_75600,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000206 DATMZD010000000
VERSION     DATMZD010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75600"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1496)
                     /locus_tag="VN478_04285"
     CDS             complement(<1..1496)
                     /locus_tag="VN478_04285"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HWQ21927.1"
                     /translation="MKAVILAGGFGTRLRPLTINLPKPMVPFANRPMMQHIVRLLKKH
                     GFDELVVILYHQPDAIRSYFGDGSDFGVKIEYVISQEDLGTAGAVGLARASLQETFLV
                     IAADIVTDIDLTKAVQYHREHKAAATITLSRVEDPLEYGIVITDTEGHVVRFLEKPSW
                     GEVFSDTVNTGIYVLEPSVWDDIPEDKEVDFSKNLFPALLAHNQPLFGYIAPGYWRDV
                     GNIREYKRAHGDLLKGRVDLGPQGERVGGIGSDVWVGEGSQISTFKGFDGSVLIGKGA
                     TIGEARVRSSFVGDRAVVGNGANIDNSILWDGVVVESGAHIFNSIVGRNVHVGKDVLL
                     EGDNTVADDCIIGQGSILKSQVQIWPGKTVEAGSTVTSNVVWGKAWSRALFSSYGIVG
                     VNNVEVTPEMCAKLGCAYGTFLGKGSVVFTARDDHQSSRMLKRSVIGGLMSAGVRVMD
                     LQAAPVPLARFAVKSFAGSGGVHVIRSPFDRRLTTIRFYDRSGFDLSTT"
     gene            complement(1493..2911)
                     /gene="glgA"
                     /locus_tag="VN478_04290"
     CDS             complement(1493..2911)
                     /gene="glgA"
                     /locus_tag="VN478_04290"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF001899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HWQ21928.1"
                     /translation="MRVFFVASEALPYAKTGGLGDVTGSLPAALRALGADAFLILPFY
                     RTIMAKNLPLKQVMTRTLTMGGRTLTYTVLRHEQTYFIAQDEFFDRDSLYNTREGDYP
                     DNWLRFAFFARAALETIVALGGADVIHVHDWQAALLPVYLRVLYPERHEKTLLTIHNI
                     AYQGLFSAEILPDIGLPSSVFTVDGLEFYGNVNYLKGGIVWADEVNTVSPTYAREIQT
                     DEYSFGLHGILATRRDHLCGILNGIDCSYWDPSADGALPYMYSLASLVGKEQDKDSLL
                     EEAGLVARPDCPLFVMVSRLVAQKGVDLMLATFDQMMSLRLSLMVLGTGDAEIETALV
                     AKAAAYPGRFVLSRKFDEGLAHRMYAGSDFFLMPSRFEPCGLSQMIALRYGSVPVVRR
                     TGGLKDTVQDIHPKTGAGNGISFDDALPGEFLEAVRRAVTLYADGDAFQRVRAIGMSC
                     DFSWQASARDYLTLYHSMEVSQ"
     gene            complement(2908..3912)
                     /gene="galT"
                     /locus_tag="VN478_04295"
     CDS             complement(2908..3912)
                     /gene="galT"
                     /locus_tag="VN478_04295"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HWQ21929.1"
                     /translation="MPELRKDPVIGRWVIIATERALRPSNFRTDKEEHLTDLSQCPFE
                     PGNEASTPPEILSYRRSDSRPNEPGWWVRVIPNKFPALRIEGTMDKHGDGMYDMMNGI
                     GAHEIVIETPNHDEEMAEMPEAQVKEVLWAWRDRIVDLARDRRFKYVMVFKNKGALAG
                     ASLQHPHSQIIALPIVPLRVQEELTGAAQYYEYKDRCVFCDIIQQELSDRKRIVEDSE
                     RFIAVVPFAQRFPFETWILPKQHESDYSRATRTDFLELAGLLKRTLQRLSRALDDPPF
                     NLVLHMAPLNTEGLPYYHWHLEIMPRVTRTAGFEWGTGFYINPTKPEETAQFLREVQL
                     "
     gene            complement(3912..>4156)
                     /locus_tag="VN478_04300"
     CDS             complement(3912..>4156)
                     /locus_tag="VN478_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013468430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein
                     domain-containing protein"
                     /protein_id="HWQ21930.1"
                     /translation="HGPASDMLLREMLLEESSDWPFLITTGQARTYGTTRFLEHHRKV
                     RALFAAIEGTGQLPADQTGSDDAVFEHIHLDDVRRI"
BASE COUNT          827 a         1219 c         1234 g          876 t
ORIGIN      
        1 gtcgtggaga ggtcgaaccc tgatcggtca tagaatcgta ttgtggtcag acgacgatcg
       61 aacggtgagc ggatcacgtg aacgccgccg ctgcccgcaa atgacttgac ggcgaaccgg
      121 gccaacggca ccggagccgc ctgaaggtcc atgacacgta ccccggcact catgaggcca
      181 ccgatgacag agcgcttgag catgcgagac gactggtggt cgtcgcgtgc agtgaagacg
      241 actgaaccct tgccgaggaa cgtgccgtag gcacaaccca acttggcgca catctcggga
      301 gtgacttcaa cgttgttgac gccgacgatg ccataggagc tgaagagggc ccttgaccag
      361 gccttgcccc atacgacgtt gcttgtaacg gtcgaacctg cctcgacggt cttgcccggc
      421 catatctgga cctgggactt gaggatggat ccctgtccga tgatgcagtc gtcggctacg
      481 gtgttatccc cctcgagcag gacatccttg ccaacgtgga cattgcggcc cacaatggaa
      541 ttgaagatgt gggctcctga ttccacgacg acgccgtccc acaggatcga gttgtcgatg
      601 tttgctccat ttccgacgac agcgcggtca cctacgaagg agctgcgcac tcgcgcctcg
      661 cctatggtcg cacccttgcc gatgagaacc gaaccatcga atcccttgaa ggttgagatc
      721 tgacttccct cgccaaccca gacatcacta ccgatgcctc cgacacgctc gccctgggga
      781 cctaggtcga ctcttccttt caggagatct ccatgggcgc gtttgtactc gcggatgttg
      841 cctacgtcgc gccagtagcc cggggctatg tacccgaaca gcggctgatt gtgtgcaagc
      901 agtgcgggga acagattctt ggagaagtcg acctccttgt cctccgggat gtcgtcccac
      961 acggacggct ccaatacgta gattcctgtg ttcactgtat ccgagaagac ttcgccccac
     1021 gatggcttct cgaggaaacg cacgacgtgc ccttcggtgt cggtgatgac gatgccatac
     1081 tcgagggggt cctccactcg agacagagtg attgtagcgg ctgccttgtg ttcgcggtgg
     1141 tactgaactg ccttcgtgag gtcaatgtcg gtgacgatgt ctgccgcaat gaccaggaac
     1201 gtttcctgca ggctggcccg tgcgagccca acagctccgg cggtgccaag gtcctcctgg
     1261 ctgatcacat actcgatctt cactccgaag tcgctgccgt cgccaaagta gctgcgtatc
     1321 gcgtcgggct ggtgatacag gatgacgaca agctcgtcga acccgtgttt cttgagcagc
     1381 cttacgatat gctgcatcat tggacggttg gcgaaaggga ccatgggctt tggcaggttg
     1441 atggtcagcg gacgcaaccg ggtgccaaag cctcctgcca ggattactgc cttcactgag
     1501 acacctccat ggaatggtat agtgtgaggt aatcgcgtgc gctggcttgc catgagaagt
     1561 cacaggacat gccaatggcc cggacacgct ggaaggcgtc accatctgcg tacagtgtga
     1621 ccgcacgtct cacggcctca aggaactcgc cgggcagcgc gtcgtcgaag ctgatgccgt
     1681 tcccggcccc tgtcttcgga tggatgtcct ggaccgtgtc cttcagcccc cccgtcctgc
     1741 gcaccacagg gacgcttcca tagcgcagcg cgatcatctg agagaggccg cacggctcga
     1801 atcgcgatgg catgagaaag aagtccgatc cggcgtacat gcggtgtgca aggccttcgt
     1861 cgaacttgcg gctcaggacg aagcgcccgg gatatgcagc ggctttggcc acaagggccg
     1921 tttcgatctc ggcatcgccg gtgcccagaa ccatgaggct caagcgaagt gacatcatct
     1981 gatcgaatgt ggcgagcatc aggtcgactc ccttctgggc aaccaaccgg gataccatca
     2041 caaacagggg acagtcaggc cgtgccacca acccagcttc ctctagcagg ctatccttgt
     2101 cctgttcctt gccgacaagc gacgcgagac tgtacatgta ggggagtgct ccatccgcag
     2161 aagggtccca ataggagcag tcgatgccgt tcagaatgcc gcacaggtgg tcacgccgcg
     2221 ttgccagaat gccgtgcagc ccaaaactgt actcatccgt ctggatctcg cgggcgtatg
     2281 tcggggagac agtgttgact tcgtcggccc agacgatgcc ccccttgagg tagttgacgt
     2341 tgccgtagaa ctccaggccg tccactgtga acaccgagct cggcaagccg atgtcaggca
     2401 ggatctcggc agagaacagt ccctgatagg cgatgttgtg gatcgtgagc aatgtcttct
     2461 cgtggcgctc tggatacagg acgcgcagat acacgggcag caacgcggct tgccagtcgt
     2521 gcacgtggat gacgtcagcc ccgccgagag cgacaatggt ctcgagtgcc gcacgtgcaa
     2581 agaacgcaaa ccgcaaccag ttgtccggat agtcgccttc acgcgtgtta tacagactgt
     2641 cgcggtcaaa gaactcgtcc tgagcgatga agtacgtctg ttcgtggcgc agtaccgtgt
     2701 acgtcagagt ccttccaccc atcgtcagcg tgcgggtcat gacctgcttc agcggcaggt
     2761 tctttgccat gatcgtgcgg tagaagggca gaatcaggaa ggcgtctgcg ccgagcgcgc
     2821 gcaaggccgc gggaaggctc cccgtcacgt ctccaaggcc gccggtcttg gcatagggca
     2881 gtgcctccga ggctacgaag aagactctca cagctgtacc tcgcgcaaga actgcgctgt
     2941 ctcttctggt ttcgtcggat tgatgtagaa acctgttccc cattcgaatc cagcagtgcg
     3001 tgtgacacgt ggcatgatct ccaggtgcca gtggtagtat ggaagtcctt cagtgttgag
     3061 aggagccatg tgcagcacga ggttgaaggg tgggtcgtca agtgctcggc tcagcctttg
     3121 gagagtacgt ttcaacagac ccgcgagctc caggaagtca gtccgtgttg ctcgtgagta
     3181 gtcgctttca tgctgcttgg gcaagatcca cgtttcgaat gggaagcgct gcgcgaacgg
     3241 cacgacagct atgaaccgtt cactgtcctc gacgatgcgt tttcggtcgg acagctcctg
     3301 ctggatgatg tcacagaaga cacaccggtc tttgtactcg tagtattgtg ctgcccctgt
     3361 caactcctcc tgtacgcgca agggcacgat ggggagagcg atgatctggg aatgggggtg
     3421 ctgcagggaa gcgcccgcca gcgcaccctt gttcttgaat accatgacat acttgaaacg
     3481 cctatcgcgg gcgaggtcca caatgcggtc tctccatgcc cagagcactt ccttgacctg
     3541 cgcctcgggc atctcggcca tttcctcatc atggttggga gtctcaatga cgatctcgtg
     3601 ggctccaatg ccgttcatca tgtcatacat gccgtcacca tgcttgtcca tggttccttc
     3661 gattctgaga gccgggaact tgttggggat gacacgcacc caccaacctg gttcattggg
     3721 ccttgagtcc gatctacgat acgagaggat ctccggtggt gtgctggcct cgttcccagg
     3781 ttcgaacggg cactgggaca gatcggtaag gtgctcttcc ttgtctgtcc tgaagttgct
     3841 ggggcgcaat gcacgctcgg tggcaatgat gacccaccgc ccgatgacag gatccttgcg
     3901 cagttccggc atcaaatcct ccttacgtcg tccagatgga tatgctcgaa cacggcgtca
     3961 tctgaccccg tctggtctgc agggagctgc ccggtgccct cgatcgccgc gaacaacgcc
     4021 cgtactttcc ggtggtgttc gaggaatctc gtcgtgccgt aggttcgggc ttgcccggtc
     4081 gttatcagga agggccaatc ggagctctcc tcgagcagca tctctcgcag cagcatgtcg
     4141 ctggccgggc catgaa
//
LOCUS       DATMZD010000207         2831 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_36831,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000207 DATMZD010000000
VERSION     DATMZD010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36831"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VN478_04305"
     CDS             complement(<1..184)
                     /locus_tag="VN478_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21931.1"
                     /translation="MKNNLLLFLRTLNARAYVRIFGQMREATWVIASVVGGFFTMTTY
                     IYLYRALGTGSEFSGLV"
     gene            complement(156..1160)
                     /locus_tag="VN478_04310"
     CDS             complement(156..1160)
                     /locus_tag="VN478_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014452763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWQ21932.1"
                     /translation="MHTSIQVENLTRVFNRRGSAPFHALRGIDFDVSYGEIFGLLGPN
                     GAGKTTTIKIMSTLLLPTSGRVTVAGFDVERDYARVRPFISLISGGETSGYGILRVEE
                     QLWMFSQFYGMETPVARTRIEHYLRMVGMWDDRRQQMNRLSTGMRQKVNLVRGLVTDP
                     KVLFLDEPTLGVDVEASVIIRGIVRDWVIEKPERSVILTTHYMAEADELCSRVAIINR
                     GLILANAAPSRLKQDLDSRAHYEITVDGISQEQIDGIATDMGEGNDFSLNSDKVTDSP
                     MLVAHLTSEGGISRLIALLTEQSISVEYMRKLEPTLEDVFLKMVGKRFEDEEQPAPVS
                     "
     gene            complement(1666..2166)
                     /locus_tag="VN478_04315"
     CDS             complement(1666..2166)
                     /locus_tag="VN478_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-thioredoxin reductase catalytic
                     domain-containing protein"
                     /protein_id="HWQ21933.1"
                     /translation="MSKIADDRVDEVWQRLVLEARDAGYSVNPDDAFARDLVRGLLEN
                     EERYGYRACPCRLASGVADEDLDLVCPCDYRDADLNDYGVCYCALYVSPGIAAGTQKA
                     VSIPDRRPVGGPAKHVESTERLDVRGLAFPVWRCKVCGYLCARPQPPLVCPVCKAGKE
                     RFERFM"
     gene            complement(2163..2450)
                     /locus_tag="VN478_04320"
     CDS             complement(2163..2450)
                     /locus_tag="VN478_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012901144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HWQ21934.1"
                     /translation="MLEPEHVEGAHTKHTVFIYALSTCGWCRKTKALLQELSVAYDFV
                     DVDQQEGSNKDLAKQEVLKWNPACSFPTIVVDGKDCLVWFQEDKLRELAAE"
     gene            complement(2478..>2831)
                     /locus_tag="VN478_04325"
     CDS             complement(2478..>2831)
                     /locus_tag="VN478_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012543447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HWQ21935.1"
                     /translation="RLGGGLRQVGILAAAGIVALETMVDRLAEDHANAKLLASELASI
                     GYGTDPSEAETNMAMVDVTASGKDAPTFVAAAREKGVLCNAVTPTTIRLVTHHDVTTD
                     QCRETVAVFGSLLHG"
BASE COUNT          587 a          878 c          771 g          595 t
ORIGIN      
        1 ccacgagtcc gctgaactcg gaacccgtgc caagcgcccg atacaggtag atgtacgtcg
       61 tcatcgtgaa gaagccaccc acgacgctcg caatcaccca ggtcgcctcg cgcatctgcc
      121 caaagatacg cacgtaggca cgtgcgttga gcgttctaag aaacaggagc aggttgttct
      181 tcatcttcga acctcttgcc gaccatcttg aggaagacgt cttccagtgt cggctccagc
      241 ttgcgcatgt attcgaccga gatagattgc tctgtcagca aggcgatcag tcgcgagatg
      301 ccgccttcgc tggtcaagtg cgcgaccagc atggggctgt cggttacctt gtcggagttc
      361 agggagaagt cgtttccttc gcccatgtcc gtcgcaatac catctatctg ctcctggctg
      421 atcccatcca ctgtgatctc atagtgcgct ctgctgtcca gatcttgctt gagtcgtgac
      481 ggagccgcgt tggcgaggat gaggccgcgg ttgattatag ccacccgcga acagagttca
      541 tcagcctccg ccatgtaatg tgttgtaagg atgaccgagc gttccggctt ctcgatcacc
      601 caatctcgga caatgccacg gatgatgacg ctcgcctcca cgtcaacccc cagggtgggt
      661 tcgtcgagga acagcacctt agggtcagtg acgaggccgc gcacaaggtt cactttctgc
      721 ctcatgcctg tggacagacg gttcatctgc tgacgcctgt catcccacat gccgaccatt
      781 cgcagatagt gctcgatcct ggtacgggca actggggtct ccatgccgta aaactggctg
      841 aacatccaca gctgctcctc gaccctgagt atgccgtatc ccgacgtctc gccccctgag
      901 atcagactga tgaaaggacg tacgcgggca tagtcccgtt ccacgtcaaa ccccgcgaca
      961 gtgacacggc ccgacgtcgg aagcagaagg gttgacatga tcttgatggt cgtggtcttg
     1021 cccgcaccgt tcggacccag cagaccgaag atctcgccat aggaaacgtc gaaatctatg
     1081 ccgcgcagcg cgtgaaaagg agctgatcct ctgcgattga agacacgagt gaggttctct
     1141 acttgaatag atgtatgcat acagtgctac ctccagaatg ggtgtgggga aaagcgaact
     1201 gtatgacagg agagactact gatggaccct gtgagagtca gtgactgagg aatcgattgt
     1261 cctgcgttta ctgtggcaaa ctcctgattg cagcttgtcc aatcaacggc acccctgtca
     1321 tcagttggtg cgaatcctgt tgtctaccat tacgtacctc cacatgcatg aatgtccctg
     1381 cgtgcgcctg gagcgcaact ttcagactat accagaacgt acttcctttg tcaatggttg
     1441 tacgtcgcgt catggggccc gttgccgacc gcaaatgaag gccgcgctca tgcgacggcc
     1501 gtcctcagtg tcagttgtga tatgagatga aaatcatcca ggaaccgcgc agctcatgag
     1561 aacatggcta gagacagccg gacatgcgac cctcgaagac gccaggagcc gccttgtccc
     1621 gagaagggga cgggggcggc tcctgacgac aatgcgcgtc tcaagctaca tgaaccgttc
     1681 gaaacgctcc ttgcctgcct tgcagacagg gcagaccaga ggcggctgtg gcctcgcaca
     1741 cagatacccg cagaccttgc agcgccagac tggaaacgcc agtccacgca catcgagacg
     1801 ctcggtgctc tccacgtgct tcgccgggcc accgactggt cgacggtccg ggatcgagac
     1861 ggccttctgc gtgccggctg caatccccgg cgaaacgtac agcgcgcagt agcagacgcc
     1921 gtagtcgttc aagtcagcgt cccggtagtc acacgggcat acgagatcca ggtcctcgtc
     1981 agcgactccc gacgcgagac ggcaggggca tgctctgtag ccgtaccgtt cctcattttc
     2041 cagcagcccc ctgaccaggt ccctggcaaa cgcatcgtca gggttgacag agtatccggc
     2101 atcccgagcc tcgaggacca gcctctgcca gacctcatcg acacgatcat ccgcgatctt
     2161 gctcattcag ccgcgagctc tcgaagcttg tcttcctgga accacaccag gcagtccttc
     2221 ccatccacaa cgatggtggg aaacgagcag gccgggttcc atttgaggac ctcctgcttg
     2281 gctagatcct tgttgcttcc ctcctgctgg tcgacatcga caaagtcata ggccacgctc
     2341 agttcctgca gcaacgcctt cgtcttgcga caccatccac aagtactcag tgcgtagatg
     2401 aagacagtgt gtttggtgtg ggccccttca acgtgttccg gttccagcat tgtttcctcc
     2461 ttgacgcagg tgcgttccta cccgtgcaat aatgatccaa agaccgcaac ggtctctcga
     2521 cactgatcgg tcgtcacgtc gtggtgcgta acaagccgaa tcgtggtcgg cgtgacagca
     2581 ttgcacaaga cacccttctc cctggctgcg gccacgaacg tcggcgcatc tttgccgctg
     2641 gcggtgacgt cgaccatggc catgttcgtt tcggcttcag acggatcagt cccgtatcca
     2701 atgctggcca gctcgctcgc gagcagcttc gcattggcat ggtcctccgc cagcctgtcc
     2761 accatcgtct caagcgccac gataccggct gccgccagaa tgccgacctg gcgcagtccg
     2821 cctcccaggc g
//
LOCUS       DATMZD010000208         5178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_75635,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000208 DATMZD010000000
VERSION     DATMZD010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5178
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75635"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /gene="rplJ"
                     /locus_tag="VN478_04330"
     CDS             complement(<1..310)
                     /gene="rplJ"
                     /locus_tag="VN478_04330"
                     /inference="COORDINATES: protein motif:HMM:NF012679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HWQ21936.1"
                     /translation="MLTKDQKKQLVDDLVKQLKESESIVFVGYEGLKAAFVANERKKL
                     TKQGIEYVVVKNTLLERAMKEVGFALPDSLFTQMTAVGLGKSDPSILAKALYKKPTNK
                     P"
     misc_feature    complement(328..460)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(468..1157)
                     /gene="rplA"
                     /locus_tag="VN478_04335"
     CDS             complement(468..1157)
                     /gene="rplA"
                     /locus_tag="VN478_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HWQ21937.1"
                     /translation="MSKKGKKYQEVAKLVDYAKIYTLDEAVELLPRLRTAKFDETIEV
                     CMKLNVDPKQADQNVRGVVTLPAGTGKTVRVAVFTKGDKSAEAQEAGADFVGGEELVE
                     KIKGGWLDFDVAIATPDMMATLGKVARVLGPRGLMPNPKAGTVTLEVGKAVREFKAGK
                     IEFRVDKIGTIHCPLGKSSFAPAAIKENFMSLFEAIQKARPSSVKGTYLGKTYLALTM
                     SPAVRLDTSKL"
     gene            complement(1219..1644)
                     /gene="rplK"
                     /locus_tag="VN478_04340"
     CDS             complement(1219..1644)
                     /gene="rplK"
                     /locus_tag="VN478_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012002521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HWQ21938.1"
                     /translation="MAKKIVGYARVQIEGGKATPAPPVGPALGQKGVNIMEFCKQFNE
                     RTKDKAGLVIPAVITVFEDKSFTFITKTPPASSLIKRAIKIESGSGEPNKKKVGRITK
                     AQLVEIAKIKMEDLNTADIEQAVKIIGGSAKSMGVTVVD"
     gene            complement(1675..2205)
                     /gene="nusG"
                     /locus_tag="VN478_04345"
     CDS             complement(1675..2205)
                     /gene="nusG"
                     /locus_tag="VN478_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HWQ21939.1"
                     /translation="MAERNWYLLHTYSGAEEKARNNLEQKVKTLALTDRVFSVVLPVD
                     NKVIIKNGERKVKQEKVYPGYLVVEMIMDDETWNVVTHTPGILGFVGNYGKPIPLSDE
                     EVERIIKNRVSEEEAVAKLHFEVGDTVQIIGGPFDGMQGKVESISPEKEKARIKLMMF
                     GREMPVEIDFGFIKRI"
     gene            complement(2212..2427)
                     /gene="secE"
                     /locus_tag="VN478_04350"
     CDS             complement(2212..2427)
                     /gene="secE"
                     /locus_tag="VN478_04350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HWQ21940.1"
                     /translation="MSNTKTIKPAGVRFKNWVRSIWTEIRYRVTWPRPRELAKSGVTI
                     IAFVAFWALYIGLWDYLFAAALKWLIS"
     gene            complement(2449..2524)
                     /locus_tag="VN478_04355"
     tRNA            complement(2449..2524)
                     /locus_tag="VN478_04355"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2489..2491),aa:Trp,seq:cca)
     gene            complement(2543..2692)
                     /gene="rpmG"
                     /locus_tag="VN478_04360"
     CDS             complement(2543..2692)
                     /gene="rpmG"
                     /locus_tag="VN478_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018704734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HWQ21941.1"
                     /translation="MRDIITLECTVCKNHNYTSIKNKKNDPDKVHVNKYCSHCRKHTE
                     HKETK"
     gene            complement(2702..3910)
                     /gene="tuf"
                     /locus_tag="VN478_04365"
     CDS             complement(2702..3910)
                     /gene="tuf"
                     /locus_tag="VN478_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012268992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HWQ21942.1"
                     /translation="MAKEKFERNKPHVNIGTIGHVDHGKTTLTAAITKCLATLGHTTV
                     KRYDEIDNAPEEKARGVTINAHHAEYETEKRHYAHIDCPGHADYIKNMITGAAQMDGA
                     ILVVASSEGPMPQTREHLLLAHQVNVPRIVVFLNKVDQVDDPELIDLVEMEVRDLLKK
                     EGFDGDATPIIRGSALKAMEYEGDCSRGKNEWTDKIWELMDAVDSYIPTPIRPTDKPF
                     LLAVEDVFTITGRGTVATGRIERGIVKMADNVEIVGLSEETRKTVVTGIEMFHKLLDD
                     GEAGDNVGLLLRGVEHDDVRRGQVICKPGSITPHTKFEAQVYILSQAEGGRHKPFFPG
                     YKPQFYFRTTDVTGSIALPEGQEMAMPGDNVVMTVTLMTHIAMEEGLRFAIREGGHTV
                     GAGVVTKILE"
     gene            complement(3952..4027)
                     /locus_tag="VN478_04370"
     tRNA            complement(3952..4027)
                     /locus_tag="VN478_04370"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3992..3994),aa:Thr,seq:ggt)
     gene            complement(4029..4105)
                     /locus_tag="VN478_04375"
     tRNA            complement(4029..4105)
                     /locus_tag="VN478_04375"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4069..4071),aa:Met,seq:cat)
     gene            complement(4286..4360)
                     /locus_tag="VN478_04380"
     tRNA            complement(4286..4360)
                     /locus_tag="VN478_04380"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4326..4328),aa:Gly,seq:tcc)
     gene            complement(4369..4453)
                     /locus_tag="VN478_04385"
     tRNA            complement(4369..4453)
                     /locus_tag="VN478_04385"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4417..4419),aa:Tyr,seq:gta)
     gene            complement(4458..4533)
                     /locus_tag="VN478_04390"
     tRNA            complement(4458..4533)
                     /locus_tag="VN478_04390"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4498..4500),aa:Thr,seq:tgt)
     gene            complement(4594..5073)
                     /locus_tag="VN478_04395"
     CDS             complement(4594..5073)
                     /locus_tag="VN478_04395"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWQ21943.1"
                     /translation="MGDNAAFEDLLGMYLPLLKFVARKYCTERQERDDCLAEAVMGFL
                     RAIRTYDVRRGSLDGYIATVASHRLIDMVRRTSGPHVELSDTLDGWGSSLGDPAEAGP
                     PDMAALTITLSDCERACFERRLRGETIDVIAAGLKMTKASVSNALARAKRKLAKALS"
BASE COUNT         1082 a         1533 c         1378 g         1185 t
ORIGIN      
        1 cgggcttgtt ggtcggcttc ttgtacaaag ccttcgctag aatggatggg tcgctcttgc
       61 ccagtccgac agcggtcatc tgcgtgaaca gggagtcagg cagtgcaaaa ccgacttcct
      121 tcatggctcg ctcaaggaga gtgttcttga cgacgacgta ctcgataccc tgcttcgtca
      181 gcttcttccg ctcattggcg acgaatgccg ccttcagccc ttcgtagccc acaaacacga
      241 tggactccga ctccttcagc tgcttgacaa gatcatcgac aagctgtttc ttttgatcct
      301 tggttagcat gtttcacctc ctctttgtat ggaaaggaaa aagctctctg ccaaagacag
      361 agagccgtat tgcacgcagt caagaagcgc aaacgcctcg gcgggcaaga ttgaggggat
      421 gaaccccgct cgctgtcttg ggctactagt tgtaacagaa gtgtactcta aagtttgctc
      481 gtgtccagcc gaaccgccgg actcatggtg agtgccaggt aggtcttgcc aaggtaggtg
      541 cccttgactg aagaagggcg ggccttctga atggcttcaa agagagacat gaagttctcc
      601 ttgatggcag caggagcgaa gctcgacttg ccaagagggc agtgtatcgt gccaatcttg
      661 tcgacccgga actcgatctt gccggccttg aattcacgga cagccttccc tacttcaagc
      721 gtgacggtac cggccttcgg gttgggcata aggccgcgtg ggccaagaac acgggccacc
      781 tttccaagag tcgccatcat gtccggggtc gcgatcgcaa cgtcgaaatc gagccagcca
      841 cccttaatct tctcgaccag ctcctccccg cctacgaagt ctgcaccggc ctcctgggcc
      901 tcggcggact tgtcaccctt ggtgaacacg gcaaccctga cggtcttgcc cgtaccagcc
      961 gggagcgtca cgacgccacg aacattctgg tctgcctgct tcggatcgac gttcaacttc
     1021 atacagactt cgatcgtctc gtcaaacttg gcagtcctca gtctcggcag caactcaacg
     1081 gcctcatcca gggtgtagat cttggcatag tcgacaagct tggcaacttc ctgatacttc
     1141 tttcccttct tgctcatgca ttacctccgt ggttacgtgg agtgactcct cccacgtgtg
     1201 ctatgaatct gctacctgct agtcgacgac cgtgactccc atgctcttgg cggagcctcc
     1261 aatgatcttg acagcctgct cgatgtcggc agtgttgagg tcctccatct ttatcttggc
     1321 gatctcgacc agctgagctt ttgtgatgcg accaaccttc ttcttgttcg gttcaccaga
     1381 tccagactcg atcttaatcg ctcgcttgat gagagaagac gccggcggcg tctttgtgat
     1441 gaacgtgaac gacttgtctt caaacactgt gatgacggcg ggaatgacca atccagcctt
     1501 gtccttggtc ctctcgttga actgcttgca gaattccatg atattgacgc ccttctgacc
     1561 aagagctgga ccgacaggcg gtgctggtgt cgcctttcct ccctcgatct gtacgcgcgc
     1621 gtaccctaca atcttcttcg ccatggtgga aactaccccc taaactcaga tctgctagat
     1681 cctcttgata aagccaaagt cgatctcgac cggcatctcg cgtccaaaca tcatgagctt
     1741 gatgcgagcc ttctccttct ccggcgagat gctttcgacc ttaccctgca ttccatcaaa
     1801 cgggccaccg ataatctgca cagtgtcgcc gacttcgaag tggagcttgg ccactgcctc
     1861 ttcctcggaa accctgttct tgataatccg ctcaacctct tcgtcagaaa gagggatggg
     1921 cttcccataa ttgccgacaa atcccagaat gccgggagtg tgcgtaacca cattccacgt
     1981 ctcgtcatcc ataatcatct caacgacgag gtatcccggg tagacctttt cctgcttcac
     2041 tttgcgctcc ccgttcttga tgatcacctt gttgtccacg gggagaacca cagaaaagac
     2101 gcggtctgtg agcgccagcg tcttgacctt ctgttccaag ttgttcctgg ccttttcctc
     2161 cgcacccgag tacgtatgca ggaggtacca gttgcgttca gccatccaag cctacgagat
     2221 gagccacttc agagcggctg caaacagata gtcccagagg ccgatataca gggcccagaa
     2281 agcgacgaaa gcgatgatcg ttactccgga cttcgccagt tcgcggggtc ggggccacgt
     2341 caccctatac cggatctcag tccagatgga ccgaacccag ttcttgaagc gcacgcctgc
     2401 aggcttgatt gtcttggtgt tactcactcg ttcctcctca gtggaaggtg gcaggcccgg
     2461 aggggctcga acccccaaca ctcggttttg gagaccgatg ctctaccagt tgaactacgg
     2521 gcctacctgt ggggacttgc cgctacttgg tctccttgtg ttccgtatgc tttctgcaat
     2581 gagaacagta tttgttcaca tgcactttgt ctgggtcgtt cttcttgttc ttgatgctgg
     2641 tgtagttgtg attcttgcac actgtgcact caagcgtaat gatatctctc atgacaggta
     2701 cctactccag aatcttggtg acgacgccag cgccgaccgt gtgaccaccc tcgcggatgg
     2761 cgaagcggag accttcctcc atggcgatgt gcgtcatgag ggtgacagtc attacgacat
     2821 tgtcgccagg catggccatt tcctgaccct cggggagggc gatggaaccg gtcacgtcgg
     2881 tggtgcggaa gtagaactgg ggcttgtagc cggggaagaa gggcttgtga cggccacctt
     2941 ctgcctggga caggatatag acctgtgcct cgaacttcgt gtgcggggtg atggaaccgg
     3001 gcttgcagat gacctgcccg cggcgcacgt catcatgctc aacaccacgg agcagcagac
     3061 ccacattgtc gcctgcctca ccgtcgtcga gcagcttgtg gaacatctcg ataccggtga
     3121 caacggtctt gcgggtctct tctgagagcc cgacgatttc gacgttgtcc gccatcttga
     3181 cgataccacg ctcaatacgt ccagtcgcaa cagtaccgcg gccggtgatc gtgaagacat
     3241 cctcgacggc aagcaggaac ggcttgtcgg tcggacggat gggagtcggg atgtaactat
     3301 cgacagcgtc catgagctcc cagatcttgt cggtccactc gttcttgcca cgagaacaat
     3361 ccccttcgta ctccatggcc ttgagggcgg aaccacggat gatgggggtt gcgtcaccat
     3421 caaagccttc cttcttcagg aggtcacgga cttccatctc gacgaggtcg atcagttcgg
     3481 gatcatcgac ctggtccacc ttgttgagga agaccacgat ccgtggaacg ttgacctggt
     3541 gagcgagcag gaggtgttcg cgcgtctgag gcatggggcc ttccgatgag gcaaccacaa
     3601 ggatcgcacc atccatctgg gcagcaccag tgatcatgtt cttgatgtaa tctgcgtggc
     3661 caggacaatc gatgtgcgca taatgacgct tctcggtctc atactcggcg tggtgggcat
     3721 tgatcgtgac gccgcgggcc ttctcttccg gagcgttgtc gatctcgtca taccgcttga
     3781 ccgtcgtatg gcccaaggta gcgaggcact tggtgatggc agcggtcaat gtcgtcttcc
     3841 catggtcgac gtggccaatg gttccgatat tgacgtgcgg cttgttacgc tcaaacttct
     3901 ctttcgccat tatagcctcc cgtgaaatgt gctctcgtgt atacaataaa atggagccca
     3961 cgaccagaat tgaactggtg accttatcct taccaaggat acgctctacc aactgagcta
     4021 catgggcttg gtcgcggggg accgatttga acggtcgacc tttgggttat gagcccaacg
     4081 agctaccagg ctgctccacc ccgcattcct cgctgtcgtt gtgcatatgc acacaaacag
     4141 cagtatgtat actactcgaa aagcacaaga agtcaatcac caccggagca ccgaacgctc
     4201 cgcaggaggt gccgcactac cgaagctacc cagagagggc atgcacgatc ccccagaacg
     4261 aggagtgcag ctccagccga aacgttggag cgggaaacgg gattcggacc cgcgacagcc
     4321 tgcttggaag gcaggggctc taccgctgag ctattcccgc cagtgcaatg gtgggcaggg
     4381 agggattcga acccccgtag gcattgccaa cagatttaca gtctgccgcc attaaccgct
     4441 cggccaccta cccacactgg agccgacgac ccgaattgaa cgggtgacca gttgattaca
     4501 aatcaaccgc tctaccagct gagctacgtc ggcctgcatc gcagttagta taggtgcaaa
     4561 gccatccctg tcaagctgca gagcggggaa cgcctatgac agcgctttcg ccagctttcg
     4621 cttcgccctc gctaaggcat tcgaaacgga cgccttcgtc atcttcagcc ctgccgcgat
     4681 gacatcgatt gtctcgccgc gcaaccgtct ctcgaagcac gcacgttcgc aatcgctgag
     4741 tgtgattgtg agcgccgcca tgtcaggggg tccggcctca gcaggatcgc ccaaggagct
     4801 tccccatcca tccagcgtgt ctgaaagctc tacatgaggc cccgaggtcc gacgtaccat
     4861 gtcgatgagt ctatgcgatg ccacagtcgc gatgtacccg tccaagctgc cacgacgaac
     4921 gtcatacgtg cgaatcgcac gcagaaagcc catgactgcc tctgccagac agtcatctcg
     4981 ttcttgccgt tcggtgcagt acttgcgggc aacgaacttg aggaggggca gatacatccc
     5041 cagcagatcc tcaaacgctg cattatcgcc catcaggtag cgccgcctca gcgattcgac
     5101 gccttctggc gagcggccgc ttctagaact cgatggcacg gaagagcaca accgccaaag
     5161 ccgtcgagac gttcagcg
//
LOCUS       DATMZD010000209         4374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_45832,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000209 DATMZD010000000
VERSION     DATMZD010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45832"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..167
                     /locus_tag="VN478_04400"
     CDS             <1..167
                     /locus_tag="VN478_04400"
                     /inference="COORDINATES: protein motif:HMM:NF036831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21944.1"
                     /translation="RAWALLHGRAFVVPDDVKTIAPYVVAHRIVRRGSRRGASHSDVN
                     HVFEQVPAPA"
     gene            164..1189
                     /locus_tag="VN478_04405"
     CDS             164..1189
                     /locus_tag="VN478_04405"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HWQ21945.1"
                     /translation="MTEKFHGATGMRLGELRFRRYHRAERLTRSGFLFFLAIVGIGAV
                     AINSGNNLLYLVFSALLSALIFSGFLSVANLRHVDVALEAADDVIMNRPARLVVRVHN
                     AGSFPLFFATVRVRNVGSDLTLAVPFVPGRSTRELPALVTMRRRGRVSGLWAELSSTF
                     PFGIVQMRLEVRVGGEVVVYPEVRPVTLQQQGERGEHRLEKARAGGDGDFFSLRRYHE
                     GDDARRIDWKVFARRGEPYVTEREARYQQETLFFLDTARAAWTGADTFEVAISKLASA
                     LTLVLETGGAFALALPGVFVDGRERTAWQRAMEALACAEFSEDHTRPAPAHSVTHDLI
                     TFVGGAV"
     gene            1186..3111
                     /locus_tag="VN478_04410"
     CDS             1186..3111
                     /locus_tag="VN478_04410"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HWQ21946.1"
                     /translation="MTGLLDLRRVLAFLLGLTACAFLGIVEPWYLLYGVAFLVALPLS
                     GRVRGRWTRRIVEALPLAWFLALVVLGTPLFALLGEAMLVAMACRFVLTDGAHDYQGL
                     VLIAVFVTVLSAAGSISVTFGLLLLTEFLLATLLLIMAQFDGPVPRIGRGFYGAVVGF
                     ALISFVFAFGVFVALPRWTLGYIKGSPALVAQTTGFSKDVTITPGQVQQDDTIVMRVE
                     PRKGAPRLSLPAYISGMRYTTFSGKQWLVAAGRPESLYASSGIDTFVVTDQQSDTLTT
                     VYLESTGTDVLFGLEHVTGVRGQFQYLRRDAEGSLFTDAPYFKTIRYDVLSLDRDTLP
                     VDSTMVHRPALLERYLQVPELSQAFRDVAARVVRGATVADKARATRDYLLANYEYSLD
                     PTATSVEDFVVNRRTGYCEHFATAMALLLRAEGVHTRLVSGFVGTEWNNASGYLIVRA
                     KDAHAWVEVLEGENWVRYDPTPSSFQQVSLITNVLDNIRMAWYRNVVTYDLARQVQTA
                     SNVNRFFARAGTAVTSAFDAFRSVLTGLVRDWRVVAGFCAAVALAFMFLRRRVEDRRG
                     RLASALESLVGERRRPGETLLELVHRAAPSRGIEDLVWRAYAVEFAGGPAPEDEKALL
                     RSIRDARRARAAADPQL"
     gene            complement(3141..>4374)
                     /gene="lpdA"
                     /locus_tag="VN478_04415"
     CDS             complement(3141..>4374)
                     /gene="lpdA"
                     /locus_tag="VN478_04415"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HWQ21947.1"
                     /translation="IPTKTMLASSKLLTRMQEARRFGLTAEGVGADFGAIMTRKDQVV
                     VRLRKGIEYLMKKNNVQVYASRATLHDATHVCVGTTGEVVEAKNVIVATGSSPVKFPP
                     FTDPGIWTSDDVFRNREQPKELVIIGGGVIGVEMATFFSAVGTKVTIVELMPHLLPAE
                     DEDVAMALTATLRKRGITIIAGAKTQSVTPSESGYTLSLDVDGVTQEVHGDHVLLSIG
                     RRSNVGPELEPLGITLSKKGIVVDDHMRTSVPGVYAVGDVKGTYMLAHVAEREGVVAA
                     ENIAGHDAHMVYSAVPSVVFTDPEISVVGKREYELKAEGIPYKAGTFPVSASGKARTM
                     EESVGFAKVLTHAETHEVLGIALYCPEATDLVMESVLAVQFGMTAERLLEAIHPHPTM
                     TEMVMEAAEGSIGLPLQL"
BASE COUNT          698 a         1371 c         1395 g          910 t
ORIGIN      
        1 tccgtgcctg ggcactcctg cacggtcggg cgttcgtggt gccggatgac gtcaagacaa
       61 ttgccccgta tgtggtggca catcgcatcg tgcggcgtgg cagccgcaga ggggcttcgc
      121 actccgatgt gaaccatgtc ttcgagcagg tgccggctcc cgcgtgacgg agaagttcca
      181 cggcgcaacg ggcatgaggc tgggtgaact gcggttcagg agataccaca gggctgagcg
      241 cctgactcgc agcgggttcc tcttcttcct tgcgatcgta ggaatcggag cggtcgccat
      301 caactcgggc aacaacctgc tgtatctggt cttcagcgcc ctgctgtccg cgctgatctt
      361 ctccggattc ctttccgtag cgaatctgcg gcacgtcgac gtggctctcg aggctgccga
      421 cgacgtgatc atgaaccgcc ccgcccggct cgtggtccgc gtgcacaatg ccgggtcgtt
      481 tccactgttc ttcgccacag tgcgtgtacg caacgtgggc agcgacctca cgcttgccgt
      541 cccgttcgtg ccaggtcggt cgacgcggga actgcctgcc cttgtgacca tgcgccggcg
      601 aggccgtgta agcgggctat gggcggaact atcctcgacg tttcccttcg gcatcgttca
      661 gatgcgcctc gaagtgcgcg tcggcgggga ggtggtcgtc tatcccgagg tccgccctgt
      721 gacgctccag cagcagggcg aacgtggaga gcaccgcctc gagaaggcca gggcaggggg
      781 cgacggagac ttcttctccc tccgtcggta ccatgagggt gacgacgcac ggcgtatcga
      841 ctggaaggtg ttcgctcgta gaggtgaacc gtatgtcacg gagcgagaag caaggtacca
      901 gcaggagacg ctgttcttcc tggacaccgc gcgtgcagcg tggactggcg ccgacacctt
      961 cgaggtcgcg atttccaagc tggcgagtgc tctcaccctg gtcctcgaga ccggaggagc
     1021 ctttgccctt gccctgccgg gagtattcgt cgacggtcgt gaacggacgg cgtggcagag
     1081 agcgatggaa gcactggcct gtgccgagtt cagcgaagat cacacacgac cggcgcctgc
     1141 gcacagcgtc acgcacgacc tgatcacatt tgtgggtggc gcagtgtgac agggctgctg
     1201 gacctcaggc gtgtgctggc gttcttgctg ggcctcacgg cttgcgcgtt tctgggcatc
     1261 gtggaaccct ggtacctgct ctacggcgtc gcgttccttg ttgccctgcc actgtccggt
     1321 cgagtgcgcg gacggtggac gaggcgtatc gtcgaggcgc ttcctctggc atggttcctc
     1381 gcacttgtcg tccttggcac gccactcttc gcattgctgg gagaggcgat gcttgttgcg
     1441 atggcatgcc gtttcgtgtt gaccgacggg gcgcatgact accagggtct ggtgctcatc
     1501 gccgtgttcg tgacggtgtt gtcggcagcc ggctccatct ctgtcacatt cggcctgctg
     1561 ctgctgacgg aattcctcct ggcgacactc ctcctcatta tggcgcagtt cgacggccct
     1621 gttcccagga tcgggcgggg cttctacgga gcagtggtgg gcttcgcgct catatccttt
     1681 gttttcgcgt tcggcgtgtt cgtcgctctc ccccgctgga ccctgggata catcaagggc
     1741 agcccggccc tggtggcaca gacgaccggg ttctccaagg acgtgaccat cacgccgggt
     1801 caggtccagc aggacgacac catcgtcatg cgtgtcgagc cccgaaaggg ggctccccgt
     1861 ttgtcactgc cggcctacat ctcgggcatg cgctacacga cattcagcgg caagcagtgg
     1921 ctggtcgcgg caggccggcc cgagtccctg tatgcatcct cggggatcga taccttcgtg
     1981 gtgacagacc agcagtccga tacgctgacc accgtatatc tcgagtccac cggcaccgat
     2041 gttctctttg gcctggaaca cgtcactggc gtgcggggac agttccagta ccttcggcgc
     2101 gacgcagagg gcagcctgtt tacggacgcg ccttacttca agacgatccg gtacgatgtc
     2161 ttgtcgctgg atcgggacac tctcccggtc gatagtacca tggttcaccg accggcgctg
     2221 ctggaacggt accttcaggt ccccgagctc tctcaggcgt tcagggacgt tgcggcacgt
     2281 gtcgtacggg gcgcgaccgt cgcggacaag gcgcgcgcga cacgcgatta cctgctggcc
     2341 aactacgagt attctctcga tcccacggcg acgtccgtcg aggacttcgt cgtcaaccgg
     2401 cgtaccgggt actgcgagca cttcgcgacg gccatggcgc tgctgctgcg cgcggaagga
     2461 gtacacaccc gcctggtgtc gggcttcgta ggcacagagt ggaacaacgc ttcaggctat
     2521 ctcatcgtgc gggccaaaga cgcgcacgcg tgggtcgaag tcctggaagg cgagaactgg
     2581 gtccgttacg atccgacacc gagttctttc cagcaggtgt cgctgatcac caacgtcctg
     2641 gacaacatcc gcatggcatg gtaccggaac gtcgtcacct atgatcttgc caggcaggta
     2701 cagacagcca gcaacgtgaa tcggttcttc gcgcgcgcag ggaccgccgt gacgtcggcg
     2761 ttcgacgcgt tccggtctgt cctgacaggg ctcgtgcgtg actggcgggt cgtcgcaggc
     2821 ttctgtgcgg ctgtcgccct ggcgttcatg ttcctgcgcc gccgggtaga ggacaggcgc
     2881 ggccgtctcg cttcagcgct cgaaagcctt gttggagaac ggcggcggcc cggtgagacg
     2941 cttcttgagc ttgttcaccg cgctgcgccg tcacgcggca tcgaggacct cgtctggcgt
     3001 gcgtacgccg tcgagttcgc cggggggccc gcgccggagg acgaaaaggc tctcctccgc
     3061 tcgatccgcg atgcccgtcg tgcccgcgcc gccgccgatc ctcaattgtg aatccccttg
     3121 gggtcaggca cttttggtat tcacaattgc agaggtaatc ctattgagcc ttctgcagcc
     3181 tccataacca tctcggtcat cgtcgggtgt ggatggatcg cctcaagaag tctctccgcc
     3241 gtcatgccga actgcacggc caggaccgac tccatgacaa ggtcggtagc ttcggggcag
     3301 tagagggcga tccccaggac ctcatgagtc tcggcatgcg tcaggacctt ggcgaagccg
     3361 acgctttcct ccatcgtcct cgccttgccg ctggcgctga cggggaacgt ccctgccttg
     3421 tacgggatcc cctctgcctt gagttcgtat tctctcttgc ccacgacgct gatctccggg
     3481 tcggtgaaga ccaccgaagg gacggctgaa tagaccatgt gagcgtcatg ccccgcgatg
     3541 ttctcggcag ccacaactcc ctctctctcg gcgacatgtg ccagcatgta cgtccccttt
     3601 acgtcgccca cggcatacac gccggggacg ctggtgcgca tatggtcgtc gacgacgatg
     3661 cctttcttcg agagcgtgat gcccagcggc tcgagttccg ggccgacgtt cgaccggcgc
     3721 ccgatagata gcaggacgtg atcgccgtgg acctcctggg tgactccgtc gacgtcgaga
     3781 ctcagggtgt agccggattc tgagggagtg acagactggg tctttgcgcc ggcgatgatg
     3841 gtgatccctc gcttcctgag cgtggctgtc agggccattg ccacgtcctc gtcctcggca
     3901 ggcaggagat gcggcatcag ctcgacgatc gtgaccttcg tcccgacggc gctgaagaat
     3961 gtggccatct cgacgccgat gacgccgccc ccgatgatga caagctcctt tggctgttcc
     4021 ctgttccgga agacatcgtc actggtccag atgccgggat cggtgaaggg agggaatttg
     4081 acgggtgagc tgccggtggc gacgatgacg ttcttcgctt cgacgacctc gccggtcgta
     4141 ccgacgcaga catgcgtcgc atcgtgcagg gtcgcgcgac tggcatagac ctgaacgttg
     4201 ttcttcttca tcaggtactc gattcccttg cgcaatcgta cgacgacctg gtccttgcgc
     4261 gtcatgatgg cgccgaagtc agccccgacg ccctcggccg tcagcccgaa cctgcgtgcc
     4321 tcctgcatgc gcgtcaggag cttactgctg gcaagcatcg tcttggtggg gatg
//
LOCUS       DATMZD010000210         3356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_116222,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000210 DATMZD010000000
VERSION     DATMZD010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116222"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="VN478_04420"
     CDS             complement(<1..608)
                     /locus_tag="VN478_04420"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HWQ21948.1"
                     /translation="MTTKPRASKHIGKNQTSIKAENTSSILRTVHETSDISRADLVRL
                     TGLTAPTVSRIVSFLVDQGVVTESPGASTGVGRKPVVLHFNGRSRYIVAIDFSWAHVR
                     TALMDLGGGILDHTERTIDPRGPIKIEFERVLETVQELLNRHRGIRVIGLGFSAPGLI
                     NSEAGVVISVPNFPSIRNLQARQLLEARFGFPTFVINDANAEA"
     gene            835..1839
                     /gene="pheS"
                     /locus_tag="VN478_04425"
     CDS             835..1839
                     /gene="pheS"
                     /locus_tag="VN478_04425"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HWQ21949.1"
                     /translation="MPSTIDEIRTALEQRLTAARDVQDVRNAQIEYLGKKGMLTALLK
                     QISGIAEDQRKQFGEQVNALKQWAVQALEQRLSDMTSTARTYDIDFSLPGQLMSAGKK
                     HILTQVEEEVESVFLSMGFDVEEGPEIEQEYYNFEALNIPADHPARDMQDTFYLAPGM
                     LLRTHTSPIQVRTMKRQDPPIRMIATGRCYRRDAVDASHTPVFTQVEGLVIDKGITFA
                     DLKGVLQEFVRGVFGSTTKVKLLPSFFPFVEPGAETAISCPICGGKGCPTCKNSGWIE
                     MGGSGMVHQNVLREVGYDTAIYQGFAFGWGIERIAMIKYGIRDIRMFYDNDLDFLGQF
                     "
     gene            1849..>3356
                     /locus_tag="VN478_04430"
     CDS             1849..>3356
                     /locus_tag="VN478_04430"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF015448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HWQ21950.1"
                     /translation="MRVKLSALHAAIDFDYSPEELVARLGDVGIEVESIERANVTFRG
                     VFVARILRAAPHPTHQKLRLIDLDLGPRGTTSICTAATNVHEGDIVPVVTPGGRTADG
                     MDITTRMFGAVESSGMLCSWKELGLDADLLSSEEKEGILRLGLGAVPGSPFEEAWPVA
                     DTGLEVSVTPDRGDALSVLGLARWIEVLKARDAHQPFDMGDIKLPLPSALPHARNDGD
                     LLVTVEDADLCSTYIGVLVQDVVSGQSSHAFRTQLYQLCVRPVSKIVDMTNVCLRQYG
                     QPTHAFDYDRLQGHRIIVRTSQPGETIVTLDGVLRVLEPETLIIADASTPVAVAGVMG
                     GLISSVTATTTRVVFEIAHFDPRAVARASRHLGIKTDAAYLYERGTDPGLTVSAVPSV
                     LAALVREQCLGSYVSRVASAGVPVPARLPLNLDVDRVNGYLGSGLEATDMQRLFGYEG
                     IRSKITGTSMSVTPPSFRGDLTSWPEYVEEVVRMEGYDEFPSHSPTLVLRRGS"
BASE COUNT          716 a          954 c          989 g          697 t
ORIGIN      
        1 gcctcggcgt tcgcgtcgtt gatgacgaac gtgggaaaac caaaacgcgc ctcaagcagc
       61 tgccgggcct gaaggttgcg aatcgatggg aagttcggaa cactgatgac gactcccgcc
      121 tctgagttga tgaggccggg ggctgagaaa cccaggccaa tgaccctgat accccggtga
      181 cgattgagca gttcctgaac ggtctccagc actctctcga actcaatctt gattggacct
      241 ctggggtcga tcgtgcgctc ggtgtgatca agaataccac cacccaagtc catgagcgca
      301 gtgcggacgt gcgcccatga gaagtcgatc gccacgatgt accgcgacct cccattgaag
      361 tgaagcacga cgggctttcg ccccacgccg gtgctggctc ccggagactc ggtgacaaca
      421 ccttgatcga ccagaaagga gacaatccgg gagaccgtcg gagcggtcaa cccggtgagc
      481 ctgaccaagt ctgcgcgtga gatatcgctc gtctcatgca ccgttcggag gatactggac
      541 gtgttctccg ccttgatgct tgtctggttc ttcccaatat gcttgctggc cctgggcttc
      601 gtggtcatgg aatctcctcg ttgcagggat atgcttggac atctgccatg aggaagtata
      661 gcagcaatcg cgcaggagtc gcgtcgttgc cagccgaact ctgcttaccc ggtatgacgc
      721 cttaagcgtg tctccaagcg actcgtgtga atcgggcacg cgcttgtaag cacgacgaat
      781 caacgtacac tttgtatgat aatcgcttga tccacactga acgaggagca gcacatgcca
      841 agcacgatag acgaaatccg tacggccctg gagcaacggc tgactgccgc tcgcgatgtc
      901 caggacgttc gaaatgcaca gatcgagtac ctgggaaaga agggtatgct gaccgcgctc
      961 ctcaaacaga tctcaggcat agctgaggat cagcgcaagc agtttggcga gcaggtcaat
     1021 gcactcaagc agtgggccgt gcaggccttg gaacagaggc tgtcagacat gacttcgaca
     1081 gcacgaacgt atgacatcga cttctctctg ccaggccagt tgatgtcggc tggcaagaag
     1141 catatcctca cacaagtgga ggaagaggtt gagtccgtct tcctttctat gggattcgac
     1201 gtagaggagg gccccgaaat cgagcaggaa tactacaact ttgaggcgct taacattccc
     1261 gcggatcatc ccgctcgtga catgcaggac acgttctacc ttgctcccgg gatgctcttg
     1321 cgcacacata catcgcccat tcaggtccgc acgatgaagc gccaggatcc acccatacgg
     1381 atgatcgcca ctgggagatg ctatcgccgt gacgccgtcg acgcgagcca tactcctgta
     1441 ttcacgcagg tcgaaggact tgttatcgac aagggtatca ccttcgcaga tctgaaagga
     1501 gtcttgcagg aattcgtgag aggcgtcttt ggctcaacca ccaaagtcaa acttctccca
     1561 tcgttcttcc cgtttgtcga acccggcgct gagacagcaa tctcgtgtcc catctgcggc
     1621 ggaaagggct gtccgacctg caagaactct ggatggatcg agatgggcgg ttcaggcatg
     1681 gtccaccaga acgtgttacg tgaggtagga tacgacaccg ccatctacca gggatttgcg
     1741 tttgggtggg gcatcgagcg gatcgcaatg atcaaatatg gcattcggga tattcgcatg
     1801 ttctatgaca atgacctgga ttttttgggc cagttctgag gtgactggat gagagtgaag
     1861 ttgtctgcgc tgcacgctgc gatcgatttc gactacagcc ccgaggagtt ggtcgcaaga
     1921 ctgggcgacg tcggcataga agtcgagtcc attgagagag ccaacgtcac gttcagaggc
     1981 gtcttcgtcg cccgaatcct gcgtgcagcg ccacacccaa cccaccagaa gcttcgactc
     2041 atagacctcg accttggacc gcgcggaacg acgagcatat gcacggctgc cacgaatgtc
     2101 catgagggtg acatcgtccc agtcgtgacg ccgggcggca ggactgcgga cggcatggac
     2161 atcacgacgc gcatgtttgg agcggtggag tcgtccggga tgctgtgttc ctggaaggaa
     2221 ctagggctcg acgctgacct cctgtcttcg gaagagaagg agggcattct gcgcctggga
     2281 ttgggggcag tccctggcag ccccttcgag gaagcatggc ctgtcgcaga cacagggttg
     2341 gaagtgagcg tgactcccga ccgcggcgat gctctgtctg tgctgggatt ggcccgatgg
     2401 atcgaagtcc tcaaggcgag agacgcgcat cagccgtttg acatgggtga catcaagctt
     2461 cccctgccgt cggcccttcc ccatgcccga aacgacggag acctgcttgt gaccgtcgaa
     2521 gacgcagacc tgtgcagtac ctacatcggt gtcctcgtgc aggatgtcgt gtcaggacag
     2581 tcgagtcacg cctttcggac tcagctctat caactgtgcg tccgccccgt gagcaagatc
     2641 gtcgacatga ccaatgtctg cctcagacag tacggacagc caacacacgc atttgactat
     2701 gatcggcttc aggggcaccg tattatcgtc cggacgtctc agcccgggga gaccattgtc
     2761 actctggacg gagtgttgcg agttctcgag ccagaaacgc tcatcatcgc tgatgcgtcc
     2821 actccagtag ccgttgccgg cgtcatggga gggctcatat ccagtgtcac ggccacgacg
     2881 actcgcgttg tcttcgaaat tgcgcacttc gatcccagag cggtcgcacg tgcgagcagg
     2941 cacctgggga tcaagacgga tgccgcgtat ctctacgagc gtggcactga tccaggactc
     3001 actgtcagcg ctgtcccttc agtcctcgca gcgctcgtcc gcgagcagtg tctcggctca
     3061 tatgtctcac gagtcgcatc tgctggagtt ccagtcccgg caagactgcc gttgaatctc
     3121 gacgtggacc gggtgaacgg ctaccttggc tcgggcctcg aagccacaga catgcagcga
     3181 ctcttcggct atgaaggcat caggtcgaag atcacaggga cgagtatgtc ggtgacgccg
     3241 ccgtcgtttc gcggtgacct gacctcgtgg cctgagtacg tcgaggaggt cgtcaggatg
     3301 gagggctacg acgagtttcc gtcccattcg ccaactctgg tactgcgtcg tggttc
//
LOCUS       DATMZD010000211         2186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_16493,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000211 DATMZD010000000
VERSION     DATMZD010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16493"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VN478_04435"
     CDS             complement(<1..212)
                     /locus_tag="VN478_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21951.1"
                     /translation="MDDDLKQKILEQIEEQHLEPTPGWRFRLVRALKHSAVAILFALT
                     ALGLAGSVYILVENGTLADLRLGPRWA"
     gene            complement(262..834)
                     /locus_tag="VN478_04440"
     CDS             complement(262..834)
                     /locus_tag="VN478_04440"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HWQ21952.1"
                     /translation="MRAQEPTDEQLVRQALTDRDAFGTLVERYERPLTSYAARLIGRQ
                     EAEDVIQQAFLQAYRNLAGFDPHLTFSAWMYRIVHNTAVNTARKHHGTVPTELELDGE
                     TVEILTGDTDVPGEVLREELEGTVSRALGQLDQRSRDVLLLSVVEGKSYSEIADILMV
                     AVNSVGPTASRAKSKLRKVLEATPPRPPTL"
     gene            1092..>2186
                     /locus_tag="VN478_04445"
     CDS             1092..>2186
                     /locus_tag="VN478_04445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper ion binding protein"
                     /protein_id="HWQ21953.1"
                     /translation="MKTRDVGITGMTCAACAVAVERSVKKLDGVISAAVNPATEKLTV
                     EYDETLVGDDALKASIVKAGYGVAEQSAAKTVVIPVRGMTCASCSAAIERALRKTPGV
                     TSASVNLATEKATVAYDPSLVRVSALKRAITAAGYTPLDVEVAASTVDEDAERKAQET
                     RSLWQRFVVSALFAIPLLYLAMGPMVPWLGWRIPAWLSPMDFPLRYALVEIALVIPSI
                     AAGYRFYIVGFRAIWHRAPNMDSLIAMGTSAAVLYSLYSTWRIAQGSFGSVEELYFET
                     AGVIITLILLGKSLESVSKGRTSQAIKKLMGLAPRTAIVVQGEQEVELPVSEVEVGDL
                     IRVRPGEKVPVDGEVMTGATSIDESMLTGES"
BASE COUNT          377 a          690 c          697 g          422 t
ORIGIN      
        1 gcccagcgcg ggccgagccg caggtcggcg agcgtcccat tctcaacgag gatgtagacg
       61 ctgcctgcca gtccgagcgc ggtcagggca aacaggatcg ccaccgcgga atgcttcagg
      121 gcccgcacaa ggcggaacct ccaccccggc gtgggctcca gatgctgttc ctcgatctgc
      181 tcgagtatct tctgcttcag gtcgtcatcc ataggtatcc tctctggatt gtgaagatca
      241 ctgtgcctgc cacgaaggga ttcacaatgt gggcggtcgt ggcggagttg cctccagcac
      301 tttccgcagc ttgctcttgg cccggcttgc ggtgggtccc accgagttga ccgcgaccat
      361 cagaatatcc gctatctcgc tgtagctctt tccttcaacc accgagagca gcaggacgtc
      421 tctgctccgc tggtccagtt gtcccagggc ccggctgacc gtaccctcga gttcctcgcg
      481 caggacctcg ccaggaacgt ccgtgtcgcc ggtcaggatc tcgaccgttt cgccgtccag
      541 ctcaagctcg gtggggacag tgccgtggtg cttgcgggcc gtgttcactg ccgtgttgtg
      601 cacgatgcgg tacatccacg cgctgaacgt caggtggggg tcgaatccgg ccagattgcg
      661 atacgcttgc agaaatgcct gctggattac gtcttccgct tcctgtcgcc cgatgagacg
      721 ggctgcatat gacgtcaggg gtcgctcgta ccgctcgacc agcgttccaa aggcatccct
      781 gtctgtgagc gcctgtcgaa cgagctgctc gtccgtcggc tcctgagcgc gcatatccat
      841 gcacctatga tacgccaaac cgcgccgttc cgtacgcgtc acattgcgga gtacagtggt
      901 actgcttcct gagggtcgaa cgacacgtca gggagacgac gcgggcacgc tgggacggca
      961 ttgcaggtgt tcacaattgc gttgttgtgg attcccatga ccggggtacg atggtggcac
     1021 acccctggag gggtggggta tcaaccgaag cgaaccaggc aagtcctggt ggcagcaact
     1081 gggaggcacg cgtgaagaca agggatgtgg gaatcaccgg catgacctgt gctgcatgcg
     1141 cagtagcggt cgagcgatcg gtgaagaagc tggacggcgt catctcggcc gcagtcaacc
     1201 cggctaccga gaagctcacc gtggagtacg acgagacgct cgtcggcgac gatgcgctga
     1261 aggcctcgat cgtgaaggcc ggctacggcg ttgccgagca gagtgccgcc aagactgtcg
     1321 tgatccctgt gcgcggaatg acgtgcgcct catgctctgc cgccatcgaa cgcgctctcc
     1381 gcaagacccc cggcgtcacc agcgcctcgg tcaacctggc gacggagaag gctaccgtcg
     1441 cgtacgatcc gtctcttgtc cgcgtgtcag ctctgaagcg cgcgatcaca gccgcggggt
     1501 atactccgct ggacgtggaa gtcgcggcga gcaccgtgga cgaggacgcc gaacgcaagg
     1561 cacaggagac acgttccctg tggcagcgct tcgtcgtctc cgccctgttt gccattcccc
     1621 tgctgtacct tgcgatgggc cccatggtgc catggctggg ctggcgcatc cccgcgtggc
     1681 tgagtcccat ggacttcccc ctgcgctatg cgctcgtcga gatcgcactg gttatcccca
     1741 gcatcgctgc gggctaccgc ttctacattg tcgggttcag ggcgatctgg caccgggccc
     1801 cgaacatgga ctccctgatc gcgatgggaa cgtccgcggc cgtcctctac agtctctatt
     1861 ctacctggcg catagcgcag ggttcgttcg gttccgtgga agagctgtac ttcgagaccg
     1921 caggcgtcat catcacgctg atcctgctgg gcaagtcact cgaatccgtc tccaaggggc
     1981 gcacgtcgca ggcaatcaag aagctgatgg gcctcgcgcc caggaccgcc atcgtcgtgc
     2041 agggcgagca ggaggtcgag ctgccggtgt ccgaggtgga ggtcggcgac ctgatccgcg
     2101 tccgtccggg cgagaaggtc ccggtcgatg gcgaggtcat gaccggcgcc acatccatcg
     2161 acgagtccat gctgacgggc gagagc
//
LOCUS       DATMZD010000212         4714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_36931,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000212 DATMZD010000000
VERSION     DATMZD010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36931"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /locus_tag="VN478_04450"
     CDS             complement(<1..791)
                     /locus_tag="VN478_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003869875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWQ21954.1"
                     /translation="MRRYFGRKLFIYILTFVLAVTIDWLIPRFMPGNPIQILISRAGL
                     KAESAQLLYSYYMKAFGLDVPVWRQYINFWIALFRGDFGISIWLFPQPVIKIIMRAVP
                     FDVLLLVPSILLSWLAGNKFGAYAARSKWLDNTISPLGYVLTATPYMWLGIMLAWFFG
                     VKMHILPFSGAYSYAMMPSLSWPFIGSLFVHWILPFASLFLVQFGGWAIGMRNMIIYE
                     LEADYSHYLEALGAPRKLVRDYAFRNAILPQITGLALQLGAIVGGA"
     gene            complement(878..2971)
                     /locus_tag="VN478_04455"
     CDS             complement(878..2971)
                     /locus_tag="VN478_04455"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWQ21955.1"
                     /translation="MRKAVVMLLVVALLAGILGSTPARAAEGIIVQITIGSTKATING
                     KEVALDQPPIIENSLTLVPFRFLGEAIGAQVAWNPVNRMVTYAYGDVSIVLIIGSTTA
                     VVNGKQVVMDVAPKILPNVGRTLVPLRFVTENLGAKVEWNGTTRMVTVTVAPTPVVFK
                     RSETLYTGGKQWGPVSNWNPFMTGNYATGTIGLVYETLFLYDPLTDVFKPWLATGGEW
                     KDATTYELKVRQGINWSDGQPLTADDVKFTIELGKKTVLNFSSLWDWLDSITKVDNYT
                     LDFKFKSALYQEWNNYLYNLPIVPQHLWASRSLTDVATGANEKPVGSGAYMYFAVDQS
                     KMVWVRNGIWWATQALGLSVAPKYIIDLVNTSNENSLGLMLQGQEDLNNNYLPGISTL
                     LHGGYAIHTYYPSAPYHIAANTAWLDVNLQHKPMDDVAFRKAIAYAINVDDIVSRDYN
                     DMVEAANPTGLLPIWDKYVDKAVVAKLGISYDPAKAKQVLAAAGYKDVDSDGFVENKD
                     GTKIALKIIVPNGWSDWMAAIQIISNSAKAVGINIVPDYPDFNGYLDARLKGTFDLAI
                     DNQVQVSNTPWSYYHFIFYDDLKNIATTQGGNYGRYNNQAAFDLVTQLDKCKADDLEG
                     MKAITSKLQTIQLTDLPIIPLWYNGMWAQMSSTYWTNWPSSAAKDNHYLPVTWNGYWN
                     MTSILMLTSLKPVTP"
     gene            complement(3385..>4714)
                     /locus_tag="VN478_04460"
     CDS             complement(3385..>4714)
                     /locus_tag="VN478_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21956.1"
                     /translation="VSRDGGATWDAVALPFGSKPMIMSIDVDPGDELRLAVAVAGGGT
                     AISKDGGRTWTTGRSGVLPSDCSVVQFVAGDDAGLAVGTQSGAVYFSTDGIGWRLALQ
                     LPEGGHVFALERSSLGLLAATSHGVFSSGDGMTWSASSTGITNLSLTGLAASPIDASA
                     LYASTDEGVYRSVDAGASWSRCSEPEAVLSILVLQDGWTVLAGTSDGSVLRSADAGDH
                     WVNVTRGIPGVKVNILRAPPAGSQVVYAGTDGGFAVSNDAGLSWEPRNVGLVATTPAG
                     SPTPRTEIAAFLPDPASPGTVLLSLLGRGFYVTTNDGVRWKPVQSSIGTPWIDSLAVD
                     GETHRYYAGTDSEGVYVSSDGWTTWSRSSSGLSTILSPTGAVNTITVGPDGTLYAGTQ
                     SRGAAISEDGGASWQRLNSGLPDLDVRRIVAAGKRVFAITAHCVVRLQTP"
BASE COUNT          961 a         1400 c         1368 g          985 t
ORIGIN      
        1 gctcctccga cgatggcccc cagctggagc gccaagccgg tgatctgggg aaggatcgcg
       61 ttgcgaaaag cgtagtccct gacgagcttt cttggcgccc ccagcgcctc gagatagtgc
      121 gagtagtctg cttcgagttc atagatgatc atgttccgca tcccgatcgc ccaaccgccg
      181 aactgcacca ggaacaggga cgcgaacggg aggatccagt gtacaaaaag gctcccgatg
      241 aacggccagg agaggctggg catcatggcg taactgtatg cccccgagaa aggaagaatg
      301 tgcatcttca ccccaaagaa ccaggccagc atgatgccga gccacatgta gggagttgcg
      361 gtcaggacgt atccgagtgg cgatatggtg ttgtcgagcc acttcgaacg tgccgcatat
      421 gcaccgaatt tgttccccgc cagccagctc agcaggatcg acgggacaag cagcagaacg
      481 tcgaacggta cggcccgcat gatgatcttg atgacgggct gcggaaacag ccatatgctg
      541 atgccaaagt ccccgcggaa cagggcaatc cagaaattga tgtactgtcg ccagacagga
      601 acgtcgagac cgaatgcctt catgtaatag gagtacagga gctgggcgga ttcagccttc
      661 aagccagccc tcgaaatgag gatctggatc ggattgccgg gcatgaagcg cggaatgagc
      721 caatcgatcg tgactgcgag gacaaacgtc aggatgtaga tgaacagttt tctaccgaag
      781 tatcgtcgca tcgtccctcc tccccgcgaa acaaggtctc agacggcaaa cgccccctcc
      841 ccttcttgag gaaagggggc gtagaatgcc tgaaccccta cggcgttacc ggtttcaacg
      901 aagtgagcat gaggatactg gtcatgttcc agtatccatt ccatgtgaca ggcagatagt
      961 ggttgtcttt ggctgccgag gaaggccagt tcgtccagta ggtgctgctc atctgagccc
     1021 acatgccgtt gtaccacaga gggatgatgg gtagatcggt gagctggatc gtctgaagct
     1081 tcgaggtgat ggccttcatg ccttccaggt cgtcagcttt gcacttgtcc agctgggtca
     1141 ccaggtcaaa agcggcctgg ttgttgtacc tgccatagtt gccgccctga gtggtcgcaa
     1201 tgttcttgag atcatcatag aagatgaagt gatagtaact ccacggggtg ttgcttacct
     1261 ggacctggtt gtcgatggcc aggtcgaatg tccctttcag gcgtgcatcc aggtacccgt
     1321 tgaagtccgg atagtcgggg acaatgttga tccccacagc cttggcgctg ttggagatga
     1381 tctggatggc tgccatccag tccgaccacc cgttgggcac gatgatcttc agcgcaatct
     1441 tggtgccgtc cttgttctcg acgaatccgt cgctgtcgac gtccttgtac ccggctgcgg
     1501 ccagtacctg cttggccttg gcggggtcat agctgatgcc cagcttcgcg acgacagcct
     1561 tgtcgacgta cttgtcccag attggcagca ggccagtcgg gttggcggcc tcgaccatgt
     1621 cgttgtagtc cctcgagacg atatcgtcca cgttgattgc gtacgcaatc gccttccgga
     1681 acgccacatc gtccatgggc ttatgctgga gattgacatc cagccaggcg gtgttggcgg
     1741 cgatatggta cggggcgctc gggtagtacg tgtggatggc gtatccacca tgaagcagcg
     1801 tggagatgcc cggcaggtag ttgttgttga gatcttcctg gccctggagc atcagcccga
     1861 gtgagttctc gttgctggtg ttgacgagat cgatgatgta cttgggagcg accgacagac
     1921 cgagcgcctg ggtcgcccac cagatgccgt ttctgaccca gaccatcttg ctttgatcga
     1981 ctgcaaagta catgtaggcg cccgagccga caggcttctc attggctccg gtggcgacgt
     2041 cggtcaggct cctgcttgcc cacagatgtt gtgggactat gggaaggttg tacagatagt
     2101 tgttccattc ctggtagagc gcactcttga acttgaagtc cagggtgtag ttgtcgactt
     2161 tggtgatcga gtccagccag tcccacagac tgctgaagtt gagcaccgtc ttcttgccaa
     2221 gctcaatggt gaacttgaca tcatcggccg tcaagggctg gccgtcggac cagttgatgc
     2281 cctgccggac cttcagttca tacgtggtgg catccttcca ttctccacct gtggcaagcc
     2341 acggcttgaa gacatctgtc aggggatcat acaggaacaa cgtctcgtag acgagcccga
     2401 tcgtccctgt cgcgtagttt cccgtcatga aggggttcca gttcgagaca ggcccccact
     2461 gctttccacc tgtgtacagt gtttctgatc gtttgaatac cacgggcgtc ggggccaccg
     2521 tgacggtgac catccttgtc gtaccgttcc actcgacctt cgcgccaaga ttctctgtaa
     2581 cgaatctcag ggggaccaat gtccttccga cgttgggaag gatcttgggt gcaacgtcca
     2641 taacaacctg cttaccgttg acgacggcag ttgttgaacc aatgatgagg acaatgctga
     2701 cgtccccata ggcgtaggtc accatcctgt tgacgggatt ccaggcgacc tgggccccga
     2761 ttgcctcccc gaggaacctg aatggtacga gcgtcaggct attctcgatg atgggtggct
     2821 gatcaagagc gacctctttg ccattgatgg tcgcttttgt agatccgatc gttatctgaa
     2881 cgataatacc ctcagcagct cttgcggggg tagatcccag gatgccagcg agcagtgcta
     2941 caacgagcag cataactact gcctttctca tcaagttacc tcctgcagac cctaccttga
     3001 ccatccggtt tgacctacag aacctcgggc attcgtcaga tcgtctctca ccccctcttt
     3061 tgcctctgtt tgactcctga catttctaat agcacattcc aaattaatgt caagctgctt
     3121 tgtgaaccag tcaagaaacc ctcgcgccgc gatagtgagc atggcagcgg acacgctctc
     3181 agacaccggg actattgtgg catgcgtgca gcagtggcaa cctggagccg ggagcacatc
     3241 gtccgaggtc tcactgtgat gtcacggacg acggtcttga gaggatcagg acttgtctcg
     3301 ccaccgaccg tgtcattggt cgcagacttc attcgcggcg aagctcgtca cggatcccaa
     3361 gcttggacct cggggtccag gcagctacgg agtctggagg cgaaccacgc agtgcgccgt
     3421 gatggcaaac acccgcttcc ctgccgcaac gatgcgcctc acgtcaaggt cgggcagacc
     3481 actgttgagg cgctgccacg aggcccctcc gtcctctgag atcgcggctc cacgcgactg
     3541 ggtccccgca tacagggtgc catccgggcc aacggtgatc gtgttgaccg ctcccgtcgg
     3601 agagaggatg gtcgacaggc cgctgcttga ccgggaccat gttgtccagc catcgctcga
     3661 aacgtacact ccctcggagt ccgtgcctgc gtagtaccga tgtgtttccc cgtcgactgc
     3721 aaggctgtcg atccaaggcg tcccaatcga cgactgcacg ggtttccagc gaaccccgtc
     3781 atttgtggtc acgtagaacc cccggcccag cagagacagc aacacagtgc ccggcgatgc
     3841 cggatccggc aggaacgcgg ctatctccgt ccgtggagtc ggcgaacctg ctggtgtggt
     3901 ggcgaccagt ccgacgtttc ttggctccca cgacaagcct gcatcgttcg agacggcgaa
     3961 ccctccgtct gtaccggcgt agaccacctg cgaccccgca gggggagccc gaaggatgtt
     4021 caccttgacg ccgggtatgc cacgcgtgac gttgacccag tgatcgccgg catcagcact
     4081 ccgcagcact gagccgtcag atgtgcccgc caggacagtc catccatcct gcaagacaag
     4141 gatactgagc acagcctctg gttcggagca gcgtgaccac gacgccccgg cgtccacgct
     4201 gcgatagacg ccctcatcgg tcgacgcgta gagagcgctt gcgtcgatcg gtgacgccgc
     4261 caatcccgtg agagaaaggt tggtgatccc tgtgctggaa gcgctccagg tcatgccgtc
     4321 accggatgag aagacaccat gtgacgttgc agccagcagg cccaggctcg agcgctccag
     4381 agcaaagacg tgtccaccct ccggcagctg cagcgcaagc ctccagccaa tgccgtccgt
     4441 cgagaagtac actgccccgg actgtgtgcc aacagcaagt ccggcatcgt cgccggcgac
     4501 gaactgcacg acactgcagt ctgacgggag tacgccgctg cgccccgtcg tccacgtccg
     4561 ccctccgtcc ttcgagatcg ctgtcccgcc gccggccacg gcgacagcca gacgcagctc
     4621 atccccggga tcgacgtcga tactcatgat catgggcttc gatccaaacg gcagagcaac
     4681 ggcgtcccat gtggcgcccc cgtcccgcga gacg
//
LOCUS       DATMZD010000213         3282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_65991,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000213 DATMZD010000000
VERSION     DATMZD010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65991"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="VN478_04465"
     CDS             complement(<1..765)
                     /locus_tag="VN478_04465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HWQ21957.1"
                     /translation="MLVTLKEVTQDALKNHYAVGAYNIHNLEYAKAVVETSYEMKAPV
                     ILQISQGSSDFAGLEELSMIARHYAAKYPIPVVVHLDHGKSFLRCVEGLRAGFSSVMF
                     DGSSLPYVENVAVTYQVVEAAHHVGVAVEAEIGKVGKSEDGATTEAEDMHYTAVDEAV
                     RFFGDTGVDALAVSIGTVHAMAVQAAHLDVELCRRLHEAMPTVPLVMHGASGAVDEDV
                     RQVIQLGITKINIATFLQKRAALAVKAMFEQKPDAIG"
     gene            complement(784..2163)
                     /gene="allB"
                     /locus_tag="VN478_04470"
     CDS             complement(784..2163)
                     /gene="allB"
                     /locus_tag="VN478_04470"
                     /EC_number="3.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR03178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0050897 - cobalt ion binding [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HWQ21958.1"
                     /translation="MQQLFQNAQTAQPDGTLLAVDILVADGVVEKFSPARGEPAPTGV
                     MDTDLHGLFVMPGGVDGHVHFDDPGFTEREDFGTGTMGAAAGGVTTIVDMPCTSLPPV
                     TTAENFRTKMKAVESKAYVDYAFWGGTTPTFIDSGAFRRLLPELKELGVVGIKAYTIS
                     GMQTYPRLDTRQMVMLFEAARDFNMLVAVHAEDYFLATYYTDKIQREGRSDPLAYKDG
                     RTYDAEPLAVSTVLALARREQTRVHIVHMSTSAGVDLVRQAKGQGIDATTETCPQYLL
                     LNREDFVRLGSLAKCTPPLRDHYDNEVLWAGLLDGTVDYVTTDHAAGIYPREKVTDNI
                     WNAYAGMPGVQLRVPLMLHEACWVRGMNLYRLGEILSTNPAKRLGLFPRKGILAVGSD
                     ADFACIDLNASMTVHAEDLYAKNKYTPFEGRELRGVVRQTFVRGNQVFDGSAKFPAGA
                     GYGKFIKST"
     gene            complement(2175..>3282)
                     /locus_tag="VN478_04475"
     CDS             complement(2175..>3282)
                     /locus_tag="VN478_04475"
                     /inference="COORDINATES: protein motif:HMM:NF024774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenine deaminase C-terminal domain-containing
                     protein"
                     /protein_id="HWQ21959.1"
                     /translation="AYIGAGISTDHECTTTDEAREKLARGMWIMMREGSVTRDVKALT
                     PLLTKENMHRIMLATDDRHPSDLLSEGHINYAVNVLLDAGVDLPTAVRLGSLNPATHY
                     SLPRKGAIAPGYWADFIIFADPHHIQPTCTYAKGELVARDGVYLGTDDGHPSRSYGIK
                     NSINIATPTYQSLQVMAEPGKQLRVLQLIPHQVVTGERLVQAKVDARGYVVSDISQDV
                     LKIAVFERHHATGRVGVAFISGFHMRQGAMASSVAHDSHNIVVCGTTDRDMLLAVQQV
                     AFIGGGIAVAGNGQVVGSLPLPYGGLMSDLSIPEVAARLDDLGKAMRDTTGCPLDDPF
                     MTLAFMALPVIPKLKITDFGLIDVEKFKVVSLFT"
BASE COUNT          625 a          967 c         1008 g          682 t
ORIGIN      
        1 cccgatggca tcgggcttct gctcaaacat tgccttgacg gcgagagcgg ccctcttctg
       61 caggaaggtc gcgatgttga tcttcgtgat gccgagctgg atgacctgcc tgacgtcctc
      121 atcgaccgca cctgatgcgc cgtgcatgac gagagggacg gtcggcatgg cttcgtgcag
      181 tctgcggcag agctctacgt cgagatgcgc cgcctgaacg gccatggcat ggactgtgcc
      241 gatggacacg gccaaggcgt cgacgccggt gtcgccgaag aatcgcactg cctcgtcaac
      301 cgcggtatag tgcatgtcct cggcctcggt ggtcgcaccg tcctcggatt tgcccacctt
      361 gccgatttcg gcctcgacgg ctactcccac atggtgcgcg gcttcgacaa cctggtaggt
      421 gacagcgaca ttctccacat agggtagcga cgaaccatcg aacatgaccg aactgaaccc
      481 ggcacgcagc ccttcgacgc agcgcaggaa gctcttcccg tgatcaaggt gcactacgac
      541 ggggatggga tacttggcag catagtgacg agcgatcatg gagagttctt ccagtccagc
      601 gaaatcggaa ctgccctgcg agatctggag gatgacaggt gccttcatct catacgacgt
      661 ctcgacgaca gccttggcat attccaggtt gtggatattg tacgcgccga ccgcatagtg
      721 gttcttgagc gcgtcttgtg tgacttcttt cagtgttaca agcattgtgc cacctccaag
      781 tggttacgtg gacttgatga attttccata gccggctccc gcagggaact tcgccgatcc
      841 gtcaaagacc tggttgccgc gcacgaaggt ctggcggacc acgcctcgca gctctcggcc
      901 ctcgaacggc gtgtacttgt tcttggcata caggtcttcg gcatgcacgg tcatcgaggc
      961 attcaggtcg atgcaggcga agtccgcatc ggaaccgacc gcaagtatgc ctttccgcgg
     1021 gaacaagccg aggcgcttgg ccgggttcgt gctcaagatt tcgccaaggc ggtacaggtt
     1081 catgccccgg acccagcagg cctcatggag catcagcgga acgcggagct gaacgccggg
     1141 catgcccgca taggcattcc agatgttgtc ggtgactttc tcccgtgggt agataccagc
     1201 tgcgtggtca gtcgtcacgt agtcgacggt accgtccagc agcccggccc agagcacttc
     1261 gttgtcatag tggtcgcgca gtgggggagt gcacttggcc agactgccca gacgtacgaa
     1321 gtcttcgcgg ttgagcaaca gatattgcgg acacgtctcc gtagtggcgt cgatgccctg
     1381 tcctttggct tgccggacga ggtccactcc agcggaagtt gacatatgga caatgtgcac
     1441 gcgcgtctgc tcgcgccggg cgagcgcaag gaccgtggat acggcgagcg gctctgcgtc
     1501 ataggtgcgt ccgtccttgt aggccagagg atccgagcgg ccctctcgct ggatcttatc
     1561 ggtgtagtac gttgcgagga agtagtcctc tgcatgtact gccaccagca tgttgaagtc
     1621 gcgcgcggcc tcgaagagca tcaccatctg gcgggtgtcc agacggggat aggtctgcat
     1681 gcccgagatc gtataggctt tgattcccac cacgcccagt tccttgagtt ccggcagcag
     1741 cctgcgaaag gctccgctgt cgatgaatgt cggggttgtg ccgccccaga acgcgtaatc
     1801 gacgtaggcc ttggactcga cagccttcat cttcgtcctg aagttctcag cggtggtcac
     1861 cggagggagg gaagtgcacg gcatgtcgac aattgtggta accccgcccg cggccgcccc
     1921 catcgttccg gtgccgaagt cctcacgttc ggtgaaaccg ggatcatcga agtggacgtg
     1981 tccgtcaact ccacccggca tgacaaagag gccatgcaag tcagtgtcca tgactccggt
     2041 cggggcaggc tcgccgcgcg ccggggagaa cttctcgacg acgccgtccg caacgaggat
     2101 atcgactgca aggagcgtgc cgtcaggttg tgctgtctga gcgttctgga agagctgctg
     2161 cattgtgaac ctccctaggt gaacaggctg acaactttga acttctcgac gtcaatcaga
     2221 ccgaagtccg tgatcttcag tttcggaatg actggaagag ccatgaaggc cagcgtcatg
     2281 aacggatcat ccagtggaca tcctgtcgtg tcgcgcatag ccttgcccag gtcatcgagg
     2341 cgggctgcga cttccggtat ggacaggtca gacatgagac cgccatatgg aagtggcaga
     2401 gagcccacaa cctgtccgtt gccagcgacg gcaatgccgc caccgatgaa ggcgacctgc
     2461 tgtacggcca gcaacatgtc tcgatcggtg gtgccgcaca cgacgatgtt gtgggaatcg
     2521 tgggcgactg acgaagccat cgccccctgc ctcatgtgga agccgctgat gaaggcaaca
     2581 cctacacgcc ccgtggcgtg gtggcgttcg aagacggcaa tcttcagcac gtcctggctg
     2641 atgtccgaca ccacgtagcc ccgcgcgtcc accttcgcct gcacgagacg ctctccggtc
     2701 actacctgat gggggatcaa ctgcaagact cgcagctgct tgcccggttc ggccatgacc
     2761 tgcaagctct ggtaggtggg agtggcaatg ttgatgctgt tcttgatacc gtagctcctg
     2821 gacggatgtc cgtcgtcggt gcccaggtag accccgtccc tggccaccag ttctcccttc
     2881 gcgtaggtac aggttggctg aatgtgatgt ggatcggcga agatgatgaa gtcggcccag
     2941 tagcccggtg cgatggcgcc cttgcgtgga aggctgtagt gagtagcggg gttgagcgaa
     3001 ccaagccgca cggcagtcgg gaggtcgacc cctgcgtcca gaagcacgtt gactgcgtag
     3061 ttgatgtggc cctccgagag cagatctgac ggatgccgat cgtcagtagc aagcatgatc
     3121 cggtgcatgt tctctttggt cagcagcggg gtcagtgcct tgacatcccg cgtaaccgat
     3181 ccctcgcgca tcatgatcca catgccccgg gctagtttct cccgcgcctc atctgtcgtc
     3241 gtgcattcgt gatcggtcga gattcctgcg ccgatatatg ct
//
LOCUS       DATMZD010000214         3606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_126460,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000214 DATMZD010000000
VERSION     DATMZD010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126460"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..1051
                     /locus_tag="VN478_04480"
                     /pseudo
     CDS             1..1051
                     /locus_tag="VN478_04480"
                     /inference="COORDINATES: protein motif:HMM:NF019155.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
     gene            1143..2270
                     /gene="prfB"
                     /locus_tag="VN478_04485"
     CDS             1143..2270
                     /gene="prfB"
                     /locus_tag="VN478_04485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HWQ21960.1"
                     /translation="MIDLEPLRKRVLECESRTTGLYQPDWLEGQEARLEALRTRTTAT
                     GFWNDQNGAAEIMGSIARIEADVASQRKALAELDDARTAIELLVGMPDPELEKEAVGS
                     IEQLEHDLQILETTAYFSGPYDASNAIVSLSSGAGGTDAQDWTEMLMRMYIRYAERQG
                     FEVLVTDYVQGEEAGIKGCTLVIRGQYAYGLLMHEKGVHRLVRISPFDANKRRHTSFA
                     AVDVIPEIPETVKDAEIPEGDLRIDVYHASGHGGQNVQKVATAVRITHIPTGISASCQ
                     NERSQLQNKRMAMAVLKSRLLVQREMQRDAQISEVRGAFKSAEWGNEIRSYVLQPYKL
                     AKDHRTGLEKGNVDAVLDGDLQDFILASVRHDAEDRRSSST"
     gene            2236..3099
                     /locus_tag="VN478_04490"
     CDS             2236..3099
                     /locus_tag="VN478_04490"
                     /inference="COORDINATES: protein motif:HMM:NF014627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HWQ21961.1"
                     /translation="MTRKIVVRAARDVVGITIGCFVYALSFALFVIPVDLAPGGVSGL
                     AIVLNHFFKVIPIGVTIMVLNAPLFLMSLKRLGKAFLWRSVYATVVSSLLIDLIVPYT
                     HGFHVDMLLSAVYAGVVMGIGIGIIFRFFGSTGGTDLLAQLLSERIGLSFGTTLLIID
                     TIIIAFSGIAFRSVTIPLYSIVSLYISAKAIDLVQEGISFSKAAWVVSNQPGSIAEKI
                     MAELDRGVTKFTAAGGFTGEPKEVVFCVVPQSQISHLKQIVHEVDPDAFVAIMDVTET
                     LGLGFKELGTR"
     gene            3099..>3606
                     /locus_tag="VN478_04495"
     CDS             3099..>3606
                     /locus_tag="VN478_04495"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWQ21962.1"
                     /translation="MIQMLSVSKRYDDGYYGLRDITFKVGPGKFVFLTGESGAGKSTV
                     LRLIYRAERPTNGTILVDGVDTAAAHGSSLTALRRRIGMVFQDYMLLFDRTVYENVAL
                     PLAVRGVGKRAASQQVDKYLDQVGLAGYQRRLCCSLSGGEKQRVALARALIAKPDIIL
                     ADEPTGNLD"
BASE COUNT          802 a         1026 c         1065 g          713 t
ORIGIN      
        1 gtaccacgag aaggaagcag agatcatcgc acaggcgggc cgcaagggat cagtcacgat
       61 cgccaccaac atggcgggcc gaggcgtcga catccttctc ggcggaaatc cggtagccct
      121 cgcgcatgct gcagctgttg ctcggatcaa ggctgatccc aagaagcagc ccgaacagta
      181 cgagaaacag tacaaggagc aatacgacac actgcttgcg gagtacaagg ggtcatggac
      241 agaggaacat gacgaggtcg tgagtttggg aggtctcttc atcctcggga ctgagcgcca
      301 tgagtcccgt cgtatcgaca accagttgcg tggacggtcc ggccggcagg gagaccccgg
      361 cgaatcgaag ttcttcctct ctgctgaaga cgacatcctg cgcatctttg gtggcgaccg
      421 catccggcac atcttcgaga tgatgaaggt cgaagagtcg acgccggtca accatccact
      481 cctgtcaaag accatcgaga tttcccagaa gaaggtcgag tcctacaact tcgatgctcg
      541 caagagcctt ctcgactacg ataacgtgct caacaagcag cgtgaggtca tctacgccga
      601 gcgcgacaag gttctggcgg gagtcgacct tgccgacgaa gtgcacgact ttatcagcga
      661 gcaggcagaa gcgactgcac aacaggtcgc tcaggcacag cgctcagaag aagtcgacat
      721 tggcaaagtc tacaatgatc ttgtcgcgga tgcttttggc aggcccgtgg agatgacgct
      781 ggcggaactg acagacctgg tcgaacgccg tgactcggag cagcagctca ctgcggcgat
      841 cagggaccgt gccgaggcca tctaccgtga gaaatacgac cggtacggag agaatgtcat
      901 gcaccagatc gagcactact tcttcctgcg caccatcgac agcgcgtgga gagatcacct
      961 cctgcagatg gacgacctta aggatggcat cggcctccgt gcatatggcc agcaggaccc
     1021 ggtcattgcc tatcagaaag agggatatga cctcttcaat cagctgatgg accagatcaa
     1081 gcaggacgtc acgcggggtc tgttcacgtt cgaactacag cccgcacaac cggagaagaa
     1141 gcatgatcga tcttgaacca ctccgcaagc gagtgcttga gtgtgagtca cgcacgacgg
     1201 gcctctatca gccggactgg ctcgaagggc aggaagcacg ccttgaggca ttgcgcacga
     1261 ggacgacggc cacaggcttc tggaatgacc agaacggggc agcagagatc atgggcagca
     1321 tcgcgcggat cgaagcggat gtcgcttccc agcggaaagc acttgcggaa ctggatgacg
     1381 cccggacggc catcgaactc cttgtcggga tgcccgatcc agagctggag aaagaggcgg
     1441 tcggcagcat cgagcagctc gagcacgatt tgcagattct tgagacgaca gcctacttca
     1501 gcggcccata tgatgcatcc aacgccattg tctcgctctc aagcggggcg gggggaacag
     1561 atgcgcagga ttggactgag atgctcatgc gcatgtacat ccggtacgcc gagcggcagg
     1621 gttttgaggt ccttgtcacg gactacgtcc agggcgagga agccggcatc aagggttgca
     1681 cgctggtcat aaggggacag tacgcctacg gattgctgat gcatgagaag ggtgttcatc
     1741 gcctggttcg catcagtcct tttgatgcga acaaacgccg tcacacgtca ttcgctgcag
     1801 tggacgtcat tccagagatc ccggagaccg tcaaggacgc cgagatcccc gaaggggacc
     1861 tgcgaataga cgtctatcat gcctcgggcc acggtggcca gaacgtgcag aaggttgcca
     1921 cagccgtgcg catcacacac attcctaccg gcatctcggc cagttgtcag aacgagcgct
     1981 cacagctgca gaacaagcgc atggcaatgg cagtcctcaa gtcgagactc cttgtgcaac
     2041 gtgagatgca gcgcgatgcc cagatctcgg aggtgcgcgg ggccttcaaa tcggccgagt
     2101 ggggcaatga gatccggtca tacgttctcc agccgtacaa gctggcaaaa gaccaccgaa
     2161 ccggtctcga gaagggaaac gtcgacgcag tcctggatgg cgacctgcag gacttcatcc
     2221 tggcaagtgt acgacatgac gcggaagatc gtcgttcgag cagcacgtga cgttgtcggc
     2281 ataacgatcg ggtgtttcgt ctacgccctt tcgtttgccc tgttcgtcat ccctgtcgat
     2341 ctggctcccg ggggtgtgtc gggccttgcc atcgtcctga accacttctt caaggtcatc
     2401 ccgatcggtg tcaccatcat ggtcctcaac gctccgctct tcttgatgag ccttaagagg
     2461 ctgggcaagg ccttcctctg gcgttccgtg tatgcaaccg tcgtctcgtc gctcctgatc
     2521 gaccttatcg tgccgtatac gcatggcttt cacgtggaca tgcttctttc agccgtgtat
     2581 gcgggggtcg tgatgggcat aggaattggc atcatcttcc gcttcttcgg ttcgaccggc
     2641 ggtacggatc tcctcgccca actgctttct gaacgcatcg gactgagctt tggaacgacc
     2701 ttgctgatca tcgatactat catcattgcg ttctcgggca tcgcgttccg gagcgtcacg
     2761 atccccctct attcgatcgt atcgctctat atctccgcaa aggcgatcga cctcgtgcaa
     2821 gaaggtatct ccttctccaa agcagcatgg gtggtcagca accagcctgg atctatcgcg
     2881 gagaagatca tggccgaact cgacagggga gtcacgaaat tcactgccgc tggcggtttc
     2941 acgggcgagc ccaaggaagt cgtgttttgt gttgtgcccc aaagccagat atctcacctc
     3001 aaacagattg tccatgaggt tgatcctgat gccttcgtgg ccatcatgga cgttaccgag
     3061 actctgggtc tcggcttcaa ggagctgggt acacgctgat gatacagatg ttgagtgtct
     3121 caaagagata cgacgacggg tactatggcc tcagggacat cacgttcaag gtgggcccgg
     3181 gaaaattcgt cttcctgaca ggcgagtcgg gtgccggcaa gagcactgtt cttcggctga
     3241 tctaccgtgc agaacggcca accaatggaa ccatcctcgt cgatggcgtg gacacagctg
     3301 cggcccatgg cagttcactg acggctctga gacggaggat cggcatggtg ttccaggact
     3361 acatgcttct gttcgaccgc acggtatacg aaaacgtcgc actgccgctc gctgttcgag
     3421 gggtgggcaa gcgggccgcc agccaacagg tagacaagta cctggaccag gtcggcctcg
     3481 caggatacca gcgtcgcctg tgctgttcgc tgtcgggagg tgagaagcag cgtgtcgctc
     3541 tcgcgcgcgc ccttatcgca aaacccgaca tcatccttgc cgacgagccg accgggaacc
     3601 tcgacc
//
LOCUS       DATMZD010000215         2155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_106528,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000215 DATMZD010000000
VERSION     DATMZD010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106528"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1116
                     /locus_tag="VN478_04500"
     CDS             <1..1116
                     /locus_tag="VN478_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21963.1"
                     /translation="RAAYVGGPGEPNWRFSLPDSMQAFGTLPGEGPAVSSDRGLWACV
                     AVGPGGRHIAMASMRSSMARVLKKPLAEVRGLSWSPDGTTLCVLAGDAAVGVISVSNT
                     VVSDPPATYSLFTVKTDGEDLVALAGPIAAVGMPRWSPTGDAVAIVEPQRGGRMTTAL
                     VMLDGNVRQVLAGSWVVLGDTAWAPDGRLLCAVRDGDGEVVPSLIDLQGGVHRLSGDW
                     QEVSTLTLAGDGSVIVSGRRNDSWCVARVAENSIDVIVSLASAAFPAVVASDRLWFVA
                     ATGNGEPGLWTCDLTGHAAQLRLTAPSMRDLHVRADGVAAAVMAGDVEREDVCVLSAQ
                     GGTIHRVGRREAIWGWAGEQTSQERDRARLWHSRRPR"
     gene            1580..>2155
                     /locus_tag="VN478_04505"
     CDS             1580..>2155
                     /locus_tag="VN478_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013265353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HWQ21964.1"
                     /translation="MRKVPMVFVALELILSLATGGCAPAPATPVDTDEVWIRRIDAGK
                     VTIVGYTGLGGAVDIPDVLGGYPVTSIDAHAFYNCTRLTSVVIPASVTSIGAYAFYDC
                     TRMISISIPTSVKSIGIHAFDDCTRLTSVVIPKSVTSIGAYTFYDCSGLTTVTIPESV
                     ARIGDHAFDDCTRLTSVVIPKSVTSIGAYTFY"
BASE COUNT          384 a          610 c          735 g          426 t
ORIGIN      
        1 cgagcagcgt acgtgggagg tcctggagag cccaactggc gattcagcct tccagatagc
       61 atgcaggctt tcgggactct gccgggagag ggtccggcag tgtcttcaga tcggggactc
      121 tgggcatgcg tcgccgtcgg acccggcggg agacacattg cgatggcgtc gatgaggtcc
      181 tccatggccc gtgtcctcaa gaagccactg gccgaggtcc gcggactgtc ctggtctccc
      241 gatgggacga cactgtgcgt actcgccgga gacgccgcgg tcggggtgat ctcggtcagc
      301 aacaccgtgg tgtcggaccc tcctgcgacg tactccctct tcacggtcaa gactgatggg
      361 gaggacctcg tcgctcttgc cggacctatt gccgccgtcg gcatgccacg gtggtccccg
      421 acaggggatg ctgtcgctat tgtcgaaccg cagcgaggcg gcagaatgac cacggccctt
      481 gtcatgctgg acggaaatgt caggcaggtg ctggccggtt catgggttgt cctcggggac
      541 acggcatggg cgcccgacgg acggttgctg tgcgcggtac gggacggcga tggcgaagta
      601 gtgccgtccc tgatcgacct tcaggggggc gtgcacaggc tctccggaga ctggcaggag
      661 gtgtcgaccc tgactctggc gggagacggc tcggtcattg tctccggacg ccggaacgat
      721 tcatggtgtg ttgcacgagt cgccgagaac tcgatcgacg tgatcgtgtc gctggcgtcg
      781 gcggctttcc ctgctgtcgt cgcatctgat cgcctgtggt ttgtcgcagc tactggcaat
      841 ggggaaccag gattgtggac ttgtgacctc acagggcacg cggcacaact cagactgaca
      901 gccccgtcga tgagggactt gcatgtacgg gcggacgggg tggctgccgc cgtcatggct
      961 ggagatgtcg agagagagga cgtctgtgtt ctgtcagccc aggggggcac gatccaccgg
     1021 gtggggcggc gcgaggcaat atggggctgg gcaggagagc agacctcaca ggagagggat
     1081 cgtgccagac tctggcactc tcgccggcca cgctgacgtg gttgcaggtg tccggatgaa
     1141 tgcaggttct gaccagccgg caggcctggt ccacgaacgc ctcggacgaa ccggatcgag
     1201 cgcttgacgc tcactggaat cggtgaatac ttcgttgtgt agacacagca tgcttggaga
     1261 tcgaccgacg cggtacccat ggttgcggag gcgggtgcag atgacaagcg aggatcgatg
     1321 gcctgacaga gtgtcggccg gccgagagag ggtggacccg ggcacctctc aagttcgtcg
     1381 aagggcttct cgcaccctcg tggccgacga ggccttcctg cgacgattct gcgtggaagt
     1441 tcccattcag ggtctccagc gagtggcttg tgacgggtga ccctctgttg tggtcggtag
     1501 tcctggtaaa tcgagtcgcg tggcgggtgt caccgttggc tgcattgatt gcccgcttga
     1561 gaagaggggg ataggcatca tgagaaaggt cccgatggtt ttcgtcgcgc tagaactcat
     1621 cctgtcgctg gcgacaggcg gctgtgcacc tgcgcctgcc accccggtgg atacggacga
     1681 ggtctggatc cgccggatcg atgccggaaa agtgacgatc gtgggctaca cggggctggg
     1741 aggcgccgtg gacatccccg atgtgcttgg tggctatcca gtgacgtcga tcgatgctca
     1801 tgcgttctac aattgcacgc gcctgaccag cgtggtcatc cccgcgagcg tcaccagtat
     1861 tggcgcttac gccttctacg attgcacgag gatgatcagc atcagcattc cgacaagtgt
     1921 gaaaagcatt ggcatccatg cgttcgacga ttgcacgaga ctgaccagcg tcgtcatccc
     1981 gaagagcgtc acgtctatcg gcgcatacac gttctacgat tgctctggac tgaccacggt
     2041 gacgatcccc gagagcgtcg ccaggatcgg tgaccatgcg ttcgacgatt gcacgagact
     2101 gaccagcgtc gtcatcccga agagcgtcac gtctatcggc gcatacacgt tctac
//
LOCUS       DATMZD010000216         3082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_75663,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000216 DATMZD010000000
VERSION     DATMZD010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75663"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            273..2243
                     /locus_tag="VN478_04510"
     CDS             273..2243
                     /locus_tag="VN478_04510"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001960.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HWQ21965.1"
                     /translation="MKLLVIPVVAGLTCIGAALYLFLRLRAAKISSPKMSEIGSYIAI
                     GVRAYLSRQLRAILLVTPIVAIVLGFTLDIWIAITFVLGVFTSLITAYMGMSASTRAN
                     VKTADDATRSMTSAFHTAVFGGSIMGFSITGFSLVVLSVLYLLFKDPNPLVGFGFGAS
                     LAALFAQIGGGIYTKAADIGADLVGKVEANIPEDDPRNPAVVADLVGDNVGDCAGRGA
                     DLFQTFSDDIITGMVVALTLVPKYGGAVLFFPMLLQSAGLAASMLGVASIRLFDRSKP
                     ETAFYFGMGVTTVFAVLGSFLVSHFLVHDMALFIGTCLGVGTTLVSSVITRYYAGSSG
                     KPVHDIAEASKRGVALNVITGLSAGLRSPLGSIVMIVIAVAAAYFISGGSLLAIVSVN
                     IGTDLLIGYIMTADAFGPITDNASGIAEMSGAHEQVVHSLSQLDSVGNTMKATTKAYA
                     MSSGTVTSFVLFSTYFSIVGLKTMNVMEPYSIAFILLGICLPYLISSLTIGSAAKTAM
                     LMVDEVRRQFRTIPGILRGEALPDYATCIDISTRNALKEMILPGVISIAVPIVVGLVF
                     GHWALGALLIGAVLSSALLGPFFNNTGTAFDNAKKLIEDSGRKGSFEHQAAVAADTVG
                     DPLKDVAGPALLIFMKLIGMAALLIAGISPLF"
     gene            2362..>3082
                     /locus_tag="VN478_04515"
     CDS             2362..>3082
                     /locus_tag="VN478_04515"
                     /inference="COORDINATES: protein motif:HMM:NF023899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006996 - organelle organization [Evidence
                     IEA];
                     GO_process: GO:0016567 - protein ubiquitination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIDE domain-containing protein"
                     /protein_id="HWQ21966.1"
                     /translation="MNAGQFISGLVLIVLSAGLYFASRSRKAHASQLNQSQTLDLQQS
                     PRGRLYGKFQGSIVADQQLTTPYSVRPAVTWSAHLEKGTEESDEDGSTTNWETVWSQS
                     ASTPFRLKGSGELWFDERGGLPNLDEPSTFDQYIHDITNPIIAPYLPGRGFLGRLSKP
                     NFHAIETSFAPGTQAFFIGPVEAVNGFWVARRGDGKEYSVLTWKSEQQVRSRMSRAST
                     VWLLLSLIALVAGILAIIISFS"
BASE COUNT          573 a          994 c          865 g          650 t
ORIGIN      
        1 tggcggaact gctgcccaac gagaacccgg agttcacctt ccagacattc atcaactggg
       61 cccgtttcgg caatctgctg gccttcgacg aggactcgga cgaggtatcg ctccagtagc
      121 cccctcactg gcagccctcc gcagcgccgc ggctccgaca acccggacct cgggttgaca
      181 gagcgcgtat ggcactataa taactgtttg ttggtttgct ggccgaaggg tgtcacttgc
      241 gtccctccgg gtccggactc tggaggcacg aaatgaaact acttgtcatt cccgttgttg
      301 ccggccttac ctgtattgga gctgcactct atctgttctt aaggctaaga gccgccaaga
      361 tctcgtcacc gaaaatgagc gaaatcggta gctacatagc tattggcgtg cgcgcgtacc
      421 tctctcgcca gctgagggcg atactgctcg tcactccaat cgtggcaatt gtcctgggct
      481 tcacgctgga catctggatc gccatcacgt tcgtgctcgg cgtcttcacg tcattgatca
      541 cggcctacat gggcatgagc gcttcgacac gcgccaacgt gaagaccgca gacgatgcga
      601 cccggtcgat gacgtcagcg tttcacaccg cggtctttgg cggatccatc atgggattct
      661 ccattaccgg attcagcctc gtggtcctgt cagtgctgta tctgctgttc aaggacccaa
      721 accccctcgt cggctttggg ttcggcgcca gccttgcagc cctctttgcg cagattggtg
      781 gaggtatcta caccaaggca gccgacatcg gcgctgatct cgtgggaaag gtcgaggcga
      841 atatccccga agatgatcca cgcaaccccg ctgtcgtggc agacctcgtc ggtgacaacg
      901 tcggcgattg tgccggccga ggcgcggacc tgttccagac gttctcagac gacatcatca
      961 ctggaatggt cgtcgcgctc acgctggtcc ccaagtacgg aggcgcagtc ctcttcttcc
     1021 ccatgctgct tcagtcggca ggtcttgccg cgtcgatgct gggcgtcgct tccatacggc
     1081 tcttcgatcg aagcaagcct gagacagcct tctactttgg catgggcgtg actacggtct
     1141 tcgcggtgct gggttccttc ctggtcagcc acttcctggt ccatgacatg gcgctgttca
     1201 tcggcacgtg cctgggcgtc ggaaccactc ttgtgtcttc ggtcatcacc cgctactacg
     1261 ccggttcttc cggcaagccg gttcatgaca ttgccgaggc atccaagcgc ggcgtagccc
     1321 tcaacgtcat cacagggctc tccgccggcc tgcgcagccc gctgggttcg atcgtgatga
     1381 tcgtcatcgc agtcgcggcg gcgtacttca tctccggagg ctcgctgctg gcgatcgttt
     1441 cggtcaacat cggcaccgat cttctcatcg gctatatcat gactgccgac gcttttggcc
     1501 cgatcaccga caacgcgtcc ggcatcgcgg agatgtccgg cgcacatgag caggtcgtgc
     1561 actcgctgtc acagctcgac tcagtcggca acacgatgaa agcgacgacc aaggcgtatg
     1621 cgatgtcatc cggcacggtc acatcatttg tcctgttctc gacgtacttc tccattgtcg
     1681 gcctcaagac gatgaacgtg atggagcctt attccatcgc cttcattctc cttggcatct
     1741 gcctgcccta cctcatcagt tccctcacca tcgggtcggc ggccaagacg gccatgctca
     1801 tggtcgacga ggtccgccgc cagttcagga ccatccccgg catcctcagg ggcgaggccc
     1861 tgccagacta cgcgacatgc atcgacatct cgacgcgcaa cgccctcaag gagatgatcc
     1921 tgccaggcgt catcagtatt gctgtaccga tcgtcgtggg actggtattc ggccactggg
     1981 ctctcggagc cctgctcatc ggagcggtac tcagttcggc cctgctggga ccattcttca
     2041 acaacacagg caccgcgttc gacaacgcca agaaactcat cgaggacagc gggcgcaagg
     2101 gctccttcga acaccaggcc gccgtcgctg cagacaccgt cggcgatcca ctcaaggatg
     2161 tcgcgggacc tgcactgctg atcttcatga agctcatcgg catggcagcc ctgctcatcg
     2221 caggtatttc gccgctgttc tagcacaggt acggcgccgt catcaggacg aggcgggggc
     2281 agtgatgctc ccgcctcttg catggatcct ccatgttctg gtatagtatc gccatgtcgt
     2341 gatgccagag aggagtgacc catgaatgca ggccagttca tcagcggttt ggtgctcatc
     2401 gtcctgtcag cagggctcta tttcgcatcc agatcacgca aggcacatgc cagccagctc
     2461 aaccagtcgc agacgctgga ccttcagcag tctccgcggg gacgcctcta cggcaagttc
     2521 cagggctcca tcgtcgcaga ccagcagctc acgacaccct actcggtccg cccggcggtc
     2581 acctggagcg ctcatttgga gaaggggacc gaggaatcgg acgaggacgg gtccacgacc
     2641 aattgggaga ccgtgtggtc gcaatcagca agcacgccgt tccggctgaa gggctcaggc
     2701 gagttatggt tcgacgagcg cggcggcttg ccaaacctgg acgagccgtc taccttcgac
     2761 cagtacatcc acgacatcac aaaccccatc atcgcgccgt atctgcctgg cagaggcttc
     2821 ctcggacgcc tcagcaagcc aaacttccac gccatcgaaa cgtcgttcgc gcccggcacc
     2881 caggccttct tcatcggtcc ggtcgaggcg gtcaacgggt tctgggtcgc gcgccgcggc
     2941 gacggcaagg aatacagcgt cctgacatgg aagagtgaac agcaggtccg aagccgcatg
     3001 agccgcgcca gcacggtctg gctgctgctc agcctgattg cactcgtggc tggcatcctc
     3061 gccatcatca tctcgttcag ct
//
LOCUS       DATMZD010000217         6058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_96822,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000217 DATMZD010000000
VERSION     DATMZD010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6058
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96822"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="VN478_04520"
     CDS             complement(<1..623)
                     /locus_tag="VN478_04520"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HWQ21967.1"
                     /translation="MTIRSRQGRISGRRRSRDAGPLVLILALVLALAVGSSFRQAGGR
                     STPLVLYYLDSSTLQLASTPLVEKLPALRVEQVAAVIELLRTPPAGKGLGTAVPAGFT
                     ARRATLLSGGILDVVLRTARDQLPMGFAEENGLYWQLVNSLLSLPDVHSVELSVDGRP
                     AGTFLSFVKTQRELEPNAAVLDKGQWVDLYFVTGDGRQVVERRTLPTG"
     gene            832..2172
                     /gene="dnaA"
                     /locus_tag="VN478_04525"
     CDS             832..2172
                     /gene="dnaA"
                     /locus_tag="VN478_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009591521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HWQ21968.1"
                     /translation="MEEQRSDSWQHLLEIFSKQLARPTFEMWFKNATTLHEDDTSLTI
                     GVPSEFARDWIVSRYYDVIKETIETIKGQPTTIAVEVVQQPPEETPTEPGMLQHNNSL
                     VLNRRYSFENFVVGAGNRLAFAACNAVAGNPGNVYNPLYIYGGVGLGKTHLLQGIAHH
                     LLAQDPGKKIVYTTGEKFTNEVVSSIQNARSNNAVIENFHKETRGADILLIDDIQFLA
                     GKERTQEEFFHIFNALYDAHKQIVITSDVLPKEIATLEERLTSRFEWGLIADISRPDY
                     ETKVAILKKKAELDKIDVPQEVLEYIAANIGSNIRELEGALMRVLATASLTNEEVTLA
                     FTEETLKDIIPVINKPITIDAIMQSVASYFHISVADLQAKRRSQDIAMPRQIAMYLSR
                     DLTEASLPYIGEKFGGRDHTTVIYACQKILQDMKNDDALRALLDTLVKQLKRGV"
     gene            2360..3439
                     /gene="dnaN"
                     /locus_tag="VN478_04530"
     CDS             2360..3439
                     /gene="dnaN"
                     /locus_tag="VN478_04530"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HWQ21969.1"
                     /translation="MKLTAKAEGLSKKLAQHAKVIQQKSSTFTSDAVLLSAEGTALQL
                     TSTDLTTYLVNAEPAGVTEAGRALVPFKFLKDIVSALSGELTIEGDEKKLQISSGKTH
                     YDLACYQMDTFPSIPEIKEGREYQFSLSEYSNAIDRVLPCVEHNEKRRLEFQGILLEQ
                     TKDYFRFVATDSRRLGYVQFDFKREPLRAIVPAKVFESFKPAGDVRVVITEDECLFSS
                     GDTQIVSLLIGAEFPNYNDIVPREWEYTVSVKKSELLDALRRVGAVVFGGTDVITLSF
                     KEGRLTVQGTAQDQGDARDELGYEGEAGGLAVSFFGTKLLDGISSVGTENAELHMNSA
                     VHPVVIKDQGKEDFLYLVMPHKQME"
     gene            3458..4525
                     /gene="recF"
                     /locus_tag="VN478_04535"
     CDS             3458..4525
                     /gene="recF"
                     /locus_tag="VN478_04535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HWQ21970.1"
                     /translation="MYLESLWVQGFRALTEQRIDITAPVTAVEGGNGAGKTTLLDAVY
                     FLASGKSCLGLTNAELIRDGESYFVVGGRYRTHDPVAGGSITHTSQAMFTTEGHRELR
                     IDGTVYHGFIRLVGGLRVSQFNFNSLFLVKGMPSARRQFINLLIASCDRTYLEMLSAY
                     SAVMTRKNALLRQSVSGVVDASLLDLFDEQITTLSTAIYAKRKSALAAVEQAMRHLIA
                     DGLFEPLSDVSILYDPRPISTGSIRALRDRELARHACLVGCHLDDFVLLRGKRQLRDT
                     ASLGEAKLVALLLTFAGAEYTRTVTGEYPVLLLDDLEGDLDSANLDRLMQLLGRFEQV
                     FIASFDITRLGGGLRTTSIQL"
     gene            4522..5103
                     /locus_tag="VN478_04540"
     CDS             4522..5103
                     /locus_tag="VN478_04540"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HWQ21971.1"
                     /translation="MKRTDNSMERIGEVIDEMRRTGRLDKGIKVQSLGDVWPAVQSLW
                     SERTIGAYVARNSHPAQFRNGTLTILCANPSIMQVLAEQKALIVERLNAHMGGKLVTD
                     LSFGLDNVHEVRIIRPQAASPVVQPDLDSLAVIIQLNPEQKARAEKAVQVIQNPALRL
                     SFLHAYEAWLRWDIWRHQEQQKRRKNRTTSRKY"
     gene            5368..5757
                     /locus_tag="VN478_04545"
     CDS             5368..5757
                     /locus_tag="VN478_04545"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HWQ21972.1"
                     /translation="MALIAHDRKKPEMIALCRDFQEVLAQEELVATKSTGSLIEELTG
                     LHVTRVNSGEKGGDLQIGSMIASDEIKAVVFLRDPLTAHPHEPDINAVLKICDVSNIP
                     LATNIATARLVLGHLKELQDTEESPAT"
     gene            complement(5764..>6058)
                     /locus_tag="VN478_04550"
     CDS             complement(5764..>6058)
                     /locus_tag="VN478_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21973.1"
                     /translation="GLNEASERLRNGLPQDARCVRGALMKGRVDTDGRARPDGSSLEA
                     LVFFARDGSFVYRVMIAEDAGRRWTIRMETLQRKEWWQESGALETMLSSLLLL"
BASE COUNT         1382 a         1706 c         1738 g         1232 t
ORIGIN      
        1 ccggtcggga gcgtgcggcg ttcgacaacc tggcggccgt cgccagtgac gaagtagaga
       61 tcgacccatt gccccttgtc cagcacggcg gcattgggct ccaattctct ctgggtcttg
      121 acgaacgaga ggaacgtccc tgcagggcgt ccgtcgaccg acaattcgac cgagtgtacg
      181 tccggcagac tgagtagtga gttgacgagc tgccagtaaa gtccgttttc ctcggcaaat
      241 cccatgggaa gctggtcgcg cgcagtcctc aggactacgt cgaggatccc tcccgaaagg
      301 agtgtcgccc ggcgcgcggt gaatcctgcg gggaccgccg tcccaagtcc cttgcctgca
      361 ggtggcgtac gcaggagctc aatgacagcc gccacttgct ccaccctgag cgcaggcagc
      421 ttctccacca ggggagtgct ggccagctgc agagtcgacg agtcaagata gtagaggacc
      481 agtggggtgc tccgcccgcc ggcctggcgg aagctggagc cgacggcaag agccagaacc
      541 aacgcgagga taagcaccag cggtcccgca tcccgggagc gtcgcctgcc gctgatcctg
      601 ccttgtctgc tgcggattgt catctgtggg ccttttcctg attgccgctg gtcttcattt
      661 cgtctatact atatcacaca aaacggatgc acattgcctg acgacgccct gtcattcggt
      721 cacgggaacg ggggcgtatg tgccaggcac gtgcagtacg acccacgctg tggcctggac
      781 gattctgcgt ccagggcgtg gacacctagc gcgagagcaa agggagcaca aatggaagaa
      841 cagcggtcgg attcctggca gcatctgctg gagatcttca gcaagcaact cgcgcgcccc
      901 acgttcgaga tgtggttcaa gaacgccacg actctccacg aagacgacac gtccctgacg
      961 atcggtgtcc ccagcgagtt cgccagggac tggatcgtca gccggtacta cgacgtcatc
     1021 aaggagacaa tcgagacgat caagggtcag cccacgacaa ttgccgtgga agtcgtacaa
     1081 cagccgccgg aagagacgcc gaccgagccc ggtatgcttc agcacaacaa ctccctggtc
     1141 ctcaacaggc gctacagctt cgaaaacttc gtcgtcgggg cgggaaaccg tctcgccttt
     1201 gcggcctgca acgcagtcgc aggaaaccct ggaaatgtgt acaaccctct ctacatctac
     1261 ggtggagtcg ggctgggcaa gacccacctc cttcagggca tcgcgcacca tctcctggca
     1321 caggacccgg gcaagaagat cgtctatacg acgggtgaga agtttaccaa tgaagtggtc
     1381 agttccatcc agaatgcgcg cagcaacaat gccgtcattg agaacttcca caaagagaca
     1441 cgcggggccg acatcctgct catcgacgac atccagttcc tcgcgggcaa ggaacgcacg
     1501 caggaggaat tctttcacat cttcaacgcg ttgtacgacg cccacaagca gatcgtgatc
     1561 acctctgatg tcctgcccaa ggagatcgcc acgctggagg aacgcctcac gtcgcggttc
     1621 gagtggggtc tcatcgctga catcagccgt ccggactatg agaccaaggt cgccatcctg
     1681 aagaagaagg ccgagctgga caagatcgat gtccctcagg aagtgctgga gtatatcgcc
     1741 gcgaacatcg ggagcaacat ccgtgaactc gagggtgcac tcatgcgtgt cctggcgacg
     1801 gcgtcgctca ccaacgagga ggtcacgctg gccttcacgg aggagacgct caaggacatt
     1861 atcccagtca tcaacaagcc gatcaccatc gacgccatca tgcagtccgt cgcatcctat
     1921 tttcacatct cggttgccga cctccaggcc aagaggcgca gccaggacat cgccatgccg
     1981 agacagatcg cgatgtacct gagccgggat ctcactgagg cgtcgctgcc gtatatcggt
     2041 gagaagttcg gcggccgtga ccacacgacg gtcatctacg catgtcagaa gatcctgcag
     2101 gacatgaaga acgacgacgc cctgagggcc ctgcttgaca ccttggtgaa gcagttgaag
     2161 agaggtgtat aacctgtgga gaatttcccc gggcattctc gatgtgtaca gtgttgataa
     2221 cgtgcgcaag ttatccacag gcgagtccac aggtgccaaa agccatacga ccgtcttcaa
     2281 gggacataca aacactacca accgccttat cttgatggtt atgattgaga gagatctttc
     2341 atattaaaga ggaggaagaa tgaaactcac tgcaaaggct gaaggcttga gcaagaaact
     2401 agcacagcac gccaaggtca tccagcagaa gagctcgacc ttcacatcag atgccgtcct
     2461 cctttctgca gaaggcacgg cacttcaact gacaagtact gacctgacga catacctggt
     2521 gaacgccgaa ccggctggag tcacagaagc cgggcgtgcc cttgttccgt tcaagtttct
     2581 taaagacatc gtgtcagcgt tgtctggtga gctcacgatc gaaggggacg aaaagaaact
     2641 acagatctct tctgggaaga cacactatga cctggcatgc taccagatgg atacctttcc
     2701 aagtattcct gagatcaagg aaggccggga atatcagttc agcctcagcg aatactccaa
     2761 tgctattgat cgtgtacttc catgtgtaga acataatgag aagcgccgtc tcgaattcca
     2821 gggaattctg ctggagcaga caaaagacta cttccgtttt gtggcgacag attctcggcg
     2881 tctgggatat gttcaattcg attttaagcg cgaaccgctg agagctatcg tccctgccaa
     2941 ggtctttgag tcattcaagc cggcgggtga tgttcgggtc gtcattacgg aagacgagtg
     3001 tctgttctcg tccggggata cacaaattgt gtctctcctt atcggcgcag agtttcccaa
     3061 ctacaacgac attgtgcccc gtgagtggga atacacggtg agtgtcaaga agtcggagct
     3121 gcttgacgca ttgcgccggg ttggtgctgt cgtctttggt ggaaccgacg tcatcacgct
     3181 ctcattcaag gaaggtcggc tgaccgttca gggtacagct caggatcagg gagatgctcg
     3241 agacgaactc ggttatgagg gagaggcagg aggtcttgcg gtttcattct ttgggacgaa
     3301 actccttgac ggtatttcct ctgtcggtac cgagaatgcg gaactgcaca tgaatagtgc
     3361 tgttcatcct gtggtcatca aggatcaggg caaggaggac tttctgtacc ttgtcatgcc
     3421 gcacaaacag atggagtgat cgccccctcg gagggcaatg tatctggaat cgctttgggt
     3481 acagggattc agggcgctga ctgagcagcg tatcgacatc actgcacccg ttactgccgt
     3541 ggaagggggc aatggtgccg gcaagacaac tctcctggat gccgtgtact ttcttgcctc
     3601 cgggaagtca tgtctgggac tcacaaatgc ggaactcatc agggatggtg agtcctactt
     3661 tgtcgttggc ggcaggtatc gcacacatga cccggtagct ggagggtcga ttactcacac
     3721 cagccaggcc atgttcacga cggagggaca tagggaacta cgcattgatg gcacggtcta
     3781 ccacgggttc attcgcctcg tcggcggact gcgggtgtcc cagttcaact tcaattcact
     3841 gttcctggtc aagggtatgc cttcggcgag gcgtcagttt atcaacctcc tgatcgcgtc
     3901 gtgtgatcgc acataccttg agatgctttc tgcatattct gcggtcatga cgcgcaagaa
     3961 cgccctgctg cgccagtcgg tctctggtgt cgtcgatgcg tccttgctgg acctgttcga
     4021 cgagcagatc acgacgctgt ccaccgccat ctatgccaag agaaagtcgg ctctcgcggc
     4081 cgtggaacag gcaatgagac atctgatcgc cgacggtctg ttcgaaccgc tctcggatgt
     4141 gtccatcctg tatgacccga ggcccatctc tactgggtcc atccgcgcgc tgcgcgaccg
     4201 cgaacttgcg cggcatgcat gtctggtggg gtgtcacctg gacgacttcg ttctgttgcg
     4261 ggggaagaga cagcttcggg atacggcgtc gcttggcgag gcaaaactgg tggcactgct
     4321 cctgaccttt gctggcgctg agtacacgcg cacagtcacg ggagaatatc cggttctcct
     4381 gctggacgat ctggaaggcg atctcgactc cgcgaacctc gatcggctca tgcagctgtt
     4441 ggggaggttc gagcaggtct tcattgcatc gtttgacatt acgcgcctgg gaggaggact
     4501 gaggacgacg tccatacagc tgtgaaaagg accgataaca gcatggagcg catcggagag
     4561 gtcattgacg agatgcggcg aaccggtcga ctggacaagg gcatcaaggt tcaatcactg
     4621 ggcgacgtct ggccggcggt tcagtccctg tggagcgagc gcaccatcgg cgcttacgta
     4681 gcccgcaact cgcatccggc acagttcaga aatggaaccc tgacgattct ctgtgccaat
     4741 ccctccatca tgcaggtcct ggccgagcag aaagcgctca ttgtcgaacg cctgaacgcc
     4801 cacatgggcg gcaagctcgt gactgacctg tccttcggtc tcgacaacgt tcacgaggtc
     4861 cggatcattc gaccacaggc tgcatctcca gttgtccaac cggatctcga ctcgcttgcg
     4921 gtcataatcc agctcaaccc ggagcagaag gcaagggcgg agaaggcagt acaggttatc
     4981 cagaaccccg ctttgcgact ctcgttcctg catgcctatg aagcgtggct ccgatgggat
     5041 atctggcggc atcaggaaca gcagaagcgc cggaagaatc ggaccacgtc aaggaagtac
     5101 tgacactatc cacaggcatt gtggataact cctggtgaag tggacacgtt atccacagcg
     5161 cgatccagac ttcggaaggc ccagggggag cctgtggacg gctgtgcaac gagaacccct
     5221 tatcaacagc gtgactgggc catgcccaaa attctcttcc acaggcattc acaacatcct
     5281 gtggataacc tgaagtgcga aggacgccgg agaagacgct tgttgattca cattcatgta
     5341 tggtaaaata gacacatgag aagaaaagtg gcattgatcg cgcacgaccg caagaaacct
     5401 gagatgatag cactgtgcag ggatttccag gaggtgctgg ctcaggagga gctggttgcc
     5461 acaaagagca caggcagtct cattgaagaa ttgacaggcc ttcacgttac gcgggtgaat
     5521 tccggagaga agggaggaga ccttcagatc ggatcgatga tcgcctctga cgagataaag
     5581 gcagtcgtct tcctcaggga ccctctgaca gctcatcctc atgagccgga catcaatgcc
     5641 gtgctgaaga tctgcgacgt cagcaatatc cctcttgcca ccaatatcgc gacggcaagg
     5701 ctggttctgg gacatctcaa ggagctgcag gacaccgaag agtcgcctgc gacgtagtcc
     5761 gtcctacaac aggagcaggc tggaaagcat cgtttccagt gccccgctct cctgccacca
     5821 ttcctttcgc tggagcgtct ccatgcggat ggtccaacgt cgtcctgcat cctcagcgat
     5881 catgactcga tagacgaatg atccatcccg cgcgaagaag acaagcgctt ccagagatga
     5941 accgtcggga cgtgcccgtc cgtcggtgtc cacccttccc ttcatcaagg ctccccggac
     6001 acaccgggcg tcctgaggta ggccgttgcg gagacgttca gacgcttcgt tcagaccg
//
LOCUS       DATMZD010000218         3359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_126618,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000218 DATMZD010000000
VERSION     DATMZD010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126618"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1673)
                     /locus_tag="VN478_04555"
     CDS             complement(<1..1673)
                     /locus_tag="VN478_04555"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HWQ21974.1"
                     /translation="MTKTRLSNCLIAVVLISTMVLSLSVCPRSVLAASPGTNPPDTYI
                     RAALKDAALRYNVPSAILMAIAYQESGWRQFDANGNTVIGSNATSQDIGIMQINSSGR
                     SDIDRLMTDIDYNIETGARMLDGKWKLAPGVGDRDRNVLENWYYAIWAYNGLSSTNNP
                     NTPGGRHYQDHVLALMARQVLGSDGQPLWPPVVVTLPSASIITDPPAWIPTPQPVHYG
                     DLYGGFNQGDNFRVLESPAGAVLSTSMQATAGFLVQNVGTTTWDATYQPILAVGDPVV
                     STLQGLAVSQPVLPGGTVAVTFALPSALPVGAVSMSLTMRKNGTQFGAAWLSQVTITD
                     LSATAAFPDSVVLGSVLDVPVQVSGTQQATVTPAFELRDMQGSLVDSSTLVASRYGTP
                     VGIAPLEVPGGQTGELRYFVGGTAAGLIQPGQYILVITFSAGASSVAGVSGTVPFGTI
                     AHILQVLPPAQPGLLIVDPLPLGSSVSVDGVLQPMLTPCAVSTVPGPHVIQLTEADFA
                     PFETTVDTSAVPVTLVGPALMPVAAIPVTLAPSPVNLDFGILKAGAGVTG"
     gene            complement(1826..2389)
                     /locus_tag="VN478_04560"
     CDS             complement(1826..2389)
                     /locus_tag="VN478_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004726725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HWQ21975.1"
                     /translation="MSQECFGSDTALLVVDVQNDFCPGGALPVADGDSIIPVINAWTP
                     RFSTVLASRDWHPAGSVHFASWPPHCIAGTYGAQFRTGLDTGRFLLVLDKGTGNADDG
                     YSAFDATNEDLASWLHGRGIEQLYICGLATDYCVLRTVLDALRLGFAVTVIGDAIAAV
                     EVHPGDGQAAVETMEAAGARFVSSDQS"
     gene            complement(2386..>3359)
                     /gene="pncB"
                     /locus_tag="VN478_04565"
     CDS             complement(2386..>3359)
                     /gene="pncB"
                     /locus_tag="VN478_04565"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR01513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0019357 - nicotinate nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0019358 - nicotinate nucleotide salvage
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HWQ21976.1"
                     /translation="AEGRSISDFGLRRAQGDGGMGASRACFVGGVGSTSNVLLAFEEG
                     VPVGGTMAHSWIQSFDSELEAFRAYAKMHPDDSVLLLDTYDTLRQGLPNAVIVGRELE
                     TQGHHLRGVRLDSGDLSYLSKKVRAVLDEQGLKHVQVSVSNQLDEYVIESLVDQGAPI
                     DMFGVGTRLVTGHSDGALDGVYKMVQLKGRPIIKISENPAKINIPGEKELYRYYDSGD
                     GLWLLDGLCLARGDRDPSILMHPDFDYRKTEVAGLKRESIRREIVRNGELVYKFPTLL
                     ETQEWGAQRFSLLYGEHKRFANPHAYHIGVSRELFDLRRSLIDTRSS"
BASE COUNT          611 a         1075 c         1085 g          588 t
ORIGIN      
        1 ccggtgacgc cggcccccgc cttgaggatc ccgaagtcca ggttgactgg tgatggagcg
       61 agtgtcacag ggatggcagc cacgggcatc agggccggtc cgaccagcgt cacgggaact
      121 gccgaggtgt cgactgtcgt ctcgaacggc gcgaagtcgg cctcggtcag ctggatcacg
      181 tgcgggcccg gcaccgtgga aaccgcgcat ggcgtcagca tcggctgcag caccccgtcg
      241 acagacacgg agctcccgag cggaagagga tcgacgatca gcagcccggg ttgtgcgggt
      301 ggaagtacct gcagaatgtg cgcaatcgtg ccgaacggga cggtacccga cacccccgcc
      361 acactggacg ccccggccga gaaggttatc accagtatgt actgaccagg ctggatcagt
      421 cccgcggccg tcccgccgac aaagtaccgc aactcccctg tctgcccgcc cggcacctca
      481 aggggggcga tgcccacggg agtgccgtag cggcttgcga cgagggtcga actgtcgacg
      541 agactgccct gcatgtcgcg cagctcaaag gcaggcgtta cggtcgcctg ctgggtgccg
      601 gagacctgaa cggggacatc cagcacactg ccaaggacga cgctgtcagg gaaggccgcg
      661 gtcgccgaga ggtcggtgat ggtcacctgt gacagccatg ctgcgccgaa ctgcgtgccg
      721 ttcttgcgca ttgtgagcga catgctcaca gctcccaccg gaagcgccga aggcagcgcg
      781 aaggtcactg cgaccgtgcc cccggggagg acgggctggg agaccgccaa tccctgcagg
      841 gtcgacacca ccgggtcgcc aacggccagg atgggttgat atgtggcgtc ccatgtcgtc
      901 gtccccacgt tctgcacgag gaacccggcg gtcgcctgca tgctcgtcga caacaccgca
      961 ccggcagggc tctccagcac cctgaagttg tccccctggt tgaatccgcc atacaggtcg
     1021 ccgtagtgaa cgggctgggg cgtcgggatc caggccggtg gatccgtgat gatggaggca
     1081 ctgggcaacg tgacgacgac cggcggccac agcggctgtc cgtcgctgcc gaggacctgc
     1141 cgggccatca gggccaggac atggtcctga tagtgtctgc cgccgggcgt attggggttg
     1201 ttggtggagg agaggccgtt gtatgcccag atggcgtagt accagttctc caggacgttg
     1261 cggtcgcggt cgcccacgcc gggcgcaagc ttccacttgc cgtcgagcat cctggccccc
     1321 gtctcgatgt tgtagtcgat atccgtcatg aggcggtcga tgtcgctgcg tcccgaactg
     1381 ttgatctgca tgatgccgat gtcctgtgat gtggcattgg acccgatgac cgtgttgccg
     1441 ttcgcgtcga actgacgcca cccggactcc tggtaggcga tcgccatcag gatagccgag
     1501 ggcacgttgt agcgcagagc tgcatccttg agggccgcac ggatgtatgt gtctggaggg
     1561 tttgtccccg gggacgctgc gaggacactg cgaggacaaa cggacaagga cagcaccatg
     1621 gtcgaaatga ggacgaccgc aatcaggcag ttcgacagac gagtcttcgt caacgcttca
     1681 ttcacctcgt tccagagaga tcgacccgct gcgggcctgc gcacagccga cacggcagcg
     1741 caccgacgtt gcgcgcgcac gctgcttgga cgcacgtcac caccatcttg ttcctcctgg
     1801 cgggaaccac tgccgtcagg gccgctcagg actggtccga tgagacgaac cgggctcctg
     1861 ccgcttccat cgtctcgacg gcagcctgtc cgtcgccggg atggacctcg acggccgcaa
     1921 tagcgtcacc gatgaccgtg acggcgaagc cgaggcgcag cgcgtcgagc acggtcctca
     1981 gaacacagta gtcggtcgcc agcccgcaga tgtacagctg ttcgatgccc cgaccgtgaa
     2041 gccagctcgc cagatcctca tttgtcgcgt cgaacgcgct gtagccgtcg tcggcgttgc
     2101 cggtcccctt gtccaggaca agcaggaagc gcccggtgtc gagcccggtc ctgaactgag
     2161 cgccataggt gcctgcgatg cagtggggag gccagctcgc gaaatgaacg ctgcctgcgg
     2221 gatgccagtc acgcgatgcg aggacagtgc tgaaccgcgg cgtccaggcg ttgatgaccg
     2281 ggatgataga gtcaccatcg gcgacaggga gagctcctcc ggggcagaag tcgttctgga
     2341 catcgacgac gagcagagcc gtgtcggaac cgaagcactc ctggctcatg acgaccgtgt
     2401 gtcgatcagg ctgcgccgga ggtcgaacag ttcacggctc acgccgatat ggtaggcatg
     2461 cgggttcgca aagcgcttgt gctcaccgta cagcagagag aaccgctgtg cgccccactc
     2521 ctgtgtctcg aggagcgtcg ggaacttgta taccagttcg ccgttccgga cgatctccct
     2581 gcggatgctc tcgcgcttga gtccagcgac ctcagtcttc ctgtagtcga agtcgggatg
     2641 catcaggatg gaggggtcac ggtcgccccg tgcaagacag agaccgtcga gcagccagag
     2701 accgtccccc gagtcgtaat agcggtacaa ctccttctcg cccgggatgt tgatcttggc
     2761 cggattctcg gagatcttga tgatcggcct ccctttgagc tggaccatct tgtagacgcc
     2821 atccagcgct ccgtcgctgt gccctgtcac caggcgcgtc ccgacgccga acatgtcgat
     2881 cggggctccc tggtctacca gcgactcgat cacgtactcg tcgagctggt tgctcaccga
     2941 tacctggacg tgcttcagtc cctgctcgtc gagaacagcc cgcaccttct tgctgaggta
     3001 ggagaggtcc ccggagtcca gacgtacgcc cctgaggtga tggccctgtg tctccagttc
     3061 cctgccgaca atgacggcgt tgggcagtcc ctgtctcagc gtgtcatatg tgtcgaggag
     3121 caggacagaa tcgtccggat gcatcttcgc ataggcgcgg aacgcctcca gctccgaatc
     3181 gaagctctgg atccagctgt gagccatcgt cccgcccacg gggacgccct cctcgaaggc
     3241 aagcaggacg ttggatgtcg aaccgacacc ccccacgaaa caggccctgc tggcacccat
     3301 gccgccgtct ccctgagccc gcctgagacc gaagtcggag atgctccttc cctcggccg
//
LOCUS       DATMZD010000219         4912 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_106689,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000219 DATMZD010000000
VERSION     DATMZD010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4912
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106689"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1079
                     /gene="ligA"
                     /locus_tag="VN478_04570"
     CDS             <1..1079
                     /gene="ligA"
                     /locus_tag="VN478_04570"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HWQ21977.1"
                     /translation="AWKFPPEEKETRLEDIIVSIGRTGVATPVAVLTPVEIDGSVVAR
                     ATLHNEDEVRRLGILVGDVVTVRKAGSVIPEILGPVVSSRTGSEHPFLMPAVCPVCGS
                     QLVRIEDEAATKCVNASCPAQVRERLLHWCSKGAMDIQGIGEAVVDQLVTMDLARDVA
                     DLYDLTEVQFLRLERMGPLVARKLLVHLEESKGRGLARALYALGVPQVGQVGARDLAQ
                     AFGSIEALASATPESLARVYGIGEKTAENITAFFGERHNGILVERLQRAGVRMVDAAP
                     PATQGPLSGKVFVFTGELSTIARSKAQELVRSLGAETSDTVSRKVTTLVSGSAAGSKL
                     AKARQLGIEILDEQQFLALVEQGN"
     gene            1102..1398
                     /gene="gatC"
                     /locus_tag="VN478_04575"
     CDS             1102..1398
                     /gene="gatC"
                     /locus_tag="VN478_04575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HWQ21978.1"
                     /translation="MAAIIDKNTLEHVASLARLEITPEEEGPLLADLERILGHVAELS
                     SVETSGVVSKRPAMVELRPDEPREGLSRETGLGGAPSMRDGFVVVRNVMGGDDE"
     gene            1391..2812
                     /gene="gatA"
                     /locus_tag="VN478_04580"
     CDS             1391..2812
                     /gene="gatA"
                     /locus_tag="VN478_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HWQ21979.1"
                     /translation="MSSGLPRTLEQYRHAIRAHELSAQEFLDETLQVINRTDPQVKAF
                     LHLRLDAARSDARAADPLAPLAGAPIAVKDNIMMAGERCTCGSRILEDYIAPYSATVV
                     RSLLAAGAVVPGKTNLDEFAMGSSTENSAFFATHNPADLSRVPGGSSGGSTAAVAAGF
                     ALGAIGSDTGGSVRQPAAFCGVVGFKPTYGLVSRYGLVAFGSSLDCIGPITRTVRDAA
                     MMMNVMAEHDPRDATSIHRPQVDFEAALHPTLAGKRVGIIRELQGLDVQPEIARALDA
                     AREAAVRSGASIVDVSVPHILSALSAYYIIAPAECSANLGRFDGVRYGLHEDGRDMTE
                     TYSRTRNAGFGAEVKRRILIGAFVLSAGYYDAYYKKAADVRQLITDELDTALQSADVL
                     LTPTTPTTAFKIGAVTDPLEMYKADVFTIPADLAGIPAVSFPAGRDAAGLPVGLQLMG
                     ARANDADVLSCANVLFAALGGES"
     gene            2809..4239
                     /gene="gatB"
                     /locus_tag="VN478_04585"
     CDS             2809..4239
                     /gene="gatB"
                     /locus_tag="VN478_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012446948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HWQ21980.1"
                     /translation="MTTTIGLEIHVQLATQTKMFCGCSTAFGDEPNTHVCPVCLGLPG
                     ALPTLNAEAVRLATRVAMALGCTVHEVSEFSRKNYFYPDLPKSYQITQFDRPIATGGA
                     VPIVTERGARSIRLTRAHIEEDAGKSMHAADITLGGETLVDFNRCGMPLIEIVTEPDM
                     QSGDEAYEFLRNLRRLVRWLNVSSGDMEKGAMRCDVNVSVSPDPEKRGTKVEIKNLNS
                     FRSVKRAIDYEEQRQAQCLADGTPIIMETRHFDEVQGTTSSMRSKEASNDYRYFPEPD
                     LPLLRLNPSELESVRAQMPELPWETQSRFERDFSLPPYDAALLTEERATAEFFQQTVA
                     LYDNARKVASYIGTEVARLLNERGKTLSETRLTPNALAELLALVENNTISNTASKDVL
                     PLLVDTDTTPVEAVRRLGLEQVSDASAIDGLAAEVIAENPTQVEQFRSGKDAVLGFLV
                     GQLMKKSRGKANPKLAGDTLQKLLRP"
     gene            complement(4317..4637)
                     /locus_tag="VN478_04590"
     CDS             complement(4317..4637)
                     /locus_tag="VN478_04590"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HWQ21981.1"
                     /translation="MRRLPASPDESPSLTIGLLVLTLAVPGSHSLKDKRSVLLPLFSR
                     IRRAFNVSLIEVGDRDARDTAIIAVVGVNSDKAECNTTLLAIEHEVENADDVVLDGFS
                     VQFL"
     gene            complement(4612..>4912)
                     /locus_tag="VN478_04595"
     CDS             complement(4612..>4912)
                     /locus_tag="VN478_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020615321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWQ21982.1"
                     /translation="LSGGEKVLTAAALQFALFKARPSLFYLLDEVDNALDENNVHRFA
                     RLLREQEGSQFLVVTHNRETMLAADVLYGVTMQESGVSKVVSLRLDSDEALARQS"
BASE COUNT         1025 a         1510 c         1476 g          901 t
ORIGIN      
        1 cagcctggaa gttccctccg gaagagaagg aaacacgtct cgaggacatc atcgtctcca
       61 tcggccgaac gggagtcgca acgccggtcg cggtacttac tccagtggag atcgatgggt
      121 cggtcgttgc gagagcgacc ctgcacaacg aggacgaggt caggcgcctg ggcatccttg
      181 tcggagacgt tgtgaccgtg cgcaaggcgg gttcggtgat ccccgagatc ctgggaccgg
      241 tcgtctcctc ccgcaccggc tccgaacacc ccttcctcat gcctgcggtg tgtccggtat
      301 gcggctcaca gctcgtgcgc atcgaggatg aagcagctac gaagtgtgtg aatgcttcct
      361 gtccagcaca agtgcgagag agactgctcc actggtgctc gaagggcgcc atggatatcc
      421 agggcatcgg agaagctgtc gtcgaccagt tggtgacaat ggatcttgca cgcgacgtag
      481 ccgacctgta tgacctgaca gaggtgcagt tcctccgcct agagcggatg ggaccccttg
      541 tggcccgcaa acttctggtc catctcgaag agagcaaggg ccgaggactc gcccgtgcgc
      601 tgtatgccct gggtgttcct caggtcggtc aggttggcgc tcgcgatctg gctcaggcct
      661 tcggctctat cgaggccctt gcatcagcta ctcccgagtc cctggcccgc gtctacggca
      721 tcggagagaa gaccgctgag aacatcacgg ctttcttcgg ggaacggcac aatggtatcc
      781 tcgtcgaacg cctccaaagg gcaggtgttc gcatggtcga cgcagcaccc cccgccacac
      841 aaggacctct cagcggcaag gtcttcgtat tcacaggcga actctctacg atagcccgca
      901 gcaaggccca ggaactcgtg cgctctctcg gagccgagac ttcggacacc gtgtcacgca
      961 aggtgacgac gctcgtctca ggatccgccg cggggagcaa acttgccaag gcccggcaac
     1021 ttggtatcga gatccttgac gaacagcagt tcctcgcgct cgttgagcag gggaactgag
     1081 gaaatctgta caggaggcaa catggcagcg atcatcgaca agaacaccct tgaacatgtg
     1141 gcttcgctcg cacgcctcga gataacgccg gaggaggaag gtcctcttct ggcagatctc
     1201 gaacgaatcc tgggtcatgt tgcagagctg agttccgttg agacttcggg cgtcgtcagc
     1261 aagcggccgg caatggtcga actccgacct gatgagccac gtgagggact ctcgcgggaa
     1321 acagggcttg gtggagcacc ctcgatgcgc gatggatttg tggtcgtacg gaatgtcatg
     1381 ggaggggatg atgagtagcg ggcttccgcg caccctggaa cagtatcgcc acgccattcg
     1441 cgcgcatgag ctatcagcac aggagttcct ggacgagacg ctgcaagtca tcaacaggac
     1501 agatcctcaa gtcaaggcgt ttctgcacct gcgcctcgat gcggctcgat cggatgcacg
     1561 cgccgccgac ccgctggccc cgcttgctgg agccccgatc gcagtcaagg acaacatcat
     1621 gatggccggg gagcgttgca catgcggctc gcgcatcctg gaagactaca tcgcaccata
     1681 ctcagcgaca gtcgtgcgat cgctcctggc tgcgggagct gtcgtaccgg gcaagaccaa
     1741 cctcgatgaa tttgccatgg gttcctcgac cgagaactcc gcattcttcg caacccacaa
     1801 tcctgccgac ttgagccgcg tccccggtgg atcgagtggc ggctcgacgg ccgccgtcgc
     1861 cgcgggcttc gcattgggag caatcggctc cgatacgggc ggttccgtcc gacagcccgc
     1921 agcattctgt ggggttgtcg gtttcaagcc gacgtacggg cttgtctcac ggtatgggct
     1981 cgtcgccttt ggatcgagcc tcgactgtat cggtccaatc acgcgaaccg ttcgggacgc
     2041 agccatgatg atgaatgtca tggcagaaca tgatccgagg gacgcgacca gcatccacag
     2101 accccaggtg gactttgagg ccgcgctgca tcctacactg gcgggcaaac gggtaggcat
     2161 catccgcgaa ctccagggac tggatgtcca gccggagatc gcacgggccc ttgacgcggc
     2221 gcgcgaagca gccgtcagga gtggcgcttc gatcgtcgac gtcagcgtgc ctcacatcct
     2281 gtcagccctt tccgcgtact acatcatcgc gcctgccgaa tgctccgcga acctcggtcg
     2341 cttcgacggc gtccgctacg gcttgcacga ggatgggcga gacatgaccg agacatacag
     2401 ccggacgcgc aatgccggat tcggcgcgga agtgaagcgt cgtatcctca tcggggcatt
     2461 cgtgctctcg gcgggatact atgacgccta ctacaagaaa gctgccgatg tccgccagct
     2521 tatcactgac gaactcgaca cggctctgca gtcggccgac gttctgctca cgccgacgac
     2581 gccgacaaca gcgttcaaga tcggcgcagt gacagacccg ctcgagatgt acaaggcaga
     2641 tgtattcacg attccagccg acctggcggg gatccccgcc gtctccttcc ctgcgggacg
     2701 agacgcggcc ggattgcctg tcggtcttca gctcatgggt gcccgcgcga acgatgccga
     2761 cgttctctcc tgtgcaaatg tcctctttgc ggcgctggga ggtgagtcgt gacgacgacg
     2821 atcggtctcg aaatccatgt ccagctggcc acgcagacca agatgttctg cggctgcagc
     2881 acagcattcg gagatgagcc caatacacat gtctgtccag tctgtctcgg actgcccggc
     2941 gccctgccca cactgaacgc cgaagcggta cgcctggcca cgcgggttgc catggctctg
     3001 ggttgtactg tccatgaagt ctccgagttc tcgcgcaaga actacttcta cccggatctc
     3061 cccaagtcct accagattac ccagttcgac cgtccaattg cgacaggggg agcagttcct
     3121 atcgttaccg agcgaggcgc ccgcagtatc cggctgacgc gcgcgcatat cgaggaggac
     3181 gcaggcaaat ccatgcacgc tgcggacatt accctcggcg gcgagacgct ggtggacttc
     3241 aaccgctgtg gcatgcccct gatcgagatc gtcactgaac cagacatgca gagcggcgac
     3301 gaagcatacg aattcctgcg gaaccttcgt cggctcgttc ggtggctcaa cgtgtcttcc
     3361 ggcgacatgg agaagggcgc catgcgctgt gacgtcaacg tttcggtgag tcccgatccc
     3421 gaaaagcgag ggacaaaagt cgagatcaag aacctcaaca gctttcgcag cgtcaagcga
     3481 gccattgact acgaggaaca gcgccaggca cagtgccttg ccgacggcac gcccatcatc
     3541 atggagacca gacacttcga tgaagtgcag ggcacgacgt cctccatgcg cagcaaggaa
     3601 gcaagcaacg actatcgcta ctttcctgaa ccggacctcc cgcttctgcg gctgaacccc
     3661 tcagaactgg agtctgtgcg ggcacagatg cccgaactgc cgtgggagac gcagtcacgc
     3721 ttcgaacgag acttcagcct tccgccatat gatgcggcct tgctgacgga ggagcgcgcc
     3781 acagccgagt tcttccagca gacagtcgcg ttgtacgaca acgccaggaa ggttgccagc
     3841 tacatcggga ccgaggtcgc gaggctcctg aacgaacgtg gaaagacgct ctctgagacg
     3901 cgactgacac ccaacgcact cgccgagctt ctcgctttgg tcgagaacaa tacaataagc
     3961 aatactgcct cgaaggacgt tctgcctctg ctcgtggaca cagacacaac gcctgtggaa
     4021 gccgtccgaa gactcggcct ggaacaggtc tcagacgcct cggcgatcga tggacttgca
     4081 gccgaggtga tagccgagaa tccgacccag gtcgagcagt tcaggagtgg caaggacgca
     4141 gtgctcggct ttctcgtcgg acagctcatg aagaagagca ggggcaaagc gaaccccaag
     4201 ctggcgggcg acacgctgca gaagctgctg aggccctagc cggtcatcca cgacaagagg
     4261 aaatcgccca gttgtctgac cgacagctgg gcgttcgcgt tgtgtgaaca ctgcggctac
     4321 aggaactgaa cgctgaagcc atcgagaacg acgtcgtccg cgttctcgac ttcatgttca
     4381 atcgccaaca gggttgtatt gcactctgcc ttgtcgctat tgacaccgac gaccgcaatg
     4441 atcgctgtgt cacgtgcgtc cctgtctcca acctcaatga gcgagacgtt gaaggcacgg
     4501 cggatacggc tgaatagcgg caggagcact gaccgcttgt ccttgagact gtggcttccg
     4561 gggaccgcca gcgtcagtac cagcagcccg atcgtcagag aaggcgattc gtcaggactg
     4621 gcgggcaagc gcctcatcgc tgtccagccg aagcgacacg accttggaga cgccgctctc
     4681 ctgcatcgtc acgccataca ggacatccgc tgcgagcatg gtctcacggt tgtgggtcac
     4741 gaccaggaac tgcgaaccct cctgctcccg gagcaagcgc gcaaaacgat gaacgttgtt
     4801 ctcgtcgaga gcgttgtcca cctcgtcgag aaggtagaac aggctggggc gcgccttgaa
     4861 caaggcaaat tgcagagcag cggccgtgag aaccttctca ccgcctgaaa gc
//
LOCUS       DATMZD010000220         2045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_137255,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000220 DATMZD010000000
VERSION     DATMZD010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137255"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..919)
                     /locus_tag="VN478_04600"
     CDS             complement(<1..919)
                     /locus_tag="VN478_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014404819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HWQ21983.1"
                     /translation="MTSTPNNEQPQGLTTTEAQQRLKDEGFNELPTASKRGWVHILLE
                     ILKEPMFLLLLACGTLYLILGDREEAMMLLGFVFVVIGITYYQEKRTENALDALRDLS
                     SPRALVKRDGEWVRIAGRDVARGDIMMLSEGDRVPADAVLLSCTNLHADESLLTGESV
                     PVRKAEWDGAQEMDRPGGEDLPFVYSGTLVVSGQGVAEVRGTGLRTEIGKIGKALQTL
                     DTEKPPLQKEITNLVRDMGLVGVGMCVIVTVVFSLSNGNNALAWQRGFLSGLAMAMGI
                     LPEEFPVVLTIFMAMGAWRMSQNQVLTRRM"
     gene            complement(1175..1984)
                     /locus_tag="VN478_04605"
     CDS             complement(1175..1984)
                     /locus_tag="VN478_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21984.1"
                     /translation="MPFVDQEVAAQLEGAWTLELVNYAETLKRLGLRPSAATAPVGDG
                     VCVYTSPDLPVNRSAGMGMHAPVTASQMQEIERFFSDREVPAQLDLCPLADPSLLELA
                     AERGYHVLRFLDVLSMDLPSPDPLPAVPLGVEIRAVDTDEVELWAQAVASGFAATDGL
                     DESDANVVLARAAGHSPGVVCYLATVGGQPAGGGAMILHNRVAALFSSSTRLPFRGRG
                     IQNGLVAMRLKAAVSAGCTTASVLATPGSISHRNLERAGFRVAYTRATLQR"
BASE COUNT          364 a          638 c          675 g          368 t
ORIGIN      
        1 gcatgcgccg ggtcaggacc tggttctggg acatgcgcca ggcacccatg gccatgaaga
       61 tggtcaggac caccgggaat tcctcaggca ggatgcccat ggccatggca agtccagaca
      121 ggaacccgcg ctgccaggcg agtgcgttgt tgccattgct gagactgaag acgacggtaa
      181 cgatgacaca catgccgacg ccgaccagcc ccatgtcgcg gaccaggttc gtgatctcct
      241 tctgcagggg tggcttctcc gtgtccagcg tctggagggc cttgccgatc ttcccgattt
      301 cggtgcgcag acccgttccg cggacttcgg caacaccctg tccggacacg acgagtgtcc
      361 ccgaatacac gaagggcagg tcttcgccgc ctggacgatc catctcctgc gctccgtccc
      421 actcggcctt gcggacgggg accgattcac cggtgagcag cgactcgtcg gcgtgcaggt
      481 tggtgcagga aaggaggact gcatcggccg ggacgcggtc tccctccgac agcatcatga
      541 tgtcgccgcg ggcgacgtcc ctgccggcga tgcggaccca ctcaccgtcg cgcttgacga
      601 gggcacgtgg gctggaaagg tcgcgcaggg cgtccagagc gttctccgtc ctcttctcct
      661 ggtagtaggt gatgccgatg acgacaaaca cgaagcccag cagcatcatc gcctcttcgc
      721 ggtcaccaag gatgaggtac agcgtgccgc aggcgaggag caggaggaac attggctcct
      781 tgaggatctc cagcaggatg tggacccagc cgcgcttgct tgcggtggga agctcgttga
      841 acccttcgtc cttgaggcgc tgctgtgcct ctgtggtcgt cagaccctgc ggttgttcat
      901 tgttcggtgt acttgtcatg acagaaacct ccatgcaaat ggtgtggtgg gttcccgacc
      961 ttgataagga ttgactctgg caagccctgc atccagttgc gcatgcgcgg tgactggcag
     1021 gcgacctgtc aggaacatca ctatgctaag gccaaaggcc ggcggcgtca agatgaagga
     1081 agcgcgtggt cgtggatggg atgaatgccc gctctgcggg cggtgtcacc gccaatcgtg
     1141 aaagagcgcg tcaagcgtcg gcggcttgga agtgctacct ctgcagggtg gcgcgtgtgt
     1201 aggccaccct gaagccggcc ctctccaggt tgcgatgcga tatgctgccc ggcgtcgcca
     1261 gcaccgaggc ggtcgtgcac cctgcggaaa cggccgcttt caggcgcatg gcgacgaggc
     1321 cgttctggat gcctctcccg cggaatggca gcctcgtgct ggatgagaag agggcggcga
     1381 cgcggttgtg caggatcatg gcgcctccac ctgcaggctg tcctccgact gtggccaggt
     1441 agcagacgac accgggactg tgtccggcag cgcgtgcgag gacgacgttg gcgtccgact
     1501 cgtccagtcc gtcggtcgcc gcgaagcccg aggcgaccgc ctgcgcccac agttcgacct
     1561 catcggtgtc gacggcgcgg atctccacgc cgagcggcac tgcaggcaga gggtccgggg
     1621 acggcaggtc catggacagg acgtcgagga accggaggac gtgatagccg cgttctgcag
     1681 ccagttccag cagcgatggg tcggcgagag gacagaggtc gagctgagcc ggtacctcgc
     1741 gatcggagaa gaagcgttcg atctcctgca tctgacttgc ggtgaccggt gcgtgcattc
     1801 ccatcccggc ggacctgttg accgggaggt ccggcgacgt atacacgcag acgccgtctc
     1861 caaccggcgc cgtggccgcc gacggtctca ggcccaatcg cttgagtgtc tcggcatagt
     1921 tgaccagttc cagcgtccac gcgccttcca gctgagccgc aacctcctga tccacgaacg
     1981 gcatgatagc ctcccctctc ctgtcgaccg agccatgatc aggaacgcat catcgccgta
     2041 gccgg
//
LOCUS       DATMZD010000221         3785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_25952,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000221 DATMZD010000000
VERSION     DATMZD010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25952"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="VN478_04610"
     CDS             <1..576
                     /locus_tag="VN478_04610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWQ21985.1"
                     /translation="EIVRATLQAGDAFVFLAVGRLEQAKGFDLLIRAIPFVAISRRGT
                     IFLIVGDGSLRGALEESARGLANVRFLGVRDDIPDLMAAADALVLSSRWEGLPMVLLE
                     AASAGLPVIATDIGGVGEVVADQETGLLVPAGDVDALGAAMIRLAGFPSEVRHDMGKQ
                     AHRLVEERYSLAAVVDTWVELYAGCADRRRT"
     gene            581..>3785
                     /locus_tag="VN478_04615"
     CDS             581..>3785
                     /locus_tag="VN478_04615"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HWQ21986.1"
                     /translation="MSDVISRKTGTRHVRLTPAQEALWLAEQMNPGTSLRHVCGAWEL
                     TGSVDLRSLRDALVEVVRRHPVMSASIEVVDGMPFQVITDRGEVPLQIVDCASGTDRE
                     TCDDAMRRTVSSVLHRAFDFRSGPLARVALVRRDDAHGILCVAAHHVIWDGLSTDVFA
                     REMSSCYASMLGGTALEPAGTGTSFLDLAATAADGLSAAQETDLEWWKDNLTPLPDAL
                     VLPSGLTRAVGPVLAMAVSSVLDHELSARVRQASRAEHATPFMTMFAAFAALLFRLTG
                     QKDMTVAVPASGRTGPDSERMIGLFVQTLPIRISVKPTVCFSELIRRVRGATLDAMAH
                     QALPYPALVRELAPERHEATDPLCQVAFQLRRHVTGLLVLPGVRVDEYDVDAPASAYG
                     LTMTIDDTGDLMTCSISFDPAAFDADMVQHLLRRYKLLLDTSTRVPGTRLEDLSILGE
                     GEEDMMLDEWGGRRRSGSVPALTAHAVVRAYARARPDAVAVEEGDRALTYAELDAKAD
                     RLALVLRAHGVSCGTVVGLLMGRSTLTVVAMLATLKAGAVYMPMDPDYPPARLSTMVR
                     VARPVLVLALEPHVDLAEGVPVMALDACLAEAMLPSSTEAEDAGSGPEDPAYVIFTSG
                     STGEPKGVVVPHRGILQLVSDHEYWDGDESDCVAHLSTVAFDAATFEVWGTLCRGARL
                     VVIDRDTVLSPDLLALTLERHGVTTAFLTTSLFNMLVSEAPGLLGRLRVVLFGGEMAD
                     PAAVRILLESGPPSRLLHVYGPTETTTFALWYEVHDVPDAAVTVPVGRPLAGATAYVV
                     DSAGQLAAPGTTGELYIGGAGVALGYVGDEQLTSQRFLPDPWTTVRGRCLYRTGDRAI
                     WRSDGNLELKGRLDGQVKLRGFRIEPGEIVAALERQENVQRAVVIVREDRPGDRRLVS
                     YVVSVDGQPLQSAVLAASLAESLPPFMLPSAYVQVESLPMTLAGKLDVRSLPAPGLPA
                     QAGAPAEPSSEAERLLADVWRRVLGLESVGIDDDFFELGGHSLTAVRLVSEVERLMGI
                     RIPMSTLFDAPTIRRLARLLSTDGWSPQWSCLVPVCST"
BASE COUNT          591 a         1129 c         1294 g          771 t
ORIGIN      
        1 gagatcgtac gcgcaaccct gcaagcaggc gatgcgtttg tcttcctggc tgttggccga
       61 cttgagcagg cgaagggctt cgacctgctg atccgggcga tcccgtttgt ggcgatctca
      121 cgtcgcggca ccatcttcct catcgtgggc gacgggtcgc tcaggggagc cctcgaggag
      181 tctgcgcgcg gccttgcgaa cgtgcgtttc ctgggcgtcc gggatgatat cccggatctg
      241 atggcggcgg cagacgcatt ggttctttcg tcgcggtggg aagggcttcc gatggtcctc
      301 ctggaggcgg cgtcggctgg tcttcctgtc attgcgacgg acatcggtgg agtcggcgag
      361 gtggtcgctg accaggagac cggccttctc gtgcctgcag gtgacgtaga cgccctaggc
      421 gccgccatga ttcgtctggc cgggttccca tcggaggtgc ggcacgatat ggggaagcag
      481 gcgcatcgac tcgtcgagga gcggtactcg cttgcggctg tcgtcgacac gtgggtggag
      541 ctgtacgcgg gttgtgcgga ccggaggcgg acgtaggact atgagcgacg tgatctccag
      601 gaagacggga acacggcatg tgcggctgac gcctgcgcag gaggcgctgt ggctggctga
      661 gcagatgaac ccgggtacca gcctccgaca tgtctgtggc gcctgggagt tgacggggtc
      721 agtagacctc cgcagtctgc gagacgcgtt ggttgaggtc gtccggcggc atccggtgat
      781 gtctgcctcc atcgaggtgg tggatgggat gccgttccag gtcatcacgg atcgtggcga
      841 agtaccgctg cagatcgtcg actgtgcctc gggcaccgac cgagagacgt gcgatgacgc
      901 catgcgtcgg actgtgtcat cggtcctcca tcgggccttc gattttcggt ccggtccact
      961 tgcccgtgtt gctcttgtgc ggcgcgacga cgctcacggt atcctctgtg tcgcggcgca
     1021 tcatgtcatt tgggacgggc tgtcgacgga cgtgttcgct cgggagatga gctcgtgcta
     1081 tgcgtctatg ctgggtggga ctgcgctcga accggccggg actgggactt cattcctcga
     1141 cttggccgcc acagctgcag atggactgag tgccgctcaa gagaccgatc tcgagtggtg
     1201 gaaggataac ctcacgcctc tgcccgacgc gcttgttctg ccctcagggc tcactcgcgc
     1261 cgtcggtcca gtcctggcga tggctgtgtc gagcgtcctg gaccacgagt tgtcggcgcg
     1321 ggtacgccag gcctcccgcg ccgagcatgc gacccctttc atgacgatgt ttgcagcatt
     1381 cgctgctctc ctgtttcggc tcaccggtca gaaagacatg accgtcgcag tgccggcgag
     1441 cggacgcacc gggccagatt ctgagcgaat gatcgggctc ttcgtgcaga cgttgcccat
     1501 ccgtatatcg gtgaagccca cggtgtgttt ctctgaactc atacgccgcg ttcgcggggc
     1561 aacgctggac gcgatggccc accaggctct gccatatccc gctctcgtgc gggagttggc
     1621 tcccgaacgt cacgaggcga cggatccact gtgtcaggtc gcgttccagc tgaggcggca
     1681 tgtcaccggc ctgcttgtct tgccgggcgt tcgcgtggac gagtacgatg tagacgcgcc
     1741 tgcgtcggca tacggtctca cgatgaccat cgatgacacg ggggacttga tgacgtgctc
     1801 catcagcttc gacccggcag ctttcgatgc agacatggtg cagcatctgc tgaggagata
     1861 caagttgctg ctggacactt cgaccagagt ccccgggacg cgtctcgaag acctcagcat
     1921 cctgggtgaa ggcgaagaag acatgatgct cgacgaatgg ggaggccgcc gcaggtctgg
     1981 atctgttccg gcgctcaccg ctcacgcagt ggtcagagca tacgcgcgtg cccgcccgga
     2041 cgctgttgcc gtcgaggaag gggaccgcgc gctgacctac gcagagctcg atgcgaaagc
     2101 ggatcggttg gccctcgtgc tgcgcgcgca tggtgtctca tgtggtactg tcgtcggctt
     2161 gctcatgggt cgttccaccc tcaccgtcgt ggcgatgctg gcgacgctga aggctggtgc
     2221 agtgtacatg ccaatggatc cggactatcc ccctgccagg ctgtccacga tggtccgggt
     2281 tgcgcgtcct gtactcgtgt tggcgctgga accccacgtg gatctcgccg agggcgtccc
     2341 tgtcatggcc ctggatgcct gccttgcaga agcgatgctg ccgtcgtcca cggaggccga
     2401 agacgcgggt tcggggcccg aggatcccgc ctatgtcatc ttcacttcag gatcgaccgg
     2461 tgagccaaag ggtgtcgtcg taccgcaccg ggggattctt cagctagtat cagaccatga
     2521 atactgggac ggcgacgaga gcgactgtgt agcacacctg tcgactgttg catttgacgc
     2581 agcgacgttc gaggtctggg gtactctgtg ccgtggcgct cgattggttg tgatcgaccg
     2641 ggatacagtc ctgtcacccg acctgctcgc gctgacactg gaacggcatg gcgtaaccac
     2701 ggcgttcctc accacgtcct tgttcaacat gctggtgagc gaagcccccg gcttgttggg
     2761 acgtctccgc gtcgtgcttt ttgggggcga gatggccgac ccggcagctg tccgcattct
     2821 cctggagtcg ggtccacctt cgcgcctgtt gcacgtctac ggtcccacgg aaacgaccac
     2881 gtttgccctg tggtacgagg tgcacgatgt tccagacgca gctgtcactg tgcccgtggg
     2941 cagaccactg gcaggagcca cggcctatgt cgtggacagc gcggggcagc tggctgcgcc
     3001 aggcacgacc ggcgaactgt acatcggagg tgcaggcgtg gccttgggat acgtcggcga
     3061 cgaacagttg acgtcgcagc gattcctgcc agacccctgg acgactgttc gggggcgctg
     3121 tctctaccga accggtgaca gggcgatctg gcgcagtgat gggaacctgg aactgaaggg
     3181 ccggctggac ggtcaggtca agctgagagg gttccgtatc gagcccggtg agatcgtcgc
     3241 cgcgttggag cggcaggaga acgtccagcg ggcggttgtg atcgtgcgtg aggaccggcc
     3301 tggcgaccgc cgccttgtgt catacgtggt gtcggtcgac ggacagccgc tgcagagtgc
     3361 tgtgctcgca gcttcgctgg cggagtccct gccgccattc atgctgccgt cggcatacgt
     3421 gcaggtagag tctctgccga tgacccttgc cggcaagctc gacgtccgtt cgcttcccgc
     3481 ccccggactc ccggctcagg caggggcgcc ggcagaaccg tcaagcgagg ctgaacgcct
     3541 gctggcggac gtgtggagac gcgtgctcgg cctggagagt gtcgggatcg acgacgactt
     3601 cttcgagttg ggtggccact cgctgacggc agtgcgactg gtatccgagg ttgaacgcct
     3661 gatgggaata cggattccca tgtcgacgct gttcgacgcg ccgaccatcc ggcggcttgc
     3721 ccggctgctg tcgaccgacg gctggagtcc acagtggagc tgcctcgtgc ccgtctgttc
     3781 cacgg
//
LOCUS       DATMZD010000222         2240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_66177,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000222 DATMZD010000000
VERSION     DATMZD010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66177"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VN478_04620"
     CDS             complement(<1..306)
                     /locus_tag="VN478_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21987.1"
                     /translation="MNNLRLLGRAGRADIADVVVERLVDFCSMAIRRDVGVEEHAELV
                     YMLADLLKLKSEFVVFGHAAAQERSLQEGIRALDEAGLYARPIIEVLEEVNAGRAPYK
                     "
     gene            complement(330..1313)
                     /gene="trpS"
                     /locus_tag="VN478_04625"
     CDS             complement(330..1313)
                     /gene="trpS"
                     /locus_tag="VN478_04625"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013625020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HWQ21988.1"
                     /translation="MKKRILSGMRPTGKLHLGQYLGVLQNYVKLQDEYDCFFMVADLH
                     ALTTKWNESGSIREFRRDMVLDWLGAGLDPQKSPIFCQSDVPEHAYLSTLLTMITPVS
                     WVERNPTVKEMIRDMGLKDNVNLGLLSYPVLMAADIMVYRAEAVPVGKDQVPHLEFTR
                     ELVRTFNHTYHVDLFPEPLPILNEFQMLPGLDGRKMSKSLGNDIVVSDSPELTSKRMM
                     TAYTDPLKMRKDDPGHPEAGCVIYQYHKLLQPATVAEAKTQCEAGCLGCVAHKRAVAV
                     MVSDKLAGFQERRMGFAADPLAVDRILEEGRERASLVAAETVRMAREAIGI"
     gene            complement(1321..1962)
                     /locus_tag="VN478_04630"
     CDS             complement(1321..1962)
                     /locus_tag="VN478_04630"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HWQ21989.1"
                     /translation="MQGLLSYIRNEIPIWIVQVPVILIVITIHEYFHAWTAWKLGDTT
                     PVETGRLSLNPLRHLDPLGTIALLLFHVGWARPVMIDFTSFKHPRRDTVLVSLAGPAS
                     NFVTAIVAYLLLLLVRSSAGVVTQFTYVYIVLKNLVEFSIALGVFNLLPIPPLDGSKA
                     FFALFFRRPERFLYDRAVDLYGTVILLALLWFNIIGGVMSRVLSFIFDTLLRL"
BASE COUNT          501 a          659 c          634 g          446 t
ORIGIN      
        1 cttgtagggt gccctgcccg cgttcacttc ctccaggacc tcgatgatcg gccttgcata
       61 cagtccagct tcatccagcg ctcggatgcc ttcctgcaac gatctctcct gggctgccgc
      121 atgcccgaaa acgacaaact cgctcttgag cttgaggaga tcagccagca tgtacacgag
      181 ttctgcatgc tcctcgacgc cgacatcgcg ccgaatagcc atactgcaga agtccacaag
      241 gcgctcgacc actacgtcgg caatgtcggc acgccccgcc cgtccgagga gcctgaggtt
      301 gttcacggaa tccgtcaggc cgggcacgtt cagataccta tggcctccct ggccatgcgt
      361 acagtctcgg ccgccacgag agacgctctc tcgcggcctt cctccaggat ccggtcgacc
      421 gcaagaggat cagcagcaaa gcccatccgg cgctcctgaa accccgccag cttgtctgac
      481 accatgactg cgacagcgcg cttgtgagcc acgcagccaa ggcagccggc ttcacactgg
      541 gtcttggctt ccgcaaccgt cgcaggctga agaagcttgt ggtactggta gatgacgcag
      601 ccagcctcgg gatgcccggg atcgtccttc cgcatcttca gtgggtccgt gtaagccgtc
      661 atcatgcgct tgctcgtcaa ctcaggagag tccgacacca cgatgtcgtt gccgagagac
      721 ttgctcatct tgcgtccatc aaggccgggc agcatctgga attcgttcag aatgggcaag
      781 ggttcaggga agagatcgac atgatatgta tggttgaagg ttcgtaccag ttccctggtg
      841 aactcgaggt gcggtacctg gtccttgccg acaggaaccg cctccgcacg gtacaccatg
      901 atgtctgcgg ccatcaggac aggatagctc aggagaccaa ggttcacgtt atccttgagg
      961 cccatgtccc ggatcatttc cttgactgtc gggtttcgct ctacccacga tacaggggtg
     1021 atcatcgtga ggagcgtgga cagatacgca tgctccggga catcagactg gcagaagatc
     1081 ggtgacttct gtgggtccag cccggccccc agccagtcga ggaccatatc gcgccggaac
     1141 tcgcggatcg agccgctttc gttccacttc gtggtcagcg catgcaggtc agccaccatg
     1201 aagaagcagt cgtactcgtc ctggagcttc acgtagttct gcagcacgcc caggtactgc
     1261 cccagatgca acttgcccgt cggacgcatg ccactcagga tacgcttttt cataaccacc
     1321 tcacagcctc aggagagtat caaagatgaa actgagtact ctggacatca caccaccaat
     1381 gatgttgaac cataggagag ccagcaggat gacggtgccg tacaggtcga cggcacggtc
     1441 gtacaggaac cgctccggtc tcctgaagaa cagggcgaag aatgccttgg aaccgtcaag
     1501 tggaggaatc ggaagcagat tgaacacgcc aagcgcaatg gagaactcca cgaggttctt
     1561 caggacgata tagacgtagg tgaattgtgt gactactccg gccgagctcc ttacaagcag
     1621 caggagcagg tacgcgacga tggcggtaac gaagttggat gccggtccag ccagagagac
     1681 caggacagta tcacggcgag ggtgcttgaa cgacgtgaaa tcgatcatga caggacgcgc
     1741 ccagccgaca tggaacagca ggagagcgat tgtcccaagc gggtccagat gacgcagagg
     1801 gttgaggctg agacgtccgg tctcgactgg cgtcgtgtcc cccagcttcc acgccgtcca
     1861 cgcatggaag tactcatgga tggtaatgac aatcaggata accggcacct gaacaatcca
     1921 gatgggtatc tcgttgcgga tgtagctcag aagtccctgc atgtattctg tcctttctcg
     1981 acgatggccg gtcatgccgg ctgttgttat ggagtcatag tagcccggtt tccgccttac
     2041 gcaatcgctt ggaaacgagc gattcttcat tgcggacgcc cgctttcctg ctacaatcaa
     2101 cacatgatca agatgagagt tcgatacagc aaggaagaag aggcgtgcct gctttcacac
     2161 catgaagtcc aggcagctct catacgatgg ctgcgtcgca gcgaccttcc cgtggagtac
     2221 agcgagggct tcaaccagcg
//
LOCUS       DATMZD010000223         2051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_56716,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000223 DATMZD010000000
VERSION     DATMZD010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56716"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1065)
                     /locus_tag="VN478_04635"
     CDS             complement(<1..1065)
                     /locus_tag="VN478_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21990.1"
                     /translation="MRKFLLVLLTVLIVAVSAGCSAQSTTTPSTDSTQTTTPSSSGTD
                     AAGTPPTGTPPTGTPPTGTPPSGTPPTGTSSTASTSPSYTITGVYTQTSGTQAAADKT
                     YAASKADESAVYVTKTGVLTLANATVTSTGNTSSQDNSSFHGLNAAVLAADGGTIHMT
                     RGTITTSGLGANGAFATGTSSTVTLDKTTINATGDGGHAVMATAGGSMTLSNVEMTTA
