LOCUS       DATNAE010000001         2009 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2709765, whole genome shotgun sequence.
ACCESSION   DATNAE010000001 DATNAE010000000
VERSION     DATNAE010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2709765"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1190
                     /locus_tag="VN518_00005"
     CDS             <1..1190
                     /locus_tag="VN518_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase subunit alpha"
                     /protein_id="HXE02986.1"
                     /translation="GKPEHVINFFFFVAEEVREYMASLGLRSFAEMIGHTEILDKSQA
                     IDHWKAKGLDFSRIFHKPEVPPEVAIYNCERQDHGLDKVLDVKLIELAREAIEDKKPV
                     KLDLPIKNIDRTTGAMLSGEIATRYGHTGLPEDTIWVSLTGSAGQSFGAWLAHGVTLD
                     LVGEGNDYVGKGLSGGRIIVRPPANSAIVPEKSIIVGNTVLYGAISGECYFRGIAGER
                     FAVRNSGAIAVVEGTGDHGCEYMTGGVVVVIGPTGRNFAAGMSGGIAYVLDEEGDFER
                     RCNLAMVDLEPIPSENQVMERSRHQIGDLESHGLVDFTDMTRFDEERLYQLIENHLHY
                     TGSTRAKAILDDWASYLPKFVKVMPVEYRRALEEMARRQSVDKAGLGEIEIGLRANGG
                     NGK"
     gene            1342..>2009
                     /locus_tag="VN518_00010"
     CDS             1342..>2009
                     /locus_tag="VN518_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016920531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXE02987.1"
                     /translation="MGKITGFLEIEREDRTYEPASDRIRHYREFVIPPSEAEIGRQAA
                     RCMDCGIPYCHTGCPVNNQIPDWNDLVYHADWNVAAQNLHSTNNFPEVTGRICPAPCE
                     AACTLNIIDVPVTIKSIECAIADKAFDEGWVTPLPPETKTGKRVAIVGSGPAGLAAAQ
                     QLARAGHDVHVYEKSAFPGGLLRYGIPDFKMEKYIVERRIKQMEAEGVTFHCNVHVGR
                     DLPV"
BASE COUNT          396 a          648 c          636 g          329 t
ORIGIN      
        1 agggcaagcc cgagcacgtc atcaatttct tcttcttcgt cgccgaggaa gtgcgcgagt
       61 acatggcctc gctcggcttg cgcagcttcg ccgaaatgat cggccacacc gagattctcg
      121 ataagagcca ggcgatcgac cattggaagg cgaagggcct cgatttcagc cgcatcttcc
      181 acaagcccga ggtcccgcct gaggtcgcca tctacaattg cgagcggcag gaccacggcc
      241 tcgacaaggt cctcgacgtc aagctgatcg agcttgcccg cgaggcgatc gaggacaaaa
      301 agccggtcaa gctcgacctg ccgatcaaga atatcgaccg taccaccggc gccatgctgt
      361 cgggcgagat cgccacgcgc tacggccata ccggtctgcc cgaagacacc atctgggtga
      421 gcctgaccgg ctcggccggc cagagcttcg gcgcctggct cgcgcatggc gtgacgctgg
      481 acctggtcgg cgagggcaac gattatgtcg gcaagggcct gtcgggcggc cgcatcatcg
      541 tccggccgcc ggcgaactcg gccatcgtgc cggagaagtc gatcatcgtc ggcaacacgg
      601 tgctgtacgg cgccatctcg ggcgaatgct attttcgcgg tatcgccggc gagcgctttg
      661 ccgtgcgcaa ctcgggcgcc atcgccgtcg tcgaaggcac cggcgaccat ggctgcgaat
      721 atatgactgg cggcgtggtt gtggtgattg ggccgacggg gcgcaatttc gccgccggca
      781 tgtccggcgg catcgcctac gtgctggatg aggaaggcga tttcgagcgt cgctgcaacc
      841 tcgccatggt cgacctcgag ccgatcccga gcgaaaacca agtgatggag cggagccgtc
      901 accagatcgg cgatctcgag agccacggcc tggtcgattt caccgacatg acccgcttcg
      961 acgaggagcg gctctaccag ctgatcgaga atcatctcca ctacaccggc tcgacgcgcg
     1021 ccaaggcgat cctcgacgat tgggcgagct acctgccgaa attcgtcaag gtgatgccgg
     1081 tcgaatatcg ccgcgcccta gaagagatgg cgcgccgtca gtcggtcgac aaagcgggtc
     1141 tcggcgagat cgagatcggc ttgcgcgcca acggcggcaa cggcaaataa tcctcaacgc
     1201 gaaggagcca agatgcctgg agaaaggcgc caggcggcga ctttaccgcg cgagatgggc
     1261 ggtgcttgac ggagcgaggc tgacggtgac tacttggagg accgccaacc ggcgagagtt
     1321 tggaccaaga gcatgggttt gatgggcaag atcacgggct ttctggagat cgaacgcgag
     1381 gaccggactt acgagccggc ctccgatcgc attcgccatt accgcgagtt cgtcatcccg
     1441 ccgagcgagg cggagatcgg ccgccaggcg gcgcgctgca tggattgcgg cattccctat
     1501 tgccacaccg gctgcccggt gaacaaccag atccccgact ggaacgacct cgtctaccac
     1561 gccgactgga acgtggcggc gcagaacctg cactcgacca acaatttccc cgaagtgacc
     1621 ggtcggatct gcccggcgcc gtgcgaggcc gcctgcacgc tgaacatcat cgacgtcccc
     1681 gtcaccatca aatccatcga atgcgccatc gccgacaagg ccttcgacga aggctgggtc
     1741 accccgttgc cgccggagac gaagactggc aagcgcgtgg cgatcgtagg ctccggtccc
     1801 gccgggctcg ccgccgcgca gcagctcgcc cgcgccggcc atgacgtgca tgtctacgag
     1861 aagagcgcct tccccggcgg cctgctccgc tacggcatcc ccgatttcaa gatggagaaa
     1921 tatatcgtcg agcgccgcat caagcagatg gaggccgagg gcgtcacctt ccattgcaac
     1981 gtccatgtcg ggcgcgatct gcccgtcaa
//
LOCUS       DATNAE010000002         3804 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2108103, whole genome shotgun sequence.
ACCESSION   DATNAE010000002 DATNAE010000000
VERSION     DATNAE010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3804
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2108103"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1047
                     /locus_tag="VN518_00015"
     CDS             <1..1047
                     /locus_tag="VN518_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015932430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HXE02988.1"
                     /translation="DKVGGMTRVFGWDLGGANVKLARIDGGRVVHVAQIPCPIIPERR
                     KFDEAVDAALPLISYPALHAVTMTGELSDVFNDRADGVAYLVELMQRVTAGQTLRVYA
                     GSFGFLDPDDAKNRTLEVASANWHATASLVARRHADGLLVDAGTTTTDLIPIKAGKVT
                     ARGATDAERLTEGELLYTGVVRTPVMAVAHSAPFRGRVQKIAAERFATMADVWRLTGD
                     LPDDADPYPAADQRGKSPGESAGRLARMLGRDASEAELAEWMALARYFADCQLAEIEH
                     TARNLAERDTLSPDAPVVGAGCGRFIARQLAERLGRRYLDFGDLIDAPPETREMAARC
                     APAVAVALLAGKGK"
     gene            complement(1051..2109)
                     /locus_tag="VN518_00020"
     CDS             complement(1051..2109)
                     /locus_tag="VN518_00020"
                     /inference="COORDINATES: protein motif:HMM:NF024120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HXE02989.1"
                     /translation="MTFKSLLRSAGCLLGALVTAACFMPLAADAQDAAQRGCVTAPKS
                     PLVVNVKDKGAKGDGRSDDTAAIQTAIDDVGGTGGTVLVPSGTYMVDAVEKKRRLALR
                     SDMTLKLAKDAILKAIPNDSRKYSVLSISGVSNVTLVGGTLEGDRTEHQGKAGEAGMG
                     LRIERGAKDVTVAGVTSRKMWGDGFYVEDAKNTKFCAVTADNNRRQGLSIVEADGVLV
                     TDSIFSNTQGTRPSAGIDLEPDQPEQKIVNIRIQNSKFLNNAGPGIEIAGKRGAVAKV
                     ELSHNMFKGNRPILVENAPDVLASAICGNRQVTSEKASSEGLNAFADPIDIVVHQNDC
                     EDGSDMRFEVNRQTRKKK"
     gene            2227..2811
                     /locus_tag="VN518_00025"
     CDS             2227..2811
                     /locus_tag="VN518_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HXE02990.1"
                     /translation="MSDLDLALSIALGVGLAAATGFRVFLPMLVVSVAAYTGHLSLGE
                     NFAWLATPGALIMLGVAALVEILAYYIPGVDNLLDVLATPAAVVAGTLVSAAVITDLP
                     PMLKWTTAIIAGGGVAGLTQGVTALLRAKSTVLTAGVGNPVIATAELGGSLLVSLLAL
                     AAPLVALLVVIVFLWLAMRLIRRIARSASSSSTG"
     gene            2900..3247
                     /locus_tag="VN518_00030"
     CDS             2900..3247
                     /locus_tag="VN518_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE02991.1"
                     /translation="MRYGLVPTGVLIAVALGTASGASAAPAPGMAWSKAASIVQPVGY
                     WDRQYRRYGPYATPPVVVVPQPAQVVPPVVVEQSVIVVPPPRPASCGEYRYWNGQYCV
                     DARYNRPYLGPRP"
     gene            complement(3255..>3804)
                     /locus_tag="VN518_00035"
     CDS             complement(3255..>3804)
                     /locus_tag="VN518_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF447 domain-containing protein"
                     /protein_id="HXE02992.1"
                     /translation="TTLNAEGKVHIAPLGLIAEEESWILAPFKPSTTLDNLRANPYAV
                     ANFTDDVLVFASCLTDRRDWPTHHATHIPGAILDGTLAHAELKVDYVEEDEQRPRFHC
                     KVVFEAAHALFKGFNRAQGAVIEAAILASRLQMLPREKIERELGYLQIAVEKTAGPRE
                     HEAWRLIVEKIEDYFKHAHEPR"
BASE COUNT          598 a         1229 c         1229 g          748 t
ORIGIN      
        1 gacaaggtgg gcggaatgac acgggtcttt ggctgggatc ttggcggggc caatgtgaag
       61 ctcgcgcgca tcgacggcgg gcgcgtggtt catgtcgcgc aaatcccctg cccgatcatt
      121 cccgagcgcc gcaaattcga tgaagccgtc gatgcggcgc tgccgctgat ttcctacccc
      181 gccctgcatg ccgtcaccat gaccggggaa ttgtccgacg tgttcaacga ccgggcggac
      241 ggtgtcgcct acctggtcga gctgatgcag agggtcacgg cgggtcagac gcttcgcgtc
      301 tatgcaggca gcttcggctt tctcgatcct gacgatgcca agaaccggac gctcgaggtc
      361 gcctcggcca attggcatgc gactgcttcc ttggttgcca ggcgccatgc ggacggactc
      421 ctcgtcgatg ccggcacgac gaccaccgac ctcattccga tcaaagccgg gaaggttacc
      481 gcccgcggcg ccaccgacgc cgagcgcctg accgaaggcg agctgctcta taccggcgtg
      541 gtgcgcacac cggtgatggc ggtggcgcac tcagcgccgt ttaggggccg ggtgcagaag
      601 atcgcggcgg agcgcttcgc caccatggcc gatgtctggc ggctcacggg cgatcttccg
      661 gacgatgcag acccttaccc agcagccgac cagcgcggaa agagtccagg cgaaagcgca
      721 ggccggctcg cccgtatgct cgggcgtgac gcaagcgagg ccgagcttgc cgaatggatg
      781 gcgctcgcgc gctatttcgc cgactgccag cttgccgaga tcgagcatac ggcgcggaat
      841 ctcgccgagc gcgacacgct ttcgccagat gcgccggtgg tgggcgccgg ctgcggccgc
      901 ttcatcgcga ggcagctcgc cgagcggctc ggccgacgct acctcgattt cggggatctc
      961 atcgatgcgc cacccgagac tcgcgagatg gccgcgcgct gcgcccctgc ggtcgccgtc
     1021 gccctgctcg ccggcaaagg caaatgacgc ctacttcttc ttgcgcgtct gccggttgac
     1081 ctcgaagcgc atgtcgctgc catcctcgca gtcgttctgg tggacgacga tgtcgatcgg
     1141 gtcggcgaag gcattcaatc cttccgacga tgccttctct gaggtgacct gccggttgcc
     1201 gcagatggca gaggcgagca cgtcaggcgc attctcgacc aggatggggc gattgccttt
     1261 gaacatgtta tgcgaaagct cgaccttggc gacggcgccc ctcttgccgg cgatctcgat
     1321 cccagggccg gcattgttca ggaacttcga attctgaatg cggatgttga cgatcttttg
     1381 ctccggctga tccggctcga gatcgatgcc ggcgctcggg cgcgtcccct gcgtgttgct
     1441 gaagatcgaa tccgtcacca gcacgccgtc ggcctcgacg attgacagtc cctgccggcg
     1501 gttgttgtcg gcggtcacgg cgcagaattt cgtgttcttg gcgtcttcga catagaaacc
     1561 gtcgccccac atcttgcgtg aggtcacgcc ggcgacggtg acgtccttgg cgccgcgctc
     1621 gatgcggagt cccatgccgg cctcgccagc cttgccttgg tgctcggtgc gatcgccttc
     1681 gagcgttcca ccaaccaagg tgacgttcga cacgccggaa atggagagga cggaatattt
     1741 ccgcgagtcg ttggggatcg ccttgaggat ggcgtccttg gcgagcttca gcgtcatgtc
     1801 gcttcgaagg gcaaggcgcc gcttcttctc aaccgcgtcg accatatagg tgccgcttgg
     1861 cacgagcacg gtgcctccag ttcctccgac gtcgtcgatc gcggtttgaa tagccgcagt
     1921 gtcatcgctg cgcccgtcgc ccttggcgcc cttgtccttg acgttgacga caagcggcga
     1981 ttttggcgcc gtgacgcatc ctcgctgcgc cgcatcttgt gcgtcggcgg caagcggcat
     2041 gaagcacgcc gccgtcacga gtgcgcccag gaggcatcct gccgacctga gtaagctctt
     2101 gaaagtcatg ggtcggcgaa tccctggacc tggaccgttc cgaggcgtaa gcgaatccgg
     2161 cgtcaaggcc gcatcattgt ctgcgacctg ggcattattt ggcccgccgg cgactaagga
     2221 aggcgcatgt ccgaccttga tcttgcactc tcaattgccc tcggcgtcgg gctcgcggcg
     2281 gcgaccggct tccgcgtctt tctgccgatg ctggtggtaa gcgttgcggc ctataccggg
     2341 catctttctc taggcgagaa cttcgcctgg ctcgcaacgc caggggcgct gatcatgctc
     2401 ggcgtggcgg cgctcgttga gattctcgcc tactacattc ccggcgtcga taatctgctc
     2461 gacgtcttgg ccacccccgc cgccgtcgtc gccggcacgc tggtctcggc ggcggtgatc
     2521 accgaccttc cgccgatgct gaaatggaca acggcgatca tcgccggcgg cggcgtggcg
     2581 gggctcacgc agggcgtcac cgctctgctg cgggcgaaat cgacggtgct cacggcgggc
     2641 gtcggcaacc cggtgatcgc cacggcggag cttggcggct ccctcctcgt ctcgcttctt
     2701 gcgcttgcgg ccccgctcgt cgcgctcctc gtggtcatcg tgttcttgtg gctggcgatg
     2761 cggctgatca ggcgcattgc acgaagcgcg agctcatcga gcaccggtta accaaagacc
     2821 gccgctttgg cacacgaggc cttgcgccgg cctacggtta ggctcgaaaa tgagcccaat
     2881 gaaacagggg gagtgtccta tgcggtatgg tctcgtaccc accggcgtgt tgattgccgt
     2941 agcacttggc acggcgagcg gtgcaagcgc cgcgcccgcg ccaggcatgg cctggagcaa
     3001 ggcggcatca atcgtccagc ctgtcggcta ttgggaccgc caataccggc gttatggtcc
     3061 ttacgccact cctcccgtcg ttgtcgttcc ccagccagct caggtcgttc cgcctgtggt
     3121 cgtcgagcag tcggttatcg tcgtgccgcc gccgcgcccg gcaagctgcg gcgaatatcg
     3181 ctactggaac ggccagtact gcgtcgatgc gcgctacaac cggccgtatc tcggcccgag
     3241 gccgtaacga gagatcagcg cggctcgtgc gcgtgcttga aataatcctc gatcttctcg
     3301 acgatcaggc gccaggcctc gtgctcgcgg ggacctgcgg tcttctccac cgcgatctgc
     3361 agatagccga gctcgcgctc gatcttctca cgcggcagca tctgcagccg gctcgctaga
     3421 attgccgcct cgatgaccgc tccttgcgcg cggttgaagc ctttgaacag cgcatgggcg
     3481 gcctcgaaca caactttgca gtggaatcgc ggacgctgct catcctcctc gacatagtca
     3541 accttcaact cggcatgggc gagcgtgccg tcaaggatgg cgccgggaat gtgggtggcg
     3601 tgatgcgtcg gccagtcgcg cctgtcggtc aggcagcttg cgaagacgag gacatcgtcg
     3661 gtgaagttcg ctaccgcgta aggattggcc cgcaaattgt cgagcgtggt cgacggcttg
     3721 aacggcgcga ggatccagct ctcttcctcg gcgatgaggc cgagcggcgc gatatgcacc
     3781 ttgccctcgg cgttgagggt ggtg
//
LOCUS       DATNAE010000003         2215 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_904427, whole genome shotgun sequence.
ACCESSION   DATNAE010000003 DATNAE010000000
VERSION     DATNAE010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_904427"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="VN518_00040"
     CDS             <1..673
                     /locus_tag="VN518_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008548061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXE02993.1"
                     /translation="DAFSKRFPTIRLVEGYGLTEASPVVCCSALRVPAKANSIGQPLP
                     ATDVRFADLDNWRKVVPLGERGELQVKGPQVMLGYYNNQKESDAVFADGYLRTGDVGY
                     MDKDGYVFLVDRIKDLIICSGFNVYPRVIEDALYRHEAVDEVNVIGVPDAYRGEAPVA
                     FVKLKAGASATEAQLKAFLGEHINKLEMPREIIFKDQLPKTLIGKLSKKELRDEYAAM
                     KAKVK"
     gene            670..1065
                     /locus_tag="VN518_00045"
     CDS             670..1065
                     /locus_tag="VN518_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014131685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding protein"
                     /protein_id="HXE02994.1"
                     /translation="MNRATAEPRDLFAIADLAAEFGISTRAIRFYESKGLLAPERVGG
                     ARIFRRRDRARLMLILRGKRLGFSLRDISDYLGLYDAHSQTAQVALLVEKVDERLALL
                     EGQLADLQTTIAELREIRQLARERLDQDR"
     gene            1233..1841
                     /locus_tag="VN518_00050"
     CDS             1233..1841
                     /locus_tag="VN518_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE02995.1"
                     /translation="MAILLLTACTITSERSLTSGDEAATPLPDAFTLFPYQQSANGYI
                     PEAISPMQFVREGSDYVGTDIQESSEPLRVRFVRLNDDLFLLAAMVSDSPAVTYGFAR
                     YNDGVLSIALSPDASTALALRRLRHRMMPKERLPLAGVTVAPGTDAITVRSRAALNDL
                     GRLYAAGRLPLAPPAVGYVSLDSSAPPPSRLVPSAGDWIEVQ"
     gene            complement(1845..2132)
                     /locus_tag="VN518_00055"
     CDS             complement(1845..2132)
                     /locus_tag="VN518_00055"
                     /inference="COORDINATES: protein motif:HMM:NF014232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HXE02996.1"
                     /translation="MSMSGIERKIAELSREMADAVAAEDFERAAALRDEIAVLKGQAG
                     VAEGPVVRQPPPGQMGLGTHVPVVAPPKGWHPPKKPDPMTRNSRPGGRRRR"
BASE COUNT          385 a          741 c          694 g          395 t
ORIGIN      
        1 cgacgccttc tcgaagcgct tcccgacaat ccggctggtc gagggttatg gcctcaccga
       61 agcctcaccg gtggtctgct gctcggcgct gcgcgtgccg gcaaaggcca attccatcgg
      121 ccaacccctg ccggcgacgg atgttcgctt cgccgatctc gacaattgga ggaaggtcgt
      181 tccgctcggt gaacgcggcg agctgcaggt caaggggccg caggtgatgc tgggctatta
      241 caacaatcag aaagaaagcg atgcggtgtt cgccgacggt tatcttcgga ccggcgacgt
      301 cggctacatg gacaaggacg gctacgtctt ccttgtcgac cggatcaagg acctgatcat
      361 ctgctccggt ttcaacgtct atccgcgtgt catcgaggac gcgctctacc ggcacgaggc
      421 ggtggacgag gtcaacgtca tcggcgtgcc cgatgcctac cggggcgagg cgccggtcgc
      481 cttcgtcaag ctcaaggccg gcgcgagcgc gaccgaggcc caactcaaag cgttccttgg
      541 cgagcacatc aacaagctcg aaatgccgcg cgaaatcatc ttcaaagacc aattgcccaa
      601 gacgttgatc ggcaagctct ccaagaagga gctgcgcgac gaatatgcgg cgatgaaggc
      661 caaggtgaaa tgaaccgcgc caccgccgag ccgcgagatc tcttcgccat cgccgatctc
      721 gcggcggaat tcgggatctc gacgcgcgcc atccgcttct acgagagcaa aggactcctt
      781 gcgcccgaac gggtcggcgg cgcgcgcatc ttccggcggc gcgaccgggc gcggctgatg
      841 ctgatcctca ggggtaagcg gctcggcttc tccctgcgcg acatctccga ctatctcggc
      901 ctttacgacg cacactccca gaccgcgcag gtcgcgcttc tggtcgagaa agtcgacgag
      961 cggctggccc tgctcgaagg ccagctcgcc gatcttcaga cgacaatcgc cgagctcagg
     1021 gaaatccggc aactggcgcg tgaaaggctg gatcaggaca ggtgaaccgg agccccagct
     1081 ggagacttat tcagctcgcc ttcatctcgg cctcgctacc cggcgccgag cagtgcctgc
     1141 ggaactgcgg caatgtaagg cgcttcggca ccgtccgccg accttcgagg aggcgatcat
     1201 tgcgtcgacc agtccgcctg atgctggctg cattggcgat cctcctgctc accgcctgca
     1261 cgatcacctc cgagcgatcg ttgacgagcg gcgacgaggc ggcgacaccg ctgcccgatg
     1321 ccttcaccct cttcccttac cagcaaagcg ccaatggcta tattccagag gcgatatcgc
     1381 cgatgcagtt cgtccgcgag ggatcagact atgttggcac ggatattcag gagtcatccg
     1441 agcccctgcg cgtccgcttc gtccggctca acgatgacct cttcctgctt gccgcaatgg
     1501 taagcgacag tcctgcggtc acttatggct ttgcgcgtta caacgacggg gtgctttcga
     1561 tcgcgctgtc gcccgatgcc agcaccgcgc tcgccctgcg gcgcctgcga caccggatga
     1621 tgccgaagga gcggctgccg ctcgccggcg tgaccgtcgc gccgggcacg gacgcgataa
     1681 cggtcaggag tcgcgcggcg ctaaacgatc ttggacggct ttacgccgcc ggacggttgc
     1741 cgctcgcacc gccagccgta ggctatgttt cgctcgactc gagcgccccg ccgccctcgc
     1801 ggctggtccc gagcgccggc gactggatag aggtgcagta gcgctcagcg ccgccgccgc
     1861 ccacccggcc tcgaattgcg ggtcatcggg tcgggctttt tcggaggatg ccagcctttg
     1921 ggcggcgcga cgacgggcac atgggtgccg agacccatct ggccgggcgg tggctgacgg
     1981 acgaccggcc cttcggccac tccggcctgc cctttcagca cggcgatctc gtcccggagc
     2041 gccgcggcac gctcgaaatc ctcggccgct acggcgtcgg ccatctcgcg gctcagctcg
     2101 gcaatcttgc gttcgatgcc gctcatcgac atgtcaggtc gcctccagga atatctgcat
     2161 atcggcaagc gtcgcggctt cggcagcggg cttgtcccag cggatgcgat tgatg
//
LOCUS       DATNAE010000004         3055 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_302936, whole genome shotgun sequence.
ACCESSION   DATNAE010000004 DATNAE010000000
VERSION     DATNAE010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302936"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VN518_00060"
     CDS             <1..252
                     /locus_tag="VN518_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HXE02997.1"
                     /translation="RAIEVQHRSGQILERVNAYFGYRAIAEMRILQAPVARKTPRRHA
                     AEPPVDPDVLPASASLEDGGLRAALLRLGTNTRAKLTSR"
     gene            340..1221
                     /locus_tag="VN518_00065"
     CDS             340..1221
                     /locus_tag="VN518_00065"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HXE02998.1"
                     /translation="MAKSEKKAAVQPGSGRAASGKSSGAETPPGKDEAGNKNTLFIIG
                     AVAAAILVGGLIYWKTSSDGNAGGPGPKTDPDLAALMAPGPLPEMTLGSADAPNTIVE
                     YASMTCPHCAQFETVVFPELKTKYIDTGKVRFIFREFPLDGLAARASLLARCAGPDRY
                     FPMIEALFQTQQNWVVQGPEAMEKLLQFARQAGLSKESFDKCMADKELFNKIVEIRTT
                     GYEKFGIDSTPSFFINGKRMSGEHTLKDFEAKLVGIDKPAETGGSTPPAGSASPTTEG
                     GATPTPQEGKPEGSSGQ"
     gene            1271..1903
                     /locus_tag="VN518_00070"
     CDS             1271..1903
                     /locus_tag="VN518_00070"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HXE02999.1"
                     /translation="MALALSGCGGGAMGPTPPIDKATYVPPQAELMAPGPLGDVSLGN
                     ANAPVTVIEYVSLTCPHCAHFQKALFPRFKKEFIDTGKVRFIVREFPIGHTSGNAALV
                     NRCAPEDKYFFLLNQFLTRQSEWVSQEVRPDAIYSVAKSSGMSRETFDKCLSNQTIID
                     GLTEVKQRGRQFNVIGTPTFFINGRKAQGEVTFDEIKAMIEQAPPPATPS"
     gene            1972..>3055
                     /locus_tag="VN518_00075"
     CDS             1972..>3055
                     /locus_tag="VN518_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003682 - chromatin binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007076 - mitotic chromosome condensation
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation SMC family protein"
                     /protein_id="HXE03000.1"
                     /translation="MKITRLRLLGFKSFVEPTELLVEPGLTGVVGPNGCGKSNLLEAL
                     RWAMGETSYKSMRGAAMEDVIFAGSEDRPGRNSAEVTLFIDNSTRTAPAEFNDSDALE
                     VTRRIEREAGSAYKVNGKDARARDVRLLFEDAATGARSPALVRQGRIGEIVNSAPQER
                     RRVLEDAAGIAGLHSRRHEAELRLKATEANLARLADLIGQLASQLQALRRQARQAQRY
                     REISGKIRETEALQHHLHWTGACAHVEQEEAAFQHALSQVAQHTKAEGEALRQQAEAA
                     EKLQPLRDEEAARAAVLHRLSVERDALEREEARAKARQAELETRLAQASRDLAREEEH
                     IAEAEGMLRGLGEEGRRLAVASSRDRE"
BASE COUNT          583 a         1001 c         1011 g          460 t
ORIGIN      
        1 cgcgccatcg aggtgcagca ccgctcaggg cagatcctcg agcgggtgaa cgcctatttc
       61 ggctaccggg caattgccga gatgcgcatc ttgcaggccc cggttgcgcg caaaacgccg
      121 cgcaggcacg ccgcagagcc gccggtcgat cccgacgtgc tgcccgcctc cgcttccctc
      181 gaggatggcg gcttgcgcgc ggccttgctg cggctcggca ccaacacccg cgccaagctc
      241 acaagccgtt gagcgccttt atctcgctct tgccagaacg cgggtgttta acgaatgtag
      301 gcccggctcg aagcgatccc agcaggtgga gtgaaaagag tggcgaaaag cgagaaaaag
      361 gcggctgtcc agcccggttc cggcagggcg gcgagcggca aatcctcagg cgccgagacg
      421 ccgcccggca aggatgaggc cgggaacaag aatacgttgt tcattatcgg cgcggtggcg
      481 gcggccattc tcgtcggcgg actcatctac tggaagacga gttccgacgg caatgccggc
      541 ggaccaggcc ccaagaccga tcccgacctc gccgccttga tggcgcccgg tccgctgccc
      601 gagatgactc tcggcagcgc cgacgcgccg aacaccatcg tcgaatatgc ctcgatgacc
      661 tgcccgcact gcgcgcagtt cgagacggtc gtgtttcccg agctcaagac caaatacatc
      721 gacaccggca aggtgcgttt catcttccgc gaattcccgc tcgacgggct cgcggcgcgc
      781 gcctcgctgc tcgcccgctg tgcgggcccg gatcgctatt tcccgatgat cgaggcgctg
      841 ttccagacgc agcaaaactg ggtcgtgcag ggccccgaag ccatggaaaa gctgctgcaa
      901 ttcgcgcgtc aggcaggctt atcgaaggag agcttcgaca aatgcatggc ggacaaggag
      961 ctgttcaaca agatcgtcga gatccggacc accggctacg agaaattcgg catcgactcg
     1021 acaccctcct tcttcatcaa cggcaagcgg atgagcggcg agcatacgct caaggatttc
     1081 gaggccaagc tcgtcggcat cgacaagccg gcagaaaccg gcggctcgac cccgcccgca
     1141 gggagtgcct ctccgacgac agaaggcggc gcaacgccga cgccgcaaga aggcaagcct
     1201 gaagggtctt cgggtcagta ggaacttagc aatgcggctg cgagggctaa gagcgtcgat
     1261 tccgatcgcc ttggcgctcg ccctttcggg gtgcggcggc ggcgcgatgg gcccgacgcc
     1321 gccgatcgac aaggcgacct acgtgccgcc acaagccgag ctgatggcgc ccggcccgct
     1381 cggcgacgtc tcgctcggca acgccaatgc gccggtcacc gtgatcgaat atgtctcgct
     1441 cacctgcccg cattgcgccc atttccagaa agcgctgttc ccgcgcttca agaaggaatt
     1501 catcgacacc ggcaaggttc gcttcatcgt gcgggagttc cccatcggcc acacctccgg
     1561 caatgcggcg ctggtcaatc gctgcgcgcc ggaagacaaa tatttcttcc tgctgaacca
     1621 gttcctgacc cggcagagcg aatgggtgag ccaggaagtg cgcccggacg cgatctattc
     1681 ggtcgccaaa tccagcggaa tgagccgcga gacgtttgac aaatgcctat cgaatcaaac
     1741 cattatcgac ggcttgacgg aggtgaagca gcgggggcgg caattcaacg tgatcggcac
     1801 gccgaccttc ttcatcaatg gccgcaaggc gcaaggagag gtcaccttcg acgagatcaa
     1861 ggcgatgatc gagcaggccc cgccgccggc aactccgtct tgagcgttac ccggacaccg
     1921 gcgatggtcc ggtcccgtgc gtagaggctc cactgacaag gtagacacag cgtgaagatc
     1981 actcgtcttc gccttctcgg attcaagtcg ttcgtcgagc cgaccgaact cctggtcgag
     2041 ccgggcctga ccggcgtggt cggccccaac ggctgcggca agtcgaacct gctcgaggcg
     2101 ttgcgctggg ccatgggcga gacctcgtac aagagcatgc gcggcgccgc catggaggac
     2161 gtgatcttcg ccggctcgga agaccgcccg gggcgcaata gcgccgaggt cacgctgttc
     2221 atcgacaaca gcacgcggac cgcgcctgcc gagttcaacg acagcgacgc actcgaggtg
     2281 acgcggcgga tcgaacgcga ggcgggctcg gcctataagg tcaacggcaa ggatgcgcgc
     2341 gcgcgcgacg tgcggctcct gttcgaggat gcggcgaccg gcgcgcgctc cccggcgctg
     2401 gtgcggcagg gccgcatcgg cgagatcgtc aattccgcgc cgcaggaacg ccgccgcgtg
     2461 ctcgaagatg ccgcggggat tgccggcttg catagtcgcc gccacgaggc cgagcttcgc
     2521 ctgaaggcga ccgaagccaa tctcgcgcgg cttgccgatc tgatcggcca gctcgcatcg
     2581 caattgcagg cgttgcgacg ccaggcgcgc caggcgcagc gctaccggga gatctccggc
     2641 aagatccgcg agaccgaggc gctgcagcat catctgcact ggaccggcgc gtgcgcgcat
     2701 gtcgagcagg aggaagcggc gttccagcac gcactctccc aggtcgcgca gcacaccaag
     2761 gccgaaggcg aggcgctgcg ccagcaggcc gaggccgccg agaagcttca gccgctgcgc
     2821 gacgaggagg ccgcgcgggc ggccgtgctg catcgcttga gcgtcgagcg cgatgcgctg
     2881 gagcgcgagg aggctcgcgc caaagcgcgg caggccgagc tcgagacgcg gttggcgcaa
     2941 gcttcgcgcg acctcgctcg cgaagaggag cacatcgccg aggccgaagg catgctgcgc
     3001 gggctcggcg aagagggtcg gcgtcttgcc gtggcgagtt cgcgcgatcg cgagg
//
LOCUS       DATNAE010000005         3263 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2411174, whole genome shotgun sequence.
ACCESSION   DATNAE010000005 DATNAE010000000
VERSION     DATNAE010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2411174"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(206..760)
                     /gene="rsmD"
                     /locus_tag="VN518_00080"
     CDS             complement(206..760)
                     /gene="rsmD"
                     /locus_tag="VN518_00080"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019963140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HXE03001.1"
                     /translation="MRIVAGRFKGASLEAPKGLSTRPTSDRVRQALFNVLEHGPARFE
                     FEGARVLDLFAGTGALALEALSRGARYAVLIEEDAAARAAIRRNVEALSLTGVTKIWR
                     RDATRLGEAGTLQPFDLVFCDPPYGKGLGERALASAATGGWLAKGAIAVLEERAANEI
                     AWPASFQEIDRRRYGDTVIALAQG"
     gene            complement(764..1552)
                     /locus_tag="VN518_00085"
     CDS             complement(764..1552)
                     /locus_tag="VN518_00085"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HXE03002.1"
                     /translation="MAKPTEPMRIAKAIAHAGICSRRDAERMIEAGRVAVNGKVLTTP
                     AHVVSAKDRITVDGKALPEAQSPRLFRYHKPRGLVTSHKDPQGRRTVFEALPEGLPRV
                     VSVGRLDINTEGLLLLTTDGELARHLELPSTGWLRRYRVRVHGRVTEEALDALRQGVT
                     IDGIHYGQVEVRIDREQGANLWLTLGLREGKNREVKRLAEHLGLTVNRLIRVSFGPFA
                     LGDLAEGAVEEVKPKVLADQLGRKLAGDLGLLQAIKERRQAHRG"
     gene            1686..2117
                     /locus_tag="VN518_00090"
     CDS             1686..2117
                     /locus_tag="VN518_00090"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HXE03003.1"
                     /translation="MDLALEEAEAAAARGEVPIGAVVVGASGEVLAKASNRTIELRDP
                     TAHAELLAIRAACSKLGSERLLDCDLYVTLEPCAMCVGAISFARIRRLYFGAADPKGG
                     AVEHGPRFFAQPTCHHAPEVIGGLGEARASALLKSFFAARR"
     gene            complement(2123..2311)
                     /locus_tag="VN518_00095"
     CDS             complement(2123..2311)
                     /locus_tag="VN518_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03004.1"
                     /translation="MSTEGALAHQSAGEQWEQALLSAKRHGTLAGPRPGQVCLWSKPL
                     GACLWYTPSKWNLMLTEH"
     gene            complement(2544..>3263)
                     /locus_tag="VN518_00100"
     CDS             complement(2544..>3263)
                     /locus_tag="VN518_00100"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HXE03005.1"
                     /translation="QVPTFSDATAGALAFSLQILFDFVAYSDMAMGIGLTLGLMLPLN
                     FNTPYRALSVRDFWRRWHITLSRWLRDYIYIPLGGSREGVVVYIWATMVTMGLCGLWH
                     GAGWTFVLWGLGHGMGLLICRLWQHYGIPLPPLLAWAITMVFVILLFVLFRASDISAA
                     GRMVTGLAGGGGLGEAWPWMTVLPIAIAGGLALIKVPSAELTMWLRPTWPVAIGFIVI
                     AVFCVLEVGEGQPLSFIYFQF"
BASE COUNT          593 a         1090 c         1036 g          544 t
ORIGIN      
        1 ggaatcgtag ttggcctcgc ccttccacac ccctgagacg aaggccgtgc cctgcggcga
       61 cccggcaaca ctggtcgcca tgagaaaacc ccccaagagc aaggctctga tggtcatggc
      121 actcctcgca tgccgccgga actccattaa agcacaatgc gggcccgagc gggtgtgttg
      181 tccccttcaa ccatgcgtgt tcggctcagc cctgtgccaa ggcgattacg gtatcgccgt
      241 agcggcgccg gtcgatttcc tggaaggacg cgggccacgc gatctcgtta gcggcccgct
      301 cctcaagcac ggcaatggcg cccttggcca accagccccc ggtggcggcc gaggccaaag
      361 cccgttcgcc gaggcccttg ccataaggcg ggtcgcagaa gacgaggtcg aagggctgaa
      421 gcgttccggc ttcgccgagc cgcgtcgcgt cgcgccgcca gatcttggtg acgccggtca
      481 atgacagcgc ctcgacattg cggcgaatgg ctgcccgcgc cgcggcgtcc tcctcgatga
      541 gaacggcgta gcgggcgccg cgcgacaacg cctcgagcgc gagcgctccg gtgccggcga
      601 acaggtcaag cacgcgcgcc ccctcgaact cgaagcgcgc cggtccgtgc tcgagcacgt
      661 tgaacagcgc ctggcgcacg cggtcggagg tcggacgtgt cgaaagcccc ttgggggcct
      721 cgagcgaagc gcccttgaat ctgccggcga cgatccgcat ggcttaaccg cggtgcgcct
      781 ggcggcgctc cttgatcgcc tgaagaaggc caagatcgcc ggcgagcttc cgcccgagtt
      841 ggtcggcgag caccttaggc ttgacctcct ccacggcgcc ctcggccaag tcgccaagcg
      901 cgaacggccc gaacgagacg cggatcagcc ggttcacggt aaggccgaga tgctctgcta
      961 gccgcttcac ctcgcggttc ttgccttcgc gcaggcccag cgtcagccag agattggccc
     1021 cttgctcgcg gtcgatgcgc acctcgactt gcccataatg gatgccgtcg atggtcacac
     1081 cctggcgcag tgcgtcgagt gcctcctcgg tgacgcggcc atgcacgcgc acccggtagc
     1141 gcctgagcca gcccgtgctc ggcagctcga gatggcgcgc gagctcgccg tcggtggtca
     1201 gcaacagcag cccttcggta ttgatgtcga gccgcccgac cgagacgacg cggggaaggc
     1261 cttcgggcaa agcctcgaac accgtccttc gcccttgcgg gtccttgtgg cttgtcacca
     1321 ggccacgcgg cttgtgatag cggaagagcc ggggggattg cgcctcgggc aacgccttcc
     1381 catcgacggt gatcctgtcc ttggcgctca cgacatgagc cggcgtcgtc agcaccttgc
     1441 cgttcaccgc gacgcggccc gcctcgatca ttcgctcggc gtcgcggcgc gagcaaatcc
     1501 cggcatgggc gatcgctttg gcgatgcgca tcggctcagt gggctttgcc acctcgggct
     1561 tggatttcgg ccggttagcc ttgggccgac gcgccccttg acccgagctt cccttcccgc
     1621 ccactttgcc gcgcatgaat gagaagcttg cagatgaggc gcgaagaggc aaggccgcac
     1681 ggcccatgga cctcgcgctt gaggaggctg aagcggcggc cgcccgcggc gaggtgccga
     1741 tcggtgccgt cgtcgtcggt gcgtctggag aggttctggc caaggcctcc aaccgcacca
     1801 tcgagcttcg cgacccgacg gcgcacgccg agctccttgc cattcgcgcg gcttgttcga
     1861 agctgggcag cgagcggctt ctcgattgcg acctctatgt cacgctcgag ccttgcgcca
     1921 tgtgcgtagg cgccatctcc ttcgcgcgca tccgccgcct ctatttcggc gcggccgacc
     1981 cgaaaggagg cgcggtcgag cacggcccgc gcttcttcgc gcagccgacc tgccaccacg
     2041 cgccagaggt gatcggcggc ttgggcgaag cgcgggcttc agcgctgctt aagtccttct
     2101 tcgccgcgcg ccgctgaacg gtctagtgct cggtcagcat caagttccac tttgacggcg
     2161 tataccagag gcaggcgccg agcggtttgg accagaggca cacctggcca ggccgtggcc
     2221 ccgccagcgt gccgtgccgc ttggcggaga ggagcgcctg ctcccattgc tctcccgcgc
     2281 tttggtgcgc gagtgcccct tcggtcgaca aggcgataag acaaatagcc agaaatattg
     2341 gtcgcatgac agcctccctg tttaggctgg gatagcggct gccctgctaa taaagtgtgg
     2401 attatgctag tcttgatcac gacaagtact tcacagcact tctgaacaac tatggggtta
     2461 ggaacgaatt gaagaatgct ccaataaaaa tttagtaaaa tcgagcggtt ctttgccgaa
     2521 acggcgttag cggtcctcag cgcttagaac tggaaataga tgaagctcag gggttggcct
     2581 tcgccgactt cgagcacgca gaatacggcg atgacgatga agccgatcgc caccggccat
     2641 gtcggccgaa gccacatggt gagctcggca ctcggcacct tgatcaacgc cagccccccg
     2701 gcgatcgcga tcggcagcac cgtcatccag ggccaggcct cgcccagtcc tcctccaccg
     2761 gcaaggccgg tcaccatgcg gccggcggcg gagatgtccg acgcgcgaaa cagcacgaac
     2821 agcaggatca cgaagaccat ggtgatagcc catgccagga gcggaggcag gggaatgccg
     2881 taatgttgcc acaggcggca gatgagcaag cccatgccgt ggccaagccc ccacaggacg
     2941 aacgtccatc cggcgccgtg ccacaggccg cacaggccca tagtgaccat cgtcgcccag
     3001 atatagacca cgaccccctc gcggctgccg ccgagcggga tgtagatata gtcgcgcaac
     3061 cagcgcgaca gagttatgtg ccatcgccgc cagaaatcgc gcacgctcaa ggcacggtaa
     3121 ggcgtgttga aattcagggg cagcatcagt ccgagcgtga ggccgatgcc catcgccatg
     3181 tccgaataag cgacaaagtc gaacaggatc tgcagcgaga aggcaagcgc gcctgccgtc
     3241 gcgtcggaaa atgtcggcac ctg
//
LOCUS       DATNAE010000006         7465 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_604699, whole genome shotgun sequence.
ACCESSION   DATNAE010000006 DATNAE010000000
VERSION     DATNAE010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 7465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7465
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604699"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VN518_00105"
     CDS             <1..275
                     /locus_tag="VN518_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HXE03006.1"
                     /translation="EAEPGMTVMEAAVKNSIPGIAAECGGACACATCHVYVDEAWREA
                     TGSPEQMEEDMLDFAFDVRSESRLSCQIKVTDALDGLVVRIPEKQI"
     gene            282..1310
                     /locus_tag="VN518_00110"
     CDS             282..1310
                     /locus_tag="VN518_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011662914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXE03007.1"
                     /translation="MTESVIKTDAIIIGAGPVGLFAVFELGLVDIKAHVIDILDRAGG
                     QCTELYPEKPIYDIPGLTVVTGQELTDKLMDQIKPFGAEFHFNQRVETLERLDGGFRV
                     TTDADTVIEAKVVVIAAGGGSFTPKRPPLPRIEEYEGKSVFYSVRKMESLRGRDVLIV
                     GGGDSALDWTLNLQPVAKSITLLHRRAEFRAAPASVQKMMALVDSGDISFKLGQVTEL
                     HGENGQLNAVTVKGPDGATFEQPAEIMMPFFGLTMKLGPVADWGLELHENLVPVDTAI
                     FETSEKGIFAIGDINTYPGKLKLILSGFHEAALMAQQAHKYVYPDKKLIFQYTTSSSS
                     LQKKLGVK"
     gene            complement(1316..2284)
                     /locus_tag="VN518_00115"
     CDS             complement(1316..2284)
                     /locus_tag="VN518_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03008.1"
                     /translation="MLNPHRAVQETGNPGFYPPHPGIGAAPASKAETANAAELEAVRS
                     QVAERLAGLADKTADLTKQATSSAVAASKVGIAKAAKIGRETVIPKMRERTRPDRLRK
                     DYRDFLLWLHANVLDSAIERLFFVPTKGHVPLAGLNVRGNNRASGHDYKPTPNFVFKW
                     ALAAVAEEDISRMSFVDYGAGKGRVMLLASQYPFTQVGGIEFAEELHDNATMNIAQFP
                     RSQMKCRNVECVLDDVVNITPVDGAAVHYFFNPFGPEIFAEVLKGIVASYHARPRRLY
                     VILIDMEAAELMHKTGVFQEIKLPTAERMQAQTLSPYTIAVYRSLA"
     gene            2525..3106
                     /locus_tag="VN518_00120"
     CDS             2525..3106
                     /locus_tag="VN518_00120"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HXE03009.1"
                     /translation="MRASIHRQSMRHELIAMLPRLKRFADVLVGEKREGKALLRRALQ
                     CMLAEHHRYQRGTPLDRFAFTEIYRLWLGELRDHADPLRQAKSSEEDFDRIFPKDRGA
                     DFDTFTATFLGQLPPQQRSTLLLVYCEGFDYEGAAKVLDSDADTIQARLIRASASLAD
                     RLSPGSPAPAKASATIETLYPAEAQEYHHDRAQ"
     gene            3090..3497
                     /locus_tag="VN518_00125"
     CDS             3090..3497
                     /locus_tag="VN518_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03010.1"
                     /translation="MTELSDELLVAYVDGQLVREQTRAVDKVLEHDDVVARRVEALKN
                     AHGRFESAFEAILAGELIEILAAPILQPRPKRKRGNGLAKIGIAVVGIGIALAVLVGG
                     YGWPLVLRACSVTGPVATIQSAPAKSQSTPDAH"
     gene            complement(3494..3910)
                     /locus_tag="VN518_00130"
     CDS             complement(3494..3910)
                     /locus_tag="VN518_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03011.1"
                     /translation="MLLAAASIILAGCSTVERLGGTKKEGVSTDSAARYVTPEDPMAR
                     PIQVAWTSARASNCGFMFDPVKLKDDFMRNESRTVTDPYQLQRISQAYDYTLASVGDT
                     IKSDPKYCTRERTDAIRADLRRYLAGDYSPTAKLAR"
     gene            complement(4635..5612)
                     /locus_tag="VN518_00135"
     CDS             complement(4635..5612)
                     /locus_tag="VN518_00135"
                     /inference="COORDINATES: protein motif:HMM:TIGR01620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HXE03012.1"
                     /translation="MTKPRKPASFRLEEIEVVDTPLPPPVIATEAAQLPATVRGLPAL
                     ERGWRWGSILLAAVGGLVGLASVVWLHDLVREMMARQDWIGWTTIGLLGLAVLALLMI
                     ILREAAGLARLGRLGRLRHEADAAALEGDKALALAVVSRLKRLYHGRKDLGWGLARLA
                     EHEADILDAAELLRLSERTLVAPLDSIARSIVAASAKRVSVVTAVSPGALIDMLFVAS
                     ENLRMLRRLATLYGARPGTLSLLKLARMVVTHIVLTGGIALGDDVVQQLVGHGLTAKL
                     SARLGEGLFNGALTTRIGLAAIDVCRPLPYIEVQRPRFRELVAEVAGLR"
     gene            complement(5609..7057)
                     /locus_tag="VN518_00140"
     CDS             complement(5609..7057)
                     /locus_tag="VN518_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007066920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HXE03013.1"
                     /translation="MPISSFTHGAADAWRNIGDFASDLVEPTVRLGVTGLARSGKTVF
                     ITALVHNLMAGARMPFFDAVAHGRVLRAYLEPQPDDQVPRFDYERHMADLTASPPRWP
                     ESTRRISQLRLAVECEPQRFWKRRLGYNRLNIDIVDYPGEWLLDLPLLRLDYRQWSQQ
                     ALEQSRSASRRAIAKSALAEIDPAAPADEVVAQRLADLFKAYLRETRADNEALSTLPP
                     GRFLMPGDLEGSPALTFAPLDAQGRESFPRGSLFAMMERRYEAYKTHVVRPFFRDHFA
                     RLDRQIVLIDVLAALNGGKPAVADLERSMTKILECFRTGSNSLWSSLFAPRIDRIVVA
                     ATKADHLHHTSHDRLEAILALLTEDAMGRARYAGAEVKALALAAIRATKEGTAKRNGQ
                     ALPCIVGYPLPGETIGRRHFDGNEAFAIFPGDLPANPETALRHPHAQADDVRFVRFRP
                     PDPVPLPSGGFAPFPNIRLDRALQALIGDRLT"
BASE COUNT         1323 a         2361 c         2460 g         1321 t
ORIGIN      
        1 tcgaagccga gccggggatg acggtgatgg aggcggcggt caagaactcg atccccggca
       61 tcgccgccga atgcggcggc gcttgcgcct gcgccacttg ccacgtctat gtggacgagg
      121 cctggcgcga ggcgaccgga agtccggagc aaatggagga ggacatgctc gacttcgcct
      181 tcgatgtgcg gagcgaaagc cggttgagct gccagatcaa agtcaccgac gcgctcgacg
      241 gcctcgtggt gcgcatcccc gagaagcaga tctagttgcc catgaccgag tctgtcatca
      301 aaaccgatgc catcatcatt ggcgcaggac ccgtgggcct gttcgccgtg ttcgagctcg
      361 gcctcgtcga tatcaaagcg cacgtcatcg acattctcga ccgagctggc ggccaatgca
      421 ccgagctcta ccctgagaag ccgatctacg acatccccgg gctcaccgtc gttaccggtc
      481 aggagctgac cgacaagctc atggaccaga tcaagccctt cggcgccgag ttccatttca
      541 atcagagggt ggagacgttg gagcggctcg acggcggctt tcgcgtcacc accgatgccg
      601 acaccgttat cgaggccaag gtggtggtga tcgcggctgg tggcggctcg ttcacgccga
      661 agcgtccgcc gcttccgagg atcgaggaat acgaaggcaa gtcggtgttc tactcggtgc
      721 gcaagatgga gagcttgcgc ggccgcgacg tgctcatcgt cggcgggggc gattcagcgc
      781 tcgactggac cttgaacctg cagccggtgg caaagtcgat cacgctgctg catcgccgcg
      841 cagagttcag ggcggcgccg gcctcggtgc agaagatgat ggcgctcgtc gacagcggcg
      901 acatcagttt caagctcggt caggtgactg agctgcacgg cgagaatggt cagctcaacg
      961 cggtgacagt gaaaggcccc gacggcgcca ccttcgagca gccggccgag atcatgatgc
     1021 cgttcttcgg gctcaccatg aagctcggcc cggtggccga ttggggcctt gagctgcacg
     1081 aaaacctcgt cccggtcgac actgcgatat tcgagacgag cgagaagggc atcttcgcca
     1141 tcggcgacat caacacctat ccgggcaagc tcaagctcat cctgtcgggc ttccatgagg
     1201 cggcgctcat ggcgcagcag gctcacaaat acgtctatcc cgacaagaag ctgatcttcc
     1261 aatacacgac gtcgtcgtcg agcctgcaga agaagctcgg cgtcaagtag cgcactcagg
     1321 cgagcgatcg gtagacggcg atcgtgtacg ggctcagcgt ctgagcctgc atgcgctcgg
     1381 cggttggcaa cttgatctcc tggaacacgc cggtcttatg catgagctcg gcggcctcca
     1441 tgtcgatcag aatcacatag agacggcgcg gccgcgcgtg atacgaggcg acgatccctt
     1501 tcagcacctc ggcgaagatc tccggcccga acgggttgaa gaaatagtgc accgccgcgc
     1561 cgtctaccgg cgtgatgttg accacgtcgt cgagcacgca ctcgacgttg cggcatttca
     1621 tctggctccg cggaaattgc gcgatgttca tcgtggcgtt gtcatgcagt tcctcggcga
     1681 actcgatgcc gccgacttgg gtgaagggat attgcgaggc gagcagcatg acccgcccct
     1741 tgccagcgcc atagtcgacg aagctcatcc ggctgatgtc ttcctcggcg accgccgcaa
     1801 gcgcccattt gaagacgaaa tttggcgtgg gcttgtagtc gtggccgctc gcccggttgt
     1861 tgccgcgcac gttaaggccc gcgagcggca catgcccttt ggtgggaacg aagaacaggc
     1921 gctcgatcgc gctgtcgagc acattggcgt gcagccaaag caggaagtcg cgatagtctt
     1981 tcctgagcct gtccgggcgc gtgcgttcgc gcatcttggg gatgacggtt tcgcggccga
     2041 tcttcgccgc cttggcgatg ccgaccttgc ttgcggcgac cgccgacgag gtggcctgct
     2101 tggtaaggtc ggctgtcttg tcggcgaggc cggcgaggcg ttcggcgact tggctccgca
     2161 ccgcttcaag ctcggcggcg ttcgcggttt cggccttgct cgctggcgcc gcgcctatgc
     2221 ccggatgcgg cggatagaat cctgggttgc cggtctcctg aacggctcgg tgagggttga
     2281 gcattctctt cagatcccga ctccggttgg ttcgcgtcgt ggggcacgac cgacatgcgc
     2341 aagtcttgct cctgccttgc gcaaacaata ggcagcctca ccttcgacgc aagcgctttt
     2401 tgcccgggcg ttaacgctgc aggcgtcgaa tcggcgatgt ggtatcctag tgattcgcgt
     2461 ggagagaggc ccccttcgcg cgtcaagtgc cagcactcac gggaagcggc ggggggaacc
     2521 acgcgtgcgg gcaagcatcc atcgccaatc gatgcggcat gagctcatcg ccatgctgcc
     2581 gaggctcaag cgttttgccg acgtgctggt tggcgagaag agggagggca aggcgctcct
     2641 tcgccgcgcg cttcagtgca tgcttgccga gcatcaccgg tatcagcgcg gcaccccgct
     2701 cgaccgcttt gccttcaccg agatttatcg gctgtggctc ggcgagctgc gcgatcacgc
     2761 cgacccgttg cgccaggcca aatcgagcga ggaggatttc gaccggatct ttcccaagga
     2821 tcgaggcgcc gatttcgata ccttcaccgc gacgtttctc ggacagttgc cgccgcagca
     2881 gcgaagcacg ctgctcctcg tctattgcga gggctttgac tacgagggtg ccgccaaggt
     2941 cctcgattct gatgccgaca cgattcaggc gcgcctgatc cgggcgagtg caagccttgc
     3001 cgatcgcttg agcccgggct cgccggcgcc ggcgaaagcg agcgccacca tcgagactct
     3061 ctatccggca gaggcacagg aataccacca tgaccgagct cagtgacgaa ctgctcgtgg
     3121 cctatgtcga cggccagctc gtgcgcgagc agacgcgcgc cgtcgacaag gtgctcgagc
     3181 atgacgatgt ggttgcccgc cgcgtcgagg cgttgaagaa cgcgcatggc aggttcgaat
     3241 cagccttcga ggcgatcctc gccggcgagc tgatcgagat cctcgccgcg cccatcctgc
     3301 agccacggcc caagcgcaag cgcggcaacg ggctcgccaa gatcgggatt gcggtcgtcg
     3361 gcatcggcat cgcgctcgcc gtgctggtcg gcggctatgg ttggccgctt gtcctcagag
     3421 cctgcagtgt gaccggaccg gttgccacga tccagagcgc gccggcgaag tctcaatcga
     3481 cgccggacgc gcactagcgc gctagcttcg ccgtcggcga gtagtctccg gcaagatagc
     3541 gcctgagatc ggcgcggatc gcgtcggtgc gctctctggt gcagtatttg ggatcggact
     3601 tgatcgtgtc tcccaccgac gcgagcgtgt aatcgtaggc ttgggagatc ctctggagtt
     3661 gatagggatc ggtgaccgtt cggctctcgt ttcgcatgaa gtcgtccttg agcttcaccg
     3721 gatcgaacat gaatccgcaa ttgctcgccc tggccgaagt ccatgccacc tgaatgggcc
     3781 gcgccatggg gtcctcggga gtcacgtagc gtgctgcgct gtccgtgctg acgccttcct
     3841 tcttggtgcc accaagccgt tcgaccgtcg agcaaccggc gagaatgatg ctcgcggcag
     3901 ccaacagcac aagagggaac ccaaccccgc aacggcccaa cattctacgc ttcccaccca
     3961 ctgacgagat catggcggcg gcatcttagc ggaccgggac gcgcgcgcta agaggacgaa
     4021 atcgcttacg aaagggggag cacaaaaagg aaaggcggag ccgaagcccc gcctccctgt
     4081 gcgatgtcga gcctcacgcc cgttcgggac gatcatctcg gcgccgcgag acctttcggt
     4141 ctctggacca cgcgctcggc ttcatggttc cccacaaagc ggcggcgtga ccccggccta
     4201 ggcccgttat ttaacgtcgc gtggctgcga cgcgatccca atcttggcca gattggaacc
     4261 ggggttcggc ggttgagcgt gctgcgcaaa gagccccatc gactctacct cacggcattg
     4321 cggcaaagcc gacaggccgt ggcactctgc gcttgctaac ccctccgcct ttagccgccc
     4381 atggctgtgg acgacttagg gcttgcctag cccgagtgag ttttcggatc gtctccagcg
     4441 tcgtcggatt cctcctcaga caccttcgga ttgctcttca ggcagctttt ggatgactcc
     4501 ttcagcgcct tcgacaaccc gtccgctcac agttgttatc ctacatgagg ccgcgcgcgg
     4561 cgcgaggggg agcgaatcgc ttatccccat tatccactgg gcctgtgtcc agcgtgcaac
     4621 gctcgattcg cccgttagcg aagtccggca acttcagcca caagctcgcg aaagcgcggc
     4681 cgctgcacct cgatataggg cagcggacgg caaacatcga ttgccgcgag cccgatgcgg
     4741 gtggtgagcg cgccgttgaa cagcccttcg ccgagacgcg ccgagagctt cgccgtaagc
     4801 ccgtgcccga cgagctgttg gaccacgtcg tcgccgagcg cgatcccgcc cgtgagcacg
     4861 atatgggtga cgaccatgcg ggcgagcttg aggagcgaga gcgtgccggg gcgcgcgccg
     4921 tagagcgtgg cgagccggcg cagcatgcgc aaattttcgg aagccacgaa cagcatgtcg
     4981 atcagcgcgc cggggctcac cgcggtgacc accgaaaccc gcttggcgga ggccgcaacg
     5041 atgcttcggg cgatcgaatc gagcggggcg acgagcgtgc gctctgacaa ccgcaacagc
     5101 tcggccgcat ccaagatgtc ggcctcgtgc tcggcgagcc gcgccagacc ccaaccgagg
     5161 tccttgcgcc catgatagag gcgcttcagc cggctcacga cggcgagcgc gagcgccttg
     5221 tcgccttcga gcgcggcggc gtcggcctca tggcgaagcc gtccgagccg gccaagccgg
     5281 gcgaggccag cagcctcgcg caggatgatc atcagaagcg cgaggacggc gaggccgagg
     5341 agcccgatgg tggtccagcc gatccaatcc tgccgcgcca tcatctcgcg cacgaggtcg
     5401 tgcagccaga ccaccgaggc gagcccgacg agcccgccga cggcggcaag cagaatgctg
     5461 ccccaacgcc agccgcgctc gagcgccggc aagccgcgga cggtcgccgg cagttgtgcc
     5521 gcctcggtgg cgatcaccgg cggcggcagc ggcgtgtcga ccacttcgat ctcttcgagc
     5581 cggaaggagg ccggtttgcg tggcttggtc atgtgaggcg gtctccgatc agcgcctgga
     5641 gcgcgcggtc aagacggatg ttgggaaagg gcgcgaagcc gcctgaagga agcggcaccg
     5701 ggtcaggcgg ccggaagcgc acgaagcgca cgtcatctgc ttgcgcatgc ggatggcgga
     5761 gagcggtctc tggattggca ggcagatcgc cggggaagat ggcgaaggct tcattgccgt
     5821 cgaagtgccg ccgcccgatg gtttcccccg gcagcggata gccgacgatg caaggaagtg
     5881 cctggccgtt ccgcttcgcc gtgccttcct tcgtcgccct gatggcggca agtgcgagcg
     5941 ccttcacctc ggcgccagca tatcttgccc gccccatggc gtcctcggtg agcaaagcga
     6001 ggatcgcctc gagccggtcg tgactcgtgt gatgcagatg gtcggccttg gtcgcggcga
     6061 ccacgatgcg gtcgatgcgc ggagcgaaca ggctcgacca caggctgttc gatccggtcc
     6121 tgaagcattc gagaatcttg gtcatcgagc gttcgagatc ggcgaccgcg ggcttgccgc
     6181 cgttcagcgc ggcaagcacg tcgatcagca cgatctggcg gtcgagccgt gcgaaatgat
     6241 cgcggaagaa cggcctcacc acatgcgtct tgtaggcctc gtagcgccgc tccatcatgg
     6301 cgaagagcga gccgcgcgga aagctctcgc gcccctgcgc gtcgagcggg gcgaaggtga
     6361 gcgctggcga cccttcaaga tcgccaggca tgaggaagcg tcccggcggc agcgtcgaaa
     6421 gcgcctcatt gtcggcgcgc gtttcgcgca agtaggcctt gaacagatcg gcgagtctct
     6481 gcgccaccac ctcgtcggca ggggctgcgg gatcgatctc ggcgagcgcc gacttggcga
     6541 tcgctctccg gctcgccgag cggctctgct cgagcgcctg ctgcgaccat tgccggtagt
     6601 cgagccgcaa gagcggcaga tcgagcagcc actcgccggg gtagtcgacg atgtcgatgt
     6661 tgagccggtt ataaccgagc cgccgcttcc agaagcgctg cggctcgcac tcgacggcaa
     6721 gcctgagctg gctgatgcgg cgcgtgcttt ccggccagcg cggcgggctc gcggtgaggt
     6781 cggccatgtg ccgctcatag tcgaagcgcg gcacctgatc gtccggctgc ggctcgagat
     6841 aggcgcggag cacgcgcccg tgggcgacgg cgtcgaagaa cggcatgcgg gcgcccgcca
     6901 tgaggttgtg cacgagcgcg gtgatgaaca cggtcttgcc ggagcgggcg aggccggtga
     6961 cgccgagcct gacggtcggc tcgaccaggt ctgacgcgaa gtcgccgata ttgcgccaag
     7021 cgtcggcggc accgtgggtg aaggaagaga tcggcacgct tctcgatgtc ctttagcgac
     7081 tcttggcgcg ttgggcggga tgacgaaaag gtggggtgtg caacctcaag acccaacccg
     7141 ctgccccggt gattctcatc tcaatggcct gtcattcgcc tgtaatcgga ctctgactct
     7201 atcccgccgc agcgtagcgg ctggctcctg agcaggattt ttgtcaagcc cttctgctca
     7261 cttgacttgg tgccccgctt ccgtaaatac ggtgccgacc ggcgggggcc gcgattgctc
     7321 tcaaaccggc tggggacacg acggggcctt tggcccgagg aataaagacc atgcggttgg
     7381 cagaggcttt catgcggccg aagcgtaaaa tccccgaaga ctatcggccc tccgacgatg
     7441 agcctttcat gaacgagcgc cagcg
//
LOCUS       DATNAE010000007         3733 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_1810042, whole genome shotgun sequence.
ACCESSION   DATNAE010000007 DATNAE010000000
VERSION     DATNAE010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3733
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1810042"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1178
                     /gene="hemA"
                     /locus_tag="VN518_00145"
     CDS             <1..1178
                     /gene="hemA"
                     /locus_tag="VN518_00145"
                     /EC_number="2.3.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003870 - 5-aminolevulinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5-aminolevulinate synthase"
                     /protein_id="HXE03014.1"
                     /translation="YRVFAELKRRCGAYPAANQFTGQGTRDVTVWCSNDYLGMSQHKV
                     VLAAMHEAIDTVGAGSGGTRNISGTTHYHVELEAELADLHGKEAALLFTSAYIANDAT
                     LSTLQRLIPGLVIFSDEKNHASMIQGIRHGGGEKQIFRHNDVEHLEENLKKYPRGTPK
                     IIVFESVYSMDGHIAPIEAIADLAEKYNALTYLDEVHAVGLYGPRGGGIAERENVMHR
                     IDIINGTLAKGFGVMGGYIAATKACCDAIRSYAPGFIFTTSLAPAIVAGALASIRHLK
                     VSNVERMRMQERVLTLKTQLIEAGLPVMDNPSHIVPVMVGDPVHCKAVTDTLLDHYGI
                     YVQPINYPTVPRGTERMRLTPSPVHTDEQMAALIAVLQELWARCPVANGQYVRLAAE"
     gene            1230..1970
                     /locus_tag="VN518_00150"
     CDS             1230..1970
                     /locus_tag="VN518_00150"
                     /inference="COORDINATES: protein motif:HMM:NF042560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6675 family protein"
                     /protein_id="HXE03015.1"
                     /translation="MRTLLAIAVLLCSAGSCAAGQGPQPPCAGVEPVPAYAASEAPPN
                     SHLWTSAELGNWTPPACTGFTAKGDGVLVAVAGSFPFAGSAGALLARFGAVSALKGLK
                     YWSVTEVGWRTLITKATALEGPKVDRPRPDFTLAEMRSGADLYFAQSENRAGGDVVYR
                     MRVLYFGRDRFVVGIDNVSPVSRYLITLFNPGDLISVHFLERRGAGVWGYYGLAWAGD
                     SLVSRLAVPQASYVNRALALYRHFLGEE"
     gene            complement(2028..2423)
                     /locus_tag="VN518_00155"
     CDS             complement(2028..2423)
                     /locus_tag="VN518_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03016.1"
                     /translation="MSLLNLHLGDNADVNDGDFANTTYVGLSVALPLRMLNPSKRNQL
                     VIYTSAIETLADWQAHVVTLDFYFLGYLKDKPGSATERHFFDVPAFFIDGAPRRLNIA
                     VIGRGPGLEDDFVLKSIEASGFDLKLGWA"
     gene            complement(2469..2798)
                     /locus_tag="VN518_00160"
     CDS             complement(2469..2798)
                     /locus_tag="VN518_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXE03017.1"
                     /translation="MIKTGNLRSGLPAQPLPDEVVDLLVEHPGLRIERIVSTGQATPE
                     GQWYDQDRGELVLVVDGAARLRIEGEATDRELSEGDWILLPAHCRHRVTWTRAEPPTV
                     WLAIHFR"
     gene            2866..3486
                     /locus_tag="VN518_00165"
     CDS             2866..3486
                     /locus_tag="VN518_00165"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HXE03018.1"
                     /translation="MPGEPLTRERPIVKGRAALLIVDVQNGTFGPAQAKSKPEFHAHA
                     STRVIPNIRRLLEAFRRARLEVIYTVIENLTQDGRDRSLDYKLSGFNIPKGSWEARVI
                     DEIAPQEDEIVLPKTSSSPFNSTNLDYLLRNIGIEDVFVAGFLTDQCIDHVVKDGADR
                     GYYMTCVHDSCAAETEARHGAALQCFAGYCRLLPTDDVLRLTRDAQ"
     gene            complement(3480..>3733)
                     /locus_tag="VN518_00170"
     CDS             complement(3480..>3733)
                     /locus_tag="VN518_00170"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HXE03019.1"
                     /translation="GAMIFAGADGIARKLVLPKYDGEAPPLPRSLGEALDGMEKSAAI
                     KEWFGATFREAYLRHKRSELAHVAALDPPDLCARYAEVY"
BASE COUNT          697 a         1244 c         1122 g          670 t
ORIGIN      
        1 gctaccgggt ctttgccgag ctgaagcgcc gctgcggcgc ttaccctgcc gccaatcagt
       61 tcaccggcca aggcacccgc gacgtgacgg tatggtgctc caacgactat ctcggcatga
      121 gccagcacaa ggtggtgctc gccgccatgc atgaagcgat cgacacagtc ggcgccggct
      181 cgggcggcac ccgcaacatc tcaggcacca cccattatca cgtcgagctc gaggccgagc
      241 tcgccgatct gcacggcaag gaagcagcgc tcctctttac ctctgcctat atcgccaacg
      301 atgcgacgct ctccaccctg cagcgcctca ttccaggcct cgtgatcttc tccgacgaga
      361 agaaccacgc ctcgatgatc cagggcatcc gccatggcgg cggcgagaag caaatcttcc
      421 gccacaacga cgtcgagcat ctcgaagaga atctcaagaa atatccccgc ggcacgccga
      481 agatcatcgt gttcgagagc gtctattcga tggacggcca catcgccccg atcgaggcga
      541 tcgccgacct tgccgaaaag tacaacgcgc tcacttatct cgacgaagtg catgcggtcg
      601 gcctttacgg cccgcgcggc ggcggtatcg ctgagcgcga gaacgtgatg catcgcatcg
      661 acatcatcaa tggcacgctc gccaagggct tcggcgtgat gggcggttat atcgccgcga
      721 ccaaggcctg ctgcgacgcc atccgctcct atgccccagg cttcatcttc accacctcgc
      781 tcgcgcctgc catcgtcgcc ggcgcgctcg ccagcatccg tcatctgaag gtgagcaatg
      841 tcgagcgcat gcgcatgcag gagcgcgtgc tgacgctgaa gacccagctc atcgaggctg
      901 gcctgccggt gatggacaat ccgagccaca tcgttccggt gatggtcggc gatccggtgc
      961 actgcaaggc ggtgaccgac accctgctcg accattacgg aatctacgtg cagccgatca
     1021 actatcccac ggtgccgcgc ggcaccgagc gcatgcggct cacgccctcg ccggtgcaca
     1081 ccgacgagca gatggcggcg ctgatcgcgg ttctgcagga gctgtgggcg cgttgcccgg
     1141 tggccaacgg ccaatatgtg aggctcgccg cggagtagcc gcgttcgcgg attagcctgc
     1201 ttctccgatc ggaatgagcg cttctctcct tgcgcacgct gctcgctatc gccgtgctcc
     1261 tatgctcggc cggctcatgc gccgccgggc agggcccgca gccgccttgc gcaggtgttg
     1321 agccggttcc ggcctacgcg gcgtctgagg ctccacccaa cagccattta tggacgagcg
     1381 ctgagctcgg caactggaca ccgccagcct gcaccggctt caccgcaaag ggcgacggcg
     1441 tcctggtcgc cgttgccggc agctttccgt tcgccggaag cgccggtgcc ttgctcgcgc
     1501 gcttcggcgc cgtctcggcg ctcaaggggc tgaaatattg gtcggtgacc gaagtcggct
     1561 ggcgaacgct gatcaccaag gcgaccgcgc tcgaaggccc caaagtcgac cgcccgcgcc
     1621 cggacttcac cctcgccgag atgcgaagcg gcgccgatct ctacttcgcg caaagtgaaa
     1681 accgcgctgg cggcgacgtc gtctatcgga tgcgggtgct gtacttcggg cgagatcgct
     1741 tcgtggtcgg aatcgacaat gtgagcccgg tcagccgcta tctcatcacg ctgttcaatc
     1801 ccggcgatct catttccgtg cactttctcg agcgcagagg cgccggggtt tggggctatt
     1861 acggcctggc ctgggccgga gacagcctcg tctctaggct cgccgtgccg caggcctcct
     1921 acgtcaatcg cgctttggcc ctctaccggc atttcctcgg cgaagagtaa ctctaggcga
     1981 gtcgtacggc tagctgttga agaggcggcc tctcggcacg ggctcaatca cgcccagccg
     2041 agcttgagat cgaagccaga cgcctcgatc gacttcagaa cgaagtcgtc ctcgagccca
     2101 ggcccgcgtc cgatgaccgc tatgttgagg cgcctcggcg ctccatcaat gaagaaggcg
     2161 ggaacgtcga agaagtgccg ctctgtggcg gagccaggct tgtccttgag atatccgaga
     2221 aagtagaaat cgagcgttac gacatgggct tgccagtcgg ccagggtctc gatcgccgac
     2281 gtatagatga caagctggtt cctcttgctg ggattcaaca tgcgcagagg caaggcaacg
     2341 ctcaagccaa cataggttgt gttggcgaaa tcgccatcgt tgacgtctgc attgtctccc
     2401 aagtgcaaat tgagcagtga catgggttcg cctccggcag ggtgcgtctt acagggttta
     2461 cctctgcgtt agcgaaaatg tatagcgagc cagacggtcg gcggctctgc cctcgtccag
     2521 gtcacgcgat gccggcaatg ggccggaagt aggatccagt cgccttcgct gagctcgcgg
     2581 tcggtcgcct cgccctcgat gcgaagccgc gccgcgccat ccaccacaag cacgagctcg
     2641 cctctgtcct ggtcgtacca ttggccttca ggcgtcgcct gaccggtcga cacgatgcgc
     2701 tcgatgcgga ggcccgggtg ctcgacaagc agatcgacga cctcatctgg caggggctga
     2761 gccggtaggc cgcttcgaag attgccggtc ttgatcacgg ccaaagccta gcacaggccc
     2821 ccgccaggac ttgctgccaa cgccgagatg ccggaaaagc aggtcatgcc aggagagccc
     2881 ctcacccgcg agcggccgat cgtcaaaggc cgcgccgccc ttctcatcgt cgacgtgcag
     2941 aacggcacct tcggtccggc gcaagcgaag tccaagcccg aattccacgc gcacgcgtcg
     3001 acgcgcgtga tccccaacat aaggcgtttg ctggaggcgt tccggcgcgc gcgcctcgaa
     3061 gtgatctaca ccgtgatcga gaatttgacc caagacggac gcgaccgcag cctcgactac
     3121 aagctttcgg gcttcaacat ccccaagggc tcatgggagg cccgcgtgat cgacgagatc
     3181 gcgccgcaag aagatgagat cgtgctgccc aagacctcct cctctccgtt taactccacc
     3241 aatctcgatt atctgctccg caatatcggc atcgaggatg tgttcgtcgc cggcttcttg
     3301 accgaccagt gcatcgacca cgtggtcaag gacggcgccg accgcggcta ctacatgacg
     3361 tgcgtgcacg attcctgcgc cgccgaaact gaagcgcgcc acggcgcggc gctgcaatgc
     3421 ttcgcaggct attgccggct gctccccaca gacgatgttt tgcggctcac gcgcgacgct
     3481 caatagacct cggcgtaacg ggcgcagagg tccggcggat cgagcgcagc gacatgggca
     3541 agctcggagc gcttgtggcg aagataggcc tcgcgaaaag tcgcgccgaa ccattccttg
     3601 atcgcggcgc ttttctccat gccgtcgagc gcctccccaa gcgatcgcgg caaaggcggc
     3661 gcctcgccat catatttcgg caggacaagc ttccgcgcga tgccgtcggc cccagcaaag
     3721 atcatcgcgc caa
//
LOCUS       DATNAE010000008         4313 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2414437, whole genome shotgun sequence.
ACCESSION   DATNAE010000008 DATNAE010000000
VERSION     DATNAE010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4313
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2414437"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..2318)
                     /gene="ptsP"
                     /locus_tag="VN518_00175"
     CDS             complement(57..2318)
                     /gene="ptsP"
                     /locus_tag="VN518_00175"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019966074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HXE03020.1"
                     /translation="MPISVGAPRLLLRRLREVMAERESAQARLDKVVVLIASNMVAEV
                     CSLYLRRRDSSLELFATEGLNPEAVHNTHLKAGEGLVGLIAEHGEPMQFADAQVHPAF
                     SYRPETGEEVYHSFLGVPILRGGHTIGVLTVQNRTQRHYSDEEVEALQTTAMVIAEMI
                     ASGGLVVSEEGGQEARREQSARFTGVTISEGVALGHVVLHEPRIVVTKLIAEDVEFER
                     RRLAQAIDELTGMIDGMLERGDLQRAGEHREVLEAFRMFAHDRGWRRRLDEALLTGLT
                     AEAAVQRVRNDTRARMLRQIDPYARERLHDIDDLSNRLLRVLAGGAGTAAAGNLPQDT
                     ILIARTMGPAELLDYDRQRLRGLVLEEGGSASHVAIVARALGIAAISQAKGVLDAVEP
                     GDAAIVDAEGGELHVRPTPEVVSAYADKVRFRARRQAQYAALRNVPAVTLDGARVSLN
                     INAGLVFDLPHLDQSGADGIGLFRTELQFMIASTFPRLEQQTRIYKAILDAAGERPVT
                     FRSLDVGGDKVLPYFRVSKEENPALGWRAIRMALDRPALFRTQVRALLRAAHGRELRI
                     MLPMIGDVAELTAARALIDREVTILKRHARPEPKKLLVGAMIEVPALLYQLERLLPLA
                     DFASVGSNDLMQFLFATDRGNMLVADRFDNLNPAALLALRVIVEAAGRHNTPLTLCGE
                     MAGKPIEAMALIGLGFRSISMAPASVGPVKAMVLSVDAGELWARLQELLATKSTSLRE
                     ELKSFAAEKGVQI"
     gene            complement(2393..3670)
                     /locus_tag="VN518_00180"
     CDS             complement(2393..3670)
                     /locus_tag="VN518_00180"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HXE03021.1"
                     /translation="MPLLVLKFGGTSVADVERIRNVAAHVKREVDAGHACAVVVSAMA
                     GQTNQLVSWVEDAWGFKQKNGNQVSAMYDAREYDAVVASGEQVTSGLLAIVLQSIGVK
                     ARSWQGWQIPMTTDGAHRAARITGVAVETLKARMLNGEVAVVAGFQGIGPENRISTLG
                     RGGSDTSAVALAAALDADRCDIYTDVDGVYTTDPRIVPKAHRLDRISYEEMLEMASQG
                     AKVLQTRSVELAMQRGVRVLVRSSFEPPNGPRADTEACGTLVCHEDEIVEQNVVSGIA
                     YARDEAKVTLLKIADKPGVAAQVFCPLAEAHINVDMIVQNVSEDGFTDLTFTVQSADF
                     DRAIEVLRKSRDAIGYADLRGSSDIAKVSAIGVGMRSHAGVASQMFSALAEKGINIEA
                     ISTSEIKISVLIDAAYAELAVRTLHSLFGLDSR"
     gene            3850..>4313
                     /gene="ubiG"
                     /locus_tag="VN518_00185"
     CDS             3850..>4313
                     /gene="ubiG"
                     /locus_tag="VN518_00185"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HXE03022.1"
                     /translation="MAHAPHAPLDPPTLDREEVARFARLSGQWWDELGPFRQLHRINP
                     VRLTYIRDQLCRRFGRDAKGPASLSGLSVLDIGCGGGLVCELLARLGAGVTGIDPAVE
                     NIEAAKAHASAGGLDIAYEGTTAEELASRGKSYDAVLLLEVVEHVPDLQAFI"
BASE COUNT          716 a         1470 c         1413 g          714 t
ORIGIN      
        1 ccacgctttg caagcctccg cgctgccgca aaacgccgga tcctggccga ctgtcgtcaa
       61 atctgcacac ctttttcggc tgcgaagctc ttgagctcct cacgcaagga agttgatttg
      121 gtggcaagca gttcctgcaa ccgcgcccac aattcgccgg catcgacgga gagcaccatg
      181 gccttgacgg gcccaaccga ggctggcgcc atggagatcg agcggaagcc gaggccgatc
      241 agggccatcg cctcgatcgg cttgccggcc atctcgccgc agagcgtgag cggcgtgtta
      301 tgtctccccg ctgcttccac gatcacgcgg agcgccagca gcgccgccgg gttcaaattg
      361 tcgaagcggt cggcgacgag catgttgccg cggtcggtgg cgaacaggaa ctgcatcaga
      421 tcgttcgagc cgaccgaggc gaaatcggca agcggcagca ggcgctcgag ctgatagaga
      481 agcgccggga cctcgatcat cgcgccgacc agcagcttct tcggctcagg cctggcgtgg
      541 cgcttgagga tggtgacctc gcggtcgatc agcgcgcgcg ccgccgtaag ctcggccaca
      601 tcgccgatca tcggcagcat gatcctgagc tcgcgcccat gcgccgccct gagcagcgcc
      661 cgaacttgcg tgcggaacag agcaggccgg tcgagcgcca tgcggatggc gcgccagccg
      721 agcgccgggt tctcctcctt gctcacccga aagtagggca gcaccttgtc gccgccgacg
      781 tcgagcgagc ggaacgtgac cgggcgctcg cccgcggcgt cgaggatcgc cttgtagatg
      841 cgggtctgct gctcaagccg cgggaaggtc gaggcgatca tgaattgcag ctcggtgcgg
      901 aacaggccga tgccgtcggc gcccgactga tcgagatgcg gcaggtcgaa cacgaggccg
      961 gcattgatgt tgagcgacac ccgcgcgccg tcgagcgtga ccgccggcac gttgcgcaag
     1021 gccgcgtact gtgcttggcg cctcgcccga aagcggacct tgtcggcata ggccgacacc
     1081 acctcaggcg tcggccgcac gtgcaactcg ccgccctcgg catcgacgat cgccgcatcc
     1141 cccggctcga ccgcatcgag cacgcccttg gcctggctga tggcggcgat accgagcgcg
     1201 cgggccacga tggccacgtg gctcgccgaa ccgccttcct cgagcacgag cccgcgcaag
     1261 cgctgccggt cgtaatcgag cagctcggcg ggacccatgg tgcgggcgat caggatggtg
     1321 tcttgcggca gattgccggc ggccgccgtg ccggccccgc ccgcgagcac gcgtaagagc
     1381 cggttggaga ggtcgtcgat gtcgtgcaga cgctcccgcg cgtaagggtc gatctgcctc
     1441 agcattcggg cgcgggtgtc gttgcgcacg cgctgcaccg cggcttccgc ggtgaggccg
     1501 gtgagcagag cctcatcgag gcggcggcgc cagccgcggt cgtgcgcgaa catgcggaag
     1561 gcctcgagca cctcgcgatg ctcgccggcg cgctgcagat cgccgcgctc gagcatgccg
     1621 tcgatcatgc cggtgagctc atcgatggct tgtgccagcc gccggcgctc aaactcgacg
     1681 tcctcggcga tcagcttggt gaccacgatg cgcggctcgt gcagcaccac atggccgagc
     1741 gccacgcctt cggagatggt gacgccggtg aatcgggcgc tctgctcgcg gcgtgcctcc
     1801 tgcccgccct cctcgctcac cacgaggccg cctgaggcga tcatctcggc gatcaccatg
     1861 gcggtggtct gcaacgcctc cacctcctcg tcgctgtagt ggcgctgggt gcggttctgc
     1921 accgtcagca ccccaatggt gtggccgccg cgtaagatcg gcacgccgag gaaggagtgg
     1981 tagacctcct ccccggtctc aggccggtag gagaaggcgg ggtggacctg agcgtcggcg
     2041 aattgcatcg gctcgccgtg ctcggcgatc aagccgacca ggccctcgcc ggctttcaga
     2101 tgcgtgttgt gcaccgcctc ggggttcaag ccttcggtgg cgaacagctc gagggaagag
     2161 tcccggcggc ggagataaag cgagcacacc tcggccacca tgttggaggc gatcagcacc
     2221 acgaccttat cgagacgcgc ctgcgcgctc tcgcgctcgg ccatgacctc gcgcaagcgg
     2281 cggagcagca atctcggggc gccaactgaa attggcataa atcgagtcct cgccggcggc
     2341 ttaagcggcg aacctgagct ggcgaaaggc ttgaggtggc cgcgctgtgt gtctagcggc
     2401 tgtccagtcc gaacagcgaa tgcaaggtcc gcaccgcaag ctcggcgtag gccgcgtcaa
     2461 tcaatacgct gattttgatc tccgaggtgg aaatcgcctc gatattgatg cctttttcgg
     2521 ccaaagcgct gaacatttgc gaggcaacgc cggcgtggct gcgcatgccg accccgatgg
     2581 ccgacacctt ggctatgtcg gaggagccgc gcaaatcggc atagccgatg gcgtcccgcg
     2641 acttcctcag cacctcgatg gcgcggtcga aatcggcgct ctgcacggtg aaggtaaggt
     2701 cggtgaagcc gtcctccgag acgttctgca cgatcatgtc gacattgata tgggcctcgg
     2761 cgagcgggca gaacacctgg gccgccacac ccggcttgtc ggcgatcttg agcagggtca
     2821 ccttggcctc gtcgcgggca taggcgattc cgctgacgac attttgctcc acgatctcat
     2881 cctcatgaca aacgagtgtt ccacaggcct cggtatcggc gcgcgggccg ttcggcggct
     2941 cgaagctcga gcgcaccagc accctgacac cacgctgcat ggcgagctcc accgagcggg
     3001 tttgcagcac cttggcgccc tgcgaggcca tctccaacat ctcttcatag gagattctgt
     3061 caagccgatg ggctttcggc acgattctgg ggtctgtggt gtagacgccg tcgacgtcgg
     3121 tatagatgtc gcagcggtcg gcatcgagcg ccgcagccaa ggccacggcg ctggtatccg
     3181 agcctccgcg gccgagcgtt gagatgcggt tctcggggcc gatgccctgg aagcctgcca
     3241 ccaccgccac ctcgccgttc agcatgcggg ctttcagcgt ttccacggcg accccggtga
     3301 tgcgggcggc gcggtgggcg ccatcggtgg tcatcgggat ctgccagccc tgccaggacc
     3361 gcgccttgac cccgatcgac tggagcacga tggcgagcag ccccgacgtc acctgctcgc
     3421 ccgaggccac caccgcgtca tattcgcggg catcgtacat ggccgagacc tggttgccgt
     3481 tcttctgctt gaagccccag gcatcctcca cccaggagac gagctgattg gtctggccgg
     3541 ccatggccga aaccaccacg gcgcaagcgt ggcctgcgtc gacctcgcgc ttcacgtgcg
     3601 ccgcgacgtt gcggatgcgt tcgacgtcgg caaccgacgt cccgccaaat ttgagcacca
     3661 acaggggcac ttgcccctcc caggcctcgg gccgccatcg aggcgccact ggcccatagc
     3721 aacagcatgg ccggccccag ggccggccag aaagcggttc tacatactga ctggcggccc
     3781 tggccgcaac ctcaccccac cctctctcgc cattcttgac agcgggttaa gggccacggt
     3841 caatgcctca tggcccacgc cccccacgcc ccgctcgatc cgcccacgct cgaccgcgag
     3901 gaggtcgccc gcttcgcccg gctcagcggg caatggtggg acgagctcgg gcccttccgc
     3961 cagcttcacc gcatcaaccc ggtgcggctc acctatatcc gcgaccagct gtgccggagg
     4021 tttggacgcg acgccaaggg gcctgcaagc ctctcaggcc taagcgtgct cgacatcggt
     4081 tgcggcggcg gcctcgtctg cgagcttttg gcgcggctcg gggccggcgt caccggcatc
     4141 gaccctgcgg tggagaacat cgaggctgcc aaggctcatg cctcggccgg aggcctcgac
     4201 attgcctatg aggggacgac cgccgaggag cttgcctcgc gcgggaaaag ctacgacgcc
     4261 gtgttgctgc tcgaagtggt cgagcacgtg cccgacctgc aggcctttat caa
//
LOCUS       DATNAE010000009         2714 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_1813087, whole genome shotgun sequence.
ACCESSION   DATNAE010000009 DATNAE010000000
VERSION     DATNAE010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1813087"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="VN518_00190"
     CDS             <1..485
                     /locus_tag="VN518_00190"
                     /inference="COORDINATES: protein motif:HMM:NF013236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HXE03023.1"
                     /translation="LDIRFPIPPAITGDELQKQLLRDGPAAKLVFELSTSKSKGTLEL
                     FMPHRVLLSHRAERPGAADRAVAAKWGAHFGEEIMRSVVRLDAAIPMAPMTLGELAAL
                     QVGQVIEMPKTAQAETRLSAREKTLFVCEFGKIGANYTVRVRHPFDAGQQFIDGLFPG
                     "
     gene            516..905
                     /gene="fliN"
                     /locus_tag="VN518_00195"
     CDS             516..905
                     /gene="fliN"
                     /locus_tag="VN518_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008835487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HXE03024.1"
                     /translation="MSEPESKAPEADIEEAQLNQAIEDLRGALHDEPASGQGDQAASL
                     RSNAIIMNIPVDVQIMLGSTEMPVSELMALQKGSTVALDRRIGEPVDVVVNGQKIARG
                     EITVLDNDPSRFGVKLTEIITAGKAPR"
     gene            934..1956
                     /locus_tag="VN518_00200"
     CDS             934..1956
                     /locus_tag="VN518_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HXE03025.1"
                     /translation="MTQPLNLTKAQKAAAILVAMGKPAAGRLLKFFKQDELKALIDGA
                     RMLRTIPQGDLERIVGEFEAEFSEGAGLLDSADTMDTILNESFSPEEMKAILGQDQPK
                     AIESKAPPPVWPSVEKLEAERVGTFLANEHPQTIALVLTNMSPQPAANALMTLSKPVR
                     GEVVKRMLSMNNVPDQAKRIVEDQIRMRMLAETSFKDNTAGQIRVAGVLNELDKDALD
                     EVIGEMRAAGTSDLDAIQARLFNFDDIVQLSQKSRVALFDGLATDLVTLALRGCEPTL
                     AEAILSALGPRSRRMIESELAAEGGNIPFAEIARARKTVASTAIRLAGEGAVQLPGSE
                     EVQEAA"
     gene            1977..>2714
                     /locus_tag="VN518_00205"
     CDS             1977..>2714
                     /locus_tag="VN518_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007008642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HXE03026.1"
                     /translation="MAEDNDQESKTEEPSERKIRDTIEKGNLPFSKELSILASFLAIL
                     VFTVFLAQGSLVDLGSFLSGFLDRPEEWPLATAQDVATLYRMVLIEIAKSVGAVLILL
                     MAAGLAASALQNMPRLVLDRIQPKASRISLREGWKRLFGVQGFVEFGKSLGKLGFTAM
                     FVMLALNGIVQQLLSGMITQPTEFAMVIKEAATHILVAITLVMTAIAGADLFWSRFKW
                     RRDLRMTRQEVKDEFKQTDGDPHIKAKL"
BASE COUNT          519 a          891 c          871 g          433 t
ORIGIN      
        1 ccctcgatat ccgcttcccc atcccgcccg ccatcaccgg cgacgagttg caaaagcagc
       61 tccttcgcga cggtccggcg gccaagctcg tattcgaact ctcgacgtcg aagagcaaag
      121 ggacgctcga gctgttcatg ccgcaccgcg tgctcctctc gcatcgcgcc gaacgtcccg
      181 gcgccgccga cagggcggtc gcggccaaat ggggcgcgca tttcggcgag gagatcatgc
      241 gctcggtcgt caggctcgac gccgcaatcc cgatggcgcc gatgacgctg ggcgagcttg
      301 ccgcgcttca ggtcggccag gtcatcgaga tgcctaagac ggcgcaagcc gagacccggc
      361 tatcggccag ggagaagacg cttttcgtgt gcgaattcgg caagattggc gcgaactata
      421 cggtgcgcgt ccgccacccc ttcgacgccg gacagcaatt tatcgacggg ctctttcccg
      481 gctgaagccg tggcaaggcc gaatggagga tcgagatgag cgaacccgaa tcaaaggctc
      541 ccgaggcaga catcgaggaa gcacagctca accaggcgat cgaggatctg cgtggcgccc
      601 tgcatgacga gccggcctcg ggccagggcg accaggcggc cagcctgcgc agcaacgcga
      661 tcatcatgaa catccccgtc gacgtgcaga tcatgctcgg gagcaccgaa atgccggtct
      721 cggagctgat ggcgctgcag aaaggctcca cggttgcgct cgacaggcgg atcggcgagc
      781 cggtcgatgt cgtcgtcaac ggccagaaga tcgcccgcgg cgagatcacc gtgctcgaca
      841 acgatccatc gcgcttcggc gtcaagctca ccgagatcat caccgccggc aaggcgccgc
      901 gctgattttc cggatttcca gggatacagg ccgatgaccc agcccctcaa cctcaccaag
      961 gcgcagaagg ccgccgccat cctggtggcg atgggcaagc ccgcggccgg gcggttgctc
     1021 aaattcttca agcaggacga attgaaggcg ctgatcgacg gagcacgcat gctgcgcacg
     1081 atcccgcagg gcgatctgga gcggatcgtg ggcgagttcg aggcggagtt ctccgaaggt
     1141 gcgggcctgc tcgattcggc cgacaccatg gatacgatcc tcaacgagtc cttctcgcca
     1201 gaggagatga aggccatcct cggccaggac cagccgaagg cgatcgaaag caaggcgccg
     1261 ccaccggtct ggccttccgt cgagaagctc gaagccgaac gggtcggcac cttcctggcc
     1321 aacgaacatc cgcagacgat cgcgctcgtg ctcaccaaca tgtcgccgca gccggccgcc
     1381 aacgcgctga tgacgctctc caagccggtg cgcggggagg tggtgaagcg catgctctcc
     1441 atgaacaacg tgccggatca ggccaagcgg atcgtcgagg accagatccg gatgcgcatg
     1501 ctcgccgaga cctcgttcaa ggacaacacg gccggccaga tacgcgtggc cggcgtgctc
     1561 aacgagctcg acaaggatgc tctcgatgag gttatcgggg agatgcgggc cgccggcacg
     1621 tccgatctcg acgcgatcca ggcgcggctc ttcaatttcg acgatatcgt ccagctttcc
     1681 cagaagtcgc gggtggcgct cttcgacggg ctggcgaccg acctcgtcac gctggcgctg
     1741 cgcggttgcg agccgacgct tgccgaggcg atcctttcgg cgctcggccc gcgttcgcgc
     1801 cgcatgatcg aatccgaact cgccgccgag ggcggaaaca tcccgttcgc cgagatcgcc
     1861 cgggcgcgca agaccgtcgc ctcgaccgcg atccgcctcg ccggcgaggg ggcggtccag
     1921 cttcccggct ccgaagaggt gcaggaggcg gcctgagcga atcgagggcg tagttcatgg
     1981 ccgaggacaa cgatcaggaa agcaaaaccg aggaaccctc ggagcgaaag atccgcgaca
     2041 cgatcgagaa gggcaatctg ccgttctcga aggaactgtc catcctcgct tcgttcctgg
     2101 cgatcctggt gttcacggtc ttcctcgcgc agggcagcct cgtcgatttg ggaagcttcc
     2161 tgtccggctt cctcgaccgt cccgaggaat ggccgctcgc cacggcccag gacgtggcaa
     2221 cgctctaccg catggtgctc atcgagatcg ccaagagcgt tggcgccgtt ctcatcctgc
     2281 tcatggcggc ggggctggcg gcctcggcgc tgcagaacat gccgcgcctc gtgctcgacc
     2341 gcatccagcc caaggcgtca cgcatctcgc tgcgcgaagg ctggaaaaga cttttcggcg
     2401 tgcagggctt cgtcgagttc ggcaagtcgc tcggcaagct cggcttcacg gcgatgttcg
     2461 tgatgctggc gctgaacggc atcgtccagc aactgctctc cgggatgatc acgcaaccga
     2521 cggaattcgc gatggtcatc aaggaggcgg ccacgcatat cctcgtcgcc atcacgctgg
     2581 tgatgacggc gatcgccggc gccgacctgt tctggtcgcg cttcaaatgg cggcgcgacc
     2641 tcaggatgac gcggcaggag gtcaaggacg aattcaagca gaccgacggc gacccgcaca
     2701 tcaaggccaa gctc
//
LOCUS       DATNAE010000010         2510 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_910687, whole genome shotgun sequence.
ACCESSION   DATNAE010000010 DATNAE010000000
VERSION     DATNAE010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_910687"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2301
                     /locus_tag="VN518_00210"
     CDS             <1..2301
                     /locus_tag="VN518_00210"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HXE03027.1"
                     /translation="ILVDEAQDTNPEQWSIITRLAEEFFAGEGASDTLRTLFAVGDEK
                     QSIYSFQGADPVRFGTVGRAFRAKAMAIEQTWNDVPLTLSFRSTEAVLKAVDAVFNKR
                     PAADGVIWNDGDIIEHHAFRTGEAGLVELWPVVAETKIEPAEAFEPWNEDAVPPHAVD
                     VLCQRIAGQIKAWLDKDEELESEGRKIKAGDILILVRRREPFTAPMIRALKREKIAVA
                     GADRMQLLQQIAVMDLMALADVLLMPDDDLSLAVVLKSPLFGLDDDDLFELAFERRTS
                     LWNVLQAKAKENARCTEAAETLSRWLSRVDLAPPYEFFLELIGENCQAMRKRILTRLG
                     PETAESLDEFLDLALGFDRESPPSLQGFVNAMRSTDVEIKRDMEQKRDEVRIMTVHGA
                     KGLQAPIVFLPDTCMRPRPQGASIHLLARHGVPPEEVGHIVWPAGGNTLEHIEVAKDL
                     ARKAEIEEYHRLLYVAMTRARDRLYVCGWSQKEAPEKASWYELVYQGLQGLLTEGAGY
                     DGKPVQRLISKQDSPAKAPCEAEAATEAAELPDWASRPAPAERSRAILTPSGLGALLG
                     DSVSPYPEQPPLGPKALADSRRFARGRLVHTLLQHLPEVAAADREGAARNFVAARGRD
                     LPEEMREEIVSETLAIVQDTRFAPLFAPGSLSEVPIVARLGEGDISGQIDRLAVLDDA
                     LLVLDYKTNRPPPSVPDEVAPGYIAQLAAYRSALRLMFPERALRAAIIWTDGPKLMEI
                     PSTLLDLAERRMLQKGPSLDVAGART"
     gene            2372..2494
                     /locus_tag="VN518_00215"
                     /pseudo
     CDS             2372..2494
                     /locus_tag="VN518_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012456650.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
BASE COUNT          450 a          807 c          834 g          419 t
ORIGIN      
        1 atcctcgtcg acgaggcgca ggacaccaac cccgagcaat ggtcgatcat cacgcgtctt
       61 gccgaggagt tcttcgctgg cgagggggca agcgacacgc tgcgcaccct gttcgcggtt
      121 ggcgacgaga agcagtccat ctacagcttc cagggcgccg accccgttcg cttcggcacg
      181 gtcgggcgcg cgttccgcgc caaggcgatg gcgatcgagc agacctggaa cgacgtgccg
      241 ctcacccttt ccttccgctc gaccgaggcc gtgctcaagg cggtcgatgc ggtgttcaac
      301 aagcgcccgg ccgccgacgg cgtgatctgg aacgacggcg acatcattga gcaccacgcc
      361 ttccgcaccg gagaggccgg actggtcgag ctttggccgg tggtggccga gacgaagatc
      421 gagccggccg aagccttcga gccttggaac gaggacgccg tgccgccgca tgcggtcgat
      481 gtgctgtgcc aacgcatcgc cgggcagatc aaggcttggc tcgacaagga cgaagagctt
      541 gaatccgaag gccgcaagat caaggcaggc gacattctga tcctggtgcg gcgccgcgag
      601 ccgttcaccg cgccgatgat ccgcgccttg aagcgtgaga agatcgccgt ggccggcgcc
      661 gaccggatgc agcttctgca acagatcgcg gtgatggatc tcatggcgct cgccgatgtc
      721 ctgttgatgc cggacgacga tctttcgcta gccgtggtgt tgaagagccc gctcttcggg
      781 ctcgacgacg acgatctgtt cgagctcgct tttgagcggc gcacctccct atggaatgtt
      841 ctgcaggcca aggccaagga gaatgcgcgg tgcacggagg ccgccgagac tctgtcgcgc
      901 tggctctcgc gtgtcgatct cgccccgcct tacgaattct tcctcgagct gatcggcgag
      961 aactgtcagg cgatgaggaa gcgcatactc accagacttg gacctgaaac agccgagtct
     1021 ctcgatgagt ttctcgacct tgcgcttggc ttcgacaggg agtcgccgcc atcgctgcaa
     1081 ggcttcgtca acgccatgcg ctcgaccgac gtcgagatca agcgcgacat ggagcagaag
     1141 cgcgacgagg ttcgcatcat gacggtgcac ggcgccaagg gcctgcaggc gccgatcgtg
     1201 ttcctgcccg acacctgcat gcggccgagg ccccagggcg cgagcatcca tttgctcgca
     1261 cggcatggcg ttccgccgga ggaagtcggc catatcgttt ggcccgcggg cggcaatacg
     1321 cttgagcaca tcgaggtggc caaggacctc gcgcgcaagg ccgagatcga ggagtatcac
     1381 cggctgctct acgtcgccat gacgagagcg cgcgaccggc tctatgtgtg cggctggagc
     1441 cagaaggaag cgcccgagaa ggcgtcctgg tacgagctcg tctatcaggg gctgcaaggc
     1501 ctgctcaccg agggcgcagg ctatgacggg aagccggtgc agaggctcat cagcaagcaa
     1561 gactctccgg cgaaggcgcc gtgcgaggcc gaggcggcga cagaggcggc ggagctgccg
     1621 gactgggcaa gcaggccagc gccggcggag cgctcgcgcg cgatcctcac accctcaggg
     1681 cttggcgcac tcctcggcga tagcgtgtcg ccttaccccg agcagcctcc gctcggaccg
     1741 aaggcgctcg ccgacagccg gcgcttcgcc cgtggacggc tggtgcacac gctgctgcag
     1801 catctgccgg aggtcgcagc cgccgatcgc gagggtgcgg cgcgaaattt cgtcgccgcg
     1861 cgcgggcgcg acctgcccga ggagatgcgt gaggagatcg tgagcgagac gctcgccatc
     1921 gtgcaggaca cgcgcttcgc gccgctcttt gcgcccggca gcctcagcga ggtgcccatc
     1981 gtggcaaggc ttggcgaggg cgacatctcg ggccagatcg accgcctcgc agtcttggac
     2041 gacgctctcc tcgtgctcga ctacaaaacg aatcggccgc cgccttccgt ccccgatgag
     2101 gtggcgccgg gctacatcgc gcagcttgcc gcctatcgct cagcgcttcg cctgatgttt
     2161 ccggagcgag ctttgcgggc ggcgatcatc tggaccgacg gcccgaaact catggaaatc
     2221 ccatcaacac tgcttgattt ggcggaacgc cgcatgttgc agaaaggccc cagccttgac
     2281 gttgccgggg cgcgcaccta ggttcggttt cagaccacaa gaccggccac tttcgggggt
     2341 cgaacgccgg cgacctagga gaagcaaata gatggcaact gcaactgttt ccgacgatac
     2401 cttcgaggcc gacgtattga agtcgtccac ccccgtcgtg gtggacttct gggctgaatg
     2461 gtgcggtcct tgccggcaga tcgcaccggc gctctaggag atcgccaagg
//
LOCUS       DATNAE010000011         2805 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_309528, whole genome shotgun sequence.