                     GKNSGAIATDRGGGIITATGCIVTAKGVDSPAIYSTGAISITGSTLLATGAESAVIEG
                     ANSITLTDSSLSSTKAGKWGVLIYQSMSGDAEGAKGTFTMTGGTLALTAASGPLFYVT
                     NSTAVITLKGVQITATSGTLI"
     gene            complement(1270..1470)
                     /locus_tag="VN478_04640"
     CDS             complement(1270..1470)
                     /locus_tag="VN478_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ21991.1"
                     /translation="MKKTALALLVIAIMAVSAGCSAQSSAAPQTQPDAATSATPQSSG
                     PIEFDALTFSAALTISGPTASA"
     gene            1652..>2051
                     /locus_tag="VN478_04645"
     CDS             1652..>2051
                     /locus_tag="VN478_04645"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWQ21992.1"
                     /translation="MPRSEQANTQIKDDRQKQILTSALRVFIHKGFAAAKMCDIATEA
                     GISYGLMYHYFQSKDEIYTELVRNAVDSSRRTIEQLRADNGEPIDKMRSLTRAIFKSV
                     DEHKSAGYSFVLMMEAMTSGVYPVQAALHSH"
BASE COUNT          365 a          594 c          683 g          409 t
ORIGIN      
        1 gatcagggtt ccggaggtcg ctgtgatctg gacacccttg agtgtgatga cagccgtcga
       61 gttggtgacg tagaacaggg gcccgctggc agcggtaagg gcgagggtgc cgccggtcat
      121 cgtgaaggtg cctttggcgc cttcggcgtc accggacatg ctctgataga tgagaacgcc
      181 ccacttgcct gccttcgtcg acgaaagact cgagtctgtc agggtgatgg agttggctcc
      241 ctcgatgacg gcagactcgg cccctgttgc gagcagcgtg ctgcctgtga tactgatggc
      301 gccggtcgag tagatggcag gggagtcgac gcccttggcg gtcacgatgc acccggtcgc
      361 ggtgatgatt ccgccgccgc ggtcagtcgc gatggcaccc gagttcttgc cggccgtggt
      421 catctccaca ttgctcaatg tcatggagcc gccggccgtc gccatgaccg catgtccgcc
      481 gtcgccggtg gcattgatcg tggtcttgtc gagcgtgact gtggagctgg tgccggtggc
      541 aaatgcgccg ttggcgccaa ggccggacgt cgtgatggtg ccgcgcgtca tatggatggt
      601 tccgccgtcg gcagccagca ccgccgcgtt caggccgtgg aagctgctat tgtcctgtga
      661 ggatgtattg ccggtcgacg tgaccgtcgc gttcgcaagc gtcaggacac cggtcttggt
      721 gacatacacg gccgactcat cggctttgga tgctgcatac gtcttgtccg ctgctgcctg
      781 cgtgccgctg gtctgtgtat atacaccagt gatggtgtag gacgggctcg tggacgccgt
      841 cgacgaagtg ccggtgggtg gcgtccctga gggaggcgta ccggtgggtg gagttcccgt
      901 cggcggtgtt ccggtcggtg gggtcccggc tgcgtccgtt ccggacgagc tgggcgtcgt
      961 ggtctgtgtg gagtcggtgc tgggggtcgt ggtcgactgg gctgagcagc cggccgaaac
     1021 ggccacgatc aggaccgtca gaagaacaag aaggaacttc ctcacctggt catctcctcg
     1081 attgaaatgc cccgacgcct ggacggtagc cgggggctga catggtttgc aggatcggcc
     1141 ccactgcttc gggcaccctc tggaggttgg ggaaggcttg tgtggggcct atgtggaaaa
     1201 gcctgtcgcc gtgcatcgtg gagagctgct cccgatgcga cagactggtc accgctcatc
     1261 atgatggact caggccgacg ccgtcggacc ggagatcgtg agtgccgcag agaaggtcag
     1321 agcgtcgaat tctatcggcc ccgacgactg gggcgttgcg ctggtcgccg catccggttg
     1381 cgtctgcggt gcggcggacg actgcgcgga gcagccggcg ctcacggcca tgatcgcgat
     1441 caccagtagc gcaagtgctg tcttcttcac aaggacatct ccttagtccg aacgcgcctg
     1501 cgtgctggga tcaggcgctc gtacttctag tacaacaggc cccggaactc cgtacacgcc
     1561 aatgtgaacg gcgctgtatt gcctagtcca tgattcgcca tacactaacc atccgttgac
     1621 tgacaagtca atcaaaagga gccgttggat tatgccgcgc agtgaacaag ccaatacaca
     1681 gatcaaggac gaccgacaga agcagatcct cacgtccgcg ttgagggtct tcatccacaa
     1741 gggcttcgcc gctgcgaaga tgtgcgacat cgcgaccgag gccgggatca gctatggact
     1801 gatgtaccac tacttccagt ccaaggacga gatctacacc gagctggtca ggaacgccgt
     1861 ggattcgtcc cggcgcacga tcgaacagct gcgagcagac aacggcgaac ccatcgacaa
     1921 gatgcggtcc ctgacgcgtg ccatcttcaa gagcgtcgat gagcacaagt cggcaggcta
     1981 ctcctttgtc ctgatgatgg aggccatgac cagcggcgtc tatccggtgc aggccgccct
     2041 gcactcgcac a
//
LOCUS       DATMZD010000224         2648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_37383,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000224 DATMZD010000000
VERSION     DATMZD010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37383"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="VN478_04650"
     CDS             <1..702
                     /locus_tag="VN478_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWQ21993.1"
                     /translation="ISMIFQEPMTSLNPVFTIGNQISEAITLHQKIDEAEAKKRTVEM
                     LHLVGIPEPERRYNQYPHEMSGGMRQRVMIAMALSSNPDLLISDEATTALDVTIQAQI
                     LDLMRGLQKKLGMAILIITHNLAVVAEMAETIAIAYAGKIVENASAREIFKSPRHPYT
                     YGLLQSIPKFTEKRTNEPLPAIEGMVPSPYHMPKGCRFNPRCPFATEKCREEEPEILE
                     LSPGHLVRCFHPIES"
     gene            722..1696
                     /locus_tag="VN478_04655"
     CDS             722..1696
                     /locus_tag="VN478_04655"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HWQ21994.1"
                     /translation="MATRLVEVQKLKKWFPIKGGVLSTTIASVKAVDEVTFFINQGET
                     LSLVGESGSGKTTAGRTMLKLLQPTGGKIIYKGKDITNLSPNQMRPLRRNMQIMFQDP
                     YGSLNPRMPVGDIIQEPLDVHAVGTRQERRQAVATMMETVGLRPEYEKRYPHEFSGGQ
                     RQRIGVARALVINPELLVLDEPISALDVSIQSQIINLLQELQQRLNLTYLFIAHDLAV
                     VRHMSDRVAVMYLGKIVETAENDELFDHPLHPYTQALLSAVPVPDPEVKRERIILQGD
                     VPSPVNPPSGCHFRTRCPYAMPICKEQEPLAIEVAPGHTVSCHLLTKK"
     gene            1780..2025
                     /locus_tag="VN478_04660"
     CDS             1780..2025
                     /locus_tag="VN478_04660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00251.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HWQ21995.1"
                     /translation="MQISVRVIPGSSRSDVEPGDPWRVHVHARPAEGKANEELLQVLS
                     HHFGVARSAVRIVRGQASRNKTVEIGLEGIAMAEEIR"
     gene            2022..2483
                     /gene="lspA"
                     /locus_tag="VN478_04665"
     CDS             2022..2483
                     /gene="lspA"
                     /locus_tag="VN478_04665"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HWQ21996.1"
                     /translation="MSRSSTLLRWSVIVIAMILADQGTKWMARRYLELRSFTVGPVGL
                     VLTVNKGVAFGLFGGQQWVVPLNAVLGIGICVAAVVALRRGDLGLARGFLVVFGGFAG
                     NFIDRVMTGQVTDFLWVRGWSVFNVADCLVTGGAILCGLALVFPDRWGFHA"
BASE COUNT          559 a          740 c          786 g          563 t
ORIGIN      
        1 atctccatga tcttccagga acccatgacg tcgctcaatc ctgtgttcac aataggcaac
       61 cagatcagcg aagccatcac actgcaccag aagatcgacg aggccgaggc caagaagcgt
      121 accgtcgaga tgcttcatct cgttggcatt cctgagcccg agcgacggta caaccagtac
      181 cctcatgaga tgagcggtgg tatgcgtcag agggtcatga tcgccatggc gctttcgtcg
      241 aatcctgacc tcctgatctc ggatgaagcc acgacggctt tggatgtcac gattcaggca
      301 cagatcctgg acctgatgcg cggacttcag aagaagctgg gcatggcaat cctcattatc
      361 acgcacaacc tcgccgtcgt cgccgagatg gcggaaacca tcgccatcgc gtacgcaggc
      421 aagattgtcg aaaacgcgag tgccagggag attttcaagt ctccgcgcca tccctacacc
      481 tacggtttgt tgcagtctat cccgaaattc accgagaaga ggacgaacga accgctgcct
      541 gccatcgaag gcatggtccc ctcgccatac cacatgccca agggctgccg tttcaacccg
      601 cgttgcccgt ttgctacgga gaagtgcaga gaagaagaac cggagatcct tgaactgagc
      661 cccgggcatc tggttcgctg cttccatcct atcgaatcat aggcagagag gagaaatggc
      721 catggcaacc agactcgtag aagttcagaa actgaagaag tggttcccta tcaagggagg
      781 agtcctctcg acgaccatcg cctcagtgaa ggccgtagac gaagtgactt tcttcatcaa
      841 ccagggtgag acgctcagct tggtggggga gtccggatcg ggcaagacga ctgccggacg
      901 taccatgctc aagttgttgc agcccaccgg cggaaagatt atctataagg gcaaggacat
      961 cacgaatctc agcccaaacc agatgcggcc gctccggcgg aacatgcaga tcatgtttca
     1021 ggatccgtat ggttcgctga atccacggat gcctgtgggc gacatcatcc aggaaccgct
     1081 ggacgtgcac gctgtcggta ctcgccagga gcgcaggcag gctgtcgcca ctatgatgga
     1141 gactgtgggc cttcgcccag agtacgagaa gcggtatcct cacgagtttt cgggcggtca
     1201 gcgtcagcgc attggagttg ctcgagcgct tgtcatcaac ccggaactac tggtcctgga
     1261 tgagcccatc tcagccttgg acgtttcgat ccagtcgcag atcatcaact tgctgcaaga
     1321 gctccagcag agactgaact tgacctatct tttcattgcg cacgaccttg ccgtcgtacg
     1381 ccacatgtct gatcgcgtcg cggtcatgta ccttggcaag atcgtggaga ctgcggaaaa
     1441 cgatgagctg ttcgatcatc cccttcatcc ctacacgcag gctcttctgt cggcagtgcc
     1501 cgttcctgat cctgaagtga agcgcgaacg catcatcctt caaggggacg ttccctcgcc
     1561 cgtgaaccct ccgtcgggct gccatttccg cacacggtgc ccgtacgcca tgcctatctg
     1621 caaggagcag gagcctctcg caattgaggt tgctccaggg catacagtgt cctgccactt
     1681 gcttacgaag aagtaagagc cgcagacgcg cagatgtgct gggctgcctg tccctggctg
     1741 tggggatagg cggcccattt gtgttcaaca ggagacgtca tgcagatcag cgtacgcgtt
     1801 ataccgggca gcagcagatc agacgttgag ccgggagatc cctggcgagt gcatgttcat
     1861 gcccggcctg cagagggaaa ggcaaacgag gagctcttgc aggtcctcag ccatcacttc
     1921 ggtgtagccc gttctgccgt tcgtatcgtg cgagggcagg cctcaagaaa caagaccgtg
     1981 gaaatcggcc tggaaggtat cgcaatggca gaggaaattc gatgagcagg tcaagcactc
     2041 ttctccgctg gtcggtcatt gtcatcgcca tgatcctggc agaccagggt accaagtgga
     2101 tggcgcgtcg gtacctggag cttcgttcct tcacagtggg accggtgggt ctcgttctca
     2161 ctgtgaacaa gggagtcgcg tttggcctct ttgggggtca gcagtgggtc gtgccgctga
     2221 acgcagtcct tggcattggc atctgcgtcg cagcagttgt ggcgttgcgg cgtggggacc
     2281 tgggattggc ccgtggcttt ctggttgtct ttggagggtt cgcggggaac ttcatagacc
     2341 gggtcatgac tgggcaggtc accgacttcc tctgggtacg cggctggtcg gtgttcaacg
     2401 tggctgattg ccttgtaacc ggcggcgcaa tcctgtgtgg attggccctc gtgtttcccg
     2461 atcggtgggg ctttcatgcg tgagttgaga tcagggtccg tcaccgtacc agtcctgtac
     2521 ttggacgacg acattatcgc tgtcaacaag ccggcaggac tgccggtctt cactgcacca
     2581 gggaagacgg ccggaacgct taccgaggtg gcattggcga gtggggtctc gctatacgaa
     2641 ggagaggg
//
LOCUS       DATMZD010000225         3250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_75960,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000225 DATMZD010000000
VERSION     DATMZD010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75960"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1318)
                     /gene="mnmE"
                     /locus_tag="VN478_04670"
     CDS             complement(<1..1318)
                     /gene="mnmE"
                     /locus_tag="VN478_04670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HWQ21997.1"
                     /translation="MPHVSTIAAVATARGSGGIGIVRLSGPDSWAIAEQLSAGQPPLR
                     DRTANLRRVCRPGTGEVLDQGIVLPMRGPHSYTGEDTVELQLHGSPLVLDSVMAACLE
                     LGAQPARPGEFTFRALLAGKLSLLQAEAVRDLVDAGSEEELCLARESLFADASRPFRT
                     VLSLITRALASVEGSVDFPDQFTREDEASALQAATTLAAEAHTEIQRLLADAQATARL
                     QRGVTVVIAGAPNVGKSSLMNALLKYPRVIVSDVPGTTRDSVAEEILLGRHRVTLVDT
                     AGIGTTPADFLDHLAGEQARQSVERADIVLIVLDAETGLQPHERELLDRVRTIPHLVA
                     MNKVDAGPVPGSLEAEVGEPVLGISATEGTELDALTQRLTRLVETTTATVAARQMVTR
                     RQAAELTAADHALGEFVDSAGTAPADVLAYLLGEAREALQRLLGDHI"
     gene            complement(1331..1801)
                     /locus_tag="VN478_04675"
     CDS             complement(1331..1801)
                     /locus_tag="VN478_04675"
                     /inference="COORDINATES: protein motif:HMM:NF013583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HWQ21998.1"
                     /translation="MRLSKHAEQLVTEFLVDFFKALDENPEIQVAYTDGDPYINVTGL
                     TGNGTFLTRDQMCLSALQHIVNVVAHKDHEGVAPGIVTVDVDGYVMRQREMLKRMAID
                     AALRVKREKQSVMLQPMSAKARRIIHVTLKDYPGITTHSEGRDPQRRVIVELSR"
     gene            complement(1813..2973)
                     /locus_tag="VN478_04680"
     CDS             complement(1813..2973)
                     /locus_tag="VN478_04680"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HWQ21999.1"
                     /translation="MIQRSSRKSTYRLLLVATLLLGILTLSGCVAAAPVTTSDPVGVG
                     LTAVARPLENVGITVTIKNNMTKFVSDVKVMVVAVDGNPGIAPFALWQTPEPSVALTA
                     PLMPGKSAQVKLILQQKTPAAIKSYPVNVVVSYKDGDGVAVTKTITNAGIVRVGSINF
                     LSAGIRWIIEQLNNIVHSYALAIILLTFLLKLVTEPLTRMQFKSTVDMQKLQPQIAKL
                     KAKYPNKDDAQKLNADTMKLYKDNNVSIFSGCLPLLVQWPIFLALFTAINNYSPFNNA
                     PFLWAPALSMASSALPLATRFIMPVIVAASTYVQSITSFIPGQDKSQTAMLTYMMPLL
                     LGYWAYGFSIALALYWALFSLFSAAQQYVVIKRLTKGMAVPEPVVVKQAKKK"
     gene            complement(2951..3229)
                     /gene="yidD"
                     /locus_tag="VN478_04685"
     CDS             complement(2951..3229)
                     /gene="yidD"
                     /locus_tag="VN478_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017434877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HWQ22000.1"
                     /translation="MKKVVTFLLRFYRRFLSPLKGPTCIYYPTCSEYALEAVERYGVL
                     TGLWLGLKRILRCHPYHRGGFDPVPDLHRDCAHASHPLEVSVDSEKLP"
BASE COUNT          592 a          927 c         1077 g          654 t
ORIGIN      
        1 cgatgtgatc gcccaggagc cgctgcagcg cttcacgcgc ttcgccgagg agatatgcaa
       61 gaacatcggc aggggccgtt cccgccgaat ccacgaactc gcctagcgcg tggtcggcgg
      121 ctgtcagttc cgcagcttgc cggcgcgtca ccatctgccg ggccgccacg gtcgccgtgg
      181 ttgtctcgac cagccgcgtg aggcgttgag taagggcgtc gagctcggtc ccttcggtgg
      241 cggagatgcc cagcactggt tcgccgactt ctgcttcgag gctcccgggg acgggtccgg
      301 cgtcgacctt gttcatggcc acgaggtgcg gtatggtgcg gacgcggtcg aggagttcgc
      361 gttcgtgtgg ctgaagtccg gtttcggcgt cgaggacgat caggacgatg tccgcgcgct
      421 cgacggattg gcgcgcctgc tcgcctgcca gatggtcgag gaaatcggca ggggtcgtgc
      481 ctatccccgc tgtgtccacg agggtcaccc ggtgcctccc caggaggatc tcctcagcta
      541 ccgagtcacg ggttgttccc gggacgtcag agacgatgac tcgcggatac ttgagaaggg
      601 cattcatcag tgaggacttg ccgacgttgg gagcccctgc gatcacaacc gtgacaccgc
      661 gctgcaatcg tgccgttgcc tgtgcgtccg ccagcagtct ctggatctcc gtgtgtgcct
      721 cagctgcgag cgttgtggct gcctgcagag cgctcgcttc gtcctcgcgc gtgaactgat
      781 cggggaagtc gactgatccc tccacggacg caagagccct tgtgatcagg ctcaggacag
      841 tgcgaaaggg tcgtgacgca tctgcaaaca gcgactctct ggcgaggcag agctcctcct
      901 cagaccctgc gtccacgagg tcgcgcacgg cttcggcctg caggagggac agctttccgg
      961 caagcagggc ccgaaatgtg aactcgccgg gacgagcggg ctgggcgccg agttccaggc
     1021 atgctgccat gacgctgtcc aggaccaggg ggctcccatg cagctgcagt tcaacagtgt
     1081 cttcgcccgt gtaggagtgc ggcccgcgca tcggcaggac gatcccctgg tccaggactt
     1141 ctccggtgcc gggtcggcag acgcgccgta gattggccgt gcggtctcga agtggcggct
     1201 ggcctgcgct gagctgctcg gcgatggccc aggagtcggg gccgctgagt cgaacaatgc
     1261 cgataccgcc gctgccacgt gcagtggcaa cagcggcaat cgtggatacg tgcggcatcc
     1321 gccgctcctt ctacctgctg agttcgacga tgacccggcg ctgcgggtca cggccttcgc
     1381 tgtgtgtcgt aatgcccggg tagtccttga gagtcacgtg gatgatgcgc cgtgccttgg
     1441 cactcatggg ctgaagcatg acagactgtt tctcgcgctt gacgcgcaga gcggcgtcga
     1501 tagccatgcg cttcagcatc tctcgctgac gcatgacata gccgtcgacg tcgacagtta
     1561 cgatgccggg ggcgacgccc tcgtggtctt tgtgtgcgac gacattgaca atgtgctgca
     1621 gagcggacag gcacatctgg tccctggtga ggaacgtgcc gtttcccgtg agtccggtga
     1681 cattgatgta tgggtcgccg tccgtatatg ctacctggat ctcggggttc tcgtcgagtg
     1741 ccttgaagaa atccacaagg aactccgtga ccagctgttc agcgtgtttg gaaagcctca
     1801 tcctcttgcc tcctacttct tctttgcctg cttcacgacg actggctcag ggacggccat
     1861 gcccttcgtg aggcgcttga tgactacata ctgctgcgcc gccgagaaca gggagaacag
     1921 tgcccagtac agcgccagcg cgatactgaa accgtaggcc cagtagccaa gcagcaacgg
     1981 catcatgtac gtcagcattg ctgtctggga cttgtcctgt cccgggatga aggaggtgat
     2041 ggactggacg taggtcgaag ccgcaacgat gaccggcatg atgaacctgg ttgccagagg
     2101 aagcgcgctg gaggccatgg agagggctgg tgcccagagg aacggtgcat tgttgaaggg
     2161 tgagtagttg ttgatggctg tgaacagggc caggaagatg ggccactgta cgaggagcgg
     2221 aagacatccg ctgaagatgc tgacgttgtt gtccttgtac agcttcatcg tgtcagcatt
     2281 cagtttctgt gcgtcgtcct tgttcgggta cttcgccttc aacttggcga tctgtggctg
     2341 cagcttctgc atgtcgaccg tagacttgaa ctgcatgcgg gtaagcggtt cagtaaccag
     2401 cttcagcagg aatgtgagca ggatgatagc cagggcatag ctatggacga tgttgttcag
     2461 ctgttcgatg atccagcgga tccccgcgga caggaagttg atgctgccaa ccctgacgat
     2521 acccgcgttt gtgatggtct tggtgacggc tacgccgtcg ccatccttgt aggacaccac
     2581 gacgttgacg gggtacgact tgatagctgc gggcgtcttc tgctgcagga tcagcttcac
     2641 ttgggccgac ttgcctggca tgaggggtgc tgtcagcgcc acagagggct cgggggtctg
     2701 ccagagggca aagggtgcga tgcccgggtt gccgtcgacg gcgacgacca tgaccttcac
     2761 gtcgctcacg aacttggtca tgttgttctt gatagtgacg gtgatgccga cattctccag
     2821 agggcgtgct acggccgtga ggccaacgcc tacaggatcg gaggtcgtga cgggagcggc
     2881 cgcaacacag ccggacagcg tcagtatgcc gaggagcaag gtagcgacga gcagcaatct
     2941 gtacgtgctc ttacgggagc ttctctgaat caacggaaac ctccaggggg tgagatgcat
     3001 gcgcgcagtc acggtgcagg tcgggcacgg gatcgaaccc tccccgatga tatggatgac
     3061 accgaagaat acgcttgagg ccgagccaca gcccggtcag gacgccgtac cgctcgacag
     3121 cctcgagcgc gtactccgaa cacgtgggat agtagatgca ggtcgggccc ttgagcggag
     3181 agaggaatcg acggtagaag cgcagcagga acgtaacgac cttcttcatt gctggcggta
     3241 ctgggcagtc
//
LOCUS       DATMZD010000226         2838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_107001,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000226 DATMZD010000000
VERSION     DATMZD010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107001"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="VN478_04690"
     CDS             <1..497
                     /locus_tag="VN478_04690"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HWQ22001.1"
                     /translation="LLLLARLDREQPAPLAPVQLLNVWTDLHNAHADALSAKSVTLQT
                     PSEALAVTANRDLLTVALDNLIDNAVRYAPEGTPVIITAQQTKDRVTIAVIDQGPGMP
                     PDALPHIWDRFSRVDPSRSRESGGNGLGLAIVHKAVEAMHGQVAVTSEVGKGSTFSII
                     LPTS"
     gene            complement(553..1002)
                     /locus_tag="VN478_04695"
     CDS             complement(553..1002)
                     /locus_tag="VN478_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGGC domain-containing protein"
                     /protein_id="HWQ22002.1"
                     /translation="MSEEIVRIGMIMCNRYHTCAAGKCLRALRNREGAFSIYAGRQVE
                     LVGCTTCGGCPGGNIEYAPAEMKKNGVTVIHLATGMLVGYPPCARIDYFRRFIREQYD
                     IPVVVGTHPIPQKYFKIHDALGSWKSPMWQELIQPTLATEEIRLAYD"
     gene            complement(1331..2194)
                     /locus_tag="VN478_04700"
     CDS             complement(1331..2194)
                     /locus_tag="VN478_04700"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HWQ22003.1"
                     /translation="MALADNEQSMTSQVNHVRHVLAVASGKGGVGKSLVSAMAAVGLA
                     GRGLKVGIMDADITGPSIPRLFGMTARPESSMVGIIPPETATGIKIISMNLFLDNSGQ
                     PVIWRGPLLTSAIKQFWTDVAWGDLDVLIVDLPPGTADVPLTAFQSLPIDGILMVTTP
                     QELASMIVEKAAGLAKQMHKPVLGIVENMAMVTCPHCGEVFEVFGPSHADELADHIGA
                     AVLARLPIDPTVTTLADKGHVEDVSMAAFAPVTEALAEYVTTPSTVFAAPNSAPDPAT
                     TPSCSADLRDC"
     gene            complement(2181..>2838)
                     /locus_tag="VN478_04705"
     CDS             complement(2181..>2838)
                     /locus_tag="VN478_04705"
                     /inference="COORDINATES: protein motif:HMM:NF014620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HWQ22004.1"
                     /translation="RILGADEMEALRLSDFLELDQTEAARRMGVSQSTFHRILAGARR
                     KVVASLVSGCPLTVARAWDADVNTMAIHRQQEKTGGTSMVTRIALPTDDGTTLCQHFG
                     RAHYYKIVEMTDGKVTATEMRDKFAHVCGDPRMDSGLTHEEVHGRLAEAAHGCDTVVA
                     GGMGQGAMDALTAAGMKVIQTDMVNLDDIVAAYTAGTLASVVGQMHCCHHEGEHHGAC
                     "
BASE COUNT          536 a          904 c          829 g          569 t
ORIGIN      
        1 ccctgctcct gctggcacgc ctcgaccgcg aacagcctgc cccacttgcg ccggtgcagc
       61 tgctgaacgt ctggaccgac ctgcacaacg cgcatgccga tgcattgagt gcgaagtcgg
      121 tgacactgca gacaccctcc gaggcactgg ccgtcactgc caaccgggac ctcctgaccg
      181 tcgcgctcga taacctgatc gacaacgctg tccgctatgc tcccgaggga acacccgtca
      241 tcatcacggc tcagcagacc aaagaccgag tgacgatcgc cgtcatcgac cagggacccg
      301 gcatgccgcc ggacgcgctg ccacacatct gggaccgctt cagtcgtgtg gacccaagcc
      361 gaagccggga atccgggggg aacggcctgg gcctggccat cgtccacaag gcagtggaag
      421 ccatgcacgg ccaagtcgcc gttacgagcg aggtcggcaa gggaagcacg ttctccatca
      481 tcctgccgac ttcctagctc tcatcaattg tgaagaccat tgtgcctgac cccatggtct
      541 tcacaatgca gtctagtcgt atgccagtct gatctcctcg gtagcgagcg tgggctggat
      601 gagctcctgc cacatgggag acttccagga gcccagcgcg tcatggatct tgaagtactt
      661 ctgggggata gggtgcgttc cgacgacgac agggatgtca tattgctccc ggatgaagcg
      721 tcggaaatag tcgatgcgcg cgcatggtgg atagccgacg agcatgcccg tggcaaggtg
      781 gatgacggtc accccgttct tcttcatctc ggcaggcgcg tactcgatgt tgcctcccgg
      841 gcacccgccg cacgtggtgc atccgacaag ctccacctgt ctccccgcgt agatgctgaa
      901 tgccccctcg cggttcctca aggcgcgcag gcacttgccc gctgcacagg tgtggtacct
      961 gttgcacatg atcatcccga tacgtacgat ctcctcactc attggacctc ctcatcacag
     1021 aacggactta caacgacaag tctagcagag ttcagtcagg ctgagcagag caccagaact
     1081 cttctgccca ctgggtggct ggatacgtgt cgtacttctg ccacatggtg cccgcgtgac
     1141 gccggccact ggcgcaccgg ttcaccagca atgggaccca gacggtagac actccttcgt
     1201 tctcacctgc catgacggat agtgcgttat gcatcatgcg tcatcatctc tggtactctg
     1261 tatgcacaag ccctgtcgca ggattctgca ccagggcttg tggcacttca tgaaagctgg
     1321 attgcgtccg tcagcagtct ctcaagtccg ccgaacatga aggggtggtg gccgggtcag
     1381 gtgcggaatt cggagcagcg aagactgtag acggagtggt aacatactcc gccagagcct
     1441 cggtgactgg ggcgaaagcc gccatgctca cgtcctccac gtgtcccttg tcggcaagag
     1501 tagtcacggt ggggtcgatc gggagccgcg cgagcacggc ggctccgatg tggtcggcca
     1561 gctcgtcggc atggctgggt ccaaatacct caaacacctc gccacagtga ggacatgtga
     1621 ccatcgccat gttctcgacg atacccagga ccggcttatg catctgcttt gccaggccgg
     1681 cggccttctc gacgatcatg ctggcgagtt cctgtggtgt cgtgaccatc aggatcccat
     1741 cgatcggcag cgactggaac gccgtcaggg ggacgtcggc cgttccgggc ggcaggtcga
     1801 cgatgaggac gtcgaggtct ccccacgcca catcagtcca gaactgcttg atggcagacg
     1861 tgagaagtgg gccgcgccag atcaccggct ggccggagtt atccagaaac aggttcatgg
     1921 agatgatctt gattccagtc gccgtttcgg gcgggatgat cccgaccatc gacgactcag
     1981 gccgcgcagt catgccgaac aggcgcggga tggatgggcc ggtgatgtcg gcgtccatga
     2041 tgcccacctt cagtccacga cccgccagcc cgaccgccgc catggcagag acgagggact
     2101 tgcccacgcc acccttgcca ctggcgacgg ccagcacgtg ccgcacgtga ttgacctgcg
     2161 aggtcatgct ctgttcgttg tcagcaagcg ccatggtgtt ctccttcgtg gtggcagcag
     2221 tgcatctgtc cgaccacact cgcgagtgtc ccggccgtgt aggccgcaac gatgtcgtcg
     2281 agattgacca tgtcggtctg gatgaccttc atgcccgcgg ccgtcagcgc atccatcgcg
     2341 ccctgtccca tgccaccggc aacaacggtg tcacagccat gtgcagcctc ggccaggcgt
     2401 ccatgcactt cctcgtgcgt gagcccactg tccattcttg gatcaccgca gacgtgagcg
     2461 aacttgtccc gcatctcggt ggcagtcacc ttgccgtcgg tcatctcaac gattttgtag
     2521 taatgagctc gtccaaagtg ctgacacagc gttgtgccat cgtcggtcgg tagtgcgatt
     2581 cgtgtaacca tgctcgtacc tcctgttttc tcctgctgtc tgtggattgc catggtgttc
     2641 acatctgcat cccatgccct tgcgactgtc agtggacatc cactcacaag agatgccacg
     2701 accttgcggc gggcacctgc aagaatgcga tggaacgtgc tctgggagac gcccatgcgg
     2761 cgtgccgctt ccgtctgatc cagttccaga aagtcgctga ggcgcaatgc ctccatctcg
     2821 tcggccccga gtatacgc
//
LOCUS       DATMZD010000227         2388 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_126901,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000227 DATMZD010000000
VERSION     DATMZD010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126901"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1172)
                     /locus_tag="VN478_04710"
     CDS             complement(<1..1172)
                     /locus_tag="VN478_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22005.1"
                     /translation="MKKFLSLFVAVAMMFSLFAGFKAPAAKAAGANGIAIIDATGTKL
                     ISSDDDNTQDYYSTNPTLTTEGTLATMDDGDYVYNMGDIITGKLTLGGVTTVPTSPWQ
                     VSLMQFPNVVIDFTVVSAGVSTFTIGTANVRFDGPYFLRVHSLLGEFPDYDIFNNIVE
                     GDDVANLYIKYNVAWVLDTITPCPQLQKISGYVTRGNGQTVDVPVFVGVAYPGVTADG
                     VLASYYSIAKASSGQFAMTFLGMANPGLYRVFFMDGYYDTANSAAPGSTDVLNYSHGV
                     ITTGTNASNFVLDHDAMVYKTISTIPTAFAMKAALFTNPVYLYKNTAGQSVLISLTDA
                     FGKPVTGAYWYVENTAGTVISDNDPATAVLADVNFAGSEIDSGYYSFAINTSSYSGT"
     gene            complement(2030..>2388)
                     /locus_tag="VN478_04715"
     CDS             complement(2030..>2388)
                     /locus_tag="VN478_04715"
                     /inference="COORDINATES: protein motif:HMM:NF019450.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="copper amine oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HWQ22006.1"
                     /translation="GTTGVTLDSAPYIDKASGRTMVPMRFIGEAFGASVAWDAGSRRV
                     SVETQGTQNHQALSMVMTISSRKATVNGKSVTLDVAPAIVAGRTFIPLRVISETLGAQ
                     VSWNGTTRVVGIEYLP"
BASE COUNT          500 a          650 c          758 g          480 t
ORIGIN      
        1 gtgcccgagt agctggaggt gttgatcgcg aagctgtagt acccggaatc gatctctgag
       61 ccggcgaagt tgacgtcggc aagaacggcc gtagcagggt cgttgtccga gatgactgtc
      121 cctgccgtat tctctacata ccagtaagca ccggtgacgg gcttgccgaa cgcatcggtc
      181 agggagatga ggacactctg gccagccgta ttcttgtaga ggtacacggg gttggtgaac
      241 agggcggcct tcatcgcaaa cgccgtcggg atggtgctga tggtcttgta gaccatagca
      301 tcatgatcca ggacgaagtt cgaggcgttg gtcccagtcg tgatgacgcc atgagaatag
      361 ttcaggacat cagtcgatcc aggagcagca ctgttggccg tgtcgtagta cccgtccatg
      421 aagaagacgc ggtacaggcc ggggtttgcc atgcccagga aggtcatagc aaactggcct
      481 gaggaggcct tggcgatgct gtagtaggaa gcgagaacgc cgtcagcagt gacgcccgga
      541 tacgcgacgc ccacaaacac ggggacatcg acggtctggc cgttgccacg ggtcacgtac
      601 ccgctgatct tctgaagctg tgggcatggg gtgatggtgt ccaggaccca ggccacgttg
      661 tacttgatgt acaggttggc aacgtcgtcg ccttcgacga tgttgttgaa gatgtcatag
      721 tccgggaact cacccagaag gctgtgaacg cggaggaagt acgggccatc aaagcgcacg
      781 ttggctgtgc caatggtgaa ggtgctgacg cctgccgaga cgacggtgaa gtcgatgacg
      841 acgttgggga actgcatcag cgaaacctgc cacggggagg tcggcacggt ggtaacgccg
      901 ccaagtgtca gcttgccggt gatgatgtcg cccatgttgt acacatagtc gccatcgtcc
      961 atcgtggcca aggtgccctc ggtggtgagg gtaggattgg tcgagtaata atcctgagtg
     1021 ttgtcgtcgt cggacgaaat cagcttcgtg cctgtggcat caatgatcgc gatgccgttg
     1081 gcgcctgctg ccttcgccgc gggcgccttg aatcccgcaa agagcgagaa catcatggca
     1141 accgctacga acagagatag aaacttcttc attgtctctc ctttagagtg tgagatggat
     1201 gctgaaagaa gttgtggtcg aatgcgaccg tttggcgcac gcaggcgccg cgggagtccc
     1261 gccagtgtgg accgttcctc agaagtgccc ctcggactga acaaaattcc tcgggaggat
     1321 gttccgagtt gcctctgcca cggtgcgccc cctgcatgga gaagacatgc ccggggggac
     1381 agcagagacg ccctcgaccg atccgttccc agcccggact gagcaccaga acatcgacaa
     1441 ctgcggagac tgttcgcgtc gccgaagcag agccggtcca gtgtgggacc tggcaggagt
     1501 ccgtgttgtg tgggcaccct ggggcgccac atcctgcggt gaaccatcct tcgtctgcct
     1561 ggtgagtgga gcagtcacga tgccgaatcc tgaagcacgt ggcaggaacg aaacagcgaa
     1621 atagctgaga tacgagaacg cgggtgaagc aggtagcagg tatcgcatac ctgagtaaac
     1681 cgttgtcaga gatatcggac cgcgatacga ctattcggtc cgtgtcatgc gtggtatctt
     1741 gcccttcagt tgtcaacgcg ctggtggagc cgtttccgac ccaccgtcga ctgccctttc
     1801 ggactgccgg aagaagagaa tgcactatcg agacagactt cttccgctct catgggtgca
     1861 caatacgtgc caagtgacga ggtatggagg gtaaagcatt tccaatagag cgtttcagtg
     1921 cgctcgtggt tcgtcagacg gggaggccgg acctcacaaa tgggcggtac ggcctcggtt
     1981 aggtgtgcca tgtgtacaca ctggtgtacc ctcagggcac accggtttgt tacggcaggt
     2041 actcgatacc caccacacgg gtcgtgccat tccatgacac ttgtgcgccg agcgtctcgg
     2101 agatgacccg tagtgggatg aacgtgcgtc cagcgacgat ggccggcgcc acatcgagcg
     2161 tcacggattt gccgttcaca gtggccttcc tgctgctgat cgtcatgacc atggagagcg
     2221 cctgatggtt ctgcgtaccc tgagtctcga cggacacacg gcgggagcct gcatcccacg
     2281 ccacagaagc gccgaacgcc tcgccgatga accgcatcgg gaccatcgtt cgtccgctcg
     2341 ccttgtcgat gtacggggcc gaatccagcg tcacgccagt cgttccga
//
LOCUS       DATMZD010000228         6831 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_116440,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000228 DATMZD010000000
VERSION     DATMZD010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6831
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116440"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /locus_tag="VN478_04720"
     CDS             complement(<1..811)
                     /locus_tag="VN478_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HWQ22007.1"
                     /translation="MKAREDHTYIAIDLKSFYASVECVERGLDPLTTNLVVADASRTE
                     KTICLSVSPALKSYGIPGRARLFEVIQKVKEINAGRQRTAPGRAFTGSSCSDLELKAS
                     PNLSLDYIIAPPHMARYMEVSTRIYSIYLQYVAPEDIHVYSVDEVFMDVTNYLSTYKL
                     AARELTMKIIHNVLGITGITATAGIGTNMYLCKIAMDIEAKHMAPDKDGVRVAELDEM
                     SYRRSLWNHRPLTDFWRVGRGYAKKLEENGLCTMGDVARCSIGKPDDHYNEG"
     gene            complement(899..1546)
                     /locus_tag="VN478_04725"
     CDS             complement(899..1546)
                     /locus_tag="VN478_04725"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWQ22008.1"
                     /translation="MNAFQYLARNWPEVGAKVVQHLGMVGIAVAISLVVGLALGIVVS
                     RPGLQKVGRAIISVTGVAQAIPSVAVIALMFVYTGIGPRTAVIALSIYGVVPILFNVS
                     SAMLLVPSDMREAAGGMGMTPLQVLTRVELPLASQSIMAGLRTTVTIDVSTATVASVV
                     GAGGLGDIIFVGLSVVRPDMIVAGTLLVASIAILSDALLALLQKSVVSRGLSTPS"
     gene            complement(1543..2622)
                     /locus_tag="VN478_04730"
     CDS             complement(1543..2622)
                     /locus_tag="VN478_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWQ22009.1"
                     /translation="MIEVDSLTKRYGDKTSVDGLSLTVPDNAITILLGPSGCGKTTTL
                     KMINRLIEPTSGDIRIDGRSVYSMDPIELRRSIGYVIQEIGLFPHYTVEGNIGVVPRL
                     LGWDRSRIRRRAQELLELVNLDPSVYLGRYPRELSGGERQRVGVARALAADPHILLMD
                     EPFGAIDPINRRSLQDFFLRLQSDLKRTVVFVTHDIGEAVKLGDYIAVMRDGRLVQMD
                     ATRALLEHPADTFVEDLLGATRSVEQLLLYRAAHYVDTAFTVMTTEEMRRSDLQAEAP
                     LIIVKDGDVVSGYVEAGQFVTCEGPEPAVCRPDIISGNATLLEAVSTLLESGHTRAVV
                     QDPDGTILGTLSVPMVVDIMAKVAR"
     gene            complement(2619..3281)
                     /locus_tag="VN478_04735"
     CDS             complement(2619..3281)
                     /locus_tag="VN478_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014451656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWQ22010.1"
                     /translation="MIATFIAGYGAEMLRRTFQHMSMVGLALVLAASIGVSLGVAISG
                     HERAARWVMGAASVLFTVPSLALFALLVVALAPIRQGTGRVPAVTALTLYSILPIVRN
                     TWVALRQVDSSTVQAAQGMGMTPRQVMLRVKLPLALPLIMAGVRNAAVLGVGMATIAT
                     LIGGGGLGYFIFEGIARANRAMVIAGTLAITVLGLAVNGGLLMAEEALTPGRRRRAEH
                     TR"
     gene            3674..4531
                     /locus_tag="VN478_04740"
     CDS             3674..4531
                     /locus_tag="VN478_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HWQ22011.1"
                     /translation="MNTIRKREELGHNVIRYVVDAPLVATHRKPGQFVIIRVTDHGER
                     IPLTIADANVEMGTISLIVQAVGKTTREMATLRPGNVIHDIVGPLGRPTHIQRFGTVL
                     CVGGGIGTAPLFPIAKGMKEARNRVITVLGARTHDLLILEDDMRNVSDELIVTTDDGS
                     YGAKGLVTDAIRSLVGGGTHIDQAVVIGPPVMMKFTSLLTKELGIPTMASLNPIMIDG
                     TGMCGGCRVTVGGEIKFACVDGPEFDAHEVDWDSMIRRLGTYRTPEQAAADRYDHECH
                     VGLNAGKPL"
     gene            4528..5955
                     /gene="gltA"
                     /locus_tag="VN478_04745"
     CDS             4528..5955
                     /gene="gltA"
                     /locus_tag="VN478_04745"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HWQ22012.1"
                     /translation="MNAAERMKLSPVIMPEQDPAERVHNVREVPCGFTPAMAGQEALR
                     CLHCANQPCVAGCPVGVRIPDFIQAIESGDYLSGIGIIKQTNLLPAVCGRVCPQEEQC
                     QKDCTVGKAHRDVGQAVQIGKLERFLADWERENHAAVIPEIPPATGKRVAVIGSGPAG
                     LTVAGDLIKLGHAVTVFEALHLPGGVLGYGIPEFRLPKEIVYQEVAYLTRLGVDFQYN
                     TVIGKSLTVDEVRAAYDAVFIGSGAGLPNFLGIPGESLLGVYSANEYLTRANLMHAYD
                     ENSDTPILHGKSVAVLGGGNVAMDSARTALRLGAIRVMVIYRRSEEEMPARKEEYHHA
                     VQEGVEFHFLQNPLEILGNREGWVTGLTVQQMQLGEPDASGRRRPLPIAGRTSNVPAD
                     IVVVAIGNNPNPLIPAALPSLQINKHGGIVADEDTMETSVPGVFAGGDIVLGAATVIL
                     AMGQGRKAARAIHTWLTRTADAAGS"
     gene            6097..6738
                     /locus_tag="VN478_04750"
     CDS             6097..6738
                     /locus_tag="VN478_04750"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HWQ22013.1"
                     /translation="MDKLKRFMKENWWRYLMNLVLAGGVYWSSTWYAMTNHATANVHI
                     LQTAFDRWVPVVPQFIAPYNWLEPVLYFCLLFFFVFHPKVFTAFGVAFIAIQAISNGV
                     YVVFQTQVPRPTNLVPGSSRYVDALLAKYASDNPYNCFPSLHCGLSALAASFWWVKRR
                     YRPVAWLMTAFAVVTVIATQVLKQHVIVDAVGSLLAIGLYLLAYRIFNLKTEA"
BASE COUNT         1392 a         2148 c         1997 g         1294 t
ORIGIN      
        1 gtccttcgtt gtaatggtcg tccggttttc cgatcgagca cctcgccacg tcgcccatcg
       61 tgcagaggcc gttttcctcc agctttttgg catagcctct gccaacgcgc cagaaatccg
      121 taagcggacg atggttccac aaagaacggc gataactcat ttcgtcgagt tcagcgacac
      181 gcacaccatc cttgtcggga gccatgtgtt tggcctctat atccatagcg attttgcaga
      241 gatacatgtt ggtgccaatg cccgctgttg ccgtgatgcc tgtgatgcca agaacattgt
      301 ggatgatctt catggtgagt tcgcgagcag caagcttata ggtactcagg tagttcgtta
      361 cgtccatgaa gacttcgtcg acggaataga catggatgtc ttccggagca acatactgca
      421 gatagatgct gtagatccgg gtgctgacct ccatgtaccg tgccatgtgt ggcggtgcga
      481 taatgtagtc aagtgacagg ttcggggatg ctttcaattc caggtcactg catgaggagc
      541 cggtaaaggc tctgccggga gcagttcgct gccgccctgc gttgatctct ttgaccttct
      601 ggatgacctc aaacagcctg gctcgccccg gaataccata ggatttgagt gctggtgaca
      661 cagagaggca gatagtcttc tcggtacggc ttgcatcggc cacgacaaga ttcgtggtaa
      721 gcgggtcaag cccgcgctca acacactcga ccgaggcgta gaaggacttc aggtcgattg
      781 cgatatatgt gtgatcctcc cgagccttca tctgcgcctc cgatgtcctg gtaacacccg
      841 gccgatgccg gatgtggtct ctccattcgc cttcatgcgt gattcatgct cgtttgtcct
      901 aagacggtgt ggacagtccg cgcgagacca cggatttctg aagcagggcg agcaacgcat
      961 cagacaagat ggcaatggat gctacgagaa gcgtcccggc gacgatcatg tcggggcgta
     1021 cgacgctgag accaacgaag atgatgtcac ccaggccccc cgccccgacg accgacgcga
     1081 cagtggcggt cgacacgtcg atcgtcaccg tcgtccgcag accggccatg atcgactgcg
     1141 acgcaagcgg cagttcgacc cgcgtgagta cctggagagg ggtcatcccc atcccaccgg
     1201 ccgcttcgcg catgtccgac ggtacaagca gcatggctga agagacgttg aacaggatgg
     1261 ggaccacgcc gtagatggac agcgcaatga cggccgtgcg ggggccgatg cctgtgtaca
     1321 cgaacatgag ggcgatgacg gcgacgctcg ggatagcctg ggcgaccccc gtgacactga
     1381 tgatcgcccg tccaaccttc tgcagccccg gccggctgac gacgatgcca agcgcgaggc
     1441 ccacgaccag tgagatggca accgcgatcc caaccatacc gagatgctga acaaccttgg
     1501 caccgacctc cggccagttc ctggcaaggt actggaaagc gttcaccggg ccaccttcgc
     1561 catgatgtcg acaaccatcg ggacactcaa cgtccccagg atggtgccgt cagggtcctg
     1621 caccaccgct cgtgtgtgtc ctgattcgag cagcgtcgag acggcctcga gcagcgtagc
     1681 attgccgctt atgatgtccg gccggcaaac cgcaggttcg gggccctcgc acgtgacgaa
     1741 ctgccccgcc tcgacatagc ctgagacaac atctccatcc ttgacgatga tgagcggggc
     1801 ctctgcctgc agatccgaac gacgcatctc ctccgtcgtc atgaccgtga acgcagtgtc
     1861 gacgtagtgg gcggcccgat acaacaacag ctgctcgact gaacgcgtcg cacccagcag
     1921 gtcctccacg aacgtatccg ccgggtgctc gagcaaggcg cgcgtagcgt ccatctgcac
     1981 caggcgtccg tcccgcatga cggcgatata gtcacccagc ttgaccgcct cgccgatgtc
     2041 atgggtcacg aagacgaccg tgcgcttcag gtcgctctgc agtcggagaa agaagtcctg
     2101 gaggctgcgc cggttgatgg gatcgatggc cccgaacggt tcatccatga gcagaatgtg
     2161 ggggtcggct gccaaagcac gcgccacgcc cacacgctgg cgttcgccgc cagacagctc
     2221 acgcggatag cgccccaggt acaccgatgg gtccagatta acaagctcca gcaactcctg
     2281 tgcccgacgc cggatccggg acctgtccca tcccagcagg cgaggaacca cgcctatgtt
     2341 tccctccacc gtatagtgag gaaacaggcc gatctcctga atgacgtatc cgatgctgcg
     2401 cctcagctcg atgggatcca tgctgtagac actgcgcccg tctatgcgga tatctccgct
     2461 cgtcggctcg atcagcctgt tgatcatctt gagcgtcgta gtcttgccac accccgaagg
     2521 gcccagcaat atggtgatgg cattgtccgg aacggtcagt gacaggccgt cgacagacgt
     2581 cttgtcgcca tatcgtttcg tcagcgaatc cacctcgatc atcgtgtgtg ctccgccctc
     2641 cggcgccttc ccggcgtcag agcttcctct gccatcagca gaccaccgtt gacggccagt
     2701 ccgagcacag ttatggcaag cgttccagcg atgaccatgg cccggttggc ccgggcaatg
     2761 ccttcaaaga tgaagtagcc gagtccgccc cctccgatga gcgtcgcgat cgtggccatt
     2821 cctacgccca ggacggctgc gttgcgcacg cctgccatga tgagtggcag agccagaggc
     2881 aacttcacgc gaagcattac ctgccggggt gtcatgccca tcccctgcgc ggcctggacc
     2941 gtcgacgaat ccacctgacg cagcgccacc cacgtgttcc gcacgatcgg aaggatagag
     3001 tacaaggtca gtgctgtgac ggcgggcacg cgtcccgtcc cctgccggat aggcgccagt
     3061 gcgaccacaa gcagggcaaa cagggcgaga gacggcactg tgaacaggac cgacgctgct
     3121 cccatgaccc agcgggcggc cctctcatgc ccggaaatgg cgacgccgag cgacacgccg
     3181 atagaggctg ccaggacaag ggcaagcccg accatcgaca tgtgctggaa cgtgcgccga
     3241 agcatctctg ccccatatcc cgcaatgaac gtcgcaatca tcgtctctcc tctctgaact
     3301 aggttatacg acaattgcgg cagaactgca atcctgtaag aaaccatgct cttgtgtcgg
     3361 aacagcagac acaaaccagt ctccatatgg gttccagggg cgctgaacct tgacagactt
     3421 tgatggagct catcacatgg cctgcctgca tccgttgtct cgcccaatga gatggtgcac
     3481 ggccacaaaa gggaaggctc cggctctctc accgcccccc tgtttcggag agcgacggct
     3541 ttggtcgtcc gctctccatt ggctcgcaac aggctctcga ctgcgtcgcc ttcgcgacgg
     3601 gcagtctcat gcgtccgtcc gttttggcat atactgtgat gaagtcatac gtcaggaaaa
     3661 ccgagggacc cgaatgaata cgatccgcaa acgggaagag ctgggacaca acgtcatcag
     3721 gtacgtcgtc gacgcgccac tcgttgcgac gcacaggaaa cccgggcaat tcgtcatcat
     3781 ccgggtgaca gatcatggcg agaggattcc gctgacgatc gccgacgcga acgtcgagat
     3841 ggggaccatt tctctgatcg tccaggctgt cggcaagacc acacgcgaga tggcgacgct
     3901 gagacccggg aatgtgatcc acgatatcgt gggacccctg ggtcgaccaa cacacatcca
     3961 gcggttcggg accgtgctgt gcgtgggtgg cggcatcggg acggctccac tgttccctat
     4021 cgccaagggc atgaaggaag cccgcaaccg tgtcatcaca gtcctgggcg ccaggacgca
     4081 cgacctgctc attcttgaag acgacatgcg caatgtcagc gacgaactca tcgtcaccac
     4141 agacgatggc tcttacggcg ccaaggggct cgtcacggac gcgatccgtt ctctcgtggg
     4201 aggagggaca cacatcgacc aggctgtcgt catcggtccc ccggtcatga tgaagttcac
     4261 gtctctgctc acaaaggaac ttggtatccc tacgatggca tcgctgaatc ctatcatgat
     4321 cgacggaacc ggcatgtgtg gcgggtgccg cgtcacagtg ggcggcgaga tcaagttcgc
     4381 ctgcgtcgac ggtccagagt tcgacgctca tgaggtcgac tgggacagca tgatcaggcg
     4441 tctcgggaca tatcggacgc cggagcaggc agctgccgac cgctacgatc atgagtgcca
     4501 cgtcggtctg aacgcaggga agccactatg aacgctgctg aacgaatgaa gctgtcaccc
     4561 gtcatcatgc ccgagcagga ccccgctgag cgcgtacaca acgtccgcga ggtcccctgc
     4621 gggttcaccc ctgcaatggc tgggcaggaa gcactgcgct gcctgcactg cgccaaccag
     4681 ccctgtgtgg ctggatgccc tgtcggcgtg cgcattcccg acttcatcca ggctatcgag
     4741 tccggcgact atctctccgg gatagggatc atcaagcaga cgaacctgct gccggccgtc
     4801 tgcggccgcg tgtgcccgca ggaagagcaa tgccagaagg attgcacggt aggcaaggcc
     4861 cacagggacg tcgggcaggc cgtgcagatc ggcaaactcg aacgattcct tgccgattgg
     4921 gagcgggaga accacgctgc ggtcatcccc gagatcccgc cggcgactgg aaagcgagtg
     4981 gccgtcatcg gcagtggccc cgcaggcctc accgtcgctg gtgacctcat caagctgggc
     5041 catgccgtca ccgtgttcga agcgctgcac ctgccgggag gcgtcctggg ctacgggatt
     5101 cccgagtttc gtctgccaaa ggagatcgtc tatcaggaag ttgcctacct gaccaggctt
     5161 ggtgtcgact tccagtacaa cacggtcatt ggcaagtccc tcaccgtcga cgaggtgcgc
     5221 gcggcttatg atgcagtctt catagggagt ggcgcagggc ttccgaattt cctgggcatc
     5281 cccggcgaga gcctgctcgg cgtctattct gccaacgagt acctgacacg cgccaacctc
     5341 atgcacgcgt acgacgagaa ctccgacacg cctatcctgc atggcaagtc agtggccgtc
     5401 ctgggtggcg gcaacgtcgc catggattcg gcccggacgg cgctgcggct gggagccatc
     5461 agggtcatgg tgatctaccg gcgttccgaa gaggaaatgc ctgctcgcaa ggaggaatac
     5521 caccatgccg tgcaggaggg agtcgagttc cactttctcc agaaccccct ggagatcctc
     5581 gggaaccgtg agggatgggt cacaggcctg actgtacagc agatgcagct cggcgaaccc
     5641 gatgcttcag gtcgcagaag accccttccg atcgcgggtc ggaccagcaa tgtccccgcg
     5701 gacatcgtcg tcgtcgcgat cggcaacaat ccaaatcctc tcattcccgc agctctcccg
     5761 agtctgcaga tcaacaagca cgggggcatt gtcgccgacg aagacacaat ggagacgtcc
     5821 gtccctggag tcttcgcagg gggagacatc gtactcggag ctgcgacggt catcctggcc
     5881 atgggccagg gccgcaaagc tgcgcgtgcc atccacacct ggctcacgag gacagctgac
     5941 gcagctggga gctagggccg ggaacacaga agtcacccgt gggccggagc acctctccgg
     6001 ccctcttgcc gcccttgtgc tgtgagcgtc cagggctatc atgcctcatg tacccgcacg
     6061 cagtatcagc gagctgcagg cggagaggag gaaacgatgg acaagctgaa gcgattcatg
     6121 aaggagaact ggtggagata cctgatgaat ctcgtcctcg ccggcggcgt ctactggtcg
     6181 tcgacctggt acgccatgac caaccacgcg acagccaacg tgcacattct ccagacagcc
     6241 tttgatcggt gggtcccagt cgtcccccag ttcatcgccc cctacaactg gctggaaccc
     6301 gttctctact tctgcctcct gttcttcttc gtcttccatc caaaggtctt cacggcattc
     6361 ggcgtggcgt tcatcgccat ccaggccatc agcaacggcg tctacgtcgt gtttcagact
     6421 caggtgccac gccccacgaa cctggtgccg ggcagcagca ggtatgtcga tgccctgctg
     6481 gccaagtacg caagcgacaa cccctacaac tgcttcccgt cgcttcattg cggcctgtct
     6541 gctcttgccg ccagcttctg gtgggtcaag cgacgctacc gtcccgtcgc ctggctgatg
     6601 acggcgtttg ccgtggtcac ggtcatcgcc acgcaggtcc tcaaacagca cgtcattgtg
     6661 gacgcggtcg gcagtctgct cgccataggg ctgtacctgc tcgcctatcg catcttcaac
     6721 ctgaagaccg aagcctgatc ctcagatttc ctccgacaat atggtccggc gacatccacg
     6781 tcgccggact ttcagtatcc tcaggagact cctacgtcgg atagcctgaa c
//
LOCUS       DATMZD010000229         3851 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_97239,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000229 DATMZD010000000
VERSION     DATMZD010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97239"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2544
                     /locus_tag="VN478_04755"
     CDS             <1..2544
                     /locus_tag="VN478_04755"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HWQ22014.1"
                     /translation="AGGGGFLGFKGAPIVYQSSDEIRNLMIQYVKDAGTIDPTVDDNW
                     HVIPDYLSSQYRAAIDTLNRQSALSEFPTAAFAPESIANRADLAAILTGSYTSVGSRD
                     HGSSFLDVASDSWYARAVGTVEALKMMNGTSAKTFTPDAPVTTEQAVTALMRASGYGY
                     EASRYDLCAQPLDKVPNLFRNTDGTWNWEALAADTKKNGGATINMRSGQSLGGVEMYA
                     IALPVAPGMVVNGYPTGADLKAFTMKNAKWLLDRTGYALGTWYYKGKTYIDVSTTIPW
                     RDYAITMGKEYDQISIFDLANFEEIPTGGTSGKSGKKPNMNLIISDWALPYVAFAESK
                     GFITAEQATAPKHQLTRGELADMVVKMRFPTVVILHTNDFHGNLVPVIDANKNSVAGA
                     AREATIINTMRSTFGADHVLLVDAGDAIQGATVANLFQGKSVTDAYNAMGYNVATLGN
                     HEWDYGQEVLKERIADAKFDYVNSNLTGTNLGWKSSVIKNIAGINIGLFGLMTSDYPI
                     IVTPSSQTNVTVGDPIASAKTEVADLKAQGAQYVVALSHCGYENGGAPLDPAIAAGAP
                     GINLIIGGHSHTVLKTAVMVGKTMITQTGTAGAYVGKEVIDFTTYQGKVTGQSVSYEL
                     VPTVTSVAEDPAVKAIVDGYNNQLTAKLSVVIGKALVALNGERADVRTKETNLGNYVT
                     DWMRISTGADIAFMGGGSIRVTVPAGDVTVGNVYAVLPFDNFLEVLEITGSTLKACLE
                     NSVSAYPAQLGAFAQISGFSFSFDPSKKAGSRVVSITKDGNPMDMDATYKLAVQDYNA
                     IGGDGYSMLVGAKVVYKSSEWNRDGMVAYIMAHPEINPTVEGRITVVKP"
     gene            2642..3520
                     /locus_tag="VN478_04760"
     CDS             2642..3520
                     /locus_tag="VN478_04760"
                     /inference="COORDINATES: protein motif:HMM:NF013514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3378 domain-containing protein"
                     /protein_id="HWQ22015.1"
                     /translation="MNTVKSMSHEQMERLRSALTAIGAVEASELPAGTCFVSRIGGSV
                     TTGYRSGKVLFQGRDIERQVSLAQEILAHEQPASRELEFPIVGGDESGKGDLFGPLVV
                     AAFGARDELERRQVVQAGARDCKLMTDSDVRTVAVRLSRIGVSSVRILMPDEYNAHYA
                     RVHNVNILLNDVYGVLLLELATACKARTVILDKYGGRAKALWKTQQDFHFVVEAHAEQ
                     YPEVAAASVLARAAFLDGLERTARSGGINRLPKGASMEVQAFMRRLSSERGKDVLRSI
                     AKVNFAPVKECLDSLF"
     gene            3618..>3851
                     /locus_tag="VN478_04765"
     CDS             3618..>3851
                     /locus_tag="VN478_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014452912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HWQ22016.1"
                     /translation="MFEPVLPADKVVEQEVATIQFWESAHVFERSLELNKDKERFVFF
                     EGPPTANGRPGVHHGLARVFKDTVLRYKAGTGYY"
BASE COUNT          869 a         1083 c         1137 g          762 t
ORIGIN      
        1 gcgggcggcg gcggattcct tggcttcaag ggcgccccga tcgtctacca gtccagtgat
       61 gagatccgca acctgatgat ccagtacgtc aaagacgctg ggacgattga tccaaccgtc
      121 gatgacaact ggcacgtcat cccggactac ctcagttcgc aataccgcgc agctatcgac
      181 acactcaaca ggcagagtgc cctcagtgaa ttcccgacag cggcctttgc ccctgaatcc
      241 atcgctaatc gcgccgatct cgcggcgatc ctcactggat cctatacctc agttggcagc
      301 cgcgaccatg gaagctcctt cctcgacgta gccagcgaca gctggtatgc ccgagcggtc
      361 gggaccgttg aggctctgaa gatgatgaat ggcacgtcgg ccaagacgtt cactcccgac
      421 gctccagtca cgacagaaca agcggtaacc gcccttatga gggcgagtgg gtacggatac
      481 gaggcttcca ggtatgacct gtgcgcacag cctctggaca aggttcccaa cctcttccgc
      541 aataccgacg ggacgtggaa ctgggaggct ctggctgcag acacgaagaa gaatggcgga
      601 gcgaccatca acatgcgttc cggccagtct ctgggtggcg ttgagatgta cgccatcgct
      661 cttcctgtgg cacctgggat ggtagtcaac ggctatccga ccggagcaga tctcaaagcg
      721 ttcaccatga agaacgccaa gtggctgctg gaccgcaccg gttatgccct cggaacgtgg
      781 tactacaagg gaaagaccta catcgacgta agcacgacga tcccgtggcg tgactacgca
      841 atcacgatgg gcaaggaata tgaccagatc tcgatcttcg accttgccaa ttttgaggag
      901 attcccacgg gcggcacgag cggcaagtcc ggcaagaaac ccaatatgaa cctcatcatc
      961 tcggactggg cgctgccata tgtagctttt gcggaatcga agggcttcat caccgccgaa
     1021 caagccactg cgccgaagca tcaactgacc agaggcgaac tggctgatat ggtcgtgaag
     1081 atgcgtttcc cgacggtggt catcctgcac actaacgatt tccatggcaa cctggttcct
     1141 gtgatcgatg ccaacaagaa cagcgtcgca ggtgccgctc gtgaggctac catcatcaac
     1201 accatgcgca gcacgttcgg agcggatcat gtcctgcttg tggacgccgg cgacgctatc
     1261 cagggcgcca cagttgcaaa cctgttccag ggaaagagcg tgaccgacgc ctacaacgcc
     1321 atgggctaca acgtggcgac actcggaaat cacgagtggg actatggtca ggaagtcctc
     1381 aaggaacgca tcgcagacgc gaagtttgat tatgtgaact ccaatttgac tggcaccaat
     1441 ctgggatgga agtccagcgt catcaagaac atcgctggca tcaacatcgg actgttcggc
     1501 ctcatgacgt cggactaccc gatcatcgtt acgccatcca gccagacgaa tgtcactgtc
     1561 ggggatccga tagcgtcggc caagacagaa gtcgcagacc tcaaggccca gggggcacag
     1621 tacgttgttg ctttgagcca ctgtggctat gaaaacggcg gtgctcctct ggatccagct
     1681 atcgctgccg gggcccctgg catcaatctg atcatcggtg gtcactccca tacggttctg
     1741 aagacagcgg tgatggtcgg caagacgatg atcactcaga cagggactgc cggtgcatac
     1801 gtgggcaagg aagtcatcga cttcacgacg tatcagggaa aggtcactgg acagagtgtc
     1861 tcgtatgagc tcgtgccgac tgtaacctct gttgcagaag atcctgccgt caaggccatc
     1921 gtcgacggat acaacaacca gctgacggcc aagctcagcg tcgtcatcgg caaggctctt
     1981 gttgcactca atggcgagcg cgcggacgtc cgtacgaagg agaccaatct tggcaactac
     2041 gtcacggact ggatgcgcat ctcgaccggc gccgacattg catttatggg cggtggcagc
     2101 atccgtgtca ccgttcctgc cggggacgtc acggtaggca acgtctatgc cgtgcttcca
     2161 tttgacaact tcctcgaagt ccttgagatc acgggctcca cgctcaaggc ctgccttgag
     2221 aactctgtga gtgcctatcc ggctcaactc ggcgcattcg ctcagatatc ggggttcagc
     2281 ttctcgtttg atccgtccaa gaaggcaggt tcacgtgtcg tcagcatcac gaaggacggc
     2341 aatcccatgg acatggatgc aacctataag ctggccgtac aggactacaa cgctatcgga
     2401 ggcgatgggt acagtatgct ggtgggcgcg aaggtcgtgt acaagtccag tgagtggaac
     2461 cgcgacggta tggttgccta cattatggct catccggaga tcaacccgac ggttgaagga
     2521 cggatcactg tcgtcaaacc gtagcagatc atctgtcgtt tcagcgaggg ggccggcagt
     2581 tgccggcccc ctcgcgtctg accagcgttg cacatctgcc gaaaaaggag acaatccatt
     2641 catgaacaca gtgaagtcga tgagccatga gcaaatggaa cgtcttcgat ctgccttgac
     2701 tgccatcggg gccgtcgagg cctccgagtt gccggcgggg acgtgttttg tctccaggat
     2761 cggcggttcg gtcaccaccg gttaccgcag tggcaaggtc ctgttccagg ggcgggacat
     2821 cgaaagacaa gtcagcctgg cgcaggagat cctggctcac gagcaaccag cgagtagaga
     2881 gctggagttt cccattgtcg gaggagacga atccggtaag ggggatctgt tcggtccgct
     2941 cgtcgtcgcg gcgtttggcg cacgtgacga attggagcga aggcaagtag tccaggcagg
     3001 agcccgggac tgcaagctta tgacggattc agatgtgcgt accgtggcgg tgcgactgag
     3061 ccgaatcgga gtgtcgagcg tgcgcatact catgcccgat gagtacaacg cacactacgc
     3121 gcgcgtgcac aacgtcaata tcctgctgaa cgacgtctac ggcgtgctgc tgctcgagtt
     3181 ggcgacggcg tgcaaagctc ggactgtgat cctggacaag tatggaggac gtgcgaaggc
     3241 gctctggaag actcaacagg atttccactt cgtggtggaa gcgcacgcag aacagtatcc
     3301 cgaggtggcc gcggcctcgg ttcttgccag agccgcattc ctcgatggtc tcgaacgcac
     3361 ggcgaggagc ggtggcatca accggctgcc aaaaggggct tcgatggagg tgcaggcgtt
     3421 catgaggcgc ctgtcgtcag aacggggcaa ggacgttctg cgttccattg caaaagtgaa
     3481 tttcgcacct gtgaaggagt gtcttgacag tctgttctag acgggattcg tgactctcat
     3541 ctggttgaca acgccacccg gcgccatagt atatatgcgt agttccgcag cgaaaccttc
     3601 acccgaggag acacctcatg tttgaacccg tactgcctgc tgacaaggtc gtcgagcagg
     3661 aggtagccac catacagttc tgggaatccg ctcatgtttt tgagaggtcg ctcgagttga
     3721 acaaggacaa ggagcgattc gtcttcttcg aagggccacc gactgcaaac ggacggcccg
     3781 gcgtgcacca cggcctggct cgcgtcttca aggatactgt cttgagatac aaggcgggca
     3841 caggctacta c
//
LOCUS       DATMZD010000230         2081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_107014,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000230 DATMZD010000000
VERSION     DATMZD010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107014"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2081
                     /locus_tag="VN478_04770"
     CDS             <1..>2081
                     /locus_tag="VN478_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWQ22017.1"
                     /translation="SKAQEQAVPEIQKALQGMLTAQDAAVAQAKAAVKKQFDATLAAQ
                     KQQAYAMAEAQATKVITTNPPKELIAAEQQVLATVKKQFDAKLATQKQQAYAQAEAQA
                     TQLLTTNPPAELIAAEQQAEAAAEAAAKQQVDAQFAAQAPGVPLENIPGYTAALATAQ
                     QQAAAVAKAQVDAAALAQAVAQAKAAVDAELAKQQPAMQAALAAAQKQARAQVEATAL
                     KQAIVQAKAVVDAQFAAQKVAMDKALADAQAKAVEAATSAVQKGFLDTAKLTAEQLTA
                     MRQFIALPLVSSITDNNVKADTVQTIINLGKTLPMTGQEKTINVSQTDLDRGIADIRE
                     NGGVIPLPAIGKTLLFLLLVYVLSAFLTFVMQYIMSDVAQRTTYDMRKELDYKLAALP
                     LRYYDMHSNGEILSRMTNDMDTISTTLQQGLTQIIVSVFQILGYIYMMVTISGKLTLV
                     TLATLPLYILTTALIARKSQGFYRAQQVHLGRLSSHAEEMYTGHNVVKAFGHEQDSIA
                     TFETVNNDLYQSGWKAQFMSGIMFPLMNFISNLGYVFIAVLGGVYVTRNLLNLGDITA
                     FIQYSRSFSMPIIQTASIANVIQSTMACAERVFTVLDEPEEQPDASDAIVIAQPKGAI
                     AFDHVDFSYRATEPLIRNMNLDVKQGDTVAIVGPTGAGKTTLVNLLMRFYDIQGGAIT
                     FDGVETRKIGR"
BASE COUNT          486 a          664 c          600 g          331 t
ORIGIN      
        1 ctccaaggcc caggaacagg cagtgcccga gatccagaag gcgctgcagg gcatgctgac
       61 ggctcaggac gcagcggtag cgcaggcgaa ggcggcagtc aagaagcaat ttgacgccac
      121 gctcgctgca cagaagcagc aggcatacgc aatggccgag gcgcaggcga ccaaggtcat
      181 cacgaccaat ccgccgaaag agttgatcgc ggccgagcaa caggtactgg cgacggtcaa
      241 gaagcagttc gatgccaagc tggcgacaca gaagcagcag gcatatgcac aggctgaggc
      301 acaggcgacc cagctgctca caacgaatcc accggcagag ctgattgcag cggagcagca
      361 ggccgaggcg gcagccgaag ctgctgccaa gcagcaggtc gacgctcagt tcgcagcaca
      421 ggcccccggc gttccgctgg agaacatccc aggatatacg gctgcgctcg ccacggccca
      481 gcagcaggca gccgcagtgg ccaaggcaca ggtcgatgct gcagcgctgg cccaggcggt
      541 tgcacaggca aaggcggccg tcgatgccga actggcaaag cagcagcctg cgatgcaggc
      601 tgcactcgct gccgcccaga aacaggccag agcgcaggtg gaagccaccg cgctgaagca
      661 ggcaatcgtc caggcgaagg ctgtcgtcga cgcgcagttt gcagcacaga aggtggcgat
      721 ggacaaggcg ctcgctgacg cgcaggcgaa ggcggtcgag gcggccacca gcgccgtaca
      781 gaagggattt ctcgataccg ccaagctgac ggcggaacag ctcacggcca tgcgccagtt
      841 catcgcgctg ccactcgtga gcagcatcac cgacaacaac gtcaaagcgg acacggtcca
      901 gaccatcatc aatctgggca agaccctgcc catgacaggt caggagaaga cgatcaacgt
      961 ttcgcagacc gatcttgacc gaggcatcgc cgacatccgg gagaatggcg gcgtcatccc
     1021 gctgcccgcc atcggcaaga cgctgctctt cctgctgctg gtctatgtcc tgagcgcatt
     1081 cctcaccttc gtcatgcagt acatcatgtc ggatgttgcc cagaggacga cctatgacat
     1141 gcgaaaggaa ctcgactaca aactggcggc attgcccctc cgctactacg acatgcactc
     1201 caacggcgag atcctgagcc gcatgacgaa cgacatggac acgatctcga ctacccttca
     1261 gcagggtctg acacagatca tcgtgtccgt gttccagatc ctcggctaca tctacatgat
     1321 ggtcacgatc agcggcaagc tgaccctggt cacccttgcc acgctgcccc tgtacatcct
     1381 caccacggca ctcattgcac gcaagtcgca gggtttctac cgagcccagc aggtgcacct
     1441 tggtcggctc agcagccacg cagaggagat gtacacgggt cacaacgtcg tcaaggcgtt
     1501 cggccacgaa caggattcga tcgctacctt cgagacggtc aacaacgatt tgtatcagtc
     1561 cggctggaaa gctcagttca tgtcaggcat catgtttcca ctcatgaact tcatcagcaa
     1621 cctcggctat gtcttcatcg cagtccttgg aggtgtctat gtcacgagga atctcctcaa
     1681 cctcggcgac atcaccgcat tcattcagta ctcacggtcg ttctcaatgc ccatcatcca
     1741 gacggcgagt atcgccaacg tcattcagtc gacgatggca tgcgccgagc gcgtattcac
     1801 ggtgctcgac gagcccgagg agcagcccga cgcgagcgat gcgatcgtca tcgcccagcc
     1861 gaagggagct atcgccttcg atcacgtcga cttcagctac cgcgcgaccg aaccactgat
     1921 caggaacatg aaccttgacg tgaaacaggg agataccgtt gccatcgtcg gacccaccgg
     1981 ggccggcaag acgacgctcg tcaacctgct catgcgcttc tacgatatcc agggcggcgc
     2041 catcaccttc gacggcgtcg agacacgaaa gatcggccgc g
//
LOCUS       DATMZD010000231         2003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_46404,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000231 DATMZD010000000
VERSION     DATMZD010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46404"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1087)
                     /locus_tag="VN478_04775"
     CDS             complement(<1..1087)
                     /locus_tag="VN478_04775"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWQ22018.1"
                     /translation="MFYLSNILGMRVLDRKQAVVGSIADLEVATGEKFPAVMAVMVRT
                     RRGLTRVAWHNVRGLGITECTLKLADAELQPNFELQDTSMLLLNHVLDKQIVDIHGAK
                     VVRVNDIELAESHGQLRVIAADAGFRSFMRRAGFAWFVHLVERRHRHKISENSIPWSF
                     VEPLGRDVRSVQVRATWKELSKLHPSDLADIVDDLDFNERDALFKALNDEQAADTLAE
                     LEDDKVKVTILERLGVSRAADILEEMDPDDAADILQDTRAETQEAMLQEMDPEEREDV
                     RELLAYDEDTAGGLMTNDYLEIRQEMSAEQVIGLLRREAPDTETIYYLYVTDDDGHLA
                     GILSLRALIVAQPGTVVGDIMQKRTVSV"
     gene            complement(1206..1901)
                     /locus_tag="VN478_04780"
     CDS             complement(1206..1901)
                     /locus_tag="VN478_04780"
                     /inference="COORDINATES: protein motif:HMM:NF013703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HWQ22019.1"
                     /translation="MGASGTSFGFIVLTGIIGRVPNATVALSAYGIGDRMFSITSLIN
                     DGAGVGIATMVGQALGAGMKDRATAVVRQGTTAMVGLMAIASVLLRLMTPLVFRAFIP
                     SQPDIIAEGVLFMSIFLWSNPFFGLMMGVQSAFQGSGHNVPNMILDLVRLWGLRVPLA
                     WFFAMALKLGSRGVWIGMTISNIASGLLSLVLLLTVDWRKNVIERLPVPETLLLSGEP
                     ISSVSGGEVDDRP"
BASE COUNT          398 a          629 c          589 g          387 t
ORIGIN      
        1 agacagagac agtccgcttc tgcatgatat cgccgacgac agtgccgggt tgtgccacga
       61 tcagcgcccg aagggaaagg atccccgcca gatgcccatc gtcatcagtc acgtacaggt
      121 agtagatcgt ctcagtgtcc ggggcctcgc ggcgcagcaa cccaatgacc tgctcggccg
      181 acatctcctg cctgatctca aggtagtcat tggtcatgag gccgcctgca gtgtcctcat
      241 cgtaggccag cagttcgcgg acatcttcgc gctcttcagg atccatctcc tgaagcatcg
      301 cttcctgagt ctcggcgcgt gtgtcctgaa ggatgtccgc ggcatcgtcc gggtccatct
      361 cctcaagaat gtccgccgca cgggagacgc cgagccgttc caggatcgtg accttgacct
      421 tgtcatcctc cagttctgcc agcgtgtcag ccgcctgctc atcgttgagc gccttgaaca
      481 gcgcgtcgcg ctcattgaag tccaggtcgt ccacgatgtc cgccaggtcc gagggatgca
      541 gtttcgagag ttccttccac gtcgccctca cctgcacgct gcgtacgtcc ctgccaagag
      601 gctccacgaa actccacgga atcgagttct cgctgatctt gtggcggtgt cgtcgttcga
      661 cgagatggac gaaccacgcg aaccccgctc tgcgcatgaa gctcctgaac ccggcgtctg
      721 cagctatgac acgcagctgc ccgtgactct cggccagctc gatgtcgttg acgcgcacga
      781 ccttggcccc atggatgtcg acgatctgct tgtcgaggac gtggttcaac aggagcattg
      841 aagtgtcctg cagctcaaag ttgggttgaa gctccgcatc cgcgagcttg agagtgcact
      901 ctgtgatgcc caggccccgc acattgtgcc atgctacacg cgtcaggccg cgtcgtgtcc
      961 tgaccatgac agccatgacc gccggaaact tctcgccagt tgctacttca aggtcggcga
     1021 tggagccgac gacggcctgc tttctgtcca gaacgcgcat gccaagaatg ttgctgaggt
     1081 agaacatcgg cgcctcctgg ggcgagagaa ttgcacagcg cctgtatcct acgcacgatg
     1141 gctcacggtg tcaagctgac gcccggagac gcgctcttcc gacggcgaac tacagtacag
     1201 atgccttacg gacggtcgtc gacctcgcca ccggagacag acgatatggg ttctccgctg
     1261 aggagcagcg tctcaggcac gggcaggcgt tcgatgacgt tcttgcgcca gtccacggtc
     1321 aacagaagaa caagcgagag caagcccgac gctatgttgc tgatcgtcat gccgatccag
     1381 acgccacgcg aaccaagttt cagggccatg gcgaagaacc aggccaatgg cacgcgcagg
     1441 ccccacagcc gaaccaggtc caggatcatg ttggggacat tgtggcccga gccctgaaat
     1501 gccgactgta cgcccatcat gagcccaaag aagggattgg accagaggaa gatggacatg
     1561 aacaggacgc cttcggcgat gatgtcgggc tgactgggga tgaaggcccg gaacaccagg
     1621 ggagtcatga gtcgcaggag aacactcgct attgccatca ggccgaccat tgctgtcgtc
     1681 ccctgtcgca ccactgccgt ggcgcggtcc ttcatgccgg cacccagcgc ctgtcccacc
     1741 attgtggcga tacccacccc ggctccgtcg ttgatgagag acgtgatact gaacatcctg
     1801 tccccgatcc catacgcaga cagcgcgacc gtggcattgg ggacacggcc gataatgcct
     1861 gtcaggacga tgaacccgaa ggacgtgccg gatgcgccga tcgacgcaga gaaggcggcc
     1921 ggtcagacga tgtctcttgc gatcctgctt ggatttgcgg tggcggtcgt cggtgtcgta
     1981 ctgtcgccga tcctgctccc agt
//
LOCUS       DATMZD010000232         2851 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_46466,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000232 DATMZD010000000
VERSION     DATMZD010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46466"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1238)
                     /locus_tag="VN478_04785"
     CDS             complement(<1..1238)
                     /locus_tag="VN478_04785"
                     /inference="COORDINATES: protein motif:HMM:NF019317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22020.1"
                     /translation="MDKQSSMVHFLRSIRHALVAQSARFRRTGALVFATVALLSAALC
                     ALPFLPVHTSVSEGTVVDADMISRRDIVYVDSAKTLDLRKRASDEVAPIYKFDGTRLP
                     AALTAFEDMMTKVIAIRGQDVGVSLQSERIAALLMTSAGTTTKYLATCSESEVQSSKT
                     AARQALATVMGRGVLEGSVAAARESINAELKKYGISDSQVRSAYLLAVRLVSSNLMYD
                     QEATLLAREKAASLVSPVVSTIIAGTSVVKRGTVLDQGTIDLLLSTGLIWGPRAFRNL
                     LSRMLVALAIWGIVALTIVLLDSPRVRTQHLFMEAGVIGGSCIVIGWLASGMAPTMTP
                     FFLCMLLGLVFFDGALATVFGLALIVVTWVMVPMPAEVLSGLVVGAVSVLVVMRKANK
                     MSALLIAGPVAGVTAGLIYGA"
     gene            complement(1222..1830)
                     /gene="rsmD"
                     /locus_tag="VN478_04790"
     CDS             complement(1222..1830)
                     /gene="rsmD"
                     /locus_tag="VN478_04790"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:NF015559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HWQ22021.1"
                     /translation="MIVGSGTLKGRSLKTLGKEPWLRPTSDKAREAVMDILRPILDGA
                     RFVDICSGTGAVGIEALSNGAAHVTFVDADSRSVRLIRDNVKLLGLEDRCSIVKGDAA
                     GYVSGLQGTEFDIFFADPPFDSGLQEQLVSILAQHLHGAADGCIIVMEHGSRQPLGAR
                     YSGVEVDLENYKERRYGDVSMAFFRATRRRNEADSGGDGQAE"
     gene            complement(1832..>2851)
                     /locus_tag="VN478_04795"
     CDS             complement(1832..>2851)
                     /locus_tag="VN478_04795"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HWQ22022.1"
                     /translation="LMAVATGEIDCLVGTHAILEEGIVFARLGLSVVDEQHRFGVAQR
                     TALGVKGNEPHSLVMSATPIPRTLALTIYGDLDISELLEKPAGRSPVHTHLVAAAERE
                     SAYALVRREVAQGRQAFVVVPAIDQSSDEGDDITSVEETMKRLVEGSLRGLRVASLHG
                     RMKSGEKQSIMDAFRSGTLDVVVATTVVEVGVDIPNASVMVIEDAERFGLATLHQLRG
                     RVGRGSLASFCILIQGRVTADSTQRLQVLVDSSDGFEIAEEDLKLRGPGDILGTVQHG
                     YDVPLFGSTALLTYRDLAALPEIRKVASELLEGDPGLGAPGHGDLARMLSLRYPPSRP
                     GVEDS"
BASE COUNT          602 a          918 c          786 g          545 t
ORIGIN      
        1 gctccatata tgagcccagc cgttacgccg gcgaccggcc cggcgatcag aagcgcgctc
       61 atcttgttgg ccttccgcat gacgacaagc accgaaacgg ctcctactac aagccctgag
      121 agtacttcgg ccggcatcgg caccatgacc catgtcacaa cgatgagagc aaggccgaac
      181 accgtagcaa gggcgccatc gaagaagaca agccccagca gcatgcacag gaagaacggg
      241 gtcatcgtcg gcgccatgcc actcgccagc cacccgatca cgatacacga gccgccgatg
      301 acgcctgctt ccatgaacag atgctgtgtc cggactctgg gtgaatccag caggacgata
      361 gtgagtgcga caatacccca tatggccagc gcgacgagca ttcgtgacaa gaggttgcga
      421 aacgctcgcg gcccccagat cagccctgta ctcaggagca gatcgatggt cccctgatca
      481 agcacagtgc ctctcttcac aacggaagtg ccggcgatga tggtcgagac gaccggcgac
      541 accaacgacg cagccttttc gcgcgcaagc agggtcgcct cttgatcgta catcaggttg
      601 gacgacacca ggcggacagc aagaagatac gccgaacgca cctgactgtc ggaaatccca
      661 tacttcttga gttctgcgtt gatgctctcg cgagctgcag cgacgcttcc ttcaagcacg
      721 ccacgaccca tgaccgtcgc caatgcctga cgcgcagccg tttttgacga ttgcacctcg
      781 gactcagaac aggtcgccag atacttcgtc gtcgtaccag cggatgtcat gagcaatgca
      841 gcaatacgct cagactgcag gctcacgccg acgtcctgcc cgcgtatggc gatgaccttc
      901 gtcatcatgt cctcgaaggc agtcagtgcc gcgggcagtc tcgtgccgtc aaacttgtag
      961 atgggagcga cctcatcgct cgcgcgcttg cgaaggtcca gagtcttggc actgtcaaca
     1021 tagacgatgt cccgtctgga gatcatgtcg gcgtctacaa cggtcccttc cgacacgctc
     1081 gtgtgcacgg gcaggaaggg gagggcgcag agagcggccg acagaagcgc taccgtggca
     1141 aacacaagtg cgccggtccg cctgaacctc gcggactgtg cgacaagagc gtgacgaata
     1201 ctcctgagga agtgaaccat gctactctgc ttgtccatcg ccgccactgt ctgcctcatt
     1261 tctgcgtctg gttgcacgga agaatgccat gctgacgtct ccgtagcgcc tttccttgta
     1321 gttctccaag tctacctcga cgccgctgta ccgcgcacct aaaggctgcc ttgatccatg
     1381 ttccatgaca atgatacagc cgtctgcagc cccgtgcaaa tgctgggcca ggatactgac
     1441 aagctgttcc tgcagtcctg agtcgaaagg ggggtcggcg aagaagatgt cgaactcagt
     1501 cccctgcaat ccactcacat accctgctgc gtcacccttc acgatggagc accggtcctc
     1561 aaggcccagc agcttcacat tgtcacggat gagacgtact gatcggctat ctgcgtcgac
     1621 aaacgtcaca tgagcggctc cgttgctcag tgcttctatg ccgaccgcgc cggtgccgct
     1681 gcatatgtcg acaaaacgcg cgccgtccag gatgggtcgc aatatgtcca tgacagcctc
     1741 acgcgccttg tcggatgtgg ggcgcagcca tggctccttt ccgagagtct tcagcgagcg
     1801 tcctttcagt gttcctgatc cgacgatcat atcagctgtc ctccacgcca ggtctcgacg
     1861 gagggtatcg cagggacagc atgcgcgcga ggtccccatg tccaggggca ccgagtcctg
     1921 gatcgccttc gagcaattcc gaggctacct tgcggatctc cgggagagcc gcaaggtcac
     1981 gataggtcag gagtgccgtg gacccgaaca aggggacgtc gtatccgtgc tggaccgtcc
     2041 cgaggatgtc gcccggtcct ctcagcttca agtcttcctc ggcgatctca aacccgtcgc
     2101 tggaatcgac aagcacctgc aggcgctgag tgctgtcggc tgtcactctt ccctgtatga
     2161 gaatgcagaa ggaggccagg gatccccggc ctactcttcc acggagctgg tgcagcgtgg
     2221 ccagcccaaa cctctctgca tcctcaataa ccatgacact cgcatttgga atgtcgacgc
     2281 cgacctctac aacggtcgtt gcgactacga cgtcaagggt tcctgagcga aacgcgtcca
     2341 tgatcgattg cttctcgccg ctcttcattc gtccatgaag cgacgcgacg cgcagtcccc
     2401 tgagactgcc ttccacaagc ctcttcatcg tctcctcgac cgacgtaata tcgtcgccct
     2461 cgtccgaaga ctggtcgatc gccggaacca cgacaaacgc ctgcctcccc tgggccacct
     2521 ctctgcgcac caatgcatat gcactctcgc gctcggctgc cgcgacaagg tgcgtgtgga
     2581 caggagaccg tcctgcaggc ttctcgagaa gctcagagat atccaggtcg ccatatatcg
     2641 tcagggcaag ggtcctcgga atcggagtcg cgctcatgac cagcgaatgg ggttcgttgc
     2701 ccttgactcc cagcgcagta cgctgggcga ctccaaagcg atgctgctcg tcgaccacgc
     2761 tgaggccaag gcgcgcgaac acaatgccct cctcaagaat ggcatgtgtc cccacgagac
     2821 aatcgatctc gccggtggca accgccatca g
//
LOCUS       DATMZD010000233         3336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_137662,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000233 DATMZD010000000
VERSION     DATMZD010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137662"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..1162
                     /locus_tag="VN478_04800"
     CDS             104..1162
                     /locus_tag="VN478_04800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HWQ22023.1"
                     /translation="MRDAPALSVVIPTWNRATMVVDAVESALAQDSPEPIEVIVVDDG
                     SEDDSPAVLIELARRPQPAGRTIRVILRSHRGLTSTMQCGIDAASGAYVSLLDSDDLW
                     ETGRASGLLAEERRLGGNAVVYTNYRKMDSSGALTGRLGFDPRDMRSLPVDTPALRLA
                     FLRSYVSSVFHSHAFPGSICIFPREMLQGHYALPEGAVTQDFWVALVAYLRYAVAYLD
                     VATLRVREHPGQHHALTDANRWTGLVDEQVTTFSAVTELLRADVPAEARLIEVMEARR
                     RLTVLRRSALEGHRLACLIGSVRMVTTVATHPALGSAMLSNVILSISPRLFAWTRYRH
                     GRRRLDDLTRYGRETHGG"
     gene            1155..2150
                     /locus_tag="VN478_04805"
     CDS             1155..2150
                     /locus_tag="VN478_04805"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HWQ22024.1"
                     /translation="MAETAPSMTVVIITKDRPDDIAAISLPSLAAQTCRDFGVLVWDA
                     SADDATRLAVAGFAAIHPSVSISYARARRPGTCCQRNDAMDSCTSGLLFFIDDDVELM
                     PTAVEEMMRVFDADMPEHIAGCQCALAGGPSTKGISGHLRNIWYGFWGMWYDGNRQAL
                     RLTGFNTAFRLASADAAATIPPAMPSRTDLEWLSGCAMAFRREVLVRHGLRFDERLMR
                     FGGYGRGEDVLLSGLLHLRFGYDLACPPSAVAVHHHGAGGHGSDADQPAMVVYNHALI
                     WQELMSARKYSRLALTWARTGLALRYAVPVIVGRGRAEFSSFRSGLRAAHEVAEQ"
     gene            2150..3163
                     /locus_tag="VN478_04810"
     CDS             2150..3163
                     /locus_tag="VN478_04810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWQ22025.1"
                     /translation="MSSKTAISVVIVTRNRPGELACIALPSLAHQTSSPAQVLVWDAS
                     DDMATQGVVTSMQPLPGTSLQYVRAPRQGTPSQRNDAVSQCIGDVILFLDDDTELWPD
                     ALRQLALVFDADTGRRIAGCQCTLVAGPRWKNRTSGVRWLLAHLYRSVFLLDTLSRRQ
                     GFLPSGHTTGAEPLPEVKAVAQAAQGGCERGLQWMWGNCMAWRRSIFATPGARFDEEL
                     QATGSYGFLEDTMFSRQVLRLTGMELVRARAALCMHHEAGGGRADPRRFGAMYAFNYW
                     LLWRRQVRHTPASTLAFLWSQCGLLVGFAARDILSGRFERVRGLIDGWNLIRNRAGRP
                     EGP"
     gene            complement(3129..>3336)
                     /locus_tag="VN478_04815"
     CDS             complement(3129..>3336)
                     /locus_tag="VN478_04815"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HWQ22026.1"
                     /translation="GVTEAGLDDDFLDLGGDSLGATRFIAEVRAVLGCSLGYVELFDH
                     ATVRSLAGLLIDRVRVLPASPPDF"
BASE COUNT          593 a         1058 c         1054 g          631 t
ORIGIN      
        1 gttcatgacg ttcatcatag gcgttcgcgc gcacgcagtc aactagttct ccacacgtag
       61 acaaaagggg cttcctgcat aaggtagttc catggaaggc ctgatgagag atgctcctgc
      121 cctgtcggtg gtgataccga cgtggaacag ggcgacaatg gttgtggatg cagtcgagag
      181 tgcccttgca caggactccc cggaacccat cgaagtcatt gtcgtcgacg acggctcgga
      241 ggatgactcg cctgccgtcc tgatcgagct ggcaaggcgc ccccagccgg ccggccgtac
      301 gatcagggtc attctcagaa gccaccgggg cctgacgtcg accatgcagt gtgggatcga
      361 tgcagcctcg ggcgcatacg tctctctctt ggattccgac gacctgtggg aaacaggacg
      421 cgcatcggga ctgctggcgg aggagagacg cctgggcggc aatgcagtag tctacaccaa
      481 ctacaggaag atggacagct ccggggcact cacgggcagg cttggattcg acccccggga
      541 catgcgatca ctaccggtcg acacaccggc tctgaggctg gcattcctgc gctcctacgt
      601 ctcttccgtg tttcacagcc atgcattccc cggttccatc tgcatctttc ctcgagagat
      661 gcttcaggga cactatgccc ttcccgaggg agccgtcacg caagacttct gggtagcgct
      721 ggtagcgtat ctcaggtatg cggtggcgta tctcgacgtg gcgactctgc gcgtgcggga
      781 gcatccagga cagcaccacg ccctgacgga tgcaaaccgg tggacgggat tggtcgacga
      841 acaggtcacg acattctctg cagtgacgga gctattgcgt gcagacgtcc cggccgaggc
      901 gcgcctcatt gaggtcatgg aggcccgccg gcgcctgacg gtcctgcgcc gatcagcact
      961 ggaggggcat cgcctggcat gcctgatcgg cagtgtgaga atggtgacga cagtggcaac
     1021 acatccagcc ctgggttcgg cgatgctctc caacgtcatt ctgtcgatca gtccccggct
     1081 gttcgcctgg acgcgctacc gccacgggcg tcgccggttg gacgacctga cccgatacgg
     1141 ccgggagacc cacggtggct gagactgctc cctccatgac cgtcgtgatc atcacgaagg
     1201 atcgtccgga cgacatcgca gccatcagct tgccatctct cgcagctcag acgtgccgcg
     1261 atttcggtgt tctcgtatgg gacgccagtg ctgacgatgc gacccgcctg gcagtcgccg
     1321 gtttcgccgc catacacccg tctgtgtcca tcagctatgc acgcgcgcgg cggccgggga
     1381 catgttgtca gcgcaacgac gcgatggact catgtacatc cggcctcctg ttcttcatcg
     1441 acgacgatgt ggagttgatg cccactgccg tcgaggagat gatgcgtgtg ttcgacgccg
     1501 acatgcctga gcacattgca ggctgccagt gtgctctcgc aggcggtccg tcgacaaagg
     1561 gaatatcggg tcacctcagg aacatctggt atgggttctg gggcatgtgg tacgacggga
     1621 accgccaggc attgcgcctg accgggttca acacggcatt tcgccttgcg tctgccgatg
     1681 cagcagccac gatccctccg gcaatgccct ctcgaaccga cctggagtgg ttgtcagggt
     1741 gcgccatggc gttccggcga gaagtccttg ttcggcacgg gttgcggttc gacgaacgcc
     1801 tgatgcgctt cggcgggtac ggccgaggcg aggacgtctt gctgtcggga ctcctccacc
     1861 tccggttcgg ctacgacctg gcctgccccc cctcagccgt ggcagtgcac caccacggtg
     1921 caggcggtca tggcagcgac gcagaccagc cggccatggt cgtctacaat catgcgctca
     1981 tctggcagga actcatgtcc gcgaggaagt actctcgtct cgcactgaca tgggccagga
     2041 ccggccttgc tctccgatat gccgttccgg tcattgtggg gcgcggccgg gccgagttct
     2101 cctcattcag atccggactc cgtgccgcac acgaagtggc tgaacagtga tgagctccaa
     2161 gaccgccatc tcagtggtga tcgtgacgcg gaaccgaccg ggcgagctgg catgcattgc
     2221 cttgccttca ctcgcacacc agaccagctc gcctgcccag gtcctcgtat gggacgcaag
     2281 cgacgatatg gccacacagg gggtcgtcac cagcatgcaa cccctacctg gaacttcact
     2341 gcagtacgtg cgcgctcccc gtcaggggac accgtcacag cgcaacgacg cggtctcgca
     2401 gtgtatcggc gacgtcatcc tgttcctcga tgacgacacc gaattgtggc cggacgcgct
     2461 gcgacagctg gcgctcgtgt tcgatgcgga taccggtcgg cgcatagccg gctgccagtg
     2521 cacactggtt gccgggccca ggtggaagaa caggaccagc ggcgtgcggt ggcttctcgc
     2581 acacctctat cgctccgtgt ttctcctcga cacgctttct cgccggcagg ggttcctgcc
     2641 ctcaggccat acgaccggcg ccgagcctct tcctgaggtg aaggcagtgg cccaggcggc
     2701 acagggaggg tgcgaacgtg ggctgcaatg gatgtggggc aactgcatgg cctggcggcg
     2761 atcgatcttc gccactccgg gtgcaagatt cgacgaggag ctccaggcca cgggttccta
     2821 cgggttcctc gaggacacga tgttctcgcg gcaggtcctg cgcctcaccg ggatggaact
     2881 ggtccgcgcc agggccgcgc tgtgcatgca ccatgaggca gggggaggtc gtgcggaccc
     2941 ccgccgcttc ggcgccatgt acgcgttcaa ctactggctt ctgtggcgcc ggcaagttcg
     3001 tcacacacct gcgtccaccc tggccttcct gtggtcgcag tgcggtctgc tggtcgggtt
     3061 tgccgctcgc gacatcctgt ccgggcgctt tgagcgtgtg cgcggcctga tcgacgggtg
     3121 gaatctgatc agaaatcggg cggggaggcc ggaaggaccc tgacacggtc gatgagcagg
     3181 ccagcaagac tgcgcaccgt cgcatgatcg aagagctcga catagcccag agaacagccg
     3241 agcacggccc gcacctcagc gataaagcgc gtcgcgccca gcgaatcgcc tcccagatcc
     3301 aaaaagtcgt cgtcgagtcc agcctctgtc acgccg
//
LOCUS       DATMZD010000234         8509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_147054,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000234 DATMZD010000000
VERSION     DATMZD010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8509
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147054"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1492
                     /locus_tag="VN478_04820"
     CDS             <1..1492
                     /locus_tag="VN478_04820"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HWQ22027.1"
                     /translation="GTVFRDKGLKAVVIHFSGTTPTLNCPADMEPIKLAGGRMNKEIL
                     TLDHTHNRMREIGTGNTVEILDKCDVLPVMNYKFGSDPRTEKIKSDVWLGMITQGMPD
                     GCWLGCSMSCAHGVDNFELLTGPLKGQKVLVDGPEYETIGGLGSNTGSFNPHFVLEAN
                     FYCDYYGIDLVAVATLLAFLMECYENNVITKEITGGLDLHFGSDMDALELIHQIGQGR
                     GFGKIAGLGIHRLKKLFIDEYHADAQFVNDIGMEVKGMEVSEYNCKDSLAQQGGYAMA
                     SKGPQHDEAWLIFMDQVKGQLPTFADKAEALSFFPLFRTWFSLMGLCKLPWNDSEPRD
                     NRDKYTGLEAARIPEHIDNYLLLYKGVTGHDLDLDGLLAQSKRVYDFQRLFNLRMGHG
                     TREGDWMPYRGMGPVTEEEYLSRQERYEKQLTEAAKVDISGLSLQQKMAATRQYREAQ
                     YRQLEDTVYARRGWDADGVPTLETIKADGIDFPELVELIQNHAARD"
     gene            complement(1570..2520)
                     /locus_tag="VN478_04825"
     CDS             complement(1570..2520)
                     /locus_tag="VN478_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HWQ22028.1"
                     /translation="MLTQFSDWFTYTLLRMPEGTLLSGAVHFFVYDIIKIYLLLVVVV
                     FLISLVQTWLSAERIKRILVHRNEALNNVLASLLGAIAPFCSCSSIPLFIGFVESGVP
                     LGVTMSFLVASPMITPQGVVLLWGLFGAKVALLYVACGLVIAIASGWLIGRLHLERWV
                     EDYVYQIQMGKAVSLAAQTFRERVQSSWASVREIFRKVAPWVAVGVAVGAFIHGYAPE
                     AWLARVAGRDNPFAVLIAVVVGIPLYASSEGVIPIVQALWAKGLPLGTTLAFMMAVVG
                     LSLPEIIILRRVLKPKLLATFVGIVGLGIIIMGYLFNWIF"
     gene            complement(2584..2826)
                     /locus_tag="VN478_04830"
     CDS             complement(2584..2826)
                     /locus_tag="VN478_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011908441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HWQ22029.1"
                     /translation="MRIEILGSGCARCHGLEDSVRKALATLGKDAEVVDVTDMQQIMA
                     HGVMSMPALVIDGKVMSAGRVLTPEEAKKAIVGASA"
     gene            complement(2835..3161)
                     /locus_tag="VN478_04835"
     CDS             complement(2835..3161)
                     /locus_tag="VN478_04835"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWQ22030.1"
                     /translation="MEERVPACGTQDIVADWDSECEEQAKLVSVLLEPQRIKVLRLLR
                     AGELCVCEIERSLGIPQNLVSHHLRVLREAGLVAARKEGQFVHYSRVEDRIHELTVAL
                     DKLLSN"
     gene            complement(3265..3486)
                     /locus_tag="VN478_04840"
     CDS             complement(3265..3486)
                     /locus_tag="VN478_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004068023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HWQ22031.1"
                     /translation="MSRTWYPMIDEKLCIGCLSCVAFCPHGVYEEYDGKPRIIHPENC
                     IERCHGCQLHACSSGAISYFGDAPSEFRS"
     gene            complement(3584..4636)
                     /locus_tag="VN478_04845"
     CDS             complement(3584..4636)
                     /locus_tag="VN478_04845"
                     /inference="COORDINATES: protein motif:HMM:NF026253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HWQ22032.1"
                     /translation="MAFPDCGRQIPGSPIVGTGSAAYSTPTRGLPQLIPYRRGKKWGF
                     CDAGKRLVVPAAFYEVNPFSEGLAAVRVSEDQELESRWGLIDASGDFVVPPVYALVGS
                     CSNGLCVVTPDSLTEWDHCGPTVTLGSGGYVDVAGRLIIPVAFQGAEPFSEGLAAVEI
                     HGKWGYIDMQGHVVIPAMYQFAGPFRGGLAPVCVHEKYCFIDVKGIVAIPPRYDAARE
                     PFSEGLAPVKVDGKWGYVDCEGAQTPAVYDWAGPFHDGRAPVQTNGTQGYVDRKGELV
                     LPAIYKSAGPFGDGLAPVEIDGKCGYIDASGSLVLPAVYIWAHPFSEGIALVWTRRER
                     SASGYIDARGNRYWEE"
     gene            complement(4673..5041)
                     /locus_tag="VN478_04850"
     CDS             complement(4673..5041)
                     /locus_tag="VN478_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22033.1"
                     /translation="MAESRVEIEVYGIWDEAPAASSCGCGGGSCGCGAPAPTMGDMFE
                     DLRKSLDASDIKDQCSLRFIDVMKDSGPQVDEVRGLVEKGYTLPLTALNGKWAFYGGM
                     SSEMFYGKIKGLLTGAKPVR"
     gene            complement(5022..5942)
                     /locus_tag="VN478_04855"
     CDS             complement(5022..5942)
                     /locus_tag="VN478_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HWQ22034.1"
                     /translation="MTDVERLLRPGAKGTRQVFFAGKGGVGKTTVACLTAVHAARHGY
                     RTLLVTTDPAAHTGSVLGTIVTEQVAPVDGIPGLSAAKIDQKQATEEYKKKILDDARS
                     KFGEDTIRTMAEELASPCTEEMAAFQRFVDLSGMGGFDVTVFDTAPTGHTLRLLQLPL
                     DWSEQIRLKAGASDGLSEGDLRQEARFDHVIAAMRDPEVTTFSFVMYPESTPILEAQR
                     ASQELAGIGVATQLVVANLLIAEEQATTPFFQKRRQMQLGYLEDMRRRFAGAAVLQVP
                     MMDSDIRGIDKLDKLDKIVFGGADRGRVKS"
     gene            complement(5935..6822)
                     /locus_tag="VN478_04860"
     CDS             complement(5935..6822)
                     /locus_tag="VN478_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HWQ22035.1"
                     /translation="MNPEYVFFSGKGGVGKTSMACTAAVHYADSGKKTLIVTTDPASN
                     LADVFEQPIGHHVTPVTGVPNLWAMEIDPDEATREYKERSLAPMRGLFDEEMITVVEE
                     QLSGPCTEEMAAFDKFTDFMEDDSWDVIVFDTAPTGHTLRLLQLPVEWSRHIKESAEG
                     SGQTCMGPVALIQDSKQKYDDAVARLRDASRTQFIFVMQPEQTSLEETSRSVAELAAL
                     GVATSRIIVNGMIPEAETVNPFFRKRREMQEAYLEQARQLYTGMPVQTMELLDSEIKG
                     VAMFRRVAPLLFPEGGSND"
     gene            complement(6812..7132)
                     /gene="arsD"
                     /locus_tag="VN478_04865"
     CDS             complement(6812..7132)
                     /gene="arsD"
                     /locus_tag="VN478_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite efflux transporter metallochaperone
                     ArsD"
                     /protein_id="HWQ22036.1"
                     /translation="MKIEFFEPAMCCTTGLCGPSVDEHLVRLNEDLKRLQAEDASLVV
                     ERYAINQRPFKFRDNADVYKLVTGNGKKILPITVIAGKIVKTKSYPSYEEMKAALDEG
                     THES"
     gene            complement(7190..7564)
                     /locus_tag="VN478_04870"
     CDS             complement(7190..7564)
                     /locus_tag="VN478_04870"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWQ22037.1"
                     /translation="MIAVARALGDETRARIVALLGVRALCVCELIAALDLSQATVSDH
                     VRILVDAGLLTASKQSYWTVYSVRAGLPAETLQFLVSLAQQVNTAFAGDRTRLTDEPV
                     FLCRTDSPTRAAPRPRTLRRKT"
     gene            complement(7774..>8509)
                     /locus_tag="VN478_04875"
     CDS             complement(7774..>8509)
                     /locus_tag="VN478_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22038.1"
                     /translation="FAVYVDNQYRIQAMRQLSSRWPQSAADFTAQYIGMDLYQLLGTS
                     DQATLAAGGDLSGPGHQVMRTLLGSVYIADFGEYAFSRLVAEHGIQDLHLVKPFPAFA
                     ATSTTGQALDTPGLAGKYTAIVFTDPVCGSCYDVAMELVTTLKARTSGWNVIAVVFGE
                     AEISPVKRFVEEAGEQGAQVIIDPKSAVGEQMGHTDAPYAVLLDKDTNVLYSGNALKQ
                     SPTAGGDAPEQTSVYTVIEDILKVPQ"
BASE COUNT         1787 a         2715 c         2351 g         1656 t
ORIGIN      
        1 cggcaccgtc ttccgggaca agggactcaa ggctgtcgtc atccacttca gcgggacgac
       61 gccgaccctc aactgcccgg ctgacatgga gccgatcaag ctcgcaggtg gacgcatgaa
      121 caaggagatc ctcacactcg accacaccca caaccgcatg cgcgagatcg gcacgggcaa
      181 caccgtcgag atcctcgaca aatgcgacgt cctgccggtc atgaactaca agttcggttc
      241 cgacccgcgt accgagaaga tcaagagcga tgtctggctg gggatgatca cccaaggcat
      301 gcccgacggc tgctggctcg gatgctccat gtcgtgcgcc cacggcgtcg acaacttcga
      361 actgctgacc ggcccgctga agggccagaa ggtcctggtc gacggtccgg agtatgagac
      421 catcggcggc ctcgggtcga acaccggcag cttcaatccg catttcgtcc ttgaagccaa
      481 cttctactgc gactactatg gcatcgacct cgtcgccgtg gcgacactgc tggccttcct
      541 gatggaatgc tatgagaaca acgtcatcac gaaggagatc accggaggcc tcgacctcca
      601 cttcggcagt gacatggacg cgctggagct catccaccag atcggtcagg gaagaggatt
      661 cggcaagatc gccggcctcg gcatccaccg tctcaagaag ctgttcatcg atgagtacca
      721 tgctgacgct cagttcgtga acgatatcgg catggaagtc aaaggcatgg aagtatccga
      781 gtacaactgc aaggattccc tcgcccagca gggcgggtat gcgatggcct ccaagggtcc
      841 acagcacgac gaggcatggc tcatcttcat ggaccaggtc aagggccagc tgcccacgtt
      901 cgctgacaag gccgaggccc tgtccttctt cccgctgttc cgcacctggt tctcgctcat
      961 gggcctgtgc aagctgccct ggaacgactc ggaacccagg gacaaccggg acaagtacac
     1021 ggggctggaa gctgccagga tccccgaaca tatcgacaac tacctgttgc tctacaaggg
     1081 tgtgacagga catgacctcg acctcgacgg cctgcttgca cagagcaagc gcgtctacga
     1141 cttccagcgt ctgttcaacc ttcgcatggg ccatgggacc cgcgagggcg actggatgcc
     1201 gtaccgtggc atgggaccgg tcacggaaga ggagtacctg tcacgtcagg aacgctatga
     1261 gaagcaactg actgaagccg ccaaggtgga catctcgggc ctgagcctgc aacagaagat
     1321 ggcagccaca cgccagtacc gtgaggcaca gtatcgtcag cttgaggata cggtgtacgc
     1381 acgccgcggc tgggacgcgg acggcgtgcc gacgctcgag accatcaagg ccgatggcat
     1441 cgacttccct gaactcgtgg agctgatcca gaaccacgct gcccgggact agagctgacc
     1501 gttcgacaaa gagacaacgc ccggcctcat cgaggccggg cgttctgcgt tccacccaca
     1561 cgacgaagtc tagaagatcc agttgaacag gtatcccatg atgatgatac caagaccgac
     1621 gatgccaacg aaggtcgcca ggagtttcgg cttcagtacg cggcgaagaa tgatgatctc
     1681 cggcagcgac agtccgacaa cggccatcat gaacgccagg gtcgtgccca gtggcaaccc
     1741 cttggcccac agcgcctgaa caatagggat cacgccctca gaactggcat aaaggggaat
     1801 accgacaacg acagcgatga ggacggcaaa tggattgtcc ctgccagcca ccctggccag
     1861 ccatgcctcg ggcgcatagc catgaatgaa ggcaccaacg gcaacaccaa ccgcgaccca
     1921 tggtgccacc ttgcggaata tctcgcggac cgacgcccag cttgactgca cacgctccct
     1981 gaacgtttgc gcagccaacg ataccgcctt gcccatctgg atctggtaca cgtagtcctc
     2041 gacccaccgt tcgagatgca gccgtccgat gagccatccg gatgcgatgg caatgaccag
     2101 cccgcatgca acgtacagca gcgcgacctt ggcaccgaac agtccccaca acaggactac
     2161 gccctgtgga gtgatcattg gacttgcaac gaggaaggac attgtgacac ccagcggcac
     2221 gccggattcc acgaagccaa tgaacagagg gatcgacgag cacgaacaga acggagcgat
     2281 tgcccccagc agggatgcca gcacgttgtt gagagcctcg ttccggtgta ccagaatccg
     2341 cttgatcctc tcagctgaga gccacgtctg aaccagggag atcaggaaca cgacgacgac
     2401 cagcagcagg tagatcttga tgatgtcata gacgaagaag tgaacggccc cgctcagcag
     2461 cgtcccctct ggcatccgga gcagtgtgta tgtgaaccag tcagagaatt gcgtcagcac
     2521 aacaacctcc attgatatat cggtcttcaa aaaagggcgg ttgcccgccc gttggtggtc
     2581 tcgtcaggcg cttgcgccga caatagcctt cttcgcttct tcaggtgtca atactctgcc
     2641 tgctgacatg accttgccgt caatgaccag cgccggcatg gacatgacac cgtgcgccat
     2701 gatctgctgc atatcagtca cgtccacaac ctcggcgtcc ttgccgagcg ttgccagcgc
     2761 cttgcggacg ctgtcctcga gcccatggca acgggcacaa ccagacccca gtatttcgat
     2821 acgcatgatc cctcctagtt cgacaacagc ttgtccaacg ccaccgtcaa ttcatgtatc
     2881 cgatcttcaa ctcgcgagta gtgcacgaac tggccttcct tgcgagcagc gacaagcccc
     2941 gcttcccgca agactctcaa gtgatgcgaa acaagattct gcggaatgcc aagtgaccgc
     3001 tcgatctcgc agacgcacag ctctccagct ctcagaagcc tgaggacctt gatccgctgc
     3061 ggttcaagca ggactgacac gagcttcgcc tgctcctcgc actccgagtc ccagtcggca
     3121 actatgtcct gtgttccaca ggcaggaacg cgttcctcca tatcacaact cccgttgatt
     3181 caatctacat tgatatgtta tccagacgtt cagatttgtc aagacccagg caccaaacat
     3241 accaggaatc cctcccagtg gcaactacga tcggaattcg ctcggtgcat ccccgaagta
     3301 gctgatcgca cccgacgagc atgcgtgcag ctggcatccg tgacagcgct cgatgcagtt
     3361 ctcaggatgg atgatgcgtg gtttgccgtc atattcctca tagacgccgt gcgggcagaa
     3421 tgcaacacat gacagacagc cgatgcagag cttctcatct atcatggggt accatgtcct
     3481 ggacatttcg agcctccagt atgtcggatc atcttgctga gactgcaaag cgacgcagag
     3541 ttgaatcctc ggcggggtga ctcgggtcgg tgagtatctc tcgtcactcc tcccagtatc
     3601 gattcccacg tgcatcaatg taaccggaag cagaccgttc tctgcgtgtc catacgagtg
     3661 cgatgccctc actgaaagga tgcgcccaga tgtagactgc tggcaggacg agacttccgc
     3721 tcgcgtcgat atagccgcac ttgccgtcga tctcgactgg ggccagacca tcgccgaagg
     3781 gacctgccga cttgtagatc gctggcagga ccagttctcc cttcctgtcg acatagccct
     3841 gtgtgccgtt ggtctgaacg ggcgcacgac catcatggaa aggacccgcc caatcgtaga
     3901 cagccggtgt ctgtgctccc tcgcagtcga cgtaacccca cttgccgtca accttgacgg
     3961 gggccaggcc ttcgctgaat ggctcccttg ccgcatcata ccgtggcgga atcgcgacga
     4021 tccctttcac gtcgatgaaa cagtacttct catggacaca gaccggcgcg aggccgcccc
     4081 tgaacggtcc ggcgaattgg tacattgctg gtatgaccac gtgtccctgc atatcgatat
     4141 agccccactt cccatggatc tcgacggctg cgagtccttc gctgaagggt tccgctccct
     4201 ggaaggcgac cggtatgatc aggcgccccg caacgtcaac gtatccgcct gaacccagtg
     4261 tcaccgtggg accgcagtga tcccactccg tgagggagtc gggggttacc acgcacaagc
     4321 cattgctgca ggaacccacc agggcataga ccggcggcac gacgaagtcc ccgcttgcgt
     4381 cgatgagacc ccacctggat tccagctcct ggtcttcgct cacgcgcacg gccgcgagac
     4441 cctcgctgaa ggggtttact tcatagaagg cagccggcac gaccaggcgc ttgccggcgt
     4501 cgcagaagcc ccacttcttg cctctccgat agggaatgag ctgggggagc ccccgcgtcg
     4561 gcgttgaata tgctgcactc ccggtgccga cgatcggact gccaggaatc tgccttccac
     4621 aatctggaaa cgccatcagt cgcctccttc agcgcacaga tgttcctctc attcagcgaa
     4681 ccggcttcgc tccagtcagg agtcctttga tcttgccgta gaacatctca ctggacatgc
     4741 caccgtagaa cgcccacttg ccattgagcg ccgtcagcgg cagggtgtat cccttctcga
     4801 ccagcccacg gacctcgtct acctgaggac cgctgtcttt catgacatcg atgaatctca
     4861 gcgagcattg gtccttgata tcacttgcat cgagggattt cctgagatcc tcgaacatgt
     4921 cgcccatcgt cggtgccggt gcgccgcagc cgcagctacc tccaccacac ccacacgatg
     4981 aagctgccgg cgcttcatcc caaatgccat aaacctcgat ctcaactctt gactctgcca
     5041 cgatccgctc ctccaaatac gattttgtcc agcttgtcca gcttgtcgat accgcgaatg
     5101 tcactgtcca tcatcggcac ctgcaatacg gcagctcctg caaaccgtcg gcgcatgtct
     5161 tccaggtatc ccagctgcat ctggcgacgc ttctggaaga acggcgtcgt ggcctgctcc
     5221 tcggcgatca gcaggttcgc cacgaccagc tgcgtcgcta caccgattcc cgccagttcc
     5281 tgagatgcac gctgggcctc aaggatcggc gtgctctcgg gatacatgac gaaactgaag
     5341 gtcgtcactt ccggatcccg catcgcggcg atcacgtggt caaaccgggc ttcctgccgg
     5401 aggtctccct cagataagcc gtcggaggca cctgccttga gcctgatctg ctcgctccaa
     5461 tccagaggaa gctggaggag tctcaacgtg tgccccgtcg gcgccgtgtc gaagacagtg
     5521 acatcgaacc cacccatccc cgacaagtcg acgaaccgct ggaaagccgc catctcctcg
     5581 gtacagggag atgccagctc ctctgccatc gtccggatcg tgtcttcgcc gaacttcgac
     5641 cgggcatcgt ccaggatctt cttcttgtac tcctcggtcg cctgcttctg gtcgatcttg
     5701 gccgcggaca acccgggaat gccgtcgaca ggtgccacct gctctgttac gatggtccca
     5761 agcacgctgc cggtatgcgc tgcagggtct gttgtgacca gcagggtccg gtacccatgt
     5821 ctggcagcgt ggacagcagt caggcatgcg acagtcgtct tgccgacacc acccttgccc
     5881 gcgaagaaca cctgtctcgt acctttcgca ccgggacgca gcaggcgctc gacgtcagtc
     5941 attgctgcct ccctcaggga acagcagcgg agccacgcgg cggaacatcg ccactccctt
     6001 gatctcgctg tcgagcagct ccatcgtctg gacgggcata cccgtgtaca actgcctcgc
     6061 ctgttccaga taggcctcct gcatctcccg cctcttccgg aagaaggggt tcacggtctc
     6121 ggcttcaggg atcatcccat tcacgatgat cctcgaggtc gcaactccca acgccgccag
     6181 ctcagcgacc gagcgagagg tctcttcaag cgatgtctgc tcgggctgca tcacgaaaat
     6241 gaactgcgtc ctgctcgcat cacgcagtcg ggccaccgca tcatcgtatt tctgcttgct
     6301 gtcctggatc agggcgaccg ggcccatgca ggtctgaccg ctgccttcgg cgctctcctt
     6361 gatatgcctg ctccattcga ccggcagctg cagaagtcgg agggtatgcc ccgtcggggc
     6421 cgtgtcgaac acgatgacat cccagctgtc gtcctccatg aaatcggtga atttgtcgaa
     6481 ggccgccatc tcctcggtgc atggaccgct cagctgctcc tcgacaaccg tgatcatctc
     6541 ctcgtcgaat agcccacgca tgggagccag tgaccgttcc ttgtactcac gggtagcctc
     6601 gtccgggtcg atctccatgg cccagagatt cgggacacct gtgacgggtg tgacatgatg
     6661 cccgatgggc tgctcaaaca cgtcggccag gttggatgcc ggatccgtcg tgacgatcag
     6721 cgtcttcttg ccgctgtcgg cgtagtgaac ggccgccgta cacgccatcg acgtcttgcc
     6781 cacgccgccc ttgcccgaga agaagacgta ctcaggattc atgcgtccct tcgtcgagcg
     6841 cggccttcat ctcctcataa gatgggtaac tcttcgtctt gacgatcttg ccggcaatga
     6901 ccgtgatggg caggatcttc ttgccgttgc ccgtcacgag cttgtagacg tcggcattgt
     6961 cgcgaaactt gaacggccgc tggttgatgg cataccgttc cacgacgagg ctggcatcct
     7021 ccgcctggag ccgtttcaaa tcctcattga ggcgcacaag gtgctcgtca actgacgggc
     7081 cgcagagccc cgtcgtgcaa cacatagctg gttcaaagaa ctcgatcttc aacgtgacct
     7141 cctggcaacg aatgtcgtga agatgtgagt gaggcgattc agccgcgcgt caggtcttcc
     7201 ggcgcaacgt gcgcggacgc ggcgctgctc tggttggcga atcggtacgg cacaggaaga
     7261 ccggctcgtc cgtcagacgc gtccgatctc cagcgaaagc agtgttgact tgctgggcaa
     7321 ggctcaccag aaactgaagt gtctccgccg gaagacccgc ccggacggag tagacggtcc
     7381 agtaactctg tttcgaagcc gtcagcagcc ccgcgtcgac aaggatccgg acgtgatccg
     7441 acacggttgc ctgtgacagg tcgagagctg ctatcagctc gcagacacac agcgcacgca
     7501 cgccgagaag ggccacgatg cgcgcgcgcg tctcatcccc cagcgctctc gcaacggcaa
     7561 tcatatctcc aggtccacca ctgttcatct gtgtcaccat ctccggccga cttctctatg
     7621 ggataactat atcgtcatat tccgatttag agtagacatg cctttgtgag tgtcaagaac
     7681 ggggttctct ctgaaaacaa gagacggcag acgaccccgg ggatcagtct cacgaggacg
     7741 cgggtgcgac accacagaat ctctgacggt tgactactgt ggcaccttca ggatgtcctc
     7801 gatcaccgtg tagacggagg tctgttccgg ggcgtcgccg ccggcggtcg ggctctgctt
     7861 cagagcatta ccgctgtaga gaacgttggt gtccttgtcc agcaggacgg catacggggc
     7921 atccgtgtgt cccatctgct ctccaacagc actcttcggg tcaatgatga cctgggctcc
     7981 ctgttcaccg gcctcctcca caaatcgctt cacgggagag atctcagcct ccccgaacac
     8041 gaccgcgatc acgttccagc cggaagtccg cgccttgagc gtcgtgacaa gttccatggc
     8101 gacgtcgtag cacgatccgc acacgggatc ggtgaaaacg atagcggtgt acttgcctgc
     8161 aaggccggga gtgtcgagcg cctggcccgt cgtgctggta gccgcgaaag caggaaaagg
     8221 cttcacgaga tgcagatcct ggatgccgtg ctcggccacc agcctgctga atgcatactc
     8281 tccgaagtcg gcgatgtaga cagaacccag gagtgtgcgc atcacctggt gtcccggacc
     8341 tgacaaatcg cctccggcag caagggtggc ttgatcactc gtgccaagaa gctgatacag
     8401 gtccatcccg atgtactgcg ctgtgaaatc ggcagcggac tgcggccagc gggagctcag
     8461 ctgacgcatc gcctggatcc tgtactggtt gtcaacatag acggcgaac
//
LOCUS       DATMZD010000235         8606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_46524,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000235 DATMZD010000000
VERSION     DATMZD010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8606
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46524"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1254)
                     /locus_tag="VN478_04880"
     CDS             complement(<1..1254)
                     /locus_tag="VN478_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22039.1"
                     /translation="MDTTRIARLIIANTACFLAFWIARLAVPWWFGIPALVVLNIALP
                     HQNQRLFRLYHGLETCLIIVYLAMTADRHTLQELLAILLLTVLFELYFWLVGNLTLAA
                     GSPAHFLRISIVACCYAVLGNTDPLVATVVLAIMLAASMLSAVTASDPRTARRLLPGF
                     IILLIVFVAAVASPIVQAPTLPRAAVWMLPGTPASSPKPAGTGGIPVNLPGLTLRRVM
                     EEHFWELFGFMYLPLAIMVAVLIAAVIVSVYVLHNSWGKTMRALIFPTAIVVSALVFT
                     IIYTATKTAQISGGITALGAPLQNIDDVVEALRQRDILPPTKNEMKATLETIVAFAYW
                     IGASILLVSIVASIRDMVFEFRHEVMLSMLDRSERKKVIRAVRRLVSLNDATLMEDPI
                     TSITAMFAMGVEAVGTVGPRLRRGET"
     gene            complement(1265..2572)
                     /locus_tag="VN478_04885"
     CDS             complement(1265..2572)
                     /locus_tag="VN478_04885"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HWQ22040.1"
                     /translation="MDTAGVHQMKALPQGRSRTPGRPGGLRVFFRTAGTGLLASFLML
                     GVARLLRSASFLIVGLAALSYTVLLCGMLTSQHQRLAVTSVLRHSVATVRQPNMLTCT
                     ASGYRVAQPLLLSVEPLLPPYVVQRHSTHADDGTTVFFDATRKGQYAMGAARVSLQDP
                     LRIFSIHSLIPSGLVLTVVPRPLSLAAMHIALTSPIDGQRVRYAPNVDTSQLTGTHPY
                     DGEPMNRIHWKMSAHTGTLVVKDFTPSASQTVVLLVNYTVHRDTGFTLDVLDETLSTA
                     AASILHYVHDRNLPFGLAAIGSRAYWTGTGHDRMHLLSCLTAVARSQAAQTEDTANVL
                     DWLNQNARAIPPQSQLVLLAHEMDETEVVHLLRQREHFARITIVLFPEGTFLMPGEHR
                     APYYLKDTSKLLRMRSLQHVLRENGIELTIVGLNDPIAALATG"
     gene            complement(2628..4145)
                     /locus_tag="VN478_04890"
     CDS             complement(2628..4145)
                     /locus_tag="VN478_04890"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HWQ22041.1"
                     /translation="MNHEKALLHLSFMSVATLLVFWTARATIPLWVVLPALAVLAAGP
                     WLQRHKLAWEAIIGVALIGILATNGQRLAMDYAARYREFFFLLVPMLFYAGIVRTRLK
                     VPGEPVYLGNIVWLALLITLDVEGAAISIAVLSFLVLLALLLAGTEDDRPRFLQRLVP
                     VAIILVVVTVLATSLPVETGPFSASTAQGLQDFLFGHSTAVDFVPVRHTTNWWKPWPT
                     TGTSLLGSWLMRVAFAEESHVLSRSLLLAPLAILIAWIFLGRVLQQGPGKSLKRLLPA
                     LVVFLCMGGVFVALISIHSKALDTLMFGPLSPWIIDGKLLVPRTWQMFLALRLKVSLF
                     PTSAVILLRLILRNLSILVVLYCGATCIRQAFATRWDRLEGIGRRRDRIIIERTIKRI
                     RSLDDDQLLRDPRGTVIAIFYMAANALYPLDLAMVRGETPTELTTRVARWYPDLAEEV
                     DVLGRLFYVARYSKVEVTPDQVKLAKTTYQTLLEMLKVEARHPRIKTEGALPISQ"
     gene            complement(4147..5388)
                     /locus_tag="VN478_04895"
     CDS             complement(4147..5388)
                     /locus_tag="VN478_04895"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HWQ22042.1"
                     /translation="MRRSVRVAGRAILISLIAIVAWLILGTRPLFYLAALITTYTVVM
                     APLVLRTTDRIRVVKHLRQTVVQMKSQDTLTVSLEGITAHSPLSVQLQPLLPEYIISE
                     SSSWRDRTYALQFHVNRRGIYDLGEGILTVTDPLRLFRLSRRLMADEKLTVFPTVLPL
                     EKLRISLTSPLDGQRVKFAPNYDVSQLAGVRPYESDPMNRIHWKMSAHKGELMVKEFA
                     PSASKTVVVLLNLTLRREPSFTLETFEDYMTMVGASVLQYSYDHKLPFGLTVLGEETL
                     SSSGFSKDATHLFSCYRLLAHAQARMDRDPSSTVFLDYLKRERLRIPARSQLFLIQHE
                     LKEEEIGALLRMQAQFSRISICLFPYGSFLLYGERAVPYFMPDIEQIARLKKTQKILK
                     DASVDLSIIGLNDTLAALGEA"
     gene            5637..6476
                     /locus_tag="VN478_04900"
     CDS             5637..6476
                     /locus_tag="VN478_04900"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HWQ22043.1"
                     /translation="MIQVVMDTTTGMTSQEFEQSGITMVPLYVRDGEKVYREQVDIFP
                     DEFYRRERAGHIYETAQTNPADLVSLFRPIVEAGDEAVCVLISSAISGSVNAARVAAQ
                     TLGAEDKITIVDSLESGYGEAYLGHTAKRMAEAGEKRGEIVRALADIRSRTRTYFIME
                     SLKWLFHGGRLTGAQYFVGSVIKLNPIVWFDEVGRMTSYDKTRTIKVARDHMRQLVAD
                     CGRSGVESATLHWPDNLSEAQEFHAEMEEILQAPVSYTKLSCVLGVHTGPDLLGPCII
                     MKA"
     gene            6604..7470
                     /locus_tag="VN478_04905"
     CDS             6604..7470
                     /locus_tag="VN478_04905"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HWQ22044.1"
                     /translation="MIKVVMDSTCGMTLEDFEKAGIVMAPLYIREGETLYRDGVDMKP
                     AEFYKKEREGVIFESAQTNPDDLVRIFKPILEAGNEIVCVMISGAISGSVNAGHVAAQ
                     TLEAEDRISIVDSRQSGYCQLTMGRLAKSMADAGKSRSEIVEALTELRDRTWVYFIME
                     SLHWLYKGGRLTGAQALIGTIIQLKPIVWFDDAGRMVTHDKIRTLKAGKERLKELVLE
                     MASYGVVSAGLHWADNWDEAKEFGDWMESVLHVPVEYNRVSCVVGCHVGPTVLGPVVV
                     MKEKPTAKNRAD"
     gene            complement(7430..8329)
                     /locus_tag="VN478_04910"
     CDS             complement(7430..8329)
                     /locus_tag="VN478_04910"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HWQ22045.1"
                     /translation="MRRGRTANIASVVASAFVWGTSFAVSKLALRSIPPLWLAWIRFV
                     LASAILLIWLLVRHENLRLDRRALGAMILGGVLGYTLNHIFENVGLALSTASEISLMM
                     GVFPVLSLLVEALIYHKRFSRLAVAGIGLSVIGVVLIVDPGSLGGVLAARRLLGDGLV
                     ILSGICWAFYSILVKNLSLGSSATRTAMFQMLFGSLVLLPLAAALERPVLPVPAAAIW
                     ATIYLAVFCSVGGYSLYNYGVGGMQSTQAVSILNLIPVFGVLTSWVVLHETVTLMQLA
                     GGAVVLVGVLLSLRDSSPSASPS"
     gene            complement(8475..8594)
                     /locus_tag="VN478_04915"
     CDS             complement(8475..8594)
                     /locus_tag="VN478_04915"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWQ22046.1"
                     /translation="MIRKALRESGGNKTKAAQILGISRKSLFNKIRDYKLQDQ"
BASE COUNT         1840 a         2374 c         2813 g         1579 t
ORIGIN      
        1 ggtctctccc cgtcgcagtc gcggccccac ggtcccaact gcctcgacgc ccatcgcaaa
       61 catggcagtt atgctcgtta tcgggtcttc catcagggtt gcatcgttca gggaaaccag
      121 ccgacgcact gcacggatga cctttttgcg ctctgaccgg tccagcatgg acagcatgac
      181 ctcgtgacgg aattcgaaga ccatgtcccg gatgctggcg acaatggaca cgagcaggat
      241 gctggcacct atccagtatg cgaaggcgac gatggtctcg agcgtagcct tcatctcgtt
      301 ctttgtggga ggcaggatgt cgcgttgccg cagtgcttcg acgacgtcgt caatgttctg
      361 cagaggtgct cccagtgcgg tgatccctcc tgagatctga gcggtcttgg tggccgtgta
      421 gatgatggtg aagacgagcg ccgagaccac gatggcggtc gggaagatca gcgcgcgcat
      481 ggtcttgccc cagctattgt gcaggacata gacagagacg atgaccgccg cgatcagtac
      541 ggcgaccatg atggccagcg ggagatacat gaagccgaac aattcccaga agtgctcctc
      601 catgacgcga cgcagcgtga gaccgggaag gttgacgggg attccgcctg ttcccgccgg
      661 cttcggtgac gacgccggcg ttccaggaag catccacacg gcggctctgg gcagtgttgg
      721 cgcttgtacg ataggagagg cgaccgccgc gacaaagacg atcagcagga tgatgaaccc
      781 gggcaggagt cgcctggctg tgcgaggatc ggaggcggtg accgctgaga gcatcgatgc
      841 ggccagcatg atcgccagca cgacagtcgc cacaaggggg tctgtattgc cgaggacggc
      901 atagcagcag gcgacgatgc tgatgcgcag gaaatgcgca ggtgatcccg cggccagcgt
      961 caggttgccc acaagccaga agtagagctc gaacagtacg gtgagcagga ggatggcaag
     1021 aagttcctgc agcgtatgtc ggtctgcagt catcgcaagg tagacaatga tgagacatgt
     1081 ctcgagacca tgatacagcc ggaagagtct ctgattctga tgtggcaagg cgatgttaag
     1141 cacgacgagg gctggtatgc cgaaccacca gggcactgcc agacgggcaa tccagaatgc
     1201 aaggaagcag gcagtattgg cgatgatcag ccgagcgata cgggtcgtgt ccatgatagt
     1261 ctcctcagcc agtggcgagt gcagcgatgg ggtcgttcaa tccgacgatc gtcagttcga
     1321 taccgttctc tctcagcacg tgctgcaagg acctcatacg gagcagcttt gaggtgtctt
     1381 tcaggtagta tggcgccctg tgttcgccgg gcatgaggaa cgtaccttcg gggaagagga
     1441 caatggtgat gcgtgcgaag tgttcacgct gtctgagcag gtgcacgacc tccgtctcgt
     1501 ccatctcgtg ggcgaggagg accagttgtg actgcggcgg gatggcgcgt gcattctggt
     1561 tcagccaatc cagcacgttc gccgtgtctt ccgtctgagc ggcctgggat cgcgccacgg
     1621 ccgtaaggca ggagaggaga tgcatgcgat catggccggt gcccgtccag tacgcacggg
     1681 acccgatcgc tgcgaggcca aagggcaggt tgcggtcatg gacatagtgc aggatactcg
     1741 ctgctgcggt cgacagcgtc tcatccagca cgtcgagggt gaaaccggtg tctctgtgca
     1801 cggtgtagtt gaccagcagg acgactgtct gagatgctga gggggtgaaa tcctttacga
     1861 caagcgtccc cgtgtgagcg ctcatcttcc agtggatccg attcatcggc tcgccgtcgt
     1921 aggggtgagt cccagtgagt tgactggtat ccacgttcgg agcgtaccgg acacgctgac
     1981 cgtcgatggg tgacgtgagc gcgatgtgca tcgctgcaag tgagagcgga cgcgggacga
     2041 cggtcaggac aagcccggat ggtatcagag agtggatact gaagatgcgc aacgggtctt
     2101 gcagggatac ccgtgccgcg cccatggcgt actgtccctt gcgagtggcg tcgaagaaca
     2161 cggttgtgcc atcgtcggca tgagtgctgt ggcgctgaac cacatacggc gggagcagtg
     2221 gttcgacgga cagcagcagt ggctgcgcga cacggtatcc ggaggcggta catgtcagca
     2281 tgtttggctg gcgcactgtc gcgacactgt ggcggaggac gctcgtgacg gcgagccgct
     2341 ggtgctgtga cgtgagcatg ccgcacagga ggaccgtgta tgacagagcg gcaagaccca
     2401 cgatcagaaa ggaagcactg cggagcagac gcgcgacgcc cagcatgagg aaggacgcca
     2461 gcagaccagt gcccgctgtc ctgaagaaca cgcgaaggcc gccgggccgt cccggtgtac
     2521 gggaacgccc ctgtggtagt gccttcatct gatgaacccc tgcagtatcc atcgcgcctc
     2581 actcactaca tcgggcaggg tcgccggcac gttacagacc ggttgggcta ctgcgagatg
     2641 ggcaacgccc cttcagtctt gatacgcggg tgccgggcct cgaccttcag catctccagc
     2701 agcgtctggt aggtggtctt ggccagcttc acctgatcgg gagtcacctc gaccttcgag
     2761 tatcgtgcga catagaagag gcgtcccagg acgtcgacct cctcggcaag gtccggatac
     2821 caccgggcga cgcgagtcgt cagttcagtc ggggtttcgc cgcgcaccat ggccaggtcg
     2881 agaggataga gggcgttggc tgccatgtag aagatcgcaa tgactgttcc gcgcgggtcg
     2941 cgcagcagct ggtcgtcgtc caggctgcgg atcctcttga tggtgcgctc gatgatgatg
     3001 cggtcgcgcc tgcgaccgat cccctcgaga cggtcccacc gcgtcgcaaa ggcttgccgg
     3061 atacaggttg cgccgcagta caggacgacg agaatcgaca ggttgcgcag gatgaggcgg
     3121 aggaggatca ctgcagaagt cgggaagagg ctgaccttca gcctgagtgc caggaacatc
     3181 tgccaggtac gaggcaccag gagcttccca tcgatgatcc agggggacaa gggaccaaac
     3241 atcagagtgt ccagagcctt gctgtgtatg ctgatgaggg ccacgaatac gccacccatg
     3301 cagaggaaca ccacaagagc cggcagaagt ctcttcaggg acttgcccgg gccttgctgc
     3361 aggactcgtc ccaggaagat ccacgctatc aggatggcca gcggagccag cagcagagag
     3421 cgcgagagaa cgtgagactc ctcggcgaaa gcgactctca ttagccagga gccgagaagg
     3481 ctggttcccg tcgtcggcca gggcttccac cagtttgtcg tatggcgcac cgggacgaag
     3541 tcaaccgcgg tggaatgtcc aaacagaaag tcctgaagtc cttgcgcggt cgatgcactg
     3601 aacggaccgg tttcgaccgg cagggacgtc gccaggacgg taaccacgac caggatgatg
     3661 gcgacgggta ccagtcgctg caggaaccgc ggtcggtcgt cttccgttcc ggcaagcagc
     3721 aacgccagca ggacgagaaa cgatagtacg gcaatcgaga tggctgctcc ttcgacatcc
     3781 agcgtgatga gcagtgccag ccagacgatg ttgccgagat agacgggttc gccgggaacc
     3841 ttcagcctcg tgcggacgat gccggcgtag aagagcattg gcacgaggag gaagaagaat
     3901 tcgcgatagc gcgcggcata gtccatggcc agacgctggc cgttcgtagc cagaataccg
     3961 atcaaagcaa cgccgataat ggcttcccac gcaagcttgt gtcgctgcag ccatggtcct
     4021 gctgccagga cggcaagagc gggcagtacg acccacagag ggatggtggc gcgtgccgtc
     4081 cagaagacca ggagtgtcgc cactgacatg aatgagagat gaagcagcgc cttctcgtgg
     4141 ttcatttcaa gcctctccca gcgcagccag cgtgtcattc agtccgataa tggacaggtc
     4201 cacactggca tccttcagga tcttttgcgt cttcttgagc cgagcgatct gttcgatgtc
     4261 cggcataaag tacgggaccg cccgctcacc gtagagcagg aagcttccat acgggaacag
     4321 gcagatcgag atgcgagaga actgtgcctg catgcgaagc agtgctccga tctcctcctc
     4381 cttcagctcg tgctggatga ggaagagctg tgaccgggct gggatgcgga gccgctcccg
     4441 tttcagatag tcgaggaaca ccgtgctgga agggtcccgg tccatccgtg cctgcgcgtg
     4501 cgcgagcagg cgatagcagg agaacaggtg cgtggcgtcc ttggagaagc cgctggatga
     4561 gagcgtttcc tcccccagga cggtcagccc gaaaggaagc ttgtggtcat aggaatactg
     4621 gaggacgctg gcgccaacca tggtcatata gtcttcgaag gtctccaggg tgaagctggg
     4681 ctcgcggcgg agggtcaggt tgagcaggac gacgaccgtc ttcgaggcgg acggggcgaa
     4741 ctccttcacc atgagttcgc ccttgtgagc actcatcttc cagtggatcc ggttcatggg
     4801 atcgctctcg tagggccgca cgccggcaag ctgtgagacg tcgtagttgg gcgcgaactt
     4861 gacgcgctgc ccatcgaggg gagacgtcag cgatatgcgc agcttctcaa ggggaaggac
     4921 tgtggggaac acggtgagtt tctcatccgc catcaggcgc ctgctgaggc gaaagagtcg
     4981 gagaggatcg gtcacggtca ggataccttc cccgaggtca tatatccctc gacggttgac
     5041 gtgaaattgc agggcgtacg tccggtcgcg ccaggaggaa ctctcgctga tgatgtactc
     5101 gggcagaaga ggctggagct gaacggagag aggagagtgt gctgtgatcc cctcaagcga
     5161 gaccgtcagt gtgtcctgag acttcatctg gacgacggtc tgtcgcaggt gcttgacgac
     5221 cctgattcgg tcggtagtcc tcagcacaag cggagccatg accaccgtat acgttgtaat
     5281 gagagctgca aggtagaaca gggggcgggt gccgaggatg agccatgcca ctatcgcaat
     5341 caatgatatg aggattgcgc gtcctgcgac gcggacggat cgcctcatcg cgccatcacg
     5401 accctggact ctgcctcatg cgttctgccc gttgccacat gtcacgtcca cctctctctt
     5461 agctggctgc cttttgcaga ctacggctat tgtacggcgt cggggctgat ttgcaatgct
     5521 ggagcggtgc aagaggttgc caaggactct tcgggggaag cccccaagtt cagctggttg
     5581 tcgcgggcaa ccttttctgg tagactactc gggcaaactg tggaggtgaa cgacacatga
     5641 tccaggttgt catggatacg acgacgggaa tgaccagtca ggagttcgaa cagtccggca
     5701 taacgatggt tcctctctac gttcgggacg gagagaaggt ctatcgggaa caggtggaca
     5761 tcttccctga cgagttctat cggcgggaga gggctggtca catctacgag actgctcaga
     5821 cgaatcctgc tgacctggtc tcactcttca ggcctatcgt cgaggcgggc gacgaagctg
     5881 tgtgcgtcct tatctcaagt gccatctcgg gatccgtcaa tgcggcccgc gtcgccgccc
     5941 agacccttgg ggccgaagac aagatcacta tcgtggactc cctcgaatcg ggctacggtg
     6001 aggcctatct tggccacacc gcgaagagaa tggctgaagc cggcgagaaa cgtggcgaga
     6061 tcgttcgggc ccttgccgac attcgcagcc ggacacgcac gtacttcatc atggagtcgc
     6121 tcaagtggct gttccatgga ggtcgcctga cgggagcaca gtacttcgtc ggttcggtga
     6181 tcaagttgaa cccgatcgtc tggtttgacg aggtcgggcg catgacgtca tatgacaaga
     6241 cacggacgat caaggttgcc agagatcaca tgcgtcagct ggtcgccgat tgtggcagga
     6301 gtggcgtcga atcagcgact ctgcattggc cggacaacct gagcgaggct caggagtttc
     6361 atgccgagat ggaggagatc ctccaggccc ctgtgtccta tacgaagctg tcgtgtgtgc
     6421 tgggtgtgca cacgggcccc gaccttcttg gcccatgtat catcatgaag gcatgaccac
     6481 agcatccagc caaaccagcc ggcgacgcgt atcgtcatgc tttgctgcag gacgatgggc
     6541 gcgtctgcaa gtgacaccgg aacacggtga aaggtggccg aagggccccc gggaaggagt
     6601 cgaatgatca aagtagtcat ggattcgacg tgcggcatga cgctcgagga tttcgagaaa
     6661 gcaggcatag taatggcgcc tctctatatc cgcgaaggag agactctcta tcgcgatggc
     6721 gtggacatga aacccgccga attctacaag aaggagcgtg agggcgtcat cttcgagagc
     6781 gcccagacca accccgacga cctggtacgt attttcaagc cgatcctgga ggctggaaac
     6841 gagatcgtct gtgtcatgat atcgggggcg atctcggggt ccgtcaacgc tgggcatgtt
     6901 gctgcccaga cgctggaggc ggaggaccgc atctcgatcg tggactctcg ccagagtggc
     6961 tactgccagc tcacgatggg cagactggca aagagcatgg ctgacgcggg caagagccgc
     7021 tcggagatcg tcgaggcgct cacggagctg cgagatcgca catgggtcta cttcatcatg
     7081 gaatcgctgc actggctcta caagggcggg cgtctcaccg gtgcgcaggc tctcatcggg
     7141 acgatcatcc agctcaagcc aatcgtctgg ttcgacgatg ccggccgcat ggtgacgcat
     7201 gacaagatcc gaacactgaa ggccggcaag gaacggctca aggaactggt cctggagatg
     7261 gcatcctacg gcgtcgtgtc tgcaggactg cactgggccg acaactggga tgaggccaaa
     7321 gagtttggcg actggatgga atcggtgctc cacgtgccgg tcgagtacaa cagagtctca
     7381 tgcgtggtcg gctgtcatgt gggtccgacg gttctgggac ctgtggtcgt catgaaggag
     7441 aagccgacgg cgaagaatcg cgcagactga gcaggacgcc cacaaggacg accgctccgc
     7501 cggccagctg catcagagtc acagtctcgt gcagaacgac ccaggaagtc agtacgccga
     7561 aaaccgggat gaggttgagg atactgaccg cctgggtcga ttgcatccct cccacgccat
     7621 agttgtagag tgagtacccc ccgaccgaac agaagacggc aaggtagatg gtggcccaaa
     7681 tggccgccgc ggggaccggg aggactggcc gctcgagagc agccgccaga ggaagcagga
     7741 ccagactgcc gaacagcatc tgaaacatcg cagtcctggt ggccgagctg cccagggaca
     7801 agttcttgac gaggatgctg tagaatgccc agcagatgcc tgacaggatg accaggccgt
     7861 cgcccagcag gcgcctggct gccaggaccc ctccgagaga acccgggtcg acgatgagga
     7921 cgacgccgat gacagacagc ccgatgccgg ctacagcaag gcgtgagaac ctcttgtggt
     7981 agatcagcgc ctcgaccagg agagacagca cgggaaagac ccccatcatg agcgagatct
     8041 cggaggctgt cgagagggca agtccgacgt tctcgaagat gtggttcagc gtatagccca
     8101 ggactcctcc aaggatcatg gctccgaggg cccggcggtc caggcggagg ttttcatgac
     8161 gtacgagcag ccagatgagc aggatggcac tggcaagcac gaagcgtatc cacgcgagcc
     8221 agagaggtgg aatcgagcgc agagccagct tggagacggc aaaggacgtt ccccagacga
     8281 atgccgacgc gacgacggac gctatgttgg ctgttcggcc tctacgcatg gttgcatgat
     8341 ggtacccctt gccagcgctc ttgtcaaaga gatcaaagcg aaacggtgcc ggcgcgcgct
     8401 catgaaacga agaagccccg gccgtctggc gtccgggact cacgacatcg tgagatcagt
     8461 tgcagagaga ctgcctactg atcctgcagt ttgtagtccc tgatcttgtt gaacagcgac
     8521 ttgcgggaga tgccgaggat ctgggcggcc ttggtcttgt tgccgccgga ttcccgcagc
     8581 gccttgcgga tcatgtcgac tgtctc
//
LOCUS       DATMZD010000236         4078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_147117,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000236 DATMZD010000000
VERSION     DATMZD010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4078
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147117"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="VN478_04920"
     CDS             complement(<1..449)
                     /locus_tag="VN478_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HWQ22047.1"
                     /translation="MNEEQRPNPDTLLHHLQQEEADQKSYDQGHLKIYLGYVAGVGKT
                     YGMLEEAINLKDQGIDVVIGLVETHGRAETEALVRDLEIIPRRAVVLGGLKLDDLDLD
                     AVLARKPTVVLVDELAHTNIPGSRHEKRFQDVKELLDAGISVLTTLN"
     gene            complement(451..1023)
                     /gene="kdpC"
                     /locus_tag="VN478_04925"
     CDS             complement(451..1023)
                     /gene="kdpC"
                     /locus_tag="VN478_04925"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HWQ22048.1"
                     /translation="MKELKRALIAFGILVVITGIIYPLAVTGIAQLTMFHRANGSLVS
                     VSGHVVGSELIGQQWTSSKYFHGRPSAVQYDASGSGGTNLGPSSADLEKTVIERVAKV
                     RSENGLAADATVPADLVTASASGLDPDISPESALLQVRRVAAARGLAPSAVEHLVTVN
                     TRKPFLGIWGQSRVNVLELNIALDKLSETH"
     gene            complement(1035..3068)
                     /gene="kdpB"
                     /locus_tag="VN478_04930"
     CDS             complement(1035..3068)
                     /gene="kdpB"
                     /locus_tag="VN478_04930"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HWQ22049.1"
                     /translation="MRKKGMLDSSVTRKAIRDAVKKLNPLTLWHNPVMLVVEIGSIIT
                     TANTVFSAFQHTGFWFNFQISVWLWATVLFANFAEALAEGRGKAQADSLKKTRNEAVA
                     NRLRPDGATETVAAIDLIKGDIVIVTDNETIPSDGEIVEGVALVDESAVTGESAPVIR
                     ESGGDRTGVTGGTHVLSGTIRLRITANMGDTFLDQMIAMVESSSRRKTPNEIALEILL
                     VGLTALFLVVTVTLQPFAGYMGVTIQIPVLVALLVCLMPTTIGGLLPAIGIAGMDRLI
                     EHNVIALSGRAVEASGDVSVVLLDKTGTITLGNRMATEFLPAESVTEHQLMESSVLAS
                     LADDTPEGRSIVTLAKQRLGIRGRDIRAPQGSMFLPFSADTRMSGIDYADRQIRKGAV
                     DAIKQWVEENGGQFPDTVHALVDGVARQGETPLVVSDGAVVMGIIRLKDILKQDIKER
                     LGQLHAFGIKSVMITGDNPLTAAAIAAEAGIDDFVAQARPETKLNLIRQYQEDGNMVA
                     MIGDGTNDAPALAQADVGVAMNAGTQSAREAANLVDLDSNPTKLLDIVEIGKQILITR
                     GSLTTFSIANDVAKYFAIIPAIFAAKYPQLAILNVMHLHSPASAILSAVVFNALIIPS
                     LIPLAMRGVQYHQSSANALLWRNLTIYGAGGLALPFVGIKLIDLFVVAIRLAR"
     gene            complement(3085..>4078)
                     /gene="kdpA"
                     /locus_tag="VN478_04935"
     CDS             complement(3085..>4078)
                     /gene="kdpA"
                     /locus_tag="VN478_04935"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HWQ22050.1"
                     /translation="GTNGGGFFNANAAHPFESPNPFTDLLLVFSLLLIPAALPFTFGE
                     MLGDRKQGTALFLAMLIMFSIGLGISLHYELAGNPALARAGVAGGVNMEGKEQRFGPI
                     ISTLFAQSTTVTSTGAVDSMHDSFTPIPGLIMVFNMTVGEVIFGGVGVGLVGMFLYVI
                     LTLFIVGLMIGRTPEYLGKKLEPREMLMAVVGLLGPPIAMLLMAGLAFSSPSAIASVG
                     NAGSHGMTETIYACASAAGNNGSAFAGLDANTPFYNIGLALLMLVGRFATILPALAIA
                     GSVAGKKRVQSGVTFSTTSILFVFMLIFVVVIVGGLTYFSPLVLGPLLEFLKLR"
BASE COUNT          960 a         1083 c         1118 g          917 t
ORIGIN      
        1 atgttgaggg tcgtaagaac actgatgcct gcatccagca gttccttcac atcctggaac
       61 cttttctcat gccggcttcc cggaatgttt gtgtgggcaa gttcatctac cagaacgacg
      121 gttggtttgc gtgcaaggac agcgtcgaga tccagatcat cgagtttgag accaccaagg
      181 acgacggcgc gtcgtgggat aatctccaga tccctgacaa gcgcctcggt ttctgctcgc
      241 ccatgggttt cgacaagacc gataacaaca tctatcccct ggtccttgag gtttatcgct
      301 tcttccagca ttccatacgt cttgccgacg ccggcgacgt agccgaggta tatcttcagg
      361 tgaccttggt cgtaggattt ctgatctgcc tcttcctgtt gcagatgatg cagaagtgtg
      421 tctgggttcg gccgttgttc ctcgttcatt tcagtgtgtc tcggagagct tatccagggc
      481 aatgttgagt tcgaggacat tcacgcgcga ttgtccccag ataccgagga agggcttcct
      541 ggtattgacc gtgacaagat gttcgactgc actcggtgca aggccgcgtg cagcggcgac
      601 acgtcgcact tgcagcagag cggactcggg actgatgtca ggatccagac cgcttgccga
      661 ggccgttacg agatcagcag gaacggttgc atccgcagcc agaccgttct cggagcgaac
      721 cttggcaact cgttctatga cggtcttctc aaggtcagcg cttgagggtc cgagattcgt
      781 tccaccagag cctgaagcgt catattgcac ggctgacggg cggccatgga agtacttcga
      841 tgaagtccat tgctggccta tgagttcaga gccgacgaca tgtccgctta ctgagacaag
      901 acttccatta gcacggtgga acatcgtcag ctgtgcaata cctgtcacag cgagtggata
      961 gatgattccc gtaatgacaa caaggattcc aaaagcgatc agtgctctct tcagttcttt
     1021 catgtcgaat ctccttaccg tgcaagcctg atggcgacga caaacagatc aatgagtttg
     1081 atgcccacaa atggcagtgc cagtccaccg gcaccataga tggtcaaatt ccgccagagg
     1141 agtgcgtttg cgctggattg gtgatactgg actcctcgca tggcaagtgg aatgagtgat
     1201 ggaataatga gagcattgaa cacgacggcc gacagaatag cgctggctgg tgaatgcagg
     1261 tgcatgacat tgagaatggc cagttgtgga tactttgcag caaaaatcgc cggaattatg
     1321 gcaaagtact tggcaacatc gttcgcgatg ctgaaggtgg tcaatgagcc ccgtgtaatc
     1381 aggatctgtt tgccgatctc gacaatgtcc aacaacttcg tcggattcga atcaagatca
     1441 acaagatttg ctgcttcccg tgcagattgt gtgcctgcat tcatcgcaac gcctacgtca
     1501 gcctgtgcaa gcgccggcgc atcgttggtt ccatctccga tcatggcgac catattaccg
     1561 tcttcctggt actgtcggat gaggttgagt ttcgtctcgg gtcgtgcttg tgccacgaag
     1621 tcatcgatgc ctgcttcagc cgctatggct gcagcggtca aggggttgtc acccgtaatc
     1681 atgaccgatt tgataccaaa cgcgtgaagc tggcccaatc gttccttgat atcctgtttg
     1741 aggatatcct tcaggcgaat gatgcccatg acgacagcgc catctgagac gacgaggggt
     1801 gtctcaccct ggcgggctac accgtcgacc aaggcatgaa ctgtgtccgg gaattgcccc
     1861 ccgttctcct cgacccattg tttgatcgcg tctacggcac ctttgcggat ctgccggtct
     1921 gcgtagtcaa taccgctcat ccgcgtgtcg gcactgaacg ggaggaacat cgatccctgc
     1981 ggggcgcgga tatcgcgtcc acgtatgcca agcctctgct tggccagggt gacgatgcta
     2041 cgaccttcag gcgtgtcatc tgccagtgac gcaagtactg aactctccat cagctggtgc
     2101 tccgtgacgc tctcggcagg aagaaactcc gttgccatgc ggtttcccag cgtaatcgtg
     2161 ccggtcttgt ccagcagaac gactgaaacg tcgcctgatg cttccacggc tcgaccggat
     2221 agcgcgatga cgttgtgttc gatgagccgg tccattcctg cgataccgat tgcgggcaac
     2281 aggccgccaa tagttgtcgg catgaggcag accagaagcg ctaccagcac aggaatctga
     2341 atagttacgc ccatgtatcc ggcgaatggc tgcaacgtaa ctgttacaac gaggaacagt
     2401 gctgtgagtc cgaccaggag aatctccaga gctatctcat ttggcgtttt gcgccgcgag
     2461 gagctctcga ccatcgcgat catctggtcc aggaacgtat cacccatatt ggcggtgatg
     2521 cgcaggcgaa tagtccccga caatacgtgg gtcccgccgg taacaccagt gcggtctcct
     2581 cctgactcac ggatgacagg cgctgattcg ccggtcacag cggattcatc cactaatgct
     2641 acgccttcga caatctcgcc atcgctgggg atggtctcgt tgtcggtgac aatgactata
     2701 tctcccttga tgagatcaat agctgccacg gtctctgtgg ctccatctgg acgaagacgg
     2761 ttagcaactg cttcgttgcg agtcttcttg agtgaatcag cctgcgcctt tccacgtcct
     2821 tcggcgagcg cctcggcgaa attggcaaac aggacagtag cccaaagcca cacggaaatc
     2881 tgaaaattga accagaaacc tgtgtgctga aaagcgctga acacggtatt ggcggttgtg
     2941 ataattgagc cgatctctac gaccagcata actggattgt gccacagagt aaggggattg
     3001 agtttcttga ccgcgtcgcg aatagctttg cgtgtcacag atgagtcgag catacccttc
     3061 tttctcatgt attcctccac gatcctagcg gagtttcagg aactcaagca gtggcccgag
     3121 aaccagcggc gagaaatagg tcagtccccc cacaataaca acaacaaaaa tcagcatgaa
     3181 cacgaacaag atgctggttg tcgaaaacgt tacgcctgat tggactctct tctttcctgc
     3241 taccgaccca gcaatggcaa gagcaggaag aatggtggcg aaacggccga caagcatgag
     3301 cagtgcaagc ccaatgttgt agaagggtgt gtttgcatcg agtccggcaa aagctgaacc
     3361 gttattcccg gctgcagatg cacatgcata gatagtctcg gtcattccgt gagagccggc
     3421 gtttccaaca gaagcaatgg cagagggact agagaaagca agaccagcca taagaagcat
     3481 ggcgatcgga gggccaagaa gcccgacaac tgccatgagc atttcgcgtg gttcaagctt
     3541 cttccccaga tactcgggag tacgaccaat catgagtccc acgataaaca gtgtcaagat
     3601 gacatacaga aacatcccga ctaaaccgac accgactccg ccgaagatga cttctccaac
     3661 agtcatattg aaaaccatga tcagaccagg gataggagtg aaactgtcgt gcatggagtc
     3721 gacagcgccg gtggatgtca ccgtcgtcga ctgagcgaag agagtcgaga tgatcgggcc
     3781 gaatcgctgc tccttgccct ccatgttgac tccccctgcg acgccggctc gtgcaagtgc
     3841 gggattgcct gcgagttcat agtgaagcga aatccccaaa ccgatgctga acatgatcag
     3901 catggccagg aacagagcgg taccttgttt gcgatcgcca agcatttcac caaatgtgaa
     3961 cggcaaagcg gctggtatga gaagaagaga gaagacaaga aggagatccg taaagggatt
     4021 tggactttcg aaggggtgag ctgcgttggc gttgaagaat ccgcctccgt tggtgccg
//
LOCUS       DATMZD010000237         6576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_17018,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000237 DATMZD010000000
VERSION     DATMZD010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6576
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17018"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="VN478_04940"
     CDS             complement(<1..765)
                     /locus_tag="VN478_04940"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HWQ22051.1"
                     /translation="MNRLAVFDLDGTLLDEQSSIPVQTAERIRAAGDPGLACTVASGR
                     DLDRVSPFLRQLGWLEIPAITEQGAVVLEPDNGHILMERGISREVTLNTVDVVRSIHI
                     PLNIVLYGRDAPQVFRSAGAPSFVDGWDGGWYSSQLRDVPDVREILTGGVRKISVKCL
                     SEHTDVIRQRLADSLGGCANVVKADVNFVNVMDVGVNKGTALLWLMDYLGMPMTSVMV
                     VGDCEADRSMFTVAGIPVAVANADEQTRRMAKYIVPS"
     gene            complement(818..2305)
                     /locus_tag="VN478_04945"
     CDS             complement(818..2305)
                     /locus_tag="VN478_04945"
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HWQ22052.1"
                     /translation="MLYGFGFWSRKVLWSVIALMFLCVPSLTARAQTTTVVLTTGSAR
                     AVVDGRSVTMLLPPVTDQSSGQVLVPVGFLARQFGFGVSPVAQSTAITLSRVGRTVCV
                     TPGSLECLVDGYVVRLSVAPRGFLGEVLVAGADVQLLLSVQYAHGSTDRESVYTRVTE
                     DPVVEGPVDPDAVTLQTRHEQVFMQTVDFSIVRIDMAAKGIRVLSCQSSGGLGTGRYP
                     SAYVDSLKPLALMNATPFNLVSKLMSGSVQDQGTPVYYSGTYISTIGIDADNTPFYVE
                     GTARAVVRLDTGKEIPIARINQLPAEPSSVSLSLYSNYYRGGLSVAASEALSVLEEGR
                     VVRLLSGGYFTPRTLAPGQMAVYARNPGVVAALRASAGVDVRSYVGTRDCTGSTFVQC
                     GPVVVRGSKPYIDYRKYKDISRTSKLGSRAFVGMDDQKHLYFIVTPAHVRLQFGDVSL
                     ALARLGLFTNVVTLDGGSSTTLYYKGQYILKGTRALTNVLCVPST"
     gene            2512..5421
                     /locus_tag="VN478_04950"
     CDS             2512..5421
                     /locus_tag="VN478_04950"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HWQ22053.1"
                     /translation="MDNDRSLRRGTVGMRRALRSAAALAVIAVLLPGRAPKVCMAAPL
                     NAPPQLVVVANVYEPFAFPRDGRLVGFDVDLVGLIASLNGWDVRYEETTFVDELQRIE
                     DGTADLGIGSIFRTPGRASRFAFTDSYLSSGLVLVTPVESSVHSPRDLTGRRVAVKAR
                     TASDDYVASLCNRYGQPLVQRYMSSEGVFAALAGGQADAAVHDYINAQFLIGDRFQGS
                     LVIQKGSLFGGFLNGRRPVAYPAARNMLPLLRQFNTTLTEIRRSGMLAKLETKWFGTT
                     VSYYANSDLLRVTLLVSAGIAAVLLVIYLYVSRRRATRTARDRAAYYQQLFAAVPEPA
                     LLVDGAEGQLRIEAANGALCTLLAREESKLLGATLGSIMFMEQSDVSMTLTSLLDRSL
                     PSTRWQLVASDRTLIPVEVRMSSLQYPSKRVLIVARDLRDQLRAEQTIHAAYQQYHTL
                     FDEAPDPILIISDHVITQASAAAGRVLGATIEQLTGHAIGEMSPAVQPDGRPSSEAIR
                     SFEDLAFSGQPQRFDWTFVTHDGRRVTCEASLQSIPSAGKAIVQGIFRDVTEQRLAED
                     RARQLEKELLQSQKMEAVGRLAGGIAHDFNNIMGGIMGHASLLRVDATEGTDLYQGLD
                     TIERAARRAADLTRRLLSFSRNSTPTVTDVDVGGVIADTLSIAMPGFDKRTTLVQNVS
                     SDLAYVPGDRTQLEEVFLNLIINARDAMGHHDGTLTVDADNIALDAPFCISHGVPALP
                     DGDYVQVRVSDTGCGVSPEAREHMFEPFFTTRPEGTGLGLSIVWRVVHDHGGTVVVTS
                     VPDRGTTFTLYLPAVSRAEAQGRARQTPVSGSLPRSVRGEAILIVDDEDVVRSVAVKV
                     LSGLGYRVIDTADPMNALDIYADSWQSIDLVLIDMMMPHMNGKDLFEKLRQINPAVAA
                     ILMSGYDATDTPFASMGFVAFVPKPYTLQELATRVRQSLSTPAARASSPSEEPTA"
     gene            complement(5361..>6576)
                     /locus_tag="VN478_04955"
     CDS             complement(5361..>6576)
                     /locus_tag="VN478_04955"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWQ22054.1"
                     /translation="SWLVLKLTNSPLKLGVLTSIQFLPSLLLSVFIGPIIDRYPKRSI
                     LLFTQIMYAVAAGALAAVAFGGHAQYWHVLVIAAVTGLISAVDWPTRQAFVGEQVNDR
                     SAVVNAVALNSTMFNIARVIGPGIGGVLIAAVGIPWTFALNSVSFIAVIISLLIMRAG
                     RKPTKSSKGNYVQEVREGGRYIARNQVVMSLLIIAGTISLFLLNFNILIPSFAKLTLG
                     LQSDGYGGLMSAMGAGALAAGVLMSLGGARLEPTPAYVYGSGFILAVAMILVGIQRNF
                     YLTALLLVFCGFGMAALSTMCNTALQMQSPDNMRGRVMAAYNFVFVGSTPIGALYAGK
                     ISDALGASMGFLVSGLIGIVFLGAMLVLVTPRSFRGLRTFAVADSSAERGSQAVGSSD
                     GLDARAAGVDKD"
BASE COUNT         1357 a         2083 c         1898 g         1238 t
ORIGIN      
        1 ggacggcacg atgtacttcg ccatccgccg cgtctgttca tcagcgttcg ccaccgcgac
       61 cgggattccg gcgactgtga acatgctccg gtccgcctcg caatccccta ccaccatcac
      121 actggtcatc ggcatcccca ggtaatccat cagccagaga agcgcggtcc ccttgttcac
      181 gccgacgtcc atgacgttga cgaaattgac gtccgccttg acgacgttcg cgcaaccccc
      241 gaggctgtcc gccagacgtt gtctgatcac gtcggtatgt tccgaaagac atttgacaga
      301 tatcttccgg actcccccgg tcaggatctc cctcacatca ggcacatcac gcaactggga
      361 cgagtaccag ccaccatccc atccgtcgac aaagcttggc gccccagcgc ttcggaatac
      421 ctgtggcgcg tcccgaccgt acaggacgat attcagggga atgtgtatcg aacggaccac
      481 gtccactgta ttgagggtga cctctcgcga gatgccgcgt tccatcagga tgtggccatt
      541 gtcaggctcc agaacgactg caccctgctc ggtgatagcc ggaatctcga gccatccgag
      601 ctgccggaga aatggcgaca cgcgatcgag atcacggccc gaggcaacag tacacgcgag
      661 tccagggtct cccgcggccc ggatacgctc ggcggtctgc acagggatcg aggactgctc
      721 atcgaggagc gttccatcaa ggtcaaagac ggcaagtctg ttcatgtacc cacatccctc
      781 atgcacctgc ccgttcgcag actgcaggat cctggtacta cgtcgacgga acacacagca
      841 cgttggtcag tgcgcgtgtt cccttcagga tgtactgacc tttgtagtag agtgtcgtcg
      901 aactcccccc gtcaagtgtc acgacgttag tgaacaagcc gaggcgagcc agtgcgaggc
      961 tcacatctcc gaactgaaga cgcacatgcg ccggcgtcac gatgaagtat agatgcttct
     1021 gatcatccat gccaacaaat gcgcgggaac ccagcttcga cgtccgactg atgtccttgt
     1081 acttgcgata gtctatgtac ggcttgcttc cccgcacgac gaccggcccg cactgtacga
     1141 acgtggagcc tgtgcagtca cgcgttccaa cataggaacg gacatccact ccagccgacg
     1201 ctcggagagc tgccaccacc cccgggtttc gtgcgtacac tgccatttgc cctggagcca
     1261 gcgtccgcgg tgtgaagtat ccgccggaca agagccggac aactctcccc tcctcaagta
     1321 ccgagagcgc ctcgctcgca gcgacggaga gaccacctcg gtagtagttc gagtacaggg
     1381 agagtgacac tgacgatggt tcggcgggca gctggttgat gcgggcgatc gggatctcct
     1441 tgcctgtgtc caggcgcaca actgcgcggg ccgtgccttc cacgtagaat ggcgtgttgt
     1501 ccgcgtcgat gccgatcgtc gagatatatg ttcccgagta gtaaacgggc gttccctggt
     1561 cctggaccga tccgctcatg agtttgctca ccaagttgaa gggtgtcgcg ttcatgagcg
     1621 ccagtggttt cagactgtcc acatatgcgg atgggtagcg accggtcccc agaccaccag
     1681 aggactgaca gctcaggacc cggatcccct tggccgccat gtcgatacgt acgatactga
     1741 agtctaccgt ctgcatgaat acctgttcat ggcgagtctg aagggtgacg gcgtcaggat
     1801 cgacggggcc ctccacaacc ggatcctcgg taacccttgt gtacaccgac tcccggtcgg
     1861 tcgagccatg ggcatactgc actgagagca gcagctgcac gtccgcgccg gctaccagca
     1921 cttcaccaag gaagcctcgc ggagccaccg agagtctgac gacataacca tccacaagac
     1981 actcgagact gcctggagtc acgcatacag tgcgcccgac tctcgagagc gtgattgccg
     2041 tactctgagc cacaggcgac acgccaaaac caaactgtct cgccagaaac ccgaccggga
     2101 ccagtacctg tccggaggac tgatctgtga cggggggaag gagcatggtc acgctgcgcc
     2161 cgtccacgac ggcccgcgca ctgcctgtcg tgaggacgac cgtcgtggtc tgggcacgcg
     2221 ccgtcagcga cgggacacac aagaacatta atgctatgac actccacagc acttttcgag
     2281 accagaatcc aaatccgtac agcatttcga accacctcct gcgagaacag tatacgcgca
     2341 tatcttagac gacagacact ctcgtgacgt tccctgtcgc ccgtcctcct ggtggggacc
     2401 gcgaaccatg cggaggacgg ctgcagatga tagatgccgg ctttacagga gggacttacg
     2461 ggatatcatg gacacatgtt gacagcatgt cgagccggga gggagacctc ggtggacaat
     2521 gatcgttcat tgcgccgggg gacggtcgga atgcgacgtg ccctgcggtc tgcggccgcc
     2581 cttgccgtca tcgccgtgct ccttccggga cgagcgccga aggtctgcat ggcagctcca
     2641 ctcaatgcac ctcctcagct ggtggtcgtc gcaaacgtct acgagccctt cgcgtttccg
     2701 cgcgacggac gcctggtcgg attcgatgtc gatctagtgg gtctgatcgc ctccctcaac
     2761 gggtgggatg tccgatacga agagacgacc ttcgtcgacg aactgcagcg aatcgaggac
     2821 ggcacagcgg atttgggaat cgggtcgatc ttccggactc ctggccgcgc ttcccgtttt
     2881 gccttcaccg actcatacct gagttcgggc cttgtcctcg tcaccccggt ggaaagcagc
     2941 gtccactccc cacgtgatct caccggccgc cgggttgccg tcaaggcacg gacagcctcc
     3001 gacgactacg tcgccagcct gtgcaatcga tacgggcagc ccctggtaca gcgctacatg
     3061 tcgtctgagg gcgtgttcgc tgcactcgcc ggaggtcaag ccgacgcagc cgttcacgac
     3121 tacatcaatg cccagtttct catcggcgac cgtttccagg gaagccttgt gatccagaag
     3181 ggcagtctgt tcggcggttt cctcaacgga cgccgtcccg tcgcgtaccc ggcagctcgg
     3241 aacatgcttc cattgctgcg tcagttcaac acgacactga cggagatccg tcgcagcggg
     3301 atgctggcga aactggagac caagtggttt ggcaccacag tctcctacta cgccaacagt
     3361 gacctgttgc gagtcacact gctggtctct gcaggcatcg cggccgttct gctggtcatc
     3421 tatctgtacg tctcgcgccg acgcgcgaca cgtaccgccc gcgatcgggc cgcctactac
     3481 cagcagctgt tcgcggccgt ccctgaaccc gcgctgcttg tggacggtgc tgagggtcag
     3541 ctgcggatcg aagcggccaa cggagccctg tgcaccctgc ttgctcgaga agagtccaaa
     3601 ctccttggcg ccacgcttgg gtccatcatg ttcatggaac agtcggacgt gtcgatgaca
     3661 ctgacgtctc tgctggaccg aagtctcccc tcgacacgct ggcagctggt ggcgtcggac
     3721 aggactctca ttcctgtcga agtcaggatg tcgagcctgc agtatccgag caaacgagta
     3781 ctcatcgtcg cccgcgatct ccgggaccag ctcagagctg aacagaccat ccacgcagcg
     3841 tatcagcagt atcacaccct gttcgacgag gctcccgatc ctatcctgat catcagcgac
     3901 cacgtcatca cgcaggcaag cgccgcagcc ggacgtgtgc tcggcgccac gatcgagcag
     3961 ctgacggggc atgccattgg ggagatgagc cctgcggtgc agccagatgg tcgtccgtcc
     4021 tccgaagcca tccgctcatt cgaggacctt gccttctcgg gccagcctca gcggtttgac
     4081 tggaccttcg tcactcatga cggcaggcga gtcacatgcg aggcctccct gcagtccata
     4141 ccatcagccg ggaaggccat cgtccagggg atctttcgtg acgtcacaga gcagcggctc
     4201 gcggaagatc gggccagaca gctggaaaag gagcttctgc agtcacagaa gatggaggcc
     4261 gtcgggagac tcgccggagg catagcacat gatttcaaca atatcatggg cgggatcatg
     4321 gggcatgcgt cgctgctccg agtcgacgcg acggaaggaa ctgacctcta ccagggactc
     4381 gacacaatcg agcgtgccgc gagacgcgcg gccgatctca ctcgccggct cctgtccttc
     4441 tcccgcaaca gcaccccgac cgtcaccgac gtcgatgtcg ggggagtcat tgccgatacc
     4501 ctctcgatcg ccatgccggg cttcgacaag cgcacgaccc tggtacagaa cgtctcttct
     4561 gatctggcct acgttccggg agaccggacg cagctggaag aggtcttcct caacctgatc
     4621 atcaacgcgc gcgatgccat gggccaccac gacggtacac tcaccgtcga cgctgacaac
     4681 attgccctcg acgctccctt ctgcatcagc catggagttc ctgccctgcc cgacggcgac
     4741 tacgtacagg tcagggtatc ggacaccggc tgcggcgtct cgcccgaagc cagagaacac
     4801 atgttcgaac cgttcttcac gacacggccc gaagggacgg ggctgggact ctccatcgta
     4861 tggcgtgtcg tccacgacca tggagggact gtcgtcgtga cctcggtgcc ggaccgggga
     4921 acgacgttca cgctgtacct tcccgctgtc tccagagccg aggcccaggg aagggctcgg
     4981 cagacccctg tctcgggttc actgccccgt tccgtgcggg gtgaagccat ccttatcgtc
     5041 gacgatgagg atgttgttcg cagtgtagcc gtcaaggtcc tctccgggct cggatatcgc
     5101 gtcatcgata cggccgatcc gatgaacgcg ctcgatatct acgcggattc ctggcaatcc
     5161 atcgatctgg tcctgatcga catgatgatg ccccacatga acggcaagga cctgttcgag
     5221 aagcttcgac agatcaaccc tgcggtcgca gcaatcctca tgtccggcta cgatgccact
     5281 gatacgcctt ttgccagcat ggggttcgtg gctttcgttc ccaaacccta cacgctccag
     5341 gaactggcca cccgcgtgcg tcagtctttg tccactccag ctgcccgggc atccagccca
     5401 tcggaggagc cgaccgcctg agaaccgcgc tcagcagatg agtcagccac ggcaaatgtc
     5461 cgcagtcccc tgaacgagcg gggggtgaca aggacaagca tcgctccgag gaacactata
     5521 ccgatgagtc cactgaccag gaaccccatg ctggcaccca gagcatccga gatcttgccc
     5581 gcgtagagcg ctccgatggg ggtcgaaccc acgaagacga agttgtaggc agccatgaca
     5641 cgaccgcgca tattgtcggg cgactgcatc tggagtgcag tgttgcacat cgtcgacaat
     5701 gccgccatgc cgaacccgca gaagacgagc aggagggcag tcaggtagaa gtttcgctgg
     5761 atacccacaa ggatcatcgc gaccgcgagg atgaacccgg aaccgtagac gtaggcggga
     5821 gttggttcca ggcgtgctcc tccgagggac atgagcactc cggcagccag agctcctgct
     5881 cccatcgccg acatcagtcc gccatagccg tctgactgca gccccagtgt cagctttgcg
     5941 aagcttggga tgaggatatt gaagttcagc aggaacaggc tgatggtgcc ggcaatgatc
     6001 aggagactca tgacgacctg atttctggca atgtaccggc cgccctcgcg gacttcctga
     6061 acgtagttgc ccttgctgct ctttgtcggc ttacggcctg ctctcatgat gagcaggctg
     6121 atgatgacgg caatgaaact gaccgagttc agggcgaatg tccagggaat gcccacggct
     6181 gcgatcagga ctccaccaat gccgggaccg atgacgcggg caatgttgaa catcgtactg
     6241 ttgagtgcga cagcattgac cacagcagac ctgtcattca cctgttcgcc tacaaatgcc
     6301 tgtctggtcg gccagtcgac agcactgatg aggccggtga cggcggcaat aaccagaacg
     6361 tgccaatact gcgcatgacc gccaaacgct acggcggcca gagcgccggc ggccaccgca
     6421 tacatgattt gtgtgaacaa caggatcgag cgcttcgggt accgatcgat gataggcccg
     6481 atgaataccg acaggagcag agacggaaga aactggatgc tggtcagcac ccccaacttg
     6541 agcggcgagt tcgtcagctt gagaacgagc caggac
//
LOCUS       DATMZD010000238         2572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_76255,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000238 DATMZD010000000
VERSION     DATMZD010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76255"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..465)
                     /locus_tag="VN478_04960"
     CDS             complement(70..465)
                     /locus_tag="VN478_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22055.1"
                     /translation="MSQNILFHIILMALGVVVLLAAGYIGKTDKGGKNLNTHKTFAAV
                     GIVLVLAEVVALILTKVLMPTLPHFYIAVAAVVFMLMAPIGGLLYLKAVPAKKAALRK
                     SHRFDAFVFFGLAGLAVVFGIISVLRMLR"
     gene            558..752
                     /locus_tag="VN478_04965"
     CDS             558..752
                     /locus_tag="VN478_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22056.1"
                     /translation="MGSRLRENDDKRQSRQVSAVLDAWVLYPVIPAHAGIQHCSDLGT
                     TTDAGLNRAGTPEKCSRTGV"
     gene            complement(772..>2572)
                     /locus_tag="VN478_04970"
     CDS             complement(772..>2572)
                     /locus_tag="VN478_04970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWQ22057.1"
                     /translation="TDEVNRLKLMVIAMTGVIGLMLTFFATLFMNRTVVYRIAALGKQ
                     VAQVGRTGDLSLRVHASGSDEISHLAEDVNTTLESLEQTQADKDRMARQLEQDQAQLR
                     DYFDRAHDLIFALDTHGRLTLVNHSACSVLGYAEEDLLGRSALDIVSPASRDFALTAS
                     GAIESGDTVATTTIDVLTRDGNRRTLDVSGQRLFSDGRLTGTFYIARDITDRLRMDHE
                     VLRNQALESLGTLAAGIAHDFNNVLTVVKGNLSLADLSADDPTELRHHVEVARIAAER
                     AQALASQLLTFSRGGAPATENVNVETTVREVARFALSGSNIALRIDVDETLPAVEADP
                     NQLFQVIQNIILNARDAMLSGGTLTIVIHGQTVSEEQPFGFAAPGECVVIEIMDTGPG
                     ITPEDMKRLFDPFFTTKPSGHGVGLATARSIARRHEGDLIVESVQGKGTTVRLCMPVA
                     QGPASQTESTETATTPGVTTGRVLVMDDEQTVADVTCAMARRLGYEAAAVADGRAALT
                     AYEQAMVDQHPFDLVIMDLTIPGGMGGREAVQRLLALHPEARVIVASGYSDDASIADY
                     TRCGFIASLPKPYTMQDLQRVLREASKPRPSEA"
BASE COUNT          474 a          776 c          827 g          495 t
ORIGIN      
        1 gcaacaagca tggtggtcgg ctggctcccg ctttcgcgcg aatgatggaa gacactcccg
       61 agtgtcaggc tacctcagca tcctgaggac gctgatgatc ccgaagacga ctgcgagacc
      121 ggcgagcccg aagaagacga aggcgtcaaa ccggtgcgac ttgcgcaggg cggccttctt
      181 cgcggggaca gccttcaggt agagcagtcc tccgatgggc gccatcagca tgaacacgac
      241 ggctgcgacg gcgatgtaga agtgcggcag agtgggcatg aggacctttg tcaggatgag
      301 tgccacaacc tctgccagga cgagcacgat gccgacagcg gcgaaagtct tgtgtgtgtt
      361 caggttcttg ccacccttgt ctgtcttgcc gatgtaccct gcggcaagca ggacgacgac
      421 gcccaacgcc atgagaatga tgtggaacag aatgttctga gacattcgaa cctcccgaat
      481 cgtgacgaac tgcctcagta tggcgcgtgc acagacgttt acaaggacat gcggctgcta
      541 ctcgtgagaa gaagagtatg ggttcccgcc ttcgcgagaa cgacgacaag agacagagcc
      601 gccaggtcag cgccgtcctg gacgcatggg tcctctaccc tgtcattccc gcgcatgcgg
      661 gaatccagca ttgcagcgac ctgggaacga cgacggatgc agggctcaac cgagcgggga
      721 cgccggagaa gtgctcaaga acgggcgtgt gaggacaggg gttgatgcct gtcaggcctc
      781 cgatggcctg ggcttcgacg cttcgcgcag gacgcgctgc aggtcctgca tcgtgtaggg
      841 cttggggagt gacgcgatga aaccgcaccg cgtgtagtcg gcaatcgagg catcgtcgga
      901 gtagccgctg gctacaatga cgcgggcctc gggatggagc gcaagcagcc tctggactgc
      961 ttcgcgaccc cccatgccac ccgggatggt caggtccatg atgacgaggt cgaaggggtg
     1021 ctggtcgacc atcgcctgtt cataggccgt cagcgcagct ctcccatccg ccacggcggc
     1081 cgcctcgtag cccagacgcc gggccatcgc gcatgtcacg tcggcaacag tctgttcgtc
     1141 atccatgacc aggacgcggc ccgtggtgac gcctggtgtc gtcgcagtct ccgtcgattc
     1201 ggtctgactg gcaggcccct gggcgactgg catgcacagc cggaccgtcg tccccttgcc
     1261 ttgtaccgac tcgacgatca ggtctccctc atgccgcctg gcaatggatc gtgccgtggc
     1321 caggcctacc ccgtgcccgg agggcttggt tgtgaagaag ggatcgaaga ggcgtttcat
     1381 gtcttctggc gtgatacctg gaccggtgtc catgatctcg atgacgacac actctcctgg
     1441 ggcggcaaat ccaaaaggct gctcctccga cacggtctga ccgtgaatga cgatggtcag
     1501 tgttcctccc gacagcatcg cgtcgcgcgc attcaggatg atattctgga tgacctggaa
     1561 caactggttg gggtcagcct ctacggcggg cagggtttcg tcgacgtcga tcctcagtgc
     1621 tatgttggag ccactcaggg cgaacctcgc cacttcacgg accgtagtct cgacgttgac
     1681 gttctccgtt gctggagcac cgccgcggga gaaggtcagc agttggctgg cgagagcctg
     1741 agcgcgctct gctgctatgc gggcgacttc cacgtggtgc cgcagctctg tgggatcatc
     1801 tgcagacagg tctgccagtg agaggttccc cttgaccact gtcagcacgt tgttgaagtc
     1861 gtgggcaatg ccggcggcaa gcgtgccgag cgactccagg gcctggttgc gcagcacctc
     1921 atggtccatg cgcagccgat cagtgatatc ccgggcgatg tagaacgtcc ccgtcagccg
     1981 gccgtcgctg aagaggcgct gcccactgac atcgagagtc cgacggttcc cgtctctcgt
     2041 gaggacgtcg atggtcgtcg ttgcgaccgt atctccgctc tcgatcgcgc cgctcgccgt
     2101 gagcgcgaag tcgcggctgg ccggactgac gatgtcgagg gcgctgcggc cgaggaggtc
     2161 ttcctccgca tagccaagca ccgagcatgc cgaatggttg accagggtca acctgccatg
     2221 agtgtccagc gcgaagatga ggtcgtgtgc acggtcgaag tagtcgcgca gttgcgcctg
     2281 gtcctgttcc agctgcctcg ccatccggtc cttgtccgcc tgcgtctgct caagcgattc
     2341 cagagtcgtg ttgacgtcct cggccagatg cgatatctcg tctgagcccg aggcgtgtac
     2401 gcgaagcgag aggtcgccgg ttcttccgac ctgtgccacc tgcttcccca gtgcagcgat
     2461 ccggtagacg acggtgcggt tcatgaagag cgttgcgaag aacgtcagca tgagcccgat
     2521 cacacctgtc atggctatca ccatgagctt cagccggttg acctcgtcgg tc
//
LOCUS       DATMZD010000239         2748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_116846,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000239 DATMZD010000000
VERSION     DATMZD010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116846"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(210..1271)
                     /locus_tag="VN478_04975"
     CDS             complement(210..1271)
                     /locus_tag="VN478_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22058.1"
                     /translation="MNAGRQTVEWLYKNQLQIDEQWSVKTERGFTWWPYQHAQTITST
                     DEVEGPDGTPGAFVGVRTQVLRDLDLTDHLAISINELAREGANTMSGLMYDEKTRGLD
                     LCSLVPVHDDTREWMQQLISIAAVLQIGEAQALSSTLMKHYRVGGVEAASAHPTTGKR
                     PTADEMAEIARLLIIPMGAVPCAWTAEEFRETVDRHMQVPPALLATSGGDGFTVEFPF
                     GDVSSLCQAECDTHVALGNGLRVTQSFPVSEPSDAQGARFALLMNAAELANKPSGYGF
                     GSYSYREGLICFSTFFPNFIHKPGLLRNLYFAAARRARALSVMLTGKDWTVDSFDFRH
                     SSAGGALFGPPPAVQPEKT"
     gene            complement(1312..1728)
                     /locus_tag="VN478_04980"
     CDS             complement(1312..1728)
                     /locus_tag="VN478_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22059.1"
                     /translation="MSSRQVEWLRENVEEALRTLELSSRAVQAATDCERAAREALCRA
                     RVRLDEQRALDLREQKQAKVKLSVNPSTAKNQGGLPTKPKKRRTSISSLQSRALWKPN
                     PIETDRGGRHHLVKDEPPRAPLARLSHDDARDLWNN"
     gene            complement(1718..>2748)
                     /locus_tag="VN478_04985"
     CDS             complement(1718..>2748)
                     /locus_tag="VN478_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22060.1"
                     /translation="LLRDTNVLKRRDIEYLSTSGVSARSFAFTPGHLGDTATTKEAAM
                     ETREQDQLRVDTGETLRNPLLAFIGWHYYAPPAPGQAPSVAWQADIEGHALWLPRLAK
                     RLHMDELSLRSALYDVASLSTPRRADLRDSTICHVCPLAGELGTAIRRQYDEYCAAIV
                     WARLLTNRHILVVDGVACYDAYALAAALNWFTRTGWKYRLMDARLATTRWNRRVYTTE
                     EAISCWLAGKPCPDAPNLAAAVCAAGSARKLTVATVSKLSGTFGRTWVTFHGERYIWR
                     KEATRLYHVSARALRRARREGKLQACIVYAEVLIPFESLRQFVKWWYAHGCTWRGAKK
                     AKVANDEQ"
BASE COUNT          488 a          871 c          821 g          568 t
ORIGIN      
        1 gggccaaggg atcgtgtggg tcgtccatca ccagttcctc gaactgccgc tgtgcgctct
       61 cgaggtcccc tgcctgcact gcggcaagag cttgtcccag tctcaactgc ttgtcaacgc
      121 acttttcgct ttctctgctc atgatgtcag tataggccca caaggccgat agccaacacg
      181 acgtgcgcgg gctgcccttg tcacccgcgt cacgtcttct ccggctgaac ggctggaggc
      241 ggaccaaaga gtgccccgcc ggctgagctg tgcctgaagt cgaatgaatc gaccgtccag
      301 tccttgcccg tgagcatcac agacaacgct cttgcccgcc gggccgcagc gaagtagaga
      361 ttgcgaagga gtcctggttt gtggatgaag ttggggaaga acgtcgagaa gcagatgagt
      421 ccctctcggt aggagtacga gccgaacccg tagcccgagg gcttgttggc aagttctgca
      481 gcattcatga ggagcgcgaa tctggctcct tgtgcgtcac ttggttctga cacggggaag
      541 gattgagtca cgcggagtcc attcccgaga gcgacatgtg tgtcgcactc cgcctggcac
      601 agagaagaca catcaccgaa cgggaactcc accgtgaaac cgtcgccccc agatgtggcc
      661 aacagggcag gcggtacttg catgtgacga tccaccgttt ccctgaactc ctcggctgtc
      721 catgcgcacg ggaccgctcc catcgggatg atcagcaacc gggcgatctc ggccatctcg
      781 tccgcagtcg gcctcttgcc agtcgtggga tgtgcgctgg ccgcttcgac acctccgacg
      841 cggtagtgct tcatcagtgt cgaggacagt gcctgtgcct ctcctatctg gaggacggca
      901 gcgatgctga tgagttgctg catccactcg cgcgtgtcat cgtgcacggg gaccagagaa
      961 catagatcaa gaccccgcgt cttctcgtcg tacatcagtc cagacatcgt gttggcacct
     1021 tctctggcca gctcattgat gctgattgcc agatgatcgg tcaggtcgag atcgcgcagg
     1081 acctgcgtcc gcactccgac gaacgccccc ggcgtcccgt ctggaccctc cacctcatcc
     1141 gtggacgtga tcgtttgagc atgctggtac ggccaccagg tgaacccacg ctccgtcttc
     1201 actgaccact gctcgtctat ctgtaactgg ttcttgtaga gccactcgac ggtctgtcta
     1261 cctgcgttca taggatcctc cttctccgtg gcatccactc ctgtgagaag cctagttgtt
     1321 ccagaggtcg cgcgcgtcat cgtgcgacaa tcgagccaga ggagctctcg gcggctcatc
     1381 cttgaccaga tggtgcctcc ctccacgatc agtctcgatg ggattcggct tccacagcgc
     1441 acgactctgt agtgaagaga tagatgtcct ccgtttcttc ggcttggtgg gcagtccgcc
     1501 ttgattcttg gcagtgctag ggttcacaga aagctttacc ttggcttgct tctgctccct
     1561 taggtcgagc gctcgctgtt cgtccagtct gacacgcgcg cgacatagtg cctcgcgagc
     1621 cgccctctcg cagtccgttg cggcctgaac ggctctggag gacagctcca gcgtccggag
     1681 tgcctcttcg acgttctcgc gaagccattc gacctgccta ctgctcatcg ttcgccacct
     1741 tggccttctt ggcccctctc cacgtgcaac cgtgagcata ccaccacttc acgaactgcc
     1801 ggagagactc gaacggaatg agaacctcgg catacacgat acacgcctgg agcttcccct
     1861 cccggcgtgc acggcgcaaa gcccgggccg agacatgata cagcctcgtg gcctccttgc
     1921 gccagatgta gcgctcgccg tggaaggtga cccacgtccg gccgaacgtg ccggagagtt
     1981 tggacacggt ggccaccgtc agcttgcggg cacttcctgc cgcgcacacg gcagcggcaa
     2041 ggttcggcgc atcggggcac ggctttcccg cgagccagca gctgattgcc tcctcggtcg
     2101 tgtacacacg tcggttccag cgtgtcgtgg ccagccgggc atccatgagc cgatacttcc
     2161 agccagtgcg ggtgaaccag ttcagcgccg ctgcgagggc ataggcgtca tagcatgcca
     2221 cgccgtccac aacaaggatg tggcggttgg tcagcaggcg tgcccagacg atggcggcgc
     2281 agtattcatc gtattggcga cgtatcgcgg taccaagttc gccggcgagc gggcagacgt
     2341 gacagatggt actatcgcgc aagtcggcgc gacgtggtgt cgacagtgat gctacgtcat
     2401 agagagccga gcgcaggctg agctcgtcca tgtggagccg cttggccaag cgcggaagcc
     2461 agagcgcgtg cccctcgatg tcggcctgcc aggctacgct gggcgcctgc cccggagcag
     2521 ggggcgcgta gtagtgccag ccgatgaatg ccagcagggg gttccgcagg gtctctcctg
     2581 tgtcgacgcg cagttggtcc tgttctctcg tttccatggc tgcctccttc gtggtcgccg
     2641 tgtcgccgag atgcccaggg gtgaacgcaa acgacctcgc gctcacgccc gaggtcgaca
     2701 aatactcgat gtcccggcgc tttagcacat ttgtatcgcg gagcaagt
//
LOCUS       DATMZD010000240         2801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_66654,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000240 DATMZD010000000
VERSION     DATMZD010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66654"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..1074)
                     /locus_tag="VN478_04990"
     CDS             complement(10..1074)
                     /locus_tag="VN478_04990"
                     /inference="COORDINATES: protein motif:HMM:NF019142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HWQ22061.1"
                     /translation="MDTTRLVRVFHRRKALLFAVTGVLLGGLFLWTAGVHAYVVDDAG
                     NKTRIVAFHSMSVSTVLTKANIRSLRPEDVVTPVVDTVIPNGEIAIVRAKPVTLTIDH
                     TSETIYSTVTTVGELLADNGVKLKAEDKVSLDLADPVPYNDDIVVKRTTYAYTSKTET
                     VAYKRVYEKNPFMEVGRSYISVMGQNGILKKTIKVTKVSQQAVGSTVVKTQTIKPVVH
                     EVYDVGTARLDLKPVRTYTMSATAYSNRRSTVLDGEDHLYGYSGLWLSYGTCAVDKRI
                     IPMGTVLYVEGYGYAVAADTGGAIRGNRIDLFFNSYNDACRWGLRSVKVYVLPVTRTE
                     LYRKLGVSQNAVVKTLYTGE"
     gene            complement(1187..1915)
                     /locus_tag="VN478_04995"
     CDS             complement(1187..1915)
                     /locus_tag="VN478_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006009200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HWQ22062.1"
                     /translation="MEYQGTVVRPEDGIRAFFNRVYGWMAGGLALTAAIAWLVGTSTA
                     MQQVIFGNPIVLILLIVAEFGLVIGLSRAINRMSPQTAGLLFLAYAAVNGLTLSSIFM
                     VYASGDITMAFVASAGLFLAMSLIGSATHMDLSRLGNIALVGLFAIIGMSIINIFTKS
                     SGLEFLISIVGIVVFLGLTAWDTQKLRRMALNLDPAGDNPITMAGGNPQAEKAAVIGA
                     LSLYLDLVNLFLFVLRFFSGRRRS"
     gene            2251..>2801
                     /locus_tag="VN478_05000"
     CDS             2251..>2801
                     /locus_tag="VN478_05000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HWQ22063.1"
                     /translation="MDEQSDKHSAGQHDDFLTCAVPSDAERLAAHQWQEFLESSPDPM
                     WIKDTQGRYVAVNKAYLRADPGQTQDVIGKTDFEVQSRERAEVYVADDQIAIHDGVCE
                     HEFGSVDHEGNLKYYNTKKTALRDANGVLTGILGQARDVTHQRSLELALALENRRSRL
                     FQNILEAAVTATSVGAFLADVLT"
BASE COUNT          598 a          850 c          800 g          553 t
ORIGIN      
        1 gacgtacgcc tattcgcccg tgtacagcgt cttgacgact gcattctggc tgactccgag
       61 cttgcgatag agctcggtcc gggtgacagg caggacgtag actttcacgc tgcgaaggcc
      121 ccatctgcaa gcatcgttgt aggagttgaa gaacaggtcg atcctgttgc cccggatcgc
      181 gccgcccgta tcggcagcca ccgcataccc gtatccctcg acgtacagca ctgtgcccat
      241 ggggatgata cgcttgtcga ctgcgcaggt gccgtagctc agccacagcc ctgaatagcc
      301 gtagaggtga tcctcgccgt caagaaccgt ggagcgcctg ttcgaatagg cggtagcaga
      361 catcgtgtag gtgcggacag gcttgaggtc cagccgggct gtcccgacgt catacacctc
      421 atgaacgacc ggcttgatgg tctgtgtctt cacgacggtt gaaccgacgg cctgctggga
      481 gaccttggtc accttgatcg tcttcttgag gatgccgttc tggcccatga cgctgatgta
      541 gctgcgcccg acttccatga acggattctt ctcatacacg cgcttgtagg ccaccgtctc
      601 ggtcttgctg gtatacgcgt aggtcgtgcg cttgacgacg atgtcgtcgt tgtagggcac
      661 gggatcggcc aggtccagac tgactttgtc ctccgccttg agcttgacgc cgttgtccgc
      721 caggagctct ccgaccgtcg ttacggtcga gtagatcgtt tcgctggtgt gatcgatggt
      781 gagtgtgacc ggcttggcgc gcacgatagc gatttcccca ttggggatga ccgtgtcgac
      841 aaccggtgtt accacatcct ccgggcgcag ggaacgtatg ttcgccttcg tcaggacggt
      901 actcaccgac atgctgtgga acgcgacaat ccgcgtcttg ttgcctgcat cgtctacaac
      961 atacgcgtga acgcctgcag tccagaggaa cagcccacca aggaggactc ccgtcacagc
     1021 aaagagcaga gcctttctcc tgtggaacac cctcacgagc cgtgttgtgt ccatagtgcc
     1081 tagattatac caccgcccga ctgcctgcaa aggttccatt gaacaacaaa cggccagact
     1141 gccccgtatg agcggtccgg ccgcattcac aacagatgcg cctgggtcag ctgcgccttc
     1201 gcccgctgaa gaacctgagg acaaacagaa acaggttgac caggtccaga tacaggctca
     1261 aagcaccgat gacggctgcc ttctctgcct gcgggttccc cccggccatc gtgatcgggt
     1321 tgtcgcccgc agggtccagg ttgagggcca tccggcgcag cttctgcgtg tcccaggcgg
     1381 tgaggccaag aaacacgaca atgccgacaa tgctgatgag gaactccagg cctgaactct
     1441 tcgtgaagat attgatgatg gacatgccga tgatggcgaa caacccgacc agggcgatgt
     1501 tccccagcct ggacaagtcc atgtgtgtgg ccgacccgat cagcgacatg gccagaaaca
     1561 agcctgcgga agctacaaat gccatggtga tgtcgccgct cgcatacacc atgaagatgg
     1621 aagacaacgt cagtccgttc acggcggcgt acgccaggaa caggagccct gcagtctgtg
     1681 gagacatcct gttgatggcg cgcgagaggc cgatgaccag accaaactcc gcaacaatga
     1741 gaagaatcag cacaatcggg tttccaaaga tcacctgctg catcgccgtg ctagtgccga
     1801 caagccaggc aatagccgca gtgagcgcga gcccaccggc catccagccg tagacgcggt
     1861 tgaagaatgc ccggatacca tcctccggac gtacgacagt cccctgatat tccatggaca
     1921 acatctcctt tcctgcgcat ctggttccac cagacggtcc cagatgcgga gcaatcatag
     1981 gcggcgactt tgccttgtca agcgccgttc gacacagtga caatgctccg tcttccacct
     2041 atagatacgc tactcccggg cgtttcgact tcgcggattg tgaagtcctg gaacgtcttc
     2101 caggggaaca agaaggcccg gcgaacgacc agaaccatca ggttccctgc acattgtccc
     2161 ggtgtattct ctggttgatt ttccaaagtg gccatcgtat acttgactaa cagcatgcca
     2221 ggcgacaggt ctgtgctgca ggaggccatc gtggacgagc aatcagacaa gcactcagcg
     2281 ggacagcacg atgacttctt gacatgtgca gtaccgtcgg acgccgaacg actcgccgcg
     2341 catcagtggc aggagttcct cgaatcctcg cccgacccca tgtggatcaa ggatacccag
     2401 ggccgctatg tggctgtcaa caaggcctac ctgcgcgccg acccgggtca gacacaggat
     2461 gtcatcggca agaccgattt cgaggtccaa tcacgcgaga gggccgaggt atacgtcgcc
     2521 gacgaccaga tcgctatcca cgacggcgtc tgcgagcatg agttcggctc ggtggaccac
     2581 gagggaaacc tcaagtacta caacaccaag aagaccgcgc tccgagatgc caatggtgtg
     2641 ctgaccggca tcctcggcca ggcccgggac gtcactcatc agcggagcct tgaacttgcc
     2701 ctcgctctgg agaaccgccg gtcccgtttg tttcagaaca tcctcgaggc ggcggttacg
     2761 gcaacgagtg tcggtgcgtt cctcgcggac gtcctcacca t
//
LOCUS       DATMZD010000241         3290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_17248,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000241 DATMZD010000000
VERSION     DATMZD010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17248"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="VN478_05005"
     CDS             complement(<1..371)
                     /locus_tag="VN478_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent exopeptidase M28"
                     /protein_id="HWQ22064.1"
                     /translation="MTDLKTAVLQHLHHLAGEIGPRPIGSASNKAAGAYIASVLTSSG
                     YTVEKQEYTCPDWALQGVSLEQGGSPVDVRANGYAPSCDITAPAVVAATPAELDGLDC
                     AGRICIIHGDLTRREELDPPYA"
     gene            complement(451..1257)
                     /locus_tag="VN478_05010"
     CDS             complement(451..1257)
                     /locus_tag="VN478_05010"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HWQ22065.1"
                     /translation="MGKGTPTTRRAARKAGRSHARHFTTSQVADIAGTHPNTVRLYEK
                     LGFLRRLPRSAGGYRLFSKGDVDQMCFARTALNGGWPGRDVRESAIALVRRAVSGDLC
                     TALDQARQHLGLVQAERKQAEDVAAFLARWAHGEAVPAGPGCPRRIGDVAREIGLSPD
                     SLRGWERDGLLRVDRDDHGYRLYGTADVSRLHVIRLLLRAGYSSMAVLRMFLRLDSGE
                     AVDVRNVLDTPRPDEDALTAADRWLTFLAEQEQRAAKLVQMLTGMASTRA"
     gene            1380..2000
                     /locus_tag="VN478_05015"
     CDS             1380..2000
                     /locus_tag="VN478_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22066.1"
                     /translation="MDQQPSSIPDSTTPRPTEVRLKLPAAQLQGRPAAFDPTQIAGAA
                     AYAILLYLGILYGLSLIGATAGMASGLQTTIALIGSLLLTGALFGVMAVLRDRLAHKT
                     AGATPEEVIRLDDALRWLVPGDLMTLYAGPMTILRLNLLDLLAAADSAQRMVAAAAYA
                     EWPAPLDHLAAATFTAYYGISNADAEPFTAQDAAVLGQYLAERDQA"
     gene            2117..2545
                     /locus_tag="VN478_05020"
     CDS             2117..2545
                     /locus_tag="VN478_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22067.1"
                     /translation="MELNKDRGPIYSNYDRDRSRDYSPVIDYAAPPRSSEEMEFMLAH
                     KIAPGVAEAGDMQSATTQRYQSGTAPLPAFLQALSDSMAQRTQGQAGNLQSPAQQKTS
                     WSYQGRTAHRNQSQSKAALIGVLIWVLFILASMLMSIFGR"
     gene            2571..3002
                     /locus_tag="VN478_05025"
     CDS             2571..3002
                     /locus_tag="VN478_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22068.1"
                     /translation="MLLSWLLSDTWLMTVPARVADILLMAITLWLASRWCRSRYGTLG
                     RALLVSSLNGILGTGLYALFVWVRDTGALPWRNPLWLVEMFLLAQSVLFVLLVLIAYR
                     RDRWRSLNVALVALVLTGLVETGLILFVSLFAPHTTSLPRP"
BASE COUNT          609 a         1083 c          994 g          604 t
ORIGIN      
        1 gcgtagggcg ggtcgagctc ctcgcgccgg gtcaggtcgc cgtggatgat acagatacga
       61 ccagcacaat cgagtccgtc gagttcagcc ggtgtcgcgg cgacgaccgc aggcgcagtg
      121 atatcgcagg acggcgcgta cccgttggcg cggacgtcga caggcgaccc gccctgttca
      181 agggaaactc cctggagagc ccagtcgggg cacgtgtatt cctgtttctc aaccgtgtat
      241 ccactggacg tgagtacact tgcgatgtat gcacctgcag ccttgtttga cgcagatccg
      301 atcggacgag gtccaatctc acctgcaagg tgatgcagat gctgaagcac agcagttttg
      361 aggtcagtca tccggtgttc ctcctcgagt acagaatgtg gcctgcgcag atctactacc
      421 attctacgac cctggagttc taacggaggg ttatgcccta gtcgatgcca tccctgtcag
      481 catctggacg agctttgccg cccgctgctc ctgttcggcc aggaaggtga gccatcggtc
      541 tgccgctgtc agcgcatcct catccggtcg cggcgtgtcc agcacgttcc gcacgtcgac
      601 cgcctcgccg ctgtccagtc tcaggaacat gcgcaggact gccatggagc tatacccggc
      661 acgcagaaga agacgaatga catggagacg agacacatcg gcagtcccgt agaggcggta
      721 gccatggtcg tcgcggtcca cgcgcagcag cccgtcgcgc tcccagccgc gcagggagtc
      781 cgggctgagc ccgatctcgc gagccacatc accgatgcgc cgcgggcaac ctggcccggc
      841 tgggactgct tcaccgtgtg cccatcgcgc aagaaaggcc gccacgtcct cggcctgttt
      901 cctctcggcc tgcaccaggc caaggtgctg ccgtgcctgg tccagcgcgg tacacaggtc
      961 acccgagacc gcccggcgga cgagagcgat tgccgactcg cgtacgtcgc gccccggcca
     1021 gcctccgttc agggcagttc gcgcgaagca catctggtcg acgtcgccct tcgagaacag
     1081 gcggtatccc ccagcactgc gaggcagtct ccgcaagaac cccaacttct catacaggcg
     1141 gaccgtgttc gggtgtgtcc cggcgatgtc ggcgacctga gacgtcgtaa agtgccgggc
     1201 atgtgaacga ccagccttcc gcgctgcccg cctcgttgtc ggcgttccct tgcccatggt
     1261 atacctccct gcactatatt gtaacacctg cgacactgag aaaagcagtg cgtcgcgtct
     1321 gttgaaccgg gacgactccg ccctaagatg agggcacgac acatcgatga ggtgagtgca
     1381 tggatcagca accatcatcc ataccagaca gcacgacacc gcggccgacc gaggtccgac
     1441 tgaagctgcc ggctgcacag ctgcagggca ggcctgcggc attcgacccg acccagattg
     1501 ccggcgccgc cgcctacgcc atccttctgt acctcggcat cctctatggg ctcagcttga
     1561 taggcgccac ggccggaatg gcctccggcc tgcagaccac gatagcgctt atcggaagcc
     1621 tgctgctgac gggcgcgctc ttcggcgtca tggccgtcct tcgtgaccga ctggcacaca
     1681 agacggcagg agcaactccc gaggaagtca tcaggcttga cgacgccctc cgctggctgg
     1741 taccaggcga cctcatgacg ctctacgcag gccccatgac catcctgcgt ctcaacctcc
     1801 tggacctgct cgcggctgca gacagcgcac agcgcatggt ggccgcagcc gcgtacgccg
     1861 aatggccggc gccgcttgat catctggcag cggctacatt caccgcgtac tacggcattt
     1921 cgaacgcaga cgccgaaccg ttcactgccc aggacgccgc ggtactcggg cagtacctcg
     1981 cagagcgcga ccaggcctga tcatgccggc ttcgaaggag acgcgattgc gtgactgcaa
     2041 atcgacaacg tgctagaata aggtactgga atggcagtga tgccggggaa cgtgtgcggc
     2101 aagggggatg acagcaatgg aactcaacaa ggaccgagga ccgatctact cgaactacga
     2161 ccgtgaccgt tcacgggatt acagtccggt catcgactat gccgcaccac cacggagcag
     2221 cgaggagatg gagttcatgc tggcgcacaa gatcgcgccg ggtgtggccg aggcgggcga
     2281 tatgcaatct gccaccaccc agaggtatca gtcgggcaca gcgccccttc ccgcctttct
     2341 acaggccctg tccgactcga tggcacagcg gacacagggt caggccggga atctccagag
     2401 ccctgcacag cagaagacgt catggtcata ccagggccgg actgcacaca gaaatcagtc
     2461 tcagagcaag gcagccctga tcggcgtcct catctgggtc ctgttcatac tcgcctccat
     2521 gctgatgagc atcttcggcc gctagtctcc agcaggtcct gggccttcct atgctactct
     2581 cctggctgct ttccgacacc tggctgatga ccgtgcccgc ccgcgtggcg gacatcctgc
     2641 tgatggcgat cacgctgtgg ctggcatcac ggtggtgtcg cagcagatac gggacgctcg
     2701 gcagggctct gctggtctcc tcgctcaatg gaatcctcgg gacagggctg tatgccctgt
     2761 tcgtctgggt gcgcgacacc ggcgcgctgc cctggcgcaa tccgctgtgg ctggtagaga
     2821 tgttcctcct tgcccagagt gtgctgtttg tcctgctcgt cctcatcgcg taccggcggg
     2881 accggtggcg ttcgctgaac gtggcactgg tcgcactcgt cttgacgggt ctcgtcgaga
     2941 caggtctgat cttgttcgtg tcgctcttcg caccccatac cacaagcctg cctcgcccat
     3001 agagtgccga cccggcgcgc ttgactcgcg cgcccggagg tgctgtccaa catcctgaag
     3061 ccgcccgtct tgcaccgagc gacggcagca ctatgatgtg cgaggcgtct ggagggtgag
     3121 tctcgaccct ctccgtccca aacgaaacca taccacgtcc cagggggaca agtacgtgaa
     3181 accatactcc ggtcggatcc tgtgggtcga cctgacgaca ggcgcttgta ccgacgagga
     3241 cgttccggag ccggtgtacc gcgcttacct cggcgggctc ggacttgctg
//
LOCUS       DATMZD010000242         2755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_26931,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000242 DATMZD010000000
VERSION     DATMZD010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26931"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..972
                     /gene="cdaA"
                     /locus_tag="VN478_05030"
     CDS             58..972
                     /gene="cdaA"
                     /locus_tag="VN478_05030"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HWQ22069.1"
                     /translation="MTFLQKFLADLFVNLTPGRGLSIVIDVALTSLLFYAILRLISNT
                     RALQLAQGLLIYYLLVYGVEWLSLKTGLLALNSVFSWLRGFSTSFLPLLLVMVFQPEL
                     RRMLGRLGSSKLVSSDTRTQFMDVHDWEESVDEIVKSVKYLSMNRIGALICMQRQTGL
                     EDYVETGVRLDALVRAETLSTLFYPNSALHDGGVIIASNRVVAARCLFPLTASADLER
                     GLGTRHRAAIGITEETDSIVVVVSEQTGVISLAVRGKLTRYLSPLELRGMLLVMTKPI
                     VSEHRFSLSGLFHKRKSDGQQGGGDGQA"
     gene            959..2182
                     /locus_tag="VN478_05035"
     CDS             959..2182
                     /locus_tag="VN478_05035"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HWQ22070.1"
                     /translation="MAKRNIGAMLFNSKIISVLLAVLLWVYVVGVRGPDTTKSVETQL
                     IAVNVPQGYVLSGSLPTVTVTLRGPMNTLWNVTSGYVTPTVDLRGRVEGSFIASVQAQ
                     VAGLAGVTVEAVTPKEVNVQLERLESLTIPVHAEVSGALPLGLVLGTIHVEPDTIEVS
                     GPGSLVSQVKEAALVVALDKLGTTAGGNLSVAGDVVAYDASGNVVDGVLLSPRSAVAI
                     LPILDAATLKTVPVVPSITGYPMSGYAVATASCTPAIVMVTGTAAVLGQVQTLSTTAL
                     DISGDSGVLTKQVDLVLPPGVSLVTGSNAVSCRVVIEQVVVLAIPDVSIEVRGAGSEW
                     KVSLGTTSASVLISGTNSAIMALRSSQIKVYIDVSRPALSDGSYSVSVDGLMQGVVSA
                     MVTPTSIVADVQKGP"
     gene            2333..>2755
                     /locus_tag="VN478_05040"
     CDS             2333..>2755
                     /locus_tag="VN478_05040"
                     /inference="COORDINATES: protein motif:HMM:NF013660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HWQ22071.1"
                     /translation="MAKRILGADKAGFLGTLDVPAMGVLPIALGSATKLISFLPPSRK
                     EYIGELVLGVSTVTDDMDGEVTERRGTAGIGRESVTKAIETVAVWTEQVPPHVSAKQQ
                     DGVRGYKAMRGEGRLIDFAAVPVAVQRLAVLQERQDMDL"
BASE COUNT          519 a          761 c          866 g          609 t
ORIGIN      
        1 acggatcacg gtttggacct gaatccaagc gcatgatcgt tattgctgag aggtgacatg
       61 actttcctgc agaagttcct cgcggacctg tttgtgaatc tcacgccggg acgtggcttg
      121 tccatcgtca ttgacgttgc actgacctcg ctcctcttct atgccattct gaggctcatc
      181 tcgaacacga gggctctgca gctggcgcag ggtcttctga tctactatct gctcgtctac
      241 ggggtagagt ggctcagtct caagaccggc ttactggcgc tcaacagtgt gttcagctgg
      301 ttgcgtggct tctcgacgtc atttcttccc ctattgctcg tcatggtctt ccagcccgaa
      361 ctgcgcagaa tgctcggtcg actggggagc agcaagcttg tgtcctccga cacacgcacg
      421 cagttcatgg acgttcatga ctgggaggag tctgtcgacg agattgtgaa gagtgtgaag
      481 tacctgtcga tgaatcggat cggagctctc atctgtatgc agaggcagac gggtcttgag
      541 gactatgtag agacgggagt ccgccttgat gcgctcgtgc gggcggaaac cctgtcgacg
      601 ctcttctacc ccaacagcgc tctccatgat gggggcgtca ttatcgcgag caatcgagtc
      661 gttgctgcaa ggtgtctgtt tcccctgacg gcgagcgcag acctggaacg tggcctggga
      721 accaggcacc gcgctgctat cggcattact gaggagaccg actccatcgt tgtcgtagta
      781 tccgaacaga cgggggtcat ctctctggca gtgcgaggca agctgacccg ctacctgagt
      841 ccacttgagc tgagaggcat gctgctggtc atgacaaagc ccatagtcag tgaacacagg
      901 ttcagcctta gcggtctctt tcacaaacga aagtcggacg gacagcaggg aggcggagat
      961 ggccaagcgt aacatcggtg ccatgttgtt caactcaaag atcatctcgg tacttctggc
     1021 cgttcttctg tgggtctacg ttgtgggtgt ccggggccct gacacgacca agtccgtcga
     1081 gacgcagttg atcgccgtga atgtacccca gggatacgtt ctgtcaggga gtcttcctac
     1141 agtcacggtg acgctgcgcg gccccatgaa cacgttgtgg aacgtcacga gcggctacgt
     1201 gactcctact gtcgatctgc gagggcgagt cgagggttcg ttcatcgcat cggtccaggc
     1261 ccaggtcgcc gggcttgccg gggtgacggt ggaggcggtg accccgaaag aggtgaacgt
     1321 tcagctcgag cgactggaga gcctcaccat tccagtgcac gcagaggtga gcggcgcctt
     1381 gccgttgggc ctcgtcctcg ggaccataca cgtcgaacca gacacaatcg aggtgtcggg
     1441 tccgggctcg ctcgtgagtc aagtgaaaga ggcagctctg gtcgtggcgc ttgacaagct
     1501 tggaacgact gcaggcggca acctcagcgt cgcaggggac gtcgtcgcct atgatgccag
     1561 cggaaatgtc gtagatggag tgctgctgtc tccaaggtcc gccgttgcca ttctgccgat
     1621 tcttgatgca gcgaccctga agacggtgcc ggtcgtgccg agcatcactg gctaccccat
     1681 gagtggatac gccgtggcga ccgcctcgtg tactcctgca atcgtcatgg ttactggaac
     1741 cgcagccgtg ctgggtcagg ttcagaccct ttcgacgact gcactcgata tctcgggaga
     1801 ctcgggagtc ctcaccaagc aggttgacct ggtccttcca cctggagtct cacttgtgac
     1861 cggcagcaat gccgtgtcgt gccgagtcgt cattgagcag gttgtcgtcc ttgctatccc
     1921 cgacgtatcg attgaggttc gtggagctgg atcggaatgg aaggtttcgc ttggtacgac
     1981 atccgcgtcc gtcctcatca gtgggacgaa ctccgctatc atggctctga ggtcttccca
     2041 gatcaaggtg tacatcgacg tcagccggcc ggcgctcagc gacggcagct actcagtttc
     2101 ggtggacgga ctcatgcagg gagtcgtttc cgcaatggtc acgccgacct ccatcgtggc
     2161 ggatgtgcag aagggaccct agcagacgaa tgctggtcct gatggcgctg gccttgagca
     2221 ggacatgccc gggacgcctt gtatcggcgt cggacgattg agtatggtgc atcccgaggg
     2281 cttcttcgcg ttgttcaagc ccagcggcct tagcagcgct ggttcactta gcatggctaa
     2341 gcgaatcctg ggggctgaca aggcgggttt cctgggtacg cttgacgttc cggcgatggg
     2401 agtgctcccg atcgcccttg gctctgcgac caagcttatc agcttccttc cgccgagccg
     2461 gaaggagtac attggtgaac tcgtcttggg ggttagcacc gtcacggatg acatggacgg
     2521 ggaagtgact gaacgcaggg ggaccgcggg catcgggcgt gagtcagtga cgaaagcgat
     2581 tgagacagtg gcggtatgga ccgagcaagt ccctcctcac gtctcagcca agcagcagga
     2641 tggggttcgg ggatacaagg cgatgcgcgg tgagggccgc cttatcgatt ttgccgcggt
     2701 cccggtagcc gtacagcggc tcgctgtgct tcaagaacgg caggacatgg atctt
//
LOCUS       DATMZD010000243         3185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_117159,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000243 DATMZD010000000
VERSION     DATMZD010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117159"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="VN478_05045"
     CDS             <1..484
                     /locus_tag="VN478_05045"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HWQ22072.1"
                     /translation="LTGLYNRRYVDTILDQQYGLFKRHFQRFGLVMIDVDKFKGINDR
                     FGHLAGDEALKFVASVLQRTMRSMDFLARFGGDEFVIICPLVELEGIEKLSQRIVELV
                     HHSVLSSPTDPKDVIQLSVSVGGSMVDNKDTSASEVIARADEALYRVKRDGGDWYALG
                     "
     gene            complement(571..1785)
                     /locus_tag="VN478_05050"
     CDS             complement(571..1785)
                     /locus_tag="VN478_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017755891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HWQ22073.1"
                     /translation="MSSQSGEQKMQWAIRHMPILSSVAERFEKEQPFAGLNVVIALHL
                     EAKTANLACVMLRGGAKVAITASNPITTQDDVAQALAERGVTVFAKRGETPAEYKEYF
                     GNVLATRPNLLIDDGGDLVNAVHFERPELLPAIVGACEETTTGVIRDRALARAGKLSF
                     PVIGVNEGLCKHLFDNRFGTGQSAWDGILRTTNMNIAGKVVVVAGYGWVGKGVAMRAR
                     GLGAQVVVTEVDPVKAVEAHMEGYQVMPMLDAARIGEIFVTCTGDTKVITKEHFAVMK
                     DGPILCNAGHFDVEVDVQHLGNKDPEHHEVRHNIEEYVCDGKRFYVLGEGRLVNLACA
                     DGHPIEIMDLTFALQALSAEYLVRHQQSLPNELISVPGTIDDMASRAFLASRGLSIDT
                     LTEEQAQYLGSY"
     gene            complement(1859..2806)
                     /locus_tag="VN478_05055"
     CDS             complement(1859..2806)
                     /locus_tag="VN478_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWQ22074.1"
                     /translation="MNITQINLTIAQMLDYTTPILFAALCGVLSENAGVVNIGLDGIM
                     RFGAFFGLLGAFFSHSPWIGLLFGIAIGCLVGALHAFITIRWNGDQTVAGTAINVIAV
                     GLMTYLLEFVFSTTGYSPQLQNYFGAQAYRVHLPFLAKIPLLGAFSNLSFMIWLALIC
                     TILLHVLLYHTVLGLRIRTCGENPKAATTLGINVFKVRWFAVIAGAALAGIGGAALSI
                     SSFSGFSDSMPNGVGFIGMAAMIFGKWTPFGALGAALLFGTGQIVQSIIAVTGIAPKL
                     APGLTLILPYVLTLAVLAGFVGKSIAPAADGLPYYKEQR"
     gene            complement(2803..>3185)
                     /locus_tag="VN478_05060"
     CDS             complement(2803..>3185)
                     /locus_tag="VN478_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWQ22075.1"
                     /translation="VNRRIMQLMFFSGALAALGGCFFILGMQYQFPNTATMGYGWTGI
                     GVALIGQSEPLGVVLAGLFIGALRTGATSMQVANIPKTFSNIIEGMAVGFIALQVAVR
                     YYLVKILQHRRSRRNPSADREAAA"
BASE COUNT          642 a          899 c         1021 g          623 t
ORIGIN      
        1 gttgacgggt ctctacaacc gacgctacgt cgacacgatc ctggaccagc aatacgggct
       61 gttcaagcgg catttccagc ggtttgggct tgtcatgatc gacgtcgaca agttcaaggg
      121 tatcaatgac cggttcgggc acctggcggg agacgaagca ctgaagttcg tcgcctccgt
      181 cctgcagcga acgatgcgct ccatggactt cctggcccgc ttcggcggtg acgagttcgt
      241 catcatatgt ccgctcgtcg agctcgaggg gatcgagaag ttgagccagc gcatcgtgga
      301 gctcgtccat cattcggtcc tttcgtcgcc gacggacccg aaggatgtca tccagctctc
      361 ggtgtctgtc ggagggtcga tggtagacaa caaggacacg tcggcctctg aagtcatcgc
      421 gcgggcggat gaggcgctat atcgggtcaa gcgggacggc ggagactggt acgccctggg
      481 ctgagccaat ttctgagcac ttcgtgacca ggggccggac acggagtccg gccccctcgt
      541 ttgcatacag tctatgctgg tctctggcgt ctagtaggag ccaagatact gtgcctgctc
      601 ttcggtcagc gtatcgatgg ataggccgcg agaggcaaga aaggctctcg acgccatgtc
      661 gtcgatcgtc ccgggtacgc tgatgagttc gttgggcagc gactgctggt gcctgaccag
      721 gtactctgcc gacagtgcct ggagcgcgaa cgtcaggtcc atgatctcga taggatgtcc
      781 gtcggcgcat gccagattga caaggcgtcc ctcgcccagg acgtagaagc gtttgccgtc
      841 gcacacatac tcctcgatgt tgtgccgcac ctcatggtgc tcggggtcct tgttccccag
      901 gtgctgaacg tcgacttcga cgtcgaagtg tccggcgttg cagaggatag gcccgtcctt
      961 catgacggca aaatgctcct tcgtgatgac ctttgtgtca cccgtgcacg tgacgaagat
     1021 ctcgccgatc ctggccgcat cgagcatggg catgacctga tacccttcca tgtgggcttc
     1081 gacggctttg acggggtcca cttcggtgac gaccacctgg gcgccgaggc ctctggcacg
     1141 catggctacg cctttcccga cccagccata cccggcgacg acgacgacct tgccggcaat
     1201 gttcatatta gtcgtccgca ggatgccgtc ccacgcggat tgtcctgtgc cgaaacggtt
     1261 gtcgaagagg tgtttgcaga ggccctcgtt gactccgatg acagggaagg agagcttgcc
     1321 ggcgcgcgcg agcgcgcggt cacgaatgac tccggtcgtc gtttcttcac acgctcccac
     1381 gatagcgggc aggagttctg gacgctcgaa gtgaacggcg ttcacgaggt caccaccgtc
     1441 atcgatcagc agattgggcc ttgtggccag cacgttgccg aagtactcct tgtactcggc
     1501 gggcgtttcg ccgcgtttgg caaagaccgt gactcctcgc tctgcgagtg cctgggcgac
     1561 gtcgtcctgt gttgtaatgg ggttggacgc cgtgatggcg accttggctc cgccgcgcag
     1621 catcacgcag gccaggttgg ccgtctttgc ttcgaggtgg agcgcgatga cgacattgag
     1681 cccggcaaag ggctgttcct tctcaaaacg ctcggcaacc gacgagagga tcggcatgtg
     1741 gcggattgcc cactgcatct tctgttcacc tgactgactg ctcatgaacc acctccaggg
     1801 gagtagtaaa agagccgccg gaggacgagg tcctcgcggc ggcccgggaa agaaccggct
     1861 agcgctgttc tttgtagtag ggaaggccgt ctgccgcggg ggcgatggac ttgccgacga
     1921 aacctgccag gacggctagc gtaaggacat aggggagaat gagcgtaagg ccgggcgcca
     1981 gcttgggcgc gattccggtg acggcgatga tggactgcac gatctgacca gtgccaaaga
     2041 ggagtgcagc gccaagagcg ccgaaaggcg tccatttgcc gaaaatcatg gctgccatgc
     2101 caatgaagcc cacaccgttg ggcatggagt cgctgaaccc cgagaacgaa ctgatggaga
     2161 gtgccgcgcc accgatgcct gccagcgcag cccctgcgat gactgcgaac cagcgaacct
     2221 tgaacacatt gattccaagt gttgtcgcgg ccttggggtt ctcgccgcac gtacggatgc
     2281 gaagaccgag gaccgtgtgg tacagcagca cgtgcaagag gatcgtgcag atgagggcaa
     2341 gccatatcat gaatgacagg ttggagaagg ccccgagcag gggaatcttg gcgaggaacg
     2401 gtagatgcac acggtatgcc tgagccccaa agtagttctg gagttgcggc gagtacccgg
     2461 tcgtgctgaa gacgaattcc agcagatatg tcatgaggcc gacagcgatg acgttgatgg
     2521 ccgtgccggc gacagtttga tctccgttcc acctgatggt gataaaggcg tgaagcgctc
     2581 cgaccaggca gccgatggca atgccgaata gcaggccgat ccacggggaa tgcgagaaga
     2641 atgcgccaag caggccaaag aatgcgccga aacgcatgat accgtccaga ccaatgttga
     2701 ctacgcccgc attctcactg agcacaccgc acagcgcggc aaacaggatc ggagtagtgt
     2761 agtccagcat ctgtgcaatg gtgaggttga tctgtgtgat gttcatgctg cggcctcccg
     2821 gtcggctgat gggttcctgc ggctgcggcg gtgctggagg atcttgacca ggtagtatcg
     2881 cacggcaacc tgcagggcga tgaagccgac ggccatgccc tcgatgatgt tggagaatgt
     2941 cttggggatg ttggcgactt gcatggaggt agctccagtg cgcagggcac cgatgaacaa
     3001 tcccgccagc acgacgccca gaggttcgct ctggccaatc aacgcgacgc cgatacctgt
     3061 ccatccatac cccatggttg ctgtgttggg gaactggtac tgcatgccca ggatgaagaa
     3121 acagccaccg agtgccgcca gggcgccgga gaagaacatg agctgcatga tgcgccggtt
     3181 gacgt
//
LOCUS       DATMZD010000244         7859 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_26933,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000244 DATMZD010000000
VERSION     DATMZD010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7859
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26933"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1028
                     /locus_tag="VN478_05065"
     CDS             <1..1028
                     /locus_tag="VN478_05065"
                     /inference="COORDINATES: protein motif:HMM:NF018805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HWQ22076.1"
                     /translation="PIELYELMVRGSSRRSLTPVAFTARDHFPHRAAHVEWWYFTSVL
                     TTPARKAPIGLEVTFFKVRTLVDSVIVHAAVTDVDGRRFSFTGMVLPMVLRFAPDAHT
                     ILGVFGNSMRFNAESNNLLVDARLRGLRVNISCHVEDVMAQGEDGILTMQNSPGDESC
                     YFTMPNMTTEGTIVLKGESLPVTGHTWHDHQWGNFNLVGLKWDWFSLRFDTDDLYVML
                     FNFDRWGTTYGVGNLRSGGTTMRVDRFEVVTSDVCRTRTGGLYPIDWEVRLFDGPDAS
                     APFMIAHVRPVLNDQSVSSLITPDYWEGLCVVRARIMAPFELGGGNALEPKTLDGFAY
                     VELTGYE"
     gene            complement(1171..3168)
                     /locus_tag="VN478_05070"
     CDS             complement(1171..3168)
                     /locus_tag="VN478_05070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HWQ22077.1"
                     /translation="MTTRLTISPLKSGKLLPLIIAILAAAALALSVVSVSPGLVVLFQ
                     SLLHFPVILACAYYPKRGFVFSVVLACCYFLLMFSLSRDPATLGGAAVRVAFFVLVGW
                     VVAYLSIIRIRAQDRLAESKDLDHEVVENTPNLIVICGSDRKIRFANPAAGTAFGYST
                     AEMTGTDIMAYVSESERTRVTTAMQEWLSSGSKREPLEMNLVTRGGQPLPVISRGASV
                     CFAGEPAVLVLFTDISERKLAEEKLRAAHLQWNQFLDVSPDAMWIKDASGRYVAVNKT
                     YGLIDPSVKGSVIGKTDAEVFPPEKAEVYVANDRIAIEQGMCEEEFTATGVDGKLRSF
                     LTKKVVLRAADGSVAGTLGVSRDITDRKRSEEAIYNERLLLRTLIDNIPDSVYAKDLS
                     CRKTLANAAEVRYLGVKSEAEALGTNDFDSYPEETARRFFDDDKHVMETGEPVLNREE
                     YLTVADGRQRCLLTTKIPLRNLEGKIIGLVGIGRDITERKQMETQLEEMATHDFLTGL
                     PNRRLLLDRFEIAAALAHRNKTRLAVMSLDLDQFKEINDTYGHDSGDQVLKGVGKRLS
                     GSMRGSDTLARVGGDEFVALFLGEDVLTIAQRIVGSFVDPVTVADHQIQVSASVGIAV
                     YETDGEDMETLISKSDAAMYCAKEHGGSQFRFFADGDVKPD"
     gene            3559..3861
                     /locus_tag="VN478_05075"
     CDS             3559..3861
                     /locus_tag="VN478_05075"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWQ22078.1"
                     /translation="MKPNTKLDELTSFYKLLANESRLAILLALRDGEDNVAGICGQTG
                     LSQTLVSHQLAALHRSDVVRSRRDGRRTFYALADKHIDIIVGFALDHLAEREVHHE"
     gene            3854..4756
                     /locus_tag="VN478_05080"
     CDS             3854..4756
                     /locus_tag="VN478_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015849964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HWQ22079.1"
                     /translation="MSDHHDHELPTGRNLFISILMNVVITISEIVGGLVSGSLALLAD
                     AFHNATDTLAGVLAFWTLRLGRRDHDEQYTFGYRRAEILSAGINAGAMVVISLVLFRE
                     AYERFLHPVPIKGGIMLVVAVVGLVANLVSVLLLHRSASESLNVRGAYLHLLSDALSS
                     VGVVLGGIVVTLWHLAWVDPLVTVLVAGWVLREAFLVAREAGGILMERAPNGLELARL
                     EADICALPYVKNLHHVHVWQVSDTETMFEGHVNVDNISVSDTDPIRIQVQDLLRDKYG
                     ITHATLQFEYECCKGTGLIVHHHD"
     gene            4780..5760
                     /locus_tag="VN478_05085"
     CDS             4780..5760
                     /locus_tag="VN478_05085"
                     /inference="COORDINATES: protein motif:HMM:NF015318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HWQ22080.1"
                     /translation="MARRARNTQVVNCCLTVALAVLLLLPARAGAAQPMVDGVVRTGE
                     YDHSAVLGDGVYTLSWTVSGDIVWFGIRAMTTGWVALGLDPQIGMNRADMLFGWVSGG
                     KATVLDQYSTGTFGPHPDDTSLGGTMDILSSAGTEQAGTTTVELSRRLVTGDTHDKPV
                     PASGSLKLIWAIGNADGVSPHAAKGSAMIDVGAPTPVVLTFTIGSTVGTRNGARVTLD
                     AAPVIQNGRTLLPVRYVAEPLGATVAWAAGTRTVTIQRAGLTLALVVGKATAVVNGRI
                     LPIDAADSRIVPVILQGRTMLPARFVAEQLGCAVTYDPVTRVVTVTYPKP"
     gene            complement(5842..>7859)
                     /locus_tag="VN478_05090"
     CDS             complement(5842..>7859)
                     /locus_tag="VN478_05090"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWQ22081.1"
                     /translation="SDGKTARLVSAQGDSRAVQAQATSVPADEEPFSQVYRHAEPFFS
                     NDWHNPGSSDQSDDRSLALGVIPLVNTGHVVGSLNMAWYAPPDRGTDWKETLAAIARE
                     ITVGLDRIQTVTALQENAANLQAFFNSVQGFFFVVSRDGRILAVGEQGARRLGRTPAE
                     LVGTDINDLHPEEERDLSRVILGDMINGTRTSSTQTLVAADGTQIPVDTLVTRGSWNG
                     SLALFTTSQDVTGSRRAAEALAADRRRADALYEIIEAAGKARSTEDFLPRALAIALEA
                     TPYEGGGIYLVDGDRAVLSCAMGVSNELAELIRSVSLDEQPYRTVLREGRPVAFAEPG
                     MLPLDLSQEHGVASLVSVPIAVGDTIVGALNLVSSHVQSGELRTELLMSISRTIGDAV
                     VRLRADESARASREDFETLFEAIPRLVFILREDGTIARINKAAAERLGRTPDELSGVS
                     VLDLCPPDRCAEAACVVADMISGKTSLCTLSLMGREGDLFHVETQAVRTAWGGRPAIV
                     GVSHEVMSTGADEECAVQALLHDDLTRLYSASGFSAIASQQLKMAHRGHINAAMVIAE
                     LAGGVPEPHILTDFTNVLCDTFRDSDTIGRIGPARFAVLAIQTDNTCLDLLEQRLQES
                     VAAFNAERPASRPELVVRVVAIPVDAARLVFLDDLLSQAGAELSGPD"
BASE COUNT         1467 a         2423 c         2440 g         1529 t
ORIGIN      
        1 tgcccatcga gctgtatgag ctgatggttc ggggtagctc ccgccgctcg ctcacgcccg
       61 ttgcgttcac cgcccgggat cacttccctc accgcgcagc gcacgtcgaa tggtggtact
      121 tcacgagtgt gctgacgacg cccgcacgga aggcgcccat cgggctcgaa gtgaccttct
      181 tcaaggtgag gacgctggtc gacagcgtga tcgtccatgc tgccgtcacc gatgtggatg
      241 gccggagatt ctcgttcacc ggcatggtgc tgcccatggt cctccggttc gcgcccgacg
      301 cacacaccat cctcggggtt ttcggcaaca gcatgcggtt caacgcggag agcaacaatc
      361 tccttgtcga tgcacgcctg cgcggcctgc gggtgaacat ctcctgtcat gtggaggacg
      421 tcatggcaca gggagaggac ggtatcctga ccatgcagaa cagccccggc gacgaatcgt
      481 gctacttcac gatgcctaac atgaccaccg agggcacgat cgtcctgaag ggggagagtc
      541 tgccggtcac cgggcacacg tggcacgacc accagtgggg caacttcaac ctcgtcggcc
      601 tcaagtggga ctggttctca ttgaggttcg atacggacga tctgtacgtc atgctgttca
      661 actttgaccg gtggggcacg acgtacggtg tcgggaacct ccgcagtggg ggcacaacga
      721 tgcgcgtcga ccggttcgaa gtcgtgacat cagatgtgtg ccgcacccgg actggcggct
      781 tgtatcccat tgactgggag gtgcggctgt tcgacggtcc tgatgcatct gccccgttca
      841 tgatcgccca cgtccggccg gtactcaacg accagagcgt gtcatcgctg atcaccccgg
      901 actactggga ggggctgtgc gtcgtccgtg cgcggatcat ggcaccgttc gaactcggcg
      961 gcgggaacgc gctggagccg aaaacactgg acggcttcgc ctacgtggag ctgacaggat
     1021 acgaatgata gggcacggcc agtacaagaa ccgtggactc tcgccttcgc cgagtacgct
     1081 cctcagagac acccctcgta ttggactggt atgcttgggg gcgaatgaca catgaaggac
     1141 tgaggtgttt gcgtcgcgaa aggtggactg tcagtcgggc ttgacgtctc cgtccgcgaa
     1201 gaacctgaac tgactgccgc cgtgttcttt ggcgcaatac atcgccgcgt cggatttgct
     1261 gatgagggtt tccatgtcct ctccgtcggt ttcatagacg gcgatgccca cgctggcgga
     1321 cacctgtatc tgatggtcgg cgacggtcac cggatctacg aatgaaccca cgatcctctg
     1381 tgcgatagtg aggacatcct ctcccaggaa cagtgccacg aactcgtcgc cacccacacg
     1441 cgccagcgtg tcggatccgc gcatgctccc ggacaggcgc ttgcctaccc ccttgagtac
     1501 ctggtcgcct gagtcgtggc cataggtgtc gttgatctcc ttgaactggt ccaggtccag
     1561 agacatgacg gcaagcctgg tcttgttgcg atgcgccagt gctgccgcta tctcgaaccg
     1621 gtcgaggagc agcctccggt tgggaagtcc cgtgaggaag tcatgtgttg ccatctcctc
     1681 gagctgcgtc tccatctgct tgcgttcagt gatgtcacgg ccgatcccca cgagtcctat
     1741 gatctttcct tccaggttcc gaagcgggat cttggtggtg agcaggcaac gctgtcgccc
     1801 atctgcaact gtcaggtact cttctctgtt gagcacgggt tcgcccgttt ccatgacgtg
     1861 cttgtcgtcg tcgaagaacc tcctggccgt ctcttccggg tagctgtcga agtcgttcgt
     1921 accgagagcc tcagcttccg acttgacccc caggtaccgc acttctgcgg cgttggcgag
     1981 tgtcttccgg caggagaggt ccttggcata gaccgaatcg gggatgttgt cgatgagggt
     2041 gcgcaacagc agccgttcgt tgtagatcgc ctcctcggac ctcttgcggt ccgtgatgtc
     2101 gcggctcacc cccagcgtcc ctgccacgga gccgtccgcg gctcgcagga cgaccttctt
     2161 cgtgaggaag ctgcggagct tcccgtcgac gcctgtggcc gtgaactctt cctcacacat
     2221 gccttgttca atggcaatac ggtcgttggc gacgtagacc tctgccttct caggagggaa
     2281 cacctcagcg tcggtcttgc cgatgaccga gcccttgacg ctgggatcga tcaagccgta
     2341 cgtcttgttg actgcgacat accggccgga tgcgtccttg atccacatcg catccggaga
     2401 gacgtccagg aactggttcc actgcaggtg ggcggcacga agcttctctt cggcaagttt
     2461 gcgctcggaa atgtcggtga agagaacgag gactgcgggc tcacctgcga agcacaccga
     2521 agcgccccgc gagatgacag ggagaggctg tcctcccctt gtgacaaggt tcatctccag
     2581 gggttcccgt ttgcttcctg acgacagcca ttcctgcata gccgtcgtga cgcgtgtccg
     2641 ctcgctttcc gacacatacg ccatgatatc ggtccccgtc atctcggcag tggagtatcc
     2701 aaaggctgtg cccgccgccg ggttcgcgaa ccggatcttc cgatctgatc cgcagatgac
     2761 gatcaggttc ggcgtattct ccacgacttc atggtccaga tccttgctct cggccagcct
     2821 gtcctgcgca cggatcctga tgatcgacag atacgcgacg acccagccga cgaggacgaa
     2881 gaacgctacc cggacggcgg cccctcccag tgtcgccgga tcgcgtgaaa gagagaacat
     2941 caggaggaag tagcagcagg ccaggactac cgagaagacg aaacctcgtt tcgggtagta
     3001 tgcacacgcc aggatgacgg ggaagtgaag gagactctgg aacaggacaa ccagcccggg
     3061 ggagaccgag acgacgctca acgcgagagc agcggcggca aggattgcga tgatcagcgg
     3121 gaggagcttt ccgctcttca gcggagagat ggtgagtctg gtcgtcatga ggcgctccat
     3181 tccgtaagat ctggctccat ccgagacact cctcccggca tctgcccctc aaacaccgcg
     3241 ctcctgcgag agtgtcgacg ggaccctgtc cagcaggagt gaccgcacaa ccaaacttca
     3301 acctactata cgcttccgcc aggcagggtc aagcgggaaa ccggacaaac gcacgcaatc
     3361 ggacatcggt gtcgctgtgc tgttcagtgg cgcgccagcc agggtccccc aggattccca
     3421 gcggggtgga gaagaggcgc tactggttga catcgcctca gttgccgagc cccatggaga
     3481 gctgccggac gctccaccta caagaacgat gcagacaacg cttgacacat gatgactctg
     3541 tcatatgatg tgaacatcat gaaaccaaac acgaagctcg acgaactgac atcgttctac
     3601 aaactgctgg caaatgagtc acggctggcc attctgcttg cgctccgcga cggcgaagac
     3661 aacgttgccg gcatctgcgg ccagacgggc ctgagccaga cgctggtctc acaccagctg
     3721 gcggcgctgc accgcagcga cgtggtccgc tcgcggagag acgggcgccg cacgttctat
     3781 gcccttgcag acaagcacat cgacatcatc gtcgggtttg cgctcgacca tcttgcggaa
     3841 agggaagtgc accatgagtg accaccacga ccacgaactg cccacggggc gcaacctgtt
     3901 catttccatc ctcatgaacg tcgtcattac gatctccgag atcgtcggcg gcctcgtctc
     3961 cggctcgctg gcactgcttg ctgatgcgtt tcacaacgcg acagacacac tggcgggcgt
     4021 cctggccttt tggacacttc gtctgggacg gcgggatcac gacgaacaat acacgttcgg
     4081 ctaccgccgc gccgagatcc tgtcggccgg catcaatgcc ggggcgatgg ttgtcatctc
     4141 gctcgtcttg ttccgtgagg cctatgagcg gttcctgcat ccggtcccga tcaagggcgg
     4201 catcatgctg gtcgtcgcag tcgtcgggct ggtggcgaac ctcgtgtctg ttctcctgct
     4261 ccaccgctcg gccagcgaaa gcctcaacgt tcggggtgcc tacctgcatc tgttgtcgga
     4321 cgcgctttcg tccgtcggcg tcgttctggg gggcatcgtt gtgacgctgt ggcacctggc
     4381 gtgggtggat ccgctggtaa cggtcctcgt ggcagggtgg gtcctgaggg aggcgttctt
     4441 agttgcccgt gaggccggcg gcatcctcat ggaacgtgca ccgaatggac tggagctggc
     4501 acggctggag gcagacatct gcgcgctgcc atatgtcaag aacctgcacc atgtccatgt
     4561 ctggcaggtt agcgataccg agacgatgtt cgaggggcat gtcaacgtgg acaacatttc
     4621 agtcagtgat accgatccga tccgcatcca ggtccaggac ctgctgcgcg acaaatacgg
     4681 catcacgcac gcgaccctgc agttcgagta cgagtgctgc aaagggaccg gactcatcgt
     4741 tcaccaccac gactgatctc tagcaccggg aggcgtcgta tggctcgacg ggcacgcaat
     4801 acacaggtcg tcaattgttg tctgacagtg gctcttgcag tgctgctcct gctgccggcg
     4861 agagcggggg cggctcagcc gatggtcgac ggcgtcgtca ggacgggcga gtatgaccac
     4921 agtgccgtgc tgggcgacgg ggtctacacc ttgtcctgga ctgtttccgg cgatattgtc
     4981 tggttcggca tccgggcgat gacgacggga tgggtggcgc tgggcctcga cccccagatc
     5041 gggatgaaca gggcggacat gctcttcggc tgggtcagcg ggggcaaagc aacggtcctg
     5101 gatcagtact cgacggggac gttcgggcct catcctgacg acacgtcgct gggcggcacc
     5161 atggacatcc tgtccagcgc aggaaccgaa caggcaggga cgacgaccgt cgagctgagc
     5221 cggagactgg tgacgggcga tacacatgac aagccggtgc cggcgtcggg gtctctaaag
     5281 ctcatctggg caataggcaa tgcagacggc gtgtcccccc acgcggctaa ggggagcgcg
     5341 atgatcgatg tcggtgctcc gacgcccgtc gtgctgacgt tcaccatcgg ttcgaccgtt
     5401 gggacgcgaa acggtgcccg tgtgacgctc gacgctgctc ctgtcattca gaatggcaga
     5461 acactgctgc cggtgcgcta tgttgctgaa ccactcgggg cgacggtcgc gtgggcggcc
     5521 gggaccagga cggtgaccat ccagcgagct ggcctcacgc tcgcgctggt cgtaggcaag
     5581 gcgacggcag tcgtcaatgg acggatactg ccaatcgacg ctgctgacag ccgtatcgtt
     5641 cccgtcatcc tgcagggcag gacgatgctg cctgcgaggt tcgttgcaga gcagctggga
     5701 tgcgcggtca cctatgaccc tgtgaccagg gtcgtgaccg taacgtaccc caaaccctga
     5761 tccaggtatc tgcaggagcc cgagcgatca gggatccgcc tggagacgcg agcaaaggcc
     5821 gtcgtctgtg agcagtccct gtcagtctgg cccgctcagt tctgcgcccg cctgtgacag
     5881 aaggtcgtca aggaacacga gcctcgcggc atcgaccggg atggccacta cccgcacaac
     5941 aagctcaggc cggctggccg gccgctccgc gttgaacgcg gcgaccgact cctgcagacg
     6001 ctgttcgagg agatcaagac acgtattgtc cgtctggatg gcgaggacgg caaaacgggc
     6061 cgggccgata cgcccgatcg tatcagagtc gcggaacgtg tcacagagga cattcgtgaa
     6121 gtcagtgagg atatgaggtt ccgggactcc gccggccagt tccgcgatga ccattgccgc
     6181 gttgatgtgt ccacgatgcg ccatcttgag ctgctgactg gcgatggcag agaaacccga
     6241 tgcgctgtac aggcgagtca agtcgtcatg gaggagcgcc tgcacggcgc attcctcgtc
     6301 cgctccagtc gacatcactt cgtggctcac gccgacaatg gctggcctgc cgccccatgc
     6361 cgtgcggacg gcctgagtct ccacgtggaa gagatctcct tcgcggccca tgagcgaaag
     6421 tgtacagagg cttgtcttgc cgctgatcat gtcagccaca acacaggcag cctctgcaca
     6481 tcggtccgga ggacagaggt ccagcaccga cactccgctc aactcgtctg gtgtccgccc
     6541 cagcctctct gccgccgcct tgttgattct ggcgatcgtt ccatcctcgc gcaggatgaa
     6601 cacaagccgt gggatagcct cgaacaacgt ctcgaagtcc tcccggctgg cccgcgcaga
     6661 ctcgtcggca cggagacgaa cgacagcgtc gccaatcgtg cggctgatcg acatcagcag
     6721 ttcagtccgg agttcgccac tctgaacgtg cgagctgacg aggttcagag cgccgacgat
     6781 ggtgtccccc accgcaatcg gtacggacac cagtgaagcg acaccatgtt cctgggacag
     6841 gtcgagcggc agcatgccgg gttcggcgaa tgccaccggt cttccttccc tgaggaccgt
     6901 gcgatagggc tgctcgtcca gggagacaga acggatcagc tcggcgagtt cattgctgac
     6961 acccatcgca caggacagca cggcgcggtc gccatcgacg aggtagatgc caccgccttc
     7021 gtagggagtg gcctcgaggg cgatagccag cgccctgggc aggaagtcct ccgtcgagcg
     7081 agccttgcct gctgcctcga tgatctcgta aagagcgtct gcccgccttc tatcagccgc
     7141 aagcgcctca gcagccctcc tcgagccggt gacatcctga ctcgtcgtga acagcgcgag
     7201 gctgccattc catgagccac gcgtcacgag ggtgtcgacg gggatctgtg tgccatccgc
     7261 ggccacaagc gtctgcgtac tgctcgttcg cgtgccgttg atcatgtcgc ccaggatgac
     7321 gcgtgacaaa tcgcgttctt cctcggggtg gaggtcgttt atgtctgttc cgaccagttc
     7381 tgccggcgtt cgtccgagtc gacgcgcccc ctgttcaccg actgccagga tcctgccgtc
     7441 gcggctgacg acgaagaaga atccctgcac agagttgaag aacgcctgga ggttcgcggc
     7501 gttctcctgc aatgctgtca ctgtctggat gcggtccagg ccgacggtga tctcgcgggc
     7561 gattgccgcc aaagtctcct tccaatctgt cccgcggtca ggcggcgcat accatgccat
     7621 gttgagcgaa cctacgacgt gaccagtgtt gacgagaggg atgacgccga gtgccagaga
     7681 tcgatcgtca gactggtccg aagatcccgg gttgtgccag tcgttgctga agaagggttc
     7741 ggcatgcctg tacacctggg agaacggctc ctcgtcggcg gggaccgacg ttgcctgggc
     7801 ctgcaccgcc cgggagtccc cctgcgccga caccaggcgc gccgtcttcc catcggagt
//
LOCUS       DATMZD010000245         2084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_117247,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000245 DATMZD010000000
VERSION     DATMZD010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117247"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VN478_05095"
     CDS             complement(<1..342)
                     /locus_tag="VN478_05095"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWQ22082.1"
                     /translation="MIRLFKSARILDTGRGSDDATRVVDILVDGMRIGRIADHVDPPE
                     GASIVDASKCLVTPGFVNTHGHLAMTLLRGFADEVPLDAWLQSYMFPREALMNGDDAY
                     YGTLLALAEGVM"
     gene            complement(452..1378)
                     /locus_tag="VN478_05100"
     CDS             complement(452..1378)
                     /locus_tag="VN478_05100"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HWQ22083.1"
                     /translation="MAWQWYHDTFEPGEVHLHALTGVIATKRTKYQTVEIVESEHYGK
                     MLILDGDCQSSVKDEYIYHECLNEPAMVCHPDPRRVLVVGGGEGASLRELLRFKSVEH
                     IDMCDIDEEANQMYEQYLPEYHQGTFHDPKVSMHFEDARKFIERQPDHSYSVIIEDLT
                     DLFEGGPSIVLFTREFYQHAFRVLDEGGTLCVTSSYLKPTNVAVHKRILDTIRSVFPI
                     ARSLYSYVGAYDVPWSYILASKKNDPLEMDAATVDRILADRVGDTSALRFYDGVTHER
                     IFRMPKDIRTMLALPGEPLEDGKPFGIVPKGI"
     gene            complement(1356..1781)
                     /gene="speD"
                     /locus_tag="VN478_05105"
     CDS             complement(1356..1781)
                     /gene="speD"
                     /locus_tag="VN478_05105"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HWQ22084.1"
                     /translation="MRTEGRHMVVEVSGCNPDVLNDLDRVKEILRESALQANAEILEL
                     AFHHFTPQGVSGVVVISESHLSIHTWPEYGYAALDVYTCGEKTDPWRAMEYAAEKFEA
                     TTIVKTEIARGQEIEGRPGVYGHSVVESEVMRDGVAVVS"
BASE COUNT          474 a          630 c          547 g          433 t
ORIGIN      
        1 catgacgcct tcggccagtg cgagcagggt accatagtag gcgtcgtcgc cattcatgag
       61 agcttcccga gggaacatgt aactctgcag ccacgcatcg agaggcactt cgtcggcgaa
      121 cccccgcagg agcgtcatgg ccaggtgccc atgcgtgttg acaaacccag gagtgacaag
      181 gcacttcgac gcatcgacaa tactggcgcc ctccggcgga tccacgtgat cagcgatgcg
      241 gccgatcctc atcccgtcga caaggatgtc cacgacgcgt gtcgcgtcgt cgcttccccg
      301 cccagtgtca agtattctgg cagacttgaa caatcgtatc atcacccacg ctccttttcc
      361 catatcatag caaatctcat gcaaatctcg ctagcgaaaa gcccgcctct gtcgaggcgg
      421 gctggatcat gcgtcgtacg gaccagcagc tctagatgcc tttggggacg atgccgaacg
      481 gcttgccgtc ttcgagaggc tcaccgggca gagccagcat tgtccgaatg tccttgggca
      541 tgcgaaagat acgttcatgg gtcaccccat cgtagaacct cagggcactg gtgtccccga
      601 cgcggtcagc caggatccta tcgaccgtcg cagcatccat ttccaaggga tcgttcttct
      661 tggaggcaag gatgtacgac cacggaacat cgtacgcgcc gacataggag tagagcgaac
      721 gggcgatcgg gaagacggaa cggatggtat cgagaatcct cttgtggact gcaacattcg
      781 tgggtttcag atatgatgac gtaacacaga gagtgccacc ttcatccaga acgcggaagg
      841 cgtgctgata gaactccctg gtaaacagga caatggaagg tccaccctcg aagagatcag
      901 tcaggtcctc gatgatcacg ctgtaggaat gatcaggctg ccgctcaatg aacttgcggg
      961 cgtcctcgaa gtgcatgctg accttggggt cgtgaaaggt accttggtga tactccggca
     1021 ggtactgctc gtacatctga ttggcttcct cgtcaatgtc gcacatgtcg atgtgctcca
     1081 ccgacttgaa gcgcagcaac tcgcgaagag acgcaccttc accgcctccg actaccagca
     1141 ccctgcgcgg atccggatga cataccatgg ccggttcgtt caggcactcg tgatagatgt
     1201 actcgtcctt caccgaactc tggcagtcgc cgtccaggat gagcatcttc ccgtagtgct
     1261 cagactctac gatttcaacg gtctgatact tcgtcctctt tgtcgcgatg acacctgtga
     1321 gcgcgtggag gtgaacctcc ccgggctcaa acgtgtcatg ataccactgc cacgccatct
     1381 ctcatgacct ctgactcgac gaccgagtgt ccatagacgc ctggacggcc ctcgatctcc
     1441 tgcccacgag caatctccgt cttgacgatt gtcgttgctt cgaatttctc agcggcgtac
     1501 tccatggccc gccatggatc ggtcttttct ccacaggtat aaacgtctag ggcagcgtat
     1561 ccgtactcag gccatgtgtg aatggacaga tgcgactcgg agatcactac taccccacta
     1621 accccctgag gagtaaaatg gtgaaacgcc aattccagga tctctgcatt tgcctgcaga
     1681 gcagactcgc gcaagatctc cttaactctg tccagatcgt tcaaaacatc cggattacac
     1741 ccagagacct cgactaccat gtgtctacct tcggtgcgca tctcgaccac cacccccttt
     1801 caaagattag taaagtaagg atgaggacat tactttaacg tgtagcatca taccagcaat
     1861 cgacaggatt gcaatgagga tgagcctcac gcttaggtgt ccgaaggaag aggccactcg
     1921 acccgggagc gcagtcagca agacttggct actgcagggt gggcaagcct gagttcgcca
     1981 gtcgctctca acgagccgaa aacacggccc gcgactcgca accgtgggga aaaccgtacc
     2041 ctcgtccctg ccctgcccag ggccctggca cacgaacccc gtac
//
LOCUS       DATMZD010000246         2475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_117254,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000246 DATMZD010000000
VERSION     DATMZD010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117254"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1110
                     /locus_tag="VN478_05110"
     CDS             <1..1110
                     /locus_tag="VN478_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007076461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWQ22085.1"
                     /translation="VRDKVAEVINESGPVTGLFSEYELKLAKLVNRLVPSVEMLRMFG
                     SGTEAVMGAIRAARTHTGKRKIIKVGGAYHGWSDSMVYGLHVPGTGRFESRGIPTGAT
                     GNTEEVFPNDLGGLRRLMIRNRFRGGTAAVIVEPVGPESGTRPVYRDYNQKVRELCDE
                     FGALLIFDEVVTGFRLGMGCAQGYFGVKPDLTVFGKCITGGYPMAGGLGGRRDVMMHL
                     SAGIGGTGERAYVGGTLTANPLSCAAGYFALMEMERTNAPVIAGRAGDRLVKGLQEII
                     DRRRLPFVAYNQGSIVHLETAGVMLLDFRHPLKLAKELKPRNHMMQEMGAAYMSQGII
                     TLAGSRMYTSMADTDEIIDDALRRFDVVLGSVEGA"
     gene            complement(1179..>2475)
                     /locus_tag="VN478_05115"
     CDS             complement(1179..>2475)
                     /locus_tag="VN478_05115"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016549063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HWQ22086.1"
                     /translation="GRQLMNCVLWMDMRGADNLRKITRGSVNVSGYDPVKLMRWIKLT
                     GGAPALSGKDPSAHMLFIRDVFPDVYAKTYKFLNVPDYMNMRLCGRIAATPDSILTSW
                     VTDNRDSAHVAYSPALIRNSGIDAGKFPEIVACDSVLGEVRSDVAHELGLKPGVRVVA
                     GSIDTTSAAVGSGAVRDYEAHLYIGTSSWIAAHVPFMKTDVASSIASVPCAIPGKYLM
                     TILQTTAGGNLAFLRDKVLYHKDKLLREEQAPDVYQIMDRIAEQVPAGSNGVIYAPWI
                     YGERSPVEDRHVRASIFNLSLENNREDIIRAFLEGVALNTRWMLGPGEKFLGRKLESL
                     NIVGGGAKSDVWCQIFADVLNLTIRQVADPIGVNARGAAFIAAEALGYMKFEDVARYT
                     TIAREYTPLSEHRAVYDKTFKEFVRYYRQTREIYRELNP"
BASE COUNT          490 a          752 c          771 g          462 t
ORIGIN      
        1 gtgcgcgaca aggtggccga ggtcatcaac gagtcgggac ccgtcaccgg cctgttcagc
       61 gagtatgagc tgaagcttgc caagctcgtc aaccgtcttg tccccagtgt cgagatgctg
      121 cgcatgttcg ggtcggggac cgaggcagtc atgggcgcca tccgtgcggc gcgcacacac
      181 acgggcaagc gcaagatcat caaggtcggg ggagcctatc acggctggag cgattcgatg
      241 gtctacggcc tgcacgtacc gggaactgga cgtttcgaat ccaggggaat accgactggc
      301 gcgaccggca acaccgagga ggtctttccc aacgaccttg gcggcctgcg gcgcctgatg
      361 atccgcaacc gcttccgagg cggcacggcg gccgtcattg tcgaaccggt cggccccgag
      421 agcgggacac gtccggtgta ccgggactac aaccagaagg tgcgcgaact ctgcgacgaa
      481 ttcggcgcgc tcctcatctt cgacgaagta gtgaccgggt tccgcctggg catggggtgc
      541 gcacagggat acttcggcgt caagccggac ctcacggtct tcggcaagtg catcaccggc
      601 gggtacccaa tggccggagg actcggcgga cggcgcgacg tcatgatgca tctctcggcc
      661 gggatcggcg gcacgggaga acgggcatat gtcggcggga cgctgacggc caatccactg
      721 tcatgcgcag ccggctattt cgcgctgatg gaaatggaac ggaccaatgc gccggtcatc
      781 gccgggcgcg cgggggaccg tctggtgaag ggactgcagg agatcatcga ccggcgcaga
      841 ctgccgttcg tcgcgtacaa tcagggttcc atcgtgcacc tcgagacggc gggcgtcatg
      901 ctgctggact tccgccatcc gctgaagctg gcgaaggaac tcaaaccccg gaaccacatg
      961 atgcaggaga tgggcgccgc ctacatgagc cagggcatca tcacgctggc cggcagccgc
     1021 atgtacacaa gcatggccga taccgacgag atcatcgacg atgcgctccg gcgcttcgac
     1081 gtcgtcctcg ggagtgttga aggagcctga agcatagctg catgacaaag gggtggacca
     1141 gagaaggccc atccctttct tcccttcggg agtctgccct atgggttcaa ctcgcggtag
     1201 atctccctgg tctggcgata gtaccggaca aattccttga aggtcttgtc atagaccgcc
     1261 cggtgttccg acaaaggcgt gtattccctg gcgatggtcg tgtatcgtgc cacgtcctcg
     1321 aatttcatgt atcccagcgc ttcggctgcg ataaacgctg ccccacgcgc gttcaccccg
     1381 atcgggtctg ccacctggcg aatggtgagg ttcaggacgt cggcgaagat ctggcaccag
     1441 acgtcggatt tggcacctcc accgacgatg ttgagagact ccagcttgcg tccgaggaac
     1501 ttctcacccg gtccaagcat ccaccgcgtg ttgagggcga caccctcgag aaatgctcgg
     1561 ataatgtcct cccggttgtt ctccagagac aggttgaaga tggatgcgcg tacgtgacgg
     1621 tcttcgacgg gggaccgttc gccgtagatc catggcgcgt agatgacgcc gttgctcccg
     1681 gccggcacct gctcggcaat gcgatccatg atctggtaga cgtcaggcgc ctgctcttca
     1741 cgaagcagct tgtccttgtg atagaggacc ttgtcgcgaa ggaaggccag gttcccccct
     1801 gcggtcgtct gcaggatcgt catcaagtac ttgccaggaa tggcacatgg aaccgaggcg
     1861 atcgagctgg ccacgtccgt cttcatgaag ggaacgtgtg cggctatcca ggacgacgta
     1921 ccgatgtaca gatgtgcctc gtagtcacgg acggcacccg atccgacagc agccgacgtc
     1981 gtatcgatgg accccgccac cacccgaacc ccgggcttga gccccagctc gtgcgcgacg
     2041 tctgatctca cttcccccag cacggagtca caggccacga tctcagggaa cttgccagcg
     2101 tcgattcccg agttccgtat gagcgccggc gagtaggcga catgtgcgga atcgcgattg
     2161 tccgtgaccc aggaagtcag aatggaatcc ggcgttgcgg caatccgtcc acaaaggcgc
     2221 atgttcatgt agtccgggac attcaggaac ttgtacgtct ttgcatacac gtccgggaac
     2281 acgtcgcgaa tgaacagcat gtgtgctgac gggtccttgc cggacagcgc aggagctccg
     2341 cccgtcaact tgatccagcg catcaacttg acggggtcat atccagagac attcactgac
     2401 ccccgcgtga ttttcctgag gttgtcagcg ccacgcatgt ccatccagag cacgcagttc
     2461 atgagctgcc tgccg
//
LOCUS       DATMZD010000247         6790 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_17584,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000247 DATMZD010000000
VERSION     DATMZD010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6790
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17584"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1500)
                     /locus_tag="VN478_05120"
     CDS             complement(<1..1500)
                     /locus_tag="VN478_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWQ22087.1"
                     /translation="MPEELDQDLLSIQEARNLAQQAQVAQEAFVHFNQYQVDVVCASM
                     AEAAYEAAERLGRMAVEETHMGVAEHKTIKNQFASKNVWMSIKDVRTEGVIRRDEAKK
                     IIEIAWPFGVIAAMVPTTNPTSTAIFNAITAVKARNAIIILPHPSAPKCTAEAVHVLE
                     QAAEAAGAPKGLVSCMTHISMGGTSELLKRPEIKLIVATGGEAMVSYAHSVGKPALGV
                     GPGNVPVYVDRSADVEKAAHDIVTSVNFDYAVICAHENAVIADKPIADQLAKAMERNG
                     AYFLSDEQADKLGKAMIRPNGLMQADFVGRSPQMLAEQAGFKVPDEALILVARLSAVG
                     PKIPLSREKLAPVLAFYVEDGWHTGCARCIDTLEFGGKGHTMGLHCTDEDIIMQFGLQ
                     KPAFRIIVNAGTTAAAIGYTTDLMPSLTLATGGMGGGVSSDNITVYHLFNVKRVAYGL
                     RPFDPPVVYDRRSTAAPAAAPAAAPAGKQSPSEIEDLVRKVIREELSRKG"
     gene            complement(1590..2261)
                     /gene="eutL"
                     /locus_tag="VN478_05125"
     CDS             complement(1590..2261)
                     /gene="eutL"
                     /locus_tag="VN478_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HWQ22088.1"
                     /translation="MALEMERNLKPEILSVRLIPNVHPDMAKSLELLPGEKSLALFTC
                     NIDDPLYIGGDEATKKAEVRVAYCRSHFAGGSYPSGPLSGEAMLLLAAPNPAEATAGI
                     NAVLEFVDALRYSTVTLPVQLAPTLVWLAYTISRSGTYLSKEAGVEVGTPIAYITAPP
                     LEGHYAMERSLKAADVAVGSFTRLSETNYHGALLYGTQSACRAACRAFDEAVLSVARD
                     PLAFQ"
     gene            complement(2415..3281)
                     /locus_tag="VN478_05130"
     CDS             complement(2415..3281)
                     /locus_tag="VN478_05130"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF017768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase light chain EutC"
                     /protein_id="HWQ22089.1"
                     /translation="MAAYELYKGTQKQADIEARRAEAAKFGVEVEDVTTPEKRHTVGV
                     DHPVDAAGCEALIHATDQRVCVGRNGPRPKLLTYFQMRADHGATQDSLKYSVPEEKIE
                     ALGLLHVGETQTNGLLETYLRLPPLGRKLSDEAKAVVLEKCVKNPTVQFVVSNGLSGY
                     AIERYAGDVLKSMTDGLKAAGITVGTSVYVNRARVGVMNDFGTLLNADVVVVLIGERP
                     GLIISDALSIYMGYKPRWEPLTTDGNRDNICMISKKGKNPIEAAAEAVALVKDYLKYK
                     TSGVELATAKRA"
     gene            complement(3297..4664)
                     /locus_tag="VN478_05135"
     CDS             complement(3297..4664)
                     /locus_tag="VN478_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HWQ22090.1"
                     /translation="MAFRTKLFGITYDFKDVKEVLAKANEQKSGDEAAGVAAKSVAER
                     IAAKTVLAEIPMWVLRAQPVVPYEDDEITRAIDDGLDLVAYEKIKNKTVGEMRAWILD
                     ENTTSEDIAAISKGLTGEMAAAVAKLCSSLDLIVGGKKMPCVVHNVCTMGLPGRMGAR
                     LQPNHPTDDLDAIRAEIYDGLAFGIGDQVIGINPAIDQVENLMKMYDMMWDIKEKWSI
                     PASNCILGHVTTQMECLKKGAKVGQVFQSIAGTQAALESFGVEVGMLDEANELAKKYC
                     YSEGPNYMYFETGEGTELSAYANNDADQLTLEARKHALARRYSPIGVNSVVGFIGPEY
                     LYNSDEIMRAGLEDHFCGKLLGIPLGLDNCYTNHVRADQNTNEFLMVLDAVAGCNYHM
                     GVSMGDDVMLSYQCASYHDTAALWSAMGTRPAPEWEKWAEELGLLKNGKLTDKAGDPS
                     FFLKR"
     gene            complement(4677..6146)
                     /locus_tag="VN478_05140"
     CDS             complement(4677..6146)
                     /locus_tag="VN478_05140"
                     /inference="COORDINATES: protein motif:HMM:NF018032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HWQ22091.1"
                     /translation="MASVELISVGIDIGTTTTQIVISKLLVAETATLSKMPQARYGPA
                     RKPRADVAKKDVLYQSPVHFTPLDHDGLVDVDELEKILWADYKEAGFTPDQIDTGAVI
                     ITGESAKSQNAERVLDIVAPLAGDFVSTVAGPSLESHLAGRGSGAAAWTSEHFAWGTN
                     VDIGGGTTNVAVFHQGDLMDTVVLSVGGRHIQIDQKSGVVTHVTRSGQKMLNHLGIDL
                     KVGDTATVEVLRKVARLMSDLIVEALSGQPSQTARDLMETAPLSHPVTDMTIFFSGGV
                     ADFYYDTKPVTTIQDVAVYGDIGPLMGEELRNHPRVKEWSVLKPLHTERATVMGASHE
                     TITLSGMTIWVAPKELPMRNVPVVRPIFDGIPDEQTLARAVLDGYGRWDLDPAKDRVA
                     LSLDLSAVDCYDDLKKLARGIEMFASKYISPSIPLILVTERDLGKALGQVVSAGLPQF
                     KVLSIDEVTLAEGDYIDIGQSMLGDRLVSLSVKTLIFSM"
     gene            complement(6160..>6790)
                     /locus_tag="VN478_05145"
     CDS             complement(6160..>6790)
                     /locus_tag="VN478_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014739776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HWQ22092.1"
                     /translation="AATDAGATAAGRVGEMVSIHVIARPDKDIDLIIDNMSKNLGLPV
                     GEGASPAPSEASAHTPGTLSAEPSPVSGAVEPVVQPISHVSSQPVTATDAARVIAPAT
                     SIQEPSVVMPVIPVGLSGEPPGSVHATVARRLDLNTATAEQLDALPGVGPALAERIVE
                     FRAQQGPFSSVEELKKVQGAKKTLVTSLKEHLYVDKKNNPGKNSRKSEK"
BASE COUNT         1311 a         2032 c         1972 g         1475 t
ORIGIN      
        1 ccccttgcga gacagttctt cgcggatgac cttccgcacc aggtcttcga tctccgacgg
       61 cgactgcttg ccggccggag ctgccgcagg agctgctgct ggcgcagcgg tcgacctgcg
      121 gtcgtacaca acaggcgggt caaacggcct cagcccgtac gcgacacgtt tcacgttgaa
      181 caggtgatag accgtgatgt tgtcgctgct gacgcctcca cccataccac cggttgccag
      241 ggtcaacgag ggcatgaggt ccgtcgtata accgatagcc gcagcagtgg tccccgcatt
      301 gacaatgatt cggaacgcag gcttctggag cccgaactgc atgatgatat cctcgtcagt
      361 gcagtggagg cccatcgtgt gacccttccc gccaaactcc agcgtatcga tacaccgcgc
      421 acatcccgtg tgccatccgt cctcgacgta gaaagcaagg acaggtgcca gtttctcgcg
      481 tgagagaggg atcttgggac caacggcgga cagtctggcg accaggatga gcgcctcgtc
      541 aggaaccttg aatccggcct gttcggccag catctgcggc gagcgtccaa cgaagtcggc
      601 ctgcatgagt ccatttggac ggatcatggc tttacccagc ttgtcggcct gctcatccga
      661 caggaagtac gccccgtttc gctccatggc tttcgcaagc tggtcggcga tcggtttgtc
      721 agcaatgacg gcattctcat gagcacagat gaccgcgtag tcgaagttga cgctcgtgac
      781 aatgtcatga gccgccttct cgacgtccgc gctgcggtcg acgtacaccg ggacgtttcc
      841 aggaccgacg ccgagcgcag gcttgcctac gctgtgcgca taggagacca tggcttcgcc
      901 gccggttgca acgatcagct tgatctccgg ccgcttgagc agctcggaag tcccgcccat
      961 gctgatgtgc gtcatacagc tgacgagacc cttgggggct ccagcagcct ctgccgcctg
     1021 ttccagaacg tgaaccgcct cagcggtgca cttgggggct gaggggtgag gaaggataat
     1081 gatggcgttg cgcgccttga cggcggtaat cgcgttgaag attgccgtgg atgtcgggtt
     1141 ggtggtagga accatagcgg cgatgacgcc gaagggccag gcaatctcga tgatcttctt
     1201 ggcttcatca cgcctgataa cgccctcagt tcggacatcc ttgatggaca tccatacgtt
     1261 cttcgacgca aactggttct taatcgtctt gtgctcggcg acacccatgt gtgtctcttc
     1321 gacagccatc cgtccgagtc gctcggcggc ttcatatgca gcttcagcca tcgacgcgca
     1381 cacaacgtcc acctgatact ggttgaaatg aacgaacgct tcctgcgcga cctgagcctg
     1441 ctgtgccaaa ttccgagcct cttgaatcga cagcaggtct tggtcgagtt cttccggcat
     1501 gttctgcctc ctcttacaga tctccgcccc ggttcagatt ccaaccggag cgcccatcag
     1561 aaactaagtc gtgcgagtcg cagaacaccc tactggaaag ccagcggatc tcgtgctaca
     1621 gacaggactg cttcgtcgaa cgcgcggcaa gctgcacggc aagcactctg agtgccatac
     1681 aggagcgcac cgtggtagtt ggtctcgctg agcctggtga atgatccgac agccacgtca
     1741 gctgccttca gggaacgttc catggcatag tggccctcaa gaggcggcgc agtgatgtac
     1801 gcgataggcg tcccgacttc aaccccggct tccttggaca gatacgtccc agaacgcgag
     1861 atcgtgtagg ccagccagac aagcgttgga gcgagctgaa ccgggagtgt gaccgtcgag
     1921 taccttagcg cgtcgacgaa ttccagaaca gcgttgatgc ccgcagtagc ctcggcaggg
     1981 ttcggagcag ccaacaggag cattgcctcg cccgagagag gtcctgaggg ataggaacca
     2041 ccggcaaagt gcgaacggca gtaggccaca cgtacctcgg ccttcttggt ggcttcgtct
     2101 ccgccgatgt acagcggatc atcgatattg caggtgaaca gtgccaaact cttctcccca
     2161 gggagaagtt ccagagactt cgccatgtcg gggtgcacgt taggaatgag ccgcaccgac
     2221 aggatttctg gcttcaggtt ccgttccatc tctagagcca tgtcattcta ctcctttttg
     2281 atacctcgtt ctccggtttc ccaggacaac cggtcggctg gccgcggaac cgtcaacgga
     2341 ttccagcagc cggcccccag ggcagatccc aggatcgtgg ggtcaatccc tgaggtccgg
     2401 cgatcgctac tgtactaagc tctcttcgca gtggcgagtt cgacgccgct ggtcttgtac
     2461 ttcaggtagt ccttcacgag agcgacagcc tcggctgcag cctcgatcgg gttcttgccc
     2521 ttcttggaga tcatgcagat gttgtcacgg ttgccgtcgg tcgtgagcgg ttcccagcga
     2581 ggcttgtagc ccatgtagat ggacagggcg tcggagatga tgagacctgg acgctcgcca
     2641 atcaggacaa caacgacatc agcattcagg agggttccaa agtcattcat gacaccgaca
     2701 cgagcgcggt tgacatagac ggaagtcccg acagtgatgc cggcagcctt gaggccgtcg
     2761 gtcatgctct tgaggacgtc accggcatag cgctcgatgg catagccact gagaccgttg
     2821 ctgacgacaa actgaaccgt cgggttcttg acgcacttct cgagcacgac agcctttgcc
     2881 tcatcgctga gcttgcggcc aagcggtggc aggcggaggt aggtctccag cagtccgttg
     2941 gtctgggtct cgccgacatg caggaggccc agagcctcga tcttctcttc cgggacgctg
     3001 tacttcagtg agtcctgggt ggcaccatgg tcagctctca tctggaagta ggtgaggagc
     3061 ttcggacggg ggccgttgcg gccaacgcag acgcgctggt ctgtcgcgtg aatgagggcc
     3121 tcgcagccag cagcatccac aggatggtcg acaccgacgg tgtgacgctt ctcaggggtg
     3181 gtaacgtctt cgacttctac gccgaacttg gcagcctcgg cacgacgtgc ctcgatgtct
     3241 gcctgcttct gtgtaccctt atacaactcg tatgcagcca tttctactct cctctcctac
     3301 cgcttcagga agaacgacgg atcgccggcc ttgtcagtca gcttgccatt cttgagcagt
     3361 ccaagttcct cggcccactt ctcccattcg ggagctggac gagtgcccat ggcgctccag
     3421 agagcagctg tatcgtggta gctggcacac tgatagctca gcatgacatc gtcgcccatg
     3481 gaaacgccca tgtggtagtt gcaccctgca acagcatcga gtaccatcag gaactcgttg
     3541 gtgttctggt cggcacggac gtggttggtg tagcagttgt ccagcccgag cgggatgccc
     3601 agaagcttgc cgcagaagtg gtcttcgagg cctgcgcgca tgatctcgtc agagttgtac
     3661 aaatactccg gtccgatgaa accgacgacg gagttgacgc cgatggggct gtagcggcga
     3721 gcaagcgcgt gcttgcgggc ctcgagcgtc agctggtcag catcgttgtt cgcgtaggcg
     3781 gacagctcgg tgccttcacc ggtctcaaaa tacatgtagt tggggccttc agagtagcag
     3841 tacttctttg ccagctcatt ggcctcgtcc agcataccca cctcgacacc aaaagactcg
     3901 agggctgcct gggtccctgc gatgctctgg aacacctgac cgacctttgc gcccttcttc
     3961 aggcactcca tctgggtcgt cacgtgcccg agaatacagt tggatgcggg aatgctccac
     4021 ttctccttga tgtcccacat catgtcatac atcttcatga ggttctcaac ctggtcgatg
     4081 gctgggttga taccgatgac ctggtcaccg atgccaaatg cgagaccgtc gtagatctcg
     4141 gcgcggatgg cgtcgaggtc gtccgtagga tggttgggct gaagacgtgc acccattcgg
     4201 ccggggagac ccatggtgca cacgttgtgc acaacacatg gcatcttctt gccaccgacg
     4261 atcaggtcga ggctggagca gagtttcgca acagcggcag ccatttcacc ggtgaggccc
     4321 ttgctgattg cggcaatgtc ctcggacgtc gtgttctcgt caaggatcca agcccgcatt
     4381 tcacctacgg tcttgttctt gatcttctcg taggcaacga gatcgaggcc atcatcgatg
     4441 gcacgggtaa tctcatcgtc ttcgtaggga acaaccggct gggcacgcag aacccacatc
     4501 gggatctcgg ccagaacagt cttggctgcg atgcgctctg ctacgctctt tgcagcaacg
     4561 cccgctgctt cgtcaccgga tttctgttcg ttcgccttcg ccaggacttc cttgacgtcc
     4621 ttgaagtcgt acgtaatgcc gaacagtttg gtacggaatg ccatgtttcc tccttgtcac
     4681 attgagaaga taagtgtctt cacggaaaga gaaacaagac gatctccaag catcgattga
     4741 ccaatgtcga tatagtcccc ttctgccaat gtgacctcat cgatcgagag caccttgaac
     4801 tggggtagcc cggcacttac gacctgtcct aatgccttgc ccaggtcgcg ctctgtgacc
     4861 agaatcagcg gaatactcgg cgaaatgtac ttgctggcaa acatctcgat gccgcgcgcc
     4921 agcttcttca ggtcgtcata gcagtccact gcggacaagt cgagggagag agcgactcga
     4981 tcctttgcag gatcgaggtc ccagcgacca tatccgtcca aaaccgccct ggccagtgtc
     5041 tgttcgtcgg gaatgccgtc gaatatcggc cggacgacag ggacgttacg catgggcagc
     5101 tctttgggag caacccatat ggtcatgcca ctcaacgtga tcgtctcgtg gcttgcaccc
     5161 attaccgtgg cacgctcagt gtggagaggt ttgagaacgg accactcctt gacgcgtgga
     5221 tgattccgca attcctcgcc catgagggga ccgatgtcac catacacggc aacatcctgg
     5281 atagttgtga caggcttcgt gtcgtagtag aagtctgcca cgccgcccga aaagaagatc
     5341 gtcatatcgg tgaccgggtg tgacagcgga gccgtctcca tgagatcacg cgccgtctgc
     5401 gacggctgtc cggacaacgc ttccacgatg aggtcactca tgagccgggc aacctttcgc
     5461 agtacctcca ccgtggccgt atcaccgact ttgaggtcta tccccaggtg attcagcatc
     5521 ttctgcccgc tccgcgtgac atgcgtgacg acgccggact tctgatcgat ctggatgtgg
     5581 cgaccgccga cagacaggac gacggtgtcc atgagatccc cctggtggaa gaccgcaacg
     5641 tttgtcgtgc caccaccgat gtcgacgttc gtgccccagg cgaagtgctc tgaggtccat
     5701 gcagctgcgc ctgaccctcg ccctgcaagg tgcgactcaa ggctgggacc cgcgacggtc
     5761 gagacgaagt cgcctgccag tggagccacg atgtcgagaa ccctctcggc gttctggctc
     5821 ttggcggact cgccggtgat gatcactgcg ccggtgtcga tctgatccgg cgtgaacccg
     5881 gcttccttgt agtccgccca caggatcttc tcgagctcgt cgacgtcaac gagtccgtcg
     5941 tggtcgagtg gcgtgaagtg caccgggctc tgatacagga cgtccttctt tgccacgtct
     6001 gcccttggct tccgcgctgg accatacctt gcctgcggca tcttgctcaa tgtcgcggtc
     6061 tcggcgacca gcagtttcga gatgacgatc tgtgtggtcg tggttccgat atcgatgccc
     6121 acactgatga gttcgaccga tgccattact ctgcctccgc tacttttccg acttccggct
     6181 gttctttcca ggattgttct tcttgtcgac gtacaggtgt tccttgagtg aagtcacgag
     6241 ggttttcttt gctccctgga ccttcttcag ctcttcgacc gagctgaatg gtccctgctg
     6301 tgcgcgaaat tcgacgatgc gctcagctaa cgccggtcct acacctggca aggcgtccag
     6361 ctgctcggcg gtcgccgtgt tcaggtccag gcgtcgtgct actgtagcgt gcaccgaccc
     6421 cggaggttcg ccagacaatc caacaggtat gactggcatc acgacggagg gttcctggat
     6481 actggtcgca ggggcgatga ctctcgcggc gtcagttgcg gtcacgggct gagaggaaac
     6541 atgcgagatc ggctgaacca ccggctcgac tgctcctgag actggggaag gttcagcgct
     6601 caaagtcccc ggagtgtgcg ctgaagcctc actgggcgca ggggacgctc cttcgccgac
     6661 gggaaggccg agattcttgc tcatattgtc gataatcagg tcaatgtcct tgtcggggcg
     6721 cgcaatcacg tgaatcgaca ccatctcgcc gacgcgccct gccgccgtcg ctcctgcatc
     6781 cgtggcagcc
//
LOCUS       DATMZD010000248         3385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_127617,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000248 DATMZD010000000
VERSION     DATMZD010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3385
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127617"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..133)
                     /locus_tag="VN478_05150"
     CDS             complement(<1..133)
                     /locus_tag="VN478_05150"
                     /inference="COORDINATES: protein motif:HMM:NF014077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HWQ22093.1"
                     /translation="MSATRKARRARILLTNDDGIFANGINAVAGVLAEHYELTIVAPE
                     "
     gene            complement(143..1510)
                     /locus_tag="VN478_05155"
     CDS             complement(143..1510)
                     /locus_tag="VN478_05155"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWQ22094.1"
                     /translation="MSVKLTFYGAVNEIGGSKTRLEYGGTNLFLDFGKDFVGSTDHFS
                     DRLKPAAYLQVRDYLRFGNLPPMAALYPPAQSQFLQQEFRAALDSGTVEGILLSHGHI
                     DHVGFLPLVDVSVPLYMSAETLEVLRFFDETGQFDLRMDQRHVTVFEHDKVMRIGDCE
                     VLPVRSDHDILGILGFIVRCGDTSIIYTGDYRLHGKHPERVRTFFELMRQEKQRAKTV
                     LLTEGTRLGFGSVDQLTESEIQELATRAVGMASGLVMTNYYILDTDRLKVFLRVAEKT
                     GRTMVLQPQHMLMARKFARFDSELERLIKNAEVYLARRGSGRYLAPDYQLFEQEHLVT
                     ALRAADIAAEPARYMLQLDFPDLGELVEIDPAPHTVFIQSGGEPLGQYDPNYGILMKW
                     VKDFRMDFYRIATPGHASELDIRDLVMRADPSVLVPMHSKAPEALSGYVKDTVALKKG
                     VTYEF"
     gene            1817..2914
                     /locus_tag="VN478_05160"
     CDS             1817..2914
                     /locus_tag="VN478_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWQ22095.1"
                     /translation="MARVVLENVTKRFGKVIAVNNVSLEAEDKEFLVLLGPSGCGKTT
                     TLRMIAGLELADEGNVIIGDDIVNDVPPAARDIAMVFQNYALYPHMTVRDNISFGLRL
                     HNIPAGEIKDRVADAAQMLGLQDYLKRVPKELSGGQRQRVALARAIVRKPKVFLMDEP
                     LSNLDAKLRVQTREELIKLHQTLGVTTIYVTHDQVEAMTLGSRVAVMENGFLRQVGKP
                     RQVYDNPTNKFVAGFVGTPPSNFVPVNLVLTPTPALEVEGQRVELSSTQQQAARTVPA
                     GSYAWGVRPEDLLVDGQSNMPEVAAAFDAKISFVEMMGSETFIHTTLAGAPVVIRASS
                     AFEYGQGDVVKLVLNVARTHLFHADSGEAVF"
     gene            3034..>3385
                     /locus_tag="VN478_05165"
     CDS             3034..>3385
                     /locus_tag="VN478_05165"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HWQ22096.1"
                     /translation="MTDEIRFTGFGDMIAASIGDWHVPGCAVTAVRDGQTVLCETRGL
                     RDVEQRLPFLTGTLFEIASLTKAFTAAALGILADRHLIDFDKPVRTYLPGFTLYDTAA
                     TDTVTITDLLSHSTG"
BASE COUNT          681 a         1030 c          975 g          699 t
ORIGIN      
        1 cttccggggc aacgatcgtg agttcgtagt gttccgccag tacacctgca acagcgttga
       61 taccgttggc gaagatgcca tcatcgttgg taagcaggat tcgtgcacgt cgggccttcc
      121 tggttgcgct catgtcagct cctcagaact catatgtcac gcctttcttc aatgcgaccg
      181 tgtccttgac atacccgctc agagcctcgg gagccttgga gtgcatgggt accaggacgc
      241 tggggtccgc ccgcatgacc aggtctctga tgtcgagttc gctcgcatgc cccggtgtgg
      301 caatgcggta gaagtccatc ctgaagtcct tgacccattt catgaggata ccatagtttg
      361 ggtcgtattg ccccagaggt tcgccgccgg attgaatgaa gacggtatgc ggagccgggt
      421 cgatctcgac gagttcgcca agatcgggga agtccagctg cagcatgtac ctggctggtt
      481 ctgcagcgat gtctgcggca cggagagcag tcaccaggtg ctcctgttcg aacagctgat
      541 agtcgggcgc caggtacctg cccgagccac gcctggcaag gtacacttcg gcattcttga
      601 tgagccgttc gagctcgctg tcgaaacggg cgaatttccg ggccatcagc atgtgctggg
      661 gctgaaggac cattgtccgc cccgtcttct cggcgacacg caggaagacc ttcagacgat
      721 ccgtgtccag gatgtagtag ttggtcatca cgagaccgga agccatgccg accgccctcg
      781 tggcaagttc ctggatctca ctctcagtca actggtcgac actgccgaat cccagcctgg
      841 tcccctccgt gagaagcaca gtcttggcac gctgcttctc ctggcgcatc agctcgaaga
      901 atgtacgtac tcgctctgga tgcttgccat gcagccgata gtcccccgtg tagatgatcg
      961 acgtgtcgcc gcaccggacg atgaacccca ggatgcccag tatatcgtgg tcactgcgga
     1021 caggcaggac ttcgcagtct ccgatgcgca tgaccttgtc gtgctcgaat accgttacgt
     1081 ggcgctggtc catgcggagg tcaaactgcc ctgtctcatc gaagaatcgc aggacctcaa
     1141 gcgtctcggc actcatgtac aacggtacgc tgacgtcaac aaggggcagg aacccgacat
     1201 ggtcgatgtg tccatggctc aacaggatcc cctctactgt ccccgagtcg agggcggcac
     1261 ggaattcctg ctgaaggaac tgagactgag ccggcggata caatgcagcc atgggcggca
     1321 gattgccaaa ccggaggtag tcgcggacct gcaggtatgc agccggtttc agccgatcac
     1381 tgaagtggtc cgtgcttcca acgaagtcct tgccaaaatc gagaaacagg ttcgtgccgc
     1441 cgtactccag gcgggtcttg ctcccgccta tctcgttcac tgcaccatag aaggtcaatt
     1501 tgactgacat gcttcctcca tgcaatccag ttccgagcac aaccatcgtg ccacgtgcaa
     1561 agcaaagtat tataccttct cggccgggtc cggcaagtcg tcgagagtcc tggaacctcg
     1621 cgccgtgcat ctctgacagt ctccgggttc cctggacctg ttgcagaacc cttccgttgc
     1681 aaacggccga ccgctgacta aactgacaga gataccagag tacgggatcg aggagcgtgc
     1741 tgctcctgag actggtgaag gctgggcatg aacgcggtcc cctggcgccc ggacaagtcc
     1801 gcaagtggag gctgcaatgg ctagagtcgt tctggagaac gttacgaagc ggtttggcaa
     1861 ggtcatcgct gtcaacaacg tgtcgctcga agccgaggat aaggagttcc ttgtcctgtt
     1921 gggaccctcc ggctgtggga aaacgacgac cctccgcatg atcgccggcc ttgaactggc
     1981 tgacgaaggg aacgtgatca tcggcgatga catcgtgaat gacgtcccgc ccgctgcccg
     2041 tgacatcgcc atggtgtttc agaactacgc cctgtaccct cacatgaccg tacgcgacaa
     2101 catctccttt ggcctgaggt tgcacaatat cccggctggg gagatcaagg accgcgtcgc
     2161 ggacgctgca cagatgctgg gactgcagga ttacctgaaa cgggttccca aggagctttc
     2221 cggtggccag cgccagcgcg ttgctcttgc ccgtgccatc gttcgcaagc ccaaggtctt
     2281 ccttatggac gaaccactgt ccaacctcga cgccaagttg cgcgtgcaga ctcgcgagga
     2341 actgatcaag ctgcaccaga ccctgggcgt tacgaccatc tacgtcacgc acgaccaggt
     2401 cgaggccatg acgctgggct cacgtgtcgc cgtcatggag aacggcttcc tgcgccaggt
     2461 cggcaagcca cgccaggtgt acgacaaccc aacaaacaag ttcgtcgctg gatttgtcgg
     2521 cactcccccc tccaatttcg tccccgtcaa cctcgtcctg accccgacac cggcactgga
     2581 ggtcgaggga cagcgcgtag aactgtcctc cactcagcag caggccgcca ggacagtgcc
     2641 cgccggcagc tatgcatggg gcgtccggcc cgaggacctg ctcgtcgacg gtcaatccaa
     2701 catgcctgag gtcgcggcag cgtttgacgc caagatctcg tttgtcgaga tgatgggcag
     2761 cgagaccttc attcatacga ctctggccgg cgctcccgtc gtcatacggg caagcagcgc
     2821 cttcgagtat ggtcagggtg acgtggtcaa gctcgtgctg aacgtggctc gcacgcacct
     2881 cttccacgcg gactcaggag aagccgtctt ctagtgctgt cgtagcccgg acggtctcat
     2941 cctcgccagc atgctgtttc ttgaaccccg ccgtgactgt ggcggggttc ttcctatact
     3001 gggaccagac acaaccatac gggggaaact gcaatgactg acgaaatccg cttcaccggc
     3061 tttggtgaca tgatagctgc ttccatcggg gactggcacg ttcctggatg tgcagtaacg
     3121 gctgtacgag acggtcagac ggtgctgtgt gaaaccagag gcctgcgtga cgtcgagcag
     3181 cgtctgccct tcctgacagg gacactgttt gagatcgcat cgctgaccaa ggcgtttacg
     3241 gctgccgctc ttggcatcct ggcggatcgg catctgatcg acttcgacaa acctgtccgc
     3301 acgtacttgc cgggcttcac gctgtatgat accgccgcga cagataccgt caccatcact
     3361 gaccttctga gccacagcac cggat
//
LOCUS       DATMZD010000249         2083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_57769,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000249 DATMZD010000000
VERSION     DATMZD010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57769"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..282)
                     /locus_tag="VN478_05170"
     CDS             complement(4..282)
                     /locus_tag="VN478_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HWQ22097.1"
                     /translation="MNKGELVARIADMTKMTKKESEVMVNAFMDAVKEAMKKNDKVAL
                     VGFGTFGVKERKARKGVNPKTLKAITIPKKKAPFFKPGKALKDAIAKK"
     gene            798..1259
                     /locus_tag="VN478_05175"
     CDS             798..1259
                     /locus_tag="VN478_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HWQ22098.1"
                     /translation="MAIEEKLAELGLTLPEAGVPVGSYVPVKIAGNLAFVSGMLPTEA
                     GRVKQTGRVGEDITTDEAYSLARVCAVNGLAALKGAIGTLDRVKGIAMVQGFVQGAPG
                     YAEVPKVINGASDLLVALFGDAGRHARFAVGVASLPMNAPVEVAFVAELMA"
     gene            1349..1822
                     /locus_tag="VN478_05180"
     CDS             1349..1822
                     /locus_tag="VN478_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HWQ22099.1"
                     /translation="MTSEIRVAMKVNGTRYHITTQANRTLAEVLREDLGLLGTKIGCN
                     KGECGACTVIMNGRPVTSCLVLAVQAAGATVTTIEGLADGAGPSLLERSFVEEGAVQC
                     GFCTPGMVMSAEALLAVNPHPSEVEIETALEGNLCRCTGYRKIVAAVQKASQSRR"
     gene            1819..>2083
                     /locus_tag="VN478_05185"
     CDS             1819..>2083
                     /locus_tag="VN478_05185"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HWQ22100.1"
                     /translation="MTAAVVLAAGHSDRMGSNKLALPMGSGTVVGRVVEIALAACDSV
                     IVVIALHDQATRTAVERTAAALGTQERVQVTEGVPYDPGMFISI"
BASE COUNT          409 a          557 c          671 g          446 t
ORIGIN      
        1 aaactacttc ttggcgatgg cgtccttcag agccttgccg ggcttgaaga agggggcctt
       61 ctttttcggg atggtgatgg ccttgagggt cttggggttg acgcccttgc gagccttgcg
      121 ctccttgacg ccgaacgtgc cgaaaccgac gagtgcgacc ttgtcgttct tcttcatggc
      181 ttccttgacg gcgtccatga acgcatttac catgacctcg gattccttct tggtcatctt
      241 cgtcatgtca gcgattctgg ctacaagttc tcccttgttc atgcaatctc ctttcgtgtt
      301 ttttatcgcg cttttccccg cgactgggaa ggtacgcacg tagtatagca cattttgaca
      361 aaatcgggta gcgttatcgt cgcttttcat gggagatgac agcaagacga gcccacgata
      421 gggcatcacc gcgtggtgat atgcagtcct ggactgcagt ctagcggttg gacaggaaac
      481 ttgcacctca tgcaacataa gctgggtcct ggatcagatt tgtcaagact gaccaaggaa
      541 agctgggaga gccacaggtc agacatcgtt aaactgaccg tatcgggccg attcttgctc
      601 cttcaaggtt gtttgtgcgt gatgacagat tccgaaccgg aacatagcga ccacccatgg
      661 gagtgtcggc cacgcaaggt ggctggacag cacttgccgg cgcgctgacg gttgccgccg
      721 cagtgtggac gtggcaagag tccgcttgtg tgtatactat gcgtgagaac tcgtccacat
      781 cataccggga ggtacatatg gcgatagaag agaagctggc ggagcttggg ctgacattgc
      841 cggaagccgg agtgcccgtt ggcagttacg tgccggtcaa gattgccggc aacctcgcct
      901 ttgtttcggg aatgctgccc acagaggccg gtcgtgtgaa acagaccggt cgagtgggag
      961 aggacatcac gacagatgag gcatacagtc tcgcacgcgt ctgtgccgtg aatggactcg
     1021 cagccctgaa gggagctatc ggcacactgg accgcgtcaa gggcatcgcg atggtgcagg
     1081 gattcgtaca gggagctcca ggctatgcgg aagtcccgaa ggttatcaac ggggcttctg
     1141 acctgcttgt cgcgcttttc ggcgatgccg ggcgccatgc gcgtttcgcg gttggagtcg
     1201 cgtcgctccc catgaacgct cccgttgagg tcgctttcgt tgccgagctg atggcctgag
     1261 agggaccccg tgccgttcgc ggtcctgggt cttcgcgcag agagcgccgg gcgccatcgc
     1321 tatggaacct gtacaggggg tagacagaat gaccagtgag attcgagtcg ccatgaaggt
     1381 caacggaacc aggtaccaca ttacgaccca agccaacagg acgctagccg aggttctgcg
     1441 ggaggacctg ggactcctcg ggaccaagat cgggtgcaac aagggcgagt gcggggcatg
     1501 cacggtcatc atgaacggcc ggccggtcac cagttgtctg gtcctggcgg tgcaggcggc
     1561 gggagcgacc gtcacgacga tcgagggtct ggctgatggg gcgggccctt cgctgctcga
     1621 acggtcattc gtcgaggagg gagctgtcca gtgcggcttc tgcactccgg gaatggtcat
     1681 gtccgccgag gcgctgcttg cagtgaaccc gcatccatcg gaggtagaga tagagactgc
     1741 cctggagggg aatctttgcc gttgcacggg ctacaggaag atcgtcgccg ccgttcagaa
     1801 ggcaagtcag agtcgcagat gactgcagca gtggttctgg cagctggaca ctccgacagg
     1861 atgggctcaa acaagctggc cctgccaatg ggctctggaa cggtcgtcgg ccgtgtcgtc
     1921 gaaattgcgc tggccgcctg tgacagtgtg atcgtcgtca ttgcattgca tgatcaggca
     1981 acgaggactg cagtcgagcg gacagccgcg gcactgggta ctcaagaacg ggtccaggtg
     2041 accgaggggg tgccctacga tcccggcatg ttcatctcga ttc
//
LOCUS       DATMZD010000250         6145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_147671,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000250 DATMZD010000000
VERSION     DATMZD010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6145
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147671"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..631
                     /locus_tag="VN478_05190"
     CDS             137..631
                     /locus_tag="VN478_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22101.1"
                     /translation="MTPFSDIFDLNFFIYLGFVVLAVAGLVMAIASRRSQAGRVSLFL
                     LLTTALVTSYYTLQWLFNSFAVYFINNYMVSHPDFQVPDWTMIVLNYLVLALNVGFVA
                     TLALTAWFSVRKSHAEKSALAQSLEETGSEEVTSEVSVDAGQFATPGDDVAPVDPPVA
                     GPEV"
     gene            635..1186
                     /locus_tag="VN478_05195"
     CDS             635..1186
                     /locus_tag="VN478_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HWQ22102.1"
                     /translation="MDREDLYQVIVERAPSRSLVNHMLAVEAIMRGLAMHFHEDAEKW
                     GIAGLVHDIDYAETKDKPNLHAIVGSEQLHLMGVSEEVCYAVRVHNPLHGLPRKSLMD
                     KALYAADPLSGLIVACALIQPDKRLASIDTDFVMARFKEKRFAAGANRDQILACQEMG
                     LDLYRFVVIGLTSMKAVAGEIGL"
     gene            1340..2548
                     /locus_tag="VN478_05200"
     CDS             1340..2548
                     /locus_tag="VN478_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HWQ22103.1"
                     /translation="MSLNAEELFSERALGFRPSEIRELLKLVDSPEIISLAGGMPDDR
                     FFPIDRVIEASTFALREYGKKALQYGSTEGIKKLRVLLMDRMEKEGVSGVDLENVIVS
                     TASQQGLSLVAQVFVNPGDTVIVEEPSYLGAIQACASMQAKFCTVPLDKDGMQMDILE
                     DRLKELQKANIRPKFVYTVPNFHNPAGVTMTLERRKKLIELAQTYNLLIVEDDPYGEI
                     RFEGEPIPSLLALGGKERVVALRTFSKISFPGLRLGWIVAREDIMNKIIVGKQAADLC
                     SPAMTQYISYEFISRGWLDDYVEVVRREYPKKKNAMISALGQYFPADASWTDPQGGLF
                     VWAKVSDSIDTAAMFREAIAAKVAYVVGIAFYPHRNDNCHMRLNFSAVDPEHITEGIH
                     RLGDLLKSKM"
     gene            complement(2634..3407)
                     /locus_tag="VN478_05205"
     CDS             complement(2634..3407)
                     /locus_tag="VN478_05205"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HWQ22104.1"
                     /translation="MEDAIAEPLSYSRVSTYAECPRKFELKYVQKIPEPAHWYFSYGK
                     SIHAVLERLLSCHLREDGLLYGDDAALFPTSLSQLLDENWLTEGYADPVDEQRKRRQA
                     LRVLTAFFPVFQSTWQQTIYVEHLINTKIDGIPFVGIVDRIDRIDDNAIRIVDYKSAK
                     PREASALTSHRFQVALYASALSQLNEFRGKRFILQTYYLRENVKAEMDAGAENCIAKS
                     REVISATADRIAKGDFRGRRSNRCFSCDYRDVCPVAHHA"
     gene            complement(3380..3979)
                     /gene="coaE"
                     /locus_tag="VN478_05210"
     CDS             complement(3380..3979)
                     /gene="coaE"
                     /locus_tag="VN478_05210"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HWQ22105.1"
                     /translation="MNIDVGLTGNIASGKSMISGMLRDHGAAVLDADVVGKIVLADNV
                     EGALEQVRSAFGDGVFDGRQLDRHALGNLVFGHPELLIRLNSIMMPIMTRLVAKEIAA
                     LHQVSSVVVLDAAILVEAGWQNLVDEIWVVKASKEEQLERLMTRDRLSHDEAVSRIEA
                     QMPLAVKLSYADVIIDNTMDVERTRRQVEILWKTRLQNR"
     gene            complement(3976..>6145)
                     /gene="polA"
                     /locus_tag="VN478_05215"
     CDS             complement(3976..>6145)
                     /gene="polA"
                     /locus_tag="VN478_05215"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HWQ22106.1"
                     /translation="LTDENTSVRITVKGVKEVREYTPAAILDEFGLRSSQLVDVKALE
                     GDSSDNIPGVRGIGEKTALRLIREFGSVQALLENGGPERYRELLAENRDLIMRNRSLV
                     TIQMDLDEPTSLPDLIRNTPDIDQSAQMFQALSFVTLSRRASKVLEAPSLAVSRVPVP
                     HDDLFSSGVRPAAIAPHPLQPANTVLDGRTALAILPDAQGNGFSIARRTKSSTSSTHV
                     DVMFGSPSEILHSPGPSMTYVYDYKRLCHVFAPFGLDVPAPVRDIMLAQYLLDPDQKA
                     WGLDRIGDAWGLSGDTSTADGMAELVYRVSYPIDTALEESHLTSTLDEMEIPFARVLA
                     NMEMTGIRTDTGYLRTFGAKLGLRIAELERKAYELVGTHDYSLQSPKQLGTLLFDVLG
                     LVPTKRTKTGLSTDVESLEAIQNEHPAIPLILELRQLTKIKGTYTDSLARYVAQDGKI
                     HPTFLQTGTSTGRISCVDPNLQNIPARTEVGREIRQAFIPSHDGWALVSADYSQIDLR
                     MLAHLSGDANLCNAFIQGEDIHLHTASQVFDIPESAVTPEMRKRAKTINFGIIYGISP
                     FGLSRQLGIGAEEARQYIERYLQRFPGIKDYRDRVVEAAQRDGYVKTILGHLRRVPHI
                     NSHNNVERQEAIRQGFNAVVQGSSADVIKMAMISLDKELQPLRARMVLQVHDEIVVDT
                     PPDELERVRQLMQASMESAIHLSIPLVTHLTAGPNLRDLQ"
BASE COUNT         1237 a         1687 c         1850 g         1371 t
ORIGIN      
        1 ccaggaaacc ggctgcttcc acggcgatag cagcgaaggc aaaggcagcg gtggccggta
       61 agggtacttc gacgaagaag ggtggttcaa cgaagaaggc gattccaaag acatccaaag
      121 acagaggtgg tgcgtcatga ctccattcag cgacattttc gatctcaact tcttcatcta
      181 tcttggattt gtggtcctgg ctgtggcggg gcttgtgatg gcaatcgcct ccaggcgctc
      241 tcaggcagga agggtgagcc tgttcctcct gctgacgacg gcgctggtga cttcgtacta
      301 cacgctgcag tggctgttca attccttcgc cgtctacttc atcaacaact acatggtttc
      361 ccatcctgat ttccaggtac ccgactggac catgatcgtg ctcaactacc ttgttcttgc
      421 tctgaacgtc ggattcgtag cgacgttggc cttgaccgca tggttttcag tccgaaagag
      481 tcatgccgag aagtccgccc ttgctcagag tctcgaggag actggcagcg aagaggtgac
      541 ttccgaggtc agtgtggatg ccgggcagtt cgccacgccg ggcgatgatg tcgctccggt
      601 cgacccccct gtcgccggtc ccgaagtgta gtccgtggat cgcgaggacc tctaccaggt
      661 catcgttgag cgagcaccct cgcggagcct ggtgaaccac atgcttgctg tcgaggctat
      721 catgcgcggg ctcgccatgc acttccatga ggacgcggag aagtggggca ttgcagggct
      781 ggtccacgac atcgactatg ccgagaccaa ggacaagccg aacctgcacg caatcgtggg
      841 atccgagcag ttgcatttga tgggagtgtc ggaggaggtc tgctatgcgg tccgtgtcca
      901 taacccgttg cacggactgc cccgcaagag cttgatggac aaagcactct atgctgcaga
      961 cccactgtct ggactcattg tcgcgtgtgc cctcattcag ccggacaaga gacttgcatc
     1021 tatcgacacc gacttcgtca tggctcgctt caaggagaag cgctttgctg ctggtgcgaa
     1081 ccgcgaccag attcttgcct gtcaggagat gggactggat ctgtaccgat tcgttgtgat
     1141 cggtctgacg tcgatgaagg ccgtcgccgg ggagatcggt ctgtagacag ccctcggcac
     1201 tttgcacggc cacagaagcc cgcccgagag gcgggcttct cgttatgccc aatagggata
     1261 tacttcattg gtaagtagtc tcgtatgctc tgcacgatgg acacttgccg agatctcact
     1321 gtgatgagga ggcttatcga tgtcgttgaa tgcagaagag ttgttctcag aaagagcact
     1381 gggtttcagg ccgtccgaga ttcgggagct cctcaagctc gtcgacagtc cggagatcat
     1441 ctcgttggcc ggtggcatgc cggacgaccg atttttcccg atcgaccgag tgatcgaggc
     1501 cagcacgttt gcactcagag agtacggcaa gaaggctctc caatacggat ctaccgaggg
     1561 catcaagaag ttgcgtgtcc tgctcatgga tcgtatggag aaagaaggtg tgagcggcgt
     1621 cgacctggag aacgttatcg tctcgactgc ctcgcagcaa ggtcttagtc tggttgcgca
     1681 ggtattcgtg aatccgggcg acacggtcat cgtcgaagaa ccttcatatc ttggggcaat
     1741 ccaggcctgt gcgagcatgc aggccaagtt ctgcactgtg ccgctggata aggatggcat
     1801 gcagatggac attctcgagg acaggctcaa ggagttgcag aaggcgaaca tccgtccgaa
     1861 gttcgtctac acagtgccca acttccacaa cccggcgggc gtcacgatga cgcttgagcg
     1921 ccgcaagaag ctgatcgagc ttgcacagac gtacaatctg ctcatcgtcg aagacgaccc
     1981 gtacggggag atccggttcg aaggtgagcc gattccgtca ttgctggcgc ttggcggtaa
     2041 ggaacgcgtc gtggcgctgc gcacgttttc gaagatcagt ttccccgggc ttcgcctcgg
     2101 atggattgtt gcacgcgaag acatcatgaa caagattatc gttggaaagc aggcggctga
     2161 cctgtgttca ccagccatga cccagtacat atcatacgag ttcatcagcc ggggttggct
     2221 ggatgactac gtcgaggtcg ttcggcgcga gtatcccaag aagaagaatg cgatgatctc
     2281 cgccctggga cagtactttc cggcagatgc cagctggact gacccgcagg gtggcctgtt
     2341 cgtctgggcg aaggtgtccg atagcatcga cactgccgcg atgttccgcg aggccatcgc
     2401 agccaaggtc gcatatgtcg tgggaattgc cttctacccg caccggaatg acaactgcca
     2461 catgcggctg aacttctcgg ctgtagatcc agaacacatc accgagggga tccatcgtct
     2521 gggcgacctg ctgaagtcga agatgtagcg gcgagctagg acagacgttt gggaaacgac
     2581 ccctgccaca tcacgcgtgg caggggtcgt caggtctccg agcggtgcag gaactaggca
     2641 tgatgagcca cagggcaaac gtctcgatag tcacacgaga agcacctgtt gcttcgcctt
     2701 ccacgaaagt cgcctttggc aatcctgtcc gccgttgcac tgataacctc gcgggacttg
     2761 gcaatacagt tctccgcccc tgcatccatc tcagctttga cgttctcacg gagatagtac
     2821 gtctggagga tgaatcgttt gccccgaaac tcattcagct ggcttagcgc tgaggcatag
     2881 agtgccacct ggaaccgatg agacgtcagt gctgacgctt ctctcggctt tgcggacttg
     2941 tagtcgacga tgcggatcgc gttgtcgtcg atgcgatcga ttcggtccac aatacccaca
     3001 aacgggattc cgtcgatctt ggtattgatg aggtgttcta catatatggt ctgttgccat
     3061 gtcgactgga atacggggaa gaaagccgtg agcacgcgaa gggcttggcg ccgcttcctt
     3121 tgctcgtcga cgggatcggc gtacccctcc gtcagccagt tctcatcgag aagctgagac
     3181 agagaagtgg gaaagagggc tgcatcgtct ccgtacagca gtccgtcttc tcgcaagtgg
     3241 catgacagta gtctctcgag gaccgcgtgg atgctcttgc catagctgaa gtaccagtgg
     3301 gctggctccg ggatcttctg aacatacttg agctcgaatt tccgtggaca ctcggcgtat
     3361 gtcgatactc tcgagtagct cagcggttct gcaatcgcgt cttccaaagt atctccacct
     3421 gtcttcgcgt ccgctcgaca tccatcgtat tgtctatgat gacgtcggcg tacgagagtt
     3481 tcacagccag cggcatctgt gcctcaatgc ggctgacagc ttcatcgtga gagagacgat
     3541 cacgcgtcat gagccgttcc agctgttctt ccttcgaggc cttcacgacc cagatctcgt
     3601 cgaccagatt ctgccagcca gcctcgacga gaatagctgc gtcgaggacc acgacgctcg
     3661 agacttggtg aagtgcagcg atctctttgg cgacgagcct cgtcatgatg ggcatcatga
     3721 tactattgag tcttatcagg agttcggggt gcccgaagac caggttgccg agggcgtgac
     3781 ggtcgagctg ccgtccatca aagacgccgt cgccgaatgc agatcgcacc tgctcaagag
     3841 cgccctcaac attgtcggca agaacgatct tgcccacgac gtctgcgtcc agtactgccg
     3901 ccccatgatc ccgcagcatg cccgagatca tgctcttgcc agatgcgata ttgccggtca
     3961 ggccgacgtc gatgttcatt ggaggtctcg gagattgggg ccagctgtga ggtgcgtgac
     4021 gaggggaatc gagagatgta tcgctgactc catggatgcc tgcatcaact gacgaacacg
     4081 ttcgagttcg tcgggcggtg tgtcgacgac gatctcgtcg tgcacctgga ggaccatccg
     4141 ggccctgaga ggctggagtt ctttgtccag cgatatcatc gccatcttga tgacatctgc
     4201 cgaactccct tgtacgactg cgttgaagcc ttgccggatc gcctcctgcc gctctacgtt
     4261 gttgtggctg ttgatgtgcg gaactctgcg caggtgtccg aggatcgttt tcacgtagcc
     4321 gtcgcgctgt gctgcctcga ccacgcggtc acggtaatcc ttgatgccag ggaatcgctg
     4381 cagatagcgt tcgatgtact gccgagcctc ttcggcgcca atgccgagct gtcgtgacag
     4441 gccgaatggc gatatgccgt agatgatgcc gaagttgatc gtcttggcac gtttgcgcat
     4501 ctccggagtc acggcggact ccgggatgtc gaagacctgt gaagccgtgt gcaggtgaat
     4561 gtcctcgccc tggatgaatg cattgcagag gttggcgtct ccagaaaggt gggcgagcat
     4621 tctcagatca atctgggaat agtctgctga gaccagtgcc cagccatcat ggctgggtat
     4681 gaaagcctgt ctgatctcgc gccccacctc ggttcttgcc gggatgttct gcaggttggg
     4741 atcgacacag ctgatgcgtc ccgtcgatgt gccggtctgg aggaaggtag ggtggatctt
     4801 cccgtcttgt gcgacgtatc gcgccaacga gtctgtatat gtccccttga tcttggtcag
     4861 ctgacgcagc tccaggatga gtggaattgc cggatgttcg ttctgtatcg cctccagcga
     4921 ctcgacgtcg gtgctcagtc cggtcttcgt cctcttggtc ggtacaagtc ccagcacgtc
     4981 aaagaggagg gtgcccaact gcttcggaga ctggaggctg tagtcgtgcg tgcccacgag
     5041 ctcatatgcc ttgcgctcca actcggcgat acgaaggccc agtttcgctc caaacgtccg
     5101 caggtaaccg gtgtctgtgc gtatgccggt catctccata ttcgcgagca ctctggcaaa
     5161 agggatttcc atctcgtcca gagtgcttgt gagatgtgac tcctcaaggg ctgtgtcgat
     5221 gggataggaa acccgataga cgagttctgc cattccgtcg gcggtggatg tatctccaga
     5281 gagtccccag gcgtcgccga tgcggtcaag gccccaagcc ttctggtcag ggtcaagcag
     5341 gtactgtgcc agcatgatgt ctcgaacagg ggcaggaaca tcaagaccaa acggcgcgaa
     5401 gacgtgacac agtctcttgt agtcatagac ataggtcatc gatgggccgg gagaatggag
     5461 gatctcggaa ggtgagccga acatgacatc gacatgagtc gaggaagtac tgctcttcgt
     5521 tcggcgggct atgctgaacc cgttgccctg ggcgtcgggc aggatggcca gtgcggtccg
     5581 gccatcaagg actgtgttcg caggctgcag gggatgggga gcgatggctg cggggcgaac
     5641 gccggagctg aagaggtcat catgagggac aggcacccgc gacacggcca gtgatggtgc
     5701 ctcgaggacc ttcgacgcac gccggctcag tgtcacgaac gagagtgcct ggaacatctg
     5761 ggcgctctgg tcgatgtctg gggtgttgcg gatcagatcg ggcaggctgg tcggttcgtc
     5821 cagatccatc tggatcgtga ccagtgaacg gttccgcatg atgaggtctc ggttctcggc
     5881 gagcagttcc ctgtagcgtt ccgggccgcc attctcgagc aatgcctgca ctgagccgaa
     5941 ttcccgaatc agcctgagtg cggtcttctc gccgatgccg cgtactcctg gtatgttgtc
     6001 cgacgagtct ccctcaagcg ccttcacatc gactagttga gaggatctga gtccgaactc
     6061 gtcaaggatt gccgcgggag tgtactccct gacctctttc acgcccttga ccgtgatgcg
     6121 cacggacgta ttctcatcag tcagc
//
LOCUS       DATMZD010000251         2493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_67311,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000251 DATMZD010000000
VERSION     DATMZD010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67311"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1195
                     /locus_tag="VN478_05220"
     CDS             <1..1195
                     /locus_tag="VN478_05220"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HWQ22107.1"
                     /translation="FPVKNYGIIKVIDEKKMYADCKAVFDDLKLDIDPATKVSNLSSS
                     KIQLMEIAQAVSYHASVIIMDEPTSSLTENEVQHLFTIIRRLKEQGIAIVYISHRMEE
                     IFEISDEVTIMRDGKCVGTYPTRELTSEFVIKQMVGRTLTNLFPPRDNTPDGVILSVE
                     GLTSAAEHSFKDVTFDVKKGEIFGIGGLVGAQRSELVEALFGLRHVASGTVSINGTKA
                     VITNPIQAKQYRMALLTEERRDTGIFPVLSVLDNTLIANLGRYVHRAGLLDEKAGSRD
                     AEKIIATLRVKTPALATLIENLSGGNQQKVIVARWLLTQPDILILDEPTRGIDVGAKY
                     EIYSIIADLAKSGKTIIMISSEMPELIGMSDRIMVMCEGRVTGILDGKEATQERIMTL
                     ATQLM"
     gene            1233..2252
                     /gene="mglC"
                     /locus_tag="VN478_05225"
     CDS             1233..2252
                     /gene="mglC"
                     /locus_tag="VN478_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018884737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HWQ22108.1"
                     /translation="MSKRVTARSVREFAVRNAIILVLAVLVLVIGIIKNGFLTPGNLV
                     NIMRISSVRLIIALACAGALITGGTDLSAGRTVGLAAVIVASLVQRPDYASKIFPNLP
                     YMWVIFPILIAVAAGLLVGIINGVVIAYLKVPPFIATLGMMVVVWGAASLYVDRPPLG
                     AQPIGGLRLDFTNLGTGSIGFGRFAIPTIILIALIVFAFYWVMLNKTQLGKNMYAIGG
                     NVNAAIVSGVNVKRNLVAVYAIAGTLYGLAGVLLAARSGGATNNYGQSYELDAIAAAV
                     IGGVSTSGGRGTVVGVLTGVLIFEVLNNGLVVLGVSAYWQQVVKGIIIVAAVALDIRK
                     YSKRQ"
BASE COUNT          524 a          728 c          728 g          513 t
ORIGIN      
        1 ctttccggtg aagaactacg gcatcatcaa agtcatagat gagaagaaga tgtacgctga
       61 ctgcaaggcg gtcttcgacg acctgaagct ggacatcgat cctgcgacga aggtgtccaa
      121 cctgtcatct tcgaagatcc agctcatgga gattgcacag gcagtctcct atcacgcaag
      181 cgtcatcatc atggatgagc cgacgtcatc tctcaccgag aatgaggtgc agcatctctt
      241 tacgatcatc aggagactga aggagcaggg tatcgcgatc gtctatatct cccacaggat
      301 ggaggagatc ttcgagatct ctgacgaagt cacgatcatg cgcgacggca agtgtgtcgg
      361 cacctatccc acacgcgaac tcacgtccga gtttgtcatc aagcagatgg tgggtcgcac
      421 cttgacgaac ctgttccccc cgagagacaa cacgccggac ggcgtcattc tcagtgtaga
      481 aggccttacg tcggcggctg agcactcctt caaagacgta acgtttgacg tgaagaaggg
      541 tgagatcttc ggtatcggcg gcctcgtcgg agcacagcgg agcgaactgg tcgaagcact
      601 gtttggactg cgccatgtcg cgagcggcac agtgagcatc aatggcacaa aagccgtcat
      661 caccaacccc atccaggcca agcagtacag gatggcactc ctgaccgaag agcgacggga
      721 caccggcatc ttcccggtgc tgtccgtgct ggacaatacg ctcattgcaa accttggccg
      781 gtacgtacac agggccggcc ttcttgacga gaaggcaggc agccgtgatg ccgagaagat
      841 catcgccacg ctcagggtca aaacccctgc attggcgacc ctgatcgaga acctttccgg
      901 cggaaaccag cagaaggtca tcgttgcaag gtggctgctt acccagccgg atattctgat
      961 tctcgatgaa ccgacccgcg gcatcgacgt tggtgccaag tacgagatct attcgatcat
     1021 cgcggacctg gcgaagagtg gcaagacgat catcatgatc tcctcggaga tgcccgaact
     1081 cattggaatg tccgaccgca tcatggtcat gtgtgaaggc cgggtcaccg gaatccttga
     1141 tggaaaagaa gcgacgcagg agcgcatcat gacgcttgcg acgcagctta tgtaacgggg
     1201 agacgaacat gaagaatcct gcatccacga gtatgagcaa gcgtgtcacg gcgcgttccg
     1261 tccgcgagtt cgcggtcagg aatgccatca tcctggtcct cgcggtattg gtcctggtca
     1321 tcggcattat caagaacggc ttcctgacac cgggcaacct tgtcaacatc atgaggatct
     1381 cctcggtccg tctcatcatt gcactcgcgt gcgccggcgc gcttattacg ggtggtactg
     1441 acctctcggc ggggcgcacc gtaggacttg cggcggtcat cgtcgcgtcg cttgtccagc
     1501 gcccggacta tgcaagcaag atcttcccga accttccata catgtgggtc atcttcccga
     1561 tcctgattgc ggttgctgca ggacttctcg tcggcatcat caacggtgtc gtcattgcct
     1621 atctgaaagt cccgcctttc atcgcaactc ttggcatgat ggtcgtcgtg tggggggctg
     1681 catcgctcta tgtcgacagg cctccgctgg gcgctcagcc gatcggtgga ctccggctcg
     1741 acttcacgaa cctggggaca gggtcgattg gtttcggacg cttcgcaatt ccgacgatca
     1801 tcctcatcgc gctgatcgtc ttcgccttct actgggtcat gctgaacaag acccagctcg
     1861 gcaagaacat gtatgcgatc ggtggcaacg tcaacgccgc catcgtctcg ggcgtcaacg
     1921 tcaagaggaa cctcgtggca gtgtacgcca ttgctggtac gctctatggc ctcgccggcg
     1981 tcctccttgc agcccgatcg ggcggtgcga cgaacaacta cggccagagc tatgagctcg
     2041 acgcgatcgc ggctgccgtc attggaggcg tctccacgag cggcggcaga ggaacggtcg
     2101 tcggtgtcct gacgggcgtc ctgatcttcg aggtcctcaa caacggtctc gtggtcctgg
     2161 gcgtttctgc gtattggcag caggtcgtca agggcatcat catcgtggcg gcggttgcgc
     2221 tggacatccg gaagtacagc aagagacagt agcccttgcg gctgcatcag ggaccattcc
     2281 gaaagatcgg agcccggcct gcaggccggg ctcttgcgtg tggccgcgaa caggcaaggt
     2341 ctggcctcag ctgacatcgt gcaggctcca tggctccccc atggctcgta tcatgtcggg
     2401 tccgtcgcga caccatccga agaccatgac cgccaccata tcgagattcc gctcagtgcg
     2461 ctcaggaagg cgtgcagtgg ccgatgtcgg ggc
//
LOCUS       DATMZD010000252         3349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_47217,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000252 DATMZD010000000
VERSION     DATMZD010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47217"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="VN478_05230"
     CDS             <1..263
                     /locus_tag="VN478_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22109.1"
                     /translation="TLAGAGVDPWWIYPSAPVKRDETGAMIPVMPCHEPFWELDNIVM
                     SPHTGGFTDVSVDGLWGESFENAIRADRGEAPKNLVDLEKGY"
     gene            431..937
                     /locus_tag="VN478_05235"
     CDS             431..937
                     /locus_tag="VN478_05235"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWQ22110.1"
                     /translation="MDEELTAVVARVHSGEEAAFDELYHLTVRQLYKTMYTYLLTRED
                     VEDAVQDAYLRLYQVRRKLDSTRSVPVYLRTIAINCAKRKLRRTHSAATPSEEGDVSD
                     TELMKGVVREALQQISPADRLVMGLFYGDQASIQDICRMTGEKEGTVKSRLSRARERL
                     REVIDHGT"
     gene            927..2666
                     /locus_tag="VN478_05240"
     CDS             927..2666
                     /locus_tag="VN478_05240"
                     /inference="COORDINATES: protein motif:HMM:NF039822.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HWQ22111.1"
                     /translation="MEHDDRKYTDMLNEGLKDVEPDAELHGKVREAITRNVARVRRRN
                     TAFGAVAIAIVLTVALSTVTPVFGRNGTLPQVVAAMAAQRQAQKMTETLGTATVQQTS
                     AALLVPEATVQTVADSTLSETDSILALVIADRVGKPVGEVVALRSQGLGWGVIMAQLG
                     ISGHDISEAVGRAQATAVASSSNTASQGQGTTSQPGVSNSNGDKIVVNGEITGVSTTL
                     ITVSARTFVIAPETQLKYHGKAITPAEIVAKLSAGKLYATVQGTRMADTSLRAALIIV
                     QDGADTQDTPSAENNDDQANQAVSNEQQARGKVTAVSATMLRIDGFAHDIVLNTATKV
                     EQVGAGKADVTSIKVGQTLQVHVVLSGTTYTAQQVHIEDKYVKADTSSDVEGSQPATP
                     SNERAAVSNEQQARGKVTAVSATMLRIDGFAHDIVLNASTKVEQVGAGKADVTFIKVG
                     QTLQVHVVLSGTTYTAQQVHIEDKYVKAGTNDEQTTDPALKTWQGTIASIVPGGQFIT
                     LAEVNPPYVFRIIVASVLQDSKNKSIAFASLAVGQKVTITGRLLADGSAEIVKLVVTT
                     DAGANPGKGNGKH"
     gene            2837..>3349
                     /locus_tag="VN478_05245"
     CDS             2837..>3349
                     /locus_tag="VN478_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWQ22112.1"
                     /translation="MDTVTRKSLIYRTGIGGAGAYAATHVVGCSHGCRFPCYAFLMMR
                     RFGKVATYEEWCQPRLVANALQLAQRELPRLRGKARSVHLSFATDAFMYQHPEVPALT
                     LQLMRLINSYDIPVHTLTKGVIPDEALHLSSRNQFGITLVSIDEEFRKRYEPGTAPYS
                     ERVAGLRRAHD"
BASE COUNT          731 a         1007 c         1043 g          568 t
ORIGIN      
        1 ggacactggc tggcgccgga gtcgacccat ggtggatcta cccgtcggcg ccggtgaagc
       61 gggatgagac gggggcaatg atccccgtca tgccctgtca cgagccattc tgggaactcg
      121 acaacatcgt gatgtctccc catactggcg ggttcaccga cgtctctgtc gacggactgt
      181 ggggcgagtc gttcgagaat gccatccgcg ccgaccgcgg cgaagccccc aagaacctgg
      241 tcgatctgga gaagggatac tgatagcctg accgccgccc tcaggctgca cggcgggcga
      301 cttgggagac gacacttccg gccccggtcc ctggatcggg gtcattccca tgtccgggca
      361 tgcaaccttt cggcgtggac tgggctatca ttgtggaaag gcatgagcgc cgatgcgaag
      421 ggatggtccc atggacgaag agctcacggc agttgtcgca cgtgtccact caggagagga
      481 agcagcgttt gacgagctgt atcacctgac ggttcgacaa ctctacaaga cgatgtacac
      541 gtatctgctt acgcgtgagg atgtggaaga tgcagtccag gacgcatacc tgagactcta
      601 ccaggtgcgc aggaagctgg acagtacgcg gtcggtacca gtctacctga ggacgatcgc
      661 catcaactgc gcgaagcgga agcttcggcg gacgcattcc gccgccacgc cgagcgagga
      721 aggcgacgtt tcggatacag agctcatgaa gggtgtggta cgagaagcat tgcagcagat
      781 ctcgccggca gatcgccttg tgatggggct gttttacggc gatcaggcct ccatccagga
      841 catctgcagg atgacgggcg agaaggaagg aacggtcaag tcccggctct cacgagcacg
      901 ggaacgacta cgggaggtaa tcgaccatgg aacatgacga tagaaagtac acagacatgc
      961 tgaacgaggg actcaaggat gtcgaaccgg acgctgaact gcacggcaaa gtgcgcgaag
     1021 cgatcacacg gaacgtcgcg cgggttcgcc gccgcaatac ggctttcggg gccgtggcta
     1081 ttgccatcgt cctgaccgtg gcgctgagta ccgtgacgcc ggtctttggc cggaacggga
     1141 cactccccca ggtcgtcgct gcaatggccg cgcagcggca ggctcaaaag atgaccgaga
     1201 ctctcggaac ggcaactgtg cagcagacga gtgccgcgtt gctggtgcca gaggcgaccg
     1261 ttcagactgt cgccgacagt acgctctccg aaacagacag catcctggca cttgtcatag
     1321 ccgatcgcgt tggcaagccg gttggagagg ttgttgccct cagaagccag gggcttggct
     1381 ggggcgtcat catggcgcaa ctcggcatct ctggacacga tatcagtgaa gcggtgggcc
     1441 gggcacaggc aaccgccgtg gcaagctcgt ccaatactgc ctcacagggg cagggaacaa
     1501 cttcacagcc cggcgtcagc aacagcaacg gagacaagat cgttgtcaat ggcgagatca
     1561 cgggcgtctc gaccacgctg atcacggtgt ccgccaggac attcgtcatc gcacccgaaa
     1621 cacagctcaa gtatcatggc aaggcgatca cgccggccga aatagttgcg aagctgtctg
     1681 cggggaagct ctacgctacc gtgcagggga ccaggatggc cgatacgagc ctgcgcgccg
     1741 ccctgatcat cgtccaggat ggagcggata cccaggacac accatctgcc gagaacaatg
     1801 acgaccaggc gaaccaggcc gtcagcaacg aacagcaggc gcgtggcaag gtcacagcgg
     1861 taagcgccac gatgctgcgc atcgacgggt ttgcccacga catcgtcttg aatacggcca
     1921 ccaaggtgga acaggtcggc gccggaaagg cagacgtcac gtccatcaag gttggacaaa
     1981 cccttcaagt gcacgttgtg ctctcgggta cgacctacac ggcccagcag gtgcacatcg
     2041 aggacaagta cgtgaaggct gatacgtcat ctgacgtgga aggcagtcag ccggccactc
     2101 ctagcaacga gcgcgcagcc gttagcaacg aacagcaggc gcgtggcaag gtcacggcgg
     2161 taagcgccac gatgctgcgc atcgacgggt ttgcccacga catcgtcctg aacgcgtcca
     2221 ctaaggtgga acaggtcggc gctggaaagg cagacgtcac gttcatcaag gtcggacaga
     2281 cgctccaggt acacgtggtt ctctcaggta cgacgtacac ggcgcaacag gtccacatcg
     2341 aggacaagta cgtcaaggca ggcacaaacg acgaacagac gacggacccc gccctgaaaa
     2401 cgtggcaggg cacgattgcc tccatcgtcc ccggcggaca gttcatcacg cttgcagagg
     2461 tcaacccgcc ttacgtgttc cgcatcattg tggcatctgt cctgcaggac tcgaagaaca
     2521 agtcgatagc attcgcgagt ctggcggtcg gtcagaaggt caccattacg ggcagactgc
     2581 tggccgacgg ctcagcagag atcgtgaaac tcgtcgtgac aactgacgcc ggcgcaaacc
     2641 ccggcaaggg caacggcaag cactaggact cgtcagccat tttccccctt ggcccgcagc
     2701 ttgccctgcg ggcttttcca ttagcccccg gcctgcggtc ctgcagaatc ctcctgccgt
     2761 gatcaccggg ctcgccacgc acagagggaa cgtttgccgc tcttgtccag ggcgtcgcga
     2821 aaggtaccat gtgagcatgg atacggtcac tcgcaagtcg ctgatctatc ggacgggcat
     2881 cgggggcgcc ggcgcctatg cggcgacgca cgtcgtcggg tgctcacacg gctgccggtt
     2941 cccgtgctat gccttcctca tgatgcggcg gtttggaaag gtggcgacat acgaggagtg
     3001 gtgtcagcca cgcctggtcg ccaatgccct ccagctcgct cagagggaac tgccacggtt
     3061 gcgcggcaag gcgcgctcag tgcacctctc gtttgcgacc gacgccttca tgtaccagca
     3121 tccggaggtc ccggcattga cacttcagct catgcgtctc atcaactcct atgacatccc
     3181 cgtgcacacg ctgaccaagg gggtcatccc ggatgaagcg ctgcacctgt cgagccggaa
     3241 ccagttcggc atcacgctgg tttccatcga cgaagagttc cggaaacggt acgaaccagg
     3301 cacggctccc tattccgaaa gggttgccgg cctgcgtcgg gcccatgac
//
LOCUS       DATMZD010000253         2276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_107830,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000253 DATMZD010000000
VERSION     DATMZD010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107830"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..412
                     /locus_tag="VN478_05250"
     CDS             167..412
                     /locus_tag="VN478_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22113.1"
                     /translation="MNEDNMMRKAMRPIEKNRRTRSKPAKIRHLQKQQKLWMVSKVEW
                     RKPGTKPGLAPSSFLLRTRLVPQRCLCTIDPWSRRAE"
     gene            402..1151
                     /locus_tag="VN478_05255"
     CDS             402..1151
                     /locus_tag="VN478_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22114.1"
                     /translation="MLSDQSPAQCPVCVETARDVSVLRQAGGFLVTACDTLGAIGSKP
                     GDVVVSPEEVCGSYTLRVCLSELVAVGASPLLVMSLVCNEWEPTGRKVLSAIRAELDE
                     NGYPDIAVTGSTEENMTTTMTALGIAVVGFAASMRWRRTVAGDRLVVVGFPRVGADVL
                     AHAGELLRPSIVQDLIRRDTVGDVIPCGSRGIRHELDVLAHEVGLRMAVREDVRIDLD
                     TSAGPATCALVTVHGSLEDCGLTATEIGVVE"
     gene            1156..1836
                     /gene="cbiR"
                     /locus_tag="VN478_05260"
     CDS             1156..1836
                     /gene="cbiR"
                     /locus_tag="VN478_05260"
                     /inference="COORDINATES: protein motif:HMM:NF041277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobamide remodeling phosphodiesterase CbiR"
                     /protein_id="HWQ22115.1"
                     /translation="MILGATSWQVPGSYLDNARLLAEHAAFAELLVYTWNDEMQTTLE
                     QQWDEMDGLLGLSVHLPADTLENAEGALRFLSGRNVLRYTVHPVDDAPALRRFLVSAV
                     ELVGDRLCLENLENDVFESVMPRVEDIPFSITMDYGHLLFAHTSAKGFVDAWGPRVQE
                     IHFHGYDGTDCHVEPDEGTLSDFGRLMDSQFGNRADVPVCVETFDWSVTLSILKALAP
                     HTAAIAGG"
     gene            complement(1847..>2276)
                     /locus_tag="VN478_05265"
     CDS             complement(1847..>2276)
                     /locus_tag="VN478_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008509006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWQ22116.1"
                     /translation="VEAVERAARAANAHDFIMRLANGYQTVLEESGKNLSGGEKQRVA
                     IARAILQNAPVLLLDEATSALDTESEQQVQEAIEHLMKGRTTLVIAHRLSTIQGADTI
                     YYLEDGRLVEQGTHQDLMSDPQSKYRALVEAGLRTTAGQV"
BASE COUNT          412 a          671 c          723 g          470 t
ORIGIN      
        1 cgcggcagat tcgagtcctg agaacgctgc cggagagggg ttggagctca gtaccgcctg
       61 ggaccggcag catcgaccct tcacgcccgg agcttgacat tggagtctcc cccgatacat
      121 ttatacggct tcatttcgac gagtgttgac gacaaggagg tggctcatga acgaagacaa
      181 catgatgaga aaggccatgc ggcccatcga gaagaaccgt cggacgcgga gcaagcccgc
      241 gaagatccga caccttcaga agcagcagaa gctgtggatg gtgtccaaag tggagtggcg
      301 caagcccggc accaagccag gacttgcgcc ttcatcattt ctgcttcgta cacggttggt
      361 ccctcagcgg tgcctctgca cgatcgatcc gtggagtcgc cgtgctgagt gaccagtcgc
      421 ctgcgcagtg tcccgtctgt gtcgagactg ctcgtgatgt cagcgtcctc cgacaagcag
      481 gagggttcct ggtcacagca tgcgacacgc ttggtgccat cggatcgaag ccgggtgatg
      541 tcgttgtctc tcctgaggag gtctgcggat cctatacgct ccgcgtatgt ctgagcgaac
      601 ttgtcgcagt cggtgcgtct ccccttctcg tcatgtcgct tgtctgcaat gagtgggagc
      661 cgaccggcag gaaggtcctg agcgccatca gagccgaact cgacgagaac ggctacccgg
      721 acatcgccgt caccggtagt accgaggaga acatgacgac gaccatgaca gctctgggga
      781 tagctgtcgt cggctttgcg gcctcgatgc gatggcggcg taccgtggcc ggggaccgcc
      841 tcgtggtggt cgggtttccc cgggttgggg ccgacgtcct tgctcacgca ggcgaactcc
      901 tcagaccatc catcgtccag gatctcattc ggcgtgacac agtgggagac gtcatcccat
      961 gcggctcgcg tggcatccgg catgagctgg acgtgcttgc gcacgaggtg ggcttgagaa
     1021 tggccgtccg agaagatgtt cgaatcgacc tcgacacatc ggcgggcccg gcgacctgcg
     1081 cgctggtgac ggtacatggc agtctcgagg attgcggcct gacggcgacc gagatcggcg
     1141 tggtcgaata gccccatgat cctgggggcg acgtcctggc aggtacccgg ttcatacctg
     1201 gacaacgcgc gtctgctggc cgagcatgct gcgtttgcgg aactgctggt ctacacgtgg
     1261 aacgacgaga tgcagaccac gctggaacag caatgggacg aaatggacgg tctgctcgga
     1321 ctatccgttc atctgcctgc agatactctt gagaacgctg aaggggcact gcgattcctc
     1381 tccggacgga atgtcctgcg ctacacggtc catcctgtcg acgacgcgcc tgctcttcgc
     1441 aggttcctgg tgtcggccgt ggagctggtc ggcgaccgtc tgtgcctgga gaacctggag
     1501 aatgacgtgt tcgagtctgt gatgccgcgg gtcgaggaca ttccgttctc gatcacgatg
     1561 gactacgggc atctcctttt cgcgcacacg tccgcgaaag ggttcgttga cgcatgggga
     1621 ccgcgagttc aggagatcca cttccatggg tatgacggga cggactgcca tgtcgagccc
     1681 gatgagggga cactgtccga ttttggcaga ctcatggaca gtcagttcgg caaccgcgcg
     1741 gacgtgccgg tgtgcgtcga gacattcgac tggagcgtca cgctcagcat tctcaaggcc
     1801 ctggcgccgc acaccgccgc tatcgccggc ggctgagcac gctggcctac acttgtccgg
     1861 cggtcgtacg caggccggcc tcgacaaggg ctctgtactt gctctgtgga tctgacatca
     1921 ggtcctgatg ggtaccctgc tccacgaggc ggccgtcttc caggtagtag atggtatctg
     1981 ccccctggat cgtagacagc cggtgagcta tcaccagcgt cgtacgtccc ttcatgaggt
     2041 gctcgatagc ttcctggacc tgctgttcgc tttcagtgtc gagtgccgaa gtggcctcgt
     2101 cgagcagcag tacgggtgcg ttctgaagga tcgcccgcgc aattgcgacg cgctgcttct
     2161 cgccaccact gagattcttg cctgactcct cgagcacggt ctggtatccg ttggctagcc
     2221 gcatgatgaa gtcgtgagca tttgcagctc tggcagctct ctcgacagct tccacg
//
LOCUS       DATMZD010000254         3630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_138602,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000254 DATMZD010000000
VERSION     DATMZD010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138602"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /locus_tag="VN478_05270"
     CDS             <1..957
                     /locus_tag="VN478_05270"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HWQ22117.1"
                     /translation="DMASASIKLNEDGTYFLAMGATDIGTGSDTILSQMAAEVLGVTV
                     DKISALSSDTDLTPFDVGAYASSTTFVSGGAVVKAAKAVRNQIFSVAAEMLGEELDQQ
                     LVIAEPKVEWDNDQPDPILAFYRMEEGNVVSVRSGKKVSVSDIAYRAYYEVNQKQIAT
                     TQSFVSPYSPPPFAAHFAEIEVDELTGKVEVLKYVAAVDCGVSINPDLVRGQVMGAIA
                     NGIGFALTEEYHFGSHGNMTNSSLFDYKILSRRDMPPTEVIVVDNYEPTGPWGAKSVA
                     EIGINGPIPAIGNAIFDATGIRLHRTPYTPERMLAALHSKTV"
     gene            complement(1032..2744)
                     /gene="pepT"
                     /locus_tag="VN478_05275"
     CDS             complement(1032..2744)
                     /gene="pepT"
                     /locus_tag="VN478_05275"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: protein motif:HMM:NF003976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HWQ22118.1"
                     /translation="MTENEELVRTTIRSGEATLCAVADGALVYQLTGRGIGALVTAVE
                     KERAASHGALDWGDKLVGRAAALLFTLVRPRSVFALTMSTGAQDVLRAADIPFTCDEV
                     IVDVLNRAGTGPCPMEAAVSSIDDPLAALEKLKQVSQSLSNAGRNHAPEKGESMNNLV
                     DRFLHYTSFDTQSDEHSVTFPSTEKQLVLAHALDAELQALGLKDSSVDANGYVTATLP
                     ANCDNPGAVPVIAFLAHMDTSPDASGMGVKPRIVTIYDGQDIILNAEKNMVLSPVDFP
                     DMLLHKGEDFIVADGTTLLGADDKAGIAEIMTAIEYLLAHPEVKHGVIKVGFTPDEEV
                     GQGVDRFDVAKFGAKYAYTLDGDELGSLEYENFNAASAEFEITGKSIHPGAAKGIMKN
                     AALIAAELTTLFPSDETPRSTEKREGFFHLTSISGMCEKAEAKYIIRDHDKQRFEQRK
                     SMAHAVCDFLNAKYGEDTVTVHVRDSYYNMREVIEQNMILVDTARGVMEKLGVTPLVK
                     PVRGGTDGSRLSYMGLPCPNLFTGGRNYHGPLEYIVVQSMEKSVQVIVGIAEAFASQP
                     PAAL"
     gene            2923..>3630
                     /locus_tag="VN478_05280"
     CDS             2923..>3630
                     /locus_tag="VN478_05280"
                     /inference="COORDINATES: protein motif:HMM:NF037962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese transporter"
                     /protein_id="HWQ22119.1"
                     /translation="MANVALDALNDTLRMAPILLGVYLLLEYVEARWQQRIIDAVERA
                     GRVGPVVAAAGGAIPQCGFSVMASALYTQRLLTMGTIVAVYLATSDEALPILLSKPDK
                     AHVVFPLIGIKVLLAVAGGYLVDAFMLSENRRRTQECRDRRTAEQDAVRIGEESCSCD
                     GTRASCTSKETGGVDWKEILWSATKRTAQIVLFVFATSLVIGLLINRIGQENLSRVFL
                     THTVFQPVLVSLLGLIPN"
BASE COUNT          681 a         1053 c         1114 g          782 t
ORIGIN      
        1 gacatggctt ctgcttccat caagctcaac gaggacggga cctacttcct ggccatgggg
       61 gcaacggaca tcggtactgg ttccgatacc attctgtcgc agatggcggc tgaagtcctg
      121 ggggtcaccg tcgacaagat cagtgcgctc tccagcgata ccgacctgac gccgttcgat
      181 gtcggggctt atgcctcatc gaccacgttc gtctcaggcg gcgccgttgt caaggcggcc
      241 aaggcagtcc ggaaccagat cttctcagtc gcagccgaga tgctgggcga ggaactggac
      301 cagcagctgg tcattgccga gcccaaggtg gaatgggaca acgatcagcc ggaccccatc
      361 cttgccttct atcgtatgga ggaggggaac gtggtatcgg tgcggagcgg aaagaaagtc
      421 tccgtgtctg acattgccta tcgtgcctac tacgaagtca accagaagca gatcgcgacg
      481 acgcagtcgt tcgtgtcccc atactctccg ccgcccttcg cagcgcactt tgcagagatc
      541 gaagtggacg agctcaccgg gaaggtcgag gttctcaagt atgtggcagc cgtcgactgc
      601 ggcgtgtcca tcaaccccga cctggtacgt gggcaggtga tgggtgcgat agcaaacggc
      661 attgggtttg cgttgaccga ggagtaccac tttggatccc acggcaacat gaccaattcc
      721 tcgctgttcg actacaagat cctgtctcgc cgtgacatgc cgccgacgga ggtgatcgtc
      781 gtcgacaact acgagccgac ggggccatgg ggcgccaagt cggtggccga gatcggtatc
      841 aatggaccta tccccgcaat cggcaatgcg atcttcgatg cgaccgggat tcgcctccac
      901 cgtacgccgt atacgccgga gagaatgctg gccgcactgc acagcaagac cgtctgacca
      961 ggatccggaa acaggaacac aaaggggcgc ccaagagggc gccccttttc catgtttgtg
     1021 gcaggcttgc gttacagtgc agcaggtggc tggctcgcaa acgcctcggc aatgcccacg
     1081 atgacctgca cggatttctc catggactgg acgacgatgt attcaagggg gccgtggtag
     1141 ttgcggccgc cggtgaacag gttagggcag ggaagaccca tgtagctcag gcgtgaaccg
     1201 tcggtcccgc cgcgaacagg cttgacgagg ggagtgacgc cgagtttctc catgacgccg
     1261 cgagctgtgt ccacgaggat catgttctgc tcgatgactt cgcgcatgtt gtagtacgag
     1321 tcgcgcacgt gaacggtaac cgtgtcctcg ccgtacttgg cgttcaggaa gtcgcagaca
     1381 gcatgcgcca tactcttccg ctgctcgaag cgctgcttgt catggtcgcg gatgatgtac
     1441 ttggcttcgg ctttttcgca catgccactg atgctggtca ggtggaagaa gccttcgcgc
     1501 ttctcagtcg agcgaggagt ctcgtcggac gggaacagcg ttgtcagttc cgctgctatc
     1561 agagctgcgt tcttcatgat accctttgcg gcgcccggat ggatgctctt gcccgtgatc
     1621 tcgaattctg ctgaggcggc attgaagttc tcatattcca ggctgccgag ctcgtcgccg
     1681 tcgagcgtgt aggcgtactt ggcgccgaac ttcgctacgt cgaaccggtc gacaccctga
     1741 ccgacttctt cgtccggcgt gaagccgacc ttgatgacgc cgtgcttcac ctcgggatgt
     1801 gccagcaggt actcgatggc agtcatgatc tctgcgatac ctgccttgtc gtcggccccc
     1861 agcaacgtcg tgccatcagc gacaatgaag tcctcgccct tgtgcagcag catgtcggga
     1921 aagtcgacgg gactcaggac catgttcttc tcagcattga ggataatgtc ctggccgtca
     1981 tagatcgtga cgatacgcgg cttcacgccc atacccgacg cgtcaggaga cgtatccatg
     2041 tgcgcgagga atgcgatgac aggaaccgcg ccggggttgt cacagttggc gggaagggtc
     2101 gcggtgacat agccgttcgc atcgacagaa gaatccttca gaccaagtgc ttgcagctct
     2161 gcgtccagcg catgggcaag gacgagttgt ttctcggtgc tgggaaacgt gactgagtgt
     2221 tcgtccgact gcgtgtcgaa tgacgtgtag tgcaggaatc gatccaccag gttgttcatt
     2281 gactccccct tctcgggcgc atgattgcgc ccagcatttg atagtgactg actcacttgc
     2341 ttgagcttct ccagtgcggc cagcgggtcg tcgatactgg ataccgctgc ctccatcgga
     2401 cacggtcctg tgccggccct gttgagcaca tcgacgatga cttcgtcgca cgtgaatgga
     2461 atatcggctg cgcgcaggac gtcctgcgca ccagtgctca tcgtcagggc gaagaccgat
     2521 ctgggacgca ccagcgtgaa caggagtgca gcggcccgtc ccaccagttt gtctccccag
     2581 tccagggcgc catggctggc agcacgttcc ttctcgactg cggtgacaag cgcgccgatc
     2641 cctctgcccg tcagttggta gacgagtgct ccgtcagcaa cggcgcagag cgtcgcttct
     2701 cccgacctga tagttgtccg tacgagttct tcgttttccg tcatgagctc ctcgtttcgc
     2761 cgcctcggaa gcggcacggt tcattgtatc cgaaactgac cggtcgtaca gcttcctgga
     2821 tacgtggctc cctcactgtc tgcccgttga tgtcgcagca cgggggctat aatatcaagg
     2881 gtcttgctgc aaatcatttg catgtagaag ggtggtcggt cgatggcgaa tgttgctctg
     2941 gacgctctca atgatactct caggatggcg ccgattctgc tcggggtcta cctgctcctg
     3001 gaatacgtcg aggccaggtg gcagcagagg atcatcgacg cggtcgagcg agcggggcgt
     3061 gtcgggcctg ttgttgcagc ggcggggggc gccattcctc agtgtggctt ctcggtcatg
     3121 gcaagcgcat tgtacacgca gcgactcctc acgatgggca cgatcgttgc cgtgtacctt
     3181 gccacatctg acgaagccct gcccatcctt ctttcgaagc cggacaaggc gcatgttgtg
     3241 tttcccctta tcggcatcaa ggtgttgctt gctgtcgctg gaggatacct cgtcgacgcg
     3301 ttcatgctgt ccgagaaccg caggcgcacg caggagtgcc gggaccgcag gactgccgag
     3361 caggatgcgg tgaggatcgg cgaggagagc tgttcctgcg acggcacgcg agcatcgtgc
     3421 acgagcaagg agaccggcgg cgtcgactgg aaggagatcc tgtggtcggc aacgaagcgg
     3481 acggctcaga tcgtcctgtt tgtctttgcg acgtcgctgg tcatagggct gctcatcaat
     3541 cgtatcgggc aggagaacct gtcgcgcgta ttcctgaccc acacggtctt ccagcctgtt
     3601 ctggtgtccc ttcttgggct cattccgaac
//
LOCUS       DATMZD010000255         3995 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_138662,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000255 DATMZD010000000
VERSION     DATMZD010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3995
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138662"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1302
                     /locus_tag="VN478_05285"
     CDS             <1..1302
                     /locus_tag="VN478_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22120.1"
                     /translation="DMDPGSSVRELVYAIENHSTTWTLPSTARWVNSGDLVPLALATP
                     GMTRVVKEWFEWVNATDRSLRVPWYEAGWMNSAGTWMVQQLARFDSRPTGPVEQVCSW
                     QRSCVLKLPATHGVAYFKAVPVVLGSEVEISVLLDHEYPGMVAEVMAVDGDRRWLLMR
                     DMGAETLDSIPDVERWKTAVHAYAQLQVDWVDAARRFAIAGCPNRGLLQLVSSIDRLL
                     ADTVAMKPGAADGLSMKQIHALHYRARALKIAAHRLGTLRLPVSVDHGDFWAGQIVAG
                     DSNPVFIDWSDASLSHPFFSMAFFSDEEEMRPYLGDVPGAADQLRDAYLEPWTVYEPH
                     DILTQAWSIACSLAPLSTALLYHDRILPGMEVKWEMENMIPYFLHSVLRHEGEALPTL
                     GMADYEQQAESSEFEQEDLRSSDGTVALRSQLQARQIAGPH"
     gene            1502..1843
                     /locus_tag="VN478_05290"
     CDS             1502..1843
                     /locus_tag="VN478_05290"
                     /inference="COORDINATES: protein motif:HMM:NF024087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HWQ22121.1"
                     /translation="MEMIGACGLVCSTCPAYVAGTTGDTALQEKTAAAWSAAYGTEVK
                     PEDVACDGCQSTGPRLFSHCSVCDVRLCARGKGYATCAECGEYGECKTINGFFGFCPE
                     AKPALDALRAK"
     gene            1935..2840
                     /locus_tag="VN478_05295"
     CDS             1935..2840
                     /locus_tag="VN478_05295"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWQ22122.1"
                     /translation="MILAARLSAEQVSHVRALADLCRSRDGYSMEARLDFGTLETRPD
                     GSVVDVLWYEGPQLVGFAGQSSYGDPTEVETSILVHPDFRRRGMGRAITSVVVRECQN
                     HGVTQLLYVVPRESTEGAGFAAAMGTQHSHSEYTMDLDASPVPASSATSDPVELRPAG
                     AEDIPVMAAIVASAFSSPVQKEEEALARLMRDSTRTTYLATKDGRPVGVIQSAVSDGR
                     AFIVHFAVRPDMQGHGIGRQMLTAIVHGLLNGGTRWVTIEVETENSHALSLYQACGFA
                     TVNATDYELLQLPRIPVAPELLFDR"
     gene            complement(2841..3716)
                     /locus_tag="VN478_05300"
     CDS             complement(2841..3716)
                     /locus_tag="VN478_05300"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015346485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWQ22123.1"
                     /translation="MEPSRPMAGKVCLVTGATRGLGAVTAEALARMGATVVAAGRSAE
                     RGQDLVSGVSQRTGNRDVHFLCADLASMANVRGLAHAFTDRWDRLHVLVDNAGTVFPT
                     RRITVDGYEEVFAVNHLAPFLLTSLLLPVLRASAPSRVIVTSSYEHRRARMHWDNLQG
                     ERFYFSQQAYAQSKLANLLFVYELARRLQGSGVTANAVNPGLVHTNICMDSGWLIGTV
                     KRMMDRFSGLTPEQGADTGVWLASSPDVEGISGRYFQRRHAVRSSRASYDVAAQERLW
                     QISEVLTGLHSRPGA"
     gene            complement(3725..3995)
                     /locus_tag="VN478_05305"
                     /pseudo
     CDS             complement(3725..3995)
                     /locus_tag="VN478_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010997873.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="polynucleotide kinase-phosphatase"
BASE COUNT          711 a         1227 c         1338 g          719 t
ORIGIN      
        1 gacatggacc ctggttcctc tgtcagggaa cttgtctacg cgatcgagaa tcactcgacg
       61 acctggacgc tgccctcgac cgctcgctgg gtgaactcgg gggacctcgt tccccttgct
      121 ctggcaactc ccggcatgac acgcgtcgtc aaggagtggt tcgagtgggt caacgccaca
      181 gacaggtcac tgcgagttcc atggtacgag gccggatgga tgaactcagc cggcacatgg
      241 atggtacagc agcttgccag gttcgacagc aggccgaccg gaccggtgga acaggtctgt
      301 tcctggcagc gctcctgcgt gctgaagctg ccggcaacgc acggggtggc atacttcaag
      361 gctgtccccg ttgttctggg cagtgaggtc gagatcagtg tcctgctgga ccatgagtat
      421 cctggcatgg tcgccgaggt aatggctgtc gatggggacc ggcggtggct gctgatgcgg
      481 gacatgggtg ccgagacgct ggacagcata ccggacgtcg agcgatggaa gacggcagta
      541 catgcctacg cgcagctcca ggtggactgg gtggacgcgg ctcgcagatt cgcgatcgcg
      601 ggctgtccca accggggcct gctgcaactg gtatcgtcca tagaccgcct gctggcggac
      661 accgtggcca tgaaaccggg tgccgccgat ggactgtcca tgaagcagat ccatgcactg
      721 cactaccgtg cgcgggctct gaagatcgcg gctcatcgtc tgggcacgct gcgcctgccc
      781 gtcagcgtcg atcatggcga cttctgggcc ggtcagatcg tcgccggcga ttccaaccca
      841 gtgttcatcg actggtctga tgcgtccctg tcgcatccat tcttctcgat ggccttcttc
      901 tcggacgaag aggagatgcg cccgtacctt ggggacgtcc ccggtgcggc cgatcagcta
      961 cgggacgcgt acctcgagcc atggacggtc tacgagccgc atgacatcct gacacaggcg
     1021 tggagcattg cctgttcact ggcgcccctg agcaccgcgc tgctctacca cgaccgcatc
     1081 cttcccggga tggaggtgaa gtgggagatg gagaacatga tcccctactt cctgcacagc
     1141 gtgttgcgcc atgagggcga agcgctgccg acgctgggca tggcagacta cgagcagcag
     1201 gcagagtcgt cggagttcga acaggaggac ctgcgctcgt ctgatggtac ggttgccctg
     1261 cgttcccagc tgcaggcgcg gcagatcgcg ggaccgcact gagccagggg agacggtcag
     1321 tccttcaggc cctggacgac gagcggattc cacttggttc catgcgacgg gcagacgtgg
     1381 ctggttctgt ccatctggcc attctgtgat gaggtcgctt atgccgcgtt tggccgtata
     1441 ctaagctgga acctggacac gttatgtctc aagagcagct ctacggacaa aggagatggc
     1501 tatggagatg atcggagcgt gtggtcttgt ctgttcaacc tgcccagcct acgtcgcagg
     1561 aacgaccggc gacacagcgc tgcaggagaa gacggccgcg gcatggtcgg cggcctacgg
     1621 taccgaggtc aagcctgagg acgtggcctg cgacgggtgc cagtcgacag gacctcgact
     1681 gttctcgcac tgcagtgtct gcgacgtgcg gctatgcgcg cgcggcaaag gatatgccac
     1741 ttgtgctgag tgtggcgagt acggcgagtg caagacgatc aatggattct tcgggttctg
     1801 ccccgaagcg aaacccgccc tggacgccct gagagcgaag tagtcggtct gctgcctgca
     1861 ttcccgaact ggcacacggg cctcgtagac tcgcgaggtg gcaggatggg tcaactgccg
     1921 actgaggcaa cggcatgatc ctggcggcga ggctctcggc ggagcaggtc agccacgtca
     1981 gggcactcgc cgacctgtgc cgatcgaggg acggatactc catggaggcc cggctggact
     2041 tcggcaccct ggaaacgcgg cctgacggtt ccgtcgttga cgtgctctgg tacgagggtc
     2101 cacagcttgt cgggttcgcc ggtcagagta gctatggcga ccccacggag gtcgagacga
     2161 gtatcctcgt gcaccccgac ttccgcagga ggggcatggg acgggccatc acaagcgtcg
     2221 tcgtccggga atgtcagaat catggcgtga cacagcttct gtatgtcgtg cctcgggagt
     2281 cgacggaggg cgccgggttt gcggccgcaa tgggaacgca gcactcgcac agcgagtaca
     2341 caatggacct ggacgcgtcg ccggtccctg cgtcttcggc gacaagtgac ccggtggagc
     2401 tcaggcccgc aggcgccgag gacatcccgg tcatggcagc cattgtcgcg tccgccttct
     2461 ccagtccagt ccagaaagaa gaggaggcgc ttgcgcgcct gatgagggac tccacgcgta
     2521 ctacgtacct ggcgacgaag gatggacgac ccgtcggcgt catccagtcg gccgtcagcg
     2581 acgggcgtgc gttcatcgtc cacttcgcag tgcgaccaga catgcagggg cacggcattg
     2641 ggcggcagat gctgacggcc atcgtccacg gcctcctgaa cggcggcacg cgctgggtta
     2701 cgatcgaagt cgagacggag aacagccatg ccctctctct gtatcaggcg tgtgggttcg
     2761 caaccgtcaa cgcgaccgac tacgagttgc tgcaactgcc caggatcccc gtggctcccg
     2821 agctcctgtt cgaccgctag ctacgcccct ggtcgactgt gaagacctgt cagcacttcg
     2881 ctgatctgcc atagacgttc ctgggcggcc acatcgtagg aggcgcgcga cgatcggact
     2941 gcgtgtctgc gctggaagta gcggccgctg atcccttcga cgtcaggaga tgatgccagc
     3001 cagacgcccg tgtctgcgcc ctgctccggg gtcagcccgg agaaccggtc catcatccgc
     3061 ttgaccgttc cgatgagcca tccgctgtcc atgcagatgt tcgtgtgtac caggccgggg
     3121 ttgacggcat tggcggtgac gccgcttccc tgcaggcgcc tggccagttc gtagacgaac
     3181 agcaggtttg ccagcttgga ctgcgcatat gcctgctggg agaagtagaa gcgctcgccc
     3241 tgcaggttgt cccagtgcat ccgcgcgcgc cggtgctcgt aggaggacgt cacgatgacg
     3301 cgcgacgggg cgctggcccg cagcacggga agcaggaggc tggtgagaag gaagggagca
     3361 aggtggttga ctgcaaagac ctcctcgtac ccgtcgactg tgattcgccg cgtcgggaag
     3421 acggttcccg cgttgtcgac caggacgtgc aggcggtccc accggtcagt gaatgcgtgg
     3481 gcgagaccac gcacgttggc catcgacgcg aggtcggcgc acaggaagtg cacgtcccgg
     3541 ttgccggtgc gctgactcac gccggacacg aggtcctgtc cgcgttcggc actgcggccg
     3601 gcggcgacga ccgtggctcc catgcgggca agagcctccg ccgtgaccgc gccgagaccg
     3661 cgtgtggcgc ccgtcaccag gcacaccttg cccgccatcg gccgggacgg ttccatagca
     3721 gctacagagg cttgatcggg gtggagtaga cgcgcgcggc aggcacgctg accagctcgc
     3781 gctcgggata gcgcatcgcc gtcaggcttc cgccgtggac gcagccggta tcaatgttga
     3841 tcgtgttccc gacccacacc gcgtcacgga ccggcagatg tccccagacg acgactgcgt
     3901 ctcccttgta gtcgtgagcc cagtctacca tgaccttgag cccgaagtca tcgcgccggc
     3961 cggtcggagt gccaaacagg gcgacgtcgc gtgac
//
LOCUS       DATMZD010000256         2514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_67461,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000256 DATMZD010000000
VERSION     DATMZD010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67461"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            216..974
                     /locus_tag="VN478_05310"
     CDS             216..974
                     /locus_tag="VN478_05310"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HWQ22124.1"
                     /translation="MKKRLVFAALQSVLIVAIAAVIAFSPAVAARQGVKADTTDTTPA
                     PSVSVTGTGKVTVKPNIARISFGVFTHDETANAAQTANDAVMAKITAALKAAGIKDED
                     IQTTGYSLQPRWVWDRTNEKNVQSGYDMNHNVTVTVHTIANAGKIVSLIADNGANTVD
                     GISFDVDDATLEQTRLTAIDLAMTNARRRADVVAKAAGRTILSVQTVTVNENSYVAYQ
                     SYRNEAIAASDSAAAPIEAGTTDVTVSVNVTYLF"
     gene            1126..1593
                     /locus_tag="VN478_05315"
     CDS             1126..1593
                     /locus_tag="VN478_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22125.1"
                     /translation="MATWLFRGNPRDFDVNAYLQAHRDIRWYVHQQLLIPEMHLGDPV