ACCESSION   DATNAE010000011 DATNAE010000000
VERSION     DATNAE010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309528"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..710
                     /gene="efp"
                     /locus_tag="VN518_00220"
     CDS             147..710
                     /gene="efp"
                     /locus_tag="VN518_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011582497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HXE03028.1"
                     /translation="MKINGNEIRPGNVIEHKGGLWVAVKTQAVKPGKGPAYAQVELKN
                     AIDGTKLNERFRSSETVERVRLEQKDYQFLFADGDMLTFMDSDTYDQIQIPRDLLEER
                     AAFLQDGMKVTVESHEGRPIGVAIPDQVTLQVVEAEPVVKGQTAASSYKPAKLENGVR
                     VLVPPFVGVGERVVVDTNEITYVRRAE"
     gene            823..1644
                     /locus_tag="VN518_00225"
     CDS             823..1644
                     /locus_tag="VN518_00225"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HXE03029.1"
                     /translation="MPASALMTVMLAAARKAGRSLARDFGEVEQLQVSLKGPANFVSA
                     ADTRAEEILFTELSHARPGYGFLMEERGEIKGADNTHRWIVDPLDGTTNFLHGIPIFA
                     ISIALEREGELVAGLIFNPANQETFTAERGKGAYLNDRRRLRVAARTELATSVVATGI
                     PHSGRPEHALFLQELQAVMASSAGVRRMGAAALDLAWTAAGRLDGFWERNLKPWDLAA
                     GIVILREAGGYVSDADGKDGMLAKGSVVAGNEAIQRHLLKLLKTAASEPAAAAST"
     gene            1764..2795
                     /locus_tag="VN518_00230"
     CDS             1764..2795
                     /locus_tag="VN518_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotA"
                     /protein_id="HXE03030.1"
                     /translation="MAPVTYKVKLTRPQVFLWRMSLFLILAILLAIILDGQTQQLRHS
                     FLANPGLNALIIGVLVIGIVYAFRQVLRLYPEITWVNNFRISDPGITTDKTTPTLLAP
                     MATMLKDRTGHLSLATGATRSLLDSVASRLDEQRETTRYLVGLLVFLGLLGTFWGLLQ
                     TVSSVGATIGALDTNAGESVMLFDQLKEGLSGPLKGMGTAFSCSMFGLAGSLILGFLD
                     LQAGHAQGRFYNELEDWLSGITELQPADAATAGAVAPQLRFALMDMQRSLSDLSEKVE
                     LRVLNDNTSDAVKDLAQGVDKLIRQMRAEQKVVREWADEQAQQQQELATVLRQITDKS
                     FMPAREERS"
BASE COUNT          517 a          912 c          906 g          470 t
ORIGIN      
        1 agctcaccaa ggagcagcgg gcaaaagccg agaaagaggc cgaggagtgg cgcgaggacc
       61 acttgggcga ctagacgccc gcacttgaca ggtgcaagcc ggcgcgtgct taaaggcgcg
      121 ggaacccaaa ttttccaagg cattccatga agatcaacgg caacgagatc aggcccggaa
      181 acgtcatcga gcacaaaggt ggtctttggg tcgcggtgaa gacgcaggcc gtgaagcccg
      241 gcaagggccc ggcctatgcg caggtcgagc tgaagaacgc catcgacggc accaagctca
      301 atgagcgctt ccgctcctcg gagacagtcg agcgcgtgcg cctggaacag aaggattatc
      361 agttcctctt cgccgacggc gacatgctca ccttcatgga ttcggacacc tacgaccaga
      421 tccagatccc gcgcgacctg ctggaggagc gcgccgcctt tctgcaggac ggcatgaagg
      481 tgacggtcga gagccatgag gggcgcccta tcggcgtcgc catccccgac caggtgacgc
      541 tgcaagtggt cgaggccgag ccggtggtga agggccagac cgcggcctcg tcctacaagc
      601 cggccaagct ggaaaacggc gtgcgcgtgc tggtgccgcc gtttgtcggg gtgggcgagc
      661 gcgtggtggt cgacaccaac gagatcacct acgtccgccg cgccgaataa acgcagcagt
      721 ttccgtcata ccggcgaaag ccggtatcca gacaaacggc tcggagcaag cagccctgga
      781 ttccggcttg cgccggaatg acgcggtgca aggaaaagca gcatgcccgc atcggccttg
      841 atgactgtga tgctcgctgc cgcccgcaag gccggccgca gccttgcgcg cgatttcggc
      901 gaagtggagc agctccaggt ctcgctcaaa gggccggcca atttcgtcag cgccgccgac
      961 acccgcgccg aggagattct ctttaccgag ctgtcgcacg cgcgccccgg ctacggcttc
     1021 ctcatggagg agcgcggcga gatcaagggc gccgacaaca cgcatcgctg gatcgtcgat
     1081 ccgctcgatg gcaccaccaa cttcctgcac ggcatcccga tcttcgccat ctccatcgcg
     1141 ctcgagcgcg agggcgagct ggtggcggga ctgatcttca atcccgccaa tcaggagacc
     1201 ttcaccgccg agcggggcaa aggcgcctat ctcaacgacc gccgccggct acgggtcgcc
     1261 gccaggaccg agctcgcgac ctcggtggtc gccaccggca tcccgcatag cggccggccc
     1321 gagcacgcgt tgttcctgca agagcttcag gcggtgatgg caagctcggc cggggtgcgc
     1381 cgcatgggcg cggcggcgct cgaccttgct tggaccgcgg ccggccggct cgacggcttc
     1441 tgggagcgca acctcaagcc ttgggatctc gccgccggca tcgtcatcct gcgcgaggcg
     1501 ggaggctatg tcagcgacgc cgacggcaag gatgggatgc tggcgaaggg cagcgtggtg
     1561 gccggcaacg aggcgatcca aagacacctg ctcaagctgc tcaagaccgc cgccagcgag
     1621 ccggcggcgg cggcgtcgac ctgaggctgg gcgtttttta cagccgtgga cgggaaacgt
     1681 ccgccctgag gttgccgttt tgcgtgtcat agtattcggt tagtcttgga ttcgcttggt
     1741 ccgtccggct agaacggcat ctgatggcgc ctgtcactta caaggtgaag ctcacccgcc
     1801 cgcaggtttt cctgtggcgc atgagcctct tcctcatcct cgccatcctg ctggccatca
     1861 tcctcgacgg ccagacgcag cagctgcggc acagcttcct ggccaatccc gggctgaatg
     1921 cgctgatcat cggcgtgctc gtgatcggca tcgtctatgc gttccgccag gtgctgcggc
     1981 tctacccgga gatcacctgg gtcaacaatt tccgcatctc cgatcccggc atcaccaccg
     2041 acaagaccac gcccaccctg ctcgcgccca tggcgaccat gctcaaggac cgcaccgggc
     2101 atttgtcgct cgccaccggc gcgacccgct cgctgctcga ttcggtcgcg tctcgtctcg
     2161 acgagcagcg cgagacgacg cgctatctgg tcgggcttct cgtctttctc ggcctgctcg
     2221 gcaccttctg gggattgctg caaacggtga gctcggtggg cgccaccatc ggcgcgctgg
     2281 acaccaatgc cggcgagagc gtgatgctgt tcgaccaatt gaaggaaggc ctctccggtc
     2341 cgctcaaggg catgggcacg gccttctcct gctcgatgtt cggtcttgcc ggctctctca
     2401 ttctcggctt tctcgacctt caagcgggcc acgcgcaggg acgcttctac aacgagctcg
     2461 aggattggct gtcgggcatc accgagctcc agccggccga tgccgcaacg gcgggagccg
     2521 tggcgccgca actcagattc gcgctcatgg atatgcagcg ctcgctgtcg gatctgagcg
     2581 agaaggtcga gctcagagtt ctgaacgaca ataccagcga cgcggtgaag gatttggcgc
     2641 aaggcgtcga caagctcatc cgccagatgc gcgcagagca gaaagtggtg cgcgaatggg
     2701 ccgacgagca ggcccagcag cagcaggagc ttgccaccgt gctccgccag atcacggaca
     2761 agtcgttcat gccggcgcgg gaggagcgga gctagatggc tctcg
//
LOCUS       DATNAE010000012         2160 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153 k141_8205,
            whole genome shotgun sequence.
ACCESSION   DATNAE010000012 DATNAE010000000
VERSION     DATNAE010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8205"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VN518_00235"
     CDS             <1..241
                     /locus_tag="VN518_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009451034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HXE03031.1"
                     /translation="SLEPGGILRLGSGESVTLLPGVWHAFWAEEKDVLIGEVSTVNDD
                     LTDNVFAEPVSRFSDIEEDEEPLHLLVSDYEKWLV"
     gene            281..913
                     /locus_tag="VN518_00240"
     CDS             281..913
                     /locus_tag="VN518_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate hydrolase"
                     /protein_id="HXE03032.1"
                     /translation="MSEVASIAAALFRHVPHGKRVLVAIAGAPGSGKSTLAAGLAEAL
                     TAGGHSAVAVPVDGFHYDDAVLDRIGLRSRKGAPETFDFAGFESLLARLRRGDADVAI
                     PLFDRSMELARSAAAIVPAGTRFILVEGNYLLLDEAPWSRLRPLFDFAVFLDVPREEL
                     ERRLLQRWVDHGRADGPAWVASNDMPNVDRVLAKRLPANLVVQAEVRDAA"
     gene            complement(938..1708)
                     /locus_tag="VN518_00245"
     CDS             complement(938..1708)
                     /locus_tag="VN518_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015314496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase and adducin N-terminal
                     domain-containing protein"
                     /protein_id="HXE03033.1"
                     /translation="MSIAHLAAERRSNLPYYEERVDLAAAFRWTARLNMHEAVANHFS
                     LAVDADGTRFLMNPNLMHFSRIRASDLLLIDANDPATMERPDAPDPTAWGLHGAIHRH
                     CDHARCVMHVHSVHATVLASLADSTLPPIDQNSAMFFKRHVVDTDYGGLAFEEEGERC
                     ATLLADPKVKVMVMGNHGVLVIGDTVADTFDRLYYFERACETYIKALWTGRPLRLLPD
                     AVADKVGIEMDNYPVPQASRHLGELKLILDEEGSTYRD"
BASE COUNT          359 a          723 c          693 g          385 t
ORIGIN      
        1 aagcctggag ccgggcggca tcctgcgcct cggttccggc gaaagcgtca cgctcctgcc
       61 cggcgtttgg cacgccttct gggccgagga gaaggacgtg ctgatcggcg aggtctcgac
      121 ggtcaacgac gatctgacgg acaatgtctt cgccgagccg gtcagccgct tttccgatat
      181 cgaggaggac gaggagccgc tccatctgct tgtgtccgac tatgaaaaat ggctcgtttg
      241 acctagccgc gttcaggcga gccttcgcga ggaggagaaa atgtccgaag tcgccagcat
      301 cgccgccgcc cttttccggc atgtgccaca cggcaagcgc gtgctcgtcg ccatcgccgg
      361 cgcgccgggc tccggcaagt cgacgcttgc cgccgggctc gccgaggcgc tgaccgccgg
      421 cggccacagc gcggtcgccg tgcccgtgga tggcttccac tacgacgacg ccgttctcga
      481 tcggatcggc ttgcgctcgc gcaagggcgc accggagacc ttcgactttg ccggcttcga
      541 gagcctgctg gccaggctgc ggcggggcga cgccgatgtc gccatcccgc tcttcgaccg
      601 ctcgatggaa ctcgcccgct ccgccgcggc gatcgtgccg gccggcacgc ggttcatcct
      661 cgtggagggc aactacctcc tgctcgacga ggcgccctgg tcgaggctcc ggccgctttt
      721 cgatttcgcc gtcttcctcg acgtgccacg cgaagaactc gagcgccggc ttttgcagcg
      781 ctgggtcgac catggccgcg ccgacggtcc ggcctgggtc gcctccaacg acatgcccaa
      841 tgtcgaccgc gtcctggcga agcggctccc ggccaacctc gtggtgcagg ccgaggtgcg
      901 cgacgccgcc tgagcgcccg cgctcggcaa accgcgctca gtcgcgatag gtcgagcctt
      961 cctcgtcgag gatgagcttg agctcgccga gatgcctgga cgcctgcggc acgggatagt
     1021 tgtccatctc gatgccgacc ttatccgcca ccgcgtcggg cagcaggcgc agcggcctgc
     1081 cggtccacag cgccttgatg taggtctcgc aggcgcgctc gaaatagtag agccggtcga
     1141 acgtgtcggc gaccgtgtcg ccgatgacca gcacgccgtg attgcccatc accatcacct
     1201 tgaccttggg atcggcgaga agcgtcgcgc accgctcgcc ttcctcctcg aaggcgagcc
     1261 cgccatagtc cgtatcgacc acatggcgct tgaagaacat cgccgaattc tggtcgatcg
     1321 gaggcagagt ggaatccgcc agcgaggcga gcaccgtggc gtggaccgaa tgcacatgca
     1381 tgacgcagcg cgcatgatcg caatggcggt ggatcgcgcc gtgcaggccc caggcggtcg
     1441 gatcgggcgc gtccggccgc tccatcgtcg ccgggtcgtt ggcgtcgatc aacaggagat
     1501 cgctcgccct tatgcgcgag aagtgcatca ggttcgggtt cattaggaac cgcgtgccgt
     1561 ccgcgtcgac ggcgagcgag aaatggttgg ccaccgcctc gtgcatgttg agcctcgccg
     1621 tccagcggaa ggcggccgcg agatctaccc gttcctcgta gtagggcagg ttggagcggc
     1681 gttcggcggc gagatgcgcg atgctcatga cggtcctcct tctctctcgg gcagcttgcc
     1741 gctttcgtgg ccgcgcttca accgatatgc gacgaggatg ttcatcggaa tggatcttct
     1801 tcgaatccgg gatcacgaaa ggcccagcgg caagcaacat actgtaacga ttggtgaaaa
     1861 tactgccgca ttgcgcattt ccgtcatcat atgatcatcg aatgacccga tcgtggccgg
     1921 gttgccatca cattgcggca ttgaactcga cgcctcgccc ccgaaggcga gtcccccaaa
     1981 aattcttgcc aatgatggaa aggcagaacc atgttgaaga ccgtatttct cgcatccgcg
     2041 atgaccctgg ccctctcggg cgtcgccggc gcgcagcagc agccggctcc ttccagcccg
     2101 gctccggcgc agcaggcgcc aagcgccacg gcgccgaaga tcagcgcggt caatgtcgtc
//
LOCUS       DATNAE010000013         2693 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_911356, whole genome shotgun sequence.
ACCESSION   DATNAE010000013 DATNAE010000000
VERSION     DATNAE010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_911356"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="VN518_00250"
     CDS             <1..290
                     /locus_tag="VN518_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03034.1"
                     /translation="QSKTILRPGDLISFSGTAWDPNNNPIRWQVRIVGRPFNEYQTLD
                     ALTNWEWRVEERDIGEPAMIEIGVISSKPYHRYGNRDDYISIMYQVLPPRL"
     gene            453..>2693
                     /locus_tag="VN518_00255"
     CDS             453..>2693
                     /locus_tag="VN518_00255"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HXE03035.1"
                     /translation="MDDLLREFLTETNESLAVLDLELVKLEQNPEDPKLLGSIFRIVH
                     TIKGTCGFLGLARLEGVAHASESVLGKIRDGELAVSAGAIDLVLESLDRIKSLLAALE
                     ESEVEPAGDDRDLIDRLTQLAEGGAPAPSAAAPAPKPVAPPKPVEIPKPAAKDPAAED
                     EEPPLTDEDIERILAEPMNPPAAAPVEEAQEGLPEPVTTGRKRTAATIIASRPRAPAE
                     DVHEAEPTPAPARASKPAPTPAPAASAPAVAAPAGEAPEAKESAVAAQSIRVHVDLLE
                     NLMTMVSELVLTRNQLMQILRAHKDTDFAAPLQRLSHVTTELQEGVMKTRMQPIGNAW
                     AKLPRIIRDLSHELHKKIDLQMLGSETELDRQVLEMIRDPLTHMVRNSADHGIELPAE
                     RLRAGKPETGTITLNAYHEGGHIIIEISDDGRGLDTERIKRKAAMMGLASEADLQAMS
                     EQQIHQYIFKPGFSTATTVTSVSGRGVGMDVVRSNMEKIGGTVEMKSHHGKGSLFIIK
                     IPLTLAIVSALIVECGSERFAIPQLSVVELVRAAVKADTSIEWLNNAPVLRLRDRLLP
                     LVSLHELLKLGRRPDHPTETFIVVTQVGTLTFGIIVDRVYDTEEIVVKPVAPILRDIP
                     IFSGNTILGDGSVIMILDPNGIATSAGEVTVGSGARTDHAPARLAQRDDQTSLLLFRA
                     GSATPKAVQLSLIARLEEIDLAKIERSNDRPVVQYRGKLMPIVTLDPSHELPKEGRRP
                     ILVFAER"
BASE COUNT          533 a          927 c          827 g          406 t
ORIGIN      
        1 tgcaatccaa aacgatattg agaccgggtg acctcatttc tttctctggg acggcatggg
       61 atccgaacaa caacccgatc aggtggcaag tgcgtatcgt tggaaggcct ttcaatgagt
      121 atcagacact tgatgcgttg accaactggg aatggcgcgt ggaggagcgt gacatcggcg
      181 agccggcgat gatcgagata ggcgttattt catcgaagcc atatcacagg tacggtaatc
      241 gcgacgacta catctcgata atgtatcagg tactgccacc ccgcctataa ctaactccac
      301 gaagttaacc actttctcac aaagtattcc aagagattaa caaccgtcat cgctaactgc
      361 ttcttaaagt tttttcgaca agttttgggc caatctcctg ggccgccttt cgcggccaag
      421 gcacggcgcg acgcacacgc caaggaacgg ccatggacga cctgctccgc gaattcttga
      481 ccgagacgaa tgagagcctc gcggtgctgg acctcgagct tgtcaagctc gagcagaatc
      541 cggaagaccc caagctgctc ggcagcatct tccgtatcgt ccacaccatc aagggaactt
      601 gcggctttct cgggctggcg cgcctcgagg gcgtggcgca tgcctcggag agcgtgctcg
      661 gcaagatccg cgacggcgag ctggcggtgt ccgcgggcgc gatcgacctg gtgctggaat
      721 cgctcgaccg catcaagtcg ctgctcgcag cgctcgagga gagcgaagtc gagccggcgg
      781 gcgatgacag agatctgatc gatcgcctga cgcagctcgc cgaaggcggc gcgccggcgc
      841 caagcgctgc cgcgccggcg cccaagccgg ttgcgccacc caaaccggtc gagataccga
      901 agccggcagc gaaagacccg gctgccgagg acgaggagcc gccgctcacc gacgaggata
      961 tcgagcgcat cctcgccgag ccgatgaacc cgcccgcggc ggcgccggtc gaggaagcgc
     1021 aggaaggcct gcccgagccg gtcaccaccg gacgcaagcg caccgccgcg acgatcatcg
     1081 cctcgcggcc gcgcgcgccc gccgaggatg tccacgaggc tgaacccaca ccggcgccgg
     1141 cgcgcgcatc gaagccggcg ccgacaccgg ctccggccgc gtccgctccg gccgtggcgg
     1201 cacccgccgg cgaggcgccc gaggccaagg aatcggcggt cgcggcgcaa agcatccgcg
     1261 tccatgtcga cctcctcgag aatctgatga ccatggtcag cgagctggtg ttgacccgca
     1321 accagctcat gcagatcctg cgcgcgcaca aggacaccga tttcgccgcg ccgctgcagc
     1381 gcctctcgca tgtcacgacc gagctgcagg aaggcgtcat gaagacgcgc atgcagccga
     1441 tcggcaacgc ctgggccaag ctgccgcgca tcatccgcga cctgtcgcac gagctgcaca
     1501 agaagatcga tctgcagatg ctgggctccg agaccgagct cgaccgccag gtcctcgaga
     1561 tgatccgcga tccgctgacg catatggtgc gcaactccgc cgatcacggc atcgagctgc
     1621 ccgccgagcg cctgcgcgcc ggcaagcccg agaccggcac gatcacgctc aacgcctatc
     1681 acgagggcgg ccacatcatc atcgagatca gcgatgacgg ccgcggcctc gacaccgagc
     1741 gcatcaagcg caaggcggcg atgatgggcc tcgccagcga ggccgatctg caagccatga
     1801 gcgagcagca gatccaccaa tacatcttca agccgggctt ctcgacggcc accaccgtca
     1861 ccagcgtctc gggccgcggc gtcggcatgg acgtcgtgcg cagcaacatg gagaagatcg
     1921 gcggcacggt ggaaatgaag agccatcacg gcaagggctc gctcttcatc atcaagattc
     1981 cgctcaccct cgccatcgtc tcggcgctca tcgtcgaatg cggcagcgag cgcttcgcca
     2041 taccgcagct cagcgtggtc gagctggtgc gcgccgccgt caaggccgac accagcatcg
     2101 aatggctcaa caatgcgccg gtgctgcgcc tgcgcgaccg cctcttgccg ctcgtctcgc
     2161 tgcacgagct gttgaagctc ggccgccggc cggatcaccc gaccgagacc ttcatcgtcg
     2221 tgacgcaggt cggcacgctc accttcggca tcatcgtcga ccgggtctat gacacggagg
     2281 agatcgtggt gaagccggtc gcgccgatcc tgcgcgacat cccgatcttc tcgggcaaca
     2341 ccatcctcgg cgacggcagc gtcatcatga tcctcgatcc caacgggatc gccaccagcg
     2401 ccggcgaggt caccgtcggc agcggtgcgc gcaccgatca cgcgccggcg cgcctggcgc
     2461 agcgcgacga ccagaccagc ctgttgctgt tccgcgccgg cagcgccacg cccaaggcgg
     2521 tgcagctctc gctcatcgcc cgactggagg agatcgacct cgccaagatc gagcgctcga
     2581 acgaccggcc agtcgtgcag tatcgcggca agctcatgcc gatcgtcacg ctcgacccga
     2641 gccatgagct gcccaaggaa ggccgccggc cgatcctggt cttcgccgag cgc
//
LOCUS       DATNAE010000014         2784 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_1814408, whole genome shotgun sequence.
ACCESSION   DATNAE010000014 DATNAE010000000
VERSION     DATNAE010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1814408"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(350..565)
                     /locus_tag="VN518_00260"
     CDS             complement(350..565)
                     /locus_tag="VN518_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03036.1"
                     /translation="MKRQFAEHARDHALKAISELSALLVAGESEMPKPEFQALKQAVA
                     SIIGLIERDVLSSIYAKHGDLDDLGRP"
     gene            complement(1304..2017)
                     /locus_tag="VN518_00265"
     CDS             complement(1304..2017)
                     /locus_tag="VN518_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012320824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXE03037.1"
                     /translation="MTSTGRLTGRIAVITGASRGIGAAVAKRFAAEGAQVIALARTVG
                     GLEELDDAIRAGGGRATLVPLDLRDFDKIDQLGATLHERFGRLDVLVGNAGVLGSLSP
                     MGHFKPKIWAEVIETNLTANWRLIRSLDPLLRLSEAGRAIFTTCAAARDATPYWGAYA
                     ASKAALEAMVKIYAGELGQTRVRANLVDPGIVATKLRTQGFPGEDQARLAVPGDVTEP
                     FVALAAADCRENGEVVGVC"
     gene            complement(2171..>2784)
                     /locus_tag="VN518_00270"
     CDS             complement(2171..>2784)
                     /locus_tag="VN518_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HXE03038.1"
                     /translation="ARESHIPADTVIPVPDSGVPAALGYAQESKLPFELGIIRNHYVG
                     RTFIEPTDQIRHLGVKLKHNANRAKLQGKRVILVDDSIVRGTTSTKIVEMVRQAGALE
                     VHMRISSPPTSHSCFYGIATPERDKLLASHMDVQEMARFIGADTLAFISIDGLYRAMG
                     ETRRDPAAPKFCDACFTGEYPIALPDHDAGKVSTQLSLLAETA"
BASE COUNT          485 a          838 c          928 g          533 t
ORIGIN      
        1 attgcggctc ggcattgtcg atgtgaagcg cggcgacgat ctcggcttcg tcgtagagct
       61 ccttgagaga aagcgtatag acgaaattcg ccgccgaggg cgccgccggc agaacgacac
      121 cggcgatcac gagcgcgaag cggagccagt ggccgacggc gcgccgcatt ttgcctgcct
      181 agtccttcgc gtaggttctg aactctttgg gaattggaat accggtgatg cgcgacgcga
      241 tctcattcgc atcgcgcttg cagtcttttg gcgggctctg gaggctgtgc aagggggaag
      301 aatccttctg cgggagagga tgcttgactc tctgggctta ccgacttcgt cagggccgtc
      361 ccaagtcatc gagatcgccg tgcttagcat aaatactaga caacacatct ctttcgatga
      421 ggccgataat tgacgcaacg gcttgcttga gggcttggaa ttctggcttc ggcatttcgc
      481 tctcgccggc gacaaggagg gcggaaagct ctgaaatcgc tttcaacgcg tggtctcggg
      541 cgtgctcggc gaattgtctc ttcaagtcaa cctccccatg cattcgcgat agcatcgccg
      601 gaattccgag gattgcagat ccccagaagg cgaaggcaac agatgaaggc actgctgaac
      661 acatcgatcg tgctttgagg ctggtcccgg cagcacgtcg gagacgacac tggtgtcgtt
      721 gcctgggtgc ggcatcggat tggcatcgac cgaaccggct gggcgaaatc gaccaccctg
      781 gtggccagcc gcaccggctg gccaccgcgg atgttggtct ggatcaaatc cctttagcag
      841 actgatgctt cccaggtgag ccgcgagtgt catcaatccc gcatcgcccg ccctcggcga
      901 agtgttcggg gtgatgagat gcagcgtcat cagtttgcca ttgcgagatc attccggttc
      961 agacgctctg cgatccgacc gagcgtctcc gcctttgatg cgacgtcgac ggcaatgttg
     1021 taggccttgg ttagcgtctc actgagctca ttgggcgctc gaacggtcca aacgctctgc
     1081 ccgaggcgcg tttttgcgcg ctccagcatt ggtgtgaatc caaaagtgag cccatccacc
     1141 ccgcagctca caccgggttc gccgtgcctg tcggcaagcc gaacccacat accgccttgc
     1201 attggaatct ccatcgccct ccgggagaac aaatagcgaa ctatgcctat gcgctcaaga
     1261 gcgctttatg tgagatgttt cccgcggccg gcgttggggt ctgctagcaa acgccgacaa
     1321 cctcgccgtt ctctcggcaa tccgccgcgg ccagcgcaac gaacggctcc gtcacatccc
     1381 cgggcaccgc cagccgcgcc tggtcctcgc cggggaagcc ctgggtgcgc agcttggtgg
     1441 cgacgatgcc gggatcgacg agattggcgc ggaccctggt ctggccgagc tcgccggcat
     1501 agattttgac catggcctcg agcgccgctt tgctcgcggc ataggcgccc caatagggcg
     1561 tcgcatcgcg cgccgcggcg caggtggtga agatggcgcg gccggcttcc gacaggcgca
     1621 gcagcgggtc cagcgagcgg atgaggcgcc aattggcggt gaggttggtc tcgatcactt
     1681 cggcccaaat cttcggcttg aaatggccca tgggcgagag cgagccgagc acgccggcat
     1741 tgccgaccag cacatcgagg cggccgaagc gctcatgaag ggtggcaccg agctggtcga
     1801 tcttgtcgaa gtcgcggagg tcgagcggca ccagcgtcgc gcgtccgccg ccggcgcgga
     1861 tggcgtcgtc gagctcctcc agcccgccca ccgtgcgcgc cagcgcgatc acctgagcgc
     1921 cctcggcggc aaagcgcttc gcgaccgccg caccaatgcc gcgcgacgcg ccggtgatga
     1981 cggcgatgcg gccggtgagg cgtccggtgc ttgtcattgg ttcagcgctg caatttggtg
     2041 gttgcgtcct tcgacaagct caggatgagg ccgagcggga atggcatcgt cggttgatgc
     2101 cgcactctgg agtccctcat cctgagcttg tcgaaggacg caccatcggc ttgcagcctg
     2161 taacaggccc tcacgccgtc tcggcgagga gcgagagttg tgtcgagacc ttgccggcgt
     2221 cgtggtcggg cagcgcgatc ggatactcgc cggtgaagca ggcgtcgcag aatttgggcg
     2281 ccgccggatc gcggcgcgtc tcgcccatgg cgcggtagag cccgtcgatc gagatgaagg
     2341 caagagtgtc ggcgccgatg aagcgtgcca tctcctgcac gtccatatgc gaggcgagga
     2401 gcttgtcgcg ctcgggcgtg gcgatgccgt agaagcagga atggctggtg ggcgggctcg
     2461 atatgcgcat atgcacctcg agcgcgccgg cctggcgcac catctcgacg atcttggtcg
     2521 aggtcgtgcc gcgcacgatc gaatcgtcga cgagaatgac gcgcttgccc tggagcttgg
     2581 cgcggttggc gttgtgcttg agcttgacgc cgagatggcg gatctggtcg gtcggctcga
     2641 tgaaggtgcg gccgacgtaa tggttgcgga tgatgccgag ctcgaagggc agcttgcttt
     2701 cctgcgcgta gcccaacgcc gccggtacgc ccgaatcggg tacggggatg acggtatcgg
     2761 cggggatgtg gctctcgcgc gcga
//
LOCUS       DATNAE010000015         2337 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2720798, whole genome shotgun sequence.
ACCESSION   DATNAE010000015 DATNAE010000000
VERSION     DATNAE010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2720798"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VN518_00275"
     CDS             <1..495
                     /locus_tag="VN518_00275"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HXE03039.1"
                     /translation="VTAVKEGADGGAMFEVDVSNETAARTTLGRWKQGSRINLERPLT
                     FGSELGGHLMTGHIDGLARVTEMTEDDHSLRFVLDAPQEFAPFIAPKGSVALNGVSLT
                     VNEVDGTRFGVNIIPYTLTHTTWGDRKPGDLINLEVDLLARYVERIAAGRLSASRAAL
                     EQDR"
     gene            499..1626
                     /gene="ribB"
                     /locus_tag="VN518_00280"
     CDS             499..1626
                     /gene="ribB"
                     /locus_tag="VN518_00280"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HXE03040.1"
                     /translation="MNIVSQTRYHPALSPIEEVIEDARNGRMFILVDAEERENEGDLV
                     VPAQMATPDAINFMTKHGRGLVCLSLNADRAKQLHLELMSRSNRARHSTAFTVSIEAR
                     EGVSTGISAHDRAHTISVAIDPTKDFREIMTPGHVFPLVAKDGGVLVRAGHTEAAVDI
                     ARLAGLYPAGVICEIMNDDGTMARLPDLVAFAQRHGLKIATIAELIAYRLRYDHLVKV
                     VAETTITSANGGSFALKVYATTVDPAEHVALVKGDVSDGGPVLARVHATNTLDDILGA
                     GGHRETLVHKAMDIIGREGRGVIVLIRESRPDAISSRLTREEKPEQSGQQRLIEYGIG
                     AQILLDLGVHEMVLLSNSPARKIVGLEGYGLKVVGHRSLAS"
     gene            1623..2093
                     /gene="ribH"
                     /locus_tag="VN518_00285"
     CDS             1623..2093
                     /gene="ribH"
                     /locus_tag="VN518_00285"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HXE03041.1"
                     /translation="MSRKSPRSVLIIESRFYEDIADELARGAVDELEGRHIEYERIAV
                     PGSLEIPLALSLALSTGFVGRAGRHRGCIALGCVIRGETTHFDIVARESAAGLHALAV
                     EHAVPLGNGILTCDSRAQALARASVTGRNKGGDAARACLALMQIEQNFSSASRR"
     gene            2093..>2337
                     /locus_tag="VN518_00290"
     CDS             2093..>2337
                     /locus_tag="VN518_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HXE03042.1"
                     /translation="MTGSGQKKPASGRSPQARTAARLAAVQALYQMDMTAIDLNHVIA
                     EFEVHRLGQEVEGNQYCDAETQFFRDIVEGVVREQRR"
BASE COUNT          421 a          798 c          727 g          391 t
ORIGIN      
        1 gtgacggccg tcaaggaggg cgccgatggc ggcgccatgt tcgaggtcga tgtctccaat
       61 gagaccgccg cccgcaccac gctagggcgt tggaaacagg ggagccgcat caatctcgag
      121 cgcccgctga ctttcggctc cgagctcggc ggccatctga tgaccggtca catcgacgga
      181 ttggcccgcg tgacggaaat gacggaggat gatcacagcc tcaggttcgt gctcgacgcg
      241 cctcaggaat tcgccccgtt tatcgcgccc aaaggctcgg tggcgctcaa cggcgtgtcg
      301 ctcacggtaa acgaggtaga cggcacgcgc ttcggcgtga acattatccc ctacaccttg
      361 acccacacga cgtggggaga ccgcaagcca ggcgatctga taaaccttga ggtggatttg
      421 ctcgcgcgct acgtcgagcg gatcgcggcg ggtcgcctct cggcaagccg cgccgcattg
      481 gaacaggatc ggtaacacgt gaatattgtg agccaaacac gctatcaccc ggcgctctcg
      541 cccatcgagg aggtcatcga ggacgcgcgc aacgggcgca tgttcattct cgtcgatgcc
      601 gaggagcgcg agaacgaagg cgacctcgtc gttccagcgc agatggcgac gcccgacgcc
      661 atcaacttca tgaccaagca tggccgcggg ctcgtctgcc tgtcgctcaa cgccgatcgc
      721 gccaagcagc ttcatcttga gctgatgtcg cgctccaacc gcgcccgcca ctccaccgcc
      781 ttcaccgtgt cgatcgaggc gcgcgagggc gtctcgaccg gcatctcggc gcatgaccgc
      841 gcccacacca tctccgtcgc catcgacccc accaaggact tccgggagat catgacgccg
      901 ggccatgttt tcccgctggt ggccaaggac ggcggcgtgc tcgtgcgcgc cggccacacc
      961 gaggccgccg tggacatcgc ccggctcgcc ggcctctatc cggcaggcgt gatctgcgag
     1021 atcatgaacg atgacggcac catggcgcgt ctgcccgacc tcgttgcctt cgcccagcgt
     1081 catgggctca agatcgccac catcgccgaa ctgatcgcct atcggctccg ctacgatcat
     1141 ctggtcaagg tcgttgccga gaccaccatc acaagcgcca atggcggcag tttcgcgctc
     1201 aaggtctatg ccaccaccgt cgatcccgcc gagcacgtgg cgctcgtgaa gggcgatgtg
     1261 agtgacggcg gcccagtgct cgcccgcgtg catgccacca acacgctcga cgatattttg
     1321 ggcgcaggcg gccatcgcga gactctcgtg cacaaggcca tggacattat cggccgcgag
     1381 ggtcgcggcg tgatcgtgct gatccgcgaa agtaggcctg acgccatctc ttcccgtctc
     1441 acccgcgaag agaagcctga gcagagcggc cagcagcggc tgatcgaata cggcatcggc
     1501 gcccagatcc tgctcgatct cggggtgcac gagatggtgc tgttgagcaa ttcaccggcg
     1561 cgcaagatcg tcggccttga gggctacggg ctcaaggtcg tcggccatcg gagcctcgcc
     1621 tcatgagccg caaatctccg cgctcggtgc tgatcatcga gtcccgcttc tacgaggaca
     1681 ttgccgatga gctcgcgcgc ggcgcggtcg acgagcttga aggccgccac atcgagtatg
     1741 agcggatcgc cgtgcctggc tcgctcgaga tcccgcttgc attgagcctc gcgctttcca
     1801 ccggcttcgt cggccgggca ggccgccatc gcggctgcat cgcccttggc tgcgtcatcc
     1861 gcggcgagac cacccatttc gacatcgtgg cgcgcgaatc cgccgccggc ttgcacgcgc
     1921 ttgccgtcga gcacgccgtg ccgctcggca acggcatcct cacctgcgac agccgcgcgc
     1981 aggcgctcgc cagggcgagc gtcaccgggc gcaacaaagg cggcgacgcc gcccgcgcct
     2041 gtctcgcgct gatgcagatc gagcagaatt tctcctcggc gagccgacgc tgatgaccgg
     2101 ttcaggccaa aagaagcctg cttcgggacg aagtccccaa gcacggaccg cggcacgact
     2161 cgctgccgtc caggcgctct accagatgga catgaccgcc atcgacctca accatgtgat
     2221 cgccgagttc gaggtgcacc ggctcggcca ggaagtcgaa ggcaaccaat attgcgatgc
     2281 cgagacgcag ttcttccgcg acattgtcga gggcgtggtg cgcgagcagc gccgtat
//
LOCUS       DATNAE010000016         2773 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2422683, whole genome shotgun sequence.
ACCESSION   DATNAE010000016 DATNAE010000000
VERSION     DATNAE010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2422683"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VN518_00295"
     CDS             <1..219
                     /locus_tag="VN518_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03043.1"
                     /translation="RADSGGGALGLRIFEGACIGCHSFDGSGAVSKYATLLGTRAVND
                     PAGTNATQALLQGTHVHSSPHPCPMRDA"
     gene            954..1961
                     /locus_tag="VN518_00300"
     CDS             954..1961
                     /locus_tag="VN518_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012494547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HXE03044.1"
                     /translation="MAVSLKRDRFPVDDAREAIASAARTVQLEAQGLRALEAALDKEL
                     VDSFAEAIRLVSACRGRVIVTGMGKSGHIGMKVAGTLSSTGTPASFVHPSEASHGDLG
                     MITRNDVILAFSWSGETVELGNLVSYSRRFAVPLIAVTSNPDSTLAKAAEVVLALPHS
                     KEACPHGLAPTTSTTMQLALGDCLAIALLESKGFTARDFQVLHPGGQLGARLKLVGDL
                     MHKGDRLPLTPGDMKMAEAIVVMTEKAFGCLGVVDEDGRLAGIITDGDLRRNMGNGLL
                     SRHTGEIMTAGPKTVSPELLASAALEMINSSSITALFVVEDGRPVGIIHIHDLLRAGV
                     A"
     gene            1966..>2773
                     /locus_tag="VN518_00305"
     CDS             1966..>2773
                     /locus_tag="VN518_00305"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXE03045.1"
                     /translation="MVTQSFDVLVIGGGIHGAAVARDAAGRGLKVMLAEKSDYASATS
                     SASSKLIHGGLRYLEQLELALVRESLVERAGLLATAPHLVEPLRFLLPIYGWQKRPAL
                     LLHAGLALYDLLSIGDGLPPSGRLKPQEIAELPRLRRENLNAVLHYHDCQADDARLVL
                     AVLLDARARGADILNRRAVTAIEPLANGYTIELDERGAKRRVDARFIVNAAGPFVAGV
                     DALSSIAPPPRPLRLIRGSHIVLRMPEPVEADAYTLQDQEDRVIFVLPWLD"
BASE COUNT          451 a          973 c          892 g          457 t
ORIGIN      
        1 cgggccgact ccggcggagg cgcgcttggc ttgcgcatct tcgagggcgc ctgcatcggc
       61 tgccacagct tcgacggcag cggcgccgtc tccaaatatg cgacgctcct tggcacgcga
      121 gccgttaatg acccggccgg caccaatgca acgcaggccc tcctgcaggg cacgcacgtt
      181 cacagctccc cccatccctg ccccatgagg gatgcgtgac ctaggccgtc tctgtcatgt
      241 ggggttgggc cgcggcgctc gaggacaacc gcgagaggtg ggagccattt ccgagaacgg
      301 gtggcgataa gccctcgcgg ccggagcaaa cgggccacct cgagccgctt cttctcctcc
      361 gccgagctgg aaggctcagg tccatatctg cgggcctttc gccatgcgga acaggtggta
      421 agcggggaaa ggggcggaag cgatgccgca agcagggcgc cgagaggctg cccactaaaa
      481 acaggacgcg gccgcttggc gccccgcatc cctcgcttcg cgcatcaccc ggacgcttct
      541 gcgtcgcggc aacggagccg catgtctcgt catggcgcag caacagactc catacctccc
      601 ccttgcgggg aggtccggcg gcgctcttgc gacgctgggg tgggggattc ccagaaggcc
      661 acgccacgag tcccgcaaga acgtgccccc accccgacgc cgctcgcggc gtcggacctc
      721 cccacaaggg gaggtatgaa gaaggacatg aggacgtgca gtgtgcttta cccctgctcc
      781 cgtgtggcaa aggaaaggcc gcgattctca aggctttgcg ggcgttgatc ggaggcgctt
      841 cgagggacta ggcttgaccg gacccgcccc gaatcgccgc agcgttctcg gccctattcg
      901 tgcccagact gtggtagaaa agagggacgg gaaaagcggg gggaacgagg gacatggccg
      961 tcagcctcaa acgtgaccgt tttccggttg acgacgcgcg cgaggcgatc gcctcagccg
     1021 cgcgcaccgt tcagctcgaa gcgcaaggct tgcgcgcgct cgaggcggcc ctcgacaagg
     1081 agctcgtcga cagcttcgcc gaagccattc gccttgtcag cgcctgtcgc ggccgggtga
     1141 tcgtcaccgg catgggcaag agcggccata tcggcatgaa ggtggccggc acgctgtcct
     1201 cgaccggcac gccggcctct ttcgtgcatc cgagcgaggc gagccacggc gatctcggca
     1261 tgatcacccg caacgacgtg attctcgcct tctcctggtc cggcgagacc gtcgagctcg
     1321 gcaacctcgt ctcctattcg cggagattcg ccgtgccgct catcgcggtg acgtcgaacc
     1381 ccgacagcac gctcgccaag gcggccgagg tggttctggc acttccccac agcaaggaag
     1441 cctgcccgca cggccttgct cccaccacct caaccacgat gcagcttgcg ctaggcgatt
     1501 gcctcgccat cgcgctgctg gagagcaagg gcttcaccgc ccgcgacttc caggttctgc
     1561 accctggcgg ccagctcggc gccaggctga aactcgtcgg cgacctcatg cataaaggcg
     1621 atcgcctgcc gctcactccg ggcgacatga agatggccga agccatcgtg gtgatgaccg
     1681 agaaggcctt tggctgtctc ggcgtggtcg atgaggacgg caggctcgcc gggatcatca
     1741 ccgacggcga cttgcgccgg aacatgggca acgggctctt gtcgcgccac accggcgaga
     1801 tcatgaccgc cgggccaaag accgtgtcgc ccgagctgct cgcgagcgcg gcactcgaga
     1861 tgatcaactc ttcgagcatc accgcgctgt tcgtggtcga ggatgggcgc cccgtgggca
     1921 tcatccacat ccacgatctg ctccgcgccg gcgtcgccta gcaagttggt cacgcagagc
     1981 ttcgacgttc tcgtcatcgg cggcggcatt cacggcgcgg ccgtggctcg cgacgccgcc
     2041 ggccgcgggc tgaaggtgat gctcgccgag aagagcgatt atgcttccgc cacctcgtcg
     2101 gcctcgtcca agctgatcca tggcggcttg cgctatctcg agcagctcga gcttgcgctc
     2161 gtgcgcgagt ccctggtcga gcgcgctggg ctgctcgcga ccgcgccgca tctcgtcgag
     2221 ccgctccgct tcctgctgcc gatctatggc tggcagaagc gccctgccct gctgctgcat
     2281 gccggccttg ccctttacga cctcctgtcg atcggcgacg ggctgccgcc gagcgggcgc
     2341 ctcaagccgc aggagatcgc agagctccca cgtcttcgcc gcgaaaatct caacgccgtg
     2401 ctgcactatc acgactgcca ggccgacgat gcgcgcctcg tgcttgccgt gctgctcgat
     2461 gcgcgcgccc gcggcgccga tattctcaac cgccgcgcgg tgaccgccat cgagccgctc
     2521 gccaacggct atacgatcga gctcgacgag cgcggggcga agcgccgtgt cgatgcgcgc
     2581 ttcatcgtca atgccgccgg ccccttcgtc gccggggtcg acgccctgag cagcatcgcg
     2641 cctccgcccc ggcccttgcg gctgatccgc ggcagccaca tcgtcctccg aatgcctgag
     2701 ccggtcgagg ccgacgccta taccttgcag gaccaggaag accgcgtgat cttcgtgctg
     2761 ccgtggctcg acc
//
LOCUS       DATNAE010000017         3315 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_1520087, whole genome shotgun sequence.
ACCESSION   DATNAE010000017 DATNAE010000000
VERSION     DATNAE010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1520087"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="VN518_00310"
     CDS             complement(<1..422)
                     /locus_tag="VN518_00310"
                     /inference="COORDINATES: protein motif:HMM:NF045465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HXE03046.1"
                     /translation="MIDFSSASGARGSLPLLADAERLGRAIEAGAVALWEWHIPEDMM
                     QVSPRLAEQLGLVPPLLAFPASRFFALVHPNDIALLKSAMSEAIETGATFAHEFRVRR
                     EHTGELRFLCHEGRVTERDPGGAARILAGASHDVTERRL"
     gene            594..1346
                     /locus_tag="VN518_00315"
     CDS             594..1346
                     /locus_tag="VN518_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018236393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HXE03047.1"
                     /translation="MSQFAPFTYDVPCTQCPLRVCEAFRTISEDEIEFIAKLKLGELR
                     TGPATTLLREGSASEQLFTLLEGWAFRYKTLEDGRRQITSFVLPGDLVGIQSSMQGEM
                     DHSVDTLTAAVFCTFPRERVWELYKRCPSLAFDVTWLAATQEMILNENLLSIGRRNAL
                     ECAAYYLLHLYLRAADIGLVTEGKVRFPFTQEHLADALGLSLVHTNKTLRRLSARGLM
                     QWEHGWCFIPDVGALAEIAKFDLNQPRKRPLL"
     gene            1361..2704
                     /locus_tag="VN518_00320"
     CDS             1361..2704
                     /locus_tag="VN518_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXE03048.1"
                     /translation="MPPLDAPFSARLDASDLSAQWLPFTANRAFRRRPRLIDRAEGMH
                     YFAADGRNLLDAMSGLWCCNAGHCREPIVEAIRRQAGWLDYAPAFQVGHAPAFQLAAR
                     LAKMAPGDLDHVLFCNSGSEAVDSALKIALAYHKLAGASTRTRFVSRDRSYHGSCFGG
                     TSVGGIAPNRNLFEPLLDVDQIRSTYSRERQAYARGEPAWGADLADDLTAILALEERA
                     AIAAVIVEPMAGSAGVFASPQGYLQRLREITRAHGILLIFDEVITGFGRLGHAFAAER
                     YGVVPDMICFAKGVSNGAAPLGGVLVRGRIHDAFMQGPEHVPELAHGFTYSGHPLGVA
                     AASAALDLYREEGLFERAVALEPVFADAVHALKGAPHIVDIRSIGLAAGIDLAPIDGS
                     PGLRGARAFEAAFFEEDLVLRAVGDTLVLAPALIATEAEIGAITEKLRRILAALR"
     gene            2885..>3315
                     /locus_tag="VN518_00325"
     CDS             2885..>3315
                     /locus_tag="VN518_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03049.1"
                     /translation="MLDVIVLIAMAVTALAFAVGLIVQSGIAQIPALIAAAALYLVMA
                     ASYLMLARGARPTASTDRLSEVEAALEIIDKDLQRIDRVEDDVSRLDLLADRVERLDQ
                     ALSEYAATAPIGGVGRSDQVSNEFDEVHAKIDAVRADLEGEA"
BASE COUNT          516 a         1161 c         1031 g          607 t
ORIGIN      
        1 aggcggcgct cggtgacgtc gtggctcgcc ccggcaagga tcctcgccgc gccgcccggg
       61 tcgcgctcgg tcacgcgccc ctcgtggcac aggaagcgga gctcgccagt gtgctcgcgg
      121 cgaacgcgga actcgtgcgc gaacgtggcg cccgtctcga tcgcctcgct catcgccgac
      181 tttaacagcg cgatgtcgtt tgggtggacc agggcgaaga aacggctcgc cggaaaggcg
      241 aggaggggag gcaccagccc gagctgctca gccagcctcg gactgacctg catcatatct
      301 tcggggatat gccattccca cagcgcgacg gcgcccgcct cgatggcgcg gccgagccgc
      361 tcggcatcgg cgagcagagg cagggagccg cgagcccctg aggcactgct gaaatcgatc
      421 atatgaagtg tgtgggggtg gtaagccgta ccggtaagcc tcttggacgc aacgcctggc
      481 cgcgctaggt tcgtggcagc aatcccctct taagtccggg cgtccatcct gtctttaaac
      541 taagacatat gcctgccgct tccgtgaagc ggctcgccga tcttcgtgtt cccatgtccc
      601 agttcgcgcc cttcacctat gacgtgccgt gcacgcaatg tccgttgcgg gtctgcgaag
      661 cattccgcac catcagcgag gacgagatcg aattcatcgc caagctgaaa ttgggcgagc
      721 tgcgcaccgg acccgccacg acgctgctcc gcgagggctc cgcctcggag cagctcttta
      781 ccctgctcga gggttgggcc ttccgctaca agacgttgga ggacggcaga cggcagatca
      841 cgagcttcgt tctgcccggc gatctcgtcg gcatccaatc ctcgatgcaa ggcgagatgg
      901 atcattcggt cgatacgctg accgcggcgg tcttttgcac ctttccccgc gagcgggtgt
      961 gggagttgta caaacgctgc cccagcctcg ccttcgacgt gacttggctc gcggccaccc
     1021 aggagatgat cctaaacgaa aacctgctga gcatcggccg ccgcaacgcc ttggaatgcg
     1081 cggcctacta cctcttgcat ctttatctcc gcgccgcgga tatcggcctc gtcaccgagg
     1141 gcaaggtccg attccccttc acccaggagc atcttgccga tgctttgggg ctctctcttg
     1201 tgcacaccaa caagacactc aggcggcttt ccgcgcgcgg cctgatgcaa tgggagcatg
     1261 gctggtgctt catccccgat gtcggcgctt tggctgaaat cgccaaattc gacttgaacc
     1321 agccccgcaa acgtcctttg ctctaagggc gaccacgacg atgcccccac tcgacgctcc
     1381 attttctgca cggctcgacg cctcagacct ctcagcccag tggcttccct tcaccgccaa
     1441 tcgcgccttc aggcggcgcc cgcggctgat cgatcgcgcc gagggcatgc attatttcgc
     1501 cgccgacggc cgcaacctgc tcgacgccat gtccgggctc tggtgctgca acgccggcca
     1561 ttgccgcgag ccgatcgtcg aggcgatccg ccgccaggcc ggatggctcg attatgctcc
     1621 ggccttccag gtcggccacg cgcccgcctt tcaactcgcc gcacgcctcg ccaagatggc
     1681 gcctggcgat ctcgaccacg tcctgttctg caactcgggc tccgaggcag tggacagcgc
     1741 gctcaagatc gcgctcgctt accataagct cgccggcgcc agcacgcgca cgcgcttcgt
     1801 ctcccgcgac cgcagctatc acggctcctg tttcggcggc acttcggtcg gcggcatcgc
     1861 ccccaaccgc aacctgttcg agccgctgct cgacgtcgat cagatccgct cgacctattc
     1921 ccgcgagcgc caagcatacg cgcgcggcga accggcctgg ggcgccgacc tcgccgacga
     1981 cctcacagcc attctcgcgc tcgaggagag ggcggccatc gccgccgtca tcgtcgagcc
     2041 gatggcgggg tcggcgggag tgttcgccag cccgcaaggc tatctgcagc gcttgcgcga
     2101 gatcacccgc gcgcatggca tcttgctgat cttcgacgag gtgatcacgg gcttcggccg
     2161 gctcggccac gcctttgccg ccgagcgcta tggcgtcgtc cccgacatga tctgcttcgc
     2221 caagggcgtg agcaacggcg cggcgccgct tggcggcgtg ctggtgcgcg gccgcatcca
     2281 tgacgccttc atgcagggcc cggagcatgt gcccgagctt gcccatggct tcacctattc
     2341 gggccacccg ctcggcgttg ccgccgcttc ggcggctctc gacctttatc gcgaggaggg
     2401 cttgttcgag cgcgccgtcg cgctcgaacc cgtcttcgcc gatgccgtgc atgcgttgaa
     2461 gggagcgccc cacatcgtcg acatccgcag catcgggctc gccgccggca tcgacctcgc
     2521 cccgatcgac ggttcgccgg gtttgcgcgg cgcccgcgcc ttcgaggccg cctttttcga
     2581 ggaggatctt gtgctccgcg ccgtgggcga tacgctggta ttggccccgg cgctgatcgc
     2641 caccgaagcg gagatcggcg ccatcaccga aaagctcagg cgcatcctcg cggcgctgcg
     2701 ttgagccgcg caccgcttgg tttcgccggt ctaggctgct atcaagaagc ctcggcagcc
     2761 ccgccagatt actgttgcct tgcaggggcc tgtcgccgca tgattttcgc ctagggggaa
     2821 tgcggaattg gcggacacta ttgccatggc ctctaggcga agcgagtcct cgggaggact
     2881 ggccatgctc gacgtgatcg tgttgatcgc catggcggtg acggccttgg ccttcgccgt
     2941 cgggctgatc gtccaatccg gcatcgccca gatccccgcg ctgattgccg ccgccgcgct
     3001 ctatctggtg atggcggcgt cctatttgat gctcgcgcgc ggcgcccgcc ccaccgcgtc
     3061 caccgaccgg ttgagcgagg tcgaggcggc gctcgagatc atcgacaagg acctgcagcg
     3121 catcgatcgc gtcgaggacg acgtctcccg gctcgatctg cttgccgacc gcgtcgagcg
     3181 tctcgaccag gcgctcagcg aatatgccgc gaccgcgccg atcggcggcg tcggccgctc
     3241 cgatcaagtc agcaacgaat tcgacgaggt gcatgccaag atcgacgccg tgcgcgccga
     3301 tctcgagggc gaggc
//
LOCUS       DATNAE010000018         2593 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_4229527, whole genome shotgun sequence.