                     YIWRSDGGLPGTGGIVARGVLSGPAIVRPDVDLVTWLRRKPDISIPSVLIRLDDVRLT
                     PRSGCLLRREIMQDAILRNLEVISMPSVVNYKLTAVEDARLTQVWEGRRPRDL"
     gene            1695..2156
                     /locus_tag="VN478_05320"
     CDS             1695..2156
                     /locus_tag="VN478_05320"
                     /inference="COORDINATES: protein motif:HMM:NF013995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HWQ22126.1"
                     /translation="MATWLFQGSPKDFPAFDDYLRNYTEISWHVRQKSAAEEIYPDDE
                     VYIWRLDGNRPGTGGIVAHGVVTTEARVIPDEGKKSWVSHQPGPTVPSVDITLDDVRL
                     TPEAGCLTRTALLEDPILWNMHVVQSPHLTNYRLTPEEEERIATLWRAAKR"
     gene            complement(2176..2364)
                     /locus_tag="VN478_05325"
     CDS             complement(2176..2364)
                     /locus_tag="VN478_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22127.1"
                     /translation="MMNIGKPLILAIGTLVGLVSIVLSNRPGYERIPDVDGREYAVNR
                     WTGKVSVVDSDVDSRRPR"
BASE COUNT          537 a          804 c          750 g          423 t
ORIGIN      
        1 cgaaggactc gggggggtga cagagtcgac tacacaccag ccttcgtgcg aatagcgaag
       61 ccagtgcata ccatcctcag tcagaatggc gaagcctcgg gcgcccggca ttgggaactc
      121 tgagacagct gcgacgtcct agcagtgaac acgaaacgcg accggccgaa cgaaggcagg
      181 cgcacacttt gcttgtgtac tacaggaggt atcccatgaa gaagagactc gtttttgcag
      241 cactgcagtc cgtcctgatc gtggcgatag cagccgtcat cgccttctcc ccggccgtcg
      301 ccgcgaggca gggtgtcaag gctgacacca cggacaccac tcccgcaccg tccgtcagcg
      361 tcaccggcac cggcaaggtc accgtcaagc ccaacattgc gcgcatcagc tttggcgtct
      421 tcacgcatga cgagacggca aatgccgcgc agacggccaa cgacgccgtc atggccaaga
      481 tcaccgcagc cctcaaggcc gccggtatca aggatgagga catccagact accgggtaca
      541 gccttcagcc ccgatgggta tgggacagga ccaacgagaa gaacgtccag agcggctatg
      601 atatgaacca caacgtcacc gtcactgtcc ataccatcgc caacgccggc aagatcgtga
      661 gccttatcgc cgacaacggt gccaataccg tggacgggat ctccttcgac gtcgacgacg
      721 ccacgctcga gcagaccagg ctgaccgcca tcgacctcgc catgaccaac gcccgcagga
      781 gagcggacgt cgtcgccaag gcagccggca ggaccatcct ttcggtccag accgtgaccg
      841 tcaatgagaa cagctacgtt gcataccagt cgtacaggaa cgaggcaatc gctgcatcag
      901 acagtgccgc agccccgatc gaagccggga ccacggacgt gaccgtcagc gtaaacgtca
      961 cgtacctgtt ctagggttca catacattcg cactgaccag acccccgccc cgcgcggggg
     1021 tcttttcatg agcctggtcg cgagatacgc agggtctgtc ccatcttgca cagaacacct
     1081 gcccacatag actgaagatc agtgggatcg accaggaggc agcagatggc gacatggctt
     1141 tttcggggca acccgagaga cttcgacgtc aacgcgtatt tacaggcgca ccgtgatatc
     1201 cggtggtacg ttcatcagca gttgctgatc ccggagatgc acctgggtga ccccgtctac
     1261 atctggcggt ccgacggcgg tctgcccgga acggggggga tcgtcgcccg cggggtcctg
     1321 tccggcccgg ccatcgtgcg tcctgacgtc gacttggtca cgtggctgcg caggaagcca
     1381 gacatctcca tcccaagcgt cctcatccgg ctggacgacg tccggctgac gccccgctca
     1441 gggtgccttc tcaggaggga gatcatgcag gatgccatcc tgcgaaacct ggaggtcatc
     1501 agcatgccca gcgtggtcaa ctacaagctg acggccgtcg aagacgctcg cctgacgcag
     1561 gtgtgggagg ggcgcaggcc gcgcgacctg tagccggagt ggacgagtca gtcaggcaat
     1621 tgtgctcaag cctctcttga tgaacggaga cgacgacgta cagtacagac aggtatggac
     1681 atgggaggta cgacatggcg acgtggctgt tccaggggag tcccaaggac tttccggcct
     1741 tcgatgacta tcttcgcaac tacaccgaga tctcgtggca cgtgcggcag aagagtgcgg
     1801 cagaagagat ctacccggat gacgaggtat acatctggcg cctggacggc aaccggccgg
     1861 gcacaggcgg catcgtggca cacggggtcg tgacgacaga ggcccgcgtc atcccggacg
     1921 agggcaagaa gtcatgggtc agtcatcagc cgggcccaac cgtcccgtcg gtcgacatca
     1981 ctctcgacga cgtccgcctg acgcccgagg caggatgcct gacgcggact gccctcctgg
     2041 aagacccgat cctgtggaac atgcacgtcg tccagtcacc acacctcacc aactacaggc
     2101 tgactcctga agaggaggag cggatcgcca ccctctggcg cgcagcgaaa cgatagctgt
     2161 tgccggtcgc gagccttagc gaggcctccg gctgtcgaca tcggaatcga cgaccgagac
     2221 cttgcccgtc catctgttga cggcgtattc ccgcccatcg acgtccggga tgcgctcata
     2281 cccgggacgg ttgctcaaga cgatcgatac cagtccgacg agtgttccga ttgccaggat
     2341 caacggcttt cctatgttca tcatgagatc atccccctgt gcgctgctgg tccgggacca
     2401 tgcggtcctc gtctatcaag acgcagcccg ggctcctgag gttcccagcg aaccacatga
     2461 acagtgtcct ggagccgcgg cccgtcacat ggcatacgtc cgtcgtctgc gctg
//
LOCUS       DATMZD010000257         5096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_147862,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000257 DATMZD010000000
VERSION     DATMZD010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5096
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147862"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1963)
                     /gene="dnaE"
                     /locus_tag="VN478_05330"
     CDS             complement(<1..1963)
                     /gene="dnaE"
                     /locus_tag="VN478_05330"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HWQ22128.1"
                     /translation="MSFVHLHLHTGFSFHESPIRIEDLAQRSHELDYSSCAITDSNTL
                     AGAIRFDRAARSAGVTPVFGTELTLLSDEPDDHVVVLARDLEGYRNLCRLVTRAQAQG
                     RIHPGLTLEQLAPYAGHLIALYGSPESLLYRSLSTRRPCKARCLLEEMRKLFETSLYL
                     DVQNPLFTDSTRLLYRMRRLSDETGVPACATGGVTHLHRIDAEVREMLLAIGSLEDVD
                     ARSRRSHVNAENWFRGADEMQELFADWPEALSASGRIAAECQVELPLDGFKTPSSVLT
                     ATRSLRDIVYAGAREKYGIVTTAVEERIEHELGIIRHMHMDEYFLIVRDIIEIARRKD
                     VAYSGRGSGANSIVCYCIDATQVDPIQCNLLFERFLNEGRKSLPDVDIDFDALRRDEV
                     VSELFLVFKEDRVCRVANVNTYNIRGAFRDVGKALGYSKEEVDDATRLLPWYSHRRIS
                     DVIAKLPELATFPYGSIERWVSLAERIMGLPRTLSTHLAGLIIAPFPLTDLVPLEPSG
                     TGCLITQYDKDDVEHLGLVKMDLLGLRTLSAAVDATAMVRAETGTDLSLSTIPMDDQP
                     TYRLLREARTLGVFQLESPGMRNLLLKLQPVCYTDIMANVSLFRPGPMAMNMDKLYLA
                     RRNGKVPVTFEDPRLESVLKETYGVIIFQE"
     gene            complement(1988..2266)
                     /locus_tag="VN478_05335"
     CDS             complement(1988..2266)
                     /locus_tag="VN478_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22129.1"
                     /translation="MNPTSCFPVLPTPSDNLPLLYIEERGGHLSAFVFRGEVKHVDRV
                     LKMWASGRFTSGHTEDATYYTIMSDDKMIATVARDNVHRHWYMVRLMD"
     gene            complement(2276..2866)
                     /gene="lexA"
                     /locus_tag="VN478_05340"
     CDS             complement(2276..2866)
                     /gene="lexA"
                     /locus_tag="VN478_05340"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HWQ22130.1"
                     /translation="MMEELTGKQQAVLEFIEVRARQTGFRPSLEDVRRHFGFNSLNSV
                     VVYVKTLERKGYLPQPDLIKGTPATPSLMIPVLGTVAAGTPILAEENIEKWIFVTPPT
                     PATADYFGLKVKGDSMIGEHIVPGDIVVVHKQQTADNGDIVVALFGDEATVKKLHEGD
                     GGIFLMPANPAYHPIPVTSDVSIIGKVVGLVREHLS"
     gene            complement(3070..4518)
                     /locus_tag="VN478_05345"
     CDS             complement(3070..4518)
                     /locus_tag="VN478_05345"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HWQ22131.1"
                     /translation="MRSPGLENDELDCTTVIIGRSASQSGSLVVGHNEDDAHRLVVRH
                     HIVKAGEHPAATRLRDGTLLYREPHTSLELPVPAGTLAYDWTELVGQDFADSFLNCEG
                     VSICCDSAAGTREQQPELERGGIGHFLPQLVAEQAHSAREGVLIAGRLVEAYGYCDAR
                     IITIADQNEAWVLQIPGGHEWLAQRVPDDAVVVLPNYLISRKVDLANTDWFLASTDLE
                     AHAIQRAWYDSTGTEPFDFKEAYGPPEINVSSLSTSRQQTGLFLLTGHEFPLDDMPFS
                     CVPEHPVTIGDVMACLRCVTPHGQSTRQALAMSGSFHKNVARTTMRPISVWTTQESSV
                     TELRNQNNGPQSVTVWRASGIPDELPYTPWYPLAMMETGLGYPDPYATGDPDHLDMSS
                     AFWAFRLFVTAVDCDYAGHIAEVRAALLPLEAQAMDTDRLLQNVPADASRKALAGLLT
                     AYGEGLGMEALNICSELLRQWQMNSERTPFRE"
     gene            complement(4518..>5096)
                     /locus_tag="VN478_05350"
     CDS             complement(4518..>5096)
                     /locus_tag="VN478_05350"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWQ22132.1"
                     /translation="EIHCELLLNGSRHIPFASLSPELEQNSIVCMAPSKTFNLAGLAT
                     SFIVVPNPRMRAAMQRRISGAVGSVTPFGYTALEASFREGDDWLTQLLHYIEGNVTYV
                     RLFVQDHMPQIKVMPIEGTYLMWLDFRALGMDIHQLSDFLRKKAKVGLDEGYVFGPGG
                     EGFARMNIACPRSTVQEGLTRIAAALQTLELR"
BASE COUNT         1016 a         1408 c         1585 g         1087 t
ORIGIN      
        1 gttcctggaa gatgatgacg ccataggtct ctttcagaac cgactccagt cgtgggtctt
       61 caaacgttac cgggaccttg ccgtttctcc tggcaaggta cagcttgtcc atgttcattg
      121 ccatgggtcc gggacgaaac agtgagacat tggccatgat atcggtatag cagacgggct
      181 gcagcttgag cagaaggttg cgcatgcctg gcgactcgag ttgaaacaca ccaagcgtgc
      241 gagcttcacg cagcaggcga taggtcggct ggtcgtccat ggggatggtc gacagactga
      301 ggtctgtgcc tgtttcggcg cgaaccatag cggttgcgtc cactgcggcg gacagggtgc
      361 gcaatccaag caggtccatc ttgaccagac ccaggtgctc gacatcgtcc ttgtcatact
      421 gtgtgatgag acagccagtg cctgacggtt caaggggaac caggtcggtg agaggaaagg
      481 gcgcgatgat caaccctgcc aggtgtgtag acagcgtgcg gggcagcccc atgatgcgtt
      541 cggccaggga tacccagcgt tcgatgcttc cataggggaa cgtggccagc tcgggcaatt
      601 tggcgatgac gtcgctgatc cggcggtgac tgtaccaggg caggagtcgt gtggcatcgt
      661 cgacctcctc cttgctgtac cccaatgcct tgcctacgtc gcgaaaagcc cctcggatgt
      721 tgtatgtgtt cacattggct acgcgacaga ctctgtcttc tttaaatacc agaaacagtt
      781 ctgagacgac ctcgtcccgg cgcaatgcgt caaaatcgat atccacatcg ggcaggctct
      841 tgcgtccctc attgagaaac cgttcaaaca gcaagttgca ctgtatggga tcgacctgtg
      901 tggcgtcgat gcagtagcag acgatcgaat tggctcccga cccgcggccc gagtaggcga
      961 cgtctttgcg ccgtgctatc tcgatgatgt cgcgtacgat gagaaagtac tcgtccatgt
     1021 gcatgtgccg gatgatgccc agttcgtgct cgatacgctc ctccacggca gtggtgacga
     1081 tgccgtactt ttcacgggcg ccggcataga cgatatcacg gagactgcgc gtcgccgtca
     1141 ggactgaact gggagtcttg aagccatcca gaggcagctc cacctggcat tctgccgcga
     1201 tgcgtccact ggcagacagg gcctcgggcc agtctgcgaa cagctcctgc atctcgtctg
     1261 ctcccctgaa ccagttctcg gcgttgacat gggatcgcct gctgcgggcg tcaacatctt
     1321 ccagtgaacc aatagccaga agcatctcgc gtacctctgc gtcgatgcgg tgcagatgag
     1381 tgacgccacc agtggcgcag gcgggcacgc cggtttcatc tgacaaacgg cgcatgcggt
     1441 acagcaggcg tgtggagtct gtaaacaggg ggttctggac gtccaggtac aggctggtct
     1501 cgaagagttt gcgcatctcc tccagcaggc agcgtgcctt gcatggccgg cgtgtcgaca
     1561 gagaccggta gagcaggctc tcgggactgc catacaaagc gatcaggtga ccggcatatg
     1621 gagccagctg ctcgagagtc agacccggat ggatccggcc ctgtgcctgg gcacgggtca
     1681 ccaggcgaca aaggttgcgg tatccctcca gatcgcgtgc caggacgacg acgtggtcgt
     1741 ctggctcgtc gctcagcaat gtcagctcag tgccaaaaac aggggtgact ccagctgaac
     1801 gcgctgcacg atcgaagcgg atggcgccgg ccagggtatt gctgtcggtg atggcacagg
     1861 aggagtagtc cagttcgtga ctgcgttgtg ccaggtcttc gatacggatg ggactctcat
     1921 gaaagctgaa gcccgtgtga aggtgcagat ggacaaagga catatcagac cactcatggt
     1981 gtctctgtca atccatcagc cggaccatgt accagtgtcg atggacgttg tctcttgcga
     2041 ccgtcgctat catcttgtcg tcgctcatga tcgtgtagta ggttgcatcc tccgtatggc
     2101 cggaagtaaa tcgtccactg gcccacatct tgaggacacg gtccacatgt ttgacctcgc
     2161 cacggaacac aaaggcggaa aggtggccgc ctcgttcctc gatgtagagc agtggcaggt
     2221 tatcggaagg ggtcggcaag accgggaagc aagatgtggg attcatacgc cctccttagg
     2281 acaggtgctc acggacaagg ccgacaacct tgccgataat gctcacgtcg ctggtgacag
     2341 gaatagggtg gtacgcaggg ttggccggca tgagaaagat cccgccatct ccctcgtgca
     2401 gcttcttgac ggtcgcttcg tctccaaaca gagccacaac gatgtcaccg ttgtctgcgg
     2461 tctgctgttt gtggacgacg acgatatccc ctggcacgat gtgttcgccg atcatggagt
     2521 cccccttgac cttgaggcca aagtagtcgg cagtcgcggg cgtcggcggg gtgacaaaga
     2581 tccatttctc gatgttctcc tcggccagga tgggggttcc ggctgcgaca gtgcccagca
     2641 ccggtatcat cagcgatggg gtggccggcg tgcccttgat gaggtccggc tgagggaggt
     2701 accctttgcg ttcgagcgtc ttgacgtata cgacgaccga gttgagagaa ttgaatccaa
     2761 agtgtcgccg tacatcctcc agactggggc gaaatcctgt ctggcgtgcg cgtacctcga
     2821 tgaattccag cactgcttgc tgcttgccgg tcaactcttc catcatccac ctctattgta
     2881 ctgtaggtac tacagttgta gtgtatacac tgtcaaagaa aaatgcaata ggaggcaggc
     2941 gaaaataaat gatcaggatg tgaaacgtat aaagcaatat cgggcacatg atgtgcccga
     3001 tattgccgtc gactgacggg atgtgtgcag agggctacag gcggcggccg tatctccctg
     3061 cgagagctgt cactccctga atggggtccg ctcggaattc atctgccact gccgaagcag
     3121 ttcgctgcag atgttcagcg cttccatgcc cagcccttct ccgtatgccg taagcagacc
     3181 ggcgagagct ttgcgagaag cgtctgccgg cacgttctga aggagcctgt ctgtgtccat
     3241 ggcctgtgct tccagtggca ggagagcagc ccggacttcg gcgatgtgtc cggcatagtc
     3301 gcagtcgacc gccgtcacaa acagtcggaa tgcccagaat gcggagctca tgtccaggtg
     3361 gtccgggtct cctgtggcgt aggggtctgg gtatccgaga cccgtttcca tcatggccag
     3421 cggataccat ggcgtgtaag gaagctcgtc ggggattccg gaggctcgcc agacagtcac
     3481 ggactgtggt ccattgttct ggttcctcag ttcggtcacg gaggattcct gagtggtcca
     3541 gaccgaaatc ggacgcatgg ttgttcgagc tacgttcttg tggaagctgc cactcattgc
     3601 gagtgcctgc cgggtggact gtccgtgagg tgtaacgcag cgcaggcacg ccatgacatc
     3661 gccaatggtc acagggtgct ctggcacgca tgaaaatggc atgtcgtcca atgggaactc
     3721 gtgcccggtg agcaggaaga gccctgtctg ctgacgactg gtgctcaatg aggagacatt
     3781 gatctcgggc ggcccgtacg cttccttgaa atcgaagggt tctgttccag tggagtcgta
     3841 ccacgcgcgt tgtatggcat gggcctcaag atctgtcgag gcaaggaacc agtctgtgtt
     3901 cgccaggtcc accttcctgg agatgaggta gtttggcagg acgacgaccg cgtcgtcggg
     3961 gacgcgttgt gccagccact cgtggccccc ggggatctga agaacccatg cttcgttctg
     4021 gtcggcaatc gtgatgatgc gggcgtcaca gtagccgtac gcttcgacaa gccggcctgc
     4081 gatcaggacg ccctcgcggg cggagtgagc ctgttcggcg accagttgtg gcaggaagtg
     4141 accgatgcca ccccgctcca gttcgggctg ctgttcgcgt gtacctgcag cgctgtcgca
     4201 gcagatgctg accccctcgc agttcaggaa gctgtctgcg aagtcttgtc cgaccagctc
     4261 tgtccagtcg taggccaagg tacctgccgg caccggaagc tccagagagg tatggggttc
     4321 gcggtacagc agtgtcccgt ctctgagtcg tgtggcggct ggatgttcgc ccgccttgac
     4381 gatgtggtga cgcacgacca gccgatgcgc gtcgtcctcg ttgtggccga cgacaagaga
     4441 acccgactgc gacgcggatc ggccgatgat gaccgtcgta cagtcgagct catcgttttc
     4501 gagtccaggt gaacgcatca gcgcagctcc agcgtttgca gagcggcagc gatgcgggtc
     4561 aggccttcct gtacagtcga gcgggggcat gcgatgttca tacgggcgaa gccctcaccg
     4621 ccaggtccaa agacgtatcc ctcgtccaga ccgactttgg ctttcttgcg caggaagtcc
     4681 gagagctggt ggatgtccat gccaagcgcg cgaaagtcca gccacatcag gtaggttccc
     4741 tcgatgggca tgaccttgat ctgtggcata tggtcctgga cgaacagtcg gacatacgtc
     4801 acattgcctt cgatgtagtg cagcagctga gtgagccagt cgtcgccctc gcggaatgaa
     4861 gcctcgagcg cagtgtatcc aaagggtgtg acactgccca cggctccgct gatgcgtcgt
     4921 tgcatcgctg cgcgcatccg gggattggga acgacgatga agctggtggc cagtcctgct
     4981 agattgaacg tctttgacgg tgccatgcac acgatgctgt tttgctcaag ttctggactg
     5041 agagaggcaa acgggatgtg acgcgaaccg ttcagcagca gttcgcagtg tatttc
//
LOCUS       DATMZD010000258         2177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_38637,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000258 DATMZD010000000
VERSION     DATMZD010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38637"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VN478_05355"
     CDS             <1..260
                     /locus_tag="VN478_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006066225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HWQ22133.1"
                     /translation="EMGTAMVSSLNMLGRFKGWFVPVGIHTLIVGVGSTSRKPGIVDG
                     RIEIREFLDLTVLVDHDVVDGAPAARALSRLTKIMESGFGL"
     gene            862..1881
                     /locus_tag="VN478_05360"
     CDS             862..1881
                     /locus_tag="VN478_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020491098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifH"
                     /protein_id="HWQ22134.1"
                     /translation="MSNWKAGLKADPTDWLLEEDNPSVRYFTLTDILDRAQDDAAVQT
                     ARREIMQRGIVPDILARQRQPAYLQTLPRFYTDKYEGLVWSLIVLAELGADVNPQISR
                     QCEYILANSQETQDGGFSMHTAKKTGGGRIAEVIPCLTGDMVWSLIRLGYLDDPRLRK
                     GIDWITRYMRFNDGVEDTPQVPPYDRLEMCWGKHACHMGVVKALKALSAMPKETRAAG
                     EIGDTIQKATEFLLIHHIYKRSHDLRRVSKPGWLRFGFPLMYQTDALEVLDILTELGV
                     EDRRMDEAIDVVLTKQDDMGRWRLENTCGSDGLLVPMGQKDEQSKWLTLRAVRVLKRY
                     RHRQA"
BASE COUNT          496 a          597 c          642 g          442 t
ORIGIN      
        1 gagagatggg gaccgcgatg gtgtcttcgc tgaatatgct gggcaggttc aagggctggt
       61 tcgttccggt gggcattcac acgctcatag tcggggtcgg ttccacgagt cgaaagccgg
      121 gcatagtgga cgggagaatc gagatacgag agttcctgga tctcaccgtt ctggttgacc
      181 acgacgttgt cgatggcgct cctgcggcgc gagctctgtc aagactgacg aagatcatgg
      241 agagtgggtt cggattgtag aagaatgcag gctctggaga attgagtcca gtcaactcat
      301 tccacgacac tcttcatcct ggtttgatgg caggttcaga cgaagaaccg ggacacatac
      361 cgtgccaacc gaaaggctga cctcgattcc tttgctgggg ttgctcctcg ggcatgatgg
      421 gcagtcgagg gcgacatttt accacgagca ctcgacaggg gcctgctccc ggtctttgcg
      481 cttgctgagt gtgccagcca tcgttccttg gccatgatga cgactgcacg gctcccttcg
      541 tcacgcattg agaaccttcc tgctcggtaa tgacactcct ggagacggtc aggcacagac
      601 agcccctctc tcttgacact cggtcttcac gtttgccgag tcagcggact gtcgtacctg
      661 caggtccttc attgacatct tgacgtttcg gcccttcgtg cccgtcgtgc tttgcggaag
      721 gctccgtgtg tgcagagccc gccggcacca caacacgagc acgaccttga caaacttatt
      781 ctcacttcct gctcaacgtg tagaataatg tgcgagagtg tgactttttt gcgccgtcgt
      841 catctcatcg gaggcacaga gatgagcaat tggaaagccg ggctcaaagc agacccaacg
      901 gactggctgc tcgaagagga caatccttcc gtgcgctact tcacgttaac ggacatcctg
      961 gacagagcac aagatgacgc ggcagtgcag actgccaggc gagagatcat gcagaggggc
     1021 atcgttcccg acattcttgc cagacagcga cagcccgcgt atctccaaac cctgcccaga
     1081 ttctataccg acaaatacga aggccttgtg tggtcgttga tcgtgctcgc ggaactgggc
     1141 gctgacgtca atccacagat aagcagacag tgcgagtaca tactcgccaa ctcgcaggag
     1201 acgcaggacg gcggtttctc gatgcatacg gccaagaaga cgggcggagg caggatagcg
     1261 gaagtcatcc catgcctgac tggagacatg gtgtggagcc tgatacgtct cggctatctc
     1321 gacgaccccc ggctccggaa aggtatcgac tggatcaccc gatacatgcg attcaatgac
     1381 ggcgttgagg atactccaca agtcccacca tatgaccggc tcgaaatgtg ctggggcaaa
     1441 catgcctgtc acatgggcgt ggtcaaggcg ctaaaggcac tgagcgccat gccgaaagag
     1501 actagagcgg ccggagaaat cggcgatacg atacaaaagg caaccgaatt cctgctgatt
     1561 caccacatct acaaacgcag ccacgacctg cggcgagtat ccaagcccgg atggctcagg
     1621 ttcggcttcc ctttgatgta tcagactgat gcactggaag tcctggatat cctcaccgaa
     1681 ctcggcgtgg aagaccgtcg catggatgag gccatcgacg tcgtcctgac aaagcaggac
     1741 gacatgggca gatggagact ggagaatacc tgcggcagcg acgggctgct tgtcccgatg
     1801 ggacagaagg atgaacagag caagtggctt acactcaggg cagtgagagt gctcaagaga
     1861 tacagacata gacaggcgtg agcacactga ctcagcctga agtcgccctt cttgctctgc
     1921 cctgcgctac gcatacgcca tagcgccgac tgtccctgtc ttctcgtcag cgtgatacac
     1981 gaagcagact ccatcacatt cgttcaaccg ttcatgccag cgacactgtg aattgacacg
     2041 gttctgcgct tatggtagat tgcagtcaga tttccagtcc tggacggagg taggaagcaa
     2101 tgggcagagg cggtggctcc ggcggcagaa gtagcggtgg ctccggcggc gggagcagga
     2161 gcagcggcgg tcgcagt
//
LOCUS       DATMZD010000259         4596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_98463,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000259 DATMZD010000000
VERSION     DATMZD010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4596
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98463"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="VN478_05365"
     CDS             <1..606
                     /locus_tag="VN478_05365"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HWQ22135.1"
                     /translation="VLENTMRLLHPFVPFVTETVTQQLYGGDHMIMTAAYPEADGMII
                     YDKDAMQADFLIGFIKAVRNLKAVFHLPPTEKQSVVAVGADSDITAITANAEAVKFVG
                     RFADISTQLDPAQRYIKGAYGTMQVMILAKSDFDYEGELKVLDAKLALLRADIAKIEA
                     RLGNQDFLARAPAEVVDKDTQKLEDLRAEEQLIGTFLRGQG"
     gene            619..1971
                     /locus_tag="VN478_05370"
     CDS             619..1971
                     /locus_tag="VN478_05370"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HWQ22136.1"
                     /translation="MEIRERSRTYAEALDCVFSLSKFGSKLGLERIRRIMSSLGNPER
                     QLRFVHVAGTKGKGSVCAFAARMLEESGYTVGLYISPSLVDVGERISVNGAMLSPKEF
                     LEAFDVVWGAIGSVSADDPVTVFEVFTAMAIVHFVRRKVDVVVWEVGLGGLYDATNVI
                     PLPEVAVITNIGLEHTDRLGDTVEEIATQKAGIIKQEGVTVLGHQDYPAAQTTLLAQA
                     EKMGNQILLAGRDYSFSSQRFTPDSVSFDYEGVVLGRRVSLPDVTISLRGIHQVQNAA
                     SAITAVVALAAKDGIDIPEPAMRRALKQTFWPGRMELVPGTPTILLDGAHTRTAASYL
                     VESLHAHFPGQRFTMLFGVLMDKDMDGIAAILASEVDTIVLTRVPDNGRAASPWELEE
                     KFLHYHPHGSIVVEDDSSRAFEKARDLTPDGSWLLVTGSLYLVGAVRKLMGLYTFVRP
                     "
     gene            2230..2484
                     /locus_tag="VN478_05375"
     CDS             2230..2484
                     /locus_tag="VN478_05375"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HWQ22137.1"
                     /translation="MADKLNNDVLKLERSYYAFLMDEQDRKCPVCGHEVKARYRFCPA
                     CGTELRTVCPACGELMETNWKSCPHCGHRIEQTEGKGEMV"
     gene            2485..3825
                     /gene="rsxC"
                     /locus_tag="VN478_05380"
     CDS             2485..3825
                     /gene="rsxC"
                     /locus_tag="VN478_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HWQ22138.1"
                     /translation="MSLKTFRGGIHPSEHKEPTRDLPIERVPAPASVVIHLTQAVGAQ
                     NQPLVKVGDRVLKGQKIGDVQACPAAAVHASITGIVKKIEPAPQSNLKEGMAVFIEAD
                     GTDETAYMAPLNAFACSREDAIQRLRDAGIVGMGGASFPTDVKLAPPADKPIDTVIAN
                     GAECEPYLTIDYRTMIESPADVVEGLAIAAHIVGARKAFVAIESNKADAVRVIEQEFA
                     GHSHGIPMEAVVVATKYPEGGEKMLIKAVAGREVPSKGLPFDIGVICQNIGTLKAIKE
                     AFSDGKPLIERGFTVSGGACTTPKNLYAPIGTVLADLVPGVIQIGDDVRKVIFGGPMM
                     GVTVSSLQTPIAKGTSGVLFFTAAESRQYAERQCIRCGRCANACAARLMPMLLHAAIS
                     SGDLDRADAIGLVDCFECGACSYVCPARIPLTQQFKLGKDLLRNQRAKEARHGK"
     gene            3815..>4596
                     /locus_tag="VN478_05385"
     CDS             3815..>4596
                     /locus_tag="VN478_05385"
                     /inference="COORDINATES: protein motif:HMM:NF015100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HWQ22139.1"
                     /translation="MENRATRFVMSGAPHSLTAFDIQKTMGMVIIALLPALAGGVYFF
                     GPRALLVTAVSIASAVAAEFLFRKALHRETHVTDLSAVVTGVLLALIVPVSSPLWMAA
                     VGSAFAIVVVKELFGGLGGNFANPALVGRAILLMSWPAFMTRWSPSLHAAAVDATTTA
                     TPLGILKLGGALTDVMESLGVTSKPALYLQMFLGNRSGSIGETSALLLLIGAVFLIVT
                     RIIDWRAPIAMIATTFVASWLFGRDPLFSILAGGILLGAFFMA"
BASE COUNT          933 a         1391 c         1352 g          920 t
ORIGIN      
        1 gtgctggaga acacgatgcg cctcctccac ccgtttgtcc cgttcgtcac cgagactgtc
       61 acgcagcagc tgtacggcgg cgaccatatg atcatgacgg cagcgtaccc ggaagccgac
      121 ggtatgatca tatacgacaa ggacgccatg caggccgact tcctcatcgg cttcatcaag
      181 gccgtccgaa acctcaaggc agtcttccac ttgcctccaa ccgagaagca gagcgtggta
      241 gcggtcggcg cggacagcga catcaccgca atcacggcaa atgccgaggc agtcaagttc
      301 gtcgggcggt tcgccgacat cagcactcag ctcgacccgg cacagcgcta tatcaagggc
      361 gcatacggga caatgcaggt catgattctt gccaagtctg acttcgacta tgaaggtgaa
      421 ctcaaggtcc tcgatgccaa actcgccctc ctgcgcgcgg acatcgccaa gatcgaggca
      481 cgtctgggca accaggactt ccttgcgcgt gctccggcag aagttgtcga caaggacacg
      541 cagaaactgg aagacctcag ggcagaagaa caacttatcg ggactttcct gagaggacag
      601 ggctagctct ggcatcacgt ggagattcgc gagcgaagca ggacgtacgc tgaagcactc
      661 gattgtgtct tctcactctc caagttcggt tcgaagctgg gcctggagag aattcgccgt
      721 atcatgtcca gcctgggaaa ccccgaacgg cagctccgat tcgttcacgt cgcaggcacc
      781 aagggcaagg ggtccgtctg tgctttcgcg gcccgcatgc tcgaggaatc cggctacacc
      841 gtcggtctct acatctcacc ttctctcgtg gatgtgggcg agcgcatttc cgtcaatggc
      901 gcaatgctct caccgaagga attccttgag gcttttgacg ttgtctgggg cgcgatcggc
      961 agtgtctcgg cagatgatcc tgtcacggtc ttcgaggtct tcacggcgat ggcgatcgtc
     1021 catttcgtcc gcagaaaggt cgacgtcgtc gtgtgggaag tcggccttgg agggctgtac
     1081 gatgccacca acgtcatccc cttgcctgag gttgccgtca tcaccaacat cggactggaa
     1141 cacacggacc ggcttggaga cacggtagag gagatcgcga ctcagaaagc gggcatcatc
     1201 aagcaagaag gcgtaacggt cctcggtcat caggactacc cggcagctca gacgactcta
     1261 ctcgctcagg ccgagaagat ggggaaccag atactcctcg ctggacgaga ctacagcttc
     1321 tcctcgcagc ggttcacgcc ggactccgtc tcgttcgact acgaaggcgt cgtgttgggg
     1381 cgacgggtct cgcttcccga cgtgaccata tctttgcgcg gcatacatca ggttcagaat
     1441 gcagcgagcg ccatcacggc cgtcgtcgcc ctggctgcca aggatggcat cgacatcccc
     1501 gaaccagcca tgcgacgcgc tctgaaacag acgttctggc ctgggcgcat ggaactcgtt
     1561 ccgggcacgc ccacaattct tctggatggc gcacacacgc gaacggcagc cagctacctg
     1621 gtggaatcgc tgcatgctca cttccctggc cagcgcttca cgatgctgtt cggcgtgctc
     1681 atggacaagg atatggacgg catcgctgcc attctggcat cagaggtcga tacgatcgtt
     1741 ctgacgcgcg tccccgacaa cggccgcgct gccagtccgt gggaactgga ggagaagttc
     1801 ctgcactacc atcctcatgg ctccatcgtc gtcgaagacg acagctcgcg cgcattcgaa
     1861 aaggctcgag acctgacgcc cgacggctcc tggcttctcg tgacaggctc cctgtacctt
     1921 gtaggagcgg tccgaaaact gatggggctt tacacctttg tccgtccgtg atactctttt
     1981 gaatttggag gtggccacat tactacacta ctggtagagt tctggcagaa cacggttgtc
     2041 ccaaatctca gttccatcct gctgggaacg gccgtcctcg tcgctgtcat cctcgtatgg
     2101 atcatcaagg acacgtcaag gagaggtgcg aacaccgtcc tgtggtcggt cttcacagtc
     2161 gtcggtctcg gccttctgcc gctgatcgtg tacttcctgg ttcgcgaccc actgacactg
     2221 gacgaccata tggccgacaa gctgaacaat gacgttctca agctggaaag aagctactac
     2281 gcgtttctca tggacgagca ggaccgaaag tgcccggtgt gtgggcacga agtgaaggcg
     2341 aggtaccggt tctgtcccgc atgtggtact gagcttcgca cggtatgtcc agcgtgtgga
     2401 gagctcatgg aaacaaactg gaagtcgtgc cctcactgcg ggcacaggat cgaacagacg
     2461 gaagggaaag gggagatggt ctagatgagt ctgaaaacat tcaggggcgg catccacccc
     2521 tctgagcaca aggaaccgac aagagacctg cctatcgaac gcgtaccggc gccagcgtct
     2581 gtcgtcatcc acctgacgca ggccgtcggc gctcagaacc agccgctcgt caaggtcggg
     2641 gaccgcgtcc tgaaaggcca gaagatcgga gacgtccagg cctgccctgc ggccgcggtc
     2701 catgcgtcga tcaccggcat cgtcaagaag atcgagcctg caccccagag caatctgaag
     2761 gaaggcatgg ccgtcttcat cgaagcagat ggcactgatg agaccgccta catggcgcct
     2821 ctcaatgcct ttgcctgctc acgcgaggac gcgatccagc gtttgcgcga cgccggcatc
     2881 gttggcatgg gcggtgcgtc cttccccacc gacgtcaagc tggcacctcc agcggacaag
     2941 cccatagaca cagtcatcgc caacggggcg gagtgcgagc catatctgac aatcgactac
     3001 cggacaatga tcgagtcacc tgctgacgtc gtagagggac tggccatcgc ggcgcatatc
     3061 gtgggagcca ggaaggcatt cgtcgccatc gaatccaaca aggcggatgc agtgcgcgtc
     3121 atcgagcagg agtttgccgg gcacagccac ggcattccga tggaagctgt tgtcgtggca
     3181 acaaagtatc ccgagggcgg agagaagatg ctcatcaaag cggtcgcggg ccgcgaagtc
     3241 ccctcgaagg gtctcccgtt cgacatcggc gttatctgcc agaatatcgg gaccctcaag
     3301 gccatcaagg aagcattcag cgacgggaaa cctctcatcg agcgtggatt caccgtctcg
     3361 ggcggagcat gtacgacacc caagaacctc tatgcgccaa tcgggacagt actcgccgac
     3421 cttgttccag gtgtgatcca gatcggcgac gacgtgcgca aggtgatctt cggcggcccc
     3481 atgatgggcg tgactgtgtc atccctgcag accccgatcg ccaaaggcac cagcggcgtc
     3541 ctgttcttca ctgctgccga gtcaagacag tacgcggaac gtcagtgcat tcgttgtggc
     3601 cggtgtgcca atgcctgcgc agcccggctg atgcccatgc tcctgcatgc tgcgatctcg
     3661 tcaggcgacc ttgaccgtgc agatgccatc ggtctcgttg attgcttcga atgtggtgcc
     3721 tgcagctatg tctgccctgc acgcattcct ctcacccagc agttcaagct cggcaaggat
     3781 ctgttgcgca accagcgtgc caaggaggcc cgccatggaa aatagagcca caagattcgt
     3841 gatgtctggc gcaccgcact ctctgaccgc cttcgacatc cagaaaacga tgggaatggt
     3901 catcattgcc ctgctgccgg ccctcgcagg tggcgtatac ttcttcggac cgagggcact
     3961 gctggtcacc gccgtatcta ttgcgagcgc cgtcgctgcc gaattcctgt ttcgtaaggc
     4021 tctccatcgc gagacacatg tcacagacct gtcggccgtt gtgacggggg tgttgctggc
     4081 actcatcgtg ccagtgtcct caccgttgtg gatggcagcg gttggctccg cattcgccat
     4141 cgtggttgtc aaagagctgt ttggagggct gggtggcaac tttgcgaacc ctgcgctggt
     4201 cgggcgagca atcctgctga tgagctggcc ggccttcatg acgcgctggt ctccatcgtt
     4261 gcacgcggcg gccgtcgatg cgacgactac tgcgacgcct ctgggtatcc tcaagctggg
     4321 cggagcactc accgacgtca tggagagtct cggggtcacc agcaagccag ccctgtatct
     4381 tcagatgttc ctcggcaacc ggtcgggaag catcggggag acgtctgcac tgctgctcct
     4441 cattggcgcg gtgttcctca tcgtgacacg catcatcgac tggcgagctc cgatcgccat
     4501 gattgcaacg acgttcgtcg cgtcctggct gttcgggcga gacccgctct tctcgatcct
     4561 tgccggaggc attctgctgg gggccttctt catggc
//
LOCUS       DATMZD010000260         4873 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_127860,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000260 DATMZD010000000
VERSION     DATMZD010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4873
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127860"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="VN478_05390"
     CDS             complement(<1..208)
                     /locus_tag="VN478_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22140.1"
                     /translation="MPEKINDPVTSNLPVDNGGAATGARSQESLFRTRALQSAIFNSA
                     NFSSIATDAKGVIQIFNVGAERMLG"
     gene            complement(383..826)
                     /locus_tag="VN478_05395"
     CDS             complement(383..826)
                     /locus_tag="VN478_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015159581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWQ22141.1"
                     /translation="MSEKIILLVEDNPDDAALTLRALSKNNVANQVVVAGDGEEALDY
                     LTSHGKYAGRAPTNLPELVLLDLKLPRVSGLEVLRAIRADPRTRMLPVVVLTSSTDEP
                     DVVTSYQLGANSYIRKPVDFDQFLEAVRQLGLYWLVLNQAPPVDH"
     gene            complement(1045..3858)
                     /locus_tag="VN478_05400"
     CDS             complement(1045..3858)
                     /locus_tag="VN478_05400"
                     /inference="COORDINATES: protein motif:HMM:NF019314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWQ22142.1"
                     /translation="MTRESFVALVGNATLLLSLVYVYDLLNVLRRLSGTWIRQVIAGL
                     AIGAIGFFVMLMPWTFVTGVTFDTRSVLLAVSGLFFGAIPTAIAALVMAAVRLIQGGG
                     GAWTGVSVIAASAAIGVVWRRAQRRPLAALSFGSLFMLGLVVHVVMLVLMFTLPVQTA
                     IQIHKSIAIPVLVIYPISTAAYGTLMVDRLRRDEAAAAVRESESRFRALFEQAAFGVA
                     KADVRTGRFALVNQHFADLLGYTPDELQGLNWQAITFADDRAAQHERTQELVSGRIRS
                     FVVVKRYVRRDGSIMWASVTVSRLWAEGDKPDFTMAEIEDITERRAMERKLEQLASFP
                     GQNPNPVVEVAVDGTVRYANPAALEALERLGLQKDARQFLPESADELGQLRVLCEQHP
                     QARELALGKATFYRTVMAPPEPGTLRVYAIDITDTKTAQSRQERYLSMLDRSLNEIYV
                     FDEMSLRFVYVNGSALHNLGYSLGELQQMTPIDIKPEFTSPDFDELLLPLRDGTRQLL
                     VFETVHRRKDGSTYPVEVHLQRVQGEGQSWFLAFINDVSERQHMQAQLLQSQKMEAVG
                     ELAGGVAHDFNNMLTGILGNVAIIHDTMSPRDPLRENVHAIETAAQEAANLTRGLLTF
                     SRGAVVQPVALDTNASVERIMAVLKQSLPESLTIVSNLESDTWNVYMDQAQMTQILLN
                     LGVNARDAMNGRGTITVATRNAVVDASYVETHHFARQGDFVVVSVADTGSGIPPEALP
                     HIFEPFFTTKPVGSGTGLGLSVVYGAANQAGGWITVQSEAGRGTLFEVYLPRSTEQQP
                     AEAPATAADMLPCIGTVLVVEDEPVVSAVTRSLLTRAGCDVLTAVDGASALHALQEHA
                     DSITLVLLDMTMPGLTTEEIVSELRRLYPTLPVLLTSGFAPSDTVTQLVDTGSVQGFL
                     PKPYDFHELLQRVGQLTHAKGDS"
     gene            4544..>4873
                     /locus_tag="VN478_05405"
     CDS             4544..>4873
                     /locus_tag="VN478_05405"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWQ22143.1"
                     /translation="MDEQAQFLQWLMEQMSLSQVELAQRLGISASLVSRLVGGERRLT
                     LSMLRHIANVFDLPQPEVFRRAGVLQEPVLVEEAAEQYAAEAAPESEAEAAALRAQLE
                     DLRDPRKA"
BASE COUNT          930 a         1445 c         1559 g          939 t
ORIGIN      
        1 atccaagcat gcgctcggcc cccacgttga agatctggat gacacccttc gcgtccgtgg
       61 caatgctcga gaagttggcg ctgttgaaga tggcgctctg cagggctctg gtcctgaaaa
      121 gggactcctg actgcgtgct ccagtcgctg cccctccgtt gtccacgggc aggttcgatg
      181 tgacgggatc gttgatcttc tccggcatga ccgacctctt ctcaggtgac gacaaacgat
      241 aatgtgcgcc cagttgcaga cgtactgacg acgtaacaga tatacttagt atgtgcaaat
      301 gtcaagaaag gacaactgtg ggttatcgtg actactctga gggggctggc agcgtagaac
      361 gggcatcatg ggtttgcgca cgtcagtgat cgacgggagg tgcctggttc agcaccagcc
      421 agtacaggcc gagttgccgt acagcttcca ggaactggtc gaagtccaca ggtttgcgga
      481 tgtagctgtt ggcccccagc tggtagctcg taaccacgtc tggttcgtcc gtggatgaag
      541 tcaggactac cacagggagc atgcgtgtcc gcgggtccgc ccggatagct cgcaggactt
      601 cgaggccgct cactcgtggc agcttgaggt ccagcaggac gagttccggc aggttcgtcg
      661 gcgcgcgtcc tgcatacttg ccatgcgagg tcaggtaatc cagcgcctct tcgccgtcac
      721 cggcaacgac tacctggttg gcgacgttgt tcttgctgag cgctcgcagc gtcagtgccg
      781 cgtcgtcggg gttgtcttcg accagtaaga tgatcttctc actcatggtt ggtcctcctt
      841 ggttgcagcc gatgtatgca gctctggaac tgtgacgtgg aacatcgctt cccggttgac
      901 tgcgccatcg gcccacacgc cgccacgccc gactgtcctc tggatcgccg ccggctcgac
      961 catcacccgg ttagcgccag acggagacca cggcggcaac cagcagcgag acgctgctga
     1021 cgagcgctat gaacgagccc ctgctcatga gtcacctttc gcgtgagtga gctgcccgac
     1081 acgttgcagt agctcgtgga agtcatacgg cttgggcagg aatccctgca cgctccctgt
     1141 gtccaccaac tgcgtgaccg tgtcgctggg cgcgaacccc gaagtaagca acacgggcag
     1201 agtggggtac aggcggcgca gctccgacac gatctcctcc gtggtcaagc ccggcatggt
     1261 catgtccaga agaacaagag tgatgctgtc tgcgtgctcc tggagggcat gcaacgcgga
     1321 ggcgccgtcg acggctgtca ggacgtcgca gcctgctcgc gtcagcaacg accgggtcac
     1381 tgcgctgacg accggctcgt cctcgacgac gagcaccgtg ccgatgcagg gaagcatgtc
     1441 cgccgcagtg gcgggggcct cagcaggctg ctgttcagtc gaccgtggga gatagacctc
     1501 gaacagggtt cccctgccgg cttcagactg gactgtgatc catccgccag cctggttggc
     1561 ggcaccgtag acgacggaca ggccgaggcc cgtgccgctc cctacaggct tcgtggtgaa
     1621 gaagggttcg aagatgtgcg gcagggcttc cggcgggatg cccgatccgg tgtcggcgac
     1681 gcttaccacg acgaagtcgc cctgccgggc aaagtggtgc gtctcgacat atgaagcgtc
     1741 gacgacagcg tttcgtgtgg ctacggtgat ggtgcctcgg ccattcatgg cgtcacgggc
     1801 gttgacgccc aggttcagca agatctgcgt catctgggcc tggtccatgt agacgttcca
     1861 ggtgtctgat tccaggttgc tgacaatggt gagggattcg ggcagggact gcttcaggac
     1921 ggccatgatc cgctccacgg aggcgttggt gtccagagcg acgggctgca ccacagctcc
     1981 ccggctgaag gtcagcagtc cccgcgtcag attggccgct tcctgggcgg cagtttcgat
     2041 ggcatggaca ttttcacgga gaggatctcg tggggacatc gtgtcatgga tgatggcgac
     2101 attgcccagg atccccgtca gcatgttgtt gaaatcatgg gcgacgccac cggcaagctc
     2161 gccgacggct tccatcttct gagactgcag caactgggcc tgcatgtgct ggcgctcgga
     2221 gacgtcattg atgaaggcca ggaaccatga ctggccttcc ccctggactc gctgaaggtg
     2281 gacttcgact gggtacgttg acccgtcctt gcgccggtgt accgtctcga agaccagcag
     2341 ttgtctggta ccgtcccgaa gaggcagcag cagctcatcg aaatcggggc tggtgaactc
     2401 cggcttgatg tcgatcggtg tcatctgctg aagttccccc agggagtagc ccaggttgtg
     2461 gagcgctgat ccgttgacgt atacgaatct cagcgacatt tcgtcgaaga cgtagatctc
     2521 gttgaggcta cgatcgagca tgctgaggta ccgctcctgc cgcgactggg ctgtcttcgt
     2581 gtccgtgatg tcgatggcgt agacacgcag ggtccctggt tcgggcggcg ccatgactgt
     2641 cctgtagaat gtcgcctttc ccagggcaag ctcgcgggcc tgaggatgct gctcgcacag
     2701 gaccctgagc tgcccgagtt catcggcact ctcgggcagg aactgacggg cgtccttctg
     2761 caggccgagc cgttcgagtg cctccagagc ggcggggttg gcatagcgca cggttccgtc
     2821 cacggcaacc tccacgaccg ggttggggtt ctgccccggg aaggatgcca gctgctctag
     2881 cttgcgttcc atagctctgc gttcggtgat gtcctcgatc tcggccatcg tgaagtcggg
     2941 tttgtctccc tcggcccaca gacgcgatac ggtgacgctc gcccacatga tgctgccgtc
     3001 cctgcgcaca tagcgcttca ccacgacgaa tgagcgtatg cggccggata ccagctcctg
     3061 cgtgcgctca tgctgcgctg cacggtcgtc agcaaaagtg atggcctgcc agttcaaccc
     3121 ctgcagttca tccggcgtgt agccgagcag gtcggcgaag tgctggttga cgagagcaaa
     3181 tcggcccgtt cgcacgtctg ccttggcgac cccgaaggcc gcctgctcga acagggcccg
     3241 gaacctgctt tcgctctcgc gcactgcggc ggctgcctca tcgcgccgta gacgatcgac
     3301 catgagggtg ccatacgccg cggtcgagat agggtagatc accaggacag ggatggcgat
     3361 actcttgtgg atctggatcg cggtctgcac tggcagagtg aacatgagga cgagcatgac
     3421 gacatggacg accagcccga gcatgaagag cgacccgaaa ctcagcgccg caagcgggcg
     3481 ccgctgcgcg cgccgccaca ccacaccgat ggctgccgag gcagcgatga ctgatacgcc
     3541 ggtccaagcc ccacctccac cctggatcag ccggaccgcg gccatcacaa gagcggcgat
     3601 cgcagttgga atggcgccga agaatagccc cgagacggcc agcaggacag acctcgtgtc
     3661 gaaggtgact cccgtcacga atgtccaggg catcagcatg acgaagaagc caatggcgcc
     3721 gatggcaagg ccggcaatca cctggcgtat ccaggtccct gacagccggc ggaggacgtt
     3781 cagcaggtca tagacataga cgagcgatag cagcagggta gcgttgccga cgagggcgac
     3841 gaaggattcg cgtgtcacca gggagctccc atacacattc caggagcacg accaggaacg
     3901 actagcggtt cgcgtaccgt atcgtgccgc attgcgattc tccccatctg cggatcgccg
     3961 tcatgccggc tgtctggcgg catgcagccc gcttttctgc agtataggag gaccccgggc
     4021 ccagtcaagc gcgccgcctt gctgcaaaca gtgttccccc ggcgggggta cgccggggga
     4081 aaaaggaagg ggttctgcca gtcgttcccc ccaagccctt ttgtcaaagg cgaggggtgc
     4141 ttcccgcgaa ggtgggaatc cactcttgcc gccagatcac ttcatcctcg cggtgcagcg
     4201 gtcgtcacat ggcacagttc tcgtggtaac tggtgtgatg ccggcgcctg gtgtcgtgaa
     4261 aaacggtaca gaacacgcag cggaaccagc cagcatcagt aagaccgaga tcgacaggat
     4321 gaccttcttc atggtgtcgt caccctgcgc atgggtcgtc ccaaccttca gacgactcct
     4381 gacacccatg gtactcggga tttcctgtct gtgtaggtcg aagtgtgaag attgtacgag
     4441 ggccacaacg tcagccataa acagatgtgt gaaggagacg aatgacagaa tggcaattat
     4501 gtggtatact aacgatgcca agttggcaac aaggagatgc actatggacg aacaggcaca
     4561 gtttctgcaa tggctgatgg aacagatgtc gctctctcag gtcgagctgg cacagcggtt
     4621 gggcatttcg gccagcctgg tctcacggct ggtagggggt gagcggcggt tgacgttgag
     4681 catgcttcgg catattgcca acgtcttcga cctgccgcag ccggaggtgt tcaggcgtgc
     4741 cggcgtcctg caggagccgg tcctcgtgga agaggcggca gaacagtacg cagccgaggc
     4801 agcgccggag agcgaggctg aagcggcggc tctgcgtgct cagctggagg acctgcgcga
     4861 cccgcgcaag gcc
//
LOCUS       DATMZD010000261         3490 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_58059,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000261 DATMZD010000000
VERSION     DATMZD010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58059"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1274
                     /locus_tag="VN478_05410"
     CDS             <1..1274
                     /locus_tag="VN478_05410"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWQ22144.1"
                     /translation="LLVVGALCLLLAVVLARMAARPVADLADALERRSLQSLAGLAMR
                     ADVTELGRAQRASYALLQESEQLLEREQSQSSALSAVLDAVPQAIAILDGTDALLSTN
                     TQFDRLFSGNAVPAVGRHIAEVITFPDFLAAIERCISERQPQTVVAADRGRYYSCSVR
                     ELDGEAFHARRMLLVVEDVTDAMALPRIKADFVTNASHELKTPLTAIRGYLELLRDEP
                     DNARYLEVIERNVDRLIDLSSDISLLSRLESKAPEIELMDLHDLQQDLAELFEKQERD
                     TGVALRFSVDSEGRFLYADRLMILQMFINLIENAYRFTQVGAITVSATADASHVILSV
                     SDTGQGISANDLPRIFERFYSHSSDHGRPGTGLGLAIVKRIVLAHNGTIDVESTVHTG
                     TNFIIHIPRNLGPRRPAATSAEPSELQREDP"
     gene            1271..2329
                     /gene="pstS"
                     /locus_tag="VN478_05415"
     CDS             1271..2329
                     /gene="pstS"
                     /locus_tag="VN478_05415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HWQ22145.1"
                     /translation="MRRMNRTVVFVLACSLLLGTVGCRTAPGAPVKDLAIAGSTFAEP
                     LYVKQLDAWFQKTEVKAVYGALSSSGALRALQDRTIDVGASDLLMGVEDQARLPASIL
                     QIPTCLGAVAICVNLPGSPALRMDAALVSAVFRGDVKTWNDARIAALNPMLKLPETPV
                     AVIHRADGSGTTGIFTTYLAAADQTWREKVGAGQLVAWPAGIGAKGSAGMAAMVRQTT
                     GAIGYVEYTYAVQSGLTCVALRNRSGAFVLPSVASMQAAVPADLTADLQGTLLNRPGA
                     TAYPVVSLTWLVCYRDQSYDGRSRERAIAVYDLLSWLLGDGQTLAEPLQYGSLPDSVR
                     KAARAILAQLTWQGKPVG"
     gene            2462..>3490
                     /gene="pstS"
                     /locus_tag="VN478_05420"
     CDS             2462..>3490
                     /gene="pstS"
                     /locus_tag="VN478_05420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HWQ22146.1"
                     /translation="MSTTGRRILSIALVALIAAGLVVPGALSASAASTRTIVLTINST
                     TATVEGSNTSLDAAPFVASGRTMLPIRFVAETMGAGVSYKTKANGSVSQVDLGLPTGP
                     ATLKAVTLSLDSTTATVDGQAVTLDTTPVIRQGRTFLPIRFIAETIGAKVTYTTDPST
                     GRTKTVTIVVPIININVSTQMSGAGATFPLPLYTKMFDVYNSSLGTRVNYQGIGSGGG
                     IKALTDKTVDFGASDAFLSVAETASMGAPILHIPTCVGAVVLTYNLDGNPTLRFDART
                     VSDIFLGTITRWNDSRIAALNPGITLPDQAITTVHRSDGSGTTSIFTNYLSMAVPTWK
                     SEVGAGKSV"
BASE COUNT          679 a         1168 c          992 g          651 t
ORIGIN      
        1 tgcttcttgt cgtcggcgcg ctgtgcctcc tgctggccgt cgtgctggcg aggatggcgg
       61 cgaggcccgt agccgacctg gccgatgcgc tggaacgacg ctcactccag agtctggcag
      121 gtctcgcgat gcgcgctgac gtgacggagc ttgggcgtgc acagagggca agctacgcgc
      181 tgcttcagga aagcgagcaa ctccttgagc gcgagcagtc acagtcgtct gccctctctg
      241 cagtgctgga tgccgttcct caggctatcg ccatcctcga cggcaccgac gcgctgctct
      301 cgacgaacac gcaattcgac cggctcttca gtggaaacgc cgttccagcc gtagggcgtc
      361 acatcgccga ggtcatcaca ttccccgact ttctggcagc tatcgagcgc tgtatcagcg
      421 aacgtcagcc gcagacggtc gtcgccgcgg accgcggccg atactactcc tgcagtgtcc
      481 gtgaactgga cggggaagcg ttccacgcac ggcggatgct gttggtcgtc gaggatgtca
      541 cagacgccat ggcattaccg cgcatcaagg ccgatttcgt caccaacgct tcgcatgagc
      601 tcaagacacc tctcacggcc atccggggat acctcgaact gctgcgcgac gaacctgaca
      661 atgcccgata tctcgaggtc atcgagcgaa acgtcgaccg tctcatcgat ctctcctccg
      721 acatttcgct gctctcccgc ctcgagagca aggcgccgga aatcgaactc atggacctcc
      781 atgatctcca gcaggacctt gcagagctgt tcgagaaaca ggagcgcgat accggcgtcg
      841 ccctgcggtt ctctgtcgac agtgagggac ggttcctcta tgccgaccgc ctgatgatcc
      901 tgcagatgtt catcaacctt atcgaaaacg cctatcgctt cacgcaggtc ggcgcgatta
      961 cggtgtccgc tacggcagac gcatcccacg tcatcctgag cgtttccgac actggccagg
     1021 ggatctctgc caacgatctg ccccgcatct ttgagcgatt ctactcccat tccagcgatc
     1081 acgggcgtcc cggtaccggt ctgggcctcg ccatcgtgaa gcgcatcgtg cttgcccaca
     1141 atggaacgat agacgtcgag agcactgtcc acacggggac gaacttcatc atccacatcc
     1201 ccaggaacct cgggcctcgc agacctgctg cgacgagtgc agaaccatct gaactacaga
     1261 gggaggatcc atgagacgca tgaatcgcac tgtcgtgttc gtactcgcct gcagtctgct
     1321 tctcgggaca gtcggctgca ggactgctcc aggtgctccg gtcaaggacc tcgccatcgc
     1381 cggatccaca tttgccgaac cactgtacgt gaagcagctc gacgcgtggt tccagaagac
     1441 agaggtgaaa gccgtctacg gagccctgag ttcgtccggg gccttgcgcg ccttgcagga
     1501 ccgcacaatc gacgtggggg cttcggatct cctcatgggg gtcgaggacc aggcccgtct
     1561 gcctgcctca atcctccaga tcccgacctg tctcggcgcc gtggcgatct gcgtcaacct
     1621 gcccggcagt ccagccttgc gcatggacgc cgctcttgtc tccgccgtat tccggggtga
     1681 cgtcaagaca tggaacgatg cacgcatcgc agcgctcaac ccgatgctga agctgccgga
     1741 aacgccagtc gccgtcattc accgcgcgga tggatcgggg acgaccggca tcttcacgac
     1801 ctacctggca gccgccgatc aaacgtggag agagaaggtc ggggctggtc agctggtcgc
     1861 ctggccggcc ggcatcggcg caaagggcag cgcgggcatg gctgccatgg tgcggcagac
     1921 caccggcgcc atcggctacg tggaatacac gtatgctgtg caatccggcc tcacctgtgt
     1981 tgccctgcgc aaccgatcgg gtgcgttcgt cctgccgagt gtcgcatcaa tgcaggcagc
     2041 tgtcccggca gacctgacgg ccgacctcca gggaacgctc ctgaaccgcc ctggggcaac
     2101 cgcctatcct gtcgtcagcc tgacctggct tgtctgctac cgcgaccagt catacgacgg
     2161 ccgctcacgg gaacgggcta tcgcagtcta cgacctgctc tcatggctcc tcggcgacgg
     2221 acagaccctc gccgagccac tgcagtacgg cagtctgccc gacagcgtcc gcaaggccgc
     2281 cagggccatc ctcgcccagc tcacgtggca ggggaagccg gtcggatagc ggacagagag
     2341 gctgtgacga tccccgggcg gcttagcaga atctgaacag aacccgaacc ccggccaaac
     2401 atacctttct agtatgatga tcggaaaccc cccggggttt atcgatttgt cggaggtatg
     2461 tatgtcaaca actggaagaa ggatcctgtc gatagcgctt gtcgcactga tcgccgccgg
     2521 ccttgtggtc cctggagccc tttcggcatc cgccgcatca accaggacca tcgtcctcac
     2581 catcaactcc accactgcaa ccgtggaagg ctcaaacacc agccttgacg ccgcaccctt
     2641 tgtggccagt ggccgtacga tgctccccat ccggtttgtt gccgagacga tgggggcagg
     2701 cgtgtcctac aagacgaagg caaacgggag cgtctcgcag gtcgaccttg gactgccgac
     2761 cggccctgca actctcaagg ccgtcactct ctcgctcgat tccacaactg caaccgtcga
     2821 cggtcaggcg gtaacgcttg acacgactcc cgtcattcgg cagggaagga ccttcctccc
     2881 catccggttc atcgccgaaa cgataggcgc caaggtcacg tatacgacgg atccatcaac
     2941 cggcagaaca aagaccgtga cgatcgttgt gccaatcatc aatatcaacg tttctaccca
     3001 gatgtccggt gctggagcca ccttcccgct tccactgtac acgaagatgt ttgacgtgta
     3061 caacagttcc ttgggcacgc gcgtcaacta ccagggcatc ggctccggcg gcggtatcaa
     3121 ggcgctgacc gacaagaccg tggacttcgg ggcttccgac gcgtttctca gcgtggccga
     3181 gactgcaagc atgggcgccc cgatcctgca catccccacc tgcgtaggcg ctgtcgtcct
     3241 cacctacaac ctggatggca acccaacgct tcgctttgat gccaggacag tcagcgacat
     3301 cttcctgggc acgatcaccc gctggaacga cagccgcatc gccgcgctca accccggcat
     3361 cacgctccct gaccaggcca tcacgacggt ccaccgttca gacggttccg gcacaacctc
     3421 gatcttcacc aactaccttt ccatggctgt gccaacctgg aagtcagagg tcggcgcggg
     3481 gaaatcggtc
//
LOCUS       DATMZD010000262         2505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_47483,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000262 DATMZD010000000
VERSION     DATMZD010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47483"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="VN478_05425"
     CDS             complement(<1..550)
                     /locus_tag="VN478_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017753278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HWQ22147.1"
                     /translation="MGIFNLHAPFEPSGDQPEAIAKLIAGINEGDRFQTLVGVTGSGK
                     TFTMANVIKEFDHPVLILSHNKTLAAQLYSEFKAFFPENAVEYFVSYYDYYQPEAYVP
                     TIDLYIAKDADINQDIARLRHSTIRSLIERKDVIVVSSVSCIYGWDSPEEYREGLLTV
                     TQGAAMKRDDIARSLIGLRYERV"
     gene            complement(668..>2505)
                     /locus_tag="VN478_05430"
     CDS             complement(668..>2505)
                     /locus_tag="VN478_05430"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HWQ22148.1"
                     /translation="GTLTYTFQWQRSANGGTTFVDIPGATDAVRLLTPADNLCLTRLE
                     VTCTDDGQGLPLHQSTVAWSGPVMILNASPVITEGLMVTAVCDEDESPASFGLTLHAT
                     DPDAIDTLTWSIATMPGHGSLTLPVVPVGASLVLVYHPAANWHGVDTFRLRVEDGLTG
                     SGECTVTVVVNPRNDPPVNTAVPVVVGAMISESRVHCTDGVWNDGIDLVPGSLTCTYQ
                     WVRARSDTGRALEDIPGATSSVFILRNVDIGWYVGARVTFTDTGEGLPLQQSAFVLTE
                     LHAVVAAGTTPPSITFPSFATIPGVTNCSGTTVPSFTTTEPSFALSFTIEDDSPKSVH
                     WTLAIGDRTLFATDGAGSFTRVLSLAEGLNDVEITAEDASGNQACRHLHITLDTHAPE
                     VTLVEEVPQSISSSLLILAGSIHDDLSGLRQFSINGEQVIPYADGTFRESLTLKQGEN
                     TVLITTMDNAGNRGSRTVRVICTIPVSPKVSSHTMTLVIGSGTMTVDGSTVSLDSPAV
                     LREDRTLVPLRAIVEHLGGTVAWNAKTRQVTVKARGVTIVLTIGRNTATVNGKTCTID
                     PKNAKVVPVLSYSRTLLPLRFVAEQLGLEVAWDAGTRTITLTWTD"
BASE COUNT          486 a          714 c          835 g          470 t
ORIGIN      
        1 cgacacgttc gtatcggagt ccgatgagcg agcgggcaat gtcgtcgcgc ttcatggcag
       61 cgccctgggt caccgtcagc aaaccctctc gatactcttc cggggaatcc cagccgtaga
      121 tgcagctgac ggagctgacc acgatcacgt ccttgcgctc gatgagcgaa cggatggtcg
      181 aatgacgcag gcgggcgatg tcctggttga tgtcggcgtc cttggcgata tagaggtcga
      241 ttgtcggaac gtaggcctca ggctggtagt agtcgtagta gctgacgaag tactcgacgg
      301 cattctccgg gaagaaggcc ttgaactcgc tgtacagctg tgctgccagc gtcttgttgt
      361 gactgaggat caggaccggg tggtcgaact ccttgatgac gttcgccatc gtgaacgtct
      421 tgcccgaacc ggtgacgccg accagggtct ggaaacgatc accctcattg atgccggcga
      481 tcagcttggc gattgcctcc ggttggtctc cggagggctc aaacggtgca tgcagattga
      541 agatacccat agaattcagt ataggcagat gcgccattga cgccagcgtg attccgttct
      601 tctgctctca agaccagaag ggcccgccgt gaaggcgggc ccggtggacg tgaagagggc
      661 agggagatca gtcggtccag gtgagagtga tggtccgggt ccctgcgtcc caggcgactt
      721 cgaggcccag ctgctcggcg acgaagcgga ggggcaggag cgtccggctg taggagagga
      781 ctgggacgac cttggcgttc ttcggatcga tggtgcaggt cttgccattc acggtcgccg
      841 tgttccgccc gatcgtgagg acgatggtca caccgcgcgc cttcactgtg acctgacggg
      901 tcttcgcatt ccaggcgacg gttccgccca gatgttccac aatggctcgg agcgggacga
      961 gtgtccgatc ctcccggagg acagccggcg agtcaagcga gaccgtgctg ccgtccaccg
     1021 tcatggtccc gctgccgatc accagagtca tggtgtggga ggagaccttc ggagagacgg
     1081 ggatcgtgca aatgacgcgg acggtccggg atccccggtt gccggcgttg tccatggtgg
     1141 tgatcagcac cgtgttctcc ccctgcttca gggtaaggct ctcccggaag gtaccgtctg
     1201 cgtacgggat gacctgctcc ccgttgatgc tgaactggcg cagaccggag aggtcatcgt
     1261 ggatggatcc ggcaaggatg agcagggaac tggagatact ctgcgggacc tcttccacaa
     1321 gcgtaacctc gggggcgtgt gtatcaagcg tgatatgcag atgacggcag gcctggttgc
     1381 cggaggcgtc ctctgccgtg atctccacgt cgttcagacc ctcggcgagt gacagcacac
     1441 gggtgaagga accggcgcca tccgtggcaa acagggtgcg gtccccgatg gcaagagtcc
     1501 agtggacact cttcggagag tcgtcttcaa tcgtgaatga cagggcaaag gacggctctg
     1561 tcgtggtgaa cgacggtacg gtggtcccgg agcaattggt tacgccggga atcgtcgcga
     1621 agctggggaa ggtgatggaa ggcggggtcg tgccggctgc gacgactgca tgcaactcag
     1681 tcaggacaaa tgcgctctgc tgcagcggaa ggccttcgcc ggtatcagtg aaggtgacgc
     1741 gtgcgcccac ataccagccg atgtccacgt tgcggaggat gaacactgac gacgtggcac
     1801 cggggatgtc ctccagggcc ctgcccgtgt cactcctggc acgcacccat tgataggtgc
     1861 aggtgaggga accaggaacc agatcaatgc cgtcgttcca gacaccgtcc gtgcagtgga
     1921 ctctgctttc ggagatcatc gcgccaacga cgacgggaac cgccgtgttg acagggggat
     1981 cgttgcgggg attcacaacg acggtcaccg tgcactcacc ggagccggtc agaccgtctt
     2041 ctacccgcaa ccggaacgta tcaaccccgt gccagttcgc ggcagggtga tacaccagaa
     2101 caagagatgc cccgactggg acaacaggca gtgtcaggct cccatgacca ggcatcgtcg
     2161 cgatgctcca tgtcagggtg tcgatcgcat cgggatccgt cgcatggagc gtcaggccga
     2221 aggaggcggg actttcgtcc tcgtcacaca cagccgtcac catcaggccc tctgtgatga
     2281 cgggcgaagc attgaggatc atcacgggtc ctgaccacgc cacggtcgac tgatggagcg
     2341 gcaggccctg gccgtcgtca gtacaggtga cttcgaggcg agtcaggcag aggttatcgg
     2401 caggggtcag gaggcggacc gcgtcagtcg ccccggggat gtccacgaac gtcgtgccgc
     2461 cgttcgcgct cctctgccac tggaacgtat aggtcaaggt ccctg
//
LOCUS       DATMZD010000263         3206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_27654,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000263 DATMZD010000000
VERSION     DATMZD010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27654"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..1136)
                     /gene="asd"
                     /locus_tag="VN478_05435"
     CDS             complement(60..1136)
                     /gene="asd"
                     /locus_tag="VN478_05435"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011019757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HWQ22149.1"
                     /translation="MKRTRVAVCGATGMVGQIYVAMLADHPWFELVGVAASERSAGNP
                     YEDVARWYIQGEIPTGARSMTVLPCTAKAIAATGATLVFSALPSDVAGPIEQSLADAG
                     MVVVADTASHRMDVDVPLIIPEVDADHLEALSVQRKGGKRGFIVTGPNCSTAGLVLSL
                     KPLQTAFGIRRVVVVTMQALSGAGYAGVPSMAIVDNLIPYIRGEEQKMERETRKMLGS
                     WLDGSFVPADIPVSASCNRVAVLEGHTESVLVELGRPASPEEVGSVMHDFQGEPQRLH
                     LPSAPDHPIIVRSEPDRPQPRLDRMAGEPARAHGMAAVVGGIRADTAFETGIKYTVLS
                     HNTIRGAAGNAILTAELLVAKGLL"
     gene            complement(1325..1897)
                     /gene="pyrE"
                     /locus_tag="VN478_05440"
     CDS             complement(1325..1897)
                     /gene="pyrE"
                     /locus_tag="VN478_05440"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003364258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HWQ22150.1"
                     /translation="MTTDELVMNELKETGALLEGHFLLSSGRHSDRYVQCARLLQYPD
                     RAARVLAVAAEQFRAVPFDLIVGPAMGGIIVAYELARQLGKPGIFVERENGAMSLRRG
                     FAIAEGTRCIISEDVVTTGKSSEETAQVIRDAGGEVVGIACIVNRSAELIPHPVWSAI
                     RLTIESWLAGDCPLCSHGIPWVKPGSRGNA"
     gene            complement(1941..2840)
                     /locus_tag="VN478_05445"
     CDS             complement(1941..2840)
                     /locus_tag="VN478_05445"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HWQ22151.1"
                     /translation="MLETTLCGIALKNPVIAASGTFGFAEEYGRLIDVSRLGGVCTKG
                     LTLLPREGNAGLRVWETPAGMMNSVGLQNPGIRVFIERELPRMRTLGTAVIANVGGGD
                     IEEYVDGVRLVSQQDGVDIIELNISCPNVHEGGMAFGIKCDAAATVVAAVRTVCTKPM
                     MVKLSPNAEDVVAMAVTCERAGADAVSLVNSFKALAVDVRTRGPVFENITAGLSGPAI
                     KPIALRMVWEVAHAVHIPVVGLGGISTWQDALEFIMAGATAVQVGTATFVKPTTMQDI
                     VDGLEHYCAEERLANISVVRAIL"
     gene            complement(2833..>3206)
                     /locus_tag="VN478_05450"
     CDS             complement(2833..>3206)
                     /locus_tag="VN478_05450"
                     /inference="COORDINATES: protein motif:HMM:NF021903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HWQ22152.1"
                     /translation="TGCKVTVIAGFRHEAYAVSEFPLYGAVVMVATEDGCQGRTGLCT
                     DIFDPAMFDTVFTCGPMPMMAHVAQMCADADVPCQASLEAHMACGVGACLVCSCPTVH
                     GNKRVCADGPVFDTQEVIWHA"
BASE COUNT          573 a         1016 c         1033 g          584 t
ORIGIN      
        1 gtgcatgata gcggcggcat cctgcacgag gacaccgccg aacttcatca cgacggtctt
       61 cacaacaggc ccttcgccac cagcagctcg gcggtcagga tggcattccc ggcagccccg
      121 cggatagtgt tgtgagacag gaccgtgtat ttgatacccg tctcgaaagc ggtatcggcg
      181 cggatgccgc cgacgacggc ggccatgccg tgggcccggg ccggctcacc cgccatgcgg
      241 tcgaggcggg gctggggacg gtcaggctcc gaacgcacga tgatcgggtg gtccggagcg
      301 ctggggaggt gcaggcgctg cggctctccc tggaagtcgt gcatgacgct gccgacctcc
      361 tccggcgagg ccggacggcc tagttccacg aggacggact cagtgtgtcc ttccagcacg
      421 gcgacgcggt tgcaggaagc gctgaccggg atgtcggccg gcacgaacga cccgtccagc
      481 cacgagccga gcatcttccg ggtctcgcgc tccatcttct gctcttctcc cctgatatag
      541 gggatgaggt tgtcgacgat ggccatcgac ggcaccccgg catatcccgc gcccgacagc
      601 gcctgcatgg tcacgacgac gacccttcga ataccgaacg ctgtctgaag gggtttgagc
      661 gacagaacca gtcctgcggt gctgcaattg gggccggtga cgatgaaccc acgcttgccc
      721 cccttgcgct ggaccgagag tgcttccagg tggtcggcgt cgacctcagg gatgatgagc
      781 gggacgtcga cgtccatgcg gtgtgatgcc gtgtccgcga cgaccaccat ccctgcgtct
      841 gcaagcgact gctcgatggg ccctgcgacg tcgctgggca gggcggagaa gacgagcgta
      901 gcgcccgtcg ccgcgatagc cttggcggta caggggagga cggtcatgct gcgggcgccc
      961 gtggggatct ctccctggat gtaccagcgt gcgacatctt cgtacggatt gccggccgac
     1021 cgttcgctgg cggcgacgcc aaccagctcg aaccaggggt gatccgccag cattgcgacg
     1081 tagatctgac cgaccatgcc tgttgcccca catacggcga ctctcgttcg tttcatcgtt
     1141 gtcccctttg ccacggatcc cccggggatt gcgccgggga gttgagaggt agcgtagcgg
     1201 agtggacgct tctgtcaacc acagactgta gcagacgtct cctatttccg agatcgttca
     1261 ggaccaggga gatccagagg cacagccccc tccaggtggg aagaggcttg ttcagtacgt
     1321 aagatcaggc gtttcccctg cttcccggct tcacccatgg gataccatgg ctgcacagag
     1381 ggcagtcgcc ggccagccag ctctcgatcg tgaggcggat ggcgctccag actggatgag
     1441 ggatcagttc ggcgctgcga ttgacgatgc atgcgatgcc gaccacttcg ccaccggcat
     1501 ctcggatgac ctgggcggtc tcttccgaag acttgccggt cgtgacgacg tcctcgctga
     1561 tgatgcagcg agtcccttcg gcgatggcga agccacggcg caaggacatg gcgccgttct
     1621 cgcgctcgac gaagatgccg ggcttgccca gctgtcgcgc cagctcatag gccacgatga
     1681 tgccacccat ggccggcccc acgatgaggt cgaacgggac agccctgaac tgttcagcag
     1741 ctacggccag cacgcgagct gcgcgatcgg gatattgcag caggcgtgca cattgaacat
     1801 agcggtcgct gtgcctgccg gacgacagca ggaagtggcc ctcgagaagg gcgccggtct
     1861 cctttagttc attcatcacc agttcgtcag ttgtcatggt atcctccgag gatggtaggg
     1921 aaatgtggtg tggtcctttg tcacaagatg gcgcgtacga ccgagatgtt ggcgaggcgc
     1981 tcctccgcgc agtagtgctc cagtccgtcc acgatgtcct gcatggtcgt cggcttgacg
     2041 aaggtcgctg tgccgacctg taccgcagtt gcgcccgcca tgatgaactc gagggcgtcc
     2101 tgccaggtgc tgatgcctcc caggccgacg accgggatgt ggacagcgtg tgccacctcc
     2161 cagaccatgc gcagggcgat gggcttgatc gccgggccgg acagacctgc agtgatgttc
     2221 tcgaagacgg gcccccgcgt gcggacgtcg acagccagcg ccttgaatga attgaccagc
     2281 gaaacggcgt cggctcctgc acgctcacag gtgacagcca tcgcgacgac gtcctctgca
     2341 ttgggcgaga gcttgaccat catgggcttg gtgcacacag tgcgcactgc cgctacgact
     2401 gtcgcagccg catcacactt gatgccgaag gccatacctc cctcatggac gttcgggcag
     2461 ctgatgttga gttcgatgat gtccacgccg tcttgctggg acacgagtct gacgccgtcg
     2521 acgtactcct cgatgtcacc gccgcctacg ttggcgatga cggctgtccc cagcgtgcgc
     2581 atgcggggca gctcgcgctc aatgaacacg cggatgccgg gattctgaag ccccactgag
     2641 ttcatcatgc ctgcaggcgt ctcccagacg cgcagaccgg cgttgccctc gcgcgggagc
     2701 agggtcaggc ccttggtgca gacgccgccc agcctgctga cgtcgatcag ccttccgtat
     2761 tcctcagcga agccgaatgt gccggacgcc gcgatgacag ggttcttgag ggcaatgccg
     2821 cacagcgtcg tctcaagcat gccagatcac ctcctgtgtg tcgaacactg ggccgtctgc
     2881 gcaaacgcgc ttgttgccat ggactgtcgg acacgaacag acgaggcagg cgccgacgcc
     2941 gcaggccatg tgcgcttcga gcgatgcctg acaggggacg tcggcgtcgg cgcacatctg
     3001 tgcgacatgc gccatcatgg gcatggggcc gcacgtaaag accgtgtcaa acatggcggg
     3061 gtcaaagatg tcggtacaca gtcccgtcct gccctgacat ccgtcctcgg tggcgaccat
     3121 tacaactgcc ccgtagaggg ggaactcgga taccgcgtac gcctcgtgac gaaaacccgc
     3181 tataaccgtg accttgcagc cggtca
//
LOCUS       DATMZD010000264         4121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_77031,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000264 DATMZD010000000
VERSION     DATMZD010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4121
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77031"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="VN478_05455"
     CDS             complement(<1..498)
                     /locus_tag="VN478_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWQ22153.1"
                     /translation="MFGNMKKILALALVVAMTVVLVSGCAPKPKPVVALTLWGSQDDQ
                     AMLQTMADSFKAAHTDKDYTITLGVCGEDVAKDTVLKDLDAAADVFAFASDQIAALQS
                     AGALLPVTINTDAIKAANSPASVTASTVDGQLVAYPSSADTYFLYYDKKYVSDAEAGS
                     LEAIMG"
     gene            complement(844..1416)
                     /locus_tag="VN478_05460"
     CDS             complement(844..1416)
                     /locus_tag="VN478_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22154.1"
                     /translation="MLFDTLLVIARALNQADVHWGVGASVLLYYHGLVDAPHDIDIMI
                     SEADADMVASILGTLGREAPGDPARSLYSTSRFLEYVVDGTDIDVMAGFAIRHDDGTY
                     VFPFDERSVTMRKSVDGVTIPFSSLEDWYVLYQLMPGREAKVKLIEDHIRSDGIADEV
                     LLRRALAAGLPSDVRARTERLLASCHPSGR"
     gene            1577..2587
                     /locus_tag="VN478_05465"
     CDS             1577..2587
                     /locus_tag="VN478_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 43"
                     /protein_id="HWQ22155.1"
                     /translation="MTTQEFLSLDWHLLKENPLIGPPGRLTGPIIADPTFVGPDASPD
                     GKWHLFAHTIHGIEQFVSADGQEWRHVAQVTKRAMRAFLVAVGGTYHLFYEQPRFQYA
                     DIMFLFHVRWRSRIVRISSTDLIHWSAPVSVLEPSLPWHMDNPYGASVSNPCVVEAGG
                     KWMLYYSAGLTHVADCGFEEPTHIGAAVADDVTGTYVPIPEPLLSPDLDLPFCNLCCG
                     SIKVMKLDDGFVGMQNGIYTDKDGRSGSALLRLTSTDGLHFVYVDRERPFFGPSDRGG
                     GWMRSHVYACDFRPVPAVNAWYLYFNARNDWFWTRGRERIGCLTASMQPQDTSTEERN
                     RE"
     gene            2580..3851
                     /locus_tag="VN478_05470"
     CDS             2580..3851
                     /locus_tag="VN478_05470"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWQ22156.1"
                     /translation="MSRFSVKTWSALVLLGLAGQLAWVVENLWFNGYVTAMAGARAVD
                     AIAWMVAVSAATATLTTMVMGTLSDRLRRRKPLIVLGYVVWGITTALFPFVTALPSSL
                     LVMGIVIADAVMTFFGSTANDAAFNAWVTDITPSVRRGRVEGVLSALPLFAIIITSVM
                     AGVMTRTTGEGYVPFFFVIGGFVTASGLASMFLIKDDPRLVGMGTGADEKFTAHFFKV
                     FRLSTIRQHPELFLVLSAIFLTGFGDQIYTPYVIPYMTGYLGLTQSEFTMILASGMLG
                     GGILSFFLGVLADKFDKRRLALVTPILQALAMFSMSLAHGTVQLILAFLVMTATTTFS
                     AIVITSMARDLMPPDQVGQFQGVRMIFYVALPMMTGPFVGNYVIKTLGVFRDGQYLPK
                     PSLFAVAAGAMLLTMIPVIILNLRRRIPQTA"
     gene            3876..>4121
                     /locus_tag="VN478_05475"
     CDS             3876..>4121
                     /locus_tag="VN478_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HWQ22157.1"
                     /translation="MVGQIVTRWAREVDPHAPLQEYPRPQLVRPDWLNLNGLWSYAVT
                     KGEPPVNYEGLILVPFPIESSLSGVGRALLPQETLWYE"
BASE COUNT          765 a         1257 c         1200 g          899 t
ORIGIN      
        1 gcccatgata gcctcaagtg agccggcctc agcgtcagac acgtacttct tgtcgtagta
       61 gaggaagtag gtatcggcag aggacggata tgccaccagc tgaccgtcga cagtggatgc
      121 tgtcaccgac gcgggcgagt tggccgcctt gatcgcgtct gtgttgattg tgaccggcag
      181 aagggctcct gcgctctgca gcgccgcgat ctggtcgctg gcgaaggcaa agacgtccgc
      241 cgcagcgtcc aggtccttca ggaccgtatc ctttgcgacg tcttcaccgc agacgccaag
      301 cgtgatcgtg tagtctttgt cagtgtgtgc cgccttgaat gagtcggcca tcgtctgcag
      361 catcgcctga tcgtcctgcg atccccacag cgtgagtgcg accaccggtt ttggttttgg
      421 agcgcagccg gaaacgagta cgactgtcat ggcaacgacc agagcgagcg caagaatctt
      481 cttcatgttg ccgaacatgt atcctccctt tctctgtttg tcagagcatg tggatgaatc
      541 acgggttcgc actttctgaa gaatctcgac agcgggtgtc cagtcgacac gataagctgg
      601 aacagcaggt gttctaattg acgacttgtt ctcacctccc ctgcatgaat ccacccctga
      661 tcctcgcaac ggcaatacgt cccaccagcg aagtcacaag gtccaccacc tgactcctgt
      721 tatagccaca atcgtttttc ggtcaaacaa ggataccggt cgcgaaaacg tgagagggct
      781 cgtgtccgtc actctcatgc agacgtaagg acgtgggttc ccgacttagc gaagacgaca
      841 ggtctagcgc cctgatgggt ggcaggacgc caatagcctc tcggttctgg cgcggacgtc
      901 ggaaggcagt cccgcggcaa gcgcccgtct gaggagcact tcgtcagcaa tgccatcgct
      961 ccgaatgtgg tcttcgatca gcttcacctt ggcctctctg cccggcatca gctggtagag
     1021 cacataccag tcttccagcg atgagaacgg gatcgttaca ccatccacgc tcttccgcat
     1081 cgtgacagac ctttcgtcaa acggaaacac ataggtgcca tcgtcgtgcc tgatggcgaa
     1141 tccggccatg acgtcgatgt ctgtcccgtc gaccacgtat tcgaggaacc ggcttgtcga
     1201 gtagaggctt ctagcggggt caccgggcgc ttcacggccc agagtgccga ggatcgaggc
     1261 gaccatgtct gcgtccgcct cactgatcat gatgtcgatg tcatgtggag cgtcgaccag
     1321 accgtggtag tacagcagta ccgatgcgcc gacgccccag tgaacgtcag cctgattcag
     1381 cgcacgtgcg atcaccagca gcgtatcgaa cagcacatcc cgcctcctgt catgagtgtc
     1441 ccttgtctgc gacaagcaca cggttcagta tagcggtctc gtggccaacg tgtgcgttcc
     1501 cgccttcgcc agggaggggg ggcgtgctgt ccgctgggtg attgactgtc gcacggggct
     1561 gcaatacact tcctgcatga ctactcagga gtttctttct ctggactggc acttgttgaa
     1621 ggagaatcca ctgattggtc cccccggccg gctgaccgga cccatcatcg ccgacccgac
     1681 gttcgtcggc cccgatgcgt ctccagacgg caagtggcac ttgttcgcac acaccatcca
     1741 cggcatcgaa cagttcgtct cagcagacgg acaagagtgg aggcacgtcg ctcaggtgac
     1801 gaaacgcgcg atgcgcgcct tcctcgtcgc tgtgggcgga acgtatcatc tcttctacga
     1861 gcagccacgc ttccagtacg ccgacatcat gttcctgttc catgtccgat ggcgttcgcg
     1921 tatcgtgcgc atttcgtcga cagatctcat tcattggtct gcacctgtgt ctgtgctcga
     1981 accgtcgctt ccctggcaca tggacaaccc ctacggagcg tccgtgtcca acccctgcgt
     2041 cgtcgaagcg ggcggcaagt ggatgctgta ctacagcgca ggcctcaccc atgtggcgga
     2101 ctgcgggttc gaagagccga cgcatatcgg ggcggctgtt gcggacgacg tgaccgggac
     2161 gtacgtcccg atacccgagc cattgctgtc gcctgatctc gacctgccgt tctgcaactt
     2221 gtgctgtggg tcgatcaagg tgatgaagct ggacgacggt ttcgtgggca tgcagaacgg
     2281 catctacacg gacaaggacg ggaggtcggg gtcggcgctg ctgcgcctca cgtccacgga
     2341 cggcctgcac ttcgtatacg tggaccgcga acgtcccttc ttcggtccca gtgatcgagg
     2401 aggtgggtgg atgaggtcgc acgtgtacgc ctgtgacttc aggcctgtcc cggcagtgaa
     2461 tgcctggtac ctgtatttca acgcgcgcaa tgactggttc tggacgcgtg gccgtgaacg
     2521 tatcggatgc ctgacggcca gtatgcagcc gcaggacact tcaactgagg agcgcaatcg
     2581 tgagtagatt cagcgtgaag acgtggtcgg ccctggtcct gctgggactg gcgggacagc
     2641 ttgcatgggt tgttgagaac ctctggttca acggctacgt taccgccatg gcgggcgccc
     2701 gcgcggtcga tgccatcgca tggatggtcg ccgtctcagc cgctaccgcg acgctgacaa
     2761 ccatggtcat gggcactctg tcggatcgcc tgagacgccg taagcctctt atcgtccttg
     2821 gctacgttgt ctggggcatc acgacggcac tctttccgtt cgtcacggca ctcccgtctt
     2881 cgctgctggt catgggcatc gttattgctg atgccgtcat gaccttcttc ggctcgaccg
     2941 ccaacgacgc tgcgttcaac gcatgggtca cagatatcac gccatcggtc agacgcggcc
     3001 gcgtcgaagg ggtcctgtca gccctgcccc tgtttgccat catcattacg tccgtgatgg
     3061 ctggcgtcat gaccagaacc accggcgaag gctacgtccc cttcttcttc gtgatcggcg
     3121 gctttgtcac cgcctcaggt ctcgccagca tgttcctcat caaggacgac ccccgactgg
     3181 tcggcatggg gacgggcgcg gacgagaagt tcacggctca cttcttcaag gtgtttcgtc
     3241 tcagtaccat cagacaacat ccagaactct tcctcgtatt gtctgcgatc ttcctgacgg
     3301 ggttcggcga tcagatctac actccgtatg tcatcccgta catgactggc tacctcgggc
     3361 tgacacaatc ggaattcacc atgatcctgg cgtccggcat gctcggcggg ggtatcctct
     3421 cgttcttcct gggagtcctc gcggacaagt tcgacaagcg ccgcctggcc ctggtgacgc
     3481 ccatcctgca ggcactggcg atgttcagca tgtcactggc acacggtacg gtgcagctga
     3541 tcctggcatt ccttgtcatg actgccacca cgacattcag cgctattgtc atcacatcga
     3601 tggcgcgcga tctcatgccg cccgaccagg tcggtcagtt ccagggtgtc cggatgatct
     3661 tctacgtggc actgccgatg atgaccggcc ccttcgtcgg caactacgtc atcaagacac
     3721 ttggcgtctt cagggacgga cagtacctgc caaagccgtc gttgttcgct gtggctgcag
     3781 gtgcgatgct cctgactatg atcccggtca tcatcctcaa cctgagacgc cggataccac
     3841 agactgcata gatggacatg gagggctgga aaccagtggt cggccagatc gtgacccgct
     3901 gggcgcgtga agtcgacccg catgcgccgc tgcaggagta ccccaggcca cagctggtcc
     3961 gcccggattg gctgaacctc aacgggttgt ggtcgtacgc cgtgacgaag ggcgagccac
     4021 ctgtcaacta cgaaggcctg atcctggtac cgtttcccat cgagtcatcg ttgtccggcg
     4081 ttggtcgtgc tctgctcccg caggagacac tgtggtatga g
//
LOCUS       DATMZD010000265         7394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_18219,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000265 DATMZD010000000
VERSION     DATMZD010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7394
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18219"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            201..1409
                     /locus_tag="VN478_05480"
     CDS             201..1409
                     /locus_tag="VN478_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HWQ22158.1"
                     /translation="MKRLLSLLLLLMLLTVTFAGCKQTSTDPDTTAAKFHIGIVTGTV
                     SQSEDGYRGGEALAKEYGAVADGGIIDTRTWPDNFQAEQETTISNIVSLADDPLMKAI
                     IVSDAVPGTAEAFKQVRAKRSDILLFAGKPMEDPSIISSAADVAADYDQLSNAYINVW
                     AAKQMGAKSLVFVSFPRHMSLELISRSWAIAQAAGKDLGIATYYESATDPLSDVGVPG
                     AQQYILEHLSAWLQKYGKDTAFYTTNLALQEPLLKQIMQYGGVSVGILAPTLGYPGAF
                     GIDLSAEKGNWPAILKKVEETIVTKGGSGRFNAWGFSMDYVTVAGLGEYAKRCIEGTA
                     KVGNQADMLSAYEKFSPGAQWNCNVYTDVGTGKKVANYYQLYQDTYVFGKGYLGATKE
                     KVPEKYLTIK"
     gene            1458..2573
                     /locus_tag="VN478_05485"
     CDS             1458..2573
                     /locus_tag="VN478_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HWQ22159.1"
                     /translation="MEDARIRKFAQFLINRAVYLKQGEKILIELHGHAYTLAKALVEE
                     AYRAGGEPFIHVFDYELEGALIAGTDTEHMADVASYELARMRDMDAYIDVRATDNITA
                     WNSIPADQMAIYHKEYWGPLHLRQRCNHTKWSVLRYPNDAMAQLAGMSTEEYEDFYFR
                     ACLLDYDKMAKAMENMVKRMQRTDKVHITGPGTDISFSIKGIGSFAMSGNKNIPDGEI
                     YTAPVRDSVNGVISYNVPSPESGFLFHDVKLEFRHGQIIQASADNTGLINKILDTDEG
                     ARYVGEFAFGVNPVITKPMADILFDEKIAGSFHLTPGNSYDNASNGNHSAVHWDLISI
                     QRPEFGGGEIYFDDELIRKDGMFVVDDLKCLNPENLA"
     gene            2761..3858
                     /locus_tag="VN478_05490"
     CDS             2761..3858
                     /locus_tag="VN478_05490"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HWQ22160.1"
                     /translation="MIQLQRIEEVARLMSGASLDALIIGPNTDLAYLTGLTPLSDERF
                     KALVILKDGRFFHICPELYVEETREALGAKTDIFVWSDTDGFSGAVRAASQAYHLDHM
                     TIGVNDAVRAVDLLSLGEAMVASFVVGSSVLEQVRVCKGPEEKELLRTAARLADEVAV
                     EILPFIHAGAVEGDVKKKIGELLAAKGAPELAFETIVASGPNSSRPHYTGGERVITDH
                     DVVVLDFGCRFHGYCSDTSRMVFVGEPTEEQRKIYDVVLKANLAGERAAGAGVTAGEV
                     DKAARDVIEAAGYGASFLNRTGHGIGMADHETPYIKEGNPMVLREGMAFSVEPGIYLA
                     ERFGMRIEDIVLIDGGKADVLNRSPKDMVIL"
     gene            4604..5260
                     /locus_tag="VN478_05495"
     CDS             4604..5260
                     /locus_tag="VN478_05495"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HWQ22161.1"
                     /translation="MSASKKDLSARKAIERQLVADIRSPATAATPEQQNLEALRIKTF
                     VSPVRTKILIAMNMSCGESAGISVKQLSEKLGMSHGTVFYHVKQLQKAAFIEEAGTRE
                     VNGILERYYRPVKQLVRISPETAARMDIPINAYFEEIYQQTRKPVGRRSLMDEEVYVN
                     EEERNEFHERYLKLCGEYDKPGEGRMHLAAICITYEVRDDADSAASTAGGESCSDNKA
                     "
     gene            5522..6208
                     /locus_tag="VN478_05500"
     CDS             5522..6208
                     /locus_tag="VN478_05500"
                     /inference="COORDINATES: protein motif:HMM:NF025015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HWQ22162.1"
                     /translation="METLLTSLGLALLFVVLGVPLMIGKVKRNSVYGARFRATMADDR
                     VWDVVNRKCGLLLVAGGALAGVVDLLAVVGVVTRAVGAYAAGALLVYILIATVWLWRY
                     SERIAKDVGVTARDMEVGRVSPLLVALGCFAVAVAGVMSAFSTPNPWLGFRVPATFAD
                     PAVWHQVNLKAGLMLAVLSGAFGFMFLGLRGMSEGDRKRLFSGLFIGWLVSILVVAVA
                     GSLFATSLAR"
     gene            6219..6752
                     /locus_tag="VN478_05505"
     CDS             6219..6752
                     /locus_tag="VN478_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22163.1"
                     /translation="MRFGLSLTSGAIFMNVFSIGLPLLLSCVTVFSLRRIPRSQRIAV
                     SAIVVGVTLLVSALGVFFLVGELRTGVVVGERMVVETPPFVSVTVERSDILRARVVAD
                     WTQEPDLALAMRTNGTGVGTYLTGSFRLKDGRPAVLMMSGRACLIVELDGRVLMLAPD
                     DLDGLVRAIRAWGVPVS"
     gene            6770..7303
                     /locus_tag="VN478_05510"
     CDS             6770..7303
                     /locus_tag="VN478_05510"
                     /inference="COORDINATES: protein motif:HMM:NF022338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HWQ22164.1"
                     /translation="MVELTDEGRVFRPRATVWTHASTWLLAIVFTIPFMSIIFAAHDA
                     WGDLWWTVLLMGAMAAAGIAVSLNTVRVVQTMRYILTTDAVEIRAGRSLRRIPFKNVV
                     RVEVVNLAFNIVSSFRLPGLALYDVLYSDEGIVTMFSTHPLKDVVLIETSDHHKYGIS
                     PEDEAEFVNDLRSRLQL"
BASE COUNT         1569 a         2056 c         2250 g         1519 t
ORIGIN      
        1 cgtcaggcgt gaaatgccgc gaaggacgac agcgatgcgg tagcaggcgc acgcgtttct
       61 tgccgtgccg ggtcacgacg ggacagactc ccaatgatcg ggatgcccgt gtcttctgaa
      121 gaatcttgca ttattccgtg attccgctat atgtctcgta agccgaaata tggctaatcg
      181 cacgcccgga ggaacgaaaa atgaagaggc tactcagtct actgctactg ttgatgttgt
      241 tgacagtcac attcgccggc tgcaaacaga cttccacgga ccccgacact accgcagcga
      301 agttccacat cggtattgtg acgggcacgg tgtcccagag cgaagatgga taccgtggtg
      361 gcgaggctct ggccaaggaa tacggagccg ttgccgacgg tggtatcatc gacaccagaa
      421 catggcctga caacttccag gctgaacagg agacaaccat ctccaacatc gtcagtcttg
      481 ccgacgatcc tctcatgaaa gccatcatcg tcagtgatgc cgtacccggc accgctgaag
      541 cgttcaagca ggtacgcgca aagaggagcg acatccttct gtttgcgggc aagcccatgg
      601 aggaccccag tatcatttcg tcagcggctg atgtggctgc ggactatgac cagttgagca
      661 acgcctatat caatgtctgg gcggccaagc agatgggcgc caagagtctc gtgttcgtgt
      721 cattcccgcg tcacatgagc ctggagctca tcagccggtc ttgggccatc gcgcaggcgg
      781 cgggcaagga tctcgggatc gcgacctact acgagagtgc cacggacccc ctcagcgacg
      841 tcggcgtacc cggtgcccag cagtacatcc ttgagcacct ttcggcctgg ctccagaagt
      901 acggaaagga cactgcgttc tacaccacga atctggccct gcaggaaccg ctgctcaagc
      961 agatcatgca gtatggcggc gtgtctgttg ggatcctggc tcctaccctg ggctatccag
     1021 gagcttttgg catcgatctc tctgccgaga agggcaattg gcccgcgatc ctgaagaagg
     1081 tcgaggagac cattgtcaca aagggtggtt ccggccgctt caatgcctgg gggttctcca
     1141 tggactatgt gaccgtggct ggtcttggcg agtatgccaa gcggtgcatc gaaggcacgg
     1201 ccaaggttgg gaaccaggcg gacatgctgt ctgcctatga gaagttcagt cctggcgcac
     1261 aatggaactg caacgtctat acggatgtgg gcactggcaa gaaggtggcc aactactacc
     1321 agctatatca ggacacgtac gtcttcggga agggatatct gggcgccaca aaagagaagg
     1381 tgccagagaa atacctgaca atcaagtaag cggcagtctg tgacgctgct tcaacaggaa
     1441 aagagaggcg agtctatatg gaagatgcaa gaataaggaa gttcgcccag tttctgatca
     1501 atcgggcggt ctacctcaag cagggcgaga agatcctcat tgagctgcat gggcacgcgt
     1561 ataccttggc caaagccctg gtcgaagaag cctatcgtgc cggtggcgag ccgttcatcc
     1621 acgttttcga ctacgaactg gagggagctc tcatcgccgg gaccgacacg gaacacatgg
     1681 ctgatgtagc gtcttacgag ctggcacgca tgagagatat ggacgcctac attgacgtgc
     1741 gagctacgga caatatcacg gcatggaaca gcatccctgc ggaccagatg gccatctacc
     1801 acaaagagta ctggggtccc ctgcacctgc ggcagcgatg caaccacacg aagtggtcgg
     1861 tcctgcggta cccgaatgac gccatggccc aactcgccgg catgagtacc gaggaatatg
     1921 aagacttcta cttcagggct tgtctgctgg actacgacaa gatggcgaaa gccatggaga
     1981 acatggtcaa gcgcatgcag aggacggaca aggttcacat taccgggccg ggtaccgaca
     2041 tcagcttttc gatcaagggt atcggctcgt ttgccatgag tggcaacaag aacatcccgg
     2101 acggtgagat ctacacggcg ccggttcgcg attccgtaaa cggcgtcatc agctacaatg
     2161 ttccatcgcc ggagagcggc ttcctgttcc atgacgtgaa gctcgagttt cgacatggac
     2221 agatcattca ggccagtgcc gacaacaccg ggttgatcaa caagatcctc gacactgacg
     2281 aaggggcacg ctatgtagga gagtttgcct tcggcgtcaa cccagtcatc accaagccca
     2341 tggcagacat tctgtttgac gagaagatag ccggcagttt ccacctgacg ccggggaaca
     2401 gctacgacaa cgcatccaat ggcaaccact ccgctgtcca ctgggatctg atcagcatcc
     2461 agcgacccga attcggtggt ggagagatct acttcgacga cgagctgatt cgcaaggatg
     2521 ggatgttcgt cgtcgacgac ctcaaatgtc tcaacccgga gaacctggcc tgacgacgta
     2581 gccgatccag ggttcgcagt catctcgaga ggcctttctg cagggaaagg cctctcggat
     2641 gttcgtttgt cacgactgga caatgcgatg agggatcacg ttccaggctg actccatggg
     2701 gccggtctgc tgatggggtg gtggaacgcg cgacaagcac ccgtgaaagg agcaacgaat
     2761 atgatacagc tgcaaaggat cgaggaagta gcacgactga tgtccggagc atctcttgat
     2821 gcactcatca ttggaccgaa caccgatctt gcatacctga ccggtctgac acctctgtcg
     2881 gacgagcgtt tcaaggcact tgtcattttg aaggacgggc gcttcttcca catttgcccc
     2941 gaactgtacg tggaggagac ccgcgaggca ctcggcgcca aaacggacat ctttgtctgg
     3001 agcgacacag atgggttctc tggtgcggtg cgggctgcca gccaggccta tcacctggat
     3061 cacatgacca tcggagtcaa tgatgccgtc cgggccgtgg acctgctgag tctgggtgaa
     3121 gccatggtgg catcgttcgt ggtcggcagc agcgtcctcg aacaggtacg ggtctgcaag
     3181 ggaccggagg agaaggaact cctgcgcacg gctgcacggc ttgcggatga ggtggcggta
     3241 gagattctgc cgttcatcca tgccggagcc gtcgaagggg acgtcaagaa gaagatcggg
     3301 gaactccttg ctgccaaagg ggcaccggaa ctggccttcg agacgatcgt ggcgtcaggt
     3361 cccaacagct cccggccgca ctacaccggg ggcgagcgcg tcatcactga tcatgacgtc
     3421 gtggttctgg actttggctg tcgctttcac ggatactgct ccgatacctc acgcatggtg
     3481 ttcgtcgggg aaccgacgga ggagcagcgc aagatctatg acgtcgtcct gaaggccaac
     3541 ctggccggcg aacgggctgc cggtgcaggt gtgacggctg gcgaggtgga caaggccgcg
     3601 cgggacgtca ttgaggctgc cggctatggc gccagctttc tcaatcgtac cggccacggg
     3661 atcggtatgg cggaccacga gactccctat atcaaggaag gaaaccccat ggttcttcgg
     3721 gaaggcatgg ctttcagcgt cgagcccggc atctatctgg cagaacgctt tggcatgcgc
     3781 attgaagata tcgttctcat cgacggtggc aaggctgacg tgctgaacag atcgcccaag
     3841 gacatggtca tcctctagac ggcagaaagc agtccgggag gtgacctccc ggactgggat
     3901 gctgcaggtg aggcgggctc ctcgtccact gaccccgatt caacagactg cacgaccttc
     3961 gcactttctg cctccgcctg ctgctggagg cagttgtgtc tccctgcgcg atccgggggt
     4021 gtctcctggc acgtgtaccg acaacgactg gcatgcggtc cggtctccag gggcttctga
     4081 cgtgagcgac gactcggagt tcaagcggag gcaacgtggt tcttcagaag gctcacgcgc
     4141 tcacgtgggc attcatgctc agaagacgat ccactggaca cagcagcgct ggtctcgcac
     4201 ccgactcatc ttcacaatac cttcaccatc tatgttttcg ggacttgaca agagtcagct
     4261 caatactagg atgatatcgt ccgaatagtg taagacgtca gaacctaaca agacatcttg
     4321 caagggtaag acattatgca gaactgagac gtcgcggaag actcggacgg taaggatcct
     4381 ggaggtgtac gatgagacca aagacgatgg tgcgaatgag cgaactggac cgcaatgttg
     4441 cgtcaaacaa tgtccttcgt ctgccccgct aggcagccgt gtgatagatc cactaggagg
     4501 cactcatgta tgccagaaga gttgcgacag ttgccgataa gatgacgccc gtcgaaatgt
     4561 ggtcgtttga acgcctgatc cggttccctc ggtagagaag accgtgtccg catcgaagaa
     4621 ggacctttca gcgaggaagg caattgagcg tcagctggtg gcagacatca ggtcgcctgc
     4681 caccgctgct acaccagagc agcagaacct ggaggctctt cggatcaaga ccttcgtgag
     4741 cccggtcaga acgaagattc tgatcgccat gaacatgtcg tgcggggaaa gcgcgggtat
     4801 ctcggtgaag cagctgtcag agaagctggg catgtctcat ggcacggtct tctaccatgt
     4861 caagcaactc cagaaagcgg cgttcattga ggaggcaggc acgcgcgaag tcaatggtat
     4921 cctcgagcgc tactaccgtc ccgttaagca gctcgtgcgg atctcaccag agacagccgc
     4981 caggatggac atccccatca acgcctactt cgaggagatc taccagcaga ctcgcaagcc
     5041 cgtgggcaga cggagcctca tggatgagga agtctacgtc aatgaagagg agcgcaacga
     5101 attccatgaa cgctacctta agctgtgcgg cgagtacgac aagccgggcg aaggcaggat
     5161 gcatcttgcg gctatatgca tcacgtacga ggtcagagat gatgctgact ctgctgcaag
     5221 taccgcgggc ggggagagct gctctgacaa caaggcatga gagcgtaggg ccgggatcgg
     5281 ccgcgaagaa agcagtctga gggcgaacgg gccggcgaca tagtgtcggc agagaaggcc
     5341 gccactcgcg ctgtctctct cagcgcaact cctttcctcc gggacttggc gaagagtccc
     5401 ggaggtcttc atttcctggg actgcagtag cgctcacagt gatgacgtgt atagtgttga
     5461 agagacgtga cgtgcactca cagtgtcggg gcgcagggct ccggagagga gatgaacgct
     5521 gatggaaacg cttctcacaa gtcttgggct ggcgctgctg tttgtcgtcc tcggggtccc
     5581 tctgatgatc ggcaaggtga agcgcaacag cgtgtatggc gcccggtttc gcgccacgat
     5641 ggcggacgac cgcgtctggg acgtcgtcaa ccgaaagtgc gggctgttgc ttgtcgcggg
     5701 cggtgcactg gccggcgttg tcgacctgct ggccgtcgtc ggagtggtca cgcgcgctgt
     5761 cggggcatac gctgcagggg cactgctggt ctacatcctt atcgcgactg tctggttgtg
     5821 gcgctactcc gaacggatcg caaaggacgt cggtgtcacg gcacgcgaca tggaagtcgg
     5881 cagagtctcc cccctgctcg tcgccttggg ttgctttgcc gtcgccgtcg ccggggtcat
     5941 gtcggcgttc tccacaccca atccgtggct tgggtttcgt gtgcctgcga cgttcgccga
     6001 tcctgccgtc tggcatcagg tcaacctcaa ggcagggctg atgcttgccg tcctgtccgg
     6061 tgccttcggg ttcatgttcc tcgggctgcg cggcatgtcc gaaggcgaca gaaaacgcct
     6121 gttctcggga ctgttcatcg gctggcttgt ctctatcctt gttgtggcgg ttgcgggctc
     6181 actgttcgcg accagtcttg ctcgctgagg aggtctccgt gcgtttcggt ctttcactca
     6241 cgagtggcgc catcttcatg aatgtgtttt ccataggttt gccactgctc ttgtcctgcg
     6301 tgacagtatt cagcctgagg aggatcccac ggtctcagcg tatcgccgta tccgcgatcg
     6361 tcgtgggcgt caccctgctc gtcagtgcac ttggcgtgtt ctttctggtc ggcgaacttc
     6421 gtaccggagt cgtcgtcgga gagcggatgg tcgtcgagac gcctccgttt gtctcggtca
     6481 ctgtcgagcg gagcgacatt ctgcgagctc gtgtcgtggc cgattggaca caggaaccgg
     6541 atctggcgct ggctatgagg accaacggta ccggtgtcgg cacctatctc acgggaagct
     6601 tcaggctcaa ggatggacgc cccgccgtcc tgatgatgtc tggacgcgca tgcctgatcg
     6661 tagagctcga cggcagggta ctcatgctgg cgcccgacga tcttgacgga cttgtccggg
     6721 ccatacgggc atggggcgtt ccggtttcct agccggggag gaggcacgca tggttgaact
     6781 cactgatgag ggaagagtct tccgtccccg cgcaactgta tggactcatg cgtcaacctg
     6841 gctccttgcc atcgttttca ccataccgtt catgtccatt atctttgcgg ctcatgatgc
     6901 ctggggcgat ctgtggtgga cagtcctgct catgggcgcc atggcggcgg caggcatcgc
     6961 ggtgtccctg aataccgtga gggtcgttca gacgatgcgc tacatcctga cgacagacgc
     7021 agtcgagatc cgtgccgggc gcagtctgcg tcgcataccg ttcaagaacg tcgtccgcgt
     7081 cgaggtcgtc aatctcgcgt tcaacatcgt ttccagcttc cgtcttccgg gcctggcact
     7141 atatgacgtc ctctacagcg acgagggcat tgtgacgatg ttctccacgc acccgctcaa
     7201 ggacgtcgtc ctcatcgaga cttccgatca tcacaagtac ggcatctcac ccgaagacga
     7261 agcagaattc gtcaacgatc tacgctcgcg tcttcaattg tgaagccgcc ggggtcaggc
     7321 actatggcgt tcacaattgc tccatgaggc ggcgatgtat cctgacaaca ggggagctgt
     7381 gagtggtcta ccga
//
LOCUS       DATMZD010000266         4190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_47597,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000266 DATMZD010000000
VERSION     DATMZD010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47597"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1439)
                     /locus_tag="VN478_05515"
     CDS             complement(<1..1439)
                     /locus_tag="VN478_05515"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HWQ22165.1"
                     /translation="MRRAIGLLCVAAILGTVLVPGGGGVARAVAFDEVDTIGVMPSEY
                     VGTLTLESVNTVEKDFPSAYAFPSQGVAVLPNGDVVVCDTAYGRIHVLSADLVHKRVM
                     GSLGSGRGQLQYPADVAADSSGNVYVADFFGNKIVKYGTDGSVLMEFGSEGKGAGQFE
                     GPAGITVMPDGSIWVADQLNDRLEQFTAAGTYLATVTGISRPAGMTSVGLFPYVVSGG
                     DSAVYRLTGTTAVRVLVAPGEAENLIASAADVAVDARGNIYVADRGTGDLPVPAVKVF
                     SSGGQYVRSFGQYPADMNDIQDSELLSPGGVAVAPDGMVYVMNSGFFRDAANPFGSGY
                     DAKLVEYAPGGGVVNVQDYAIDLRGRLNNPQDVAVDGKGQLWIACSSPAVSTDGQTIE
                     WNRGYVEVLDSSGNELFALMRAGSRSMQVVESVAANGSGLVYIGAQDARGGFVAVYDE
                     AGNYVRTIGAGKVDGPVDLEMASDGTLWAA"
     gene            complement(1449..>4190)
                     /locus_tag="VN478_05520"
     CDS             complement(1449..>4190)
                     /locus_tag="VN478_05520"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HWQ22166.1"
                     /translation="SHISFIYESRQAQVLDADANAAMGVNQALWTTTSAGGKKLDGTG
                     TSIGIIDTGIDYTHPDLGGAKFPNAKVVGGYDFADDDKNPADSQGHGTHVAGIAAADG
                     KVQGVAPKAKLYAYKVFSDQGGGAADGDIIAALDRSVLDHCTVVNMSLGMSGGTADTP
                     ENEAINNAVKAGVVVVAATGNSGPRNPETNWPLGSPATALNAIAVAASSDGPYPVVEI
                     VTPTGTGLENIMGSYADIAPEFKEGSTFSIVDAGYGSKADFAAANVKGKVALVERGPI
                     GAGGIYFRDKVLNAQAAGAAGVIIYNHSPGVLGMTLRVAAGDETKDYVPCIGIAQDAG
                     LDLKALTTRGLVVRFRTRSNLGTLADFSSMGPTEDFHFKPEVAAPGVTVNSTFPNDEY
                     AKLDGTSMASPGVAGAVALIKAAHPDWTPETVKLALMNTSSVLRNWQNSEVITWTLQG
                     AGRVDVPAAVDAPAVAAIATSDGTTTFQTGAILINDDTVATAATIKVRSLSDHSVTFS
                     PSFAWTMDARAGILVSVAPSSVTIAPGGTAQVTVTTTIDPATVADGSHEGMVTLESSS
                     GTLHVPYIFWRAGVQVPQQLSGLQTSTATLSPGGTMDVQFNIGYGGVRPAVDDGEEPQ
                     GSSFASQVVATVTASDGATSLGTVYRRSLLLVGDHGFVWDGRDVHGNLFLTNGDYLLR
                     TSVLESNNEPTNLQISEAAHQSVPIQVTDMAGVPVLSLSIVGGSIREGGDVTVSLIAD
                     TTTGISAIGAVVQFEPYYLSLKSVQEGDFLNEGGKRPSSFVAPVNVGSGSIAVKGVTG
                     TGDVQGHGSVCTLTFTVLHSGSTELWIGGPLATDGGRTVETIARSLAITLRSGGNVWD
                     IDGNKRVDLGDMVVLARAYGSKAGDSRYDSMADLNGDGRVDDADLQILRQHYGEVYP"
BASE COUNT          765 a         1405 c         1223 g          797 t
ORIGIN      
        1 gcggcccata gcgtcccgtc gctggccatc tcgagatcga ccggcccgtc aacctttccg
       61 gcgccgatgg tcctgacgta gttgccggct tcgtcataga cggcgacgaa cccgcccctg
      121 gcgtcctgcg caccaatata gaccagtccc gacccgttcg ccgcaactga ctcgaccact
      181 tgcatagaac gggagcccgc tcgcatcaag gcgaagagct cgttcccgct ggaatccagg
      241 acctccacgt agccccggtt ccactcgatt gtctgaccgt ctgtcgagac agccggcgaa
      301 ctgcaggcta tccagagctg gcccttgccg tccactgcca cgtcctgtgg gttgttgagg
      361 cgtccacgga ggtcgatggc atagtcctgg acgttgacca ctcccccgcc tggagcgtac
      421 tcgaccagct tggcgtcgta gccactgcca aaggggttgg ccgcgtcgcg gaagaatcca
      481 ctgttcatga cgtagaccat gccgtcgggc gcaacggcga caccaccggg actaagcagt
      541 tcgctgtcct ggatgtcgtt catgtcggcc ggatactgcc cgaagctgcg cacgtactgt
      601 ccgccgctgg aaaagacctt gacagcgggg acggggaggt caccggtccc ccggtccgcg
      661 acgtagatgt taccgcgggc gtcgacggcg acgtcagcgg cgctcgcaat gaggttctct
      721 gcctctcccg gggccacaag gacgcggaca gccgtcgttc cggtcaaccg gtagacggcg
      781 ctgtcgccgc cggaaacgac atacgggaag agcccaaccg aggtcatgcc ggcgggccgg
      841 gagatcccag tcacagtcgc cagataggtg cctgccgcgg tgaactgctc gaggcgatcg
      901 ttcagctggt ccgcgaccca gatgctgccg tccggcatca ccgtgatgcc cgcgggcccc
      961 tcgaattgcc ctgccccctt cccctcagag ccgaactcca tgaggaccga gccgtctgtg
     1021 ccatatttga cgatcttgtt gccgaagaag tcggccacat agacgttgcc actactgtcc
     1081 gcagccacat cggccgggta ttggagctgc ccccgcccgc tcccgagcga tcccatgacg
     1141 cgcttatgaa ccagatcagc agagagaacg tggatccggc cgtaggcggt gtcacataca
     1201 acgacgtccc cattcggcag gacggccacg ccctgcgacg ggaacgcata ggctgacggg
     1261 aagtccttct caaccgtgtt gacactctca agggtcaatg tccccacata ttctgagggc
     1321 atgacaccga tcgtgtcgac ctcatcaaac gcgacggcgc gcgctacccc tcctccgccc
     1381 ggcacgagca ccgttccaag gatcgcagct acacacagca gtccgatagc tctcctcatc
     1441 gcagtctctt aggggtacac ttcgccatag tgctggcgca ggatctgcag gtctgcatca
     1501 tcgacgcggc cgtcgccatt gaggtctgcc atgctgtcat agcgactgtc tccagccttc
     1561 gagccatatg cgcgggcaag gaccaccatg tcaccaaggt ctacacgctt gttgccatcg
     1621 atgtcccaga cgtttccccc ggaccgcagc gtgattgcca gcgaccgggc gatcgtctcc
     1681 accgtcctgc caccgtccgt cgccagaggc cccccgatcc acagttctgt ggaaccgctg
     1741 tgcaggaccg tgaacgtgag agtacacacg ctgccatgtc cctggacatc acccgtcccc
     1801 gtcactccct tgaccgcgat actgccgctc ccgacattga caggggctac gaagctcgac
     1861 ggtctcttgc ccccctcgtt caggaagtca ccctcctgca cggacttcag ggagaggtag
     1921 tacggctcga actgcacgac agcacctata gccgaaatac ccgtcgtcgt atcggcgatg
     1981 aggctcaccg tcacgtcccc accctcgcgt atgctccctc caacgatgct cagactcagc
     2041 acggggacac ctgccatgtc cgtgacctga atcggcacag actgatgggc cgcctcagag
     2101 atctgcaggt tggtcggctc gttgttgctc tcgaggaccg acgtcctcag gaggtagtcc
     2161 ccgttggtga gaaagagatt gccatgtaca tcgcggccgt cccacacaaa accgtggtcc
     2221 cccacgagca acagcgacct tcggtagact gtcccaaggc ttgtcgcacc atcactcgct
     2281 gtgaccgtcg cgacgacctg cgatgcaaaa ctcgaaccct gcggttcttc tccatcatcg
     2341 acagccggcc gcacgccgcc atacccgatg ttgaactgga cgtccatcgt gccgccaggc
     2401 gacagcgtag ccgtactcgt ctgcaagcca ctcaactgct gagggacctg gacgcccgcg
     2461 cgccagaaga tgtatggcac atgaagagta ccactgctgg actccaacgt taccatccct
     2521 tcgtgcgagc cgtctgccac ggtcgctgga tcgatcgttg tggtcacggt aacctgtgcc
     2581 gtcccgccgg gagcaatggt cacggacgac ggagcaacgc ttacaaggat ccctgcacgc
     2641 gcatccatcg tccaggcgaa cgaaggagag aacgtcacgc tatgatccga gagactccgc
     2701 accttgatcg tcgccgccgt cgcgacggtg tcgtcattga tgagaatggc gcctgtctgg
     2761 aacgtcgtcg tgccgtcaga cgtggctatg gcggccacag ccggagcgtc gaccgcagcc
     2821 ggcacgtcga ctcggccggc tccctggagg gtccacgtga tgacttcgct gttctgccag
     2881 ttgcgaagga cgctcgacgt gttcatcagc gccagtttga ccgtctcggg cgtccagtcc
     2941 ggatgagccg ccttgatcag cgcgacggct ccagccaccc cgggcgaggc catcgacgta
     3001 ccatccagct tggcatactc gtcatttgga aaagtcgaat tcacggtgac gccgggggca
     3061 gccacctccg gcttgaagtg gaagtcctca gtagggccca tcgagctgaa gtctgcaaga
     3121 gtccccaggt tgctccgcgt ccgaaaccgg acgacaagtc ctctcgtcgt gagtgccttc
     3181 aggtccagtc cggcatcctg tgcgatgccg atgcagggga cgtagtcctt ggtctcatcg
     3241 ccggcggcaa ccctgagcgt catgccgagg acgccggggc tgtgattgta gatgatgacc
     3301 cctgcggctc ccgctgcctg cgcgttgagg accttgtccc ggaagtagat gcctccagcc
     3361 ccgatcggac ccctctcgac aagtgcgacc ttccccttga cgtttgccgc tgcgaaatca
     3421 gcctttgagc catatccggc atccacaatg ctgaacgtag acccttcctt gaactccggt
     3481 gcaatgtctg catagctgcc catgatgttc tccagcccag ttccggtcgg cgtcacgatt
     3541 tccacgaccg gataggggcc gtcgctggag gcagctaccg caatggcgtt gagcgccgtc
     3601 gccggcgaac cgagcggcca gttggtctcg gggttgcggg ggccggagtt gcctgtcgca
     3661 gcgacgacga cgacgccggc cttgacagcg ttgttgatgg cctcgttctc aggcgtgtcg
     3721 gcagttcccc cggacatgcc cagggacatg ttgacgacgg tgcagtggtc cagcaccgag
     3781 cggtcaagcg ccgcgatgat gtctccgtcg gcagctccgc caccctggtc actgaagacc
     3841 ttgtaggcat agagcttggc ctttggcgcg acaccctgca ccttgccgtc cgcagcggca
     3901 atgccggcga cgtgggtgcc gtggccctga ctgtctgcgg gattcttgtc atcgtcggcg
     3961 aagtcatacc ctcctacgac cttcgcattg gggaacttgg ctccacccag gtccggatgc
     4021 gtgtagtcaa tgccggtgtc gatgatacca atggacgttc cggtcccgtc cagcttcttc
     4081 ccgccagcag atgtcgtggt ccatagcgcc tgattcactc ccatggcggc atttgcgtcg
     4141 gcgtccagga cctgtgcctg ccgggactca tagatgaatg atatgtggct
//
LOCUS       DATMZD010000267         2470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_108277,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000267 DATMZD010000000
VERSION     DATMZD010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108277"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(463..>2470)
                     /locus_tag="VN478_05525"
     CDS             complement(463..>2470)
                     /locus_tag="VN478_05525"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWQ22167.1"
                     /translation="PVTREQFAKMIVLAKGLSEVKPGTPTFSDVPASRWSFGFVEAAA
                     KAGYVSGIGAGKFGPQAVIKRQDVAVLLIRVLGKNSEASKILEPICFANDETGISKYA
                     VGAMTIAVRPRIQLLTWDKARNLRPAEPATRGECAFAIYNSVNPVSSGKKTLVMADEE
                     YPESLFPLTSDSAYTQKEVTYLASGPISQTPYGILFPEMCTYVPSLENKHLVRNADGS
                     VITDFQLRKGMKWSDGKPVTIDDYIFSHKLYMSDAIQVVSRTPMDLVTRIEKLDTYTC
                     RVYWKSWDAFIPTGWVIYPEHILGPQFDKDPSSINTSDFTNNPVYCGPYVVKTKVEGQ
                     YMVFSSNKDWFGGEPILETITMKAITNTNTLLINLLTGQIDVSAESLPLDNARDFETR
                     MGDRFNVYYNKGTNAGIIEWNLTSNWFKDKRIRQAFYYGIDRKTVTIKASVGTDPVLS
                     PIASASVYYKPTLARYAYDPDKANALLDSAGWKWNAAHTERILPTGERALLKIPYMAG
                     ATFREREVTLIEPMLKKVGITVQHDPGDFNAMLDAATAGTFIINLHGVGFSAFDPFGS
                     VLGFRTDQIPTPANGMNGQNNYRYSSPEMDKWLNAAQVAVTSAALKEAYSHIQDIFAE
                     DLPCLYLEQRMYPDEVRKGLKGYDHFATGTVYCNWNIQYWYWWK"
BASE COUNT          483 a          655 c          757 g          575 t
ORIGIN      
        1 cttgtgatgt acttcgtggt tttcacacat tcctccccat gccgggagtg tactcccgac
       61 aattttcagt atagcacgag accgaatcgt ccacaggatg cgacaacctc agggagttga
      121 caaccagcag gagatcagcc cggcgaccat gtcttgccgc tggaaagcac agagtcacgc
      181 tatctgctgc gtgactctgt ggtgcgctga ttgatgcgct ggactggaga cacagggttg
      241 tcctgtggaa ggccgtctgc gaagaacgga gtctccagtg tctgtcagga acgagcgtca
      301 actacggaga gtatcttgcc atactgtcct gactgttcct gacgccctgg gctgtgacat
      361 cacagcccag ggtattgtgg tatcgtgtgc ctgcgaaaca cacggtgtca cctcagaagg
      421 acggaaccgt ggcaaatcct gttgcggctg tacggagatc ggctacttcc accagtacca
      481 gtactggatg ttccagttgc agtagacggt ccccgtggcg aagtgatcgt atcccttgag
      541 ccccttacgc acctcatccg gatacattcg ctgttcgagg tagagacatg gcaggtcttc
      601 ggcgaagata tcctggatgt ggctgtaagc ctctttgagg gctgctgaag tcactgccac
      661 ctgggcggca ttgagccact tgtccatttc ggggctgctg taccggtagt tgttctggcc
      721 gttcattccg ttcgcgggcg tggggatctg gtctgtccgg aacccgagga cggatccgaa
      781 gggatcgaaa gcgctgaagc caacgccgtg gaggttgatg atgaatgtgc cagcggtcgc
      841 agcgtcgagc atggcgttga agtcgcccgg gtcatgctgg accgtgatgc caaccttctt
      901 gagcatgggt tcgatcaacg tgacttcacg ctcgcggaag gtggcgccgg ccatgtacgg
      961 gatcttcagg agcgcgcgtt cgcccgtggg aagaatacgt tctgtgtgtg ccgcgttcca
     1021 cttccatcct gcgctgtcca gaagcgcgtt ggctttgtcc gggtcatagg catatctggc
     1081 gagcgtcggc ttgtaataga cggaagcaga cgcgataggt gacaggacgg ggtctgtccc
     1141 gacgctggcc ttgatcgtaa cggtcttgcg gtcgatgcca tagtagaaag cttgccggat
     1201 ccgtttgtcc ttgaaccagt tggaggtcag attccattcg atgataccgg cgttggtgcc
     1261 cttattgtaa tagacgttga acctgtcgcc catgcgagtt tcgaaatcac gggcgttgtc
     1321 cagtggaagt gactcagcgg agacgtcgat ctgccctgtc agcaggttga tgagcagtgt
     1381 gttggtattt gtgatcgctt tcatcgtgat cgtctcgagg atcggctctc cgccaaacca
     1441 gtccttgttg gaggagaata ccatgtactg accctcgacc ttggttttca cgacgtatgg
     1501 gccacagtag acagggttgt tcgtgaagtc ggatgtgttg atgctggagg ggtccttgtc
     1561 aaactgcgga cccaggatat gctccgggta gatgacccat cccgtcggga tgaaggcatc
     1621 ccagctcttc caatacacgc ggcacgtgta cgtgtccagc ttctcgatcc tggtgacaag
     1681 atccatcggg gtccggctga cgacctgaat ggcgtcgctc atgtagagct tgtgggagaa
     1741 gatgtagtca tcgatcgtga ctggtttccc atccgaccac ttcatcccct tgcgcaactg
     1801 gaagtcggtg atgaccgagc cgtcggcgtt gcggacaagg tgcttgttct ccagcgacgg
     1861 cacatatgta cacatttcag ggaagaggat gccgtatgga gtctgcgaaa tggggcccga
     1921 cgcaagatat gtgacttcct tctgtgtgta ggctgaatcc gacgtgagag ggaagaggct
     1981 ctcagggtat tcctcgtctg ccatgacaag cgtcttcttt ccggacgaga cgggattcac
     2041 cgagttgtag atggcgaagg cacactcccc acgggtagcc ggttccgcag gcctgaggtt
     2101 gcgggccttg tcccacgtga gcagctggat gcgtggacgg acggcgatag tcatcgcgcc
     2161 gaccgcgtat ttcgagatcc cggtctcgtc gttcgcaaag cagatgggct cgagtatctt
     2221 cgaagcctcg gagttcttgc cgagaacacg tatcaggagg acggcgacgt cctggcgctt
     2281 gatgactgcc tggggaccga acttgcctgc tccgatgccg gacacgtacc cggccttcgc
     2341 tgctgcctcg acaaacccga acgaccagcg ggatgcgggg acatcactaa acgtaggcgt
     2401 tcccggcttg acctcgctga gtcctttggc gagcacgatc atctttgcga actgttcacg
     2461 agtcactgga
//
LOCUS       DATMZD010000268         2809 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_148052,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000268 DATMZD010000000
VERSION     DATMZD010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148052"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VN478_05530"
     CDS             <1..358
                     /locus_tag="VN478_05530"
                     /inference="COORDINATES: protein motif:HMM:NF012832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22168.1"
                     /translation="FAEYADIRGHRYGTLVSSLEAVRPEEILVLTIDIHGGSSMKRLF
                     PEALMIFLKPPDVTELERRLECRGSETREEIAGRIELGHYEMTFAKDYDYMVTNCDLE
                     TTVDIVDSIVRSVLRR"
     gene            368..631
                     /locus_tag="VN478_05535"
     CDS             368..631
                     /locus_tag="VN478_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22169.1"
                     /translation="MDEQKEQPNQQPELANLYLETLITRVDTNKYRLIRQLLDTSHEL
                     LEEKTDPLREPVDDVLRKAAGRMVAAAARDAVTPNIKEPETGR"
     gene            721..1890
                     /gene="metK"
                     /locus_tag="VN478_05540"
     CDS             721..1890
                     /gene="metK"
                     /locus_tag="VN478_05540"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HWQ22170.1"
                     /translation="MDKNTRTSQTVEFVTIGHPDKVADQISDAILDELLTQDRYSRVA
                     CETFVTHGLVIVGGEITTNAFVDVDSVVRSTVDSIGYNHPKYGFDAKTCAVISAIGRQ
                     SPDIAQGVDAGGAGDQGIMYGYATDETPEYMPLPYQLARKLTMRQEEVRQQSILDYLG
                     PDGKSQVTLEYDGDKPVYLRSVVLSSQHLDTVLDKDNPSQMSNDAREQIIEEIVRPSL
                     PPELLDSKTAIHVNPTGKFVVGGPQSDTGMTGRKLGIDTYGGRIPHGGGAFSGKDPTK
                     VDRSATYLMRYLAKNIVAAGIAHRCLAQVAYVIGQKSPASFELSFEGSGQLPESAVRD
                     VVAQNVEMTPTGIIDYLGLRKPIYRQTSMHGHFGRPGFSWEELDLVRELKSWLKI"
     gene            1894..>2809
                     /locus_tag="VN478_05545"
     CDS             1894..>2809
                     /locus_tag="VN478_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22171.1"
                     /translation="MLGSFAVLAQRGPFSRNLLSYDLSSVGGNPEPGTWFLVPFIHDV
                     VPALLVSTSSGQGSFCETTALPLPALATCTAPGIYPEVADTVTALQHALERQVLGWDD
                     ALAIVRFLQTSQSHNRGTARLTGRDTGLLLHNLQMAAARDPLLSAKTAPMRQLLSDAE
                     VTTHLQGALWRLSTPARRKLYEQGFVVDGGLADMGERACGPLDASGEPPLLVGSFAER
                     ASRFRPAIEEAIRSGGSVALVVSSEVLASLYEGLMSVEWASPVVRWPSVSARRFRGDR
                     RSTVWLSTRQFSSVILPEVALVIVDLGIP"
BASE COUNT          582 a          829 c          827 g          571 t
ORIGIN      
        1 gttcgccgag tatgctgata tacgtggcca tcgttatggg acgctcgtgt cctcgcttga
       61 ggctgtccgg ccagaggaga ttctcgtcct gacgattgat atccatggcg gttccagtat
      121 gaaacgcctg tttccggaag ccctcatgat ttttttgaag ccaccggatg tcacggagct
      181 tgagcgtcgc ctggagtgca gaggctcgga aacacgggaa gagatagctg gacggatcga
      241 gcttggtcac tacgagatga cgtttgccaa ggactacgat tacatggtca cgaattgcga
      301 ccttgagacc acggtcgaca ttgtcgacag catagttcgc agtgtactgc ggagatagga
      361 gtcacacatg gacgaacaga aagaacagcc gaaccagcag cccgaattgg ccaatctcta
      421 tctggagacg ctgatcacca gagtcgatac gaacaagtac cggctcatcc gtcagctcct
      481 ggacacttca catgagttgc ttgaagagaa gactgatccg ttgcgggagc ctgtggacga
      541 tgtccttcgg aaggccgccg gtcgcatggt ggccgctgca gcccgcgacg ctgtcactcc
      601 caatatcaag gagcctgaga ctggacggta acccatcccg acatccgatc gattgcgaag
      661 cgacctcacc caccccgtac acgtgtgcgg ggtgtcgaca actgacgaaa ggggagacca
      721 atggacaaga acacgcgcac cagccagaca gttgagttcg tgactatcgg acatcctgac
      781 aaggttgcag accagatatc tgacgctatc cttgacgaac tgctcacaca ggatcggtac
      841 agccgcgtcg cctgcgagac attcgtcacg catggtctgg tcatcgttgg gggcgagata
      901 accaccaacg catttgtgga cgtggacagc gtcgttcgat ccacagtaga cagcatcggc
      961 tacaaccatc ccaaatatgg gttcgacgct aagacttgcg ccgtcatcag cgcaattggc
     1021 cgccagtctc ccgacatcgc gcagggagtg gacgcaggtg gcgcaggcga tcaagggatc
     1081 atgtacggct acgccacgga cgaaacgccg gagtacatgc ccttgcccta ccagttggca
     1141 cggaagctca caatgcgcca ggaagaagtg cggcagcaga gcatactcga ttacctcgga
     1201 ccagacggca agagccaggt cacactggag tatgatggcg acaagccggt gtacctccgt
     1261 tctgtcgtcc tgtcgtctca gcacctcgat actgttctcg acaaggacaa cccgtctcag
     1321 atgtcgaacg atgcgcgcga gcagatcatt gaggagattg tgcggccctc gttgccgccc
     1381 gagcttctcg acagcaagac cgcgatccac gtgaacccga ccggcaagtt cgtcgtcgga
     1441 ggtccgcagt ccgatacggg catgaccggg cgcaaactcg gcatcgacac ctatggtgga
     1501 cgtatcccac atggtggagg agccttctcg ggaaaggatc cgacgaaagt ggaccgctcg
     1561 gccacgtacc tgatgcggta tctcgcgaag aacatcgtcg cggccggtat cgcccatcgt
     1621 tgtctggcgc aagtggccta tgtcatcgga cagaagagcc ccgcctcgtt cgagctctcg
     1681 ttcgaaggca gtggccaact ccccgagtcc gccgtgcggg acgtcgttgc gcagaacgta
     1741 gagatgactc ctacgggcat catcgactat ctcggccttc gcaagcccat ctaccggcag
     1801 acatccatgc acggtcattt tggacgtccg gggttcagct gggaagaact tgacctggtt
     1861 cgagagctca agagctggct caagatatag tccgtgcttg gcagttttgc tgttctggca
     1921 cagagaggcc cattctcgcg caacctcctc tcctacgacc tcagctctgt aggggggaac
     1981 ccggaaccgg ggacgtggtt tctggttccg ttcatccatg atgtagtgcc cgcgctgctc
     2041 gtctcgacct cgagtggcca gggttcgttc tgtgagacga cagcccttcc actgcctgcc
     2101 ttggccacgt gcacggctcc gggcatatat ccggaggtgg ctgacactgt gacggcgctt
     2161 cagcatgctc tcgagcgaca ggtcctcggt tgggatgatg ctctcgcgat cgtccgcttt
     2221 cttcagacat cccagtcgca caatcgtggc accgcccgcc tcactggacg tgacacaggt
     2281 ctcctccttc acaacctgca gatggccgcg gcacgagacc cactgctgtc cgcgaagacc
     2341 gctccgatgc gccagctgct ttcggacgct gaagtcacga cccaccttca gggcgctctg
     2401 tggagactgt ccacgccagc acggcgcaag ctgtacgaac agggattcgt cgtcgatggt
     2461 gggcttgctg acatgggcga acgagcatgt ggtcccctgg acgcctcagg tgagccgccg
     2521 ctcctcgtcg gatcgtttgc tgagcgagct tcgcgcttcc ggccagcaat tgaggaagcg
     2581 atcaggagcg gtggcagcgt cgctctcgtc gtcagttcgg aagtactggc gagcctttac
     2641 gaaggactca tgtctgttga atgggctagc cccgttgtac gttggccatc agtgagtgcc
     2701 agacggtttc gcggcgaccg ccgttcgacc gtgtggctga gcacgcgtca gttctcgtca
     2761 gtgatcctcc ctgaagtcgc tcttgtcatc gtggaccttg ggattcctg
//
LOCUS       DATMZD010000269         3185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_58325,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000269 DATMZD010000000
VERSION     DATMZD010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58325"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="VN478_05550"
     CDS             complement(<1..642)
                     /locus_tag="VN478_05550"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HWQ22172.1"
                     /translation="MSFTHRYDAVLFDVDDTLLDFRASEAEACWAVVDVYAAVGVSPD
                     AFLRAFRIANDATWDLYMEKRITRAQVADRTFESLGRLLPGSERIDTHAAALTFSMAF
                     AEHSVTEPDAARVLASLHTRCVTGVISNHEQPVVQRRRLAASGLTQLLDVVVISGDIG
                     VEKPDPRIFDHALQLLDLTAKQCLFVGDSIILDDAGARNVGMDFCWYRRHHTAP"
     gene            complement(662..1666)
                     /locus_tag="VN478_05555"
     CDS             complement(662..1666)
                     /locus_tag="VN478_05555"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HWQ22173.1"
                     /translation="MNIHSMIDDRFSGAVSITQADKTIFQEAFGYADLSNRLSNRVDT
                     RFPTASAGKGFVAVGILQLIEHGGLFLDSRIGDILRFDLKEIDPHITIRQLLTHMSGI
                     PDYFDESTMTDYGELWNEFPNYRVRTSMDLLPLFIDKPMAYPAGARFRYNNAGFVVLG
                     LVIESITGQKFDAYLHDHVFAPTGMNDTGYFELDRVPERCANAYVYDESRKEYYTNIY
                     SVDAKGTGAGGAFTTILDMEHFWRSLLGGGLLSEAMTLEMTSPQIPEDCFEEGSYGYG
                     IWIDREDGRSSVLYLEGCDPGVSFRSSFDKERDLLITLVSNFGSDVWKLHEKIKAAFK
                     "
     gene            complement(1735..1827)
                     /locus_tag="VN478_05560"
     tRNA            complement(1735..1827)
                     /locus_tag="VN478_05560"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1791..1793),aa:Ser,seq:gga)
     gene            complement(1903..>3185)
                     /locus_tag="VN478_05565"
     CDS             complement(1903..>3185)
                     /locus_tag="VN478_05565"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HWQ22174.1"
                     /translation="EEARLLESELRARLDVTHSLSRSVTGLLQGRNLLVRQLDFEQRI
                     VGFVIVGIGDQRPLTVGETGAFDLFMAYAESIVQKIFVKQRSALLTKYRLAAKELFDT
                     SFKRTDLQQFLTATARKLQDVMEADASAYIPFEAESRQLVLDQADVSGFTPEPGVEPR
                     ILAEAFSSPSLAVVHSEVSNTTIPLDPGAMTELTLRLTLEGVLYGVLYISFRRPVLLT
                     DEERHMVEAFVSDCSLVAENILYVHRIDELSVTDELTGLGNYRAFVTHSVEQSERTAR
                     FGEVFSLVFFDIDDFKKYNDAYSHLDGNLALQGIADILRHSIRSVDSAYRYGGEEFVI
                     LMPGATPTDARRAAERVRASVERNSARDRHHFRTVVTLSGGVASFEDRVSDPKNLLLL
                     ADLAMYQAKKSGKNAIYVLESLATQKRTAPGSQS"
BASE COUNT          666 a          910 c          935 g          674 t
ORIGIN      
        1 cggcgccgta tgatgccggc gataccagca gaaatccatg ccgacgtttc gggcgcctgc
       61 gtcgtcaagg ataatcgagt cgccgacgaa gagacattgc tttgcggtca ggtcaagcag
      121 ttggagagcg tggtcgaaga tacgcgggtc gggtttctcg acgccaatgt ctcccgatat
      181 gacgacgaca tccaggagct gcgtaaggcc ggatgctgca agtcgtcttc tctgaaccac
      241 aggttgctca tggttgctga taacgccagt cacacatcgc gtatggagag aggcgaggac
      301 gcgggccgca tctggttcgg tgacactgtg ttcggcgaat gccatgctga atgtcagcgc
      361 ggcggcgtgc gtgtctatgc gctccgaccc cggaagcagc cggcccaggc tctcgaacgt
      421 ccggtcggcc acctgtgccc gcgtgatgcg cttctccata tagaggtccc atgtagcgtc
      481 gttggcaatc cgaaacgccc tgaggaaggc gtccggggat acgccgacag cggcatagac
      541 gtcgacaacc gcccagcatg cttcagcctc actggctctg aagtccagca gcgtatcgtc
      601 gacgtcaaag aggacggcgt cgtacctgtg tgtgaaggac atgagtcgat tcgatctgca
      661 tctacttgaa cgctgctttg atcttctcgt gcaacttcca gacgtcggac ccgaagttgc
      721 tcaccagcgt gatgagcagg tctctctcct tgtcgaagga ggaacggaaa ctcactccgg
      781 ggtcacagcc ttcaaggtag agaacggagc tcctgccgtc ttcccggtcg atccagatgc
      841 cataaccgta ggagccttct tcgaagcagt cctccgggat ctgtggggat gtcatctcca
      901 gcgtcattgc ctctgacagc agccctccac caagcaggct cctccagaag tgctccatat
      961 ccaggatcgt tgtgaatgcg ccgcctgcgc cggtgccctt tgcgtcgacg ctgtagatat
     1021 tcgtgtagta ctcttttcgg ctttcgtcat agacataggc attcgcacac ctctcgggga
     1081 ctctgtccag ttcgaagtag ccggtgtcat tcattccagt cggcgcaaag acatggtcgt
     1141 ggaggtacgc gtcgaacttc tggccggtga tgctctcgat gaccaggccc aggacgacga
     1201 accctgcgtt gttgtaacgg aacctggcgc cagcaggata cgccatgggt ttgtcaatga
     1261 acagaggcag caggtccatc gacgtacgga ccctgtagtt gggaaactcg ttccagagtt
     1321 caccgtagtc tgtcatcgtt gactcgtcga agtagtctgg tatcccggac atgtgggtca
     1381 gcagctgcct gatggtaatg tggggatcta tctctttcag atcgaatctg aggatgtctc
     1441 cgatacgact gtcaaggaag aggccaccgt gctcgatgag ctggagaata ccgacggcga
     1501 cgaagccctt tcccgccgat gctgtgggga atctcgtgtc aacccgattt gaaagtctat
     1561 tcgacaggtc agcataccca aatgcttcct ggaagatcgt cttgtcagcc tgggtgatag
     1621 aaacggctcc actgaacctg tcgtctatca tgctgtgaat gttcattgct atcctctctc
     1681 gtccgtagaa gcagcacgat gaaccgtcag atctgacgag aagatgagac ttactggcgg
     1741 agagggtggg atttgaaccc acgaggcagg ggttacccac ctactcgctc tccaggcgag
     1801 tgctttcagc cgctcagcca cctctccgca tggtagaagc acgaacacac caagtatacg
     1861 gaaaaccccc tcagttgcaa cgaaaggaga cgtaacgact gtctagctct gcgatcccgg
     1921 cgctgtgcgc ttctgggttg ccagactctc gaggacgtaa atcgcgttct tgccgctctt
     1981 cttcgcctgg tacatcgcaa gatcagcgag aagcagcagg ttcttcgggt cggacacccg
     2041 gtcttcgaag cttgccactc ctccgctcag tgtcaccacc gtgcggaagt gatggcggtc
     2101 gcgagcgctg ttgcgttcaa ccgatgcccg gacacgttca gccgccctgc gtgcatccgt
     2161 cggagtagcc cccggcatga ggataacgaa ctcctcgccg ccatatctgt aggctgaatc
     2221 gacgctgcgg atgctatggc gcaggatgtc cgcgatgccc tgaagcgcga ggttaccgtc
     2281 caggtgggag taggcgtcgt tgtacttctt gaagtcgtca atgtcgaaga acacgagcga
     2341 gaatacttcg ccaaaccgcg cagtgcgctc ggactgctcg accgagtgag tcacgaatgc
     2401 gcggtagttg ccaaggcccg tcaactcgtc ggtcaccgag agctcatcga tgcgatgaac
     2461 gtagaggatg ttctcggcga cgaggctgca gtcagaaacg aacgcctcta ccatgtgccg
     2521 ctcttcatcc gtcaagagca cggggcgcct gaacgatatg tacagcactc catagaggac
     2581 tccctcaagc gtcagacgca gagtcagttc ggtcatggcg cctggatcaa ggggaatcgt
     2641 cgtatttgag acttccgaat ggacgaccgc aagactggga ctgctgaatg cttcggccag
     2701 gatacgcggt tcgactccgg gttccggagt gaatccggaa acgtccgcct ggtcaaggac
     2761 gagctgtcgg gactccgctt cgaaggggat gtacgctgat gcgtccgctt ccatgacatc
     2821 ctgcagcttg cgagctgtgg cagtcaggaa ctgttggagg tcagtgcgtt tgaaggatgt
     2881 gtcgaacaac tccttggcag cgagccgata cttggtgagc agggcggagc gctgcttcac
     2941 aaagatcttc tgcacgatcg attccgcgta ggccatgaag aggtcgaacg caccggtctc
     3001 cccgactgtc agcggtctct ggtctccaat gcccacaatg acaaagccta cgatgcgctg
     3061 ctcgaagtcc agctggcgca ccagcaggtt gcggccctgc agcagacccg tgacagagcg
     3121 tgacagcgag tgcgtcacat ccagccgggc ccgcaactcg ctttccagga gacgagcctc
     3181 ttctg
//
LOCUS       DATMZD010000270         3864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_148124,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000270 DATMZD010000000
VERSION     DATMZD010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148124"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="VN478_05570"
     CDS             complement(<1..653)
                     /locus_tag="VN478_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HWQ22175.1"
                     /translation="MGAINGADTALVLVSAAFVFIMTPGLAFFYGGLARRKNMLSLMM
                     QSFIAMGLVALVWFFGGFSLAFGHDIGGLLGGLEYFALRGVGMAPSSVYAPTIPFLVF
                     FLYQMMFVIITPALITGAFAGRMKFGSYLVFLVLWVLLVYVPVAHWSWDVGGWMERLG
                     FVDFAGGVVVHVTAGAAAVASVFALGKRKIARNESLAPSNITYVALGVALLWFGWFG"
     gene            complement(804..1676)
                     /locus_tag="VN478_05575"
     CDS             complement(804..1676)
                     /locus_tag="VN478_05575"
                     /inference="COORDINATES: protein motif:HMM:NF024819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside adenylyltransferase
                     domain-containing protein"
                     /protein_id="HWQ22176.1"
                     /translation="MRHLSAVNDSAETSGPPDRALSDSRVPVRLRHMLGAYVDALHEA
                     FPERVYALSLYGSLALGGFVEHTSDVDFLTVMVGQISEDDQDAIKALHQKLRRVDRWA
                     SRLDGEYAELDQVEAGELDVPCLFVADGSLAGRREVSKAGWLTLIQRGVSVIGPEPAA
                     FVPDVPWVDLEQEMWSNLHSYWLPKADSRWLFLSSAWVAFAVLTLCRILFTMERHSVT
                     SKPAAAEYALGILPEEWHRLIHEALRVHSGEPGLSLYASRLARARETCRFIRSITRLC
                     DGQFGADATGVTGS"
     gene            complement(1679..2197)
                     /locus_tag="VN478_05580"
     CDS             complement(1679..2197)
                     /locus_tag="VN478_05580"
                     /inference="COORDINATES: protein motif:HMM:NF016154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HWQ22177.1"
                     /translation="MFTLTPDLWQVALRTAIVYVLVLAGLRVAGKREMGELASFDFVM
                     ILLLANAVQNAMTGPNTTVMGGIVAAATLLVINYGVSALVHRYPRLRRIILGQRTTLI
                     EQGIPQLDTLEREHIDEEEIMTAAREHDIQKLSDIRTAWLEVDGNITFQTMNGRHMRV
                     SRKKKVRYLKNQ"
     gene            complement(2210..3265)
                     /locus_tag="VN478_05585"
     CDS             complement(2210..3265)
                     /locus_tag="VN478_05585"
                     /inference="COORDINATES: protein motif:HMM:NF020933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA double-strand break repair nuclease NurA"
                     /protein_id="HWQ22178.1"
                     /translation="MKIRPDYWQSVYTGSTEVSLTDQKRSYEVVPTTECRDLDHWAAV
                     RIPGAAFAEVETVRVAFVDGVRRIDMHGIIDGNPPYYGVFGSVAAGCMQMCGSHAHDV
                     TEALCDETVRHYFVTNAPDPDTKVLKLASDTSCPFNVEYEIHTAPSHEPGDFGQELNN
                     LMQELEHDVAQQEVDRTDVRAVVTDGPIPYEHRLAAGTAKLAGIIKNVQEAYLQSAEF
                     ATVLSLGAAERTPIFEIRYKDSSGSSKMSFFLRLRAVTPFASQMANLVRVELPVMGLT
                     EAVHLANLASAIAVHYSSNLWGDTRAPQNLYPVGALETALKNRLGDQRFLRSVIMRTL
                     AEGDSALNPAPSTQSQD"
     gene            complement(3265..>3864)
                     /locus_tag="VN478_05590"
     CDS             complement(3265..>3864)
                     /locus_tag="VN478_05590"
                     /inference="COORDINATES: protein motif:HMM:NF024947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HWQ22179.1"
                     /translation="QEDLTAARRGRDDLVTRRAFLANRLKRKEEVTRLLSTYQQVARD
                     FASKGMVAFVSGGILEQLLVTANDHLRVLSKGRYELLLDEGDQMMVQDSFSASGPRDV
                     ATLSGGETFLASLALALAVKDLLSTSVDLDSFFIDEGFGTLDETTVQGVADVLESLRR
                     DGSLVGIITHRADLVERFETVLEVTNQSGSAHIARREMA"
BASE COUNT          708 a         1198 c         1244 g          714 t
ORIGIN      
        1 aagccgaacc agccgaacca cagcagcgct actcccaggg ccacgtacgt gatgttgctt
       61 ggggccagac tctcattcct ggcgatcttg cgtttgccga gcgcgaacac cgaggcgacg
      121 gctgcagctc ctgccgtcac atgcaccacc acgccgccgg cgaagtccac gaagccgagg
      181 cgttccatcc atccgccaac gtcccatgac caatgcgcca cgggcacgta gacgaggagc
      241 acccacagca cgaggaagac gaggtagctg ccgaacttca tgcggcccgc gaaggcgcct
      301 gtgatcaatg caggcgtgat gatgacgaac atcatctggt agaggaagaa gacgaggaac
      361 gggatcgtcg gggcgtagac cgagctgggc gccatgccga cgccacgcag cgcgaagtac
      421 tccagacctc caaggagtcc cccgatgtca tgcccgaatg ccagcgagaa cccgccaaag
      481 aaccagacca gcgctaccag gcccatggcg atgaagctct gcatcatgag cgagagcata
      541 ttcttgcgac gcgccagccc gccgtagaag aaggccagcc ccggcgtcat gatgaacaca
      601 aaggccgcgc tgaccaggac aagcgcagta tctgcaccgt tgatcgcacc cattcctacc
      661 tcccagaaac cggaacactg cccggacgca ttatcagtcc gagttcataa cctcatgaat
      721 catgctcaga cgtcatctgc tgtcaagtgg agaaccatga tattccagac gtgtgaggca
      781 cgtgttgctg attacagctg ctttcaggaa cctgtcactc cggttgcgtc tgcgccgaat
      841 tgcccatcgc acagccgcgt gatcgacctg atgaaccggc acgtctcccg tgcccgggca
      901 aggcgtgacg cgtagagaga cagtcccggt tcgccggaat gtacgcgcag ggcctcgtgg
      961 atgaggcggt gccactcttc aggcaggatc ccgagggcgt actcggcggc cgcgggcttg
     1021 gatgtcaccg agtgccgctc catggtgaac aggatgcggc agagcgtgag gaccgcaaac
     1081 gcaacccatg cggaactcag gaacagccag cgggagtcgg ccttcggcag ccagtaggag
     1141 tgaaggttgc tccacatttc ctgctccagg tccacccagg gcacgtccgg gacgaatgcg
     1201 gcgggctcgg gtccgatgac gctgacgccg cgctggatca gtgtcagcca tcccgccttg
     1261 ctgacctcgc ggcgaccggc aaggctgccg tcggccacga agagacaggg aacgtcgagt
     1321 tcgccagcct cgacctggtc gagttcggcg tactcgccgt ccagtcggga agcccagcgg
     1381 tcgacgcggc gcaacttctg gtgcagtgcc ttgatggcgt cctggtcgtc ctcgctgatc
     1441 tggccgacca tcacggtcag gaaatcgacg tcgctcgtgt gttcgacgaa gccccccagg
     1501 gccagcgaac catacagcga cagggcgtag acgcgctcag ggaaagcctc gtgcagtgcg
     1561 tcgacgtagg cgccgagcat gtggcgcaga cggacgggga ctcgtgagtc ggacagtgcc
     1621 ctgtccggcg ggccggacgt ctcggcgcta tcgttcactg cggacaggtg gcgcatgatc
     1681 actggttctt gaggtatcgt accttcttct tccgcgaaac acgcatgtga cgtccgttca
     1741 tcgtctggaa ggtgatgtta ccgtcgactt ccagccaggc cgtcctgatg tcgctgagct
     1801 tctggatgtc gtgttcccga gcggcggtca tgatctcttc ctcgtcgatg tgctcgcgct
     1861 ccagcgtgtc cagttgtggt atgccctgct cgatcagtgt ggtgcgctgg cccaggatga
     1921 tgcggcgcag gcgcggatac cgatgcacca gtgccgatac gccgtagttg atgacaagga
     1981 gggtcgcggc tgcgacgatg ccgcccatga ctgtggtgtt tggtccggtc atcgcattct
     2041 gcaccgcatt cgcgagcagg aggatcatga cgaagtcgaa cgacgccagc tctcccatct
     2101 cgcgctttcc cgccacgcgc aggcctgcca gaacgaggac gtagacgatg gctgtccgga
     2161 gcgcgacttg ccagagatcg ggtgtcagcg tgaacatggt tgcctccgtt cagtcttgag
     2221 actgagtaga tggtgccggg ttcagagcag agtcgccttc ggcaagcgta cgcatgatga
     2281 cgctgcgcag aaagcgctgg tcgccaagtc ggttcttcag tgcagtctcg agtgcgccca
     2341 ccgggtacag gttctgcggc gccctggtgt cgccccacag gttgctggaa tagtgcacgg
     2401 cgatggcgga ggcgaggttg gccaggtgga ccgcctcggt cagccccatg acggggagct
     2461 cgacgcggac caggttggcc atctggcttg cgaagggcgt gacggcacgg agtcgcagga
     2521 agaagctcat cttgctgctg ccactgctgt ccttgtagcg gatctcgaag atcggcgtgc
     2581 gctcggcggc cccgagcgac agcactgtgg cgaactccgc gctctggagg taggcttcct
     2641 ggacattttt gatgatgcct gccagcttgg ccgtcccggc ggcgaggcga tgttcgtagg
     2701 ggatggggcc gtccgtcacg accgcccgca cgtccgtgcg gtccacttcc tgctgggcca
     2761 cgtcgtgttc cagttcctgc atgaggttgt tgagttcctg cccaaagtcg ccaggttcat
     2821 ggctcggtgc agtatggatc tcgtattcga cgttgaacgg gcatgacgtg tcgcttgcca
     2881 gcttgagcac cttcgtatct ggatccggtg cgttggtgac gaagtagtgc cgcactgtct
     2941 cgtcgcacag tgcctcggtg acatcatgcg cgtgggaacc gcacatctgc atgcatccgg
     3001 ccgccacgga accgaagacg ccgtagtatg gcggatttcc gtcgatgatg ccgtgcatgt
     3061 cgatccggcg gacaccgtcg acaaaggcga cgcggaccgt ttcgacttca gcgaaggcag
     3121 cgccggggat ccggacggcc gcccagtggt cgaggtcacg gcattcggtg gtcgggacca
     3181 cctcgtatga acgtttctga tcggtcaagg agacctccgt ggaaccggtg tagacgcttt
     3241 gccagtagtc gggccggatc ttcatcaggc catctcccgg cgcgcgatgt gtgccgaacc
     3301 gctctggttc gttacctcga gcactgtctc gaacctctcc acgaggtcag cccggtgcgt
     3361 gatgatgccg acgagcgagc cgtcccggcg cagcgattcc aggacgtcag cgacaccctg
     3421 gactgtcgtc tcatccagtg tcccgaagcc ctcgtcgatg aagaagctgt ccaggtcgac
     3481 gctggtcgac agcaggtcct tgacggccag ggccagggca agcgaggcga ggaaggtttc
     3541 tcccccggac agtgtcgcga cgtcgcgcgg ccctgaggcg ctgaaagagt cctggaccat
     3601 catctgatcg ccctcgtcga gcaggagctc gtaccgtccc ttgctcagca cgcgcaggtg
     3661 gtcgttggcc gtcaccagca gctgttccag gatgccgccg ctgacgaacg cgaccatgcc
     3721 cttgctcgcg aagtcgcggg cgacctgctg gtaggtggac aggaggcgtg tcacctcctc
     3781 cttgcgcttc agccggtttg ccaggaaggc acgccgggtc accaggtcgt cccggcctcg
     3841 gcgggcggcc gtcaggtctt cctg
//
LOCUS       DATMZD010000271         4341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_18557,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000271 DATMZD010000000
VERSION     DATMZD010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4341
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18557"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="VN478_05595"
     CDS             complement(<1..382)
                     /locus_tag="VN478_05595"
                     /inference="COORDINATES: protein motif:HMM:NF022427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 family protein"
                     /protein_id="HWQ22180.1"
                     /translation="MQHELTAHMDLLNDKGELNARGWARQLIATYNRESIAAGWLRIK
                     EWDYYCVLAPDYGIAMTISDVGYLADISVTWLDFVKRTEATATIMKPFTRGSLNLPRT
                     SETGDIAYKDSQIDMRFTRNPASRV"
     gene            complement(413..1306)
                     /locus_tag="VN478_05600"
     CDS             complement(413..1306)
                     /locus_tag="VN478_05600"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013919087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWQ22181.1"
                     /translation="MEKRPFGKTGVELPILSLGCQRLVNEEGCDQDHAVAIIERALQR
                     GISYFDTAPAYSEGESERRVGLVAMSRRMDMWIATKVNETTRDGARRQLEASLGRLQT
                     DHVEEVRLHNVFSFDRLDAMTGKGGALHALTEARDEGLVRFISISGHANPQVLVEAIR
                     RFPFDTALCAVSALDHFIYSFAEEFLPFALQKCMGVVGMKVLGFKALAEDWKRALRYS
                     MALPVTTVITGCSTIEQLDGNIDVAESFVPMTSVERLAFFRDILPLVTPQNMPWKSKE
                     WARKEWIERKEPFGLTRSGCQ"
     gene            complement(1565..2419)
                     /locus_tag="VN478_05605"
     CDS             complement(1565..2419)
                     /locus_tag="VN478_05605"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HWQ22182.1"
                     /translation="MTKGTIAIITDSSCDIPRDYIEQHHVFSLPLQVNMPEGQFFDGV
                     DITPDEVYSRMPKVIPSTSQPSPGHVQQLLERIKQEGYREAIAICISSGLSGTYSVFC
                     MCAKEVEGLVVHVVDSHRLSMALGLLVMQAVEMNEEGRPCEEILASLNDGWKKTNAFF
                     CMPSLSYLIKGGRIGLVEGTIGTLLRIIPVISINNEEGRYYTYAKMRSYPLAIKKIED
                     TIRNLVKNRIVNIAVMHGGALEQAKKVYTSLATLEGLRSIYMSQISPALGVHTGPGLL
                     GIAYRVID"
     gene            2690..2992
                     /locus_tag="VN478_05610"
     CDS             2690..2992
                     /locus_tag="VN478_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HWQ22183.1"
                     /translation="MSTRTEEFKVSGEKLLTQVKDLVHQGNVRRITIKDKDGVTLFEV
                     PLTAGVVGAVLLPVWAAIGAVAALAASYTIAVELVDAPAKPTVEGSSVDRTDTPAQ"
     gene            3129..3428
                     /locus_tag="VN478_05615"
     CDS             3129..3428
                     /locus_tag="VN478_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22184.1"
                     /translation="MDDERVRLEGIVSALEVKISELDEDNVAVEEATRLRAELDEAYE
                     QIDDSSLTDDAIDDLMDRLDDLQDILEDLADGDTDWDDEEKEEEEADEAGDDEDD"
     gene            complement(3543..>4341)
                     /locus_tag="VN478_05620"
     CDS             complement(3543..>4341)
                     /locus_tag="VN478_05620"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HWQ22185.1"
                     /translation="YTTVSTLLPSTSGARPGLGMWREEVDTHTATLLIASCPGTYGFT
                     PWIDYKLNLAGVLSMKYDPGKAAYAIMRVRQLVPQAIAAGLRFKDVPAISWAFAAVVD
                     LSARGLVTGYEDGKFHPNNAVTRAEYAKLVCSALGVAPERVTSDPFKDVTSSYWAAGY
                     VAAAVRKGWLTGYPGSLFNPEEPIGMAQALAVIARSQGWRDTAALPYEDVLPGYWAHA
                     FIEACFARGIIRNPDPGIASGGKLNPEGDCTRAQACVLFSRLLAAEP"
BASE COUNT          867 a         1285 c         1314 g          875 t
ORIGIN      
        1 ggacgcgtga agcggggttg cgcgtgaagc gcatatcgat ctggctgtcc ttgtacgcga
       61 tgtcgcctgt ctcggacgtg cgcggcaggt tgagcgaccc gcgggtgaac ggcttcatga
      121 tggtcgccgt tgcctcggtc cgcttcacga agtccagcca ggtaacggag atgtcggcca
      181 ggtagccgac gtccgagatc gtcatggcga tgccatagtc cggggccagg acacagtagt
      241 agtcccactc cttgatgcgc agccaaccgg cggcgatgct ttcgcggttg taggtggcta
      301 tgagctgtcg cgcccaccct ctggcgttca gttcaccctt gtcgttcagc aggtccatgt
      361 gcgctgtcag ttcatgctgc atcatcatcc cccttttcgg tacggcttgt catcactggc
      421 agccgctgcg tgtcagtccg aatggttctt tccgttctat ccattccttg cgcgcccact
      481 ccttcgactt ccagggcatg ttctgcggtg tgacgagcgg caggatatcc cggaagaacg
      541 ccaggcgctc gacgctcgtc atcgggacga aactctcggc gacgtcgatg ttgccgtcca
      601 gctgttcgat cgtggaacaa cccgtgatga ccgtggtgac cggcaaggcc atgctatagc
      661 gcagagcccg cttccagtct tctgccagag ccttgaagcc caggaccttc atgccgacta
      721 cgcccatgca cttctgcagg gcgaacggca ggaactcctc tgcgaagctg tagatgaagt
      781 gatccagcgc tgagacggca cacagggccg tgtcgaacgg gaaccgccgg atcgcctcaa
      841 cgaggacctg gggattcgcg tgccccgaaa tactgatgaa ccgcaccaat ccttcgtccc
      901 ttgcctcagt aagcgcgtgg agcgcgccac ccttgccggt catggcgtca agccgatcga
      961 agctgaagac attgtgcaga cgtacttctt ccacatggtc ggtctgaagt cggccgaggc
     1021 tggcctcaag ctgtcggcgt gcaccgtccc gcgtggtctc attgaccttg gtggctatcc
     1081 acatgtccat gcgacggctc attgccacaa gccccacgcg tcgctcgctc tcgccctccg
     1141 agtaggcagg cgcggtgtcg aagtacgaga tgccacgctg cagcgcccgt tcaatgatgg
     1201 cgacggcatg gtcctggtcg caaccttcct cattcaccag tcgctggcat ccgaggctca
     1261 ggatcgggag ttcgacaccg gtcttgccaa atggtcgctt ctccatgagg ttcctccctt
     1321 ccgcggcggg gccgcgtcca ccaatagtat agcctcacgg gccagccgta ccagcgcgct
     1381 cgaaaccccg gatccaggaa acgaaacaag gcgacagcct gacaaacccg gcgcagcgac
     1441 tgcgctggtg cggaccacga tcagaggaga tgggacgaac cgacgggtgc cggccccgct
     1501 cgaaagcggc gggtccgact acgtctgcat ggcttccggc tcctgacgca ggaccgcggg
     1561 tggctcagtc gatgacccga taggcaatgc ccagcaggcc tggaccagtg tgaactccca
     1621 gagcaggact gatttgactc atgtagatgc tgcgcagacc ctccaaggtt gccagagagg
     1681 tatagacctt cttggcctgt tccagggctc cgccgtgcat gacagcgata ttgacgatcc
     1741 tgttcttcac caggttcctg atggtatcct ctatcttctt gatggcgagg ggatagctgc
     1801 gcatcttcgc gtacgtgtag tagcgacctt cttcgttgtt tatcgagatg accggtatga
     1861 tcctcagcag cgtacctata gtgccctcca ccagaccgat tctgccgccc ttgatgaggt
     1921 agctgagaga aggcatgcag aagaacgcat tggtcttctt ccagccgtca ttcaggctgg
     1981 cgaggatctc ttcgcaggga cgaccttcct cgttcatctc gacagcttgc atcacgagaa
     2041 gccccagggc catggagagg cggtggctgt cgacaacatg cacgaccaat ccctcgactt
     2101 ccttcgcaca catgcagaag acactgtatg ttccgctcag gcccgacgag atgcatatgg
     2161 cgatggcttc cctgtagccc tcttgcttga ttcgctcaag cagctgctgg acgtggcccg
     2221 gggagggttg agaggtggag ggtatcacct ttggcatacg agagtatacc tcatcaggcg
     2281 tgatgtccac gccgtcaaag aactgtcctt ccggcatgtt gacttgaaga ggcagagaga
     2341 agacatgatg ctgttcaatg tagtctcttg gtatatcaca gctgctgtcg gtaataatgg
     2401 ctatcgtgcc ctttgtcatc ttgtcactcc ttggaatagc tgtcgtgtct ttgagaccct
     2461 ggcacaaagc agttgccgca ccaggagtct caggattata ctgtgtcaca gtctgcccaa
     2521 ccagatgtgg caagttcagc atctgggttt atggtatcgc caattcctcg aaatggcaac
     2581 ccgatcgcca tgtaacctcg actccttctc ctgcgtagac gaagtgacga caggacaggc
     2641 acgacgcagt gatcctgcga atcgacgaca agaccaaagg agaagagaca tgagcactcg
     2701 aacggaagag ttcaaggtca gcggcgagaa gctgctgacg caggtgaagg acctggttca
     2761 tcagggcaac gtgcgacgga tcaccatcaa ggacaaggat ggggtcaccc tctttgaagt
     2821 cccgcttacg gcaggtgtcg taggggcggt tctgcttccc gtgtgggcgg caatcggggc
     2881 tgtcgccgct ctggcagcat cctacacgat cgctgtcgag ctggtcgacg ctccggcgaa
     2941 gccgactgtc gagggctcgt ccgtggacag gacggacacc cctgcacagt agctctcgct
     3001 cgcagaggca cttcagacgg ccgggtcttc gcccggccgt ctggcatttc cgttcatgag
     3061 gcttgaaacg cgtggcgttt tccctatagt gccggcatca cacccacatg tttgcatggg
     3121 aggcagtcat ggatgacgag agagtacggc tcgagggaat cgtcagcgcg ctcgaagtga
     3181 agatcagcga gcttgacgag gacaatgtcg ctgttgaaga ggcgacacgc ctgcgtgcag
     3241 agcttgatga ggcatatgaa cagatcgacg actcttccct gacggacgac gccatcgacg
     3301 atctgatgga ccggctggac gacctgcagg acatccttga ggaccttgcc gacggcgaca
     3361 ccgactggga tgatgaggag aaggaagagg aagaggcaga cgaagctgga gacgacgaag
     3421 acgactaggc atctgccaag gccttctgaa tggacgacgc cccggagaaa tccggggcgt
     3481 ttgtgtcgag aacgatcact gtctctccgt catcggagct gtctatgcga cggacttgat
     3541 ggttacggct ctgcggcaag caggcgactg aagaggacac acgcctgcgc gcgcgtacag
     3601 tccccctcgg ggttcagctt cccgcccgat gcgatgccgg gatctggatt cctgatgatg
     3661 cccctcgcga agcaagcttc aatgaaggca tgcgcccagt agccgggcag gacgtcctca
     3721 taggggagcg ccgccgtgtc tctccagcct tgtgaacgcg caatgacagc aagggcctgg
     3781 gccatgccta tgggctcctc cgggttgaag aggcttccgg gatacccggt cagccagccc
     3841 ttgcggacag ccgccgcaac atagcctgcg gcccagtagg aggatgtcac atccttgaag
     3901 gggtctgacg tcacgcgctc cggcgcgact ccaagggcgc tgcagacaag cttggcgtat
     3961 tcggctcgcg tgactgcatt gttcggatgg aacttgccat cctcatatcc agtgaccaga
     4021 ccgcgtgcgc tcaggtcgac gacagccgcg aaggcccacg agatcgcagg gacgtccttg
     4081 aaccggagtc cggcggcaat ggcctgaggt accagctgcc gtacccgcat gatcgcgtat
     4141 gcggccttgc cgggatcgta cttcatcgac aagacgcccg cgagattcag cttgtagtcg
     4201 atccagggag tgaagccgta tgtgcccggg caggaggcga tcaggagtgt cgccgtgtgt
     4261 gtgtcgacct cctcccgcca catgcccagc ccaggacgtg cgccgctggt cgacgggagc
     4321 aatgtcgaaa ccgtcgtgta c
//
LOCUS       DATMZD010000272         2136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_58468,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000272 DATMZD010000000
VERSION     DATMZD010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58468"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="VN478_05625"
     CDS             <1..586
                     /locus_tag="VN478_05625"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWQ22186.1"
                     /translation="LTTAIKPMAIPGLSRIPVLGPILFSQDLVVYVGYILVALFWFLL
                     FKTKAGVNLRAVGENAKAADAMGINVAGTRYFWTILGGALAGAGGAYITCCIHPYWLN
                     GITSGKGWIAVALVVFAMWNPFNALVGAFLFGSIEAMQLQLQAGGTYINSALLSMLPY
                     VTTFVVIIVATLIVRVRHVGTPRELGTPYAREEK"
     gene            complement(672..1049)
                     /locus_tag="VN478_05630"
     CDS             complement(672..1049)
                     /locus_tag="VN478_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22187.1"
                     /translation="MARSRRSIVIAAAVLVIAGGVLAALLFVPSLSSWRGSRVRCSLQ
                     LDRPAPLPQHTPLVLTFVTRGILGPYAYEVSVDGDVVGQGTTSTDRFTVPATFGASGV
                     SMISVTLTVDDKPLDFLFTLAVS"
     gene            complement(1065..2057)
                     /locus_tag="VN478_05635"
     CDS             complement(1065..2057)
                     /locus_tag="VN478_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HWQ22188.1"
                     /translation="MNELIRTLTQTWGPTSYEEGIREVITHELGCLHVDSRVDALGNL
                     IYCIPAPGKPRLLLSAHMDQIGIIITHVDKDGFLRFGAVGGLDPYKVLGQRFVFANGT
                     VGIAAVESKEHKTLESLTLDKLFLDIGAASREAALLEVAVGDFAVYAGGCVQVGNRVT
                     SAALDDRIGCYVLIEALKVVAGKNLNYETYAMFSVQEEIGIKGAKVGAWGVQPDLAIA
                     LDVTAWGDTPESYVLDIGLGKGPAIKVKDAGMVATPAVRDLLIETAKKHGIPFQLEVL
                     QGGTTDAAAIQINAGGVPTGAISIPDRYVHSASEMVDLGDVEGCINLLSALLLK"
BASE COUNT          406 a          645 c          650 g          435 t
ORIGIN      
        1 tctcacgact gccatcaagc caatggctat cccagggctg tccaggatcc cggtcctggg
       61 tccaatcctc ttcagtcagg atctggttgt ctatgtcggc tacatcctgg tggcgctgtt
      121 ctggttcctg ctgttcaaga cgaaggcggg agtcaacctc agggccgtcg gcgaaaacgc
      181 caaggccgca gatgccatgg gcatcaatgt cgcaggcacg cgctatttct ggaccatcct
      241 gggaggggcg cttgcaggtg cgggaggtgc gtacatcaca tgctgcatcc acccgtactg
      301 gctcaatggc atcacgtcgg gcaaggggtg gattgccgtc gctctcgtgg tctttgccat
      361 gtggaacccc ttcaacgctc ttgtcggcgc gtttctgttc ggaagcatcg aggccatgca
      421 gcttcagctg caggcggggg gcacatacat caacagcgca ctgctgtcga tgctgcccta
      481 cgtcacgacg tttgtcgtca tcatcgtggc gaccctcatc gtacgggtgc gccacgtagg
      541 cacgcccagg gaactgggca cgccatatgc acgcgaggag aagtaggcgc tgctactgag
      601 tcagagagat ttggactctt gcgaaccggc agttgtcgtt gctgtcgatt tcgtctgttg
      661 cggaccgcct gtcaggatac tgccagtgtg aacaggaagt ccaggggctt gtcgtccaca
      721 gtcagtgtga ccgagatcat cgatacgcct gaggccccga acgttgctgg aacagtgaac
      781 cggtcggtgg atgtcgtccc ctgaccgacg acatcaccgt cgaccgagac ctcgtaggcg
      841 tatgggccga ggatgccccg cgtcacgaat gtcaacacga gtggcgtgtg ctgtggcagc
      901 ggagctggac ggtccagttg cagggagcaa cggacgcgcg accctctcca gctcgaaaga
      961 gagggaacga acaggagtgc tgcgagcacg ccgccggcaa tcacgaggac tgccgcggcg
     1021 atgacgatgg accttcgaga acgtgccacg gggacacccg gccgctactt cagaaggagg
     1081 gcggacagca ggttgatgca gccttcgacg tcgccaaggt ccaccatctc gctggcgcta
     1141 tggacatacc ggtctggaat gctgatcgct ccagtaggaa cgccgcctgc gttgatctgg
     1201 atggcggctg catcggtcgt gcctccctgc aggacttcca gctggaacgg gatgccatgc
     1261 ttctttgcag tttcgatcaa caggtcgcga acggcgggcg tcgcgaccat gcctgcatcc
     1321 ttcaccttga tagccggccc tttgcccagg ccgatgtcca ggacgtagct ctccggagta
     1381 tcgccccagg cggtcacgtc cagcgcgatg gcaaggtccg gctggacgcc ccaggcgccg
     1441 accttggcgc ccttgatgcc gatctcttcc tgcacgctga acatggcata cgtctcatag
     1501 ttcaggttct tccctgcgac gaccttgaga gcctcgatga gcacgtaaca gccgatgcgg
     1561 tcgtcaagag ccgcggatgt cacgcggttt ccgacctgca cgcagccgcc cgcatatact
     1621 gcgaaatcgc cgacggcaac ctccagcagc gcagcttccc ttgatgccgc gccgatgtcc
     1681 aggaacagct tgtcgagcgt caggctctca agcgtcttgt gttccttgct ttcgacagcc
     1741 gcgatgccga ccgttccatt ggcaaacacg aaccgctgcc ccaaaacctt gtacgggtcc
     1801 agcccaccga ctgcgccaaa gcgcagaaag ccgtccttgt cgacgtgggt gatgatgatg
     1861 ccgatctggt ccatgtgggc ggacagcagc agccttggct ttcctggagc gggaatgcag
     1921 tagatcaggt ttcccagagc atccaccctg ctgtccacat gcagacaacc gagttcatgc
     1981 gtgatgacct cgcgaatacc ctcttcatag ctggtgggtc cccaggtttg cgtcaaagtg
     2041 cggatgagtt cgttcatcta tgcctccgtc gtggcgagtt gtgccaggaa cttcgaaagg
     2101 agcgacagcg tggtgtcgta gtcgcacatc ttgatg
//
LOCUS       DATMZD010000273         4750 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_18584,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000273 DATMZD010000000
VERSION     DATMZD010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4750
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18584"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="VN478_05640"
     CDS             <1..603
                     /locus_tag="VN478_05640"
                     /inference="COORDINATES: protein motif:HMM:NF020130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HWQ22189.1"
                     /translation="WFMSHPEFRYALLIGEYGMTKYVDKDDKYTASLFADGAAAMIIE
                     NTGDSIIASKFIADGTYHDYLGVFGGGTRYPINDDTLAKGLHKLRILNKYDQNPNLVA
                     WPGLVTSLMETVGEPLSAIDLILFTQINLAVIKKVLETLGIPESRSHWIMDRYGYTGS
                     ACIPMALYDARKQGKAKPGDLVVLVASGAGQAMGAVALRL"
     gene            627..1616
                     /locus_tag="VN478_05645"
     CDS             627..1616
                     /locus_tag="VN478_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HWQ22190.1"
                     /translation="MRTVRIAGTGIYTPTRSMPTEEVGTMLGVDLSQQIQTGGIEVKK
                     IMADDEEASDLAVYAGMQALRNAGVKPSELDLIIVSTDTPDYISPCTASIVQHRLLAK
                     NAGLFDTNCACAGWVTALNTATKYIMADRHFHKALVIGTYAMTRFLNWHDKVTAPTFS
                     DGAGAAVLVEEESETGGYLGSMLYADGYYWDYLGVYLGSARLPTAESVANQEHKVQYN
                     QYKRFPDVNSSHWPRLVRETLAQVNLTPQDADMLLFTQVRRNTIEGVMNTLGLPMSRT
                     HTVMEKFGYTGSACVPMALHDALEQDLIHSGDIVVMTASGGGYAMACYAMRWL"
     gene            1626..2384
                     /locus_tag="VN478_05650"
     CDS             1626..2384
                     /locus_tag="VN478_05650"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWQ22191.1"
                     /translation="MAYALFEGRRVYSERLGEGSKTVIFGHGNLASANSWRLMMPFLP
                     LDRKNVFVDFPGFGKSDNIAPTTVEHCADALIAWQRHWHLEPAVYVGHSMGAVIGLMA
                     ALKGAQFSKLVLIGCGPADGYPMTEQMRASFLMLTQNIPLIEAMTRKNNEPFGLAEDI
                     LKEIIADAVRCVPQGYTEMADSLAGVRMSAQLGTLSMPITFLHGDRDSVVPIDWIRPT
                     VAAAHGKLVVLEGQVHLPMLVEPERFAATLEKEL"
     gene            2391..3758
                     /locus_tag="VN478_05655"
     CDS             2391..3758
                     /locus_tag="VN478_05655"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWQ22192.1"
                     /translation="MGERRRQTALATSSAVAVLPTPSTSERIVSSALKLFFTRGYSQT
                     SVAMIARDVGISNSAFYRHFKSKEGALLAVLETFSEDFTGALSIGMARSEGLEGKIRA
                     FYSTILEVVGRDTHLYRVFTETEFNGYKFVRDFYSRVVAIVHDQLSGLVPAELDLDDV
                     GYYLLGSVYLVAVKKTLWENLFTVDGFCTQLVDFALYGIDARGDAPEDLVPLPEAAPL
                     QKPEVRLSKGERTQKNLLTAAEHIFGEQGYWNADIYTIARAAGVAPGTTYLYFDSKLE
                     LLSRLVVEVNEGLRRHTAQALRGLSDRRIVEVAALESFCNYIREHKDSYRVVREAEFV
                     DKDLGRWYYTRLSDPYTVVLERAMVAGEIRPGNPEITALALMGIGHWLGLRWVLWSDH
                     LDAEVSPTALTSSVRLVLHGLAGAVLRAASKAENEHHKGVESTGRRKRTKVADRMAAS
                     DGQND"
     gene            3799..>4750
                     /locus_tag="VN478_05660"
     CDS             3799..>4750
                     /locus_tag="VN478_05660"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HWQ22193.1"
                     /translation="MKKVLSLVLVIALVVGLFAGVRPANAATKDFFMVLQVGFKQYAL
                     NGLPLNSEVAPEIVGGRTFVPVRLIAETYGAAVTWNGTTRTVSVELGETEVRLTIGVK
                     TALINYEEKALEAAPYIKAGRTMVPLRLISEAFGLSVYYDAQTKSISIKSQTTGSVPG
                     ITGNQILIGSFSVQTGPYAVVGIPFSHGMQAYFNMVNEAGGVYGRKINLKVADDGFNP
                     ANTVTIVKKFVTEDKVFAVVGGLGTPGVLAVMDYLNEQKVPFVYQASGVSKIAIPAKN
                     YVFAVQPNYINEGQIFAKYVASLGLKNIAVLYSNDDTGNEG"
BASE COUNT          999 a         1327 c         1450 g          974 t
ORIGIN      
        1 tggttcatgt ctcacccgga gttcaggtac gctctgttga ttggagaata cggcatgacc
       61 aaatacgtgg acaaggacga caagtacacc gcatctctct tcgccgatgg agctgcggcc
      121 atgatcatcg agaacaccgg cgacagcatc atcgcgtcga agttcatcgc cgacggcacc
      181 taccacgact atctgggcgt attcgggggt ggcaccaggt atcccatcaa cgacgacacg
      241 ctagccaaag ggctgcacaa gctgcgcatc ctcaataagt acgatcagaa tccgaacctt
      301 gttgcctggc cggggctggt tacgagcctg atggagacgg tcggtgaacc gttgagcgcg
      361 atcgacctca tcctgttcac gcagatcaac ctcgccgtca tcaagaaggt actggagacc
      421 cttggcatcc cggagtcaag atcgcactgg atcatggaca ggtacggcta caccggctca
      481 gcctgcattc ccatggcact gtatgatgct cgcaagcagg gcaaggcgaa acccggagac
      541 ctggtcgtcc tggtggcatc gggagcagga caggcgatgg gcgcagtagc gctgcgcctg
      601 tagccgactg gcgagggagt ggtcagatga gaacagtgcg tatcgcgggg accggcatct
      661 acacgccgac gcgttccatg ccgaccgaag aggttggcac gatgctcggg gtcgacctgt
      721 cccagcagat ccagacgggt ggcatcgagg tcaagaagat catggcggac gatgaagaag
      781 cttccgatct tgcggtctat gcgggaatgc aggctctcag gaacgcgggc gtcaaaccat
      841 ccgagctgga cctgatcatc gtaagcacag atacgccgga ctacatctca ccgtgcacag
      901 catcgatcgt ccagcatcgg cttctggcca agaacgccgg actgttcgac accaactgcg
      961 catgtgcggg ttgggtcact gcgctgaata cggccacgaa gtacatcatg gcagaccggc
     1021 atttccacaa ggctcttgtc atcggcacct atgccatgac gcgattcctc aactggcatg
     1081 acaaggtcac cgctcctacg ttctcggatg gcgccggcgc cgccgtcctc gtcgaagagg
     1141 aatcggagac aggcgggtat ctgggatcga tgctctacgc tgacggatac tactgggact
     1201 acctgggcgt ctacctgggg tcggccaggc tgccgactgc cgagtccgtg gcaaaccagg
     1261 aacacaaggt ccagtacaat cagtacaaga gatttcccga cgtcaactcg tcgcattggc
     1321 cccggctggt ccgggagacg ctggcacaag tcaatctgac gcctcaggac gcagacatgc
     1381 ttctgttcac ccaggtccgc aggaacacga tcgaaggagt catgaatacg ctgggcctgc
     1441 cgatgtccag gacgcacacc gtcatggaga agttcggcta cacgggttcg gcctgtgtcc
     1501 cgatggcttt gcatgacgcc ctcgagcaag acctcatcca cagcggcgac attgtcgtca
     1561 tgacggcttc aggcggcggc tacgcgatgg cgtgctacgc catgcgctgg ctctaggggg
     1621 tcgagatggc atacgcattg tttgagggaa ggcgagtcta ctccgagaga cttggagaag
     1681 gcagcaagac tgtgatcttc ggacatggga acttggcctc tgccaactcg tggcgtctga
     1741 tgatgccatt cctcccactg gaccggaaga acgtgtttgt ggacttccct ggtttcggca
     1801 agtcggacaa cattgcgccc acgacagtcg agcactgcgc agacgcattg atcgcatggc
     1861 agagacactg gcatcttgag cctgccgtct acgtgggtca ctccatgggt gccgtcatcg
     1921 ggttgatggc ggcgctcaag ggagcgcagt tctcgaagct ggtgctgatc ggctgcggtc
     1981 ctgcggacgg ctaccccatg acggaacaga tgcgagcctc gtttctgatg ctcacacaga
     2041 acattcctct catcgaggcc atgacgcgca agaacaatga accatttggc cttgcagagg
     2101 atattctcaa ggagatcatc gctgacgctg tccgctgcgt gcctcagggc tacacggaaa
     2161 tggcagattc actggcgggc gtacggatgt ctgcacagct cggcaccctg tccatgccga
     2221 tcacgttcct ccatggcgac cgggacagtg tggtgccgat cgactggatc cggccgacag
     2281 tggcggcggc tcatgggaag ctcgtcgttc ttgaaggtca ggtacatctc ccgatgctcg
     2341 tcgaaccgga gcggttcgcg gcgacccttg agaaggagct gtaggaagtg atgggtgaac
     2401 gtcgccggca gaccgcgcta gccacgtcgt cggcagttgc tgtcttgccg acaccttcga
     2461 cgtcggagcg gatcgtcagc tcggcgctga aactgttctt cactcgtggc tactcacaga
     2521 cgtctgtggc gatgatcgct cgtgatgtag gcatctcaaa ctcggcattc tacagacact
     2581 tcaagtccaa ggaaggcgcc ctgctggctg ttctcgagac gttctcggag gacttcaccg
     2641 gggcactgtc gatcgggatg gcacgatcag agggtctgga gggcaagatc agggccttct
     2701 actcgacgat tctggaggtg gtcgggcgcg atacacattt gtacagggtc ttcacggaaa
     2761 ctgagttcaa cggatacaag tttgtgcggg atttctactc ccgggtcgtg gctatcgtgc
     2821 atgaccagct cagtgggctg gtccctgccg agctggatct ggacgacgtg ggctactacc
     2881 ttctcggatc ggtgtacctg gtggcggtca agaagacgct atgggagaac ctcttcacgg
     2941 tcgatgggtt ctgcacgcag ttggtggact tcgcgctgta tggcatagac gcgcgcggcg
     3001 atgctcccga ggacctcgtg ccacttcccg aggctgctcc tctgcagaag cccgaggtgc
     3061 gtctctcgaa aggggagcgt acccagaaga acctgctgac ggctgcagaa cacattttcg
     3121 gtgagcaggg ttactggaat gctgatatat acaccatcgc ccgagctgca ggggttgcac
     3181 ccgggacgac gtacctgtac ttcgactcca aactggagct gctctcacgg ctggtcgtag
     3241 aagtcaatga gggcctccgg cgccacacgg cccaggcact gcgagggctg tctgacaggc
     3301 gcatcgtcga ggtggcagcc ctggagtctt tctgcaacta catacgtgaa cacaaggact
     3361 cctaccgcgt cgttcgtgag gcggagttcg tcgacaagga ccttggcaga tggtactaca
     3421 cgcgtctgtc agacccctat acggttgtgt tggaacgagc catggttgcc ggtgagattc
     3481 ggcccggcaa ccctgagatc actgcgctgg ctctcatggg cataggtcac tggctgggtt
     3541 tgcggtgggt cctgtggagc gatcatcttg atgccgaggt gtcgccgacg gcactcacga
     3601 gttccgtacg actcgtcttg cacggacttg caggagccgt ccttcgggcg gcttcgaaag
     3661 ccgagaacga gcatcacaag ggggtggaat cgactggcag acgcaagcgg acaaaggtgg
     3721 ccgacagaat ggccgcatcc gatggacaga acgactgaat gtgttgcaac taattcttgc
     3781 agaacaggag gcatgacaat gaagaaggta ctgtcactgg tgctggtgat tgccctcgtc
     3841 gtcgggttgt ttgcgggcgt gcggccggcc aacgcggcaa ccaaggattt cttcatggtg
     3901 ctgcaggtcg gtttcaagca atatgcgctt aacggacttc ccctcaattc ggaagttgct
     3961 cctgagattg tcggcggtcg gacatttgtt cctgttcgcc tgatagccga aacgtacggt
     4021 gccgcggtga cctggaacgg cacaacgcgg accgtttctg tcgagttggg tgagaccgaa
     4081 gtcagactca cgatcggagt gaagacggct ctgatcaact acgaagagaa ggctctggag
     4141 gcggcaccgt atatcaaggc gggccgtacc atggtacctc tgcgtctgat cagcgaggcc
     4201 tttggcctgt cagtatacta tgatgcccag acgaagagca tctccatcaa gtctcagacg
     4261 accggttcgg tccctggaat cactgggaat cagatcctca tcggctcgtt ctcagtgcag
     4321 acgggtccct atgccgtagt cggcattccg ttctcacatg gtatgcaggc gtatttcaac
     4381 atggtcaatg aggcaggcgg ggtctacggt cgcaagatca acctgaaggt cgcagacgac
     4441 gggttcaatc ctgcaaatac cgtgaccatc gtcaagaagt ttgtcactga ggacaaggtg
     4501 ttcgccgtgg tcggcggcct tggtactccc ggcgtcctcg ccgtcatgga ctacctgaac
     4561 gagcagaagg tcccgtttgt ctatcaggcg tccggcgtct ccaagatagc gattcccgcc
     4621 aagaactacg tgtttgccgt tcagcccaac tacatcaatg agggccagat cttcgcgaaa
     4681 tatgttgcca gcctgggtct caagaacatt gccgtgctct acagcaatga tgataccggc
     4741 aacgaagggt
//
LOCUS       DATMZD010000274         5573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_18619,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000274 DATMZD010000000
VERSION     DATMZD010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5573
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18619"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="VN478_05665"
     CDS             <1..537
                     /locus_tag="VN478_05665"
                     /inference="COORDINATES: protein motif:HMM:NF022059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HWQ22194.1"
                     /translation="NRGIVEAPFGVTLRELIYDFGGGVKGGKRIGFIQTGGSAGTVLS
                     DKYLDIPLDFASMKAFNVSIGSGVILVASDEVPLLPFLAEVAHFFWHESCGKCTPCRE
                     GTLQIKAIMDSLVNRTADPASLQRLGSLLSLLTDTSFCGLGQAAPLAFESSLRNFRGE
                     FETALAESHVPDVCQEEA"
     gene            569..907
                     /locus_tag="VN478_05670"
     CDS             569..907
                     /locus_tag="VN478_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22195.1"
                     /translation="MKLTEVIEITGATCIMGEHLDSIEVNHAFASDLMSDVLAMSHPD
                     TLLITGLTTPQIIRTAQMLDLPAILVARGKDVPQDVADLARTTGIVLLATHDIMYVAS
                     GKLYVRGLTG"
     gene            911..1840
                     /locus_tag="VN478_05675"
     CDS             911..1840
                     /locus_tag="VN478_05675"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWQ22196.1"
                     /translation="MSLEQSVEALRYLGLFSNLDASSVMSTKVRALHPEDRVAAARII
                     MRENSISGVPIVDVDFRLVGLISVSKIIEALADGRLADSVDSVMTHDVVSLHVHDSFE
                     TILSCFQRFKFGRFPVVEDDGKLVGMITDTDVLSAIIRSFYSVYVHDQRRTITLGSFS
                     ALSKEFENSADFAHDISCTGLENAGEGSARLKAFLQTKGFSESVVRRASVCAYEAEIN
                     VCIHAGGKGSLRAIAHDGEIIVRVEDEGPGIENIDLAMKEGWSTATDAIRELGFGAGM
                     GLPNIRRFSDHLVIISGKDKPTKVEMIFLADKE"
     gene            1849..2181
                     /locus_tag="VN478_05680"
     CDS             1849..2181
                     /locus_tag="VN478_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur binding hydrogenase"
                     /protein_id="HWQ22197.1"
                     /translation="MTVHDVIESLGADVAVEGDTTRQVTSGYTCDLLSEVIAKAIPGS
                     AWATIQTHVNVIAVAAMVGIAVVIICEGRTCEADVVEHATKEHLTVLYSRDSAYVVSG
                     KLYVLGLR"
     gene            2185..2916
                     /locus_tag="VN478_05685"
     CDS             2185..2916
                     /locus_tag="VN478_05685"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HWQ22198.1"
                     /translation="MLDVPGDLHLHSCLSPCADITMLPGEMAGRLAARGIRVASLTDH
                     NAACNVPAFQRAFRRDDILLVPGMEATTAEEVHLLCYFETMDALWTFGRRLLASYPRV
                     VNDPERFGYQLVCNERDEYVGQFPFLLSMASLLSLEELRALAEQLDGVVVPSHLDRRY
                     GLLYQLGIVTSDMEFPTYEVAHKINIEKVASQLPGNPQFVSNSDAHNLDSISPPRCVF
                     EIEELSVHEVLMALRHESGRGVRLL"
     gene            2913..3494
                     /locus_tag="VN478_05690"
     CDS             2913..3494
                     /locus_tag="VN478_05690"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWQ22199.1"
                     /translation="MRTIADHVLDIASNSVTAGAGDIWLDIDEDPAKHLLTFTVKDNG
                     RGMDADIRSRVFDPFFTTRSHAIRRFGLGLPFLKENTELTGGGVDLESIPDEGTTLRA
                     TFHTDHIDCLPFGDLASSIFALLLSSDRVRWHIHRARGDADYAIGTEELEQVFSPEEL
                     SDPQSQSLLLEFLRGQELSVAESDGREFLEGGV"
     gene            3497..4198
                     /locus_tag="VN478_05695"
     CDS             3497..4198
                     /locus_tag="VN478_05695"
                     /inference="COORDINATES: protein motif:HMM:NF003995.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HWQ22200.1"
                     /translation="MSTTTKYGFEDISKATISRMATYLRCLTYLEQQGTATVSSRELA
                     SLTGVSPEQVRQDLSVFGRFGKRGTGYDIVRLKTEVEVIFGRGVEQWKCCIVGVGSLG
                     SSLVASHMVQKWGFRYVAAFDRDPNKVGTRVERIPVYPVDDLKAVVRREHIAIGVIAV
                     PASSARAVARTLCDAGIRGILNFAPQSIKVPEKIPVVSVDISQEFLKLSFYIQSAEQR
                     AREEQSQIEAASHSE"
     gene            complement(4208..4942)
                     /gene="fdhD"
                     /locus_tag="VN478_05700"
     CDS             complement(4208..4942)
                     /gene="fdhD"
                     /locus_tag="VN478_05700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HWQ22201.1"
                     /translation="MLTSIWRYDPSGNVVQCQDEVAEERPLTIYVNGTLAVRLASSGD
                     LMEAAAVGHAVSEGWARPAQILVVTVEGRSAFLTLSRDAPGPDSPMSTDIDCISGDAL
                     AEDIAVSRGFTVSARRLRDLAEEMQRSAVQWRTTGGVHAALVAQGDNVFCCEDISRHT
                     ALDIVIGYAVAHSWNLGEAVLMGTGRIPTQAVAQAARAGIPVLASRSATTAQAVALAE
                     RIGSTLIAFVRGGRMNVYSHPERVVS"
     gene            5095..>5573
                     /locus_tag="VN478_05705"
     CDS             5095..>5573
                     /locus_tag="VN478_05705"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HWQ22202.1"
                     /translation="MGRIAEYYRAASLEDAFAKAKQSGSAAAYVGGGVELVLRRNPEV
                     TTLIDLSRCGLDYIIAAGEGIEIGSQTTLTTISRSADIRHYAGGSLAWWAGHIAHNNL
                     RDVITVGGAIGRNQPWNDVVPHLIALGAQVRLFDGVEQLVPLAGYIEARQPGVIIKGV
                     "
BASE COUNT         1115 a         1577 c         1695 g         1186 t
ORIGIN      
        1 aaccggggca tcgttgaggc tcccttcgga gtgactctga gagagctcat ctatgatttc
       61 ggcggcggtg taaagggagg caagcgcatc gggttcatcc agactggtgg cagcgccggc
      121 acggtcctgt cagacaagta cctggacatt ccgctcgact tcgcgtccat gaaggcattc
      181 aatgtctcga tcgggtccgg cgtcattctc gtcgcttccg acgaagtgcc gctccttccg
      241 ttccttgcgg aagttgcaca tttcttctgg cacgagtcct gcggcaagtg cactccgtgc
      301 cgtgagggga ctctgcagat aaaggcgatc atggattcgc tggtcaacag gactgcggac
      361 cctgcttccc tgcaaaggct cggcagtctg ttgagcctgc tgacggacac ttcgttctgt
      421 gggctgggac aggcggctcc gctggctttt gagtcatcac tgcgcaattt cagaggcgag
      481 ttcgagacag cactcgccga gtcacacgtt cctgacgtct gtcaggaaga ggcatagaag
      541 aataacgttc tggagaggag tagccaactt gaagctaacc gaagtcattg agattaccgg
      601 tgcaacgtgc attatgggtg aacacctgga cagtattgag gtgaaccacg cgtttgcctc
      661 ggacctcatg agtgatgtcc ttgccatgag tcaccctgac acactgctga ttaccgggtt
      721 gactactcct cagatcatca gaacggctca gatgcttgat ctacctgcta ttctcgtagc
      781 gcgaggaaag gacgtgcccc aggatgtcgc cgacctggca cgcacgacag gcatcgtcct
      841 tctggcgacg cacgacatca tgtacgtggc gtcgggcaag ctctacgtcc gaggccttac
      901 gggatagtag atgtccctcg agcagagcgt agaggcgctg cggtatctgg gtctgttcag
      961 caaccttgac gcctccagcg tcatgtctac caaggtacgt gcactccatc ctgaggatcg
     1021 tgtcgctgca gcgcgcatca tcatgcggga gaactccatt tctggtgtgc ccatcgtcga
     1081 tgtcgacttt cggctggtgg ggctcatctc agtctcaaag atcattgagg cgcttgccga
     1141 cgggcgtttg gcagactctg tcgactcggt gatgacacat gacgtcgtct ctcttcatgt
     1201 gcatgacagc tttgagacga tcctgtcttg cttccagcgt ttcaagtttg ggcgctttcc
     1261 cgtggtggag gacgatggaa agctggtcgg catgatcaca gacaccgacg tcttgtcggc
     1321 gatcattcgc agcttctact cggtgtatgt ccacgaccag cgacgtacga tcacactcgg
     1381 cagcttctcg gctctctcga aggagttcga aaacagtgcg gattttgcgc acgacatcag
     1441 ctgcaccgga ctcgagaacg ccggcgaagg ctcagcccgc ctcaaggcgt tcctccagac
     1501 caagggattc agcgaatccg ttgttcgacg ggcaagtgtc tgtgcttacg aggcagagat
     1561 aaacgtctgt attcatgcag gaggaaaggg ttcgctgcgg gcgatcgctc acgatggaga
     1621 gatcattgtg cgcgtggaag acgagggacc cggaatagag aacatcgatc ttgcgatgaa
     1681 ggaaggctgg tctactgcga ccgacgcgat cagagaactg ggctttggtg cgggcatggg
     1741 tctcccgaat attcgaagat tctctgatca ccttgtgatc atctcgggca aggacaagcc
     1801 taccaaggtt gagatgattt tcctggctga caaggaatag gaggcaacgt gacagtccat
     1861 gacgtcattg agtcactggg tgcggatgtc gcggtcgaag gcgacacgac tcgacaggtc
     1921 acatcggggt atacctgtga tctgctctcg gaagtaatag ccaaggcgat ccctggatca
     1981 gcctgggcga ctatccagac ccatgtcaac gtcatcgccg ttgcggcgat ggtcggcatc
     2041 gctgtcgtga tcatttgtga gggcaggact tgcgaggctg acgtagtcga gcacgcgacg
     2101 aaggaacatc tgactgtcct gtattccagg gactcggctt acgttgtctc gggaaagctc
     2161 tatgttcttg gactgaggta gctcgtgctg gatgtacccg gtgacctgca cctgcattca
     2221 tgtctgtcac cgtgcgcgga catcaccatg ttgcccggcg aaatggccgg acggctcgca
     2281 gcccgcggaa tacgggtggc ctctctgacc gaccacaacg ctgcatgcaa cgtgcctgca
     2341 tttcagcgag catttcggcg ggacgacatc ctgcttgttc cgggaatgga ggcaaccacg
     2401 gccgaggaag tccacctgct ctgctatttc gagacgatgg atgcactctg gacattcggt
     2461 cgcaggctgc tggcatcgta cccgcgcgtc gtcaacgatc ccgaacgctt cggctaccag
     2521 ctcgtctgca atgagcggga cgagtatgtg ggccagtttc ccttccttct ttcaatggcc
     2581 agcctgctga gtctcgaaga gctgagagcg cttgctgaac agctggacgg cgttgtcgtg
     2641 cccagtcacc tggatcgccg gtacgggctt ctctatcaac tcgggatcgt gacgtcggac
     2701 atggagtttc ccacctatga ggttgcgcac aagatcaaca ttgagaaggt tgcatcgcag
     2761 cttccaggca acccacagtt cgtgtcgaac agcgacgctc ataacctcga ctccataagt
     2821 ccacccagat gcgtgttcga gatcgaggag ctcagcgtcc atgaagtgct catggcgctc
     2881 cggcacgaga gtgggagagg cgtgagactc ctatgaggac aatcgcggac catgtcctgg
     2941 acatcgcctc gaacagcgtg acggctggcg ccggtgacat atggctggat atcgacgagg
     3001 atccggcgaa gcacctgctc actttcacgg tcaaggacaa cggacggggg atggatgccg
     3061 atatccgttc acgtgtgttc gacccgttct tcacgacacg gtcgcacgcc atcaggaggt
     3121 tcggccttgg cttgccgttc ctcaaggaga acacggagtt gacgggtggc ggggtcgatc
     3181 tcgagagtat cccggacgag ggaacaacac tcagggcaac ctttcacacc gaccacatcg
     3241 attgccttcc ttttggagac ttggcatcga gcatcttcgc cctgctcctc tccagtgata
     3301 gggtgagatg gcacatccat cgcgcgcggg gtgatgcaga ctacgccatc ggcaccgagg
     3361 aactggaaca ggtgttttcg ccggaagaac tgtctgatcc tcaatctcag tcgcttcttc
     3421 tcgagtttct gcgaggacag gaactcagtg tggccgagag tgatggtcgc gaattcctcg
     3481 agggaggggt ctagctgtgt cgacaacgac gaaatacgga ttcgaggaca tatcgaaggc
     3541 aaccatctct cgcatggcca cctacctccg ctgtctcacc tatcttgagc agcaaggcac
     3601 ggcgacagtg tcgtcccgcg aactggcttc gctgactggg gtgagccctg aacaggtaag
     3661 acaggacctg tcggtgtttg gccggttcgg caagcggggc accgggtatg acatcgtgcg
     3721 gctgaagacg gaggttgagg tcatcttcgg gcgtggcgtc gaacagtgga agtgctgcat
     3781 cgtgggtgtc gggtcgctcg gatcgtcgct tgtcgccagt cacatggttc agaagtgggg
     3841 attcaggtat gttgcggcgt tcgaccgtga tccgaacaag gtcggaacgc gtgtggagcg
     3901 catccccgtt tatcccgttg atgacctgaa ggcggtcgtc cgccgcgaac acatcgccat
     3961 tggtgttatt gccgttcctg cgtccagtgc ccgagcggtt gccaggaccc tctgcgacgc
     4021 aggcatccgg gggatcctca acttcgctcc ccagagtatc aaggtgcccg agaagatccc
     4081 ggtggtttca gtggacatct cacaggagtt cctcaagctg tcgttctata tccagagtgc
     4141 agagcagcga gcgcgcgagg aacagtcgca gatcgaggcg gcgtcgcata gcgagtagac
     4201 ggcttggcta ggacaccact cgctcagggt gcgagtagac gttcatgcgg ccgcctcgta
     4261 caaatgcgat aagcgtgctt ccgatcctct ccgcgagggc aactgcctga gcggtcgtag
     4321 cgctcctcga ggccaatacc ggtattccgg cgcgggccgc ctgggctacc gcctgagtgg
     4381 ggatgcgccc cgttcccatc aggaccgcct ctcccagatt ccacgagtgc gccacggcgt
     4441 aaccgatgac gatgtcgagt gccgtgtgac gactgatgtc ctcacaacag aagacgttgt
     4501 ctccctgtgc aacgagggct gcgtgaacgc ctcccgtagt cctccattga acagcgctgc
     4561 gctgcatctc ctcggccagg tctcgaaggc ggcgcgccga cacggtgaac ccacgtgaga
     4621 cagcgatatc ttcagccaac gcgtctccag aaatgcagtc gatgtcggtc gacatcggtg
     4681 agtccgggcc cggcgcgtca cgggagagag tgagaaacgc cgagcgtcct tccacggtga
     4741 ccacaaggat ctgagctggt cgtgcccagc cctcggaaac ggcgtggccg acggctgcgg
     4801 cttccatcag gtcgcccgag ctcgccaggc ggaccgccag cgtgccgttg acgtagatag
     4861 tcagcgggcg ttcttccgcg acctcatcct ggcattggac gacgttgccg ctcgggtcat
     4921 atctccagat acttgtcaac atggctggcc acctcccggg attgcatcca gtataggtcg
     4981 gttgcccccg gcagccagag gctgcgtgcc ccgtcagcct gcttgaatgt tgcattttcg
     5041 gtactgcaaa ggtatgataa acatgactat tagcgtcgtt tgcaaggagg ccccatgggc
     5101 agaatagctg agtactatcg tgcagcatcg ttggaggatg cgtttgccaa ggcgaagcag
     5161 tctggctcag ctgccgcgta tgtcggcggc ggcgtggagc tcgttcttcg ccggaacccg
     5221 gaggtaacga ccctcattga cctgtcacgt tgcggtctcg actacatcat agcagctggt
     5281 gagggcatcg agatcgggtc ccagaccacc ctcacaacga tcagtcgcag cgcggacatc
     5341 agacactacg ccggtggctc gcttgcgtgg tgggccggcc acatcgctca caataacctg
     5401 cgggacgtga tcacggtcgg cggagcgatc gggcgcaacc agccgtggaa cgatgtcgtg
     5461 ccccacctca ttgccctggg cgcacaggtc cggctgtttg atggcgtcga acaactcgtt
     5521 cctcttgccg gatacattga agccagacag ccgggtgtga tcatcaaggg cgt
//
LOCUS       DATMZD010000275         2302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_28149,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000275 DATMZD010000000
VERSION     DATMZD010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28149"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="VN478_05710"
     CDS             complement(<1..795)
                     /locus_tag="VN478_05710"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HWQ22203.1"
                     /translation="MEDVDILSVRDLLAILRRRWSIVVVTVLICVGAAGMLSYVVLSP
                     VYRSETSLVVTRRQPTPTGDSASYDQLQAYHALALTYAEIITSRAVLQDTIDTLRLPE
                     TVEQLAKMTAAEVAGDTGVILVSVRDTDSTRAALIANTVASSFVDQLPKLLSRNDSVS
                     VIDRAVPMTDQVTPRPLLNIAVALAGSLMLGIVLAFLVDSSDDTIRTTGDVQRLFGLR
                     VLAVVPDVRANGGHGNWGEGSLAFESFSALRTSLQFSMPDHEPQVIL"
     gene            1617..>2302
                     /locus_tag="VN478_05715"
     CDS             1617..>2302
                     /locus_tag="VN478_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22204.1"
                     /translation="MNRILALILCLVLGLAGVAVPAASAASISASSRTTLTLSLQQEY
                     LMRDTYQNIAAKYPTLTAFAAVATSEIATIASLKKVFSKYRVSVPVDANASVARTLAD
                     TATSVSNADTVAINLERATAVLMERLAQTTDNRDVATAVAAINTTSRGSHTTAFVSEQ
                     SIVSAPAPAPAPAPAPAPAPVAPAQHIASFTTGDSTATFLTLLADETVDVIEMAAGTY
                     HLRYTVINVD"
BASE COUNT          499 a          708 c          631 g          464 t
ORIGIN      
        1 aaggatcacc tgtggctcgt gatcaggcat cgagaactgg aggctcgtcc gaagagcact
       61 gaatgactca aatgccaggc tcccttcgcc ccagttgcca tgaccgccat tcgcacggac
      121 atcagggacg actgcgagca cccgaaggcc gaacagcctc tggacgtcac cagtcgtccg
      181 tatcgtgtca tcggaggagt ccacaagaaa cgcaagaacg atacccagca tgagcgaccc
      241 ggccaacgcc acagctatgt tgagaagggg ccgcggggta acctggtcgg tcattggaac
      301 agcacggtcg atgacactga cactgtcgtt tctgctcagg agcttgggca gctggtcgac
      361 aaacgatgaa gccacggtgt tggcgatcag cgcagcgcgc gtggagtctg tgtccctgac
      421 gctgaccaga atgactccag tgtcgcccgc cacctctgcc gcagtcatct tcgcaagttg
      481 ctctacggtc tccggaagcc tcagggtgtc gatggtgtcc tgcaggacgg cacggctggt
      541 gatgatctct gcatacgtga gagccagagc atggtacgcc tgcaactggt catagctggc
      601 ggagtctccc gtcggggtcg gctggcgcct ggtcaccaca agtgacgtct cagaccggta
      661 gacaggtgaa agcaccacgt agctgagcat gcctgctgca cccacgcaga taagcaccgt
      721 cacaacgacg atagaccaac ggcgacggag gatggccaga aggtccctca cgctcagtat
      781 gtctacatct tccatgatgg gaaccctctc cacgcgtttc gatatcaggg gttctccgcg
      841 ccaatacgtc cagcgtacca cgcggccata cgttccaata ctgcctgcac tacacatgtg
      901 ccagaggatt ccatgttcat caggtactga ttactttcta ccgttttccc ctggatttgc
      961 aagaggcaga ctcggccaac catcgcacga tgtgagccat tgccgactcg caagccggag
     1021 ccaacatgac ctctgggcgt ggcgccgtcg atcttgaccg aagcacttcg acctgtccgc
     1081 gttggatgtc agttgtctgc gattcgacaa gcccgctccc acgtgcaaca gaacagccgg
     1141 agccaggcca cgcagcaacc cggcaaggcg acaacacacc ggggagcgct tcacgaccag
     1201 cacagcagac aagagaccgc acggggtcgc ccggcgtgtg aggtgagtgg cgtgtccgga
     1261 agtcgacctg tcggagatcg gccgtctgca ggcgcgtctg cagactgtga atgtcgcttc
     1321 accttctcaa aaacgggaag aaggcgagcc agcgggtcat ctggaggcga gcctggtagt
     1381 gagaggagct tcgggagagt ctttcaggca tcatcacata ttcttcatga tcatacccta
     1441 tcgctatctc attcctgtgt tattattcgc ctgtaagaag tataggatcg accgcttgac
     1501 atgacgtgta aactgctgga cgtgacctct aaacttgctg caaaccgctt gtaagttgct
     1561 ggattctacc tgtaagtcgc aggacatgac ctttaaactt gctccacagg agtcagatga
     1621 acagaatctt ggccctcatt ctctgcctgg tacttggtct cgccggggta gctgtccctg
     1681 cagcgtcggc tgccagcatc tccgcttcaa gcagaactac tctcaccctg tcccttcagc
     1741 aggaatatct catgcgtgat acatatcaga acatcgccgc caagtacccg acgctgaccg
     1801 cgtttgctgc ggtggcgaca agtgagatcg ccacaatcgc ctcgctgaag aaggtcttct
     1861 cgaagtacag agtctccgtg ccggtcgacg ccaatgcttc cgtcgcccgg acacttgcag
     1921 atacagcaac cagcgtctcg aatgctgaca ccgtcgccat caacctcgaa cgggcgaccg
     1981 ccgtgctgat ggagcggttg gctcagacga ctgacaaccg tgacgtggca actgctgttg
     2041 cggctatcaa taccacttca cgggggtccc atacgacagc gtttgtcagc gagcagtcaa
     2101 tcgtctccgc tcctgcacct gctcctgcac ctgctcctgc acctgctcct gcacccgttg
     2161 cgcccgcgca gcacatcgcc tctttcacga ccggcgactc gactgcgacg ttcctgactc
     2221 tcctggccga cgaaacggtc gatgtgatcg aaatggcggc agggacctat catctccgct
     2281 acaccgtcat caatgtcgac ag
//
LOCUS       DATMZD010000276         7648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_58564,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000276 DATMZD010000000
VERSION     DATMZD010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7648
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58564"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="VN478_05720"
     CDS             <1..380
                     /locus_tag="VN478_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018126752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWQ22205.1"
                     /translation="EFIRALPDGYDTIVGERGYRLSGGEKQRIALARVILKNPRILIL
                     DEATSSLDSESEALIQDALNYVMAQRTSIVIAHRLSTILAADLILVIDRGHIVESGNH
                     ADLLAQGGLYANLYQTQFRTVNE"
     gene            complement(402..1307)
                     /locus_tag="VN478_05725"
     CDS             complement(402..1307)
                     /locus_tag="VN478_05725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HWQ22206.1"
                     /translation="MRRKIRHIRIIINPAAGTGGSILPTVNRFMEEVGIKWEASITHE
                     AGDAVKFAKLAVKEDADVLDVYGGDGTLMEAISGLIGSSVPLAILPGGSANVMAEELG
                     IPRDLRAACALMTQSPLASKTIDVGHFGSRYFILGVALGLGAEIIKGADRETKNRIGV
                     FAYLLSAAAALKKTRKAVYHLNIDGQKHEVQGLTCIIANVGNLGFTNISYDKHIDVCD
                     GLLDVLVVRKANLGLLKHVAVMLLKRERPDNLELVEHWQGKDITVSSSPRQAIQCDGE
                     VLKDMPLHIKIVPAAIKVLVPRKEG"
     gene            1678..2331
                     /locus_tag="VN478_05730"
     CDS             1678..2331
                     /locus_tag="VN478_05730"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HWQ22207.1"
                     /translation="MMPIGDDNSARRTFPLVTYALIALNVLLFLVELSGGDAFIMKWA
                     FVPSRFLANPGADFLTLFTSMFMHAGWVHLGGNMLYLWIFGDNVEDRFGHVKFVIFYL
                     LCGLAATFAQLAFSVGSKVPNLGASGAIAGVLGAYILLFPQGKVRVLQGQQVIQVPAL
                     IVIGLWIVLQFFSGVGSIAQSTDTGGVAYMAHIGGFVAGFVLTFLLRGSAAARPTSP"
     gene            complement(2568..3674)
                     /locus_tag="VN478_05735"
     CDS             complement(2568..3674)
                     /locus_tag="VN478_05735"
                     /inference="COORDINATES: protein motif:HMM:NF026253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HWQ22208.1"
                     /translation="MLCPNCGKIIPEGSTLCPGCGTGFSKEEAKATVLVPCRKCQKWG
                     FCDRDGKMVVEAIYDGARPFAEGLARVDLLTGSADRKVGFIDAAGNMAIPAIYDNTDD
                     FCGGLARVKLDGRYGFIDAAGNMAIPAIYDGADAFVEGFARVIGLNDQWGFVDVTGNM
                     AIPALYDAALPFKNGLARVNLKGKWGFVDATGKMAIPTIYDGAGDFSESLAHVNVWGK
                     WGFVDAGGNMTIAAVYDYADDFSESLARVELNDKRGFIDARGNVVIAVVYDDATSFCE
                     GLATVELDGKWGFIDAKGNMAIAAAYDHAVSFCEGLANVDVNGKWGYVDTLGNMVIPA
                     VYDRAATFCDGLALVEVDGQSGYIDTEGKQYWGS"
     gene            3946..5433
                     /locus_tag="VN478_05740"
     CDS             3946..5433
                     /locus_tag="VN478_05740"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWQ22209.1"
                     /translation="MEKETNGRPRTEDMSAQVAREVPEPTSGEELHVESVPLHSRIWV
                     SAADGATAILCSIAEGAALTYYFTRVMGLAPRLASIVWLLFGIWNAFNDPLFGYISDR
                     TRSGLGRRIPYIRYGAPLYAVAFAIFWIVLPGFKGNQTALFIQMLVGMFLFDTLYTAI
                     ATAVYIMPYEMAVSNKARSSIFIWKIGFSLISTAFPIAIGLIQPGPGQDATPYRVIMI
                     GLGVLMAGIIYASTFFYTENHFQQQEEQFNFWKSLGACFRNRSFVVFESVSFTVIYVQ
                     NQLMQGIYYYYDEVSMGVAKGTGTAFTLGSLFVGAVLGLLLFLNRRDVWGVKRCVRVF
                     ASMFGVGCIVGLLFAHQLIPMMVAFLLIGVGFAGGMYLIPLMNGDVIDADEHTTGLRR
                     EGMYAGVNSFVTKPAMSLAQAVFLAFLSHAGYDQSLAKGMQSASAEAGIVRAWFLVPA
                     ILLLACAVILKWYPLDGLSWEQIKTHLAVVHREKETKYLSEHGLK"
     gene            5455..6396
                     /locus_tag="VN478_05745"
     CDS             5455..6396
                     /locus_tag="VN478_05745"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWQ22210.1"
                     /translation="MPFLIVAAITTLLLIGAGWYFSGLVLRPRLFGYDETWDIEVDKG
                     RIDPAWWKSLPAEGVWIDSPFGYKIHAVWVQSSASKRTVILVHGITYTLWGSVKYMPL
                     FWNRAFNVLLIDNRHHGKSGGGDCTFGFRERWDVKACIDWVYGQTGQDSIVGLHGESM
                     GAAIVLQTAAVDPRIQFVVSDCAPADLSEELETRLSHDYRLPARLFVPIASLVTRLRA
                     GFWFSQVSPIHDVHSVEVPILFVHGQDDDYVPPQMAQRLYDAKANGLRRLYLAPHATH
                     AQSFWENKVDYDQIVGTFLEDAEIIEKAGPTQPEQAQ"
     gene            6500..7078
                     /locus_tag="VN478_05750"
     CDS             6500..7078
                     /locus_tag="VN478_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22211.1"
                     /translation="MLSREKRMRLWTYLLDPPVLAMLTFIFFYLMKPAERNIGACLTG
                     VVCLGLVPLSAWFHMVRHPADFAGERKVGFVLSIAGYVVGTLVMLIFFRSSRLNMAFM
                     LSYLFTVVGLTTVNLMHYKASGHASGAAGPATALTIVYGWPGAISFLLLVAVAVAKIN
                     IKEHTLGQLCTGASLSVASTLAGFAIMGLLHF"
     gene            7281..>7648
                     /locus_tag="VN478_05755"
     CDS             7281..>7648
                     /locus_tag="VN478_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWQ22212.1"
                     /translation="MNYDFEHGLERHNTGCLKWDQSCTVFGSEDVLPMWVADMDFPIP
                     KPVTEALVQRAQHECYGYSCASPSVLEAVTQRLWRNYQWKVDPEWLLFTPGVVPALHA
                     AVRAFTSPGDEVVLQSPVYYP"
BASE COUNT         1545 a         2285 c         2134 g         1684 t
ORIGIN      
        1 acgagttcat ccgtgcactc cccgatgggt acgacacgat cgtcggcgaa cgtggatacc
       61 ggctgagcgg aggcgagaag cagcgcatcg cactcgcccg ggtgatcctc aagaacccac
      121 gcatcctgat actggacgaa gccaccagta gcctggacag cgaatctgag gctctgatcc
      181 aggatgcgct gaactatgtg atggcacagc gtaccagcat cgtcattgcc caccggctga
      241 gcactatcct ggccgccgac ctgatcctgg tcatcgatcg ggggcacatc gtagaaagcg
      301 gtaaccacgc cgatctgctg gctcagggcg ggctgtacgc gaatctctac cagacacaat
      361 tccgaacagt caacgagtag ggacgtgcgg gatcctcagt gctatccttc cttcctggga
      421 accaggacct tgatggctgc cgggacaatc ttgatatgaa gaggcatgtc tttcaatacc
      481 tcgccatcac attggattgc ctgcctggga cttgaagaaa cggtgatgtc cttgccttgc
      541 cagtgctcca ccagctccag gttgtccgga cgctcccgtt tgagcaacat cacggccacg
      601 tgtttcagca ggccaaggtt ggccttgcgc acgacaagaa catccaggag tccatcgcag
      661 acatctatgt gcttgtcgta ggatatgttg gtgaacccga ggtttcccac attcgcgatg
      721 atgcacgtca ggccctggac ttcgtgcttc tgcccatcga tgttcaggtg gtagacagcc
      781 ttccgtgtct tcttcagtgc agcagctgca gaaagcaggt aggcaaagac cccgatcctg
      841 ttcttggttt cgcgatcggc ccccttgatg atctctgccc caagaccaag ggcaactcca
      901 aggatgaagt accggctgcc aaagtgcccc acgtctatgg tcttcgatgc cagcggcgat
      961 tgagtcatga gagcacaagc tgccctgagg tctctgggga tgccgagttc ctctgccatg
     1021 acgttcgccg accctcccgg cagaatggcc agaggtacgc tcgatccgat gaggccgctg
     1081 atcgcctcca tgagcgtgcc atcgccacca tagacgtcaa ggacgtctgc gtcttctttg
     1141 acggcgagct tggcgaactt gactgcgtcg cccgcctcgt gcgtaatcga tgcctcccac
     1201 ttgatgccaa cttcttccat gaaccggttg acggtgggaa ggatcgaccc tcccgttcca
     1261 gcggcagggt tgatgatgat gcgaatgtgt cgaatcttcc gtctcatggt atctgccccg
     1321 tcatggacgg attcccgtcc acgtagcctg cggtcaggac gtcggcacgt ccctgccaac
     1381 tctggcgccc gcccacatga gctcgattgc gccggacacg ttcatcatga gtttattttg
     1441 acgccatcac catgccgtgt caatctgacc ctctggattg ccatggagag gacctccttc
     1501 gagtacgcgg tctggtgaca caacaagtgc gcgcccacac gcaggactct tccctgtctt
     1561 cgcttgtgtt ggtgacagaa gtcgaccaca acgagatcgc ttttggagaa ccgtgctaga
     1621 attctcctaa tgtagggtcg agaccaatcc tgcccgatca aaaaggagat gagtcccatg
     1681 atgccaatcg gtgatgacaa cagcgcgcga agaacgtttc cgctggtcac ctatgctctg
     1741 atcgctctga acgtcctgct cttccttgtg gaactgagtg gcggagatgc cttcatcatg
     1801 aaatgggcct tcgttcccag ccgtttcctc gccaatcccg gagctgactt tctcacgctc
     1861 ttcacttcca tgttcatgca tgccggatgg gtccacctcg gcggcaacat gctctacctg
     1921 tggatcttcg gtgacaatgt ggaagaccgt ttcgggcatg tgaagtttgt catcttctat
     1981 ctgctctgcg ggcttgcagc gacctttgca cagctggcgt tcagcgtcgg atcaaaggtg
     2041 ccgaatcttg gagcatcggg agcgattgcc ggcgtgcttg gcgcctacat attgctcttc
     2101 cctcagggaa aggtcagagt actgcagggc caacaggtga tccaggttcc tgcgctgatc
     2161 gtcatcggcc tctggatcgt tctgcagttc ttcagcggcg tcggttccat agcccagtcc
     2221 acagacacgg gtggcgtcgc ctatatggca catatcggcg gattcgtcgc agggttcgtg
     2281 ctgacgttcc tgttgagagg cagtgcagca gctcggccga cctcgccgta gatcggtgca
     2341 acgcgagaca tccgacggaa cgggctgcaa cccgacggca tcgtggatct tcgattccga
     2401 aaggatctga tgagtacaga acctgccagg attctcctga gtgtcagaag atcggtgtcg
     2461 gtggacacct ctatcaacaa cagattggcc gactgtggag gatttctggc tgattttcgt
     2521 cgaccctgtc gtctcgtgga actcctgtcg gccgattcta ccgattctca gcttccccag
     2581 tactgcttgc cttcagtgtc gatgtagccg ctttgaccat cgacttcaac cagcgcgagg
     2641 ccatcgcaga aagtagcggc acggtcatag acggcaggaa tcaccatgtt ccccagagtg
     2701 tcgacatatc cccatttgcc attgacgtca acattcgcga gaccctcgca gaaggagaca
     2761 gcgtggtcat aggcggccgc gatggccatg tttcctttcg catcgatgaa gccccacttg
     2821 ccgtcgagtt cgacggtcgc gagaccctcg cagaaggagg tagcatcgtc gtagacaacc
     2881 gctattacta cgttgcccct ggcgtcgatg aaccctcgct tgtcattgag ttcgacacgc
     2941 gcaagggact cagagaagtc atccgcatag tcgtagacgg ctgctatcgt catgttgcct
     3001 ccagcgtcga cgaaacccca cttgccccag acgttcacat gcgcgagaga ctccgagaag
     3061 tcgccggcac catcatagat ggtcgggata gccatcttcc ctgtggcatc gacgaaaccc
     3121 cacttgccct tgagattgac gcgcgcaaga ccgttcttga atggaagggc ggcatcatag
     3181 agggctggga tagccatgtt ccccgtaacg tctacgaaac cccattggtc gttgagtcca
     3241 atgacgcgtg caaagccctc gacaaatgcg tctgcaccat cgtagatggc ggggatagcc
     3301 atgttccccg cagcgtcgat gaagccatac ctgccgtcca gcttgacacg tgcgagacct
     3361 ccacagaagt cgtctgtgtt gtcgtagatg gccgggatag ccatgttccc cgcagcgtcg
     3421 atgaagccga ccttgcggtc ggcgctcccc gtgaggaggt cgacacgcgc aagaccctcc
     3481 gcaaaagggc gtgcaccgtc gtagatcgcc tccacaacca tcttcccgtc tcgatcgcag
     3541 aatccccact tctgacactt ccgacagggg acaagcaccg tcgccttcgc ctcttccttt
     3601 gagaaccctg ttccacatcc ggggcagagg gtggatccct cgggaatgat ctttccacag
     3661 tttgggcaca gcatggagcg cctccttgtc agattgcacg gctggtggct cccgcacgag
     3721 aacgccagtg acagtaagaa tactactata tagtagacca aacctgcttc cccgcaaacc
     3781 atcccgccaa gtgagcaaca gagggccgac aagacgagga aaaccgcctt cttccctgca
     3841 agtcggatcc acgatgaggc agctcccggc tatgcaagaa acctgttgcc agtatactgg
     3901 ccatatcctg caggtccagt ggagaccgag ggtggagggg cagatatgga gaaggaaacg
     3961 aatggccggc cgagaaccga agatatgagt gcacaagtcg cccgggaggt tcctgaaccg
     4021 acatcagggg aagaactcca cgtagaatca gtaccgctcc acagccgcat ctgggtgtcc
     4081 gcagccgatg gcgcgaccgc catcctgtgt tcgatcgcgg aaggcgcggc gctgacgtac
     4141 tacttcaccc gtgtcatggg gctggctccg cgcctcgctt cgatcgtgtg gctgctcttc
     4201 ggcatctgga acgccttcaa cgatccgctg ttcggataca tcagcgaccg gacaagaagc
     4261 ggactcggcc gccgtattcc gtatatccgg tatggagccc cactgtatgc ggtcgcgttt
     4321 gcgatcttct ggatcgtcct gcctggcttc aagggcaacc agacggcact cttcattcag
     4381 atgctcgttg gcatgttcct gttcgacacg ctctacacag ccattgcaac agccgtctac
     4441 atcatgccct atgaaatggc agtctccaac aaggcgcgaa gcagcatctt catctggaag
     4501 atcgggttca gcctcatttc cactgccttt cccatcgcca taggactcat ccagcccgga
     4561 cccggtcagg atgccacacc ctaccgagtc atcatgatcg gacttggcgt cctcatggcg
     4621 ggcatcatct acgccagtac attcttctac acggagaacc atttccagca gcaggaggaa
     4681 cagttcaact tctggaagtc tctgggcgcc tgcttccgca acaggtcttt cgtcgtgttc
     4741 gagtccgtca gcttcacggt catctatgtc cagaaccagc tcatgcaagg catctattac
     4801 tactatgatg aagtgagcat gggcgtcgca aagggcacgg ggaccgcgtt cacgcttggc
     4861 tcactcttcg tcggggcagt actgggcctt ctgctgttcc tcaaccgtcg tgacgtctgg
     4921 ggtgttaagc gctgcgtacg cgtgtttgcg tcgatgttcg gtgtcggctg catcgttggg
     4981 ctcctgttcg ctcaccagct tatcccgatg atggtcgcgt ttctgttgat tggcgtcggc
     5041 ttcgccggcg gcatgtatct cattccgctg atgaacggcg acgtcatcga tgcagatgaa
     5101 cacacgacag gactgcggcg tgaaggcatg tacgccggcg tcaacagctt cgtcaccaag
     5161 cccgccatga gcctggcaca agctgttttc ctcgcgttcc tgtcacacgc aggctacgat
     5221 cagtcattgg caaagggcat gcagagtgcc tcagctgaag ccggcatcgt acgggcatgg
     5281 ttcctcgttc ctgcgatcct gctgctcgca tgtgcagtca tcctcaaatg gtatccactg
     5341 gacggcctca gctgggaaca gatcaaaact catctcgccg tcgtgcaccg cgagaaggag
     5401 accaagtatc tctctgaaca cggtctgaag tagagaactg ggagtcctct cagcatgcct
     5461 ttcctgattg ttgccgcgat taccaccctg ctcctgatcg gagcggggtg gtatttttcg
     5521 ggactcgtct tgcgcccgcg actgtttggc tatgacgaga catgggacat cgaggtggac
     5581 aagggccgaa tcgaccctgc atggtggaag agccttcccg ccgagggagt ctggatcgac
     5641 tcgccgtttg gctacaagat ccatgccgtc tgggtgcaga gctccgcgtc gaaacggacc
     5701 gtcatcctgg tgcatggcat cacctacacg ctgtggggat cggtcaagta catgccgctg
     5761 ttctggaacc gcgccttcaa cgtcctcctc atcgataacc gacaccatgg caagagcggg
     5821 ggaggggatt gcacgttcgg gttccgcgag cgatgggacg tgaaggcatg catcgactgg
     5881 gtctatggac agactggtca ggactccatc gtcggtctgc atggtgaatc catgggggcc
     5941 gccatcgtgc tccagaccgc tgcggtcgat ccgcgcatcc aattcgtcgt ttcggactgc
     6001 gcgcccgccg acctgtccga ggagctggag acacgcttgt cacacgacta caggctgccc
     6061 gcccggctgt tcgttccgat tgccagcctt gtgaccagat tgcgagccgg cttctggttc
     6121 agccaggtgt ctcccatcca tgatgttcac agcgtcgaag ttcctatcct gttcgtgcac
     6181 ggtcaggacg acgactatgt ccctccccag atggctcagc gactctacga tgccaaggct
     6241 aatggtctcc gccgcctgta ccttgcccca catgcgacgc acgctcagtc attctgggag
     6301 aacaaggtcg attacgatca gatcgtcggc acattcctgg aagacgcaga gatcatcgag
     6361 aaggccggcc cgacacaacc ggagcaggcg caataggcgg gacgtctcca tgaggtgcgg
     6421 aggcgcgccg cccattctgc actatacttg tctggtgagt ccctgactcg caagaactga
     6481 gaatcgaagg agtatcatta tgctcagccg agagaaacga atgcgtttgt ggacgtatct
     6541 tcttgacccg ccagtgcttg ccatgctgac attcatcttc ttctatctca tgaagccggc
     6601 cgagagaaac atcggtgcat gcctgacagg agtcgtctgc cttggtctgg tgccactcag
     6661 cgcctggttt cacatggtgc gtcatccagc cgacttcgcc ggcgaacgca aggtcggctt
     6721 cgtgctcagc attgccggct acgttgtggg aacgctagtc atgctgatct tcttccgcag
     6781 ttcacgcctg aacatggcat ttatgctttc gtacctgttc acggtagtcg gactcaccac
     6841 ggtgaacctc atgcactaca aggcctcagg acatgcttct ggagctgctg gtccagcgac
     6901 agcgcttacc attgtgtacg gttggcccgg cgcgatctcc tttcttctgc tcgtcgccgt
     6961 cgccgtcgcc aagatcaaca tcaaggagca tactctgggt caactgtgca caggagcgag
     7021 cctctcagtg gcctctacac tggctggctt tgcgatcatg ggcctgctgc acttctagca
     7081 ttgcactcct gacgaacagt gcgcgctgtt ttgcgtcgcg aaacatgctg acgcagaccg
     7141 gtttcgtcgt gtccaagctg tcctgccggg acaccgggcc gggcaaatgc tggtatactt
     7201 gcatggattg cctgcaggca tggccgcccc tgtcagacat gtcgatgtga tggcgccaca
     7261 ccggacaaaa ggagagggga atgaactacg acttcgaaca cgggcttgag cggcacaaca
     7321 cgggttgcct caagtgggat cagagctgta cagtctttgg aagtgaggac gttctgccga
     7381 tgtgggtcgc agatatggac ttccccatac caaaaccagt cactgaagcc ctggtacaac
     7441 gcgcccagca tgagtgctac ggctattcat gcgccagtcc gtcggtcctg gaggctgtca
     7501 cgcaacgcct gtggcgcaac taccagtgga aggtggaccc tgaatggctg ctgttcacgc
     7561 ctggcgtcgt gcctgcgctg catgccgccg tacgcgcgtt cacgagcccc ggggacgaag
     7621 tcgtgcttca gagcccagtc tattaccc
//
LOCUS       DATMZD010000277         2376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_139302,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000277 DATMZD010000000
VERSION     DATMZD010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139302"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..672)
                     /locus_tag="VN478_05760"
     CDS             complement(82..672)
                     /locus_tag="VN478_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22213.1"
                     /translation="MIGCTSWLVPGTWLENARVAKGLVDYDELLVASWDAETRSTLER
                     EIPDLLTLDLKYTVHLPYPDASHAWEAYDFFESREFPVLNYVLHPMAHWRDYAWGDRV
                     ALENLKETLEPCERMVFDVGHHLLGKRVPDEWKSRVVEVHAMGVRDGRDHLALDSATA
                     AVVLPWVADSTLLTFEVFDLDALVTSLHTWEACRAG"
     gene            complement(669..1157)
                     /gene="cobU"
                     /locus_tag="VN478_05765"
     CDS             complement(669..1157)
                     /gene="cobU"
                     /locus_tag="VN478_05765"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HWQ22214.1"
                     /translation="MILVTGGEKSGKSTYAMRTALDLGARRAFVATAEPFDDEMRARI
                     KHHREERGSRFETIEEPVEVPTVLRRCASYDVVLVDCMTTWLGNLMHYERDLASMKDE
                     LLSSISGNEIFVTNEVGMGIIPMDASTRFYVEELGRLNAALAQRAERVVFMVAGLPLV
                     LK"
     gene            1559..1819
                     /locus_tag="VN478_05770"
     CDS             1559..1819
                     /locus_tag="VN478_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22215.1"
                     /translation="MEIPMDTPPWLPFLVPLVALLCAIVVLAAMIVFGVTTARVARDK
                     GRNPVGWFFIGFFLLLSGLALALVMVPAGHCQEHARGPQTPA"
     gene            1960..>2376
                     /locus_tag="VN478_05775"
     CDS             1960..>2376
                     /locus_tag="VN478_05775"
                     /inference="COORDINATES: protein motif:HMM:NF025001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HWQ22216.1"
                     /translation="MTNQKGGVGKTTLAFHLATLFAADGKSVLALDLDPQANLTSCFL
                     RSDLPAENNSALLFEGRDPRTLTVGPGLDLVGTDSSLSKYEDHGASDHLESIGSWLDA
                     GDTHDVVIMDTPPAMGLFTRGALLAGDFAIIPADVSW"
BASE COUNT          456 a          731 c          701 g          488 t
ORIGIN      
        1 gtgaagtagc gggcgacgtg cttgacgttt cccttgccgc cgaagggcag gcggctcagg
       61 aaggagaagg ccagacgcgg ctcatccagc acgacaggcc tcccacgtgt gcagactcgt
      121 cacgagggca tcgaggtcga acacctcgaa cgtcagcagg gtactgtcag cgacccaggg
      181 caggacgaca gcggctgtcg ccgagtcaag cgcaagatgg tctctgccgt cgcgcacgcc
      241 catcgcgtgg acctcgacga cacgcgactt ccactcatcc gggacccgct ttcccaggag
      301 gtggtgtcct acgtcgaaca ccatgcgttc gcagggctct agtgtctctt tgaggttctc
      361 cagcgcgacg cggtcgcccc aggcgtaatc gcgccagtga gccatcgggt gcagaacata
      421 gttcagaaca gggaactcac gagactcgaa gaagtcgtac gcctcccacg catgagaggc
      481 atcagggtaa gggaggtgta cggtgtactt caggtcaagc gtcagcaggt ccggtatctc
      541 gcgctcgagt gtcgacctcg tttctgcatc ccagctggcg acaaggagct cgtcatagtc
      601 gaccagaccc ttggcgacac gggcattttc cagccacgtg ccagggacga gccaggatgt
      661 gcacccaatc atttcaggac gagaggcaga ccggcgacca tgaagacgac cctctctgca
      721 cgctgcgcca gagcagcgtt gagccgtccc agctcctcca cgtagaatcg ggtcgatgcg
      781 tccatcggga tgatacccat gccgacttcg ttggtcacga agatctcgtt gccggatatt
      841 gagctcagga gctcatcctt catggacgcc aggtcacgct cgtagtgcat gaggtttccg
      901 agccatgtcg tcatgcagtc cacaaggacc acgtcatatg atgcgcagcg gcgaaggacg
      961 gtgggcacct cgacaggttc ctcgatggtc tcaaaccgtg acccgcgttc ttccctgtgg
     1021 tgcttgatcc gggctcgcat ctcgtcgtcg aacggttcgg cagttgcgac aaaggcgcgg
     1081 cgcgcgccaa gatcgagtgc cgtgcgcatc gcgtacgtac tcttgccgga cttctcgcct
     1141 ccagtgacaa ggatcatcgt tcagtccctg tcgtgatgcc cgcacgctcg aacgtggctg
     1201 tcttctctgg agtctcccca gggacccaaa ggctgtctgt cggtctggtg agtctgtcga
     1261 gcccgttctg taaaccttct ctgagcattg cacatcctcc ggaaacgtca gtttctggtc
     1321 cggccgcatg cgatgagaac acgatcccac gcagtggtca ccggttgcat ccgctcttga
     1381 gggcggaccg cacttcacat gcccaacagc aaggcctgga cacacaactg gtcatcacag
     1441 ggcgggcacg tttcagtata cggcttttcc gccaccgtca acgtggcaag ttgcaccagc
     1501 tccgccgagt cgctattcta tgtctagtgc ggcaccgacc gctcgtgagg aggcacgcat
     1561 ggagattcct atggacacgc cgccgtggct accgtttctc gttcccctcg tggcgttgct
     1621 gtgtgcgatc gtagtgcttg cggccatgat cgtatttggc gtcacgacgg ctcgggtcgc
     1681 tcgtgacaag ggccgcaatc ccgtgggatg gttcttcatc gggttcttcc tgcttctctc
     1741 tggtctcgct cttgcgctgg tcatggtacc tgccgggcac tgccaggaac acgctcgcgg
     1801 accacagacc cccgcataga agcctcaggt acggccttcc gccatggact ttgtacatgc
     1861 gccctgcaag gggcggcagc atatacggat ggcacgcacc tgtgatatac tcacaagcac
     1921 acaaaccacg cggtgtcagg gggatctatg agaatcgtca tgaccaatca gaagggtggt
     1981 gtcggcaaga ctaccctcgc attccatctt gccacgctgt ttgctgcgga cggcaagagc
     2041 gttctggcgc tggaccttga cccgcaggcc aacctgacga gctgttttct gcgatccgat
     2101 ttgccggcgg agaacaactc tgcactgctg tttgaggggc gggatcctcg tactctcaca
     2161 gtgggccccg gtctggatct ggtcggaacc gacagcagtc tcagcaagta tgaggaccac
     2221 ggcgcctctg atcaccttga gtcgatcggc tcatggcttg atgccggaga cacgcatgac
     2281 gtcgtcatca tggacacacc gcctgccatg ggactgttca ccaggggagc acttctggca
     2341 ggcgactttg ccatcatacc tgctgacgta tcatgg
//
LOCUS       DATMZD010000278         3938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_148222,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000278 DATMZD010000000
VERSION     DATMZD010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148222"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..931)
                     /gene="ligA"
                     /locus_tag="VN478_05780"
     CDS             complement(<1..931)
                     /gene="ligA"
                     /locus_tag="VN478_05780"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF013790.4"
                     /note="this protein catalyzes the formation of
                     phosphodiester linkages between 5'-phosphoryl and
                     3'-hydroxyl groups in double-stranded DNA using NAD as a
                     coenzyme and as the energy source for the reaction;
                     essential for DNA replication and repair of damaged DNA;
                     similar to ligase LigB; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HWQ22217.1"
                     /translation="MDRDTARERIRELKAQIELHNYEYYVLDSPHVTDSAFDVLMREL
                     RSLEDQHADLVTPDSPTQRVGGAPLEGFTTVRHGVPMLSLSNMLSEEDIALFDTRVRR
                     ALGREEPVEYLTEVKMDGLAVSLRYEKGLLVEGSTRGDGMTGEDVTANVRTVRSIPMN
                     LASVGTHVPELVEIRGEMVMFRRDFEALNAARAVSDDPLFANPRNAAAGTLRQLDPRI
                     TASRPLHMVAYALGETSEDLVLPTQKSLLDWIASVGFRVSPFVRLVRTPAEMWSATRE
                     ILAARDSLPFAADGIVFKVNEIRLQQALGQTAKE"
     gene            complement(944..2095)
                     /locus_tag="VN478_05785"
     CDS             complement(944..2095)
                     /locus_tag="VN478_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22218.1"
                     /translation="MKKTVQPQGLRQLLLRNRRRLTVLAVVAAIAGAVTIWTHRGTRT
                     TLHVAAQFKTADYPVGISGTQDQGFVLTAAGQVFSLRGWTSQSEGVDVGDSPTSLTLS
                     TSGKYLLIAGQRLTLLDDALQQKWSRKTTDPSVVERTVFTLTGKIAVVYSFLANQSRQ
                     FVIYDLAGKYLNGYSVPDFGRGSQVSLARTGVLVVTLEGGTIYTVSPEGKVIAKFTVP
                     NPDQRLAGLVTAVNETGTRILAGYSRSISGIAEPLPRYLFDQTGKKIAETATTSENPG
                     LRAVGGAFLLFGRSAELLDTDCRQMVSASKLNFSVVDASTSDGECALLFQEQTTSQTA
                     VYFLGIYDIKTGALVRQWSTAESEVPRVFQLPGKRSVVALGSGISILIP"
     gene            2232..3677
                     /locus_tag="VN478_05790"
     CDS             2232..3677
                     /locus_tag="VN478_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HWQ22219.1"
                     /translation="MDKFSERAIAGAKRLGASYCDCRLIDMEREDVSVVGGAPRAVVH
                     AHQRGFGVRVIANGAWGFSSSSVLDEAEADRVVEEAVDIAHASALAKKTDVVLAPISP
                     GDDMVPNQADVDPFTISDSQKIGLLAEATRAMMLPGVVLARGQMQFVKERKIFASSEG
                     SYLVQDRIESGAGVSAMAASDKGDVQIRSYPMSFSGDNAQSGWEFVEAMRLLEHAEPT
                     AREALALLSAKPCPSGVRDLVVGGQQLALQVHESCGHPSELDRVMGMEASFAGTSFLT
                     PDKKGTFRYGSDIVNLTADPTIPHALGGLAYDDEGVKGRKVYLVRHGIFEHYLNSRAS
                     APVVGEEPMGAMRADGFSRSPIVRMVSINLEPGDLTFDQLIGGVDDGLYVDGIRSWSI
                     DDRRLNFQFGSEIGWEIRGGKLQDMVKNPTYTGIAYEFWRSCDGIGAPELYHVWGVDN
                     CGKGEPMQVAHVSHGTSPARFRHVRVGVLHE"
     gene            3670..>3938
                     /locus_tag="VN478_05795"
     CDS             3670..>3938
                     /locus_tag="VN478_05795"
                     /inference="COORDINATES: protein motif:HMM:NF013673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HWQ22220.1"
                     /translation="MSKEQLHDLMRDALKASSADQTEIVTMTSNGALTWYAENIIHQN
                     VAEHTAQVQVRAVVGKKVGSASTTDCSADGLALVVRQASEAARLMT"
BASE COUNT          764 a         1123 c         1250 g          801 t
ORIGIN      
        1 gttccttggc cgtctggccc agcgcctgct gcagacgtat ctcgttcacc ttgaatacga
       61 ttccgtcggc ggcgaaaggg agcgaatcgc gagcggccag gatctcccgg gtggccgacc
      121 acatctcggc aggcgtccgc acgagacgaa caaatggcga cacgcggaag ccgacgctgg
      181 caatccagtc aagcaggctc ttctgtgttg gaagtaccag gtcctcagag gtctcaccca
      241 gtgcatatgc taccatgtgc agcggacgcg aggcagtgat gcgtggatcc agctggcgca
      301 gggtccctgc ggctgcattg cgcggattgg cgaacagggg atcgtctgac acggctcgtg
      361 cagcattgag cgcttcgaag tcacgtctga acatgaccat ttcgccgcgg atctcaacca
      421 attcaggcac atgagtccca acgctggcca ggttcatggg gatggagcgc accgtccgga
      481 cgttggcggt gacatcttcg ccggtcatgc cgtctccacg ggtggatccc tcgaccagca
      541 ggcctttctc gtagcggagc gagacggcaa gtccatccat cttgacctcg gtcagatact
      601 caacgggttc ttcccgtccg agtgcccgcc gcacgcgagt gtcgaagaga gctatgtctt
      661 cctcggataa catgttgctg aggctgagca tcggaacgcc gtgccggaca gtcgtgaatc
      721 cttccagagg ggctccgccg actcgttggg tcggtgagtc gggagtaacc agatcggcat
      781 gctggtcctc cagactcctg agttcacgca tcagaacatc gaaggccgag tccgtgacat
      841 gcgggctgtc cagcacatag tactcgtagt tgtggagttc aatctgtgcc ttgagctccc
      901 gtatgcgttc acgcgccgtg tcgcggtcca cggatccctc ccgtcagggt atcagaattg
      961 agatgccgct gccgagggcg acgaccgaac gctttcccgg caactggaag acgcgaggga
     1021 cctcactctc agcagtactc cactggcgca ccaaagcacc agtctttatg tcgtagatac
     1081 cgaggaagta gaccgcggtc tgggaggtcg tctgttcctg gaacaggagt gcgcactctc
     1141 catcggaagt ggaggcgtcg accacactga agttgagttt ggacgctgac accatttgcc
     1201 tgcagtctgt gtcgaggagt tctgcgctgc gtccgaagag cagaaacgct ccgccaactg
     1261 cgcgaagacc agggttctcc gacgtggtgg ccgtctcggc tatcttcttc ccagtctggt
     1321 cgaacaaata gcgaggcagc ggctcggcga tgccggaaat gctgcggctg tagccagcca
     1381 ggatgcgcgt tccggtttcg ttgactgccg tgacgagtcc tgctagtctc tggtccgggt
     1441 tggggaccgt gaactttgcg atcacctttc cctctggcga gacggtgtag atggtgccac
     1501 cttccaacgt cacgacaagc acgccggtac gggccaggct gacctgcgac ccgcgtccga
     1561 aatcgggcac cgagtagccg ttcaggtact ttcccgcgag gtcgtagatg acaaactgcc
     1621 tggactggtt tgccaggaac gagtagacga cagcgatctt ccccgtgagg gtaaacactg
     1681 tccgctcgac gacgctggga tcagtcgtct tgcgtgacca cttttgctgc agggcatcgt
     1741 ccagcaatgt cagccgttgt cccgctatga gaaggtactt tcccgacgtg ctcagcgtca
     1801 gggatgtggg gctgtctcct acgtccacac cctcgctctg gcttgtccac ccacgcagcg
     1861 agaatacctg accggccgcg gtcaggacaa atccctggtc ctgcgtgccc gagatgccga
     1921 ccgggtagtc ggcggtcttg aattgagcgg ctacgtgcag tgtcgtccga gttccccggt
     1981 gcgtccatat ggtcactgca ccggcgatcg cagccacaac ggccaggact gtcagccggc
     2041 gccgattccg gaggagtagt tgccgcagac cctgcggctg aacggtcttc ttcatctcat
     2101 caccgtgaca attctagtct gaacgtcctg tttggcaaag aagacgcttg cgagagcaac
     2161 gccggccggt cctggcgttg cttttgacag atgctcccat atactgaccg tatcaaccag
     2221 ggaggtggcg ggtggacaag ttctcggaac gcgccattgc gggcgcgaag cgccttggtg
     2281 caagctactg tgattgcaga ctcatcgata tggaacgcga agacgtgtca gtcgtcggag
     2341 gagcgcctcg cgccgtcgta cacgctcacc agcgtggatt cggggttcgc gtcatcgcga
     2401 acggggcatg gggattctcc tcttcgtcgg tcttggacga agccgaagct gaccgtgtcg
     2461 tcgaagaggc agtcgacata gcacacgcga gtgcgctggc aaagaagacg gatgtcgtcc
     2521 tggcgccgat cagccccggc gacgacatgg tcccgaacca ggcggacgtg gaccccttca
     2581 cgatctcaga ctcgcagaag atcgggctgc tcgccgaagc caccagggcc atgatgctgc
     2641 ctggtgtcgt tctggctcga gggcagatgc aattcgtgaa ggagcgcaag atttttgcgt
     2701 cgtccgaggg ttcgtacctt gtccaggatc gaatcgagag tggtgcaggc gtgagcgcca
     2761 tggcggcaag tgacaagggt gatgtccaga ttcgcagcta cccaatgtcc ttttccggtg
     2821 acaacgccca gtcgggctgg gagtttgtag aagccatgag actgctggaa cacgctgagc
     2881 cgacagcgag ggaagcactg gcgctgctgt ccgccaagcc gtgcccgtcg ggcgtgaggg
     2941 atctggtcgt tggcggccag cagcttgccc tgcaggttca tgagtcttgc gggcacccgt
     3001 cggagctgga tcgggtcatg ggaatggagg cttcgttcgc ggggacatcg ttcctgacgc
     3061 ccgacaagaa ggggaccttc cgctacgggt cggatatcgt caatctgact gctgatccca
     3121 caattccaca cgccttgggt gggcttgcat atgacgacga gggagtgaag ggccgcaagg
     3181 tataccttgt gcggcatggc atcttcgagc actatctcaa ctccagggca tcagccccgg
     3241 tcgtgggcga ggagccgatg ggcgccatgc gtgccgacgg gttctctcga tctccaattg
     3301 tacgtatggt gagcatcaac cttgagccgg gtgacttgac cttcgaccag ttgattggtg
     3361 gggtggatga cggcctctac gtcgatggca tcaggagctg gtccattgac gatcgtcgcc
     3421 tgaactttca gttcggcagc gagattggct gggagatcag gggagggaag ctgcaggaca
     3481 tggtcaagaa cccgacctat acaggtattg cctatgagtt ctggaggtcg tgtgacggca
     3541 taggagctcc agaactgtat cacgtttggg gagtggacaa ctgcggcaag ggggagccga
     3601 tgcaggtggc gcacgtgtca cacggcacgt ctcctgcccg cttccgtcat gtccgggtgg
     3661 gggtgcttca tgagtaagga acagcttcat gacctcatgc gtgatgctct gaaagcgtcg
     3721 agtgcagatc agaccgagat cgtgacgatg acctcgaacg gcgcactcac gtggtatgca
     3781 gagaacatca tccaccagaa tgttgctgaa cacacggcac aggttcaggt tcgggctgtc
     3841 gtcgggaaga aggtcggaag tgcctctacg accgactgct ctgccgatgg actggcgctc
     3901 gtcgtgcggc aggcatccga ggctgcacgc cttatgac
//
LOCUS       DATMZD010000279         2118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_77625,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000279 DATMZD010000000
VERSION     DATMZD010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77625"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2118)
                     /gene="ileS"
                     /locus_tag="VN478_05800"
     CDS             complement(<1..>2118)
                     /gene="ileS"
                     /locus_tag="VN478_05800"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014452912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HWQ22221.1"
                     /translation="GYYVPRKGGWDCQGLPVEIEVEKRLHISGKQQIEEYGVEAFVQE
                     CRSSVFTYKAEWEKMTSRIGFWLDTEHAYATYTNDYIESVWHILKTFYDKGLLYQGHK
                     VVPYCPRCGTALSMHEVAQGYKSVTEETVFVKFSLTSDGLEGVKALVWTTTPWTLPSN
                     VALAVNPNVQYSVVELAGERYLVASARLAAVFGHDAEHVTRVRTYLGSELTGVRYERL
                     YDYARVEPWEEVRGWRIVTADYVTTTDGTGIVHLAPAFGEDDLSVGLKEGLPFVQLVD
                     PAGRFTSEVIPWAGKDAKGSDPEIRRQLKADGKLFKTERIEHDYPFCWRCETPLLYYA
                     KDSWFVRTTEFKDQMVANNQEITWYPEHIKNGRFGNWLENIKDWALSRERYWGTPLPI
                     WICDSCGHQHAVGSVAELTAMAVNPDPEVELHRPYVDKIELRCPVCGGTMHRVPEVID
                     VWFDSGSMPYAQWHYPFENKDEFAKMYPADFISEGIDQTRGWFYTLHAISTALNDRPA
                     FKRCMVLELILDEKGHKMSKHLGNVIDPWTILDKQGADAFRWSLYTSTPPWYPRRFGP
                     SVVADALKNFIIPLRNVYSFFVLYANIDHFDPNVAQLPCDKRPELDRWLLSRFQELVR
                     DVRSDMEAYDINPATKRIQAFVEELSNWYVRLSRRRFWRSENDTDKLSAYQTLYTVLV
                     DLARLLTPFTPFMSEEIYQNLVRS"
BASE COUNT          437 a          639 c          581 g          461 t
ORIGIN      
        1 cgaacgaacc aggttctggt agatctcctc tgacatgaac ggcgtaaacg gcgtcagcaa
       61 cctggcaagg tctaccagga ctgtgtagag cgtctgatag gctgatagct tgtcagtgtc
      121 attctcgctt cgccagaaac gtcgtctgct caggcgcacg taccagttgc tcaattcctc
      181 gacaaaagcc tggatgcgct ttgtcgccgg gttgatatca tacgcctcca tgtctgagcg
      241 cacgtcccgc accagctcct ggaaccggct cagaagccac cgatcgagct ccggtcgttt
      301 gtcacatggc agctgcgcca cgttcggatc gaagtgatcg atattggcgt acagcacgaa
      361 gaagctgtag acgttgcgaa gcggaatgat gaagttcttg agcgcgtcgg caaccacgct
      421 cgggccaaat cgcctcggat accagggcgg agtgctcgtg tagagagacc atcggaatgc
      481 gtctgccccc tgcttgtcga gaatagtcca cgggtcgatg acattgccga gatgcttgct
      541 catcttgtga cccttctcgt ccagaatcag ttcgagcacc atgcatcgct tgaacgccgg
      601 ccggtcgttg agggccgtgg aaatggcatg aagcgtgtag aaccacccac gtgtctggtc
      661 gattccttcg gagatgaagt cagcggggta catcttggcg aactcgtcct tgttctcgaa
      721 cggatagtgc cactgggcgt atggcatgga accagagtcg aaccagacgt caatgacctc
      781 cggaacacgg tgcatggtgc cgccgcacac tgggcacctg agttcgatct tgtcgacata
      841 cggtcgatgc agttcgacct ctggatccgg gttgacggcc atcgccgtca gttccgccac
      901 agaccccaca gcgtgctggt gcccgcagga atcgcagatc cagataggca atggagtccc
      961 ccagtagcgt tcgcgggaga gagcccagtc cttgatgttc tccagccaat tgccgaatcg
     1021 tccattcttg atgtgctccg ggtaccaggt gatctcctgg ttgttcgcga ccatctggtc
     1081 cttgaactcc gtcgtacgga cgaaccacga gtccttggca tagtacagca atggtgtctc
     1141 gcatcgccag cagaacggat aatcgtgctc gatcctctcc gtcttgaaca gctttccgtc
     1201 ggccttgagc tgacgtcgga tttcgggatc gcttcccttt gcgtccttgc cggcccacgg
     1261 gatcacttca gaggtgaacc tcccggcagg atccacaagc tgaacaaatg gaagcccttc
     1321 cttcaatcca acactgaggt cgtcctcacc aaacgcagga gccaaatgca cgatgccggt
     1381 tccatccgtc gtcgtcacat agtctgcagt gacgatgcgc cacccgcgaa cctcttccca
     1441 aggctcgact ctggcatagt cgtagagacg ctcgtatcga accccggtga gttcgctccc
     1501 aagatacgtt cggacgcgtg tcacgtgttc agcatcatga ccgaacacgg cggccagtcg
     1561 ggcggaggcc accagatacc gttcacccgc cagctccacg actgagtact gcacgttcgg
     1621 gttgactgca agcgccacgt tgctgggcag agtccagggc gtcgtcgtcc agaccagcgc
     1681 cttgacaccc tccagtccat ctgatgtcag tgagaacttc acgaagacgg tctcttcagt
     1741 gacagacttg tagccttgcg cgacctcgtg catggagaga gcagtaccac agcgcggaca
     1801 gtacggaacg accttgtgac cctgatacag caaccccttg tcgtagaacg tcttgaggat
     1861 atgccagacc gattcgatgt agtcgttggt gtacgtcgcg tacgcgtgct ccgtgtcgag
     1921 ccagaaaccg atgcggctgg tcatcttctc ccactctgcc ttgtaggtga agacgctact
     1981 cctgcattcc tgaacgaacg cctcgacgcc gtactcctcg atctgttgct tgccactgat
     2041 gtgcagccgc ttttcgactt cgatttcaac cggcagaccc tgacagtccc agccaccttt
     2101 gcgcggaacg tagtagcc
//
LOCUS       DATMZD010000280         4485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_77664,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000280 DATMZD010000000
VERSION     DATMZD010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4485
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77664"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..452)
                     /gene="rplN"
                     /locus_tag="VN478_05805"
     CDS             complement(84..452)
                     /gene="rplN"
                     /locus_tag="VN478_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HWQ22222.1"
                     /translation="MLRPYSHMIVADNSGAKEVRIITVLTNNKHQVGHVGDKCVVTVK
                     KAAPNAAVIKGAVVKCVIVRTKREVRRPDGSLVRFDQNAAVLIDDDGNPKGTRIFGPV
                     CRELRDKGYLKIASLAPEVV"
     gene            complement(445..714)
                     /gene="rpsQ"
                     /locus_tag="VN478_05810"
     CDS             complement(445..714)
                     /gene="rpsQ"
                     /locus_tag="VN478_05810"
                     /inference="COORDINATES: protein motif:HMM:NF012586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HWQ22223.1"
                     /translation="MSGEMTEKGIVLKAQEGRVVVGVKRMVRGSFGRITVRTTKLYAD
                     DSKRISSVGDTVVVKFARPISSSKRWRLIEVVTKVGTTVEGAQNA"
     gene            complement(714..932)
                     /gene="rpmC"
                     /locus_tag="VN478_05815"
     CDS             complement(714..932)
                     /gene="rpmC"
                     /locus_tag="VN478_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HWQ22224.1"
                     /translation="MRINKVRDMSDAELKVNMENLRRELFNLRFQLATRQLKNTARVK
                     EVRHDIAKILTVEHERTMGLASKKQEVK"
     gene            complement(932..1342)
                     /gene="rplP"
                     /locus_tag="VN478_05820"
     CDS             complement(932..1342)
                     /gene="rplP"
                     /locus_tag="VN478_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015755967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HWQ22225.1"
                     /translation="MLLPVRTKFRKMHRGRMRGMATRGAELSFGEFGLQAMECSLVTD
                     RQIEAARLSLVSAVHKKGKMWIRIFPDKPITKKPAETRMGSGKGDVDHWAAVVKPGRI
                     LFEMSGISEEEARRVTRLASFKLPIRTRFLSRGS"
     gene            complement(1359..2024)
                     /gene="rpsC"
                     /locus_tag="VN478_05825"
     CDS             complement(1359..2024)
                     /gene="rpsC"
                     /locus_tag="VN478_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013412377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HWQ22226.1"
                     /translation="MGQKVHPYGFRLGITQDWESKWFASKKNYSELVLEDYHIRREIG
                     KIERDFAISEIEILRKGFNVTVRLHSARPGALIGRNKAELEKIEANLKKTLTNKQEVL
                     HVDVKEIKHPDLDAKILAQSIVADIEKRVSYKRAMKQVIKRAMRARAGGIKVQCSGRL
                     GGAEIARTEWYREGRVPLATLKADVAYSYEPALIKFGRIGVKVWLYKGDAPKRVFFSE
                     DVD"
     gene            complement(2025..2366)
                     /gene="rplV"
                     /locus_tag="VN478_05830"
     CDS             complement(2025..2366)
                     /gene="rplV"
                     /locus_tag="VN478_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HWQ22227.1"
                     /translation="MEAFAKLEHVRLSPFKTRLVADLIRGKNVDQAVAILDSLPNRPA
                     VTIKKVMLSAAANAENNFKMNRDTLYVSKILIDGQGGVKRVSPRGMGRADIIRKPLSR
                     IYICVAEKEEA"
     gene            complement(2374..2658)
                     /gene="rpsS"
                     /locus_tag="VN478_05835"
     CDS             complement(2374..2658)
                     /gene="rpsS"
                     /locus_tag="VN478_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HWQ22228.1"
                     /translation="MSDTKTNKGFVEERLLGRILEMNRKGEKKLVKTWSRRSFVVDEM
                     VGHTIAIHNGKTHVPVYISKAMVGHRLGEFALTRNFHGHTVPTARTSALR"
     gene            complement(2669..3499)
                     /gene="rplB"
                     /locus_tag="VN478_05840"
     CDS             complement(2669..3499)
                     /gene="rplB"
                     /locus_tag="VN478_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HWQ22229.1"
                     /translation="MSIVRSYKPTSPGIRSRKTLIHTEVTRDTPEKSLTFGLRKQAGR
                     NNTGKIMVRHHGGGVRQLYRVVDFARDVDDVSGKVAEIEYDPNRSALIALVQYANGKK
                     TYILLPEGLRVGDTIMSGENVEIKPGNNTKLAKMPLGTTIHNIELFPGKSAHVARAAG
                     ASAQLLAKEAGWAQVRMPSGEIRKFSLDCRATIGQVGNLDHNKEVLGKAGSSRHRGIR
                     PSVRGVAMNPIDHPHGGGEGKSPVGHAGPLTPWGKPTLGYKTRKKKNQSDKYILKRIN
                     "
     gene            complement(3511..3792)
                     /gene="rplW"
                     /locus_tag="VN478_05845"
     CDS             complement(3511..3792)
                     /gene="rplW"
                     /locus_tag="VN478_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HWQ22230.1"
                     /translation="MSKEILLYPVVTEKSMGGTEEGRYEFIVVKNSTKMEIKDAVENM
                     FKVKVAWVNVLRTHGKKRMRKASIGMTPSKKKAVVSLMPGFKIDVITNA"
     gene            complement(3789..4412)
                     /gene="rplD"
                     /locus_tag="VN478_05850"
     CDS             complement(3789..4412)
                     /gene="rplD"
                     /locus_tag="VN478_05850"
                     /inference="COORDINATES: protein motif:HMM:NF012782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HWQ22231.1"
                     /translation="MSTVKMIDVTGADAGEVELAEGLFGCSISETAMYMGVKKFLAGL
                     RRGTANTKGRSEINKTKKKVWKQKGTGHARQGNKRAPQWKGGGVVFGPKARDFTLDLN
                     KSTKRSAILSSLTQRAGENAIVVLRGLKLEQPKTKALLTALKAAGIPENALLVFAEEN
                     GEVVLSGLNLPRLGLLHFSELNAYDVLLHSTVVFTEEALKGAEEVYA"
BASE COUNT          903 a         1344 c         1136 g         1102 t
ORIGIN      
        1 cccttgtcct tgccggagag gacgacgact gtgtcgcctt tcctgatatt catgaacttt
       61 atagtaccct tcatggtcaa ctcctacaca acttccggtg cgagcgaggc aatcttgaga
      121 tagcctttgt cacgcagctc ccggcagacc ggtccaaaaa tacgtgtccc cttcgggttg
      181 ccatcgtcgt cgatgagaac ggcagcattc tggtcgaaac gtaccagact gccatcgggt
      241 cgacgaactt ccctcttcgt cctgacgata acacacttga cgaccgcacc cttgatgact
      301 gctgcatttg gagcggcctt cttcacggtg accacgcact tgtcgccaac gtggccaacc
      361 tgatgcttgt tgttcgtgag aaccgtgatg atcctgactt ccttcgctcc ggaattgtca
      421 gcaacgatca tgtgactgta aggcctaagc attctgagcc ccctcgacag tcgtccccac
      481 cttcgtcacg acctctatca gtctccagcg cttggagctg gaaataggcc gggcgaactt
      541 gaccacgaca gtgtcaccaa cagaggaaat gcgcttgctg tcatctgcgt agagtttagt
      601 cgtacgcaca gtgatacggc caaaactgcc tctgaccatg cgcttgaccc caaccacgac
      661 acgcccttcc tgggccttca gcacgatccc cttctcagtc atctctccag acatcacttg
      721 acctcctgct tcttcgacgc gagacccata gtgcgctcgt gctcgaccgt caggatcttg
      781 gcaatgtcat gacggacttc cttcacgcgg gccgtgttct taagttggcg cgtcgcaagc
      841 tgaaatctca agttgaacag ctctcgacga aggttctcca tgttcacttt gagttcggca
      901 tctgacatgt ctctcacttt gttgatcctc attagctccc ccttgataag aagcgtgtcc
      961 tgatgggcaa cttgaaggaa gccagtctgg tgacccggcg agcctcttct tcgctgatac
     1021 cagacatttc gaagaggatt ctccccggtt tgacgacggc ggcccaatgg tcgacgtcac
     1081 ctttgcctga acccatacga gtctccgcag gtttcttcgt gataggcttg tcggggaaga
     1141 tgcgaatcca catctttccc ttcttgtgaa cggccgagac aagagacaga cgtgctgcct
     1201 cgatctgacg gtccgtcacc agggagcact ccatggcttg cagcccgaat tcgccgaacg
     1261 aaagttcggc tccacgagtg gccatgccgc gcatccgtcc acggtgcatc tttctgaact
     1321 tcgttctcac aggtaatagc atgatgacct ctccttgctc agtctacgtc ctcactgaag
     1381 aagaccctct tgggtgcatc tcccttgtag agccacacct tgactccaat acggccaaac
     1441 ttgatgaggg ccggctcata ggagtaggct acgtcagctt tcaaggtcgc aagcggaacg
     1501 cgtccttcac gataccattc cgtgcgggcg atctcggctc cgccaagacg gccgctgcac
     1561 tgaaccttga taccgcctgc gcgagctctc attgcacgct tgatgacctg cttcatggcg
     1621 cgcttgtagg agacgcgctt ctcgatatcg gcaactatgc tctgtgccag aatcttggcg
     1681 tcgagatcgg ggtgcttgat ctccttgaca tcgacgtgca ggacttcctg cttgttcgtc
     1741 agtgtcttct tgaggttcgc ctcgatcttc tcgagctctg ccttgttcct tccaataagc
     1801 gcgcctggac gcgcgctgtg caaacgcaca gtgacattga aacccttcct caggatctcg
     1861 atctctgaga tggcaaagtc gcgctcaatc ttgccaatct cccggcgaat gtgatagtcc
     1921 tccagaacga gttcgctata gttcttcttc gacgcaaacc acttggactc ccagtcttgg
     1981 gtaataccga gtctgaatcc gtacggatgt actttctggc ccacctaagc ctcctctttc
     2041 tcagctacgc agatatagat ccgggagagg ggcttgcgga tgatatccgc tcgacccatg
     2101 cctctcgggg agactctctt gactccaccc tgcccgtcga tgagaatctt ggacacatac
     2161 agcgtgtcac ggttcatctt gaagttgttc tcggcattgg cagcggcgga caacatgacc
     2221 ttcttgatcg tcacagcagg tcgattgggc agcgagtcga gaatagcgac tgcctggtca
     2281 acattcttcc ccctgataag gtcggccacc aggcgtgtct tgaaaggaga aagccgaacg
     2341 tgttcaagct tcgcaaatgc ttccatatgg cacctacctc aatgcgctag tgcgcgcagt
     2401 cggaacagtg tgaccgtgga agttgcgggt cagggcgaac tcaccgagcc tgtgtccaac
     2461 catggctttc gagatgtaga cagggacatg tgtcttgcca ttgtggatgg caatcgtgtg
     2521 tccaaccatc tcatcaacga cgaaagaacg acggctccat gtcttgacaa gcttcttctc
     2581 gccttttcgg ttcatttcca ggatcctgcc gagcagacgc tcctcgacaa agcccttgtt
     2641 ggtcttcgta tcgctcatcg gcgttctcct agtttatacg cttcaagata tacttgtcgg
     2701 actggttctt cttcttgcgg gttttgtagc caagagttgg cttgccccaa ggagtgagag
     2761 gtccagcgtg tccgacagga gacttgcctt caccaccacc gtgcgggtgg tcaatggggt
     2821 tcatggcaac accacgtacg ctcgggcgga tgccgcggtg acgcgaactg ccggccttgc
     2881 ccaagacctc cttgttgtga tcaagattgc ctacctgtcc gatggttgct ctgcagtcca
     2941 gcgagaactt gcggatctct ccggatggca tgcgaacctg cgcccatcca gcctcttttg
     3001 caagaagctg tgctgaagca ccagcggccc gtgccacgtg agccgacttg ccggggaata
     3061 gctcgatgtt gtgaatggtg gttcccaacg gcatcttggc aagcttggtg ttgttgcccg
     3121 gcttgatctc tacgttctcg ccagacatga ttgtgtcgcc gactctcagg ccctcgggca
     3181 gaagaatgta cgtcttttta ccgttggcgt actggaccag cgcgatcagt gcggaacggt
     3241 tcggatcgta ctcgatctca gctactttgc cagagacatc gtctacgtcg cgcgcaaagt
     3301 caacgacgcg atacagctgg cggacgccgc cgccatggtg acgaaccatg atcttgccgg
     3361 tattgttacg cccggcctgc ttgcgcaagc caaaggtcag ggacttctcg ggagtgtccc
     3421 gcgtgacttc ggtgtggatg agcgtcttcc tggaacggat tcccggagac gttggcttat
     3481 atgatcttac aatactcatg gggcaccctc ttaagcgtta gtgataacgt cgatcttgaa
     3541 cccaggcatc agagaaacga cagctttctt cttcgaaggc gtcatgccaa tcgaagcctt
     3601 ccgcattctc ttcttgccat gagtcctcaa aacgttaacc caggcgactt tcaccttgaa
     3661 catgttctct acggcatcct tgatctccat cttcgtggag ttcttgacga caatgaactc
     3721 gtatctgccc tcttcggttc cgcccatgct cttctcggtc acgacagggt acagcaggat
     3781 ctctttgctc atgcatacac ctcctcggcg cccttcaggg cctcttcggt gaaaacgacc
     3841 gtggaatgca ggagcacgtc gtatgcgttc agctccgaga aatgaagcag gccaagccga
     3901 ggcaggttga ggccagaaag aacgacttcg ccgttctcct cagcaaagac aagcaacgca
     3961 ttctcgggaa tacccgcggc cttcagcgcc gtcagaagcg ccttcgtctt gggctgctcg
     4021 agcttgagtc cacgcagaac tacaatcgcg ttctcgccgg cccgctgggt cagagagctg
     4081 aggattgcac tacgcttggt cgacttgttc aggtcaaggg tgaagtctct ggccttggga
     4141 ccgaagacga ctcctccacc cttccactgc ggcgcgcgct tgttcccctg acgcgcatgc
     4201 ccagtaccct tctgtttcca gaccttcttc ttcgtcttgt tgatctcact gcggcccttg
     4261 gtgttggcag ttccacgacg caatcccgcc agaaacttct tgacacccat gtacatggct
     4321 gtctcactta tgctgcagcc aaagagcccc tcggcgagtt cgacttcacc agcatcagcg
     4381 ccggtaacat caatcatttt gacagtactc atctctctac ctctcaagcc ctgatcgtca
     4441 ggagagcgtt gtctccgcca ggaatactgc ccttgactac gagca
//
LOCUS       DATMZD010000281         2017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_128636,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000281 DATMZD010000000
VERSION     DATMZD010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128636"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VN478_05855"
     CDS             <1..229
                     /locus_tag="VN478_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22232.1"
                     /translation="FENAAFLGAVSLRDYRSAGADASRSVASAMRDGEGVLLVVLRFG
                     PETTGLSRLFRQAVLTLRGPLVSGKPNPSDA"
     gene            complement(218..1537)
                     /locus_tag="VN478_05860"
     CDS             complement(218..1537)
                     /locus_tag="VN478_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWQ22233.1"
                     /translation="MRTGHGNRWFVFGTFFAFMLLHQADMLLIGPLTTPIMETFHIDE
                     AQMGAVVTGALIVGGILYPVWGYLYDRYSRPKLLALASFMWGSTTWLSALAPTYPLFV
                     ATRASTGIDDASYPGIYSLLADYFAPAVRGRIYGLLQITSPFGYIVGMVLATALKDIV
                     GWRGVFYVTGSLGIVVSVVILLGVRDTARGSSEPELQGVAMLQERRFDWHTAGRLLRK
                     KSLILLYLQGFFGVFPWNVITYWFFRYLEKGRGYSSGDVLSTMIVAILVLAAGYPLAG
                     ALGDRLFCKTIRGRLIVSIVGIVAGTVLFFITMNVPVTARTTFLVMLAASALFIPFAS
                     PNVVSTVCDVTLPEVRSTSVAIQNFVESAGAALSPLIAGLIAVKLDLRTAIVLICTST
                     WAVCALLFAGAIILVPRDIEAVRAELRLRAQAEQTLLQPPEKSGSSV"
BASE COUNT          437 a          655 c          608 g          317 t
ORIGIN      
        1 cttcgagaac gcagcattcc ttggtgccgt cagcctgaga gactatcgct ccgccggagc
       61 cgacgcctca cgctcggtcg catcggccat gcgtgacggc gagggggtcc tcctcgtcgt
      121 tctccgcttc ggcccggaaa cgacagggct gtccaggctg ttccggcagg cggtcctgac
      181 cctcagaggc ccactcgtgt cgggaaagcc gaacccgtca gacgcttgat cccgacttct
      241 ccggtggctg cagcagagtc tgttcggctt gcgcgcgcag tcgcagctcg gcgcgcaccg
      301 cctctatgtc gcgcggcacc aggatgatgg cgcctgcgaa cagcagggcg cagacggccc
      361 atgtcgacgt acagatgagg acaatcgccg tccgcaggtc cagcttcacc gcgatgaggc
      421 ctgcaatgag cggcgagaga gccgcccctg cactttccac gaagttctgg atagcgaccg
      481 acgtgctgcg cacctcgggc aatgtcacgt cgcacacggt cgagacgacg ttcggtgagg
      541 caaacgggat gaaaagagcg gatgcggcga gcatcaccag aaacgtggtc ctggctgtga
      601 cgggaacgtt catggtgatg aagaacagca cggtaccagc gactataccg acaatactga
      661 caatgagacg cccgcgtatg gtcttgcaga acaggcggtc tcccagcgcc ccggcgagag
      721 gataccctgc cgccagcacg aggatggcga caatcatggt cgaaaggaca tccccgctgc
      781 tgtagccgcg tcccttctcc agataccgga agaaccagta cgtgatgacg ttccagggaa
      841 ataccccaaa gaagccctgc aggtagagaa ggatcagcga cttcttccgc agcagccgac
      901 ccgcggtatg ccagtcgaag cggcgttcct gaagcatggc gacgccctga agctcaggct
      961 cagagctccc gcgggcagtg tcgcgcaccc cgagcaggat caccaccgag accacgatgc
     1021 ccagcgatcc agtcacatag aatactccac gccatccgac gatgtccttg agcgccgtgg
     1081 caagaaccat gcccacgatg tagccaaacg gcgacgtgat ctgcagcagc ccatagatgc
     1141 gaccacgtac cgcgggggcg aagtagtctg cgagcagcga gtagatgccg gggtagctgg
     1201 cgtcgtcgat gccggtcgac gcacgtgtgg caacgaacag aggataggta ggcgccaggg
     1261 ccgacagcca tgtggtcgag ccccacatga acgaggccaa cgccagcagc ttcggtctcg
     1321 agtaccggtc gtacaggtat ccccagacag gatagaggat gcctcctacg atgagtgctc
     1381 cagtgacgac ggcgcccatc tgggcctcgt cgatgtggaa cgtctccatg atcggcgtcg
     1441 tgagcggacc tatcaggagc atgtcggcct gatgaagaag catgaacgca aagaaggttc
     1501 caaacacgaa ccaccggttc ccgtggcctg tccgcatcac tccccccaaa tgcacgtcat
     1561 acgttcccag tgtatgaagg gaaaccgaca atacaaacgt ccagcgatga tctcctgaga
     1621 ctggaacaca tcaattccac agacgatcca tgggcggtgg acggtccagg gcagtctccc
     1681 cggccgtgac ccacgcatcg ctgccgaccg acatacttcg tcctcctggc atcacagcct
     1741 gcgcgtccag aagaacaaca acgcccccgg gggaggaggc cggctgtcgt cggcctaacc
     1801 gggggcatct gaagcccgag agggtggcgt gcctgctgca gcaacgccac ttgcagagga
     1861 ggctgccggg agtcttgtcg gctcaccgac acatccggtg accagtccgc tcccgcagtc
     1921 ctccgccttc acctacacaa acaccccaac gccctgtcca ctcctcacga cgcgctccct
     1981 ggtgggaggc agccgtcgac gaccgacgca acgggtc
//
LOCUS       DATMZD010000282         2197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_19015,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000282 DATMZD010000000
VERSION     DATMZD010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19015"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="VN478_05865"
     CDS             <1..380
                     /locus_tag="VN478_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22234.1"
                     /translation="LHIPVNYAVFAGLCSEGWFSLWNPSAPLAYFDGLRDGYLALMRV
                     SRLDSEIPEQLLEHGRSGANFIYYLDPPVTVSRLHPVVKPHLYEQYVADLRAYLSEHG
                     WLLGEEGPTASGEPETESLFDTL"
     gene            complement(523..900)
                     /locus_tag="VN478_05870"
     CDS             complement(523..900)
                     /locus_tag="VN478_05870"
                     /inference="COORDINATES: protein motif:HMM:NF020950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HWQ22235.1"
                     /translation="MNKTTAVKQVITLALIVLVLVVIIPLVKRSFKSVPGLEARVGET
                     FTVTLDSSADAAFRWDLLATDETKVQLVDHPDAKEAIASTEVWTFRALAPGKVTLYFG
                     YLRLDSPNSPAIRTHSVQLTVNP"
     gene            complement(947..>2197)
                     /locus_tag="VN478_05875"
     CDS             complement(947..>2197)
                     /locus_tag="VN478_05875"
                     /inference="COORDINATES: protein motif:HMM:NF001989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HWQ22236.1"
                     /translation="DGSCQVVLMGTVLGEGSTELYRERGTALWTAVTAALDRLRSAAD
                     LVIIEGAGSIAELNMTEHDMVNMSVARYAHAPVLLVGDIERGGVFAQLLGTLWLLPPE
                     DRACVKGLIVNKFHGDMSLFDDGVRILQERSGLPVLGVIPYVEDLALPEEDAACLDTP
                     VTERAAEGDIDIAVIRLPHIANFDDFDPLATEDGVHVRYVSSPRELGTPAAVILPGTR
                     STMGDLAWLHETGLADAILDLARGRTPVAGICGGYQMLGSRVRDPDHVESSLDEMPGL
                     GLLPHVTTFRGVKTTRQVQARVMSSVAWLSSLAGQTLHGYEIHMGTTQGADPWLSLTR
                     CGDKSAGITDGAITPNGRIWGCYVHGLFANESARRTWLTSLGWGGSTCGKRSEGHARS
                     LDRLADTVLCALDMQRLQQIIEDQ"
BASE COUNT          407 a          680 c          687 g          423 t
ORIGIN      
        1 atctgcacat tcccgtcaac tacgcggtgt tcgcgggatt gtgctctgaa ggctggtttt
       61 ccctgtggaa cccatcggct ccgttggcct actttgatgg tcttcgcgat ggctatcttg
      121 cgctgatgcg agtctcacgc ctcgactctg agatccccga gcaactgctc gagcacggca
      181 ggtccggcgc caacttcatc tactatctcg acccacctgt caccgtaagc aggcttcatc
      241 cagtcgtgaa acctcatctc tacgagcagt atgttgcgga cctgagagca tatctctcgg
      301 agcatggatg gctccttggc gaggagggtc cgacggcgtc aggagaaccg gagaccgaaa
      361 gcctgttcga tactctctag ggaaatgcac cgtcaacatg ctcgaattcg ccacctggtt
      421 gcgttcgcac tcatcgacga tgggcgtgac cctcatgtct ggcgccgatc agacctggca
      481 gctgcgcatg caaggaactg gaaccgcttg gtgaacgcct cttcagggat tgacggtcag
      541 ttggactgag tgcgtccgta tcgcaggcga attgggagaa tcgaggcgga gatagccgaa
      601 gtacagggtg accttgccgg gagcgagggc tcggaaggtc catacttcgg tactcgcgat
      661 tgcttccttc gcgtcgggat ggtcgacgag ctgcaccttt gtttcgtcgg tagccagcag
      721 gtcccaccgg aatgccgcat cagcactgct gtcaagcgtc accgtgaacg tctcgccgac
      781 gcgagcctca agcccgggga ccgacttgaa cgaccgcttg acgagcggga tgatgacgac
      841 aaggacgagg acgatcagtg ccagcgttat gacctgcttc accgcggttg tcttgttcat
      901 agcggacagt atatgccgat gtggtgcaca tgcacgcgaa gccgcgctac tggtcttcga
      961 tgatctgctg gagtcgctgc atgtccagcg cgcagaggac tgtgtcagcc aggcggtcca
     1021 gcgaccgggc atgcccttcg gaccgttttc cacatgtcga cccaccccag cccagggagg
     1081 tcaaccacgt ccggcgcgca ctctcgttgg cgaagaggcc atggacgtag cagccccaga
     1141 tgcgaccgtt cggagtgatt gccccgtccg tgattcctgc cgacttgtcc ccgcaccggg
     1201 tcaggctgag ccatgggtcg gctccttgcg tcgtacccat gtggatctca tatccatgga
     1261 gggtctgacc ggcgagagat gacagccagg cgacggacga catgactctg gcctgtacct
     1321 ggcgggtcgt cttgacgccc ctgaacgtcg tcacgtgagg caggaggccc agaccgggca
     1381 tctcgtccag cgaggactcg acatggtccg gatcacggac acgagagccg agcatctggt
     1441 agcccccgca gatacccgcg accggagttc ggccccgtgc caagtcgaga atggcatcgg
     1501 ccagacctgt ctcatgcagc caggcgaggt ctcccatcgt gctccgtgtc ccgggcagga
     1561 tgacggcggc gggcgttccg agttcacggg gcgaggacac gtatcgcacg tgaacgccgt
     1621 cttccgtcgc caacgggtcg aagtcgtcga agttggcgat gtggggcaga cggatgacag
     1681 caatgtcgat gtctccctcc gctgcacgct cggtgaccgg agtgtccagg caagctgcat
     1741 cctcctcggg cagtgcgagg tcttcgacat aggggatgac gccaaggacc gggaggccgg
     1801 agcgttcctg caggatcctg acgccgtcgt cgaacagcga catgtcgccg tgaaacttgt
     1861 tgacgatgag gcccttcacg cacgcgcggt cctcgggcgg cagaagccac aacgtgccga
     1921 gcagctgggc gaagacgccg cctcgctcga tgtcgccgac cagcagtacc ggcgcgtgag
     1981 catacctcgc cacgctcatg ttgaccatgt cgtgctcagt catgttgagt tctgcgatac
     2041 tgcctgcgcc ctctatgatg accaggtcgg cggctgaccg caggcggtcc agggccgcgg
     2101 tcacagccgt ccagagcgca gtgccccgtt cgcggtagag ctcggtcgag ccttcaccca
     2161 gcaccgtgcc catgaggacg acctgacagc tcccgtc
//
LOCUS       DATMZD010000283         4765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_77741,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000283 DATMZD010000000
VERSION     DATMZD010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4765
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77741"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1421
                     /locus_tag="VN478_05880"
     CDS             <1..1421
                     /locus_tag="VN478_05880"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWQ22237.1"
                     /translation="HDRHKTGDLMALVTNDLEAVREALGDGLLMISDFIIMTTFTLFA
                     MFRFNRSLTLMTLVPLVLIALVVTRSGPVVFRLFKRVQDAFSALSDYVQEAMTGIRVI
                     KVFTREEAAARGLRERALEIQRRNVRLVTLWGLTFPLIDFLGAIATLILLWAGGLLVT
                     RGQFSIGGLVAFNTYIGMLSWPMLALGWAINLFQRGQASLARINEILDMQPTILERPD
                     ALTLDAMRGEIEFDHVTFEREGKRILDDVSFHVVPGQRVGIIGPIGSGKTTIVSLLTR
                     AYDTTSGRVLIDGHDVRDYRIDDLRRAIGVVTQDVFLFSDSLEENINVTELPVGTEEV
                     HEATRLSGVYKNIMEFQDEFNTVVGERGVTLSGGEKQRVTIARAILKRPRILVLDDAL
                     SAVDADTEKHILDNIAGFETRPTTVVISNRISALRRMDKILVLDGGRVEEEGTHSDLL
                     RAKGMYWHIFRRQLVERKVEKE"
     gene            1418..3550
                     /locus_tag="VN478_05885"
     CDS             1418..3550
                     /locus_tag="VN478_05885"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWQ22238.1"
                     /translation="MSEPITQEEVKERSFDATLMRRLLHFARPQGWLLLLALVLVCVL
                     NAGDILRPLIVRNAIDQYVDPTSSRFVRLDSAEDASVVDGLVPVAARFALDGASYYDK
                     TELAHAGRILGRSLSGTQVSFYVITADKAAQLGTETMASYTGQTAPVLFKAPVPAFAE
                     GSYLMNVEDLQGLSAAERDRLRSPDRAGIRRLSLFFLVVVAVMLVVAYGNRLLLEVVG
                     QRVVYDIRVQLFDHIERLPFSYFDKAPSGRLTTRVTNDIAALADMYVNSLVNLAKDSL
                     LLVGVLVTMFMLNVFLTWILVGVLVAVIAITAILRIPMRDTHRMARTRLARINAFIAE
                     RLQGVRVVQLFAVEEKEKQKFDVINRDFTRVRLRLVHLNGIFRPLVAALTTFAIALLL
                     YFGGDRILGGTLSFGTLITFTFYAAIFFRPVQELAEQFDTIQSAMASAEKIFRVMDEP
                     EEVYTPEAFDGQVPASFDIEFQDVYLSYKEGEQVLKGISFRVEQGGSVALVGATGAGK
                     TSITGLVPRFYDVDSGHVLLGGMDVATLPAEFLRRSVAFVMQDVFIFSGSVLDNIALF
                     EEHPDEERANEIVDYLGMGFVKSLEGGLHYHLYERGNNLSSGQRQLISFARALYFDPK
                     VLILDEATSNIDTESEAVIQGAIRKILKGRTSIIVAHRLSTIRTVDRILVVQKGRISE
                     SGTHEELLARGGSYRSLYELQSLSEEKT"
     gene            3642..>4765
                     /locus_tag="VN478_05890"
     CDS             3642..>4765
                     /locus_tag="VN478_05890"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HWQ22239.1"
                     /translation="MSAVELVGLVLHGRYKIYEQAGTSGMATVCLAKQADTPDAVTAL
                     ILEPPITRDAGIVRRFLRSGEMGCHAAHPYVAPVEDYGQEQDLCFMVTRHEQLGVTLA
                     DLERRNGPLSMVQSAWVCSCIASALEGALAYGGIPFHGALRPTSVIITPSGDAQVACF
                     GTAPASGAPDQALGGSGALYAAPEQTEGRSVDSRTDLYALGVMLCEMLAQRIPTVSEV
                     RSFLAFEGTTRMEEFLRGIPDKLRPVLSGLLQWDPDERFSSPGEVIEALTGAGFPSPQ
                     RPQMESTGPGWERGPDLSSVSAPEPLLLQVPIVGMGDLYARETTGAVPLAADAERAGE
                     AAGSAEIPRPEEGFAWEIAAQPSSDPVAARAVASAPRRRWV"
BASE COUNT          851 a         1483 c         1492 g          939 t
ORIGIN      
        1 ttcacgacag acacaagacg ggcgatctca tggcgctcgt cacgaatgac ctcgaggcgg
       61 tccgtgaggc gctgggggac ggtctgctca tgatctcaga cttcatcatc atgacgacgt
      121 tcacactctt cgccatgttc cgcttcaacc gctcgctgac actcatgaca ctggtgcccc
      181 tggtgctcat tgcgcttgtc gtgacgcgtt cgggccccgt cgtgttccgg ctgttcaaac
      241 gtgtgcagga cgccttttcg gctctcagcg actatgtaca ggaggccatg accggcatcc
      301 gggtcatcaa ggttttcacg cgtgaggaag ctgctgctcg cggcctgcgc gagcgagccc
      361 tggagatcca gcgccgcaat gtccgcctcg tgacgctctg gggactcacg tttccgctca
      421 tcgacttcct tggcgccatc gccacgctga tcctcctgtg ggcaggcggt ctgctcgtca
      481 cgagagggca gttcagcatc ggaggactcg tggcgttcaa cacgtacatc ggcatgctca
      541 gttggccgat gcttgcgctg ggctgggcca tcaacctgtt ccagcggggt caggcgtctc
      601 tcgcacggat caacgagatc ctcgacatgc agccgacaat cctcgaacgg cccgatgccc
      661 tcacgctcga cgccatgcgc ggtgagatcg agttcgacca cgtgacgttc gaacgcgaag
      721 gcaagcgcat tcttgatgac gtcagcttcc atgtggttcc tggtcagagg gtcggcatca
      781 tcggccccat cggctcaggc aagacgacga tcgtctcgtt gctgacccgc gcttatgata
      841 cgacgtcggg acgggtcttg atcgatgggc atgacgtgcg cgactatcgg atcgacgacc
      901 tgcgccgggc aatcggggtc gtgactcagg atgtgttcct gttctcggat tcactcgagg
      961 aaaacatcaa cgtgacggag ctgccggtcg gcaccgaaga ggtccacgaa gcgacaaggc
     1021 tgtccggtgt ctacaagaac atcatggagt tccaggacga gttcaacacg gtcgtggggg
     1081 agcgcggcgt cacactctcc ggcggcgaga agcagcgtgt cacgatcgca cgcgccatcc
     1141 tcaagaggcc tcgcatcctc gtgctggacg acgccctgtc cgcggtggac gccgacaccg
     1201 agaagcatat cctcgacaac atcgcaggct tcgagaccag gccgacgacg gtcgtcatca
     1261 gcaaccgcat ctcggcactc cggcgcatgg acaagatact ggtcctggac ggtggacgcg
     1321 tcgaggaaga ggggacccac agcgacctgc tgcgggcgaa gggcatgtac tggcatatct
     1381 tcaggagaca gctggtggag cgcaaggtgg agaaagaatg agcgagccga taacgcagga
     1441 agaagtgaag gagcgttcat tcgacgcgac gctgatgcgt cgcctgctgc acttcgccag
     1501 gccacagggc tggctgcttc tgctcgcact cgtgctcgtg tgtgtcctga acgccggaga
     1561 catcctgaga cccctgatcg tgcgtaacgc catcgaccag tatgtcgatc cgacttcctc
     1621 gaggttcgtc cgactggaca gcgccgagga tgccagcgtc gtcgacggtc ttgtgccggt
     1681 ggctgcccgg tttgccctgg atggcgcctc ctactacgac aagactgagc ttgcgcatgc
     1741 gggccgcatc ctcggccgct ccctctcggg gacccaggtc tcgttctatg tcatcacggc
     1801 ggacaaggcg gcacagctcg ggaccgagac catggcttcg tacacgggtc agacggcgcc
     1861 agtgctgttc aaggcgccgg ttcccgcttt cgcagaaggg tcgtacctga tgaacgtgga
     1921 ggacctccag ggcctgtccg ccgccgagcg tgaccgactc cgctcgcccg acagggccgg
     1981 tatcaggcgc ctgtcgctct tcttccttgt cgtcgtcgcg gtgatgctgg tcgtcgcata
     2041 cggcaacagg cttcttctgg aggtcgtggg gcagcgggtc gtctacgaca tccgtgtgca
     2101 gctctttgat cacatcgagc gtctgccgtt cagctacttc gacaaggctc catccggtcg
     2161 actcaccaca cgggtcacca acgacatagc cgctctcgcc gacatgtacg tcaactcgct
     2221 ggtcaatctt gccaaggatt cgctcctgct ggtgggtgta ctcgtcacga tgttcatgct
     2281 gaacgtgttc ctgacgtgga tcctggtggg agtgctggtg gcagtcattg ccatcaccgc
     2341 gatccttcgc atccccatgc gcgacactca ccgcatggcc cggacgcgcc tggcacgcat
     2401 caacgcgttc atcgcggagc gtctgcaggg tgtacgagtc gtgcagctgt ttgccgtcga
     2461 ggagaaggag aagcagaagt tcgacgtcat caatcgcgac ttcacccgcg tccgcctgcg
     2521 cctggtccat ctcaacggca tcttccgacc gctcgtggcg gctctcacga cgtttgccat
     2581 cgcccttctc ctctacttcg ggggagaccg catcctcggc gggaccctgt cgtttgggac
     2641 gctcatcacg tttacgttct atgccgcgat cttcttccgc cccgtgcagg aacttgcaga
     2701 gcagttcgac accatccagt cggccatggc ttcggctgag aagatcttcc gcgtgatgga
     2761 cgagccggag gaggtctaca cccctgaggc attcgacggt caggtccctg cttccttcga
     2821 tatcgagttt caggacgtct atttgagcta caaggagggc gagcaggtcc tcaagggcat
     2881 cagcttcaga gtcgagcaag gagggtctgt ggctctcgtc ggcgccaccg gcgccgggaa
     2941 gacgtccatc acgggactgg tcccgagatt ctatgatgtc gacagcggac atgtcctgct
     3001 cgggggcatg gatgtcgcga ctctgcctgc cgaattcctc aggcgtagcg tcgcctttgt
     3061 catgcaggac gtgttcatct tcagcggcag cgttctggac aatattgcgc tcttcgaaga
     3121 acaccccgac gaagaacgtg cgaacgagat cgtcgactac ctgggcatgg ggttcgtgaa
     3181 gagccttgag gggggactgc actaccatct gtatgagcgt ggcaacaacc tctcatcagg
     3241 ccagcggcag ctcatctcgt ttgctcgggc gctctacttc gaccccaagg tgctcatcct
     3301 cgacgaggcc acgtccaata tcgacactga gtcggaagct gtgatccagg gggccatacg
     3361 caagatcctc aaggggcgta cgagcatcat cgtcgcgcac cgcctgtcga ccattcgtac
     3421 cgtagaccgc atcctcgtcg tacagaaggg acgtatcagc gaatccggga ctcacgagga
     3481 actgctggcc aggggcggca gctacaggag cctctacgaa ctccagtccc tgagcgaaga
     3541 gaagacctga cgcttcgttt ccccctgcta cagcgattga cagggcagac catggcgcta
     3601 tacatgcatg gggtggcatc gtcgtgccgc cggccggaga ggtgagcgca gtggaactcg
     3661 tggggctcgt tctgcatgga cggtacaaga tctacgagca ggccggaacg tcgggcatgg
     3721 ccactgtctg cctcgcgaag caggctgaca cgcccgacgc cgtcacggcc ctcattcttg
     3781 agccgcccat tacacgtgat gccggcatcg tccggcgctt tctcaggtcc ggtgaaatgg
     3841 gctgtcatgc cgcgcacccg tatgtagctc ccgtcgaaga ctacggccag gaacaggacc
     3901 tgtgcttcat ggtgacccgc catgaacagt tgggggtcac gctcgccgac ctggagcgga
     3961 ggaacgggcc cctgtcgatg gtccagtctg catgggtctg ctcatgtatc gcctcggcgc
     4021 tggagggggc cctcgcgtac ggcggcatcc cattccacgg agcgcttcgc ccgacgagtg
     4081 tcatcatcac gccgtctggt gatgcccagg tcgcctgctt cgggactgcc cccgcgtcgg
     4141 gtgctcctga tcaggctctc ggggggagtg gcgctctgta tgcggctcct gagcagaccg
     4201 agggccgcag cgtggacagt cgaaccgatc tgtatgcgct gggcgtgatg ctgtgcgaga
     4261 tgcttgcgca gcgcattcca acagtgtcgg aggtccgttc attcctggca ttcgagggga
     4321 cgacccgcat ggaggagttc ctgaggggca tacctgacaa gctgcgccct gtgctgtccg
     4381 gcctgctgca atgggatccc gacgagcggt tttcgtctcc aggcgaggtc atcgaagcgc
     4441 tgacaggagc aggattcccc tcgccgcagc gaccccagat ggaatcgacg ggacccgggt
     4501 gggagcgtgg tccagatctc agctccgtct cagctcctga gccgctcctg cttcaggtgc
     4561 ccatcgtcgg catgggcgac ctgtacgcac gggagacgac gggagcagtc cctctggccg
     4621 cagacgctga gcgtgcaggt gaagccgccg gcagcgccga gatccctcgc cccgaagagg
     4681 ggtttgcctg ggagatcgcg gcccagccgt cgagcgaccc ggtcgccgcg cgggcagttg
     4741 cctcggcgcc gcgccgtcga tgggt
//
LOCUS       DATMZD010000284         3649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_8495,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000284 DATMZD010000000
VERSION     DATMZD010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8495"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="VN478_05895"
     CDS             complement(<1..529)
                     /locus_tag="VN478_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HWQ22240.1"
                     /translation="MFTGCKKATPDDGTGAKFHIGVVTGTVSQSEDDLRGGERLVELY
                     GNSADGGMITTVTYPDNFTSEQETTIAQIVGLADDPLMKAIVVNQAVPGTAEAFKRVR
                     EKNPDILLFAGEPHEDPNVIEAAADQAVSADNVARGYLIIWAAKQMGAKTFVHISFPR
                     HMSYELLSRRRYIMEE"
     gene            complement(777..2393)
                     /gene="groL"
                     /locus_tag="VN478_05900"
     CDS             complement(777..2393)
                     /gene="groL"
                     /locus_tag="VN478_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453748.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HWQ22241.1"
                     /translation="MDAKQIIFNDEAYEKIRAGVDRLANTVKVTLGPKGRHVALEKKF
                     GSPVLSDDGVSIAKEIELADPNENIGAQLVREIAQKTEDQAGDGTTTATVLAQIMIQE
                     GIKNVTAGADSVALKAGMDKATTAVVDELKKLSRQVKTREEKAQVATVSSKMPAVGEA
                     IADAMEKVGKDGVISVEESQGLEMKVETVEGMQFDRGYISPYLVTDPDRMEVVLKNPL
                     IFITDKKVTSIQEFLPVLEKLSQKGRPFLLVADDITGEALATIVLNKVRGTFSCVAVK
                     APGFGDRRKAMLEDIAVLTGGKVLSEDLGLTFEKVTEDMFGSADEVKVDKDNTTIVGG
                     KGAKEEIKSRIGQIRKQVEETKSDYDKEKLQERLAKLAGGVAIIKVGAPTETAMKELK
                     HRVEDAVNATKAAVAEGIIIGGGAALVKAGRAIDGLKLTGDEKTGAEIIRKSLREPLR
                     IIADNSGYEGVIAVQKVLEGDDDLGFNSLNNKFEDLFKSGIVDPLKVTRLALLNAESI
                     ASLLLSTAAMVTTVPKAESSAPAAPAYPEY"
     gene            complement(2406..2696)
                     /gene="groES"
                     /locus_tag="VN478_05905"
     CDS             complement(2406..2696)
                     /gene="groES"
                     /locus_tag="VN478_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HWQ22242.1"
                     /translation="MAKLVPIGDHVVIKVETVKETMKSGIVLPDSAKEKPQQGTVMAV
                     GSGVMLDNGTRVPLEVKEGDKVVYSKYSGNEIKLDEEEYVVLSQHDILAIVK"
     gene            2932..3423
                     /gene="tsaE"
                     /locus_tag="VN478_05910"
     CDS             2932..3423
                     /gene="tsaE"
                     /locus_tag="VN478_05910"
                     /inference="COORDINATES: protein motif:HMM:NF014426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HWQ22243.1"
                     /translation="MSRCRQSTEGIGILLGSCLLETIAEGRRDSVVVGLVGPLGAGKT
                     VLTAALSAALGVDRATVASPTFVLERVYLGRQPVRHFDLYRIEGPGQLVEMGFEEELE
                     KPGVTVIEWAERSGGLLDLYERIEVRFEVLGDEVRRIVVRDFYQPSIVAGCFSVETGS
                     ASR"
     gene            3395..>3649
                     /locus_tag="VN478_05915"
     CDS             3395..>3649
                     /locus_tag="VN478_05915"
                     /inference="COORDINATES: protein motif:HMM:NF013012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22244.1"
                     /translation="MSRQALLLVNTATILGVVGVARGDRVLAEESLRLATASNTVLSA
                     VSHVLMESGCTPDDLQTVILVRGPGTFTGSRVGASIAKALA"
BASE COUNT          682 a         1032 c         1050 g          885 t
ORIGIN      
        1 cctcttccat gatatagcgt ctgcggctca gcagctcata gctcatgtga cgagggaatg
       61 agatatgaac gaacgtcttg gcgcccatct gcttggcagc ccagatgatg aggtagccac
      121 gggcgacgtt gtcggcgctg acagcctggt cggctgcggc ctcgatgacg tttggatctt
      181 cgtgtggttc gccggcaaac agaaggatgt ccggattctt ctcacgcaca cgtttgaacg
      241 cttcagcagt gcccgggaca gcctggttga cgacaatggc tttcatgagc gggtcgtcag
      301 caaggcctac aatctgggca atggttgtct cctgctcgga cgtgaagttg tccggatagg
      361 tgaccgtcgt gatcatgcca ccgtctgccg aattgccgta cagctctacg aggcgttcgc
      421 cgccgcggag gtcgtcttct gactgggaaa ctgtgccggt tacgacgccg atgtgaaact
      481 tggcacccgt accgtcatca ggtgttgcct tcttgcagcc cgtgaacatg actgcaacaa
      541 gcatgaatga cagcagtact gttagcagct tcttcactct cttcctcccc taaagaatta
      601 gccatagttc agcctgacca catatttagc acagtcacgg cttttttcaa ggtatcttca
      661 catgtttacg gagcagcagc ttgcatctgg actgagtggc ttgcatcgca agatgtgcag
      721 aaaccccggc cgtgaggccg gggtttctgc ggaaggacgc agtcgcgaga tcgcgtctag
      781 tactctgggt aggcgggagc cgcgggtgca gagctctctg cctttggaac ggtcgtgacc
      841 atggccgccg tcgagaggag caggcttgca atgctctctg cattcagcag cgcgaggcgt
      901 gtgaccttca gcggatcgac gataccactc ttgaagaggt cctcgaactt gttgttgaga
      961 gagttgaacc cgagatcgtc atcgccctcg aggactttct gcaccgcaat gactccctca
     1021 tatccggagt tgtcagcgat gatcctcagt ggctcacgga gggacttccg aatgatctcg
     1081 gcaccggtct tctcgtcgcc ggtcaacttg aggccatcga tggcccgtcc cgccttgacc
     1141 agggctgcac cgccgccaat gatgatgcct tccgcgacgg ctgccttggt agcattgacg
     1201 gcatcctcga ctctgtgctt gagttccttc atggctgtct cagtcggtgc gccgaccttg
     1261 atgatggcaa cgccaccagc aagcttcgcc aggcgctcct gaagcttttc cttgtcgtag
     1321 tccgacttgg tctcttcgac ctgcttgcga atctggccga tgcgggactt gatctcttcc
     1381 ttggctccct tgccaccgac gatggtcgtg ttgtccttgt cgaccttgac ttcgtcagca
     1441 gagccaaaca tgtcttccgt gaccttctcg aacgtgagac cgagatcttc gctgagaacc
     1501 ttgccaccag tcaggacagc aatatcctcg agcatggcct tgcgcctgtc gccgaagcca
     1561 ggagccttga cggcaacgca tgagaacgta ccgcgcacct tgttgaggac gattgtcgcc
     1621 agcgcttcgc ccgtgatgtc atcggcgacg agcaggaatg ggcgtccctt ctgagagagt
     1681 ttctccagca cgggcaggaa ctcctggata gacgtgacct tcttgtccgt gatgaagatc
     1741 agaggattct tcagcacgac ttccatgcgg tcggggtcag tcacgagata cggtgagatg
     1801 tagccacggt cgaactgcat gccctcaacg gtctcaacct tcatctcaag gccctgggac
     1861 tcctcgacgc taatgacgcc atccttgcca accttctcca tggcgtccgc aattgcctcg
     1921 ccgacggcgg gcatcttgga tgagacagtg gcgacctggg ccttctcctc acgcgtcttg
     1981 acctgacggg acagcttctt gagctcgtcc acgacagcgg tcgtcgcttt gtccatgcca
     2041 gccttgagag caacggaatc tgcgccggcg gtgacattct tgatgccttc ctggatcatg
     2101 atctgagcaa ggaccgtagc ggtggtcgta ccgtcaccgg cctggtcctc agtcttctgg
     2161 gctatttccc tgacgagctg ggcaccgatg ttctcatttg ggtccgccag ttcgatttcc
     2221 ttggcgatgc tgacgccatc gtccgacagg actggcgaac cgaacttctt ctcaagtgca
     2281 acgtggcggc cctttggtcc tagcgtaacc ttgacggtgt tggcgagtct gtcgacgccg
     2341 gcacgaatct tctcatatgc ttcatcattg aagatgatct gtttcgcatc catagtcatt
     2401 ccctcctact tgacgatagc gaggatatcg tgctgagaca gcacgacgta ctcttcttcg
     2461 tcgagtttga tttcgttgcc gctgtacttg gaatagacaa ccttgtcgcc ttccttgact
     2521 tcaaggggaa ccctggtgcc gttgtccagc atgacgccgg agccaactgc catcaccgtg
     2581 ccctgctgag gcttctcctt ggcgctgtca ggaaggacaa tgccactctt catggtctct
     2641 ttgacggtct ccaccttgat gactacatga tcaccgattg gtacaagctt tgccatattg
     2701 tcgtacctcc tgtactagag attctcaatg gattagcact ctctgtgcat gagtgctaag
     2761 tcattatagg agtgcatgat gagctgtcaa gtgtcgcaga agcctgcagt cgtgcgctct
     2821 gaactcaagc cgagtgaggg gttgggttcc aggcactgtt gcacaaggac gggcttcttg
     2881 ctaagattag ttcccgacat gatatacgaa acttgcatag aggttccatt cgtgagtcgc
     2941 tgccgtcagt ccaccgaggg catcggcatc ctgcttggca gttgtcttct ggaaacgatt
     3001 gcggagggtc gtcgcgactc agttgttgtc gggttggtgg gacccctggg tgcgggcaag
     3061 acagttctta cggctgctct ctctgcggct ctcggcgtgg atcgagcaac tgtggcaagc
     3121 cccacgttcg ttcttgagag ggtatatctg ggcaggcaac cggtgaggca ctttgacctc
     3181 tatcgcatcg agggtcccgg ccagctcgtg gaaatgggct ttgaggagga gctggagaag
     3241 cccggcgtga ccgtcatcga gtgggcagaa cggtccggcg gactcctcga tctgtatgag
     3301 cgtatcgagg tccgctttga agtcctgggt gacgaagtca ggcgcatcgt tgtgcgcgat
     3361 ttctaccagc catccatcgt ggcggggtgt ttcagtgtcg agacaggctc tgcttctcgt
     3421 taacacggca accatcctgg gtgtcgtcgg cgtggcgcga ggcgaccggg ttctggcaga
     3481 ggaatcactg cggttggcga cagcgtccaa cactgttctg tcggctgtga gtcacgttct
     3541 catggagtcg ggatgcactc ctgacgacct gcaaacggtg atactcgtca ggggccccgg
     3601 aaccttcaca gggtcacgcg ttggcgcaag tattgccaag gcgcttgcc
//
LOCUS       DATMZD010000285         2165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_48228,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000285 DATMZD010000000
VERSION     DATMZD010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48228"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1516
                     /locus_tag="VN478_05920"
     CDS             <1..1516
                     /locus_tag="VN478_05920"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HWQ22245.1"
                     /translation="HIESAVDVVYQITGVRQQETVPDHVLELAGEIEVVDVSPEALQQ
                     RLTEGKIYIPDKAKQAMHHFFRRGNLLALRELALRYAARRVDKDMRHYMQRKTITGPW
                     HAGTRIMTCVRPDGNAERLVRVAHGMAADLDAPWYVVYVESPQTGQAGGPGRDALARS
                     FNLAEELGGKVVILPATRITDTVLEFAHDKNITLIIVGLSHRSRWLEFTQGSIAADIV
                     REAGSIHVLVVGDTDKAEGPARSHETRAAQRKWASITWPVALVGCAAAICALLRRYVG
                     PSDVPLVMLLPVVFSSVLWGLAAGLLSSATAIVAFDLFFVPPYFSFAIGDLKQYAPTF
                     LIFIIVGVVTSLLEDTILRQETRGRQRERFVSTVYEFSHDLMAGRSLEENLNQATRDI
                     ADAFNCDVVILMPDSRSVLVPRASIGNLQTLDDNAVGVATWTLNQRQPAGQGTETLSH
                     IDWSFYPLVAYERGIGVLGIQLHGESPAAPLEQRQLIDAFANILALFVARNVSS"
     gene            complement(1545..1922)
                     /locus_tag="VN478_05925"
     CDS             complement(1545..1922)
                     /locus_tag="VN478_05925"
                     /inference="COORDINATES: protein motif:HMM:NF014119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HWQ22246.1"
                     /translation="MGGQHNRALGNAGEELAAQWLTDHGFHIVARNVRTVYGELDVVA
                     EKSGHIHVVEVKTRKGTGYGTPLEAIDRRKQEHLRRSTMAALSAGLPGLTRPVRGIHI
                     DAMSILTGSGAAPCIEYLEDILA"
     gene            complement(1882..>2165)
                     /locus_tag="VN478_05930"
     CDS             complement(1882..>2165)
                     /locus_tag="VN478_05930"
                     /inference="COORDINATES: protein motif:HMM:NF013514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22247.1"
                     /translation="MAIIDADATILSVAGASIAAKVSRDAAMRVMARHVHGYGFEGNV
                     GYGTREHIEALRTRGPSSEHRRTFVVKQLVETEELPLGRTTQSGAGKRR"
BASE COUNT          433 a          632 c          605 g          495 t
ORIGIN      
        1 gcacatcgag agtgctgtcg atgttgttta ccagattacg ggcgttcgcc agcaggagac
       61 agtacccgat catgtcctcg aactggcagg cgagatcgag gtggtcgatg tctcgcccga
      121 agcgttgcag caacgactga ctgaaggaaa gatctacatc cccgacaagg caaaacaggc
      181 aatgcaccac ttctttcgta gagggaatct attggcgctg cgagagcttg cgttgcgcta
      241 tgctgcccga cgtgtcgaca aggacatgcg ccactacatg caacgaaaga caattactgg
      301 gccatggcac gctggtactc ggatcatgac gtgtgtgaga cctgacggaa atgcagaaag
      361 acttgtacgc gtcgcgcatg ggatggcagc agatctcgac gctccgtggt atgtcgtcta
      421 tgtcgagtct cctcagacgg gtcaggcagg cggccccggc cgtgatgcac ttgcacgttc
      481 cttcaatctt gccgaggagc tgggcggcaa ggtcgtgatc ctgccggcaa ctcgcatcac
      541 ggatacggtg ctcgaattcg cccacgacaa gaacatcacc ttgatcattg tcggtctctc
      601 ccaccggtca cgctggctcg aattcacaca aggctccatc gcagccgata ttgtgcgaga
      661 agcgggttcc attcatgttc ttgtcgtggg ggacactgac aaagcagagg gaccggctcg
      721 atcacatgaa acgcgggcag cgcaacgcaa gtgggcatcg atcacctggc cggtcgctct
      781 cgttggatgt gcagcagcga tctgtgctct tctgcgccga tatgtcggtc cttctgatgt
      841 tccacttgtc atgcttcttc cagtggtgtt cagcagcgtc ttgtgggggc ttgccgcagg
      901 acttctttcg tcagccacgg ccattgtcgc ttttgatctc ttctttgtgc caccgtattt
      961 ctcgtttgcg atcggcgatc tgaagcagta tgcaccgacg ttccttatct ttatcatagt
     1021 tggtgtcgta acaagtctgc ttgaagatac tatccttagg caggaaacga gggggagaca
     1081 gcgagaacga tttgtctcta cggtctacga gttcagccat gatctcatgg caggacgttc
     1141 gctggaagag aacctcaatc aggcaacacg cgacattgcc gatgcattca actgtgatgt
     1201 cgtgatcctc atgccagact ctcgcagtgt cctggtgcct cgtgccagca tcggaaacct
     1261 gcagacgctc gacgacaatg cggtcggcgt ggcaacgtgg actctcaacc agcgacagcc
     1321 ggccggacaa gggacggaaa cgctgtctca catcgactgg tcattctatc cccttgttgc
     1381 ctacgaacgg ggcatcggcg tcctcggtat ccagctgcac ggtgaatctc cagccgcccc
     1441 acttgaacag cgtcaactca tcgacgcctt cgcaaacatc ctggcgcttt ttgtcgcaag
     1501 gaatgtctct tcctaggacc tccttccttg tcctggagcc tgctctatgc gagaatgtcc
     1561 tccaggtact cgatacatgg cgcggccccg ctgcccgtga ggatactcat tgcgtcgatg
     1621 tggatgcccc gtacgggacg tgtcaggccg ggaagccctg ctgacagagc ggccatcgtc
     1681 gaccgtctca gatgttcctg cttgcgacgg tcgatcgcct cgagaggagt cccatatcca
     1741 gttcccttcc tggtcttgac ttcgacaacg tggatgtgac cactcttttc ggcgacgaca
     1801 tccaattctc cgtagacggt acgaacattc ctggccacaa tgtgaaagcc gtgatcagtc
     1861 agccattggg ctgccagttc ctcaccggcg tttcccagcg cccgattgtg ttgtccgccc
     1921 aagtggcagc tcctcagtct ctacaagttg cttgacaacg aacgtccgtc tgtgttcgct
     1981 ggaggggccc ctggtccgga gagcctcgat atgctcgcgt gtgccatagc cgacattgcc
     2041 ctcgaatccg tatccgtgca cgtgtcgagc catgacgcgc atggcggcat cccgtgagac
     2101 cttggcggcg atgctggcac ccgcaacgga caggatggtc gcatccgcgt caatgatcgc
     2161 catgg
//
LOCUS       DATMZD010000286         2121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_58960,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000286 DATMZD010000000
VERSION     DATMZD010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58960"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="VN478_05935"
     CDS             complement(<1..721)
                     /locus_tag="VN478_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011160262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWQ22248.1"
                     /translation="MISFSNVNKQYGKQLIFVEASYQLNPGEKVGLVGPNGSGKTTLF
                     RMIVGEEFPDEGEVSIPKKLTIGYFRQDVEEMKGRSVLDEAIAGSGRVGDLHHELEAL
                     NHALGDPEQMDDMDRILARFGEVQEEYEHLGGYTIEAQAREVLNGLGFQDEQIDGDVG
                     ALSGGWKMRVALARVLLGKPDILLMDEPTNHLDIESIIWLEQFLKSLPSTLLMTSHDR
                     EFMNRIVTKIVEIDAGEIVSYS"
     gene            1012..1503
                     /locus_tag="VN478_05940"
     CDS             1012..1503
                     /locus_tag="VN478_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22249.1"
                     /translation="MFLFTTLKFGWRAVAGAMAAEFVLRIATTGFCGAFTQSLRYVQP
                     AWQGGMVTLLVLPLTMQLLELGLHLLRGTPNLRVGVGVSTGFTILSTLFHVYATRQGA
                     YLTGAHATSLGDDLRRTPRLLAGFLALRWCALDKTEKDGNVSADSPVLHQAARPRRPS
                     CSR"
     gene            1550..>2121
                     /locus_tag="VN478_05945"
     CDS             1550..>2121
                     /locus_tag="VN478_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22250.1"
                     /translation="MNRTKIDLHWREPKAAARYSHGVALHSHTMHSRETLSFIPRHVP
                     RIPVLGSLVAAQERRYRERSGKDIDYSRAWWTPPLSGPQALELESKQVTGQLGLEALV
                     SLTDHDNMDAPRLLTVVESSRHVPHSVEWTVPFGPSFVHLGVHNIPRAPLASIEAGMR
                     EYTQQPDPALLAGLLEELSSSRDTLVVLNHP"
BASE COUNT          373 a          650 c          668 g          430 t
ORIGIN      
        1 ccgagtagga cacgatctca ccggcgtcga tctcgacgat cttggtgacg atgcggttca
       61 tgaactcgcg gtcgtgcgag gtcatcagca gggtgctggg tagcgatttg aggaactgct
      121 ccagccagat gatcgactcg atgtccaggt ggttggtcgg ctcgtccatc agcaggatgt
      181 cgggcttgcc caacagcacg cgggccagcg cgacacgcat cttccagccg ccggaaaggg
      241 ctccgacgtc cccgtcgatc tgttcgtcct ggaagcccag gccattcagg acttcgcggg
      301 cctgcgcctc gatcgtgtac ccgcccaggt gctcgtattc ctcctggacc tcgccgaaac
      361 gggccagaat gcggtccatg tcgtccatct gttcgggatc gcccagggca tggttgagcg
      421 cctccagttc atggtggagg tcaccgacac gcccgctgcc cgcgattgcc tcgtcaagga
      481 ccgatcgccc cttcatctcc tcgacgtcct gccggaagta gccgatggtc agtttcttgg
      541 ggattgagac ctccccttcg tcggggaact cctcgccaac gatcatccga aagagcgtgg
      601 tcttgccgga gccgttcggg cccaccagac ccaccttctc gcccggattg agctgatagg
      661 aggcttcaac gaagatgagt tgcttgccgt actgtttatt gacgttggag aaagagatca
      721 tcgggaacca gacaaggatg aacgccgccg gacggcggtg tcgcacgttg aggcagtctc
      781 cttccagtgt acaaagcggc gcgcctcggc cgtgagcgtc ttcaacaaaa ttggcgcaaa
      841 actgtcatcc ggttggaagg gagtgccctg tagcctgatg gcttgccgtc gcgggaacag
      901 tccgaaagct tggtttgcgc gctgcgacga atactcgccc agccggccgc ggtgctcttc
      961 cactcctggc actggaaggc ggccctgttc agtgcctccc tgcgggctct gatgttcttg
     1021 ttcacgacgc tgaagttcgg ttggcgcgcc gtggccggcg cgatggcggc cgagttcgta
     1081 ctgcgcatcg caaccacggg cttttgcgga gcattcacgc aatcgttgcg gtatgttcaa
     1141 ccggcgtggc aaggaggcat ggtgacgctg cttgtgctgc cgctgacgat gcagttgctg
     1201 gaactgggac tgcatctgct gcgcggcacg ccgaacctgc gcgtcggtgt cggtgtgtcc
     1261 acggggttca ccattctgtc aacgctcttc cacgtgtatg caacgcggca gggcgcgtac
     1321 ctcacagggg cccacgcgac ctcgctggga gacgacttgc ggcgcacgcc tcggctgctc
     1381 gcaggctttc tggccttgcg atggtgcgca ctcgataaaa cagaaaaaga tgggaatgtt
     1441 tcagcagatt caccggttct ccatcaagct gcaaggcccc gtcggccatc ctgttccaga
     1501 tgaatgcgtg gcgtgacccg tcgattctca ggagcgtcag gaggcaaaga tgaaccgtac
     1561 gaagattgat ctgcactggc gggagccgaa ggccgccgcg cgatacagtc atggtgtcgc
     1621 tctgcacagc catacaatgc actcgcggga gacactgtcg ttcattcccc gtcatgttcc
     1681 gcgcatcccg gtgctgggct cgctggtggc ggcgcaggaa cggcgatacc gtgagcgcag
     1741 cggcaaggac atcgactact cgcgggcatg gtggacgccg ccgttgagtg gaccacaggc
     1801 cctggaactg gagtcaaagc aggtgaccgg gcaactcggc ctggaggctc ttgtctctct
     1861 caccgaccac gacaacatgg acgctccgag gttgctcacg gtggtggaat cgtcgcgtca
     1921 tgtacctcac tcggtggagt ggaccgtgcc ctttggcccg tcgttcgtcc acctgggcgt
     1981 tcacaacatt ccgcgcgccc cgctggcgtc gattgaggcc gggatgcgcg agtatacgca
     2041 gcaacccgat cccgccctgc tggccggtct gctggaggaa ctcagctcgt cgcgtgacac
     2101 gctggtggtg ctgaatcatc c
//
LOCUS       DATMZD010000287         3354 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_77799,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000287 DATMZD010000000
VERSION     DATMZD010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77799"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..380)
                     /locus_tag="VN478_05950"
     CDS             complement(15..380)
                     /locus_tag="VN478_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWQ22251.1"
                     /translation="MARPIHFELTAIDPARAITFYRTVFGWTIEPAGGPVEYYLITTG
                     PTTEPGINGAITRTRDGTVGTVNTISVASVEESVVAVTANGGTDLMTPQPMPGVGRLA
                     MCKDTEGNVFGILQMEARP"
     gene            638..2029
                     /locus_tag="VN478_05955"
     CDS             638..2029
                     /locus_tag="VN478_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWQ22252.1"
                     /translation="MDVVTGRPGDLPENREGRQPRKRLTGFRAFLVVWLGQVLSMTGS
                     SMSQFALSIWAYQKTGLATSLAMVEFFFQGAMIAATPLAGVYVDRWNRKLTMMLSDLL
                     AVCGTIAILILYHAGQLAIWHLCVVGVVNGIGQAFQFPAYSAAITQMIPKEHYARAGG
                     LMSLAESGSVILAPIFAGLLIGIIKVGGILTIDVVTFGFAFGALLLIDVPSPAREVKQ
                     RESMWRQMTFGFRYIALRRPLLYLLTMYAVTNFVMSIGMPLMTPMVLARTANDAKALA
                     MVQSAAGIGGVLGGLLMGIWGGPRRRILGILFGNAMNFCVLGLTIALGRSPVWWAAGF
                     VVGQCLGGMVNGLSQSIWQVKVPHEYQGRVFASRRLLASIISPAALLISGPLADKVFE
                     PAMAPSGWMARAFGRFTGTGPGTGMALIIFISCMLAMLTSLTGFAVPGIRHLETLIPD
                     NDAVPAQEPVPGD"
     gene            complement(2043..>3354)
                     /locus_tag="VN478_05960"
     CDS             complement(2043..>3354)
                     /locus_tag="VN478_05960"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWQ22253.1"
                     /translation="WDTMTIRVVANQSDPRTLVFRVSPLADEKGVMTKVLLTGLDITD
                     QERELEQRSRAEKLESIGLLAGGVAHDFNNLLSGILGRLSLARADRDHPDAVQQHIAQ
                     AETGALSARDLARQLLTFDKGWQPIRSRCDIRPLIRNAVGLASSGSAIEFEENISPEL
                     ADIRADRGQMSQVLMNLLLNAKQAMDGVGHIWITAENREVSREQPWGTLPDGPYVSVS
                     ITDEGHGIDPAALSRLFEPYFTTKQTGTGLGLATCYSIIAKHGGIIEAFSVPGRKGAT
                     FDFGLPADLSGPHVVAAADSTGAQRNLVKGSGRVLIVDDETIIRTTTTQLLEHLGYKA
                     LAVPDGDSGLTAVREGMAAGEPFTAALLDLTIPGRKSGYDIIAELKQLDPGIVAIASS
                     GSVASLPDRYISRGFDASLEKPYTMQQLSDLLSRMLQGKETRPR"
BASE COUNT          528 a         1030 c         1114 g          682 t
ORIGIN      
        1 ccgcgtgatg cggttcaggg tctagcctcc atctgcagga tgccgaagac gttcccctcc
       61 gtgtccttgc acatggccag cctgccaaca ccgggcatgg gctgaggcgt catgaggtcg
      121 gtgccaccat tggccgtcac cgcaacgacg ctctcctcta ccgaggcgac gctgatggtg
      181 ttcacagtgc cgacggtgcc gtcgcgcgtg cgggtgatcg ccccgttgat gccgggctcc
      241 gtggtagggc cggtggtgat gagatagtac tccacgggtc cgcctgccgg ttcgatcgtc
      301 cagccgaata ccgtcctgta gaaggtgatg gcgcgagcgg gatcgatggc ggtgagctca
      361 aagtgaatgg gtcgagccat agtgagcctc ctgatggatg tgcgaccgct gcggtctctg
      421 ccgacagtgt accgcgtgca gcgggtctgt ccacgtggcg gcttttgaca ttgcgcgacg
      481 gtctcggtat gctttcaacc actgtgcgtg cacaaggagc ctgcatgagg acctcgacat
      541 cgaacaatgt ggacagtggc gtctggcaca cgatcctgga ccatccacgc gttcccgcgg
      601 acaggtcaga cctgctgcgg ttggcgagga ctgagccgtg gacgtcgtga ccggccggcc
      661 aggggatctg ccggagaacc gcgaggggcg ccagccgcgc aagcggttga ccggctttcg
      721 cgcgtttctg gtcgtgtggc tgggtcaggt cctgtccatg accgggtcct ccatgagcca
      781 gtttgcgctg agcatctggg cctatcagaa gacgggcctg gcgacttcgc tcgcaatggt
      841 ggagttcttc ttccaggggg ccatgatcgc ggccaccccg ctcgcgggcg tctacgtcga
      901 ccgctggaac cgcaagctta cgatgatgct ctcggacctg ctggcggtct gcggcacgat
      961 cgcgatcctg atcctgtacc acgccggaca gctcgcgatc tggcacctgt gcgtcgtggg
     1021 cgtcgtcaat ggtatcggcc aggcgttcca gttccccgcg tactcggccg ccatcacaca
     1081 gatgatcccc aaggaacact acgcccgcgc aggaggactg atgtcccttg ccgagagtgg
     1141 ctcggtcatc cttgcgccga tcttcgccgg cttgctcatc ggcatcatca aggtcggggg
     1201 catcctgacg atcgacgtcg tcacgtttgg atttgccttc ggcgcactgc tcctgatcga
     1261 tgtgccgtcg cccgctcgag aagtgaagca gcgcgagtcc atgtggcgac agatgacctt
     1321 tggtttccgc tacatcgcgt tgcggcgccc ccttctgtat ctgctgacca tgtatgcggt
     1381 caccaacttc gtcatgagca tcggtatgcc cttgatgacg cccatggttt tggcccgcac
     1441 cgccaatgac gccaaggcac tggccatggt gcagtccgcg gccggcatcg gaggcgttct
     1501 cggcggcctg ctcatgggca tctggggcgg tcctcgccgc aggatcctgg gcatcctgtt
     1561 cggcaacgcc atgaacttct gcgtcctggg gctgacgatc gcactgggca ggagcccggt
     1621 gtggtgggcg gcaggattcg tcgtcggcca gtgcctcggt ggcatggtga atggtctctc
     1681 gcagtccatc tggcaggtca aggttccaca cgaataccag ggcagggtct tcgcatcacg
     1741 cagactgctc gcatcgatca tctcacccgc agctctgctg atctcgggtc ctcttgcaga
     1801 caaggtgttc gagccggcga tggcgccctc tggctggatg gcacgggcat ttggccgatt
     1861 cacaggcacc ggccctggga cgggcatggc gctcatcata ttcatcagtt gcatgctggc
     1921 catgttgacc agccttacgg gcttcgccgt gccaggcatc cgtcacctgg agacgctgat
     1981 cccggacaac gatgcagtcc ctgcgcagga gcctgtacct ggcgactgag agtgcctgcc
     2041 gtctaccgag gtctggtctc cttgccctgc agcatgcgcg agaggaggtc gctcagctgc
     2101 tgcatcgtgt acggcttctc gagtgaggcg tcaaagccgc gcgagatgta gcggtccggc
     2161 aggctggcga ccgaaccgct ggaagcgatt gcgacgatcc cggggtcaag ctgcttgagc
     2221 tctgcgatga tgtcgtagcc gctcttcctg ccagggatgg tcaggtcgag gagtgccgcc
     2281 gtgaacggtt cacccgccgc catgccttca cgcactgcag tgagcccgct gtccccgtcg
     2341 gggacggcaa gcgccttgta gcccaggtgt tcgaggagct gagtggtcgt cgtccggatg
     2401 atggtttcgt catctacgat caggacgcgt ccgctgccct tgacgaggtt cctctgtgct
     2461 cctgtactat ccgctgcagc gactacgtgc ggtcccgaga ggtcggcggg aagcccgaaa
     2521 tcgaaggtcg caccctttct gccggggacg gagaaggcct cgatgatgcc gccgtgcttg
     2581 gcgatgatgg agtagcaggt ggcgagaccc aggccggtcc ctgtctgctt cgtcgtgaag
     2641 tagggctcga agagccgcga cagcgcggca gggtcgatgc cgtgtccttc gtcggtgatc
     2701 gagaccgaga catagggccc gtctggaagc gtgccccatg gctgctccct ggacacctca
     2761 cggttctctg cagtgatcca gatgtgcccg acgccgtcca tggcctgctt ggcgttgagg
     2821 aggaggttca tgaggacctg tgacatctgg ccgcggtcgg cgcggatatc cgcaagctcc
     2881 ggcgatatgt tctcctcgaa ctcgatggca gatccgctgc ttgcaaggcc gacggcattc
     2941 cggatgagcg gccggatgtc gcagcgcgac cggatcggct gccatccctt gtcgaacgtg
     3001 aggagctgac gggccaggtc ccgtgccgag agggcgccgg tctcggcttg cgcgatgtgc
     3061 tgctggactg cgtcgggatg gtcgcggtcg gccctggcga gcgacagccg tcccaggatg
     3121 cccgaaagca ggttgttgaa gtcgtgggcg acgccgcccg cgagcaggcc gatcgactca
     3181 agcttctcgg cgcggctgcg ctgttccagc tctcgttcct ggtcggtgat gtcaagccct
     3241 gtgaggagga ctttggtcat cactcccttc tcgtcggcga gaggcgagac gcggaacacc
     3301 agggttcgcg gatctgattg attcgcgacg acgcggatcg tcatggtgtc ccac
//
LOCUS       DATMZD010000288         7448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_118591,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000288 DATMZD010000000
VERSION     DATMZD010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7448
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118591"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2477
                     /locus_tag="VN478_05965"
     CDS             <1..2477
                     /locus_tag="VN478_05965"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWQ22254.1"
                     /translation="LRLYEGHFRFARVPAQTLLKEDIGTVILADTIVAARLGEYKGVL
                     SLPGVRLVAYDHHEPNSATVVHIDEGHIRKCGATASLVTAALREQLVPVNPDEATLLA
                     LGIYEDTGSLLFPETTAFDVEQAAYLLKAGASLSMVARFVTTYLAPEQQTVLESGLTN
                     MQVRTVAGAQIAFAVVHADRNVGNLSVIVHRLAQIVKAEALFLIYQTPQALYVLGRSD
                     RLLNVAELLAPLGGAGRIHAAASILRDGTSAETAQEQIMSSIAARRSQRRQVVRDIMT
                     PHPATISQDHMAGEALQKMINLGFSVLPVERDGQIVGVVAKKALEKASMSRLQQKPVH
                     YFLNTRVPTVPINATVEELIQAFGSYNTGLLLVMEQERLLGVVSRSDVLRYLNSTDHP
                     QSIVGKPAPRVIANSDLDHLGGDGTSTTLARIGQIVDSRENRAYLVGGSVRDILLGRT
                     PNDFDIVTEKDVSGVATAVARSFDTKVISYGRFMTHKVHIGNREYDFAGARLEYYDFP
                     GSLPTVAPASLMKDLLRRDFTVNALAMSLSQRDFGSVVDATGGLNDLETRTIRMTYPD
                     SFIEDPARILRAVAAQTRLGFALDRGTCEKAKEALELGLLNVRRNKRAQEEFKELLAG
                     GACAMCVKALCILGCGLIGLPLVPPRPARLLLLDALDHEVSVRPQADGAPRWVAPLLV
                     WLGAIPSSEVQSILKDFGLSDRLAGACRSWQSRERSLRRLLAQRACTASAASARLEGI
                     PSPVLSILSARLSISENTGTTNFLKKVRSLMDEPPLISGEDAVALGIPEGRQVGLLLD
                     QVRRLQLDGAITSRDEAMAYLRVSTG"
     gene            2529..2603
                     /locus_tag="VN478_05970"
     tRNA            2529..2603
                     /locus_tag="VN478_05970"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2561..2563,aa:Ile,seq:cat)
     gene            complement(2705..5032)
                     /locus_tag="VN478_05975"
     CDS             complement(2705..5032)
                     /locus_tag="VN478_05975"
                     /inference="COORDINATES: protein motif:HMM:NF019450.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HWQ22255.1"
                     /translation="MRPFVRVVVFLFLCLGLSASGLSGEVAGVLAMGSSGAWYHLPLE
                     EEKVLCVAIDPLTPSILYAGSGGGGVSRSVDGGMTWTAANVGLVDPWVWSLAITPSTP
                     SVVYAGTDGGGVFCSPDSGKTWIAVNEGLTNLHVRSLAINPLAPSILYAGTTGGVFRS
                     TNGGTRWSAADAGLASPQVSSLAVNPLVPSIVYAGTTGGVFRSTNGGDSWTMTSVGLT
                     DRWVWSLAIDPHTPEIVYAGTVGGVFRSANGGTTWSPVNAGLTDLWILSLAVDPLATT
                     TVYAGTGSGGVSCSVDGGMTWTVVNGGLTDPWVWSIAVNPSSSAVVYAGTGGGGLFQY
                     DSMSAYVLHTSVSPSDGGSVSTSPGGAVYARGTVVTLTALAAPGYLFAGWSGDLIGTQ
                     DVAEVTMNADTSVTALFSPTAYALTVDVSGLGSVSRAPDQASYALGTAVQLTADPAPG
                     WEFAGWTGDVTTVMNPVSIVIGGAHALVAQFEPSAASLTSSLHMSVVGEGSVACSPDR
                     EKFEPGETVELRATPATGWRFAEWQGDLSGMGMTVRLPMTSSREVVAVFQRLPEEKQW
                     SVALHCGTHGTITPAGTQNVGDRATVRIRIEPDAGYQVDRLLIDGIPVSLPTTDAQTY
                     DIGPVTADSQVQCTFVAVDPSSQTHLVSFAIGSTWLTIDGRQTALDAAPAIRNGRTFL
                     PVRAIVEAFGGTIAWHAELRVISISLNGRGISLQIGNQQAYVDGIQTPVDASDSNVVP
                     IVISGRTFLPIRFVAESLGLRVEWNPATQTVTVQG"
     gene            5493..6224
                     /locus_tag="VN478_05980"
     CDS             5493..6224
                     /locus_tag="VN478_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22256.1"
                     /translation="MLIDKLWELQELQLDYRRKERLLEELDLSSLLTHKIDETEQQLA
                     GLREQRETFRADETKLTEKHDDLSAKVHEAGAKLGKEVVSEDKVQKLEEKMKKLTLEK
                     EKVEDAQAKNFHDRQDVEDQIRGSELMVEKLRKEFESIPQSDATSRAAVKKSLPTLRK
                     EIENRKAKLPLKAKLIFENLSEKFSGAPIARATSSTCDYCHVRLSQKTLFSLKNVDGN
                     GGDAEDLIRCEICGKILYAGTKDRE"
     gene            6214..6533
                     /gene="rnpB"
                     /locus_tag="VN478_05985"
     ncRNA           6214..6533
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VN478_05985"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(6687..>7448)
                     /gene="tpiA"
                     /locus_tag="VN478_05990"
     CDS             complement(6687..>7448)
                     /gene="tpiA"
                     /locus_tag="VN478_05990"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HWQ22257.1"
                     /translation="MKTVAGNWKMFKTIEEAREYFAQLAADVHPPGDKVHVLIFPNYV
                     ALGALAGTDEVPSWVLLGAQDVAAEAEGAFTGEVSPAMIRSAGATHVLIGHSERRHIL
                     GESADLLHRKLANALSASLIPVLCVGETLEERTAGKMDDVVFGQLDTALCDVELSSGA
                     QLIVAYEPVWAIGTGVNATNEQIEQAHLLIREHLIQLLGTAIGSSVAILYGGSVKPAN
                     FASIVGLPSVDGGLIGGASLKPSAFGELIEIAEQV"
BASE COUNT         1592 a         2231 c         2205 g         1420 t
ORIGIN      
        1 tcctccggct ctacgagggg cactttcgtt ttgcgcgcgt gcctgcgcag acactgctca
       61 aggaagacat tgggactgtc atcctggctg acacgattgt tgcagcccgt cttggcgaat
      121 acaaaggagt cctgtcgctc cccggggtaa ggctggttgc ctatgaccat cacgagccaa
      181 actcggcaac cgtggtgcat atcgacgagg ggcacatccg aaagtgcggt gctactgcgt
      241 cacttgtgac ggcggctctc cgtgaacagc tcgttccggt caaccctgac gaggccacac
      301 ttcttgcatt gggcatctac gaagacacgg gctcgctcct gtttccagag accacagcgt
      361 tcgacgtcga acaggcagca tacctgctca aagccggcgc aagtctgtca atggtcgctc
      421 gctttgtcac gacctacctg gccccggaac agcagacggt tctcgagagc ggcctcacga
      481 acatgcaggt tagaacggtt gcgggggcgc agatagcgtt cgcagtggtt catgcagatc
      541 ggaacgtagg caatctttcg gtaattgttc accggctggc tcagatcgtc aaagcagaag
      601 ccttgtttct gatttaccag actccgcagg cgctctacgt cctcgggcgc agtgaccgcc
      661 tgctcaatgt tgcggagttg ctcgccccgc tgggtggggc aggtcgaatc catgcagcgg
      721 catccatcct gagagacggc acgtctgctg agaccgctca agagcagatc atgtcctcga
      781 ttgcagcgag gcgcagccag cggcgtcagg tcgttcgcga tatcatgaca ccgcatcccg
      841 ccacgatttc tcaggaccac atggccggag aggctctgca gaagatgatc aacctcggat
      901 tctccgttct gccggtcgag cgcgacggcc agatcgtcgg cgtcgtcgcg aagaaggcgc
      961 tggagaaggc ctccatgtcc cggctacagc agaagcctgt gcactacttc ctcaatacgc
     1021 gcgtcccgac ggttccgatc aatgcaaccg tcgaggaact gatacaggca ttcggctcct
     1081 acaacactgg tctgctgctc gtcatggaac aagagcgtct gctcggcgtg gtatcgaggt
     1141 ccgacgtcct gcgctatctc aacagcacag accacccgca gagtattgtg ggcaagcccg
     1201 ctccccgcgt gatcgcgaat tctgatctgg atcatctggg tggagacggc acgtcgacga
     1261 cactggccag gatcggtcag atcgtggaca gcagggagaa tagggcatac ctggtcggcg
     1321 gatcggtccg cgacatcctc cttggcagga ctcccaacga cttcgacatc gtcaccgaga
     1381 aggacgtgtc gggggtagcg acagcggtcg caaggtcttt cgacaccaag gtgatctcgt
     1441 atggacgatt catgacacac aaagtccaca tcgggaatcg agaatacgac ttcgcaggcg
     1501 cccgtctcga gtactacgac tttcctggct cgctcccaac ggtcgcccct gcctcgttga
     1561 tgaaggatct tctgagaagg gacttcaccg tgaatgctct cgccatgtcc ctttctcagc
     1621 gtgactttgg ctctgtcgtc gacgccacgg gcggactgaa tgatctcgag actcggacga
     1681 tccgcatgac ctatcccgat agcttcattg aagacccagc gcggattctc cgagctgtag
     1741 cggctcagac ccggctgggc ttcgcacttg acagaggcac gtgcgagaag gcgaaggagg
     1801 ctctcgaact cggactcctg aatgtgcgcc gcaacaagcg ggcacaggag gagttcaagg
     1861 aactgctcgc aggtggcgct tgcgccatgt gtgtcaaggc gctctgcata ctcggatgtg
     1921 ggcttatcgg ccttcctctt gttcccccgc ggcccgccag actgctactt ctcgacgcgc
     1981 ttgaccacga ggtctctgtc cgcccgcaag ccgatggtgc acctcggtgg gttgctcctt
     2041 tgctcgtatg gcttggcgct atcccttcca gcgaggtgca gagcatcctg aaggacttcg
     2101 ggttgagtga ccgcttggca ggcgcatgtc ggtcctggca gagcagagag cgctcattgc
     2161 gccgcctgct cgcacagcgg gcatgtactg cgagcgcggc atccgccaga ctcgaaggca
     2221 tcccgtcgcc cgttctatcc atcctgagcg cacgcctgtc gatctcggag aacaccggca
     2281 ccaccaactt cctcaagaag gtgcgttcac tgatggacga accgccgctc atctccggtg
     2341 aggacgccgt ggcccttggc atccctgagg gacggcaggt cgggttactt ctcgaccagg
     2401 tcaggcgcct ccagctggat ggcgccatca catcccggga cgaagccatg gcctatctgc
     2461 gcgtctcgac aggttgaccc agaggactcc tccggtataa tactggagcg gcgcaagact
     2521 tgtcatgggg gcccatagct caacggtaga gcaggtggct cataacctct tggttcttgg
     2581 ttcgaatcca ggtgggccca ccacttcgct tgtgcctccg gcgacagccg actccgcgta
     2641 gtgccctggg gagtgccccg tctggtacgt cggaatagcg tggtgtgccc gacattgcca
     2701 tgcgtcagcc ttgtaccgtc accgtctgtg tagcgggatt ccactccacc cgcagtccga
     2761 gactctcagc gacgaaccga atgggcagga acgtcctccc ggatatcacg atcggaacca
     2821 cgttcgaatc ggatgcgtca acaggcgtct gaattccatc gacgtatgct tgctggttcc
     2881 caatctggag actgatcccc cggccgttga gcgagatgga gatgacacgc aattcggcat
     2941 gccatgcgat ggttccgccg aacgcttcga caatagcacg gaccggcagg aaggtcctgc
     3001 cgttgcgaat tgctggggca gcatccagtg cggtctgtct cccatcgata gtcaaccatg
     3061 tcgacccgat cgcgaagctg acgagatgag tctgcgagga agggtcgacg gctacgaagg
     3121 tgcactgcac ctgactgtcc gccgtgacgg gccctatgtc gtacgtctga gcgtcggtcg
     3181 tcggcaacga gacgggaatc ccgtcaatga ggagtcggtc aacctgatag cccgcatcgg
     3241 gttcgatcct gatccgtaca gttgccctgt cgcccacgtt ctgcgttccg gctggagtga
     3301 ttgtgccgtg tgtcccgcaa tgcaacgcca ctgaccactg cttctcttca ggaagcctct
     3361 ggaataccgc cacgacctcc ctggatgaag tcatcggcag ccgtaccgtc atgcccatgc
     3421 cgctcaggtc tccctgccac tccgcgaatc gccacccggt cgccggagtc gcccgaagtt
     3481 ccactgtctc cccgggttcg aacttctcac gatcgggtga gcacgccaca gaaccctctc
     3541 cgacgacaga catgtgaagt gatgacgtca acgaagcagc cgaaggctcg aactgagcca
     3601 caagggcatg cgctccacca atgacaatcg acacggggtt catgacagtc gttacgtctc
     3661 ccgtccatcc ggcgaactcc catcctggag caggatccgc ggtcagttgc accgcagtgc
     3721 cgagcgcata tgaagcctgg tccggcgccc tggacacaga gccaagaccg gaaacgtcga
     3781 cggtgagagc gtaggcggtg gggctgaaga gcgcagtaac ggacgtgtcg gcgttcattg
     3841 taacctcggc cacatcctgg gtaccgatca ggtcccccga ccaaccagcg aacagatagc
     3901 cgggagcagc cagagcagta agggtgacga ctgtgccacg agcatagacg gcaccgcccg
     3961 gagatgtgct gactgagccg ccgtctgaag gcgagacgga cgtgtgcagt acatacgctg
     4021 acatggagtc atattggaac aatccgccac cgccggtgcc ggcgtagacg acggcagagg
     4081 atgaaggatt gacggcgatg gaccacaccc atgggtcagt caacccccca ttgacaacgg
     4141 tccaggtcat ccccccgtcc acggagcatg agacgccgcc acttccggtc ccagcgtaga
     4201 ctgtcgtagt cgcgagcggg tcgacggcga gagagaggat ccagagatca gtgagtccgg
     4261 cgttcactgg actccaggtg gttccgccgt tggcagaacg aaagacgccg cctacagtgc
     4321 ccgcatacac gatctcaggc gtgtgagggt cgatcgccag ggaccacacc catcgatcag
     4381 tgagtccgac gctggtcatt gtccagctgt cgccgccgtt ggtggagcga aagaccccgc
     4441 cggtcgtgcc tgcatatact atgcttggca caaggggatt caccgcaagg gagctgactt
     4501 ggggactggc gagccccgca tctgctgcgc tccatctggt gccgccattg gtggatcgga
     4561 acacgccgcc tgtggtgcct gcatagagga tgctcggcgc aagggggttg atggcgagag
     4621 atctgacatg caggttggtc agcccctcgt tcaccgcgat ccacgtcttc ccgctatcgg
     4681 gagagcagaa cacgccaccg ccgtcggtcc cagcatacac gacgcttgga gtggaggggg
     4741 tgatcgccag ggaccagacc cacggatcaa ccaaccccac gtttgccgcc gtccacgtca
     4801 tcccaccgtc gaccgagcga gagactccgc ctcctcccga gcccgcgtac agtatcgacg
     4861 gggtcaatgg gtcgatggca acgcacagaa ccttctcttc ctccagcggc agatgatacc
     4921 aagctcctga agaacccatg gcgagcactc cggcgacctc gcctgacagg ccagaggcac
     4981 taaggccgag gcacagaaac aggaatacga cgacgcggac gaatggtctc attatcgcgg
     5041 actcctctgg cgtccatttc ctgctgacaa cgctgtcatg gctaaactgg agtatagtcg
     5101 cgggtccggt cacggtcaag cgtgcggttc ctcgaacgca tgaggccaag gcgtactcag
     5161 accaccatac agccccttca tgccacaagc tgagcatttt gccgacgcgg ccattcttcg
     5221 acacacgagg attgcatatt ggacctagtc ggctagaata ctacaggact agatacacag
     5281 gcgcgtgcgc ctggcagcag gcagactgtc cccgggagtg ctcatgcacg ctgacaggca
     5341 cttgtggtat ctgggtattg ctggttccag tcaacagtat cgatagtctg cccaaatgac
     5401 tgaccgatag tcagcggtcc tgtcgccgat ttggctgcga gggagatcgc cggtcttcac
     5461 caagatgtca caattctcga tgaggtgtag tgatgctgat agacaagctt tgggaacttc
     5521 aggaacttca actggactat agacggaaag aacggcttct cgaggagttg gatctctctt
     5581 cgttgctgac tcacaagatt gatgagactg agcagcagct cgctggactg cgtgagcaga
     5641 gagagacgtt cagagccgat gagaccaagc tcaccgagaa acacgatgac ctgtctgcga
     5701 aggttcatga agccggagcc aagctcggga aagaagtagt gtcagaggac aaggtccaga
     5761 agctcgagga gaagatgaag aagctgactc tcgagaagga gaaggtcgag gacgcccagg
     5821 cgaagaattt ccacgaccga caggacgtcg aggatcagat caggggatcg gagttgatgg
     5881 tcgaaaagct ccgcaaggag ttcgagagca ttccacaatc ggatgcaact tccagggcgg
     5941 ctgtcaagaa gtctcttccg actctgcgca aggagatcga gaatcgcaag gcaaagctcc
     6001 cgctcaaagc caagttgatc tttgagaacc tttcggagaa gttcagcggg gcaccgatcg
     6061 ccagggcgac cagttcgaca tgtgactatt gccacgtgag actttctcag aagacactgt
     6121 tcagcctcaa gaatgtggac ggcaacggcg gtgatgctga agatctgatc cgttgcgaga
     6181 tatgtggcaa gatcctgtac gccggcacca aggaccgcga atgagtcagt cgcaggcagg
     6241 agatcttccc gcctgaggaa agtccgggct ccaagaagcg agttgctcgg gaaacccgag
     6301 tgggggtaac cccaaggaaa gtgcaacaga aaatataccg ccccgcaagg ggtaagggtg
     6361 aaatggcggc gcaagagacc accggcagac cagtgatggg ctgcgcattg caaaccccaa
     6421 ctggagcaag acaaaatagg aaggcacacg gggctgcttc tccccgcctt cgggtatgtc
     6481 gcttagataa atgactgacc acgacagaac ctggcttatc attcgcggta tgtgaagaga
     6541 ccccgaccgt acttcggaag gggtctctgc gtttgcgaga acatgaagag accccgaccg
     6601 tacttcggaa ggggtctctt gcgttaccgc tgttggtgct tgcagactct cagaacgagt
     6661 gtggcaaacg agacattgcg ccgaagctac acctgctcgg ctatctcgat cagttcgccg
     6721 aacgccgacg gcttgagact ggctcctccg atcagcccac cgtccaccga cggaagacct
     6781 acgatggatg cgaaattggc gggcttgacg ctcccgccat acaggattgc cacggagctg
     6841 ccaattgcag tgccgagcag ctgtatgaga tgctcgcgaa tgagcagatg cgcctgctcg
     6901 atctgttcgt tcgtggcatt gacccccgtt ccaatggccc acaccggttc gtaagccacg
     6961 atgagctgtg ccccgctcga cagctcgacg tcacaaaggg ccgtatccag ctgaccgaat
     7021 acgacgtcgt ccatcttgcc cgccgtccgc tcctcaagcg tctccccgac gcagagaacc
     7081 ggaatcagtg acgcactcag agcattggcc agcttgcggt gaagaaggtc tgctgactcg
     7141 ccaaggatgt gcctgcgctc cgaatgcccg atgagaacgt gggttgcccc tgcggaacgg
     7201 atcatggccg gcgagacttc tccagtgaac gctccctctg cttcagctgc gacatcttga
     7261 gcacccagaa ggacccacga gggaacctcg tcggtgcccg ccagcgcccc gagcgcgacg
     7321 tagttcggaa agatgagcac gtgcaccttg tctcccggag gatgcacgtc ggctgccagc
     7381 tgtgcgaagt actctcgggc ttcctcgata gtcttgaaca tcttccagtt tccggcgacg
     7441 gtcttcat
//
LOCUS       DATMZD010000289         7622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_139701,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000289 DATMZD010000000
VERSION     DATMZD010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7622
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139701"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            247..705
                     /locus_tag="VN478_05995"
     CDS             247..705
                     /locus_tag="VN478_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HWQ22258.1"
                     /translation="MAYVFEIVESQPQPALWIRKLTSVDRLPQELGKAYGAIITYLTE
                     KGVQAQGPAFTAYFNMDMESLEVEMGFPVATEMVGSGDIVAGYIPGGRKATCMFKGPY
                     RDMGPTYEALTKWIGDNGYVPVGTVYESYYNSPTEVPESELLTKIEFLLK"
     regulatory      1104..1221
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            1211..3022
                     /locus_tag="VN478_06000"
     CDS             1211..3022
                     /locus_tag="VN478_06000"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HWQ22259.1"
                     /translation="MKRGLSRLLVRRVLATALILAFVSIASALPARAASLSDIVGRPD
                     QAVIERVIAKGFMKGYPDGTFRPNAPVSRAEFIVSLARSAGLDPVLSPAKTLKDLPIS
                     NWAAPLVYAALAKGWVSGYPDGTFRPTASITREESMAVVAQRMGFEIRGMATHTVLDK
                     VAGSEDLSQWARTAAAACVQEGMVSSVAKPTTAITRAQTAAYFPAIMDYKAPETQSMI
                     LATTTSTFDSGLLTFLVPIYEKSNHVKVKIISVGTGQAIATGRKGDADVLLVHARAQE
                     DLFMADGYGLFRKDVMYNDFIIVGPASDPAKIKGMTDAAAAFARLAASGSTFISRGDK
                     SGTNTKELDIWKSAGIAPAGKSWYLNAGQGMGEVLMMTDQKQAYTLTDRATYLAYQNG
                     GKIDLPILVQGSQGLLNPYGLMPVNPAKYPTVKVDLAIRFTDFMTGLPGQELIKSFGV
                     EQYGQTLFYPDSTEWKATRSLDIVLPTGKEVTYHLGDVRKFPVTTVKVYPQNNQSLTP
                     VPYGGAALIDILKTISPDLASARRLTVIATATDGSHVTLDYDDIYNTRTGTKITVVYL
                     KNGTTLSTDEGFMALMTTRGAFLVSSIKYVYRLEIHK"
     gene            3049..3768
                     /locus_tag="VN478_06005"
     CDS             3049..3768
                     /locus_tag="VN478_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012901546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWQ22260.1"
                     /translation="MGDIGRGFVQAVRLILSLDPGTYEALGTSLRVTMTALFWSALAG
                     FPLGILLAFSHFRGKQGVMNVVYTLMGFPPVVAGLLVYLLLSRSGPLGALGLLYTPTA
                     MVTAQIALALPVIVGLSVLGLRGADKGVFDLALTLGATRAQASIALLREARLAILGAV
                     ITAFGNAISEVGAAMLVGGNIQGATRTLTTALVLETRQGSFDRAIALGIILLLVSFLV
                     NVVLSRLGEGTSEAGNVRHID"
     gene            3749..4534
                     /locus_tag="VN478_06010"
     CDS             3749..4534
                     /locus_tag="VN478_06010"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWQ22261.1"
                     /translation="MSATSTDLITARGVRKRFGPREVLRDIDLTVVAGNITVILGTNG
                     AGKTNLLRILGLLEPADEGTVAYDGIVPGVSTITYGASAKSSAQDGRLLPLRRQMLLA
                     FQHPAIFSGSVLSNVTYGLQLRGMSRPDAEERSMAALGEAGIADRARQHASSLSGGEA
                     ARLSVARALALQPRLLFLDEPSASLDPYGTKLLEDLLLHMRDDLGMTAVVVTQDIFEA
                     RRVADQVAFLHEGRIIEQAPRETFFTNPHDDRTRRFAAGELLV"
     gene            4584..5813
                     /gene="glp"
                     /locus_tag="VN478_06015"
     CDS             4584..5813
                     /gene="glp"
                     /locus_tag="VN478_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005031626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HWQ22262.1"
                     /translation="MLSIEEALKAILDHATPLTPVAMPLDEAAGLVLAETVTARGDVP
                     PFANSAMDGFAVQAADIQQASEATPVRLHILEDVPAGSVATQPVRPGTAIRIMTGAPL
                     PGGADTVVHVEVTRTEGMDVLVLLALKPGINVRGAGEDMKDGATVLAAGTVLRPGEVG
                     VCAAAGHATVTVFPRARVAVLTTGSELVDAAQVPGPGQIRDANAHSLRAQVTAMGAVP
                     VVFDRVPDTREAVRSALDQALLQADVILTNGGVSVGDYDFVKDVLQEMGAELVFWRVK
                     QKPGKPMAFWTLGSKRIVGLPGNPVSCMVCAEEYVRPLVRRMMGQALLHRPVRTAVLD
                     ERYAKGSDAGRAHFVRVRLQERGGVLHAISTGPQGSGILTSMARATGIAVLPEDTPVV
                     EAGQSVRVQLTDLPEDH"
     gene            5813..6778
                     /gene="moaA"
                     /locus_tag="VN478_06020"
     CDS             5813..6778
                     /gene="moaA"
                     /locus_tag="VN478_06020"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015761001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HWQ22263.1"
                     /translation="MIDRFGRTIDYIRISVTDRCNLRCRYCMPEEGVPMLEHEDILRF
                     EEIVAFMRVAVSEGIVKVRLTGGEPLRRPGVVSLVRMLLDVPGLQDLSLTTNGVLLPQ
                     FADELKAAGLKRVNIGLPSLDPTVYADLTRLGSLDKALAGLKAAVDTGFSPIKLNVVV
                     LRGINDDLAPYLELTRHLPVDVRFIEYMPIGPLGEDHYLVPAAELRRKLETLAPLETA
                     DKPSGNGPAQEYRRIPGAPGTVAVIAPVTEHFCPTCNRLRLVADGHLRLCLFGADEID
                     IKPAMRPVVNEEALRALLVQAMQQKPECMPETARDYGRVMGSIGG"
     gene            6780..7262
                     /gene="moaC"
                     /locus_tag="VN478_06025"
     CDS             6780..7262
                     /gene="moaC"
                     /locus_tag="VN478_06025"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HWQ22264.1"
                     /translation="MRGNELTHLDDKGNARMVDVGGKLETAREAVARCRVLLEPATFE