ACCESSION   DATNAE010000018 DATNAE010000000
VERSION     DATNAE010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4229527"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2593
                     /locus_tag="VN518_00330"
     CDS             <1..>2593
                     /locus_tag="VN518_00330"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086704.1"
                     /note="Catalyzes the rate-limiting step in dNTP synthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HXE03050.1"
                     /translation="INWKVIEEQKVAALVTGSKILSERLNAILKACVNCEADGDGCFD
                     PKCNPALKREVKAARKAKVPDNAIRRVIQFARQGYTSIHVPVYDTDWDSEAYRTVSGQ
                     NSNNSVRVTDEFLRAVEADENWNLTFRKDGKIAKTVKARELWEEIGSAAWACADPGIQ
                     FHTTINDWHTCPASGPIRASNPCSEYMFLDDTACNLASLNLMAFRSEDGSFDVEAYEH
                     AIRLWTVVLEIAVTMAQFPSRRIAQLSYEYRTLGLGYANIGGLLMASGIPYDSPEARS
                     LCGALSAIMTGISYATSADMARELGPFPGFEQNREHMLRVMRNHARAARGEAEGYEDL
                     KTAPVPLDHASCPDPKLLARAVHAWERAIELGRQHGYRNAQATVIAPTGTIGLVMDCD
                     TTGIEPDFALVKFKKLAGGGYFKIINRMVPEALAALGYDEAAVKRIIDYAVGRGSLAG
                     APGINYETLSARGFTAEKITAAEKAVATAFDIKFAFNKWTLGEVFVTGPLKLSAERID
                     QPDFDLLTEIGFSKREIEAANEYACGAMTLEGAPGLDPKHLPVFDCASPCGRKGKRFL
                     SVESHIRMMAAAQPFVSGAISKTINMPNEASVEDCKEAYLLSWRLALKANALYRDGSK
                     LSQPLNAQLLADEEDEQEEVLASITADQPQAQRAMVLAERLTQRVAELEEEKARLERH
                     LREDRRGLREKLPDRRTGYIQKAVVGGHKVYLHTGEYKDGRLGEIFLDMHKEGAAFRS
                     LMNNFAIAVSVGLQYGVPLEEFVEAFTFTRFEPAGLVRGNAAIKNATSILDYIFRELA
                     ISYLDRKELAHVNPNENGHTAVGSGEDQKVREAAEFETETRVSRGFARKRAGDQDKLQ
                     LASLNRVP"
BASE COUNT          505 a          858 c          802 g          428 t
ORIGIN      
        1 catcaattgg aaggtgatcg aggagcagaa ggtcgcagca ctcgtcaccg gctccaagat
       61 tctctccgag cggctcaacg ccatcctcaa ggcttgcgtc aattgcgagg ccgacggcga
      121 cggctgcttc gatccgaaat gcaacccggc gctgaagcgc gaggtgaaag ccgcgcgcaa
      181 ggccaaggtg ccggacaacg ccatccgccg ggtgatccaa ttcgcccgtc agggttacac
      241 ttcgatccat gtcccggtct acgacaccga ttgggactcc gaggcctacc gcaccgtctc
      301 gggccagaac tccaacaact cggtgcgcgt caccgatgag ttcctgcgcg cggtcgaggc
      361 cgacgagaat tggaacctca ccttccgcaa ggacggcaag atcgccaaga cggtgaaagc
      421 ccgcgagctg tgggaagaga tcggcagcgc cgcctgggct tgcgccgatc caggcatcca
      481 gttccacacc accatcaacg actggcacac ctgcccggcc tcaggcccga tcagggcctc
      541 caatccttgc tcggaataca tgttcctcga cgacaccgcc tgcaatctcg cctcgctcaa
      601 tctgatggcg ttcagaagcg aggacggtag cttcgacgtc gaggcttacg agcacgccat
      661 caggctgtgg acggtggtgc tcgagatcgc ggtgaccatg gcgcagttcc cctcgcgccg
      721 cattgcgcaa ctctcctacg aataccggac gctcggcctc ggctacgcca atatcggcgg
      781 cctgttgatg gcgtcgggca ttccttacga ctctccggaa gcgcgttcgc tgtgcggggc
      841 gctctccgcc atcatgaccg gcatctccta cgccaccagc gccgacatgg cgcgggagct
      901 tgggccgttc ccgggcttcg agcagaaccg cgagcacatg ctcagggtca tgcgcaatca
      961 cgcccgcgcc gcgaggggtg aggctgaggg ctacgaggat ctcaagaccg cgccggtgcc
     1021 actcgaccac gcctcctgcc ctgatccgaa gctgctcgcc cgcgccgtgc atgcgtggga
     1081 gcgcgccatc gagcttggcc ggcagcatgg ctaccgcaac gcgcaagcga cagtgatcgc
     1141 gccgaccgga accatcggtc tcgtcatgga ctgcgatacc accggcatcg agccggactt
     1201 cgcgctggtc aaattcaaga agctcgccgg cggcggctat ttcaagatca tcaaccgcat
     1261 ggtgcctgag gccctcgccg cgctcggcta tgacgaggca gcggtcaagc gcatcatcga
     1321 ctatgccgtc ggccgcggct cgctcgccgg cgctcccggc atcaattacg agacgctgtc
     1381 agcgcgcggc ttcaccgccg aaaagatcac ggcggcggaa aaggcggtgg cgaccgcctt
     1441 cgacatcaaa ttcgccttca acaaatggac gcttggcgag gtcttcgtca ccgggccgct
     1501 caagctgtcg gctgagcgca tcgaccagcc agacttcgac ctcctgaccg agatcggctt
     1561 ctccaagcgg gagatcgaag ccgccaacga atatgcctgc ggagccatga cgctcgaagg
     1621 cgcgccgggg ctcgatccca agcatctgcc ggtgttcgac tgcgcctcgc cctgcggccg
     1681 caagggcaag cggttcctgt cggtggaaag ccatatccgc atgatggcgg cggcccagcc
     1741 cttcgtgtca ggggcgatct ccaagaccat caacatgcca aacgaggcga gcgtcgagga
     1801 ttgcaaggag gcctatctgc tctcctggcg gctcgcgctc aaggccaacg cgctttatcg
     1861 cgacggctcc aagctgtccc agccgctcaa cgcccagctt ctggccgacg aggaggacga
     1921 gcaggaggag gttctggctt ccatcaccgc cgaccagccg caggcccagc gcgccatggt
     1981 cttggccgag cgtcttacgc agcgcgtggc cgagctcgag gaagagaagg caaggctcga
     2041 gcgtcacttg cgcgaagacc gtcgtggtct gcgggagaaa ctgccggata ggcgcaccgg
     2101 ctatatccag aaggccgtgg tcggcgggca taaggtctat ctgcataccg gcgagtacaa
     2161 agacgggcgc ttgggcgaaa tcttcctcga catgcacaag gagggcgcgg ccttccggtc
     2221 gctgatgaac aatttcgcca tcgccgtgtc ggtgggcctg caatatggcg tgccgctcga
     2281 ggagttcgtc gaggccttca ccttcacccg cttcgagcct gccggcctcg tgcgcggcaa
     2341 cgcggcgatc aagaacgcca cctcgatcct cgactacatc ttccgggagc tcgcgatctc
     2401 ttatttggat cggaaagagc tcgcgcacgt caatccaaac gagaacggcc ataccgcggt
     2461 cggctcgggc gaagaccaga aggtacgcga agctgccgag tttgagaccg agaccagggt
     2521 gtcgcgcggc ttcgcgcgca agcgggccgg agaccaggac aagcttcaat tagcgagcct
     2581 gaaccgtgtt ccg
//
LOCUS       DATNAE010000019         2406 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3332387, whole genome shotgun sequence.
ACCESSION   DATNAE010000019 DATNAE010000000
VERSION     DATNAE010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3332387"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..1321)
                     /locus_tag="VN518_00335"
     CDS             complement(62..1321)
                     /locus_tag="VN518_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HXE03051.1"
                     /translation="MTPVELLKSMFQAAVDAALPSICVPAHLPPRPKGRTVIIGAGKA
                     SGAMASALEGAWEGPLEGLVVTRYGYRVPTERLEVVEAAHPVPDAAGRDAAKRIFELV
                     QGLSKDDLVLCLISGGGSALLALPAEGVSLEDKQAVNKALLKSGATISEMNAVRKHLS
                     AIKGGRLAAAASPAKVVALMISDVPGDDPSIIASGPTVPDPSTNKDALAIIEKYKIDV
                     PASVLKRLKTADETPKPGDPRFAGVENIMIATPQASLEAAAAVARKAGVKPVILGDSI
                     EGESRDVALVHAGIARQCAMRGQPDVPPCVLISGGETTITLKGKGKGGRNTEFLLALA
                     IALHGMKGIYGLAGDTDGIDGSEDNAGAIITPDTLARAEKAGLNAKAMLADNDPWTFF
                     KGIGDLLMTGPTLTNVNDFRAVLIEKD"
     gene            1441..>2406
                     /locus_tag="VN518_00340"
     CDS             1441..>2406
                     /locus_tag="VN518_00340"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016921633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HXE03052.1"
                     /translation="MSVQPRESAEKPGTRTTDQHQKPKSDIEISQAAKMRPIVEVAAE
                     KLDIPPEFVLPYGHYKAKISLDYIASLANRTDGKLILVTAITPTPAGEGKTTTTVGLG
                     DALNHIGKKAVVCVREPSLGPCFGVKGGAAGGGYAQVVPMEDINLHFTGDFHAIGAAN
                     NLLAALVDNHVYWGNKLDIDTRRVTWRRAVDMNDRALREIVTALGGAANGYPREAGFD
                     ITVASEVMAIFCLASDLADLQRRLGQIQVGQTRDKKPVTARDLSASGSMAALLKDALA
                     PNLVQTLENNPAFIHGGPFANIAHGCNSVIATQTGLKLADYVVTEA"
BASE COUNT          427 a          778 c          770 g          431 t
ORIGIN      
        1 acggtggtct gcgagaccga ttcggagatc tagccgttgc cggcggcagc gatcggtctg
       61 actagtcctt ctcgatcagc acggcgcgga aatcgttgac gttggtcagc gtcggtccgg
      121 tcatcagcaa atcgccgatc cccttgaaga acgtccaggg atcgttgtcg gcgagcatgg
      181 ccttggcgtt gaggcctgct ttctcggcgc gggcaagcgt gtcaggtgtg atgatggcgc
      241 cggcattatc ctcagagccg tcgatgccgt cagtgtcgcc ggcaaggccg tagatgccct
      301 tcatgccgtg gagcgcgatg gcgagcgcga gcagaaactc ggtgttgcgc ccgcctttgc
      361 ctttgccctt cagcgtgatc gtggtctcgc cgcctgagat cagcacgcag ggcggaacgt
      421 caggctggcc gcgcatggcg cattgccggg caatgccggc gtggaccaag gccacgtcgc
      481 gggattcgcc ttcgatcgag tcgccgagga tcacgggctt gacgcccgct ttccgcgcaa
      541 ccgcggctgc cgcttcgagc gaggcctgag gcgtggcgat catgatgttc tcgacgcccg
      601 caaaccgggg atcgcctggc ttcggcgtct cgtcggcggt cttaaggcgc ttgagcacgc
      661 tcgccggcac atcgatcttg tatttctcga tgatggcgag ggcgtccttg ttggtggagg
      721 ggtcgggaac cgtcgggccg gaggcgatga tagaaggatc gtcgccagga acgtctgaga
      781 tcatcagcgc cacaaccttc gccggcgatg cggctgcagc gagcctgccg cccttgatcg
      841 ccgagaggtg tttgcgcacg gcgttcatct cgctgatggt ggcgccagac ttgagcagtg
      901 ccttattcac cgcctgcttg tcttcgaggc tcacgccttc cgccggcaaa gcgagcagcg
      961 ccgagccgcc gccggagata agacagagaa cgaggtcgtc cttgctcagc ccctgcacca
     1021 gctcgaagat gcgctttgcc gcgtcgcggc ccgcggcgtc gggaaccggg tgggcggcct
     1081 cgacaacctc caggcgttcg gtcggcacgc ggtagccgta gcgggtcacg accagtcctt
     1141 cgagcggtcc ctcccaggcg ccctcgagcg cagatgccat ggcgcctgag gctttgccgg
     1201 cgccgatgat gacagtgcgt cccttgggcc tcggcgggag atgtgccggc acgcagatcg
     1261 aagggagggc tgcgtccacc gctgcctgaa acatggattt cagaagctcg accggcgtca
     1321 ttcaaggcct ccaggtgggc gctcacctcg cacagacgat tgcaagaagg caagcgaatg
     1381 atttaggttg cctattcctg ttgcaaggcg ttgtgccgta acccctgggg cagttgattt
     1441 atgtcagttc agccgcgtga aagtgccgag aaaccaggca cgcgcacaac cgatcagcac
     1501 caaaagccaa aatccgacat cgaaatctcg caagccgcca aaatgcggcc gatcgtcgag
     1561 gtggcggcgg agaagctcga cattccgccg gaattcgtcc tgccttacgg ccactacaag
     1621 gcgaagatct cgctcgacta catcgcttct ctcgccaatc gcaccgacgg caagctgatc
     1681 ctcgtcaccg ccatcacgcc cacgcctgcc ggcgaaggca agaccaccac caccgtcggg
     1741 ctcggcgatg cgctgaacca tatcggcaag aaggcggtcg tctgcgtgcg cgagccgagc
     1801 ctcggcccct gtttcggcgt gaaggggggt gccgccggcg gcggctatgc ccaggtcgtg
     1861 cccatggagg acatcaatct ccatttcacc ggcgacttcc acgccatcgg cgccgccaac
     1921 aatctgctcg cggcgctggt cgacaatcac gtctattggg gcaacaagct cgacatcgac
     1981 acccgccggg tcacgtggcg ccgcgccgtc gacatgaacg atagggcgct gcgcgaaatc
     2041 gtcaccgcgc ttgggggggc cgccaacggc tacccgcgcg aagcaggctt cgacatcacc
     2101 gtggcgagcg aggtgatggc catcttctgc ttggcttccg acctcgccga tctgcaacgc
     2161 cggctcgggc agattcaggt cggccagacg cgggacaaga aacccgtcac ggcgcgcgac
     2221 ctctcagctt ccggctcaat ggcggcgctg ctcaaggacg cgctcgcgcc taacctggtg
     2281 cagacgctgg agaacaaccc agccttcatt catggcggtc ccttcgccaa tatcgcccat
     2341 ggctgcaatt cggtgatcgc gacccagacc ggcctcaagc tcgccgacta tgtcgtgacc
     2401 gaggcc
//
LOCUS       DATNAE010000020         2010 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_923096, whole genome shotgun sequence.
ACCESSION   DATNAE010000020 DATNAE010000000
VERSION     DATNAE010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923096"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1271
                     /locus_tag="VN518_00345"
     CDS             <1..1271
                     /locus_tag="VN518_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HXE03053.1"
                     /translation="NGATARVAARDKGTIIVVEGYVDAIRMAAAGFENTVAPLGTALT
                     ERQLELLWAFADEPILCFDGDKAGQKAAWRAADLALPLLKPGKSLRFALLPEGQDPDD
                     LARSGGRGAVEEVISAARPLAEMIWSREIEGGNFATPERRAALEARINELSNSIRDEV
                     VRRYYRQDLIERLGRTFAPEPVRNFPGRGAFSESPRRFQPRAGGQPRAGGKFSPGRPA
                     GLAGLSALRRGPYQAASPQLANSPIMRGQRSAISRREALILQCLVNYPWLLHDHLEEV
                     AALELAHGEAHKLRAGIIAAFANEHHHSPDPSEQAEKMRADLEKAGFSQFLQRVDRAI
                     TTGAVWGARSGAAREDVLSTWHQLVVLHRKTHALLREKKDAELALGEEPSEANMAWLK
                     DVSHRLESLEGTEAQIEGFGELSGRFQKSV"
     gene            1522..>2010
                     /locus_tag="VN518_00350"
     CDS             1522..>2010
                     /locus_tag="VN518_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
                     /protein_id="HXE03054.1"
                     /translation="MATKAKTLQVKDKEKDDKAADAPEKDAPDAPSPLLDLSDAAVKK
                     MIKQAKKRGFVTFDQLNEVLPSDQTSPEQIEDIMSMLSDMGINVTEADDSEGEEDKDE
                     AGDDDTDNELVEVTQKAVTEVKKSEPGERTDDPVRMYLREMGTVELLSREGEIAIAKR
                     IEA"
BASE COUNT          407 a          646 c          661 g          296 t
ORIGIN      
        1 acaacggcgc cacggcgcgc gtggccgccc gcgacaaggg cacgatcatc gtcgtcgaag
       61 gctacgtcga cgccatccgc atggccgccg ccggcttcga gaacacggtc gcgccgctcg
      121 gcaccgcgct caccgagcgc cagctcgagc tgctgtgggc cttcgccgac gagccgatcc
      181 tctgtttcga cggcgacaag gcaggacaaa aggcggcgtg gcgcgccgcc gatctcgcgc
      241 tgccgctgct caagcccggc aagagcctgc gcttcgcgct gctgccggaa ggccaggacc
      301 ccgacgatct cgcgcgctcg ggcgggcgcg gcgcggtcga ggaagtgatc tcggcggcgc
      361 gtccgctcgc cgagatgatc tggtcgcgcg agatcgaggg cggcaatttc gcgactcccg
      421 aacgccgcgc cgcgctggaa gcccgcatca acgagctttc caattccatc cgcgacgagg
      481 tcgtgcgccg ctattatcgc caggatctga tcgagcgtct ggggcgcacg ttcgcgccgg
      541 agcccgtgcg aaacttcccc ggccgcggcg ccttcagcga atcaccccgc cgtttccagc
      601 cccgcgccgg tggccagccg cgcgcgggcg gaaaatttag ccccggacgg ccggcgggcc
      661 tcgccggctt aagcgcgctc cgacggggtc cctaccaagc cgcgagcccg cagcttgcca
      721 acagcccgat catgcgcggc cagcgctcgg cgatttcccg ccgcgaggcg ctgatcctgc
      781 aatgtctggt taactacccc tggttgctgc acgaccatct ggaggaggtg gcggccctgg
      841 aacttgccca tggcgaggcc cacaagctcc gcgccggcat catcgcggcc ttcgccaacg
      901 agcatcatca ttccccggat ccctccgaac aggccgagaa aatgcgggcc gaccttgaga
      961 aggccggatt ttcgcaattc cttcaaaggg ttgatcgggc gatcacgacg ggcgcggtgt
     1021 ggggcgcaag aagtggcgcg gcgcgagagg atgttctttc cacctggcat caactcgttg
     1081 tcttgcatcg gaaaactcac gccctactta gggagaagaa agacgcggag ctggccctcg
     1141 gcgaggaacc cagcgaggcc aacatggcct ggctgaagga tgtgagccac cggctggagt
     1201 cgctggaggg caccgaggcg cagatcgagg ggtttggcga gctttccggg cggttccaga
     1261 aaagcgtgtg accagaagaa tcatgcggac ggagccggac gaaaccgcaa aaggactcgc
     1321 caaacccatg gtttggctgc aaaaacaggg ttaagcgagg cttaatacga ctccggtatc
     1381 ggaagtcacg aaccttatcg aaagagattc ggggggtggc gatcttttgc gccgcccttg
     1441 ttgcgtccta acagccgcaa caaagaaaca gcgggcgcgg caacgcgcct ctcgtgaagc
     1501 gcgcgttttg gagagcactg aatggccacg aaggcaaaga cgctgcaggt caaggacaag
     1561 gaaaaagacg acaaggcagc ggatgctccc gaaaaggacg cccctgatgc gccgtcgccg
     1621 ttgctcgacc tctcggatgc cgccgtcaag aagatgatca agcaggccaa gaagcgcggc
     1681 ttcgtgacct tcgatcagct caacgaagtg ctgccctccg accagacctc gcccgagcag
     1741 atcgaggaca tcatgtcgat gctctcggac atgggcatca atgtcaccga ggctgacgat
     1801 agcgagggcg aggaggacaa ggacgaggcc ggcgacgacg ataccgacaa cgagctcgtc
     1861 gaggtcacgc agaaggccgt caccgaggtc aagaagagcg agcccggcga gcgcaccgac
     1921 gatcccgtgc gcatgtatct gcgcgagatg ggcaccgtcg agctgctgtc gcgcgagggt
     1981 gaaatcgcga tcgccaagcg catcgaggcc
//
LOCUS       DATNAE010000021         2572 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_1222931, whole genome shotgun sequence.
ACCESSION   DATNAE010000021 DATNAE010000000
VERSION     DATNAE010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1222931"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="VN518_00355"
     CDS             <1..385
                     /locus_tag="VN518_00355"
                     /inference="COORDINATES: protein motif:HMM:NF017135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050080 - malonyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="malonyl-CoA decarboxylase family protein"
                     /protein_id="HXE03055.1"
                     /translation="LHAQPGRGGKGGLRELLAAPEWHEDEAAMQVLREPLLRLCARYL
                     MLEKTPAGRPRDAVAHFHLSNGARLERLDWAADLSPKGVQQSAGIMINYLYRLSEIED
                     NHETYTDEGRIAASPQIRSLLRRDT"
     gene            510..764
                     /locus_tag="VN518_00360"
     CDS             510..764
                     /locus_tag="VN518_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03056.1"
                     /translation="MSLMDAFYRQDKTPEIKPGDCYSRAHGLGLKETATVLDLRNDPL
                     GIPHVRYCVRFERQTSEHSETNLRMLALGAFRSVYRERLV"
     gene            920..1004
                     /locus_tag="VN518_00365"
     tRNA            920..1004
                     /locus_tag="VN518_00365"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:954..956,aa:Leu,seq:tag)
     gene            1070..2458
                     /gene="tig"
                     /locus_tag="VN518_00370"
     CDS             1070..2458
                     /gene="tig"
                     /locus_tag="VN518_00370"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HXE03057.1"
                     /translation="MQITETNAEGLKREFKVTVASSDIEQKMLDRLGEIGRTIRLPGF
                     RPGKVPMPVLRKRYGASVMGEVLEKAVSDSSAEAMRERNLRPALQPKIEIVSFKEGTD
                     LEYKMAIELLPEIKPVDFSQLELERLRPEIPEAEIDKAVERIAEPNRKSEPVDRPAAS
                     GDIAVIDFVGKVDGKEFSGGSAQGHSLELGSGRFIPGFEDQLIGAKAGEHHDVKVTFP
                     AEYGSEELAGKDAEFAVDVKEVRAKQALALDDDMAKEMGFDALDGLRKAVREQLERDY
                     GGLARQRLKRALLDQLAAKHDFPVPPGMVDLEFDTIWKQFQEAREQNKGELDEDAGKS
                     DEELKTEYRSIAERRVRLGLLLSEVGRANAITVNQDEINRALGEEARRHRGYEKQVVE
                     FYRNNPEALANLRAPIFEEKVVDFITEMANVAEKTVSIEDLLKADSEDAAEPTADAAA
                     DKAKRKPRKKAE"
BASE COUNT          532 a          802 c          847 g          391 t
ORIGIN      
        1 gctccacgcc cagccggggc gggggggcaa aggcggcctg cgcgaattgc tcgcggcacc
       61 ggagtggcat gaggacgagg cggccatgca ggtgctgcgc gagcccctgc tgcgcttatg
      121 cgcacgctac ctcatgctgg aaaagacccc ggcgggccgg ccgcgcgatg ccgtcgcgca
      181 tttccatctc agcaacggcg cccggctgga acgtctcgac tgggccgcag atctctcgcc
      241 caagggggtg cagcaatcgg ccggcatcat gatcaactat ctctatcggc tgtcggagat
      301 cgaggacaac cacgagacct ataccgatga ggggcggatt gccgcctcgc cgcaaatccg
      361 tagcctcctg cggcgggata cttagcccgc attttgctaa aatactcacc tgaaacaaaa
      421 gcttatgcga agcttatgcg ttaagggttc gttcagattt taacgtttat cctgcccata
      481 ttcggaaccc actgaccggg caccagcaaa tgtccctcat ggacgccttt taccgccagg
      541 acaagacgcc ggaaatcaag ccgggcgatt gctacagccg cgcgcatggt ctcgggctga
      601 aggaaaccgc aaccgtgctc gatctgcgca acgatccgct cggcatcccc catgtccgct
      661 attgcgtgcg gttcgaacgc cagacctccg aacatagcga gaccaatctc cgcatgctcg
      721 ccctcggcgc cttccgcagc gtctaccgcg agcgcctcgt ctagtaccga ggcccgcggc
      781 cgggtatcac cggggcggcg agaccggcat tgcgccggcg tttggatagt ttcactcccc
      841 aaccctctgt gctatgaagc gcggcccgcc gggcgaggcg gggccgtgcg gttcggcgca
      901 agccgggtcc gagcgccggg cgggcgtggc ggaattggca gacgcactgg atttaggttc
      961 cagcgacctc ggtcgtgggg gttcaaatcc ccccgcccgc accacgcccg acagagatca
     1021 acagcgcggc tgcggaacga cgcagccctt caacggacac ccgagcaaga tgcagatcac
     1081 cgagaccaac gcagaaggcc tgaagcgcga gttcaaggtc accgtcgcct cgagcgatat
     1141 cgagcaaaag atgctggacc ggctcggcga gatcggccgc accatccggt tgcccggctt
     1201 ccggcccggc aaggtaccga tgccggtgct gcgcaagcgc tatggcgcct cggtgatggg
     1261 cgaggtcctc gagaaggcgg tcagcgacag ctcggccgag gcgatgcgcg agcgcaatct
     1321 gcgcccggcg ctgcagccca agatcgagat cgtctcgttc aaggaaggca ccgatctcga
     1381 atacaagatg gcgatcgagc tgctgcccga gatcaagcct gtcgatttca gccagctgga
     1441 gctcgagcgg ctgcgcccgg agattcccga ggcggagatc gacaaggcgg tggagcgcat
     1501 cgccgagccc aaccgcaaga gcgagccggt cgatcggccg gcggcgagcg gcgacatcgc
     1561 cgtcatcgac tttgtcggca aggtcgacgg caaggagttc tccggcggca gcgcccaggg
     1621 ccattcgctc gagctcggct cgggccgctt catccccggc ttcgaggatc agctcatcgg
     1681 cgccaaggcc ggcgagcacc acgatgtcaa ggtgaccttt ccggccgaat acggcagcga
     1741 ggagctcgcg ggcaaggacg cggaattcgc cgtcgacgtc aaggaagtgc gcgcgaaaca
     1801 ggcgctcgcc ctcgacgacg atatggcgaa ggagatgggc ttcgacgcac tcgacgggct
     1861 gcgcaaggcg gtgcgcgagc aattggagcg cgactatggc gggctggccc gccaacgcct
     1921 gaagcgcgcg ctgctcgatc agctcgcggc gaagcacgat tttcccgtgc cgccgggcat
     1981 ggtggatctt gaattcgaca ccatatggaa gcaattccag gaggcgcgcg agcagaacaa
     2041 gggcgaattg gacgaggatg ccggcaagag cgacgaggaa ttgaagaccg agtatcgcag
     2101 catcgccgaa cggcgcgtgc gactcggctt gctgctatcg gaggtcgggc gcgccaacgc
     2161 cattaccgtc aaccaggacg agatcaatcg cgcgctcggc gaggaggcgc ggcgtcatcg
     2221 cggctacgaa aagcaggtcg tcgagttcta ccgcaacaac cccgaggcgc tggccaatct
     2281 gcgcgcgccg atctttgaag aaaaggtggt cgacttcatc acggaaatgg cgaacgtcgc
     2341 cgaaaagacg gtgtcgatcg aggacctgct gaaggcagat tcggaagacg cggccgagcc
     2401 gacggcggat gcggcggccg acaaagccaa acgcaagccg cgcaagaagg cggaatgagg
     2461 aacgcaaaag gtttattggg cgttatcggc gtcaggagcc atcatgagtg agccattcga
     2521 gatccaaatg aacacgctgg tgccgatggt ggtcgagcag accaaccgtg gc
//
LOCUS       DATNAE010000022         2505 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3932859, whole genome shotgun sequence.
ACCESSION   DATNAE010000022 DATNAE010000000
VERSION     DATNAE010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3932859"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="VN518_00375"
     CDS             <1..317
                     /locus_tag="VN518_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXE03058.1"
                     /translation="GKYEGGALPEGSRKRLFDRLGRYEMGNGPRAISAYVALARKHGL
                     DPAQMALAFTASRPFVTSTIIGATTLTQLKTDIEGCLLRLSDAVIQDIEQLHLTYPNP
                     CP"
     gene            340..876
                     /gene="thpR"
                     /locus_tag="VN518_00380"
     CDS             340..876
                     /gene="thpR"
                     /locus_tag="VN518_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HXE03059.1"
                     /translation="MPRLFSGIEIPAAVARRLTLVRAGLNGARWIDPENYHVTLRFLG
                     DVDGTTARDFAHGLGDIAAPPFELRLDMLGSFGGRKPRAIFASIAPSNALESLQRAHE
                     RAARAAGLPPEPRNYTPHVTVARLRGTSSESVATYLERQGGFSSEPFEVTRFVLFSSR
                     ASVGGGPYVVEAAYPLAV"
     gene            complement(873..1370)
                     /locus_tag="VN518_00385"
     CDS             complement(873..1370)
                     /locus_tag="VN518_00385"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HXE03060.1"
                     /translation="MVTRILFVCLGNICRSPMAEGVFRRVAEEEGLIDRFEIDSAGLG
                     DWHIGQAPDHRAQKAARSRGVDISSQSARQVVYEDFERFDLVLAMDGSNLAELKARAP
                     HEARGKIRPFLDFAPQVGTKDVPDPFFGGAEGFDHVLDLIEAASRGLLASLTGKEKVR
                     AKRGA"
     gene            1519..1920
                     /locus_tag="VN518_00390"
     CDS             1519..1920
                     /locus_tag="VN518_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008933658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HXE03061.1"
                     /translation="MMAFDDASDLRNAALGREISLRKFAEWEKRARTDFWSKLRRFAG
                     QVPFVEDIVAGYYCALDPATPMRVRGMLLAAIAYFILPFDFLPDVIAGLGFADDAALF
                     TAVLALVSAHITPTHRAAAARALDKELPKTT"
     gene            complement(1933..>2505)
                     /locus_tag="VN518_00395"
     CDS             complement(1933..>2505)
                     /locus_tag="VN518_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006937847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HXE03062.1"
                     /translation="LAPGETIAVLGAAGGAGLAAVEIASLMGGRVIAVASSQDKLAIA
                     KARGAAEGIDYQTEDLKIRLKELTGGTGVDVLYDCVGGPHAEPALRAMAWEGRYLVVG
                     FAAGEIPRFPLNIVMLKGCAVVGVLLSGFVARHPDRHRANLVELLEWCREGKIMPHIH
                     GVFPLEETAKALGLIEGRKVTGKVIVNPQL"
BASE COUNT          426 a          872 c          755 g          452 t
ORIGIN      
        1 ccggcaaata tgaggggggc gcgcttcctg aaggctcacg caaaaggttg ttcgaccgtc
       61 tgggccgcta cgagatgggc aacggccccc gcgccatctc ggcctatgtt gcgctcgcgc
      121 gaaagcatgg cctcgaccct gcgcaaatgg cgcttgcctt caccgcttcg cggcctttcg
      181 tcacgtcaac catcattggc gcgacgactc tcacccagtt aaaaaccgat attgagggtt
      241 gcctgctcag attgtccgac gcagtaatcc aagatatcga gcaacttcac ctcacctatc
      301 ccaatccctg cccttaagcc ttcgccgagg aaccgcggaa tgccaaggct attttccgga
      361 atcgagatac cagctgccgt ggcgcgccgg ctgaccctcg tgcgggcggg cctcaacggc
      421 gcgcgctgga tcgatcccga gaactaccat gtgacgctgc gcttcctcgg cgacgtcgac
      481 ggcaccacgg cgcgcgactt cgcgcacggg cttggagaca tcgccgcccc acccttcgag
      541 ctcaggctcg atatgctcgg cagttttggc ggcaggaagc cccgggcgat cttcgccagc
      601 atcgccccga gcaacgcgct tgagtccttg cagcgggcgc atgagcgcgc cgcgcgcgcg
      661 gccggcctgc cgccggaacc gcgcaattat acgccccacg tcaccgtggc gcgcttgaga
      721 ggcacgagct ctgaatccgt cgccacctat ctcgagcgcc agggcgggtt ctcgagcgag
      781 cccttcgagg tgacccgctt cgtgctgttc tcctcgcgcg cttccgtcgg cggcggcccc
      841 tatgtggttg aagccgccta cccgctcgcc gtctaggcgc cgcgtttcgc ccgcaccttt
      901 tccttgccgg tgagcgaggc gagcagaccc cgcgaggcag cctcgatcag gtcgagcacg
      961 tgatcgaagc cctcggcgcc gccgaagaag ggatcgggca catcctttgt cccgacctgc
     1021 ggcgcgaaat cgaggaaggg acggatcttg ccgcgcgctt catgtggcgc gcgggccttg
     1081 agctcggcaa ggttggagcc gtccatggcg agcacaaggt cgaaccgctc gaaatcctca
     1141 tacaccactt gccttgccga ctggctggag atgtcaacgc cacgggaccg cgccgccttc
     1201 tgggcgcgat gatccggggc ctggccgata tgccaatcgc caagccctgc cgaatcgatc
     1261 tcgaagcggt cgatcagccc ctcctcctcg gccacgcggc ggaacacgcc ttcggccatt
     1321 ggcgaacggc agatattgcc gaggcagaca aagaggatgc gggtgaccat ggccggctgc
     1381 ttcgcatgag gacgccgccg tgacaaggcg cgaatggccc catccggcgc ccatgactct
     1441 ggcttcttgc gaaggcgcca cgcccatctt atgtcattgg ggacgtttgg cttcttcgta
     1501 cctggcgagg atacgaccat gatggctttt gacgatgcga gcgacctccg caatgcggct
     1561 ctcggcaggg agatttcctt gcgcaaattc gccgagtggg aaaagcgtgc gcgcaccgat
     1621 ttctggtcga agctgaggcg cttcgcgggc caagtgccgt tcgtcgagga catcgtcgcc
     1681 ggctattact gcgcgctcga cccggccacg ccgatgcgcg tccgcggcat gctgctcgcg
     1741 gccatcgcct atttcatcct gcccttcgat ttccttcccg acgtcatcgc cgggcttggc
     1801 tttgccgacg acgccgctct gttcacggcg gtactcgccc tcgtctcggc gcatatcacg
     1861 ccgacccacc gtgccgccgc cgcccgcgcc ctcgacaaag agctgccgaa gaccacgtaa
     1921 ggacctgaag cgctacaact gagggttgac gatcaccttg ccggtgacct tgcgcccctc
     1981 aatcaggccg agcgccttcg ccgtctcctc aagcgggaag acgccgtgga tatgcggcat
     2041 gatcttgcct tcccggcacc attcgagcag ctcgaccaaa ttcgcccggt gccggtccgg
     2101 atggcgcgcg acgaatccgc tcagcaacac gccgaccacc gcgcagcctt tgagcatcac
     2161 gatgttgagc ggaaagcgtg gaatttcgcc ggcggcaaag ccgaccacga gatagcgtcc
     2221 ctcccacgcc atggcccgta atgctggctc ggcgtggggg ccaccgacgc aatcgtagag
     2281 cacatcgacg ccggttcctc ccgtcagctc tttgagcctg atcttcagat cttccgtctg
     2341 gtagtcgatg ccctcggctg caccacgcgc cttggcaatg gcaagcttgt cttgtgatga
     2401 ggcgacagcg atgacgcgcc cgcccatgag gctcgcgatc tcgaccgcag ctagccccgc
     2461 gccgcccgcc gcgccaagca ccgcgatggt ttcgccgggc gcgag
//
LOCUS       DATNAE010000023         6745 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3333222, whole genome shotgun sequence.
ACCESSION   DATNAE010000023 DATNAE010000000
VERSION     DATNAE010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6745
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3333222"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="VN518_00400"
     CDS             complement(<1..381)
                     /locus_tag="VN518_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HXE03063.1"
                     /translation="MGVTLRRGTRLVIASHNEGKVREIADLLAPFGVEAVFAGKLGLP
                     ETEETGASFAENARLKAMAGARASGLPAFADDSGLEVKALGGEPGIHSARWGGAARDF
                     GLAMERVHRELAAAHAKDSTANFTC"
     gene            complement(382..1098)
                     /gene="rph"
                     /locus_tag="VN518_00405"
     CDS             complement(382..1098)
                     /gene="rph"
                     /locus_tag="VN518_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009734676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HXE03064.1"
                     /translation="MRPSKREADALRPISLERGISCHAEGSCLVRFGGTHVLCTASVG
                     ESVPPWLKGGGKGWVTAEYGMLPRATAERTRREAATGKQSGRTQEIQRLIGRSLRAVT
                     DLTALGERQITLDCDVLQADGGTRTASITGAWIALHDAVAWMKARNMLKNGVALRDHV
                     AAVSCGLYGGEAILDLDYAEDSEAQADANFVMTGSGGIVEVQGTAEQQAFSQAQFDQL
                     MALAKRGIAELVQLQKLTVA"
     gene            1265..4048
                     /gene="mutS"
                     /locus_tag="VN518_00410"
     CDS             1265..4048
                     /gene="mutS"
                     /locus_tag="VN518_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HXE03065.1"
                     /translation="MAEAETGAHAERGGFSAGDVPRPDEVGAAGPASIATPMMAQYLE
                     IKSANADCLLFYRMGDFYELFFEDAAIASRALGIALTKRGKHLGEDIPMCGVPIHAAD
                     EYLQRLIAQAHRVAVCEQIEDPAEARKRGPKAVVRRDVVRLVTPGTLTEESLLDARAH
                     NFLTAIFRAPGGEAGEAAFALASLDISTGELLAATVSGSDLAGELARLAPREVLIGDD
                     QAGDPSLRAVIHEAGAALTPIPRAHFDSARGERALKAKLGVAALDAYGEFSKPELAAL
                     AGLFTYVEITQVGRAPLMRPPRKEAPGSLLLIDAATRANLELMRSNQGARSGSLLAAI
                     DRTVTGAGARELASRLGSPLTDAAAINDRLAAISYLAGEPRLRQTLRDELRRAPDLAR
                     ALARLALNRGGPRDLGAVRDAIASARALAAHLARAAGLGLPQELDAVAARLRSVPEDV
                     ENDLAAALADELPLNKRDGGFIRDGFDRALDEHRKLRDGSRQVIAGLQTRYADATGIK
                     SLKVRHNNVLGYFVEVTATNAGALSKPPHVEMFVHRQTLANVMRFSTLELGELEARIT
                     TAADRALALELDIFAKLTERVLAEQASLAGASAGLAELDHHAGLAELAVEQNYARPKV
                     DESPVFAVVEGRHPMVEQNLARGDGASFVGNDCRLGAENKDTGTRILVVTGPNMAGKS
                     TFLRQNALIVVLAQLGSFVPAKSAHVGIVDRLFSRVGAADDLARGRSTFMVEMVETAA
                     ILNQATMRSFVVLDEIGRGTATFDGLSIAWATLEYLHEVNRCRVLFATHYHELTALAE
                     RLPRAANATVEVKEWRDEIVFLYRVVPGAADRSYGIHVAKLAGLPGAVLARANEVLTE
                     LEKADGRPKPSDLASDLPLFSAAKGVSPAQAPRPSPLEATVGALRPDAMTPREALEAI
                     YTLKGLLDRTTEP"
     gene            4078..>6745
                     /locus_tag="VN518_00415"
     CDS             4078..>6745
                     /locus_tag="VN518_00415"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HXE03066.1"
                     /translation="MDQAVVKTAPYFNLDALREETSELACSYSGQDSSLRAALVARLR
                     QLVDDARTEARRQLERDGAGRACAEGLSAFQDALIRLAYDFTTNHVYSAKNPSAAEHM
                     AVVAQGGYGRGMLAPGSDIDLLFLLPYKQTAWGESVAEYMLYLLWDLGFKVGHATRTI
                     DQCVRLSRSDMTIRTALLDARLILGDEVLFAEFQRRFREDVLKTSVRPFVDAKLEEQN
                     ARHARAGASRYLVEPNIKDGKGGLRDLHTLHWLAKHLYPDTAEEEFVEAGVFTPAEYS
                     SFRRCESFLWSVRCQLHFLTERPEERLSFDLQPLMAEKLGYRGHAGLRAVERFMKHYF
                     LVAKEVGELTRIVCSALEMKQLKSLPSLGTLLSPFGWKRRAHLRRTSDFRIENGRISA
                     IHKDVFAKDPVNFIRLFALADEQQATFSPEVLRQVRANLRRVDDSLRANPTANRLFLR
                     LMTDARDPEGVLRSMNEAGVLGHFIPEFGRVVSMMQFNMYHHFTVDEHLIRTIGFLSD
                     IERGKFALEHPVSTEIIRTIDNRRALYVAAFLHDIAKGRDEDHSEVGARIARELGPRL
                     GLTPSEAETATWLVEHHLVMSTFAQSRDLNDPKTIRDFADLVQSRERLKLLLVLTVAD
                     LRAVGPGVWTGWKGQLLRNLYFETEPLLGGGHITLTRSERVTRAQDALREKLADWPKA
                     ELERFIERHYPAYWLRTDLDVIAEHAKLIRDAEAQRKSLVTHIATHEFRGVTEITLLA
                     PNHSRLLAMVAGACAGAGANIVDAQISTTRDGMALDTIQLEREFDHGDDEERRAKKIA
                     TTIERLLKGEVRLADVVATKKKPKARLSAFTVEPQVVIDNTLSDALTVIEINGLDRPG
                     LLYELTATLSKLNLNITSAHVATFGERVVDVFY"
BASE COUNT         1092 a         2370 c         2142 g         1141 t
ORIGIN      
        1 gcaggtgaaa ttggcggtcg agtccttggc atgggcagca gcgagctcgc gatgcacgcg
       61 ctccatggcg agcccgaaat ctcttgccgc tccgccccag cgcgccgaat ggatgcctgg
      121 ctctccgcca agtgccttga cctctagtcc tgaatcgtcg gcaaaggcag gaaggccaga
      181 ggctcgcgct ccggccatgg ccttcagtct ggcattctcg gcgaagctcg cgccggtttc
      241 ctcagtctcc ggcagcccga gcttgcctgc gaacaccgcc tcgacgccga atggcgcgag
      301 cagatcggcg atctcgcgca ccttgccttc attgtggctg gcgatgacga ggcgcgtacc
      361 ccgacgcagc gtgacgccca tctaagcgac ggtgagcttc tgcagttgca cgagctcggc
      421 gatgccgcgc ttggcgagcg ccatcaattg atcgaactgc gcctggctga aggcttgctg
      481 ctcggcggtg ccctgcacct cgacgatgcc tccagagccc gtcatcacga aattggcgtc
      541 ggcctgcgcc tctgaatcct cggcgtagtc gaggtcgagg atggcctcgc cgccataaag
      601 tccacaggac acggcggcaa cgtgatcgcg caaggcaacg ccgttcttca gcatgttgcg
      661 cgccttcatc caggcgacgg cgtcatggag agcgatccac gcgccggtga tggacgcggt
      721 gcgcgtgccg ccgtcggcct ggagcacgtc gcaatcgagc gtgatctgcc gttcgccgag
      781 cgcggtaagg tcggtgacgg cgcggagcga gcggccgatc agccgctgga tctcttgggt
      841 gcggcctgat tgcttgccgg tcgcagcctc ccgccgcgtg cgctcggcgg tggcgcgggg
      901 cagcatgccg tattctgccg tcacccagcc ctttccgccg cctttcagcc atggcggcac
      961 cgactctcca acgctcgccg tgcacagcac gtgggtgccg ccgaaacgga cgaggcagga
     1021 gccttcggcg tggcacgaaa tgccgcgctc gaggctgatg gggcgcaagg cgtctgcctc
     1081 gcgtttggac gggcgcatgc tctctccgtt gaagcccagt ggagcgcgtg aagcgcggcg
     1141 caccctagag gacgcttgcg cgccaagcaa cgggtggaag gggggtgttc gccgctgtgg
     1201 acgattgaaa cggcgagaac ggcctcgctt gtcgggtcgc ctgggagcgg ttaccaccct
     1261 agccatggcc gaggcagaga ccggggctca cgccgaacgg ggcggatttt ccgcgggaga
     1321 cgtcccgcgc cctgatgagg tgggggccgc gggccccgcc agcatcgcca ccccgatgat
     1381 ggcccaatat ctggagatca aatcggccaa cgccgattgc ctcctgttct accgcatggg
     1441 cgatttctac gagctgttct ttgaggacgc tgcaatcgcc agccgcgcgc ttggcatcgc
     1501 gctcaccaag cgcggcaagc acctcggaga ggacatcccc atgtgcggcg tgccgatcca
     1561 tgccgccgac gaatatctcc aacgcctgat cgcgcaagcc catcgcgtcg cggtgtgcga
     1621 gcagatcgag gaccctgccg aggcgcggaa gcgcgggcct aaggccgtgg tccggcgcga
     1681 cgtggttcgc ctcgtcaccc caggcacgct gaccgaggag agcctgctcg acgcccgggc
     1741 gcataacttt ctcaccgcga tcttccgcgc gccggggggc gaggccggcg aggccgcttt
     1801 cgcgctcgcc tctctcgaca tttcaaccgg cgagctgctg gcggcgaccg tgagcggcag
     1861 cgatcttgcc ggcgagcttg cgcggcttgc cccccgcgag gtgctgatcg gtgacgatca
     1921 ggccggcgat ccttcgcttc gcgccgtcat ccacgaggcc ggtgccgcgc tgacgccgat
     1981 cccgcgcgcc catttcgact ctgcccgcgg cgagcgcgcg ctgaaagcga aactcggcgt
     2041 tgctgcgctc gacgcctatg gcgagttctc gaagccagag cttgccgcac tcgccggcct
     2101 cttcacctat gtcgagatca cgcaagtcgg ccgcgcgcca ctgatgcggc caccgcgcaa
     2161 agaggcgccc gggagcctgc tcctgatcga cgccgcgacc cgcgccaatc tcgagctcat
     2221 gcgctcaaac caaggcgccc gctcaggcag cctgctcgct gccatcgacc gcaccgtcac
     2281 cggcgccggc gcgcgcgagc ttgcaagccg cctcggcagc ccgctcaccg atgccgccgc
     2341 catcaacgac cgcctcgccg ccatctctta tctcgccggc gagccgcgat tgcgccagac
     2401 gcttcgcgat gagctgaggc gcgcgcctga cctggcgcgg gcgctggcgc ggcttgcgct
     2461 caaccgcggc ggtccgcgcg atctcggcgc ggtgcgcgac gccatcgcct cggcccgggc
     2521 tcttgccgcg catctcgccc gcgccgctgg cctcggcctg ccgcaggagc tcgatgcggt
     2581 cgctgcgagg ctcaggtcgg tgcctgagga cgtcgagaac gatcttgctg ccgcgctcgc
     2641 cgacgagctg ccgctcaaca agcgcgacgg cggcttcatc cgcgatggct tcgatcgcgc
     2701 tctcgacgag catcggaagc tccgcgacgg cagccgccag gtgatcgccg ggctgcagac
     2761 gcgttacgcc gacgcgaccg gcatcaaatc cctgaaggtg cgccacaaca acgtgctcgg
     2821 ctatttcgtc gaggtcaccg caaccaatgc cggcgctctc tccaagccgc cgcatgtcga
     2881 gatgttcgtg caccggcaga cgctcgccaa cgtcatgcgc ttctccacgc ttgagcttgg
     2941 cgagctcgag gcgcgcatca ccaccgccgc cgaccgcgcg cttgcgctcg agctcgacat
     3001 cttcgccaag ctcaccgaac gcgtgcttgc cgagcaggcc tcgcttgccg gggcgagcgc
     3061 tggcctcgcc gagctcgacc accatgcggg cctcgccgag cttgcggtcg agcagaacta
     3121 cgcgcgaccg aaggtggatg agagcccggt ctttgccgtg gtcgaaggcc gccaccccat
     3181 ggtggagcag aatttggcgc gcggcgacgg cgccagcttt gttggtaatg actgccggct
     3241 gggcgctgag aacaaggaca ccggcacccg catcctggtg gtaaccgggc cgaacatggc
     3301 cggcaagtcc accttcctcc gccagaacgc gctgatcgtg gtgctcgcgc agctcggctc
     3361 cttcgtgccg gcgaagtcgg cgcatgtcgg catcgtcgac cgcctgttca gccgggttgg
     3421 cgccgccgac gatctcgccc gcgggcgctc gaccttcatg gtcgagatgg tggagaccgc
     3481 ggcgatcctc aatcaggcga cgatgcgctc cttcgtcgtg ctcgacgaga tcggccgcgg
     3541 cacggccacc ttcgacgggc tctccattgc ctgggccacg ctcgaatatc tccacgaggt
     3601 gaaccgctgc cgcgtgctgt tcgccaccca ctatcacgag ctgaccgcgc tcgccgaacg
     3661 tctgccccgc gccgccaacg ccacggtgga ggtaaaggag tggcgcgacg agatcgtgtt
     3721 cctctatcgc gtggtgccag gcgccgccga ccgctcttac ggcatccatg tagcgaagct
     3781 cgccggcttg ccaggggccg ttctcgccag agccaatgag gtgctgaccg agctggaaaa
     3841 ggccgacggc aggcctaagc cctccgatct cgcctccgat ctacccttgt tcagcgccgc
     3901 caaaggtgta tcacccgcgc aagccccacg gccgtccccg ctcgaagcca ccgtcggcgc
     3961 gctccggcca gacgccatga cgccgcgcga ggcgcttgag gcgatttaca cgctgaaggg
     4021 cttgttggac cgcactaccg agccttaagt cccaatgtgc taaaattcac gccgcccatg
     4081 gaccaggcag tcgtcaaaac cgcgccttat ttcaatctcg acgcgctccg cgaggagacc
     4141 tctgagctcg cctgctccta ttccgggcag gattcctcgc tccgggcggc gctcgttgcg
     4201 cgcctgaggc agctcgtcga cgacgcgcgg acggaggctc ggcgccagct tgagcgggac
     4261 ggcgccgggc gggcctgcgc cgagggcctt tccgctttcc aggatgccct gatcaggctc
     4321 gcctacgatt tcacgaccaa ccacgtctat agcgccaaga acccctcagc cgccgagcac
     4381 atggccgtcg ttgcccaagg aggctacggc cggggcatgc tggcgcccgg ctcagacatc
     4441 gatctcctgt tcctgcttcc ctacaagcag accgcctggg gcgagagcgt cgccgagtac
     4501 atgctctatc tgctctggga tctcggcttc aaggtcggcc acgccacgcg caccatcgat
     4561 caatgcgtgc ggctgtcgcg ctccgacatg accatccgca cggcgctgct cgatgcgcgc
     4621 ctgatccttg gcgacgaggt gctgtttgcc gagtttcagc gccgcttccg cgaggacgtg
     4681 ctaaagacga gcgtcaggcc cttcgtcgat gccaagctcg aggagcagaa cgcccgtcac
     4741 gcccgcgccg gtgcctcgcg ctatctggtc gagccgaaca tcaaggacgg caagggcggc
     4801 ttgcgcgatc tgcatacgtt gcactggctc gccaagcacc tctatcccga cacggcggag
     4861 gaggagttcg tcgaggccgg tgtgttcacg ccggccgaat acagcagctt ccgccgctgc
     4921 gagagcttcc tctggtcggt gcgctgccag ctgcattttc tcaccgagcg ccccgaggag
     4981 cgcttgagct tcgatcttca gccgctgatg gcggaaaagc tcggctatcg cgggcatgcc
     5041 ggcttgcgcg ccgtcgagcg cttcatgaag cactatttcc tcgtcgccaa ggaagttggc
     5101 gagctcaccc gcatcgtctg ctcggcgctc gagatgaaac aactcaagtc gttgccgagc
     5161 ctcggcacgc ttctgtcgcc gttcggctgg aagcgccgcg cccatcttcg ccgcacctcg
     5221 gacttccgca tcgagaacgg ccgcatcagc gccatccaca aggacgtctt tgccaaggac
     5281 ccggtgaact tcatccgcct gttcgccctt gccgacgagc agcaggccac gttctcgccg
     5341 gaggtgttgc gccaggtgcg ggcgaatttg cggcgggtcg acgactcttt gcgcgccaat
     5401 ccaacggcga accggctgtt cttgcggctg atgaccgatg cccgcgatcc tgaaggcgtg
     5461 ctgcgcagca tgaacgaggc cggcgtgctc ggccatttca tacccgaatt cgggcgcgtg
     5521 gtctcgatga tgcagttcaa catgtatcat cacttcactg tcgatgagca tctgatccgc
     5581 accatcggct tcctgtccga catcgagcgc ggcaagtttg cgcttgagca tccggtctcg
     5641 accgagatca tccgcaccat cgacaatcgc cgcgcgctct acgtcgcggc cttcctgcac
     5701 gacatcgcca agggccgcga cgaggaccat tcggaggtcg gcgcccgcat cgcccgcgag
     5761 ctcgggcctc ggctcggtct cactccgtcg gaagccgaga ccgcgacctg gctcgtcgag
     5821 catcatctcg tgatgagcac cttcgcccag agccgcgacc tgaacgaccc gaagaccatc
     5881 cgcgacttcg ccgatctcgt gcagagccgc gagcggctga agctcctgct cgtgctcacc
     5941 gtcgccgatc tccgtgcagt ggggcctggg gtgtggaccg gctggaaggg gcagctcctg
     6001 cgtaacctct atttcgagac cgagcccctg cttggcggcg gccatatcac gctcacccgc
     6061 agcgagcggg tgacgcgcgc ccaggatgcg ctcagagaga agctcgctga ctggccgaag
     6121 gcggagctcg agcgcttcat cgagcgccac tatccggcct attggctgcg caccgatctc
     6181 gacgtcatcg ccgagcacgc aaagctcatc cgcgatgccg aggcgcagcg gaagtcgctc
     6241 gtcactcata tcgccaccca tgaatttcgc ggcgtcaccg agatcacctt gctcgcgccc
     6301 aaccactccc gcctgctggc catggtggcc ggcgcctgcg ccggggccgg cgccaatatc
     6361 gtcgatgcgc agatctcgac cacgcgcgac ggcatggcgc tcgacaccat ccagctcgag
     6421 cgcgagttcg accatggcga cgacgaagag cggcgggcga agaagatcgc caccaccatc
     6481 gagaggctgt tgaagggcga ggtgcgcttg gccgatgtgg tcgccaccaa gaagaagccg
     6541 aaggcgcggc tttctgcctt caccgtcgag ccgcaggtgg tgatcgacaa caccctgtcg
     6601 gacgcgctga cggtgatcga gatcaacggt ctcgaccggc ccggcctgct ctacgaactc
     6661 accgccacgc tgtcgaagct caacctcaac atcacgtccg cgcatgtcgc gaccttcggc
     6721 gagcgcgtcg tcgacgtgtt ctacg
//
LOCUS       DATNAE010000024         2008 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3935359, whole genome shotgun sequence.