                     LIKAGNLKKGDVLTVAQVAGIQAAKRTWELIPLCHQIAMTFVGVSLTLNEDDHAVDIE
                     ATARTKAETGVEMEALVAASAAALTVYDMCKAVERGIRITDLRLVRKSGGQSGTWEGA
                     "
     gene            7259..>7622
                     /locus_tag="VN478_06030"
     CDS             7259..>7622
                     /locus_tag="VN478_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017212878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HWQ22265.1"
                     /translation="MSDPVVVSVNISEHKGTRKHPLQGPVTITVLGMQGDAHAGDWHR
                     QLSLLAQESIDKMQGHGLVLKPGDFAENITTQGVDLLTLPVGTRLQIGGSVTMEITQI
                     GKECHLGCEIRKLVGDCVM"
BASE COUNT         1437 a         2519 c         2298 g         1368 t
ORIGIN      
        1 cgtttccccc ctgcgccgcc gagaggccac gtgctcaaac cgcgcggagg cagatgtgtt
       61 gggtataccg ccaaactgtc ggtcggtcaa gcagtgcatt gtcacaaccg cgtcagggtc
      121 cggaaactgg catcagcggc actattacca ggaacgccgg gctccgacag caagtccctt
      181 gaatagttga cagagagtag tagtctcatt tcagcagtat tcagctggag gatggaggtt
      241 cttgacatgg cgtacgtatt cgagatcgtc gagtcacagc cgcaacccgc tctttggatc
      301 agaaagctga cttccgtgga ccgtctgccc caggaactcg gaaaggccta cggcgccatc
      361 atcacatatc tgaccgaaaa gggagtacaa gctcagggac cggcctttac agcctacttc
      421 aacatggata tggagagcct cgaggtggag atggggttcc cggtcgccac ggagatggtc
      481 ggcagcggcg acatcgtcgc tggttacatt cccggcggca ggaaagcgac ctgcatgttc
      541 aagggaccgt acagggacat gggtcccacc tacgaagccc tgaccaagtg gatcggtgac
      601 aatggatatg taccagtcgg caccgtctat gaatcctact acaattcacc cacagaggtg
      661 cccgaaagcg agctgctgac caagatcgag ttcctgctca agtagcacca tacagcaggc
      721 ttcatctgtc gttcgcccgg accctcttat ccagggtgaa ggcgcttccc cccgagtatt
      781 tctgtgtaat acgaggggcg ttcttggcaa aggcagggat atgcgcctcc agaccaggac
      841 tggattcccg catgcgcggg aatgacacat caactgcctt ccatccgtcg ttccccctga
      901 gtatttcagt tcaatgcgaa gggcgctttc ccccgagtat ttctgtgtag tacgaggggc
      961 gttcctggcg aaggtgggaa cctattccct atgccacgac tggatttccg catgcgcggg
     1021 aatgacgtgc cgagaaggac cagcctccga ttcttgccgt gcatgcttcg ccgcctatac
     1081 tgttgttctc tgcggctctg ctgctgagga cgattcttcg agctgtccga ccttaggcgc
     1141 aagccatggc gtacagccgg cagggtatgg cgggaaacgg ccatgcctcc cattgtggaa
     1201 aggagaaaca atgaaacgag gtctgtcacg tctgctggtc cgtcgcgtcc ttgcgacggc
     1261 gctcatcctt gcgttcgtgt ccattgcgtc cgccctgccg gcgcgcgccg catcactgtc
     1321 cgacatcgtt ggacgccccg accaggccgt catcgagcgg gtcatcgcca aagggttcat
     1381 gaagggatac ccggacggga cgttccgtcc caacgctccg gtcagccgtg ccgagttcat
     1441 cgtgtcgctt gcacgctcag ctggactcga ccccgtgctg tcgcccgcga agacgctcaa
     1501 agacctgccc atcagcaact gggctgcccc tcttgtctac gctgccctcg ccaaaggctg
     1561 ggtctccggc tacccggacg ggacgttccg cccgacagcc agcatcaccc gcgaggagtc
     1621 gatggctgtc gtcgcacagc gcatgggctt cgagatccgc ggcatggcga ctcataccgt
     1681 cctggacaag gtcgcaggct ccgaggacct ttcgcagtgg gcgcggactg ctgctgcagc
     1741 gtgcgtgcag gaaggcatgg tctccagtgt cgccaagccg acgactgcca tcacacgcgc
     1801 tcagactgcc gcgtacttcc ccgccatcat ggactacaag gcgccggaga cacagtcgat
     1861 gatcctcgct acgacgacct ccaccttcga ctcaggactc ctgaccttcc ttgtccccat
     1921 ctatgagaag tccaaccatg tcaaggtcaa gatcatctcg gtcggcactg gtcaggcgat
     1981 cgcgaccgga cgcaagggtg acgcagatgt cctgctcgtt cacgcgcgcg cgcaggaaga
     2041 tctgttcatg gctgacggct atggcctctt ccgcaaggac gtcatgtaca acgacttcat
     2101 catcgtgggg ccagccagtg accctgcgaa gatcaagggc atgaccgacg cggcggcggc
     2161 gttcgcgaga ctcgccgcat caggctccac gtttatcagc cgaggcgaca aatcgggcac
     2221 caacacgaag gagctggaca tctggaagtc cgccggcatc gcgccggccg gcaagtcctg
     2281 gtatctcaac gccggccagg gcatgggcga ggtcctcatg atgaccgatc agaagcaggc
     2341 gtacacgctg accgaccgcg ccacatacct cgcctaccag aacggtggca agatcgacct
     2401 gcccatcctc gtccagggct cccagggact gctcaaccct tacggcctca tgcccgtgaa
     2461 tcccgccaaa tacccgacgg tcaaggtcga cctcgcgatc cgcttcaccg acttcatgac
     2521 cggtcttccc ggtcaggaac tcatcaagag cttcggcgtc gagcaatatg ggcagaccct
     2581 gttctatccg gattcaacgg agtggaaagc cacccgcagc ctcgacatcg tgctccccac
     2641 cggcaaggaa gtcacgtacc atctgggcga cgtgcgcaag ttcccggtga cgaccgtcaa
     2701 ggtctacccg cagaataacc agtcgctgac gccggttccc tacggtggag cagccctcat
     2761 cgatatcctg aagaccatct cgcccgatct cgcatctgcc aggcgtctga ccgtcattgc
     2821 cacagctaca gacggcagtc acgtgacgct ggattacgac gacatctaca atacacgtac
     2881 gggaacgaag atcaccgtcg tgtacctcaa gaacgggacg acactctcga cggatgaagg
     2941 tttcatggca ctcatgacga ccaggggcgc attcctggtc agcagcatca agtacgtgta
     3001 ccggctcgag atccacaagt agcatgaaca caccgcggag ggacacgaat gggtgacatc
     3061 ggccggggct tcgtccaggc cgtccggctc atcctctccc tggaccccgg aacgtatgag
     3121 gccctgggga catccctccg cgttaccatg accgccctgt tctggtcggc gctggccggg
     3181 ttccctctgg gcatcctgct cgcattctcg cacttccgtg gcaagcaggg cgtcatgaac
     3241 gtggtctaca ccctgatggg gttccctccc gtcgtggcag gcctcctcgt gtatctcctg
     3301 ctgtcccgaa gcggccctct gggagcgctt ggactgctct acacgccgac ggcgatggtg
     3361 accgcccaga tcgcgctggc gctcccggtc atcgtgggac tctcggtcct tggactgcgc
     3421 ggcgccgaca agggcgtctt tgacctcgcg ctcacacttg gtgcaacacg cgcacaggcc
     3481 tccatcgcct tgctccgcga ggcgcgcctg gccattttgg gggccgtcat cacggcattt
     3541 ggaaacgcta tcagcgaagt aggcgcggca atgctggtgg gcggcaacat ccagggagcg
     3601 acccgtacgc tcacgacggc gcttgtcctc gagacccgtc agggcagctt cgaccgcgcc
     3661 atcgctctgg gtatcatcct cctcctcgtc tcgtttctcg tcaacgtggt cctttcgcgg
     3721 ttgggtgagg gcacgtcgga ggccggcaat gtccgccaca tcgactgatc tcatcaccgc
     3781 gagaggcgtg cgcaagcgct tcggtccccg ggaagtcctc cgggacatcg acctcaccgt
     3841 cgtcgcaggt aacatcactg tcatcctcgg cacgaacggc gccggcaaga cgaacctcct
     3901 gcggatcctc ggactgctgg agcccgccga cgaaggcacc gtagcgtacg acggcattgt
     3961 ccccggcgtg tcgaccatca cgtacggcgc ctccgcgaaa agttccgccc aggatggacg
     4021 cctcctcccc ctgcggcggc agatgctgct cgcctttcag caccctgcta tcttcagcgg
     4081 ctcggtgctc agcaacgtga cgtacgggct tcaactgcgc ggcatgagcc gtcccgatgc
     4141 agaggagcgg tcgatggctg cgctgggcga ggcaggcatc gccgatcgcg cccgccagca
     4201 tgcctcatcg ctttccggcg gcgaagcggc ccgcctgtcg gtggcgcgcg cccttgccct
     4261 gcagccacgc ctgctcttcc tcgacgaacc atccgccagt ctggacccct acggaaccaa
     4321 gctgctcgaa gacctgttgc tgcatatgag ggacgacctg ggcatgacag ccgtcgtcgt
     4381 gacccaggac atctttgaag cccgtcgcgt cgccgaccag gtagcgttcc tgcatgaggg
     4441 gcgtatcata gagcaggcgc cgcgcgagac cttctttacg aaccctcatg acgaccggac
     4501 ccgccgcttc gcagcaggtg agctgcttgt gtaaccatgg ggacagaccc cattatggta
     4561 caagatctgc cactggagga gtaatgctta gcatcgagga agcactgaag gcgatactgg
     4621 accacgcgac gccgctgaca cctgttgcca tgccactgga tgaggccgca gggctggtac
     4681 ttgccgagac agtgacagcg cgcggcgacg ttcccccctt cgccaactcc gccatggacg
     4741 ggtttgctgt ccaggccgca gatatccagc aggcttccga ggccacgccc gttcgcctgc
     4801 acattctcga agacgtcccc gcaggcagcg tcgcgacgca gccggtccgc cctggcacgg
     4861 ccatccgcat catgaccggc gcacccctcc ctggcggtgc tgacacggtt gtccacgtgg
     4921 aggtgacccg cacagaaggc atggacgtgc tggtcctcct ggcgctcaag cctggcatca
     4981 acgtacgagg ggcgggcgag gacatgaagg acggtgccac cgtccttgca gccggcactg
     5041 tcctgcgacc cggcgaggtc ggcgtctgcg cagcagccgg tcatgccact gtcaccgtct
     5101 tcccccgtgc gcgggtggca gtcctgacca ccggcagcga gctggtcgac gccgcacagg
     5161 tgcccgggcc cggacagatc cgcgacgcca acgcacattc gctgcgcgcg caggtcaccg
     5221 cgatgggagc cgtaccggtc gtcttcgacc gtgtgcccga cacgcgtgag gcagtacggt
     5281 cggcactcga tcaggcactc ctccaggctg acgtcatact caccaacggc ggcgtatcgg
     5341 tcggcgatta tgacttcgtg aaggacgtcc tgcaggagat gggcgcagag ctggtcttct
     5401 ggcgtgtcaa gcagaagccc ggcaaaccga tggcattctg gaccctgggc tcaaagcgca
     5461 tcgtcggact gcccggcaat cccgtgtcct gcatggtgtg tgccgaggag tacgtgcgcc
     5521 ccctcgtgcg ccgcatgatg ggccaggccc tgctgcaccg cccggtccgc actgccgtcc
     5581 tcgacgaacg gtacgccaag ggctccgacg ctggacgtgc ccacttcgtg cgggtccgcc
     5641 tgcaggaacg gggcggtgtc ctccacgcca tatccaccgg cccccagggg tccggcatcc
     5701 tcacatccat ggcacgtgcg accggcatcg ccgtcctccc cgaggacacg cccgtcgtgg
     5761 aagcaggtca aagcgttcgc gttcagctca ccgacctgcc ggaggaccac tgatgatcga
     5821 ccgttttgga cgtaccatcg actacatccg catctccgtc actgaccggt gcaacttgcg
     5881 gtgccgctac tgcatgcccg aagagggtgt gcccatgctg gagcatgagg atatcctccg
     5941 gttcgaggag atcgtcgcgt tcatgcgcgt cgccgtctcc gaaggcatcg tcaaggtgcg
     6001 actcactggt ggcgaaccgc tgcgccgccc gggggtcgtg agcctggtgc gcatgctgct
     6061 ggatgtccct gggctgcagg acctctcgct gaccacgaac ggggtcctgc tgccacagtt
     6121 cgccgacgaa ctgaaagccg ccgggctgaa gagagtcaac atcggcctgc cttcgctaga
     6181 ccccactgtc tacgctgacc tcacgcgtct cggctcgctg gacaaagcac tggctggcct
     6241 caaggcggca gttgacacag ggttcagccc catcaagctc aacgtcgtcg tcctgcgggg
     6301 catcaatgac gacctggcgc cgtacctcga gcttaccagg caccttcctg tcgacgtccg
     6361 cttcatcgag tacatgccta tcggcccgct gggtgaggac cactacctgg tacccgcggc
     6421 cgaactgcgg cgcaagctgg agacgctggc acctctggag actgccgaca agccaagcgg
     6481 caacggtcca gcacaggagt atcgccgcat cccgggagca cctgggactg ttgctgtcat
     6541 tgcgcccgtc accgagcact tctgccccac ctgcaaccgc ctgcgcctgg tcgctgacgg
     6601 ccacctgcgg ctgtgtctgt tcggagccga cgagatcgac atcaagcctg ccatgcggcc
     6661 cgtcgtcaac gaggaagcgc tgcgcgcact gctcgtgcag gcgatgcagc agaagcctga
     6721 gtgcatgccc gagacggccc gcgactacgg ccgcgtcatg ggcagcatag gaggttagca
     6781 tgaggggtaa cgaactgaca catctggacg acaagggaaa cgcccgtatg gtcgacgtcg
     6841 gaggcaagct ggagacagcc cgcgaagccg tcgcccggtg ccgtgtcctg ctggagcccg
     6901 ccacgttcga gctgatcaag gcgggcaacc tcaagaaggg cgacgtcctg acggttgcac
     6961 aggtggccgg catccaggcc gccaaacgca catgggagtt gatccctctg tgccaccaga
     7021 tcgcgatgac attcgttggg gtgagcctga cactgaacga ggatgaccat gcggtggaca
     7081 tcgaagcgac cgcgcgcacc aaggccgaga ccggcgtcga gatggaagcc ctggtcgccg
     7141 cctccgccgc cgccctcacc gtctacgaca tgtgcaaggc tgtggagcgg ggtatccgca
     7201 tcaccgactt gcgcctcgtc cgcaagagcg gtggacaatc cgggacatgg gagggtgcat
     7261 gagcgacccc gtcgtggtct cggtcaacat ctccgaacac aagggcacgc gcaagcaccc
     7321 cctgcagggt cctgtcacga tcaccgtgct gggcatgcag ggcgatgccc atgcaggcga
     7381 ctggcaccgt caactcagcc tgctggcaca ggagagcatc gacaagatgc agggccacgg
     7441 cctggtcctg aagcccggcg atttcgccga gaatatcacc acgcaaggcg tggacctgct
     7501 gactctcccg gtcgggacgc gtcttcagat cggcggctcc gtcacgatgg agatcaccca
     7561 gatcggcaag gaatgccacc tgggctgtga gatccgcaag ctggtcggcg attgtgtcat
     7621 gc
//
LOCUS       DATMZD010000290         5103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_39671,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000290 DATMZD010000000
VERSION     DATMZD010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5103
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39671"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            57..611
                     /locus_tag="VN478_06035"
     CDS             57..611
                     /locus_tag="VN478_06035"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015181291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HWQ22266.1"
                     /translation="MHINTNGDPGRITLELGDITRFRGDVIVNAANESLLGGGGVDGA
                     IHAAAGPALLAECRTLHGCATGRAEITGGYHLPARYVIHTVGPVWHGGARGEEELLES
                     AYRSCLELAQRHDLRTIAFPAISTGAYGYPLEKATRVALRTILTWLDEHVLPEHVIFV
                     CYDQRTLETYEATLTSLLKHERNV"
     gene            746..1348
                     /locus_tag="VN478_06040"
     CDS             746..1348
                     /locus_tag="VN478_06040"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HWQ22267.1"
                     /translation="MTRIHRNGISKLTILIGTLVVLLIAVIIVSAMQEVKRTTPDPMA
                     TGPSTLSTGYVLRPFSASDLTGHTVDNTFFKKQRLTMVNVWGTFCGPCIREMPDLALL
                     PAEFRASEFAILGIVADTPDTSNEDTARQLTGSTGVTYTNVIPDASIETELLADVSVV
                     PTTFFVDRTGKVVGEVLTGSHTKAEWIDIIQGVLANLTTR"
     gene            1356..1499
                     /locus_tag="VN478_06045"
     CDS             1356..1499
                     /locus_tag="VN478_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004608606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1871A family CXXC motif-containing protein"
                     /protein_id="HWQ22268.1"
                     /translation="MSTRVRILRVALLAAALLGIVVGAMSGEVRTVLTKAINVCLQCI
                     GIG"
     gene            1504..2385
                     /locus_tag="VN478_06050"
     CDS             1504..2385
                     /locus_tag="VN478_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016297493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HWQ22269.1"
                     /translation="MSVRRTGFQAVTAVLQNSWFAGFLHGRIWRGSSKAVCVPGLNCY
                     SCPGAWGSCPIGSLQAVFNRTTDRISLYVAGTIGLVGVMLGRLVCGWLCPFGLIQELL
                     YRIRSRKVRVGPSRLRFLKYGVLAVLVVLMPLLVRDRFGIGDPWFCKLVCPAGTLEAA
                     VPLLALNASLRDAAGWLFAWKASILAVTVAASVLVYRPFCRFVCPLGAVYGLFNRVSF
                     VQLTLDEHKCIHCGMCADACRMGVDPSVHAVDPECIRCSDCVAVCPMHALSLGLPSGQ
                     ARRGAVTDSRSVPDNSH"
     gene            complement(2382..3872)
                     /locus_tag="VN478_06055"
     CDS             complement(2382..3872)
                     /locus_tag="VN478_06055"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HWQ22270.1"
                     /translation="MTTPESNRHVSSVVRDELNTLWQNVLDNVASTRRVSSEQFPFLL
                     PDDAIARMEGDEGWSSYVRKRLFLEYAIDAGRRAGRPLDAFVRELDHTVRALMPWTQR
                     WQAQVDAGAAPVLQTLDLEVPESEQLSLIRSNGAVLFPGEDVPFAGRRLLLSPGRTRI
                     DEGVLRASAASFESWSEVERADLECRDGLMPLFGTEHAGIVVVPGAASLGMEVVVVAA
                     SPPGAVVMVLRHGPQGDRIAHVVSRRGRVVDVLRAQADGIVDLQTLRARLTDTRPSTV
                     IVSQVDADSGAVAPLDDYAAVIHEVAPQSLLVVDGTWATGCMPQRMDDWDADMVFTDS
                     GSALGGCAGLVLAAVSKRLMDRKVRHDAAVPLYIELSRWSSPTGAEVPPSLVFALRAA
                     LQRIYAEGPPARFARCDGIAREFRDKAAVHGFGILAAPGHEASSISALNVPDGVDAAE
                     LCGALERRGIDVGLISTGPVVAHTGDVSAEDLERFWLAVEALQLQA"
     gene            complement(3889..>5103)
                     /locus_tag="VN478_06060"
     CDS             complement(3889..>5103)
                     /locus_tag="VN478_06060"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWQ22271.1"
                     /translation="AMTLSVLAFCGFAPTILLMPLAGVLVDRWDRKVTMAVSDAASGC
                     ATVAVLLLFVTGRLQVWHLYLTSIFSASFQAFQWPAYSAAISTMIPRQHYARASGMLS
                     LAQSASGILGPAVAGTLVASSGLPTVLVADIVSCIAAIGVLFFVQIPRPLPSAEGLRA
                     RGAWWRQIMFGFRYIFARPPLLSLMLLFIVFNLLAMLCHGVWSALLLARSGGAATVLG
                     FTESASAVGFGLGGLLLIIWKGPARKVSGILWAMVAASVVGSVLVGVGRSLAVWAVAG
                     FVGSMTMPVMNGLSQSIWQCKVPADVQGRVFSARMQLSQVVTPVAMIASGAMADRVFE
                     PAMMRGGLLARLFTPVVGVGPGAGMAVMFIVFGILGTVAAVIGFFVPQLRNAEELLPD
                     AVTLDAACGVRQ"
BASE COUNT          999 a         1679 c         1553 g          872 t
ORIGIN      
        1 tgccaggagg ggcagagtat gatgaaggac agacagggag aggaggctcg cgccggatgc
       61 acatcaacac caatggggac cctggcagaa tcactcttga gctcggggac atcacgcggt
      121 tcaggggcga cgtcatcgtc aacgccgcca acgagtcact gctcggcggt ggaggggtcg
      181 acggagccat ccacgcggcc gcagggcccg ccctgctggc cgaatgccgc accctgcacg
      241 gatgcgcaac cggccgggca gagataacgg gcgggtatca tctgcccgca cggtatgtca
      301 ttcatacggt cggcccggtg tggcacggcg gagcgcgggg tgaggaagag ttgcttgaga
      361 gcgcatatcg gtcctgtctc gaactggctc agcggcacga cctgcgcact atcgctttcc
      421 cggcgatcag caccggcgcg tacgggtatc ctctggagaa ggcgactcgt gtcgccctgc
      481 ggaccatcct cacgtggctg gatgaacacg ttctgcctga acacgtcatc ttcgtctgct
      541 atgaccagcg cacgctggaa acgtacgaag ccacgctcac atcgctgctc aaacatgagc
      601 gcaacgtctg accaaaacat cggttcgcct gtttcgcact gcgtgctcat ccgtcattgt
      661 caagagactg tattgacttc tcctgttgtg cacggggcga gaccgctgta tcatgcttgc
      721 gacatcccag tgaggtgaac gtgacatgac tcggatccac agaaacggaa tcagcaagct
      781 gaccatcctg atcgggactc tcgtggtgct ccttatcgcc gtcatcatcg tgtccgccat
      841 gcaggaggtg aaacgcacga cacccgaccc aatggcaacc ggtcccagca cactgagtac
      901 ggggtatgtg cttcggccct tcagcgccag cgacctgaca ggacacacgg tcgacaacac
      961 cttcttcaag aagcagaggc tcaccatggt caacgtctgg ggaacattct gcggtccctg
     1021 cattcgcgag atgcccgatc tggctctgct cccggccgaa ttccgtgcca gtgaatttgc
     1081 catcctgggc atcgtcgctg acacccccga cacgtccaac gaggacacag cacgccagtt
     1141 gacaggatcg accggggtca cgtacacgaa cgtcattcct gacgcctcca tcgagaccga
     1201 gctgctcgcc gacgtcagtg tcgtgccaac cacgttcttc gtggaccgca caggcaaggt
     1261 cgtgggagaa gtgctgacgg gctcgcacac caaggccgag tggatagaca tcatccaggg
     1321 cgtcctcgca aacctcacca ccaggtagca gcccagtgag cacacgtgtc aggatcctgc
     1381 gtgttgctct tctcgctgca gccctgctcg gcatcgtcgt cggagccatg agcggcgagg
     1441 tccgcacggt gctcaccaag gccatcaacg tgtgcctgca gtgcatcggc attgggtaga
     1501 cccatgagtg tacgcaggac cggattccag gccgtcaccg ccgtcctcca gaactcgtgg
     1561 ttcgccgggt tcctccacgg ccgcatctgg cgcggttcca gcaaggccgt ctgtgtcccg
     1621 gggctcaact gctactcgtg cccgggtgcc tggggctcgt gccccatcgg ttcgctgcag
     1681 gcagtcttca atcgcacgac tgaccgcata tctctgtatg ttgcaggaac cataggtctc
     1741 gtgggtgtca tgctggggcg cctggtgtgc gggtggctgt gtcccttcgg cctcatccag
     1801 gagctgctgt accggatccg ttcgcgcaag gtgcgcgtcg gtccctcacg actgaggttc
     1861 ctcaagtatg gcgtgcttgc cgtcctcgtc gtcctgatgc cgctgctggt tcgagaccgg
     1921 ttcggtatcg gtgacccatg gttctgcaag ctggtgtgcc ccgccggcac gctggaggcc
     1981 gcagtgcccc ttttggctct gaacgcatca ctgcgagacg ccgcaggatg gctgtttgcc
     2041 tggaaggcga gcatcctggc tgtgacggtc gcagcgtcgg ttctcgtata ccgcccgttc
     2101 tgcaggttcg tctgcccact gggggccgtc tatggactct tcaatcgggt gagcttcgtt
     2161 cagttgacgc tggacgagca caagtgcata cactgcggca tgtgcgccga cgcgtgcagg
     2221 atgggcgtgg acccctcagt tcatgctgtc gatccggagt gcatccggtg cagcgactgc
     2281 gtcgccgtct gtccaatgca tgccctgtcc ctgggactgc cgtccggtca agccagacgg
     2341 ggtgccgtca cagattcgcg cagcgtgcct gacaactcgc actaggcctg cagctgaagc
     2401 gcctccaccg cgagccagaa acgctcaagg tcctcggcac tgacgtcgcc cgtgtgggcc
     2461 acgaccgggc ccgtcgatat cagccccacg tcgatgcccc gacgttccag cgcaccgcac
     2521 agctcagcgg cgtcgacacc atcgggtacg ttcagagccg atatggacga tgcctcgtgc
     2581 cctggtgcgg ccaggatgcc gaacccatgg actgcggcct tgtccctgaa ttcccgagcg
     2641 atcccgtcac accgtgcaaa ccgggccgga ggtccttcgg cgtagatccg ctgcagagca
     2701 gcgcgcagcg cgaagaccag gctggggggc acctcggcgc ccgtcggcga agaccaccgg
     2761 gacagttcga tgtaaagcgg aactgcagca tcatgccgca ctttgcgatc cataagacgc
     2821 ttgctcacgg ctgccagcac caggcctgcg caccccccga gcgccgagcc gctgtcagtg
     2881 aaaaccatgt cggcgtccca gtcatccatg cgctgcggca tgcagcctgt ggcccacgtc
     2941 ccgtcgacga ccagcaggga ttgcggggcg acctcatgga tgactgctgc gtagtcgtca
     3001 aggggagcga cggcgccgct gtcagcatcc acctgtgaca cgatgactgt ggacggtcgc
     3061 gtgtcggtca ggcgtgcccg cagggtctgc aggtcgacga tgccgtccgc ctgcgcacgc
     3121 aggacatcaa cgacgcggcc tcgacgcgac accacgtgcg cgatcctgtc accctgaggc
     3181 ccatgccgga ggaccatgac cactgctccc ggaggagacg ctgcgaccac gactacttcc
     3241 ataccgaggg acgctgctcc cggtacgacg acgataccgg catgttcggt cccgaacagc
     3301 ggcatgagtc cgtcccgaca ctccaagtct gcccgttcca cctcgctcca gctttcaaag
     3361 gacgcggcag aggctcgcag gacaccctca tcgatgcgtg tccgacccgg cgaaagcagg
     3421 aggcgtctgc cggcgaacgg cacgtcctcg ccggggaaca ggacggctcc attggaccga
     3481 atcaagctta gctgttcgct ctcgggaacc tcgagatcga gcgtctgcag gaccggcgcg
     3541 gcccctgcgt cgacctgggc ctgccagcgc tgtgtccagg gcatgagcgc acgcaccgta
     3601 tggtcgagtt cacgcacgaa agcatccagc gggcggcccg ctcgtctgcc ggcatcgatg
     3661 gcgtactcca ggaagaggcg ttttcggaca taggatgacc acccttcgtc gccctccatc
     3721 cgcgcgatag cgtcgtccgg caacaggaaa ggaaactgct cagatgagac cctgcgagtc
     3781 gatgccacgt tgtccagaac attctgccac agcgtgttca actcgtcccg gacgaccgaa
     3841 gacacgtgcc gattcgactc tggtgtcgtc attgctatcc tccctttgtc actgcctcac
     3901 cccgcacgcc gcgtccagcg tgacagcgtc cggcagcaac tcttcagcgt tccgcagctg
     3961 tggaacgaag aatccgatca cagctgcaac agttcccagt ataccaaata caatgaacat
     4021 cacggccatc cccgctcctg ggccaacgcc gaccaccggt gtgaacagcc tggccagcag
     4081 tccccctcgc atcatagccg gttcgaacac acgatctgcc atcgctcccg aagcaatcat
     4141 ggctacaggc gtgacgacct gtgacagctg catgcgcgcc gagaacacac gtccctggac
     4201 gtcggccggg accttgcact gccagatgga ctgactgagt ccattcatga cgggcatcgt
     4261 catcgatccg acgaatccag cgaccgccca cacggccaat gaccttccca cgcccacgag
     4321 gacactgccc actaccgatg ccgccaccat cgcccacagg atgccagata ccttgcgcgc
     4381 gggacccttc cagatgatga gcagcagacc acccaggcca aaaccaactg cagaggcaga
     4441 ctcggtgaac cccaggactg tggccgcgcc ccctgagcgg gccaggagca gggcgctcca
     4501 gaccccgtga caaagcatgg cgagcaagtt gaatacgatg aagagcagca tcagcgacag
     4561 cagcggcggc cgggcgaaga tatagcggaa cccaaacatg atctgtctcc accacgcccc
     4621 gcgcgcccgc aagccctcgg cagacggcag ggggcgggga atctgcacga agaacagcac
     4681 cccgatggcg gcgatgcacg acacgatgtc ggcaaccagg acagtaggca gtccggaaga
     4741 ggccaccagc gtgcccgcaa cagccggtcc caggatgcct gaggctgact gagccagcga
     4801 cagcatgccg gaggcccggg catagtgttg cctggggatc atcgtgctga tggcggctga
     4861 ataggccggc cactggaacg cctgaaacga tgccgagaag atactggtca ggtacaggtg
     4921 ccagacttgc agtcggccgg tcacaaagag cagcaggacg gcgaccgtgg cacagccgga
     4981 ggctgcgtcg cttactgcca tggtcacctt gcggtcccag cggtcgacga ggacgccggc
     5041 aagcggcatg agcaggatgg taggggcgaa tccgcagaag gcgagcacag acagggtcat
     5101 cgc
//
LOCUS       DATMZD010000291         8022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_28671,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000291 DATMZD010000000
VERSION     DATMZD010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8022
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28671"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1151)
                     /locus_tag="VN478_06065"
     CDS             complement(<1..1151)
                     /locus_tag="VN478_06065"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWQ22272.1"
                     /translation="MSRIQKALLAISGGHFLVEWYAAFLPPLVPVFRTRLGFSLGAAA
                     ALGASLPIVSGLVQTIFGLLSDRMRHANILVVTSSILAAVSLALLPFARSFGWLLLIF
                     TVIALSLAMFHPQGAAGTSQLSSTNKGRFMSVFNFGGSMGSFAGSLAVIPLLGLLHLE
                     RFWVLAVPGIALAVFQVFALPRREAMDVDAKNGNGGRMTDSPTFKGYLVLVANSTLTA
                     MVYNGVTILLPLLFQELGFLPGKAGVYLAVGSLVGAIANVIGAELSDHIGRRTINIIG
                     AAGVAVTLFMFVLTGSTSIFWSPAMTFFACFTLSSNIVFAHELVTNHRGLVSASIMGF
                     SWGVGGLVVILLGQWAQHTSITSAYRLLVGVAAVLVALAFLLPTRHAIRA"
     gene            complement(1409..1924)
                     /locus_tag="VN478_06070"
     CDS             complement(1409..1924)
                     /locus_tag="VN478_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22273.1"
                     /translation="MDDPEVETVLLACQAVERQGTFKSLERTLMITRSFVVLAPFDQA
                     KVRTLRESRAAEAKAQGASRFQQLWYAAQAPFALVDGYLGMTLASVLTELPTARVLGY
                     ADIEEVDLIQGAPPGRFGARTAGEAPHKLALTTAAGEIALLVDGHTDPRELRRLLGGV
                     AGDRLVADQRG"
     gene            complement(1924..2178)
                     /locus_tag="VN478_06075"
     CDS             complement(1924..2178)
                     /locus_tag="VN478_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22274.1"
                     /translation="MSKRWVSELLVAIVALVCTAAMAALFWRFGSRTDYWGLLVPALF
                     LLFYISWKSATRAKESVSAIPYSVLMVLVSALVLILDLRF"
     gene            2364..2543
                     /locus_tag="VN478_06080"
     CDS             2364..2543
                     /locus_tag="VN478_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22275.1"
                     /translation="MELILVPFGIVIAALVVAGAVTGAVFRFVWIVLKVIFVVCFWWL
                     LLLVGGVALLRSFVR"
     gene            2752..3174
                     /locus_tag="VN478_06085"
     CDS             2752..3174
                     /locus_tag="VN478_06085"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWQ22276.1"
                     /translation="MKKMKNGKRMELVVLRLHNYWDAQDPRRVGGLTASQLSLLLSLS
                     EHPGCRVQDTAERLDLTAPTISIGVRRLEKMGLVQRDEDPEDQRAVCLYLSPQGRKVA
                     SKARALRAAKLETILGALSVEEQESLLEMLERAVPEIG"
     gene            3215..4390
                     /locus_tag="VN478_06090"
     CDS             3215..4390
                     /locus_tag="VN478_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012662457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HWQ22277.1"
                     /translation="MNSRPIVPGITLMGAVDWDRKLFDSLIPLPEGTSYNAYLVQGSE
                     KTALVDTVDPAKLDVLMDQLDAVDHLDYLVAQHAEQDHSGSIPAVLARYPEAVLVTNP
                     KAKELLQEHLDIAVDRFRTVADGETLSLGDKTLRFIYLPWVHWPETMGTYVEEDRMLL
                     SCDFLGSHRAQSRVFAERNIVMHGAKRYYAEIMMPFRGLIIKNLDKLAGMQIDMIGPS
                     HGPVYNDPAFILDAYREWAGGPPHNKVVIPFISMHGSTLKMVDRLTADLGDRGVDVER
                     FDLTTADLGDIAMSLVDAATIVIGTPAVLTGPHPKTIEIAYLAGALRPKTQFVSIIGS
                     FGWGQRIVDVLGGLIAPLKAELLTPVLAKGDPRAADMQAIDALADTIAAKHHEAGLL"
     gene            complement(4497..6242)
                     /locus_tag="VN478_06095"
     CDS             complement(4497..6242)
                     /locus_tag="VN478_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22278.1"
                     /translation="MRKSFVIALQAIIVILLTIAMSSLPVLAATGAARLVSDNETVYV
                     LMNNDGSSQKTILMDWLRVEGTGTYDFRDPVPGAQDVRLVDGSGILAFEGGSAHITGT
                     SSGLEDIYYRATLAKSLPVDVRVTTTLGGKAIAYADLKKQAGDVTVTIGLTNNEKQGS
                     VYVPWTCSLTLTLDNADVRSIDAGTDGVTVVTGSRTIVTYVVVLDASAQVTLRYTALR
                     GTDPNLQLAIRPSMPDFPLPSASSLVPLKDGLSQIALAFDGQKSMLDGVIKALDSQMV
                     PTFDVTQLDRLNDLVTLLKADEAVLRGVYASIDTTQLESLSALTLGITQLQTAISGVT
                     QGVGQLAGALMVQAQLVDKALALNASMEQATAGLPAEAGGTLSALILAQRSLLETVAY
                     GGTIQGVPGPIPGLGTIVENLTKLQSGLQGVSGGFDKLAQGLGQLPRLATGMSTLRTT
                     IGTVLDGGSLAGQTLPPFATASSAIAQVPAAVRSQVQTLTSKSRQSVTQLVQTLTLLR
                     DGGAIGGRTMPSLMKLKLGVLSMKNGIGGFAATIQEQVDLMAVKKTLASKWTSFGGRT
                     PGSAGSVMFVLTMAQ"
     gene            complement(6239..>8022)
                     /locus_tag="VN478_06100"
     CDS             complement(6239..>8022)
                     /locus_tag="VN478_06100"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HWQ22279.1"
                     /translation="TWAKSVADPVTPAEVAPEAASEWIQKDAYRVELTFSTSDDKMGS
                     TVTALRERAGTDGIVLATSEITRSLQESSISSTGLYFLFGALLVLVFLALYFPDPLTP
                     VFMLIVMMGSVVVNLGTAALLGRSLFFISLTVVSVLQLAVTYDYGLFLYHRFAEERVT
                     RSPEQAMVMALVRTFKAVLLSALTTIAGFLALTFGHLTLIKEMGWLLLQGVTLSFLFT
                     LVFLPAFLLTFDRVSHLRKFNLQSWLTRASVAVGRAVSRAPLIVVLILCLLVPAAWFM
                     HRTVVQTFNRQDYMPPTASFSIAQTEYEKYFGNRDTMYVVLDRLSDHTALLRRIQALP
                     SVKEVLSPGTMVEPSIPQELLPSDVVRQFTGSKYTLAIVTSAYGIDDPRNDQLKVDIA
                     RLMEDVPGEHYLTGTSLITDDLKKAGQADLGRTTWITVLLIGLLLLAGFRSLSLPALL
                     VLVIETAIWLNLSWYALVGATITFLVPIVLNAIQLGSTIDYSVLTATRFEEETTAGGT
                     RNVESTIRTSLPSVVVSAGTFILMALPSAVLSNVPVIKQIMSSLVRGAAISAIMVIVF
                     VPALLQVLHKPIAWTSIGFKARKEKNK"
BASE COUNT         1685 a         2301 c         2539 g         1497 t
ORIGIN      
        1 gccctgatcg cgtgccgggt gggcaggagg aatgcgagcg cgacgaggac ggccgcgaca
       61 ccaacgagca ggcgataggc tgatgtgatg ctcgtgtgct gggcccactg gcccaggaga
      121 atgaccacga ggccgccgac accccaggag aagcccatga tggatgcgga gacgagccca
      181 cggtggttcg tgaccagttc gtgcgcgaag acgatgttgg agctcagggt gaaacaggcg
      241 aaaaaggtca tggccgggga ccagaagatg ctggtcgaac cggtcaggac gaacatgaag
      301 agggtcaccg ctacgccggc tgccccgatg atgttgatcg tcctgcggcc gatgtgatcg
      361 gagagttcgg cgccgatgac attggcgatt gcgcccacca gcgaaccgac ggctagatag
      421 acgccagcct tccccggcaa gaacccgagt tcctggaaca gcagaggcag caggatggtg
      481 acgccgttgt agaccatcgc cgtgagtgta gagttggcga cgagcacgag gtatcccttg
      541 aatgtcggac tgtccgtcat gcggccgccg ttgccattct tggcatctac gtccatggct
      601 tcccggcgag ggagcgcgaa cacctggaag acagccagcg cgatgccggg gacggcgagg
      661 acccagaagc gctcgaggtg cagcagtcca aggaggggga tgactgcaag ggagccggca
      721 aacgaaccca tggacccgcc gaaattgaag acggacatga agcgaccctt gttcgtcgag
      781 gacagttggc tcgtaccggc ggcgccctgc ggatggaaca tcgcgagtga gagagcgatg
      841 accgtgaaga tgagcaggag ccagccgaag ctgcgggcga acggcagcag cgccagcgaa
      901 accgcggcga ggatggaaga ggtgacgaca aggatatttg cgtgccgcat gcggtccgag
      961 agaaggccga agatcgtctg gacaagaccc gagacgatcg gaaggctggc tccgagcgcg
     1021 gcagcagcac ccagtgagaa gcccaggcga gtacggaaga cagggacgag agggggaagg
     1081 aaggcggcat accattcgac gaggaagtga ccaccggaaa tcgcgagcag tgctttctgt
     1141 atgcggctca ttggtttact ctctcctgga catgcgaaaa aggcaacctc ggagcgagca
     1201 gacgagtccg ccatgaacgc aagcaatgtt ccgcgcgagg ctaccttctt ctgttattag
     1261 tatagcaaac ctttgagcta tgtcaatatc cgaaataact caggctgcat gccactccca
     1321 gacatcggta acgcagggag catcaccaga actcgtgctc agaggtacga ggattggcgt
     1381 ggcagttcgg cgttgacggt ccagtcgctc aacctcgctg gtcagcaacc agcctgtctc
     1441 cagcgacgcc cccgagcagc cggcgcagtt cgcgcgggtc cgtatgaccg tcgaccagca
     1501 gggcgatctc gccggcagcc gtggtgagtg ccagcttgtg tggagcctct cctgctgtcc
     1561 gtgctccaaa gcgccctgga ggcgcaccct ggataaggtc aacctcctcg atgtcggcgt
     1621 agcccagcac gcgagctgtg ggcagctccg tcaggacgct ggcgagagtc atcccaagat
     1681 atccgtcgac aagggcaaag ggagcctggg ccgcgtacca cagctgctga aaacggcttg
     1741 ctccctgggc tttcgcctca gcggccctcg actcacggag tgtacgtact ttcgcctggt
     1801 caaatggggc gagcacgaca aacgacctgg tgatcatcaa ggtgcgctcg agggacttga
     1861 aagttccctg ccgttcgacg gcctgacagg cgagaaggac ggtctctact tccgggtcat
     1921 ccatcagaac ctcaggtcca ggatgagcac gagtgccgaa acgagaacca tgagcacaga
     1981 atagggaatg gcggacacag actcctttgc ccgggtcgct gacttccagc tgatgtagaa
     2041 caggaggaag agtgcaggca cgaggaggcc ccaatagtcc gtgcgcgagc cgaaacgcca
     2101 gaacagggcg gccatggccg ccgtgcagac gagggcgaca attgccacca gtagttcact
     2161 gacccatcgc ttgctcacct cgaccccctt tcagcaatgt cccaggaatg cgtatgcaga
     2221 ctcaggatag tccaaggcag cgcagggtca atgacgggca caactgcacc ggcggtggcg
     2281 gtcgagcgag aggcgcctcc tgaacaggcg gatgcgtaac gttcggccgg ggagaccgta
     2341 gacaagtcgt ggaggtgatc cctgtggaac tgatccttgt accatttggc atcgtgattg
     2401 cagcacttgt cgtggcggga gccgttacgg gagccgtgtt caggttcgtg tggattgtgc
     2461 tgaaggtcat ctttgttgtg tgcttctggt ggcttctcct tcttgtggga ggggttgcgt
     2521 tactgaggtc gttcgtgcgc tagtcggtct gccgctggca ggtcacaggt caaggcatcc
     2581 ggcttgtccg ggtgccttga tgcatttctt gtcacacaaa cagatgacga ctctgcaaac
     2641 gctgtcaaca tggagtacac agcgtctttc tcctttatat ctttacacga gaccattgac
     2701 ggccaaagaa agcactctag gattaggtta tcaaagtaag ggggaaccga gatgaaaaag
     2761 atgaagaatg gcaagcgtat ggaattggtt gtactgagac ttcacaacta ctgggatgct
     2821 caggatccgc gccgagtcgg cgggctgacg gcatcgcagc tttcgctgct gctgtcgttg
     2881 tccgagcatc caggatgccg ggtacaggac acggccgaac ggctggatct gactgctccg
     2941 accatcagta ttggtgtccg caggctggag aagatgggtc tcgtgcagcg cgacgaggac
     3001 cctgaggacc agcgggccgt ctgtctgtac ctttctccac agggcagaaa ggtcgcgtcc
     3061 aaggcacgtg ctctgcgtgc agccaagctg gagacgattc ttggcgcgct gtcggtggag
     3121 gagcaggagt ctctccttga gatgctggag agagccgttc cagaaatcgg ctgaaccatt
     3181 gatacagtag cctgttgatg aggaggaggt taccgtgaat tcacgcccta tagttccagg
     3241 catcaccctc atgggagcgg tcgactggga tcgcaagttg ttcgattcgc tgattccact
     3301 gcctgaaggg accagctaca acgcgtatct ggtgcagggc agtgagaaga ccgcgctggt
     3361 cgacacggtc gacccggcca agcttgacgt cctgatggac cagcttgacg cagtcgatca
     3421 cctggactac ctcgttgcac agcatgccga gcaggatcac agcggttcta tccctgctgt
     3481 ccttgcccga tatcccgagg ctgtcctcgt gacaaacccg aaggcgaagg aattgctgca
     3541 agagcatctg gacatagctg tcgaccggtt caggactgtc gcggacggtg aaacgctgtc
     3601 actgggcgac aagaccctgc ggttcatcta cctgccatgg gtccactggc ccgagacgat
     3661 gggcacatac gtcgaggaag accgtatgct cctctcgtgc gatttcctgg gttcgcacag
     3721 ggcgcagagc cgcgtgttcg ccgagcgcaa catcgtgatg cacggtgcca agcgctacta
     3781 cgcggagatc atgatgccgt ttcggggcct catcatcaag aatctcgaca aactggccgg
     3841 aatgcagatc gacatgatcg gccccagcca cggtcctgtc tacaacgacc ccgccttcat
     3901 cctcgacgca taccgcgaat gggcgggtgg cccaccacac aacaaggtcg tcatcccctt
     3961 catctccatg catgggagta cgctgaagat ggtcgatcga ctgacggccg acctcggtga
     4021 caggggcgtc gacgtcgaac ggttcgacct gacgactgcc gatctgggcg acatcgccat
     4081 gtccctcgta gacgctgcga cgatcgtcat cggtacgccg gcggtgctga ccggaccgca
     4141 ccccaagacc attgagatcg cctacctggc cggagctctc cgtcccaaga cgcagttcgt
     4201 gtcgatcatc gggtcatttg gatggggtca gcgcatcgtc gacgtcctcg gcggactcat
     4261 cgcgcctctc aaggcagaac tccttacgcc cgtcctcgcc aaaggcgacc ctcgtgccgc
     4321 cgacatgcag gcgatcgacg ctctggctga tacgattgcc gccaagcacc acgaggctgg
     4381 cctgctctag actctgttcc cccctcttgc acaacagacc cccggcctca cggccggggg
     4441 tctctgctac tggaccaggc ttgttcagat ccggagtggg cggatcgccg ccgccgctat
     4501 tgcgccatcg tgagcacgaa catgactgaa ccggcactgc cgggcgtcct gccgccaaaa
     4561 gaggtccact tcgaggcaag tgttttcttg acggccatga ggtcgacctg ctcctggatc
     4621 gtggctgcaa aacctccgat accgttcttc atggaaagca cgcccagttt gagcttcatg
     4681 agcgacggca tggtgcgccc acctatcgct cctccatctc tcagaagggt cagtgtctgg
     4741 acgagttgtg tgaccgactg cctgctcttg ctggtcagcg tctgcacctg tgaccgcaca
     4801 gcggcgggta cctgggcgat agcgctggag gctgtggcga agggcggcag cgtctgaccg
     4861 gcgagggacc cgccgtcgag caccgtgcct atcgtcgtgc gaagggtgga catacccgtc
     4921 gcaaggcgcg gaagctgacc gagtccctgg gcaagcttgt cgaatcctcc ggaaacgccc
     4981 tgaaggcctg attgcagctt ggtcagattc tcgacgattg tcccgagccc cggaatggga
     5041 ccgggtactc cctgaatcgt cccgccatat gcaaccgtct cgagcaacga tcgttgtgca
     5101 aggatgagtg cgctgagcgt gccgccggct tccgccggca gtccagcggt cgcctgttcc
     5161 atactggcgt tgagggccag ggccttgtcc accagctgcg cctgcaccat cagggctccc
     5221 gctagctgac caacaccctg agtgacgcca ctgatcgccg tctgaagctg tgttataccg
     5281 agtgtgaggg ccgacagaga ttccagctgt gtcgtgtcga tgcttgcgta gactccacgc
     5341 aggacagcct cgtctgcctt cagaagcgtc acgaggtcat tcagacggtc cagctgcgtg
     5401 acatcgaatg tcggcaccat ctgtgagtcg agagccttga tgacgccgtc gagcatcgac
     5461 ttctgtccgt caaaggcaag ggcgatctga ctcagtccat ccttcagggg aacgagactg
     5521 cttgccgagg gcagggggaa gtccggcatc gacggacgta ttgcgagctg caggttgggg
     5581 tccgtcccgc gaagcgccgt ataccgaagg gtgacctgcg ccgacgcgtc caggaccacc
     5641 acgtacgtga cgatggtacg actgcccgtc acgacggtga caccgtccgt tccggcgtcg
     5701 atggaacgca catccgcgtt gtcgagtgtg agcgtcagcg aacatgtcca tggcacgtac
     5761 accgacccct gcttctcgtt gttagtgaga ccgatggtga ccgtgacatc ccccgcctgc
     5821 ttcttcaggt ccgcgtatgc gattgccttg ccgccgagtg tcgtcgtgac gcgcacgtcg
     5881 accggaagag acttggcgag tgtagcacga tagtagatgt cctcgagacc tgaggacgtc
     5941 ccggttatgt gggcggagcc tccctcgaaa gcaaggatgc cgctcccgtc cacaagccgg
     6001 acgtcctggg ctcccggcac cggatcgcgg aagtcatatg tgcctgttcc ctccacgcgg
     6061 agccagtcca tcaggatagt cttctgtgac gatccatcat tgttcatcag gacgtagaca
     6121 gtctcattgt cagacacgag tcgcgctgcc ccagtagcgg caagaaccgg cagcgacgac
     6181 atcgcgatcg tgagcaagat gacgatgatc gcctggagag cgataacgaa tgactttctc
     6241 atttgttctt ctcctttctg gctttgaagc caatggatgt ccacgcgatg ggcttgtgca
     6301 gcacttgaag cagagcgggc acgaagacga tgaccatgat cgccgagatg gctgctcccc
     6361 ggacaagaga ggacatgatc tgcttgatga cagggacgtt cgacaggact gctgacggga
     6421 gagccatcag gatgaaggtg ccggcgctca cgacaacgga gggcagtgat gtgcggattg
     6481 tgctctccac gttgcgggtc ccgcctgccg tcgtttcttc ctcgaagcgc gtcgctgtca
     6541 gtacagagta gtcgatggtg gagcccagct ggatggcgtt gaggacgatt ggaacgagga
     6601 acgtgatagt ggcgccgacc agtgcatacc atgagaggtt cagccagatg gccgtctcga
     6661 tgaccagcac caggagcgca gggagactga ggcttcggaa gcctgcgagc aggaggagac
     6721 cgatgagcag gaccgtgatc cacgtcgtcc gccccaggtc cgcctgcccc gctttcttga
     6781 ggtcgtcagt gataagtgat gtccctgtca ggtagtgttc gccggggacg tcttccatga
     6841 gccgggcaat gtcgaccttg agctgatcgt tgcgcggatc gtcgatcccg tatgcgctgg
     6901 taacgattgc cagtgtgtac ttcgatccgg tgaactgccg gacgacatcg ctgggcagga
     6961 gctcctgggg gatgcttggc tcgaccatgg tcccagggct cagtacttct ttgacactcg
     7021 gaagagcctg gatccgacgc aggagggcag tgtgatcgct gagccggtcc aacacgacat
     7081 acatggtgtc ccggtttccg aagtacttct catattcggt ctgcgcgatc gagaatgagg
     7141 cagtgggggg catgtagtcc tggcgattga aggtctgaac aaccgtccgg tgcatgaacc
     7201 aagctgccgg cacgagcagg cagaggatga ggacgacgat gagaggagcc ctggacacgg
     7261 cccttccgac agcaacgctt gcgcgggtca gccagctctg caagttgaac ttccgcagat
     7321 ggctgacccg atcgaacgtc agaagaaatg ccgggaggaa gaccagcgtg aacaggaaac
     7381 tgagtgtcac gccctgaagg agcagccagc ccatttcctt gatcagagtg agatggccaa
     7441 acgtgagggc gagaaacccc gcgatggtcg tgagcgccga caggagtacc gccttgaacg
     7501 tacgtacgag tgccatgacc atggcttgtt cgggagaccg tgtgacccgc tcttccgcaa
     7561 agcgatgata gagaaacagg ccgtagtcgt aggtcacagc cagctgcagc actgacacga
     7621 cggtgaggct gatgaagaag aggctccgcc cgaggagagc ggcggtcccc aggttgacga
     7681 cgactgaccc catcatgacg attaacatga agacgggcgt gagggggtcg gggaagtaaa
     7741 gagccaggaa gaccaggacc agcagtgccc cgaacaggaa gtagagtcct gtggagctga
     7801 tagaggactc ctgcagactc ctggtgatct ccgaagtcgc cagaacgatg ccgtctgtgc
     7861 cggcgcgctc tcgaagcgcc gtaaccgtgg aacccatctt gtcgtccgag gtcgagaagg
     7921 tcagctcgac tcggtatgcg tccttctgga tccactccga tgccgcttca ggggctacct
     7981 ccgcgggcgt gacagggtca gcgacactct tcgcccatgt ac
//
LOCUS       DATMZD010000292         2440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_109243,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000292 DATMZD010000000
VERSION     DATMZD010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109243"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..294)
                     /locus_tag="VN478_06105"
     CDS             complement(34..294)
                     /locus_tag="VN478_06105"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HWQ22280.1"
                     /translation="MDTKLIAITTASDEMEANMIVGLFAAAGIPAMAKRSVKDGYGSI
                     YQGPFGSFDILIDEENATAAQTVLDAEDVPAELDEKPDIPAD"
     gene            484..1926
                     /gene="gltX"
                     /locus_tag="VN478_06110"
     CDS             484..1926
                     /gene="gltX"
                     /locus_tag="VN478_06110"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HWQ22281.1"
                     /translation="MVRVRYAPSPTGHIHVGNARTAIFNYLYARHEGGTFIMRLDDTD
                     LERSTEENIDSMLDDIRWLGLDWDEGFLKGGDLGPYRETERKPLYDKYLQQLLDEDKA
                     YRCFCTRDEVEAERRQAEAEHRIYRYGGTCAHLSADQVAGNIAEGKPYVIRLRIPVED
                     VVVHDLIRGDVQFKSEEFDDFVIARQNGIPIYNFATVIDDALMNITHIIRAEEHLSNT
                     PRQVFIYRALGFDVPEFAHVSLILAPDHSKLSKRHGAVSVGEYRLEGYLPEALINYLV
                     LLGWSPKDDREFFTVDELIKEFSLAGVGKSGAVFDKKKLTWMNHMYLGRKDAADTLAI
                     LKKDFAKEYDAAFGGLPEDKTLAAIDLTKGNAQVLPDLFRLARNIVQAAVSITDEKSR
                     EVIGSTEAKQVLQYLVEHFDAIPFGQGDDTMSAWVEDLRNQIPLPAKKLYSPIRVALF
                     GSKDGPEIVKLFLMLEPASIRERLQKALAL"
     gene            complement(2048..>2440)
                     /locus_tag="VN478_06115"
     CDS             complement(2048..>2440)
                     /locus_tag="VN478_06115"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: protein motif:HMM:NF016438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HWQ22282.1"
                     /translation="LLIDGIGDTIRYSISGDPVLEVDAAWTLLAGLDLRRHGAQIVSC
                     PTCGRCEVPDILSLVAQLEERLSEIVKPVTVALMGCVVNGVGEGKAADVGIACGRDLG
                     VLFIRGEPVRNVKPCDFIDTIIAEANRL"
BASE COUNT          521 a          744 c          698 g          477 t
ORIGIN      
        1 cccaggagta cggccgccag atcagtgcac cggtcagtcc gcgggaatgt ccggcttctc
       61 gtcgagttcg gcgggtacgt cctcggcgtc cagcacagtc tgtgctgctg tcgcattttc
      121 ctcatcgatc agaatgtcaa acgagccgaa tggaccctgg tagattgacc cgtatccatc
      181 cttgaccgag cgcttggcca tggcgggaat ccccgcagcg gcaaacaggc cgacaatcat
      241 gtttgcttcc atttcgtccg aagccgtcgt gatggcgatc agttttgtat ccatgtggtt
      301 agtataccac ttcgggggcc ggctggcaaa gtgtgttgca gccaggtgag tctcgacagg
      361 actggatccc ctggtcacag atgatcacag gcacgggtca cgcagcgacc cccgcaccgg
      421 cttcatcatt gcattttgag gtcatgatcg tagaatattc acgagtaact cgtggaggct
      481 actatggtca gagtacgcta tgcaccaagc cctacagggc acattcatgt cggcaacgcc
      541 cggactgcga tcttcaacta cctgtatgct agacacgagg gtggcacgtt catcatgcga
      601 ctcgacgaca ccgatctcga acggtcgacc gaggagaaca tagactcgat gctcgacgac
      661 attcgctggc tgggactgga ttgggacgag ggcttcctca agggaggcga cctcggcccg
      721 tatcgtgaga cagagcgcaa acccctctac gacaagtacc tccagcagct gctggacgag
      781 gacaaggcat atcggtgctt ctgcaccagg gatgaggtcg aggccgaacg tcgacaagcg
      841 gaggctgaac accgcatcta ccggtacggt ggcacttgtg cgcacctgag cgccgaccag
      901 gtcgccggga acatcgctga aggcaagccg tatgtgatcc gtctgcgcat ccctgtggaa
      961 gatgtggtcg ttcacgactt gattcgaggg gacgtccagt tcaagtcgga ggaattcgac
     1021 gacttcgtca tcgcccgtca gaatggcatt cccatctaca acttcgccac cgtcatcgac
     1081 gacgcgctca tgaatatcac acacatcatc cgtgccgagg aacacttgtc caatacgccg
     1141 cgacaagtct tcatctatcg tgcactcggc ttcgatgtcc cggagttcgc tcacgtctca
     1201 ctcatccttg cgcccgatca cagcaagctc tcgaagcggc acggcgccgt gtccgtcggc
     1261 gagtaccgac ttgagggata cctgcccgag gcactgatca actacctggt gctcctgggg
     1321 tggtctccca aggacgaccg cgagttcttc accgtggacg aattgatcaa ggagttctca
     1381 cttgccggcg tcggcaagtc aggcgccgtc ttcgacaaga agaaactgac atggatgaac
     1441 cacatgtacc tgggcaggaa ggatgcggct gacactctgg cgatcctcaa gaaggacttc
     1501 gccaaggagt atgatgcagc gttcggaggg cttcccgaag acaagacgct cgccgccatc
     1561 gacctcacga aggggaatgc acaggtcctc ccggatctct tccggctcgc tcgcaacatc
     1621 gtccaggcgg ctgtctccat cacggatgag aagtcgcggg aagtcatcgg ctccaccgaa
     1681 gcgaaacagg tcttgcagta tctggtggaa cacttcgacg cgataccgtt cggtcaaggc
     1741 gatgatacga tgagtgcatg ggtggaggac ctccgcaacc agatcccact cccggccaag
     1801 aagctttaca gccctatccg cgtggcactg ttcggctcca aggacggccc ggaaatcgtc
     1861 aagctgttcc tgatgctgga gccggcatcc atccgcgagc gcctgcagaa agccctggct
     1921 ctctagacgg tcgatcactc aacaaccacg gcccctcagc gttgctgtcg ctgaggggcc
     1981 gtttcatccc gtcatcttgc cgagtcgcgg gatgcggcca gagctggagt ggaccatgag
     2041 agagatccta cagcctgttc gcttcggcga tgatcgtgtc gatgaagtca cagggcttga
     2101 cgttgcggac gggttcgcca cgaatgaaga ggacgcccag gtcgcgcccg caggcaatgc
     2161 cgacatctgc agccttccct tcgccgacgc cgttgaccac gcagcccatc aaggcaacag
     2221 tcaccggctt cacgatctcg ctcagtcgct cctcgagctg agccacgagc gacaagatgt
     2281 ccggaacctc acaacgaccg catgtcgggc acgacacgat ctgcgcgccg tgtctccgca
     2341 ggtccagtcc ggcaagcagt gtccatgccg catctacttc caggacagga tcgccgctga
     2401 tcgaatatcg aatcgtgtcg ccgatgccgt cgatgagcag
//
LOCUS       DATMZD010000293         2939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_48381,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000293 DATMZD010000000
VERSION     DATMZD010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48381"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="VN478_06120"
     CDS             complement(<1..357)
                     /locus_tag="VN478_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22283.1"
                     /translation="MTKYNELLQDIQDETSYFLPVDALSAGTHRYLYHLGDFSTANSS
                     EILRFLEDKREALGKLEQGKLSNSERRSALVLRPYMDSLSALIAAGYFADTLWQLRNS
                     RRGLYQLLYMTEIPMYI"
     gene            complement(498..1331)
                     /locus_tag="VN478_06125"
     CDS             complement(498..1331)
                     /locus_tag="VN478_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22284.1"
                     /translation="MKDPLNLSKKAVAPARPKARRTVLVVLGSLLLFALVVALIAVPV
                     LRSRNVAFYVDGKAVIEDHIAAMVTDLPFDTTSDYYKTRTDLGDPSDPKMQYLVAGLK
                     TEAVQRLIILHAQSDEAAQLGIVLDASAIDTAVEAYVKDHATADDVTEIARLHTPQMR
                     SYIQLCAVSKAYEAKLTEDTTVSSAEIRQYYATWGWNYTDAAGRQLAFEQASARLAED
                     ALANKKFQLILENRAQLLDKESSRVVGDTRYKEFMRWWDIMFGIQVPDSLQALKVDAG
                     S"
     gene            complement(1340..>2939)
                     /locus_tag="VN478_06130"
     CDS             complement(1340..>2939)
                     /locus_tag="VN478_06130"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HWQ22285.1"
                     /translation="GKESRIRQERKSGFVEKPARYHRDRFPIVMRVIAIVLIVALITW
                     GSLFAVKSATGTIAYQVGSEKVLQADITARVQNYIDMYKQYGMDLTSAQYASTLESVR
                     QNVIDSSIEQELFVQYAKSKNTKIDEAKLKTALDTEVENAVTQSKTQFGTDTKAFEDA
                     VVAQYGSLDKFKATLRTQIQPYVEQQQLADSVTAEINAGVTVSDADVKAYFASQGRVN
                     ADHLLITVDTTKDSSSTIAAKKKVAQTIYDDIVKQEAENATFDFAKYAKEKAAELNKK
                     TAGYATYEALGFFTSGQMVKEFETAAFAAKAGDIVGPVQTSFGFHIIHKIAQEPISAT
                     YDTAETVKAAHVVLGFGTGDQTAGAKAAETLANKIYAELQKGLSFKTAVTKYSTDADA
                     KTGGILDYFSAVGDATRFGAVKGLKVGEYSKPFVDGSSYEIVQVLGRKPFVKATLSDK
                     ATYDKVKTALEDERKADAKTALVAKLKEQFPVREGQWSRITKWYGRGLGKVLGAVGQW
                     IVKATGKGTTTTPATDVPTTPSAE"
BASE COUNT          536 a          843 c          847 g          713 t
ORIGIN      
        1 gatgtacatg gggatctctg tcatataaag cagctgatag aggccacgtc gggagttgcg
       61 caactgccac agagtgtccg caaagtaccc agctgcgatc agggcggaca agctgtccat
      121 gtagggccga agaacgagag ccgaacggcg ttcgctgttg ctgagcttcc cctgttcgag
      181 cttcccaagg gcctcacgct tgtcctcaag aaaccggagt atctcggaac tgttcgcagt
      241 ggagaagtct cccaggtgat acagatacct gtgcgtccct gcactgagcg cgtccacagg
      301 aaggaagtag ctggtctcat cctgaatgtc ctggagcagt tcattgtact tcgtcatcgc
      361 gggttctgcc tgaggactgt tctgtcggcc gtctgctctg gatcgtcggt tgcgaggcgg
      421 acggcttggc ttcggagcag tacttctctg actctgcggc gcgcccgcac cagcgggagt
      481 ctctcttctc ggacgtgtca cgaccctgcg tccaccttca atgcttgaag tgaatccgga
      541 acctggatgc cgaacatgat atcccaccac ctcatgaact ctttgtaccg cgtatcaccg
      601 acgacacggc tgctctcctt gtcgagcagc tgtgcacggt tctcgaggat caactggaac
      661 ttcttgttcg caagagcatc ttccgcgagt cgagcgcttg cctgttcaaa ggcaagctga
      721 cgccctgctg cgtccgtgta gttccagccc catgtggcat agtactgacg gatttcggcc
      781 gacgacaccg tggtatcttc tgtcagtttt gcctcgtagg ctttggatac agcgcagagc
      841 tggatgtagg agcgcatctg tggagtgtgc agtctcgcta tctcggtcac gtcatctgct
      901 gtggcgtggt ccttcacgta ggcttcaaca gcggtatcga tggctgaggc gtccaggacg
      961 atcccgagct gcgcagcctc gtcggactga gcgtgcagaa tgatgagccg ctgcacggct
     1021 tcggtcttga gcccggcgac gagatactgc atcttcgggt cagacgggtc gccgaggtcc
     1081 gtccgcgtct tgtagtagtc gctggtcgtg tcaaagggca ggtcggttac catcgccgcg
     1141 atgtggtctt cgatgacggc cttgccgtcg acgtagaagg cgacgttgcg tgagcgcagt
     1201 accggtacgg caatgagtgc cactacgaga gcgaacagga ggagcgaacc cagaacgacg
     1261 aggaccgtcc ggcgcgcctt cgggcgcgcc ggcgcaacgg ccttcttcga cagattgaga
     1321 gggtctttca cgggtaccgc tactcggctg atggagtggt cggcacgtcc gttgctggag
     1381 ttgtcgttgt tcccttgcct gtggccttga cgatccattg accgactgct ccgagcacct
     1441 tgccgagacc tcgaccatac cacttcgtga ttcgcgacca ctgcccctcg cggactggga
     1501 actgttcctt gagtttggcc accagcgcgg tcttcgcgtc tgccttgcgc tcgtcttcca
     1561 gcgcggtctt gaccttgtca tatgtggctt tgtcacttag tgtcgccttg acaaatggct
     1621 ttcggcccag gacctggacg atctcatagg acgatccatc gacaaatggc ttcgagtatt
     1681 caccgacctt gaggcccttg acggcaccaa atcgcgtggc gtcgcccaca gccgagaaat
     1741 agtccaggat tcctcctgtc ttggcgtcgg cgtccgtaga gtacttcgtg actgcagtct
     1801 tgaacgacag gcccttctga agttcggcgt agatcttgtt tgcaagcgtt tctgcagcct
     1861 ttgcgccggc tgtctggtct cctgtcccaa acccgagtac gacgtgcgca gccttgaccg
     1921 tctcggcagt atcgtaggtt gcacttatgg gttcctgagc gatcttgtgg atgatatgaa
     1981 agccgaaact ggtctggacg ggtccgacga tgtcgccggc cttggcggcg aaggcggcag
     2041 tctcgaactc cttgaccatt tgccccgacg tgaagaagcc gagcgcctca taggtggcat
     2101 atccagcagt cttcttgttg agctctgccg ccttctcctt cgcgtacttg gcaaaatcga
     2161 acgtggcgtt ctccgcctcc tgcttcacaa tgtcgtcgta gatggtctga gccaccttct
     2221 tctttgcggc gatcgtcgac gaagagtctt ttgtcgtatc gacggtaatg agaaggtggt
     2281 ctgcattgac acggccctgc gaggcaaagt aggccttgac gtcagcatcg gaaacggtga
     2341 cgcccgcatt gatctcggcc gtcaccgagt cggcgagctg ctgttgttca acgtacggct
     2401 ggatctgagt gcgcagcgtc gccttgaact tgtccaggga accatattga gctaccacag
     2461 catcttcaaa ggctttcgtg tccgttccga attgcgtctt ggactgggtg acagcattct
     2521 cgacctctgt atcgagagcg gtcttcaact tcgcctcatc gatcttggtg ttcttggact
     2581 tggcatactg gacaaatagc tcctgctcga tagagctgtc tatgacgttc tggcggaccg
     2641 actcgagcgt actggcatat tgcgcactcg tgaggtccat tccatactgc ttgtacatgt
     2701 cgatgtagtt ctggacgcgc gccgtgatgt cggcctgaag gaccttctca gagccgactt
     2761 gatatgctat agtcccagtg gcgctcttga ccgcgaagag cgatccccac gtgatgagag
     2821 ccacgatcaa cacgatggcg atcactcgca taacaatcgg aaaccggtcc cggtgatacc
     2881 ttgccggctt ctcgacgaat cctgatttgc gttcctgtct gatcctgctc tcttttccc
//
LOCUS       DATMZD010000294         3142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_109244,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000294 DATMZD010000000
VERSION     DATMZD010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109244"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            238..2433
                     /locus_tag="VN478_06135"
     CDS             238..2433
                     /locus_tag="VN478_06135"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWQ22286.1"
                     /translation="MKRVLCMVLAVLLMAVVLAPAATVNAAGFKDVPASLWASDEINA
                     LAAKKVIAGYADGTFKPQANVSREEFAKMIVTAKGLTLVKPARATFSDVPSSRWSYGY
                     VEAAAKAGYVSGIGGGKFGPTAAIKRQDLAVLLVRVLGKSSVASAIKSSVAFANDEDK
                     IGAYAVGAVSIAVQPRVQVLKWDEDRNINPVKSATRADCAFAIYNTVNAVTSGKKTLT
                     MAEENYPENLFPLISDSAYTMKAVTYLSSEPISMTPYGVLFPEMAKYVPTIANKHLIR
                     NADGSVITDMELRPGMKWSDGQPVTIDDYIFSHQLYMSDDIQVVSRTPMDLVTKIVKV
                     SDLKCRIYWKSWDPYIPAGWYIYPKHILGPQYTKDPKSINTSTFNNDPVYCGPYTLKT
                     KVEGQYMTLAANKNWFGGQPVIENITMKAIDNNNTLLINMLTGQIDVSSESLAIDLAQ
                     QFESKMGKSFNVYYNKSTSAGILEWNLTSKWFKDSRVRQAFYYGIDRKLITQKAMVGT
                     DAVLSPLSSGSIYYKPVLAEYAYSPDKANALLDAAGWKWNAAKTERVLPDGSPAVLKI
                     PYSQGATFRIKEVTLIQPMLAKLGITVEHDGTDFNAMLDAATAGTFIINLHGISFSSY
                     DAFGSVLAFRSDQIPTEKNGMQGQNNYRYSSADMDKWLNIAQNATTVTALKEAYSHVQ
                     DIFAKDLPCLYLEQRVYPDEVRKGLKGYDHFFSSTVYNNWNIQYWYWWK"
     gene            2556..>3142
                     /locus_tag="VN478_06140"
     CDS             2556..>3142
                     /locus_tag="VN478_06140"
                     /inference="COORDINATES: protein motif:HMM:NF040248.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWQ22287.1"
                     /translation="MRDYIIRRILGMIPLILIVMIVAFGIFSLVPNPFAALIENPRIK
                     KSDIQRLIKAWGFDLPWYLRFFKWFSGVIRGDWGPSLLYPGSTARDVISRALPVTVRV
                     MGIEFLVALAIALPIGIISAVKQYSMTDYIVTVISFIGMSMPTFWFGLMMMMLFSVVL
                     KRPSGLPLLPAGGIITPGLEEAPFWAKLGDRAQYLV"
BASE COUNT          693 a          892 c          843 g          714 t
ORIGIN      
        1 cgttcaattg acactctcag ccgaccgtgg tataacgtca cgatgggtaa gtacctggtc
       61 ggctcggtcg aggaagacgc cgggccgcta tcctactttg gtttctctga tgctggctct
      121 gcgtacagag ccacagcttg cagataaacg gtcatagtgt agggaggtgt gtcttaccga
      181 aagagcacgc gaaaaggctt ttgcatgtct tacatctatt ctaggaagga gtatgcgatg
      241 aagagagttc tatgcatggt tttggcagtc cttctcatgg ctgtggtgct tgcccccgct
      301 gcaaccgtga acgctgccgg cttcaaggac gttcctgcgt cactctgggc ttcagacgag
      361 atcaatgcgc tagctgccaa gaaggtgatc gccgggtatg ccgatggcac gttcaagcca
      421 caggcgaacg tctcccgcga ggaattcgcc aagatgatag tcacggccaa gggtctcact
      481 cttgtgaaac ccgccagggc gacattcagc gatgttccct catcccgctg gtcctacggc
      541 tatgtcgaag cagctgcgaa ggctggctat gtttctggca ttggcggtgg caagttcggt
      601 cccacagcgg caatcaagcg ccaggacctc gccgtacttc tggttcgtgt tctcggcaag
      661 tcttccgttg cgtcggcaat caagtcttct gttgcgtttg ccaacgatga agacaagatt
      721 ggcgcatacg ctgtcggagc cgtgagcatc gcggttcagc cgcgtgttca ggttctgaaa
      781 tgggacgagg accggaacat taatccggtc aagtcggcga ctcgcgcaga ttgtgcattt
      841 gccatctaca acacggtcaa tgccgtgact agcggcaaga agacactgac catggcggag
      901 gagaactacc cggagaacct gttcccgctt atctccgact ccgcgtacac catgaaggct
      961 gtcacctacc tgtcgagcga gccgatctca atgactccgt acggtgtcct gttccccgag
     1021 atggccaagt acgttccaac cattgcgaac aagcacctca ttcgcaatgc tgacggctcc
     1081 gtcatcactg acatggaact tcgacccggc atgaagtggt cagatggtca gccggtcacg
     1141 atcgacgact acatcttctc acaccagctc tacatgtccg acgatatcca ggtggtctca
     1201 cgcactccta tggatctagt caccaagatc gtgaaggtca gcgatctcaa gtgccgcatc
     1261 tactggaaga gctgggatcc ctatattcct gctggatggt atatctaccc gaaacacatc
     1321 ctgggacccc agtacaccaa ggaccccaag agcatcaaca cttctacgtt caacaatgat
     1381 cctgtgtact gtggcccgta cacgctcaag accaaggttg aaggccagta catgacactt
     1441 gccgccaaca agaactggtt tggtggtcag cctgtcatcg agaatatcac catgaaggcc
     1501 atcgacaaca acaatacgct cctgatcaac atgctgaccg gtcagatcga cgtctcctcc
     1561 gagtcacttg ccatagacct tgcccagcaa ttcgagtcca agatgggcaa gtccttcaat
     1621 gtgtactaca acaagtccac atctgcaggt atccttgaat ggaacctgac gtcgaagtgg
     1681 ttcaaggact cacgggtccg gcaggccttc tattatggta tcgaccgcaa gctgatcact
     1741 cagaaggcca tggtcggtac tgatgcagtc ctttcgcccc tctcctcggg atccatctac
     1801 tacaagcctg tccttgccga gtatgcctat agcccggaca aggccaacgc gctccttgac
     1861 gctgccggtt ggaagtggaa tgctgccaag acagagcgcg tcctgcccga tggatcgccg
     1921 gccgtcctga agattccata ttcgcagggc gcgacgttcc gtatcaagga agtcactctt
     1981 atccagccca tgctcgcgaa gctcggtatc acggtggagc atgatggaac ggacttcaac
     2041 gcgatgctgg acgcggccac ggcaggcaca ttcattatca acctgcatgg tatcagcttc
     2101 agcagctacg acgcattcgg atcggtcctc gcattcagga gcgaccagat tccgactgag
     2161 aagaacggca tgcagggcca gaacaactac cgctacagca gcgccgacat ggacaagtgg
     2221 ctcaacatcg cacagaacgc cacgacggtg actgcgctca aggaagcgta ttctcatgtt
     2281 caggacatct tcgccaagga ccttccctgc ctgtatcttg aacagcgcgt ctaccctgac
     2341 gaagtccgca agggactgaa aggctacgac cacttcttct cgtctactgt gtacaacaac
     2401 tggaacattc agtattggta ttggtggaag taggtacgat gaccaggtct gtggacgtcg
     2461 cgattgcgga gcctgaggac ctgtagatct gcttcatccg ctcccacgcc tccaaagaga
     2521 ggcgtgggag actgccgaaa ggagtcttcg ccaacatgag agattacatt atccggcgta
     2581 ttctgggcat gatcccgctc atcctgattg tgatgatcgt ggcttttggc atcttctctc
     2641 tcgtgccaaa cccgttcgcg gctctgatcg agaacccgcg aatcaagaag tccgacatcc
     2701 agcgcctgat aaaggcctgg gggttcgacc ttccctggta cctgaggttc ttcaaatggt
     2761 tcagtggcgt tatccgcggt gactggggtc cgtctctgct ctaccctggg tccacggctc
     2821 gggacgtcat ctcgagggca ctgcctgtga ctgtgcgggt catggggatc gaattcctgg
     2881 tggcccttgc tatcgctttg cccattggca tcatctccgc cgtgaagcag tactctatga
     2941 ccgactacat cgtgacggtc atcagcttca taggtatgtc catgccgacc ttctggtttg
     3001 gactgatgat gatgatgctc ttcagtgttg tcttgaagcg gccaagtggg ctgcccctgc
     3061 tgcctgctgg gggcataatc acgccgggat tggaggaggc tccgttctgg gcaaagctcg
     3121 gcgatcgtgc acagtacctg gt
//
LOCUS       DATMZD010000295         5607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_148579,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000295 DATMZD010000000
VERSION     DATMZD010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5607
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148579"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1207
                     /locus_tag="VN478_06145"
     CDS             <1..1207
                     /locus_tag="VN478_06145"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HWQ22288.1"
                     /translation="SATIADALTRCGHRSSERLDEASVVVINTCAVREKAEEKAFSFL
                     GEAAKIVGASHIVFMGCTATLDRERAVRIAGRGLKVVAGTAGLEQVLSVVGTVVPISD
                     SDCGAPDSLFPTADIELVRGCESYCTYCIVPRSRGPEIIVDAGEVLHRAERAIGSGFS
                     EILFLGQNVNRYRSSLGGLVEMMSRVDGLDGDFWFWFLSSHPANFTSSEVQRLMDLRH
                     IEHRLHLPLQSGSDDVLEHMNRRYTIADYAALVAPVRHNGDWTLTTDIIVGFPGETDD
                     DFARTVDAVRRFAFDGVFLAKYSDRPGTPSSRMTDKVAPSVIDERHSVLLKIVQDLSE
                     MSNQAVAGSTVEVLVLSANADGSVFGRSIDGRNVWFTCCEPVPGVGAFVRVMVNHGSR
                     EGLYGSRIC"
     gene            1188..2135
                     /gene="miaA"
                     /locus_tag="VN478_06150"
     CDS             1188..2135
                     /gene="miaA"
                     /locus_tag="VN478_06150"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HWQ22289.1"
                     /translation="MAVGSASLPLVVGIVGATGTGKTALAMGIARRFGGSIISADSMQ
                     VYRGMDVGTAKLSLAERACVPHALIDVVPPTHCFSVAEYQTLALEAVQAAIRRGRLPI
                     LVGGTGLYVSAVLDGYEFLEEKPDGALRSELRALPEEMLRQRLKATDPTAEREIALND
                     SRRLARALEMTIQSGELPSALKRRRHAVAWRVLRIGLRVDRSELYRRLDRRVDAMLSA
                     GMEDEVRGLLADGVNSDSTAMQGIGYKELGAWICGKMGRDEAIELWKRHTRNYAKRQE
                     TWFRRDKDVRWIDVSHQTADSTLEEAVALVDSASGGDST"
     gene            2132..3385
                     /locus_tag="VN478_06155"
     CDS             2132..3385
                     /locus_tag="VN478_06155"
                     /inference="COORDINATES: protein motif:HMM:NF018540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HWQ22290.1"
                     /translation="MIVTERELTDRAQQLEIDYATDIAGIRALQRRNFMRVLSAFHTA
                     GIATSDLRPMSGYGIGDSLREKTEHVFALLYGAQAALVRPQIISGTHALAIALFGVLR
                     PGDLLCCVTGAPYDTMEEIIGIRPSDGSLAEFGVRYTQCDLTTLPPELWEEQLAKCFS
                     TQMPRLVLIQRSRGYKRIRALREADIAHMIACVKRMSPDTLVLVDNCYGDLLEDREPT
                     MDGADLLASSFMKNVGAGIVPTGGYVVGTSRAVALAATRYSSPGVGSEIGPNLGFAET
                     FLRGLLFAPIVMEQVLVGSLLASALLQENGFVVDPGPHEPRGDIVLRIECRSRGCFTA
                     FARAVQECSALDADAVPEASPMQGYDREILMAGGTLVQGSTVELSFDGPACEPYVGYL
                     QGGLNAWQVVQATARGIAYSMNAHE"
     gene            3456..4703
                     /gene="glyA"
                     /locus_tag="VN478_06160"
     CDS             3456..4703
                     /gene="glyA"
                     /locus_tag="VN478_06160"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HWQ22291.1"
                     /translation="MTGLARVDPEVADALNNELHRQQNNIELIASENFVSEEVLAAQG
                     SVLTNKYAEGYPGRRYYGGCEYVDVVETLAIDRAKALFGAEGANVQPHSGTQANLEVY
                     FSVLQPGDTILSMSLADGGHLSHGSPVSLVGKLFHIVSYGVRKDTETIDYDQVAELAR
                     TEHPKMVVAGASAYPRIIDFPRFREIADSVGAFLLVDMAHIAGLVAAGLHPSPVPYAD
                     FVTTTTHKTLRGPRGGIILYREQYAKQLNSAVFPGGQGGPLEHVIAAKAVALKQAASP
                     EFATYQKQVLANARVLAQALMDRGYRIVSGGTDTHLMLVDVSSRGVTGRDVQALVETV
                     GITVNKNAIPFDPLPPNKASGIRLGTPATTTRGMGEAEMVEIAALIDETIVARDDAEK
                     LAQLRGRVTALTDRFPLYADKAY"
     gene            4716..5348
                     /gene="upp"
                     /locus_tag="VN478_06165"
     CDS             4716..5348
                     /gene="upp"
                     /locus_tag="VN478_06165"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013289912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HWQ22292.1"
                     /translation="MDYCNLQVLNHPLIRHKLTLLRNRNTTHKDFRQLAEELAGLMTY
                     EVCRNMRTKACLIDTPLEPYSGQELENEVTIVPVLRAGLVMAQGMLDMIPNAKVGHIG
                     LFRDENTLEPVQYYCKLPESTGESDVFVVDPMLATGGSASKAISLVKERRPHSICFCC
                     IIAAPEGIEVLQREHPDVKIVTLEIDRRLNENGYILPGLGDAGDRIYGTK"
     gene            5350..>5607
                     /locus_tag="VN478_06170"
     CDS             5350..>5607
                     /locus_tag="VN478_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22293.1"
                     /translation="MHLVITQAQLWSFLVALALSLVLTPVVMRLGLRLGITDKPAPSA
                     EAHVDAQRRRVNRKVTPRTGGIAIAAAFGVSVFLFNGLTLQL"
BASE COUNT         1158 a         1543 c         1744 g         1162 t
ORIGIN      
        1 cagcgcgaca atcgccgatg cgctgacacg ttgtggacac aggtcttcgg agcgtcttga
       61 tgaagcatct gttgtcgtga tcaacacctg cgccgtccgt gagaaggcgg aggagaaggc
      121 gttcagtttt ctcggtgagg ccgcaaagat agttggagcc agtcacatcg tgttcatggg
      181 ttgtaccgcc acacttgaca gagaacgggc ggttcggatc gccggtcgag ggctgaaggt
      241 cgtagcaggc accgccgggc tggaacaggt tctctcggtc gtggggaccg tggtgccgat
      301 cagtgactcc gactgcgggg ctcctgattc gttgttcccg acagcggaca ttgaacttgt
      361 gcgcggctgt gagagctact gcacgtactg tatcgtcccg agatcgcgag gtcccgagat
      421 aatcgtcgac gccggcgagg ttctccatcg ggccgagcgg gccatcggca gtggtttctc
      481 tgagatcctc ttcctgggac agaacgtcaa caggtaccgg tccagccttg gagggcttgt
      541 ggagatgatg tcgcgagtcg acgggctgga cggagatttc tggttctggt tcctgagttc
      601 ccatcctgcc aacttcacgt cgagcgaagt gcagcgactc atggacttgc gccatatcga
      661 gcaccgattg cacctgcctt tgcagtcggg aagtgacgat gtcctcgaac acatgaaccg
      721 gcggtacacg atcgcggact acgcagcgct cgtggcacct gtccgccaca acggcgactg
      781 gacgttgaca acagacatca tcgtgggctt cccgggagag acagatgatg actttgcccg
      841 caccgtcgat gcggtccgcc gattcgcgtt tgacggggtc tttcttgcca agtactcaga
      901 cagaccgggt acgccctctt ccaggatgac tgacaaggtc gcaccatcgg tcatcgacga
      961 aagacactcg gtcttgctga agattgtgca ggacctgagc gaaatgagca atcaggcagt
     1021 tgcaggcagt acggtcgaag tgctggtcct gtctgcgaat gctgacggga gtgtcttcgg
     1081 aagatcgatc gacggcagaa acgtctggtt cacatgttgc gagcctgtgc cgggagtcgg
     1141 tgctttcgtc agggtgatgg tcaatcatgg ctcgagagag gggttgtatg gcagtcggat
     1201 ctgctagcct tcctctggtg gtaggcatcg tcggtgccac tggaacggga aagaccgctc
     1261 tcgccatggg aatcgcccgt cgtttcggag gctccatcat ctccgccgac tccatgcagg
     1321 tatatcgagg aatggatgtc ggaaccgcga aactaagcct cgcagaacgt gcatgtgtcc
     1381 cacatgctct catcgatgtt gtccccccta cacactgttt ctcggttgcc gaataccaga
     1441 ctctggcgct ggaagcggtc caggcagcca ttcggcgggg acgactgccc atccttgtgg
     1501 gaggcaccgg gctgtatgtg agcgccgttc tggatggata cgagttcctg gaggagaagc
     1561 ccgacggagc gttgcggtcc gaattgcgag ctctgcccga agaaatgctc cgtcagcgct
     1621 tgaaagcaac tgatcccacc gcggaacgtg agattgctct caacgattcc aggcggcttg
     1681 cgcgggcact ggagatgact atccagtcag gggagctgcc aagcgctctg aagcggagac
     1741 gtcacgctgt cgcatggcga gtccttcgaa ttggcctgcg tgtggatcgg tccgagctct
     1801 acagacgact tgacaggcgt gtcgacgcca tgctgtctgc ggggatggaa gacgaagtgc
     1861 ggggcctgct tgcggacggc gtgaactcag acagcaccgc gatgcagggc atcggctaca
     1921 aggagttagg cgcgtggata tgcgggaaga tgggacgtga cgaggcgatc gagctctgga
     1981 agaggcacac acgaaactac gccaaacggc aggagacctg gttcagaaga gacaaggacg
     2041 ttcggtggat cgacgtgtcc catcagacgg cggactcgac tcttgaagag gcggtcgctc
     2101 tggttgactc tgcgtcggga ggagacagca catgatagtc acggagcgtg agttgacgga
     2161 ccgcgcacag cagctggaga ttgactatgc aaccgatatc gccggcatcc gcgctctcca
     2221 gcgccgcaac ttcatgcgcg tcctgtctgc gttccacact gccgggatag ccacgtcgga
     2281 ccttcgccct atgtcgggct atggtatcgg cgacagcctg cgggagaaga ccgaacatgt
     2341 cttcgccctc ttgtacggcg cacaggctgc actggtccgt ccacagatca tatccggcac
     2401 ccacgcgctt gccatcgcgc tgtttggtgt gctccgccct ggagatctgc tgtgctgtgt
     2461 gacgggtgca ccctacgaca caatggagga gatcatcggg attcgtccat ccgacggctc
     2521 gcttgcggag tttggtgtca ggtacactca gtgtgacctt acgacgctcc ctccagaact
     2581 ctgggaggag cagcttgcga agtgcttctc cacgcagatg cccaggctcg tcctgatcca
     2641 gcggagccgt gggtacaagc gcatccgggc acttcgcgag gccgatatcg cacatatgat
     2701 cgcgtgtgtg aaacgcatgt cgcctgatac cctggttctt gtcgacaact gctacgggga
     2761 tcttctggag gatcgagagc ccaccatgga cggggcagac ctgctggctt cgtcattcat
     2821 gaagaacgtc ggggcgggca ttgtcccaac tgggggctac gttgtaggga cctccagggc
     2881 ggtcgcactg gccgctactc ggtactcgag tcccggggtt ggctccgaga tcggaccgaa
     2941 tctcgggttt gccgagacgt ttctgcgggg tcttctcttc gccccgatag tgatggagca
     3001 ggtacttgtc gggagtcttc tggcaagtgc gcttctgcaa gagaacggct tcgtcgtgga
     3061 tcccggacct catgaacccc gcggggacat cgtgttgcgc atcgagtgca gatcgagggg
     3121 ttgcttcact gcatttgccc gtgccgtaca ggaatgttcg gcgcttgacg cggacgctgt
     3181 ccctgaggcc agtccgatgc agggatatga cagagaaatc ctgatggcgg ggggaacgct
     3241 cgtgcagggt tccacagtgg aactgagttt tgacggaccc gcttgcgaac cgtacgtggg
     3301 gtatctgcag ggaggtctaa acgcatggca ggtggtgcag gccacggccc ggggcatcgc
     3361 ttacagtatg aatgcccacg agtagtgtcg tcataggcca ttcgtggtag aatatccgaa
     3421 cgacgaaatg ccgaacgaat cgctgggagg ctgctatgac aggtttggca cgcgttgatc
     3481 cagaggtagc ggacgcactg aacaatgaac tgcatcgtca acagaacaac atcgagctca
     3541 ttgcatctga gaacttcgtg tcggaagaag tcctggcagc gcagggttca gtcctgacaa
     3601 acaagtatgc agagggatac ccgggaagac gctactacgg agggtgcgag tatgtcgatg
     3661 tcgtcgagac gctggcgatc gatcgcgcca aggcgctctt tggtgcagag ggagcgaatg
     3721 tccagcctca ctcggggact caagccaacc ttgaagtgta cttctcggtt ctgcagcccg
     3781 gagacacgat tctgagcatg tcccttgccg atggtgggca tctgagtcat ggaagccccg
     3841 tctctctggt aggcaagttg tttcacattg tctcctacgg cgttcgtaag gacaccgaaa
     3901 caattgacta cgatcaggtg gccgaactgg ctcgaacaga gcaccccaag atggtcgtag
     3961 ctggcgcgag cgcgtacccg aggatcatcg acttcccacg atttcgtgag attgccgatt
     4021 ctgtaggtgc gtttttgttg gttgacatgg cgcacatcgc agggctggtc gcggcaggtc
     4081 tccacccttc acccgtgcca tatgctgact tcgtcacgac gacgacgcac aagactctgc
     4141 gtggccctcg aggaggcatc atcctctaca gggagcagta cgccaagcaa ctcaacagcg
     4201 ctgtgtttcc gggcggacag ggaggacctc tcgaacatgt tatcgccgcg aaggcggtcg
     4261 ccctgaaaca agctgcgtct cctgagtttg ccacttatca gaagcaggtt ctcgcaaatg
     4321 cgagagtcct ggcacaagcg ctgatggatc gtggctatcg catcgtctca ggcgggaccg
     4381 atacacacct catgctggtc gacgtgagtt ccagaggggt caccggcagg gatgttcagg
     4441 cccttgtcga gacggtcggc atcacagtca acaagaacgc tattcccttc gatcctttgc
     4501 ccccgaacaa ggcgagtggc atacgccttg ggacgccggc aacaacaact cgtggtatgg
     4561 gcgaggcaga gatggtcgag atcgctgccc tgatcgacga gacgatcgtt gcacgcgacg
     4621 atgctgagaa gctggcacag ctcagaggcc gcgtgactgc actcacagat cgttttccac
     4681 tatatgctga caaagcctac taggagaaag aactgatgga ctactgcaac cttcaggtgc
     4741 tcaaccaccc gttgatacgg cacaaactga cgcttctgcg aaaccggaat acaacgcata
     4801 aggacttccg gcaactcgcc gaggaactgg ccgggctcat gacgtatgag gtctgcagaa
     4861 acatgcggac caaagcgtgt ctcatcgaca ctcccctgga gccctatagc gggcaggaac
     4921 ttgagaatga ggtcaccatc gtgcctgtgc ttcgagccgg gctggtcatg gcccagggaa
     4981 tgctggacat gatccctaac gccaaggtag gacacattgg tttgttcagg gacgagaaca
     5041 cgttggaacc cgttcagtat tactgcaagc ttcccgagag cactggtgag tcggatgtct
     5101 tcgtcgtaga tccgatgctt gccactgggg gcagtgcctc caaggcgatt tctctcgtca
     5161 aggaacggcg tccacacagc atatgcttct gctgtatcat tgcggctcct gaaggaattg
     5221 aggtcctgca gcgcgagcat cctgacgtca agatcgtcac gctggaaatt gatcgccgcc
     5281 tgaacgaaaa cgggtatatc ttgccgggcc ttggcgacgc cggcgacagg atatacggga
     5341 cgaagtagca tgcacctcgt aatcacacaa gcgcagctct ggtcgtttct cgtggccctg
     5401 gcgctcagcc ttgtcttgac gccagtggtc atgagactcg gcttgcgcct gggtattaca
     5461 gataagcctg ctccaagtgc ggaggctcat gtcgacgccc agcggcgaag agtgaaccgg
     5521 aaggtgactc ctcgtaccgg cggcatcgcg atcgcagccg catttggcgt gtctgtattc
     5581 ctgttcaacg ggctcacact gcagctg
//
LOCUS       DATMZD010000296         3510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_28745,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000296 DATMZD010000000
VERSION     DATMZD010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28745"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="VN478_06175"
     CDS             complement(<1..639)
                     /locus_tag="VN478_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22294.1"
                     /translation="MKRFRRPKKRVVLLVIAVLVLAVSVYLVAQKLKSNGTPGTTTNP
                     SKTDVGLYLDKGDLWQWVGGRKTQQTKVGTIVAFDWHPAGGEIVYVTSEKDGSYKTVD
                     RKLASRQELVIGQGKAEMGPKILCSSSAVLLVEASATTTPRLLQVTVTSVGYDGTANG
                     SFWTQVPLTKEQFDRTTRLILVASKPLIVSPGGIWTIGTSSLGFDTAHTFLGP"
     gene            complement(654..2705)
                     /locus_tag="VN478_06180"
     CDS             complement(654..2705)
                     /locus_tag="VN478_06180"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HWQ22295.1"
                     /translation="MRYVIKRTGEKVSFDQAKIRIAITKAGKATKELDEAEPERLTRI
                     IVAQIPDDIATVEQIQDLVEQTLMESRHHRTAKAYILYREQHRKLRDLKTLLDPRETI
                     QKYLEKLDWRVNENSNTSYSLQGLNMHLATSVVSRYWLNEVYPPEVRDAHVSGDMYVH
                     DLGYLAAYCVGWDLKDLLVRGLGGVYGKVESKPAKHFRSALGQMVNFIFTMQGEAAGA
                     QAFSSVDTYLAPFIRYDHLRYDEVKQAVQEFVFNMNVPTRSGFQSPFSNITLDLRPHG
                     ATATEAAVIGGKPVVETYADFQPEMDMFNRAFAEVMLAGDSKGRVFSFPIPTYNITKD
                     FDWSNPVYDPIFEMTAKFGIPYWSNFVNSDMDPDDTRSMCCRLRLDTRELRKRGGGLF
                     ASNPLTGSIGVVTIDLPRIGYLSHSRDELFERLSHVMDVARTSLEIKRKIIENLTAAG
                     LYPYSRHYLDAVKQRNGAYWANHFNTIGINGMNELLLNFSGVTIGEPDGRRLALEIMD
                     FMLGKLEEFQHESGQLYNLEATPAESATYVLAKQDKALYPDILVANEEAVRGGADPYY
                     TNSTQLPVTYTTDPFEALDLQDPLQVKYTGGTVVHLFLGERLDSADQAKQLVRAVTNS
                     YRLPYFTLTPTFSICPRHGYIAGNHPFCPRCDEELIAEAQKTSVNPISADVPFAVRPE
                     S"
     gene            complement(2898..>3510)
                     /locus_tag="VN478_06185"
     CDS             complement(2898..>3510)
                     /locus_tag="VN478_06185"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HWQ22296.1"
                     /translation="VKLVEQLGEIANLARARQPSATSQAVTEAIRNIPSWLQVAGAIF
                     FSLVFVAMAVIVAVILSKAAFDTRFGFLLNITDPRERKRVAASIRRMSSLDESLLTAS
                     PREAVIALFYMGVAALQDLDLSLARGETPEELVTRVRERSGTVASFLDLLVTTFYVAR
                     YSDQDIRPQQALACRDAYTSLVGAVKTENEGKRASRTHLVTIK"
BASE COUNT          670 a          974 c         1119 g          747 t
ORIGIN      
        1 tggaccgagg aatgtgtgag ccgtgtcgaa gccaaggctt gaggtgccaa tcgtccagat
       61 gcctccggga gaaacgatga gaggcttcga tgcgacaagg atcaatcggg tcgtacggtc
      121 aaactgttcc ttggtcagcg ggacctgggt ccagaaggaa ccgttcgcag tgccatcgta
      181 gcccacagag gtgacggtga cctggagcag gcgcggagtg gtagtcgcgg atgcctcgac
      241 cagaaggacg gcactcgaag aacacagtat cttgggtccc atctcagcct tgccctggcc
      301 gatgaccagt tcctgcctgg atgccagctt gcggtcgaca gtcttgtacg aaccatcctt
      361 ctcgctggtc acgtaaacga tctcgccacc ggcgggatgc cagtcgaacg cgacgatcgt
      421 gccgaccttg gtctgctgtg tctttcgtcc acccacccac tgccacaggt cccccttgtc
      481 cagatacagg ccgacatcgg tctttgaggg attcgtggtc gtccccggtg tgccgttcga
      541 cttcagcttc tgtgcaacaa ggtagacgga cacagcaaga acgaggacgg cgatgacaag
      601 gaggacaact ctcttcttcg gacgacggaa tctcttcatg gggcagacac ctcctagctc
      661 tcgggacgga cagcaaatgg gacgtctgcg gagatcgggt tcacagacgt tttctgcgcc
      721 tccgcgatca gttcctcatc gcatcggggg cagaacggat gattgccggc gatgtacccg
      781 tgcctggggc aaatggagaa cgtgggcgtc aacgtgaagt agggcagccg atagctgttc
      841 gtgaccgcgc gtacgagctg tttggcctgg tcagcgctgt ccagccgttc gccgaggaac
      901 aggtggacga ctgtgccgcc cgtgtacttg acctggagag ggtcctgaag gtccagggct
      961 tcaaacgggt cagtggtgta cgtcacgggc agctgtgtag agttggtgta gtacggatcg
     1021 gcgccacccc tgacggcttc ttcatttgca acgaggatgt cggggtacag ggccttgtcc
     1081 tgcttggcga gcacgtaggt tgctgactcc gcgggtgtcg cttcaaggtt gtagagctgc
     1141 ccgctttcat gctggaactc ctcgagtttc cccagcatga aatccatgat ctcaagtgcc
     1201 agtcgtcttc cgtcgggttc gccgatcgtg acgccgctga agttgagcag gagttcgttc
     1261 atcccgttga tgccgatcgt gttgaaatga ttcgcccagt aggccccatt gcgttgcttc
     1321 accgcatcca ggtagtgacg cgaatatggg tagaggcctg ccgcagtcag attctcgatg
     1381 atcttgcgct tgatctcgag cgacgtgcga gcaacgtcca taacgtgcga caggcgctcg
     1441 aacagctcgt ccctgctgtg ggagaggtag ccgatgcgtg gaaggtcgat agtgaccacc
     1501 ccgatggagc cggtgagggg gttggaggca aacagtccgc cgcctctctt ccgcagttcg
     1561 cgcgtatcca gtcgcagacg gcagcacatc gatcgggtat cgtccggatc catgtcgctg
     1621 ttcacgaagt tgctccagta ggggatgccg aacttggcgg tcatctcgaa gatcgggtca
     1681 tagactggat tgctccagtc gaaatccttc gtgatgttgt atgttgggat cgggaacgag
     1741 aacacgcgcc ctttggagtc gcctgccagc atgacctcgg cgaaggcacg attgaacatg
     1801 tccatctccg gctggaagtc cgcgtaggtc tcgaccacag gctttccgcc tatgacggct
     1861 gcctcggtgg cagtggcgcc atgcgggcgc agatcgagag tgatattgct gaacggggac
     1921 tggaaacccg accgggtcgg gacgttcatg ttgaacacaa attcctgcac ggcctgcttg
     1981 acctcgtcat accgcaggtg gtcgtagcgg atgaagggtg cgaggtaggt gtcgacggaa
     2041 ctgaaggcct gtgcccccgc tgcctcgccc tgcatggtga agatgaagtt gaccatctgc
     2101 cccagcgcag agcggaaatg cttggcgggc ttgctctcga ccttgccgta cacgcccccg
     2161 agtcctctca caaggaggtc cttgaggtcc cagccgacac agtaggcagc caggtagcca
     2221 agatcgtgta catacatgtc gccgctgacg tgcgcgtccc gcacctccgg cggatagacc
     2281 tcgttcagcc aataccggct gacgacgctg gtcgcaaggt gcatgttgag gccctggagg
     2341 gagtagctgg tgttcgagtt ctcgttgacg cgccagtcca gtttctccag gtacttctgg
     2401 atagtctcgc gcgggtccag cagggtcttg aggtcgcgca gcttccggtg ttgctcacga
     2461 tagagaatgt aggccttggc cgtcctgtga tgacgcgact ccatgagcgt ctgttcgaca
     2521 aggtcctgga tctgctcgac tgtggcgatg tcgtcgggta tctgagcgac gataatgcgc
     2581 gtcaagcgtt ccggttcagc ctcgtcgagt tccttcgttg ccttgccggc ttttgtgatg
     2641 gcgattcgga tcttggcctg atcgaaggat actttctcac ctgtgcgctt gatgacgtat
     2701 cgcatgcgta tcgcccccaa ctacgattga tggtcttgac tcattctgcg caggatactt
     2761 ctgactgcca cgcccttgtc aagcatgtga ccattctagg gcgacgcgct agtgcgtcaa
     2821 gcattgtgaa gatgcgggtc aggggcttga cagaaccgaa atacctgttc caggaagggg
     2881 gtctcgcacc gttccgtcta cttgattgtg acgaggtgcg tgcgggacgc ccgcttgccc
     2941 tcattctctg tcttgacagc cccgacaagg ctcgtgtagg cgtccctgca tgcgagagcc
     3001 tgctggggcc ggatgtcctg atccgaatag cgggcaacgt agaacgtcgt cacgaggagg
     3061 tccagaaagg acgcaacggt tccggaccgt tcacggaccc gtgtcaccag ttcttcggga
     3121 gtctctccgc gggctagaga gaggtccagg tcctgaaggg cggccacacc catatagaag
     3181 agggcgatga cggcttcacg cgggctcgcc gtcagaagcg actcatccag tgacgacatg
     3241 cgtcggatcg acgcggcgac gcgttttcgt tcacgagggt ccgtgatgtt gagcaggaaa
     3301 ccgaagcgag tgtcgaatgc agccttcgag aggatgacgg cgacgatcac ggccatggcg
     3361 acgaagacga gggagaagaa gatggcgccg gcgacctgca accaggaagg gatgttgcgg
     3421 attgcctcgg tgactgcctg gctggtggcg gagggctgcc tggcgcgggc gaggttggcg
     3481 atctcgccga gttgctcaac cagcttgacc
//
LOCUS       DATMZD010000297         3591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_48412,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000297 DATMZD010000000
VERSION     DATMZD010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48412"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VN478_06190"
     CDS             complement(<1..334)
                     /locus_tag="VN478_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili twitching motility protein PilT"
                     /protein_id="HWQ22297.1"
                     /translation="MDEFNKAPEEIRGLDDILKVVVDRGASDLHLQAGTPPMLRLHKH
                     LLPIGTEMMSGSGIEALVYPIMNDDQKAVFKEHLDFDFSYSVKGLARFRVNVFRQRGT
                     MAVTMRRLS"
     gene            589..1563
                     /locus_tag="VN478_06195"
     CDS             589..1563
                     /locus_tag="VN478_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22298.1"
                     /translation="MNTNTRIWENSIAGLPKGDQQAERFEHYAREFFMPDSIGPSVYL
                     ANVGALMEQADTESGVQPSPSAARMALSTQHPTGFPTWFLAAYPDVALWFASRDGLDR
                     QAVDAELTRRSATSGATASHLVDENGFFAHVVLRTLIDMYRERTGSVLECTAPEADLQ
                     EEGATGSTDERALTAQQAVDLMAANITRYERELATSYGERFAGMQPRLVAAGLLQLGY
                     YVLVDHVIPLTTLVHAADESSSADDPVVEFVERLEIEVFCAEGGETERKRIEEACHLV
                     RSRILDVVLQTRTDFEQTPSLAPATRNFMENPELEPLRLAVGIRPSSL"
     gene            complement(1589..1954)
                     /locus_tag="VN478_06200"
     CDS             complement(1589..1954)
                     /locus_tag="VN478_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22299.1"
                     /translation="MFLLVAVLNSDLHVRKVLEGFTAIDIGGATVIDSRGMGQMLAAD
                     IPIVAGIARLLGGSPVQESSRTIFSVISNMKTLERAKKIVLSAVGDMTQKGVGIMFVV
                     PVAEAYGLIEPEPLPPSIP"
     gene            complement(2083..>3591)
                     /locus_tag="VN478_06205"
     CDS             complement(2083..>3591)
                     /locus_tag="VN478_06205"
                     /inference="COORDINATES: protein motif:HMM:NF024487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HWQ22300.1"
                     /translation="KLALSDELMKRSFDVVIIDEASMSLVPYDVLAASRAHGKIVFAG
                     DFMQLPPIASGTTAAVREWLQRDIYQVRGITKAVRADQDVPDMAMLRTQYRMHPSIRS
                     AVSRLFYQDRLDDGERIIVRTSSIARLAPFEGHALAAVDTSLLQTRCYREVKGYSRLN
                     LVEAVTVMTIASGMLSRYPDISIGIVTPYSVQARLLGTMARELTLDRERLTISTVHRY
                     QGSEVDVVIFSLTDGEPQWSMSTLTRGNLEDWDGDPAPRLLNVALSRARGKFILVGDL
                     DFVHDRGAQDAVLRHIVGIIEHEGAVRRVDPRALSELREWLPNRVVDGQRIVVGERAR
                     HQGWLLEQARAARHTVTAGVPQGAHVSPLMARLLASVSPDCRLHYGDHDGLRTAAEKF
                     GASESYAGEGVLESFLQIGSHPLLLEGTLEHGARSAYLALDLPATNWFFLKNAGFVPE
                     PLLGRNPEAVKGKGRRRRGLGDDMTAQDIASASSPHRSVAPAQSEETALPLD"
BASE COUNT          695 a         1148 c         1071 g          677 t
ORIGIN      
        1 gagacaggcg ccgcatggtc acggccatcg ttcccctctg ccggaacacg ttcacgcgga
       61 accgtgccag gcccttgacg gagtacgaga aatcgaagtc caggtgctcc ttgaacactg
      121 ctttctggtc atcgttcatg atgggataca cgagcgcttc gatgccgctt cccgacatca
      181 tctcggtccc gatgggcagc agatgcttgt ggagacgaag cataggcggc gtacctgcct
      241 gcaggtgcag gtctgatgcg ccacgatcga caacaacctt cagaatatca tccaggcccc
      301 tgatctcctc gggcgccttg ttgaactcgt ccatcagtac ctccgagtcg gccgtgcaat
      361 agcagatgta cgtactgcac tgacagacag tatagcagca cattggtttc ttctactcat
      421 gaaacgcatg tcccttcccc atcatggtcc ccggcgatca tccgctgccg ggccgatcgt
      481 gcatacccct tcgacaggac gcaattgaca aatcagcctt gaggcggtag catgaattga
      541 tagtcatgga gcacatgacc accgtgctca accgctggga ggcctgtcat gaataccaac
      601 actcgcatct gggaaaacag cattgcaggc ttgccaaagg gagaccagca ggctgagcga
      661 ttcgagcact acgcccgcga gttcttcatg ccagacagta ttggtccttc tgtctacctg
      721 gccaacgtgg gcgcgctgat ggaacaggcg gacacggaga gtggagtcca gcccagtccg
      781 tctgcggcca gaatggccct gtcgacccag caccccaccg gctttccaac gtggtttctc
      841 gcagcctatc cggacgtcgc cctctggttc gcgtcccggg acggcctgga ccgtcaggct
      901 gtcgacgccg agttgaccag aaggtcagcg acatccgggg ccacagccag tcatctcgtg
      961 gacgaaaacg ggttcttcgc ccatgtcgtc ctgcggacgc tcatcgacat gtaccgcgaa
     1021 cgcactggct ctgtgctgga gtgcacagct cccgaagccg accttcagga ggaaggggcg
     1081 accggttcga ccgatgaacg ggcactcacc gcccagcagg ctgtcgatct gatggccgcc
     1141 aatatcaccc gctatgagcg tgaactcgcg acgagctacg gagagagatt tgccgggatg
     1201 cagccgcgcc tggtcgctgc ggggcttctt cagctcggct attatgtcct tgtggaccac
     1261 gtcatcccgc tgactaccct ggtccatgct gccgacgaat cgtcgtctgc cgacgatccg
     1321 gtcgtcgaat tcgtagagag gcttgagatc gaggtgttct gtgctgaggg tggcgaaacc
     1381 gagcgaaagc ggatcgaaga ggcgtgtcat ctcgtgcggt cgcgcatcct ggacgttgtc
     1441 ctgcagaccc gcacggactt cgaacagacc ccgtcgcttg ccccggccac ccgcaatttc
     1501 atggagaatc cagaactcga gcccctgcgt ctggctgtcg ggatccgtcc ctccagcctg
     1561 tagaggtttg ggcccgaagc gaatccagct aggggatgct gggcggcagc ggttccggct
     1621 cgatcagtcc gtatgcctct gcgaccggca caacgaacat gatgccgact cccttctggg
     1681 tcatgtcccc gacggcactg agcacgatct tcttggccct ctccagcgtc ttcatgttgc
     1741 tgatgaccga gaagatcgtc ctgctgcttt cctggacggg agaaccaccc agcagacgcg
     1801 cgatgccggc tacgatgggg atgtcggccg caagcatctg acccatgcct cggctgtcga
     1861 tgaccgttgc gccaccgata tctatggcag tgaagccctc caggactttg cgtacatgta
     1921 gatcactgtt cagaacagca accagcagga acatcgtcgc ctccgcagaa cacgcgtctt
     1981 cgtcactagt ttggcggatg actggccact gtcaactgcc acaccctgag gaagacctgc
     2041 aagacactat tcataggttt ctgacagccg cggccccgca aactagtcca gtggaagagc
     2101 tgtctcctcg gactgggccg gcgctacact gcgatgcgga ctcgatgcgg atgcaatgtc
     2161 ctgcgcggtc atatcgtcgc cgagcccgcg gcgtctgcgc cccttaccct tcacagcctc
     2221 agggttgcgt ccaagcaggg gctcaggcac gaaccccgca ttcttgagga agaaccagtt
     2281 ggtcgcaggc aagtcgagcg ccaggtacgc cgaccgcgcc ccgtgttcca gcgtaccctc
     2341 cagcagcagc ggatgagaac cgatctgcag gaagctttcg aggacgccct ctcctgcata
     2401 cgattcggat gcgccgaact tctctgcagc agtgcgcagg ccatcatggt cgccgtagtg
     2461 caggcggcag tcgggcgaca ctgacgccag caggcgtgcc atgagcggcg agacgtgcgc
     2521 tccctgcggc acgcccgcgg tcaccgtatg gcgcgcagca cgtgcttgtt ccagcagcca
     2581 gccctgatga cgggcgcgtt ctccaacgac gatgcgctgg ccgtccacca cgcggttggg
     2641 cagccactcc ctgagttcgc tgagagccct cgggtcgacg cgccgaacag ctccctcgtg
     2701 ctcgatgatg ccgacgatat gtctcaggac tgcatcctgg gcgccgcggt cgtgcacgaa
     2761 gtcgaggtcg ccgaccagga tgaacttgcc gcgcgcacgg ctcagcgcga cgttgagcag
     2821 gcggggcgcc ggatccccgt cccaatcctc gagattgccg cgtgtcagcg tcgacatgga
     2881 ccactgcggc tctccgtcgg tcaacgagaa gatgacaacg tccacctcgg acccctggta
     2941 tcggtggacc gtcgagatcg tcagccgctc ccgatccaat gtgagttcgc gggccatcgt
     3001 ccccagcaga cgggcctgca cggaataagg ggtgacaata ccgatgctga tgtcaggata
     3061 gcgggagagc atgccggacg cgatcgtcat caccgtgacc gcctccacca ggttgaggcg
     3121 cgagtatcct ttgacctcac ggtagcagcg tgtctgcagc agcgaggtat ccacagctgc
     3181 cagcgcgtgc ccttcgaagg gagccaggcg cgcgatcgag ctggtacgca cgatgatgcg
     3241 ttcgccgtcg tcgagccggt cctggtagaa cagcctgctc actgctgaac ggatagacgg
     3301 gtgcatgcga tactgtgttc gcagcatggc catgtccggc acgtcctgat ctgcgcggac
     3361 cgccttcgtg atgccgcgca cctggtagat gtcgcgctgg agccactcgc gcacggcggc
     3421 cgtcgtgccc gaggcaatcg gaggcagctg catgaaatcg cctgcaaaga cgatcttgcc
     3481 atgcgcacgc gatgccgcca gcacgtcata tggcaccagg gacatgctcg cctcgtcgat
     3541 gatgacgacg tcgaacgagc gtttcatcag ctcgtccgac agagccagct t
//
LOCUS       DATMZD010000298         3303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_28777,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000298 DATMZD010000000
VERSION     DATMZD010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28777"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1117
                     /locus_tag="VN478_06210"
     CDS             <1..1117
                     /locus_tag="VN478_06210"
                     /inference="COORDINATES: protein motif:HMM:NF019292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HWQ22301.1"
                     /translation="DRLDRILLHRVFGWLTLVAAVFTMFGLIFVLGGRLSSALDVLFG
                     FFQKAFLVLPMVPWLKDFLWGGVLQGILAGVSVALPYIVPFYIILSFLENSGYLARMA
                     FLTDAIMHRMGLHGKAFIPIILGFGCNVPAVLGTRIMERDRDRFIAGVLATLVPCSAR
                     TVVILGLVGAFMGFWPALSLYAVSLLIVFVVGRALNRLIPGTNPGLIMEMPNLKWPPL
                     RITFRQTWFRLKDFVVFAFPIIAAGSIVLYIVDALGWLTPLTTWAAPFTQGVLGLPPV
                     ALVVLIFGILRKELALIMLATLMHTPHLATVMTPQQMYVFALIVMLYVPCVSTIAALR
                     REFGNRRAALVSAGEVVLALAIGALANWGWNIVSLLR"
     gene            1201..2823
                     /locus_tag="VN478_06215"
     CDS             1201..2823
                     /locus_tag="VN478_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22302.1"
                     /translation="MTQEILSERLNLYEAMFERNPRDGLVSVGKTYLYILSDRPQDAL
                     KSALEASLSVSSKSLRVQLAIALGYYLRNKPGEALIENRYALNIDPAAPLCHLVHASI
                     LLALRRNDEARLAFLKCLSLSSDNTFLTDLRLRNRRAICAAQLQNPLLAAYSNLRLRI
                     PDTTEADAQQLVEQNPSDPVLKVLLASLERRSGKIREALFLLENSLASYEAFPERLYL
                     LWKTYDDQLGNRQKGEAFVRRLLEVDPLNDRATGLGYSNLDEETLRHINFIKSLEDLP
                     DSIFARILPIRELEELIGTSDTDALDTTAPTPCDEKSATVLERSFTLPGTMQEPLVPK
                     LEARLQSSPEVAPPAAEPSSASQPVMAHMADMLEQAQLIARDLRPTPAPTPMSPHGVA
                     THAPVPEPAAEQLAPGQPASDAVLQVPEPAAESVAAVSERPVESVADKAVTAPPEEVV
                     NSVLEPLSATLAPEPAEEAFDPASVVAQSAPPAAPEPATTVEDAPTFTPSEQQLQADG
                     PVRVTMRYANGLLGESRFEEALQAFLELSRRS"
     gene            2898..>3303
                     /locus_tag="VN478_06220"
     CDS             2898..>3303
                     /locus_tag="VN478_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22303.1"
                     /translation="MRKLRLISCLVVVMVCCSLLAPVVDAATTFAADLDLYKTNIKTT
                     SDAVRDLTAYVAAERLFARSLKRPTAQLRVLVRSYLTVLDQVDSSTRRIVRDQRASDR
                     LLADVLALAHGKRVEAAWIVLKRGAAGQERFLA"
BASE COUNT          635 a         1077 c          917 g          674 t
ORIGIN      
        1 tgaccgcctc gacaggatcc tgctccaccg cgtattcggg tggctgacgc tggtcgccgc
       61 cgtattcacg atgttcggcc tgatcttcgt tctcgggggc cggctctcct cagccctgga
      121 cgtgctcttc ggcttcttcc agaaggcatt cctggtcctc cccatggtac catggctgaa
      181 ggacttcctg tggggaggag tcctgcaagg catcctagca ggggtctcag tcgctctgcc
      241 gtacatcgtc cccttctaca tcatcctgtc gttcctcgag aactccggat acctggcacg
      301 catggcattc ctgactgacg ccatcatgca ccgcatgggt ctgcacggca aggcgttcat
      361 tcccatcatc ctggggttcg ggtgcaacgt ccccgctgtc cttgggaccc gcatcatgga
      421 acgcgaccgt gaccgcttca tcgccggggt gcttgcgacg ctagtaccgt gctcagcgcg
      481 caccgtcgtg atcctgggac tcgtgggagc cttcatgggt ttctggccgg cactctcgct
      541 gtatgcagtc agcctcctca tcgtcttcgt cgtcgggcgg gccctgaacc ggctcatccc
      601 tggaacaaac cccggactca tcatggagat gcccaatctg aagtggccgc ccctgcgcat
      661 cacgttcaga caaacctggt tcagactcaa ggacttcgtg gtcttcgcat ttcccatcat
      721 cgcagctggc tcgatcgtcc tgtacatcgt cgacgcgctg ggatggctga ctcccctcac
      781 gacatgggcg gcgcctttca ctcaaggggt cctcggactt cctcccgtgg cactcgtcgt
      841 cctcattttc ggcatcctgc ggaaggaact tgccctcatc atgctggcaa cgctgatgca
      901 tactccccac ctggcgaccg tcatgacgcc tcagcagatg tatgtcttcg ctctcatcgt
      961 catgctctac gttccctgcg tcagtaccat tgcagcgctg agacgcgagt tcggcaaccg
     1021 tcgagcggca ctggtgagtg caggcgaggt cgtcctggcg ctggccatcg gcgcactggc
     1081 gaactggggc tggaacatcg tctccctcct tcgctaggtg agaacaaggg tgtgcagccg
     1141 caccacaatg ctttgacaaa acggctctca cggcaataat gaccgaggag aggtgacgca
     1201 atgacacagg aaatcctgag cgaacggttg aatctgtacg aggctatgtt cgagcgcaac
     1261 ccgcgcgacg ggctggtcag tgtaggaaag acgtatctgt acatcctctc cgatcggccg
     1321 caggatgctc tgaagtctgc gctggaggcc tcgctgtcgg tctcctcgaa gagcctccgc
     1381 gttcagctgg ccatcgcgct cgggtattac cttcgcaaca aacccggtga agcactcatc
     1441 gagaaccgct acgcgctcaa catcgatcca gccgcccccc tctgtcatct cgtccatgca
     1501 agcatcctgc tggctctgcg acgtaacgac gaggccagac tcgcttttct gaagtgcctc
     1561 tcgctctcat ctgacaacac gttcctcact gatctcagac tccgcaatcg cagggcgatt
     1621 tgcgctgcgc agttgcagaa tccactgctc gccgcgtaca gcaacctgag actgcggatc
     1681 cccgacacga ccgaggccga cgcacagcaa ctggtcgaac agaacccaag cgaccccgtg
     1741 ctgaaagtac ttctggcttc cctcgaacga cgcagcggca agatccggga ggccctcttc
     1801 ctgctggaga atagcctggc aagttatgaa gcatttccag agcgactcta cctgctatgg
     1861 aagacgtatg atgaccaact cggcaaccgt cagaaagggg aagctttcgt tcgccgcctg
     1921 cttgaggtcg acccgctcaa cgatcgggcc actggtcttg gctactccaa tctcgacgaa
     1981 gagaccctgc gccacatcaa tttcatcaag tctctcgaag atcttcccga ttccatcttc
     2041 gcaaggatcc ttcccatcag ggaactggaa gagttgattg gaacctcgga cactgacgcg
     2101 ctcgatacaa cggcccccac accttgtgac gagaagagcg ctaccgtgct ggaaaggtcg
     2161 tttaccctgc cgggcaccat gcaggagcct ctggtcccca aactggaggc acgtctgcag
     2221 tcctctcccg aggtcgcgcc tcccgcggcc gagccttcct cggcatccca acccgtcatg
     2281 gcccacatgg cggacatgct ggaacaggcc cagctcatcg cccgcgatct gcgaccgacg
     2341 cccgcaccga ccccgatgtc gccgcacggc gtggcaacgc atgcacccgt gccggaaccg
     2401 gccgcggagc aattggcccc cggacagccc gcctccgatg ccgtgctgca ggtgccggaa
     2461 ccggccgcag aatctgttgc tgctgtctct gagagacccg tcgagtccgt cgcagacaag
     2521 gctgtcaccg caccacctga agaagtggtc aactcggtcc ttgaacccct gagtgccact
     2581 ctcgctcccg agccggctga ggaggcattc gacccggctt cggtcgtcgc tcagtcggct
     2641 cccccagccg caccggaacc ggcaactacc gtcgaggacg cacccacatt caccccttcc
     2701 gaacagcagc tgcaggcgga cggaccggtc cgagtgacaa tgagatacgc caacggcctg
     2761 ctgggggagt cgcgattcga ggaggcactc caggcattcc tggaactcag tcgcagaagc
     2821 tagcaccaat cggaacaggg ttgtgcccat ctggtgaaca ttgcaggaac attggtggtc
     2881 taatcacagg tgatgtgatg agaaaactga gactgatttc ctgtcttgtc gtggttatgg
     2941 tctgttgctc actgttggct ccagtcgttg atgcagcgac gacatttgct gcagacctgg
     3001 atctgtacaa gaccaatatc aagaccacgt cggacgctgt acgcgatctc acggcgtatg
     3061 ttgccgccga gcggctgttt gcacggtctc tcaagaggcc aacagcacag ttgcgcgtgc
     3121 ttgtccgttc gtacctgacc gtcctcgacc aggtggatag ctcgacccga cgcatcgtgc
     3181 gagaccagcg tgccagtgat cgcttgctgg ccgacgtcct tgcactggcc cacggcaagc
     3241 gcgtggaagc agcttggata gtcctgaaga gaggcgcagc aggtcaggaa cggtttctgg
     3301 caa
//
LOCUS       DATMZD010000299         2786 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_89179,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000299 DATMZD010000000
VERSION     DATMZD010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89179"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="VN478_06225"
     CDS             <1..524
                     /locus_tag="VN478_06225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWQ22304.1"
                     /translation="RALKILQDEGEQWTVLFTITGHENACARQLAGEAKGLGGRVILA
                     GQLPREQIAEFCTQSILVFPSWLETFGMPLMEAREAGGWVVAADTPSTRETLDGYSRA
                     ILSERASPVDLARCMRLAWNARHDSAGQIQAEDVQPAGSSAGGGWEGVASIVGSTIRA
                     AWLRREALRGGSL"
     gene            524..1564
                     /locus_tag="VN478_06230"
     CDS             524..1564
                     /locus_tag="VN478_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007292165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HWQ22305.1"
                     /translation="MKASCILVTGGTGSFGHSFLTYALARGVQEVRVLSRDEFKQEIM
                     RKELHDPRVKFYLGDVRDRASVDHAMEGVDHVFHAAALKEVPSCEFFPVQAVMTNVLG
                     SSNVIESALAHGVKSVVCLSTDKAVQPVNAMGMSKALMEKTAYAAARDGGHTRHTVIA
                     VTRYGNVMCSRGSVIPLFIEKMKHGEPLTVTEPTMTRFLMSMDEAIGLVLFAMEHAAP
                     GDLFIRKAPACTIGDLALALKQLFHADVPIQVIGSRHGEKLHETLATAAELHRAEDMG
                     DYFRVSMDDRDLNYEKYVTEGDQGLLMTGDYTSASTQRLSVDEIIAKLAALPEVQREL
                     NGGSGSSFARGS"
     gene            1566..2687
                     /locus_tag="VN478_06235"
     CDS             1566..2687
                     /locus_tag="VN478_06235"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWQ22306.1"
                     /translation="MRIGITGADGFLGWHLRCLLHGDTHDTAMPCDQASFADDTALND
                     LVAASDAIVHFAGINRGTDDEVLRGNVAITDRLIAALSRSGRSPHVLFASSVQIERDN
                     PYGQAKRVCGQHLQAWADGCGARFTTVVLPNVFGEFGRPFYNSVVATFCHQLVHGEGP
                     HAAVDAPLELIHAQEVVARFVMLAHEATVGTVRVQGHAPLLVSEVLEHLRHQNAMYAG
                     GTFPLLADPFDLQLFDTLRSFCFPDQAQGTFDVHSDVRGSLWEIARAQGAGQTFVSWT
                     RPGITRGNHYHRHKVERFAVLSGSGRIRLRRLFTPDVYTYDLTGEAPGYVDMPTFCTH
                     SIENTGSTPLLTLFWSGELFDPASPDTWAEPVLVQEATA"
BASE COUNT          490 a          909 c          865 g          522 t
ORIGIN      
        1 agcgtgcgct gaagatcctc caggacgagg gggaacaatg gacagttctg ttcacgatca
       61 cgggtcatga gaatgcatgc gcgcggcagc tcgcgggcga ggcgaagggc cttggtggaa
      121 gagtcattct tgctgggcag cttccaaggg aacagatcgc agagttctgc acccagtcga
      181 tcctggtgtt cccgtcgtgg ctggagacat tcggaatgcc cctcatggaa gctcgggagg
      241 caggaggttg ggtggtcgct gcagacacac cgtccaccag ggagaccctc gacggctatt
      301 ccagagctat actcagtgag cgcgcctccc ccgtggatct cgccagatgc atgcgcttgg
      361 cgtggaatgc tcgtcacgac agtgcggggc agatccaggc tgaagatgtc cagcctgctg
      421 ggtcgagtgc tggtggagga tgggaaggcg tggcatcgat cgtcggttcc accatacgtg
      481 ccgcctggct gaggagagag gcattgcgag ggggctctct gtgatgaaag catcgtgtat
      541 tcttgtcact ggtggtactg gctcgtttgg tcactccttc ctcacgtatg cccttgcccg
      601 gggagtgcag gaggtccggg tcctgagccg tgacgagttc aagcaggaga tcatgcgcaa
      661 ggagctgcac gacccacggg tcaagttcta cctgggggac gtgcgggacc gtgcgagtgt
      721 cgaccatgcc atggagggcg tcgaccacgt cttccatgcc gcagccctca aggaagtccc
      781 gtcctgtgag ttcttccccg tccaggccgt catgaccaac gtgctgggca gcagcaacgt
      841 catcgagtcc gctctcgccc acggcgtgaa gagcgtcgtc tgcctcagca ccgacaaggc
      901 ggtccagccg gtcaacgcca tgggcatgag caaggctctc atggagaaga ctgcatatgc
      961 tgcagcgagg gacggagggc acacgagaca cactgtcatc gccgtcaccc ggtacggcaa
     1021 cgtcatgtgc tcccgtggct ccgtcatccc cctgttcatc gagaagatga aacacggcga
     1081 acccctgact gtcaccgaac ccaccatgac ccgcttcctc atgtccatgg acgaagccat
     1141 cggcctcgtg ctgttcgcca tggagcacgc ggcgccgggc gacctgttca tccgcaaggc
     1201 tcccgcctgc accatcggtg accttgccct tgccctcaag cagctcttcc atgcggacgt
     1261 ccccatccag gtcatcggtt cgcgtcacgg ggagaaactg cacgagacac tcgcgacagc
     1321 tgccgaattg caccgggcgg aggacatggg cgactacttc cgcgtgagca tggacgaccg
     1381 tgacctcaac tacgagaagt acgtgaccga gggcgaccag ggcctcctca tgactggtga
     1441 ctacacctcg gcctccaccc agcgcctgtc ggtggatgag atcatcgcca agctggcggc
     1501 gctccccgag gtccagcggg aactgaacgg cgggtccggc tcgtcgtttg cccgggggag
     1561 ctaagatgcg catcgggatc accggggcgg acggtttcct cggctggcac ctgcggtgcc
     1621 tgctgcacgg agacacgcat gacactgcca tgccctgtga ccaggcatcg ttcgccgacg
     1681 acacagcatt gaacgatctc gtggccgcct ccgatgccat cgtccacttt gccggcatca
     1741 accgtggcac ggacgacgag gtcctgcgcg gtaacgtcgc tatcaccgat cggctcatcg
     1801 cggccctgag caggtccggt cggtcccccc acgtcctgtt cgcctcctcc gtccagatcg
     1861 agcgcgacaa cccctacgga caggccaagc gtgtgtgtgg ccagcacctc caggcatggg
     1921 cggacggctg cggggcacgc ttcaccacag tcgtcttgcc caacgtattc ggtgagttcg
     1981 gccgtccctt ctacaattct gtggtggcga ccttctgtca ccagctcgtg cacggcgagg
     2041 ggccccatgc tgctgtcgat gctcccctcg agctcatcca cgcccaggaa gtggtggctc
     2101 gcttcgtgat gcttgcccac gaggcgacgg tgggcacggt ccgtgtgcag ggtcatgctc
     2161 ccctgctggt ctccgaggtt ctggagcatc tccggcatca gaacgcgatg tatgcaggtg
     2221 gcaccttccc gcttctcgcc gacccgttcg acctgcagct gttcgacacc ctgcgctcct
     2281 tttgctttcc cgaccaggca cagggtacct tcgacgtcca ctcagacgtg cgcggttcgc
     2341 tgtgggagat tgcacgggcg cagggagcag gccagacgtt cgtgtcctgg acccggccgg
     2401 gcatcacgag gggcaaccac taccaccggc acaaggtgga gcgcttcgcc gtcctgagcg
     2461 gcagcgggcg catccgcttg cggcgcctgt tcaccccgga tgtctacacc tatgacctga
     2521 cgggtgaagc gccgggctac gtggacatgc cgaccttctg cacccacagc atcgagaata
     2581 ccgggtccac tccactcctg accctcttct ggtccggtga gctgttcgac ccggcgagtc
     2641 cagacacgtg ggccgagccc gtcctggtcc aggaggcgac cgcatgaagg tcatgaccat
     2701 cgtcgggacc cgtcccgaga tcatccgtct ctcccgcgtc attccttgcc tggatgctca
     2761 cgtccagcac gtcctggtcc acaccg
//
LOCUS       DATMZD010000300         5108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_129003,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000300 DATMZD010000000
VERSION     DATMZD010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5108
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129003"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /locus_tag="VN478_06240"
     CDS             complement(<1..896)
                     /locus_tag="VN478_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22307.1"
                     /translation="MRQQKMTLSERADLLARHAVAHARQVDLIATVDRDPEHASPDIL
                     QELRHWGEEIDSLRGRYMADLPVVALSRSPWSGDVLEYAMDVSGLDGLWWDYGQPLRP
                     LQQLPADFIGLAGALLPGAPTEKTPFEVRPGPEVPYVVPRLLALSGVCAVLSQIQIGP
                     HTGYAVAYFADHPEQVSNRVNTWGTDFYEVIDGQGVLTWSFDGDVAALDRDFDLKPWM
                     EAGKLLWIQPGDASMTLRPDVQACPFLALTGSHMDSTFFDGIRTEDEPDALAQEATEA
                     ALGQPVSADPVPDELWNLVMPT"
     gene            complement(906..3734)
                     /locus_tag="VN478_06245"
     CDS             complement(906..3734)
                     /locus_tag="VN478_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22308.1"
                     /translation="MEFNEATQSYIALRQQLASGAITPEHFQQEVAKLRLVTTDGVWW
                     QIDPASGAWLAWNGKTWIPAAAAGQVTQQDLQAEKSPKTSPRRGGRSRRPQPKEEPAP
                     QTLWQLAVLMVRTWLVNLPATIISSIVTGGLVWLFHTVLLVGPNASVSWTNRHPILYW
                     MIDTVQNPRGGVAFWVVASYFVTSFFGRLFSSPVACVKGIVLFPSWLTRGFRKLRLRA
                     FVPFLLGGIVAFAISWRVRNYMTVYAYALGFLLVTMALDMSFEHMTLRLALSDLRRYL
                     RVRVAPYGTESDFVFLLFPGLALGFAASALRRTPTLWAQLIFWGALLALVLSAAWSLR
                     RKATRTAGALLLFLAVVCTVVVALTPVLADDGGWPESGRTIQGLVRNAGWPIVVKLGI
                     PPAEAAILAGLLATNLGAAFGYMRSHGIDPTKLGLPDTPPPPGMKVAPDSVALTPEQR
                     RARAERAVADAVKAQAEAAEANSWGGLLSAAWKNWEGEAVQIGDAVSGLVAGTKDLVV
                     KGATSLYDGARAVYNDPSIVTAPLKNLANDIANAARDAWNDPQIIWDTASGTWKDIKT
                     GASVAVDVGSKIVSGVASSIYTTLTDPQKMWEAIKDSGGWDNWVKSWDPNVPVLDRFG
                     NVLIGTLKIGATILTAGQVKAAALAGREILSVAGAQILRGDFKAGARSLINGVIKTFV
                     SGETKAAQAIADDAARLKGGVLKPVVSGVPSTGVNPGHLKTIQEGAQKFGVEVGIRPQ
                     GPISGFVRNGVPKGSAIKNKGTSFIDGLIGSKAPEGAVGHFRPDQKAVDRLIEGIKRN
                     PGIPGARKAEMIREIEGRVADRIGELAGPKVGQLIKEGKLTVKGGALIDTASGQPVIS
                     DLDLWSLTKTGGGPVTAAQEKAFVEWCAARGVPVTHGAHMNWVPRTPADYKVYEKIIR
                     SHGLGGKPIITVGSGGATGAANYIPPAP"
     gene            complement(3836..4180)
                     /locus_tag="VN478_06250"
     CDS             complement(3836..4180)
                     /locus_tag="VN478_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22309.1"
                     /translation="MRYSRKAVIVVAVLVLLLITMTGCRHRSSLAGPVTSRDDAVARV
                     TALPDVAAWIKLVQTESPANRVVIEVDSEDAALYTVHVYELVQDDAGGHSATFGWYEV
                     VKATGIVRLVMP"
     gene            complement(4177..>5108)
                     /locus_tag="VN478_06255"
     CDS             complement(4177..>5108)
                     /locus_tag="VN478_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HWQ22310.1"
                     /translation="PVEGIHVLRPPGDVFALFLDGTTLWCGGVDGVCAVDTRTRMVTT
                     VVAAGRPMTYVRGIARDGDGSLWFASERGLVRWKDGQATWFTTKEGLPDNRVNCVLVT
                     REGDLWAGTWRGAALRTAHGWTVLGKQDGLADDMVRVLLEDSWGSLWFGSYVAPAGGL
                     AIRNASGWQTFSTAAGLPHNDVVSVLEAPDGTVWVGTGFLDDGGAVQFERQGESWQPR
                     RTLGKAGGLAGGKARSIYVTGGGSLIVGSEYQGMAVETPEGFRLLTTADGLSSDEVKS
                     MVEDPDGNLWCATRDGLTVIDRKTLLKIEGGAS"
BASE COUNT          951 a         1760 c         1424 g          973 t
ORIGIN      
        1 tctgtaggca tcacaagatt ccagagttca tcagggacag ggtcggcgga gaccggttgt
       61 cccagagcgg cttcggtcgc ctcctgcgcc agagcatccg gctcatcctc cgtgcgtatt
      121 ccatcaaaga acgtgctgtc catatggctg cccgtcagcg caaggaacgg gcaggcctgc
      181 acatctgggc gaagagtcat cgacgcatcg ccgggctgta tccacagcag ctttccggcc
      241 tccatccacg gcttcaggtc aaagtcgcga tccagtgcgg caacgtcacc gtcgaaggac
      301 cacgtcaaca cgccctggcc gtcgatgact tcgtagaaat ccgtacccca cgtgttgacg
      361 cgattggaca cctgctcagg gtggtcggca aagtaggcaa ccgcgtaccc tgtgtgcggg
      421 ccgatctgga tttgactgag tactgcgcag acgcccgaca acgccaacaa gcgcggcacg
      481 acgtagggga cctcgggtcc gggccggacc tcgaacggcg tcttctccgt cggagccccg
      541 gggagcagcg cccctgcaag gccgatgaag tccgcaggca gctgctgcaa cggccgcaat
      601 ggctggccgt agtcccacca aaggccgtcc agtccgctga cgtccatggc gtactccagg
      661 acatcgccgc tccaggggct cctggacagt gccacgacag gaagatcggc catgtaccgg
      721 ccccgcaggc tatcgatctc ctctccccag tgtctcaatt cctgcaggat gtctggcgac
      781 gcatgctcgg gatcccggtc tacggtcgcg atcaggtcga cctgccgggc gtgtgccacg
      841 gcatgccgcg caagcaggtc agctcgctct gacagcgtca tcttctgttg tcgcatggca
      901 gcctcctatg gagccggcgg gatgtagttg gcggcgcctg tggcgccgcc tgatccgacc
      961 gtgatgatcg gctttcctcc cagcccgtga cttcggatga tcttctcgta aaccttgtag
     1021 tccgccggcg tccgaggtac ccagttcatg tgcgctccgt gtgtgaccgg gactcctcga
     1081 gctgcgcacc actcgacgaa cgctttctcc tgggcggccg ttacgggccc tcctcccgtc
     1141 ttcgtcagac tccacaagtc caggtccgag atgaccggct ggcctgaggc agtgtctatg
     1201 agggcccctc ccttcaccgt cagcttccct tccttgatga gctgccctac tttcggtccc
     1261 gcaagctcgc cgatcctgtc tgcgacccgg ccctctatct ctctgatcat ctcagccttt
     1321 cgcgcacccg ggatccctgg gtttctcttg atgccctcga tgagtctgtc gaccgccttc
     1381 tggtcgggtc tgaagtgtcc gactgctccc tcgggtgcct tgctgccgat cagtccgtcg
     1441 atgaagctag tacccttgtt cttgatggct gaacccttgg gcactccgtt cctcacaaat
     1501 ccggagatag ggccttgcgg gcgtatgcca acctcgacac cgaacttctg tgccccttcc
     1561 tggatcgtct tcaggtggcc ggggttgacc ccagtcgacg ggactccact gacgactggc
     1621 ttcagcactc cacccttcag tctggcagca tcatctgcaa tggcttgcgc ggccttcgtc
     1681 tcccccgaca cgaacgtctt gatgacgcca ttgatgaggg accgcgcgcc cgccttgaag
     1741 tctcccctga gaatctgagc accggcgacg ctcaggatct ctcttcctgc gagagccgcc
     1801 gctttcacct ggccagccgt gagaatagtc gccccgatct tcagagtccc gatcaggacg
     1861 ttcccaaacc gatcgaggac ggggacattg gggtcccacg acttcaccca gttgtcccag
     1921 ccacccgagt ccttgatggc ttcccacatc ttctgcggat ccgtcagagt cgtgtagatg
     1981 ctgctggcga cgcccgagac gatcttgctc ccgacatcca cagccacgga tgcgccagtc
     2041 ttgatatctt tccacgtgcc gctggccgta tcccagatga tctgagggtc attccacgcg
     2101 tctcgggcgg cattcgcaat atcgttcgca agattcttca ggggtgcagt gacgatgctt
     2161 gggtcgttgt agaccgctct cgcaccgtca tagagagacg tagctccctt cacgaccaga
     2221 tccttcgtcc ctgcaacaag gcccgacaca gcgtccccga tctgtacagc ttcaccttcc
     2281 cagttcttcc acgctgcaga cagcagccct ccccacgaat ttgcctcggc tgcctccgcc
     2341 tgtgccttga cagcatctgc gacggctctt tccgctctcg ccctcctctg ttcaggcgtc
     2401 agagcgacac tgtctggtgc taccttcatg ccgggaggag gaggggtgtc cggcagtccg
     2461 agcttcgttg ggtcgatgcc atggctcctc atatacccga acgcggcccc aaggttcgtc
     2521 gccagcagtc ctgccaggat cgccgcctcg gcgggcggaa tccctagctt cactacaatt
     2581 ggccacccgg cgttcctgac gagaccctga atcgtacgtc cactctcggg ccatccgccg
     2641 tcatcagcaa gaacaggtgt cagtgcgacc acgacagtgc atacgacagc aaggaacagc
     2701 agcaatgcac ccgcagtcct ggtcgctttg cgtcgcagag accacgctgc cgaaagcacc
     2761 agggcgagaa gcgctcccca gaagatgagc tgcgcccaga gtgtcggtgt ccgcctcagg
     2821 gcggaggcgg caaaccccag cgccaagcca gggaacagca gaaacacgaa gtctgactcc
     2881 gtaccatacg gagcgacccg cacccgaaga taccgccgca ggtccgacag cgccaaccgc
     2941 agtgtcatgt gctcaaaact catgtccagc gccatcgtca cgagcaggaa cccgagcgca
     3001 tatgcataga ctgtcatgta gttgcgcact ctccagctga tggcaaatgc gacaatgccc
     3061 cccagaagga acggaacaaa ggcccgcagt ctcagtttgc ggaacccgcg cgtcagccag
     3121 gacggaaaca gcacgattcc tttcacgcaa gcgacgggtg acgaaaagag cctgccaaag
     3181 aacgaggtca cgaagtagga ggcaaccacc cagaaggcga cgccgccccg cgggttctgc
     3241 accgtgtcga tcatccagta gaggatggga tgcctgttgg tccacgagac gctggcgttg
     3301 gggccaacca gcaacactgt gtgaaacagc cataccaggc cccccgtcac aatactggag
     3361 atgatcgtcg cgggcaggtt cacaagccaa gtcctcacca tcaggaccgc gagctgccac
     3421 agcgtctgcg gcgcaggttc ctccttgggc tgtggccggc gacttcgtcc tccacgcctg
     3481 ggagacgtct tcgggctctt ctctgcttgc aggtcttgtt gcgtcacctg gccggctgca
     3541 gccgccggaa tccatgtctt gccattccag gcgagccatg ccccacttgc aggatcgatc
     3601 tgccaccaga cgccgtctgt cgtcaccagg cgcagcttcg ccacctcttg ctggaagtgc
     3661 tccggcgtga tagcaccgct tgcgagctgt tgccgcaacg ctatgtacga ttgagtcgcc
     3721 tcgttgaact ccatgaatgc ctcccgttca ccctgacttg cgcctgtcaa cggatacatg
     3781 gccaatccca actgctgtct caagtatgca gaatccgatc cgaccgtgca agagcctacg
     3841 gcatgaccaa gcggacgata cctgtggcct tgacgacctc ataccagccg aacgttgccg
     3901 agtgaccgcc cgcgtcgtcc tgcacaagct catagacatg cactgtatag agagctgcgt
     3961 cctccgaatc gacttcgatg acgacccggt tagccgggct ttcggtctgc accagcttga
     4021 tccatgccgc cacgtcggga agagctgtca cacgcgcaac tgcatcgtcc cgcgacgtga
     4081 cggggccggc gagcgaggac cggtggcggc acccggtcat ggtgatgagc aatagaacaa
     4141 ggactgcgac cacaatgacc gccttccgcg agtacctcat gacgctcctc cttcgatttt
     4201 caacagtgtc ttgcggtcga tgacggtgag gccatcccgt gtcgcacacc agaggttgcc
     4261 gtcaggatct tccaccatgg acttcacctc gtcgctggag agtccatctg ccgtcgtgag
     4321 gagcctgaac ccctcaggtg tctcgacagc catgccctgg tactcggatc ctacgatcag
     4381 gctgccaccc cctgtcacgt agatcgaacg ggccttccca ccggcgaggc ctccagcctt
     4441 gcccagcgtc cgccgtggct gccagctctc gccctggcgc tcgaactgga ccgctcctcc
     4501 gtcgtcgagg aaccccgtcc cgacccacac cgtgccgtca ggcgcctcca gaaccgagac
     4561 cacatcgttg tgtggcaggc cggccgccgt ggaaaatgtc tgccagccgg acgcattgcg
     4621 tatcgccagg cctccggcgg gtgcaacata ggagccaaac cacaggctgc cccagctatc
     4681 ctcgagcagg acacgcacca tgtcgtctgc gagaccgtcc tgcttgccca ggaccgtcca
     4741 cccgtgggcc gtgcgcagtg ccgcaccccg ccacgtccct gcccacaggt cgccttcccg
     4801 cgtcaccagg acacaattga cgcggttgtc gggcaatccc tccttcgtcg tgaaccacgt
     4861 cgcctgtccg tccttccagc gcaccagtcc acgctcggac gcaaaccaga gcgaaccatc
     4921 cccgtccctg gcgatacccc gcacgtaggt catcggacga ccggctgcta cgaccgtagt
     4981 caccatgcgg gtccgggtgt cgacagcgca tacgccgtcc acaccaccgc accagagcgt
     5041 cgtaccatcg aggaacagcg cgaagacgtc tcccggcggc cgcaggacgt gaatgccttc
     5101 gacaggct
//
LOCUS       DATMZD010000301         2848 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_59219,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000301 DATMZD010000000
VERSION     DATMZD010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59219"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1123
                     /locus_tag="VN478_06260"
     CDS             <1..1123
                     /locus_tag="VN478_06260"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22311.1"
                     /translation="ESCLTQPVRFDHDTSLRGPRLGPRSPVHYRCLRYRRKRMSCYTA
                     FMKRPDNASPGETAAPKTGKSTGAGVSVDLTTVERVQAMVLGWYAAHARRFVWRVPNP
                     DPYVVLVSETMLQQTQTARIQEKLPLFLRQYPDFRALAATDNASIVRAWQGMGYNNRA
                     LRLRDCARAVVERFDGVLPDTVDDLRSLPGLGPYTVSAILSFAFHRDVVVLDVNIRRL
                     YSRLFLQLTTTLDIMPEPLLLYVAGEVYPRGRGSDWHQACMDIAAQYCTARAPRCLFC
                     PVAKACRSAFRLAEAVPPRVAEPSHRDKPNRIWRGRIIQLLRGLAPGETMGLTELRTQ
                     LFPIEEETDGPWLERILAALERDGLVDCDHTSNVIRLRS"
     gene            complement(1232..2044)
                     /gene="proC"
                     /locus_tag="VN478_06265"
     CDS             complement(1232..2044)
                     /gene="proC"
                     /locus_tag="VN478_06265"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HWQ22312.1"
                     /translation="MSDRTRISFIGGGVMANAFVNSLLDRGVTSGDDIMVSSRSWATL
                     EVMRERHHVHTTLSNREAAQFGNVVLLAVKPQNVDRVFADIKGVMRPEQVLLSIVAGV
                     DIDRLSQGAGHDRVVRCMPNTPAQIGAGVSVWTGSPQVTQDDRTLVRSVLGAVGLEIE
                     VDEERYVDMATAVSGTGPAYVFLFIEAFMDAAVHLGFQRDMALELVLQTMEGSLKLAR
                     SSQKHVADLKNSVTSPGGTTAEALYELEKGGFRTALSKAVWAAYQRTLTLKD"
     gene            2244..2585
                     /locus_tag="VN478_06270"
     CDS             2244..2585
                     /locus_tag="VN478_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22313.1"
                     /translation="MEQVRKILIILSTAEREKAMAGLMYAKNTHENHWFDDVRVVLFG
                     PVERLLSEDKEIGDLAADIFKVMARPTACKAFADDKVVSGALKSLGCEIDQVGGIISE
                     CIRNGYVPLVF"
     gene            complement(2666..>2848)
                     /locus_tag="VN478_06275"
     CDS             complement(2666..>2848)
                     /locus_tag="VN478_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009646173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWQ22314.1"
                     /translation="LHHIAIRVDDIDAALAELKARGVQLIDETPRNGAAGARIAFVSP
                     KSTHGVLLELCERKEK"
BASE COUNT          534 a          920 c          809 g          585 t
ORIGIN      
        1 ggaatcctgc ctgactcagc ctgtccgatt cgaccatgat acctccctgc gcggcccgcg
       61 acttggtccg cgctctccgg tccattatcg ctgtctcagg tatcgccgca agcgcatgtc
      121 ctgctatact gcattcatga aacgcccgga caacgcttcg cctggtgaga ctgctgctcc
      181 aaagaccgga aagagcacgg gcgccggcgt gtccgtcgat ctgaccaccg tggagcgggt
      241 gcaggcaatg gtcctgggct ggtatgcggc tcacgctcgt cgttttgtgt ggagagtccc
      301 caaccccgac ccctacgtcg tgcttgtttc ggagacgatg ctccagcaga cgcagacagc
      361 ccgcatccag gagaagctgc ccctctttct tcggcagtat cccgacttcc gcgcgcttgc
      421 cgccaccgac aacgcatcga tcgtccgggc gtggcagggc atgggataca acaacagggc
      481 tttgcggctg cgagactgcg cgcgtgctgt cgtcgagcgc tttgacgggg tcctgccgga
      541 cacggtcgac gaccttcgct ctctccccgg acttggcccc tacacggtgt ccgctatcct
      601 ctcatttgcg ttccaccggg acgtcgtcgt tctggacgtc aacatccgga gactgtacag
      661 tcgactcttt ctccagctca cgacaacact tgacatcatg cccgaaccgc tactgctgta
      721 cgtcgctggt gaggtgtacc cccgcgggcg cggctcagac tggcaccagg cctgcatgga
      781 catcgccgcg cagtactgca cggcacgcgc cccccgatgc ctgttctgtc cagtcgccaa
      841 ggcctgccga tcagcgttca ggctggccga ggctgtccct ccccgcgtcg cggaaccctc
      901 tcacagggac aagcccaacc gtatctggcg cggccgcatc atacaactcc tgcgcggact
      961 cgcgccgggc gaaacgatgg gattgactga actcagaacc caattgttcc caatagaaga
     1021 ggaaaccgac ggaccatggc tggaacgcat cctcgccgca ctcgagcgtg acggacttgt
     1081 cgactgtgac cacacgtcca acgtcatcag gctgcgttcc tgactctcct cggccagccg
     1141 ccctgcagct tcgcacaaag gagcagctac acaatgttgg caacccccag gaggaagccc
     1201 tggactgacc ctgcaggctg ctctgtcgga ctcagtcctt gagggtaagg gtgcgctggt
     1261 aggctgccca gacggccttt gacagggcgg tcctgaaccc acccttctcc agctcgtaga
     1321 gtgcttcggc agtcgtccct cctggcgatg tgacggagtt cttgaggtcg gcaacgtgct
     1381 tctgactcga tcgtgcgagc ttcagagagc cttccatcgt ctggaggacc agctccaacg
     1441 ccatgtcgcg ctggaaccca aggtgcacgg ccgcatccat gaacgcttcg atgaacagaa
     1501 agacgtacgc aggccccgtg ccgctcaccg ccgttgccat gtcgacgtag cgctcttcgt
     1561 cgacttcgat ctcgagaccc actgcgccaa gaaccgagcg aaccagtgtc cggtcatcct
     1621 gcgtcacctg ggggctgccc gtccagacgc tcacgcctgc ccctatctgg gcgggcgtgt
     1681 tgggcatgca tcgtacgaca cggtcgtgac cggcgccctg gctcagccgg tcgatgtcga
     1741 ctccagcaac gatagacaga aggacctgtt ccggtcgcat gacccccttg atgtcggcga
     1801 aaacgcggtc gacgttctgt ggcttgaccg ccaggagaac gacattgccg aactgggctg
     1861 cctcacggtt agaaagcgtc gtatgaacgt ggtgtcgctc gcgcatgacc tcaagcgtcg
     1921 cccagctgcg tgaactgacc atgatgtcgt ctccggatgt gacacctctg tccaggagcg
     1981 aattcacgaa cgcattcgcc atgacgcccc cgcctataaa cgagatcctc gtcctgtcac
     2041 tcatgcggca acctccttga cttatttcaa agcatagccc gtttcgcggt gcgataccac
     2101 aggcgtgtga acgaatacgc atcatgcctg tcccaaagag ccattgacgc aaaacgccgc
     2161 gctgctatat gtatggtagt agttgggtac tcaatggatg tttgcgtccc ggcgcatgct
     2221 cgccggggaa caggaggcaa ctaatggaac aagtacgcaa gatcttgatc atattatcta
     2281 cagctgaacg cgagaaggcc atggccggct tgatgtacgc caagaataca catgagaacc
     2341 actggttcga cgatgtgcgc gttgttcttt ttggccctgt cgagcgactc ctctccgagg
     2401 acaaggagat tggggacctt gccgccgaca tcttcaaggt catggctcgt cccacggcct
     2461 gcaaggcatt cgcggatgac aaggtcgtga gcggcgctct caagagcctg ggttgtgaga
     2521 tcgaccaggt gggaggtatt atctcggagt gcatccgtaa cggctacgtt cccctggtgt
     2581 tctaggcatc tctctctcgc gatagtctca tgaacagcaa gcgccccggt ctcccggggc
     2641 gcttctctct tgctggtctt cttcgctact tctctttcct ctcacacagc tcaaggagaa
     2701 cgccatgcgt cgacttgggg ctgacgaagg ctatccgcgc tccggccgca ccgtttcggg
     2761 gtgtctcgtc gatcagctgg acgcccctgg ctttcaactc cgcgagggcc gcgtcgatgt
     2821 cgtcgacccg gatcgcaatg tgatgcag
//
LOCUS       DATMZD010000302         3741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_28938,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000302 DATMZD010000000
VERSION     DATMZD010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3741
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28938"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1142
                     /locus_tag="VN478_06280"
     CDS             <1..1142
                     /locus_tag="VN478_06280"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWQ22315.1"
                     /translation="AWVTGRTPQGIPGVWWHNGQEVGHEPVGPLVANLDELPIPDFGL
                     FDRDRLIATRTHTALAMLSRGCPYSCTYCTNHAFRSLYPNAKEYHRSRTPEGAIAYIQ
                     ALRTWYPEVRELRFLDNVFGISTDWLARFGEMYKREVGLPFACDHRAELLTEDTVHLL
                     ADMGCTQVYLGIESGDEALRRRALGRTMSNELLVQVNERLHAAGIRIFAYNMVGLPGE
                     TRATALLTPKLNAQARTDDACISVFSPYPSTVLYDVSIKDSSVVEPIDYTRLTFLDQP
                     AFDSNEVAFMALSFNTLRRWYRRYGTESRMGRRMDRIVLSPRLPMRFIIAVTEHWEGE
                     REMIKSFLRNRAPGVFLFVKRLLRGHVTSEPRAIAHGHQEGPPRA"
     gene            1233..2894
                     /locus_tag="VN478_06285"
     CDS             1233..2894
                     /locus_tag="VN478_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HWQ22316.1"
                     /translation="MATEMIRLHIEETGEDVLYPRGVRLDSLAKDYASYHASRIMAAR
                     VNNDIRELFKTLNHDATVRFIDMTTEDGARIYQRGLVFILYAAARDLFPARRLRVLHS
                     LGLGLFCDFREIRATDDEVSQIKQRMQELIDQDVEYQKQELYKWDALQLLENLGMHDK
                     AELLRYRSKSTVNIYYLGKHPNYFYGFMPHSTGDTPIFNLIPYRTGMVLVFPGITSPN
                     ALPDYVPQTKLADIFGEAEEWGHIIGVETAGDLNDVVAGGRTRELIMVSEMLHEKKIA
                     ALADRVTASGARLVLIAGPSSSGKTTFSKRLALQLKVNGFDPHAVSLDDYFVDRDKTP
                     LGSEGKPDFECLESLNTAKFNIDLNRILAGETVTLPKYSFKTGTSSDDGGIIVPSDRT
                     ILVVEGIHGLNPELTSQIPASAKYRIYVSALTFLNLDADNRIPTTDVRILRRMVRDHN
                     FRGYSALQTLQIWHSVRMGEERHVFPFQENADAMFNSSLVYELAFLKNYAMQLLLQID
                     NTVPEYSEARRLVRFLDYFLPVMDIDVIPSTSIIREFVGNSYFNY"
     gene            2907..3737
                     /locus_tag="VN478_06290"
     CDS             2907..3737
                     /locus_tag="VN478_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22317.1"
                     /translation="MHTDRVMQLALEMAGLTEIPEDTQVYVPGEITKALVCIDADTGL
                     LNIARDLGYDGVIVHHPQGDDAMLNLHVVMNRQLLTLTRAGVPEIEAREALLPRQFGV
                     ERNNHMRNWDYMPSVARLMGMPFMNVHYPCDLVMERIVDAHVQRRLAEMGPSATVQEL
                     TSILLELPEYAHARTRPAVVVGQPSSPAGKVVTAFAGGTNGGAAVVEAYWRHGVGTVM
                     TLHMEVADILKLRQSEVKGTLVVAGHMACDSVGLNALIRVLRANGLDLTPMGGIVPGA
                     "
BASE COUNT          746 a         1242 c         1075 g          678 t
ORIGIN      
        1 acgcatgggt caccggcagg actccacagg gcattcccgg cgtctggtgg cacaacggac
       61 aggaagtcgg ccacgaaccc gtcggtcccc tggtggcgaa cctcgacgaa ctgccgatcc
      121 ctgactttgg cctgttcgat cgtgatcggc tcattgccac ccgcacccac acggctctcg
      181 ccatgctatc gcgcgggtgt ccctatagct gcacgtattg caccaaccac gccttccgat
      241 ccctgtaccc caacgcgaag gagtaccacc ggtcgcgtac gcctgagggc gccatcgcct
      301 acatccaggc gctgcgcacc tggtatcccg aggtgcgcga actgcgcttt ctcgacaacg
      361 tgttcggtat ctccacggac tggcttgccc ggttcggcga gatgtacaag cgcgaggtcg
      421 gcctcccgtt cgcctgcgat caccgcgccg agctgctgac tgaggatact gtccatctgc
      481 tggccgacat gggctgcacg caggtctacc tgggcatcga gtcgggcgac gaggctctgc
      541 gccgccgagc cctgggccga acgatgagca acgaactcct ggtccaggtg aacgagcggc
      601 ttcacgcagc cggcatccgc atctttgcgt acaacatggt ggggctgccg ggcgagacac
      661 gcgcgacggc actgctcacc cccaagctca acgcgcaggc gcgcacagac gacgcgtgta
      721 tcagcgtctt ctcgccctat ccttctacag tcctgtatga cgtcagcatc aaggacagct
      781 ccgtcgtcga gcctattgac tacacacgcc ttaccttcct cgaccagcct gcattcgaca
      841 gcaacgaagt cgcgttcatg gctctgtcct tcaacacgct gcgccggtgg taccggcgat
      901 atggcacgga gtcgcgcatg ggccgccgga tggaccgtat cgtcctgtcg ccacgccttc
      961 ccatgcgctt catcatcgcc gtcacggagc attgggaagg ggaacgcgaa atgataaagt
     1021 ccttcctgcg caaccgtgcg cccggcgtct tcctgttcgt caaacgactg cttcgcggac
     1081 acgtcaccag cgaaccgcgc gccatcgccc acggccatca ggaaggaccg ccccgagcct
     1141 gacgtctcct ctgcaggatt cgccctcctc gggaccttcg gccttgctca gtcaccctgc
     1201 acgcgtagac tgagaagtca ggaggcggca caatggcgac agagatgatc cgcttgcaca
     1261 tagaggagac cggcgaggac gtcctgtacc cgcgaggggt ccgcctggac tcgctggcaa
     1321 aggactacgc ctcgtaccac gcgtcgcgta tcatggcggc gcgcgtcaac aacgacatcc
     1381 gtgaactgtt caagacgctg aaccatgacg ccacggtccg cttcatcgat atgaccacag
     1441 aagatggcgc ccgcatctat cagcgcggcc tcgtgttcat cctgtacgct gcagcgcggg
     1501 acctgttccc cgcccgcagg ctccgggtcc tccactcgct ggggctgggc ctgttctgtg
     1561 acttccgcga gatacgcgcg accgatgacg aggtgagcca gatcaagcag cggatgcagg
     1621 agcttatcga ccaggacgtc gagtaccaga agcaggaact gtacaagtgg gacgccctgc
     1681 aattgctgga gaaccttggg atgcatgaca aggccgagct gctacggtac cgcagcaagt
     1741 ccacggtcaa catttactac ctgggaaaac accccaacta cttctacggc ttcatgccgc
     1801 acagcaccgg cgataccccg atcttcaacc tcatccccta ccgcaccggc atggtactgg
     1861 tcttccccgg cattaccagt cctaacgctc ttccggacta cgtgcctcag accaagctgg
     1921 ccgacatctt tggcgaggcc gaggaatggg gacatatcat cggcgtcgag acagcaggag
     1981 acctcaacga cgtcgttgcc ggaggccgta cccgagaact catcatggtc agcgaaatgc
     2041 tgcatgagaa gaagattgca gcgctggccg accgcgtcac cgcctccgga gcccgcctcg
     2101 tcctcatcgc aggaccgtcc tcatctggca aaacgacctt cagcaagcgt ctggctctgc
     2161 agctcaaggt caacggcttc gacccccacg ctgtttctct cgacgattac ttcgtcgacc
     2221 gcgacaagac cccgctcgga tcagagggca aaccggattt cgagtgcctg gaatcgctca
     2281 acacggcgaa gttcaacata gacctcaacc gcattctcgc cggggagact gtcacgctgc
     2341 cgaagtacag cttcaagacc gggacctctt ccgatgacgg cggcatcatc gttcccagcg
     2401 accgcacgat ccttgtcgtc gagggcatcc acgggctgaa cccggagctg acatcacaga
     2461 taccggccag cgccaagtat cgcatctacg tgtcggccct caccttcctc aacctggacg
     2521 cagacaatcg gatcccgacg accgacgtgc gtatcctgcg gcgtatggtg cgagaccaca
     2581 acttccgcgg gtactcggcc ctgcagactc tgcagatatg gcactctgtc cgcatgggcg
     2641 aggagcggca cgtcttccca ttccaggaga acgccgacgc catgttcaac tcatcgctcg
     2701 tctacgaact ggcttttctc aagaactacg caatgcagtt gctgctgcag atcgacaata
     2761 cggtgccgga gtactccgag gcgcgtcgtc tcgtccggtt cctcgactac ttcctgcccg
     2821 tgatggatat tgatgtcatc cccagcacct ccatcatccg ggaattcgtc ggcaacagct
     2881 acttcaacta ctagaaggag gggctcatgc atacagaccg agtcatgcag ttggcactcg
     2941 agatggcggg gctcacagag atccccgagg acacgcaggt gtacgttccc ggcgagatca
     3001 cgaaggcact ggtgtgtatc gacgccgata cgggtctgct gaacatcgcc cgcgacctgg
     3061 gctacgacgg cgtcatcgtg catcacccgc agggtgatga cgccatgctg aacctgcacg
     3121 tagtcatgaa tcggcagctg ctgacgctca caagagcagg ggtgcccgag atcgaggccc
     3181 gcgaggctct gctgccacgt cagttcggtg tcgaacgcaa caaccacatg cgcaactggg
     3241 actacatgcc cagcgtcgcc cgcctgatgg gcatgccgtt catgaacgtc cactatccat
     3301 gcgacctggt gatggaacgc atcgtggacg cccacgttca gcggcgtctg gctgagatgg
     3361 gcccgtcagc aaccgtacag gagctcacat ctatcctgct cgaactcccg gagtacgcac
     3421 acgcaaggac acgcccggca gtcgtcgtcg gacagccgtc atcgccggcc ggcaaggtcg
     3481 tcactgcttt tgcgggcggg acgaatggcg gcgctgccgt cgtcgaggca tactggcgcc
     3541 acggcgtcgg gacggtcatg acgctgcaca tggaggtcgc cgacatcctg aagctgcgcc
     3601 agtcagaggt caaagggaca ctcgtcgtcg ccgggcatat ggcctgcgat tcggtgggac
     3661 tcaatgcgct catccgcgtc ctgcgggcga acgggctcga cctgactccg atgggcggca
     3721 tcgtgccagg agcgtagcgg g
//
LOCUS       DATMZD010000303         2491 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_118923,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000303 DATMZD010000000
VERSION     DATMZD010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118923"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="VN478_06295"
     CDS             complement(<1..316)
                     /locus_tag="VN478_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010916746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HWQ22318.1"
                     /translation="MEEKIIGIDLGTSNSAAAVMVGGKPTIVPAAEGVSIGGKAVPSY
                     VAFTKDGQVLVGEPARRQAALNPETTVQAIKRKMGTDYTVNMFGKTYTPQQISGFILR
                     KIK"
     gene            complement(360..1004)
                     /gene="grpE"
                     /locus_tag="VN478_06300"
     CDS             complement(360..1004)
                     /gene="grpE"
                     /locus_tag="VN478_06300"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HWQ22319.1"
                     /translation="MMNQYRFYVSSEGQGAYDGEAKDVQSSPDTPLIVVPDHEEGDGG
                     EALRMLGSRVRELQQEVERLNSAVHEEQMLRIREQATLQRDKSSFEARTKLSLFRDLL
                     AVVDSFDQLVKHGEGSPGDSSLLAGSQAVLGQLTQFLTRNGVHRIEGLEGNAYDPATS
                     EIARVVADSGAPANTVVRVLRDGYRLGEMLLRPAMVDVASEPQIPAGDSEDVED"
     gene            1211..1885
                     /gene="deoC"
                     /locus_tag="VN478_06305"
     CDS             1211..1885
                     /gene="deoC"
                     /locus_tag="VN478_06305"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HWQ22320.1"
                     /translation="MYTDQITRKELAGFMDHTLLKPDATCADIERLCEEAARLGVFAV
                     CVNSSMVETASAALTACAVRVASVVGFPLGASLSAAKARETELAIRHGASEIDMVIQI
                     GWLKERNYRGVAQDIAAVVQSAGDSLVKVIIETCLLTDEEKRAACAVAVSAGAGFVKT
                     STGFSKAGATEDDVRLMREVVGPSVGVKAAGGIRDLRTAVAMLNAGATRLGVSASPTI
                     LAEAQQ"
     gene            1893..>2491
                     /gene="recO"
                     /locus_tag="VN478_06310"
     CDS             1893..>2491
                     /gene="recO"
                     /locus_tag="VN478_06310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HWQ22321.1"
                     /translation="MVYHWTLASILGASRVGEKDKRLVLFSRELGRVTAVARGTAVPD
                     AKWSTSFEPFSLLYLRLYERSHFLTVVSSEERVVYTGILSSLSKSLKGMAMNQLAECV
                     LEPSSEEPGLFASYVTALSRLNVAPDAAEEDRAFLQFTLDVLNELGFGVSSMHCERCG
                     VALDDNVHFSMRDNIFHCHSCRATETDIVLSPELVSFLRT"
BASE COUNT          501 a          758 c          683 g          549 t
ORIGIN      
        1 tcttgatctt ccgaaggatg aacccgctga tctgttgagg cgtgtatgtc ttgccgaaca
       61 tattgaccgt atagtcggtg cccatctttc gcttgatggc ttgaaccgtt gtctcgggat
      121 taagtgccgc ctgccgtcgc gccggttccc ccacgagcac ctgcccatct tttgtgaagg
      181 caacgtagga tgggacagct ttgcccccga tgctgacgcc ttccgccgcc ggcacgatcg
      241 tcggcttgcc gccgaccatg acagctgctg cggaattcga agttccaaga tcaatgccga
      301 taatcttctc ttccatattc gacaaacctc ctcagttagg atacgttcca acaacctgat
      361 cagtcctcga catcttcgct gtcccctgca ggaatctgcg gttccgatgc gacatcgacc
      421 atcgccggcc gcagcaacat ctcccccagc ctgtagccgt cacgcaggac tctcacgacg
      481 gtgttggcgg gtgccccgga atccgcaacg acacgagcga tctcagaagt cgccgggtca
      541 taggcattgc cctccaatcc ctcgatcctg tggaccccat tcctcgtaag gaactgggtc
      601 aactgtccga ggacagcctg agaccccgcg agcaggcttg aatcgcccgg gctgccctcg
      661 ccgtgcttca caagctggtc aaaactgtcg accacggcaa gcagatcacg aaacagagag
      721 agcttggtcc tggcctcgaa ggaactcttg tcacgctgaa gcgttgcctg ttcgcgtatg
      781 cgaagcatct gttcctcatg aacggcactg ttgagacgct ccacttcctg ctgcaactcg
      841 cgtaccctgg acccaagcat cctcaatgct tctcctccat caccttcctc atggtcagga
      901 accacaatca atggtgtatc gggggaagac tgaacatctt tcgcctcacc atcgtaggcg
      961 ccttgccctt cactactaac atagaacctg tactggttca tcatctatgc gctctccggt
     1021 cagtttccac cacaaaggct gcactcagcc caagcgtctc tggacgttag cactctcact
     1081 gtacgactgc taatcgtgcc ataattgtag ttcgaaccat gttggtgtca agcgctcaat
     1141 caccacgaag cgttgatttc tggcgcagta acgcgataat gtacacgata cgaatccttg
     1201 gaggcgaccc atgtacaccg atcagatcac cagaaaggag ctcgccggct tcatggacca
     1261 tacgctcctc aagcccgacg cgacttgcgc cgacatcgaa cggctatgtg aggaagcagc
     1321 acgacttgga gtgtttgccg tatgcgtgaa ctcgtcgatg gtcgagactg cttcggcagc
     1381 cctgactgcg tgtgcggtgc gtgtcgcgtc ggtcgtcggc tttccgcttg gtgcgtctct
     1441 ctcggccgcc aaagcgcggg agaccgagtt ggccattcgg cacggagcgt cggaaatcga
     1501 catggtcatc cagatcggat ggctcaagga gaggaactac cggggtgtcg cccaagacat
     1561 cgctgcggta gtgcagtcag ccggagactc tctcgtgaag gtcatcatcg aaacgtgctt
     1621 gctgacagac gaggagaagc gcgcggcatg tgctgtcgcc gtttcggcag gagcggggtt
     1681 tgtgaagacg agcacaggtt tcagcaaggc cggcgcgaca gaagacgacg tcaggctgat
     1741 gcgtgaggtc gtcggacctt ctgtcggcgt gaaggctgcg ggaggtatcc gcgatcttcg
     1801 cactgccgtc gctatgctca atgctggtgc gactcgcctt ggggtctcag cgtctcccac
     1861 cattctcgcg gaggcccagc agtagcactc cgatggtcta tcactggacg ctcgcctcca
     1921 ttctcggcgc atcgagggtg ggagagaagg acaaacgcct ggtgctcttc tccagggaac
     1981 tgggcagggt gacagccgtc gcacggggga ccgccgtgcc ggacgccaag tggtcaacct
     2041 cgttcgagcc cttctcgcta ctctatctgc gtctgtacga acgttcgcac ttcctgactg
     2101 tcgtcagttc cgaagaacga gtggtctaca ccggtatcct gtcgtcattg tcgaagtcgc
     2161 tgaaaggcat ggcgatgaac cagctggcag aatgcgttct tgagccctca tcggaggaac
     2221 ctggcttgtt tgcctcatat gtgacagccc tctcgagact caacgttgcc ccggatgcgg
     2281 ccgaggaaga cagggccttc ctgcagttta cgctcgacgt cctcaatgag ctcgggttcg
     2341 gcgtgagctc catgcattgt gaacgctgtg gagtcgcgct tgatgacaac gtacacttct
     2401 ccatgagaga caacatcttt cactgccact cctgcagggc caccgagaca gacatcgtac
     2461 tctctcccga gctcgtcagc tttctcagaa c
//
LOCUS       DATMZD010000304         2031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_40059,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000304 DATMZD010000000
VERSION     DATMZD010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40059"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="VN478_06315"
     CDS             <1..897
                     /locus_tag="VN478_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002562837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HWQ22322.1"
                     /translation="FVISEEVQKALHKGIPVVALESAIITHGMPYPVNLELAQELEDI
                     VRKGGAVPATTAVVSGRIRVGLSKSELEMLARAKWVKKIGPRDIPVAVAMGFSGGTTV
                     AASLTIADAAGIGVFVTGGIGGVHRGVSQVLDVSQDLPVIGRSRCITVCAGAKSILDL
                     RNTLEYLETMSVLVLGYQTDTLPAFYVRDSGIQLEHRVESASEIAAIVGARDRLKLDT
                     GILVTNPIAEADQVDPKKHEKVLQAALKKAAEEGIEGKAMTPYVLAYMQKNLPGVIEA
                     NIALIKSNVALGTQVAILLKQR"
     gene            909..1814
                     /locus_tag="VN478_06320"
     CDS             909..1814
                     /locus_tag="VN478_06320"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HWQ22323.1"
                     /translation="MFFVTGDLNIDATTVAPVLSPSGKEAQAHIMLSCGGQGGNVAFF
                     LRCLGHPVQLFGTLGTDVAGDLYTLHLAHLGIQYAGDRVNTPTGMVSVIQEAGSYHMY
                     RQRGANAAVDPNAFSRFVRGTFTTGADVLFISGYSLLDASCRSVLEAILKDSLAPRPI
                     IAMDPASIDAMSSMGRETVLAMARLCDYLLPNEEEACWLAQKTDPTDAARALRRLTGT
                     TIVVKLGSGGALLCTDDGVTVAPAVALNPVDVTGAGDSFAAAFLSACVSTSDPAAALD
                     AAVRFSAAKVRLAGTQPLELLDYKG"
     gene            complement(1802..>2031)
                     /locus_tag="VN478_06325"
     CDS             complement(1802..>2031)
                     /locus_tag="VN478_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22324.1"
                     /translation="LINASLKSFQPQFVALMLLWEPILGSLAAFVVLGDRPGPAELMG
                     GAVILIGLALGIVAGGRFTREGPADTKGQPL"
BASE COUNT          396 a          654 c          605 g          376 t
ORIGIN      
        1 ttcgtcatct ctgaggaagt ccagaaagct cttcacaagg gaatcccggt ggttgccctc
       61 gagtcggcca tcatcacgca cggcatgccg tatccggtca accttgaact ggcacaggaa
      121 ctcgaggaca tcgtccgtaa gggcggggcc gtcccggcta cgaccgctgt cgtatcgggc
      181 aggatccgtg tcggtctgtc gaagtcagaa ctcgagatgc tcgcgcgagc caagtgggtc
      241 aagaagatcg ggcctcgcga catccccgtc gctgtcgcga tgggattcag tggtggtacg
      301 accgttgccg cttcgttgac gatcgccgac gcagcaggca ttggcgtctt tgtcacgggt
      361 ggcatagggg gcgttcaccg cggcgtgagt caggtcctcg atgtttccca ggacctgccc
      421 gtgatcggcc gttctcgttg catcaccgtc tgtgctggag caaagtccat cctcgacctg
      481 agaaacacac tggagtacct cgagacgatg tccgtcctcg tcctggggta ccagaccgac
      541 actctgccgg ccttctacgt gcgtgattcg ggcatccagc tggaacatcg tgtcgagagt
      601 gcgtcagaga ttgcggcgat cgtgggagcc cgcgaccgcc tgaaactcga caccggtatc
      661 ctcgtcacaa atccgatcgc cgaggcagat caggtcgatc cgaagaaaca cgagaaggtc
      721 cttcaggctg cgctgaagaa ggctgcggaa gaaggcatcg aaggcaaggc gatgacgcca
      781 tacgtcctgg cctacatgca gaagaacctg cccggcgtca tcgaggcaaa catcgctctc
      841 atcaagagca acgtcgccct gggcacacag gtcgccatcc ttctgaagca gcgctgatct
      901 caggccccat gttcttcgtc acgggcgacc tcaatatcga cgccacgaca gtggcgcccg
      961 ttctgtcgcc ctccggaaag gaagcgcagg cgcacatcat gctgtcctgc ggaggacagg
     1021 ggggaaacgt cgcattcttc ctgcgatgcc tgggccatcc cgtgcagctc ttcggtaccc
     1081 tggggaccga tgttgcagga gacctctaca ctctgcatct ggcacacctc ggcatccagt
     1141 atgccggaga ccgcgtgaac acgccgactg gaatggtgag cgtcatccag gaagcaggct
     1201 cctaccacat gtaccgtcaa cggggagcca acgcagcggt agacccgaac gccttctcac
     1261 gcttcgtgcg tggcaccttc acgaccggag ccgacgtgct cttcatctcg gggtacagcc
     1321 tgctcgatgc gtcctgccgg tctgttctcg aagccatact gaaagactcc ctggctcccc
     1381 gaccgatcat cgcgatggat ccggccagta tcgacgccat gagttccatg ggacgcgaga
     1441 cagtccttgc catggcacga ctgtgcgact acctcctgcc caacgaggaa gaagcctgct
     1501 ggcttgcaca gaagactgac ccgaccgacg ccgctcgtgc tctccgcaga ttgacgggca
     1561 caacgatcgt cgtcaagctg ggctcgggag gagctctcct gtgcacggac gatggtgtca
     1621 ccgtggcacc cgccgtcgcc ctgaaccctg tcgacgtgac tggtgccggg gactccttcg
     1681 cggctgcctt cctttcggca tgtgtctcga cgtcagaccc ggccgcggca cttgatgctg
     1741 ccgtgcgctt cagcgcagcc aaggtccgac tcgcgggcac acaacccctg gagctcctgg
     1801 actacaaggg ctgaccctta gtgtctgcag gcccctcgcg ggtgaagcgg cctcccgcga
     1861 cgatccccag cgccagcccg atgaggatga ctgcgcctcc catgagttct gcaggacccg
     1921 gccggtctcc cagcaccaca aatgctgcca ggctcccgag gatcggctcc cacagcagca
     1981 taagagctac gaactggggc tggaacgact tgagtgaggc attgatgaga c
//
LOCUS       DATMZD010000305         2066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_129188,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000305 DATMZD010000000
VERSION     DATMZD010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129188"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VN478_06330"
     CDS             complement(<1..342)
                     /locus_tag="VN478_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HWQ22325.1"
                     /translation="MLNQNDLNNIRNIGIIAHIDAGKTTTSERILYYTGSTYKLGNVD
                     DGNTTTDFLPQERERGITIQSAVVTAFWKGFKVNIIDTPGHVDFTAEVERALRVLDGG
                     VIILSGLEGVQA"
     gene            complement(376..846)
                     /gene="rpsG"
                     /locus_tag="VN478_06335"
     CDS             complement(376..846)
                     /gene="rpsG"
                     /locus_tag="VN478_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HWQ22326.1"
                     /translation="MPRKKRQFRKPAQADPIYNSELLNHFINKILKKGKKVKAQGIVY
                     GAIKLIDAKGAKPGIEVFQQAVEKCRPLVEVKPRRVGGATLQVPVEVESRRGINLSME
                     WIVDAAKKRAGKSMIEKIAYEMIDAVSETGSAFKKKSDVHRMAEANKSYSHYRW"
     gene            complement(846..1247)
                     /gene="rpsL"
                     /locus_tag="VN478_06340"
     CDS             complement(846..1247)
                     /gene="rpsL"
                     /locus_tag="VN478_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HWQ22327.1"
                     /translation="MATFNQLVRSPRVQPKFKTKTPALKGCPQKRGVCTQVKTATPKK
                     PNSALRKIARVRLTNGMEVTAYIGGIGHNLQEHSMVLIRGGRVKDLPGVRYHIIRGTL
                     DTAGVANRMKARSKYGAKRAKKGAVAKKGGK"
     gene            complement(1385..>2066)
                     /locus_tag="VN478_06345"
     CDS             complement(1385..>2066)
                     /locus_tag="VN478_06345"
                     /inference="COORDINATES: protein motif:HMM:NF016859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HWQ22328.1"
                     /translation="HIVDVKEKNYEEKKQIVVTSKAGAEAKFKVSPVTRFRVKDGAHV
                     RVGDQLTEGSIDPKGLLELVGVEETARYLVQEVQKVYRSQGVDINVKHVEMIIRQMLK
                     KVKVVEPGDTTFFTDQIVSKHELLLQNRKVRAQGGKPASFDLVLQGITKSSLETDSFL
                     SAASFQETPRVLADAAIKGKIDRLTGLKEAVIVGKSIPAGTSSPELMFEKHKMQAEAA
                     PGVRGNQI"
BASE COUNT          397 a          634 c          535 g          500 t
ORIGIN      
        1 ggcctgtaca ccctcgaggc ccgagaggat gatgactccc ccatcaagca ctctgagagc
       61 gcgttccact tctgcggtga agtccacgtg gccgggcgta tcaatgatgt tgaccttgaa
      121 acccttccag aaggcagtga cgacggccga ctgaatcgta atgccgcgtt cccgctcctg
      181 aggaaggaag tccgtggtcg tattgccgtc gtcgacgttg ccgagtttgt acgtggaccc
      241 cgtgtaatag aggattcgtt ccgaggtcgt cgtcttcccg gcatcgatat gggcaataat
      301 cccgatgttt cgtatgttgt tcagatcgtt ctgattaagc atgcagtgcc actacccttc
      361 gacagcgttc cgattctacc aacgatagtg agagtacgac ttgttcgcct ctgccatccg
      421 gtggacatcc gacttcttct tgaaggcgga gccagtctca gaaaccgcgt caatcatctc
      481 gtaagctatc ttctcgatca tcgacttgcc ggcacgtttc ttggcggcat cgacgatcca
      541 ttccatactc aggttgatgc cacgtcgaga ctcgacttca acaggcacct gcagtgtggc
      601 tccgccaact cggcgaggct tcacctcaac cagaggccgg cacttctcga cagcctgctg
      661 gaatacttcg atcccaggct tcgcgccctt cgcgtcgatg agcttgatgg ccccatagac
      721 aataccctgc gccttgacct tcttgccctt cttgaggatc ttgttgatga aatggttcaa
      781 cagctcgctg ttgtagatgg gatcagcctg agctggcttg cggaactgcc gcttcttcct
      841 gggcattact tgccaccttt cttcgctacg gcgcctttct tggctcgctt ggctccgtac
      901 tttgaacgcg ccttcatccg attggccacg cctgcggtat ccagtgttcc acgaatgatg
      961 tgatagcgca cgccaggcaa gtccttgacc ctgccgccac gaatcagcac catcgagtgc
     1021 tcctggaggt tatgaccgat accgccgatg taagccgtga cctccatgcc attcgtcaga
     1081 cgaactctgg caatcttccg caatgcggag ttgggcttct tcggtgtcgc ggtcttgacc
     1141 tgggtgcaga cacccctctt ctgtgggcaa cccttgagag ccggagtctt cgtcttgaac
     1201 ttcggctgca cgcggggaga ccgcaccagc tggttaaatg tcgccataca gtttcctcct
     1261 aaggacgtaa atacgcatct ctaaatgata cgcattaggg ggcaagtgtc aagcacttgc
     1321 ccccttgaca aatcacactc gtgaagcccc aacgccatgt atcacgcccc atggcatgcc
     1381 tacgctagat ctgatttccc cgcacacccg gcgcggcttc cgcctgcatc ttgtgcttct
     1441 caaacatgag ctctggagac gacgtgccgg caggaatcga cttgcctaca atgacggcct
     1501 ctttgaggcc ggtgagacga tcgatcttgc ccttgatagc agcgtcagca agcaccctcg
     1561 gcgtctcctg gaacgatgcg gcggacagga agctgtccgt ctccagtgaa gacttcgtga
     1621 tgccctggag caccagatcg aacgaggcag gcttgcctcc ctgcgcgcgg accttgcggt
     1681 tctgcagcaa gagttcgtgc ttgctcacga tctgatcggt gaagaatgtc gtgtcgcctg
     1741 gctcgacaac cttcaccttc ttcagcatct gacgaatgat catctcgacg tgtttcacgt
     1801 tgatgtcgac accttgagag cgatagacct tctggacttc ctgaaccagg tagcgggcag
     1861 tctcctcgac gccgacaagt tcaagcagcc ccttcgggtc gatcgagccc tcggtcagct
     1921 ggtcgcccac gcgcacgtgc gcgccatcct tgacgcggaa cctggtcacc ggcgagactt
     1981 tgaacttcgc ctcggctcct gccttcgatg tgacaacgat ctgcttcttc tcctcgtaat
     2041 tcttctcttt cacgtcgacg atatgg
//
LOCUS       DATMZD010000306         3236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_9041,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000306 DATMZD010000000
VERSION     DATMZD010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9041"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VN478_06350"
     CDS             <1..270
                     /locus_tag="VN478_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013300357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWQ22329.1"
                     /translation="RLCCSLSGGEKQRVALARALIAKPDIILADEPTGNLDLSNGEKI
                     VEVLHEANRQGATIFMATHDLYLVQSTRLPFVRLRGGRKVEEGNF"
     gene            272..1174
                     /locus_tag="VN478_06355"
     CDS             272..1174
                     /locus_tag="VN478_06355"
                     /inference="COORDINATES: protein motif:HMM:NF037183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HWQ22330.1"
                     /translation="MFTLKQTLRDLERHPVRTLVVSILIAVLMVASAGIYAMSIDVRH
                     TISEIESTHSINAYLLRGLDQTSIQTLLDEMKKDEAVARAEYVSPADGLKKLEAQYPQ
                     FANVFSDLTRNPIPPLIRVYPVSVQGIASLAAELRSLAGVQTVEYDEGSVQGMANANA
                     FVQSLLLYVLILAGLLFVAGFCLMAFSIINGKRKETAVLSMVGASSLQVLLSTPAHLV
                     ALWTLASLLFAAVLPSVISYSTARLKASFSWITPEPAGAVYAGAASVVLLASLACLLL
                     ACVIATVLAYRIDEEQRREETLLE"
     gene            1171..2349
                     /locus_tag="VN478_06360"
     CDS             1171..2349
                     /locus_tag="VN478_06360"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HWQ22331.1"
                     /translation="MRVSRLLVLIVLILGLSVGASVFAPGVEGDLTSDLKAAQQKMDK
                     LKAQLQEIQKLIKENKSAKYKLLSQLTSLNDQVDQLEGDMGDLDSRIEQLKSLLSKTA
                     AQIAVREKEYGELSAGTESSIDLLYHVSNLDVAGLPFTGGDLSSTVEAQQGVSAVLQQ
                     ITTSLSAVSTQKLALQSDRTDYVNTKSQLEQVYALKKQQASLLAQQKAATQQTLNYLA
                     AKGSQYSADDRRIQEEITRENKLIDQIVAEIDRQQHPGNEPTGKLLWPIPGTIRITEL
                     FGMRHDPINGVWEMHNGIDMGCPMSTTLRAPADGVVVYEGRFSGYGNMLMLQCGQHMT
                     LLFGHLKSFIAKKGQSVKRGQVIALTDNTGWSTGPHLHFEVRIDNTPKNPMLYLGPKP
                     "
     gene            2374..>3236
                     /locus_tag="VN478_06365"
     CDS             2374..>3236
                     /locus_tag="VN478_06365"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HWQ22332.1"
                     /translation="MPSRRSSLIIAIAALLVVIMAFWAGFVAYPFLAPRLTGSSRPAS
                     VQTTIDRDTYLKLSGELYDTLSLQYYQKVDAAKLLKGQVAGLGDPYTELFTPAEAADF
                     REQLSGEYVGIGVVISPSKKADVIEIVRVFAGTPAEEAGLKPGDLIDSIDGTTARNQD
                     LDVVSARVKGKAGTSVRLKIDRAGTLLDYTVVRRQVELPVVESKVIGNKVGYISVSSF
                     SSGVAVKFARALHALEDQKVSGLVIDIRDNGGGYLNECLDMLSNFIPHGTALWTREAG
                     GEVNPVTVNGN"
BASE COUNT          715 a          952 c          908 g          661 t
ORIGIN      
        1 cgcctgtgct gttcgctgtc gggaggtgag aagcagcgtg tcgctctcgc gcgcgccctt
       61 atcgcaaaac ccgacatcat ccttgccgac gagccgaccg ggaacctcga tctgagcaac
      121 ggagagaaga tcgtcgaggt cctccatgag gccaaccgtc agggcgctac gatcttcatg
      181 gcaacacacg acctgtacct cgttcagtcg acccgactgc cgttcgttcg actccgtggc
      241 gggcgaaagg tagaggaggg caacttctga catgttcact ctgaagcaga cacttcgaga
      301 tcttgagaga caccccgtac gtactcttgt cgtgtcgatc ctgatagccg tgctgatggt
      361 cgcgagcgca ggtatctatg caatgtcgat cgacgtacgc cacacgatct cagagattga
      421 gagcacacac tccatcaacg cgtatctctt gcgcggactc gatcagacat ctattcagac
      481 tcttctcgac gagatgaaga aggatgaagc cgtggcacga gccgaatacg tgagccctgc
      541 agacggcctg aagaagctcg aagcccagta tcctcagttt gcaaacgtgt tcagcgacct
      601 gacacgcaac ccgattccgc ctctgatccg cgtgtatccc gtctccgtac agggcatcgc
      661 tagcctggct gccgaacttc gttctctcgc gggtgttcag actgtggagt atgacgaggg
      721 ctccgtgcag gggatggcga acgcaaacgc ttttgtgcag agcctcttgc tatacgtgct
      781 cattctcgcc ggcctcctgt tcgtggccgg cttctgtctc atggcgttca gcatcatcaa
      841 tggcaaacgt aaggagactg cagtgctgag tatggtgggc gccagcagtc tccaggtact
      901 tctctcgacc ccagcccacc ttgtagctct ctggactctc gcgtctctcc ttttcgccgc
      961 cgttcttccg agcgtcatca gctactccac tgcgcggctc aaggcgtcgt tctcctggat
     1021 cacgcctgag ccggcgggtg ccgtctacgc tggagccgcc tccgttgtcc tgctggcttc
     1081 gctcgcctgc ctgcttcttg cgtgtgtcat agctacggtg ctggcatacc gcattgacga
     1141 ggaacagcgc cgagaggaga cactgctcga atgagagtct caaggcttct cgttctcatc
     1201 gttctgatac tgggtctctc tgtcggcgcc tccgtcttcg cccctggtgt cgaaggagac
     1261 ctcacgagtg atctcaaggc tgctcagcag aagatggaca agctcaaagc gcagttgcag
     1321 gagatccaga agctgatcaa ggagaacaag tcggcgaagt acaagctcct gagtcagttg
     1381 acttccctga acgaccaggt cgatcagctg gagggagaca tgggcgacct tgacagccga
     1441 atcgaacagc tcaaatccct cctcagcaag acggcagctc agatcgccgt gagagaaaag
     1501 gaatacggcg aattgtctgc agggaccgag tcatcgatcg acctcctcta ccacgtgtcc
     1561 aatctcgacg tggcaggtct tcccttcacc ggcggagatc tcagttctac cgtggaggcc
     1621 cagcagggcg tatcagccgt tttgcagcag attaccacgt ctctgtcggc agtgagtacg
     1681 cagaaactcg ccctccagtc ggaccgcacc gattatgtca acacgaagag ccaactggag
     1741 caggtatacg cgctcaagaa acagcaggca agccttctcg cccagcagaa agccgccacg
     1801 caacagactc tgaactacct tgcagctaag ggcagtcagt actcggctga cgataggcgc
     1861 atccaggaag agatcacacg agagaacaag ctgattgacc agattgtcgc cgaaatagac
     1921 cgacagcagc atcccggcaa tgagccgact ggtaagctcc tgtggcctat tccaggtacc
     1981 attcgcataa cggagctctt tgggatgcgt cacgacccga tcaacggcgt ttgggaaatg
     2041 cataacggca tcgacatggg ctgtccgatg tcgacgaccc ttcgcgctcc tgctgacggc
     2101 gtcgtggtgt acgaggggag attctcggga tatggcaata tgctgatgct tcagtgtggt
     2161 cagcatatga cgctgctctt cgggcatctc aagtcattca ttgccaagaa gggtcagtca
     2221 gtgaaacgtg gtcaggtaat agccctcacc gacaacaccg gctggagtac cggtccccat
     2281 ctgcactttg aggtccgcat cgacaacacc ccgaagaatc ccatgctcta cctcgggcca
     2341 aaaccgtaac acctgcaacc agaggagacg cccgtgccat caagaagatc ttcgctcatc
     2401 atagcgatag cagcactgct ggttgtgata atggcattct gggccggatt tgtggcctat
     2461 ccgtttctgg ctcctcggct cacaggttcc tcccgaccgg cctcggtcca gacgaccatc
     2521 gaccgcgaca cctatctgaa gctctcgggc gagctgtatg atacgctcag cctgcagtac
     2581 tatcagaaag tcgatgcggc caaactactc aagggacaag tggcaggact aggtgacccg
     2641 tacactgagc tgttcacgcc cgctgaggcg gcggactttc gcgagcagtt gtcgggtgag
     2701 tacgtgggca tcggtgtcgt catctcgccc agcaagaagg ctgacgtcat agagatcgtc
     2761 agagtattcg ccggtacgcc tgcagaggaa gccggactca agcctggaga ccttatcgac
     2821 agcatcgatg gcacgacagc ccgaaatcag gatctcgacg tggtctcagc ccgggtcaag
     2881 ggcaaggccg gtacctctgt gcgactgaag atcgatcgag caggaacgct gctggactac
     2941 accgttgtgc gacgtcaagt ggaactgccg gtcgtcgaga gcaaggtcat cggaaacaag
     3001 gttggctata tctcagtctc ctctttcagc tccggcgtgg ccgtgaagtt tgccagagcc
     3061 cttcacgcac tggaagacca gaaggtcagc ggactcgtga tcgacatacg ggacaacggc
     3121 ggcggatatc tcaacgagtg cctcgacatg ctgtcgaact tcatcccgca cggcacggct
     3181 ctctggacaa gggaagcagg aggcgaggtg aaccccgtga ccgtcaacgg taacag
//
LOCUS       DATMZD010000307         3005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_148919,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000307 DATMZD010000000
VERSION     DATMZD010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148919"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="VN478_06370"
     CDS             complement(<1..450)
                     /locus_tag="VN478_06370"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HWQ22333.1"
                     /translation="MSSFPRVVVDLDCLTDNAAKVRSLCQEHGLGVVAVTKGVTSDAE
                     IVQAFMRGGIHSIGDSRVHDFLSLRQEEFGVLELTLLRQPPRSQIHLVPHITDRAFMS
                     ELSAMEALGAAALAKGTTCDVLAVVEMGDRRDGVPISELTSFVLAASK"
     gene            complement(635..1684)
                     /locus_tag="VN478_06375"
     CDS             complement(635..1684)
                     /locus_tag="VN478_06375"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HWQ22334.1"
                     /translation="MRIGITYNLSTEVAAAEGVPSDRADEFDSLETVQNIEKGIIAAG
                     HEPVRLGFGLDAVARLKRQPVDLVFNIAEGLYGRARESQMPAILDMMGIPYTFSDTLA
                     LSLALDKTITKQILMQHGINTPAFWSVDNPVQVEAIIQSGAPKYPLFVKPACEGSSMG
                     INDNSRVMSDDELRNQVGDLLTAYPGSSILIEEFLPGREVTVGVIGNREPEILGVMSF
                     RLLTGTDRDFFYTPEIKHHWEKYLVPECPADLSAEQYQKVRDLAVGVFKALHCRDCAR
                     IDMRFDANGEPSFMEINPLAGLTKGKSDIPVMAELMGLPYEDLIGRIIQAAVERIDAE
                     RPETTMVTEAQREAV"
     gene            complement(2064..>3005)
                     /gene="surE"
                     /locus_tag="VN478_06380"
     CDS             complement(2064..>3005)
                     /gene="surE"
                     /locus_tag="VN478_06380"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HWQ22335.1"
                     /translation="GVLAEHYDLTIVAPEGNQSGSSHSLTLGDILPIREVTMSCGLKG
                     FALRGTPTDCVLVALLDVMKDNPPDIVVSGCNHGPNVGVDVLYSGTVSAALEGLRQGH
                     PSIALSLDMVHGVQPHHFETAAAVLLEILAEPAFYADLVKAPAILNVNVPNIPSSDVR
                     GVMITKQGFRSYENFVEKQFSPRGRAYYWIAGNSGPHDAKPGSDGYALISDYVSVTPI
                     NLDLTCDELLKPLEARKGLVEKRLALVTGRRGTFGRPTTVTVSTRRSVKVAPVTASPV
                     STPEAHPTASSAPSRHAGSGSEVKAVSRAGAGKRRKG"
BASE COUNT          586 a          905 c          896 g          618 t
ORIGIN      
        1 cttcgatgct gccagtacaa aggaggtcag ttccgagatc ggcacgccgt cgcgacggtc
       61 gcccatttcg actacagcga gcacatcaca ggtcgttccc ttggccaggg cagccgcccc
      121 cagcgcctcc atggcgctca gctccgacat gaacgcccgg tccgtgatat gtgggaccag
      181 atggatctgc gagcgaggag gttgcctcag gagcgtgagt tccagaacgc cgaactcctc
      241 ctggcgcaga ctgaggaagt cgtggactcg tgagtcgccg atactgtgaa tgccgccgcg
      301 catgaatgcc tggacgatct cggcatcgga cgtcacgccc ttggtgactg ccacaacacc
      361 aagcccgtgt tcctgacaga gcgagcggac cttggccgca ttgtcggtca ggcaatcgag
      421 gtcaaccacg actcgcggaa agctactcac tgcatgcacg ctcccgtcga cagtgcctga
      481 ttcgactcac cacactgcga cgcatccagt gtagcctcgc cgttcgcgat gggaagactg
      541 gctcgtctgt ccacccggat ttgtgccgca caagaacgaa atggacgccg gctttcgccg
      601 gcgtccatcg gtagtgtcct ggaatggaga gagcctagac ggcctcgcgc tgagcctcgg
      661 taaccatcgt cgtctcgggc ctctccgcgt cgatgcgctc gacagcagcc tgaatgatcc
      721 ggccgatgag gtcctcatac ggcaacccca tcaactcggc catcacaggg atatcgctct
      781 tgcccttggt cagtccggcc agcgggttga tctccatgaa cgacggctcg ccatttgcgt
      841 cgaatcgcat gtcgatacga gcgcaatcgc ggcaatgcag tgccttgaac acgcccacgg
      901 cgagatcgcg aaccttctgg tattgttcgg cgctgaggtc ggccgggcat tcggggacaa
      961 ggtacttctc ccagtgatgt ttgatctccg gcgtgtagaa gaagtccctg tcggttcctg
     1021 tcagcagcct gaaggacatg accccaagga tctcaggttc ccggttgccg atgacaccta
     1081 ccgtgacctc gcgtccgggc aggaactcct cgataaggat gctcgagcca ggatatgcgg
     1141 tcaacaagtc gcctacctga ttgcgaagtt catcgtcgga catgacccgg gagttgtcgt
     1201 tgatgcccat gctggaaccc tcgcacgccg gcttgacgaa cagcggatac ttgggagcgc
     1261 cactctggat gatggcctcg acctgaaccg ggttatccac cgaccagaag gccggcgtgt
     1321 tgatgccgtg ctgcatcagg atctgtttgg taattgtctt gtcgagtgcc aatgacagag
     1381 ccagcgtgtc ggagaacgta taggggatcc ccatcatgtc gaggatggcc ggcatctgcg
     1441 attcgcgagc acgaccatac agaccctcgg cgatgttgaa taccagatcg accggctgcc
     1501 gcttcagtcg ggcaacggcg tccaatccaa acccgagccg cactggctcg tgcccggctg
     1561 ctatgatgcc tttctcgatg ttctggactg tctccaggga atcgaattcg tccgccctgt
     1621 cggatgggac tccttcagcg gctgcgactt cagtagacag attgtaggta atgcctatgc
     1681 gcatgataca ttctcctggt tgttggatgg gtgatgatga gtaggactct gctcatgact
     1741 atccagaccg gccatcgccg aggagcgtgg agtacggccg aaaccgccgc tcgtcagcgg
     1801 ggagaatcaa tgctggaagg ctgttaccga agggaagaga acgtgaaaac ggaccccatc
     1861 tatgcccttt gcgggctggg ggtggttcgt cgacagactc gacgtcttcg tacgaaagaa
     1921 ccattcttgc atctgtgttc acgcagtcgt ctcccatgaa gcaagttaac ttcgcacaaa
     1981 ctatagtggg gtcgattctg ttgtcaatag cttcggcccc acttgtcacg cgtactgtgt
     2041 gagactctga acagtgctct aggtcagcct tttcgcctct tgccagcccc cgcacggctc
     2101 acagccttca cttccgaacc ggacccggcg tggcgcgacg gcgcgctcga agcagtagga
     2161 tgggcttctg gagtgctgac cgggctggca gttaccgggg cgaccttcac acttcgcctg
     2221 gtcgagaccg tcacagtcgt cggcctgccg aacgttccgc gcctgccggt cacgagggcc
     2281 agccgcttct ccaccaagcc cttgcgggct tcgagtggct tcaacagctc atcgcacgtc
     2341 aagtcgaggt tgatcggcgt tacggagaca tagtcgctga tcagtgcata gccatcgctg
     2401 ccgggcttgg catcgtgggg accggaattg cccgcgatcc agtagtaggc gcggccacgc
     2461 ggactgaact gcttctccac gaagttctcg taggacctga acccctgctt ggtgatcatg
     2521 acgcccctga cgtcactcga cgggatgttg ggaacgttga cattgaggat ggcgggtgct
     2581 ttgacgagat cggcgtagaa agcgggctcg gccaggatct ccaggagcac tgctgctgct
     2641 gtctcgaagt ggtggggctg gacgccatgc accatgtcca gactgagcgc gatcgatgga
     2701 tgcccctgac gcagtccttc gagagcggca ctgacggtcc cactgtagag gacgtccact
     2761 ccgacgtttg gcccatgatt gcacccgctg accacgatgt ctggtgggtt gtccttcatt
     2821 acgtcgagca gggccaccag cacacagtca gtgggagtcc cacgcagagc aaaacccttc
     2881 agaccgcacg acatggtcac ttcgcggatc ggcaggatgt cgcccagcgt cagcgagtgg
     2941 ctggatccac tctgattgcc ttccggggca acgatcgtga ggtcgtagtg ttccgccagt
     3001 acacc
//
LOCUS       DATMZD010000308         2225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_89441,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000308 DATMZD010000000
VERSION     DATMZD010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89441"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1067
                     /gene="mltG"
                     /locus_tag="VN478_06385"
     CDS             <1..1067
                     /gene="mltG"
                     /locus_tag="VN478_06385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HWQ22336.1"
                     /translation="RLWPEARTDMTQDAQNVHEENRPTSGQSRRRLRSTVAGTVGVLL
                     VLAIVAGAVAWVRFVRIVPVEGPDAYVDVRPGDSVTAVSQRVFELGMTSAPQTLRWYW
                     RLKRGGSLREGRYVLGGVSDMETLYQLLVAGSPLTARVTFPEGYTVQQIAARLQEQAG
                     IQAVDFLKAARDAQGALLEGHLFPDTYEFLYAGDAAGVVARMLGRFAEVLPSDWAARA
                     ARRSLTSDQLVIVASIVEREAKYDADRPLVASVIFNRLKAGMLLQMDATLGYVLPSHD
                     GFYTYDELKFESPYNTYVHPGLPPTAICNPGLASLDAAARAPVSTYYYFLAKPDGHCV
                     FAKTYAEHARNMRLYLSGGN"
     gene            1069..1692
                     /gene="udk"
                     /locus_tag="VN478_06390"
     CDS             1069..1692
                     /gene="udk"
                     /locus_tag="VN478_06390"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010021558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HWQ22337.1"
                     /translation="MTRPILVGIAGGSGSGKTTVAEKIMHETAQPVVFLKQDSYYRNF
                     DGMSIEEKRKINYDHPSAYDDDLLIEHLQRLDRGEAVDVPVYSYARYEREPWTEHVEP
                     RPVIILEGILVLENAALRDLLDIKLYVDTDADVRFIRRLLRDTRERGRTVESVVAQYM
                     EVVRPMHLQFVEPTKRYADLIIPEGGFNLVAIDVIVSKLQHFLNSVG"
     gene            1701..1919
                     /locus_tag="VN478_06395"
     CDS             1701..1919
                     /locus_tag="VN478_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22338.1"
                     /translation="MARFSVPNGDVWFVQAVLTDCEGQAVVSLGERDADRSTMSVLYD
                     DSAADDLKPLFAYLTSVGKMVPVSYTKP"
     gene            1959..>2225
                     /locus_tag="VN478_06400"
     CDS             1959..>2225
                     /locus_tag="VN478_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22339.1"
                     /translation="MRRQVEELVGRRDYDAALRFAVHEADSAYGRQRYAQGIDILNTL
                     VQLLKDRQVALWNIYIGAYQKIVGLDNQLKDKSATIRDLIELSRY"
BASE COUNT          416 a          675 c          717 g          417 t
ORIGIN      
        1 gtcggctgtg gcccgaggca aggactgaca tgactcagga cgcgcagaac gtacatgagg
       61 agaacagacc gacgtccggg cagtcccggc gcaggctgcg ctcgacagtc gcgggcacgg
      121 tgggggtcct gctcgtcctg gcaatcgtcg caggtgccgt ggcgtgggtc aggttcgtcc
      181 gcatcgtgcc ggtcgaagga ccggacgcgt acgtcgacgt ccgtcccggc gacagcgtaa
      241 cggcggtcag tcagcgcgtg ttcgagcttg gcatgacgtc cgccccacag actctgcgct
      301 ggtactggcg gctgaagcgc ggcgggtccc tgcgcgaggg tcgctacgtg ctgggcgggg
      361 tcagcgacat ggagaccctg taccagctcc tggtggccgg ttcacctctc acggcgcgag
      421 tgacctttcc cgaggggtat acggtccagc agatcgctgc gcggctccag gaacaggccg
      481 gcatccaggc agtggacttt ctgaaggctg ccagggacgc tcagggtgcg cttctcgagg
      541 gacacctctt cccggacacc tacgagtttc tctacgcagg cgacgccgcg ggggtcgtcg
      601 cccggatgct tggcaggttc gcggaggtcc tgccttccga ttgggcggcc cgggcagcga
      661 ggcggagtct gacgagtgat cagctggtca tcgtcgcttc gatcgtcgag cgcgaggcca
      721 agtatgatgc agaccgcccc ctggtggcca gcgtcatctt caaccgcctc aaggcaggca
      781 tgctgctcca gatggacgcc acgctcggct acgtcctccc cagccatgac ggtttctata
      841 cctacgacga actcaagttc gagtcaccct acaacacgta cgtgcatccc ggcttgccgc
      901 cgaccgccat ctgcaatccg gggctggcgt cgctggatgc tgctgctcgc gcccctgtga
      961 gcacctacta ctactttctg gccaagcccg acgggcactg cgtcttcgcg aagacctacg
     1021 ctgaacacgc ccgcaacatg cggctctact tgtctggagg caactgacat gacacgtcca
     1081 atcctggtcg gtatcgccgg cggttcgggt tcgggcaaga cgaccgtcgc ggagaagatc
     1141 atgcacgaga ccgcacagcc ggtcgtcttc ctcaagcagg attcgtacta caggaacttc
     1201 gatgggatgt ccatcgagga gaagcgcaag atcaactacg atcacccgag cgcctatgac
     1261 gacgatctgc tgatcgagca tctccagcgt ctcgaccggg gtgaagcggt cgacgttccc
     1321 gtctacagct acgcccgcta tgaacgggag ccgtggaccg agcacgtcga gccccggccg
     1381 gtcatcatcc tggaaggcat cctggtgctg gagaatgccg cactgcgtga cctgctcgac
     1441 atcaagctgt acgtcgacac ggacgctgac gtccggttca tccgccgcct gctcagggac
     1501 acgcgtgaac gggggcgaac ggtcgagtct gtcgtggcac agtacatgga ggtggttcga
     1561 ccgatgcacc tgcagtttgt cgagccgacc aagagatatg cggacctgat tatcccggag
     1621 ggaggcttca acctggtcgc catcgacgtc atcgtcagca agttgcagca ctttctgaat
     1681 tctgtgggct gatggaggtc gtggccaggt tcagcgtgcc gaatggagac gtgtggttcg
     1741 tccaggctgt cctgaccgac tgtgagggac aggccgtggt gtcgcttggc gagcgtgacg
     1801 cagacagaag cacgatgagc gttctgtacg acgactctgc cgcagacgac ctgaagccgt
     1861 tgtttgcata tttgacgtca gttggtaaga tggtgccagt ctcatacacc aagccttgat
     1921 ggaggggagc ggaattggcc aattctgacg aggagaggtt gcgcagacag gtcgaggagc
     1981 ttgtgggccg tcgtgactat gacgcggccc ttcgtttcgc cgtgcacgag gctgacagtg
     2041 cctacgggcg gcagcgctat gcccagggca tcgacatcct caacaccctc gttcagctgc
     2101 tgaaggaccg ccaggtcgcg ctgtggaaca tctacatcgg tgcgtaccag aagatcgtcg
     2161 gtctcgacaa ccagctcaag gacaagtcgg cgacgatacg agacctcatc gaactgtccc
     2221 ggtac
//
LOCUS       DATMZD010000309         2288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_119143,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000309 DATMZD010000000
VERSION     DATMZD010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119143"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="VN478_06405"
     CDS             <1..612
                     /locus_tag="VN478_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012269216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HWQ22340.1"
                     /translation="VLGGTQSLHTNSRDEALSLPSEDSVRIALRTQQIIGYESGVADT
                     VDPLAGSYFVESMTNRIEEEAWKYISKIDELGGAVKAIERGFIQQEIQNSSYQYQLEI
                     ESGKKVIVGVNKFQVQDKPFKNLMHIDSALYEKRHKELDAMKSHRNAVKVEAALAQVK
                     AVAAGDANMMPAIIDAVDAYATLGEVCDVLRSVFGEYRPDASF"
     gene            623..1024
                     /locus_tag="VN478_06410"
     CDS             623..1024
                     /locus_tag="VN478_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HWQ22341.1"
                     /translation="MEQKIRVLVAKPGLDGHDRGAKVLARAFMDAGMEVIYTGIRRTP
                     EQIAEVAVQEDVDVVALSCLSGAHNTLFPKVVDLLKEKGANDILVLGGGVIPEDDIPF
                     LKEHGIVEVFGPGTALATCVDYIRSHVKKEA"
     gene            1029..1967
                     /gene="meaB"
                     /locus_tag="VN478_06415"
     CDS             1029..1967
                     /gene="meaB"
                     /locus_tag="VN478_06415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HWQ22342.1"
                     /translation="MDVKLGVLAGDTRAIARAITWVENGDPRGAEVIRGVYGSTGRGY
                     IVGLTGSPGAGKSTLVSRLAALLAARGQKVGILAVDPSSPFSGGAILGDRVRMFDVAL
                     TRDVYIRSMGTRGSLGGLNRATRMAARILDAAGFDWILVETVGAGQSEVDVHDVAYTT
                     VVVMAPGMGDDIQAMKAGIMEIGDLFVINKADREGANRTATQVESVLQLSMTTGWNPP
                     VLKVSAEMNTGVESVMETIERHHAHLMASGELDAIKIRMARLDVLEILKARLADTMKQ
                     QLDQPAVQVELEKVASKQSDPYSLADIIFEQSWRNI"
     gene            1964..>2288
                     /gene="mce"
                     /locus_tag="VN478_06420"
     CDS             1964..>2288
                     /gene="mce"
                     /locus_tag="VN478_06420"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HWQ22343.1"
                     /translation="MSTKNIDHIGIAVSNIQESLSFWQASLGIEPHGVEEIADRKLRT
                     AFLPVGDTNIELLEATGPESTIAKFIEKRGEGLHHIAIRVDDIDAALAELKAKGVQLI
                     DETPRN"
BASE COUNT          504 a          639 c          749 g          396 t
ORIGIN      
        1 gtcctgggtg gcacccagtc actgcacacg aacagtcgcg atgaagctct ctcgctgcca
       61 tcggaggact cggtccgcat tgcgctcagg actcagcaga tcatcgggta tgagtccgga
      121 gtggccgata ccgtcgaccc gctcgcgggg agctacttcg tagaatccat gaccaaccgg
      181 atcgaggaag aggcctggaa gtacatctcc aagatcgatg agctcggcgg tgcagtgaag
      241 gcaatcgaac gcgggttcat ccagcaggag atccagaaca gcagctatca gtaccagctg
      301 gaaatcgagt caggcaagaa ggtcatcgtc ggcgtcaaca agttccaggt ccaggacaag
      361 ccgttcaaga acctcatgca catcgactcc gctctctacg agaagaggca caaggagctc
      421 gacgcgatga agagccatcg caatgcggtc aaggttgaag ctgcgctggc tcaggtgaag
      481 gccgtggccg ccggcgatgc aaacatgatg cctgcgatca tcgacgcggt cgacgcctat
      541 gcgaccctcg gcgaagtctg tgacgtgctg cgctccgtgt tcggcgaata tcggccggac
      601 gcgtcgttct agggaggaga ccatggaaca gaagatcaga gtcctcgtgg caaagcccgg
      661 cctggatggg cacgatcgtg gagccaaggt gctcgccagg gccttcatgg atgcaggcat
      721 ggaagtcatc tataccggga tccgccgcac ccccgagcaa atcgcggagg tggccgtgca
      781 ggaggatgtc gacgtggtcg ccctctcatg cttgtcagga gcccacaaca cgctcttccc
      841 caaggtggtc gatctcctga aggagaaggg agccaacgac atcctggtgc tgggtggagg
      901 ggtcatcccg gaagacgaca tcccattcct caaagagcac gggatcgtcg aggtgtttgg
      961 accagggact gcgcttgcaa catgcgtgga ttacattcgg agccacgtga agaaggaagc
     1021 ctagtctggt ggacgtcaag ctgggagtcc tggccggcga tacgagagcg attgcgcgcg
     1081 ccatcacgtg ggtcgagaac ggggatccaa ggggcgccga ggtcatccgg ggcgtctatg
     1141 gaagcacggg gcgtggctac atcgttggac tgaccggttc acccggcgcg ggcaagagta
     1201 cgctggtgtc gcggttggcg gcattgctgg ccgcccgtgg acagaaggtc ggcattctgg
     1261 cggtcgatcc cagcagtccc ttttcaggcg gagcgatcct gggtgaccgg gtccgcatgt
     1321 tcgacgtcgc cctcacgcgt gacgtctaca tccgcagcat ggggacccgg ggttcgctcg
     1381 ggggtctcaa ccgcgccacc cggatggcgg cccgtatcct tgatgcggcg ggattcgact
     1441 ggatcctggt cgagaccgtc ggggcaggtc agtccgaggt cgacgtccac gacgtcgcgt
     1501 atacgacagt cgtcgtcatg gcgcctggta tgggtgacga catccaggcc atgaaagcag
     1561 gcatcatgga gattggcgac ctgtttgtca tcaacaaggc ggaccgcgag ggagcgaacc
     1621 ggaccgcgac gcaggtcgag tctgtcctgc agctatcgat gacgacgggc tggaatccgc
     1681 ccgtactcaa ggtatcggcg gagatgaaca ctggtgtcga gagcgtcatg gagacaatcg
     1741 aacggcatca cgcgcatctc atggcctctg gagagctgga cgcaatcaag atacggatgg
     1801 ccagactgga cgtcctggag atcctcaagg cgcgtctggc agacacgatg aagcagcagc
     1861 tggaccagcc ggccgttcag gtcgagctgg agaaggtcgc cagcaagcag agcgacccgt
     1921 actcgctggc agacatcatc tttgagcaaa gctggaggaa catatgagca cgaagaacat
     1981 cgatcacatc ggaattgctg tcagcaacat acaggagtcg ttgtcattct ggcaggcgtc
     2041 gctgggcatt gagccccacg gtgtcgagga gatcgctgac cggaaactca gaacggcatt
     2101 cctgcccgtt ggagacacga acatcgagct actcgaggct accggccccg agagtactat
     2161 cgccaagttc atcgagaagc gcggcgaggg gctgcatcac attgcgatcc gagtggacga
     2221 tattgacgca gctctcgcgg agctgaaagc caagggtgtc cagttgatcg acgagacacc
     2281 ccgaaacg
//
LOCUS       DATMZD010000310         2438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_149070,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000310 DATMZD010000000
VERSION     DATMZD010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149070"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1107
                     /locus_tag="VN478_06425"
     CDS             <1..1107
                     /locus_tag="VN478_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22344.1"
                     /translation="IQLRSGAHQELAAAEIRLIDQYLVFAEERASRFDDKQAIAERIL
                     KARQYIGKLGPRVAHSTSTGDSTRDPVLAILLPSLDEAGIKEAQDHLERETETLKHQL
                     SETAARIDASRSTGDVVAEVLGASLARRLDQATIDEWMSRDFPRLHSFFPGYRLKSVP
                     VLGDDLGARPAYDDPEAVALKGASLDHRQTVCPIVPSHRWLSPEIALMMTYFPGKAYV
                     QTAISSWDPCLAFHVQRDTFIAALSLFELQNIANVDGMLTPQNSLLTTLQLLLEDAIA
                     GLDIEILQNRLSHHDTSAFLQERVPLPPDAASAFSSIATFDYGVSLARFVYRSHFREM
                     RRVSRTSNKKISWASFYQSIFEQGTFDVAQLRDS"
     gene            1104..1787
                     /locus_tag="VN478_06430"
     CDS             1104..1787
                     /locus_tag="VN478_06430"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HWQ22345.1"
                     /translation="MNGEPQSGTADTGRPLFLAIAGNIGVGKSTLTRRLADKWGLTLL
                     PEPVKQNPFLKLYYDDPKRWGLQSQLFFLTQRVRLFKEAGALACDKIVDRTLYEDAEI
                     FARTALPHIEYQVYAQLYEYLLANLPVPDLVIYLRASTRTLSQRIQSRGRSFEKSLPS
                     SYLRRLNERYDAWARSFLRAPLIAIDTDDLDLFSLFGEYEKVTDSIADALLQKGLTWR
                     HTTTIPRRS"
     gene            1751..>2438
                     /locus_tag="VN478_06435"
     CDS             1751..>2438
                     /locus_tag="VN478_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWQ22346.1"
                     /translation="MAAYDYDTQAFLSIFPETLQQSIERLDRFPELVEVVLDLGRVPE
                     ARFEFDFVPLNENHVTRGDIDSVVRKVGEFDRDNRAGIERTLHRISAIRNRHNDIVGL
                     TCRVGRALYGKIEIIEDLVKKGSSILILGRPGIGKTTLLREASRYISDTLKKRVIIVD
                     TSNEIGGDGDVPHPGVGSSRRMQVPLNKEQHDIMIEAVENHNPEVIVIDEIGRIEEAQ
                     ACRTIAERGVE"
BASE COUNT          541 a          735 c          675 g          487 t
ORIGIN      
        1 attcagctgc gttcgggcgc tcatcaggaa ttggctgctg ctgagattcg cctcatcgac
       61 cagtacctcg tgttcgcaga agagagggcc agtcgattcg acgacaaaca ggctattgcc
      121 gagcggatac tcaaggcccg acaatacata gggaaactcg gaccacgagt cgcccattcc
      181 acgagcacgg gcgattccac acgcgatcca gtgcttgcca tcctgctccc ttcgcttgac
      241 gaggctggta tcaaggaggc ccaggaccac ctggaacgcg agacggaaac gctgaagcac
      301 cagctgagcg aaacggccgc ccgaattgac gcttccagga gcaccggtga cgtcgtggcc
      361 gaagttcttg gcgcttctct ggcgcggcgc ctggatcagg caaccatcga cgagtggatg
      421 agcagagact ttccccgtct gcactcgttc ttccctggct acagactcaa atccgtgccc
      481 gttctgggag atgatctcgg ggcgcgcccg gcatacgatg atccagaggc agtcgccctg
      541 aagggggcct ctcttgatca tcgacagacc gtctgtccga tagtgccttc tcaccgctgg
      601 ctttcacccg aaatagcgct catgatgacc tacttccccg gcaaggcata tgttcagaca
      661 gcaatcagta gttgggatcc gtgtttggcc ttccacgttc agagagacac gttcattgcg
      721 gcgctctcgc tgtttgagct gcagaatatc gcgaacgtcg atggcatgct cacaccgcag
      781 aatagcctgc tgacgacgct ccaactcctc ctggaggacg ccatagctgg tcttgacatc
      841 gaaatcctgc agaacagact gtctcatcat gatacaagtg catttctcca ggaacgcgtg
      901 ccgttgcctc ccgatgctgc atccgccttc tcctcgatcg caacgttcga ctacggagtc
      961 tctctggctc ggttcgtata tcggagtcac ttccgcgaaa tgcgcagagt atcccggacc
     1021 agcaacaaga agatttcgtg ggcatcgttc taccaaagca tattcgaaca gggaaccttc
     1081 gacgtcgctc agctcaggga ttcatgaacg gtgagcctca gtccgggacg gcggacacag
     1141 gccgtccact ctttcttgca atcgccggca acatcggtgt agggaagtcg acgctgacgc
     1201 ggcgcctggc ggacaagtgg gggctcacgc tgctaccgga accggtcaaa cagaacccat
     1261 ttctcaagct ctactacgac gatcccaagc ggtggggatt gcagtcgcag ctcttcttcc
     1321 tcacacagcg agtgaggctc ttcaaggaag cgggagctct tgcctgcgac aagatcgtgg
     1381 accgtacgtt gtatgaggat gcagagatat tcgcccgcac agcgcttccc cacatcgagt
     1441 accaggtcta cgcacaactg tacgagtatc tgctggcaaa cctgcccgta cccgacctgg
     1501 tgatctacct tcgtgcatcg acgaggacgc tgtcgcaacg tatccagtca cgagggcggt
     1561 ccttcgaaaa gtccttgccc tcttcctacc ttcggagact caacgagcgc tatgatgcat
     1621 gggcacgatc gtttctgcgc gctccgctga ttgccattga cactgatgac ctggacctct
     1681 tcagtctctt cggcgaatat gagaaagtga cagacagcat tgcggacgct cttctgcaga
     1741 aaggattgac atggcggcat acgactacga tacccaggcg ttcctgagca tcttccccga
     1801 gactctccag cagtcgatcg aacggcttga cagattcccc gagctcgtcg aggttgtgct
     1861 ggatctcgga cgcgttcccg aagctcggtt cgagttcgac ttcgttcccc tcaacgagaa
     1921 ccacgtcacg agaggggata ttgactccgt cgtccggaag gtgggggaat tcgaccgcga
     1981 caaccgtgcg gggatcgagc ggacgctaca tcgcatttca gccatccgga acaggcacaa
     2041 cgacatcgtc ggactgacgt gccgggtcgg gcgcgccctg tatggcaaga tcgagatcat
     2101 cgaagacctc gtcaagaagg gctcgagcat cctgatcctg ggcagacccg gcataggcaa
     2161 gacaacactc ctgcgcgagg cctcgcggta catcagcgac accctcaaga agcgagtcat
     2221 cattgtcgac acaagcaacg agatcggtgg agacggcgac gtgcctcatc ccggcgttgg
     2281 ctcttcccgc cgcatgcagg tacctctcaa caaggaacaa cacgacatca tgatcgaagc
     2341 tgtggagaac cacaacccgg aggtcatcgt catcgatgag atcggtcgga tcgaagaggc
     2401 acaggcctgc aggacgatcg ctgaacgcgg cgtcgaac
//
LOCUS       DATMZD010000311         6029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_48976,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000311 DATMZD010000000
VERSION     DATMZD010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6029
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48976"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="VN478_06440"
     CDS             <1..666
                     /locus_tag="VN478_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HWQ22347.1"
                     /translation="RYIMEEACKDLGLKFVFETAPDPTSDVGVAGAQQFMLEKTPAWV
                     QKYGKDTAFFCTNDAETEPLLKQLMKYGGMFVEADLPSPLMGYPGALGIDLKAEQGDW
                     PAILKKVEEAVIAQGGSGRFGTWAFSYGYTTTAGLGEYAKRIIDGTAKLGNNADLFAS
                     YEKFTPGVTWNGSNYMDLNTNTASPNHVLVYQDTYVFGKGYLGTTKQVVPEKYLTFKA
                     PGA"
     gene            983..2599
                     /locus_tag="VN478_06445"
     CDS             983..2599
                     /locus_tag="VN478_06445"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HWQ22348.1"
                     /translation="MTEQQPLLQMQGITKEYFGNRVLTDVCLDVNRGEILGLVGENGA
                     GKSTLMNILFGMSVISQTGGYTGKVCIDGREVVFQNPFDALKAGIGMVHQEFSLIPGF
                     SATENILLNGEILHDNIVTKIFGPRMKTLDLPEMRAVADKSIELLGVEIDPDTLVAEM
                     PVGHKQFTELAREIHRPEMKVLVLDEPTAVLTESEAQVLLTSLRRLAATGIAIIFITH
                     RLQEILDVCDRIVILRDGQVVREVATHNTDIGQVAELMVGRSVSSKSAATDVYDEKTE
                     TILSLDHLWVDMPGETVRDVTLEVHKGEIFGIGGLAGQGKLGIPNGVMGLYAAGGSVT
                     LHGKPIRLNDPAAALAAGMAFVSEDRRGVGLLLEEALDWNIAFTAMQSQNKFIRPLLG
                     GFIKWRDDKAIQNVALEYIKALDIKCTGTDQRAQELSGGNQQKICLAKAFAVQPDVLF
                     VSEPTRGIDVGAKALVLEALRTYNRKNGTTIIMVSSELEELRSICDRVAIVDEGRIAG
                     ILPPSASAKEFGLLMIGKLKSEGSEVDSNA"
     gene            2592..3635
                     /locus_tag="VN478_06450"
     CDS             2592..3635
                     /locus_tag="VN478_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWQ22349.1"
                     /translation="MHSIGRFIDRAGWPRIIIGLLLLSLFIAAPSVGVRVDQSITDTI
                     VRFGMNGILVLGMIPMVQTGCGLNFGLPLGIIAGLLGATISIELGLTGIAGFLGAIGL
                     AAPFALLLGWGYGKLLNRVKGDEMMIATYVGFSSVSFMCMMWMLLPYHSPKMIWGYGG
                     SGLRTTISLEGFWMHILDNFLAIRIGRNFMIPTGMILFFVFMALIMWAFFHTKTGTAM
                     TAVGSNPDYARAAGINVDKMRTISVIMSTVYGAIGILVYQQSFGFIQLYNGPFFMALP
                     AVAAILIGGASVNKANITNAVIGTILFQGILTMTPSVINSAIQSDLSEVVRIIVSDGM
                     IVYALTRVTRKSR"
     gene            3640..4749
                     /locus_tag="VN478_06455"
     CDS             3640..4749
                     /locus_tag="VN478_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWQ22350.1"
                     /translation="MTKKTGFKDTISHIFIGNAVPIMFIILIAVAIPLSGFSTGYLIE
                     EMVTRVGRDSFLVLSLLLPIMAGMGLNFGIVLGAMAGQIGIILVTNWGVSGVGGLFLA
                     MAVATPLAVLLGWIAGIVLNRAKGREMVTSLILGFFINGVYQLVVLYIMGKIIPIANP
                     ALVLSRGYGIRNAIDLKGMRQSLDKLIPLKIGGITIPVATLIFIALLCVFIWWFAKTK
                     LGHDMRAVGQDMSVSEDSGIKVERTRIIAIILSTVLACYGQIIFLQNIGTLNTYNSHD
                     QTATFAVAALLIGGASVARASIPNVFVGVILFHLMFVVAPMAGKQLMGSAMIGEYFRV
                     FVSYGIIAVSIVLYAWKRFKERERARRSLRGTSES"
     gene            4913..>6029
                     /locus_tag="VN478_06460"
     CDS             4913..>6029
                     /locus_tag="VN478_06460"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HWQ22351.1"
                     /translation="MGGLIGLDSTWALDRTDPGKIDQAVRNATLQLQILTDRKGAGCD
                     FTGWLDLPVRADESAMTRIEDVATALREGSDTVVVCGIGGSYLGARAGVEFLAGVHPT
                     VGDVPDILFAGNSLSSWQLNKLLDRLRDRRFSVVVISKSGTTLETAVVFHVLRDELIR
                     RFGSEGARTRIVAITDPEHGTLRSLARDEALVAFDMPHDVGGRYSVLTPVGLLPFAIA
                     GLDIRSMLHGAAEQRAACLESPAADNDSLQYAVARRLLGAEGKVIELLTSYEPCLHHV
                     GEWWKQLFGESEGKDGKGIFPASAEFTTDLHSLGQFVQEGTRSLFETVVDVIVPADLT
                     FRCDPGSPDGLAYLDGQSLSGMNHYARIGTVLAHHDGG"
BASE COUNT         1227 a         1772 c         1745 g         1285 t
ORIGIN      
        1 cgctatatca tggaagaggc gtgcaaggac ctcggcctca aattcgtctt cgagactgct
       61 cccgacccca cgagcgacgt cggcgtcgca ggcgcacagc agttcatgct tgagaagact
      121 cccgcctggg tacagaagta cggcaaggac acggcgttct tctgcaccaa cgatgcagag
      181 actgaaccac tcctcaagca gctcatgaag tatggcggca tgttcgtcga agctgacctt
      241 ccgtccccct tgatgggtta cccgggagct cttggcatcg acctcaaggc tgagcagggt
      301 gattggccgg ccatcctcaa gaaggttgaa gaggctgtca tcgctcaggg cggctcgggc
      361 cgctttggca catgggcctt ctcctatggc tacaccacca ctgccggact cggtgagtac
      421 gccaagcgca tcatcgatgg cacagccaag ctgggcaaca atgcagatct gttcgcttcc
      481 tatgagaagt tcacccctgg cgtgacctgg aatggatcca actacatgga tctgaacacc
      541 aatacggcgt cgcccaatca cgtgctcgtg taccaggaca cgtacgtctt tggcaagggc
      601 tacctcggaa cgacgaaaca ggttgttccg gagaagtacc tgacgttcaa ggctccagga
      661 gcctagacca gaagcaacga tcgcgcgccg gggcccacag ccccggcgct ttgtttggag
      721 tatcggatct ggacaggcta tcggcggaaa caaagtctgt gtcgcccagc actagctggt
      781 ggttgtggta caagtgagta tgtctattcg gagcacgttg gttcctgtcg ccttgccgtt
      841 ttccacgtgg cgcagcagac accgttgatc gccgcctgtt gagaccacac cccaaggctc
      901 tgtccgggcc gatgcccacc aggcacatga tggtgttcct gcgtagcgtc tcacggcgat
      961 caatctctga gaggtggtac agatgaccga gcagcagccc ctgcttcaaa tgcagggcat
     1021 cactaaagag tactttggaa acagggtcct gaccgatgta tgtctcgacg tcaatcgtgg
     1081 tgagatcctg ggtcttgtcg gcgagaacgg tgctggcaag tcgacactca tgaacatcct
     1141 gttcgggatg agtgtcattt cccagaccgg aggctacacc ggcaaggttt gtatcgacgg
     1201 cagagaagtc gtctttcaga atccgttcga tgcgctcaag gcagggatcg gcatggtcca
     1261 ccaggagttt tcgctgattc ccggcttcag tgccaccgag aatatcctcc tgaacggcga
     1321 gatccttcat gacaacatcg tcacaaagat ctttgggccg cgtatgaaga ccctggatct
     1381 tcccgaaatg cgcgccgtcg ccgacaagtc catcgagctg ctgggtgtcg agatcgatcc
     1441 tgacactctg gtcgccgaga tgcccgtcgg ccacaaacag ttcacagaac tcgcgcgcga
     1501 aatccatcgc cccgagatga aggtacttgt gctcgatgag cccactgccg ttctgacaga
     1561 gtccgaagca caggtccttc tgacttcgct ccggaggctt gctgccaccg gcatcgccat
     1621 cattttcatc acgcaccgcc tgcaggagat ccttgacgta tgcgaccgga tcgtcatcct
     1681 gcgagacggt caagtcgtaa gagaagtggc cacgcacaac accgatatcg gccaggtcgc
     1741 cgaactcatg gtgggccgca gcgtttccag caagtcggcc gcaacagacg tctacgacga
     1801 gaagaccgag acaatcctct cgcttgatca cctgtgggtc gacatgcccg gcgagaccgt
     1861 cagagatgtg accctcgagg tccacaaggg cgagatcttc ggcatcggcg gtcttgcagg
     1921 tcagggcaag ctgggcatcc caaacggcgt catgggactg tacgctgctg gaggatcggt
     1981 cactctgcac ggcaagccga ttcggctcaa tgatccggcc gccgcccttg ccgcgggaat
     2041 ggcgttcgtt tctgaggatc gtcgaggcgt tggtctgttg ctggaggagg ctctcgactg
     2101 gaacatcgct ttcacggcca tgcagtcgca gaacaagttc atccgcccgc ttcttggagg
     2161 attcatcaag tggcgcgatg acaaggccat ccagaacgta gctctggagt acatcaaggc
     2221 tctggacatc aagtgtacgg gtaccgacca gagggcccag gaactgtcag gtggcaacca
     2281 gcagaagatc tgccttgcca aagcctttgc tgtgcagcca gacgtcctgt ttgtctctga
     2341 gccgacacgc ggcattgatg tcggcgccaa ggcgctcgtc ctggaagccc ttcgcaccta
     2401 caaccgcaag aatggcacga ccatcatcat ggtttcgtcc gagctggaag aactgcggtc
     2461 catttgcgac cgggtggcca ttgtcgatga gggccgtatc gccgggatcc tgccgccatc
     2521 ggcaagtgcc aaggagtttg gcctgctcat gatcgggaaa ctcaagagtg aaggaagcga
     2581 ggtggacagc aatgcatagc atcgggagat tcatcgatag ggccggctgg cctcgcatca
     2641 taatcggcct gcttctgctg tcgctgttca tcgcagctcc atctgtaggc gtgagggtcg
     2701 accagtcgat tacggacacg atcgttcgct tcgggatgaa cggtatcctg gtgctgggga
     2761 tgattcccat ggtccagacc ggctgcgggc tcaacttcgg actacccctt ggcatcatcg
     2821 cagggctgct cggagcgacc atcagcatcg agctcggcct caccggcatc gcgggattcc
     2881 tcggagccat cggcctggcc gccccattcg ccctgctcct cggctggggc tatggcaagc
     2941 tgctcaaccg ggtcaagggc gacgagatga tgatcgcaac gtatgtgggc ttctcgtctg
     3001 tctcgttcat gtgcatgatg tggatgctgc tcccctacca cagcccaaaa atgatctggg
     3061 gctatggtgg gagcggactg cgaactacga tatccctcga gggattctgg atgcacattc
     3121 tcgacaactt cctggccatc agaataggtc gcaacttcat gatcccgacc ggcatgatct
     3181 tgttcttcgt gttcatggct ctgatcatgt gggctttctt ccacacgaag acgggtacgg
     3241 caatgaccgc cgtcggctcg aaccccgact acgcacgtgc tgcaggcatc aacgtggaca
     3301 agatgaggac catatccgtc atcatgtcca cggtctacgg tgccatcggc atcctggttt
     3361 accagcagag tttcgggttc atccaactgt acaacggacc gttcttcatg gcactccccg
     3421 ccgtcgccgc gatcctcatc ggtggagcca gtgtcaacaa ggccaacatc acgaacgcgg
     3481 tgatcggcac gatcctcttc cagggaatcc tcacgatgac accctctgtc atcaacagtg
     3541 ccattcagtc tgatttgtct gaggttgtgc gtatcatcgt ctctgacggt atgatcgttt
     3601 acgccctcac tcgagtgacc aggaagtcca ggtgatacca tgacaaagaa aaccggattc
     3661 aaggacacca tctcgcatat cttcatcggc aacgctgttc cgatcatgtt catcattctg
     3721 atagccgtag cgattccact gtcggggttc tctaccggct atctgatcga ggagatggtg
     3781 acccgggtgg gacgcgattc gttcctcgtc ctctcactgc ttctcccgat catggctggc
     3841 atggggctca acttcggcat cgtgctcgga gcaatggccg gtcagatagg gatcatcctg
     3901 gtcacaaact ggggcgtcag cggcgtaggt ggcctgtttc tggccatggc agtcgccacg
     3961 cctctggcag tcctgctagg ctggattgcc ggcatcgtac tcaaccgcgc caaaggacga
     4021 gagatggtta cttcgctgat cctcgggttc ttcatcaacg gagtgtacca gctggtcgtg
     4081 ctgtacatca tgggcaagat catccccatc gcgaaccccg cccttgttct ctcacgcggc
     4141 tacggaatcc ggaatgccat cgatctcaag ggcatgcgcc agagtcttga caagctcatt
     4201 ccactcaaga ttggcggcat caccattcca gtagccacgc tcatcttcat cgccctgctg
     4261 tgcgtcttca tctggtggtt tgccaagacc aagctcggcc atgacatgcg agctgtcggc
     4321 caggacatga gcgtctccga agactcaggg atcaaggtcg agagaacgcg catcatcgca
     4381 atcatcctgt cgacagtcct ggcgtgctat ggacagatca tcttcctcca gaacatcggc
     4441 actctgaaca cctacaacag tcacgatcag acggcgacgt ttgctgttgc ggcactcctg
     4501 attggcggag ccagtgtcgc cagggcgtcg attcccaacg tgtttgtggg cgtgattctg
     4561 ttccatctca tgttcgtggt cgccccgatg gcaggcaaac agctcatggg ctccgcgatg
     4621 ataggggagt acttccgcgt cttcgtgtca tatggcatca tcgcagtgtc cattgtcctg
     4681 tatgcctgga aacgcttcaa ggaacgagag cgagcgagac gctctttgcg tgggacaagc
     4741 gagtcatagg caggtcaccc tttctccaca ggctctcgaa gacgggctgt cggcatccgc
     4801 cggcagtccg tctcgctctc acggttgctg aaggctccgc agacggtgag ccccgtgctg
     4861 ttgcatggca cccacggtcc cctacaataa tcacgtggac ggaggtgtct aggtgggtgg
     4921 actcatagga cttgacagca cgtgggctct cgatcgcacc gatccgggga agatcgacca
     4981 ggcggtacga aacgcgacgc tccagctcca gatcctgacc gacaggaagg gagccggttg
     5041 cgacttcaca ggatggttgg atcttccggt tcgtgctgac gagagtgcca tgacgagaat
     5101 cgaggacgtc gccaccgcac tccgcgaggg gtccgatacc gtggtggtct gtggcattgg
     5161 cggctcgtac ctgggagccc gggctggcgt cgagttcctt gctggcgtgc accccacggt
     5221 cggggacgtg cccgacatcc tctttgccgg caacagtctc agctcatggc agctcaataa
     5281 gctcctggac agacttcggg accgacgttt ctcggtggtg gtcatctcca agtccgggac
     5341 gacgctcgag actgcagtcg tcttccatgt tctgagagac gaactgatcc gacgatttgg
     5401 ctcggaaggg gcacggacgc gcatcgtggc cattacagac ccggaacatg ggactctgcg
     5461 cagtcttgcc cgagacgagg ccttggtagc gttcgacatg ccacatgacg tcggtgggcg
     5521 ctattcggtg ctcactccgg tcggtcttct tcctttcgcc atcgcaggcc tcgacatccg
     5581 gtccatgttg cacggggccg ctgaacagcg ggccgcctgt cttgagtcac cagcagcaga
     5641 caacgatagc cttcaatacg ccgtcgctcg acgtcttctg ggagcagaag ggaaggtcat
     5701 cgaactgctg acgagctacg agccttgtct gcaccatgtt ggcgaatggt ggaagcagct
     5761 gttcggcgaa agcgagggca aggacggcaa ggggatattc cctgcatcag ccgagttcac
     5821 gacggatctt cactcactcg gacagttcgt ccaggaaggg acgcgctccc tcttcgagac
     5881 agtggtcgac gtcatagttc ccgctgacct cacattccga tgtgatccag gttcgccgga
     5941 cgggcttgcc taccttgacg gacagagtct ctctggtatg aaccactacg cgaggatcgg
     6001 cacggtcctg gctcatcatg acggcgggg
//
LOCUS       DATMZD010000312         5184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_68930,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000312 DATMZD010000000
VERSION     DATMZD010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5184
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68930"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="VN478_06465"
     CDS             <1..722
                     /locus_tag="VN478_06465"
                     /inference="COORDINATES: protein motif:HMM:NF024585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HWQ22352.1"
                     /translation="TDPLVESVRAILPGANCGACGFAGCDAYAIAVAHQTATPDRCTT
                     GGKKAGDQIAALLGLTSTAVDQIAVLVCQGSTDKAPLKAEYTGIQSCRAAKITTGGTK
                     LCQWSCIGLGDCTRVCPFDALHLEDDGLPHVDTVKCTGCGKCVEACPQSVLTRVSRAK
                     KGAIPLCSNRNPVKSRVIATCKTGCIKCEICVKSCPEKAIHMEHGLPVVDEHLCTSCG
                     TCVAKCPTKVFKLIEQDIVRS"
     gene            complement(808..1599)
                     /locus_tag="VN478_06470"
     CDS             complement(808..1599)
                     /locus_tag="VN478_06470"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HWQ22353.1"
                     /translation="MPLAGDLSGFSVDEVISFIMKGGKSGRLVLKSDAIESSLFFTGG
                     AIVEVIHGRVHGPGALASALTAFSTGQFSFYEGENVPVSTMHIDEAQLSGLARQAHIE
                     TETMAPVLPDLDEKLALNVAFADPPSLTPLQWLLLAQIPQRCTLRHLSDGRDPFAVKK
                     ALSPLIVAGLVRRTGQIVSSGIAGVRLTVLKGGTREEEVVEVDQGIVAAWRESGVFAG
                     RVLVAGHVFNALAKHGLGKSIVMTVAACRLCGAHDGQEVDVTPAP"
     gene            1772..5113
                     /locus_tag="VN478_06475"
     CDS             1772..5113
                     /locus_tag="VN478_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22354.1"
                     /translation="MRKRARSVLTGLLVLCCAFASFVPATVVQADSSGSVGRDIIVSE
                     KSGLSRMAYPVSVPLAAESTTVGEWHVTDSAGRPVSCSVRQSESGTPVLQFLTDLPAN
                     SVQTVRLTYSASPSAPGVTQLPLFAGTSYLVYVEKTLEVASYQADNLVRLVRPDGTLI
                     LSRTLGADEPMSCAVASPQVVCVEAAKPVSAWLLTMAPGSEQQALSESNDDVYGAVGT
                     DLLVLTFGQLWLGSPSSTSVSLAAKGSTRTIALEPSSVKFIDGLAPGIYRLRSPSPVC
                     VTSGVEEDNVSCVQYPVGTWFAGPSFGQTTVTSWSHGTTVNASFGGTSRSLLLPELGS
                     SACETTIPLSAFVAGKTEWKPIVLEGDGPFTLTTDGNSGNFGLEQYPSMTLAGAGVSF
                     VITSGKLYSAFSSGHFRRLAVVGVQNGAAVTLTAGSASQHFTLDMAQVKIIRLDTGSL
                     PVRVTSTQPVQVFELGQEAHEGIAVAMPLADSSVTVTVAKVSSAIPAASAGTLAGTQA
                     PALAEQPSSGPWQAAWAWIQDAARATGTWIQSVTRTVGTFFASLYGGIRDFFVSGQAL
                     KTLQTATQAITDLAVRLFLPLSALVVPILARVVSGFSATPELVAVWMFWIIVVVALII
                     LILVLRPRHSEGRPLPKVTPRQAGQGGNTIEFGLVEQGPRPSTATAMDRLTRSDEKQE
                     KPPMAPAVQPAPAEEPSPAHLESPSVPEIALEPLGFFGVAPAVKVDASAVSAPTEAPV
                     LEFTAPETELTTPESTVPEPTTPDAEQAPVVTAEVPSEVLLTSIPESPGVEVPPSEVA
                     REPLPEPELRPMGQESPVTPDEPRRETPDTVLPLDQPVPAEQAPEAGASQIPVPETSV
                     TAQAVPSEEPASIQETNVPVHEEVTAVDETTSTVQETPEIMPEPETLVTETEVVAAVP
                     QPADAFTPVPSSADLLSRLLEEEQSLEQTAVPPSSTTTGAAPTPPSVPVAAPVPRFAP
                     QPSVFSGDLQLDSLVRDGVVVDAGALAQLFRGGYRTQISKLAVSAKDLQNVPEEMRQL
                     VKLTVVALSPIELSIARDLAMRLEAPGYVGEALLVAKKMGYENYLTTYKNISKNYKDV
                     SIVATTSLKVPDVSAESGGDLISFS"
BASE COUNT         1011 a         1648 c         1493 g         1032 t
ORIGIN      
        1 caactgatcc tctcgtcgag agcgtacgtg ccatccttcc gggggccaac tgcggagcct
       61 gcggatttgc tggctgcgac gcttatgcga ttgcagttgc ccaccagacc gcgactcccg
      121 accgctgcac cacgggcggc aagaaggcgg gggaccagat cgctgcgctc ctcggcctca
      181 cgtccactgc ggtcgaccag atcgccgttc tcgtatgcca gggatccacg gacaaggccc
      241 ctctcaaagc cgagtacacg ggcatccagt cctgccgtgc ggccaagatc acgacgggag
      301 gcacgaagct gtgccagtgg tcctgtatcg gccttggaga ctgcaccaga gtatgtccgt
      361 tcgacgccct ccacctcgag gacgacggcc ttccccacgt ggacacggtc aagtgcaccg
      421 gctgtggaaa gtgcgtcgag gcctgtcctc agagcgtcct caccagggtc tctcgtgcca
      481 agaagggagc catccccctg tgctccaaca ggaatcctgt gaagtcgaga gtcatcgcga
      541 catgcaagac gggctgcatc aagtgcgaga tatgcgtcaa gtcctgccct gagaaggcga
      601 tccacatgga acatggtctc cccgtagtgg acgagcatct gtgcacctca tgtggcacct
      661 gtgtcgccaa gtgtccgacg aaggtcttca agcttatcga acaggacatt gtcaggtcct
      721 gactccggac accacatctg ctatcctaca gtagaccccc ggccaattgc agccgggggt
      781 ctggctttcg tgacctgatg ccgtgtctca gggcgcaggc gttacgtcga cctcctgccc
      841 atcgtgcgca ccacacaatc ggcaggcagc aacggtcatg acaatgctct tgcccagtcc
      901 gtgctttgcc agtgcgttga acacgtgccc cgcaacgagc acgcgccccg caaagacacc
      961 ggattcacgc caggccgcca caatcccctg gtcgacttcg acaacttcct cctcccgggt
     1021 gcctcccttg agtactgtca gacggactcc tgcaatgccc gaagacacga tctgtccggt
     1081 tcgccgcacg aggccggcga caatcagtgg cgacaaggcc ttcttgaccg cgaatgggtc
     1141 ccgtccatcg ctaaggtgcc gcaacgtgca gcgctgaggg atctgagcca gaagcagcca
     1201 ttgcagcggc gtcagcgacg gggggtctgc gaatgcaacg ttcagcgcca gcttttcgtc
     1261 gaggtcggga agcaccggcg ccatcgtttc tgtctcgatg tgtgcctgcc gcgcgagacc
     1321 tgacaactga gcctcatcga tgtgcatcgt ggagaccggc acgttctctc cctcgtagaa
     1381 gctgaactgt cccgtggaga atgcggtcag cgcactggca agagcccccg gcccatgaac
     1441 gcgaccgtgg atcacttcga caatcgcccc tccggtgaag aacagagaac tctcgatggc
     1501 atcgcttttg agaacaagcc tccccgactt cccacccttc atgatgaacg agatgacttc
     1561 gtcgacactg aatcccgata gatcgccagc caatggcata cgcgcctcca cgttgacgcc
     1621 ggagtgtgca tcactctttt cggtattggc attatacttg ctgtatcggt ctgcacaagc
     1681 cgactgggaa gaaagtatac ggacagcgcc acgttgttgc aaaacagcaa atggtctgta
     1741 acattcttgc tacaaggatg gtgcgcatag catgagaaaa cgagcgagaa gcgttctgac
     1801 aggacttctg gtactgtgct gcgcctttgc gagcttcgtg cctgcgacgg ttgtccaggc
     1861 ggattcgtcc ggctccgttg gacgcgatat catcgtctcc gagaagagcg gtctctcgcg
     1921 tatggcctac ccagtcagtg tcccgcttgc ggccgaatcg acgacagtcg gcgagtggca
     1981 cgtcacggac tcagcgggaa gacctgtttc ctgttccgtt cggcagtcgg agtccgggac
     2041 tcccgttctt caattcctga ccgacttgcc tgccaattcg gttcagaccg tcaggcttac
     2101 gtacagcgcc agtccttccg ccccaggagt gacccagctg ccactgtttg cggggacatc
     2161 ctacctggtc tacgtcgaga agacccttga ggtagcctcg tatcaggctg acaacctcgt
     2221 gaggcttgtt cgacccgacg ggacactgat cctgtcacgt acacttggcg ccgacgaacc
     2281 gatgtcttgt gcagtagcct caccccaggt agtgtgcgtc gaagccgcaa agcctgtcag
     2341 cgcctggctg ctcacaatgg ctccagggtc agagcaacaa gctctcagcg aatcgaacga
     2401 cgatgtgtat ggtgccgtcg gcactgacct gctggtgctg acctttggtc aactgtggct
     2461 tgggtccccc tcttcaacct ccgtttcgct tgcggcgaag ggaagtaccc gtactattgc
     2521 ccttgagcca tcgtcggtca agttcatcga cgggctggcg cctgggatat atcgtctcag
     2581 gagtccatcc cctgtctgcg tcacttccgg cgtcgaagaa gacaacgtgt cgtgtgtcca
     2641 gtaccctgtc ggaacatggt ttgcgggtcc atcgttcggt cagacgactg tcacttcatg
     2701 gagccacgga accacggtca atgcgtcctt tggggggacg tcgcggtcgc ttctactgcc
     2761 tgaactcggc tcctcggcgt gtgagaccac gattcccctt agtgcgtttg ttgccggcaa
     2821 gacagagtgg aagccaattg tcctggaagg cgatggacca tttacgctga ccacagatgg
     2881 caactcgggc aactttgggc ttgaacagta tccctcgatg actctggcag gtgcaggcgt
     2941 ttcgttcgtg atcacgagcg gcaagctcta cagtgcgttc tccagcggcc acttccgccg
     3001 tcttgctgtc gtcggtgttc agaacggagc tgcagtcaca ctgacagccg ggtcggcatc
     3061 ccagcacttc accctggaca tggcacaggt gaagatcata cgcctcgaca ccggctctct
     3121 gcctgttcgt gtcacatcga cacagcctgt gcaggtcttc gaactcgggc aagaggccca
     3181 cgaggggata gccgtggcaa tgcctctggc agactcctcg gtcactgtga ctgtcgcgaa
     3241 ggtttcgtct gcaattccgg ctgcgtcggc cgggacactc gcagggacgc aggcacctgc
     3301 cctggccgaa cagccctctt caggtccctg gcaagccgcc tgggcatgga tccaggatgc
     3361 agctcgcgca acaggaacat ggatccagag tgtgaccagg acagtgggca cgttcttcgc
     3421 ttccctgtac ggtggcatcc gtgatttctt cgtttccggc caggcattga agacgctcca
     3481 gactgcaaca caggcgatca ctgatcttgc cgtccgcctg ttcctgcctc tctctgctct
     3541 ggtcgtcccg atcctggcac gcgtcgtatc gggtttctcg gcgacccctg agctcgtggc
     3601 cgtgtggatg ttctggatca tcgttgttgt tgccctcatc atcctcatcc tggttctgcg
     3661 tccccggcac agcgagggca ggccactgcc gaaggtgacg ccaaggcaag cggggcaggg
     3721 aggcaacact attgagttcg gtctcgtcga acagggccca agaccgtcga ccgctacagc
     3781 gatggaccgg ctgacgcgca gtgacgagaa gcaggagaag ccccccatgg cgccggccgt
     3841 ccagccggcg ccggccgaag agccatcccc ggcacatctg gaatcgccat ccgtgccgga
     3901 gattgcgctg gagccgcttg gcttcttcgg tgtggcccct gccgtgaagg tggatgcctc
     3961 tgccgtgtcc gcgcccaccg aggcaccggt cctggagttc acagcacccg aaaccgagtt
     4021 gacgacaccc gagtccacag tgccggaacc tacgactccc gatgccgaac aggctcccgt
     4081 cgtcactgct gaggttcctt cggaggtcct tctcacctct atcccggaga gtccaggtgt
     4141 cgaggtgccg ccaagcgaag tggcacgtga gcctctcccc gaacctgaac tgagacctat
     4201 gggccaggag tctccagtca cgcccgatga gccgagaagg gagactcccg acaccgtgtt
     4261 gcccctcgat cagccggttc ctgcagaaca agccccggag gcaggagcca gccagattcc
     4321 tgttccggag acatccgtca ctgcacaggc ggtcccctcg gaagaacccg catcgattca
     4381 ggaaacgaac gttccagtcc atgaggaagt caccgccgtg gacgagacga catcgactgt
     4441 acaggaaacg ccggagatca tgccggaacc tgagacgctc gtcaccgaga ccgaggtcgt
     4501 ggctgctgtt ccgcagccag cagacgcctt cacgcccgta ccttccagcg ctgacctgct
     4561 ctcgaggctc ctcgaggaag aacagtcgct tgaacagact gcggttccac cgagcagcac
     4621 aacaactggg gccgcaccca cacccccatc ggttccggtc gcagctccgg ttcccaggtt
     4681 cgcaccgcag ccgtctgttt tctcaggcga ccttcagctg gacagtctcg tgcgggatgg
     4741 tgtcgtcgtc gacgccgggg cgctcgctca actgttccga ggtggatatc gcacccagat
     4801 ctccaaactc gccgtctcgg ccaaggacct gcagaatgtg cccgaagaga tgcgacagct
     4861 ggtcaagctc accgtcgtcg ctctatctcc gatcgaactg tcgatcgcac gggacctcgc
     4921 catgcgcctc gaggcaccag gatacgtcgg cgaggctctg ctggttgcca agaagatggg
     4981 ctacgagaac tatctcacaa catacaagaa catctcaaag aactacaagg atgtcagtat
     5041 tgtggcgacg acaagtctca aggttccaga tgtgtctgcg gagtccggcg gcgatctgat
     5101 ctcgttcagc taggcctgaa gcgacaccag gaatggagaa gggggcgcaa ttgcgccccc
     5161 ttctccattc ctggtgtcgc ttca
//
LOCUS       DATMZD010000313         6833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_129695,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000313 DATMZD010000000
VERSION     DATMZD010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6833
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129695"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="VN478_06480"
     CDS             <1..510
                     /locus_tag="VN478_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22355.1"
                     /translation="QQGLCRGDRMDDEKLYTLAEAHEEFARQLNGEVWELLDTTDRSS
                     VDEERMLYASWASAYHWLVAGTVVHHQRAEWLLSRVYAVLGDASAAMAHARRCMELTE
                     TTPGEMAPFDLAYACEAIARASALAGDEHEATRYLDLARESGRRIANTEDRQIFEGDL
                     KGGNWYGIS"
     gene            630..1166
                     /locus_tag="VN478_06485"
     CDS             630..1166
                     /locus_tag="VN478_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6448 family protein"
                     /protein_id="HWQ22356.1"
                     /translation="MPPHCDTMDGPVVKAARQALAAGNVKLILPWVPEAAEAELSEAF
                     RKTITVRDKGPDALMLADRWFFETAVRLHRAGEGEPFTGLKPAGLDEGPVIPRAERAI
                     EDGDTAGLIAFLTGAVEEEVGKRFGMIRATRSYDPDDVTAARGYVEAMLDFLVYANKL
                     HVFIRHGGGHGEGVETHH"
     gene            1344..2339
                     /locus_tag="VN478_06490"
     CDS             1344..2339
                     /locus_tag="VN478_06490"
                     /inference="COORDINATES: protein motif:HMM:NF019976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HWQ22357.1"
                     /translation="MAVVIGTSGAWQSVLALAKTAGIVVNEPSDVGIQLARAQKVLSE
                     QDTLARDDLAVQEAALEQKVAERRAAAAQDVARIRARSEQDLAGLNARLENSPSRTPT
                     AATRLWVRMARLSTQAREGAQVRACLRAVLPAERALQDFVADRETEVARRVEGARNVV
                     RGVETLAHSAVLANAIAERAVIRTLATLPDEFVVINDLHLDYDRDLPFENGYLRTAQL
                     DHLLIGPTGIYAIETRNWASTLAADGDESDPILQVARASFLCSRILRDAGCEQIVRSI
                     VACEGAPPTRSGGAHVAVMPTARLLAYAQYGPSLMSFQDVALATSALAEDHRDKL"
     gene            2476..2796
                     /locus_tag="VN478_06495"
     CDS             2476..2796
                     /locus_tag="VN478_06495"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HWQ22358.1"
                     /translation="MMAFARYGGYGRGMMWGGGHILMGLFCLVLFVALILWIIRMVSW
                     RRHGMSCGMHGMYMHGMHDSRDMMQGRPDEALDILRKRFASGDMTKEDYDERLKVLNG
                     EKQP"
     gene            complement(2867..3265)
                     /locus_tag="VN478_06500"
     CDS             complement(2867..3265)
                     /locus_tag="VN478_06500"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWQ22359.1"
                     /translation="MPRPIHFDLGARDPARAIAFYQAAFGWTVKKWDGPFEYYMIMTG
                     DPGEPGIDGGLSPTGNEGILTNLTLGVTLLEDAMAKVTAAGGTIAGEIDTIPGVGRIV
                     NCRDTEGNTFSLIEEIRPDKHVELGEMSGV"
     gene            3548..4399
                     /locus_tag="VN478_06505"
     CDS             3548..4399
                     /locus_tag="VN478_06505"
                     /inference="COORDINATES: protein motif:HMM:NF024102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BsuPI-related putative proteinase inhibitor"
                     /protein_id="HWQ22360.1"
                     /translation="MKRLLIILTILALAVTASVRPVNAADPVAGIRYAPPTTTLATGK
                     LSYAPGEAIDWRYTLYNGNDQPLVLTFTSGQTYDLILRQGSKIVARWSTGKMFADVMS
                     TRTVMMGETMELKGSWQVPAELALGTYDLEFVLTSTSTEPTGAMTQFEVGKATMADVG
                     AKLTTDKLIYRVGTTITVKYTITNLSDKPLVLTFPTSQQYDWTITDGEGNLIYQWMAN
                     KRFAAGVTTLTIPAGTTDGFESSWAIPRDLKPNGFYVHFTVLDDSLGVLKTQRAAVLM
                     GSTPPSQ"
     gene            complement(4470..4676)
                     /locus_tag="VN478_06510"
     CDS             complement(4470..4676)
                     /locus_tag="VN478_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007937382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HWQ22361.1"
                     /translation="MDATNDILMGKWHELKGQVRQQWGKLTDDDLLRLSGKTEELSGI
                     LQQRYGYDKAKAESEINDWLSKHK"
     gene            complement(5412..6620)
                     /locus_tag="VN478_06515"
     CDS             complement(5412..6620)
                     /locus_tag="VN478_06515"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWQ22362.1"
                     /translation="MYRQRWLTERLAQAGRVSRVLVLTGARQTGKSTLLANEPMFSGY
                     HHVTLDDMATLTQAEEEPELLVNMAPNMVIDEAQRAPKLMIAVKKAVDDHPERRFVLS
                     GSANLLLMKHVSESLAGRATYHVLYPPTCSEWRGDAAPRWLLDMFSGRLPEAGTESAP
                     QDLPSTLLTGFMPAIQGASPSDAAYFWEGYVRTYLERDLRELSQVASVVDFRRVMQLA
                     AMRTASLVSQSDIAKDSGVSQPTVWRYLNLLEMSHLYGRLLPAPVTSLKAVTKTPKGY
                     VADTGLAAALAGYQTTASLDETFTGHLLESAVFMALQAISELWMAHLSFFRSGDPPHR
                     EVDFVVERDRKRLAVEVKMSHNVSYADARTIAWLMHEDTQCVGGVVIYAGEDVRVLAQ
                     HIIAVPWTML"
BASE COUNT         1374 a         2121 c         2010 g         1328 t
ORIGIN      
        1 caacaagggc tctgcagagg tgaccgcatg gacgacgaga agttgtacac gcttgccgag
       61 gcacatgagg aattcgccag gcagttgaac ggcgaggtct gggagctgct ggatacgacg
      121 gaccgctcga gcgtcgacga agaacgcatg ttgtacgcct cctgggcttc ggcgtatcac
      181 tggctggttg cagggaccgt cgtccaccac cagcgcgccg agtggctgct ctcgcgcgtc
      241 tatgccgtgc tgggggacgc ctcggcggct atggcgcacg ccaggcgctg catggagctc
      301 actgagacca cccccggcga gatggcaccg ttcgacctcg cgtacgcgtg cgaggctatc
      361 gcccgggcaa gtgctctggc gggcgacgaa cacgaggcga cccgctacct cgaccttgct
      421 cgcgaatccg gcaggcgcat tgccaacaca gaggacagac agatcttcga gggcgacttg
      481 aagggtggca actggtacgg catcagctag gcagaacacc tccagccgtc ctgctctcgt
      541 gcggtcggag caagtgtctg acgggcattg cccggatccg gtggcatcac acgcatcacc
      601 gaccaataca tagcaaagga gatgatccca tgcctcctca ttgcgacacg atggacggac
      661 cggtcgtcaa ggccgcccgc caggctctgg cagcaggcaa cgtcaagctc atcctcccct
      721 gggtccccga ggccgctgaa gcggaactca gcgaagcatt ccggaagacg atcacagtgc
      781 gcgacaaggg gcccgacgcg ctcatgctgg cggatcgctg gttcttcgag accgccgtgc
      841 gcttgcaccg cgcgggtgag ggagagcctt tcaccgggct caaacctgcg ggactcgacg
      901 agggaccagt catcccgcgc gccgagcgag cgatcgaaga cggtgacacc gccgggctga
      961 ttgccttcct gacgggtgcc gtcgaagaag aggtcggaaa gcggttcggt atgattcgcg
     1021 ccacccgctc ctatgacccg gacgacgtca ccgcggcacg cgggtacgtg gaagccatgc
     1081 ttgacttcct ggtctacgcc aacaagcttc acgtcttcat ccgacatggt ggcgggcacg
     1141 gcgaaggagt cgagacccac cactgatacg actggagaac cggccggaga gaccgacctg
     1201 gagcatgggt tgtcaccgat cctgtcggcc ggcgttgtct cggatgatcc gaccagatat
     1261 cgcgggtatg tctcgtcctg cacaccgttg acggagcccc cttcctgtgt ctatactatg
     1321 atggaggact gggaggtgga cgtgtggcgg tagtcatcgg gacaagtgga gcatggcaga
     1381 gcgtgctggc cttggcaaaa acggccggca tcgtcgtcaa cgagccatcc gacgtaggta
     1441 tccagctggc acgcgctcag aaagtcctct ctgagcagga cacgctcgca cgcgacgatt
     1501 tggccgtcca ggaagcagcc ctcgaacaga aggtggccga gaggcgagcc gccgccgccc
     1561 aagacgttgc gcgcatccgc gcccgttcgg aacaggacct cgcaggcttg aacgcccggc
     1621 tcgagaactc cccatcgcga accccgacag ccgcgacacg cctctgggtc aggatggccc
     1681 ggctgagtac gcaagcccgg gaaggtgccc aggtacgtgc atgcctgcgc gccgtcctcc
     1741 ccgccgaacg cgccctccag gactttgtcg ccgatcgcga gactgaagtg gccaggcgtg
     1801 tcgagggcgc ccgcaatgtg gtcaggggcg tcgagacgct ggcccactct gctgtcctgg
     1861 ccaacgccat tgccgaacgc gctgtcatca ggacgcttgc gacgcttccc gacgagttcg
     1921 tggtcatcaa tgacctccac ctcgactacg accgggatct cccgttcgag aacggctacc
     1981 tcaggaccgc gcagctcgac cacctgctga tcgggcccac gggcatctac gccatcgaga
     2041 ccaggaactg ggccagcacg ctcgccgccg acggcgacga atcggacccc atcttgcagg
     2101 tggcgagagc atcctttctc tgctcacgta tcctgagaga tgccgggtgc gagcagatcg
     2161 tgcggtccat cgtcgcctgt gagggcgctc cacccacccg ttccggtgga gcacacgtgg
     2221 cggtcatgcc cacggcgcgg cttctggcct acgctcaata cggtccatca ctgatgtcat
     2281 tccaggacgt tgctctcgcc accagcgcac tcgctgagga ccacagagac aagctgtaga
     2341 cttcccctca gcagcattcg tcttcccatg ttgtcagcca ttcaccttca caatcgcgtg
     2401 tacggaagcc ccggatgctg tgtatagtaa gtacagggac gcagatgcgc cccgtggaaa
     2461 ggagaaatga tacatatgat ggcatttgcg agatacggcg ggtacgggcg cggaatgatg
     2521 tggggcggcg ggcacatcct gatgggcctg ttctgcctgg tgctctttgt tgccctgatc
     2581 ctctggatca tccggatggt gtcgtggaga cgccatggca tgagttgcgg gatgcatggg
     2641 atgtatatgc acggcatgca tgacagtcgc gacatgatgc agggaaggcc ggacgaggcg
     2701 ctggatatcc tcaggaagag attcgccagt ggtgacatga ccaaggaaga ctacgacgaa
     2761 cgactcaagg tcctgaacgg cgagaagcag ccgtaacgag agaaaacgac ccctgtgccg
     2821 caactggcac aggggttttt gcatgtatcg gcgagccctg gacccgtcag acgcccgaca
     2881 tctcgccgag ttcgacgtgc ttgtctggcc ggatctcctc gatgaggctg aacgtgttgc
     2941 cttccgtgtc cctgcagttc acgatccgcc ccacgcccgg gatcgtgtcg atctctccgg
     3001 caatggtccc cccggcagcg gtgaccttgg ccatggcatc ctccagcaga gtgacaccca
     3061 gcgtcaggtt cgtcaggata ccctcgtttc cggtcgggga aagtccgccg tcgattcccg
     3121 gttcgcccgg atctccggtc atgatcatgt agtattcgaa tggtccgtcc cacttcttca
     3181 cggtccagcc gaacgccgct tggtagaatg cgatcgcgcg tgccgggtct cgtgcgccca
     3241 ggtcaaagtg aatcggtcgt ggcatgttat gcctcctgtt tcttgcaggc ctctgcggcc
     3301 tctgcccaaa ccatactccg tgcccaacgc cttgcaaggc cacgcttgac attcacgtca
     3361 caatgcacaa cgatgtagtg cagtggtggc gctcagtcat tgcatgaggt ccctttgggc
     3421 cgcctgagca gccgggacgg gtcggcagac agtcgcctgc gaacgagaac gggaaccggg
     3481 accgtccaag taccgtccaa ttgaagaaga atggtgaccc tcaggggttc gccacatgga
     3541 ggcgaaagtg aagagactct tgatcatcct tacgatactt gcactggcag tcacggcgag
     3601 tgttcggcca gtgaacgctg ccgatccggt cgccggtatc aggtatgcac cgccgacgac
     3661 gactctggcg acgggcaagc tgtcgtatgc gccgggcgaa gcgatcgact ggcgctacac
     3721 gctgtacaac ggcaacgacc agcccctcgt ccttacgttc acgtcaggcc agacgtacga
     3781 cctcatcctg cgacagggca gcaagatcgt tgcccgctgg tcgaccggca agatgtttgc
     3841 cgacgtgatg agcacgcgca ccgtcatgat gggcgagacc atggagctca agggttcgtg
     3901 gcaggtccct gcagaactcg cgctgggcac atatgacctg gagttcgtgc tgaccagcac
     3961 gagtaccgag ccaaccggcg ccatgaccca gtttgaagtt ggcaaggcga ccatggccga
     4021 tgtgggcgcc aagctcacga cagacaagct catctaccgc gtgggaacca ccatcaccgt
     4081 caagtacacg atcacgaacc tgagcgacaa gcctcttgtc ctcaccttcc cgaccagcca
     4141 gcaatatgac tggaccatca ctgacggaga ggggaacctg atttaccagt ggatggccaa
     4201 caagcggttc gcagcgggcg tcacgacgct tacgatcccc gccgggacca ccgacggctt
     4261 cgagagttcc tgggctatcc cgagggacct caagcccaac ggcttctacg tccacttcac
     4321 cgttctagac gacagcctcg gtgtcctcaa gacacagcga gccgccgtcc tcatgggcag
     4381 cacccctccc tcccaataga tgcaacaagc gccccctcac acgagggggc gcttctcttt
     4441 caggtatcct ggttcgtcgt tcccgggacc tacttgtgct tgctcaacca gtcgttgatc
     4501 tcactctccg ccttggcctt gtcatagcca taccgttgct gcaggatgcc ggaaagctcc
     4561 tcggtcttgc cactcagccg caggagatcg tcgtcagtga gtttgcccca ctgctgtcgg
     4621 acctgaccct tgagttcatg ccacttgccc atcaggatgt cgttcgttgc gtccatgtga
     4681 actcctctct ggtctgtcgg gaaacctgcc attagtcgtt taccaactcc ccggacagtc
     4741 cttccggtat gtggcacgtc ctcgtgtctc agcggcatcc cgcacactga ggacagaagg
     4801 agaagaggac aacagacgga tcgactctca aagcaggtca tcatcacgtc ccttctctga
     4861 taagatcagg ggcactgcct gccttgcctg cggaatcctt gacaacgaag ccctggatcg
     4921 acttccccga tggccgcgct gctacaccat ccgttgcctt actctattca gggtgtagca
     4981 ggatcgccca ctcgcgtcaa tgggagatgg tgatggatac tcacaatagt ccctcgcacg
     5041 ttctcaggtt gtgtggcctg ccgttgccgg cacagttgga taccctagtc cgtcatgctt
     5101 ccgagtacgc cgcggccccc cagatctgtc atcgccgagt atgtcggtct ggttcgaggg
     5161 gcgctctcgg cacaggtgaa acccagtctt ccatgcccga ggcccttgat ccgtcattcc
     5221 cccctgagca tttctgtcca atgcgagggg cgttcctggc gcaggcggga atccagttct
     5281 gtattaccgc caagctcgcg gtcaatggct ggtccaggat ggaggcatct attcacgcca
     5341 tgaatagaat ctattcatgg cgtgtgtgat atggcgccta tgttctgtag acatcttcgt
     5401 tccatcttct gctacagcat cgtccatgga accgcgatga tgtgttgcgc gaggactcgg
     5461 acgtcttcgc cagcgtagat gacaacccct ccaacacact gggtgtcctc gtgcatcaac
     5521 catgcgatag tgcgtgcgtc cgcgtaacta acgttgtgac tcatcttgac ttcgaccgcc
     5581 aaccgtttgc gatctctctc gacgacaaag tcgacctcac gatgaggagg atcgccgctc
     5641 cggaagaacg acaagtgagc catccacagt tcggagatgg cttggagcgc catgaacacg
     5701 gcgctttcca gaagatgccc cgtgaaggtc tcgtcaagag acgcagtggt ctggtatccg
     5761 gcaagagcag cggcgaggcc agtgtctgcg acgtatcctt tcggggtctt ggtgacggcc
     5821 ttgaggctgg tcacgggggc aggcaggagc cgcccgtaca agtgtgacat ctcgagcagg
     5881 ttgaggtacc gccagactgt gggttgactg actccgctgt ctttggcaat gtcactctgt
     5941 gacaccagag aagccgtcct catggctgca agttgcatca ctcgccggaa atcgaccaca
     6001 gatgcgactt gcgagagttc gcgaaggtcg cgctccaaat acgtacgaac atagccctcc
     6061 cagaaatacg ccgcatctga cggtgaggct ccctggattg cgggcataaa accggtcaag
     6121 agcgtggacg ggaggtcttg cggtgcactc tcggtaccag cctcaggcag gcgcccgctg
     6181 aacatgtcaa gcaaccaacg gggtgcggca tcccctcgcc actctgaaca cgtaggaggg
     6241 tacagcacat gatacgtagc ccgtccagcc aatgactctg agacatgctt catgagcaga
     6301 agattggcag agccgctcag cacgaaccgg cgctcaggat ggtcatcgac cgccttcttc
     6361 acagcgatca tgagctttgg ggctcgctga gcttcgtcta ttaccatgtt cggagccatg
     6421 ttcacgagca gctccggttc ttcttccgcc tgagtgagcg tagccatatc atcgagcgtc
     6481 acgtgatgat acccactgaa catcggctca ttggccagga gcgtactctt gccggtctgc
     6541 cgggctccgg tcagaaccag gactcgcgaa accctacctg cctgtgcaag ccgttctgtc
     6601 aaccaccgtt gtctgtacat tctattcacc tcgtgaatgt atcctattca ccacatgaat
     6661 agagtatcga tgtccagaca agatacaaca cagtaacctc gggggcgagc aagagccacc
     6721 gaacacaatc tgctagagct tcaggtgtga gaaacgatcc ggttgaactg cccagcgtgc
     6781 tggattactg tggtccgcca ttgtcgccat tttcccagcg cggacctgtc ccg
//
LOCUS       DATMZD010000314         2952 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_100281,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000314 DATMZD010000000
VERSION     DATMZD010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100281"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VN478_06520"
     CDS             <1..180
                     /locus_tag="VN478_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22363.1"
                     /translation="VSDAGVARADFSEGLQQGVGGSTRVLGIRQQIETTVVWISGITS
                     VILSIEGQTEDILQP"
     gene            268..1434
                     /locus_tag="VN478_06525"
     CDS             268..1434
                     /locus_tag="VN478_06525"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HWQ22364.1"
                     /translation="MGRVVYLDNAATTPVDPAVFEEMKPFFTERFANPMTYTHSSSGD
                     TAHVAVEEARSRVASLIGAKPEQVIFTSGGTESDNWVLKGTVERASRERLHRPGHLVT
                     AEFEHHAILHSALALQRQGYEVTFVKVGRSGIVDPDDVRHAMRPNTVLVSIMHANNEI
                     GTIQPIADISRVAHDHGALMHTDAVQTTAHIPVDVNVLGVDFLSVSAHKFNGPKGVGI
                     LFVRDQTALYPFIDGGGQEWGLRGSTHNVPGIVGLGKAAQLGMERLPGELKRLTALRN
                     MLFDKLSTRIDRIAVNGDMKQRVPQNLNIRIEGIDNEPLLLAMNEAGIIAAGGSACNA
                     QETLASHVLTSIGCDLKEAKSSIRLSLGYATTEKDVDYAADVIPGLVRFLRSMA"
     gene            1441..1827
                     /locus_tag="VN478_06530"
     CDS             1441..1827
                     /locus_tag="VN478_06530"
                     /inference="COORDINATES: protein motif:HMM:NF013738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HWQ22365.1"
                     /translation="MDKYSRATIREFMSPSNIGHLDDPSGTGHITDNADAVELTFFIR
                     VDGGIITDAKYRIAGCTALIATLSVLSKELPGRTLEQALAIDMAHLSQAQDGLPESKW
                     RCAGYAVQALQLAIEDYRSKTGSATG"
     gene            1861..>2952
                     /locus_tag="VN478_06535"
     CDS             1861..>2952
                     /locus_tag="VN478_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HWQ22366.1"
                     /translation="MKATVEEVTTRRGLREFVEFPYRLYRGHRGWVPVLRSEMTAIVT
                     GHAGFVLKDNPHAFFVARDDRGRVSGRVGVLVDTRLSAAKGVQYGAFTLFDMVDDWDQ
                     AKALLDAGTSWLRSRGMQVVKGPVSPTNGDDYHGMLAENFEDPPMMYTNYNHPYYNSF
                     VERYGFTVDVRLGAWRFDVRAVDGEREKLVQKVMTRNHYHVDNADMGNLEGTALDIKR
                     ILDEAMPAEWADLTPPSIEDVRIMVRDMKKFVRPEMVAIARTDEGRPIGLVAASPDYN
                     QVFRRMNGRLLPFGWLTFLLYRGRIDAGRALVMFVVPDYRFRGVPAAMFSKLFAWGRT
                     HGYRLAEGSLIGEENVRSWREIEGAGGVRY"
BASE COUNT          607 a          872 c          955 g          518 t
ORIGIN      
        1 gtcagcgacg caggcgtagc tcgcgccgac ttcagtgagg gactccagca gggcgtagga
       61 ggctccacgc gcgtcctggg gatccggcag cagatcgaaa caacagtcgt gtggatatcc
      121 ggcatcacat cggttatact gtccattgaa ggccagacgg aggacatact gcaaccctga
      181 gggtgggcag catgtccgcc gcattcgggc tgtccttgtg tgccacaggc gctgagagga
      241 caggcaacag ggccactgga ggaagcgatg ggaagagtag tctacctgga caatgcagcc
      301 accacgccgg tcgatccggc cgtctttgag gagatgaagc cgttcttcac cgagcgattc
      361 gccaacccga tgacgtacac gcactcatcg tcgggtgata ccgcacacgt ggcggtcgag
      421 gaagcccgct cccgggttgc gtcgctgatc ggcgccaagc cggaacaggt catcttcacg
      481 agtgggggga ccgagtcgga caactgggta ctcaagggaa cggtagaacg ggcgtcgcgt
      541 gagcgactgc acagaccggg acacctggtg acggcagagt tcgagcacca tgccatcctt
      601 cacagcgctc tggctctcca gcggcagggg tacgaggtca cgttcgtcaa ggtcgggcgc
      661 agcggcatcg tggatcccga tgacgtgcga catgccatgc gccccaacac tgtgcttgtc
      721 tcgatcatgc acgcaaacaa cgagatcggc acgattcagc ccatcgccga catctcccgc
      781 gtggctcatg atcacggggc tcttatgcac acggatgccg tccagacgac tgcccacatt
      841 cctgtcgacg tgaacgtcct gggggttgac tttctgagtg tttccgcgca caagttcaat
      901 ggaccgaaag gcgtgggcat cctgttcgtg cgcgatcaaa ccgcgctgta cccatttatc
      961 gatggaggcg gacaagagtg ggggttgcgt ggatcgaccc acaacgtccc cggcatcgtt
     1021 ggactcggca aggcggcgca gctgggaatg gaacggctgc ccggggaact gaagcgtctg
     1081 acggcgctcc ggaacatgct gttcgacaag ctttcgacgc gtatcgacag gatcgccgtg
     1141 aacggcgaca tgaagcagcg tgtgccgcag aacctcaaca tccgtatcga ggggatcgac
     1201 aacgagccgc tgctgcttgc catgaatgag gccggcatca tcgcggcggg cggatcggca
     1261 tgcaatgccc aggagaccct ggcatcgcac gtcctgacgt ccatcggctg tgatctcaaa
     1321 gaggcgaagt cgtcgatacg gctctcgctc gggtacgcga cgacagagaa ggatgtcgac
     1381 tacgctgcag atgtcatccc cggtctggtc aggttcctgc gctcgatggc ctagaggaat
     1441 atggacaagt acagccgtgc cacgatccgt gaattcatga gcccgagcaa catcggacat
     1501 ctcgacgatc cgtcaggtac gggccacatc accgacaatg ccgacgctgt ggaactgacg
     1561 ttcttcatca gagtcgacgg tggcatcatt accgacgcca agtatcgcat cgccggatgc
     1621 accgcactga tcgcgacact atcggtactc agcaaggagc tgcctggcag gacgctggag
     1681 caggcacttg ccatcgacat ggcacatctg tcacaagcgc aggacggcct gcccgaatcc
     1741 aagtggcgct gtgcgggata tgctgtccag gcactgcagc tggcgatcga ggactacagg
     1801 agcaagaccg ggtcggcaac tggctgagcg acgcgcagcc tcttcctcgg acatagaggc
     1861 atgaaggcca cggtagaaga agtcacgacg aggagagggc tccgggaatt cgtcgaattt
     1921 ccataccgtc tctaccgggg gcaccgcggc tgggtgccgg tccttcgttc ggagatgacc
     1981 gcgatcgtca cgggacacgc cggctttgtg ctcaaggaca acccgcacgc cttctttgta
     2041 gcgcgagacg accgcggacg ggtctcaggt cgcgtaggcg tcctggtcga caccaggctc
     2101 agtgcggcca aaggcgtgca atatggagcc ttcaccttgt ttgacatggt cgacgactgg
     2161 gatcaggcca aagcactgct tgacgccgga accagctggc tgcgcagccg aggcatgcag
     2221 gtggtgaagg ggccggtgtc gccgacgaat ggcgacgact accatggcat gctggccgag
     2281 aacttcgagg acccaccgat gatgtacacc aactacaatc acccgtacta caactccttc
     2341 gtggagcgat acgggttcac ggtcgacgtt cgcctggggg cctggcgctt cgacgttcgc
     2401 gcagtggacg gcgagcgcga gaagctggtg cagaaggtca tgacacgcaa ccactaccat
     2461 gtggacaacg cggacatggg caacctcgag gggaccgccc tcgacatcaa gcgtatcctg
     2521 gatgaagcga tgccggcgga gtgggctgac ctgacgcctc cctccatcga ggacgtgcgt
     2581 atcatggtcc gcgacatgaa gaagttcgtg cggcccgaga tggtggcgat cgccaggacc
     2641 gacgagggcc gcccgatcgg acttgtcgcc gcgtctccgg actacaacca ggtcttccga
     2701 cgcatgaacg gtcgtctgct accattcggg tggctgacgt ttctcctgta ccgcggtcgg
     2761 atagacgccg ggcgggctct ggtgatgttc gtcgtccccg actaccgctt cagaggagtc
     2821 ccagcggcaa tgttctcgaa gctgtttgcg tgggggcgca cgcatggcta tcgcctggcc
     2881 gaggggtccc tgatcggcga ggagaacgtg cgcagctggc gggagatcga gggagccggc
     2941 ggcgtccgct ac
//
LOCUS       DATMZD010000315         4032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_100346,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000315 DATMZD010000000
VERSION     DATMZD010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4032
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100346"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1534
                     /locus_tag="VN478_06540"
     CDS             <1..1534
                     /locus_tag="VN478_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22367.1"
                     /translation="YYALWDSTNNKVASAAVVGPDGDVATGTLKNLSSGATATFSTLD
                     SSYKTDNIWVEVDTNTKYVADQWNITVTSYLLSKPDIQRTVKALASRGTPTTAVDSLA
                     DGSWFTHDTVTVTVPGHSFADYAGLYHTKAYFGKYETTSGAIRSDANLYMGGWAKDPV
                     FANNTVYDEAVDDYNGNHDGRFGPDQKSLTWARTHGYSTNGYPAADWANVDDALYGSN
                     MTRNFTDPNGGIQDKAFNEYYVSGNATVVFNANGTVTINGANLPMPPNGAIFVNGTAT
                     VSGTVHGQASVGASKINIGGNIIYTVPPRTDRNAAIPANPDLLGLISHGDITITTATF
                     NANHHLQVDAAMISAAGNFGIDSNAPSHYIDPTGTYAGTWNGCQACWNTSNAPAINLG
                     GNRVRGYEVQHTNFDWNLRDYGVPPFYPTTNSTGESTDLIDQYPVVTNSAIMAVLTGL
                     NKDDLTPTGDSTYPYSYVYNGVTYYYGGTFNWYATSTMIKSALYRISWKEQIASAVTP
                     TP"
     gene            1570..2205
                     /locus_tag="VN478_06545"
     CDS             1570..2205
                     /locus_tag="VN478_06545"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HWQ22368.1"
                     /translation="MRKGFTLIELGVVLVIIMVVSAIAIPSAQSFIATQRLQEVAWQI
                     VQDLKSVKEDAILYQQDLNAYFDYSNSPVEPANAANTNNRSYWFETYQSDPVTKSHYI
                     PTDAATAHFTGRMLKYNIVIDSITAGASSIQIPTGGRNYFVVCFRSGAGSSFRGEGDW
                     VTAMTGRLNSTLSMINTSKVTVKLREPSSNRSYYVIVDGTGKISMYGSPPS"
     gene            2578..>4032
                     /locus_tag="VN478_06550"
     CDS             2578..>4032
                     /locus_tag="VN478_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22369.1"
                     /translation="MRQSIRSIAVVVLCIIVGISGMMPGGSRTCASGASWVERRPAGD
                     VGRDWYSLASDSSGMHLIAAAFGNWGTEGWVYTSTDGGAHWTIRYPSPNSQYWRAAAS
                     DADGSNLIVIAWSGLAYTSSNGGADWTRRYPTGQVVQRLQTVASDADGSNLIVGSSVG
                     RVFASSNGGADWTERRPAGNVDGDWRGASSSADGSVMLIGNYEGRLYRTTDTGTTWSE
                     LVPAGASNQYWQGTACDADGSNLLVGVEEGRLYTSSNGGSSWTERRPVGDTDQNWARF
                     ASDSDGSVLVAGDEARLYLSRDSGATWKEQQPAGDKDVRWTCCSNSDGSYLVAGGYAG
                     GRLYTLGANSGARTLVVAKVGSGTVTLDPAGGTYGDGTVVTLTATPGAGYAFTGWSGD
                     LSGTANPVSVTMDADRAVTATFTALPTYTLTPSAGTGGGITPGTVQNVLRGGSKAFTI
                     SPNVGYQIADVSVDGVSQGRISSYTFMNVTSNHTI"
BASE COUNT          863 a         1158 c         1240 g          771 t
ORIGIN      
        1 gtactacgct ctctgggaca gcacgaacaa caaggtagcc agcgcagcgg ttgtgggtcc
       61 agatggggac gtcgccacgg gcacactgaa gaacctcagt tccggggcga ccgcgacgtt
      121 ctcgaccctc gactccagct acaagacgga caacatctgg gtcgaggtcg acaccaacac
      181 gaagtacgtg gcagaccagt ggaacatcac ggtaacgtcg tacctgctga gcaagccgga
      241 cattcagcgg actgtcaagg ctctggccag caggggaacc ccgaccacgg cagttgattc
      301 tttggccgat ggctcgtggt tcacgcatga tacggtgacc gtcacggttc ccgggcacag
      361 tttcgccgat tatgcggggc tgtaccacac aaaggcctac tttgggaaat atgagactac
      421 gtcaggggcg atcagaagcg atgcgaacct ctacatgggt ggctgggcga aagaccctgt
      481 gtttgcgaac aacacggtct atgacgaggc cgtggacgac tacaatggca accacgacgg
      541 caggtttggg ccggatcaga agagtctgac gtgggccagg acccacggct actccacgaa
      601 cggctacccc gctgcggact gggcgaatgt cgacgatgcg ctgtatggca gcaatatgac
      661 gagaaacttc accgatccga acggaggcat tcaggacaaa gcgttcaatg agtactatgt
      721 cagtggcaat gccacagtgg tgttcaatgc caacggcact gtgacgatca acggcgccaa
      781 ccttcccatg ccccctaatg gggccatatt cgtcaatggc actgccacgg tcagcggcac
      841 ggttcatggc caggccagcg tcggcgcctc caaaatcaac atcggcggaa acatcatcta
      901 tactgttcca ccgagaacgg accggaatgc tgccattccc gccaatcctg atttgctcgg
      961 gctcatctcc catggcgata tcacgatcac cacggccaca ttcaacgcta accaccatct
     1021 gcaggtcgac gcggcgatga tctcggcggc gggcaacttt gggatcgact cgaatgctcc
     1081 ttcccactat attgacccca ccgggacgta cgccgggacg tggaatggct gccaggcgtg
     1141 ctggaacaca agcaacgctc cggcaatcaa cctggggggc aacagggtca ggggatatga
     1201 ggtccagcac acgaacttcg actggaacct gcgcgactac ggtgtgccgc ccttctaccc
     1261 tacgaccaac agtactggtg aatccacaga tctcatcgat cagtatccgg tggtaaccaa
     1321 ctcggcaatc atggcagtgc tgacaggcct gaacaaggac gacctgaccc ccaccggcga
     1381 ctcgacgtat ccttacagct acgtttacaa cggcgtgaca tactactacg gcggcacctt
     1441 caactggtat gcgacttcga caatgatcaa gagcgcgctc tacaggatct catggaagga
     1501 gcagatagcc tctgcggtta cgcccacgcc gtgagccaag gaaaggttcg cgtcctccct
     1561 cgtcctggca tgaggaaggg tttcaccctg atcgagcttg gcgtggtgct ggtcatcatc
     1621 atggtcgtga gtgccattgc cattccctca gcccagagtt tcatcgcgac gcagcgcctg
     1681 caggaagttg cttggcaaat cgtccaggac ctgaagtcgg tcaaggaaga cgcgatcctc
     1741 taccagcagg acctgaacgc gtacttcgac tacagcaact ctcctgtgga accggccaac
     1801 gctgcgaata cgaacaacag gagctactgg ttcgagactt accagtcgga tccggttacc
     1861 aagagccact acatcccgac cgacgcggca acggcgcact tcaccgggag gatgcttaag
     1921 tacaatatcg tgatcgactc gatcacagcg ggcgcgtcgt ccatccagat ccctaccggg
     1981 gggaggaact acttcgtggt ctgtttccgt tcgggagcgg ggagcagttt cagaggcgag
     2041 ggagactggg tgacagccat gaccggacgt ctgaactcca cgttgagcat gatcaacact
     2101 agcaaggtca cggtcaagct ccgggagcca tcttcgaaca ggtcatacta tgtgatcgtc
     2161 gacggcacag ggaagatctc gatgtatggc agccctccgt cctgaggctg gactcgagtg
     2221 tggcgtgccg gagcgccgcg cagtctgaga gccccggttg gtcagcctga cgttgtgacg
     2281 ttcaccgcag cttcggccac cagcagggag aatgctcctg ctggtggctt ctcttgtctg
     2341 tgtccggccc gggagggtga tgcgtcggac cggtcgtgag acatgacgaa cacccgtggc
     2401 ccggtccgac gcgtgggcat gatcgctgtg gcgccgcagg ggtcatggaa catggcaact
     2461 acagcctcga tgcaggcgtg cagacgctgt attggcattc cgagacagtg gaaatgcctc
     2521 acaatggcgg ccacgaccgg ggcaagcagg agacgcagtc cagaggaggc cgagaggatg
     2581 agacagtcaa tccgttccat cgcagtagtt gtcttgtgta tcatcgtggg tatctcggga
     2641 atgatgccgg ggggctccag gacgtgtgcg tctggcgcgt catgggtcga acgtcggccg
     2701 gcaggagatg ttggcagaga ctggtatagc ttggcttctg actcttccgg gatgcacctc
     2761 attgcagctg ccttcggcaa ttggggaaca gagggctggg tctacacctc gaccgatggc
     2821 ggggcgcact ggacgatacg gtatccctcg cccaactccc agtattggag agccgccgca
     2881 tctgacgccg acggaagcaa tctgatcgtt attgcctggt ccggtctggc ctatacgtcc
     2941 tcaaacggcg gcgctgactg gacgagaagg tatccgacag ggcaggtcgt gcagagactg
     3001 cagaccgtgg cgtccgatgc tgatggctcg aacctgatcg tgggatcgtc agtcgggcgg
     3061 gtcttcgcct cttcaaatgg cggtgcagac tggacagagc gacgtcctgc cggaaatgta
     3121 gacggcgatt ggcgcggggc ttcctccagt gctgacggaa gtgtcatgct gattggcaac
     3181 tatgagggaa gactgtacag gactacggat accggaacca cctggtctga acttgttccc
     3241 gcaggggctt cgaaccaata ctggcaggga actgcatgcg acgctgacgg gagcaatctg
     3301 ctcgtcggcg ttgaggaagg gaggctctac acatcttcca acggtggctc cagttggacg
     3361 gaacggaggc cggtgggtga taccgaccag aactgggctc ggtttgcctc tgactcggac
     3421 ggaagcgttc tggtcgcggg agacgaggcc cggctctacc tttcgcggga cagcggcgca
     3481 acgtggaagg aacaacagcc tgcgggcgac aaggacgtgc gctggacgtg ctgttccaat
     3541 tccgatggct cgtatcttgt ggccgggggc tatgcaggtg gcaggctcta cactcttggt
     3601 gccaattctg gtgctcgcac tcttgttgtc gccaaggtgg gcagtggcac ggtaacgctc
     3661 gatccggcgg gtggaacgta cggtgacggt actgtcgtta ccctaactgc cactcccggt
     3721 gccggctacg ccttcaccgg ctggtctggg gacctcagcg gtaccgcgaa ccccgtatcg
     3781 gtcacgatgg atgcagacag ggctgtaacc gcgactttca ctgctcttcc cacctacaca
     3841 ctgacgccat ctgccgggac ggggggtggc atcactccgg gcaccgtgca gaatgtcctc
     3901 aggggcggga gcaaggcctt cacgatctcg ccgaatgtcg gctaccagat tgcggacgtg
     3961 tcagtcgacg gagtgtcgca ggggcgcatt tcctcctaca cgttcatgaa tgtgacgtcg
     4021 aaccatacaa tc
//
LOCUS       DATMZD010000316         5422 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_129838,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000316 DATMZD010000000
VERSION     DATMZD010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5422
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129838"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="VN478_06555"
     CDS             complement(<1..365)
                     /locus_tag="VN478_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013682239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWQ22370.1"
                     /translation="MNPTFGSILIKVLAGAIQSGTILLLPTLGEVLTERSGILNLGLE
                     GLMLIGAFFGFLGAMKTGNPYVGLLLGMAAAAAAAAIHAFICVTLRGNQTVTGLALSI
                     FGAGLTSFYGDSWVSQRLTA"
     gene            complement(362..1579)
                     /locus_tag="VN478_06560"
     CDS             complement(362..1579)
                     /locus_tag="VN478_06560"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWQ22371.1"
                     /translation="MLRFEKREKTPIALRVLVPVLSVLLGLLVGSVVMLFARVNPVRV
                     YGAIVKGAFGSAYTFSETLVIAVPLILISTGLCLVYRMKFFNIGAKGQYIIGAICGSY
                     LALFGPATMSRPLLLTMMLLMGTLGGAVWAVIPALLKVYWNVNEIIATLLLNYIASYI
                     LWFFMYGTWRDPGSHGFPLSKNFALNAQLPRILAAPSRLHIGLFFGLAAALIVWIIIR
                     KTRFGYEARVLGENERAARYAGINVFKTVIIAAVISGGLAGLAGIAHTSAVLRVLQLE
                     INSDYGYTAIIVAWLAGLDPLVAVGVSVLLAALEAGGYQIQIVMRVPFGIVGVIESSI
                     LFCLLAGEIITHYRVTLTRRLGTPTGNTPRIGQKGSGALKNNSETPLALDKRARGRPG
                     QNDARNEGARGAV"
     gene            complement(1598..3124)
                     /locus_tag="VN478_06565"
     CDS             complement(1598..3124)
                     /locus_tag="VN478_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWQ22372.1"
                     /translation="MDGQEELMLVMSDITKRFPGIVANDHVDFALRKGEIHTLLGENG
                     AGKSTLMNVLDGIYYPDEGEITIEGKRVDLRSPLDSMKHGIGMIHQHFMLVDSLSVIE
                     NVILGLESQGFYIDKKTVAGRIEAIAARYSFRVDPYAKIWQLSVGEQQRVEIIKTLFK
                     GARILILDEPTAVLTPQESEELFGILREMRSEGKSIIFISHKLGEVMSVSDRITVLRK
                     GRLVGTVDTASINERELAKMMIGRDVLFRLERKDVELGREILGVEEAQAFNDRGIMAL
                     NKLSIAVHGGEIMGIAGVAGNGQVELAECITGLRHLVGGHVTVEGIDVTNAAPCTLAS
                     AGVNYIPADRLGVGLVPNLSTVENAVLRKYKQQPMCRGAFIDYGAACTYADGLIDKFD
                     IAVPLRSAPVKVLSGGNMQKLLLAREIEENPRLLIAEHPTRGLDVGATEFVRKTLLEQ
                     RDKGVAVLLISEDLDEILMVADRVAVMYEGRIVGIVDAHHVDIAQIGLMMAGAAGARA
                     "
     gene            complement(3186..4307)
                     /locus_tag="VN478_06570"
     CDS             complement(3186..4307)
                     /locus_tag="VN478_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HWQ22373.1"
                     /translation="MKFLRVSLIVLLAIAFVAGSTGCKKAEVTPTPAEKLKVAFVYVG
                     PHNDGGYSQAHEDGRLYLQQNDPNVETTYSESVPEGSQAEKVFRDYAAAGYQLVFGTS
                     FGFMDAMVNTAKDNPTVKFMHASGYKRSDNLGTYFGRMEQPDYLSGLIAGKMTKTNKI
                     GFVLPFSIPECIREVDSFTVGLREVNPKATVTVVYTNSWYDPGKEGDAAKSLLNQNCD
                     IIVSGVDSPAPLDAAFAAGKYGIGYDMDMAAGMKTTEEAKSVLTSRIWHWGPYYLKVA
                     KAVEDGTWTGADYWGGMADGIVDLAPISSAVPQDVIDYVNARKQLILDGKYTPFDGPM
                     VNQKGETVVPAGTTMSDADQLNLQWFVQGVVGDIPKSGS"
     regulatory      4558..4661
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4698..5249
                     /gene="thiT"
                     /locus_tag="VN478_06575"
     CDS             4698..5249
                     /gene="thiT"
                     /locus_tag="VN478_06575"
                     /inference="COORDINATES: protein motif:HMM:NF021062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HWQ22374.1"
                     /translation="MERRSNKAITVKEMAEMGIAVALGVALSYLSKAIWSMPMGGTLE
                     LGVVPVLYISLRWGWRDGLIVGLALGLVTLITDPWFVNPVQIVLDYPLPGLALGLAGL
                     PVFRKGSNWWKGVLTGGLLRYVFHVISGLVFFASAAPSWHMSPLLYSLAYNAAYVVPS
                     IAIAILVLVPLRRVKALWNPGTD"
BASE COUNT         1090 a         1658 c         1560 g         1114 t
ORIGIN      
        1 gccgtgagac gttgagagac ccagctgtcg ccgtagaaac tcgtcagccc agccccgaag
       61 atcgagagcg cgaggcccgt gacggtctgg ttgccccgaa gagtgacgca gatgaacgca
      121 tggatagctg ctgctgccgc tgctgctgcc ataccgagca gcaagccgac gtagggattg
      181 ccggtcttca ttgccccaag gaacccgaaa aatgcgccaa tcagcatcag tccctcaagt
      241 ccgagattca gtatgccgct ccgctcagtc aggacttcac ccagcgtagg aagcagcagg
      301 atcgtcccgg attgaatggc tcccgcgaga accttgatca ggatgctgcc aaaggtaggg
      361 ttcatacggc cccccgagcc ccttcgttcc tcgcatcgtt ctgtccaggg cgcccccgag
      421 cccttttgtc caaggcgagg ggtgtttctg aattgttctt caaggccccc gagcccttct
      481 gtccaatgcg aggggtgttt ccggtgggcg tacccagtct cctcgtcagt gtcacacggt
      541 aatgtgtgat gatctcccct gccagcaggc agaagaggat cgagctctcg atcacaccga
      601 cgatgccaaa gggcacccgc atgacgatct ggatctggta gccgccagcc tcaagagcgg
      661 ccaggagcac agaaacgccg acagcgacca gcgggtcgag cccggccagc cacgccacga
      721 taatcgcggt atatccatag tccgagttga tctcaagctg gaggacacgc agcactgccg
      781 acgtgtgggc aatgccggcc aggccggcga gtcctcccga gatgacggca gcgatgatga
      841 cggtcttgaa cacgttgatg cccgcgtacc tggccgcacg ctcattttca ccaaggaccc
      901 tggcctcata cccgaaacgc gtcttgcgga tgatgatcca gacgatcagg gcggcggcca
      961 gtccgaagaa cagtccgatg tgcagccggg aaggagcagc aagaatccgc ggaagctgag
     1021 cattgagggc gaagttcttg gagagaggaa acccgtggct gcccggatcg cgccatgtcc
     1081 catacatgaa gaaccacaga atgtaagatg cgatgtagtt caacaggagc gtcgcgatga
     1141 tctcgttgac attccagtac accttgagaa gcgcagggat gaccgcccat accgcccctc
     1201 caagggtacc catcaacagc atcattgtca gcagcaacgg ccgcgacatc gtcgcgggtc
     1261 cgaacagcgc cagatatgat ccacagattg ccccgatgat gtactgaccc ttcgccccga
     1321 tgttgaagaa cttcatccgg tagaccaggc agagcccggt ggagatgagg ataaggggga
     1381 ctgcgatgac cagggtctcg gagaacgtgt aagccgatcc aaacgccccc ttcacgatgg
     1441 ctccatagac cctcaccggg ttgacacggg caaacagcat gacgacggac ccgacgagga
     1501 ggccgagcaa aaccgaaaga acgggcacca gtacgcgcag cgctatcggc gtcttttcgc
     1561 gcttctcgaa tcgcagcatc tcaaacccct tttcggatca agcccgggct cccgcggcgc
     1621 ctgccatcat gagtccaatc tgcgcgatgt ccacgtggtg agcgtcgacg ataccgacga
     1681 tgcgcccttc gtacatcacg gcgacccggt ccgcgaccat gagtatctcg tccaggtcct
     1741 cactgatgag cagtacggcg actcctttgt cgcgctgttc cagcagcgtc ttgcgtacga
     1801 actcggtggc ccccacgtcg aggccacggg tggggtgctc agcgatcagg agcctcgggt
     1861 tctcctcgat ctcgcgggca agcagcagtt tctgcatgtt cccgccggag aggaccttga
     1921 ccggggcact cctgagagga acggcgatgt cgaacttgtc gatgagaccg tcggcatagg
     1981 tgcaggcagc accgtagtcg atgaacgccc ccctgcacat cggctgctgc ttgtacttcc
     2041 gcagcacagc gttttccacc gtcgacagat tcggcacgag acccacaccc agtcgatcgg
     2101 ctggtatgta gttcaccccg gctgatgcca gcgtgcacgg agctgcgttg gttacgtcga
     2161 tgccctcgac ggtgacatgc ccgccgacaa ggtggcgcag tcccgtgatg cattccgcca
     2221 actcgacctg cccgttgccc gcgacaccgg caatccccat gatctcgcca ccatgaacag
     2281 cgatgctcag cttgttcagc gccatgattc cacggtcgtt gaacgcctgc gcctcttcga
     2341 cacccaggat ctccctgccc agttcaacgt ccttgcgttc caggcggaac aggacatcgc
     2401 gtccgatcat catcttcgcc agctcgcgtt cattgatgga ggctgtatcg acggtaccca
     2461 cgagccgccc ttttcgcagc accgtgatac ggtcggacac ggacatgacc tcgccgagtt
     2521 tgtggctgat gaagatgata gacttgccct cagatctcat ctcccggagg atgccaaaga
     2581 gctcttcgga ctcctgaggc gtcagcaccg cggtcggctc atccagaatc aggatgcggg
     2641 cccctttgaa cagcgtcttg atgatctcga cccgctgctg ctcgccgacg gacaactgcc
     2701 atatcttggc gtaggggtcc acgcgaaagc tgtacctcgc ggctatcgcc tcgatgcggc
     2761 ctgccacggt cttcttgtct atgtagaaac cctgggattc gagtcccagg atgacattct
     2821 cgatgacaga aagggaatcc acaagcatga agtgctgatg gatcatccca atgccgtgct
     2881 tcatcgaatc aagaggagac cgaaggtcga cgcgcttccc ctctatcgtg atctcgccct
     2941 cgtcgggata gtagatgccg tccaagacgt tcatgagggt cgacttgccc gccccgttct
     3001 cacccagcag agtgtggatc tcgccctttc gcagcgcgaa gtccacgtgg tcgttggcca
     3061 cgatgcctgg aaagcgcttg gtgatgtcgc tcatgacgag catcagttct tcctggccat
     3121 ccatcttttc ctccagagaa gaagaaaggc tccccggcat gacgccgggg agccggtact
     3181 gtcctctatg aaccgctctt tggaatgtcg ccgacgacgc cctgtacgaa ccattgcagg
     3241 ttcagctggt cagcgtcgga catggtcgtg cctgcaggaa ccacggtctc gcccttctgg
     3301 ttgaccatgg gtccgtcgaa cggcgtgtac ttgccgtcaa ggatgagctg cttgcgggcg
     3361 ttcacgtagt cgataacgtc ctggggaaca gccgagctga tgggggccag gtcaacgatg
     3421 ccgtcggcca ttcctcccca gtagtcggcg ccggtccacg ttccatcttc gacagccttg
     3481 gcaaccttca ggtagtaggg accccagtgc cagatgcggg acgtgaggac ggacttggct
     3541 tcctcggtcg tcttcatgcc ggcagccatg tccatgtcgt agccaatgcc gtacttaccg
     3601 gctgcaaaag ccgcatcgag aggcgcagga gagtcgacgc cggatacgat gatgtcgcag
     3661 ttctgattga gcagggactt agccgcgtcg ccttccttgc ctggatcata ccatgagttg
     3721 gtgtatacga ccgtgacggt ggccttgggg ttgacctcac gcaggcccac ggtgaaagag
     3781 tcgacttcgc ggatgcactc ggggatggag aacggcagaa caaatccgat cttgttggtc
     3841 ttggtcatct tgccggcaat cagacccgaa aggtagtcgg gctgctccat gcggccaaag
     3901 taggtaccga ggttgtcgga acgcttgtac ccggacgcat gcatgaactt gacggtaggg
     3961 ttgtccttgg cagtattcac catggcatcc atgaaaccga agctggtgcc gaagacgagc
     4021 tggtagccgg cggccgcata gtcgcggaag accttctcgg cctgcgaacc ttcgggcacg
     4081 ctttccgaat aggtggtctc gacgttcggg tcattctgct gcaggtacag acggccgtct
     4141 tcgtgagcct gtgagtatcc accatcgttg tgcggtccaa catagacaaa ggccactttc
     4201 agcttctcgg caggagtcgg cgtcacttcc gccttcttgc agccggtcga accagcaacg
     4261 aacgcgatgg caagcagtac aatcagtgag actctcagga acttcatact ctctagcctc
     4321 ccatggcaag actgacgttc tgaagcacag ttttccgcac gttccactga ccgggcaccc
     4381 cacttttctc ccgacatgag tctccccgcc cggtactgtc ccattcctct gttcacacgc
     4441 cattgtacca acatcaccct gcaagtaaag tcgggatgca gagatgccgc acgtggctcc
     4501 atgagctgca gggacccatt cgtgactcag acccgttgac tccgggtggt caggcactat
     4561 gctctagtag ggtgcccgaa agggctgaga gagtcatgtg gctcaactcg ttgaacctga
     4621 tctgggtcat gccagcgtag ggagacagag cagtatcacg acaccccgag gtctattcgt
     4681 agattcggga ggtaggtatg gaacgcaggt caaacaaggc gatcacggtc aaggaaatgg
     4741 ccgagatggg aatcgccgtc gcccttggcg tagcactctc atatctttca aaagcgatct
     4801 ggtccatgcc catgggcgga acgcttgagc tcggggtcgt ccccgtcctg tacatctccc
     4861 tcagatgggg atggcgcgac gggctcatcg ttggtcttgc tcttggattg gtgactttga
     4921 tcaccgatcc gtggttcgtc aatcctgtgc agatagtcct cgactatcct ctgccgggac
     4981 tcgcactcgg actggccggc ttgcccgtct ttcgaaaggg ctcgaactgg tggaaagggg
     5041 tcctgacggg aggactcctg cgctacgtct ttcacgtgat atccggcctc gtgttcttcg
     5101 cgtcggctgc tcccagctgg cacatgtctc ctctgctcta ctctcttgcc tacaacgcag
     5161 cgtacgtcgt gccgagcatc gccatcgcga tcctcgtcct ggttccgctc cgccgtgtga
     5221 aggcactctg gaatccggga acagactgat gcatgaccgc aaggggatac tggtcaacct
     5281 gagcctggtc gggctgttcg tcatctgggc gaacgccttc acgttcgtgc gcgttgcgac
     5341 acgatcgctc actcctgcca atctggcgat cctccggttc gccctggtgt ggcctttcct
     5401 gttcctcttt ccgggactgt at
//
LOCUS       DATMZD010000317         2158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_129846,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000317 DATMZD010000000
VERSION     DATMZD010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129846"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="VN478_06580"
     CDS             complement(<1..441)
                     /locus_tag="VN478_06580"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HWQ22375.1"
                     /translation="MTGQTAMISTFGVLMLVAVAIFIWVRRSKFGGWALGLSVAFMLI
                     AAAGLVFSALAGIYTDFLFFSEVGYRQVFWTRTLARVAAYVAGALVAYAVVWAVTGLP
                     HSLFAGMNPEDAQRTSRVLKWTRIIIALVTANFVGLGASGLSLQL"
     gene            complement(715..>2158)
                     /locus_tag="VN478_06585"
     CDS             complement(715..>2158)
                     /locus_tag="VN478_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018963631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HWQ22376.1"
                     /translation="YSPAGAVLRTFGVAGGGRGAGQMYYIHDAVQLSDGLILVSDSEN
                     GRFVAFREDGSVAWTTTRSWYIPGRMAWSRQGTLYVTDGFHNVVRELSYGQPAAAQGA
                     AVVVRLDSVTRLVSAGSTTRLQVSLRNRSAGTDTYSVTSRIRGASGWSSQVNPASVTV
                     LSGATGTTDVTVEVPPGLAPGTVGAVDIDVHSALNPASVTTVHAEVVIRTAPPLVVGG
                     ASIETYMGATVQVPLDARDVEDLYGVGCRVTYDDKALQFVGIAKGSLLGAETLFIEDH
                     GTAGEVLIGSTLEGDALPVSRNGSIAVLTFRALGAGTTQLDIDDLQLAGGEGGRLVLA
                     GKARSIPIRVIGEQPRTVLVLRIGSPTMKSSGSSVVLDAPPVIVEGRTLVPIRAVVES
                     LGGTVAWDAASQTVTISLDGTELKLVIGKSTALVNGKSMPVDSTNPKVVPQILNSRTM
                     LPLRFVAESLGADVQWEVPTQTITITYPKP"
BASE COUNT          414 a          721 c          631 g          392 t
ORIGIN      
        1 gagctgaagg ctgagcccgc ttgcacccag ccccacgaaa ttcgcggtca ccagggcgat
       61 aatgatacgc gtccacttga ggaccctgga cgtgcgctgg gcgtcttccg ggttcatgcc
      121 cgcgaacagg ctgtgtggaa gccctgtgac ggcccacact acggcatatg ccaccagagc
      181 cccagccaca taggcggcga ctcttgccag tgtcctggtc cagaagacct gtctgtatcc
      241 gacttcggag aagaacagga agtccgtgta gatgcctgcc agcgccgaaa acaccaaccc
      301 ggcggccgcg atgagcatga aggccacgct gaggccaaga gcccagccac cgaatttgct
      361 cctgcgcacc cagatgaaga tggcgacggc caccagcatc agcacgccaa acgtcgagat
      421 cattgccgtc tgtccagtca tggtttgtca acctcccatt tcagagcaga gctattgagc
      481 tcatgcaaac caacacgtgt acgcagctgg aggcggtcag gttactgtca ccccattgga
      541 aagcacctgc atcatcctca tgctattggg ggatatggcg ctccttgaga cattctatgc
      601 cgttcgcgcg tcgagacaat gcagcctgcg cctcatctgc atcatgacgc tgcctcttga
      661 atcgagacgc ccccgaagat gggggcgtct tcgtttctgc aacccaaggg agactcaggg
      721 ttttgggtac gtgatggtaa tagtctgggt cggaacttcc cactgcacgt ccgcgcccag
      781 gctctccgcg acaaaacgca gagggagcat cgtgcgactg ttcaggatct gaggcacgac
      841 cttcgggttt gtgctgtcga cgggcatcga cttgccattc accagcgccg tcgacttccc
      901 gatgacaagc ttgagttcag tcccgtcaag cgatatggtc acggtctggc tcgcagcatc
      961 ccaggcgacc gtgcctccaa gcgattccac gaccgcccgg atcgggacca gcgtgcgtcc
     1021 ctcgacaatg accggtggcg catccagcac gacgctggag ccgctggact tcatcgtggg
     1081 cgagccgatg cgaaggacaa gcaccgtcct tggttgctct ccgatgaccc gtatgggaat
     1141 ggaccgggcc ttgcctgcaa gtaccagtct tccaccttct ccaccagcca gctggaggtc
     1201 gtcgatgtcc agctgtgtcg tcccggcacc gagcgcccgg aacgtcagga cggcaatact
     1261 gccgttcctg ctgaccggca gcgcgtcacc ctcaagcgtc gagccgatga ggacctcgcc
     1321 cgcggtgccg tggtcctcga tgaagagcgt ttctgctccg aggagactgc cctttgcgat
     1381 acccacgaac tgcagggcct tgtcgtcata tgtgacacga cacccaactc cgtacaggtc
     1441 ctccacgtca cgtgcgtcca gcggcacctg caccgtcgcc cccatgtatg tctcgatcga
     1501 ggctccccca acaaccagcg gaggagcggt gcggatgacg acctcagcat ggacggtcgt
     1561 gacgcttgcc gggttcagcg cgctgtgaac gtcgatatcc accgccccga ccgtccccgg
     1621 ggcgaggccc ggcgggacct cgactgtcac gtctgtcgta cccgtagctc cggacagcac
     1681 tgtcacagac gcggggttga cctggctcga ccatccgctc gcgccacgga tccgtgaagt
     1741 cacagagtac gtgtcggtcc cggcgctccg gttgcgaagg ctgacctgca gccgcgtcgt
     1801 gcttccagcc gagaccaagc gcgtgacgct gtcgagccgg acgacaacag ccgcgccttg
     1861 cgccgccgcc ggctgtccgt agctcaactc acggaccacg ttgtgaaagc cgtcggtcac
     1921 gtacagggtg ccttgtcgcg accatgccat cctgcctgga atgtaccagg acctcgtcgt
     1981 cgtccaggca accgacccgt cctcgcgaaa cgcgacgaag cgcccgttct cgctgtcgct
     2041 caccaggatg aggccgtcgg acagctgtac tgcatcatgg atatagtaca tctgaccggc
     2101 gccgcggcca ccgcctgcga ccccgaacgt ccggaggacg gctcccgcgg gactgtag
//
LOCUS       DATMZD010000318         2232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_140661,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000318 DATMZD010000000
VERSION     DATMZD010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140661"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="VN478_06590"
     CDS             complement(<1..352)
                     /locus_tag="VN478_06590"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HWQ22377.1"
                     /translation="MKLELQKLNIEEGSSQSFVFEEASVGDLPGITLLEPVTGQFVLS
                     NLGIGYQFSGSFSARVSQPCVRCLDPVVEDVEFDVSETYLLNGQEVPDQEDVFTLESD
                     VFDVSDAVRQNLLIE"
     gene            complement(321..809)
                     /locus_tag="VN478_06595"
     CDS             complement(321..809)
                     /locus_tag="VN478_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22378.1"
                     /translation="MIEKLTLQDIIDRLDQVRERSPRIFGYRIIKDEEFADGIAHLRT
                     AFPEQVRNACALLEQRDALVADARRQCGRMIEEGQRSHDDLVADSEIVRSAIAEREHM
                     MAEIDAARKVAERQADDYSLKMLEQLEQILTKFAETVKASEMQFHVEENSDETRTPET
                     EH"
     gene            complement(802..1311)
                     /gene="coaD"
                     /locus_tag="VN478_06600"
     CDS             complement(802..1311)
                     /gene="coaD"
                     /locus_tag="VN478_06600"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HWQ22379.1"
                     /translation="MRQMDTIVYPGTFDPVTRGHVDIIERGARLCDRLVVGVAAIAYK
                     APAWSLEERVDMVRLATSHLANVSVEGFDDLLVHFMERHGARTMLRGLRPVLDFDYEV
                     QFAWANRHLDPDIDIIYLMSSQEHILVSSTLIKQMYEGGGDIRDLVPASVADRIVLGL
                     SRAKGGTHD"
     gene            complement(1402..2130)
                     /locus_tag="VN478_06605"
     CDS             complement(1402..2130)
                     /locus_tag="VN478_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019413500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HWQ22380.1"
                     /translation="MLVVDELHKSFGSLEVIKGVSFDMDFKERLVLMGPSGSGKSTLV
                     RCINWIEPPSAGRMVFDGKLVEPGKSDIRRLREDIGMVFQQFNVFPHLTALQNVALGP
                     LVVKRVARKVAETEAMELLAKVGLSHRADHKPAQMSGGEQQRVAIARALAMHPKLMLF
                     DEPTSSIDVELIHEVLDVMVRLADEGMTMIVVTHEMGFAKEVADRVIFMDQGLIIETG
                     TPSEVFEHPQHERTQSFLSKVHLA"
BASE COUNT          459 a          711 c          584 g          478 t
ORIGIN      
        1 cttcaatcag caggttctgg cgtacagcgt cggacacgtc aaagacatcc gactcgagcg
       61 taaacacgtc ctcctgatcg gggacttctt gcccattgag aaggtaggtc tcgctgacat
      121 cgaactcaac gtcttcaacg acaggatcca ggcacctgac acaaggctga ctgaccctcg
      181 cgctgaagga cccggagaac tggtacccga taccgagatt gctcagaacg aactgccccg
      241 tcaccggctc aagaagcgtg attcctggga gatctccgac ggaagcttcc tcgaatacga
      301 acgattgact gcttccttcc tcaatgttca gtttctggag ttcgagtttc atcgctgttc
      361 tcctctacat gaaactgcat ctccgacgcc ttcacggtct cagcgaactt tgtcaggatc
      421 tgctcaagct gctccagcat cttcaatgag tagtcatcgg cctgccgttc ggccaccttt
      481 cgcgcggcat cgatctctgc catcatgtgc tctcgctcag cgatggccga gcgcacgatt
      541 tcgctgtccg cgacgagatc gtcgtgtgag cgctggccct cttcgatcat ccggccgcac
      601 tgtcgcctgg catcagctac cagggcatcg cgctgctcaa gcagcgcaca tgcgttgcgg
      661 acctgttcgg ggaacgccgt gcggagatga gcaatgccat ctgcgaactc ctcgtccttg
      721 atgatgcgat atccaaagat ccttgggctc cgctcccgca cctggtcgag ccggtcaatg
      781 atgtcctgca gcgtaagctt ctcaatcatg cgttcctccc ttggcccggg acaggccaag
      841 cacgatcctg tccgccacac tcgcaggcac caggtcacga atgtccccgc ctccctcgta
      901 catctgtttg atgagagtgg aggagacaag gatgtgctcc tgggagctca tgagatagat
      961 gatgtcgatg tcggggtcga gatgcctgtt cgcccacgca aactgtactt catagtcaaa
     1021 gtccaggaca ggccgcaacc cgcgcagcat tgtacgcgct ccatgacgct ccatgaagtg
     1081 aaccagcaag tcatcgaaac cctcgacgct cacgttggcc aagtggctcg tcgcaagtcg
     1141 aaccatgtcg acccgctcct ccagagacca tgcaggcgcc ttgtatgcga tagcggctac
     1201 gcccacgacc agtcggtcgc acagcctcgc tccacgctcg atgatatcga catgtcctct
     1261 ggtgaccggg tcaaacgttc ccgggtagac aattgtatcc atctgtctca tagacctcac
     1321 gttcaagtgt agtcaaaagg cacacgatgg aaagaaaagg aggggcgcct ttcggcgccc
     1381 ctcctcaccg tatcgacaca gtcaggccaa gtgcaccttg ctcaggaaac tctgcgtgcg
     1441 ctcatgctgt ggatgctcga atacctcgga cggggttcct gtctcaatga tgaggccctg
     1501 gtccatgaag atgacccggt ccgcgacctc cttcgcaaaa cccatctcgt gcgtgaccac
     1561 gatcattgtc atgccttcgt ccgccagcct gaccatcacg tcgagaacct cgtggatcag
     1621 ctccacgtcg atggaagacg tcggctcatc aaacagcatg agtttcgggt gcatggcaag
     1681 ggcacgggct atcgccaccc gctgctgctc gccaccactc atctgtgcgg gcttgtggtc
     1741 ggctcggtgg gacaggccca cctttgcgag tagctccatg gcctcggttt cggccacctt
     1801 ccgtgccacc ctcttgacga cgagagggcc cagggcgacg ttctgaagtg ccgtcaggtg
     1861 tggaaacacg ttgaattgct ggaataccat gccgatgtcc tctctgagcc ggcgaatgtc
     1921 tgacttgcct ggctcgacga gctttccgtc aaacaccatg cgcccagcgg acggcggctc
     1981 gatccagttg atgcacctca ccagcgtgct cttgcctgac cccgagggcc ccatcagcac
     2041 taaccgttcc ttgaaatcca tgtcgaagct gacccccttg atgacctcga gagagccaaa
     2101 cgacttgtgc aactcgtcga cgacgagcag cttcccctga gacgtcatca tatcgccggc
     2161 catctcactc caccttcttc tcaagattcc gggaccactg catgaccggg gtcgtcatga
     2221 tcaggtagat ga
//
LOCUS       DATMZD010000319         4623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_89879,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000319 DATMZD010000000
VERSION     DATMZD010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4623
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89879"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="VN478_06610"
     CDS             <1..245
                     /locus_tag="VN478_06610"
                     /inference="COORDINATES: protein motif:HMM:NF015664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HWQ22381.1"
                     /translation="VYLQPAVTGTGIVAGGVVRQIMEKVGVQDVLTKSLGSSNSISLA
                     AATIDGLRDIEQMTLMRNLRRQDRINAEPRATEARQ"
     gene            242..661
                     /gene="rplO"
                     /locus_tag="VN478_06615"
     CDS             242..661
                     /gene="rplO"
                     /locus_tag="VN478_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HWQ22382.1"
                     /translation="MKLNEMVRPEGFIDSPKVIGRGAGSGKGKQSGYGRKGAKARSGR
                     SKRVGFEGGQTPLYRRLPKKKGFRPPVRTVYVAVNIGALEGYEGEMPLDLNALYNAPV
                     KVLGDGELTTKVQVRASGFSASAREKIEKAGGTCEVV"
     gene            661..1926
                     /gene="secY"
                     /locus_tag="VN478_06620"
     CDS             661..1926
                     /gene="secY"
                     /locus_tag="VN478_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HWQ22383.1"
                     /translation="MFETLRRAFRLPELRNRILFTLMMFVVIRLGTYIPVPGIDRAAV
                     ATLVGKGGFLGLLDLFSGGGVANFSIFSLSVLPYINASIIIELLGSVIPALEELRKEG
                     GKEGQRKIAKYTRYLTLGLAVIESFGILAIFGSYLSSTGLFVKLLIVLSLTAGAYIVL
                     WLGEVMTERGIGNGVSLIIFSGIVSRIPVGVAQAFRLTQAKSLSVIGLVGGIIGAIVL
                     LMLVLFAYEGERKVPVQYAKRIVGRKVYGGQSTYIPLKLVQAGVLPIIFASTVLMFPA
                     TVSQFWSTSWFYTKIAQPLTSSGTLAHNIVFFFLIIFFTYFYTEITYDPTKIADDLKK
                     YGGYVPGVRPGEATAQYIKNVLDRIVLPTAIFLAFLAIVPNIAMKNLQISAFAFGGTS
                     LLIIIGVALETVQEIEAYMMMRHYKGFMK"
     gene            1926..2588
                     /locus_tag="VN478_06625"
     CDS             1926..2588
                     /locus_tag="VN478_06625"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HWQ22384.1"
                     /translation="MVQHPRIILLGLPGSGKGTQGALIAQSSGVPTFSSGEYFRSLRR
                     EGKLPAEVAAKISAGGLVSDEDVNMIVVESFLSTQEAQAGFVLDGYPRTVAQAAFLDE
                     YLTKQNVRLTAAVLVDLPVETAFRRISGRRVCPSDGSVYNVYYSQPEREGFCNLCGVP
                     LIQREDDREESVRTRMSTYEEMTKALTVYYGNQGRLVTVDGTGTPDVVFARIREALDD
                     QA"
     gene            2575..3321
                     /gene="map"
                     /locus_tag="VN478_06630"
     CDS             2575..3321
                     /gene="map"
                     /locus_tag="VN478_06630"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HWQ22385.1"
                     /translation="MIRLKSASDIERLRVSGAKLGEVLRQIGEMVAPGVTTHELDRAA
                     ELRIRELGAKPAFLHYSGYPATLCVSINEQVIHGIPGHRILNSGDVVSLDLGLIYEGY
                     YADSAITVACGSIVAQDQELIDATRRSLYAGIDAVHVGARLGDIGHAVQQVAESRGMS
                     VVRDYVGHGVGKELHEDPEVPNYGRAGTGITLKEGLVIAIEPMVNAGSSAVRVLNDDW
                     TVVTVDGHKSAHFEHTIAVTAQGPVILTQP"
     gene            3318..3533
                     /gene="infA"
                     /locus_tag="VN478_06635"
     CDS             3318..3533
                     /gene="infA"
                     /locus_tag="VN478_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HWQ22386.1"
                     /translation="MNKKEVIETEGVIVETLPGTMFRVKLGNGKIVLAHISGKMRLNF
                     IKILPGDKVRLEITPYDLSKARITYRL"
     gene            3564..3680
                     /gene="rpmJ"
                     /locus_tag="VN478_06640"
     CDS             3564..3680
                     /gene="rpmJ"
                     /locus_tag="VN478_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013637834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HWQ22387.1"
                     /translation="MKVRPSVKKICAKCKIVKRNGTLMVICENPKHKQRQGK"
     gene            3691..4065
                     /gene="rpsM"
                     /locus_tag="VN478_06645"
     CDS             3691..4065
                     /gene="rpsM"
                     /locus_tag="VN478_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HWQ22388.1"
                     /translation="MARISGIDLAAGKKVGIALTYIYGIGKSNYKMICSVAGVSEDKK
                     VKDLTESEILAIQKAINDNLVVEGDLRREVSTNIRRLMEINCYRGVRHKRGLPVRGQR
                     TRTNARTRKGPKKTVGSKASKV"
     gene            4080..4475
                     /gene="rpsK"
                     /locus_tag="VN478_06650"
     CDS             4080..4475
                     /gene="rpsK"
                     /locus_tag="VN478_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HWQ22389.1"
                     /translation="MADIKKKVVKRKKEKRVVGEGKAYVFSSFNNTLVTITDAQGDTV
                     CWGSSGSSGFKGSKKGTPFAAQVAAQKAAEKAIAMGMSRVNVFVKGAGSGREIAVRAL
                     QSAGLNVSSIKDVTPIPHNGCRPRKIRRV"
     gene            4485..>4623
                     /locus_tag="VN478_06655"
     CDS             4485..>4623
                     /locus_tag="VN478_06655"
                     /inference="COORDINATES: protein motif:HMM:NF012390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HWQ22390.1"
                     /translation="MSRYIDASCRKCRALNTKLFLKGSRCSSGKCAIERGKMGPGQHG
                     ID"
BASE COUNT         1025 a         1203 c         1400 g          995 t
ORIGIN      
        1 gggtgtatct gcagcccgcc gtcactggta ctggtatcgt cgccggcggc gtagtccgtc
       61 agatcatgga gaaggtcgga gtccaggatg tcctcacgaa gtcgctcggc agctcgaatt
      121 ccatttctct cgccgcggcg acgatcgacg gacttcgtga catagaacag atgacgctca
      181 tgcgcaacct gcgtcgtcag gatcgtatca atgcagaacc gcgcgccacg gaggctcgtc
      241 aatgaagctc aatgagatgg tcagaccaga ggggttcatc gatagcccga aggtcatcgg
      301 tcgcggagcc gggagcggaa agggcaaaca gagcggctat ggccgcaagg gcgccaaggc
      361 ccgttccggg cgaagcaagc gcgtaggatt cgagggtggg cagactcccc tgtatcgtcg
      421 tcttcccaag aagaaggggt ttcggccgcc tgttcgaact gtgtacgttg ccgtcaatat
      481 cggagccctc gagggatatg aaggcgagat gccccttgac ctgaatgcgc tctacaatgc
      541 tccagtgaag gttcttggag acggcgaact gacaacgaag gttcaagtgc gggcgtcggg
      601 cttctcggct tctgctcgtg agaagatcga gaaggctggc ggtacctgcg aggtcgtctg
      661 atgtttgaga cgctgaggag ggctttcagg cttccagagc tgcgcaacag aattctgttc
      721 acactcatga tgttcgtcgt tatcaggctc gggacgtata tccccgtgcc tggtatcgac
      781 cgtgcggcag tggcaacgct ggtcggcaaa ggtggttttc tgggtcttct tgacctgttt
      841 tcgggtggcg gtgtcgcgaa tttctccatc ttctcattga gcgtccttcc ctatatcaac
      901 gcctcgatta tcattgaact cctgggttct gtcatcccgg cgcttgagga gttgcgcaag
      961 gaaggcggca aggaaggtca gcgcaagatt gcgaagtaca cccggtacct taccttgggg
     1021 ctcgccgtca tcgagtcatt cggtatcctg gccatcttcg ggagctacct ttccagcacg
     1081 gggcttttcg tgaagctcct gattgtcttg agtctcaccg ccggcgcata tattgtactg
     1141 tggctcggag aggtcatgac cgagcgaggt attggcaacg gcgtgtcgct gatcatcttc
     1201 tcgggtattg tcagtcgcat cccggtcggc gtagcccagg ccttcaggct gactcaggcg
     1261 aagtcgctga gcgtcatcgg tctggttggt ggcattatcg gggcgattgt actcctcatg
     1321 ctggttctat tcgcctatga gggcgagcgg aaggttccag tgcagtacgc gaaacgtatc
     1381 gtcggtcgca aggtctatgg tggtcagtct acgtacatcc ctctgaagct cgtgcaggcg
     1441 ggggtactcc cgatcatctt cgcgtcgacc gtactgatgt tccccgctac ggtgtcgcag
     1501 ttctggtcaa cgtcctggtt ctacaccaag attgcccagc cgctgacgag ttcgggtacg
     1561 ctggcgcaca acattgtctt cttcttcctc atcatcttct tcacatactt ctacacagag
     1621 atcacctacg atcccaccaa gatcgcggat gacctcaaga aatacggggg ttatgtaccg
     1681 ggcgttcgtc ccggcgaggc taccgcgcag tacatcaaga acgtgcttga tcggattgta
     1741 ctgccgaccg ctatcttcct tgcattcctg gccatcgtgc ctaacatcgc gatgaagaac
     1801 ttgcagatat cggcttttgc cttcggtggc acttcgctgc tcatcatcat tggcgtggca
     1861 ctggagactg tgcaggagat cgaggcatac atgatgatgc gtcactacaa ggggttcatg
     1921 aagtaatggt gcagcacccg cgtatcatct tgttgggcct gccgggttcg ggaaagggca
     1981 ctcagggcgc gctcattgcg cagagcagtg gagtgcccac cttctcgagt ggtgagtatt
     2041 tccgcagtct tcggcgggag ggcaaactgc ctgccgaagt tgcggccaag atcagtgctg
     2101 ggggtcttgt gagcgacgag gatgtcaaca tgattgtcgt cgaatcgttt ctgtcgaccc
     2161 aggaggcgca agctggcttt gttctggacg ggtacccgag gactgtcgct caagccgcat
     2221 ttctcgacga gtatctgaca aagcaaaacg ttcgcttgac cgcagcggtg ctcgttgacc
     2281 tgccagttga gacagcgttc cgtcgcattt cggggcggcg cgtctgccca tccgatggca
     2341 gtgtatacaa cgtgtactat tcgcagccag agagagaggg cttctgcaat ctctgtggcg
     2401 tgcccctcat ccagcgggaa gacgaccgcg aggagagtgt caggacaagg atgagtacct
     2461 acgaagagat gacgaaggcc ctgactgtct actacggcaa tcaggggaga ctcgtcactg
     2521 ttgatgggac tggaactcca gatgttgtct ttgccagaat acgggaggct cttgatgatc
     2581 aggcttaagt ctgcatcaga catcgagcgt cttcgtgttt ccggagccaa gctcggcgag
     2641 gttttgcgcc agataggaga gatggttgcc cccggcgtga ctactcacga gctggaccgt
     2701 gcggcagaac ttcgcatccg cgagctcgga gcgaaaccgg cattcctgca ttacagcggc
     2761 tacccggcaa cactctgtgt ctccatcaac gagcaggtca tccatggcat tccggggcat
     2821 cgcattctga attcaggtga cgtcgtttcg ctggatctgg gcctcatcta tgaaggatac
     2881 tacgcagact cggccattac ggtcgcctgc ggatccatcg tggctcagga ccaggagctt
     2941 attgacgcga cccgaaggag cctgtacgcc ggcatcgacg ccgtgcatgt gggagctcgt
     3001 ctgggagaca ttggccatgc agtccaacag gtggccgaat cccggggcat gagtgtcgtg
     3061 cgtgactacg tagggcatgg tgttggaaag gaactgcacg aggatcctga agttcccaac
     3121 tacggcagag ccggtacggg tattactctc aaggagggac tggtgatcgc gatcgaacca
     3181 atggtcaacg ctggcagcag cgctgtccgc gttctgaacg atgattggac ggttgtcacc
     3241 gtggacgggc acaagagcgc tcacttcgag cacacgattg ccgtcacggc ccagggcccc
     3301 gtcatcctta cgcagccatg aacaagaaag aagtcataga gacagagggt gtcatagtgg
     3361 aaacgctccc agggacgatg ttccgcgtga agctgggcaa cggcaagatc gtgctggccc
     3421 atatcagcgg aaagatgagg ctcaacttca tcaagatcct tccgggcgac aaggtaaggc
     3481 ttgagatcac accctatgac ctttcgaaag cacgtatcac ctatcgactg taactgtcga
     3541 agagccaaaa ggagtagcga cctatgaaag tgagaccatc tgtaaagaag atctgtgcca
     3601 agtgcaagat agtgaagcgc aacggtacgc tcatggtgat ctgcgagaac ccgaaacaca
     3661 agcagcgcca aggtaagtaa aggaggaata gtggctcgta ttagtggtat cgatcttgct
     3721 gccgggaaga aggtaggcat tgccctcacc tacatctatg gcatcggcaa gtcaaactat
     3781 aagatgatct gcagtgttgc aggagtgtcc gaggacaaga aggtcaaaga cctgacagag
     3841 agcgagattc ttgcgatcca gaaggcaatc aatgacaatc tcgtcgtcga gggtgacctt
     3901 cgtcgggaag tctcgacgaa catccgtaga ctcatggaga tcaactgcta ccgtggtgtc
     3961 cgtcacaaga gaggactacc cgttcgcggt caacgcacca ggacgaatgc ccgcacgcgc
     4021 aagggaccga agaagactgt cggttccaag gcatccaagg tataggcaaa ggggatctta
     4081 tggcagacat caagaagaaa gtagtcaagc gcaagaaaga gaagcgggtt gttggcgaag
     4141 ggaaagcata cgtcttctct tcattcaaca acacccttgt aacgattacg gacgcccaag
     4201 gcgacactgt gtgctggggt tcgtctggaa gcagcggatt caagggttcc aagaaaggaa
     4261 cgccgttcgc tgcccaggtt gccgcacaga aagcggccga gaaggctatt gccatgggga
     4321 tgtccagagt gaacgtgttt gtgaagggcg ctggttcggg tcgcgagatc gctgtccgtg
     4381 ctctgcagtc tgccggcctc aacgtttcgt ccatcaagga cgtcacgccc atcccgcaca
     4441 acgggtgcag gccgcgcaag atcagaaggg tataggaggc gaccatgtcc agatacattg
     4501 atgcgtcgtg ccggaaatgc cgtgcactga atacgaagct gttcctcaag ggatctcggt
     4561 gttcgagcgg caagtgtgcg atcgaacgag ggaagatggg accgggacag catggaatag
     4621 acc
//
LOCUS       DATMZD010000320         3149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_59810,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000320 DATMZD010000000
VERSION     DATMZD010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59810"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VN478_06660"
     CDS             complement(<1..258)
                     /locus_tag="VN478_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012882650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWQ22391.1"
                     /translation="MAVHSGMRGMINAPDEKPKITRQLLRRVLTYAKPYRWMIVGMLI
                     LILANTGLMLLTPLVLRDLIDHTIPSGNIGRLMMLAAALLLI"
     gene            449..>3149
                     /locus_tag="VN478_06665"
     CDS             449..>3149
                     /locus_tag="VN478_06665"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HWQ22392.1"
                     /translation="MDERTPRLRRTAVLVLLLLAVSITTSCKRKTPTNLGQTGSTPYG
                     TAVTWGPLKLSRGYVGDFRVEGDIGVGSVVYDSGASAEGTYLYALDTSSGNLIGDASL
                     PGSIYATDIGERYVFIWVRDGISVYDLKKQFAFVQTIPTGAPRWVMNLGDFLYVEVRD
                     APDYLIDKRTLKTRDLHISDDDFIYKLLYPDCVIEDPSLDPTGQGDPAVYSLETDKVL
                     GHIPSKYLAGEIFAWEPQNNLFFCTDRDKLVVYDFSKGQIVSEIMLTSSETYAIVADS
                     HSSPAHYFGNGDRLHVTGNATSLTDDSYFFNGKKYAYVVDTSGRVIAEQAGSEILGRF
                     GGYAFSRKSDSISPSQSGGDALWTKKGPEKPFHGIGPYFADSSIVWLCDSSRRQDHGD
                     GIYQWDYKTGSFLNYTEFTGYEAQVLSSQPLIVAMRNEKGGVSAGWYLVCYKLSKLPF
                     NWTPDFSIDCSPDKVYSSATDTTFTCAIKSVPAEFGLNDISYAWNFGDGTTGTGNGIV
                     HRFAKDGNYNVTVSVRFGNSVDALSKSLQIAVLKSPDIRLVATPEYYVADGLTYRLEY
                     KSDNLSAFGNVAWDFGDGNKGSTTNVLHTYKLGNYTAKATVTNTDKTQSWHAEVAISA
                     RYPEFTVSASTSAGDTALEVNFSCSLASTDLTDVGLSYTWKTGSDVIGTRSTLDKVFV
                     DAGTYSTTLEVRDPASRLVTSKTFQTTVYGPHLVLSDGGRRLESSCPIFIEPEYDVDK
                     DGINQAWEDEAMRTVNPYFELDEEEDWFRYQDTDKVANYVRVAPYTNKSNEKYILFFY
                     AICWSRDYGRYSVDANPIVEVKGLEAHDGDVEKVVQAWKVIDDSNLELQYVYTSAHGT
                     EATSHSSVWSASGQSCNTGKTLLWPSKKTVEDRMCATLEFNNDI"
BASE COUNT          737 a          840 c          888 g          684 t
ORIGIN      
        1 gatcagcaga agcgctgcgg cgagcatcat caacctgccg atattcccgg atgggatcgt
       61 atggtcgatc aagtcgcgca agacgagtgg cgtcagcagc atcagacccg tatttgccag
      121 gatcagtatc aacatgccga cgatcatcca gcgatagggt ttggcgtatg tgagcacccg
      181 cctgagcagt tgccgcgtga tcttcggctt ctcatcaggt gcattgatca tgcctcgcat
      241 gccgctgtgg actgccatcg tgtcgcctca ccgatccaga ttccctcttc tgatgattat
      301 cgcatgcttt ggcggggtgt ccagcgcacg aggaacgttc tctccacgcg gaaggggagg
      361 actcctggtg gttcgattgc aaattgccat gtttgttgga ttattggtgc aggaacagtc
      421 ttgaggggga gtgacgagtg gtatgctcat ggacgagaga actccacgtc tgagacgaac
      481 tgcggtcctt gtcttgctgc ttcttgcagt gagtatcact acctcgtgca agaggaagac
      541 gccaaccaac ctgggtcaga caggctcgac gccctatggc actgcagtaa cctggggacc
      601 actcaagctg tccaggggat acgtgggaga tttccgcgtt gagggcgaca ttggagttgg
      661 ctctgtcgta tatgactcag gtgcttctgc tgaaggcaca tacctgtatg cgcttgacac
      721 atccagcgga aacctcatcg gagatgcgag tctccctggc agcatctatg cgacggacat
      781 tggtgaaagg tatgtcttca tctgggtaag ggacggaatc agcgtctacg acctgaagaa
      841 gcagtttgcc ttcgtgcaga cgattccaac gggagctcct cgatgggtga tgaaccttgg
      901 cgacttcctg tacgtcgagg ttcgggacgc ccccgactat ctgatcgaca agcggacgct
      961 gaagacgagg gacctgcaca tatcggacga tgacttcatc tacaagctcc tctacccgga
     1021 ctgcgtcatt gaagacccat ctcttgaccc taccggacag ggagatcctg cggtctacag
     1081 cctggagacg gacaaggtcc taggacatat acccagcaag taccttgcgg gcgagatctt
     1141 cgcctgggag ccacagaaca acctgttctt ctgcactgac agagacaagc ttgtcgtcta
     1201 tgacttctcg aagggccaga ttgtctctga gatcatgctc acgagttctg agacctatgc
     1261 catcgttgcg gactcccata gcagccccgc gcactatttt ggcaatggag accgtctcca
     1321 cgtcacaggt aatgccactt ctctgaccga cgattcctac ttcttcaatg ggaagaagta
     1381 tgcgtatgtc gtggacacgt ccggcagggt catcgccgag caagcaggtt cagagattct
     1441 cggaaggttt ggaggctacg cgttttccag gaagtctgat tccatttctc caagtcagag
     1501 tggtggcgac gccttgtgga ccaagaaagg gccggagaag ccgtttcatg gcataggtcc
     1561 atatttcgcg gatagctcta ttgtgtggct gtgtgattcg agtcgccgac aggatcatgg
     1621 tgacgggatc taccagtggg actacaagac aggctccttc ctcaactaca ctgaattcac
     1681 gggctacgaa gcccaggttc tctcttctca accactgatt gttgctatgc gcaatgagaa
     1741 gggaggcgtc agcgcgggct ggtatctcgt gtgctacaag ctgtcgaagc tcccgttcaa
     1801 ctggacgccc gacttctcca tagactgcag tccagacaag gtctactctt cggcaacgga
     1861 cacaacgttc acctgcgcga tcaagagtgt gccggccgaa ttcgggctga acgacatctc
     1921 gtatgcttgg aactttggag atggcacaac ggggactggc aatggaattg tccaccgctt
     1981 cgcaaaggac ggcaactaca atgtgactgt atccgtgaga ttcggcaatt ctgttgatgc
     2041 tctcagcaaa tcgttgcaga ttgcagtgtt gaagtcacct gatatcagac tcgtcgccac
     2101 gccggagtac tacgttgcag acggccttac gtacaggctt gagtacaaga gcgacaatct
     2161 cagcgcattc ggaaacgttg cgtgggattt cggggatggg aacaaaggca gcacgacgaa
     2221 tgtgctgcac acgtacaagc ttgggaacta cacagcgaag gctaccgtta ccaatacgga
     2281 caagacccag tcatggcatg cagaggtggc aatcagtgcg aggtacccgg agttcacggt
     2341 gagcgcttcc acttctgcag gagacactgc actcgaagtg aacttcagct gcagcctcgc
     2401 aagcacggat ctcacagacg tgggtctctc ttacacgtgg aagacaggat cagacgtcat
     2461 cggtaccaga agcactctgg acaaggtctt cgtcgatgcc gggacctact cgacgactct
     2521 tgaagtgaga gaccctgcat ccagacttgt caccagcaag acgtttcaga ccacggtcta
     2581 cggtccccac cttgtcctct ctgatggcgg aaggagactc gagagcagtt gcccgatctt
     2641 catcgagccc gagtacgacg tcgacaagga cggcatcaac caggcatggg aagatgaggc
     2701 aatgcgcacg gtcaacccct actttgagct ggacgaggaa gaggactggt tccgctacca
     2761 ggacactgat aaggtggcca actacgtgcg ggtcgcgccg tacacgaaca agtcaaacga
     2821 gaagtacatc ctcttcttct acgccatttg ctggagcagg gactatggac ggtacagcgt
     2881 ggatgccaat cccatcgttg aagtcaaagg attggaagct catgacgggg acgttgagaa
     2941 ggtcgtccag gcatggaaag tcatcgatga cagcaacctc gaactgcagt acgtctatac
     3001 ttctgctcat gggaccgaag ccaccagcca ctcgtccgtg tggagcgctt cgggacagag
     3061 ctgcaacacg ggcaagaccc tgttgtggcc ttccaagaag accgtagagg accgcatgtg
     3121 tgcgaccctc gagttcaaca acgatatcc
//
LOCUS       DATMZD010000321         2948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_9807,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000321 DATMZD010000000
VERSION     DATMZD010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9807"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            540..1217
                     /locus_tag="VN478_06670"
     CDS             540..1217
                     /locus_tag="VN478_06670"
                     /inference="COORDINATES: protein motif:HMM:NF016121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HWQ22393.1"
                     /translation="MTRRIVSFVIAAALVVVAFTGCAKKATWTDGTYEGFSKVDDYGN
                     LGHATVTIAGDKITAATYVELTPKTKDNYSYPTAVDAVATMQAKLIETGDIAQVDAVA
                     KATGTSEQTTEAVNNALKDAGTVGSYTDGTYSGFSTADDRGNIGYASVTVSGGKITDA
                     KLLALQVKTADNYKYPTSIAAWPTLIQSLVEKQDVTTVDAVSQATQTTDRFKETVTNA
                     LDKAKAQ"
     gene            1388..2044
                     /locus_tag="VN478_06675"
     CDS             1388..2044
                     /locus_tag="VN478_06675"
                     /inference="COORDINATES: protein motif:HMM:NF017747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutD family protein"
                     /protein_id="HWQ22394.1"
                     /translation="MTARYPQYVVELVRRDRQHVSTWSGGRTTEVAIYPRGAAYGHGG
                     FLWRISSASVDSVEAEFTFLPGIWRLIMVLEGDMTLEHERRHSVRLRPFQQDAFAGDW
                     VTRSKGRARDFNIMLATGCAGWMQAATVQGQSTCAMQTGNDSECGEGGHVTVIVYAVD
                     GSVVADFGIEEEYRVDIGDALLLTTDGHRAPCSVQLSSQGPDPVHVVLAAIRYDEVQR
                     "
     gene            2239..>2948
                     /locus_tag="VN478_06680"
     CDS             2239..>2948
                     /locus_tag="VN478_06680"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HWQ22395.1"
                     /translation="MHRDEFRYRTRDGLNLFARTWAPDGEEKGVVALVHGLGEYGGRY
                     VDIGQSFVDRGYVFVAGDLRGHGVSEGRRAFAARLDNFMDDLDQYCDEIRGRFPGKPL
                     FLYCFSMGSTLGAAYLVRRHPRLAGAILVSGGFLMPPASAAAMNKVRALRLFVPTMAV
                     RNGMGPNRSKVCHDTGVLEAYDADPLVYKKVTVGLAAIIGEADAEDLARAAEIQVPLL
                     VMHGEDDIIALPEGSKRLA"
BASE COUNT          581 a          841 c          942 g          584 t
ORIGIN      
        1 cgtccacccg tcttgttccg gaaacggatt tccatctact gacgtcatgt gtgcgggcag
       61 ccccgaggag cgagtctcct cgaggcaggt cgatgccctg gcatagagaa gtgcgagctg
      121 ctgatgtacg agtcgagcta cgtcgtccat gacagcctcc tgggttacta ccagcagtat
      181 acgagcgctc cagggcggca caaggaatag cgtccctgaa cccggtgtaa cgtgacggcg
      241 tgggaggcga ttctgtaggt gccgggtcct gccctcgtcc acgaggccca ggctcggctg
      301 ccttcctcag gggcggggtc gcgaccgccc cttgatcatg tcgcagggcg gcctccggag
      361 tattctgcgc aatgcgaacc aactgtgaac tcccagttgt gttgcgcaag aggaacgagc
      421 gcgcttgctc cccacgatgt caaccacaag acatcacaat tctgattgac ggaggtcctg
      481 gcgcacggta caatacatac gttactaatc tctccgaatg ggaaggagta ctgcacaata
      541 tgaccagaag aattgtctcg tttgtcattg ctgccgctct cgtcgtcgtc gctttcaccg
      601 gttgcgccaa gaaggccacc tggacggacg gaacgtacga gggcttctca aaagtggacg
      661 actacggtaa ccttgggcat gccacggtga cgatcgccgg cgacaagatc acggcggcga
      721 catatgtcga gcttacgcca aagaccaagg acaactactc atatccgact gctgtcgatg
      781 cagttgcgac catgcaggcc aagctcatcg agaccggcga cattgcacag gtcgacgccg
      841 ttgccaaggc gaccggtacg tccgaacaga cgacggaagc tgtcaacaac gccctgaagg
      901 atgccgggac tgtcggttcc tacacagatg gcacctatag cggtttctct acggcagacg
      961 accgcggcaa catcgggtac gcttccgtca ccgtttcggg cggcaagatc actgatgcca
     1021 agcttctggc cctgcaggtg aagacggcgg acaactacaa gtacccgacg agcattgctg
     1081 catggccgac tctcatccag agcctggtcg agaagcagga cgtcaccacg gttgatgctg
     1141 tctctcaggc cactcagacg accgatcgtt tcaaggagac tgtcactaac gcattggaca
     1201 aggccaaggc tcagtagcag catcactcta cttgccttgt catgccggcc ccatatgggg
     1261 ccggcattct cattgcgtga cccgtgaccg ggctgttggc atggcagcgt gacggtcgtg
     1321 atatactgaa tggcgaagtg gtcatcctgc ctgcagcgat ccatgcgagt gagaggagaa
     1381 gtacgttgtg acggcacggt atcctcaata cgtggttgaa ctggttcggc gcgatcgaca
     1441 gcatgtcagc acgtggagtg gcggcaggac gaccgaggtc gccatctatc ccagaggtgc
     1501 cgcctacggc catggtggct tcctctggcg catcagctcg gcttctgtgg actcagtgga
     1561 agccgaattc accttcctgc ccgggatctg gaggctgatc atggttcttg agggggacat
     1621 gactctggag catgagcgtc gccacagcgt gcgattgcgg ccgtttcaac aggatgcgtt
     1681 tgccggcgat tgggtcacca ggagcaaggg aagggcaagg gacttcaaca tcatgctggc
     1741 gacaggctgc gctggctgga tgcaggccgc cacggtgcaa gggcagtcga cgtgtgctat
     1801 gcagactggg aacgactcgg agtgcggcga gggcgggcat gtcaccgtga tcgtgtatgc
     1861 ggttgacggg tcagttgtcg cggacttcgg gatcgaggag gagtaccggg tcgacatcgg
     1921 ggatgcgctg cttctcacga ccgatggcca ccgggcacca tgcagcgtgc agctgtccag
     1981 tcagggccct gacccggtcc acgtcgtgct ggcagcaatt cggtatgacg aagtccaacg
     2041 gtaggattcg agcactcatt cgtccttctc tctcgagagt ccttgtcaat gcgaggagtg
     2101 ctgtcagtgc atgagggatc ccatcccggt cttgccgtat gggtcatggc gcgcagagtc
     2161 cttcatgttc gcccggagag gtcaccctgg gagtatacta tcagcgtccg gcaatgacgc
     2221 cgactttggg aggctgcgat gcacagagat gagttccggt accgtaccag ggatggtctg
     2281 aacctctttg cccggacctg ggcgcccgac ggagaggaga agggtgtcgt tgccctggtt
     2341 cacgggttgg gcgagtacgg tgggcggtat gtggatatcg gacagagctt cgtggaccgg
     2401 gggtacgtgt ttgtcgccgg agacctgcgc ggacacggcg tctccgaggg caggcgggcg
     2461 tttgccgcgc gtctggacaa cttcatggat gacctcgacc agtactgcga tgagatccgg
     2521 gggcggtttc ccggaaagcc gctgtttctg tactgcttca gcatgggttc gaccctgggt
     2581 gccgcatacc tggtccgcag gcaccccagg ctggcgggcg cgatcctcgt cagcggcgga
     2641 ttcctgatgc cgcccgcttc tgcggcagcc atgaacaagg tgagagcact ccggctcttc
     2701 gtaccaacga tggctgtccg gaacgggatg ggtccgaacc gcagcaaggt ctgtcacgat
     2761 accggcgtcc tcgaggcgta cgatgccgac ccgctcgtct acaagaaggt caccgtcggc
     2821 ctggcagcca tcatcggcga agcggacgcg gaagaccttg cccgtgcagc tgagatccag
     2881 gttcctctgc tcgtcatgca tggggaggac gacatcattg cgctgcccga agggtcaaag
     2941 cgtcttgc
//
LOCUS       DATMZD010000322         4137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_140759,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000322 DATMZD010000000
VERSION     DATMZD010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4137
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140759"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..1050)
                     /locus_tag="VN478_06685"
     CDS             complement(133..1050)
                     /locus_tag="VN478_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014733424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase family
                     protein"
                     /protein_id="HWQ22396.1"
                     /translation="MFSIIVHGGVGAVPSDKQEAVIAGVREAVVAGSNVLASSGSSLD
                     AVETAIRVLEDNPLFNSGTGSVLTFDGEVEMDAAVAYGPALGFGAIAGIKNIQHPISL
                     ARMVMEKTDHVLLQGSGAQEFARMMGIAPHNPVTEERRVQWREYREKFLRGDAGDWPK
                     LKALMKEHPEFMHGTVGAVAVDEKGEVTAGTSTGGVFLKLLGRVGDTPLPGAGTYATT
                     FGGASSTGLGESMMRTLITKTACDFMRMGLDAQGAASGAVNMLSNILGTEAGIIVVDN
                     QGGVGFAQNSSQMAHAWFKKGMSEPVAGL"
     gene            1172..1801
                     /locus_tag="VN478_06690"
     CDS             1172..1801
                     /locus_tag="VN478_06690"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HWQ22397.1"
                     /translation="METATKLVEIARRLYDRNMNAALGGNVSIRLGEEVAITPAGINK
                     GFMTEDDVVVVDMNGTRLRGNGKPSSEGKMHYEIYKLRPDVQAVIHAHPPFAVGFALA
                     HQDLPDDILPEATIVLGHVPCLPYVTPSTIELAREVAQGLAHRNAAFMANHGAITVGA
                     DLEEAYNRMELLEQTCMSVVYASILGGVHHIPEPEMEFFTTNFGLKREG"
     gene            1846..3327
                     /locus_tag="VN478_06695"
     CDS             1846..3327
                     /locus_tag="VN478_06695"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HWQ22398.1"
                     /translation="MSVAHGTGAIELHELQSILQERSHRKAVQRAVEAIARLVPSCSV
                     SLLTIGPRGAPRFEAWANVPASVVRGAERSFSREGLPRNLLDVVRKKKSVVIEDLSSY
                     ADWREPVPPTASWAGFPIVLHGRVIAIVNVQTLKRRITPAMIDVIEPVARTIALLILR
                     YEEAHELAARSRQQRILYEMAMLGMRQQEMGEMLGQVLQLVGRILRNPYVDILLYDAR
                     REVLVLTANRGHQVRRIGTELDARSSKGVTVRAFQTRKPVIVRDTKTSREFVEGLWPA
                     RSELAIPLVVQGGCVGVLNLESLKVGAFAQSDIRLLSPFTSGLSLLIDNQQKAKQLKE
                     QATRDGLTGFFNHRMMDEMVPRELQRAARYHRDVSLAMLDLDNFKAVNDHLGHREGDR
                     LLRVFAACIRKVVRTSDFMYRYGGDEFLILLPETTIGQAEEILSRLDAVLCPELATAL
                     GPVTFSAGIASYVSDPSAEDLVKLADDRLYQSKRLGKGQITSH"
     gene            3414..3875
                     /locus_tag="VN478_06700"
     CDS             3414..3875
                     /locus_tag="VN478_06700"
                     /inference="COORDINATES: protein motif:HMM:NF014629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HWQ22399.1"
                     /translation="MKIMICAQGGGLKKSYNKMAQDEYCRRLEHYCEVSVVDVASRQL
                     ANYARFEEGARVLLDSRGQEITSRELSERLQDLMNMGTRKLSFFIGGAEGFNPTLQTY
                     IEQNVGVRPLSPGTSILSFGKLTLPHELARVILLEQLYRAFSILKGEPYDK"
BASE COUNT          805 a         1298 c         1243 g          791 t
ORIGIN      
        1 atccatagtg tagcgggata gcactgcgcg ccaagttcac gtgacagtca ttgtcgtgcc
       61 tgtgggaatc caatcctgga atgagagcgg ggggagcgtg agctcccccc gctggttcag
      121 gtacgtgacc gtctacagcc ccgcaacagg ttccgacatc cccttcttga accaggcatg
      181 agccatctgg gacgagttct gggcgaagcc gacgccgccc tgattgtcga cgacgataat
      241 ccccgcctcg gtccccagga tgttactcag catgttgacc gcgcctgaag cggcgccctg
      301 ggcgtcgagc cccatgcgca tgaagtcgca ggctgtcttg gtgatcagcg tgcgcatcat
      361 gctttcgccc agtccggtcg acgatgcgcc gccgaacgtc gtggcatagg tgccggctcc
      421 gggcagcggc gtgtcgccga cccgccccag cagtttgaga aagacgcctc ccgtcgacgt
      481 tcccgcggtc acctcgccct tctcgtcgac ggcgacggca ccgacggtgc cgtgcatgaa
      541 ctccgggtgt tccttcatca gggccttcag tttcggccag tccccggcgt cgcccctgag
      601 gaacttctcc cggtactctc gccactggac gcgccgttcc tccgtgacag gattgtgagg
      661 tgcgatgccc atcatacggg cgaattcctg ggcgccgctc ccctgcagga gcacgtggtc
      721 cgtcttctcc atgaccatgc gtgccagcga gatggggtgc tggatgttct tgatccccgc
      781 gatggctcca aatccaagcg cgggaccata cgcgacagca gcgtccatct cgacctctcc
      841 gtcgaacgtc aacactgacc ccgtaccgga gttgaagagg ggattgtcct ccaggaccct
      901 tatggcagtt tccacagcgt ccagcgagga tccgctgctc gcaagaacgt tactcccggc
      961 aaccacggct tcccggaccc cggcaatgac agcttcctgc ttgtcactgg ggaccgcgcc
     1021 gacgccgcca tgaacgatga tcgagaacat agcgcctccg tactcgacag gcttacttac
     1081 cttccaagta tatggtatac tcattgattg gcaacttggc ttcagaagcg gcgaagaggc
     1141 cttgaggccg gagaggtacg cggaggcaac gatggagact gcaacgaagt tggtagagat
     1201 agcaaggaga ctgtacgacc gcaacatgaa cgccgccctg ggtggcaacg tgagcatacg
     1261 gttgggcgaa gaggtcgcca tcaccccggc cggtatcaac aagggattca tgaccgaaga
     1321 tgacgtcgtt gtggtggaca tgaacggaac caggcttcgg ggaaacggta agccgtcgtc
     1381 cgaaggcaag atgcactatg agatctacaa actccgtccc gatgtccagg ccgtcatcca
     1441 cgcgcacccg ccgtttgcag tcgggtttgc gctggcgcac caggacctgc cggacgacat
     1501 cctgccggag gcgacgatcg tgctgggcca cgtcccgtgc ctgccgtacg tcacgccgtc
     1561 gacgatcgag ctggcccgtg aggttgccca gggacttgca caccgcaacg ctgcgttcat
     1621 ggcgaaccat ggcgcgatca cggtcggcgc ggacctggag gaggcataca accgcatgga
     1681 gctccttgag cagacatgca tgtccgtcgt gtatgcgagc atcctcggcg gcgtgcacca
     1741 catccctgag ccggagatgg agttctttac caccaatttc gggctgaagc gcgagggctg
     1801 agccagcctg gaaaactctc atccagttgg taaaatggtg gtgaaatgag cgtggcacac
     1861 ggcaccggcg ccattgaact gcacgaattg cagagcatcc tgcaggaacg ttcacaccgg
     1921 aaagcggtgc agcgcgccgt cgaggcgatt gcgcggctcg taccatcgtg cagtgtgagt
     1981 ctcctgacga tcggcccacg gggtgcgccg cggtttgagg cctgggcgaa cgtccccgca
     2041 tccgtcgttc gcggagcaga acgcagcttc agccgtgagg gtttgcccag gaatctgctc
     2101 gacgtggtgc gcaagaagaa gtccgtcgtc atcgaggacc tcagctcgta tgctgactgg
     2161 cgcgagccgg tgcccccaac cgcttcctgg gccggctttc ccatcgtcct tcacggccgc
     2221 gtcatcgcca tcgtcaacgt ccagaccctg aagcggcgta tcactccggc aatgatcgac
     2281 gtcatcgaac cagtggcgag gaccatcgcg ctcctcattt tgcggtacga ggaggctcat
     2341 gaactggcag cgcgcagtcg ccagcagagg atcctgtacg agatggcgat gctgggcatg