ACCESSION   DATNAE010000024 DATNAE010000000
VERSION     DATNAE010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3935359"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..987
                     /locus_tag="VN518_00420"
     CDS             <1..987
                     /locus_tag="VN518_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HXE03067.1"
                     /translation="SIDLGVLASEPGTLIAVVLVLVAAKAAILYLLGRWWGLDSKGAR
                     RLGLVISQGGEFAFVLFGAGALEGVIDRHMANLLTLAVTLSMAATPLLLLIDDAIARA
                     LKPAPPDYEMPPGGDQHVIIAGFGRFGQIVARVLRARQIPFTALDSNVEQVDFVKRFG
                     AQIYYGDAGRLDILRAAGADKARAFVLAIDDVECSMRVAEMVRTHFPDLPIYARARDR
                     THVHRLMDLGIEIIERETFLAALELTKDLLRGLGLKEPEVKRLTETFKRLDEKRLYED
                     YQYYTDTEKVRANAMSQAKELEELFSRDVEELPDGDGVKLTVLERGDRRSAG"
     gene            complement(947..1750)
                     /locus_tag="VN518_00425"
     CDS             complement(947..1750)
                     /locus_tag="VN518_00425"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXE03068.1"
                     /translation="MKQLSVDGVEIAYLDAGKGPAVIIGHCSSASHKEWLPLIETLKS
                     EWRVLAPDLIGYGQSAPWPADQPFSVGADVDVVLAIAKKAKGKLHLVGHSYGAALLLE
                     AARTLGARVKSLTLVEPRVFHLLRGRPEWAEVETLARAVLGAVASGDDRAAARSFMTY
                     WLGRWRWWLSPERFKAAIEATIPKVALEFGFAVNAPTTLQDYAEVTAPTLLIVGGSTG
                     APNQAIVDLLAATLPDVQVAVVKGARHMSPFTHPAEINRLIVDHLALTR"
     gene            complement(1808..>2008)
                     /locus_tag="VN518_00430"
     CDS             complement(1808..>2008)
                     /locus_tag="VN518_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008551469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HXE03069.1"
                     /translation="GLNARERRVFEARRLAEEPLTLEELSAEFGVSRERIRQIEVRAF
                     EKVQKAVTKAAAAAQPEPAGAE"
BASE COUNT          348 a          657 c          672 g          331 t
ORIGIN      
        1 tcgatcgatc tcggcgtgct ggcttcggag cccggcaccc tgatcgccgt cgtgttggtc
       61 ctcgtcgcgg ccaaggcggc gatcctctat ctgctcggcc gctggtgggg gctggacagc
      121 aagggcgcac gccggctcgg gctggtgatc agtcaaggcg gcgaattcgc cttcgtgctg
      181 ttcggggcag gggccttgga gggcgtgatc gatcggcaca tggccaatct gctcacgctg
      241 gcggtgacgc tgtcgatggc ggccacgccg ctgctgctcc tcatcgacga cgccattgcg
      301 cgcgcgctga aaccagcccc tcccgactat gagatgccgc cgggcggaga tcagcacgtc
      361 atcatcgccg ggttcgggcg cttcgggcag atcgtggcgc gcgtgctgcg cgcccgccag
      421 atcccgttca ccgcgctcga ctcgaacgtc gagcaggtgg acttcgtcaa gcgcttcggc
      481 gcccagatct actatggcga cgccgggcgg ctagacatct tgcgggccgc cggcgccgac
      541 aaggcgcgcg ccttcgtgct cgccatcgac gatgtcgagt gctcgatgcg ggtcgccgaa
      601 atggtgcgca cgcacttccc cgatctgccc atctatgcgc gggcgcgcga ccgcacccac
      661 gtgcataggc tgatggatct cggcatcgag atcatcgagc gcgagacgtt cctggcggcg
      721 ctcgaattga ccaaggattt gttgcgcggc ctcggcctga aagaacccga ggtcaagcgc
      781 ctgaccgaga ccttcaagcg gctcgacgag aagcggctgt acgaggacta tcaatactac
      841 acggataccg agaaggtccg cgccaatgcg atgtcgcagg cgaaggagct cgaggagctc
      901 ttctcgcgcg acgtcgagga gctgcccgac ggcgacggcg tgaagctcac cgtgttagag
      961 cgaggtgatc gacgatcagc cggttgatct cggccggatg ggtgaacggg ctcatatgcc
     1021 gcgccccttt cacgaccgcc acctgaacgt cgggcagtgt cgccgcgaga agatcgacga
     1081 tggcctggtt gggcgcgccg gtcgagccgc cgacaatcag caatgtgggc gcggtgactt
     1141 cggcataatc ttgcaaggtc gttggcgcgt tgacggcgaa gccgaattcg agcgccacct
     1201 tgggaattgt cgcctcgatc gccgctttga agcgctccgg cgacagccac cagcgccagc
     1261 ggccaagcca ataggtcatg aaggacctgg ccgcggcgcg atcgtcgccg cttgccaccg
     1321 cgccgagcac ggcgcgggcc agcgtttcga cctcggccca ttcgggacgc ccgcgcagaa
     1381 gatgaaacac tctcggctcc accagggtaa ggcttttcac gcgggccccg agcgtgcggg
     1441 cggcctccag cagcaaagcc gcgccatagg aatggcccac aagatgcagc ttgcctttcg
     1501 ccttcttggc aatcgcgagc acgacatcca catcggcgcc gacggagaag ggttggtcgg
     1561 cgggccaggg ggcggattgc ccatagccga tcaaatcagg cgcgagcacg cgccactccg
     1621 atttgagcgt ctcgatcagc ggcagccatt ccttgtgcga ggcgctcgag cagtgaccga
     1681 tgatcaccgc cgggcctttg cccgcgtcga gataagcgat ctcgacgcca tcgacgctga
     1741 gttgcttcat gccaagtcga ggaagcagcg gaatggagcg ccgggtcaag cccggcaatg
     1801 agggagatta ctcggcgccg gcaggctcgg gctgcgcggc ggcggcggcc ttggtcaccg
     1861 ccttctgcac cttctcgaaa gcgcgcacct cgatctggcg aatgcgctcg cgggagacgc
     1921 cgaactcggc ggaaagctcc tccagcgtca gcggctcctc ggcaagccgg cgcgcctcga
     1981 acacacgacg ctcgcgcgca ttcaagcc
//
LOCUS       DATNAE010000025         3740 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_327332, whole genome shotgun sequence.
ACCESSION   DATNAE010000025 DATNAE010000000
VERSION     DATNAE010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3740
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327332"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="VN518_00435"
     CDS             complement(<1..743)
                     /locus_tag="VN518_00435"
                     /inference="COORDINATES: protein motif:HMM:NF012730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HXE03070.1"
                     /translation="MSLKSVWRDWRVKVHDILEVGDGNPIGRVVNVFLIVLIVANGLA
                     FAAETIPSLYDRYGPEFEAFNTFSVMVFTVEYVLRLWSSVEIPLLRRLPHWRARLNFA
                     ARPMMIIDLLAILPWYIYLIVPFDLRALRILRLFRLFRLLKLLRYSPALLTLKRVIAH
                     EYRALLGAILLMMMLMLFSAAVIYFLERGAQPDKFGSIPAAAWWALSTLTTVGYGDIV
                     PVTPLGKVFGSIVMLLGLGMFALPIAILSA"
     gene            complement(746..3625)
                     /gene="alaS"
                     /locus_tag="VN518_00440"
     CDS             complement(746..3625)
                     /gene="alaS"
                     /locus_tag="VN518_00440"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HXE03071.1"
                     /translation="MSQAKKTSVKKAKAAGVAGATKKAGKKPMNDKTVTDSSNDAGAS
                     SPSATSVSSTSVNATSVNAIRAGFLDFFARNGHQVLPSSPLVPRHDPTLMFTNAGMVQ
                     FKNVLTGAEKPAFPRVATAQKCVRAGGKHNDLDNVGYTARHHTFFEMLGNFSFGDYFK
                     ERAIALAWELLTKDYALDKKRLLITVCADDDEAARLWRKISGFPDEKIIRIAGSDNFW
                     SMGEFGPCGPSSEIFYDHGPSVQGGPPGSPDAEGDRFVEIWNLVFMQYEQIAADKRVD
                     LPQPSIDTGMGLERIAAVLQGTHDNYEIDLFRALIAASVSLTGVPAKGEAKPSHRVIA
                     DHLRAVSFLIADGVLPSNEGRGYVLRRIMRRAMRHAHLLGAEEPLLWRLVPTLVTEMG
                     HAYPELERGQSLIVETLKLEETRFSDTLERGLRLLDEATRGLKRGGMLPGETAFKLYD
                     TYGFPFDLTEDALKARGIGVDTKGFAACMERQRAEARKSWAGSGEAATETLWFELKEE
                     VGATEFLGYGAEASSGEIVALVKDGKRVSELKAGERGAVIVNQTPFYGESGGQVGDRG
                     LVTSPSGARFSVEDTQKKLHGLFLHSGALQAGTLKVGEVVDLEVDHARRTATRANHSA
                     THLLHEALREVLGLHVAQKGSLVEPSRLRFDFSHPRPMTQAEIKAVEDLANTMVLQNS
                     AVETHLMGPDEAIAKGALALFGEKYGEEVRVVSMGRSEASHKADHAYSVELCGGTHVR
                     RTGDIGLFKIVGESAVASGVRRIEALTGEGARSYLATQDQRVREAAELLKVGPDEMIE
                     RLAAILDERRKLEKQLADAKRELALGGGAAADTASAIRDLGPVKLFARTLQDVAPKDL
                     RGLVDDAKRQLGSGVVAIIGVSEEGKAGLVVGVTDDLTQTYNAVELARAGAEALGGKG
                     GGGRPDLAQAGGPDGANAAAALDAIAARIAGGAAAEADAARAGTA"
BASE COUNT          664 a         1210 c         1243 g          623 t
ORIGIN      
        1 gcggacagaa tggcgatggg aagggcgaac atgccgaggc cgagcagcat cacgatgctg
       61 ccgaatacct tgccgagcgg cgtcaccggc acgatgtcgc catagccgac ggtggtgagc
      121 gtcgacaagg cccaccacgc agcggcggga attgagccga acttgtcggg ctgcgccccg
      181 cgctcgagga aatagatgac ggccgccgag aacagcatca gcatcatcat gaggaggatc
      241 gcgccaagca gcgcgcgata ttcgtgagcg atgacgcgct tcagggtcag cagcgccggc
      301 gaatagcgca gcaatttgag cagccggaac aaccggaaca ggcgcaagat ccgcaaggcg
      361 cgcagatcga acggcacgat caggtaaata taccagggca ggatagcgag caggtcgatg
      421 atcatcatcg gcctcgccgc aaagttcagc cgcgcgcgcc aatgcggcag ccgacgcaac
      481 aacgggatct cgaccgagct ccacaggcgc agcacatatt cgacggtgaa caccatcacc
      541 gagaaagtgt tgaacgcctc gaactcgggc ccatagcgat catagagaga agggatcgtc
      601 tcggcggcaa aggcgaggcc gttggcgacg atgagaacga tgaggaagac gttgacgacg
      661 cggccgatcg gattgccgtc gccgacctct aagatgtcgt gaaccttgac ccgccagtct
      721 cgccagacgg atttgaggct catcgtcagg cggtccccgc gcgggccgcg tccgcctcgg
      781 cggcagcgcc gccggcgatc cgcgccgcga tggcatcgag cgcggcggcg gcgttggcgc
      841 catcaggccc tccggcctgg gcgaggtccg ggcgaccccc cccgcctttg ccgccaagcg
      901 cctcggcgcc ggcgcgggca agctcgaccg cgttgtaggt ttgggtgagg tcgtcggtga
      961 cgccgaccac gaggccggcc ttgccttcct ccgacacgcc gataatggcg accacgccag
     1021 aaccgagctg gcgcttggcg tcatcgacca gcccgcggag atccttaggc gccacgtcct
     1081 gcagcgtgcg ggcgaacagc ttcaccgggc caagatcgcg gatcgcggat gccgtatcgg
     1141 ccgccgcacc gccgccgagc gcaagctcgc gcttggcatc ggcgagctgc ttctcgagct
     1201 tccgccgctc atcgaggatg gcggcgagcc gctcgatcat ctcgtcgggg ccgaccttga
     1261 gcagctccgc tgcctcgcgc acgcgctgat cctgcgtcgc aagataagag cgagcgccct
     1321 cacccgtgag cgcctcgatg cggcggacgc cggacgcaac cgcgctctcg ccaacgatct
     1381 tgaagaggcc gatatcgccg gtgcggcgca catgcgtgcc gccgcacagc tcgacggagt
     1441 aagcgtgatc ggccttgtgg ctcgcctcgc ttctgcccat gctgacgacg cggacctcct
     1501 cgccgtattt ctcgccgaac agcgccagcg cccccttggc gatggcctcg tcggggccca
     1561 tcagatgggt ctcgaccgct gagttctgca gcaccatggt gttggcgaga tcttccaccg
     1621 ccttgatctc ggcttgcgtc atcggccgcg gatgggagaa atcgaatctg agccgggaag
     1681 gctcgaccag cgaacccttc tgcgccacgt gaagcccaag cacctcgcgc agcgcctcgt
     1741 gcaagagatg cgtcgcggag tgattggcgc gcgtcgcggt gcggcgggca tgatcgactt
     1801 cgagatcgac gacctcgccg accttcaacg tgccggcttg aagcgcaccg gaatggagaa
     1861 acaggccatg cagcttcttc tgcgtgtcct cgacgctaaa gcgcgcgcct gacgggctcg
     1921 tgaccagccc gcggtcgccg acctggccgc cggactcgcc gtagaatggc gtctggttga
     1981 cgatgaccgc accgcgctcg ccggctttga gctcagacac gcgcttgccg tccttgacga
     2041 gcgccacgat ctcgccggac gaggcctcgg cgccgtagcc cagaaactcg gtggcgccca
     2101 cctcctcctt cagctcaaac cacaaggtct cggtcgcggc ctcgcctgag ccagcccagg
     2161 actttcgcgc ctcggcgcgc tgccgctcca tgcatgcggc gaagcccttg gtgtcgacgc
     2221 cgatgccgcg ggctttcagc gcatcctcgg tcaggtcgaa ggggaagccg taagtgtcgt
     2281 aaagcttgaa cgcggtctcg ccgggaagca tgccgccgcg cttcaatccc cgcgtcgcct
     2341 cgtcgagcag gcgaagcccg cgctccagcg tatcgctgaa gcgggtttcc tcgagcttca
     2401 gcgtctcgac gatgaggctc tggccgcgct cgagctccgg gtaggcgtgg cccatttcgg
     2461 tgaccagcgt cggcaccagc cgccacagca gcggctcctc ggcgccgagg agatgtgcgt
     2521 gacgcatggc gcggcgcatg atgcggcgga gcacgtagcc gcgtccctcg ttcgacggca
     2581 gcacgccgtc ggcgatgagg aaggagaccg cgcgcaaatg atcggcgatg acgcggtggc
     2641 ttggcttggc ctcgcccttg gccggcacgc cggtgagcga gaccgaggct gcgatcagcg
     2701 cacggaacag gtcgatctcg taattgtcgt gggtgccttg caggacggcc gctatgcgct
     2761 caagccccat gccggtgtcg atggagggct gcggcaggtc gacgcgctta tccgcggcga
     2821 tctgctcgta ctgcatgaac acgagattcc aaatctcgac gaagcggtcg ccttcggcgt
     2881 cggggctgcc gggaggaccg ccttgcacgc tcgggccgtg atcgtagaaa atctccgagg
     2941 acggaccgca aggtccaaac tcgcccatcg accagaaatt gtcggagcct gcgatgcgga
     3001 tgatcttctc gtcggggaag ccggagattt tgcgccacag ccgcgccgcc tcgtcgtcgt
     3061 cggcacagac ggtgatcaag aggcgcttct tgtcgagcgc atagtccttg gtgaggagct
     3121 cccaggcgag cgctatcgcg cgctccttga aataatcgcc gaaggagaaa ttgccgagca
     3181 tctcgaagaa ggtgtggtgg cgcgcggtgt agccgacatt gtcgaggtcg ttgtgcttgc
     3241 cgccggcgcg cacgcatttc tgcgccgtgg cgacgcgcgg gaaagcaggc ttctcggcgc
     3301 cggtgagcac gttcttgaac tgcaccatgc cggcattggt gaacatcaag gtggggtcgt
     3361 ggcgcggcac gagcgggctc gatggcagca cctgatgacc gttgcgggcg aagaaatcga
     3421 gaaagcccgc gcggatcgcg ttcacactcg tggcgttgac actcgtgctg ctgacactgg
     3481 tggcgctggg actcgaagcg cccgcgtcgt tggagctatc ggtaaccgtc ttgtcgttca
     3541 tgggcttttt gccggctttc ttagtggctc cggccacgcc ggcggccttg gctttcttga
     3601 cgctcgtctt cttggcttgg ctcatggcag cgatgctggg aagccttaag ttgcgagcga
     3661 ggctacgcga agattgcaaa cgaatcctag cctaaactgg cggctgccaa gctggcacgc
     3721 gccgctctgg aacctcaaag
//
LOCUS       DATNAE010000026         3538 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3337170, whole genome shotgun sequence.
ACCESSION   DATNAE010000026 DATNAE010000000
VERSION     DATNAE010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3337170"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="VN518_00445"
     CDS             <1..561
                     /locus_tag="VN518_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03072.1"
                     /translation="GDGDGRPVIGMFLGGAGLADTMLYCRDKIYPLGLPNGIAHGITA
                     LAAIAKQILRIRASFLPPDSKPISLTLSHQGGSLSGRFSLLAVTTLEKLLLSTNIGAH
                     RQGTLKLLAIEERPRSVVHALLAKIAGKLGRSNVDGVHIKEAHEITIEGESSELILDG
                     ESFRTEVGRPISLTPAHPLSFVKLAA"
     gene            696..1565
                     /locus_tag="VN518_00450"
     CDS             696..1565
                     /locus_tag="VN518_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03073.1"
                     /translation="MADDSALRILVAEELSLPVDPRVSEVAGAIAAKHRAASRSLLFY
                     GSCLREERLDGLMLDFYLLVSDYHAAYGKRWLAIANRLIPPNVFYLEHNGLVSKYAVL
                     SEADFHRLAGPETSSVSVWARFAQPSRLVWTADEAARERAIDAVSRAAPTLLAAARPK
                     PGEEALGLWRRAFALTYSAELRAERKGRATSVVDLDPKRYERFSAPALDAAQSIRPHR
                     NWAWRRVEGKALSVARLAKASLTFAGGADYIAWKINRHAGADIRLKPWQRRHPLLAGI
                     SLLPRLLRSGAVR"
     gene            complement(1522..3507)
                     /locus_tag="VN518_00455"
     CDS             complement(1522..3507)
                     /locus_tag="VN518_00455"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXE03074.1"
                     /translation="MMGDGEDVRFDWRRGGAAAAGLVAALVLVIMVFAVKFANDARER
                     ALTAERQANQVALIVRNASANISRAQAALARFVLDEQVKPNGNIYASDWQLAGYQIEQ
                     LKDLERKSPEQLRRVYDAQALYNKLGGELALAARAALAKQGDTGISYFYAAAKTGTSN
                     ALDDKLEEIIRAEREARAGSVEQSQFSSNQADQLTNYLSWLGIIVGLGAIFLGVVAVQ
                     ALRQNAQARQEAESEAERAEALEYAVRERTQELWEANQALKAEAAEREAAEAQLRQVQ
                     KMEAVGQLTGGIAHDFNNMLAVVVGGIDLALRRLNGPRREVMMHLNNAMEGATRAAAL
                     TRRLLSFARSEPLLPERVEPNELILGMSDLLDRTLGERIHIEIDIEPDAWPAYVDPHQ
                     LENAIVNLAVNARDAMEGEGSMRIATENVTMAASQVGDIRAGDYVKISVTDTGCGMPP
                     DILEHAFEPFFTTKPVGKGTGLGLSQIFGFAHESGGEVGIESEVGRGTAVSIYLPRTN
                     AAATVRLHPAAQRTDTADVHVLGARILLVEDDPRVRTATVGALEDLDYEPAACGSGAE
                     AIELFNSQMFDLVITDVIMPEMTGPELIRYLKSNGSHDFAVLFVTGYVGEGESDDLRD
                     YELLRKPFTVGALANAVAAALARTTEPPRSSAGAARG"
BASE COUNT          584 a         1133 c         1173 g          648 t
ORIGIN      
        1 ggcgacgggg acgggcgccc ggtcattgga atgttcctcg gcggcgccgg ccttgccgac
       61 acaatgcttt attgccgcga caagatctat ccgctcggcc tgccgaacgg aatcgcgcac
      121 gggatcactg cgctcgccgc gatcgccaag cagatcctgc gcatccgcgc aagcttcctc
      181 ccgccggatt cgaagccgat aagcttgacg ctatcacatc agggcgggag cctcagcggg
      241 cgcttctcgc tactggcggt gacgacactc gaaaagctac tcttgtcgac caacatcggc
      301 gctcaccggc agggcacgct gaagctgctg gcgattgagg aacggccaag gtcggtggtc
      361 cacgcactgc tggcgaagat cgccggcaag ctcggccgct cgaacgtcga cggggtgcat
      421 atcaaggaag cgcacgagat caccatcgag ggcgagagct ccgagctgat cctcgacggt
      481 gagagcttcc gcaccgaggt cgggcggccg atcagcctca ccccagcgca cccgctttcg
      541 ttcgtcaagc tggcggcctg acggcggcgt ttgcttcgcc gcttatcccc aagtagcagc
      601 cgagcgaagt cgagcttggg tatcgaggga cggtccttcg acaggcgact tcgatgcgct
      661 acgcttgatc attgcctagt cgaggatgag cggaaatggc tgacgacagc gcccttcgca
      721 ttctcgtcgc cgaggagctg agcctgcccg tcgatccgcg ggtgagcgaa gtggctgggg
      781 cgatcgcggc gaagcatcgc gcggcgagcc gctcactgtt gttctacggc tcatgcctgc
      841 gcgaggagcg gctcgacggg ctgatgctcg atttctatct gctcgtgtcc gattatcacg
      901 cggcttatgg caagcgctgg ctggccattg cgaaccggct catcccgccg aacgttttct
      961 atttggagca caatgggctc gtctccaaat atgcggtgct gagcgaagcg gattttcatc
     1021 ggctcgccgg accggaaacg agcagcgtgt cggtttgggc gcggttcgcg caaccgtcac
     1081 ggctggtatg gacggcggac gaagcggcac gcgagcgagc catcgacgca gtctctcggg
     1141 ccgcgccgac actgctcgcc gcggcccggc ccaagccggg tgaggaggcg ctggggctgt
     1201 ggcggcgagc gttcgctttg acctactcgg cggaactgcg tgccgagcga aagggccggg
     1261 cgacttcagt ggtcgatctc gatcccaagc gatatgagcg attttcggcg ccggcgctgg
     1321 atgcagcgca atcgatccgc ccgcaccgca attgggcgtg gcggcgggta gagggcaagg
     1381 cgctgtcagt tgcgcgtctc gccaaggcga gcctgacctt cgccgggggc gccgactata
     1441 tcgcgtggaa gatcaaccgc cacgcggggg ctgacatccg gctgaaaccc tggcagcggc
     1501 ggcaccctct gcttgcgggg atcagcctct tgccgcgcct gctgaggagc ggggcggttc
     1561 ggtagttcgg gcaagcgccg cggcgacggc attggcaaga gcgccaacgg tgaacggctt
     1621 tctcaacagc tcatagtcgc gaagatcgtc ggactcgcct tcgccgacgt aaccggtgac
     1681 gaacagcacg gcgaagtcgt ggctgccgtt cgattttaga tagcggatga gctcgggacc
     1741 ggtcatctcc ggcatgatca cgtcggtgat gacgaggtcg aacatctggg aattgaacaa
     1801 ttcgatcgcc tcggcgccac tgccgcaggc tgcgggttcg taatcgagat cctcgagcgc
     1861 accgaccgtc gcagtgcgca ctcggggatc gtcctcgacc agaaggattc gggcgcccaa
     1921 gacgtggacg tcggcggtat cggtgcgctg cgccgccggg tgcagccgca cggttgcagc
     1981 agcgttcgtg cgcgggaggt agatggaaac ggctgtgcct ctgccgactt cgctctcgat
     2041 cccgacctcg ccgccggact catgcgcgaa accgaaaatc tggctcaggc ccaggcccgt
     2101 gcccttgccg accggcttgg tggtgaagaa cggctcgaac gcgtgctcga ggatatcggg
     2161 cggcattccg cagccggtgt cggtgacgct gatcttcaca taatcgccgg cgcggatgtc
     2221 gccgacctgg ctcgccgcca tcgtcacatt ctcggtcgcg attcgcatgc tgccttcgcc
     2281 ctccatcgca tcgcgggcgt tgaccgcgag attgacgatc gcattctcga gctggtgcgg
     2341 gtcgacatag gccggccaag cgtcaggctc gatgtcgatc tcgatgtgga tccgctcgcc
     2401 gagcgtgcgg tcgagcaggt cggacatgcc gaggatcagc tcgttgggtt cgacccgctc
     2461 gggaagcaaa ggttcggacc gggcgaagga caagagtcgg cgggtcagcg ccgcggcgcg
     2521 ggtcgcgcct tccatcgcat tgttgaggtg catcatcacc tcgcgccgag ggccgttgag
     2581 gcgccgcagc gcgaggtcga tcccgccgac gacaaccgcg agcatgttgt tgaagtcgtg
     2641 cgcgattccg cccgtgagct ggccgacggc ctccatcttc tggacctggc gaagctgcgc
     2701 ctcggcggct tcgcgctcgg ctgcctcggc cttgagggcc tggttcgcct cccacagctc
     2761 ctgcgttcgc tcgcggaccg catattcgag cgcttcggcg cgctcggcct cgctctctgc
     2821 ctcttggcgg gcctgggcat tctggcgcag cgcctggacc gcgaccacgc cgagaaaaat
     2881 tgcgccgagc ccgacgatga ttccgagcca gctgagataa ttggtgagct gatctgcctg
     2941 gttggacgaa aattggcttt gctcgacgct tcctgcacgg gcctcccgct cggcgcggat
     3001 gatctcctcg agcttgtcgt cgagcgcgtt gctggtcccc gtcttggctg cggcatagaa
     3061 atagctgatc ccggtgtcgc cctgcttcgc gagcgccgcc cgggctgcga gcgcaagctc
     3121 tccgccaagt ttattgtaga gcgcctgcgc atcatagacg cgccgcagct gctcgggact
     3181 cttgcgctcc aaatccttca gctgctcgat ctggtagccg gcgagctgcc agtcgctcgc
     3241 ataaatattg ccgttcggct tcacctgctc gtcgagaacg aaacgcgcga gcgcggcttg
     3301 agcccgggag atattggcgc tggcgttgcg gacgatcagc gcgacctggt tggcctggcg
     3361 ctcggcggta agcgcacgtt cgcgcgcatc attggcgaac ttcaccgcga acaccatgat
     3421 gaccaggacg agggccgcaa cgagtcccgc cgccgcggca ccgccgcgcc gccagtcgaa
     3481 ccggacgtcc tccccgtcgc ccatcatcca gccaatgtag tcgcgttaac ctcttgac
//
LOCUS       DATNAE010000027         3445 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3937804, whole genome shotgun sequence.
ACCESSION   DATNAE010000027 DATNAE010000000
VERSION     DATNAE010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3445
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3937804"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /gene="ychF"
                     /locus_tag="VN518_00460"
     CDS             complement(<1..601)
                     /gene="ychF"
                     /locus_tag="VN518_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HXE03075.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTESAAAQAANYPFCTIEPNVGE
                     VPVPDPRLDRLAAIAKSAQVIATRLTFVDIAGLVRGASKGEGLGNQFLAHIREVDAIA
                     YVLRCFEDPGVVHVEGRIDPIDDADTIETELMLADLDSLERRIPLLEKKLRGQDKEAK
                     KTLELVERILPLLREGKPARLASVADEERSAFNALNLLTS"
     gene            complement(616..1308)
                     /gene="pth"
                     /locus_tag="VN518_00465"
     CDS             complement(616..1308)
                     /gene="pth"
                     /locus_tag="VN518_00465"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HXE03076.1"
                     /translation="MKLFVGLGNPGAEHALNRHNVGFMAVDAIAAAHGFPAWRKRFQG
                     AASEGKLGGEQVLLFKPGTFMNASGRAVGEALRFYKLELADVAVFHDELDLAPGKVRV
                     KTGGGVSGHNGLRSLSAHIGNDYVRVRIGVGHPGQRDLVVGHVLHDFAKADYAWLEPV
                     LGAIAAEAPYLAEGDTERFQSRVAHAVQAERPAKPAPRAKPGPRGKEPPVEKKAEPQG
                     SLAEKLRRWFGA"
     gene            complement(1366..2064)
                     /locus_tag="VN518_00470"
     CDS             complement(1366..2064)
                     /locus_tag="VN518_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007009899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HXE03077.1"
                     /translation="MAESVELKAWARQRSGKGGARSTRREGRIPGILYGDKKDPESIA
                     VEYRAITQQLLTGHFQSTVFTLNVDGTKTRVIPRAVQLDPIRDYPIHVDFLRLSKDAL
                     VTVEVPVHFLNEAASPGLKRGGVLNVVRHEIQVRCPSDAIPDHFDVDLTGLEIGDAVH
                     ISAIKLPEGVRPTTTDRDFTVATIVGRSAEEPVAGAPTAEAAVPGAEGAEAAPAEGAE
                     DKEKPKEETKKKKE"
     gene            2289..2504
                     /locus_tag="VN518_00475"
     CDS             2289..2504
                     /locus_tag="VN518_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HXE03078.1"
                     /translation="MPQSVYKVIEVIGTSPKSWEEAAKNAVELTGEHLRELRVAEVVE
                     QDLVITNGKVEAYRTKLKVSFKYEGGH"
     gene            complement(2521..>3445)
                     /locus_tag="VN518_00480"
     CDS             complement(2521..>3445)
                     /locus_tag="VN518_00480"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HXE03079.1"
                     /translation="VAGNSNRPLAEAIAGYLKTPLAKCIVRRFADMEIFVEVQENVRG
                     EDVFVIQSTSAPANDHLMELLIIIDALKRASARRITAVVPYFGYARRDRKPGPRTPIS
                     AKLVANMIVEAGADRVLTLDLHAGQIQGFFDIPTDNLFAAPVMVQDIKQLFPADNLMV
                     VSPDVGGVVRARGLAKRIDAPLAIVDKRRERPGDSEVMNVIGNVKGRTCILVDDIVDS
                     GGTLVNAAEALLDHGATLVTAYITHGVLSGGAVARIMSSRLKELVITDSILPSDSVKN
                     AHNIRVLPIASLLGEAIARTAEEQSVSSLFY"
BASE COUNT          589 a         1131 c         1136 g          589 t
ORIGIN      
        1 tggaggtgag caggttgagc gcgttgaagg cggagcgctc ctcgtcggcg acggaggcga
       61 gccgggcggg cttcccctcg cgcaacagcg gcaaaatgcg ctccaccagt tcgagcgtct
      121 ttttcgcctc cttgtcctgg ccgcgcagct tcttctccag cagcggtatc cgccgctcca
      181 gactgtcgag atcggccagc atcagctcgg tctcgatcgt gtcggcatcg tcgatcggat
      241 cgatgcggcc ctcgacatgc accacgccgg gatcctcgaa gcagcgcagc acataggcga
      301 tggcgtcgac ttcgcgaata tgcgccagga actggttacc gagcccttcg cccttggatg
      361 cgccgcgcac cagcccggcg atgtcgacaa aggtaagccg cgtggcgatc acttgcgcgg
      421 acttggcgat ggccgccagc cggtcgaggc gcgggtcggg aaccggcacc tcgccgacat
      481 tcggctcgat ggtgcagaat ggataattgg ccgcctgcgc cgccgcgctc tcggtcagcg
      541 cgttgaacag ggtggacttg ccgacattgg gcagtccgac gatgccgcat ttgaatccca
      601 tgggtcagtc cgccttcacg cgccgaacca gcggcgcagc ttctccgcca ggctaccttg
      661 cggctcggcc ttcttctcga ccggcggctc cttgcctcgc gggccaggct tcgcgcgcgg
      721 ggcgggcttg gcggggcgct cggcttgcac cgcgtgcgcc acgcgcgact ggaacctctc
      781 ggtgtctcct tcggcgagat aaggagcctc cgcggcgatc gcgccgagca caggctcgag
      841 ccacgcataa tccgccttgg cgaaatcgtg cagcacgtga ccgacgacaa ggtccctttg
      901 cccgggatgg ccgacgccga tgcgcacccg gacatagtcg ttgccgatat gcgccgagag
      961 cgatctgagc ccgttatggc cggagacgcc gccgcccgtc ttcacccgca ccttgcccgg
     1021 cgcgaggtcg agctcgtcat ggaacaccgc cacatcggcg agctcgagct tgtagaagcg
     1081 cagcgcctcg cccacggcgc ggccggaggc gttcataaaa gtcccaggct tgaacaggag
     1141 gacctgctcg cctccgagct ttccttccga ggctgcgccc tgaaagcgct tgcgccaggc
     1201 gggaaagcca tgcgccgcgg cgatggcgtc gaccgccatg aagcccacat tgtggcggtt
     1261 gagcgcgtgt tccgccccgg gattgccgag gccgacaaag agcttcatcg ggatcgttcc
     1321 tgccgaaggt gaaacgcgca agcgctcgat cgcgccagaa ggatgttact ccttcttctt
     1381 cttcgtctct tccttcggtt tctccttgtc ctcggcgccc tcggcgggag cagcctcggc
     1441 accctcggcc ccgggcacgg cagcctcggc cgtgggcgcg ccggccaccg gctcttcagc
     1501 cgagcggccg acgatggtcg ccacggtgaa gtcgcgatcg gtggtggtcg ggcgcacccc
     1561 ctcgggcaac ttgatggcag agatgtgcac cgcgtcgcca atctccagcc cggtcaggtc
     1621 gacgtcgaaa tgatcgggaa tggcgtcgga ggggcagcgc acctggatct cgtggcgcac
     1681 cacattgagc acgccgccgc gcttcaagcc gggggaggcg gcctcgttca ggaaatgcac
     1741 cggcacctcg acggtgacca gggcgtcctt gctgagccgc aggaaatcga catggatcgg
     1801 ataatcccgg atgggatcga gctgtaccgc gcgggggatc acccgcgtct tggtgccgtc
     1861 gacgtttagt gtgaagacgg tgctctggaa atggccggtg agaagctgtt gcgtgatcgc
     1921 ccgatactcg accgcgatcg actccgggtc cttcttgtcg ccgtaaagga tgccgggaat
     1981 gcgcccttca cgccgcgtgg aacgcgcccc gcctttgcct gagcgctgcc tcgcccatgc
     2041 cttgagttcg actgactcag ccatgctagc ctccaaagcc aaaaaatgaa gggcccaaag
     2101 aggcccgcga attgagggcg cttatagccc agagcgccgc cggatgacaa cactcctatc
     2161 ggcgcgaggg gcccggctcc gtttggagcc agggccgcat taggcgtaga ctgaatttat
     2221 cagcgcgcgt tcggcggtcc aaatccgtca gtgtcgcgag ccccccgcgt cgaaggagta
     2281 aagtcgccat gccacagagc gtctacaaag tcatcgaagt gatcggcacc agcccgaaat
     2341 cctgggaaga agccgccaag aacgcggtcg agcttaccgg cgagcatctg cgggagctgc
     2401 gtgtggccga ggtcgtcgag caggacctgg tcatcaccaa cggcaaggtc gaggcctacc
     2461 gcaccaagct caaggtctcc ttcaaatatg agggcggcca ctgacgcggc gaggcaagcc
     2521 ttagtagaac aggctcgaga ccgactgctc ctcggccgtg cgcgcgatcg cctcgccgag
     2581 cagggaggcg atcggcagca cgcggatgtt gtgcgcgttc ttcaccgagt cgctcggcag
     2641 gatggagtcg gtgatgacca gctccttcag ccgcgacgac atgatgcgcg ccaccgcgcc
     2701 gcccgacagc acgccgtggg tgatataggc ggtgaccagc gtggcgccgt gatcgagcag
     2761 cgcctcggcc gcgttgacca gcgtgccgcc ggagtcgacg atgtcatcga ccaggatgca
     2821 ggtgcggccc ttcacgttgc cgatcacgtt catcacttcc gaatcgccgg gacgctcgcg
     2881 ccgcttgtcg acgatggcca gcggtgcgtc gatgcgcttg gccagtccgc gcgcgcgcac
     2941 cacgccgccc acgtcgggcg acaccaccat gagattgtcg gcggggaaca actgcttgat
     3001 gtcctgcacc atcaccggcg cggcgaacag attgtcggtg gggatgtcga agaagccctg
     3061 gatctgcccg gcatgcagat cgagcgtgag cacgcggtcg gcgccggcct cgacgatcat
     3121 gttggcgacg agcttggccg agatcggcgt gcgcgggccc ggtttccggt cccgccgcgc
     3181 ataaccgaaa taggggacca ccgctgtgat gcgccgcgcc gatgcgcgct tcagcgcatc
     3241 gatgatgatc agcagctcca tcaggtggtc gttggcgggc gccgaggtcg actggatgac
     3301 gaacacgtct tcgccgcgca cgttctcctg cacctcgacg aagatctcca tgtcggcgaa
     3361 gcgccgcacg atacatttgg cgagaggggt cttgaggtag cctgcgatcg cctcggcgag
     3421 ggggcggttg ctattgcccg caacc
//
LOCUS       DATNAE010000028         5989 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_1533080, whole genome shotgun sequence.
ACCESSION   DATNAE010000028 DATNAE010000000
VERSION     DATNAE010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5989
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1533080"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1281)
                     /locus_tag="VN518_00485"
     CDS             complement(<1..1281)
                     /locus_tag="VN518_00485"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HXE03080.1"
                     /translation="MSEETVYKVQKDWKKNAFIDKDEYEKLYEKSVEKPDKFWGKQGE
                     RIDWIKPYSIVKNTSFAYPDISIKWFEDGTLNVSANCIDRHLKKREHQVAIIWEPDDP
                     AEPPRHITYGELYREVNIFANVLKSVGVKRGDRVTIYLPMIPEAAFAMLACARIGAIH
                     SVVFGGFSPDSLVGRITDCKSKLVITADEGLRGGRKVKLKHNADEALTKSLGDEKVIV
                     IKRTGGQCAMHAGRDFWYDDLAAKVSDHCPPEEMGAEDPLFILYTSGSTGQPKGVVHT
                     TGGYLVYTSLTHQLIFDYHDGDVYWCTADVGWVTGHSYIVYGPLANGATTLMFEGVPN
                     HPDPSRFWQVVDKHGVNIIYTAPTAIRALMGAGDDYVTHTSRKSLRVLGSVGEPINPE
                     AWEWYYRVVGEERCPIVDTWWQTETGGILITPLPG"
     gene            complement(1394..1795)
                     /locus_tag="VN518_00490"
     CDS             complement(1394..1795)
                     /locus_tag="VN518_00490"
                     /inference="COORDINATES: protein motif:HMM:NF020157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HXE03081.1"
                     /translation="MPAFSINYLAVVIAAIAGFLVGWGWYTIFGKVWQDALGKKKEDC
                     KPTPLPFIIAAIGCLLMAWMLAGLMGHLADVTMRGGLISGFFVWVGFVLTTTATNQAF
                     HGTKPIVTVIDAAHWLVVLAVMGAIIGAFGA"
     gene            complement(1814..2242)
                     /locus_tag="VN518_00495"
     CDS             complement(1814..2242)
                     /locus_tag="VN518_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004862538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HXE03082.1"
                     /translation="MNYWLFKSEPDAWSWDQQKNEGAKGGEWDGVRNYQARNVMRSMK
                     KGDLGFFYHSGEEKRIVGVVKVVAEAHPDSTDGTGVWECVDVAAMSDVKRPVTLAEIK
                     ANKALKDMVLVNNSRLSVQPVSAEEWSIISRLVGLKEKSR"
     gene            complement(2253..2543)
                     /locus_tag="VN518_00500"
     CDS             complement(2253..2543)
                     /locus_tag="VN518_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006201141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HXE03083.1"
                     /translation="MLYALICTDKPNSLALRKAKRPEHVAYLKGLGGMLKLAGPFTEA
                     DGETMNGSLVVIEAASLQAAKEIAAGDPHAKAGLFASVEIRPWIWTLNNPSQ"
     gene            complement(2605..3597)
                     /locus_tag="VN518_00505"
     CDS             complement(2605..3597)
                     /locus_tag="VN518_00505"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009734757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HXE03084.1"
                     /translation="MALQSVGIVGGGAWGTALAVSARRAGRDVMLWAYEPETIAEINQ
                     QHRNALYLPGVSLDPAIKATAKLNEIAACDLVLMVTPAQEVRAIADELAPHMRTDQPL
                     VIGAKGIEQSTGQLLSQVVAEVLPQVRTAVLSGPSFAAEIARGLPAALTLATASEAHG
                     RALAHALSHASFRCYWSDDVAGAEIGGALKNVYAIAAGIVVGKKLGASAHAALVTRGF
                     AEMMRFGGSLGARRETMTGLSGLGDLILTCGSPQSRNMSLGSALGEGRSLGEVLKSRL
                     SVTEGVYTASAVVEIAARGIEMPIAQAVHAVVSGLATVDEAIEALLARPLRPEG"
     gene            3877..4086
                     /locus_tag="VN518_00510"
     CDS             3877..4086
                     /locus_tag="VN518_00510"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXE03085.1"
                     /translation="MIKAQTIEIVNAVSSGQCVVLEAIWTGTLAVGFGTLKPGDQLRA
                     RFAQVFEFKDGLIWRQRNYDCFEAW"
     gene            complement(4087..5181)
                     /gene="tsaD"
                     /locus_tag="VN518_00515"
     CDS             complement(4087..5181)
                     /gene="tsaD"
                     /locus_tag="VN518_00515"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HXE03086.1"
                     /translation="MATVPEKRAFVVLGIETSCDETAAALVRREASGKGVLLANLVRS
                     QLALHAEFGGVVPEIAARAHVELLDRLIAEALAESRLGWEEIDGVAATAGPGLIGGLI
                     VGVTTAKAIALARGLPFIAVNHLEAHALTTGLTHGLRFPYLLLLVSGGHTQLLIVKGV
                     GSYTRLGTTLDDALGEAFDKAAKLLGLGYPGGPAVEECAKRGRATVALPRPMLGRSEP
                     HFSLAGLKTALRHAAMKREPLGEQDVADLCASFQEAAADVVSDRTGRAMELYVDVLGP
                     DAQKALVVAGGVAANQRLRAALADAAAKQGFALILPPAELCTDNAAMVAWAGAERLVR
                     GLVDDLAAPSRARWPLDPDAAPAIGAGVKA"
     gene            5263..>5989
                     /gene="hemC"
                     /locus_tag="VN518_00520"
     CDS             5263..>5989
                     /gene="hemC"
                     /locus_tag="VN518_00520"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009466433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HXE03087.1"
                     /translation="MGDRKLLIGTRGSPLALWQANHVRALLIDAHGPGEDAVALEPIT
                     TSGDRIRDKPLRDFGGKGLFTKEIDEALLAKRVDIAVHSMKDLPTDLPPGITIAAVLP
                     RGDVRDAFISAKGESLAALPPGAVVGSSSLRRQAQVKRLRPDLQAIDFRGNVETRLKK
                     LDQGMVDATLLALAGLERLGLTRHVTSVLPTDEMLPAVAQGAIGVSCRSDDVKTRDLL
                     RALNDDRTATAVSCERAFLGELDG"
BASE COUNT         1056 a         1917 c         1918 g         1098 t
ORIGIN      
        1 gccaggcagc ggcgtgatca ggatgcctcc ggtctcggtt tgccaccaag tatcgacgat
       61 cgggcagcgc tcctcgccga ccacgcggta ataccactcc caggcttccg gattgatcgg
      121 ctcgcccacg ctgccgagca cccgcagtga cttccggctg gtgtgcgtga cgtagtcgtc
      181 gccggcgccc atcagcgccc taatcgcggt cggcgcggtg tagatgatgt tgaccccgtg
      241 cttgtccacc acctgccaga agcgtgatgg gtccggatgg ttgggcacgc cttcgaacat
      301 caaggtggtc gctccgttgg cgagcgggcc atagacgata taggagtgac cggtgaccca
      361 gccgacatcg gccgtgcacc aatagacgtc gccgtcgtga tagtcgaaga tcagctgatg
      421 ggtcagggaa gtatagacga gatagccgcc cgtcgtgtgc accacaccct tcggctgtcc
      481 cgtcgatcct gacgtgtaga ggataaagag cggatcctcg gcgcccatct cctccggcgg
      541 gcaatggtcg gagaccttgg cggcaaggtc gtcgtaccag aaatcgcgcc cggcatgcat
      601 ggcgcattgt ccgcccgtgc gcttgatcac aatgaccttc tcatcgccaa gtgacttggt
      661 cagcgcctcg tcggcattgt gcttgagctt gaccttcctg ccgccgcgca atccctcgtc
      721 agcggtaatg acaagcttcg acttgcaatc ggtaatgcgg ccgacaaggg agtccggcga
      781 aaatccgcca aacaccacgg agtgaatggc cccgatccgg gcgcaggcca gcatggcaaa
      841 ggccgcttca gggatcatcg gcagatagat ggtgacgcgg tcgccgcgct tgaccccaac
      901 tgacttgagc acattggcga agatgttcac ctcgcggtag agctcgccat aggtgatgtg
      961 gcgcggaggc tcggcgggat cgtcgggctc ccagatgatg gcaacctgat gctcgcgctt
     1021 cttcaggtgg cggtcgatgc aattggccga gacgttgagc gtgccgtcct cgaaccattt
     1081 gatcgagatg tctggatagg cgaagctcgt gttcttgacg atgctatagg gcttgatcca
     1141 gtcgatccgt tcgccctgct tgccccagaa cttgtccggc ttctccacgc tcttctcgta
     1201 gagcttctcg tactcgtcct tatcgatgaa ggcgtttttc ttccagtcct tctggacctt
     1261 gtagacggtt tcctccgaca tcggcccgct cctctctaac gcatgcgaag cccgctttca
     1321 tatgaagctt gctcgcatta tggacggcag cgaggctggc gacaatcgtg cgccgcaacc
     1381 aagagttcgg tttctaggct ccgaaggcgc caatgatggc gcccatgacg gcgagcacca
     1441 cgagccaatg ggcggcatcg atgactgtca caatgggctt ggtgccgtga aaggcctggt
     1501 tggtggcggt cgtggtcagc acgaagccga cccagacgaa gaagccgctg atgaggcccc
     1561 cccgcatggt gacatcggcc agatggccca tcagccctgc gagcatccag gccatcaaca
     1621 ggcaccctat ggcggcgatg atgaagggaa gaggggttgg cttgcagtct tctttctttt
     1681 tgccgagcgc gtcctgccag accttgccga agattgtgta ccagccccag cccacgagga
     1741 atccggcgat ggcggcgatg acgactgcga ggtagttgat cgaaaatgcc ggcatttgct
     1801 ccccctgttc tccctagcgt gacttctcct taagcccgac gagccggctg atgatactcc
     1861 actcttcggc ggagaccggc tgcaccgaga gcctcgaatt gttgaccagc accatgtcct
     1921 tgagcgcctt gttcgccttg atctcggcaa gcgtcaccgg cctcttcacg tcggacattg
     1981 ccgcgacatc gacgcactcc cagacgcctg ttccatcggt cgagtcggga tgcgcctcgg
     2041 cgaccacctt gaccaccccg acaatgcgct tctcctcgcc tgagtgatag aagaagccga
     2101 ggtcgccctt tttcatcgat cgcatgacgt tgcgagcctg gtagttccgc acgccgtccc
     2161 actcgccgcc cttggcgcct tcgttcttct gctggtccca ggaccaggcg tccggctcgg
     2221 atttgaacag ccaatagttc attgcactag gctcactggc ttgggttgtt gagggtccag
     2281 atccaggggc ggatctcgac cgaggcgaac agccctgctt tggcgtgggg atctccggcg
     2341 gcgatctcct ttgccgcctg cagcgacgcc gcctcgatga cgacgaggct gccgttcatg
     2401 gtttcgccgt cggcctcggt gaacggaccg gcgagtttga gcatgccgcc gaggcctttg
     2461 aggtatgcga catgctcggg acgcttcgcc ttgcggaggg cgagactgtt cggcttgtcg
     2521 gtgcagatca aagcgtagag cattgtggtc cctctttgca gaagaggcta ttcccgagcg
     2581 gggcgagctg tccacacagc tcggctaccc ctcgggcctg aggggcctcg cgagcagggc
     2641 ttcgatcgct tcatcgaccg tggcgaggcc cgagactacg gcgtgcaccg cctgggcgat
     2701 cggcatctca atgccgcgcg cagcgatctc gaccacggcg ctcgcagtat agaccccctc
     2761 ggtcaccgag aggcgcgact tcaacacctc gcctaagctc cgcccttcgc cgagcgcgct
     2821 gccaagcgac atgttgcgcg actgcgggct gccgcaggtc aggatcaaat cgccgagacc
     2881 agacagcccg gtcatggtct cgcgccgggc accaaggctt ccgccaaagc gcatcatctc
     2941 ggcgaagcct cgcgtgacca gcgcggcatg cgcgctcgcg ccaagcttct tgccgacgac
     3001 gatgccggcg gcgatggcgt agacgttctt gagcgccccg ccgatctcgg cgccggcgac
     3061 gtcatcgctc caatagcagc ggaacgaggc atggctcaag gcatgcgcaa gagcgcggcc
     3121 atgcgcttcg ctcgcggtgg caagggtcaa ggcggcggga agtccgcgcg cgatctcggc
     3181 ggcgaagctc ggacccgaga gcacggcggt gcgcacttgc ggcagaacct ctgcgaccac
     3241 ttgggagagc aattgtccgg tcgattgctc gatgcccttg gcgccgatca cgagaggctg
     3301 atcggtgcgc atatgagggg caagctcatc ggcgatggcg cggacctcct gcgccggtgt
     3361 caccatcagc acgaggtcgc aggcggcgat ttcgttgagc ttggcggtgg ccttgatggc
     3421 agggtcgagc gagacccctg ggagataaag cgcgttccga tgctgctggt tgatttcggc
     3481 aatggtctcg ggctcgtagg cccaaagcat cacgtcgcgg ccggcgcggc gcgcgctgac
     3541 cgcaagcgcc gtaccccagg cgcctccccc gacaatgccg acgctctgca gggccatgtg
     3601 accttatcgc tcatgccatg gtggatggga gctgccccct tatgccagat ttcatcatgc
     3661 cagagccaaa ggggaatgat gaaggatgga gaacgaagtc ttggtcgagt gggccaaggc
     3721 cttcgtgcag gccgtcgccg cgcaagcgcc ctgggccgaa atcgagccgt attacgcccc
     3781 tgatgtcagc caagaggagt tttcccaacc ggcttctgcc caagggcacc gttcgcaatc
     3841 tggaagccct gcgcgaggcg agcgccaagg ccgcaggtga tcaaagcgca gaccatcgag
     3901 atcgtgaacg ccgtctcgtc aggccaatgc gtggttctcg aggcgatctg gaccggcacg
     3961 ctcgcggtcg gcttcggcac gctgaaaccc ggcgaccaat tgcgcgcccg cttcgcccaa
     4021 gtcttcgagt tcaaagacgg gttgatctgg cgtcagcgca attatgattg cttcgaggcc
     4081 tggtgatcag gctttcacgc cggcgccgat ggccggcgcc gcgtccggat cgagcggcca
     4141 gcgggcgcgg gacggcgcgg caagatcgtc gacgagccct cggacgagcc gctcggctcc
     4201 ggcccaggcc accatggcgg cgttgtcggt gcaaagttcg gccggcggca ggatgagtgc
     4261 gaagccttgc ttggcggcgg cgtctgcgag ggcagcgcgc aagcgctgat tggcggcaac
     4321 gccgccggcg acgaccaacg ccttttgcgc atcaggccca agcacatcga cgtaaagctc
     4381 catggcgcgg cccgtgcggt cgctgaccac gtcagccgcg gcctcctgga aggaagcgca
     4441 aaggtcggca acgtcttgct cgccgagcgg ctcgcgcttc atcgccgcgt gcctgagcgc
     4501 ggtcttgagc cccgcaagcg agaaatgcgg ctcggagcgc ccgagcatcg gccgcggcaa
     4561 ggcgacggtc gcgcgcccgc gcttggcgca ttcctcgacc gccggcccgc ctggatagcc
     4621 aagcccaagc aattttgcag ccttgtcgaa ggcctcgccc agcgcatcgt ccaaggtcgt
     4681 gccgaggcga gtataggagc ccacaccttt gacgatcagg agctgcgtat gaccgccaga
     4741 gacgagaagc agcagataag ggaagcgcaa gccgtgggtc aggccggtag tgagcgcatg
     4801 cgcctcgaga tggttcacgg cgatgaacgg cagcccgcgc gccaaggcga tggccttggc
     4861 ggtggtgacg ccgacgatca acccgccgat caggccaggg cctgccgtcg cggcgacgcc
     4921 gtcgatctcc tcccagccga gacgagactc ggcgagcgcc tcggcgatga gacgatcgag
     4981 cagctccaca tgggcgcgcg ccgcgatctc cggcaccacg ccgccgaact cggcatgcag
     5041 cgcaagctgc gacctcacca gattggcaag gaggacgcct ttgccgctcg cctcgcggcg
     5101 gaccaaggct gccgcggtct cgtcgcagct cgtctcgatg ccgaggacaa cgaacgcgcg
     5161 cttctctggg acagtagcca tcatgcttct ttacagggta agaccggggc gattgaagag
     5221 tatgatgcct tcctttctgg ttaaggcaat ggagtcaagc acttgggcga tcgcaagctt
     5281 ctcatcggca cgcgcgggag cccgctcgct ttgtggcagg cgaaccatgt gcgcgctctg
     5341 ctgatcgatg cgcatggccc tggtgaggac gctgtcgcgc ttgagccgat caccacctct
     5401 ggcgaccgca tccgcgacaa gccgctccgc gacttcggcg gcaaggggct gttcaccaag
     5461 gagatcgacg aggccctgct cgcaaagagg gtcgatatcg ccgtgcattc catgaaggac
     5521 ctgccaacgg accttccgcc aggcattacc atcgccgccg tgctgccgcg cggcgatgtg
     5581 cgcgatgcct tcatcagtgc caagggtgaa tcgcttgccg cgttgccgcc tggtgcggtg
     5641 gtgggaagct cctcgctccg ccgccaggct caggtgaagc gcctgcggcc tgaccttcag
     5701 gccatcgact tccgcggcaa tgtcgagacg cgccttaaga agctcgatca aggcatggtg
     5761 gacgccaccc tgctcgcgct tgccgggctc gagcggcttg gtctcacccg ccatgtcacc
     5821 tcggttctgc cgacggatga gatgttgccg gctgtggcgc aaggcgccat cggcgtttcc
     5881 tgccgcagcg acgatgtgaa gacgcgtgac ctgttgcggg cgctgaacga tgaccgcact
     5941 gccaccgccg tctcttgcga gcgcgccttc cttggagagc tcgacgggt
//
LOCUS       DATNAE010000029         6040 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3641255, whole genome shotgun sequence.