     2401 cggcagcagg agatgggcga aatgctcggg caggtcctgc agcttgtcgg cagaatcctg
     2461 cgcaacccat atgtggacat cctgctatac gatgcgcggc gcgaagtgct cgtcctgacg
     2521 gccaaccggg ggcatcaggt ccggcgcatc ggcacggagc tggacgcgcg gtcgtcaaag
     2581 ggtgtaacgg tgcgtgcgtt ccagactcga aaacccgtca tcgtccgcga tacgaagaca
     2641 tccagggagt tcgtcgaggg cttgtggccg gcccggtctg aactcgccat cccgcttgtc
     2701 gtgcagggag gatgtgtcgg agtgctcaac ctcgaatcac tcaaggtcgg ggcattcgcg
     2761 cagtccgaca tccggttgct ctcgccgttt acttccgggt tgtcccttct catcgacaac
     2821 cagcagaagg cgaagcaact gaaggaacag gcgacgcgcg acggactgac cgggttcttc
     2881 aaccatcgca tgatggacga aatggtccca cgggagctgc agcgtgcggc tcgctaccat
     2941 cgcgatgtga gcctggccat gctcgacctc gacaacttca aggctgtcaa cgaccacctg
     3001 ggccacaggg aaggggaccg cctgcttcgc gtcttcgcgg cttgcatccg caaggtcgtc
     3061 cgcacctcgg acttcatgta ccgctacggc ggagatgagt tcctgatcct gttgcctgag
     3121 acgaccatcg gacaggccga ggagatcctg agtcggctgg atgcggttct gtgccccgag
     3181 ctcgccacgg cgcttggtcc tgtcacgttc tcggccggga tcgcgagcta tgtcagtgat
     3241 ccctccgcag aggacctcgt caagctggct gacgaccgcc tgtaccagtc caaacggctt
     3301 ggcaagggac agatcacgag tcactagcgt gcacatgcct gtcccatgca tcatttgagc
     3361 tcgtacggga gtccgatcct tgtccggccg gctgcagccg ctatactgac tgcatgaaga
     3421 tcatgatatg cgcccagggt ggcggtctca agaagtccta caacaagatg gcgcaggacg
     3481 agtactgccg tcgcctggag cactactgcg aggtcagcgt cgtcgacgtc gcctcgcgcc
     3541 agctcgccaa ctatgcacgg ttcgaagaag gcgcgcgcgt cctgctcgat tcccgcggtc
     3601 aggagatcac gtcacgcgaa ctgtccgagc gcctgcagga cctcatgaac atgggtacac
     3661 gcaaactgtc gttcttcatc ggcggcgccg aggggttcaa cccgacgctg cagacatata
     3721 tcgaacagaa cgtcggggtc cgtcccctct cgcctggcac gagcatcctg tcattcggga
     3781 agctgaccct gccgcacgag ctggcacgcg tcatccttct cgaacagctc tatcgggcgt
     3841 tctcgatcct caaaggggag ccatacgaca agtagcctgc cctcaactgt gatgacacct
     3901 gtcacaatct gcgtttgaga cttatccaca ccctgcaacc gtttgcatca ggaccggaag
     3961 aactcgtcaa tactgcgtct gcagacaact gccactcttc cgtgccaacc tgctgacagg
     4021 ttatccacaa gctcagacgt cgcttttgtg cggctcagcc gtcatcttcc cacgtttcta
     4081 cccactttcc acaggtctgt attcacagac atggctggtg atgcgggccg tggcggc
//
LOCUS       DATMZD010000323         2422 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_140792,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000323 DATMZD010000000
VERSION     DATMZD010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140792"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            247..843
                     /locus_tag="VN478_06705"
     CDS             247..843
                     /locus_tag="VN478_06705"
                     /inference="COORDINATES: protein motif:HMM:NF036410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5317 family protein"
                     /protein_id="HWQ22400.1"
                     /translation="MLVLVVLVCALCLSLATGGRLRYVEDFRLMALPLGVGAFVAQLL
                     IFTSRGKALLGGLLPGAYMVTLLMLLAFLLVNRRVFGVPILLAGLVLNIMVIGANLGR
                     MPADPQALIATGQLGHAEELIRNGTAANVVLMSGETRLNLLGDYIVLPILGDMGSAYS
                     PGDLVALVGEAVLVYGMVRKKRSQRVDPAGDKPAGSDS"
     gene            927..2033
                     /locus_tag="VN478_06710"
     CDS             927..2033
                     /locus_tag="VN478_06710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HWQ22401.1"
                     /translation="MHIRELYDLVDTVAPFRLQEEYDNAGLIVGSMDDDVRGVLLCLD
                     VTHETVEEAGRVGANIILSHHPPVFRAIRKLDSVQHSALLAAIRMDIALMAAHTNFDA
                     APDGLNAYVARRMGLTDLQVLDVHETERSYKVVTFVPPEFRGQVLEAMFAAGAGHTGS
                     YSHTSFRASGTGGFVPDPGTHPFIGEEGAMTSVEEDRVETIVSGRDLASVVKALCAAH
                     PYEEPAVDVFEEHVPGRPSAGFGHLGTLPHAMGPGEFVMFIKSFFGLSTLPVAGTLPE
                     AIERVAVCTGSGSSVLAAATGSGAQVLITGDVTYHTALDASQCGNCVVIVDHYSSERF
                     FAAAMEQALQTAARDRELPPLYRSTADYQPVRFW"
BASE COUNT          445 a          680 c          777 g          520 t
ORIGIN      
        1 tgtcggttcc gcgtacagtg ttggtgacct ggtcgcgctg gcaggtgagg ctgtgatcgt
       61 gtttggcgtg gtgagacaga aacaggcaag ccatcgatcg aaagagacga tgtgatcggt
      121 ctgcttgcgg tgtagcaaag gaaactgttc agagaatcca tcatagcgaa agaactgggg
      181 tgagaaaatg gctggtcaat acgcgtcttc tgcgagtcac gctgtcgtgg cgcaggcagg
      241 ctgacagtgc ttgtgcttgt cgttcttgtg tgcgctctgt gtctgagtct ggctaccggc
      301 gggcgactgc gctacgttga ggattttcgg ctcatggcac ttcccctcgg agtcggggcc
      361 ttcgtggccc agctcctcat cttcacgtct cgtggcaaag cgctgcttgg gggacttctt
      421 ccgggagcct acatggtgac actgctgatg ctgctggcgt ttctgctggt gaaccgcaga
      481 gtcttcggtg tcccgatcct cctcgcaggt ctcgtgctca acatcatggt catcggggct
      541 aacctcggac gcatgccggc tgacccgcag gccctcattg cgaccggaca gctgggccac
      601 gcagaggaac tgatccgcaa cggcaccgca gccaacgtcg tcctcatgag cggcgagaca
      661 cgcctcaacc ttctggggga ctacattgtc ctgcccatcc tcggcgacat gggctcggca
      721 tacagtccgg gcgatctcgt ggctcttgtg ggagaagccg ttctcgtgta tggaatggtg
      781 cggaagaaga ggtcgcaacg ggtggatcca gccggtgaca agcctgccgg aagcgacagc
      841 tagtccgttc ggcaggatcg cctgtcggtt cagcctccct cgcgtatact gttggcgaaa
      901 gcagcgtgct gcacgggagg tgctggatgc atatccggga actgtatgac ctcgtcgata
      961 ctgtggctcc gtttcggttg caggaggagt atgacaacgc aggattaatc gtcggttcga
     1021 tggatgacga tgtgcgcggt gtcctgctgt gtctcgacgt cacgcatgag accgtcgagg
     1081 aggccggccg ggtcggcgcc aacatcatcc tgtcccatca cccccctgtc tttcgtgcca
     1141 tcaggaagct ggactctgtc cagcactcgg cgctcctggc tgccattcga atggacatag
     1201 cgctcatggc ggcacacacg aacttcgacg ctgccccgga cggcctcaac gcatacgtgg
     1261 cacgccgaat gggactcact gacctgcagg tgcttgacgt ccacgaaacg gaacggtcct
     1321 acaaggttgt cacgttcgtt cccccggagt tccgtgggca ggtcctggag gcgatgttcg
     1381 ccgcgggggc ggggcacacc ggctcctatt cgcacacatc tttcagggct tcggggacgg
     1441 gcgggttcgt gcccgaccct gggactcacc cgttcattgg cgaggagggc gccatgacca
     1501 gcgtggaaga ggaccgcgtg gagaccatcg tctccggccg tgaccttgca tccgtcgtga
     1561 aggctctgtg tgcagcccac ccatacgagg agccggcggt cgacgtcttc gaagagcacg
     1621 tgccggggag gccgtcggcg gggttcgggc acctcgggac tctgccccat gcgatggggc
     1681 cgggagagtt cgtaatgttc atcaagtcct tctttgggct gtccacgctt ccggtggcgg
     1741 gcactctgcc ggaggctatc gaacgtgtag cagtctgcac cgggtcggga tcgtcagttc
     1801 ttgccgctgc gactgggtct ggagcccagg tgctgatcac gggagatgtc acatatcata
     1861 cggcgctcga cgcgtctcag tgcggaaact gcgttgtcat cgtggaccac tacagttctg
     1921 aacgcttctt cgcggctgcg atggaacaag cgttgcagac tgctgcgcgg gacagggagt
     1981 tgccaccgtt gtacaggtcg accgccgact atcagccggt caggttctgg taggaagggc
     2041 ggcaaagaaa acagcccgaa gcgcgattgc ggtccgggcc gttttcgaag gagggcatgt
     2101 aaccgataac aaagttaccg attacaccgc cactataggg gatatgttct tcttgtcaac
     2161 ggcaacacat gcacgcatct gcattggctg gaagtatgcc tgcagaccgc gtatcagttg
     2221 gcgtcacagt gttgaacggc ctccgaatcg tgctattgtt ctgtcatgaa tacagaggac
     2281 aacgtttcga tcgagcagtt tggacgttcg acctggctgg agatcaacct ggcctccata
     2341 cgtggcaact accaggtcgt tcgggggttg gtgcccgcga aaacacatgt gctgtgcgtc
     2401 gtcaaggacg atgcctatgg ac
//
LOCUS       DATMZD010000324         4240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_130223,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000324 DATMZD010000000
VERSION     DATMZD010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4240
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130223"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            367..1104
                     /locus_tag="VN478_06715"
     CDS             367..1104
                     /locus_tag="VN478_06715"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HWQ22402.1"
                     /translation="MPFVATAVLFGLVVLATHFIQGITGFGCAVLAMPFCILLTGIDT
                     ARPVLTVLALVLDVYLLAVSWKHVNWRQYLRILAFVLPGLPVGMVAYNILPETTLMHI
                     LAVFMVVVGIQGLVTQFRPNRGAPRPWPAWLLDSLLFIGGAMQGAFTSGGPPVIIYAT
                     QKLKDKASFRATLVAIWTTLNVFIITDIVVRHKLDGVPGKLVLTSLPFLALGALLGDI
                     AHRHISGERFTRLIYAVLLISAVFMVL"
     gene            1149..2222
                     /locus_tag="VN478_06720"
     CDS             1149..2222
                     /locus_tag="VN478_06720"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HWQ22403.1"
                     /translation="MIAFTRGVPATEAFPVEALGECASSALAKYSSQILQYGSFRGFG
                     PLREFIAAGHGLVPDQVLLSQGALQIVDFAARSLITPGTTVLVEQPSYDRVIKVLRRA
                     GATVVGVPLEADGMDLAACEDLIREHHPLMAYVISDFQNPSGTTMSETRRRAFAALAD
                     RYNLLILEDVPYRRLRYAGEDVPTIRSIVPQRTIQLTSFSKLLSPGLRVGVAFGPQPY
                     MDRLGRMAQDTYITPALLPQATVFEFIDRGLLPAQIEKLHGLYRPRLSRLLGALDAGM
                     SDLATWARPEGGFFVGVTLKKDVKAEALIARGLEEGIQLTNGRDFFADGRGDTFVRLP
                     FCALTTEEIDEGVRTLAWVVRSL"
     gene            2248..3063
                     /locus_tag="VN478_06725"
     CDS             2248..3063
                     /locus_tag="VN478_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012899501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HWQ22404.1"
                     /translation="MPELPEIANLARQMDHEFRGRRVADVDVVQPKCLNVPPEEFKRM
                     LVSTEVNRVTSRGKWIFMDLAPGTTLLLCLGMGGEALCHSKGEPLPANRQSALLFEDG
                     SAFSQHFWWFGYVHAVKTSELASHTMTSSLGLNPLEDHEFSLDAFDRMLERKKRTAIK
                     TVLLDQKNVVGIGNVYIQDILFTARLHPLRKTADVSRTERTALYGAIRSQLRNAFELG
                     GLAYEKDLYNQPGRFKDFLVGYREGKPCPVCGTIVQKIRTGSTSSFICPLCQT"
     gene            3110..>4240
                     /locus_tag="VN478_06730"
     CDS             3110..>4240
                     /locus_tag="VN478_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22405.1"
                     /translation="MKGLEDAYMAAFWARQRAQWSLYSGEPEIVAADTGLSAARNILE
                     CPTLPDRRSFVLHLAARRDAVDLEPAVKALRDQLDSDEGYDAGLDLHEKAARMTPDVL
                     QLMRLREAASVDLGAESFPALALASEELDAHWLSHFLDDFLEKNLPTARGLAASLGLN
                     PVNWFRELDSLAGPPPLADPMDLWTQLVEMLRPGELPAKPSLVVEDRELAGYTGVLHV
                     PDDVRILARPAKSLHQWLTLAHEMGHALRHLSCRQYGVLATWTTDDDETSAIIVEHLV
                     SAALLPDALARPAGDIQLLEAVRCAISARFELDLWENQDSAQQLYSMWYSRLMERPLD
                     ATLWALDSFRSIDPMCVFSYVVGYHAATQALHMNIRGPELVQF"
BASE COUNT          772 a         1363 c         1262 g          843 t
ORIGIN      
        1 atcatgaggt gcgtgcagac cacgcacgga tcgcagaggg gacgcttcag gagtcgtact
       61 gacaacgatt ggactccggc agacctgccg gacgcgggac agactcggtt gacgctgatc
      121 aggtcaggag ttcagtcgaa agcgatccag gaggcgcccc ctgtagcacg tacttcttca
      181 taggtagtca ttgaggttcc tcccttccgg ccatgggcca atgtctgttc catccgtgat
      241 tttacgcggc ggtcgcgact tgtcaagcga actcggtctg tcagtctcct gcagcgacgc
      301 gcggtccgcc accatatcag gcatatactg ttcgggtgcg ccccgactgg acgccgggag
      361 gaagatgtgc cctttgtcgc aactgctgtc ctgtttggtc tggtggtcct ggcgacccat
      421 ttcatccagg gtatcaccgg ctttggctgc gccgtgctgg ccatgccgtt ctgcatcctt
      481 ctgaccggca tcgacactgc tcgccctgtc cttacagtgc tggcgctcgt tctggacgtc
      541 tacctgcttg cagtctcgtg gaagcatgtc aactggcggc agtacctgcg catactggcc
      601 ttcgtgcttc cagggctccc cgtaggcatg gtcgcctaca acatcctgcc agagaccaca
      661 ctaatgcaca tcctcgccgt cttcatggtc gtcgtcggca tccagggctt ggtcacgcag
      721 ttccggccca acagaggcgc gcctcgacca tggcctgcat ggcttctcga cagtctgctc
      781 tttatcggcg gcgccatgca gggagcattc acctcgggag gtccgccggt catcatctat
      841 gcgacccaga agctgaagga caaggcgtca ttccgtgcaa cgctggtcgc catctggacg
      901 accctcaatg tgttcatcat cacagatatt gtcgtgcgcc acaagctgga cggcgttccg
      961 ggcaagctcg tcctgacgtc cctgccgttc ctggccctgg gagccctgct gggagacatc
     1021 gcacatcgcc acataagtgg tgagcgcttc accaggctca tctacgctgt actcctcatc
     1081 tcggccgtgt tcatggtgct atagggagta caatgatggc gctagataca tcccaggagg
     1141 cgagcaacat gattgcattt acgagaggcg ttcccgcgac agaagcgttt ccggtggagg
     1201 cactgggaga gtgtgcctcg tcagctctcg ccaagtacag ctcacagatc ctgcagtatg
     1261 gctccttccg cggattcggt ccactgcgcg agttcattgc ggcggggcac ggactggtcc
     1321 ccgaccaggt cctgctgagc cagggggcac ttcagatcgt ggatttcgcg gcccgctcac
     1381 ttatcacgcc gggcacgacc gtcctggtcg agcagccgtc gtatgaccgt gtcatcaagg
     1441 tcctgcgccg ggcgggcgcc acggtcgtgg gcgtccccct ggaggcagac ggcatggacc
     1501 tcgcagcttg tgaggatctc attcgcgaac accatccgct catggcctac gtcatcagcg
     1561 atttccagaa cccttccggc acaacgatgt ccgagaccag gcgcagagct ttcgctgcgc
     1621 ttgcagaccg gtacaacctg ctcattctgg aggacgtccc ttatcgacgc cttcggtacg
     1681 ccggcgaaga tgtccccacg atccggtcca tcgttccaca gcggactatc cagctcacca
     1741 gcttcagcaa gttgctgtcg cccgggctgc gcgtcggcgt ggcctttggc ccccagccgt
     1801 acatggaccg cctcggcagg atggcgcagg atacctacat cacgcccgcc ctcctgcccc
     1861 aggccacagt ctttgagttt atcgacaggg gcttgctgcc cgcccagata gagaagctcc
     1921 acggtctcta ccgaccacgc ctgtccagac tgctgggcgc cctcgacgcc ggcatgtctg
     1981 atctggcgac ctgggccagg ccggagggag gcttcttcgt gggcgtcacg ctgaagaagg
     2041 acgtcaaggc cgaggccctc atcgccaggg ggctggaaga aggaatccag ctcacgaacg
     2101 gacgcgactt cttcgccgac gggagaggcg acacgttcgt gcgtctgccg ttctgcgcgc
     2161 tgaccaccga ggaaatcgac gagggagtca ggaccctggc ctgggtcgtg cgttccctgt
     2221 agctgatccc actgcgagga ggctcacatg cccgaactgc ccgagatcgc caatcttgcc
     2281 cgccagatgg accatgagtt ccgtggcagg cgcgtagccg acgtggacgt cgtacagccc
     2341 aagtgtctga acgtgccgcc ggaggaattc aagcgaatgc tcgtcagcac tgaggtgaac
     2401 cgtgtgacat caaggggaaa gtggatcttc atggatctcg cccccgggac gacactcctg
     2461 ctctgtctcg gcatgggcgg agaggcactc tgtcacagca agggggagcc cttgcccgcg
     2521 aaccgtcagt cggctctcct gttcgaagac ggcagtgcat tcagccagca cttctggtgg
     2581 ttcgggtacg tccacgccgt gaagacctcc gagctggcct cccacacgat gacatcctcg
     2641 ctgggcctca atccgctgga agaccatgag ttcagtctgg atgcgtttga ccggatgctg
     2701 gaacggaaga agcggacagc gatcaagaca gtcctgctgg accagaagaa cgtcgtgggc
     2761 atcggcaacg tctacatcca ggatatcctg ttcaccgcca gactgcatcc acttcgcaag
     2821 accgccgacg tgtcgcggac cgagcgcact gccctgtacg gcgccatccg ttcacaactg
     2881 cggaacgctt tcgagctggg tggactggcc tatgagaagg acctgtacaa ccagccgggc
     2941 aggttcaagg atttcctggt cggctaccgc gaaggcaagc cgtgccccgt ttgcggcacc
     3001 atcgtccaga agatccggac tggaagcaca tcgtccttca tctgccccct ctgccagacg
     3061 tagcgcggcg cgtggcgaag gcccatccgt gtcgacagcc ggtaccagaa tgaagggcct
     3121 tgaagatgca tacatggcag cgttctgggc tcgtcagcgt gcacagtgga gcctgtattc
     3181 gggcgagccg gagatagtag cagctgacac tggactttcg gccgcccgga acatcctgga
     3241 atgtccgaca ctcccggacc gtcgctcgtt cgtcctgcat cttgccgccc gtcgcgacgc
     3301 tgtggacctg gaacccgctg tcaaagcttt gcgtgaccag ctcgactcgg acgagggtta
     3361 cgacgccggc ctcgatctgc acgagaaagc agcgcgcatg actccggatg tcctgcagct
     3421 catgcgtctt cgcgaggcgg cctccgtcga tctcggcgct gagtccttcc cggcattggc
     3481 tctcgcctcg gaggaacttg acgcgcactg gctgagtcac tttctggacg acttcctcga
     3541 gaagaacctt ccaaccgcac gcggccttgc ggcatcgctc ggtctgaacc ccgtcaactg
     3601 gttccgcgaa ctggacagtc tggcgggccc gccgccgctt gcagacccta tggacctgtg
     3661 gacgcagttg gtcgagatgc tccggcccgg cgaactcccg gccaagcctt cactcgttgt
     3721 agaagatcgt gagctcgccg gctacaccgg ggtcctccac gtgcccgacg acgtccgcat
     3781 ccttgctcgt ccggcgaagt ctctgcatca gtggctgacg ctggcccatg agatggggca
     3841 cgcactgcga catctttcgt gcagacaata cggggtcctg gccacctgga cgaccgacga
     3901 cgacgagacc agcgcaatca tcgtggagca cctggtctcc gctgcgctgc tgcccgacgc
     3961 gcttgccagg ccggccggtg acatccagct cctcgaggca gtgcgctgcg ccatcagcgc
     4021 caggttcgag cttgacctgt gggagaacca agacagtgct caacagctct attccatgtg
     4081 gtacagccgg ctgatggaaa ggcctctcga cgccacactg tgggcactcg actcattcag
     4141 gagtattgat ccgatgtgcg tcttctcgta tgtcgtcggc tatcacgccg caacgcaggc
     4201 tcttcacatg aacatccggg gtccagagct ggtccagttc
//
LOCUS       DATMZD010000325         3656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_10003,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000325 DATMZD010000000
VERSION     DATMZD010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10003"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1169
                     /locus_tag="VN478_06735"
     CDS             <1..1169
                     /locus_tag="VN478_06735"
                     /inference="COORDINATES: protein motif:HMM:NF018613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWQ22406.1"
                     /translation="TEVTDYKVLLAYASVGAGHEQAAHALGEVFEEKGWTTRYVDFLD
                     EVPVAMRFVMCDAYLQLIKILPEAYDLAFQHTVDQDFKEAEATSRLLSNVGYRRLREL
                     ALMEAPDAMISTNIWPTLALSKVKRHRMQGTPLINCFTDYVLQTLYMPRGVNWHVSAN
                     EQVTASFQAAHRRFHEPIYSFGIPVRPAFAVHHSRQEARSLLGIPQDAHLAVIAGGGL
                     GLGHILEACDALTSRPFGEPVHVVALCGNNTDLLNQVGKLAVARSSSHTITAVGWTDQ
                     IPTYMQATDLLVSKAGGVTLAEAAAVGVPLVIYQPLPGQETVNVHFLTQHQAAYAAED
                     PDELLRAADELMFTGKGQEMSGNLRRLGRPNASRDIVTKVCEDVRRPPSPAVSA"
     gene            1166..1666
                     /locus_tag="VN478_06740"
     CDS             1166..1666
                     /locus_tag="VN478_06740"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HWQ22407.1"
                     /translation="MTELLPSWARLLPKHFTASGFVLRGDRILLVNHRKHRVWIYPGG
                     HVEPDETPDEACLREIFEETGVQCRIVSPRDETLGEPGVVEVLHVPWMVLCERIPAGR
                     EPEHCHIDFAYVCEPLPGEGAHLAEDTRETEGVGWFTLEETASLTLFPDFRRQITKLL
                     KERKPD"
     gene            complement(1670..2359)
                     /locus_tag="VN478_06745"
     CDS             complement(1670..2359)
                     /locus_tag="VN478_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HWQ22408.1"
                     /translation="MSSSKSRIQVTRDGPYLVSAGVRLLKATIVVDENGVADAWEETE
                     KTKTPEDCALCRCGASGNKPFCDGTHTRIRFDGTETAGNVPYERSSQAIRGPTLVLRD
                     KTELCIGAGFCDKGLGTWESTKRSGTAAARELAIRTSGNCPSGRLVCYEKETGKPIEP
                     AYEQSVALVEDPREGLSGPIWVRGKIPVQSSEGKEYEPRNRVTLCRCGASGNKPFCDG
                     THIDVKFSDGK"
     gene            complement(2397..3212)
                     /locus_tag="VN478_06750"
     CDS             complement(2397..3212)
                     /locus_tag="VN478_06750"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015171985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HWQ22409.1"
                     /translation="MNQEAGSRKAQIVYFCHGGGPLPILGDPSHKAMVDFMTQLPSQL
                     ARPDAILVISAHWEEKVATLLGAQSPPMFYDYYGFPDKAYEITYPAPGSPGLADTIAG
                     LLKKDNIAVHVDPQRGFDHGLFIPLKLMYPEANIPCLQLSLIRGLDPAAHIALGKSLR
                     GLMNENILVIGSGFSFHNLGAFFGGDFGAPDPANEAFQDWLIETCTSPSLDERQREQR
                     LTLWEAAPSARYCHPREEHLLPLHVCLGMAGEPARLIFDDRVLGKRGVAFLWQ"
     gene            complement(3279..>3656)
                     /locus_tag="VN478_06755"
     CDS             complement(3279..>3656)
                     /locus_tag="VN478_06755"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HWQ22410.1"
                     /translation="ADSKARGMEPGSAIMTARVMEHAPVTIFVFNPEGTHPWTPHTVG
                     QMFLEAVDTQSVGAAIQNMLLAAQGIGLGTLWMCDVWSAYQQLSAWLGESGELIAAVA
                     LGYPDEQPAARPRRPLPEVVRWF"
BASE COUNT          669 a         1189 c         1090 g          708 t
ORIGIN      
        1 gtacagaagt gacggactac aaggtccttc ttgcgtacgc gagtgtaggc gccggacatg
       61 agcaggcggc tcatgcgctg ggagaggtct tcgaggagaa gggatggacg acgcgatatg
      121 tcgacttcct cgacgaggtc ccggtggcga tgcgcttcgt catgtgtgac gcctacctgc
      181 aactcatcaa gatcctcccg gaagcgtatg acctggcctt ccagcacacg gtcgaccagg
      241 acttcaagga ggcggaggcc acgtcacggc tgctctccaa cgtcggctac cggaggctgc
      301 gggagctggc gctcatggag gctcctgacg ccatgatctc tacgaacatc tggccgaccc
      361 tggccctctc gaaagtcaag cgccaccgca tgcagggaac gcctctcatc aactgcttca
      421 ccgactatgt cctgcagacc ttgtacatgc ccaggggcgt caactggcac gtctcggcca
      481 acgagcaagt gacggcgagc ttccaggctg ctcaccggcg tttccacgag ccgatctact
      541 cctttggcat tcccgtccgt ccggcgttcg ccgtccacca ttcgcgtcag gaagcccgca
      601 gcctgctggg tattccacaa gacgctcatc tggctgtcat cgcaggcggg ggacttggcc
      661 tcggtcacat cctggaggcg tgtgacgcct tgacgtcccg gccctttgga gagcccgttc
      721 acgtcgtcgc cctttgtggc aacaacacgg acctcctgaa ccaggtaggg aagctcgcgg
      781 tcgcccgctc atccagtcac acgatcacgg ccgtgggctg gactgaccag attcccacat
      841 acatgcaggc aactgatctg cttgtcagca aggcaggcgg cgtcactctg gccgaggcag
      901 ccgctgtcgg ggtcccgctc gtcatctatc aacccctccc gggtcaggag acggtaaacg
      961 tccacttcct gactcagcac caggcggcgt atgcggcaga ggaccctgac gagctcctgc
     1021 gtgccgccga cgaactgatg ttcacgggga agggtcagga aatgtccggc aacctcaggc
     1081 gcctgggaag acccaacgcc agccgggaca tcgtcacgaa ggtctgtgaa gatgtgcgtc
     1141 gcccgccttc gcccgcggtg tctgcatgac cgaactgctg ccgtcctggg cacgtctgct
     1201 gcccaagcac ttcaccgcgt cgggctttgt tctgcgaggt gatcgcatcc tgctggtcaa
     1261 tcatcgcaaa catcgtgtct ggatctatcc tggcgggcac gtggagccgg acgagacgcc
     1321 ggacgaggcg tgccttcgcg agatcttcga ggagaccggc gtgcaatgcc gtatcgtgag
     1381 cccccgggac gagacgctgg gcgagccggg cgtcgtcgaa gtcctgcatg tgccatggat
     1441 ggtcctgtgt gagcgcatcc ctgccggcag ggaacccgaa cactgccata tcgacttcgc
     1501 ctacgtctgt gaacccctgc ccggcgaagg ggcgcatctt gcggaggaca cccgcgagac
     1561 ggaaggcgtc ggctggttca cgctcgagga aacggcctcg ctcacgctgt tccccgactt
     1621 tcgccgccag atcaccaaac tgctgaagga acgaaagcca gactagaacc tacttcccgt
     1681 cagagaactt cacgtcgata tgtgtgccgt cacagaatgg cttgttgcct gatgctccac
     1741 acctgcacag tgtcaccctg tttctcggct cgtactcctt gccttccgat gactgcaccg
     1801 gaatcttccc tcgtacccag attggaccgc tcaaaccctc ccgagggtcc tcgacaagag
     1861 cgacggactg ctcatatgca ggctcgatcg gttttccggt ctccttttca taacacacca
     1921 gcctgccgga cgggcaattg ccggacgtcc gtatggccag ctccctggca gctgcagtgc
     1981 ccgagcgctt cgtcgactcc cacgtgccaa gccccttgtc acagaaccca gcaccaatgc
     2041 agagttctgt cttgtccctg agaaccaggg tggggccgcg tatcgcctgc gagctccgct
     2101 catacggcac attcccggca gtctctgtac cgtcgaatcg gatccttgtg tgcgtgccgt
     2161 cacagaatgg cttgttgcct gatgctccac acctgcacag tgcgcagtct tcaggggtct
     2221 tggtcttctc cgtctcttcc catgcgtccg caacaccatt ctcgtcgaca acgatggtcg
     2281 ccttcagcag acgaacccct gcagacacga gataggggcc gtccctggtg acttgtattc
     2341 gagacttgct gctgctcatg ttgtttcccc ttccagtctc tccaccagcc tgtgcctcat
     2401 tgccacagga acgccacacc acgcttgccc aggacccggt cgtcgaatat caacctggcc
     2461 ggctctccgg ccatgccaag gcagacgtgc agtggcagca gatgctcctc gcgggggtgg
     2521 cagtagcgcg cggagggagc tgcctcccac agggtcaagc gctgctcgcg ctggcgctcg
     2581 tcaagcgaag gactggtgca ggtttcgatc agccagtcct gaaatgcttc gttggcaggg
     2641 tccggcgcgc cgaaatcccc tccgaagaag gctcccaggt tgtgaaatga gaatccggat
     2701 ccgatgacca ggatgttctc gttcatcaga ccgcgcaggc tcttgccaag ggcaatatgc
     2761 gcagccggat cgaggccgcg tatcagcgac agctggagac acggtatgtt ggcctcggga
     2821 tacatcagct tgagcgggat gaacaggccg tggtcgaatc cgcgctgagg gtcgacgtgg
     2881 accgcaatgt tgtccttctt cagcagcccg gcaatggtat cggccagccc ggggctgccc
     2941 ggggcggggt aggtgatctc atacgccttg tcggggaaac cgtagtagtc atagaacatc
     3001 ggcgggctct gtgctcccag cagcgtcgcc accttctcct cccagtgggc gctgatcacg
     3061 aggatggcat ccggtcttgc aagctgcgac ggcagctgcg tcatgaagtc caccatcgcc
     3121 ttgtggcttg ggtccccgag gatcggcagg gggcctccgc cgtggcagaa gtagacgatc
     3181 tgggctttcc tgctgcccgc ttcctggttc atcgagcatc ccctttcgcg cagaagtgct
     3241 catcagcctc gcggctgacg agagaaaaag acaacctgct agaaccagcg cacgacctcg
     3301 gggagggggc gccttggacg cgcggcgggc tgctcgtccg gatacccgag cgccacggcg
     3361 gcgatcagct caccggattc tcccagccac gccgacagct gctggtatgc ggaccatacg
     3421 tcgcacatcc agagtgtgcc cagaccaatc ccctgcgccg ccagcagcat gttctggatc
     3481 gcggccccga cggattgcgt gtctaccgcc tcaaggaaca tctgtccaac cgtgtgcggc
     3541 gtccagggat gggtgccctc ggggttgaag acaaagatcg tcaccggggc atgctccatg
     3601 acgcgcgccg tcatgatagc cgagccgggt tccatccctc gcgccttgct gtcggc
//
LOCUS       DATMZD010000326         3047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_130390,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000326 DATMZD010000000
VERSION     DATMZD010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130390"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1739)
                     /locus_tag="VN478_06760"
     CDS             complement(<1..1739)
                     /locus_tag="VN478_06760"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HWQ22411.1"
                     /translation="MKTSERNTVRTFILLRYAVIAAGSVWWLVAMLGRTPQGVQLVLL
                     PAILVTGIAFNVSLDLMDRFAPHRGVASAILRHQVLFDGVAVNLFLGAMGDRLLMGKP
                     VFYDGTRILPFDASFVVSSALVVVASIVLHNGHFPVYLAAALAGLVASQGLSIAQQQG
                     IGFVQALQSANWGYVATGMLATASIGALAYGADEYVRRSLHTMDARVADVSRDKERMQ
                     ASFHKLSFLTAIIRDMAASQAYQQVLEAITDRAALFFSADDAIIATVDADAEHLSVVA
                     ARSEFREALSHLRIRRGEGILGKIFDGDTPELVKNALLDPRAMQIYGTPEEPESMMVA
                     PLRKGSQKYGLVSVSRIGVENPFSEEDLSLFTSFANIAASVLDNATIMEALKRKNFTL
                     TVTNQLSSAIVSPLPFDEEINSFLSIMNNTFQLSRINLLMVENDRFTRFFAVPPPKSA
                     VTAQETIARMNTGAGVLGQALRERTIVNVPNVADYANYIAADQATRSELAIPMKNAEG
                     RVIAVLNLESGQTDYFKNDVSADVLTVAAEIQGFLQGRLIWEEVKEQRNMREAINRAE
                     LENIDVSTPEQATA"
     gene            complement(1859..2095)
                     /locus_tag="VN478_06765"
     CDS             complement(1859..2095)
                     /locus_tag="VN478_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22412.1"
                     /translation="MTIGHSDDELDQLLREHYHAVIDGVTAPEHLLRQALTETRPGGG
                     RETRWTPVLAFALAGASFLIILASSRAIEVLLSL"
     gene            complement(2076..2564)
                     /locus_tag="VN478_06770"
     CDS             complement(2076..2564)
                     /locus_tag="VN478_06770"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HWQ22413.1"
                     /translation="MNSCADEIYAYLVARMGNRFDADDVFQSTFFKVRRFLPTFRGDS
                     SPTTWVMRIAMNEASSFRRHTGRELPLLTPDDALPPSDSDEAAYDPVRDETERDVAAG
                     MKHLQGDLRDVLYFYYYKEMPYERIAQLIGKPVGTVKSRLFRAKEELRTFMEAHRDHR
                     TQ"
     gene            complement(2594..>3047)
                     /locus_tag="VN478_06775"
     CDS             complement(2594..>3047)
                     /locus_tag="VN478_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22414.1"
                     /translation="FGIIPGLITARLTGLLGGVVVAPLVMLFMVRYEAYRLPTKLSWF
                     KAFLVSAISFLLLFGPLILPRTTALLRFSLARGTLVQLLPWAALWFVTAFVLACLIVA
                     AVLELSFREAMSWWGVAFAAALAISWIGALACVLLYRYLILPSLTDFF"
BASE COUNT          631 a          862 c          967 g          587 t
ORIGIN      
        1 gccgttgcct gctccggcgt cgatacgtcg atgttctcca gttctgcccg gttgatggct
       61 tcgcgcatgt tccgctgttc tttcacctct tcccagatca gccttccctg caggaatccc
      121 tggatttctg cagccacagt gagaacgtcg gccgagacgt cgttcttgaa gtagtccgtc
      181 tggccgcttt ccaggttgag cacggcgatg acccggcctt cggcattctt catgggaatg
      241 gcgagctcgc tgcgtgtggc ctgatcggcc gcaatgtagt tggcatagtc ggctacgttc
      301 gggacattga cgatcgtccg ctcgcgcagt gcctgtccaa ggacacctgc tcctgtgttc
      361 atgcgggcta tggtttcctg agccgtgacg gcggacttcg ggggaggcac cgcgaagaac
      421 cttgtgaagc gatcgttctc caccatgagc aggttgattc gggagagctg gaacgtgtta
      481 ttcatgattg acaggaagct gttgatctcc tcgtcgaacg gcaaaggcga gacaatggcg
      541 gaggatagct ggttggtgac cgtcagcgtg aaattcttgc gtttcagagc ctccatgatc
      601 gtggcgttgt ccaggacgga ggccgcgatg ttcgcaaatg acgtgaacag cgagagatcc
      661 tcctcgctga acgggttctc gacgccgata cggctgacag aaaccaggcc gtacttctga
      721 ctgcctttgc gcaagggagc gaccatcatc gattctggct cttccggggt cccgtagatt
      781 tgcatggcac gcgggtccag cagggcgttc ttgacgagtt caggggtgtc gccgtcgaag
      841 atcttgccaa ggatcccctc tccacgccga atgcgcaggt gggaaagggc ctcgcgaaat
      901 tcgcttctgg cagcgacgac cgagaggtgc tccgcatcag cgtcgacggt agcgatgata
      961 gcgtcgtccg ccgagaagaa cagggcggct cggtcagtga tggcctcgag gacctgctgg
     1021 taggcctgag atgcagccat gtcgcggatg atggcggtga ggaaggacag tttgtgaaag
     1081 gaggcctgca tgcgctcctt gtcacgactt acgtcggcca cgcgggcgtc catggtatga
     1141 agactgcgcc tgacgtactc gtccgcaccg tatgccaggg cgccgatgct cgccgtggcg
     1201 agcatccccg tggcgacgta tccccagttg gccgactgga gcgcctgcac gaagccgatg
     1261 ccctgctgtt gggcgatcga caatccctga ctggcgacaa gacctgcgag ggccgctgcg
     1321 aggtacacgg gaaagtgacc gttgtgcagg acgatcgacg cgacgacaac cagggcactg
     1381 ctgacgacga agctggcgtc gaacggcagg atacgcgttc catcatagaa gacaggcttg
     1441 cccattagaa ggcggtcacc catcgcaccc aggaacaggt tcactgcaac gccgtcgaac
     1501 aggacctggt ggcgaaggat cgcacttgcg acaccacggt gcggcgcgaa acgatccatg
     1561 agatccagcg agacgttgaa ggcgattcca gtgacaagta tggccggcag caggacaagc
     1621 tgaacgccct gaggggtacg gccgagcata gcgacaagcc accagacgct tcccgcagca
     1681 atgactgcgt accggagcag tatgaacgtc ctgacggtgt tgcgctcgct agtcttcatg
     1741 gtctttctac cctctccata gaaaaactat aacgatggtg cggaaaaagc aagccgcagt
     1801 gcaagtgggg caccgccccc tccgggtatt cggaacagac tgcgaagcag gcggtggtct
     1861 agaggctgag cagaacctcg atggcgcggg aggatgccag gatgatgagg aaggatgcac
     1921 cggccagagc gaaggccagg acgggcgtcc agcgggtctc acgcccaccc cccggtcgtg
     1981 tctctgtgag tgcctggcgc agcagatgtt ccggcgcagt caccccgtcg atcacggcgt
     2041 gatagtgctc ccgaagcaac tggtcgagct cgtcgtcact gtgtccgatg gtcacggtgt
     2101 gcctccatga aggttctcaa ctcttccttc gcgcggaaca ggcgcgattt gacagtcccc
     2161 accggcttcc caatgagttg ggcgatcctc tcgtagggca tttccttgta gtagtagaag
     2221 tacaggacgt ctctgaggtc gccctgaagg tgcttcatcc cggccgcgac atctctctca
     2281 gtctcatctc gtaccgggtc gtacgcggcc tcgtccgagt ctgacggagg cagcgcgtca
     2341 tccggggtca gcaggggaag ctcccgtcca gtatgccggc gaaagctgct ggcctcgttc
     2401 atggcaatcc tcatgaccca cgtcgtcgga ctggagtcgc cgcgaaaggt ggggagaaag
     2461 cgacggacct tgaagaacgt ggactggaag acgtcgtcgg catcgaaacg gtttcccatt
     2521 cgtgcgacca ggtatgcgta gatttcgtct gcacaactgt tcatgagtgt ctcgaaatcc
     2581 agcagcatcg gtgctagaag aaatcggtca gacttggcag gatgaggtat cggtacaaca
     2641 ggacgcaggc tagcgccccg atccagctga tcgcaagtgc tgcggcgaag gccacgcccc
     2701 accatgacat ggcttccctg aacgacagct ccaacactgc cgctacgatg agacatgcga
     2761 gtacaaatgc ggtgacaaac cagagggctg cccatggtag tagctggacg agcgtgccgc
     2821 gggcgagtga gaatcggagt agtgccgtcg tgcgaggcag gatgagcggt ccaaacagca
     2881 ggagaaatga gatggccgac acgaggaatg ccttgaacca ggacagcttt gtgggcagac
     2941 ggtatgcctc gtagcgcacc ataaacagca tgacgagtgg tgcgacgacc acgccgccaa
     3001 gcagtccggt cagtcttgcc gtgatgagtc ccgggatgat gccgaac
//
LOCUS       DATMZD010000327         2158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_130405,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000327 DATMZD010000000
VERSION     DATMZD010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130405"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(232..1485)
                     /gene="tyrS"
                     /locus_tag="VN478_06780"
     CDS             complement(232..1485)
                     /gene="tyrS"
                     /locus_tag="VN478_06780"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015043996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HWQ22415.1"
                     /translation="MEKTQSEQEVQRQLAVLRRGTAEIVNEEGLAAKIRSSLASGKPM
                     RVKLGADPSAPDLHLGHAVVLDKLRQFQDLGHQVVFIIGDYTARIGDPSGRSKTRPPL
                     TGEQIDANARTYFEQVYRILDRQKTEVRYNGEWLSTLSFEEIIRLASRFTIQQILERD
                     DFSKRYHSQVPIFFHEFFYPLMQAYDSVAVEADVELGGTDQKFNFLLARELQEQVGQV
                     AQVAFMMPILPGLDGVHKMSKSLGNYIGIAEGAVDMYGKCMSIPDGLMESYFTLLTMM
                     PEQEIHATVAGIADGSVHPMTAKKRLAFLVTERFHGTEGASAAQANFENVFSKRASSA
                     SSEIAYEDVSLPAEFAGLSDVAVIDLLFGLSAVPSKSEARRLIQQGGIEVEGLRVADI
                     GFRVSLERSPVHMRVGKKRFLAITF"
     gene            complement(1497..>2158)
                     /locus_tag="VN478_06785"
     CDS             complement(1497..>2158)
                     /locus_tag="VN478_06785"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HWQ22416.1"
                     /translation="PAAATIVFAGGDPGPMSYLRALDYSGALLVAVDRGLSVMVELGL
                     TADLFVGDGDSVSPELLSGLDKGRTRIVMLPAHKDISDLEAAFDLLAAMERHDSVLVL
                     AGLGGRLDHCLFNLQLAARHRGDFEDITFEDEASLVRPLTGSTTLDLPCGTVVSFVPV
                     TGEVRLSLVGFEYPLAHALVQSGSTRTLSNVSRGPVQQVVVEEGTALMIVSKVRSPIT
                     A"
BASE COUNT          429 a          716 c          596 g          417 t
ORIGIN      
        1 ctccaattcc tcccccgata gggatgttca gcgacatcag gcgcgtcagg acgatgctca
       61 gcgcaatcat gatgccgtcc agggtcaagg ccctcagaac cacgccggta cgatccagct
      121 tgctgtcgac gggtacgccc acgctgccac ctcccgcatg cccacaatct catcgccgcc
      181 aagtagactg aagtgcgcat ctactcggcc agcgcatgcc accgcgcctg tctagaacgt
      241 gatcgccagg aatcgcttct tgccaacccg catgtggact ggcgagcgct ccagactgac
      301 ccggaaccct atgtcagcga cacgcagtcc ttccacctcg atgcctccct gctggatcag
      361 gcggcgcgcc tccgacttcg acggtaccgc actcaggccg aacagcaggt caatgaccgc
      421 aacatcggac aatccggcaa actctgcggg tagcgagacg tcctcatagg cgatctcgga
      481 cgatgcactc gacgctctct tgctgaacac gttctcgaag ttggcctgtg ctgccgacgc
      541 gccctctgta ccgtggaagc gctcagtgac gaggaatgcc agacgcttct tcgcggtcat
      601 ggggtgcaca gagccgtctg ctatcccggc caccgtagcg tgtatctcct gctccggcat
      661 catcgtgagg agggtgaagt atgactccat cagtccatcc gggatcgaca tgcacttgcc
      721 atacatatcc acggcaccct ctgctatgcc tatgtagttg cccagactct tgctcatctt
      781 gtgtacgccg tcgagccccg gcaagatggg catcatgaac gccacctgcg cgacctggcc
      841 gacctgctcc tgcagctcgc gagcgagaag gaagttgaac ttctgatcag tgccccccag
      901 ctcgacatcg gcctcgaccg ccacggagtc atatgcctgc atcaatggat agaagaactc
      961 gtggaagaag atcgggactt gactgtgata ccgcttcgag aagtcgtcgc gttcgaggat
     1021 ctgctggatg gtgaaccgcg acgcgaggcg gatgatctcc tcgaacgaca acgtggacaa
     1081 ccactcgccg ttgtagcgga cttcggtctt ctgccgatcg agaatgcggt acacctgctc
     1141 gaagtacgtc ctcgcattgg cgtcgatctg ttcaccggtg agcggaggac gtgtcttcga
     1201 acgacctgac gggtccccga tcctggcggt gtagtcgccg atgatgaaca cgacctggtg
     1261 gcccagatcc tggaactgcc gcaacttgtc gagtacgacg gcatgtccca gatgcaggtc
     1321 aggtgcgctt ggatccgctc cgagcttcac ccgcatcggc ttccctgagg ccagggaact
     1381 cctgatcttc gccgccaggc cctcctcatt gacgatctct gcagttccgc gccggaggac
     1441 agccagctga cgctgcactt cctgctccga ttgtgtcttt tccatatcca cctctctcat
     1501 gcagtgatag ggctgcgtac cttcgagacg atcatgagcg ccgtcccctc ttccacgacg
     1561 acctgctgaa caggcccacg cgagacattg ctgagcgttc ttgtcgaccc actctgaaca
     1621 agtgcatgag caagagggta ctcgaatccg acaaggctca gccggacttc cccggtcacg
     1681 ggcacaaacg agacgaccgt gccgcagggc agatccaggg tcgtcgaacc ggtcagcggc
     1741 ctgacgaggc ttgcctcatc ttcgaacgtg atgtcttcga agtcgcctcg atgccttgct
     1801 gcgagctgga ggttgaacag gcagtgatca agtctccctc cgaggcccgc cagcaccagc
     1861 acgctgtcgt gtctctccat cgccgccagc agatcgaatg cggcctcgag gtcggagatg
     1921 tccttgtgtg caggcagcat gactatccgt gtccgaccct tgtccaaacc cgacagcagt
     1981 tccggagaca ccgaatcgcc atcccccacg aacaggtccg cggtcagtcc cagctccacc
     2041 atgacgctca gaccacgatc tacggccacc agcagcgcgc cggagtagtc cagcgccctg
     2101 aggtacgaca ttggtccagg gtctcccccg gcaaagacga tcgtcgccgc ggcaggca
//
LOCUS       DATMZD010000328         3089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_10025,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000328 DATMZD010000000
VERSION     DATMZD010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10025"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VN478_06790"
     CDS             complement(<1..375)
                     /locus_tag="VN478_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22417.1"
                     /translation="MKRLIVVLICMSLVLGLVPIPLRMPVARAAAVPSVALSIPSETM
                     IGESVTFTATFDNTSGDTGYGPFIDLVFPYNGMDGAHGTATPDGMSFVSATYLGASVT
                     ATTLTFPDDDGAGAGTTGHVLHP"
     gene            809..1159
                     /locus_tag="VN478_06795"
     CDS             809..1159
                     /locus_tag="VN478_06795"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HWQ22418.1"
                     /translation="METPRKGDVWLVNFDPTCGKEIQKKRPAVVVSRDEYNAFAWPVL
                     VVPLTSQGLGEVTPVFEVPVPRGEGGTEHDSKTKATHMRTVDRSRLLELLGHVDDATM
                     RGIEQTIRNLIDMA"
     gene            complement(1258..1713)
                     /locus_tag="VN478_06800"
     CDS             complement(1258..1713)
                     /locus_tag="VN478_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22419.1"
                     /translation="MATLASTTTTPATPTSTGPSFFVLLLSGLAIMFLGNLLGRFLST
                     RTGKTVSRKLYFMITIPIMVIFVVIVFAMGRSLTVIGQYALFAAYILGFSILGGFVEA
                     PPMPTRTAGPSREPKDGEELPHMATGSDTVEGEGKDITQDSPVDDKTEH"
     gene            1821..2300
                     /locus_tag="VN478_06805"
     CDS             1821..2300
                     /locus_tag="VN478_06805"
                     /inference="COORDINATES: protein motif:HMM:NF014711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HWQ22420.1"
                     /translation="MAVEGVSVNNLWDNKILIVAVIADLVAQGLKPLIHYGEYHEWKW
                     QLLFSNGGFPSSHSATVTALTAMVAFTQGFASPLFAVCMVFSGVVVFDASGVRQEVGR
                     HARTLNAIFQEFKLWDRFDYKQFTELVGHTLFEVVGGMALGLLIALLSLRIPFLRPR"
     gene            2305..2766
                     /gene="rpiB"
                     /locus_tag="VN478_06810"
     CDS             2305..2766
                     /gene="rpiB"
                     /locus_tag="VN478_06810"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007506157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HWQ22421.1"
                     /translation="MKIIVGSDHGGFRLKSFLIHALTLKGNEVEDLGTDSGDHSVDYP
                     DFAAAVARGVVADSTAVGIVICGTGIGASIAANKVAGIRCALVHDGYTARMAKEHNDA
                     NVLALGGRTTAEEIALDCVMTWLNTTYQGGRHQARLDKIAALECSTGSAGK"
     gene            2796..>3089
                     /locus_tag="VN478_06815"
     CDS             2796..>3089
                     /locus_tag="VN478_06815"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HWQ22422.1"
                     /translation="MKTALLLVDIQKEYFQGGAMPLEGAAEAAIQARKLLAHCRYNHV
                     PAVFIQHIALDPQAGSFRRASPGVSLYTSIRPLPGEAVVRKHYPNAFRDTNLLE"
BASE COUNT          653 a          918 c          920 g          598 t
ORIGIN      
        1 gggatggaga acatgtcccg tcgtgcctgc gccggcacca tcgtcgtcgg gaaaagtcag
       61 agtggtcgcc gtgacagacg ctccgaggta tgtcgcgctg acgaaggaca tgccgtcagg
      121 agtcgccgtg ccatgtgcgc cgtccatccc gttgtagggg aacacgaggt cgatgaaggg
      181 gccgtagccg gtatcccctg aagtattgtc gaatgtcgcc gtgaacgtca ccgactcccc
      241 gatcatcgtc tccgatggta tcgagagagc gacgctgggc actgcagcag cccgtgcaac
      301 cggcatccgc aaaggaatgg gaacaagtcc cagcacaagc gacatgcaga tcaatacgac
      361 aatcagtctc ttcatgaagt acccccgtca cggtgagatt tgtgctttgt gcccgatact
      421 cccgtagagc accgggcttt ctgtgtaatc atacggattt gcccccatga cgtcaagcga
      481 cgtgcattgc gttcttttcg gcccactcgc tcctgtcgcc gagggccaaa acattgacga
      541 gggctgcaat tgcgccatga tgagtcagac aatccaggaa acgcaggagg caccatgcca
      601 caacgaagga tcacagtaag cgtgtccgat gacatgtatc aggagctcct caggcacggc
      661 aacaagtcgg cgttcgtcag agaggcgctg caggcttgcg tcacccgcct ccggcaatgc
      721 gacgtcgctt cacagatacg agactactgc acgaaggaat cagaatccga ccgaatgttg
      781 tgttcggagc tggacggggg tacgaacgat ggaaacacct cgcaagggtg acgtctggct
      841 ggtcaacttc gacccaacgt gcgggaagga gatccagaag aaacgtccgg cagtcgtcgt
      901 ctctcgtgat gagtacaatg cgttcgcctg gcccgtattg gtcgttccac tgacgtcgca
      961 gggactggga gaggtcacgc cggtgttcga ggtgcctgta cccaggggcg aaggcggaac
     1021 ggagcatgat tcgaagacga aggccaccca catgcgcact gtcgacagga gccgccttct
     1081 ggaactcctc ggccatgtcg acgacgcgac catgcgcggc atcgagcaga ctatccgcaa
     1141 cctcatcgat atggcgtgac ctggacgaac gacaagctca tgaacgcgat caccccgctc
     1201 caaggagcgg ggtgatctac aatgttccgc ttgtatctat ctaccagctg accccgatca
     1261 gtgctcggtc ttgtcgtcga cgggagagtc ctgggtgatg tccttgccct cgccttcgac
     1321 ggtgtcgctg cccgtggcca tgtgcggcag ctcctcgcca tccttcggtt ccctggaggg
     1381 tcctgccgta cgcgtcggca tcggcggagc ttcaacgaag ccgccaagga tcgaaaaccc
     1441 aagaatgtag gccgcgaaca gggcgtactg cccgatcacg gtgaggctgc gccccatcgc
     1501 gaagacaatg acgacgaaga tgaccatgat ggggatggta atcatgaaat agagcttgcg
     1561 actgaccgtc ttgccggtac gcgtggacag aaagcgccca agcaggttgc caagaaacat
     1621 gatagcaagg ccggagagca gcaggacgaa aaagcttggc ccggtacttg tcggggtagc
     1681 cggagtcgtc gttgtcgatg caagtgttgc cacgggtatc ctcctatgaa cgcgcgcggc
     1741 gcaggagctg atgcgatccg ctgtcttggt gtgcgtttac aaactactcc atcactgtat
     1801 actcatcgtg tggtttatca atggcagtcg aaggggtgag cgtgaacaac ctctgggaca
     1861 acaagatcct gattgtcgcc gtcatcgcgg accttgttgc acaagggctc aagccactca
     1921 tccactacgg tgagtatcat gagtggaagt ggcagcttct cttctccaac ggcggcttcc
     1981 caagttctca cagtgccacc gtcacagccc tgacagctat ggtggcattc acacagggct
     2041 tcgcatctcc cctctttgcc gtctgcatgg tcttcagcgg cgtcgtcgtg tttgacgcgt
     2101 cgggcgtgcg ccaggaagtc ggcaggcatg cgcggaccct caacgccatt ttccaggagt
     2161 tcaagctgtg ggaccgcttc gactacaagc agttcacgga gctggtcggc cacaccctgt
     2221 tcgaagtagt cggaggcatg gcgcttggat tgctcatcgc gctgctgtct cttcgcatcc
     2281 cattcctcag accgaggtga atagatgaag atcatcgttg gctccgatca cggtggattc
     2341 cgcctcaagt cgtttctcat tcatgcattg acgctcaagg gcaacgaagt cgaagacctg
     2401 ggcactgaca gcggcgacca ctcggtcgac tatcccgact tcgcagccgc cgtggccaga
     2461 ggagtcgttg cagactcgac ggcagtcggc atcgtgatct gtggcactgg tatcggcgcc
     2521 agcatcgcgg ccaacaaggt cgctggtatc cggtgcgcgc tggtccacga cggctacacg
     2581 gcacgcatgg ccaaggagca caacgacgcc aacgtgcttg cgctcggcgg gcggacaacg
     2641 gctgaggaga tcgctctcga ctgcgtcatg acctggctga acacgaccta ccagggcggg
     2701 cgtcaccagg cccgcctgga caagatagca gcgcttgagt gctctacggg gtccgctggt
     2761 aagtagccag aagtggtaca cccgcgatct catccgtgaa gactgctctc ctgctcgtcg
     2821 acatccagaa ggagtatttt cagggcggcg ccatgcctct tgaaggcgcg gctgaagcag
     2881 ccatccaggc gcgaaagctg ctcgcccact gccggtacaa ccatgtaccc gctgtcttca
     2941 tccagcacat cgccctcgac ccacaggcag gctcgttcag gcgcgcctca ccgggcgtga
     3001 gtctctatac cagtatcagg ccgcttcccg gcgaggcagt cgtccgcaag cactatccca
     3061 acgcgttccg ggacacgaac ctgctggag
//
LOCUS       DATMZD010000329        12533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_119928,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000329 DATMZD010000000
VERSION     DATMZD010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 12533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12533
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119928"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1498
                     /locus_tag="VN478_06820"
     CDS             <1..1498
                     /locus_tag="VN478_06820"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HWQ22423.1"
                     /translation="SWSPERSSLCSGAVVSFITPDSRECRPGAMFVAIAGTRLDGASY
                     VAGALEAGAVCIVAETEHPEIRGVPVVVVSNCREFLSLASQAFFNFPARRMKLIGITG
                     TSGKTSTAYMAHRLLQGLGIPCVMIGTTGYVMPSGEFFAPDTLPATTPEAFSLHWYLN
                     HAVQQGAQVAVLEVSSFALAFDRVYGVRFDCGVFTNFSQDHMGYHGTMDAYLAAKLRL
                     FTSLDRDSVAVLNADDPSFAAFRDAAGGSHVRTFSLEHEADLRGQNVVSSRGRTTLQV
                     LTPSGAAFDVDLSIGPAFQASNCLAAISSVCAIEPTCDPGRAALSLAAPLYVPGRYER
                     IDCGQPFEVVVDYAHTPEEFSALFGAVRPTVRGDLLAVFGSVGADDREKRPIMARLAE
                     RVCRWSFVTVEDPRHEDASIAVRDIQTGFTTDHYTVIEDRRLAIRSAIAAAKPGDMVL
                     VLGRGHETVMYYEHENVAFDDRDEARLALSALGYRCSACGCDSDEAQQ"
     gene            1495..2475
                     /gene="mraY"
                     /locus_tag="VN478_06825"
     CDS             1495..2475
                     /gene="mraY"
                     /locus_tag="VN478_06825"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HWQ22424.1"
                     /translation="MTVMSGMSLVLVLALVDLTLFPLLIRWLQKARFTQLIRDEGPAS
                     HKSKAGTPTGGGLMLVLNIAVAVLVYAILIARAGLTKADVATLILVGVNLLGGFLDDW
                     TKELKHRNDGLLGYQKLLIQTAAAMLFFYVGFPSIRPILQTPGGRVLLAGWFFYVVAI
                     LYSVGMVNAFNLTDGLDGLLAKTSLPAFIVAAAASMLHPSGVAGVLPLAAVAFGISFL
                     WFNGTRASVFMGDTGSLALGSVFVAWAFVSGNQIPSILVGGLFLVEALSVIIQVVVFK
                     ASGRTRRVFLMAPIHHHFEKLGWAEPKIVDRFFVVSILFVIAGALTMVWR"
     gene            2479..3858
                     /gene="murD"
                     /locus_tag="VN478_06830"
     CDS             2479..3858
                     /gene="murD"
                     /locus_tag="VN478_06830"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HWQ22425.1"
                     /translation="MIPFESAFVVGALPSGLYAALYLHDHGVEVFVSESKQRSADEKF
                     ELAVLLLERAGIPFEFGQNTPDIMTRFDAVVVSPGVPPEAPIMVAAARAGKFIAGETE
                     IAAYAGLSVLAVTGSNGKSTTTALLGAIVAQEHPRVVTGGNLGTPVSQLLVEHPSAGP
                     AVLEVSCFQLETIHEFHPKVALFSNLVPNHLDRYGAMERYFATKKRLFENMTADDAIV
                     LNWDDPALRELGPTLCSRVFYFSLGDGAFPGVHLADGVIVFKDGATCVELFRETDLRV
                     PGRHNVANAMSAALAAFLYGVSPGSIQRAVAGFNGLPHRLENAGTSHGVTFVNDSKAT
                     TPESVMTAVDAMSGAYVVILGGSSKDVSFDKMARHLAGDKNLEMAIVMGATGSEIEDS
                     LRRAGMKRIVRVGSLEEAVGTGVQLLPAGGTLLLSPACASFDMFRDFEQRGDRFKEIV
                     QSAFGATMR"
     gene            3855..4943
                     /locus_tag="VN478_06835"
     CDS             3855..4943
                     /locus_tag="VN478_06835"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HWQ22426.1"
                     /translation="MKRILVLVGLLLCLGVLQNYSIAVSISAARGQLVAVTPSVVKQI
                     VIVGIGAFAYWFCSRFKYMRWADISEFLTYLLGALLVAVLIPGIGRSVNGSSRWIGTN
                     VIGLQPSQAAVLVVPLLAAKLFSGKYLNQYDRRYFRTFLLFVLAFTGLVALEPDFGTA
                     FVIAGSSIAILLGVGAPFGYWASRTVVLGALALVAVNVKASWKIRMTSFINPLAADKA
                     KGVAYQLVNSMFAVARGGVLGRGFMRSVQKFVHFPAQENDFVFAIFSEEFGLIGVTVV
                     LGVFLWIVFELFRIASRTSEPFARVYVMALGVFLAVQAFVNIGVVVGLLPTTGVPLPF
                     FSQGGNHIVFEMAALGIAGAIARSPEAV"
     gene            4943..6010
                     /locus_tag="VN478_06840"
     CDS             4943..6010
                     /locus_tag="VN478_06840"
                     /inference="COORDINATES: protein motif:HMM:NF015022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HWQ22427.1"
                     /translation="MHYVIAGGGTGGHIYPAAAIAERLSDSGRVTMLARSGSMEERIF
                     LSQGFDVRTVASAPLLYDPGSLWRLVRATGRGVRAARRVMTDVHADAFIGTGGYVSVP
                     GIIAAHLSRVPVYLLEQNTVMGRANRLFSHCARRVFLGFPVVGARGPRFALTGNPLRR
                     DVYVALTECRSSRPLRRGLLFIGGSGGADFINGLFLHTIRELDSKERDVEVTVVTGTD
                     GYTGIRDAVDKLALRHVQATVIAYEDHMERLYAHTRVAVTRGGALVLTELAVGGIYAV
                     AIPYPFSVGGHQSKNAAHLEKLGLGVRIEQSLLKFEGYLSIVEKALDDERPPEVPASS
                     VFGGDAAAAIARSIREECSGD"
     gene            6003..7421
                     /locus_tag="VN478_06845"
     CDS             6003..7421
                     /locus_tag="VN478_06845"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HWQ22428.1"
                     /translation="MTDLLRPGTAFLLVGICGAGMSSLAMLLHSIGMDVRGSDVMSET
                     AEAGRLTSLGIPVTSEGEAASSIRAPDVIVRSSAIQPGNPVLVAAQTTGCVVLHRTDV
                     LRAIASDYYLIAVSGTHGKTTTTGMIGYALAERGFAPTICVGGHIAGFDQMFPLKGTQ
                     QRRIGGRPIMVLETDESDASFLKFHPDVAVMTNIDRDHLGTYEGKFSNLVDAFARFSR
                     QCAEKGGLTVGCGDDKEVAGIVGSLPRYVLYGQGAENQVHMEYDLLTNTATVSRDGIE
                     QPFVMERGDEKSYLNAVAACLACEAVGLALPDALHVLERFPGMERRMQVLADDGQIAV
                     LTDHADHPTEIRATLRAVAARYPGRPLTLVLQPHRFSRVAMCLDGYGPALAGTSKIVL
                     LDIFPAGETVERPAELNQRLRESVESSAGASLAPRTPVDQILEDLQMTTTPGDVVLFM
                     GPGDVNRLGLRFCGLLTGSNSV"
     gene            7487..8383
                     /gene="murB"
                     /locus_tag="VN478_06850"
     CDS             7487..8383
                     /gene="murB"
                     /locus_tag="VN478_06850"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HWQ22429.1"
                     /translation="MDKFRRRVEQRVLTKEPLSSYTSFRIGGPAEYFYTPSGQEDLVC
                     ALRTARALSMPTTVLGNGTNVLASDRGVEGLVVRLMETSLQPLVEKGLVLARAPMPLS
                     SLLDFSMQQDLCGLEYLVSIPGTVGGAIYMNAGSFSQAISTHLAYVEVLDHNLEYSVK
                     RHEDVDFDYRYSEFQESGAIILGAAFDLEHGDGATIRATAARIRSEKHAKQSWEHPNA
                     GSIFTNPPTTYAGKLIEEAGCKGLQVGQAQVSAKHANFIVNLGGATALDVVRLMDIVV
                     QRVYARFNVRLSPEIRLLGDFS"
     gene            8423..9097
                     /locus_tag="VN478_06855"
     CDS             8423..9097
                     /locus_tag="VN478_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22430.1"
                     /translation="MKLLRRMSLLALVVALTYWFAVPVARVQLKADAWEAPLVGQVWR
                     SRPQGLLMFLNRGSVVDRLAGLPVLDVTLEREWPWNVTVGVDLAEPDVVIRQGGRMAV
                     VFLEMGTAYSVAATKESWTVVNVSGFPSSSPAFLSTSIEYAPLCCELMEHRDQLVISS
                     MSLSSSMGLSVRLKDGKTLVFGDSANSASKIERAIAVLAMPAFKGKKAMIDLRFDGQA
                     IIPGTS"
     gene            9094..9828
                     /locus_tag="VN478_06860"
     CDS             9094..9828
                     /locus_tag="VN478_06860"
                     /inference="COORDINATES: protein motif:HMM:NF017734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HWQ22431.1"
                     /translation="MIRRGRSGAFFLAALFIMVAGFGYLLAVQFEPTVNNAYVVPAQN
                     VELVEIMRGLEEENDSAAQRIVDLRRQLGELESKGAATSDEVRVLTEQLGKLREKAGL
                     SEATSPGVVVTLSDSSRTPTLSDDPNYFVIHDGYLRSIVNLMRRYGATAIAINDQRIL
                     GDSPIICAGPIILVNSSRMSPPYVITALGDPQMLADSISQDVFFRVLEMYRDQFEITC
                     SLEKRASLTVPASLDNAAFKFAKGAK"
     gene            9825..10493
                     /locus_tag="VN478_06865"
     CDS             9825..10493
                     /locus_tag="VN478_06865"
                     /inference="COORDINATES: protein motif:HMM:NF017734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HWQ22432.1"
                     /translation="MKRTGRSSRSTALIFALISFFVGFLLPGQARVVREMKQTYQNED
                     PKDLIRVIKDLQQRKQDLLVEETDVSQKVAQYLRSSDDTAAQVVQLQNEVTVLRQRTG
                     VVPLEGPGIRVTIREQSSGASPSMLADEDLLLLLDDLWASGAEVVSLNGIRLTDSTSV
                     YRAGLNVRVGANVTRMPLVIEAIGDSENMSKGLQIPGGVLDLLNLRRIGAIVQKVNLL
                     RISG"
     gene            10513..10854
                     /locus_tag="VN478_06870"
     CDS             10513..10854
                     /locus_tag="VN478_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HWQ22433.1"
                     /translation="MVGFVTVCGLVLGIVIGILRPVHIPAVYSKYVGLALLAGMDSIL
                     GGIKGYLKDTFDATVFFSGFLINSLVAALLGYLGDQLGIDLYLAAVVVFGSRMYQNIA
                     IIRRVLLKKEA"
     gene            10854..12086
                     /gene="ftsA"
                     /locus_tag="VN478_06875"
     CDS             10854..12086
                     /gene="ftsA"
                     /locus_tag="VN478_06875"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HWQ22434.1"
                     /translation="MAGQERIVSVLDIGSSRVKMLIASATGDSLDAILGVGTAPCNSL
                     KRGVVVDMEGVTQAIQTARDEAERMSSYSVSSVIVNVGGTHITGLVNRGLVAISRPGR
                     EIQKDDVDRVWESAKAVLLGPNQEIIHVIPRQYLVDGQEGIKDPLRMSGIRLEFEAFI
                     VTGQSTILDNLRRCVTESGLKVEKLVLEGLASANAVLSSEEKEIGVACVDIGGDTTDL
                     AVFTGGSLSYIAVLPIGGRHVTMDLAMMLKTSLNVAEELKVAYGSVDASRGKDFQDSQ
                     VSYSTASGDGVLATTKSYISDVVTSRIEEIAEAVKLELVHSSYSNMLPAGLVLTGGTA
                     MVSHITDKFSEISGLRARVGQPTVDDVVFSSFKNPSFSTSVGLLEFGIHSDGASVQKK
                     RRAFKGLSGLFKGLDLFD"
     gene            12114..>12533
                     /locus_tag="VN478_06880"
     CDS             12114..>12533
                     /locus_tag="VN478_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020008727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HWQ22435.1"
                     /translation="MDDRLDPWGSSNVSIKVIGVGGGGGNAVNRMVGDFPSGVDFMSV
                     NTDTQVLRYSRAESKIQIGAKVARGMGAGADPEVGRKAAEESREMLKEAIAGSSLLFI
                     TAGMGGGTGTGGSPVVAKVARELGILTIAIVTKPFNFE"
BASE COUNT         2426 a         3385 c         3921 g         2801 t
ORIGIN      
        1 ctcctggagt cccgagcggt cttcgctgtg ttctggcgca gtagtcagct tcatcacgcc
       61 cgattcgcgc gaatgcaggc cgggagctat gttcgtcgcc attgccggga ccaggctgga
      121 tggagcgtct tacgtcgccg gcgctctcga agcaggagca gtctgcattg ttgcagagac
      181 ggaacacccc gaaattcggg gtgtgcctgt cgtggtcgtc tccaactgcc gtgagttcct
      241 gagtctggcc tcgcaggcat tcttcaactt tccggctcgc agaatgaagt tgatcgggat
      301 aactgggacc agtgggaaga cgagcacggc atacatggct catcgtcttc tgcaggggct
      361 tggtataccg tgcgtcatga tcgggaccac cggctatgtc atgcccagcg gcgagttctt
      421 cgctcctgat acgcttcccg ccaccactcc cgaagccttt tcgctccatt ggtatctgaa
      481 ccacgcggtt cagcagggcg ctcaggttgc ggtgcttgaa gtgtcttcgt tcgctctcgc
      541 cttcgacagg gtctatggcg tgcgcttcga ttgcggggtc ttcacgaatt tctcacaaga
      601 ccacatgggg tatcatggca cgatggatgc ctatcttgcg gccaagctgc gcctgttcac
      661 gtcactggat cgggactcgg tggctgtctt gaatgcagac gatccgtcat tcgccgcgtt
      721 cagggacgcg gccggcggct cgcacgtcag gacgttttct cttgagcatg aggctgatct
      781 gcgtggtcag aacgttgttt cgtccagggg aaggaccaca ctacaggtct tgaccccgag
      841 tggggcagct ttcgacgtgg atctctcgat cggccccgcg tttcaggcgt ccaactgtct
      901 ggcagccatc agttcggtct gtgccatcga gccgacctgc gacccgggca gggcggccct
      961 gtctcttgcc gctccgctgt atgtcccggg acgatatgaa cgcatcgact gcggacagcc
     1021 atttgaggtc gtcgtcgact acgcccacac gccggaggaa ttctcggcgc tattcggagc
     1081 agtacgaccg accgtgcgag gagacctgct tgccgtgttt ggctctgtcg gtgccgacga
     1141 ccgtgagaag cgtccaatca tggcacgtct ggcggagaga gtctgccgct ggtcgtttgt
     1201 gaccgtcgag gatcctcgtc atgaagacgc atcgattgcc gtccgcgaca tccagaccgg
     1261 gttcaccacc gatcactata cggtcatcga agacaggagg ctggcgatcc gaagcgccat
     1321 tgcggcggcc aagccaggcg acatggtcct ggtcctcggt cgtggacatg agacggtcat
     1381 gtactatgaa cacgagaatg tcgctttcga tgatcgagac gaggctcgcc tcgcgctgag
     1441 cgcgcttggc tatcgctgca gcgcttgcgg ttgtgactcg gatgaggccc agcaatgacc
     1501 gtgatgtcag gcatgtcgct ggttctcgtt cttgcccttg tcgacctcac tctcttcccg
     1561 ctcctcatca gatggcttca aaaggcacgg ttcactcagc tcatacggga cgagggccca
     1621 gcttcccaca agtccaaagc aggcactccc accgggggcg gccttatgct tgtcctcaac
     1681 atcgccgtgg ccgtcctggt gtacgccatt ctgatagcca gggcgggctt gacaaaggcg
     1741 gatgtggcca cgctcatcct cgtgggcgtc aacctcctgg gtggtttcct tgatgactgg
     1801 acaaaggagc tcaagcatag gaatgatgga ctgcttggct accagaaact cctgatccag
     1861 actgccgctg ccatgctctt cttctatgtg ggatttcctt cgatcaggcc gattctccag
     1921 acaccaggcg gcagggtcct tcttgctggc tggttcttct atgtggtagc tatactctat
     1981 tctgtcggca tggtcaacgc attcaacctc accgatggcc ttgatggcct gctcgccaag
     2041 acgagtcttc ctgcattcat cgtcgctgcc gcggcgtcga tgctgcatcc gtcgggggtg
     2101 gccggcgtgc tgcctttggc ggctgtggcg ttcggcatct cctttctgtg gttcaacggt
     2161 acaagagcat cagtattcat gggcgacaca ggatcactcg cgctcggttc ggtcttcgtg
     2221 gcgtgggctt tcgtgtccgg caaccagatc ccatcgatcc tggttggagg actgttcctc
     2281 gtagaggcgt tgagcgtcat tatccaggta gtcgtcttca aagcttctgg gcgcaccagg
     2341 agggtgtttc tcatggcgcc gattcatcac cactttgaga agctcgggtg ggctgagccg
     2401 aagattgtcg acagattctt tgtcgtaagc atcttgttcg tcattgcagg agcactgacg
     2461 atggtctgga ggtagccagt gatccctttc gagtctgcat ttgtcgtcgg agctctgcca
     2521 agcggattgt atgctgcact ctaccttcat gatcacgggg tagaggtctt cgtctcggag
     2581 tccaagcagc gaagtgctga cgagaagttc gagcttgcgg tgctcctgtt ggagcgggcg
     2641 ggcattccct tcgaattcgg gcagaacacc ccggacatca tgaccaggtt cgatgctgtc
     2701 gtggtctcac ctggcgtgcc acctgaagct ccgatcatgg tagcggccgc acgggcgggc
     2761 aagttcatcg cgggcgagac ggagattgca gcgtacgcgg gtctgtcagt gcttgcggtg
     2821 actggaagca atggcaagag cactactaca gcccttctcg gagcgatcgt cgctcaggaa
     2881 catccgcgtg tggtgacggg aggcaacctg gggactcctg tgtcacagct ccttgtggaa
     2941 cacccatcgg cgggaccggc cgtcctggag gtcagctgtt tccaactcga aaccatccac
     3001 gagtttcatc ccaaggtagc cctcttctca aatctggtac cgaaccatct tgaccgctac
     3061 ggtgccatgg aacggtactt tgccacgaag aagcgccttt tcgagaacat gacggcagat
     3121 gatgctatcg tgctcaactg ggatgatccc gcgcttcgag agctcggtcc gacgctgtgt
     3181 tctcgcgtct tctacttcag cctgggagat ggcgcgtttc ctggcgtgca cctcgccgat
     3241 ggcgtcatcg tcttcaagga tggtgcgact tgtgtagagc tgttcaggga aacggacctc
     3301 cgtgtcccgg gcaggcacaa tgttgcaaac gccatgagcg cggcacttgc tgccttcctg
     3361 tacggtgttt ccccgggctc aatccagagg gcggtagcag gattcaatgg gttgcctcat
     3421 cgactcgaga acgctggaac gtcgcatggc gtgacgtttg tcaatgactc caaagcgaca
     3481 acgccggagt cggtaatgac ggcagtggac gccatgagtg gagcgtatgt ggtgattctg
     3541 ggcggcagct ccaaggacgt gtcgtttgac aagatggcac ggcatctggc tggtgacaag
     3601 aacctggaga tggcaatcgt aatgggtgcg accggatccg aaatcgagga ttcgcttcgc
     3661 agggctggca tgaagagaat tgtccgtgtt gggtcgcttg aggaagcagt gggcaccgga
     3721 gtgcaactcc ttccagcggg cggtacactg ctgttgtctc ccgcttgcgc cagtttcgac
     3781 atgttcaggg actttgaaca acgtggcgac cggttcaagg agatcgtgca gagtgcgttt
     3841 ggcgcgacga tgcgatgaag cgcatactcg ttcttgtcgg cctgttgctt tgcctgggtg
     3901 tactgcagaa ctacagtatt gccgtcagca ttagcgctgc gcgcggccaa ctggtcgctg
     3961 ttacgccgag tgtcgtcaag caaatcgtga tcgtaggcat cggggcgttc gcctactggt
     4021 tctgctcgcg gttcaagtac atgcggtggg cggacatctc agaattcctg acgtaccttc
     4081 tgggcgccct attggtcgcc gtgctgatac cgggcatcgg caggagcgtc aacgggtcca
     4141 gccgatggat cggaaccaac gtgattggtc tgcaaccgtc tcaggcggcg gtcctggttg
     4201 tacctcttct tgctgccaag ctttttagtg gcaagtactt gaaccagtat gaccgtcgat
     4261 actttcgcac atttctgctg ttcgtcctgg cattcaccgg cctggtcgca ctggagcctg
     4321 acttcggaac agctttcgtc attgccggtt cgtcgatcgc aatcttgctg ggcgtagggg
     4381 ctccctttgg ctactgggcc agtcgaacgg ttgttcttgg tgctcttgcg ctggtggccg
     4441 tgaatgtgaa agcttcctgg aagatcagga tgacctcgtt catcaaccca ttggcggcag
     4501 acaaggccaa aggcgttgca tatcagctgg tcaattccat gtttgccgtg gcgagaggcg
     4561 gagtgctcgg acggggcttc atgcggagtg tccagaagtt cgtccacttc ccggctcagg
     4621 agaacgactt cgtgttcgcg atattctcgg aggaattcgg gcttatcggc gtgaccgttg
     4681 tgctcggagt cttcctctgg atagtcttcg aactgttcag gattgcgtcg agaacctcag
     4741 agccgtttgc ccgagtatat gtgatggcat tgggcgtatt cctggccgtg caggcttttg
     4801 tcaacatcgg tgttgtcgtc ggcctgcttc ccacgacagg tgttcctttg cccttcttca
     4861 gtcagggcgg caatcacatc gtgttcgaga tggctgctct gggaatcgcg ggagccattg
     4921 ctcgcagccc ggaggctgtg tgatgcacta cgtcattgcg gggggaggaa ccggggggca
     4981 catctaccct gctgcagcaa ttgctgagcg gctgtcggat agcggacgcg ttactatgct
     5041 ggcacggtcg ggcagcatgg aggaacgcat cttcctctcg caggggtttg atgtcaggac
     5101 agtcgcttct gcgcctctcc tgtacgaccc tggatcgcta tggcggctcg tacgtgcgac
     5161 cgggcgtgga gtcagggcag cgcgacgggt catgacagac gtgcatgccg atgcgtttat
     5221 cggcaccgga ggatacgtat cagtaccggg gattatcgca gcgcacttga gcagagtacc
     5281 ggtgtatctt ctggaacaga atactgtcat gggcagagcg aaccggttgt tctcgcattg
     5341 cgcgcgacgc gtgttcctgg ggtttccggt tgtgggtgcc cgcggtccgc gatttgccct
     5401 cacagggaac cccctccgac gagatgtcta cgtagcgctg acggagtgca ggtcaagcag
     5461 gccgcttcgc aggggtctac tgttcattgg gggcagtggc ggagcggact tcatcaatgg
     5521 cctgttcctg catacgattc gggagctgga cagcaaggaa cgtgacgtcg aggttaccgt
     5581 ggtgacaggg actgatggct atacgggcat tcgcgatgca gtcgacaagc tggccctgcg
     5641 acacgtccag gccaccgtga tagcgtatga agaccacatg gaacggctct atgcgcacac
     5701 tcgggtggcc gtgacaaggg gtggtgcgct ggtgctcacg gagcttgccg tcggagggat
     5761 ctatgccgtc gctatcccgt acccgttctc agtgggcggc caccagtcca agaacgccgc
     5821 gcatctggag aagctcggcc ttggcgttcg cattgaacag agtctgctga agtttgaggg
     5881 ctacctgtcg atcgtggaga aggcactcga tgacgaaagg ccccccgagg tgcctgcgtc
     5941 cagcgttttt ggaggcgacg ccgcagcagc catagcgcgc tcaatacgag aggagtgctc
     6001 cggtgactga cctgctgaga cccgggactg cctttcttct cgtgggcatc tgtggcgcag
     6061 ggatgagctc attggccatg ctgctgcaca gcatcggaat ggacgtccgg ggttcagacg
     6121 tcatgagtga gactgccgag gcggggaggc tgacgtcgct tggaattccg gtaacgtccg
     6181 aaggtgaagc agccagctcg attcgcgccc ccgacgtcat cgtgcgctcg tctgcgatcc
     6241 aaccgggcaa ccccgtactg gtggcagcac agacaaccgg gtgtgtcgtc ctccaccgaa
     6301 cggatgtgct gcgcgctatc gcgtcagact actacctcat agccgtttct ggcacgcacg
     6361 ggaagacgac tacgacgggg atgatcggat acgcccttgc agaacgtgga ttcgctccga
     6421 ccatctgcgt gggagggcac attgctggat ttgaccagat gtttcccctc aagggaacgc
     6481 agcagaggcg gatcggcggt cgcccgatca tggtcctgga aacggacgag tccgatgcgt
     6541 cgtttctgaa gttccatcca gacgttgccg tcatgacgaa tatcgacagg gatcatctcg
     6601 ggacgtatga aggcaagttc tcgaacctcg tcgacgcatt tgcacggttc tcgcggcagt
     6661 gtgctgagaa gggcggtctg actgtcggtt gtggcgacga caaggaggtg gctggaatcg
     6721 tgggaagtct gccacggtat gtcctctatg gccagggggc cgagaaccag gtgcacatgg
     6781 aatatgatct gctcaccaac acggcaaccg tgtccagaga tggcattgaa cagccgttcg
     6841 tcatggaacg gggagatgaa aagagctacc tcaatgctgt ggccgcttgt ctggcatgcg
     6901 aggcggtcgg tctcgccctg ccagacgcac tacatgtcct ggagcggttt cctgggatgg
     6961 aacggcggat gcaggtcctt gcagacgatg gtcaaatagc cgtgctcacc gaccatgccg
     7021 accaccccac agagatcaga gccactctac gtgcagtagc ggcaaggtat cccgggagac
     7081 cactgactct cgtgctccag ccgcaccgct tctctcgcgt ggctatgtgc cttgacggat
     7141 atggaccggc tctggcggga acctcgaaga tcgtgctgct tgatatcttc cctgcagggg
     7201 agacggtcga acgtccggca gaactcaatc agagacttcg agagagcgtg gaaagctcgg
     7261 ctggtgcttc gttggcacca cgcacgcccg tggatcagat cctggaggat ctccagatga
     7321 ccaccacccc gggtgacgtg gtgctgttca tgggtcctgg tgatgtgaac aggcttggtc
     7381 tgcggttctg tggcttgctg acgggcagca attcggtata atatagggct gctgagcttc
     7441 tcggcggaca ggcaaaccgg gagaacgact gatggtggat tcggtcttgg acaaattcag
     7501 acgtcgcgtc gaacagcggg tcttgacgaa ggagccgctc tcgtcctaca cgtcatttcg
     7561 tatcggaggt ccggccgagt atttctacac gccatcgggt caggaggacc tcgtgtgcgc
     7621 actgcgcaca gccagagctc tcagcatgcc aacgacagtt cttggcaatg gaacgaacgt
     7681 tctagcgtct gaccgcggtg tcgagggcct ggtcgtgagg cttatggaaa cctcccttca
     7741 gccactggtg gagaaggggt tggtcctggc tcgtgcacca atgccacttt caagtcttct
     7801 ggacttctcg atgcagcagg atctgtgtgg actggagtac cttgtcagca tacctggcac
     7861 tgttggtgga gctatctaca tgaatgccgg ttcgttttcg caggccatca gcactcactt
     7921 ggcatatgtg gaggttcttg accacaacct ggagtacagc gtcaagagac atgaagatgt
     7981 ggattttgac tatcgctaca gcgagtttca ggagagcgga gccatcattc tgggtgcggc
     8041 gtttgacctt gagcacgggg atggagctac aatccgggca acagccgctc gcatccggtc
     8101 tgagaagcat gccaagcagt cgtgggagca cccgaacgcg gggagtatct tcacgaatcc
     8161 cccgacgacg tatgctggga agctgataga ggaagctggg tgcaagggtc tccaagtggg
     8221 tcaggctcag gtgtcagcca aacatgcgaa cttcatcgtc aacctgggag gggcgacggc
     8281 acttgacgtc gtgcgcctca tggacatcgt cgtacagaga gtctacgcac gattcaacgt
     8341 acgattgtcc ccggaaatca ggctgcttgg tgacttctca taggaggccg cgtgtgacga
     8401 ccggagacgg gttcctcacc tcatgaagct gctgcggcga atgagtctgc ttgccttagt
     8461 ggtagcgctg acgtactggt tcgccgtgcc ggttgctcgt gtgcaactga aggctgatgc
     8521 ctgggaggcg cctctggtag gccaggtctg gagaagtcga cctcagggcc ttctcatgtt
     8581 tctcaacaga ggttcagtag ttgacaggtt ggcagggctt cccgttcttg acgttacgct
     8641 cgagcgcgag tggccttgga acgttacggt tggtgtggac ctggctgagc ccgacgtggt
     8701 tatccggcaa gggggccgga tggcagtcgt gtttctcgag atggggacgg cttactcggt
     8761 tgcggcgacc aaggagtcgt ggacggtggt caacgtaagc ggattcccat cttcttctcc
     8821 agcgtttctg tctacctcga ttgagtatgc accactctgc tgcgagttga tggaacaccg
     8881 ggaccagctg gtcatctcga gcatgtctct gtcatcctcg atgggactgt cggtgaggct
     8941 taaggatggc aagacactgg tcttcggaga cagtgcaaac tccgcgtcca agattgaacg
     9001 cgctattgcg gtcctggcca tgcctgcatt caaggggaag aaggcgatga tcgacttgcg
     9061 gttcgacggt caggctatta tcccggggac ttcatgatca ggcgcggacg aagcggtgca
     9121 ttcttccttg ccgccctgtt cataatggtt gccggattcg gatatcttct cgcggtccag
     9181 ttcgaaccga cagtgaacaa cgcctacgtc gttcccgccc agaatgtcga gctggtggag
     9241 ataatgcggg gactggaaga ggagaacgac agtgccgccc agcggattgt tgacctgcgg
     9301 cggcagctgg gagagcttga gagcaaaggc gctgccacga gcgacgaggt gcgcgtgctt
     9361 acagagcaac tgggcaagct gagggagaag gcgggcctct ccgaagccac cagtcctggc
     9421 gtcgtcgtta cgttgagcga cagttcgcgt actccgacgc tatcagatga tccgaactac
     9481 ttcgtcattc atgacggcta cctgcgcagc atagtgaatc tcatgcgcag gtatggcgcc
     9541 actgcgattg ccatcaatga ccagcgcata cttggggaca gtcctatcat ctgtgcgggc
     9601 ccgatcatcc tcgtgaactc gagcaggatg tctccgccct acgtcatcac tgcgctggga
     9661 gacccccaaa tgcttgccga cagcatctct caggatgtct tcttccgcgt gctggagatg
     9721 tatcgtgacc agttcgagat cacttgctca cttgagaaga gggcgtctct gaccgtcccg
     9781 gcgtcgctcg acaacgcggc attcaagttt gcgaaggggg ccaaatgaag cggactgggc
     9841 ggtcgtcccg gtcgacagca ctgatcttcg ccctcatcag tttcttcgtc gggttcttgc
     9901 tccctggaca agcacgcgtc gtccgtgaga tgaagcagac gtaccagaac gaggacccga
     9961 aggatctcat tcgtgtcatc aaggacctcc agcagcgcaa acaggacctg cttgtcgagg
    10021 agacggacgt gtcgcagaag gtcgcgcagt acctgcgttc atctgacgat actgctgcac
    10081 aagtggttca gctgcagaac gaggttaccg tgttgaggca gaggacagga gttgtacctc
    10141 tcgaagggcc tggcatccga gtcaccataa gggagcagtc gtcaggagcc tcaccgtcca
    10201 tgctggccga tgaagacctc ctgctccttc ttgacgacct gtgggcttcc ggagccgagg
    10261 tcgtgtcact caacggcata cgactgacag actcgacttc tgtctatcga gctgggctca
    10321 acgttcgagt gggagcgaat gtgacacgca tgcccctggt catagaggcg atcggcgaca
    10381 gcgagaacat gtccaagggc ctgcagattc ccggaggcgt gctcgacttg ttgaacctga
    10441 ggcgcatcgg tgctatagtt caaaaagtca acctgctcag aatcagcggc taggggagga
    10501 aaacggatac ctatggttgg gttcgtgaca gtgtgtggac tcgtacttgg catcgtcatc
    10561 ggaattctcc gtcctgtgca catccccgcg gtctactcga aatacgtcgg gcttgctctt
    10621 ctggctggca tggattcgat cctcggcggc atcaagggct atctgaagga caccttcgac
    10681 gcgacggttt tcttctcggg tttccttatc aacagtcttg tcgcggcgct tctgggatat
    10741 cttggtgacc agctgggcat tgatctctac cttgctgcag tcgttgtgtt tggaagcaga
    10801 atgtatcaga atattgcaat cattcggcgc gttcttctga agaaggaggc atgatggctg
    10861 gccaggaaag aatcgtcagc gttctcgata ttggaagcag tcgggtcaag atgttgatag
    10921 cgtcggcgac aggggacagc cttgacgcca ttcttggggt cggcacagcc ccgtgcaaca
    10981 gcctgaagcg cggtgtcgtc gtggatatgg aaggcgtgac gcaggccatc cagactgcgc
    11041 gcgacgaagc ggaacgcatg agcagttact cagtgtcgtc cgtcatcgtg aacgtaggtg
    11101 gcactcatat taccgggttg gtgaaccgtg gccttgttgc aatatcgagg ccggggcgcg
    11161 agatacagaa ggatgacgta gaccgcgtct gggaatctgc caaagccgta ctactgggac
    11221 cgaaccagga gattatccac gtgatccccc gtcaatatct cgtggacggc caggagggga
    11281 tcaaagaccc gttgaggatg tctggtattc gtctggagtt cgaggcattc attgtcacgg
    11341 ggcagtcgac cattctggat aatcttcgac gctgtgtgac agagtcgggt ctcaaggtgg
    11401 agaagcttgt gctcgaaggc ttggcatcag ccaatgctgt cctgtcgtcg gaggaaaagg
    11461 aaatcggtgt tgcctgtgtc gacatcggag gagacaccac ggacctggcg gtgttcaccg
    11521 gaggcagtct gagctacatt gcagttctgc ccatcggtgg ccgccatgtg actatggatc
    11581 tggcgatgat gctcaagacc agcttgaacg tcgctgagga gctcaaggtg gcctacggtt
    11641 ctgtggacgc gagcagggga aaggactttc aggatagcca ggtcagctac tcgacagcgt
    11701 caggcgacgg ggttctggcg acgacgaagt catacatcag cgacgtggtg acgtctcgca
    11761 tcgaggagat agcggaggcc gtaaaactgg aactggtgca ctccagctat agcaacatgc
    11821 ttccggccgg actcgtcctg accgggggta cggccatggt gtcgcacatc acagacaagt
    11881 tctcagaaat cagtggactg cgcgcccgcg tgggacagcc cacagttgat gacgttgtgt
    11941 tcagctcgtt caagaacccc agcttctcga cctcggtcgg gttgcttgag tttggtattc
    12001 acagcgatgg cgcctcggtt cagaagaaga gacgtgcatt caagggcctg agtggactgt
    12061 tcaagggcct tgacctgttc gactaagaca cactgacggg agggaaatcc gttatggacg
    12121 atcgactgga tccctgggga agctcaaatg taagcatcaa ggttatcggc gtgggcggcg
    12181 gcggcggcaa tgctgtcaat cgaatggtgg gcgactttcc atccggcgtc gacttcatga
    12241 gcgtgaacac cgacacgcag gttctgcggt atagtcgggc agagagcaag atccagatcg
    12301 gggccaaggt cgcccgagga atgggcgctg gcgcagaccc cgaggtcggg agaaaggctg
    12361 cggaagagag cagagagatg ctcaaagagg caattgccgg atcctctctc ctcttcatta
    12421 cggccggcat ggggggcgga acggggaccg gagggtcgcc cgtagtagcg aaagtggctc
    12481 gtgaactggg catcctcact attgcgattg tcaccaagcc cttcaacttt gaa
//
LOCUS       DATMZD010000330         2299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_130669,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000330 DATMZD010000000
VERSION     DATMZD010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130669"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="VN478_06885"
     CDS             complement(<1..647)
                     /locus_tag="VN478_06885"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HWQ22436.1"
                     /translation="MDIDINITISGEAGQGVKTLGSLVSRVLFDRGYYVVQDESYHSR
                     IRGGLDATTIRASDVPHHAVRPLIDLFVPLASSVLAYHAAANRHESEIGPSTRTLCDS
                     KSGVDLPGATRVPATERAIALAGEARAANVFLFGAICRMLGIPLEAATAVTRKVLPSA
                     ILEKNLPVLEDGYRQPVMEVWSLPTRKLAHQVLANASQSIGAAAIVAGVRFYSGYP"
     gene            complement(658..1674)
                     /locus_tag="VN478_06890"
     CDS             complement(658..1674)
                     /locus_tag="VN478_06890"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HWQ22437.1"
                     /translation="MKDLFSELDVAGVRLANRIVLPPMATEQADADGYASTVAASHYG
                     SLAATGVALVVVEHSCVTMEGRASPGQMSLASDDSVAGHAAIAAAIHAGGALCSAQIS
                     HAGSNRRAGMPGRCLGPSAVEHPVSHLIPDEMSGSDVALVVRAFGAAAGRVRDAGYDF
                     VEVHCAHGYLLGEFLSPLTNRRSDAYGGTPAGRRRLLLEVIEEVRAAVHGDLPIMVRL
                     GVADNPPLLQLYEGGLTVAEGVEAARALDGAGVAILDLSAGVCGSRPAGVAGEAYFRP
                     FAEAVSAAVHCHVVCTGGITQPQTGQEVIQSGAADLVGVGRAIAADHDWVHKAREATD
                     HRMA"
     gene            1844..>2299
                     /locus_tag="VN478_06895"
     CDS             1844..>2299
                     /locus_tag="VN478_06895"
                     /inference="COORDINATES: protein motif:HMM:NF036998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HWQ22438.1"
                     /translation="MDERRGQAYHRRFSPLYTDYYEYSMTQGYYLSGKAKDEAVFDIF
                     FRQNPYGGGYAIAAGLRDAVDYVLNMRFTSDELAYLGSLGYRDDFLHVLENTRFTGDI
                     TAVPEGEVIFPGEPIISVKGPLIETQLLEGLILSTVNFQTLIATKARRMV"
BASE COUNT          424 a          719 c          739 g          417 t
ORIGIN      
        1 ggatagccgg agtagaatct gacgcctgcc acgatagcgg ctgcaccgat cgactgactc
       61 gcgttggcaa ggacctggtg ggcgagtttc cgcgtcggca gcgaccacac ttccatgacc
      121 ggctgtctat acccgtcttc gagaacgggc aggttcttct cgaggatggc ggacggcagt
      181 accttgcgcg tcaccgcggt cgccgcctcg aggggaatgc ccagcatgcg gcagatggct
      241 ccgaacagga agacgttggc cgcgcgcgcc tcgcccgcga gagcgattgc ccgttctgtg
      301 gccggaacgc gcgtcgcccc gggaaggtcg acgccggatt tgctgtcaca cagcgttcgc
      361 gtcgaaggcc cgatctcgga ttcgtgtcga ttcgcggcag cgtggtaggc aagaacggag
      421 gacgctaggg ggacaaacag gtcgatgagc ggccggacgg catggtgcgg cacgtcggat
      481 gcgcggatgg tcgtcgcgtc cagcccaccg cggatgcggc tgtggtagct ttcgtcctga
      541 actacgtagt acccgcggtc gaagaggacg cgagagacaa gcgaccccag ggtcttgacg
      601 ccctgccctg cctcgccgct gatggtgatg ttgatgtcga tgtccatgac agctccttca
      661 ggccatacgg tggtctgtcg cttcccgggc cttgtgcacc cagtcgtggt cggcggcgat
      721 ggcacgtccg acgcccacga gatcagccgc tccgctctgg atgacttcct ggccggtctg
      781 tggctgcgtg atgccgccgg tgcagacgac gtgacagtgg acggctgccg agacggcttc
      841 ggcaaacggc cggaaatacg cttcgcctgc aactccggca ggtcggcttc cgcagacgcc
      901 ggcagacagg tcgaggatgg ccacgcccgc accgtcgagg gcacgtgcgg cttcgacgcc
      961 ttcggcaacg gtgagcccgc cctcgtagag ctgcaggaga ggcgggttgt ccgcaacgcc
     1021 cagcctgacc atgatcggaa ggtccccgtg tacagctgcc cgtacttctt cgatgacttc
     1081 cagtagcagg cgcctccgcc ctgcgggtgt cccaccatag gcgtcggatc ggcggttggt
     1141 cagagggctg aggaactcac ccagcagata gccgtgtgca cagtggacct cgacgaaatc
     1201 atatcctgca tcgcggactc ggccggcggc cgctccaaag gcgcggacga ccagggcaac
     1261 atcgctgccg gacatctcgt ccgggatcag gtgagagacc gggtgctcga ccgcgctggg
     1321 accgagacac cttcccggca tccctgcccg ccggttcgac ccggcatggc ttatctgagc
     1381 actgcacagg gcaccgccgg cgtggatcgc cgccgcgatg gcggcgtgtc cagcaacgct
     1441 gtcgtcggat gccagcgaca tctggcccgg cgaggcacgc ccttccatgg tgacgcacga
     1501 gtgttccacg acgaccaggg ccacgcccgt ggcggccagc gatccatagt gacttgccgc
     1561 caccgtactc gcgtatccat cggcgtccgc ctgctcggtt gccatgggtg ggaggacaat
     1621 ccggtttgcg aggcgaacac cagccacgtc cagttcggag aagaggtcct tcatcgtcat
     1681 cctcctgtgc aggatccaga gactcttgat acggagtcat tctagaatct gcgtcaacct
     1741 ttgcaaggat ggtgtctgca ttggacagtg gccgcaagag cacattgagg atatacttga
     1801 ctgtactcaa cgagcggcat cgtgtgttga aaggagcatg accatggatg aacgacgcgg
     1861 acaggcgtat caccgcagat tctcgccact gtacacggac tactacgagt actccatgac
     1921 gcagggctac tatctctccg gcaaggcgaa ggacgaggcc gtgttcgaca tcttcttccg
     1981 ccagaatccg tacggaggag ggtacgccat agctgcaggc ctgcgcgacg ctgtcgacta
     2041 cgtgctcaac atgcgcttca cgtcggatga gcttgcatac ctggggtctc tcgggtatcg
     2101 tgacgacttc cttcacgtgc ttgagaacac gcgcttcaca ggggacatca cagccgtgcc
     2161 ggaaggcgag gtcatctttc ccggcgagcc catcatcagc gtcaagggtc ctctcatcga
     2221 gacgcagttg ctggaagggc tgatcctcag cacagtgaac ttccagacgc tgatcgcaac
     2281 caaggctcgt cgcatggtc
//
LOCUS       DATMZD010000331         3246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_120033,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000331 DATMZD010000000
VERSION     DATMZD010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120033"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VN478_06900"
     CDS             complement(<1..192)
                     /locus_tag="VN478_06900"
                     /inference="COORDINATES: protein motif:HMM:NF021388.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 family protein"
                     /protein_id="HWQ22439.1"
                     /translation="MKRLPTTCPSCGSQLEISELHCPACGTTVRGAYPLDRFAALSAE
                     EEQFLLTFLAARGNLKEVQD"
     gene            complement(200..595)
                     /locus_tag="VN478_06905"
     CDS             complement(200..595)
                     /locus_tag="VN478_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22440.1"
                     /translation="MSQEHDKIESMVQQGKLTRSEADELLAALEPNEPQESRPRTQAV
                     RPHARRTFRIHVNEVGGDNVDLGFPMALAGVGLNILARRGKATIDVDGRQVPIDIEEI
                     KRLIADPDFVGELMNVHTSDGSTVVLSVE"
     gene            complement(623..751)
                     /locus_tag="VN478_06910"
     CDS             complement(623..751)
                     /locus_tag="VN478_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22441.1"
                     /translation="MTDTTTRLMDMVNNGRLDKTTAAMLFNVVWTVSGKREYIIKD"
     gene            1291..2010
                     /locus_tag="VN478_06915"
     CDS             1291..2010
                     /locus_tag="VN478_06915"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HWQ22442.1"
                     /translation="MSEEEMMAVPDTVGSSLWLSLVDMLVVFAVLAFLMVVCYVLRFL
                     TRGSLAEVSIRDSEEAQVDVAAGDEPASEVPMMRSASHATEDSGRLPAAPAMVTSGYT
                     ALATQPRVYKMTVNGKMFSVGIEQMNDVQSGSPEAAVSAPITQVPGTAAPASPVAARS
                     AAAHSPGVSSNLMKSPLPGKILKVFAARGKAFARGDTLIVIEAMKMENEILAPRDCVV
                     AEVDVDVNQSVKTGEPLLRME"
     gene            2015..3187
                     /locus_tag="VN478_06920"
     CDS             2015..3187
                     /locus_tag="VN478_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007952806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HWQ22443.1"
                     /translation="MKVILAAIEGFKSFSGIYNFTLGNAVMLVVACVLMYLAIAKDFE
                     PILLLPIAFGCFLANIPAARGAFFSINPEYVRDLTGTVKPTALLDFFYFLVKSEILPP
                     LIFLGIGAMTDFGPLLANPVSLLLGAAAQFGVFVAMVVAKLLNFTIGQAGAIGIIGGA
                     DGPTAIYTATKLSPELLGPIAVAAYTYMSLVPLIQPPIMRLLSTHEMRSTVMEQLRPV
                     GKLERILFPIVTTFVISILLPPVGPLVGMLMLGNLLRESGVVERLTSTAANELINICT
                     IFLSVAVGASMNATSFLNLKTLYIIGLGVVAFGFSTAGGMLLGDLMWVLSGHKINPLI
                     GSAGVSAVPMAARVSQKVGQQDNPANFLLMHAMGPNVAGVIGTAVAAGIMISLLGH"
BASE COUNT          595 a          957 c          983 g          711 t
ORIGIN      
        1 gtcctggact tccttgagat tgccacgggc agcgagaaac gtcagcagga actgttcttc
       61 ttcggcggat agcgctgcga accggtcgag cggatatgcg ccgcgtaccg tcgtgccgca
      121 ggcggggcag tgcagctcgg agatctccag ctgtgacccg catgacgggc atgtcgtcgg
      181 aaggcgcttc atggcagcct cactcgacac tcagcacgac agtcgaaccg tcgctggtat
      241 gaacgttcat cagctcaccc acgaagtcag gatccgcgat gagacgcttg atttcctcga
      301 tgtcaatggg gacctgcctg ccgtcgacgt cgatcgttgc ctttcctctg cgggcaagga
      361 tgttcaggcc gacacctgcg agcgccatgg gaaagccgag gtcgacattg tcaccgccga
      421 cctcattgac gtggatgcgg aacgtcctgc gtgcatgcgg ccgcacggcc tgcgtccgcg
      481 ggcgggactc ttgcggttca ttcggctcca gtgctgcaag cagctcatct gcctcactgc
      541 gtgtcagctt gccctgctgc accatgcttt cgatcttgtc gtgttcctgt gacatcatat
      601 cctcccggta ttggatgtgt gttcagtctt tgatgatgta ttcgcgcttg ccgctgaccg
      661 tccagacgac gttgaagagc atcgccgccg ttgtcttgtc gagccggccg ttgttcacca
      721 tgtccatcag tctcgtcgtc gtatcggtca tctcgtacct cctggtagga ttctgctcac
      781 ctgcacctaa ataatattaa ggtgcagatg aatgtcaaga gacaaaccgc aagaaggtga
      841 agaaagagtg aagacaagaa atgataggag aatattggcg gttggatgag acgtgcgctg
      901 atgaagcgct cccgtgtatg ggatatatta ttaagggcga agtccagcac gtgcccggag
      961 cctcagatcg accgcccgtc ctgccagtcg gcatgttgtg gaagtgtggc gctgccgggg
     1021 cggttgtgtg tcaagaggga gtgttgccgg tcgcctttgg ggtgacctag acggatggga
     1081 cctttgccgg ctggcgacag acgacccccc ttgatccttc ggcctcgctc tcgagcgcgt
     1141 tctcggtccg gcttccacca gcagggagta ttgcctactt gaccaatttg ttttagaatg
     1201 tggtataatc aattgcacta gttggatggt cgcaggcttg tctgccggcc gatcgggcga
     1261 agccggagcc tccggggccc gaagcaggaa gtgagcgaag aggagatgat ggcggtgccg
     1321 gatactgttg gttcgagtct gtggctgtcc ctcgtggaca tgctggtggt gttcgcggtc
     1381 ctggcgttcc ttatggtggt ctgctacgtg ctgaggttcc tcacgcgagg gtcgctggca
     1441 gaggtctcga tcagggactc tgaggaagcc caggtcgacg tcgcggcagg ggacgagcct
     1501 gcctccgagg tcccgatgat gaggtccgca tcccatgcca ccgaagactc gggacggctg
     1561 ccggctgcgc cggccatggt cacctccgga tacacggcct tggcgacaca gccacgggtg
     1621 tacaaaatga cggtcaacgg gaagatgttt agcgtcggga tcgaacagat gaacgatgta
     1681 cagtcgggga gcccggaggc ggcagtctct gcgccaatca cacaggtgcc cggcactgct
     1741 gctccggcct cacccgtggc tgctcgttct gcggctgctc atagccctgg cgtctcgtcg
     1801 aatctcatga agtccccatt gcccggcaag atcctcaagg tgttcgccgc acgaggcaag
     1861 gcgtttgcgc gcggcgacac gctgattgtc atcgaagcca tgaagatgga aaacgagatc
     1921 ctcgctcctc gtgactgtgt ggtcgcggaa gtggacgtcg atgtcaacca gtcggtcaag
     1981 acgggcgaac ctctcctgag gatggagtag acccatgaaa gtcattctgg ctgcaatcga
     2041 gggattcaag agtttctccg gcatctacaa tttcaccctg ggcaacgccg tcatgctggt
     2101 cgtagcctgc gtgctcatgt acctggctat cgccaaggac ttcgaaccga ttctgctgct
     2161 gccgatagcg ttcggctgtt tcctcgccaa catccctgcg gccaggggcg ccttcttcag
     2221 catcaaccct gagtacgtcc gtgacttgac cggcaccgtc aagccgacag cgctgctcga
     2281 cttcttctac ttcctggtca agtcggagat cctgcccccg ctcatcttcc ttggaatcgg
     2341 cgccatgaca gactttggcc cgctgctggc gaacccggtg tcactgcttc tgggggccgc
     2401 agcacaattt ggcgtgtttg tcgccatggt tgttgccaag ctgctgaact tcacgatcgg
     2461 ccaggcgggc gccatcggca tcatcggagg agcggacggg ccgacggcca tctacacggc
     2521 cacgaaactc tcgcctgagt tgcttggccc aatcgccgtc gccgcatata cgtacatgtc
     2581 gctcgtaccg ctgatccagc cgcctatcat gcgcctttta tcgacacacg agatgcgcag
     2641 cacggtcatg gaacagctgc gtccggtcgg caagctagag cgcatcctgt tcccgatcgt
     2701 cacgaccttt gtcatctcca tcctgctccc gcccgtgggt cctctggtgg gcatgctgat
     2761 gctgggtaac ctgctgcgag aaagcggtgt cgtcgagcgg ctcaccagca ctgctgccaa
     2821 cgagctcatc aacatctgca cgatcttcct ctcggtcgca gtgggggcgt ccatgaatgc
     2881 cacgtcgttc ttgaacctca agacgctcta catcattgga ctgggcgtag tcgccttcgg
     2941 attcagcacc gcaggaggaa tgcttctggg ggacctcatg tgggtgctgt ccggacacaa
     3001 gatcaacccg ctcattggct cagccggcgt gtcggctgtc ccaatggctg cgcgcgtgag
     3061 ccagaaggtc ggtcaacagg acaaccctgc caacttcctg ctcatgcacg cgatgggacc
     3121 caatgtagcc ggcgtcatcg gcactgctgt cgcggcgggt atcatgatca gcctgttggg
     3181 tcactagcga gaacggctca tcgaagagag gccggcagag cgcttctgcc ggcctctctg
     3241 tcgttc
//
LOCUS       DATMZD010000332         2649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_69852,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000332 DATMZD010000000
VERSION     DATMZD010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69852"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            280..792
                     /locus_tag="VN478_06925"
     CDS             280..792
                     /locus_tag="VN478_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22444.1"
                     /translation="MDTLVVYYSRSGSTRTVAAALARGVGGTLRELIDANARTSLFSA
                     AMAALFRRSARLVNPDYDVSGYETIVIMTPIWVGNPTPAANTFLRTADLTGKKVMIVA
                     LGESGENLATSSRLEQMARARGAEVIGVRHIHGLNSSGKAGPSATAEELQNQAQLLAD
                     ALREPMDQES"
     gene            1050..>2649
                     /locus_tag="VN478_06930"
     CDS             1050..>2649
                     /locus_tag="VN478_06930"
                     /inference="COORDINATES: protein motif:HMM:NF019314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWQ22445.1"
                     /translation="MGKDSFIALVSNVSLLVAATVFVHELVGARLLRLDKVVYRVLVG
                     LGLSAITIAVMMIPWVLEPGIVFDTRSVLIGVSGLFFGAVPTVIVMAVASAYRLSLGG
                     AATVMGVCVIVSSGLIGLTWRHMLRRPLTSLTWRDLLALGYAVHIVMLAFAFTLPLQT
                     ALHVLSHIALPVLTIYPVGTAAIGALMVNRLQYEGSIESLRQSEIQFRALSEQAAIGV
                     TKTETVSGRYIFVNQRFADIVGYTREELLAMDFHELTEPEDLADDLDNVDRLIKGRQS
                     EYSMEKRYRRKDGTTIWVNLTVSPLWKAGESPQYLIGLVEDITERKSTERALKQSEDK
                     YRMLTESIKDVVWTLDVETLRFLYVSPSVLGLRGYTPEEVLAQPFEASITPDSAAALQ
                     QAMRAGVDGFVSGREPQGKFFAAEIEQPCKDGSTVWAESVISFYRDDQTSHVCVRGVS
                     RDVSDRRKIASDLAESRADLLEAQAIAHIGDWSMDTTTRAFIWSDELYAIHGVEPGTA
                     MAHVAYAELIHPGDRKRVLETLQSS"
BASE COUNT          502 a          828 c          838 g          481 t
ORIGIN      
        1 gcgctgccac ttactttcca tgcccacacc gtgagggcga attgagacat cgccgtcccg
       61 accagagatg cgacctgccc tgcccacacg acgacgaagg cggccattcc ctgcggctgg
      121 ggcgtatggg tggagctccc agacaatgca ttcatccgac aagtatcata cacgcgaaac
      181 cagcggtcgc aacgccggcg ctccacggca cgccttcttg aacccaaccg gtgtggttct
      241 atactaccgt cagtaacaac ggcgctcggg aggtgtttcg tggacactct ggtcgtgtac
      301 tacagcagaa gtggatcgac aaggaccgta gcggctgccc tggctcgtgg cgtaggtggg
      361 accctgcgcg agctgatcga cgcaaacgcc aggacgagcc tgttttcggc cgcaatggcc
      421 gctctgttcc gacgttcggc ccgcctcgtg aaccccgact acgatgtgtc cggctacgag
      481 actatcgtca tcatgacgcc gatctgggta gggaacccga ctcccgcggc caataccttc
      541 ctgcgtacgg cggacctgac cggcaagaag gtgatgatcg tcgccctggg agagagtggt
      601 gagaacctcg cgacctccag tcgactggag cagatggccc gcgcgcgtgg cgcagaggtc
      661 atcggcgtcc gtcacatcca cgggctcaat tcctcgggga aagcgggacc gtccgccact
      721 gcagaggaac tgcagaacca ggctcagctc ttggcggatg ccctccgtga accaatggac
      781 caggagagct gacgccggcg ctcgcctgaa gaccctcctc attgcgtaag ccggcttgcg
      841 tcctgtctgt gacagtgtcc tggggagtat cccggtacag actggtgccg ctctcgtcgg
      901 cacctgagcg tacgtcgcag gaccagccgt ctggcaagcg ctgacactgt tctgtcatgt
      961 cggtgtcctt gaccaggctg ggccgcagcc tacactggaa gcagcatatg gcgattgtct
     1021 ccagctgtct tgcacggccg aggtgaccaa tgggcaagga ctcgttcata gcgctcgtca
     1081 gtaacgtctc gctgctggtt gccgccacgg tcttcgtcca tgagctcgtc ggcgctcgcc
     1141 tgctcagact ggacaaggtg gtgtaccggg tgctggtcgg actgggactc agcgcgatca
     1201 cgatcgccgt catgatgatc ccgtgggtgc tcgagccggg gatcgtgttc gacacgcggt
     1261 ccgtgctcat cggcgtcagc ggactgttct tcggcgcggt ccccaccgtc atcgtcatgg
     1321 ccgtggcctc ggcgtaccgg ctgtccctgg gcggtgcggc gactgtcatg ggggtctgcg
     1381 tgatcgtgtc ctcgggactc atcggcctca cgtggcgcca tatgctcagg cggcccctga
     1441 ccagcctgac ctggcgcgac ctgctggcac tgggatatgc ggtccacatc gtcatgctgg
     1501 cgtttgcgtt caccctgccg ctgcagacgg cactacacgt gctgagccac atcgcgctgc
     1561 ccgtactgac catctacccc gtgggcacgg ctgccatcgg ggcactcatg gtgaaccgtc
     1621 tccagtatga ggggtccatc gagtcgctga ggcagagcga gatccagttc cgcgcactct
     1681 ccgagcaggc ggccatcggc gtgaccaaga ctgagacagt ctccggcagg tacatcttcg
     1741 tcaaccagcg ctttgcggac atcgtggggt acacgcggga agagcttctg gccatggact
     1801 tccacgaact caccgagccc gaggacctgg cagacgatct ggacaacgtc gatcgtctca
     1861 tcaagggcag acagagcgag tactccatgg agaagcggta ccggcgcaag gacgggacga
     1921 cgatctgggt gaatctcacg gtctctccgc tgtggaaggc cggagagtcc ccgcagtacc
     1981 tcatcggact cgtcgaggac atcactgaac gcaagagtac cgagagagcg ctgaagcaga
     2041 gtgaggacaa gtaccgaatg ctgacggaga gtatcaagga cgtcgtgtgg acgctcgacg
     2101 tcgagactct gcggttcctc tacgtcagcc cttcagtgct ggggctccgc ggatacacgc
     2161 ctgaggaagt actggcccag ccgtttgaag catccatcac tccggacagt gcggcagcgc
     2221 tccagcaggc gatgcgcgcc ggcgtagacg gcttcgtctc gggcagggag ccccagggga
     2281 agttctttgc ggcagagatc gaacagccgt gcaaggacgg gtccaccgtc tgggcagaat
     2341 cagtcataag cttctaccgg gacgaccaga caagtcacgt ctgcgtacgc ggcgtatcac
     2401 gcgacgtcag tgaccgcagg aagatcgcgt ctgatctggc tgagagccgt gccgacctgc
     2461 tggaggcgca ggcaatcgca cacatcggcg actggagcat ggacaccacg acgcgggcgt
     2521 tcatctggtc agacgaactg tatgccatcc acggcgtcga gcccggtacc gccatggcgc
     2581 acgtggcata cgctgagctg atccaccctg gagacaggaa gcgcgtcctc gagacgctgc
     2641 agtccagca
//
LOCUS       DATMZD010000333         3629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_150110,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000333 DATMZD010000000
VERSION     DATMZD010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3629
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150110"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1086
                     /locus_tag="VN478_06935"
     CDS             <1..1086
                     /locus_tag="VN478_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWQ22446.1"
                     /translation="LKLLYRNVSVLILDEPTAVLTPQEIPQLFTELGRLRDKGHTIIF
                     ISHRLDEVLTLSDRVTVMRRGKVVGTSAASELTKEDLARQMVGREVLFMSRREPQEPG
                     DAVFRAQKVNCHDSEHRSRLHDVSIEVRAGEIVGVAGVEGNGQFELVNAIMGLAPMTS
                     GTLTVSGKDITRADILTRRTLIAFVPQDRGKMGASLPASILEDAIMTHHILDPRFHTW
                     RGLGLNYAHAADFTGQIREQFAVSMGHPSDAFSSLSGGNQQKVILGRELLLDTPFVLM
                     DQPTRGLDVGSIEYVHQLLLDLRSRKRAVLLVSADLDELFMVADRIVVLSQGEVAADL
                     ATDATNREEVGYYMLGGKEAAHAETGS"
     gene            1067..2104
                     /locus_tag="VN478_06940"
     CDS             1067..2104
                     /locus_tag="VN478_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWQ22447.1"
                     /translation="MRKQALSGILGVLAAFAIGALVLALQGFNPFATYGALLQYSLFS
                     GVGFTATLTRAVPLVLTGLSASIAFASGPVNLGQPGQLVMGALGATLVGLFVHAPPAL
                     LIPLCILASLLGGALWSGLAALLKRAFAMDEFIVTLMLNFVADYFTLWAITYPLFDPS
                     ASSPMTRPISTAAWLPDMGAINTSVLLMVLGTVFCWLIMSRGRSGYEWRMTGQNSLFA
                     RIGGCKVDANYVRVMLVTGALAGLAGGLMVMGGSHRFVKGLGANYAWDGIMIAVVANN
                     GISGTFLYGLLFSALQQGAIGMELVTAVPSEFIDVLQAVIVLVVVAGRVSLNMVFDRL
                     ATRRKLKESAE"
     gene            2101..2991
                     /locus_tag="VN478_06945"
     CDS             2101..2991
                     /locus_tag="VN478_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWQ22448.1"
                     /translation="MNVLYGLVSAMLVGATPILLAAIGGTFTFYAGIFNIAMEGMMLV
                     GAFCAVIGSFFLHSWFLGILCAIGGALVMAGIFILFAVKLHTDEFLTGIALNLFAVGL
                     TTFLLRDIFHVKGAFTDAGIQAIPSVRLPLIEGIPFVGRLLSGYNLIIYVAVLVTVLS
                     AFVIFRTRFGLRLRGAGYNAACLNSSGVQASTIRIWSLVVCAVCCGLAGAYLSLGSVR
                     LFAENMSAGRGWISLAAVILVSGNPWGIAVISLLFGFFDGLTVFLQGSRIPTQFTAMI
                     PYVATLVSLYFYSVRKRRKA"
     gene            2988..>3629
                     /locus_tag="VN478_06950"
     CDS             2988..>3629
                     /locus_tag="VN478_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HWQ22449.1"
                     /translation="MKTRAWQLERDSRRSAVPLDRRLHPSTWCEQAAGEKGQDDMMFM
                     LWESSVPGSGAPECLYREAAQSMENQGFDESVAVSLIPQGLQLARSGDAPALRVVTAH
                     YLDALFAAPQDPSSPYLGFERPCSWDEVLSRLPAAHTPQEERPDGVEEKTLAGWVGQL
                     AGGAFGTAIEGYTGQRISEVYGEVRSYVTDPETMNDDVVYELALLDAFETHGRN"
BASE COUNT          611 a         1026 c         1169 g          823 t
ORIGIN      
        1 ctcaaattgc tctatcgcaa cgtgtccgtg cttattctgg acgagcctac ggcggttctg
       61 acgccacagg agatcccgca gctgttcacg gaactgggtc gtctcagaga caagggacac
      121 acgatcatct tcatctctca ccggctggac gaggtgctga ctctgagtga tcgcgtgacc
      181 gtcatgcggc gtggcaaggt cgtcggaacg tctgccgcgt cggagttgac aaaggaggat
      241 ctggcccgtc agatggtggg ccgggaggtt ctgttcatgt cccggcgcga gccccaggaa
      301 cccggtgatg ccgtatttcg tgcgcagaag gtcaactgcc atgacagtga gcatcgttcg
      361 aggctgcatg acgtgagcat cgaagtgcgg gctggcgaga tcgttggagt ggccggcgtc
      421 gaaggtaacg gccagttcga gctggtgaac gcgatcatgg gccttgctcc aatgacgtcc
      481 gggacactca cagtaagtgg caaggatatc acccgtgccg acatcctgac gaggcggacg
      541 ctgatcgcgt ttgttcccca ggacagagga aagatgggag ccagtctgcc tgcgtctatc
      601 ctggaggatg cgatcatgac gcatcacatc cttgatccgc ggttccacac gtggagggga
      661 ctcggactca actacgcgca tgccgcggat ttcaccggcc agattcgtga gcagtttgcc
      721 gtcagtatgg ggcacccgtc ggatgcgttc agctcactgt cgggcggcaa ccagcagaaa
      781 gtgattcttg gtcgagagct gctgctggac acgccgttcg tcctcatgga ccagccgacg
      841 cgaggcctgg atgtaggctc catcgagtat gtccatcagc tgctgctgga cttgcgcagc
      901 aggaagcgtg ctgtcctcct ggtgtcggcc gacctcgacg aactgttcat ggtggccgat
      961 cgcattgtgg tcctgagtca gggtgaagtg gccgcagacc tcgccaccga tgcgacgaat
     1021 cgtgaagagg tagggtacta catgcttggc ggaaaggaag ctgcccatgc ggaaacaggc
     1081 tcttagcggg attcttggtg tcctggcagc gttcgcgatc ggcgcgcttg tgctggctct
     1141 ccaggggttc aatccattcg cgacctatgg ggcgctgctc cagtactcat tgtttagcgg
     1201 cgtggggttc accgctactc tgacgcgggc cgttccgctt gtcctgacgg gactctcggc
     1261 ttcgatcgcg tttgcctccg gccccgtgaa cctgggccag ccgggacaac tcgtcatggg
     1321 ggctctcggt gcgaccctgg tcgggctgtt cgtccatgcc cccccggcat tgctgattcc
     1381 gctgtgcatt ctggcatcgc tgctcggcgg tgctctctgg tcggggcttg ctgcgcttct
     1441 caagcgcgct tttgccatgg atgagtttat tgttactctg atgctcaact ttgtcgctga
     1501 ctacttcact ctgtgggcca ttacctaccc attgtttgac ccgtctgcgt ctagtcccat
     1561 gaccaggccg atcagtaccg ccgcctggct gccggacatg ggagccatca acaccagcgt
     1621 gctgctcatg gtacttggga ctgtcttctg ctggctgatc atgagcaggg gacgcagcgg
     1681 ctatgaatgg cggatgactg ggcagaattc tctgtttgcc aggatcggcg ggtgcaaggt
     1741 ggacgccaac tatgtccgcg tcatgctggt gactggagcg cttgcggggc tcgccggcgg
     1801 gctgatggtc atgggtggtt cccatcgctt cgtcaagggg ctgggcgcca actacgcatg
     1861 ggatggcatc atgatcgcag tcgtggccaa caacggcatc tcggggacgt tcctgtacgg
     1921 gctgctcttc agtgcgctgc agcagggggc cataggaatg gagcttgtta ccgccgtgcc
     1981 ttcggagttc atcgacgtgc tgcaggcagt cattgtgctg gtcgtggttg caggcagagt
     2041 ctcgctgaac atggtgttcg accgactggc gaccaggcga aaactcaagg agagtgcgga
     2101 gtgaacgtcc tgtacggact tgtctccgcc atgctcgtcg gggcgacacc gatcctgctt
     2161 gcggccatcg gggggacatt caccttctat gccggcatct tcaacatcgc catggaaggg
     2221 atgatgctag tcggcgcgtt ctgcgcagtc attgggtcct tcttcctgca cagctggttc
     2281 cttggcatcc tgtgtgccat aggtggggcg ctggtcatgg ccggcatctt catcctcttt
     2341 gcggtgaagc ttcacacaga cgagttcttg actgggatcg cgctgaacct gtttgcggtg
     2401 ggcctgacga cgtttctgct gcgggacatc tttcacgtta agggagcatt cactgatgcc
     2461 gggatccagg cgattccatc ggtgaggctg ccgttgatcg agggcattcc ctttgtgggg
     2521 aggctgctca gcgggtacaa cctcatcatc tacgtcgctg tcctcgtgac agtgctttcg
     2581 gcgttcgtca tcttcaggac tcggttcgga ctccgactgc gcggggctgg ctacaatgcg
     2641 gcatgcctca actcgagcgg cgtccaggcg tcgacgatac gcatctggtc tctcgtcgtt
     2701 tgtgcagtgt gctgcggtct ggcaggcgcc tatctctcgc ttgggtcagt acggctgttt
     2761 gccgagaaca tgtccgctgg gcgaggctgg atctcgctcg ctgcggtcat tctcgtcagc
     2821 ggaaacccgt ggggcatagc ggtcatctct ctgctgttcg ggttcttcga cggactgacc
     2881 gtcttcttgc agggcagtcg gattccaacg caattcacgg caatgatccc gtacgtcgcg
     2941 acgctcgtct ccctgtactt ctattctgta cgcaagagga ggaaggcatg aagacccggg
     3001 cgtggcaact agagcgcgat tcgcggcggt ctgctgttcc cctggaccgg cgtctgcatc
     3061 cgagcacctg gtgtgagcag gcggcgggcg agaaggggca ggatgacatg atgttcatgc
     3121 tatgggagag ttctgtgcct ggatcgggtg ctcccgagtg tctctaccgc gaagcagccc
     3181 agtctatgga gaatcagggc tttgacgaga gcgtggcagt ctcgcttatc cctcagggcc
     3241 tgcagctggc acgcagcgga gacgctcctg cactgcgtgt ggtcaccgcc cactacctcg
     3301 acgcgttgtt tgccgcgccg caggatccct caagcccgta tctcgggttc gagcgccctt
     3361 gttcgtggga cgaggtcctg tctcgccttc cagcagctca cacgccgcag gaggagcggc
     3421 ctgacggcgt cgaggagaag acgctggccg gctgggtcgg tcagcttgct ggaggcgcct
     3481 tcggaactgc gatagaggga tacacgggtc agcgcatctc agaagtatat ggtgaggtcc
     3541 ggtcttacgt cacggatccc gagacgatga atgacgatgt tgtctacgag ctggcgctcc
     3601 tcgacgcgtt tgagacacac ggacgtaac
//
LOCUS       DATMZD010000334         2238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_120174,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000334 DATMZD010000000
VERSION     DATMZD010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120174"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..978)
                     /locus_tag="VN478_06955"
     CDS             complement(<1..978)
                     /locus_tag="VN478_06955"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HWQ22450.1"
                     /translation="MRIQTLEVENFMPYRDPVSLDFRDLSLFAIVGETGSGKSSVVDA
                     ICYALYGRIPRIRTENGQRENIISRGAKQFHVALTFDLGGAAYRIVRQGTAMREDVQV
                     FKDGIQLNQLLKKKDADRYIEDTLLHMSFDVFTRIIVLPQGQFDRFLKPDTPRARRDI
                     LISLLSLDIYERIGKAAGTHAGTLMGELGGLDHDLAGIDLSTITDEALNLLDHALSDA
                     GQRVTLVQEQLNARELALAIMEQRMAKTRQLAKLQGDLAALRARTDQVNGDRTRIATG
                     VVLASLQPSLANWAVLATELRKLTDDRDKAASAVVTAQKDLEAAQAAHAD"
     gene            complement(959..2110)
                     /gene="sbcD"
                     /locus_tag="VN478_06960"
     CDS             complement(959..2110)
                     /gene="sbcD"
                     /locus_tag="VN478_06960"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HWQ22451.1"
                     /translation="MRLLHTADWHVGSTLAGLDRFDETGLLLEELFVAAQSEQVDVVV
                     VAGDVFDNPMPGNVSQKLVFDFFARLSGAGIRAVVLAGNHDGQVFVPSKSLLSLGTAS
                     VFEKPSGDQCTVGFRSRADEPVVVTALPYPHERVVMKLNGAGDDVHGDYAASVSTFIR
                     WLAGRMDPSDINVFAAHLMIGGAQLTDTERPMSVSPFFAVPAQSMPSQTMYNALGHVH
                     KHQQIVGAPARTEYSGSIFRVNFSEEGTPKGFNLVDADTKGNLTVRFCELTSAIELHN
                     VECGIDNWARSLEPFRNNGYTKARIRADDPPFNLQGEIRAACPWVVKVEFLHSEAPKT
                     ARPTIEALAGTNLETVQSMYRAYGAGAGEDVMKVLGELYQEVTHADTDA"
BASE COUNT          376 a          700 c          721 g          441 t
ORIGIN      
        1 gtccgcatgg gcggcctgcg cagcctccag atccttctgc gcggtgacga cggcggaggc
       61 ggccttgtcg cggtcgtccg tcagcttcct gagttcggtg gcgaggaccg cccagttggc
      121 cagtgaaggc tgcagcgagg caagcacgac gcccgtggcg atgcgcgtcc ggtcgccgtt
      181 gacctggtcc gtcctggcac gaagggcggc caggtcgccc tggagcttcg ccagctggcg
      241 agtcttggcc atcctctgtt ccattatggc cagggcgagc tcacgggcgt tcagctgctc
      301 ctggaccagc gtgacccgct ggccggcgtc cgagagcgca tggtccagca ggttcagggc
      361 ctcgtcggtg atagtgctca ggtcgatgcc cgccaggtcg tggtccagcc ctcccagctc
      421 acccatcagc gtcccggcat gggtgccggc cgcctttccg atgcgctcat agatgtcgag
      481 gcttagcaga gagatcagga tgtcccggcg cgcgcgcggc gtatcgggtt tgaggaaacg
      541 gtcgaattgc ccctggggca ggacgatgat gcgggtgaag acgtcgaagc tcatgtgcag
      601 gagtgtgtcc tcgatgtatc ggtcggcgtc cttcttcttc aggagctggt tgagctggat
      661 gccgtccttg aagacctgga cgtcctcccg catggcagtc ccctgccgga cgatcctgta
      721 ggcggcgcct ccgaggtcga acgtgagggc gacgtgaaac tgcttggcgc cgcggctgat
      781 gatgttctcg cgctggccgt tctctgtgcg gatgcgcggg atgcgcccgt acaaggcata
      841 acaaatggca tccacgacgg acgacttgcc tgaacccgtc tcacccacga tggcaaacag
      901 cgacaggtca cggaaatcga ggctgaccgg gtcgcggtat ggcatgaagt tctcgacttc
      961 aagcgtctgt atccgcatgg gtcacctcct ggtacagctc gccgagcacc ttcatgacgt
     1021 cctcgccggc gcctgcgccg tatgcgcgat acatcgactg caccgtctcg aggttcgtgc
     1081 cggcaagcgc ctcgatggtg gggcgggccg tcttgggcgc ctcggagtga aggaactcca
     1141 ccttgacgac ccagggacac gccgcccgga tctcgccctg caggttgaac ggcgggtcgt
     1201 cagctcgaat acgcgctttc gtgtatccat tgtttctgaa cggttccagg ctgcgcgccc
     1261 agttgtcgat gccgcactcg acgttgtgca gttcgatcgc cgacgtcagc tcacagaacc
     1321 tgaccgtgag gttgcccttc gtatccgcgt ccacgagatt gaagcccttc ggcgtcccct
     1381 cctcgctgaa gttgacacgg aagatactgc cggagtactc cgtgcgtgcc ggagctccca
     1441 cgatttgctg gtgcttgtgg acgtgaccca gggcgttgta catggtctgc gagggcatgc
     1501 tctgggcggg gacggcgaag aacgggctga ccgacatcgg gcgttccgtg tccgtcagct
     1561 gcgcccctcc gatcatcagg tgcgcggcga agacgttgat gtccgagggg tccatgcgac
     1621 cggccagcca gcggatgaag gtgctgacgc tggctgcgta gtcgccgtgg acgtcgtcgc
     1681 ctgccccgtt gagcttcatg acgacgcgct catggggata cgggagggcc gtcacgacga
     1741 ccggctcatc ggcgcgcgaa cggaacccga cggtacactg gtcacctgag ggcttctcga
     1801 acacggacgc cgttcccagg ctcagaaggc tcttgctggg gacgaatacc tgtccgtcgt
     1861 ggtttccggc caggacgacg gcccggatac ctgcgccact cagccgggca aagaagtcga
     1921 acaccagttt ttggctgacg tttccgggca tcgggttgtc gaagacgtcg cccgcgacga
     1981 cgaccacgtc gacctgttcg gactgagcag ccacaaacaa ctcctcaagc agcagccctg
     2041 tctcgtcgaa cctgtccagc cccgccagcg tgcttccgac atgccagtca gcagtatgca
     2101 gtagtctcat aatgtcagta tagtgccgat tgtcgacgaa cctgtcattc ccaccctagt
     2161 gtctcgggtc aaggcaagga gtgctgccca cgagcatttc tctgcaatgc ccccgagtat
     2221 ttctgttcaa tatgaggc
//
LOCUS       DATMZD010000335         7599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_120180,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000335 DATMZD010000000
VERSION     DATMZD010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7599
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120180"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..1001
                     /locus_tag="VN478_06965"
     CDS             159..1001
                     /locus_tag="VN478_06965"
                     /inference="COORDINATES: protein motif:HMM:NF015509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HWQ22452.1"
                     /translation="MKPSLGLAEMSPVFTLILIVAVGILLRRLHVLSTGDAASIRKFV
                     FNVSLPALTFISLYSNPIPVKALWIIPGVIIIQAVGYVTFRLVPVRYRETIFSGFLGN
                     TGFMGYPVVQAILGQGGLPLAVIYEQTHSFMVFSTWVHRNARNFINPPLVAMLLALVL
                     KRVPLPLFFTDACRLVGNSTSPLAMLFVGLTVDLDFNWRALIAAAVKLALIPAMALAL
                     TLVLPLTGDIRSAFILQSAMPTMVASVVYGAEIGLDVQKLSRNVVVSTLLFPLTILFW
                     ARFL"
     gene            1012..1863
                     /locus_tag="VN478_06970"
     CDS             1012..1863
                     /locus_tag="VN478_06970"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWQ22453.1"
                     /translation="MDRDDLAFVEYALQHPAAGDLIAAVRNSGVSPADLGRACMSAAD
                     ILWADNRLEPALILWEEATRHLDPERQQLQLARCEANMGAGWADRGNAARAAPHAQRG
                     LVLATRLGAWDLVFICASNLGTIAFTREDYPEARRLHSLGLEAAEHTGETAWMAGCLV
                     GLAFDEGAMGDLESARSHLQKALPFARERGDRRLLFTCQRALAHIHLLLRDYPATVQA
                     VLEAISLAEDLGLADAAGELYGELGSAYARQGDIARSQSAYEHSIRAQEAAKQSPPPA
                     GRPDDVQ"
     gene            2222..3118
                     /locus_tag="VN478_06975"
     CDS             2222..3118
                     /locus_tag="VN478_06975"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWQ22454.1"
                     /translation="MGVTIRTARLADRERIVAISSQIWEGDDYVPGVVDEWLRMRDSE
                     VAVADLNGSVIAFARTVSLTPGYVWLEGIRTDVAAQGQGAGRALTEYLIEKATREGAQ
                     RIGLSTYVDNLASIHIIERYHFVRQASFILAETTLDGPARSAARLSPLVSTVPFDEAR
                     AWILGSTSIKTSCGFMSQGWKFWPVARTGVLVWPLFDAAVGIRAADGSLRSLLVVCRP
                     DHGPSETSIDFADGSPEDLDVLVRHALALYPGAKYIETMVPAGQAVHTNLLETVTGLE
                     FDVWRHGEPDVFVYEHEVGQQH"
     gene            3187..4122
                     /locus_tag="VN478_06980"
     CDS             3187..4122
                     /locus_tag="VN478_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017154260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWQ22455.1"
                     /translation="MLVEVNDLTKHFGKLTAVDGVRFSVPAGEAFGLLGPNGAGKTTT
                     VSMMCGLVVPSSGDVIIDGHSVLHDPMAVRRVIGVVPQDIALYPTLSARENLAFFARM
                     QGIPASALASRVDAVLEIVQLADRQKDRIETYSGGMKRRINIAVGLLNQPKLLILDEP
                     TVGVDPQSRNSILDTLQGLNRQGMTLLYTSHYMEEVEFLCTHIAIIDHGKVIADGTQK
                     DLRLQAGNKDVISVQLAREADGALRDRLAELPGVDTVELRDKTLRITTDQGRSLLAGI
                     ISVLGDADCPISTIDVKEPNLEDLFLNLTGTSLRD"
     gene            4124..5332
                     /locus_tag="VN478_06985"
     CDS             4124..5332
                     /locus_tag="VN478_06985"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWQ22456.1"
                     /translation="MKFWHIAVKDLKVRVRDKSAFVVLLILPMAMIIVLGTVFNWAGS
                     SFTARVALVNLDGGDVAGRLAKDVFSSPQLKDLLIVTTVSSESEARTLVEQGKTGAAV
                     IIPAGFSAGVSAGTKVSIVVLGNPETATQAGIIRNVVESFTAEVQRRQLAASIAIQTL
                     QQSGAVPPQHMQEAVQSVVNEISASQQTGVVSVRTATQEGIKVPRALDYYAVGMALLY
                     LIFTANTGTEAMLEEKRQHTLWRVLSTPTSRSQILLGKLVGIFAIAALQFAMIIALTR
                     ALYHVNWGASVPAVIVMAVATVLAGAGLSTLIAALARTPEQAGEIGPAVALIYSLLGG
                     SMWPVYNMPAWLNSLSRLTFTRWSIEGFTSLMVNGGGVSSILRPVGVCLAMAAAFLLI
                     SVTVLNRNYQ"
     gene            5346..6569
                     /locus_tag="VN478_06990"
     CDS             5346..6569
                     /locus_tag="VN478_06990"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWQ22457.1"
                     /translation="MIRRALVIAGLQLRLIARDKSSFIWLLLVPIIFTTLVGIAFGGY
                     WSSGSTPKVPVAFVDLDDSTASSVFGQALRAEPSLDVQTPSETDGRQELKDNRVPVLV
                     IIPRGFGAAVEAGTSASVEVVRSAGQSSGYFLEQLVSNAATRVSTLAYAASVTTDQLS
                     RWVTLDAASRLTAWRTAFADAGTTLADSPTVTTDYSVLARTQPRVQLPDTATQSSTGF
                     GAMFVMAGVLGTATVLVSERLRNTLPRIMTTPTSVMAFLGGKLMAMMVTGVAQFALFI
                     LWGSLVLRVDWGRNPPAIAAMVLLYVFAATGLGVLVGSLCKTMAQASVIASFVTYGTS
                     MLAGSWWPIEVTPPFMQQLARAFPQYWAVNGLNKIVVRGLGFAAIAQNLLVLAIAGTV
                     FLLAGSVIYVRTSRS"
     gene            6580..7254
                     /locus_tag="VN478_06995"
     CDS             6580..7254
                     /locus_tag="VN478_06995"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HWQ22458.1"
                     /translation="MENHGEARQQVAALCHNLVEKGYLTGTGGNVSVRVRGEDAFAIT
                     PSNYDYTIMTPQDVCVLRYDLTVVEGERKPSIESALHAAIYENRPDTNCVIHTHQLYA
                     SAFAITKTPIPALYDEQARFLGRSVEIIDYAPSGTGFLKRNVTVKLRNHCNAYILQNH
                     GALCLGPDPDRTMFNVQLLEKCAVTYLLAICTEKKISRIPLPFREVAFAMLRKDQKKI
                     ENADKH"
     gene            7273..>7599
                     /locus_tag="VN478_07000"
     CDS             7273..>7599
                     /locus_tag="VN478_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HWQ22459.1"
                     /translation="MNEGQTYAISQWHDTAAIYDRLHALIQVPPMNIRPDRMKEFLDY
                     FDTKCTKSRALTDEAKNYIPGGVQHNLAFNYPFPIAVEKADGAFMWDADGNQYIDFLQ
                     AGGPTVL"
BASE COUNT         1443 a         2448 c         2265 g         1443 t
ORIGIN      
        1 ctcctggacc actacctcgt ggtcgcacat cgcatcgcgc agggattccg cgacagggtg
       61 cgacgaggcg aggtgggctg acgtccgcag cactcccgca gagtgtccgg cgtccgaggc
      121 tggacgcaag gctccttttc cactatcatg gcagcgatat gaaaccatca ctcggtcttg
      181 cagaaatgag tcctgttttc accctgatcc ttatcgtcgc tgtgggtatc ctgcttcgca
      241 ggctccatgt cctttcgacc ggagatgcgg ccagcatccg caagttcgtt ttcaacgtct
      301 ccctgcccgc tctcacattc atcagcctgt acagcaaccc catcccggtg aaggcgctat
      361 ggatcatccc gggcgtgatc atcatccagg ccgtcggata cgtgactttc aggcttgtcc
      421 ccgtgcgcta ccgcgagaca atcttctcgg ggttcctcgg gaacaccgga ttcatgggat
      481 atcccgttgt ccaggccatc ctcggccagg ggggactccc tcttgccgtc atctacgaac
      541 agacgcattc gttcatggtc ttcagcacat gggtgcacag gaatgcccgc aacttcatca
      601 acccgcctct ggtcgccatg ctccttgcac tagtcctgaa gcgcgttccc cttcccctgt
      661 tcttcaccga cgcgtgcagg ctggtcggca actcgacgtc gccccttgcc atgctgttcg
      721 taggcttgac tgtcgatctg gacttcaact ggcgggcgct catcgcagct gcggtcaagc
      781 ttgcgctgat accggcaatg gcgctggcgc tgacgctcgt cctcccgctc accggcgaca
      841 ttcgctccgc attcatcctg cagagcgcga tgcccaccat ggtcgccagt gtcgtctacg
      901 gcgccgagat cggcctggat gtccagaagc tgtcccggaa cgtggtcgtc tcgaccctcc
      961 tgttccctct caccatcctg ttctgggcac gcttcctcta gggtacgctc catggacagg
     1021 gacgacctgg cattcgtcga gtatgcgctg caacatccag ccgccggcga tctgatcgct
     1081 gccgtccgca actcgggcgt ttcgccggct gacctgggca gagcctgcat gtccgcggca
     1141 gacatcctct gggccgacaa ccggctggag ccggcactga tcctctggga ggaggccaca
     1201 cgtcatctcg atcccgaacg ccagcagctc caacttgccc gatgcgaggc aaacatgggt
     1261 gcaggatggg ctgatcgcgg caatgcggcc agagcagcac cccacgcaca acgcggactg
     1321 gtgctggcga ccaggctggg agcatgggac cttgtcttca tctgcgcctc caaccttggg
     1381 accatcgcct tcacccgcga ggactacccg gaagcccgtc gcctgcatag tcttggcctg
     1441 gaggcagcag agcacacggg cgagacagcg tggatggccg gctgcctggt cggactggcc
     1501 ttcgacgagg gagccatggg agacctcgag tcggctcgga gtcaccttca gaaagctctg
     1561 ccgtttgcgc gcgagagggg agatcgacgg ctcctgttca cttgtcagcg ggcactcgcc
     1621 cacatccacc tactgcttcg ggactaccct gctaccgtac aggcagtgct cgaggcaatc
     1681 tcgctggcag aggacctggg tcttgccgac gctgcaggag agctgtacgg cgaactgggg
     1741 agcgcgtatg ccaggcaagg cgacatcgcc cggtctcagt ccgcctacga acactccatc
     1801 agggcccagg aagcagcgaa gcagtcgccg ccgccagctg gccgacctga cgacgtgcag
     1861 tagcccccgg cggttccgga tgaagcatca cgaatgctga agcctgcctc ctcccggcat
     1921 tccttttgcc accggcgcag tctgcacctg gaagacgtat cctgcttgta tgcctggcat
     1981 gcccaatctc ggaacacatc ttcaccaggc gggtcgcagt cgacggtatc ggggactgcg
     2041 gaaccccagt gctcatgatc gtctcgaaca ctgaaaccac gatccggatc gttcactgga
     2101 ggagcgtgct cgccgacctg gcggcatccg gcggcggacc attgaacgca gaggtctacg
     2161 gcaatatact tcgtgcagga cagctcagtc tgccgtcact ctgcaatgac aggaggaacg
     2221 tatgggagtc acaattcgca cggcgaggct tgcagaccgt gagcggatcg ttgccatcag
     2281 ttcccagata tgggaaggcg acgactacgt cccaggggtt gtcgacgagt ggctccgaat
     2341 gcgggactcc gaggtggcag tcgccgatct gaacggatcg gtcattgcat tcgcgcgcac
     2401 ggtcagtctg actcccggct atgtgtggct cgagggcatt cgcaccgacg tcgcagctca
     2461 gggacagggt gccggcaggg cactcacgga gtacctgatc gagaaggcga cacgtgaggg
     2521 cgcacaacgc atcggactct cgacgtatgt cgacaatctc gcaagtatcc atatcatcga
     2581 gcgatatcac ttcgttcgtc aggcatcgtt catccttgca gagacgacac tggacggccc
     2641 cgcacggtcc gccgcccgcc tgtcgccact ggtctcgaca gtgccctttg acgaggcgcg
     2701 cgcctggatc ctggggtcaa catccatcaa gacaagctgt ggcttcatgt cacagggctg
     2761 gaagttctgg ccggtagccc ggaccggcgt cctcgtgtgg ccgctgttcg acgctgccgt
     2821 cggcatccgt gcagcagacg gctcgctgcg cagcctcctg gtcgtctgtc gtcctgatca
     2881 tggtccctcc gagacatcga tcgacttcgc ggatggaagc cctgaagact tggacgtcct
     2941 cgtccgtcat gccctggccc tctatccagg agcgaagtat atcgagacga tggtccccgc
     3001 cggtcaggcg gtccacacaa acctgctcga gaccgtgaca ggactcgagt tcgacgtgtg
     3061 gcgccacggc gagccagatg tcttcgttta cgagcacgag gtcgggcaac agcactagcc
     3121 gaccggcatt gccggtacac ccatacctgt actgcagact cagccactta cgacaaggag
     3181 actatcatgc tggtggaagt caacgatctg acaaagcatt ttggcaagct gaccgccgtg
     3241 gatggcgtac gcttcagcgt tcccgctggt gaagcgttcg gcctgctggg acccaacggt
     3301 gccggcaaga ccacgactgt ctccatgatg tgcggcctgg tcgtgccctc aagtggagac
     3361 gtcatcatcg acgggcacag tgtgctccac gacccgatgg cggtgcgtcg tgtcatcggc
     3421 gtcgtgccgc aggacatcgc actctacccg acgctgtcag cccgggagaa cctggcattc
     3481 tttgcgcgca tgcagggcat tccggcgagt gcgctcgcct cccgcgtcga cgccgtcctc
     3541 gaaatcgtcc agctggccga ccgacagaag gaccggatcg agacgtactc aggtggcatg
     3601 aagcgccgca tcaacattgc cgtaggcctg ctcaatcagc caaaactgct gattctcgac
     3661 gagccgacgg tcggcgtgga cccgcaatcg cgcaacagca tcctggacac gctgcaggga
     3721 ctcaaccgcc aggggatgac gctgctgtac acgagccact acatggaaga agtcgagttc
     3781 ctctgcacgc acatcgcgat catcgaccac ggcaaggtca tcgccgacgg gacgcagaag
     3841 gacctccgac tccaggccgg gaacaaggac gtcatcagcg tacagcttgc ccgggaggca
     3901 gatggtgcac tccgcgaccg acttgcagag ctcccgggag tcgacacggt cgaactccgt
     3961 gacaagacgc tccgcatcac taccgaccag ggccgctcac tgcttgccgg catcatctcg
     4021 gtgctcggcg acgcagactg ccccatttcc accatcgacg tgaaggagcc gaaccttgaa
     4081 gacctgttcc tcaatctcac aggaaccagc ctgagggact gacgtgaagt tctggcacat
     4141 tgcagtcaag gacctcaagg tccgtgtccg tgacaagtca gcatttgtcg tcctgctgat
     4201 cctgcctatg gcgatgatca ttgtcctcgg gaccgtcttc aactgggcag gcagctcatt
     4261 cactgcgaga gtcgccctcg tgaacctgga tggcggcgac gtggccgggc gcctggccaa
     4321 agacgtgttc agcagcccac agctgaagga tctgctcatc gtcacgaccg tctccagcga
     4381 gtcagaggcc cgtacgctcg tcgaacaggg aaagaccggt gccgcagtca tcatcccggc
     4441 aggcttcagc gccggcgtga gtgccggcac caaggtgtcc atcgtcgtgc tcggcaatcc
     4501 cgagaccgcc acccaggcag gcatcatccg caacgtcgtg gaatccttca cggctgaggt
     4561 ccagcgcaga cagctggctg catccattgc catccaaacg ctgcagcagt caggggcggt
     4621 cccgccgcag catatgcagg aggccgtcca gtccgtggtg aacgagatca gtgcatcgca
     4681 gcagacgggt gtcgtgtccg tgaggacggc gacacaggag ggcatcaagg ttccccgcgc
     4741 tctcgactac tacgcagtag gcatggctct gctctacctg atcttcactg ccaacaccgg
     4801 caccgaggca atgctggaag agaaacgaca gcacacgctg tggcgcgtgc tgtcgacacc
     4861 gacctcccgc agccagatcc tgctcggcaa gctggtcggc atctttgcca tcgccgcctt
     4921 gcagtttgcc atgatcatcg ctctgaccag ggcgctgtat cacgtcaact ggggcgcctc
     4981 ggtgcctgcg gtcatcgtca tggccgtggc gaccgtgctt gcgggcgcag gtctctccac
     5041 gctgatcgcg gctcttgccc gcactcccga acaggcgggt gagatagggc cggccgtggc
     5101 actcatctac agccttctgg gtggttcgat gtggcccgtc tacaacatgc cggcatggct
     5161 gaactccttg tcgcgactca catttacacg gtggtcgatc gaaggcttca ccagcctcat
     5221 ggtgaacggt ggcggcgtat catccatcct ccgccccgtc ggcgtgtgtc tcgccatggc
     5281 cgcagcgttc ctgctcatct ccgtgacggt cctgaacagg aactaccagt agacaggagt
     5341 ctcccatgat ccgaagagca cttgtcatcg caggtcttca gctccgtctc atcgcacggg
     5401 acaagtcgtc gttcatctgg ctgctgctgg tcccgatcat cttcacgact ctcgtcggca
     5461 tcgcgttcgg aggatactgg tcgtcgggct ccactcccaa ggtgccggtg gcctttgtcg
     5521 acctcgacga ctcgacggcc agcagcgtgt tcgggcaagc cctccgcgcc gagccgtcgc
     5581 tggacgtcca gacaccctcc gagacagacg gcaggcagga gctgaaggac aacagggtcc
     5641 cggtcctcgt cattatcccc aggggattcg gcgctgccgt ggaagccggc accagcgcga
     5701 gtgtcgaggt cgtgcggtcc gccgggcagt cgtccggcta cttcctggag cagctcgtgt
     5761 cgaacgcggc cacgcgcgtg tcaaccctgg catacgcagc gtcggtgaca accgaccagc
     5821 tttcgcgctg ggtcaccctc gacgctgcca gtcgtctcac ggcgtggcgg acagcattcg
     5881 ccgacgcagg cacgacgctg gccgattcac cgacggtgac gaccgactac tccgtgctgg
     5941 cccgtacaca gccgcgcgtc cagctccctg atactgccac ccagagcagt acggggttcg
     6001 gggcaatgtt tgtgatggcc ggcgtcctgg ggacagcgac cgtcctggtg agcgaacggc
     6061 taaggaacac ccttcctcgc atcatgacga cgcccacgtc tgtcatggcc ttccttggag
     6121 gcaaactgat ggcgatgatg gtcaccggag tcgcgcagtt cgcgctgttc atcctgtggg
     6181 ggagtctggt cctgagggtg gattggggac gcaacccgcc ggccatagca gcaatggtcc
     6241 tcctgtacgt attcgccgcc accggtctcg gcgtcctcgt cgggtcgctg tgcaagacta
     6301 tggctcaggc cagcgtcatc gcctccttcg tgacctacgg aacgtccatg cttgcgggtt
     6361 cgtggtggcc gatcgaagtc actcctccgt tcatgcagca gctggcgcgt gcatttccac
     6421 agtactgggc agtgaacggc ctgaacaaga tcgtcgtccg gggactgggg ttcgccgcga
     6481 tcgcgcagaa cttgctggtc ctggcaattg cgggcaccgt gttccttctc gcgggttccg
     6541 tgatctacgt tcgcacatcc cggtcttagg agagcaggca tggagaacca tggagaggcg
     6601 aggcaacagg tagcggctct gtgccacaac ctcgtcgaaa agggatacct gacgggaact
     6661 ggggggaacg tttcagtacg tgttcgcggc gaggatgcct tcgccatcac cccctccaac
     6721 tacgactaca cgatcatgac gccgcaagac gtctgcgtgc tccgctacga tctcaccgtc
     6781 gtggagggag agcgcaaacc ttcgatcgag agcgctttgc acgccgccat ttacgagaac
     6841 cgcccggaca ccaactgtgt gatccatacg catcagctct acgcgagtgc ctttgccatc
     6901 acgaagacgc cgattccggc attgtatgac gagcaggccc gtttcctcgg caggtcggtc
     6961 gaaatcatcg actacgcgcc gtcggggaca gggttcctga aacgcaatgt caccgtgaag
     7021 ctcaggaacc actgcaacgc gtatatcctg cagaatcatg gcgcactatg cctgggaccc
     7081 gatccggatc gcacgatgtt caacgtgcag ctgctggaaa agtgcgcggt gacgtacctc
     7141 ctcgcgatct gcactgagaa gaagatctcg cgcatcccgc tcccgtttcg cgaggtagcg
     7201 tttgccatgc tgcgcaagga ccagaagaag atcgagaacg ccgacaagca ctagcagaac
     7261 aggggggcga ctgtgaacga aggacagaca tacgcgattt cgcagtggca tgacacggcg
     7321 gcgatctatg atcgcctgca cgcattgatc caggtcccgc cgatgaacat ccggcccgac
     7381 aggatgaaag agttccttga ctacttcgac acgaaatgca cgaagtccag ggcgctgacc
     7441 gacgaggcga agaattacat ccccggcggt gtgcagcaca atctggcgtt caactacccg
     7501 ttccccatcg ccgtggagaa agcggacggc gccttcatgt gggacgccga cggcaatcag
     7561 tacatcgact tcctgcaggc cggcggcccg acggtactg
//
LOCUS       DATMZD010000336         2701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_50161,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000336 DATMZD010000000
VERSION     DATMZD010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50161"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="VN478_07005"
     CDS             <1..380
                     /locus_tag="VN478_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012994408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22460.1"
                     /translation="SNICSNHALNALTAAVYLGSVGESGFRALACENAAKLQKLVAGL
                     EATGCFARVFKQSPVFNEVVMASSLDPEDMRSRLAGVPVFPPFALARGVVPDVQGMPH
                     TYLVCATEMLKDSLLEQVLGALK"
     gene            392..1843
                     /gene="gcvPB"
                     /locus_tag="VN478_07010"
     CDS             392..1843
                     /gene="gcvPB"
                     /locus_tag="VN478_07010"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HWQ22461.1"
                     /translation="MTLYEKSVAGRSAFSFNFEENQTSAEALVPEYALAATKSLPQVS
                     ELDLVRHFTNLARSNYGVDTGFYPLGSCTMKYNPEINERLASDPRLTMRHPLDDPMDN
                     QGVLQMEFELKESLQEITGMDDFTLQPACGAHGELTGMLIIHAYMHDHDARRTKVLIP
                     DSAHGTNPASASMAGFQVVTVPSNERGGVDMDALDKLMDDTVAAIMLTNPNTVGLFEE
                     NITLIAELVHARGGLLYYDGANLNALMGLIRPGDLGFDVVHLNLHKTFSTPHGGGGPG
                     AAPVGVKRFLAEYLPIPVVVCRDGRYLLDGDRPRTIGRMSGFYGNTAVLARAYVYIKM
                     MGSDGLLSASRAAVINANYVKVLLSPYFPPAYDRPVMHETVLSAKGYDKYGVTLLDIA
                     KRLMDYGFHPPTIYFPHFPPHAEEVLMVEPTESESKETMDSYCDALIKISQEAKESPE
                     LLLSAPHDTVVTRVDETYAARHLVTSHRDKKGT"
     gene            1848..>2701
                     /gene="argS"
                     /locus_tag="VN478_07015"
     CDS             1848..>2701
                     /gene="argS"
                     /locus_tag="VN478_07015"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HWQ22462.1"
                     /translation="MLNLRETIDRQVGLALGSLLSAEEAPLAACSLSRPGFLDYTLSL
                     PMKLAKTRHKSPVTIASELQPAVAGMACAGLVEAVAPGYINIQLSNSFVADQLTSMLH
                     MDPAVLLKKQANGQKIEVEFVSANPTGPLHVGNGRGAALGSVLASLLETQGYDVDREY
                     YVNDYGSKMKLFTESIAHYLFPLLGLEHAMPAEGYQGAYVPELAQAIYERKGLELRSM
                     AGDQRLAVVEQNGTELVLASIREALDEMGVSFDTWFSEKYLVENGWNDRVLQAFRDRG
                     VVYEKDGAV"
BASE COUNT          562 a          781 c          806 g          552 t
ORIGIN      
        1 catccaatat ctgctccaac catgcactga acgcattgac ggcggccgtc tatctcggca
       61 gcgtgggtga gtcgggattc agggcactgg cctgcgagaa cgcagcgaag ctgcaaaagc
      121 ttgtcgcggg actcgaggcg accgggtgct tcgcccgcgt tttcaagcaa agcccggtgt
      181 tcaacgaagt ggtcatggcg agttcgctcg accccgagga tatgcgatcg cgtctcgctg
      241 gcgtgccggt gttcccgccg ttcgccctcg cccgtggtgt tgtcccggat gttcagggga
      301 tgccgcacac atatctggtc tgcgcgaccg agatgctcaa ggacagtctc ctcgagcagg
      361 ttctcggcgc attgaagtga ggcggtgaga tatgaccctc tatgagaaga gcgtagctgg
      421 ccggtcagcg ttctcgttca acttcgaaga gaaccagacg tcagctgagg cacttgtccc
      481 cgagtatgcg cttgctgcga caaagtcgtt gccgcaggta agcgaacttg acctcgtgcg
      541 gcatttcacg aacctggcca ggtccaacta cggcgtcgat acaggcttct atccgctggg
      601 atcgtgcacg atgaagtaca accccgagat caatgagcga ttggcctctg atccaaggct
      661 caccatgcgc catccgctcg acgatcccat ggacaaccag ggcgttctgc aaatggagtt
      721 cgagctgaag gagtcactcc aggagatcac cggcatggat gacttcacgc tgcagcccgc
      781 gtgtggtgcc catggtgagc tcaccggcat gctcatcatc catgcctaca tgcatgacca
      841 cgatgccagg cggacgaagg tgctgattcc cgactctgct cacgggacga atcctgcgtc
      901 ggcatcgatg gcagggtttc aggtagtcac cgtaccctcc aacgagcgag gcggcgtcga
      961 catggatgcg ctggacaagc tcatggacga taccgtggct gccatcatgc tgaccaaccc
     1021 caatacagtg ggcttgtttg aggagaacat taccttgatc gccgagctcg tgcacgctcg
     1081 tggaggcctt ctctactacg atggagctaa cctcaacgct ctcatgggcc tcatccgtcc
     1141 gggcgatctg gggttcgacg tggttcatct gaatctccac aagacattct ctacgccgca
     1201 tggcggtgga ggtccaggtg ctgctccagt aggcgtcaag aggtttctcg cagaatactt
     1261 gccgattccg gtcgtcgtgt gcagggacgg ccgatatctg ctggacggcg acagaccgcg
     1321 caccatcggc cgcatgtccg ggttctacgg caatacagcg gtgctcgccc gtgcctacgt
     1381 gtatatcaag atgatgggaa gtgacggact gctgagtgca agccgggcag cagtcatcaa
     1441 cgcgaactat gtcaaagtcc ttctgagccc gtacttccct cctgcatatg acaggcctgt
     1501 gatgcacgag acggttctga gtgccaaggg ctacgacaaa tatggcgtga cgctgcttga
     1561 catcgcaaag cgcctgatgg actacgggtt tcatccgccg acaatctact tcccacactt
     1621 cccaccgcat gcagaagagg tcctgatggt cgagccgacc gaaagcgaga gcaaggagac
     1681 catggactca tactgcgacg cactcatcaa gatcagtcag gaagcgaagg aaagcccgga
     1741 gctcctgctc tctgctccgc acgacactgt tgtcacgcgt gtcgacgaga catatgcggc
     1801 ccgtcacctg gtgacgagtc atcgtgacaa gaaaggaact tgagtaggtg ctgaacctgc
     1861 gtgaaacaat agaccggcag gtcggtctcg cgcttgggag cctcctttct gctgaggagg
     1921 ctcctctggc ggcgtgctcg ctgtctcgcc ccggtttcct cgactacacg ctctcgcttc
     1981 ccatgaaact ggccaagact cgacacaagt cccccgtcac catcgcgagc gaactgcagc
     2041 ctgctgtcgc ggggatggca tgtgcagggc tcgtggaggc ggtcgctccg ggctacatca
     2101 acatccagct gtcgaattcg ttcgtggctg accagcttac ctccatgctt cacatggacc
     2161 ctgccgtcct gctcaagaag caggcaaacg gtcagaagat cgaagtggag ttcgtgagcg
     2221 ccaacccgac cggtcccctg catgtcggca acgggcgggg agcggctctc ggatcagtcc
     2281 tcgcttcact tctggagacc cagggatacg atgtggaccg cgagtactat gtcaacgact
     2341 acggctcgaa gatgaagctg ttcaccgagt cgatcgcgca ctacctcttt cccttgcttg
     2401 ggcttgagca tgcgatgcct gccgaggggt atcaaggggc atatgtgccg gaactggccc
     2461 aggcaatcta tgagcggaag gggttggagc tgcgatccat ggctggagac cagcgtcttg
     2521 ccgtcgtgga acaaaacggg accgaacttg tgctggcatc gatccgcgag gctctggacg
     2581 aaatgggggt ctcgttcgat acatggttct cggagaagta cctcgtcgaa aacggatgga
     2641 acgatcgcgt tcttcaggca tttcgagatc gaggcgtagt gtacgagaag gacggagctg
     2701 t
//
LOCUS       DATMZD010000337         3199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_79744,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000337 DATMZD010000000
VERSION     DATMZD010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79744"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..937)
                     /locus_tag="VN478_07020"
     CDS             complement(<1..937)
                     /locus_tag="VN478_07020"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWQ22463.1"
                     /translation="MNLWKKLAYSFGSVAAGLPENAFTAWALYFYVDKLGFPQQLFAT
                     AMLIYTIWNCINDPLFGYWSDRTHTRWGRRIPYIVLGTLPLALAFYLIWVPPAGLSTS
                     AMFWYYLAVIFAYDGFYTIVIINWTSLYPETFSSTRDRSTVNAWRQAIGILGAMAGLA
                     ATPLIVASLGWKGMGIIFGAITAISVFVSLLGSKENLHATGEPLALGPAIKNTLANKS
                     FLTFVMFNLFVNSVTVLVPQVIPFFAKYVLLISDSQSSLIMAPIFIVAIITQIFWSKV
                     VVRKGVRATAMIACVLFGLTLLPLGLVHTMTQAITC"
     gene            complement(969..2294)
                     /locus_tag="VN478_07025"
     CDS             complement(969..2294)
                     /locus_tag="VN478_07025"
                     /inference="COORDINATES: protein motif:HMM:NF023401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ22464.1"
                     /translation="MKVSIIGGGGTRVPILVGALLDLQEQLALTEISLIDPDTGRLAA
                     IDKVISAIVTQKNSTVEVTHAGTFRECVTGASFVIAAIRVGGDRLRTLDERIPLSMDV
                     LGQETVGAGGFAMAVRTIPVVLDMLRDVREVAPDAWFINLTNPSGIITQAMVSHGGFR
                     NVVGICDAPTSIGILLAYLLKVEPEDLVLDYYGLNHCGFVKGVYALGHDVLPQILSMI
                     DQVPDFEAMTHFSPGFVQRLGRLPNGYIWYYAFKRESLAAVKAARETRGEEVERINAQ
                     LFADLQTTDDPGAVYEAYQGARDDGHLAEEFRTAMNLKGGEGYSAVAMDVLMGLAGKR
                     QVVVPVNVMNRGAIAGLALDDVVEVPSLITERLIRPLAVGPVDADSQQLICQVKLYER
                     SLVDAVVYRDLEALIDALALNPLVPSPAVARDIVLAFKEQEAPYFDSFK"
     gene            2632..>3199
                     /locus_tag="VN478_07030"
     CDS             2632..>3199
                     /locus_tag="VN478_07030"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HWQ22465.1"
                     /translation="MYVIVWAALLGDFANSLTVQQQVLMGVSTAVSGILIWISVRALI
                     VQQRFLVQQENMEALEESRAFVRALMDNLPVAVWLKTLDHRYQAANLMWARYNPARAS
                     WTDRSLSHLLGHTDRELYEPDRAREFEQTDDIVVETGRRWEREYSETHEDRQITFHVI
                     KLPVFDVRGSVVSVAGIGLDVTDQRKEEG"
BASE COUNT          669 a          900 c         1019 g          611 t
ORIGIN      
        1 agcacgtgat agcctgcgtc atcgtgtgga ccaggcccag cggaagcagc gtgagaccga
       61 acaggacgca ggcaatcatg gctgtggcgc gaacgccctt gcggacgacg accttcgacc
      121 agaagatctg ggtgatgatg gcgacgatga agataggggc catgatcaga ctgctctggg
      181 agtccgagat gagcaggaca tacttggcga agaaggggat cacctggggc acgagcacgg
      241 tgaccgagtt cacgaacagg ttgaacataa caaacgtcag gaatgacttg ttcgccagcg
      301 tattcttgat ggcgggtccc agagccagtg gttccccggt ggcatgcagg ttctctttcg
      361 accccagcag cgacacgaag accgaaatgg cagtaatggc gccaaagatg atccccatgc
      421 ctttccaacc gagactggct acgatcagcg gcgtcgcggc cagcccggcc attgcaccca
      481 gaatgcctat ggcctggcgc catgcgttga cagtgctgcg gtccctggtg ctggagaatg
      541 tctcagggta cagcgacgtc cagttgatga tgacgatggt gtagaacccg tcgtacgcga
      601 agatgacggc gagatagtac cagaacatag ctgacgtgga gaggcccgcc ggcgggaccc
      661 agatcaggta gaaagccagt gcgagcggca gcgttcccaa gacgatgtat gggatgcgcc
      721 gtccccatcg cgtatgcgtc cggtccgacc agtagccgaa caggggatcg ttgatacagt
      781 tccagatcgt atagatgagc atagcagtgg cgaacagctg ttgcggaaaa cccagcttgt
      841 ccacgtagaa gtacagtgcc caggccgtga acgcattctc cggcaggcct gccgccaccg
      901 aaccaaagct atatgcaagc ttcttccaca agttcatgct gcctccaggg agttgcggat
      961 gtgtgcggct acttgaagct gtcgaagtag ggagcttcct gttccttgaa ggccaggacg
     1021 atatcccggg ccacggccgg tgaaggcacc agcggattga gtgccagagc atcgatgaga
     1081 gcttcgaggt cccggtacac gaccgcgtct acgagagatc tctcgtagag cttgacctgg
     1141 cagatgagct gttgtgagtc cgcgtcgaca ggtccgactg ccagcggacg gatgaggcgt
     1201 tccgtgatga gtgacggcac ctcgacaacg tcgtcaaggg caagaccggc tattgcgccg
     1261 cggttcatga cgttgacggg gaccacaacc tgcctcttgc ccgcgagccc catcagcacg
     1321 tccatcgcaa ccgcgctata tccttcgccg cccttgagat tcatcgcggt cctgaactcc
     1381 tccgcgaggt ggccgtcatc ccgagcgccc tggtatgcct cgtagacggc cccgggatcg
     1441 tcggtagtct gcagatcggc gaacaactgc gcgttgatgc gctccacttc ctctccacga
     1501 gtctctctgg cagccttgac cgctgcgagc gactcgcgct tgaaggcgta gtaccagatg
     1561 tacccgttgg gcagtctgcc cagccgttgc acgaatcctg gtgagaagtg tgtcattgcc
     1621 tcgaagtcgg ggacctggtc gatcatggag aggatctgcg gcaggacatc gtgacccaga
     1681 gcgtagacac ctttcacaaa accgcagtgg ttcaacccgt agtagtccag gacgaggtcc
     1741 tccggctcga ccttgaggag ataggccagc aggatgccta tggaggttgg cgcgtcacag
     1801 atgccgacga cgttacgaaa gcctccatgg ctgaccatag cctgcgtaat gatgcccgag
     1861 gggttcgtga gattgatgaa ccaggcgtcg ggggcaactt cgcgtacgtc cctgagcatg
     1921 tccaggacga cagggatagt gcgcacggcc atggcaaagc cgccggcgcc gactgtctcc
     1981 tggcccagaa cgtccatcga cagtgggatt cgttcatcca gagtgcgcag tcggtcaccg
     2041 cccacacgta tggcagcgat gacaaaggaa gcccctgtca cacactcgcg gaaggtgccg
     2101 gcgtgagtta cctcgaccgt actgttcttc tgcgtgacaa tcgcagatat gaccttgtct
     2161 atggcggcca gccgtccggt gtcggggtcg atgagcgaga tctcggtaag ggcgagctgt
     2221 tcctgaaggt caagaagagc gcctacgagg attggaactc ttgttccacc tccaccaatg
     2281 atgctcactt tcattgctgc ctccagtcgg cgtgaaccgg cacgccgtgg cgcatccggt
     2341 agagggtatc caacatcatg cctgagtctg cctgcacgat ggcggtcctt ctctccaatg
     2401 ttctagcggg ccgtcttcct ttgtcaagga gaggctccag actgttgacg ccggcatatc
     2461 gtgtttgaca gttcttccat gactgctacg ctcaaagacg tcaagggaga aggtgggcgg
     2521 atgcgtctga ggacttcaac ggtcggcaac agtcatagcg gggcgggggc gacggaaagg
     2581 ccaaaatggt ggttgaggcg tgccaggagc ggcgccacag tggcggcttg tgtgtacgtg
     2641 atcgtctggg ccgccctgct gggcgatttc gctaacagcc tgaccgtgca gcagcaggtg
     2701 ctgatgggtg tctccactgc tgtcagcggc atcctcatct ggatatccgt ccgcgctctc
     2761 atcgtccagc agaggttcct cgtccagcag gagaacatgg aggcgctgga ggagtcacgt
     2821 gctttcgtca gagcgctgat ggacaatctg ccggtggctg tctggctgaa gacgcttgat
     2881 cacaggtacc aggcggcgaa tctgatgtgg gcccggtaca acccggcgag ggcgtcgtgg
     2941 acggaccgat cactgtctca cctgttggga cacacagatc gggagctgta cgagccggac
     3001 cgggcaaggg agttcgaaca gaccgacgac atcgtcgtcg agacggggcg ccgctgggag
     3061 cgcgagtact ctgagaccca cgaggacagg cagatcacgt ttcatgtgat caagttgccg
     3121 gtcttcgacg tcaggggttc cgtggtcagc gtggcaggca tcgggctgga cgtcacggac
     3181 cagcgcaagg aagagggac
//
LOCUS       DATMZD010000338         2807 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U13165 k141_79885,
            whole genome shotgun sequence.
ACCESSION   DATMZD010000338 DATMZD010000000
VERSION     DATMZD010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943760
            Sequence Read Archive: SRR7629282
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:27:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,409
            CDSs (total)                      :: 1,374
            Genes (coding)                    :: 1,372
            CDSs (with protein)               :: 1,372
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79885"
                     /isolate="SMAG_U13165"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="46.61 N 94.16 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="VN478_07035"
     CDS             complement(<1..769)
                     /locus_tag="VN478_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010642698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HWQ22466.1"
                     /translation="MDHGAHQGHDHHAHMIHDFQTRFVVCLILTIPVLLLSEPVQVLL
                     HLGTKLQFAGSTWVLLALSAFIYVYGGTPFLRGFVEELKARTPGMMTLIAMAITVAFF
                     YSVAVSLGLQGMVFYWELATLIDIMLLGHWLEMKSVMGASRALESLARLMPSDAHVVM
                     PDGTVMEHPVEHLKTGDRVLVKPGEKVPVDGRVESGASSVNEAMLTGESRPVDKKPGD
                     AVIGGAINGEGALTVVIEKVGGDTYLAQVIEMVRHAQE"
     gene            1282..1770
                     /locus_tag="VN478_07040"
     CDS             1282..1770
                     /locus_tag="VN478_07040"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HWQ22467.1"
                     /translation="MMMRYDPFEELKRAEEQMDRLFGNFFGEPRRRFSALPAAGKTGS
                     DLAPLGEFSAPAIEVQEDAGKYTVKVDLPGIDKKDIHVKVRENVLAVSAESTSETERG
                     DKKSGNYYSERYYGRYYREVPLGSNVDEEHINATHENGVLTLELPKKALPEASSKEIA
                     VK"
     gene            1894..>2807
                     /gene="pyk"
                     /locus_tag="VN478_07045"
     CDS             1894..>2807
                     /gene="pyk"
                     /locus_tag="VN478_07045"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:TIGR01064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HWQ22468.1"
                     /translation="MPDNLNVKRTKIVATLGPATNSEDMIRVLARRGVDVFRINFSHG
                     TDEERLATISTVKKLRGELDLPLAILQDLQGPKIRLGVFSQGPCEIVTGGVLRLTTQA
                     VDGDAEQVSVDYPRLTSEVQPGELIFINDGLIRLKAREIQGDTIVTDVLKGGTLSDHK
                     GVNFPQSDLHVPSITDKDRHDLLTGLRAGVDYVALSFVRTADDVRELRTLMETHGAHI
                     PIIVKVEKWQAVQNIESILAVANGIMVARGDLGVELPIEEVPIIQKRLIALCNRMGKP
                     VITATQMLNSMVENPSPTRAEVTDVANA"
BASE COUNT          570 a          866 c          839 g          532 t
ORIGIN      
        1 attcctgggc gtggcggacc atctcgatga cctgtgccag gtacgtgtcg ccgccaacct
       61 tctcgataac gacggtcagt gcaccttcgc cgttgatcgc tccgccgatg acggcatccc
      121 ccggcttctt gtccacgggg cgcgactcgc ccgtgagcat cgcttcgttc acgctcgacg
      181 ccccactctc gacgcgaccg tcgacgggca ccttctctcc gggcttgact aggactcgat
      241 ctcccgtctt gagatgctcg accgggtgtt ccatgaccgt cccgtccggc atgacgacat
      301 gcgcgtccga gggcatcagg cgcgccaggc tctcaagagc cctggaggct cccatcaccg
      361 acttcatctc cagccagtga ccaagaagca tgatgtcgat gagtgtcgcc agctcccagt
      421 agaagaccat gccctgcagc cccagtgaga cggcgacgct gtagaagaaa gctacggtga
      481 ttgccatggc aatgagggtc atcatgccag gcgtcctggc cttgagctct tcgacgaacc
      541 ctctcaggaa gggagtacct ccatagacgt agatgaatgc cgacagcgcg agcaggaccc
      601 acgtggagcc ggcgaactgc agcttcgtgc ccaggtgcag cagcacctgc actggttcgg
      661 aaaggagcag gacgggtatg gtcaggatca ggcacaccac aaaccgggtc tggaagtcat
      721 ggatcatgtg ggcgtggtgg tcatggccct ggtgcgcgcc atggtccatg tcacctggag
      781 ccatctcatg accggcatgt gtcatcttgc cgtgcatgct gtggtcctgc gcttcctcgc
      841 gcatcgattg catggcgccc ggcatggcgt gatccatgcg atctcccgtc atgtccccgt
      901 gcatggcggt ggtacgtcct tcctgattcg tcgggggtgt ctcgtccgac aagccgtgat
      961 gcacgtgctc gtgttcctgc atgcgctgcc tccgctcgtc tcgtgaactg agtccagtct
     1021 acgtcaaagg tctccttgct caaacccctc tcccgtggac acgatacgcc tacgtccctc
     1081 ggcggtcctg atccatgcag gccgcaggca gagtgcggtc cgggctcctt caggtctcac
     1141 cactgttgtc cgtttcacca ctctttcaca ctgtagcact ccacgtatgt gactgccaat
     1201 tgtataatgt gtgtgtgacc tgaacaggac caaacgacag tcctggacag gaataatgca
     1261 acctacaagg aggtgtgcag tatgatgatg cgatatgatc cgtttgaaga gttgaagagg
     1321 gctgaagaac agatggatcg cctgtttggc aacttcttcg gtgagccccg tcgtcgtttc
     1381 tccgcgctgc ccgccgcagg caagaccggt tcagacctgg cgccgctggg tgagttcagt
     1441 gctccggcga tcgaggtgca ggaagacgca ggcaagtaca ccgtcaaggt agacctgccc
     1501 ggcatcgaca agaaggacat tcacgtcaag gtcagggaga acgtcctggc cgtctcggct
     1561 gagtccacga gtgagactga gaggggcgac aagaagtctg gcaactacta ctccgagcgc
     1621 tactacggtc gctactaccg cgaggtcccg ctgggttcga acgtcgacga ggagcacatc
     1681 aacgcgaccc acgagaacgg agtgctcaca ctcgagttgc ccaagaaggc gctgcccgag
     1741 gcatcgagca aggagattgc ggtgaagtag gcgcagcgtg tgccagaccc gggccccgaa
     1801 catacgttcg gggcctttcg cacttgtgct cgacagcgtt ggagtatcat gtactggaga
     1861 caggctgtcc gcgtcccggc ggtggaggta cacatgcccg acaacttgaa cgtcaagcgc
     1921 accaagatcg tcgccacgct gggaccggca acgaattcgg aggacatgat ccgggtgctt
     1981 gcccgacgag gcgtcgacgt ctttcggatc aacttctcac acgggacgga cgaagaacgg
     2041 ctggccacca tctccaccgt gaagaagctc cgcggcgaac tggacctgcc gcttgccatc
     2101 ctgcaggacc tgcagggacc gaagatccgt ctgggcgtgt tcagccaggg accgtgcgag
     2161 atcgtcaccg gtggtgtcct ccgtctgacc actcaggcag tggatggcga cgccgagcag
     2221 gtcagcgtgg actaccctcg ccttacctcg gaagtgcagc cgggggaact catcttcatc
     2281 aacgacgggc tgattcgact caaggcacgt gagatccagg gggacacgat cgtcacggac
     2341 gtccttaagg gcgggaccct ctcagaccac aagggagtca actttcccca gtcggacctc
     2401 cacgttcctt cgatcaccga caaggacaga catgacctgc tcacaggact gcgggcggga
     2461 gtcgactacg tcgccctgtc gtttgtgcgc acggcggatg acgttcgaga gcttcgtacg
     2521 ctcatggaaa ctcatggcgc acacatcccc atcatcgtca aggtggagaa gtggcaggcc
     2581 gtgcagaaca tcgagagcat cctcgccgtg gcaaacggca tcatggtcgc ccggggagac
     2641 ctgggcgtcg agttgccgat cgaggaagtc cccatcatcc agaagagact catcgccctg
     2701 tgcaaccgaa tgggcaagcc cgtcatcacc gcgacgcaga tgctcaactc gatggtcgag
     2761 aacccatctc ccacgcgggc cgaggtgaca gacgtagcca acgccat
//