ACCESSION   DATNAE010000029 DATNAE010000000
VERSION     DATNAE010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6040
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3641255"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            714..1013
                     /locus_tag="VN518_00525"
     CDS             714..1013
                     /locus_tag="VN518_00525"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HXE03088.1"
                     /translation="MDTRGIDLACRLGGEEFVVMMPDCDLAKAYQVGERLRQCIEAAP
                     FEVGGKVGLLQVTASVGVGALELDDGAPEHVLKRAYEALYCAKRDGRNRVVADAA"
     gene            complement(1516..1683)
                     /gene="rpmG"
                     /locus_tag="VN518_00530"
     CDS             complement(1516..1683)
                     /gene="rpmG"
                     /locus_tag="VN518_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011454349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HXE03089.1"
                     /translation="MAKPVTQKIKLVSSAGTGYYYVTKKNPRNQTEKLVFKKYDPVAR
                     KHVDFKESKIK"
     gene            complement(1761..2246)
                     /locus_tag="VN518_00535"
     CDS             complement(1761..2246)
                     /locus_tag="VN518_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HXE03090.1"
                     /translation="MSMQERSVPDSAGVARRDARSTGERSVLTSLLRGARLTCPACGK
                     GAMFRRYLKVADNCPACGEALHHHRADDAPPYFTIVIVGHIVVGLVLAVEMAYRPPLW
                     LHAALWLPLTVLLALLILPPVKGALVGLQWALLMHGFDPNAEEEKIEPFDVGIRNGRG
                     R"
     gene            complement(2318..4597)
                     /gene="rnr"
                     /locus_tag="VN518_00540"
     CDS             complement(2318..4597)
                     /gene="rnr"
                     /locus_tag="VN518_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HXE03091.1"
                     /translation="MAKTKNAPKSQKVGKLPSKQEILDFLQGASVQAGKREIARAFGV
                     KGGDRVALKELLRDMADDGLIAGSRRKLQRPGVLPSVTVLEIVERDSYGEFIARPANW
                     PEEHGPPPRILIIESKQDKGPAAGIGDRVLARITPLGPDADTPYQARPIKRLPRATRS
                     QLGIFRALPGGHGVIDPVDRKHLKEWRVARDATNDAENGELVRFELGGPARFGGANAR
                     VTDRLGNPQDQRATSLIAIHAHGIPDTFPEAVLIEADAAKEPDLKRREDLRHLPFVTL
                     DPVDARDRDDAVWAEADPDPRNKGGWVVMVAIADVASYVKPGSALDKEARARGNSVYF
                     PDRVVPMLPERISNHLCSLQAAEARPCLAVRMVFDKDGRKRSHRFLRGLMRSAAALAY
                     AQAQAAIDGNPDDTTGPLLEQVLLPLWGAYGSLTRARTERAPLDLDLPERKIQLDEQG
                     RVRGVTTPPRLDAHRLIEEFMIAANVAAAEELEAKHTPLIYRVHDQPSKEKLAALSEF
                     LSTLGVKLPKIGQLKPAHFNRILAETRASPAAELVGEMVLRSQAQAEYSAGNFGHFGL
                     NLRRYVHFTSPIRRYADLVVHRALIRALKLGNDGLTDAEIPLLEKVAEAISGTERRAM
                     AAERETADRLIAAFLADRIGARFAARVSGLTRTGLFVRLNETGADGFVPASSIGHEYF
                     YHDEKRQALIGEETGAAFQLGDPVEVRLFEAIPSAGALRFELLSEGRMIGVKPARGRI
                     QGQWRKPQAPRKGRGRGRA"
     gene            complement(4626..>6040)
                     /locus_tag="VN518_00545"
     CDS             complement(4626..>6040)
                     /locus_tag="VN518_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HXE03092.1"
                     /translation="EIERTTAEIGVTGNDGVAYGLRATGSVIRFDGFLKLYEEGTDDV
                     IGEDGALPILAQGDQLKGRKIEAKQHFTEPPPRYSEASLIKKMEELGIGRPSTYASTL
                     NILRDREYVRLDKKRLHPEDKGRLVTAFLENFFRRYVEYDFTADLEEKLDLISAGQIA
                     WKDVLRDFWRDFIAAVHDIGDLRIAEVLDALNDMLGPHIFPGTEAGGDPRACPSCETG
                     RLSLKIGKFGAFIGCSNYPDCRFTRQLADGGDKTGALGSEGKLLGTDPETELDVTLRT
                     GRFGPYVQLGENGEEKPKRASIPKGFDPEGIDVTRALSLLSLPREIGAHPETGKPITA
                     GFGRFGPYVQNDGKYASLSGADEVFTVGINRAVSLLAEKAANRIRRGATVIKDLGEHP
                     ELKGAVQVLSGRYGPYVKHGKINATLPKDRNPEEVTMADAVELIAARAAKGPAKKPAR
                     KASKAKKTKKEKEVVEETAH"
BASE COUNT          992 a         1848 c         2023 g         1177 t
ORIGIN      
        1 cggttgaaga tgctgaccga cgagatgctg atgcgcgctt cgaccgagga acagttgggt
       61 ttcgcgggcg acttgcataa gcccttgcga ccaggttgca cgcggcgggc ggatactgct
      121 cgtcgaggac aatgacagct cctaccggcg cattcaggac gcgctcaggg acgagttcga
      181 gctcgcccgg gaggccgatc ctgcaagtgc gctgctgcgg ctccccgacg gcgatttcga
      241 tctcatcatc gtaagcctca gcctgcggga tgcggacagc ttgcggctat gctcgcaagt
      301 tcgctcgctc gaccgggcac gccatttgcc gctcctgatc atcgtcgagc ctggcgacga
      361 tgcgcctctg ctcaagggtc tggacatggg ggtcaacgac tacgtggtgc gcccgatcga
      421 cccgaacgag ctgctggcga gggtgcgtac ccagctcaag cgcaagcgtt atctcaacca
      481 tttgcgtcgg ctcgaggaga gcgtcgagat ggccattctc gacccgctca ccgggctgca
      541 caatcgccgc tatatggcga gccatctcgc cacgctgttc gatgacgcct cacggcgcgg
      601 caagcctctt tcggtgctgc tgatcgacat cgaccatttc aaggcggtca acgacagtca
      661 tggccacgat gccggggacg cggtgttgcg ggagttcgcc acgaggattc gccgtggaca
      721 cgcgcggcat cgatctcgcc tgccggcttg gcggcgagga gttcgtggtg atgatgccgg
      781 actgcgactt ggccaaggcc tatcaggtcg gcgagcgcct gcgccaatgc atcgaggcgg
      841 ctcctttcga ggtcggagga aaggtcggcc tgttgcaggt cacggcgagc gtcggggtcg
      901 gggcgctcga attggacgac ggcgcgcctg agcacgtgct gaaacgggcg tatgaggcgc
      961 tctattgcgc gaagcgcgat ggccgcaacc gggttgtggc cgacgcagcc tagaggcctc
     1021 cggccaaggc aatcagccga atcacccaca gattttcgtt gctagccttg gtttttcgac
     1081 ttgacgttgc gcttgaagcc ttctggttaa ggcgttacac tggctgcgtt ttcaccagat
     1141 tagccacgca atagagtttg attttttcga tttgtcggct agtttgtatt tgacttgaca
     1201 ccggtaagaa ttgaggccgg aggtaaagta tggggtgagg ggaaatgcgc gtgtggggct
     1261 cgtgctcgcg catctcgaca tcatcaatga gataacccta cggaatgctc aggtccgccg
     1321 catctcctgg gtccgtgggg ttcggtcggc cggagcgcgg tttgagtggc ctcctcgtta
     1381 gcgccgcgtt tcggccgacc acctattgcc aagaacagaa aacttggcgg gtggccgggc
     1441 catcacttcc acgacgtggc gctccttatg ccatctgctg atggacatat ccgccggcga
     1501 cagggctctc gccctttatt tgattttgct ttctttgaag tcgacgtgct tgcgggcaac
     1561 cggatcgtac ttcttgaaca cgagcttctc ggtctggttc cgcgggttct tcttcgtgac
     1621 gtaataatag ccggtgcccg cgctgctcac gagcttgatc ttttgcgtga cgggctttgc
     1681 catttcatcc tcgctctcaa aaaaggttgg gcgaacccta ctcgccagga cccggaagtc
     1741 aagaccggga agtcaagccg tcagcggccc cggccgttgc ggatccctac gtcgaagggc
     1801 tcgatcttct cctcttcggc attgggatcg aagccgtgca tgagcagcgc ccattgcagg
     1861 ccgacaaggg cgcccttgac cggcggcagg attaggagcg caagcaagac cgtgagcggc
     1921 agccagagtg ccgcatgcag ccaaagcggc ggccggtagg ccatctccac ggctagcacc
     1981 aggcccacca cgatatggcc gacaatgacg atggtgaaat agggaggcgc atcatcggcc
     2041 cggtgatggt gcagcgcctc gccgcaagca gggcaattgt cagccacctt gagatagcgg
     2101 cggaacatgg cgcctttgcc gcaagccggg caggtcagcc tcgcacctct cagcagcgac
     2161 gtcagcacgg agcgttcgcc ggtcgatcgc gcgtcgcggc gggcgacgcc ggcgctgtcg
     2221 gggactgatc tttcctgcat gctcattcct tatcgaagtt ccgcaatcct gccaaaccgg
     2281 atcgtggcac gcggcctcat gccgcgctct gttttgctca agcccgcccg cgtccccgcc
     2341 cttttctggg agcttgcggc ttccgccatt gcccctggat gcggccgcgg gcgggcttga
     2401 cgccgatcat ccggccctcg ctcaagagct cgaaacgcag cgccccggcg gatggaatgg
     2461 cctcgaacag gcgcacctcg acgggatcgc cgagctggaa ggcggcgccg gtttcttcgc
     2521 cgatcaaggc ctggcgcttt tcgtcatgat agaaatattc gtggccgatg gaggaggccg
     2581 ggacgaagcc gtcggcgccg gtctcgttca gccgcacgaa gagcccggtg cgggtgaggc
     2641 cggagacgcg ggcggcgaag cgggcgccga tgcgatcggc aagaaaagcg gcgatcagcc
     2701 ggtcggcagt ctcgcgttcc gccgccatgg cgcgccgctc ggttccggag atcgcctcgg
     2761 cgaccttttc gagcagaggt atttcggcgt cggtcaggcc gtcattgccg agcttgaggg
     2821 cgcggatcag cgcccggtgc accacgagat cggcatagcg gcggattggc gaggtgaagt
     2881 gcacgtagcg ccgcaagttc agaccgaaat gaccgaaatt gccggcgctg tactcggcct
     2941 gagcttggct gcgcagcacc atctccccca caagctcggc cgcagggctc gcccgcgtct
     3001 cggcgaggat gcggttgaaa tgggcgggct tcagttgccc gatcttcggc agcttcacgc
     3061 ccaacgtcga gagaaactcc gagagcgcgg cgagcttctc tttcgaaggc tgatcgtgca
     3121 cgcgatagat caagggcgtg tgcttggcct ccaattcttc cgctgcggca acattggcag
     3181 cgatcatgaa ctcctcgatc aggcgatggg cgtcgaggcg gggaggggtg gtgacgccac
     3241 gcacgcggcc ctgctcgtcg agctggatct tgcgctcggg caggtcgagg tcgaggggcg
     3301 cgcgttcggt gcgcgccctg gtgaggctgc cataggcgcc ccatagcggc agaagcacct
     3361 gctcgagcaa cggtccggtc gtgtcgtcgg ggttgccgtc gatcgctgct tgcgcctgcg
     3421 cataggcgag tgccgccgcc gagcgcatca ggccgcgcag gaagcgatga ctgcgtttgc
     3481 gcccatcctt gtcgaacacc atgcgcacgg cgagacaggg ccgcgcctcc gccgcctgca
     3541 gcgagcagag atgattggag atgcgctcgg gcagcatcgg caccacgcgg tccgggaaat
     3601 agacggaatt gccgcgggct ctcgcttcct tgtcgagcgc gcttccgggc ttcacgtagg
     3661 aggcgacgtc ggcgatggcg accatgacca cccaaccgcc tttgttcctg ggatcggggt
     3721 ccgcttcggc ccacaccgcg tcgtcgcggt cgcgggcgtc cacgggatcg agggtgacga
     3781 agggcagatg ccgcagatcc tcgcggcgtt tcaggtcggg ctctttcgcc gcgtcggcct
     3841 cgatcagcac ggcttccggg aaggtatcgg gaatgccgtg ggcatgaatg gcgatgaggc
     3901 tcgtcgcccg ctgatcttgc ggattgccga gcctgtcggt gacgcgggca ttggcgccgc
     3961 cgaagcgggc cgggccgccg agctcgaagc ggacaagctc gccgttctcg gcgtcattag
     4021 tggcgtctct ggcgacgcgc cactctttca gatgcttgcg gtcgacggga tcgatgacac
     4081 cgtggccgcc tggaagggcg cggaagatgc cgagctgact tcgcgtggcg cggggaagcc
     4141 gcttgatcgg ccgcgcctgg tatggggtgt cggcgtcagg gccaagcggc gtgatgcgcg
     4201 ccagaacgcg atcgccgatg ccggcagccg gccccttgtc ctgcttactc tcgataataa
     4261 ggatgcgcgg aggtgggcca tgctcctcag gccagttggc gggcctggcg atgaactcgc
     4321 cgtaggaatc gcgctcgacg atctcgagca cggtcacgga cggcagcacg ccggggcgct
     4381 ggagcttgcg gcgagagccc gcgatcagtc cgtcgtcggc catatcgcgc aacagctctt
     4441 tcagagcgac gcggtcgccg cctttgacgc cgaaggcgcg cgcgatctcg cgcttgccgg
     4501 cttgcacgct ggcaccttgc agaaagtcca gaatctcttg cttggaagga agcttgccca
     4561 ctttttggga tttgggagcg ttcttggtct tggccacgcc tctttttagc acgcgcgggt
     4621 caaaactagt gtgccgtctc ctcgacgacc tctttctctt tcttcgtctt tttcgctttg
     4681 ctcgccttgc gcgcaggctt ctttgccggc cccttggcgg ctcgcgcggc gatcagctcg
     4741 acggcgtcag ccatcgtcac ctcttcaggg ttccggtcct tgggcaaagt ggcgttgatc
     4801 ttgccgtgct tcacgtaagg tccgtagcgg ccgctcaaca cctgtacggc gcctttgagc
     4861 tcggggtgct cgccgaggtc cttgatgacg gtggcgcctc ggcgaatgcg gttcgcggcc
     4921 ttctcggcga gcaggcttac ggcgcgattg atgccgacgg tgaagacctc gtcggcgccg
     4981 ctcaagctcg catatttgcc gtcgttctga acgtagggcc cgaagcggcc aaagccggcg
     5041 gtgatgggct tgcccgtttc gggatgagcg ccaatttcgc gcggcagcga cagcaatgag
     5101 agcgcgcgcg taacatcgat accctccgga tcgaaaccct tggggatgga ggcgcgtttc
     5161 ggcttctcct cgccattctc gccaagctgc acgtaaggcc cgaagcgccc ggtgcgtaac
     5221 gtgacatcga gctcggtctc aggatcggtg ccgagcagct taccttcgct gccgagcgcg
     5281 cccgtcttgt cgccgccgtc ggcgagctgg cgggtgaagc ggcagtcggg atagttggag
     5341 cagccgatga aggcgccgaa cttgccgatc ttgaggctca agcggccggt ctcgcaggag
     5401 gggcaggcgc gcggatcgcc accggcttcc gtgcctggga aaatgtgcgg cccaagcatg
     5461 tcgttcagcg catcgagcac ctcggcgata cgcaagtcgc cgatatcgtg gacggcggcg
     5521 atgaagtcgc gccagaagtc gcgaagcacg tccttccagg cgatctgtcc tgccgagatc
     5581 aggtcgagct tctcctcgag gtcggcggtg aagtcgtatt ccacgtaacg gcggaagaag
     5641 ttctccagga acgcggtgac gaggcggccc ttgtcctcgg gatgaagccg cttcttgtcg
     5701 agccggacat attcgcggtc gcgcaggatg ttgagcgtcg aggcataggt cgacggacgg
     5761 ccgatgccga gctcctccat cttcttgatg aggctcgctt cgctgtagcg gggcggcggc
     5821 tcggtgaaat gctgcttggc ctcgatcttg cggcctttga gttgatcgcc ttgggccaaa
     5881 atggggaggg ctccgtcctc gccgatcaca tcgtcggtgc cttcctcgta gagcttgagg
     5941 aagccgtcga agcggatcac cgagcccgtg gcacgcaagc cgtaggccac gccgtcattg
     6001 ccggtgacgc cgatctcggc tgtggtgcgc tcgatctcgg
//
LOCUS       DATNAE010000030         3345 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_931980, whole genome shotgun sequence.
ACCESSION   DATNAE010000030 DATNAE010000000
VERSION     DATNAE010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_931980"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="VN518_00550"
     CDS             complement(<1..554)
                     /locus_tag="VN518_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016920650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXE03093.1"
                     /translation="MQNGREVLISVRGLAKSFGGYQIWDGLNLDVYRGEILAIVGGSG
                     QGKSVLLRVITGLVKPDMGEVELFGRNAKTLTPQDRVDIESRWGILFQDGALFSSLTV
                     LQNIQVPMLEHLELPQGMMDDLAFLKLAMVGLPPEAAHKYPSELSGGMKKRAGLARAL
                     ALDPEIVFLDEPTAGLDPIGASDF"
     gene            complement(557..1735)
                     /locus_tag="VN518_00555"
     CDS             complement(557..1735)
                     /locus_tag="VN518_00555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXE03094.1"
                     /translation="MLKRDHTLSLSTQARAPGFRVELRGTTFRLIASGAWTVAEATKL
                     DIALKRLRVPIPPTPEFTGEMDIAGIAEIDTAGALLLQRTAASWEAGGLRTRYAGATE
                     SFRILIEEVHKRGSTERPKIKKTGAAQQLTEDVTKALTGVWSDAIQLTSFLGEVTSAF
                     ARMVVQPWRFRPKSFVHHLDHAGLRAVPIIALICFLIGAVVMQQGVVQLKPFGAEPFA
                     VNMVAILALREVGILLTAIMVAGRSGSAFTAEIGSMKMREEIDAMRTLGIDPMDTLVL
                     PRVLALVVALPLLTFLGDITGLIGGGVMAMAVLGLDVSTYIDKLHDAVDVQHFMAGMI
                     KAPFAALIIGLVGCLEGMNVEGSAESLGKHVTSAVVKAIFLVIILDAVFAMFLSAIGM
                     "
     gene            1835..2824
                     /gene="dgcA"
                     /locus_tag="VN518_00560"
     CDS             1835..2824
                     /gene="dgcA"
                     /locus_tag="VN518_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-D-Glu racemase DgcA"
                     /protein_id="HXE03095.1"
                     /translation="MKRARALSVSVERWPIRGGFTISRGSKQEAVVVVATISDGAHQG
                     RGECVPYARYGESVEGVVQAIEVCAPAIGQGLDRVGLQDLLSAGAARNAVDCALWDLE
                     AKRSGRSVAELAGLAPLRAVETAFTISLASPEEMAARARDASRYPLLKLKLGGDGDEV
                     RLVAVRQAVPNARLIGDANEAWRTPKLERLLAAAKAAGIELIEQPLPAGDDALLEHVA
                     RIVPLCADESAHDRASLATIARRYDAVNIKLDKTGGLTEALATAAEARRLGLKIMVGC
                     MVATSLAMAPALLLAQDADFVDLDGPLLLERDRVPGLSYSSGTIMPAPAALWG"
     gene            complement(2776..>3345)
                     /locus_tag="VN518_00565"
     CDS             complement(2776..>3345)
                     /locus_tag="VN518_00565"
                     /inference="COORDINATES: protein motif:HMM:NF015764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXE03096.1"
                     /translation="HALYYTFGTLHWRAQGFADGTIGALWALGVMAEIGLFAVSGPIL
                     AKWGAGRVLFAAGAAAAVRWGLMAFDPPLWATALLQCLHAMSFGAAHLAAIYFLSQAV
                     PEDRGATAQGLYAAVVAGLVLGFATIACGPLYRALGGEAYAVMALLALVGAGSAVFLM
                     RRWRGERVVGPIEVQPQSAAGAGMIVPEL"
BASE COUNT          609 a         1073 c         1143 g          520 t
ORIGIN      
        1 tcgaaatcgc ttgcgccaat ggggtctagg cccgctgtcg gctcgtctag gaacacaatc
       61 tccgggtcta gcgcgagagc gcgcgccaac ccggcacgct tcttcatgcc gccggacaat
      121 tcggaggggt atttgtgcgc ggcctcgggc ggcaggccga ccatggcgag cttgaggaag
      181 gcaaggtcgt ccatcattcc ttgcggtagc tcgagatgct cgagcatcgg cacttggatg
      241 ttctgcagca cggtgagcga tgagaacagc gccccgtcct ggaacaggat gccccagcgg
      301 ctctcgatat cgacgcgatc ctgcggtgtc agagtcttgg cgttgcggcc gaacagctcg
      361 acctcgccca tgtccggctt gaccagcccg gtgatgacgc ggagcagcac cgacttgccc
      421 tggccggagc cgccgacaat ggcgaggatc tcgccgcgat agacgtcgag attgagccca
      481 tcccaaatct gatagccgcc gaagctcttg gcgagcccgc ggacgctgat caagacctct
      541 cggccgtttt gcaaggtcac atcccgatcg ctgacagaaa catggcgaac acggcgtcca
      601 gaattatcac caggaagatc gccttgacca cggcgctggt cacgtgcttg ccgagggatt
      661 cggcgctgcc ctccacgttc atgccttcga ggcagccgac aagcccgatg atcagcgccg
      721 cgaacggcgc cttgatcata ccggccatga aatgctggac gtcgacggcg tcgtgcagtt
      781 tatcgatata ggtggagacg tcgaggccga gcacggccat ggccatcacg ccgccgccga
      841 tcaggccggt gatgtcgccg aggaaagtca agagcggcag cgccacgaca agcgcgagca
      901 cgcgcggcag caccagcgta tccatgggat cgatgccgag cgtgcgcatg gcgtcgatct
      961 cctcgcgcat cttcatcgag ccgatctcgg cggtgaaggc agagcctgag cggccggcaa
     1021 ccatgatggc ggtgagcagg atgccgactt cgcggagcgc aaggatcgcc accatgttga
     1081 cggcgaaggg ctcggcgccg aagggcttca gctgcaccac gccttgctgc atgaccacgg
     1141 cgccgatcaa gaagcagatc agcgcgatga tcggcacggc gcgcaagccg gcgtgatcga
     1201 gatgatgcac gaaggatttg gggcggaagc gccagggctg cacgaccatg cgggcgaagg
     1261 ccgacgtcac ctcgcccaag aagctcgtga gctggatggc gtcggaccac acgccggtga
     1321 gcgccttggt cacgtcctcg gtgagctgct gggcggcgcc cgtcttcttg atcttcggcc
     1381 gctcggtgct gccgcgcttg tgcacctcct cgatgaggat gcggaagctt tccgtggctc
     1441 cggcgtagcg ggtgcgcaat ccacctgcct cccaagacgc agccgttcgc tgcaacagca
     1501 aggcgcccgc cgtatcgatc tcggcgatgc cggcaatgtc catctcgccg gtgaactcgg
     1561 gcgtgggtgg aatcggcacg cgcaagcgct tcagcgctat gtcgagcttg gtggcctcgg
     1621 cgacggtcca ggcgccgctc gcgatcaggc gaaacgtggt gcccctgagc tcgacgcgga
     1681 agcctggcgc gcgcgcttgg gtggagaggc tgagcgtatg gtcgcgtttc agcatgaagc
     1741 ctagcccgaa agacgctgtt tctcggagag ccgcttgttt tgaccgaaaa agccggagaa
     1801 agaaaggcgt tatgctggac ccctggagac gcgcatgaag cgggctagag cgttgagcgt
     1861 gagcgtcgag cgctggccga tccggggcgg attcaccatt tcgcggggct ccaagcagga
     1921 agccgtggtg gtggtggcga cgatcagcga cggcgcccat caggggcgcg gcgaatgcgt
     1981 gccctacgct cgctatggcg agagcgtcga gggcgtggtt caggcgatcg aggtctgcgc
     2041 accggccatc gggcaagggc tcgaccgcgt aggccttcag gacctgctct cggccggcgc
     2101 cgcgcggaat gcggtggatt gcgcgctgtg ggacctcgaa gcgaagaggt cgggacggag
     2161 cgtcgccgag cttgctggtc tcgcgcccct ccgcgcggtc gagacggcct tcaccatctc
     2221 gctcgcctcg cctgaagaga tggcggcgcg ggcgcgagat gcgtcacgct atcctctgct
     2281 caagctcaag cttggcggcg atggtgacga ggtgcggctt gtcgccgtcc gccaagccgt
     2341 ccccaatgcg cggctgatcg gcgatgccaa cgaagcgtgg cgtacgccga agctcgagcg
     2401 cctgctggcg gcggcaaagg cggcaggcat cgagctgatc gagcagccgt tgcctgccgg
     2461 cgatgacgcg ctcttggagc acgttgcccg catcgttccg ctctgcgccg acgagtcggc
     2521 gcatgaccgg gcgtcgcttg caaccatcgc caggcgctac gacgcggtga acatcaagct
     2581 cgacaagacc ggaggcctga cagaggcgct cgccaccgcg gcggaagcgc gaaggctcgg
     2641 gctcaagatc atggtcggat gcatggtggc gacctcgctc gccatggccc ctgccctgct
     2701 gctcgcacaa gacgccgact tcgtcgatct cgacgggccg ctgctgctcg agcgcgaccg
     2761 tgttcccggc ctcagttata gctcaggcac gatcatgccc gcacctgccg cgctctgggg
     2821 ctgaacctcg atggggccga ccacgcgctc gccgcgccaa cgccgcatga gaaagacagc
     2881 gctgccagcg ccgaccaaag cgagcagagc catgacggcg taagcctcgc cgccgagcgc
     2941 gcgatagaga ggcccgcagg caatggtcgc gaacccgaga acgaggccgg cgaccaccgc
     3001 ggcataaagg ccttgagcgg tggcgccgcg gtcctcaggc accgcctggc tcaagaaata
     3061 gatcgcggcg agatgggctg cgccaaagct catggcgtgc aggcactgca atagcgccgt
     3121 cgcccagagc ggcggatcga aggccatcag cccccagcgc accgctgcgg cggcgccggc
     3181 ggcaaacagc accctgccgg cgccccattt ggcgaggatc ggcccggaga cggcaaagag
     3241 accgatctct gccatgacgc cgagcgccca cagcgcgccg atggtgccgt cggcaaaacc
     3301 ttgcgcgcgc caatgcagcg tgccgaaagt gtagtagagg gcgtg
//
LOCUS       DATNAE010000031         3322 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_933600, whole genome shotgun sequence.
ACCESSION   DATNAE010000031 DATNAE010000000
VERSION     DATNAE010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_933600"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /locus_tag="VN518_00570"
     CDS             complement(<1..966)
                     /locus_tag="VN518_00570"
                     /inference="COORDINATES: protein motif:HMM:NF017973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobT"
                     /protein_id="HXE03097.1"
                     /translation="MFLSKRKEAPLDPFKRALTLATRSIAADGTVEVIFSNEPPGLYG
                     KTARLPEPSRVPTRNEIAVIRGHADAAALAVACHDPGLHAKLAPPGGEARAVFEAIEQ
                     ARVEAIGAQRMEGVSANLTARLEQRYERSRINESTDRSEAPLPEALALLVRERLTGLA
                     PPSKARAIANLWRPWIESRARAVLDRMPDTVSDQDAFGRLARDLLAALNLADQLDQTG
                     EEGQEGKEGEPEPSAAEADTEDADGRQQAEFASDAQADQDSLTDETLDEREEGAADRF
                     DRESDMPDAQRLPEPWRPNLSVLDNPEAFGYKVFTRAHDEIVGAEE"
     gene            complement(969..1958)
                     /gene="cobS"
                     /locus_tag="VN518_00575"
     CDS             complement(969..1958)
                     /gene="cobS"
                     /locus_tag="VN518_00575"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobS"
                     /protein_id="HXE03098.1"
                     /translation="MTFAASTHGAGLPDMSVSVRQVFGIDTNLEVPAYSQVDEHVPDV
                     DPDYLFNREVTLAILAGFKHNRRVMIQGYHGTGKSTHIEQVAARLNWPCMRINLDSHV
                     SRIDLVGKDAIVIRDGQQVTEFREGILPYALQSNTALCFDEYDAGRPDVMFVIQRVLE
                     VSGKLTLLDQSKVIRPHPAFRLLSTTNTIGLGDTSGLYHGTQQINQGQMDRWSIVVTL
                     NYLPHDDEVNIVLAKAKSWNTDAGRKIVGNMVRVAELTRNAFMNGDLSTVMSPRTVIT
                     WAENAEIFNDVGTAFALSFLNKCDELERGLIAEFYQRCFGEDLPQSTANVALS"
     gene            complement(2043..2672)
                     /locus_tag="VN518_00580"
     CDS             complement(2043..2672)
                     /locus_tag="VN518_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HXE03099.1"
                     /translation="MKLDSKFFDRLRVKPDAAKLVRDACPTCEWQGCREPGLYPAPKG
                     RGKEGEYHRYCLEHVRQYNKSYNYFAGMPDEDVAQHQKDDTIGHRPTWFVGVNSWARN
                     RATRNGERRNGFAYRFATYDPLAVFGAKAEPAPSTAGEPPRVLRRAERKCLRQLNLED
                     TATKAEVKARFKDLVKRHHPDRNGGDRRSEDKLREVIQAYNYLRQAGLA"
     gene            2855..>3322
                     /locus_tag="VN518_00585"
     CDS             2855..>3322
                     /locus_tag="VN518_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03100.1"
                     /translation="MRARMSWATISGPTMSWATIAALLLAAISVVVTLSLIDYPLGVA
                     WDEVVKLVGVTKGRYAYYHPLFMIDLAQGAALVVQPPDQPSLAHLVRVLGALAGGVMV
                     FATFNLARLVLPDLPALAAAAASAVTPLFVVHARIFKEDIFVAAFLVLALAALI"
BASE COUNT          516 a         1028 c         1140 g          638 t
ORIGIN      
        1 ctcctcggcg ccgacgatct cgtcatgggc gcgggtgaac accttgtagc cgaaggcctc
       61 cgggttatcg agcaccgaga ggttggggcg ccaaggctcc ggcagccgct gcgcgtcggg
      121 catgtccgac tcgcggtcga accggtcggc cgcgccttcc tcacgctcgt cgagggtctc
      181 gtcggtgagt gagtcctggt cggcctgggc gtcggaggcg aactcggcct gctggcgtcc
      241 gtcggcgtct tcggtgtcag cctccgccgc ggagggctcc ggctcgccct ccttcccctc
      301 ctggccttcc tctccagtct ggtcgagctg atcggcaagg ttcaaagcgg caagcaggtc
      361 gcgggcaagg cggccgaagg catcctggtc ggagaccgtg tcgggcatgc gatcgagcac
      421 cgcgcgggcg cggctctcga tccacgggcg ccaaagattg gcgatggcgc gggccttgct
      481 tggcggagcg agcccggtca gccgctcgcg cacgagaagg gcgagagcct ccggcagcgg
      541 cgcctcggaa cggtccgtcg attcgttgat ccggctgcgc tcgtagcgct gctcgagccg
      601 cgcggtgagg ttggccgaga cgccttccat gcgctgggcg ccgatggctt cgacccttgc
      661 ctgctcgatc gcctcgaaca ccgcgcgcgc ttcgccgccc ggcggcgcga gcttggcatg
      721 caggccggga tcgtggcagg cgacggcaag ggccgcggcg tcggcgtggc cgcggatcac
      781 cgcgatctcg ttcctggtcg gcacacgaga aggttcaggc agccgcgcgg tcttgccgta
      841 cagcccaggc ggctcgttcg agaagatcac ctccaccgtg ccgtcggcgg cgattgagcg
      901 ggtggcaagc gtaagcgcgc gcttgaacgg gtcaagcggc gcctctttgc gcttgctcaa
      961 gaacatcgtc agctcaaggc cacgttggcg gtcgactggg gcagatcctc gccgaagcag
     1021 cgctgataga attcggcgat caggccgcgc tcgagctcat cgcatttgtt gaggaaggag
     1081 agcgcaaacg cggtgccgac gtcgttgaag atctcggcgt tctcggccca ggtgatcacc
     1141 gtgcgcgggc tcatcactgt ggacaagtcg ccgttcatga aggcattgcg ggtgagctcg
     1201 gccacgcgca ccatattgcc gacgatcttg cggccggcgt cggtgttcca gctcttggcc
     1261 ttggccagca cgatgttcac ctcgtcgtca tgcggcagat aattgagggt gacgacgatc
     1321 gaccaacggt ccatctgccc ctgattgatc tgctgcgtgc cgtgataaag gccgctcgtg
     1381 tcgccaaggc cgatagtgtt ggtggtcgac aacaggcgga aggccggatg cggcctgatc
     1441 accttggact ggtcgagcaa ggtgagcttg cccgagactt cgagcacccg ctggatcacg
     1501 aacatcacat cgggccgccc ggcgtcgtat tcgtcaaagc agagcgcggt gttggactgt
     1561 agcgcgtaag gcaggatgcc ctcgcggaac tcggtcacct gctgcccgtc gcggatgacg
     1621 atggcgtcct tgccgacgag gtcgatgcgg ctcacatggc tgtcgagatt gatgcgcatg
     1681 cagggccagt tgagccgtgc cgccacctgc tcgatatggg tggatttgcc ggtgccgtga
     1741 tagccctgga tcatcacccg gcggttgtgc ttgaagccgg ccaggatggc gagcgtcacc
     1801 tcgcggttga acaggtaatc cgggtcgaca tccggcacgt gctcgtcgac ctgcgaataa
     1861 gcgggcactt cgagattggt gtcgatgccg aaaacctgac gcaccgaaac tgacatatcc
     1921 ggcaggccgg cgccgtgggt agaagctgca aaggtcatga gatttaagag cctgtcgatc
     1981 gcatgctaga tggggcccgc cttagcgtga gccctgacaa ggcggggtga gggccaagcc
     2041 gctcaagcta gccccgcctg ccgcagataa ttataggctt ggatcacttc gcgcaacttg
     2101 tcctctgagc ggcggtcgcc gccattgcgg tccggatggt ggcgtttgac caggtctttg
     2161 aagcgcgcct tgacctcggc cttggtggcg gtgtcctcga ggttgagctg gcgcaggcat
     2221 ttgcgctcgg ccctgcgcag gacacggggc ggttcgccgg cggtgctcgg cgcaggctcg
     2281 gctttggcgc caaacaccgc gagcgggtca taagtggcaa agcgataggc aaagccgttg
     2341 cggcgctcgc cgttccgggt ggcgcgattg cgcgcccagg aattcacccc cacgaaccag
     2401 gtcgggcggt ggccaatggt gtcgtccttt tggtgctggg ccacgtcctc gtccggcatg
     2461 ccggcgaagt aattgtaaga cttgttatat tggcggacgt gctcaaggca gtatcggtga
     2521 tactcgccct ccttgccgcg gcccttgggc gcggggtaga gacctggctc tctgcaacct
     2581 tgccattcgc aagtcgggca agcgtcgcgc acgagcttgg ccgcgtcggg cttgacgcgg
     2641 aggcggtcga agaatttcga gtcgagtttc attacggcaa ttatgggtag tcgtcctctc
     2701 aagggcaagc gcggcccgct ctctcctacg aaccgcgccg ccaagccggc tcgtcctgag
     2761 gccgaatgcc gctccgattg gagcagccat tgttgaataa agcatgtcag aagcccgtcc
     2821 gggcaggggc agcagagggg agcgggggat aaacatgcgg gcgcgcatgt cttgggcgac
     2881 catctctggt ccgaccatgt cttgggcgac gatcgcggcg cttttgctcg cggcgatctc
     2941 ggtggtggtg acgctcagcc tgatcgacta cccgctcggc gtggcgtggg acgaggtcgt
     3001 caagcttgtc ggcgtcacca agggacgcta cgcctactac catcctctgt tcatgatcga
     3061 tttggcgcaa ggcgcggcac tcgtggtgca gccgcccgat cagccatcct tggctcatct
     3121 cgtccgcgtg cttggcgctt tggccggcgg cgtcatggtg ttcgccacct tcaacctggc
     3181 gcggctcgtg ctccccgacc tgccggcctt ggcggcggcg gcggccagcg cggtgacgcc
     3241 gctattcgtc gtgcatgccc gcatcttcaa ggaggatatt ttcgttgcgg ccttcctcgt
     3301 gctggcgctc gccgcgctga tc
//
LOCUS       DATNAE010000032         4468 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2743091, whole genome shotgun sequence.
ACCESSION   DATNAE010000032 DATNAE010000000
VERSION     DATNAE010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4468
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2743091"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..1472
                     /locus_tag="VN518_00590"
     CDS             195..1472
                     /locus_tag="VN518_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008508646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXE03101.1"
                     /translation="MAIEAKTPNRRGGKSTAAAPSRGSGSEGTVVAVLAAISVCHMLN
                     DVIQSLIMAIYPMLKSALSLDFGQIGIITFTFQGTASLLQPVVGYYTDRYPTPYSLVL
                     GMSSSLIGLLLMAFATNYLMVLAAAALIGMGSSVFHPESSRVARLASGGQHGLAQSVF
                     QVGGNFGTALGPLLAAFVVLPYGQRSLAWFSVVALIAMAMLTFIGGWYGKTIKVNNGK
                     GASVNEMLAISSLGQSRVRRSIAILLALTFSKHFYLVSITSFYIFYLIHTFNLTVQSA
                     QVYLFIFLGAVAAGTVIGGPIGDRIGTRRVIWWSILGVLPFTMMLPYVDLFWTAVLSV
                     IIGVILASAFPAIVVYAQGLIPGRVGMVAGLFFGVAFGIAGIGAAVLGWVADQTSIVF
                     VYHVCAFLPAIGLLAAFLPETAKAKGRRKTGDA"
     gene            1630..2094
                     /locus_tag="VN518_00595"
     CDS             1630..2094
                     /locus_tag="VN518_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03102.1"
                     /translation="MRDIRNDLAERARLIEDQISAAYAQFEKMLEQLQNERDGKVSDL
                     KAEFAALGKLMEVENRRMANVLPLAVQGPAPQLSLADFIMQTLNEIGPMAKDDIVDLA
                     SKEGYFSDAENADRGVHAALVSLHRNEHIRQLQDGTFAPATLAQTVRFRRAM"
     gene            complement(2098..2901)
                     /locus_tag="VN518_00600"
     CDS             complement(2098..2901)
                     /locus_tag="VN518_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HXE03103.1"
                     /translation="MRALRAWLVLSAFFAFTFPLMPLQLLFLWTGSRYARTFPNWYHR
                     QVCRLVGIRLHVDGSVAREHGVLLISNHVSWLDITVLSAVAPVSFVAKQEVASWPFVK
                     WLAKLQRSVFVDRTRRSEVTDKANEILSRLEAGDHVVLFAEGTSSDGNSVVPFKTPLF
                     AAVKPTRGDPLGADLCAQTLALTYTKLHGLPLCRRGRPVVAWYGDMDLASHAWRLLGL
                     GPLDAHIRIGPPVPLDDFTDRKALARYAEDKVRRDVSELLRGRRRPSSI"
     gene            complement(2913..3365)
                     /locus_tag="VN518_00605"
     CDS             complement(2913..3365)
                     /locus_tag="VN518_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HXE03104.1"
                     /translation="MDKTGSRESRIERLCAERGMRMTGQRRVIAKVLSEAEDHPDVEE
                     VYRRASARDPRISLSTVYRTVRLFEGAGILERHEFGDGRARYEPAGHGHHDHLINVKT
                     GEVIEFKNEQIERLQEAVARALGFRLIGHRLELFGTPLKAGTLKDNKS"
     gene            complement(3413..3811)
                     /locus_tag="VN518_00610"
     CDS             complement(3413..3811)
                     /locus_tag="VN518_00610"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXE03105.1"
                     /translation="MAAIHAVCFAKGWDAAEIGQFLGVPGCLALVASTSAAQAPQGFL
                     IVRSAGDEAELLTLAVDPACRRLGLASALLAAATGALRGAGSKRLFLEVDQGNSAARG
                     LYQSLGAVVVGRRPRYYEHGADADIFSLAL"
     gene            complement(3871..>4468)
                     /gene="tsaB"
                     /locus_tag="VN518_00615"
     CDS             complement(3871..>4468)
                     /gene="tsaB"
                     /locus_tag="VN518_00615"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HXE03106.1"
                     /translation="MARGHAEALMPMVAEVMTEAGLDFAALDLIAATLGPGSFTGVRI
                     AIAAARGFALATGAHLWGTDSLAVIAKAALERGMAADGKPIAVAVDARGERLYFGLYD
                     AGGRKLAGPLLIDAGEAAALLPDELAAAVGNGAAHLAEAAMLRGYRLEAKLADLQPNA
                     SALAELALEAEETLPTLRPLYLRPPDAKPQATAAVARR"
BASE COUNT          807 a         1460 c         1440 g          761 t
ORIGIN      
        1 gagcaggatc aaaagctcca ggaccaggac aggccgcatg gcgccctaat gtagctcctg
       61 caaccaagac caagccgcaa acatcatcgg tgcgtgcatc ccgcggccat ccgccacgtt
      121 tccccaaaat gaatccttga agtgcgcgtt gggttgtccc aattctgccg cccgacacac
      181 ctacggcaca caacatggca atcgaggcta agacacccaa ccgtcggggc ggcaagagca
      241 ccgccgcggc tccctcgcgc ggcagcggca gcgaaggcac cgtggtcgcc gtgctcgccg
      301 cgatcagcgt ctgccacatg ctgaacgacg tgatccagtc gctgatcatg gcgatctatc
      361 ccatgctgaa aagcgcgctc tcgcttgatt tcggccagat cggcatcatc accttcacct
      421 ttcagggcac ggcgtcgctg ctgcagccgg tggtcggcta ctacactgac cgctatccga
      481 cgccctattc gctcgtgctc ggcatgtcgt cctcgctcat cgggctcttg ctgatggcgt
      541 ttgccaccaa ctacctgatg gtgctcgcgg cagccgcgct gatcggcatg ggctcctcgg
      601 tgtttcaccc tgaatcttcg cgcgtggcgc gactcgcctc aggcggacag catgggctcg
      661 cccagtcagt gttccaggtc ggcggcaatt tcggcacggc gctcggcccc ctgctcgcgg
      721 ccttcgtcgt gctgccctac gggcagagga gcctcgcctg gttttcggtc gtggcgctca
      781 tcgccatggc gatgctcacc ttcatcggcg gctggtacgg caagaccatc aaggtcaaca
      841 acggcaaggg cgcatcggtc aacgagatgc tcgctatatc gagcctcggg caaagcaggg
      901 tgcggcgttc gatcgccatc ctgctcgcgc tcaccttctc caagcacttc taccttgtca
      961 gcatcacgag cttttacatc ttctacctga tccatacctt caacctcacg gtgcaaagcg
     1021 cgcaggtcta tctcttcatc tttctcggcg ccgtggcggc gggcacggtg atcggcggac
     1081 cgatcggcga ccgtatcggc acgcgccgcg tgatctggtg gtcgatcctc ggcgtgctgc
     1141 ccttcaccat gatgttgccc tatgtcgatc tgttctggac ggcggtgctc agcgtgatca
     1201 tcggcgtgat cctcgcctcg gcgtttccgg ccatcgtcgt ctacgcgcaa gggctaatcc
     1261 ccggacgcgt cggcatggtg gcggggctgt tcttcggcgt cgccttcggc atcgcaggca
     1321 tcggcgccgc cgtgcttggc tgggtcgccg atcagacgtc gatcgtgttc gtctaccacg
     1381 tctgcgcctt tctcccggcg atcgggctgc ttgcggcatt tttgccggag acggcgaagg
     1441 cgaaggggcg gcgaaagacc ggagacgcct gagattccgg caaaaatcag cgtacgtcct
     1501 gttgccctgg gaatcatatg cacaggaact tgcattcgcc ccttccttca gaggcttagg
     1561 ccgtccacag tagggctcgc gagctacgcc acggctcgcg gatttctagc gaaatgcggg
     1621 gagggggcca tgcgtgacat caggaatgat ttggcggaac gagcgagact gatcgaggat
     1681 caaatcagcg ctgcctatgc tcagttcgag aaaatgctcg agcagttgca gaacgagcgc
     1741 gacggcaagg tgtcggatct gaaagccgag tttgccgcgc tcggcaagct catggaggtg
     1801 gagaaccggc ggatggcgaa tgtgctgccg ctcgcggtgc aggggccggc gccgcagctg
     1861 tcgctagccg atttcatcat gcaaacgctg aacgagatcg gtcccatggc gaaggacgac
     1921 atcgtcgacc tggcatcgaa ggaagggtat ttctccgatg ccgagaacgc cgatcgcggc
     1981 gtgcacgccg cccttgtcag ccttcatcgc aacgagcata tccgccagct tcaggacgga
     2041 accttcgcgc cggccacgct ggcgcagacc gtcaggttca ggcgggcgat gtaagcttca
     2101 aattgagctt gggcggcggc ggccgcgcaa cagctccgat acgtcccgcc gcaccttgtc
     2161 ctcggcatag cgggcgagag ccttgcggtc ggtgaagtcg tcgagcggca ccggcggacc
     2221 aatgcggatg tgagcgtcga gcggccccaa gccaaggagc cgccaggcgt ggctcgcgag
     2281 atccatgtcg ccgtaccagg ccacaacggg ccgtccgcgg cggcacagcg gaagcccgtg
     2341 cagcttggtg taggtcagcg ccagggtctg agcgcaaaga tcagccccga gcggatcgcc
     2401 acgtgtcggc ttgaccgcgg caaacagcgg cgtcttgaac ggcaccacgc tattgccgtc
     2461 gctcgaggtg ccttcggcga agagcacgac atgatcgccg gcctcgagcc gggagagaat
     2521 ctcgttggcc ttgtcggtga cctcgctgcg gcgggtgcga tcgacgaaca ccgagcgctg
     2581 caacttggcg agccatttca cgaacggcca cgacgccact tcttgtttgg ccacgaagga
     2641 taccggcgcc acggccgaga gcacggtgat atcgagccag gagacgtgat tggagatgag
     2701 cagcacgccg tgctcgcgcg ccacgctgcc gtcgacatgc aggcggatgc cgacaagccg
     2761 gcagacctga cggtggtacc agttggggaa ggtgcgggcg tagcgcgatc cagtccacag
     2821 gaacagaagc tgcagcggca tcagcggaaa ggtgaaggcg aagaacgccg agagcaccag
     2881 ccaagcccgt aatgcccgca tgatccgccg cgctagctct tattgtcttt cagcgtcccc
     2941 gctttaagcg gggtgccgaa cagctcgagc cgatggccga tcaagcgaaa gccgagcgcg
     3001 cgagccacgg cctcctgcaa gcgctcgatc tgctcgttct tgaactcgat cacctcgccg
     3061 gtcttcacgt tgatgaggtg atcgtgatga ccgtgcccgg caggctcgta gcgcgcgcgg
     3121 ccgtcgccga actcatgacg ctcgaggatg ccggcgccct cgaacaggcg cacggtgcgg
     3181 tacacggtgg agagcgagat gcgcggatcg cgggcggacg cccggcggta aacctcctcg
     3241 acatcggggt gatcttcggc ctcggacagc accttggcga tcacccggcg ctgaccggtc
     3301 atgcgcatcc cccgctcggc gcagaggcgc tcgatccggc tttcccgtga gcctgtcttg
     3361 tccatctaga gggcgctctc tccggacctc ggtcatcatc cgcagtcgag cctcaaaggg
     3421 caagactgaa gatgtcggca tcggcgccgt gctcataata gcgcggacgg cgcccgacga
     3481 cgacagctcc gagagactgg taaaggccgc gcgcggccga attgccctgg tcaacctcga
     3541 gaaacagccg cttcgatccg gcgccgcgca aggctcccgt ggcggcggca aggagcgcgc
     3601 tcgcaaggcc aagccggcgg caagccgggt cgaccgcgag cgtcaacagc tcggcctcgt
     3661 cgccggcgct cctcacgatg agaaaccctt gcggagcttg cgccgccgag gtcgaagcaa
     3721 cgagcgcaag gcatccggga acgccgagaa actgtccgat ctctgccgca tcccagccct
     3781 tggcgaagca gacggcatga atggcggcca tggccgaccc atcggctgca caggccgctc
     3841 gaatttcgat cggaggaccg cttccgcgca tcagcgccgc gccacggcag cggtggcttg
     3901 cggcttcgcg tctggaggcc ggaggtagag gggacgcaag gtcggcaagg tttcctctgc
     3961 ctcaagcgca agctcggcga gcgcagaggc atttggctgc aaatcggcga gcttcgcctc
     4021 caggcgatac ccgcgcaaca tcgcagcttc cgcaagatgt gcggcaccgt tcccgaccgc
     4081 ggcggcaagc tcgtcgggaa gcagggctgc cgcctcgccg gcgtcgatca aaagcggacc
     4141 cgcgagcttg cggccgccgg cgtcatagag cccgaaatag agcctctcgc cgcgcgcatc
     4201 gacggcgacg gcgatgggct tgccgtccgc ggccatgccc ctctccaatg ccgccttggc
     4261 aatcacggcg agcgagtcgg tcccccagag gtgagcgccg gtggcgaggg caaagccgcg
     4321 cgccgcggca atggcgatgc gcacgccggt gaagctccct ggtcccagag tggcggcgat
     4381 aagatcgagc gcggcgaagt cgaggcctgc ctccgtcatc acctcggcca ccatgggcat
     4441 cagggcctcg gcatggccgc gcgccatc
//
LOCUS       DATNAE010000033         2274 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153 k141_31379,
            whole genome shotgun sequence.
ACCESSION   DATNAE010000033 DATNAE010000000
VERSION     DATNAE010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31379"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="VN518_00620"
     CDS             <1..709
                     /locus_tag="VN518_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXE03107.1"
                     /translation="IHIGTTVKELKKNGGDVTATLAAGDKSAQVTAERVVLAVGITGN
                     VENIGLEKTKVRVEKSHIIVNEWLETDEPGVYAIGDVVGPPWLAHKAMHEGVICVERI
                     AGLNTAHPMDVRNIPANTYCRPQVASVGLTERKAKEAGYEIRVGHFPFIGNGKAIAMG
                     EPDGMVKTVFDAKTGELLGAHMIGAEVTELIQGYTIAKTGELTEQELMRTVFPHPTLS
                     EMMHEAVLDAYGQVLHI"
     gene            735..1685
                     /gene="lipA"
                     /locus_tag="VN518_00625"
     CDS             735..1685
                     /gene="lipA"
                     /locus_tag="VN518_00625"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HXE03108.1"
                     /translation="MSEAPLRHPEKAHKPDNPVLRKPDWIRVKAPTSPEYHETRKLMR
                     ALKLNTVCEEAACPNIGECWKQKHATVMILGSVCTRACTFCNVATGRPDQLDPHEPQH
                     VAEAVAKLGLSHIVVTSVDRDDLADGGAAHFVRTIEAIRAAAPVTTIEVLTPDFLKKD
                     GAIEAVVAAKPDIYNHNLETVPRLYAEVRPGARYFASLRLLDRVKELDPAMFTKSGIM
                     VGLGEDKAEVMQVMDDLRAADVDFLTIGQYLQPTPKHHAVHRFVPPDEFAAYRTMAFG
                     KGFLMVSASPLTRSSYFAGDDFQKLRAARAAKLAAAVVKC"
     gene            1833..>2274
                     /locus_tag="VN518_00630"
     CDS             1833..>2274
                     /locus_tag="VN518_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017960303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HXE03109.1"
                     /translation="MPTHAEHRLLPYTPEQLFNLVADVERYPEFLPWCLGARVRERQG
                     NVITADLLIGFKMVRERFSSRVVLDRPQRIDVTYAEGPFRYLNNHWEFIAQPGGQCLI
                     DFYVDFEFRSRMLQKIIGMLFHEAVKRMVGAFETRARQLYGTPAP"
BASE COUNT          423 a          761 c          722 g          368 t
ORIGIN      
        1 gatccacatc ggcaccacgg tgaaggagct gaagaagaac ggcggcgacg tcaccgcgac
       61 gctcgccgcc ggcgacaaga gcgcgcaagt gaccgcggag cgcgtcgtgc tcgccgtcgg
      121 catcaccggc aacgtcgaga atatcggcct cgagaagacc aaggtgcgcg tcgagaagag
      181 ccacatcatc gtcaacgagt ggctagagac cgacgagccc ggcgtctatg ccatcggcga
      241 cgtggtcggg ccgccgtggc tcgcgcacaa ggcgatgcac gagggcgtca tctgcgtcga
      301 gcgcatcgcc ggcctcaaca cggcgcatcc catggacgtg cgcaacatcc ccgccaacac
      361 ctattgccgg ccgcaagtgg cgagcgtcgg attgaccgag cgcaaggcca aggaggctgg
      421 ctacgagatc cgcgtcggcc atttcccctt catcggcaac ggcaaggcga tcgccatggg
      481 cgagcccgat ggcatggtga agacggtgtt cgatgccaag accggcgagc ttttgggtgc
      541 gcatatgatc ggcgccgagg tgaccgagct catccagggc tacaccatcg ccaagaccgg
      601 cgaactgacc gagcaggagc tgatgcgcac ggtattcccg cacccgacgc tgtccgagat
      661 gatgcacgag gccgtgcttg acgcttacgg ccaggttctc cacatctgat tctccggaat
      721 gttgagcagg cccgatgagc gaagctcctc tccgccatcc cgagaaagcg cacaagccgg
      781 acaatcccgt cctgcgcaag ccggattgga tccgcgtcaa ggcgccgacc tcgcccgagt
      841 atcacgagac gcggaagctc atgcgcgcgc tcaagctcaa taccgtgtgc gaggaggcgg
      901 cctgccccaa tatcggcgag tgctggaagc agaagcacgc gacggtgatg atcctgggca
      961 gcgtctgcac gcgcgcctgc accttctgca acgtcgcgac cggccggccc gaccagctcg
     1021 atccgcatga gccgcagcat gtcgccgagg cggtagcgaa gctgggattg agccacatcg
     1081 tcgtgacttc ggtcgatcgc gacgatctcg ccgatggcgg tgctgcgcat ttcgtgcgca
     1141 ccatcgaggc gatccgcgcc gcggcgccgg tgacaaccat cgaagtgctg acgccggatt
     1201 tcctaaaaaa ggatggcgcg atcgaggcgg tggtcgcggc caagcccgac atctacaacc
     1261 acaacctcga gaccgtgccg cggctttacg ccgaggtgcg gccgggcgcg cgctatttcg
     1321 catcgctgcg cctgctcgac cgggtgaagg agctcgatcc cgcgatgttc accaagtcgg
     1381 ggatcatggt tgggctcggc gaggacaagg ccgaggtgat gcaggtgatg gacgatctgc
     1441 gcgccgcgga tgtcgacttc ctcaccatcg ggcaatacct gcagccgacg ccgaagcacc
     1501 acgccgtcca ccgcttcgtc ccgcccgacg agttcgcggc ctatcgcacc atggccttcg
     1561 gcaaaggctt cctcatggtc tcggcctcgc cgctgacgcg ctcctcctat ttcgccggcg
     1621 acgatttcca gaagctgcgc gccgcgcgcg cggccaagct cgcggcggcg gtagttaaat
     1681 gctgatgatc ggcacccgcg gcacccccca ccccaaccct ccccctcaag gggggagggg
     1741 gttttcctgg ttgccagaga ctttcccctc cccccttgag ggggagggaa gggagggggg
     1801 cgacatggcg aaggattggc agatctagtc cgatgccgac ccatgccgag catcggctgc
     1861 tcccctatac gccagagcag ctcttcaatc tcgtcgccga tgtcgagcgc tatcccgagt
     1921 tcctgccgtg gtgcctgggc gcgcgcgtgc gcgagcgcca gggaaacgtc atcaccgcgg
     1981 atctgctgat cggcttcaag atggtgcgcg agcgattctc ctcgcgcgtc gtgctcgacc
     2041 ggccgcagcg catcgacgtg acctatgccg aagggccgtt ccgctatctc aacaaccact
     2101 gggaattcat cgcgcagccc ggcgggcaat gcctcatcga cttttacgtc gatttcgaat
     2161 tccgctcgcg catgctgcag aagatcatcg gcatgctctt ccacgaagcg gtgaagcgca
     2221 tggtcggcgc cttcgagacg cgcgcccgcc agctctacgg cacgcccgct cccg
//
LOCUS       DATNAE010000034         4385 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3944449, whole genome shotgun sequence.
ACCESSION   DATNAE010000034 DATNAE010000000
VERSION     DATNAE010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4385
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3944449"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="VN518_00635"
     CDS             <1..476
                     /locus_tag="VN518_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018045794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HXE03110.1"
                     /translation="GIMPGNIFKAGKVGIVSRSGTLTYEAVAQTTAVGLGQSTCIGIG
                     GDPVNGTNFVDALRLFLADDETEAIIMIGEIGGSAEEEAAAFLAEAKVKKPVVGFIAG
                     VSAPPGRRMGHAGAVISGGKGDAKHKIEAMRAAGIVMADSPGGLGTTLARVLHGG"
     gene            549..3413
                     /locus_tag="VN518_00640"
     CDS             549..3413
                     /locus_tag="VN518_00640"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015315014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HXE03111.1"
                     /translation="MTRHELNDVFATTSFLQGANATYLEQLHASYQQAPDSVGPEWQA
                     FFASLRDDKDDVLLEARGPSWKPSNGLEATVLAPAEFTEEQALAAAQDTLRARMLIRN
                     FRTRGHLLAKLDPLDLSTRKEHPELRPRTYGFTKADYDRQIYLGGALGLEFGTLRQVL
                     DILKRSYCEHIGTEYMHISDPEQRAWIQERIELAENAVRFTPQGKKAILKKLIEAEIF
                     ERFLDVKYTGTKRFGLDGGESVVPALEQVIKRGGQLGLKEIVIGMPHRGRLNVLATVM
                     AKPYRAIFNEFKGGSAHPDEVEGSGDVKYHLGASSDREFDTNRVHLSLTANPSHLEIV
                     DPVVLGKVRAKQDQLHDRERREVLPLLLHGDAAFAGQGVVAECFGLSGLRGHRTGGSL
                     HFIVNNQIGFTTAPRFARSSPYPSDVAKMIDAPIFHVNGDDPEAVVYVTKIAVEYRQR
                     FGKPVVIDMFCYRRHGHNEADEPAFTQPLMYARIGEHPRVTEIYGKKLVEEGLLTSAQ
                     VEGMHTQFRALLEEEFAAGDSYRPNRADWLDGKWSGIGHAEEGARRGVTGVDLERLKE
                     IGRKLTTIPKGFTPHKTIARIMANRRKMIEEGSGIDWATAESLAFGTLLTEGFRVRLS
                     GQDSERGTFSQRHSVLMDQENEKKYTPLKHITKTQGVFEVINSMLSEEAVLGFEYGYS
                     LAEPNALVLWEAQFGDFANGAQVVIDQFISSGERKWLRMSGLVMLLPHGYEGQGPEHS
                     SARLERYLQLCAEDNWQIANCTTPANYFHILRRQLHRQFRKPLVLMTPKSLLRHKRVV
                     STLSQFGPGTSFHRVLWDDAQFLEGQAIKLKPDAEIKRVVLCSGKVYYDLYEEREKRG
                     VDDVYLLRVEQLYPFPARALIQELSRFSEAEMVWCQEEPKNMGAWSFIEPNLEWVLDH
                     IQARHRRPRYAGRPASASTAAGIARKHQLELKTLLEEALG"
     gene            3445..>4385
                     /locus_tag="VN518_00645"
     CDS             3445..>4385
                     /locus_tag="VN518_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018497681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HXE03112.1"
                     /translation="MATEIRVPTLGESVTEATVGQWFKQAGDAVTADEPLVELETDKV
                     TVEVPAPASGVLSEIVVKAGDTVAVGSVLGAIADGDGKVKPAPVKAAAATPQPAPAPV
                     KPSEPPKPAKMQPAALEREPSPREEVSDGGNGNGNGAGTATAEMPPSPAARKYLAETG
                     LSPASVVGSGRRGQVLKQDVLAQAGAAPSGAVATAAASLLAREPKPKAPPAPRPPITP
                     QDETREERVRMTRLRQTIAKRLKEAQDTAAMLTTFNDVDMSAVMQLRREYREAFEKKH
                     GVKLGLMGLFVKACVQALKDIPAVNAEIDGTDIIYKN"
BASE COUNT          844 a         1404 c         1430 g          707 t
ORIGIN      
        1 tcgggatcat gccgggcaat atcttcaagg cgggcaaggt cggcatcgtc tcgcgctcgg
       61 gcacgctcac ctatgaggcg gtggcgcaga ccaccgccgt cgggctcggc caatcgactt
      121 gcatcggcat cggcggcgat cccgtcaacg gcaccaattt cgtcgatgcg ctgcgcctgt
      181 tcctcgccga tgacgagacc gaggcgatca tcatgatcgg cgagatcggc ggcagcgccg
      241 aggaagaggc cgcagccttc ctggccgagg ccaaggtcaa gaagccggtg gtgggcttta
      301 tcgccggggt cagcgcgcct cccgggcgcc gcatgggaca tgccggggcg gtgatctcgg
      361 gcggcaaagg cgacgccaag cacaaaattg aggcaatgcg tgctgcggga attgtcatgg
      421 ccgactcccc gggtggcctt ggaaccacac tcgcccgcgt attacatggg ggttaagccg
      481 gacggcccaa gacttgcctt ttttgaccgg caggccggta gcaccgaacc accggcaagg
      541 ttgatcccat gaccaggcac gaactcaacg acgtgtttgc caccacgtcg tttctgcaag
      601 gcgccaacgc cacctatctc gagcagctgc atgcgagcta ccagcaggcg cccgattcgg
      661 tcgggcccga atggcaggcg tttttcgcga gcctcaggga cgacaaggac gacgtgctgt
      721 tagaggcgcg ggggccgtcc tggaagcctt caaatgggct cgaggcgacg gtgctggcgc
      781 cggcggaatt caccgaagag caggcgcttg ccgcggcgca ggatacgttg cgcgcccgca
      841 tgctgatccg caacttccgc acccgcggcc atctcctcgc caagctcgat ccgctcgatc
      901 tgtctacgcg caaggagcat cctgagctca ggccgcgaac ctacggcttc accaaggccg
      961 attacgaccg ccagatctat ctcggcgggg cgctagggct cgagttcggc acgctccgcc
     1021 aggtgctcga catcctgaag cgcagttatt gtgagcatat cggcaccgag tacatgcata
     1081 tctccgatcc cgagcaacgt gcctggattc aggagcgcat cgagctcgcc gagaatgccg
     1141 tccgcttcac ccctcaaggc aagaaggcga tcctcaagaa gctaatcgag gccgagatct
     1201 tcgagcgctt tctcgacgtg aaatacaccg gcacgaaaag gttcgggctc gacggcggcg
     1261 agtcggtggt gccggcgctc gagcaggtga tcaagcgcgg cggtcagctc gggctgaagg
     1321 agatcgtcat cggcatgccg catcgcggcc gcctcaacgt gctcgccact gtgatggcga
     1381 agccctaccg ggcgatcttc aacgaattca aaggcggctc ggctcatcca gacgaggtcg
     1441 aaggctcagg cgacgtgaaa tatcacctcg gcgcctcgtc tgaccgcgag ttcgacacga
     1501 accgcgtgca tttgtcgctc accgccaatc cgtcgcatct cgagatcgtc gatcccgtcg
     1561 tgctcggcaa ggtgcgggca aagcaggatc agctgcacga tcgcgagcgg cgggaagtgc
     1621 tgccgctctt gctacacggc gatgccgcct tcgccgggca gggcgtggtg gcggaatgct
     1681 tcgggctgtc aggcttgcgc ggccaccgca ccggcggctc gctgcacttc atcgtcaaca
     1741 atcagatcgg cttcaccacg gcgccgcgct tcgcccgctc ttcgccctat ccttccgacg
     1801 tggccaagat gatcgacgcg ccgatctttc atgtgaacgg cgacgatccc gaggccgtgg
     1861 tctacgtgac caagatcgcc gtcgagtacc gccagcgctt cggcaagcct gtggtcatcg
     1921 acatgttctg ctaccgccgc cacggccata acgaggccga cgagccggcc ttcacacagc
     1981 ctttgatgta tgcccgcatc ggcgagcatc ctcgcgtcac cgagatctac ggcaagaagc
     2041 tggtcgagga gggtctgctc acctcggcgc aagtcgaggg catgcacacc cagttccgcg
     2101 ccctgctcga ggaagagttt gccgccggcg actcctatcg cccgaaccgg gccgactggc
     2161 tcgacggaaa atggtccggc atcggccatg ccgaagaggg cgcgcggcgc ggcgtgaccg
     2221 gcgtcgacct cgaaaggttg aaggaaatcg gccgcaagct caccaccatc ccgaaaggct
     2281 tcacgccgca caagacgatc gcgcgcatca tggccaaccg ccgcaagatg atcgaggagg
     2341 gcagcggcat cgattgggcg accgccgaat cgctcgcttt cggcacgctg ctgaccgagg
     2401 gtttccgcgt gcgactgtcg gggcaggaca gcgagcgcgg caccttctcc cagcgtcact
     2461 cggtgctgat ggatcaggag aacgagaaga agtacacccc gctcaagcac atcaccaaga
     2521 cgcaaggcgt cttcgaggtg atcaattcca tgctgtcgga ggaggcggtg ctcggcttcg
     2581 aatatggcta tagcctcgcc gagccgaacg ctctggtctt gtgggaagcg cagttcggcg
     2641 atttcgccaa cggcgcgcag gtggtcatcg accagttcat ctcgtcgggc gagcgcaaat
     2701 ggctgcgcat gtctggcctc gtgatgctgc tgccgcatgg ttatgagggc caggggcccg
     2761 agcattcctc ggcgagactc gagcgctatc tccagctttg cgccgaggac aattggcaaa
     2821 tcgccaattg caccacgccc gccaactact tccatatcct gcggcgccag ctgcaccgcc
     2881 agttccgcaa gccgctcgtg ctgatgacgc cgaagtcgct gctccgtcac aagcgagtgg
     2941 tatcgaccct gtcacaattc ggtccgggga cgagctttca ccgggtgttg tgggacgatg
     3001 cccagttcct cgaaggccag gcgatcaagc tcaagcccga cgccgagatc aagcgcgtgg
     3061 tgctctgctc cggcaaggtc tattacgatc tctacgagga gcgcgagaag cgcggggtgg
     3121 acgacgtcta tctcttgcgc gtcgagcagc tctacccgtt cccggcgcgg gcgctgatcc
     3181 aggaactgtc gcgattctcc gaggccgaga tggtgtggtg ccaggaagag ccgaagaaca
     3241 tgggagcgtg gagcttcatc gagccaaatc tcgaatgggt gctcgaccat atccaggcga
     3301 ggcacaggcg gccgcgctat gccggacgcc cggcctcggc gtcgacggcg gctgggattg
     3361 cccgcaagca ccaattggaa ttgaagacgc tgctcgaaga ggcgttgggc taggcgcggg
     3421 cgattgggga gaggacgaca ggtcatggcc acagagatcc gcgttccgac cctgggcgaa
     3481 tccgtgaccg aggcgacggt gggccagtgg ttcaagcagg ccggcgacgc ggtcaccgcc
     3541 gatgagcctc tggtcgagct tgaaaccgac aaggtgacgg tcgaagtgcc ggcgcccgcc
     3601 tcgggcgtgc tttcagagat tgtcgtcaag gccggcgaca ccgttgccgt cggatcggtg
     3661 ctcggcgcca ttgccgacgg cgacggcaag gtgaagcccg cgccggtcaa ggccgcagcg
     3721 gcgacaccgc aacctgcccc ggctccggtg aaaccgtcag agccacccaa gccagcaaag
     3781 atgcaaccag ccgcgctcga acgcgagccc tcgccgcggg aagaggtgag cgacggcggc
     3841 aacggcaatg gcaacggcgc cggtacggcc acagccgaaa tgccgccgtc gcccgcggcg
     3901 cgcaaatatc tcgccgagac cgggctttca ccggcaagcg tcgtgggctc aggccgccgc
     3961 gggcaagttc tgaagcaaga cgtgctggcg caagccggtg ccgcgccgtc gggagcggtt
     4021 gccaccgcgg cagcttcgct gctcgcgcgc gagccgaagc ccaaggcgcc tccggcgccc
     4081 cgaccgccga ttacaccgca ggacgagacc cgcgaggagc gggtgcgcat gacgcggctg
     4141 cgtcagacca tcgccaagcg gctgaaggag gcgcaagaca ccgcagccat gctcaccacg
     4201 ttcaacgacg tggacatgag cgcggtgatg cagctcaggc gggaataccg cgaggccttc
     4261 gagaagaaac acggggtgaa gctcgggctg atgggcttgt tcgtgaaggc ctgcgtgcag
     4321 gcgctgaagg atatccccgc ggtcaatgcc gagatcgacg gcaccgacat catctacaag
     4381 aacta
//
LOCUS       DATNAE010000035         2521 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3946595, whole genome shotgun sequence.
ACCESSION   DATNAE010000035 DATNAE010000000
VERSION     DATNAE010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3946595"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VN518_00650"
     CDS             <1..208
                     /locus_tag="VN518_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019995183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HXE03113.1"
                     /translation="IEKMVEGRLRKFYEEVVLLAQAFVHDPDTTVAKAVAAAEKTAGA
                     PIKITGFYRFALGEGIDRSSEEAA"
     gene            288..1034
                     /gene="pyrH"
                     /locus_tag="VN518_00655"
     CDS             288..1034
                     /gene="pyrH"
                     /locus_tag="VN518_00655"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HXE03114.1"
                     /translation="MAGTAAAKNLRFRRILLKLSGEALAGDKGYGIDLDVIAPIARDV
                     AAAAAAGAQVCLVVGGGNIFRGLMAAEVGIDRAHADYMGMLATVMNALALQAAIEREG
                     RPARVLSAIPMPTVCEVYIRDKALDHLDKGRVVICAGGTGNPFFTTDTAAALRAAEMG
                     CDALLKATQVDGVYSADPETTPNAKRYDRLSYDDVLKQDLRIMDGAAIALARDNRIPI
                     IVFSIKSQGSLALVLQGKGSSTTIDGKRGA"
     gene            1115..1672
                     /gene="frr"
                     /locus_tag="VN518_00660"
     CDS             1115..1672
                     /gene="frr"
                     /locus_tag="VN518_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HXE03115.1"
                     /translation="MSSFDLKEIERRMRGAFTVLKGEFGGLRTGRANASLLDPIMVMA
                     YGNPMALNQLATINVPESRLITVQVWDRSQVGAVERAIRESDLGLNPVVEGQLLRLPI
                     PELNQERRQEIVKVAHKYAEHARIAVRNVRRDGMEHLKRAEKDGDMGKDEHHALSTKV
                     QDLTDKIIKEIDEALASKEAEIMQV"
     gene            1734..2480
                     /locus_tag="VN518_00665"
     CDS             1734..2480
                     /locus_tag="VN518_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HXE03116.1"
                     /translation="MDELAFNATDRPVACHIAIIMDGNGRWAAERGLPRVEGHRQGVE
                     SVRRTVNAAQDLGITHLTLFSFSSENWSRPRQEINDLFGLLRRFIRRDLADLHKNGVK
                     IRVIGTRAGLERDLLRLIDDAIELTKDNTALNLTIAFNYGARDEIARAARRIAEDVAK
                     GVLAPAEVTQERFATYLDTADLPYPDLLIRTSGELRLSNFLLWQVAYSEFVFVDCYWP
                     DFSREHLEAALAEYQRRNRRFGGTSARSTA"
BASE COUNT          492 a          797 c          810 g          422 t
ORIGIN      
        1 catcgagaag atggtcgagg ggcgcttgcg caagttctac gaggaggtcg tgctgctcgc
       61 gcaggccttc gtgcacgacc ccgacacgac cgtggcaaag gccgttgccg ccgccgagaa
      121 gacggccggc gcgccgatca agatcaccgg cttctatcgc ttcgcgctcg gcgagggtat
      181 cgaccgctcg agcgaggagg cggcgtagcg atcactttgc gacggcttcg gcggctatta
      241 tgcctgagga gagccgacga cgttcggcga gcggggagga gaggctgatg gcagggacgg
      301 cagcggccaa gaacctccgc tttcggcgca tcctccttaa gctgtcaggt gaggcgctcg
      361 ccggcgacaa gggctacggc atcgatctcg acgtgatcgc gcccatcgcc cgtgacgtcg
      421 ccgctgccgc cgctgctggc gcccaggtct gtctcgtggt cggcggcggg aacatctttc
      481 gcgggctgat ggcggcagag gtcggcatag atcgtgccca tgccgactac atgggaatgc
      541 tggcaaccgt gatgaacgcg cttgcgcttc aggcggcgat cgaacgtgag ggacggccgg
      601 cgcgcgtgct ctcggcgatc cccatgccca cggtctgcga ggtctatatc cgcgacaagg
      661 cgctcgatca tctcgacaag ggcagggtgg tgatctgcgc cggtggcacc ggcaacccct
      721 tcttcaccac cgatacggca gccgcactcc gcgccgccga gatgggctgc gatgccctgc
      781 ttaaggcgac ccaggtcgat ggcgtctaca gcgccgaccc cgaaaccacg ccaaatgcca
      841 agcgctacga ccggctcagc tatgacgacg tccttaaaca agatttgcgc atcatggacg
      901 gcgccgcaat cgcccttgcc cgggacaatc gaattccgat aattgtgttt tccatcaaga
      961 gccaagggag cttggctctt gtgctgcaag gaaagggttc ctcgaccacc atcgacggga
     1021 aacgaggagc ttgaggatac gcttacgagc gtacggagct catccctgac ctcgacccga
     1081 tcgatgggcg gggcgggaca gcagggaagg cgatatgtct agcttcgacc ttaaggagat
     1141 cgagcggcga atgcgcggcg ccttcaccgt gctcaagggc gagttcggcg gcttgcgcac
     1201 gggccgcgcc aatgcgagcc tgctcgaccc gatcatggtc atggcctacg gcaatcccat
     1261 ggcgctcaat cagctcgcca ccattaacgt tcctgagtcg cgattgatca cggtgcaggt
     1321 ctgggaccgg agccaggtcg gcgcggtgga gcgcgcaatt agagagagcg acctcggact
     1381 gaacccggtg gtcgagggcc agcttttgcg cctgccgatc cctgagctca accaggagcg
     1441 ccgacaagag atcgtcaagg tcgctcacaa atatgcggag cacgcgcgca tcgcggtccg
     1501 caatgtccgc cgcgacggca tggagcatct gaagcgggcg gagaaggacg gcgacatggg
     1561 taaggacgag caccacgcgc tctcgaccaa agtgcaggac ttgaccgaca agatcatcaa
     1621 ggaaatcgac gaggcccttg cgagcaagga agccgagatc atgcaggtct aatcgggtaa
     1681 ggcgatcgga catcacctaa ccccaaccgg agcagccgac cgtgacgctg gcgatggatg
     1741 agcttgcctt caacgcgact gacaggcccg ttgcctgcca tatcgcgatc attatggacg
     1801 gcaatgggcg gtgggccgcc gagcgcggct tgccgcgcgt cgaaggtcac cgtcagggcg
     1861 tcgaaagcgt cagacgcaca gtcaatgcgg cgcaggacct cggtatcacg catctcactc
     1921 tgttcagctt ctcctcggag aactggtcgc ggccgcggca ggagatcaac gacctgttcg
     1981 ggctgctccg ccgcttcatt cgccgcgatc tcgccgacct acacaagaac ggcgtgaaga
     2041 tccgcgtgat cggcacgcgg gcggggctcg agcgcgatct gctgaggttg atcgacgacg
     2101 ccatcgagct caccaaggac aacaccgcgc tcaatctcac cattgccttc aactacggtg
     2161 cccgtgacga gatcgcccgc gccgcgcgcc gcatcgccga agacgtggcc aagggtgtgc
     2221 tcgctcccgc cgaggtgacg caagagcgct tcgccactta tctcgacacg gctgatctgc
     2281 cctatcctga ccttctcatc cgcacgagcg gtgagctccg cctgtccaac ttcctgctct
     2341 ggcaggttgc ctatagcgag ttcgtgttcg tcgactgcta ctggcccgac ttctcgcgcg
     2401 agcacctcga ggctgcgctc gccgaatatc agcggcgcaa ccggcgcttc ggcggaacct
     2461 cggcgagatc gaccgcgtga cgcgggtaaa atcggcttcc gacaacagca gcgagggcga
     2521 a
//
LOCUS       DATNAE010000036         2043 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_1543168, whole genome shotgun sequence.
ACCESSION   DATNAE010000036 DATNAE010000000
VERSION     DATNAE010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1543168"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1135
                     /locus_tag="VN518_00670"
     CDS             <1..1135
                     /locus_tag="VN518_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HXE03117.1"
                     /translation="RGHPPLSADVPDDSGSMLVRLSVPPASDEEKAKLAAHRVNSLPE
                     PTYGGQLQNRSIQGLNAEGKLKIEYAEQQVKLADGESVSLRAPHYSLTDLGYGPISPD
                     VMISPRVAPPMIGLGLLEAVPEEQILALAQSQAASHDGISGKPNQVWSREHDKVMLGR
                     FGWKAGIPTIAQQAAEAFNGDIGISSTMMPAGAGDCTQKQPDCLNAPNGNSPNYQNVE
                     VGDELFKLVQFYSQNLAVPARRKPDDAQVLHGKAIFYQSGCASCHNPKFVTGEVEGQP
                     HLSHQLIWPYTDMLLHDMGEGLADNRPEGEASGSQWRTPPLWGVGLTETVSGHTLFLH
                     DGRARNVTEAILWHGGEAEPARNKFAALSQTDRDALLAFVNSL"
     gene            1249..>2043
                     /locus_tag="VN518_00675"
     CDS             1249..>2043
                     /locus_tag="VN518_00675"
                     /inference="COORDINATES: protein motif:HMM:NF020932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HXE03118.1"
                     /translation="MRPQISLALVIGSILLCGSGSTAHAEADHAAIARASLTQVIRPG
                     YAAFAVAAGALQGKVATLCQQPSAAALREARDAFAGTVAAWSKVEIIRFGPISQEHRF
                     ERLFFWPDPKGLGLKQIQDALAKQEVTVTEPDEIAGKSVALQGLPALEYLLYGDDGNL
                     PKEDRDFRCRFGLAVATNVDRIAKSVVEGWREGSAYEKAFLGPAPADPYYHAPKEVTL
                     DLFKAFATGIELVRDQKLGKPLGANPAEAKPKLAAFWRSGLTFANAA"
BASE COUNT          375 a          689 c          650 g          329 t
ORIGIN      
        1 ccgcggccat ccgcccctct cggccgacgt gccggacgat tccggctcga tgctggtcag
       61 attgtcggtg ccgcctgcaa gcgacgagga gaaggcaaag ctcgcggcgc atcgggtgaa
      121 ctccctgcca gagccgactt atggcggcca gctccagaac cggtcgatcc agggcctcaa
      181 cgccgaaggc aagctgaaga tcgagtacgc ggagcagcag gtgaagctgg ctgacggcga
      241 gagcgtctcg ctcagggcgc cgcattattc gttgaccgac ctcggctacg gcccgatcag
      301 ccccgatgtc atgatctctc cgcgcgtcgc gccgccgatg atcgggcttg gtctgctcga
      361 ggccgtgccg gaggaacaga ttctcgctct cgctcagtcg caggccgcga gccatgacgg
      421 catctcgggc aagccgaacc aggtctggtc gcgcgagcat gacaaggtca tgctcggccg
      481 cttcggctgg aaagccggca ttccgaccat tgcccagcag gcggccgagg ccttcaacgg
      541 cgatatcggc atctcctcga ccatgatgcc ggccggcgcg ggcgattgca cgcagaagca
      601 gcccgactgc ctcaacgccc cgaacggcaa ctcgccgaac tatcagaacg tcgaggtggg
      661 cgatgagctg ttcaagttgg ttcagttcta ttcgcagaac ctcgccgtgc ccgcgcggcg
      721 gaagccagac gacgcgcaag tgctgcacgg caaggcgatc ttttaccaaa gcggctgcgc
      781 aagctgccac aatccgaaat tcgtcaccgg cgaggtggaa ggccagccgc atttgtcgca
      841 tcagttgatc tggccctaca ccgatatgct actgcacgac atgggcgaag gacttgcaga
      901 caatcgcccc gagggcgaag cgagcggcag ccaatggcgc acgccgccgc tctggggcgt
      961 gggactgacc gaaacggtga gcgggcacac tctgtttctg catgacggtc gcgcgcgcaa
     1021 cgtcaccgag gcgatccttt ggcatggcgg cgaggccgag cccgcgcgca ataaattcgc
     1081 cgcattgtcc cagaccgatc gcgatgcgct gctcgccttc gtcaactcgc tatgaggatg
     1141 accatgggtc agtggcctca cccttcgaga cgggcgctga agcgccctcc tcagggtgag
     1201 ggccatcctc ctcatcctga ggagaggccg caggccgcgt ctcgaaggat gaggccccag
     1261 atcagcctcg cccttgtcat tgggtcgatt cttctctgcg gcagcggatc caccgcgcac
     1321 gccgaagcgg accatgcggc gatcgcgcgc gcctcgctca cccaagtgat ccgccccggc
     1381 tacgccgcgt tcgccgtcgc cgccggcgcg ctccaaggaa aagttgcgac gctctgccag
     1441 cagccttcgg cggcggcgct gagggaagcc agggatgcct tcgccggaac ggtcgcggcg
     1501 tggagcaagg tggagatcat ccgcttcggc cccatctcgc aagagcatcg tttcgagcgc
     1561 ctgttctttt ggcccgatcc caagggcctc ggcctcaagc agatccagga cgccctggcc
     1621 aagcaggaag tgacggtgac cgagcccgac gagatcgccg gcaagagcgt ggcgctccag
     1681 gggttgcccg ccctcgaata tctgctctac ggcgacgacg gcaaccttcc caaggaggat
     1741 cgcgacttcc gctgtcgctt cggcctcgcc gtcgccacca atgtggatcg catcgccaag
     1801 tcggtggtcg agggatggcg cgagggctcg gcctacgaga aggcgttcct cggcccggcg
     1861 cctgccgacc cctattatca cgcgccgaaa gaagtgacgc tcgacctgtt caaggccttc
     1921 gccaccggca tcgagctggt gcgcgaccag aaactcggca agccgctcgg agcgaatccc
     1981 gccgaggcca aaccgaagct tgccgcattt tggcggagcg gactcacctt cgccaacgcc
     2041 gcc
//
LOCUS       DATNAE010000037         2531 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_1843762, whole genome shotgun sequence.
ACCESSION   DATNAE010000037 DATNAE010000000
VERSION     DATNAE010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1843762"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2156
                     /gene="glyS"
                     /locus_tag="VN518_00680"
     CDS             <1..2156
                     /gene="glyS"
                     /locus_tag="VN518_00680"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012318137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HXE03119.1"
                     /translation="ARMQPRAADDLQRIAGEKLAAAGLSFSAANSFVTPRRLTLLVDG
                     LPTAQPDVSEERRGPRVGAPPQAVEGFLKSAGISSLDQCEQRDTGKGVFYFANIRRAG
                     RATADVLPALLRALVVEFPWPKSMRFPAAGFRWVRPLTSALCLFDGKVLPLDLDGVPV
                     GATTGGHRFLSPTVFTVTGFADYRQKLRDARVILRAEDRAKAISLALYERAATADLTI
                     KDDAALLEEVTGLVEWPVVLMGAIDPAFLDLPPEVLTTSMRTHLKYFTCLDREGRLAP
                     RFLLVSNMMADDDGNAIVAGNERVLRARLSDARFFWEQDGKVRLASRVDKLKERVFHA
                     RLGNMRLKAQRIEWLSPNLALHVRGSDSSIAVRAAELCKADLSTGMVGEFPELQGIMG
                     RYYALKDGEAPAVADAIAQHYSPLGPNDSSPTAPNSVVVALADKIDTLASFFAIEEKP
                     TGSKDPFALRRAALGVIRIILENGLRIPLRDIFRYAYAPIGQFLASSPREHNYRQYLA
                     KKHDRSKQLRNENIERDLAGKLLAEILERQGASLADDLLAFFADRLKVHLREQGVRHD
                     LIAAVFALGNEDDLVRLLARVAALDGFLKTEEGANLLVAYRRAANIVRIEEKKDGRSY
                     AEPPDPKLLAEPAEAALAAALERAGARSAAALGREAFTDAMAALAVLRRPVDEFFARV
                     TVNCDNRDLRANRLRLLARIRDTLNQVADFSQIEG"
     gene            2164..>2531
                     /locus_tag="VN518_00685"
     CDS             2164..>2531
                     /locus_tag="VN518_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018260793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HXE03120.1"
                     /translation="MAKWVYSFGGGSAEGRADMKNLLGGKGANLAEMSNLALPVPPGF
                     TITTEVCTYFYANNKSYPKDLKEQVAAALKKLESAIAAKFGDAENPLLVSVRSGARAS
                     MPGMMDTVLNLGLNDVTIEGL"
BASE COUNT          447 a          853 c          781 g          450 t
ORIGIN      
        1 ccgcgcgcat gcagccgcgc gcggccgacg atctccagcg catcgccggc gagaagcttg
       61 ccgcggcggg cctcagcttt tccgccgcaa acagcttcgt cacgccgcgc cggctgacgc
      121 tgctcgtcga cggcctgccc accgcgcagc ccgatgtcag cgaggaacgc cgcgggccgc
      181 gcgtcggcgc gccgccgcaa gccgtcgagg gcttcctcaa atcggcaggc atttcctccc
      241 tcgatcaatg cgagcagcga gataccggca agggtgtgtt ctactttgcc aacatccgcc
      301 gtgccggccg ggccaccgcc gatgtcctgc cggctctgct gcgcgccctc gtggtcgagt
      361 ttccttggcc caaatccatg cgcttcccgg ctgcgggatt ccgctgggtg aggccgctca
      421 ccagcgccct ctgcctgttc gacggcaaag tgctgccgct cgatctcgac ggcgtgcccg
      481 tcggcgccac gacgggcggc catcgcttcc tctcccccac cgtcttcacc gtcaccggct
      541 tcgccgacta ccggcagaag ctgcgcgacg cccgcgtgat cctgcgcgcc gaagatcgcg
      601 ccaaggcgat ctcgctcgcg ctttatgagc gggcggccac agccgacctc accataaagg
      661 atgatgcggc cttgctcgag gaagtaaccg gactcgtcga atggccggtc gtgctgatgg
      721 gcgccatcga tccggccttc ctcgacttgc cgccggaagt tctcaccacc tcgatgcgca
      781 cgcatctcaa atacttcacc tgcctcgacc gcgagggcag gctcgcgccg cgcttcctcc
      841 tcgtctccaa catgatggcg gatgatgacg gcaacgcgat cgtcgccggc aacgagcgcg
      901 tgctgcgcgc ccgcctctcc gacgcgcgct tcttctggga gcaggacggc aaggtgagac
      961 tggccagccg tgtggacaag ctcaaagagc gggtctttca tgcgcggctg ggcaacatgc
     1021 ggttgaaggc ccagcgtatt gaatggttga gccctaacct cgctctacat gtccgaggca
     1081 gtgacagctc gatcgctgtt cgagccgctg aactgtgtaa agctgaccta tcgaccggta
     1141 tggtgggcga gtttccggag cttcagggca tcatgggacg ttactatgcc ttgaaagacg
     1201 gtgaagcgcc cgccgttgct gatgccattg ctcagcacta ttctccgcta ggtccgaacg
     1261 acagttctcc tacggcgcct aacagcgtcg tcgtggccct cgcagacaag attgataccc
     1321 tagcgtcatt ctttgcgatc gaggagaagc ctacaggatc taaggatccc tttgctcttc
     1381 gccgagcggc tctgggagtg atcaggatca tcctagaaaa cggccttcgt atcccgctca
     1441 gagacatctt ccgatacgca tacgcgccga ttggccaatt cctcgcgtca agcccgcggg
     1501 agcacaacta ccgccaatac cttgcgaaaa aacacgaccg cagcaaacag ttgcggaacg
     1561 agaacattga acgcgatttg gcgggtaagc tgctcgccga gatattggaa cgccaggggg
     1621 cgtcgctggc agacgatctc ctggccttct tcgccgaccg cctcaaggtc catctgcgcg
     1681 aacagggggt gcgccacgat ctcatcgccg ccgtgttcgc gttgggcaac gaggacgacc
     1741 tcgtgcgcct cctcgcccga gtcgccgcgc tcgacgggtt cctcaagacg gaggagggtg
     1801 ccaatctcct ggtcgcctat cgccgcgccg ccaatatcgt gcgaatcgag gagaagaagg
     1861 acggccgctc ctatgccgag ccgcccgatc cgaagctcct ggccgagccg gccgaagccg
     1921 ccctggcggc ggcgctggag cgggcgggag cacgtagcgc cgcagcgctc gggcgggagg
     1981 cctttaccga cgcgatggcg gccctggctg ttttgcgccg tccggtcgat gaattttttg
     2041 cccgcgtaac cgttaattgc gacaataggg acctgcgtgc gaaccggctg cggctcttgg
     2101 cccggatccg cgacacgctc aaccaggtcg ccgacttctc gcagatcgaa gggtaggtgg
     2161 gtaatggcga agtgggtcta cagctttggc gggggcagcg ccgaagggcg cgccgacatg
     2221 aagaacctgc tcggcggcaa gggcgccaac ttggccgaga tgagcaacct ggcgctcccg
     2281 gtcccgcccg gcttcaccat caccaccgag gtctgcacgt atttctacgc caacaacaag
     2341 agctacccga aggatctgaa ggagcaggtc gccgcggcgc tgaagaagct cgaatccgcg
     2401 atcgccgcca agttcggcga tgccgagaac ccgcttctcg tctcggtgcg ctcgggcgcg
     2461 cgcgcctcga tgcccggcat gatggatacg gtgctcaacc ttggcctcaa cgacgtcacc
     2521 atcgagggcc t
//
LOCUS       DATNAE010000038         2355 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_940759, whole genome shotgun sequence.
ACCESSION   DATNAE010000038 DATNAE010000000
VERSION     DATNAE010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940759"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1217
                     /locus_tag="VN518_00690"
     CDS             <1..1217
                     /locus_tag="VN518_00690"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HXE03121.1"
                     /translation="LEEIGAKAGSIVLEPCGRNTAPASCIAALIAARIDPQALVLLAP
                     SDHMIPDAEAFARTIESGLDAAAAGALVTFGIVPDCPHTGYGYIETEAGDGDTLKVQR
                     FVEKPSLAAAEQFLDSGKFYWNGGMFLFKASTMLDLLETHAPDILAGCRDSLAEVSED
                     LGFRVLNGAYSSVPAISLDYAVAEKAGNLTCVPLTTAWSDVGSWSSVWDFMDKDPSGN
                     VLGGTGETVVENARDNYVFSDHGCVALVGVENLVVVAMQDAVLVASKDEVERIKQVVE
                     RLKVNGCDLATLHNRVHRPWGWYQSLNRGDRYQVKCIMVKPGGRLSLQSHFHRSEHWV
                     VVSGTVEVTRGETVEMLSENQSTYIPVGEKHRLGNPGKIPAFLIEVQSGAYLNEDDIV
                     RYDDVYRRSSSE"
     gene            complement(1283..1358)
                     /locus_tag="VN518_00695"
     tRNA            complement(1283..1358)
                     /locus_tag="VN518_00695"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1323..1325),aa:Phe,seq:gaa)
     gene            complement(1485..1706)
                     /gene="yacG"
                     /locus_tag="VN518_00700"
     CDS             complement(1485..1706)
                     /gene="yacG"
                     /locus_tag="VN518_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HXE03122.1"
                     /translation="MSARSRGAEGSGKAAKRSAKALKCPICGKPRSEAFKPFCSKHCA
                     DVDLGRWLGGRYSVPGAPVASNEGEQDED"
     gene            complement(1703..2320)
                     /locus_tag="VN518_00705"
     CDS             complement(1703..2320)
                     /locus_tag="VN518_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HXE03123.1"
                     /translation="MLASASPRRLALLEQVGITPDALRPATIDESVAKGEVPRHIVRR
                     LARAKAEAALKLIRGDKDYGKAFLLAADTVVAVGRRVLGKAELEHEAGDALRLLRGRA
                     HRVHTAICLLTPDGRWRERVVETRVRFRNLSNEEIGNYLASGEWKGKAGGYAIQGIAG
                     SFVVKMVGSYTNVVGLPLTEVVSLLQGEGFPVSHLWLSKAEVETE"
BASE COUNT          420 a          783 c          738 g          414 t
ORIGIN      
        1 agctggagga gatcggcgcg aaggccggct cgatcgttct cgagccgtgt ggccgaaaca
       61 ccgcgcccgc atcatgcatt gccgcgctga tcgccgcgcg gatcgatccc caggcgctcg
      121 tgctgctcgc cccctccgac cacatgatcc ccgacgcgga ggctttcgcc aggaccatcg
      181 agagcggcct tgacgccgcc gcggcgggcg cgctcgtcac cttcgggatc gtccccgact
      241 gcccgcatac gggctacgga tatatcgaaa ccgaggcagg cgacggcgac accctgaagg
      301 tccagcgttt cgtcgagaag ccctcgctcg ctgccgccga gcaatttctc gacagcggca
      361 aattctattg gaacgggggc atgtttctgt tcaaggcgag caccatgctc gacctcctcg
      421 agacgcacgc tccggacatt cttgccgggt gtcgggactc tctcgccgag gtcagcgagg
      481 atctcggctt tcgcgtcttg aacggagcct atagcagcgt gccggccatt tctctcgact
      541 acgccgtcgc cgagaaggca ggcaacttga cgtgcgtgcc gttgaccacc gcatggagcg
      601 acgtcgggtc gtggtcctcg gtgtgggatt tcatggacaa ggatccgagc ggaaacgtgc
      661 tcggcggcac cggagagacc gtcgtcgaga atgcgcgcga caactatgta tttagcgacc
      721 atggctgcgt ggcgttggtt ggggttgaaa atctggtcgt ggtggcgatg caggacgctg
      781 tcctcgttgc ctccaaggac gaggtcgagc gcatcaagca ggtagtcgag cggctgaaag
      841 tcaacggttg cgatctggca accctgcaca atcgagtgca ccggccgtgg ggctggtatc
      901 aaagtctcaa tcgcggcgat cggtatcagg tcaaatgcat catggtgaag cctggcggcc
      961 gcctgtcgtt gcagagccac tttcatcgct cggagcactg ggtggtggtg tccggcacgg
     1021 tcgaggtgac gcgcggcgag acggtggaaa tgttgagcga gaatcaatcg acctacattc
     1081 cggtcggaga gaaacatcgc ctcggcaacc ccggcaagat tcccgctttc ctgatcgagg
     1141 tccagtcggg agcctatctg aacgaggacg atatcgtcag atacgacgac gtctatcgcc
     1201 ggagctcgag cgaatagcgc ctgcggtcac tggctgctac cgccggcccc gcaaacatcc
     1261 gcctaggcga ttgaagggga aatggtgccc aggggcggat ttgaaccacc gacacgcgga
     1321 ttttcagtcc gctgctctac caactgagct acctgggcct attgggcaat ttagtgaggc
     1381 gcgcgcgctc ccgccaggcg ggaggccata gcctcggacg gcaagacctt ggcttataga
     1441 aaatcgtctc gcccctgtcg agactaggat cgcgccccct cgcgctaatc ctcgtcctgc
     1501 tcaccctcgt tgctcgcaac cggcgcgccc ggcaccgaat agcggccgcc gagccagcgg
     1561 ccaagatcga cgtcggcgca atgcttcgag cagaacggct tgaacgcctc ggatcgcggc
     1621 ttgccgcaga tcgggcactt gagcgccttg gccgatcgtt tcgcggcctt gccggaccct
     1681 tcggccccgc gggagcgcgc gctcactcgg tctcgacctc ggctttcgac agccagagat
     1741 ggctcaccgg aaagccctcg ccttgcagca ggctcaccac ctcggtcaac ggcaaaccca
     1801 ccacgttggt ataggaaccc accatcttca ccacgaacga cccggcgatg ccctggatcg
     1861 cataaccgcc cgccttcccc ttccactcgc cgctcgccag ataattgccg atctcctcgt
     1921 tggagagatt gcggaacctg acgcgcgtct cgaccacccg ctcgcgccac ctgccgtcag
     1981 gggtcagcag gcagatggcg gtgtgcacgc gatgcgcgcg cccgcgcagc agtcgcaacg
     2041 cgtcgcccgc ctcatgctca agctcggcct tgccgagcac gcgccggccg accgccacca
     2101 cggtatcggc ggctagcagg aaggccttgc catagtcctt gtcgccacgg atcagcttga
     2161 gcgccgcttc ggccttggcg cgcgccagcc gccgcacgat atgccgcggt acctcgccct
     2221 tggccacgct ttcatcgatg gtggcggggc ggagcgcatc gggcgtgatg ccaacctgct
     2281 cgagcagcgc caagcgccgc ggcgaggccg acgccagcac gagctttggc ctgaacgcgc
     2341 tcggctgcgc acgcg
//
LOCUS       DATNAE010000039         3567 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_4252790, whole genome shotgun sequence.
ACCESSION   DATNAE010000039 DATNAE010000000
VERSION     DATNAE010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3567
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4252790"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="VN518_00710"
     CDS             <1..430
                     /locus_tag="VN518_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03124.1"
                     /translation="HTKETLGILADEGYTWSGDQCDDDNPYVVEVDGKRMVIVPKHWY
                     FNDWRAWAGGANSGAHAVEAFTDAFDFVYEEALRGKPGRIDALVHAELGGRPYMAHAF
                     EKMIKYLKRYEDEIWFPTRAEIADYMLRTVNEAKPYKPLG"
     gene            432..1151
                     /locus_tag="VN518_00715"
     CDS             432..1151
                     /locus_tag="VN518_00715"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXE03125.1"
                     /translation="MALALITGGGSLIGEGIACCLAEEGWEVVVSDIDRARAELVAAA
                     IGEHAEPLGLDVTHRTEVTAAVDAIEKRHKQIDALVNCAGGFRGLGLTRKPFAETEPA
                     EWDRILDANLKGVLHCCHAVLPGMMRAGAGSIVSISASRGLRGGPMAALYSAAKAAII
                     VFSQSLAQEVGKHGIRVNSIAPGSAEARWKALTAEDARGPMGRATTGRDVGNAVAFLL
                     SDRAKHVTGSCLDVSGGTALH"
     gene            1311..2516
                     /locus_tag="VN518_00720"
     CDS             1311..2516
                     /locus_tag="VN518_00720"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXE03126.1"
                     /translation="MATATTKTSAIETYLAGLQGEQLLVPVSWEQWLGIEAPYPTCGV
                     TRREVTARYARNGYEWDMHGSLFLPERERDAGFAVTMFHGGAGSENIFDKTPDGRPGL
                     GRVLASQGITALAMTYPGHWAPNPGGLWKIPVAERQPIYLLDRTPAPEEIADRNLKCT
                     FNTILQGAGQLVDTHLAGRKILSFGHSTGGPMAAHLHRFAKQVEAIGIVGFGSGGPDG
                     WRRTWRLETGAEKAKLFELDHVSRRSPKSFRASGYEDPADLCPWGGAEGYIGWAESIR
                     SQMKTSLCDNQHLGNTEIFPDYAARTGLPLAEYADHMQDPDPAWLKKIAVLLVVGDND
                     KGHWHHGGDTVAHKREPWMGAKYRAMGVRHAHVPVIARYGHVGYCELYNEKIAYLWLW
                     AYETGYFRR"
     gene            2534..3523
                     /locus_tag="VN518_00725"
     CDS             2534..3523
                     /locus_tag="VN518_00725"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXE03127.1"
                     /translation="MAKNIPLTLACGDYEIIRPLKEGTVKPDGIDLTVLTKMDSTTRH
                     WRFLRNQEFDVAEVSCSSYLVARDQGHKLDAIPVFLHRRFRHGFIFINTSKGIKSPKD
                     LIGKKIGVKQFQATAILWMRGILEHEYGVPHRSIDWYSELDESIDFEKPKDLKLTRLA
                     NDKSVEKMLAEGELDAVLHPDLIKPLIDKDPRVGRLFPNPMEEEIRYYKKTGIFPIMH
                     VMGIKRDIVEAHPWVPINLYHAFNEAKKMSMRRLENPRIVPLVFYRDYWEEQEEIFGN
                     DPWEYGLSNQNTHNLETLVTYSHEHGMIKKKMGLDELFLSVSQGRKRGEEFRI"
BASE COUNT          663 a         1176 c         1164 g          564 t
ORIGIN      
        1 tcacaccaag gagacgctcg gcatcctcgc cgacgaaggc tacacctggt cgggcgacca
       61 gtgcgacgac gataacccct atgtggtgga agtcgatggc aagcgcatgg tgatcgtgcc
      121 caagcactgg tacttcaacg attggcgcgc ctgggccggc ggcgccaaca gcggcgcgca
      181 tgccgtcgag gccttcaccg atgccttcga tttcgtgtac gaagaagcgt tgcgcggcaa
      241 gccggggcgc atcgacgcgc tggtccatgc cgagctcggc ggccggccct atatggcgca
      301 tgccttcgag aagatgatca agtacctcaa gcgctatgag gacgagatct ggttcccgac
      361 gcgcgccgag atcgccgatt acatgctgcg cacggtgaac gaggccaaac cctacaagcc
      421 gctcggttag catggcactc gcactcatca ccggcggcgg cagcctgatc ggcgagggca
      481 tcgcctgctg cctcgccgag gaaggctggg aggtcgtggt ctccgacatc gaccgcgccc
      541 gcgccgagct ggtcgcggcg gcgatcggcg agcatgccga accgctgggg ctcgatgtca
      601 cccaccgcac ggaggttacg gccgccgtcg acgcgatcga gaagcggcac aagcagatcg
      661 atgcgctggt caattgcgcc ggcggctttc gcggcctcgg cctcacgcga aaacccttcg
      721 ccgagaccga gcccgccgaa tgggaccgca tcctcgacgc caatttgaag ggcgtgctgc
      781 attgctgcca tgccgtgctg cccggcatga tgcgcgccgg agcgggctcg atcgtcagca
      841 tctcggccag ccgcggcctg cgcggcgggc cgatggcggc gctctattcg gcggccaagg
      901 cggcgatcat cgtcttcagc cagtcgctcg cccaggaggt cggcaagcac ggcattcgcg
      961 tcaactcgat cgcgccgggc agcgccgagg cacgctggaa ggcgctcacc gccgaggatg
     1021 cgcgggggcc catggggcgc gccaccaccg gccgcgacgt cggcaacgcc gtcgccttcc
     1081 tcctctccga ccgcgccaag cacgtcaccg gcagctgcct cgacgtgtcg ggcggcacgg
     1141 cgctgcattg atgggtgggt cgctgattgg tttttttgtg ccgtcatgcc cgggcttgac
     1201 ccgggcatcc acgtctttgc cggtgacgag cgccgacagc gcgctccgca cgctgagcgt
     1261 ggatggccgg gtcaagcccg ggcatgacgc ttgaaggaaa ggagcgaaga atggcgacgg
     1321 cgaccacgaa aacctctgcc atagagacct atctcgccgg gctgcagggc gagcagctgc
     1381 tcgtcccggt gagctgggag caatggctcg gcatcgaggc gccctatccc acttgcggcg
     1441 tcacgcgccg cgaggtgacg gcgcgctatg cgcgcaacgg ctatgagtgg gacatgcacg
     1501 gctcgctgtt cctgccggag cgcgagcgcg atgcgggttt cgccgtcacc atgttccatg
     1561 gcggcgccgg cagcgagaac atcttcgaca agacgcccga cgggcggccg gggctcggcc
     1621 gcgtgctggc gagccagggc atcaccgcgc tggccatgac ctatcccggc cattgggcgc
     1681 cgaatccggg cggcttgtgg aagatcccgg tggcggagcg ccagccgatc tacctgctcg
     1741 accgcacgcc ggcgcccgag gaaatcgccg accgcaattt gaaatgcacc ttcaacacca
     1801 tcctgcaggg cgccggccag ctggtcgaca cgcatctcgc cggccgcaag atcctctcct
     1861 tcggccattc gacgggcggg ccgatggcgg cgcatctgca ccgcttcgcc aagcaggtcg
     1921 aggcgatcgg catcgtcggc ttcggctcgg gcggccccga cgggtggcgc cggacatggc
     1981 ggctcgagac cggcgccgag aaggcgaagc tgttcgagct cgaccatgtc tcgcgccgct
     2041 cgcccaagag cttccgggcg tcgggctacg aggatccggc cgacctctgc ccctggggcg
     2101 gggccgaggg ctatatcggc tgggccgaga gcatccgctc gcagatgaaa accagcctgt
     2161 gcgacaacca gcatctcggc aacacggaga tcttccccga ttatgcggcg cgcaccggcc
     2221 tgccgctcgc cgaatatgcc gaccatatgc aggatcccga tcccgcctgg ctgaagaaga
     2281 tcgccgtgct gctggtggtc ggcgacaatg acaagggcca ttggcaccat ggcggcgaca
     2341 ccgtcgcgca caagcgcgag ccctggatgg gcgccaaata ccgcgccatg ggcgtccgcc
     2401 acgcccatgt gccggtcatt gcgcgctacg gccatgtcgg ctattgcgag ctgtataacg
     2461 agaagatcgc ctatctctgg ctctgggcct acgagacggg ctatttcagg cgctgaacca
     2521 ggagacagat ccgttggcca agaacatccc cctcacgctc gcctgcggcg attacgagat
     2581 catccggccc ttgaaggagg gcacggtgaa gccggacggc atcgacctca ccgtgctgac
     2641 caagatggat tcgacgaccc gccattggcg cttcctgcgc aaccaggaat tcgacgtggc
     2701 cgaggtgtcc tgctcgtcct acctggtggc gcgcgaccag ggccacaagc tcgatgccat
     2761 cccggtcttc ctgcatcgcc gcttccgcca cggcttcatc ttcatcaata cgagcaaggg
     2821 catcaaaagc cccaaggacc tcatcggcaa gaagatcggg gtgaagcagt tccaggcgac
     2881 ggcgatcctg tggatgcgcg gcatcctcga gcatgaatat ggcgtgccgc accgctcgat
     2941 cgactggtac agcgagctcg acgaatcgat cgatttcgag aagccgaagg atttgaagct
     3001 cacgcgcctg gccaacgaca agagcgtcga gaagatgctg gccgagggcg agctcgacgc
     3061 ggtgctgcat cccgacctca tcaagcccct gatcgacaag gatccgcgcg tcggacggct
     3121 cttccccaac ccgatggagg aggagatccg ctactacaag aagaccggca tcttcccgat
     3181 catgcatgtg atggggatca agcgcgacat cgtcgaggcg catccctggg tcccgatcaa
     3241 cctctatcac gccttcaacg aggcgaagaa gatgtcgatg cgccggctcg aaaatccgcg
     3301 catcgtgccg ctggtcttct atcgcgacta ttgggaggag caggaggaga tcttcggcaa
     3361 cgacccctgg gaatacgggc tttccaacca gaacacgcac aatctcgaga cgctcgtcac
     3421 ctattcgcac gagcacggca tgatcaagaa gaagatgggg ctcgacgagc tcttcctgtc
     3481 ggtgagccag ggccgcaagc gcggcgaaga gtttcggatc tagcgacctg aaccctctcc
     3541 cgcgaagagc gggagaggga ggggccc
//
LOCUS       DATNAE010000040         2411 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_345263, whole genome shotgun sequence.
ACCESSION   DATNAE010000040 DATNAE010000000
VERSION     DATNAE010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345263"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /locus_tag="VN518_00730"
     CDS             complement(<1..810)
                     /locus_tag="VN518_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HXE03128.1"
                     /translation="MTVLTPSAKDLKAIEAPFDVQAIRADFPILSRKVNGKNLVYLDN
                     GASAQKPRFVLETVQRAYGQDYANVHRGLHYLSNLSTANFEKARESVRRFLNARSDAE
                     IIFARNATEAINLVASSYGVPHIGEGDEILLTIMEHHSNIVPWHFLRERHGAVLKWAP
                     VSDNGDFLLDAFVKLITPRTKLIAITHMSNVLGTVVPLKEVVKIAHERGIPVLADGAQ
                     AAVHLPVDVQDLDVDFYAFTGHKLYGPTGIGVLYGKKERLERMRPYQGGGEM"
     gene            complement(821..2173)
                     /gene="sufD"
                     /locus_tag="VN518_00735"
     CDS             complement(821..2173)
                     /gene="sufD"
                     /locus_tag="VN518_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HXE03129.1"
                     /translation="MDLPVQQFRTKAEQAFLDMFEAAGKALPGAGDPFVSGLRRQAIE
                     TYGRLGLPHRRIEAWKYTDLRARLTDVNPLLKAEGIAVSEADLARALGGELAGLPAYR
                     LVVVEGDLRADLSHIADLKAAGVEVLSLGQALENPPSWLKAALAQVNPRKDDAVVALN
                     AALMTGGAAIRIGAGALLDKPIHLIELDGKGDPATTVTRNVVIAETGSSATLIESFAG
                     LGMRGLQRNAVTEIRVGDKAALRHVKLERDGEDALHLSTALIELAGDARYDAFQFSTG
                     ASLSRSQGYLAFMGEHSAADISGAFLMRGQQHCDTTLLVEHRVPHCLSRELFKGVLDN
                     EARGVFQGKIIVSPGAQKTDGKQMASALLLSELAEFDSKPELEIFADDVVCGHGSTSG
                     QIDKELLFYLEARGIPEGEARALLIQAFVGEALEKVEDEALRDALAHASAAWLGVEVE
                     "
     gene            complement(2187..>2411)
                     /gene="sufC"
                     /locus_tag="VN518_00740"
     CDS             complement(2187..>2411)
                     /gene="sufC"
                     /locus_tag="VN518_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003522658.1"
                     /note="part of SUF system involved in inserting
                     iron-sulfur clusters into proteins; in Escherichia coli
                     this protein forms a complex with SufBD; the SufBCD
                     complex stimulates the cysteine desulfurase SufS in
                     conjunction with SufE; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HXE03130.1"
                     /translation="LDIDAIRIVADGVNALRSPERATLVITHYQRLLDYIVPDRVHVL
                     SDGRVARSGGPELALELEQTGYADFGGKAA"
BASE COUNT          414 a          768 c          816 g          413 t
ORIGIN      
        1 catctcgccg ccgccctgat agggccgcat gcgctcgagc cgttccttct tgccgtagag
       61 cacgccgatg ccggtcggcc catacagctt gtggccggtg aaggcataga aatcgacgtc
      121 gagatcctgc acatcgacgg ggagatgcac ggcggcttgc gcgccgtcgg caagcacggg
      181 gatgccgcgt tcatgcgcga tcttcaccac ctccttcagc ggcacgacgg tacccagcac
      241 attcgacata tgcgtgatgg cgatcaactt ggtgcgcggg gtgatcaact tgacgaacgc
      301 gtcgaggagg aaatcgccgt tgtcggacac cggcgcccat ttcaggaccg cgccgtgccg
      361 ttcgcgcagg aaatgccacg gcacgatatt ggagtggtgc tccatgatgg tgagcaggat
      421 ctcgtccccc tcgccgatat gcggcacgcc ataggacgag gcgacgagat tgatcgcttc
      481 ggtggcgttg cgggcgaaga tgatctcggc atcggatcgc gcattgagga aacggcgaac
      541 gctctcgcgc gccttctcga aattggcggt cgagaggttg ctcagataat gcagcccgcg
      601 atgcacattg gcgtagtctt ggccataggc gcgctggacg gtctcgagca cgaagcgcgg
      661 cttctgcgcc gaggcgccgt tatcgagata gacgaggttc ttgccgttga ccttgcggga
      721 gaggatgggg aaatcggcgc ggatcgcttg cacgtcgaac ggcgcttcga tcgccttgag
      781 atccttggcc gagggtgtga gcacggtcat cgccgcatcc tcattcgacc tcgacgccga
      841 gccaggcggc ggaagcgtgg gccagcgcat cgcgcagcgc ctcgtcctcg accttctcga
      901 gcgcctcgcc gacaaaggcc tggatcagaa gtgcccgggc ttcaccctcg gggatgccgc
      961 gcgcctcgag atagaacaga agctccttgt cgatctggcc cgaggtcgag ccatggccgc
     1021 agaccacgtc gtcggcgaag atctcaagct ccggcttgga atcgaactcg gccaattcag
     1081 acagcagcag cgcgctggcc atctgcttgc cgtcggtctt ctgcgcaccg ggcgagacga
     1141 tgatcttgcc ctggaacacg ccgcgcgcct cgttgtcgag cacgcccttg aacagctcgc
     1201 ggctcaagca atgcggcacg cggtgctcga ccaagagcgt ggtgtcgcaa tgttgctggc
     1261 cgcgcatgag gaacgcgccg gagatgtcgg cggcggaatg ctcccccatg aaggccagat
     1321 agccctggct gcgagagagc gaagcgccgg tcgagaactg gaaggcgtcg tagcgcgcat
     1381 cgccggcaag ctcgatcagc gcggtcgaaa gatgcagcgc atcctcgccg tcgcgctcga
     1441 gcttcacatg gcgcagcgcg gccttgtcgc cgaccctgat ctccgtcacg gcgttccgct
     1501 gcaggccgcg catgccgagg ccggcgaagc tttcgatcag cgtggccgag ctgcccgtct
     1561 cggcgatcac tacgttgcgc gtgacggtcg tggccggatc gcccttgccg tcgagctcga
     1621 tgagatggat tggcttgtcg agcaaggctc cggcgccgat gcggatggcg gcgccaccgg
     1681 tcatcaaagc ggcgttgagc gccaccacgg catcgtcctt ccgcgggttg acctgggcga
     1741 gagccgcctt gagccaggac ggcgggttct cgagcgcctg gccgagcgag agtacctcca
     1801 cgccagccgc cttgaggtcg gcaatgtggg acaggtccgc cctaagatcc ccttcgacca
     1861 ccaccagccg ataggccggc aggccggcga gctcgccgcc aagggcgcgg gcgaggtcgg
     1921 cttcgctcac cgcgatgcct tcggccttca gcagcggatt gacgtcggtc agccgggcgc
     1981 gaaggtcggt gtatttccag gcctcgatgc ggcgatgcgg caggccgagc cggccgtagg
     2041 tctcgatggc ctggcgccgc aagcccgaca cgaatggatc gccggcgcct ggaagcgcct
     2101 tcccggccgc ctcgaacatg tcgagaaagg cctgttcggc cttggtcctg aattgctgga
     2161 cggggagatc catcgttccg ctttcgctag gcggccttgc cgccgaaatc ggcgtagccc
     2221 gtctgctcga gctcgagcgc aagctcaggg ccacctgagc gcgccacgcg cccgtccgag
     2281 agtacatgca cgcggtccgg cacgatgtaa tcgagcagcc gctgataatg ggtgatgacg
     2341 agcgtggcgc gctcgggact gcggagcgca ttgacgccgt cggcgacgat gcggatcgcg
     2401 tcgatgtcga g
//
LOCUS       DATNAE010000041         3259 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_1548692, whole genome shotgun sequence.
ACCESSION   DATNAE010000041 DATNAE010000000
VERSION     DATNAE010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1548692"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..1224
                     /locus_tag="VN518_00745"
     CDS             190..1224
                     /locus_tag="VN518_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HXE03131.1"
                     /translation="MSNVVGPKDASPGLSRAHALAALRAAGEATRLRILALLAGGELN
                     VKDLTQILGQSQPRISRHLKLMAEAGLIKRFREGSWVYSRLAESGAEAALAEAIVESL
                     DQTDLTLARDRSRATAVQKARAEAAQAYFKAHASEWDSIRALHVAERDVEAAMDEALG
                     EGPFHLLVDLGTGTGRILELFAARADRALGFDLNHAMLAYARMKLERAGLSHAQVRHG
                     DLHNVPLPDGAADAVVLHQVLHFLDDPAAAVAEAARLLAPGGKLLVVDFAPHELEFLR
                     EQSAHRRLGFAKDQLGRLFEGVGLKLERFRELAPRPSGGKLTVSLWLGQKPAASGRKK
                     RIGAQVEVAA"
     gene            1224..2144
                     /gene="metF"
                     /locus_tag="VN518_00750"
     CDS             1224..2144
                     /gene="metF"
                     /locus_tag="VN518_00750"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HXE03132.1"
                     /translation="MLASSEPRSRILGRGAIDVSFEFFPPKTAAMEETLWSSIRRLEP
                     LRPKYVSVTYGAGGSTRERTHATVARILSETTLKPAAHLTCVAATKGEVDEITRDYWD
                     LGVRHIVALRGDPPDGAGTRYEPTPGGYVNAADLVKGIKHIAPFEISVACYPEKHPES
                     PSVEADIDMLKAKIDAGATTAISQFFFDNEVFFRYLDRVRAAGITIPIVPGIIPIHNF
                     KQCSGFAAKSGASVPDWIGRRFEGLDDDQETRHLVAAAVAAEQVLGLVDQGITEFHFY
                     TLNRADLVYAICHLLGLRPAKAFAAAKAAS"
     gene            2141..3202
                     /locus_tag="VN518_00755"
     CDS             2141..3202
                     /locus_tag="VN518_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HXE03133.1"
                     /translation="MTREERIAALKREAEQRILILDGAMGTMIQRYKLDEAGYRGTRF
                     KKHPRDLKGNNDLLVLTQPKIISEIHNAYLEAGADIIETNTFNAQAISQADYGLEDVA
                     YEMNVAAAKIAREAADAWTEKTPDQPRFVAGAIGPTNRTASISPDVNNPGFRNVSFDA
                     LVDAYTTQTRGLIEGGADTILIETVFDTLNAKAAGFAVEQAFEQLGGELPIMISGTIT
                     DLSGRNLSGQTPEAFWYSMQHLKPFSIGLNCAFGAEQLRPSVDEMAHVADTYVSVYPN
                     AGLPNEMGEYDESPEFMAALLETWAKDGLINIVGGCCGTTPDHIRAIAAAVSKYKPRH
                     VPEVSHKLRLSGLEPFVHG"
BASE COUNT          591 a         1092 c         1017 g          559 t
ORIGIN      
        1 caaagaggcg caagctcact tgatcgaaga agaactttcg gttgattgcg ctcgtcgtcg
       61 cctccctcgg tccccgatta tagcgtgcgc gtagttttgt gcatgctcgc ggcacgaggc
      121 gcaaaaaatg ggcgaaacca atgggcttcg cccttgtaag tctcgcaaaa cacgtataaa
      181 catatcttta tgtcgaatgt cgtcggaccc aaagatgcca gcccgggcct gtcccgcgcg
      241 cacgcgctgg cggcgttgcg cgccgccggc gaggcgacgc gcttgcgcat cctggcgctg
      301 ttggccggcg gcgagctcaa cgtcaaggat ctgacccaga tcctcggtca gagccagccg
      361 cgcatcagcc ggcatctcaa gctcatggcc gaggccgggc tcatcaagcg cttccgcgag
      421 ggctcctggg tctattcccg acttgccgag tctggcgccg aggccgcgct ggcggaggct
      481 atcgtcgaga gcctcgacca gaccgacttg acgctcgccc gcgaccgctc gcgcgcgacc
      541 gccgtgcaga aagcgcgcgc ggaagcggcc caagcttatt tcaaggcgca tgcgagcgag
      601 tgggactcga tccgcgcgct gcatgtggcg gagagagacg tcgaggccgc catggacgag
      661 gcgttgggtg aggggccgtt ccatctcctc gtcgacctcg gcaccggcac tggccgcatc
      721 ctcgagctgt tcgccgcccg cgccgatcgc gcgctcggct tcgatctcaa tcacgcgatg
      781 ctggcctatg cgcgcatgaa gctggagcgc gccggactca gccacgcgca agtccgtcac
      841 ggcgatctgc acaatgtgcc gcttcctgac ggcgcggcgg atgcagtggt cttgcatcag
      901 gtgctgcatt tcctcgatga cccggcggct gcggtcgccg aagccgcgcg gctgctcgcc
      961 cccggcggca aactgctggt ggtcgacttc gccccgcatg agctcgagtt cctgcgcgag
     1021 caatcggcgc atcggcggct cggcttcgcc aaggatcagc tcgggcggct gttcgaaggc
     1081 gtggggctga agctcgagcg cttccgcgag ttggcgccca ggccctcggg cggcaagctg
     1141 accgtatcgc tgtggttggg ccagaagccc gcggcctcgg gcaggaagaa gcgcatcggc
     1201 gctcaggtgg aggtcgccgc gtgatgctgg catcctccga gcccagaagc cgcattctcg
     1261 gccgcggcgc catcgacgtg tcgttcgagt tcttcccgcc caagacggcg gcgatggaag
     1321 agacgctgtg gagctcgatc cggcggctcg agccgttgcg tcccaaatat gtgtcggtca
     1381 cctatggcgc gggcggctcg acgcgtgagc gcacccatgc cacggtggcg cgcatcctga
     1441 gcgagaccac cttgaagccg gcggcgcatc tcacctgcgt cgcggcgacc aagggggagg
     1501 tcgatgagat cacccgcgac tattgggatc tcggcgtgcg ccacatcgtg gccttgcgcg
     1561 gcgatccgcc cgacggcgcc ggcacgcgct acgagccgac ccctgggggc tatgtcaacg
     1621 ccgccgatct ggtcaaaggc atcaagcaca tcgcgccctt cgagatctcg gtcgcctgct
     1681 atccggagaa gcaccccgag tcgccgagcg tcgaggccga tatcgacatg ctgaaagcca
     1741 agatcgatgc cggcgctacg accgcgatca gccagttctt cttcgacaac gaggtgttct
     1801 tccgctatct cgatcgcgtc cgcgcggccg gcatcaccat tcccatcgtg cccggcatca
     1861 ttcccatcca caatttcaag caatgctccg gcttcgccgc caaatccggc gcgagcgtcc
     1921 ccgattggat cggccgtaga ttcgaaggcc tcgacgacga tcaggagacg cgccatctgg
     1981 tggctgccgc cgtcgccgcc gaacaggtgc tcgggctggt cgatcagggt attacggagt
     2041 ttcacttcta cacgctgaac cgcgccgacc tcgtctatgc catctgccat ttgcttggct
     2101 tgcgcccggc gaaggcgttc gccgcagcaa aggccgcatc atgacccgtg aagaacgcat
     2161 tgccgcgctg aagcgggaag ccgagcagcg catcctcatc ctggatggcg ccatgggcac
     2221 catgatccag cgctacaagc tggacgaggc cggctatcgc ggcacgcgct tcaagaagca
     2281 cccgcgcgat ctcaagggca acaacgatct gctcgtgctc acccagccga agatcatcag
     2341 cgagatccac aacgcctatc tggaagccgg cgccgacatc atcgagacca acaccttcaa
     2401 cgcccaggcc atcagccagg cggattacgg cctcgaggac gtcgcctacg agatgaatgt
     2461 cgccgcggcg aaaatcgccc gcgaggcggc cgacgcctgg accgagaaga cgccggatca
     2521 gccgcgcttc gttgccggcg ccatcggccc caccaaccgc accgcctcga tctcgcccga
     2581 tgtgaacaat cccggtttcc gcaatgtcag tttcgatgcg ctggtcgacg cctacaccac
     2641 gcagacccgc ggcctgatcg aaggcggcgc cgataccatc ctcatcgaga cggtgttcga
     2701 tacgctgaac gccaaggcgg cgggttttgc cgtggaacag gcgttcgagc agcttggcgg
     2761 cgagctgccc atcatgatct ccggcaccat caccgatctt tccgggcgca atctctcggg
     2821 ccagaccccc gaggccttct ggtattccat gcagcatttg aagccgttct cgatcggcct
     2881 caattgcgcc ttcggcgccg agcagctcag gccatcggtc gacgagatgg cgcatgtggc
     2941 cgacacctat gtcagcgttt atcccaatgc cgggcttccg aacgaaatgg gcgagtatga
     3001 cgagagccct gaattcatgg cggcattgct ggagacctgg gcgaaagacg gcctgatcaa
     3061 cattgtcggc ggctgttgcg gcacgacgcc cgaccacatc cgcgctatcg ccgcggccgt
     3121 atccaaatac aagccgaggc acgtgccgga ggtgagccac aagctccgcc tttcgggcct
     3181 cgaacccttc gtgcatggat agacaatcga cgtgaccaac ggcgcacaga atttcgtcat
     3241 ggtgggcgag cggaccaac
//
LOCUS       DATNAE010000042         2768 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_1245345, whole genome shotgun sequence.
ACCESSION   DATNAE010000042 DATNAE010000000
VERSION     DATNAE010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1245345"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VN518_00760"
     CDS             <1..223
                     /locus_tag="VN518_00760"
                     /inference="COORDINATES: protein motif:HMM:NF017897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HXE03134.1"
                     /translation="TALLLGLLTINPVIVLAGTALGYLCAWTGHFLIEKNRPAMVSHP
                     AWAFQCDIRMFRLWLTGQLGPELARAGVA"
     gene            complement(220..813)
                     /locus_tag="VN518_00765"
     CDS             complement(220..813)
                     /locus_tag="VN518_00765"
                     /inference="COORDINATES: protein motif:HMM:NF017833.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HXE03135.1"
                     /translation="MLRDFVASETGPRISLSALRDALDDRGFGVLLFVFAFPNLLPHN
                     IPLVSAVLGLPLVLLAAQLSYGRHKPWFPSWLAERSFPRLGFTAIVMHALPYLERAER
                     LLKPRLTGLLSWTGERFIGFAILVLAIALTLPIPFGNFLPAFAIAIVGLALVEKDGVA
                     ALVGLGVGAASLIVVGLVVGGLIAATRLLLSSLFGSA"
     gene            complement(927..1619)
                     /locus_tag="VN518_00770"
     CDS             complement(927..1619)
                     /locus_tag="VN518_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03136.1"
                     /translation="MRQTVLCAAMVVALLADVPALAAEDAATDAARPSEAAPDTGVGA
                     KRVLTPEEQAEKDARKECKKKICDIIATRDPNGEDVACDITKTWREEDIVKMLGGKIG
                     WPWGKAVCQSRLELKRKPLALAMSEAEYEIVMPAQKVRCTLAQKDKGEPYAIEITLAP
                     KVRFKDGKASEATLNWGEASAPMFIYPLIYAATGFDNSSNVLGPEVVRLVNEFTTKKC
                     AEVKAEAPAGTP"
     gene            complement(1629..2735)
                     /gene="leuB"
                     /locus_tag="VN518_00775"
     CDS             complement(1629..2735)
                     /gene="leuB"
                     /locus_tag="VN518_00775"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019965982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HXE03137.1"
                     /translation="MTTYKLLLLPGDGIGPEVMGEVSRLVSWINAQGLAKLETETDLV
                     GGCAYDSHGEAISEATMNRALAADAVMLGAVGGPKWDGVAYDKRPEAGLLRLRKDLQL
                     FANLRPAICYPALADASSLKRDRVDGLDIMIVRELTGGVYFGEPKTITDLGNGQKRAV
                     DTQVYDTYEIERIAAVAFDLARERKRLVHSAEKRNVMKTGVLWNEVVTKVHKERYGDV
                     TLHHILADNCAMQLVRQPKQFDVIVTDNLFGDMLSDEAAMLTGSLGMLPSASLGEADA
                     KGHRRALYEPVHGSAPDIAGQGRANPIATIASLAMCLRYSFFLGDIADRIDGAIAQVL
                     DQGLRTADIAQDGCRTVGTTEMGEAIIAALNRGL"
BASE COUNT          459 a          919 c          916 g          474 t
ORIGIN      
        1 cacggcgctg ctcttgggcc tgctcaccat caatccggtc atcgtgctgg caggcacggc
       61 gctcggctat ctctgcgcct ggacggggca cttcctcatc gagaaaaacc gcccggccat
      121 ggtctcgcat ccggcctggg ccttccaatg cgacatccgc atgttcaggc tgtggctcac
      181 ggggcagctc ggccctgagc tggcgcgggc cggcgtggcc taggccgatc cgaacaggct
      241 cgacagcagc aaccgggtgg cggcgatcaa accgccgacc acgaggccga ccacgatcag
      301 gctggcggct cccacgccga ggcccaccag cgcggcgacg ccatccttct cgaccagagc
      361 gaggccgaca atggcgatgg cgaatgccgg caggaaattg ccgaaaggga tcggcaaagt
      421 cagcgcgatg gcgagcacga gaatggcgaa gccgataaag cgctcgccgg tccacgacaa
      481 taatcccgtc aagcgcggct tgagcaggcg ctcggcccgt tccagatagg gcaaagcgtg
      541 catgacgatg gcggtgaagc cgagccgcgg gaaagaccgc tccgccagcc aggaggggaa
      601 ccagggcttg tgccggccgt aggacaactg cgcggcgagc aagaccagcg gcaggccgag
      661 cacggcggag acgagcggga tgttgtgcgg cagaagattg ggaaaggcga agacaaacag
      721 cagaacgccg aaaccgcgat cgtcgagcgc gtcgcggagt gcgctaaggc tgatgcgcgg
      781 tcccgtctcg cttgccacaa agtcgcgaag caactgcgac gtgcgccgcg tcggcgataa
      841 gtcgtgttcg cccggagccg ggccctttgt cgggatagcg tcttcctgaa ccgtcaaacc
      901 gtcttctctt tcgccgcgcc ccccgattat ggagtgccgg ccggcgcctc ggccttcacc
      961 tcggcgcatt tcttggtggt gaactcgtta accagacgca ccacctcggg gccaagcacg
     1021 ttgctcgaat tgtcgaagcc ggttgccgca tagatcagcg gataaatgaa catcggcgcc
     1081 gacgcctcgc cccaattgag cgtcgcctcc gacgccttgc cgtccttgaa cctcaccttg
     1141 ggcgccagcg tgatctcgat ggcatagggc tcgcccttgt ccttctgggc gagggtacag
     1201 cggacctttt gcgccggcat gacgatctcg tattcggctt cgctcatcgc cagagcgagc
     1261 ggcttgcgct tgagctcgag ccgcgactgg cacaccgcct tgccccaggg ccagccgatc
     1321 ttgccgccga gcatcttgac gatgtcctcc tcgcgccagg tcttggtaat gtcgcacgcc
     1381 acgtcctcgc cgttcggatc gcgcgtggcg atgatgtcgc agatcttctt cttgcattcc
     1441 ttgcgcgcgt ccttctcggc ctgctcctcc ggcgtgagca cgcgcttggc gccgacgccg
     1501 gtatccggcg ccgcctcgct tggccgtgcc gcgtcggttg ccgcatcctc ggcggcgagt
     1561 gccgggacat cagcgagcag cgccacgacc atcgccgcgc aaaggaccgt ctgtctcatg
     1621 cttcaccctc acagtccgcg gttgagggcc gcgatgatgg cctcgcccat ttcggtcgtg
     1681 ccgacggtgc ggcagccgtc ctgggcgatg tcggcggtgc gcaggccctg gtccagcacc
     1741 tgcgcgatgg ccccgtcgat ccgatcggca atatcgccga ggaagaagga ataacggaga
     1801 cacatggcga ggctggcgat ggtggcgatc ggattggcgc gaccttgccc cgcgatatcg
     1861 ggcgccgagc catgcaccgg ctcgtacagc gcgcgcctgt ggcccttggc atcggcctcg
     1921 ccgagcgagg cggagggcag catgccgagc gagccggtca gcatcgccgc ctcgtccgac
     1981 agcatgtcgc cgaacagatt gtcggtgacg atgacgtcga actgcttcgg ctgacgcacg
     2041 agctgcatgg cgcaattgtc ggcgaggata tggtgcagcg tcacgtcgcc atagcgctcc
     2101 ttgtgcactt tggtgaccac ctcgttccac aacaccccgg tcttcatgac gttgcgcttc
     2161 tcggcggaat gcaccaggcg cttccgctcg cgcgccaggt cgaaggcgac cgcggcgatg
     2221 cgctcgatct cgtaggtgtc gtagacctgc gtatcgacgg cgcgcttctg gccgttgcca
     2281 agatcggtga tggtcttggg ctcgccgaaa tagacgcctc cggtcagttc gcgcacgatc
     2341 atgatgtcga ggccgtcgac tcggtcgcgc ttgagcgagg aggcgtcggc gagcgcgggg
     2401 tagcagatcg cgggccggag attggcgaac agctggagat ccttgcgcag ccggagcagc
     2461 ccggcctccg ggcgcttgtc gtaagccacg ccatcccatt tcggcccgcc gaccgcgccg
     2521 agcatgacgg cgtcggcggc gagcgcgcgg ttcatggtcg cctcggagat ggcctcgcca
     2581 tgcgagtcgt aggcgcagcc gccgaccagg tcggtctcgg tctccagctt ggcgaggccc
     2641 tgcgcgttga tccaggacac aaggcgggac acctcgccca tcacctcggg gccgatgccg
     2701 tcgccgggga gcagcagaag cttataggtc gtcatggctg cagcgctcct ttgccgtttc
     2761 ccctggcc
//
LOCUS       DATNAE010000043         2036 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3356582, whole genome shotgun sequence.
ACCESSION   DATNAE010000043 DATNAE010000000
VERSION     DATNAE010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3356582"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2036
                     /locus_tag="VN518_00780"
     CDS             <1..>2036
                     /locus_tag="VN518_00780"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019170441.1"
                     /note="Catalyzes the rate-limiting step in dNTP synthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HXE03138.1"
                     /translation="AGQAAPRRRRQDGLQFDTTINEWHTCPNDGRINGSNPCSEYMFL
                     DDTACNLASINLLPFRKADSSFDVAAFEHACRLWTIVLEISVMMAQFPSRAIAERSFE
                     YRTLGLGYANVGGLLMTSGIPYDSAEGRAIAGAITAVMTGIAYKTSAEMAGELGAFGD
                     YKRNAKHMLRVIRNHRNAAHGNAGGYEGLSIAPVPLDHASLKDDALGARAKSAWDEAL
                     ALGEKHGYRNAQVSVIAPTGTIGLVMDCDTTGIEPDFALVKFKKLAGGGYFKIINRAV
                     PPALRTLGYSESQIAEIEAYAVGHGNLNQAPGINPATLKAKGFDDDKIEALNKALGSA
                     FDIKFAFNQWTLGADFLKGLGVTDAQLADFSFDLLGFLGFSKKDIEAANVHVCGAMTL
                     EGAPFLKAEHLPVFDCASPCGKLGKRYLSVESHILMMAAAQPFISGAISKTINMPNDA
                     TVEDCKESYMLSWRLALKANALYRDGSKLSQPLNASLLADDEEDEDEAVEAALAAAPM
                     ARVPQVTERIVEKIVEREVAVREREKMPYRRKGYTQKSIVGGHKVYLRTGEYEDGRLG
                     EIFIDMYKEGAAFRAMLNNFAIAISLGLQYGVPLDEYVEAFTFTRFEPAGMVIGNDRI
                     KNATSMLDYIFRELAISYLDRDDLAHVPADAAPTALSRGSAETTARDSQGPTAV"
BASE COUNT          383 a          710 c          613 g          330 t
ORIGIN      
        1 aagcaggtca agctgcgcct cgtcggcgtc gacaagatgg gttgcagttc gacaccacca
       61 tcaacgagtg gcacacctgc ccgaacgacg gccggatcaa cgggtcgaac ccgtgctccg
      121 agtacatgtt cctcgacgac acggcctgca acctcgcctc gatcaacctc ctgcccttcc
      181 gcaaggcgga ttcgagtttc gacgtcgcag ccttcgagca cgcctgccgg ctctggacga
      241 tcgtcctcga gatctcggtg atgatggcgc agttcccgag ccgcgcgatc gccgagcgct
      301 ccttcgaata ccgcacgctc ggcctcggct atgccaatgt cggcggcctc ttgatgacct
      361 ccggcatccc ctatgactcg gcggaaggcc gcgccatcgc cggcgcgatc accgcggtca
      421 tgaccggtat cgcctacaag acctcggcgg agatggccgg cgagctcggc gcgttcggcg
      481 actacaagcg caacgccaag cacatgctgc gcgtcatccg caaccaccgc aacgcggcgc
      541 acggcaacgc gggcggatat gaagggcttt ccatcgcccc cgtgccgctc gaccatgcct
      601 cgctcaagga cgacgcgctc ggtgcgcgcg ccaagtcggc gtgggacgag gcgctggcgc
      661 tcggcgagaa gcacggctat cgcaacgccc aggtctccgt catcgcgccc accggcacga
      721 tcggcctcgt gatggactgc gacacgaccg gcatcgagcc cgatttcgcg ctggtaaaat
      781 tcaagaagct cgccggcggc ggctatttca agatcatcaa ccgcgccgtg ccgccggccc
      841 tgcgcacgct cggctattcg gagagccaga tcgccgagat cgaggcttat gccgtcggcc
      901 acggcaatct caaccaggcg ccgggcatca accccgccac cctcaaggcc aagggctttg
      961 acgacgacaa gatcgaggcg ctgaacaagg ccctcggctc ggccttcgat atcaaatttg
     1021 ccttcaacca gtggacgctg ggcgccgact tcctcaaggg cctcggcgtc accgacgcgc
     1081 aactcgccga tttctccttc gatctgctcg gcttcctcgg cttttcgaag aaagacatcg
     1141 aagccgccaa cgtccatgtc tgcggcgcca tgacgctcga gggcgcgccc ttcctcaagg
     1201 cggagcacct gccggtgttc gactgcgcca gcccctgcgg caagctcggc aagcggtatc
     1261 tgtcggtcga gagccacatc ctgatgatgg cggcggcgca gcccttcatc tcgggcgcga
     1321 tctccaagac catcaacatg cccaacgacg ccacggtcga ggattgcaag gagagctaca
     1381 tgctctcctg gcggctggcg ctgaaggcca acgcgctcta tcgcgacggc tccaagcttt
     1441 cccagccgct caacgcctcg ctcctcgccg acgacgagga ggatgaggac gaggcggtcg
     1501 aagccgccct tgccgcggcg ccgatggccc gggtgccgca ggtcaccgaa agaatcgtcg
     1561 agaagatcgt cgagcgcgaa gtcgccgtgc gcgagcgcga aaagatgccg tatcgccgca
     1621 agggctacac gcaaaaatcc atcgtcggcg gccacaaggt ttatctcagg accggcgaat
     1681 acgaggatgg ccggctcggc gagatcttca tcgacatgta caaggaaggc gccgccttcc
     1741 gcgccatgct gaacaatttc gccatcgcga tttcgctcgg cctgcaatat ggcgtgccgc
     1801 tcgacgaata tgtggaggcg ttcaccttca cccgcttcga gccggccggc atggtgatcg
     1861 gcaacgaccg gatcaagaac gccacgtcga tgctcgacta catcttccgc gagctcgcca
     1921 tctcctatct cgatcgcgac gacctggcgc atgtgccggc cgacgcggcg ccgacggcgt
     1981 tgagccgcgg ctcggccgag accacggcgc gcgattcgca aggcccgacg gccgtt
//
LOCUS       DATNAE010000044         6675 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_346684, whole genome shotgun sequence.
ACCESSION   DATNAE010000044 DATNAE010000000
VERSION     DATNAE010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6675
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346684"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VN518_00785"
     CDS             complement(<1..213)
                     /locus_tag="VN518_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018115699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HXE03139.1"
                     /translation="MNAAAAEDPTPEPEQLPVGLKPIRYLDALNPEQRESVETLDGPL
                     LVLAGAGTGKTRVLTTRIAHILSLGRA"
     gene            557..796
                     /locus_tag="VN518_00790"
     CDS             557..796
                     /locus_tag="VN518_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03140.1"
                     /translation="MSRQLAAQSAAELRRSKGWSYVSVSAYRVKPDPFWKSVRPVVPK
                     DVIYGSFVTAKTYTTCFTGVVVPYVCTSGSKVCGN"
     gene            956..1603
                     /locus_tag="VN518_00795"
     CDS             956..1603
                     /locus_tag="VN518_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HXE03141.1"
                     /translation="MTGVKFPQYTLRERVADGCIHVVGVAASVVALTALLIVGVQAQA
                     TLWIVSLSIYGLALVTMFTCSAGYNLIVRPKVKAVFRRLDHAAIFLMIAGTYTPFALM
                     KMDAEWGYTLLAVVWTMAAIGIAIKLFAPRYLEGVTTALYLVQGWAVIVAWHPLVSAV
                     PERVAFLLMLGGVLYTVGVVFHLWERLPFQNAIWHGFVLAAASVHYAAVIDIVGS"
     gene            1668..3083
                     /locus_tag="VN518_00800"
     CDS             1668..3083
                     /locus_tag="VN518_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HXE03142.1"
                     /translation="MSAFMADAVERPPSEAKRALFARLAARFGNRFSTGAALREQHAN
                     TLTWIATQPPDAVLFAETTPEVVEAVKLCAEARVPVIPFGTGTSLEGHVNAPFGGVSL
                     DLSRMNRILAVHEEDLDCVVEPGVTRKALNDHLRDQGLFFAIDPGADASLGGMAATRA
                     SGTNAVRYGTMRDNVLSLNAVLADGTLLNTGQRARKSASGYDLTHLLIGSEGTLGIIT
                     ELTLKLHGIPETILSAVCPFATLEGACNATIIAMQMGLPLARIELADEVQIRACNAYS
                     HLTLAEQPTLFLEFHGSATATREQVETFAEIAREQGGGQFAWAERQEDRSKLWQARHD
                     AYWAARGLRPGAEVVTTDVCVPISALAACVGETRADIDRTGLLAPIVGHVGDGNFHVM
                     PLFDAGSTEERRAVQGFLDRLTERALLMQGTCSGEHGVGQGKIRYLVQEHGAGVDVMI
                     AIKKALDPLNILNPGKIFALP"
     gene            3176..>6675
                     /locus_tag="VN518_00805"
     CDS             3176..>6675
                     /locus_tag="VN518_00805"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HXE03143.1"
                     /translation="MNSFLLAFTAVLILVLSALFAAPLFIDWNDYRPVFETQASKLLG
                     RDVKVGGKVHMVLLPAPELRFDQVKVADEKGSFGQPLLEAKSIEAWLNIGALLRGAIE
                     ARKIAITEPVLRLTLREDGTGNWSDVGRAGQALPFVPKEVLLDQVSVSFGRVEVSRPG
                     MKPLAVDNVSGEASAASLSGPYKVTATYDFAGRPQELRFSTGASDAEGVFRLKSALRD
                     PDRNIIYLLEGDVAGLRDKPSYDGAFIMRIASALPAEAAPPQQGQAQKPAEAPADGTP
                     SAEPNPGAEAHLSAFELKGELKATPDHAELMAFEIAIHSKGRSQMMKGKLALDFGAQA
                     KAVGEIAASWIDVDTLLAAAAPANAQSEPSTADVLSAAAERALDEAASVGDGALTVKL
                     DQASIGCDLVGALDLAVAAKDGVVTVERLNAELPGENRIEASGLLTRGEGGPVFEGPI
                     KLEGSKLKTLARWVAGDRETSGQATVGAFTLSAKTTIAAGNLMLDDASGELSGTKFSG
                     ALHYRGGERRVVDLTLDSDRLDLREVMGESAAWRNWLPDSDAKQPDFSPVSEQSLLAA
                     LRDDEVHATLRVGELLLPNIPAGRLDAKFSLAKDTLDVERLDFAAPGAIALNGNGRIE
                     RLSDAPAGKVDLSLQAMTADGLKVTSELLGLGDGVTKSKQLASLAPLDLHMGLTSVRE
                     GNATSAAIEVKGKAGGTDVSILAKATGDPGKLADAAIAIDGSVEGNEPQILLGFLVPG
                     LAPERLAVAGGDRGTFTLKAQGVPSKSVTGHGELATSDLQLTFDGEGALQPEGVGLSG
                     QASAKSSNAGMALAVLGLDASPSAAVPLDLRADVVKTAGSIDFKSISGEIAGEPVGGS
                     ASFDTSGDRTRFRVAADTGSVSLPALLGSLIAWQRTPSTEEMLGSLAQNASQVWPARG
                     FALEPLMNAEGEILLNAKELAVGAPFQVEDATLTAHVDQKGLSVTDLQGRLFGGSFAA
                     SGTLTPKGTGAELKLHAELAGGRMDQLSQSLAGRVLAKGPFTFVVDVSGEGLSPPGLV
                     AGLTGEGALFLDPGALQALSPEPLKRVALDANRSKKIKLDKDQIAARMSTLQDTLTRG
                     TYAYAGTALPFGIKNGTLKLDPAVLVGKGAEATINGYVELASLRLDSEWAMRLNGSKD
                     ADVPPVNLVLAGSLQDPAAIT"
BASE COUNT         1148 a         2194 c         2129 g         1204 t
ORIGIN      
        1 agcccggccc aagctcaaaa tatgggcaat tctcgtggtg agcacgcgcg tcttgccggt
       61 cccggcgccg gcaagcacga gcagcggccc gtcaagcgtc tccaccgact cgcgctgctc
      121 ggggttgaga gcatcgagat agcggatggg tttcagaccc acggggagtt gctcgggctc
      181 cggcgtgggg tcttcggccg cggctgcgtt cacgcgaatc tttgacattg ctttcaaact
      241 atacatcggg cttgcgggaa aaagggcaag accttgcccc cggcgagcag aggtgtgact
      301 tcgttgcatc gcgggcgatt cgccacacgc tgagcacgac aagtttggtt gccagcgcgc
      361 cgccggactg gcaaaaaagg cgctattgcg gggcctgatt cttcactggg actgggggaa
      421 aagtatgctt cgatctgcga agattattgt gcttgccatc cttgcattag cggtatgggg
      481 gcagcatccg gcggcggctg gctgtcaggt tatctcggcg acccatagcg ccgacaccaa
      541 agccgaggcc ttgaccatgt cgcgacaact cgcggcccag agcgccgctg agttgcgtcg
      601 ctccaagggc tggagctatg tcagcgtgag cgcttacaga gtgaagcccg atccgttctg
      661 gaagtcggtg cggccggtgg tgccaaagga cgtgatctac gggtctttcg tcacggcgaa
      721 gacctacacg acctgcttca ccggcgtggt ggtgccttac gtctgcacct ccggttccaa
      781 ggtctgcggc aactaggctc tttcacctcc ggaactgcga tcagaccatt gccgttgtga
      841 acagcggcgc ggagcttctc cgcgccacaa acggcgttcg cgcgcctcac ccttgccaca
      901 acaaatggta aggtgcttta cacgttcctt tgttctgata ggtccgggat tggtcatgac
      961 tggcgtgaag tttccgcaat acacattgcg cgagcgtgtt gccgacggct gcattcacgt
     1021 ggtcggcgtt gccgcgagtg tggtggcgct caccgcgctt ctgatcgtgg gcgtgcaggc
     1081 gcaggctacg ctgtggatcg ttagcctctc gatctacggc ttggcactcg tcaccatgtt
     1141 cacctgctcg gccggttata acctcatcgt caggcccaag gtgaaggcgg tgtttcgccg
     1201 cctcgaccac gccgccatct tcctcatgat cgccggcacc tacacgccgt tcgcgctgat
     1261 gaagatggac gctgagtggg gctacacctt gctcgccgtg gtgtggacca tggcggccat
     1321 cggcatcgcc atcaagctgt tcgcgccgcg ctatctcgaa ggcgtcacca ccgcgctcta
     1381 tctggtgcag ggctgggcgg tgatcgttgc ctggcatcct ttggtgtcgg ccgtgccgga
     1441 gagggttgcg ttcctgctca tgcttggtgg cgtgctctac accgtcggcg tggtgttcca
     1501 tctctgggag cggcttccct tccagaatgc catctggcac ggtttcgtgc tggccgccgc
     1561 cagcgttcat tacgccgcgg tgatcgacat cgtcggctcc tgagcggcgg cgcccctatt
     1621 ccaaagcctt agcttttcgt gaatcgcgat ggtggtagag tgccgccatg agcgcattca
     1681 tggcagacgc tgtcgagcgg cctccatctg aggccaagcg cgccctgttc gcgcgcctcg
     1741 cagcgcggtt cggcaatcgc ttctcgaccg gcgcggcgct ccgcgagcag cacgccaaca
     1801 cgctgacctg gatcgccaca cagccgcccg acgcggtgct gttcgccgag accacgccag
     1861 aggtggtcga ggcggtgaag ctttgcgctg aggcccgcgt gccggtgatc ccgttcggca
     1921 ccggcacctc gctcgaaggc cacgtcaatg cgccgttcgg cggcgtgtcg ctcgaccttt
     1981 cgcgcatgaa ccgtattctc gccgttcacg aagaggacct cgactgcgtg gtcgagcccg
     2041 gcgtgacacg caaagccctg aacgatcact tacgcgacca aggcctcttc ttcgccatcg
     2101 acccaggcgc cgatgcgagc ctcggcggca tggcggcgac ccgtgcctca ggcaccaacg
     2161 ccgtgcgcta cggcaccatg cgcgacaacg tcttgagcct taacgcggtg ttggccgacg
     2221 gcacgctgct caacaccggg cagcgcgcac ggaaatcggc aagcggctac gacctcaccc
     2281 atctcctgat cggctccgag ggcacgctcg gcatcatcac cgagctcacg ctgaagctgc
     2341 acggtatccc cgagaccatt ctctccgcgg tctgcccctt cgccaccctc gaaggcgcct
     2401 gcaacgccac catcatcgcc atgcagatgg gactcccgct cgcccgcatc gagctcgccg
     2461 acgaggtgca gatcagggcc tgcaacgcct attcgcatct cacgcttgcc gagcagccga
     2521 cactttttct cgagtttcac ggcagcgcca ccgccacgcg cgagcaagtc gagaccttcg
     2581 ccgagatcgc gcgtgagcag gggggagggc agttcgcctg ggctgagcgc caggaggacc
     2641 gcagcaagct ctggcaggcg cgccacgacg cctattgggc ggcgcggggc ttgcgtccag
     2701 gcgccgaggt ggtaaccacc gatgtgtgcg tgccaatctc ggcccttgcc gcttgcgtcg
     2761 gcgagacgcg cgccgacatc gaccggaccg gcctgctcgc cccgatcgtc ggccatgtcg
     2821 gcgacggcaa tttccacgtc atgccgctgt tcgatgccgg cagtaccgag gagaggcgcg
     2881 cggtgcaggg ctttctcgac cggctgaccg agcgcgccct gctcatgcaa ggcacctgca
     2941 gcggcgagca cggcgtgggc cagggcaaga tccgctatct cgtgcaagag cacggcgccg
     3001 gcgtcgatgt gatgattgcc ataaaaaagg cgctagatcc gttgaatatc cttaatcctg
     3061 gtaagatctt tgcattacca tgacccaagt tgtgggcgga gaggaccaaa gtgtcccccg
     3121 gctttaccca atgccctcgc gaaagctcgc gatcgagcct aaggcataga tttccgtgaa
     3181 ctcatttctg ctcgccttta cggcagtgct gattttggtg ctgagcgcgc tcttcgcggc
     3241 gccgctcttc attgactgga acgattaccg tccggtcttc gaaactcagg cgagcaagct
     3301 gcttggacgc gacgtaaagg tcggcggcaa ggtgcatatg gtgctgctgc cggcgcctga
     3361 gcttcgcttc gaccaggtca aagtggccga cgagaaaggg agcttcggcc agcccctgct
     3421 cgaggcaaag tcgatcgaag cttggctcaa tatcggggct ctgctgcgcg gcgccatcga
     3481 ggcgcgcaag atcgcgatca ccgagccggt gcttcggctc acactgcgcg aggatggcac
     3541 cggcaattgg agcgatgtcg gcagggcagg gcaagcgctt cccttcgtgc ccaaggaggt
     3601 gctcctcgat caggtgagcg tgagctttgg gcgcgttgag gtgagccggc cgggcatgaa
     3661 gccgctcgcc gtcgataacg tctccggcga agcaagtgcc gcatcactgt ccgggccgta
     3721 caaggtgacg gcgacctacg atttcgcggg gcgcccccag gagcttcgtt tctcgaccgg
     3781 cgcgagcgat gccgagggcg tgttccgcct caagtcggcg ctgcgcgacc ccgatcgcaa
     3841 catcatttat ctgcttgaag gcgacgtggc cggtctccgc gacaagccga gctacgacgg
     3901 cgccttcatc atgcgcatcg cctcggccct tccggccgaa gctgcgcctc cccaacaggg
     3961 ccaagcacag aagcctgccg aggcgccggc cgacggcacg ccgtctgccg agccgaaccc
     4021 cggcgccgag gcccatctct cagccttcga gctcaagggc gagctcaagg cgacgcctga
     4081 ccatgccgag cttatggcct tcgaaattgc cattcactcc aaaggccgct cgcagatgat
     4141 gaagggcaag cttgcgctcg atttcggcgc ccaagccaag gctgtaggcg agatcgccgc
     4201 gagctggatc gatgtcgata cgctccttgc cgccgcggca cccgcgaatg cgcaaagcga
     4261 gccttcgacc gctgacgtgt tgagcgccgc cgccgaacgg gcgcttgacg aagccgcctc
     4321 ggtgggagac ggcgcgctga ccgtcaagct cgaccaggca agcattggct gcgatcttgt
     4381 cggtgccctc gacctcgccg tggcggccaa ggatggcgtg gtgacggtcg agcgcctcaa
     4441 cgccgagctt cccggcgaga accgcatcga ggcctccggt cttctcacgc gaggcgaggg
     4501 gggaccggta ttcgagggcc cgatcaagct cgaaggctca aagctcaaaa ccctcgcccg
     4561 ctgggtcgcc ggcgaccgcg agacgtcggg ccaggcgacc gtcggcgcct tcacactttc
     4621 cgccaagacc accatcgccg ccggcaacct catgcttgac gacgcgagcg gcgagctgag
     4681 cggcacgaag ttcagcggcg ccttgcatta tcgcggcggc gagcggcgcg tcgtcgatct
     4741 gaccctcgac agcgaccgcc tcgatctgcg cgaggtgatg ggcgagagcg cggcgtggcg
     4801 caactggtta cctgattccg atgcgaagca gccggacttt tcgccggtgt ccgagcaaag
     4861 cctgctcgca gcgctccgcg acgatgaggt tcatgccact ttgcgcgtcg gcgagcttct
     4921 gcttcccaac attcccgccg ggcggctcga cgcgaaattc agccttgcca aggacaccct
     4981 cgacgtcgag cgcctcgact ttgccgcgcc gggcgccatc gcgctcaacg gcaacggccg
     5041 catcgagcgc ttgagcgatg cgccggcagg caaggtcgat ctctctctgc aagccatgac
     5101 cgccgacggg ctgaaagtca cgagcgagct ccttgggctt ggtgacggcg tgaccaagtc
     5161 gaagcagctc gcaagcctcg cccctctcga tttgcatatg gggctcacgt cggtgcgcga
     5221 gggcaatgcc accagcgctg ctatcgaggt gaagggcaag gccggcggca ctgatgtctc
     5281 catcctcgcc aaggccaccg gcgacccggg caagctagcc gacgcggcaa tcgctatcga
     5341 tggcagcgtc gaaggcaacg agccgcagat ccttctcggc ttcctggtgc cgggcctcgc
     5401 gcctgagcgg ctcgccgttg ccggcggcga ccgcggtacg ttcacgctga aggcgcaagg
     5461 cgtgccgagc aaaagcgtca ccgggcatgg agagctcgcg acctccgact tgcaactcac
     5521 ctttgatggc gagggcgcgt tgcagcccga gggggtgggc ttgtccggcc aagcttcggc
     5581 aaaaagcagc aacgccggta tggcgcttgc ggttctcggc cttgacgcct cgccaagcgc
     5641 cgccgtgccg ctcgaccttc gcgccgatgt ggtcaagacc gcaggcagca tcgacttcaa
     5701 atcgatctca ggcgagattg cgggcgagcc cgtgggagga agcgcatcct tcgacacgag
     5761 cggggatagg acgcgcttca gggtcgcggc cgacacaggt tcggtttcgc tgccagctct
     5821 gcttggatcg ctcatcgcct ggcagcgcac gccgtcaact gaggagatgc tcggctcgct
     5881 cgcccaaaac gcctcgcaag tttggccggc gcgcggcttt gcgctcgagc cgcttatgaa
     5941 cgctgaaggc gagatcttgc tgaacgcgaa ggagcttgcc gtcggcgcgc ccttccaggt
     6001 cgaagacgcc acgctcaccg cccacgtcga tcagaagggg ctttccgtca ctgacttgca
     6061 gggacgcctg ttcggcggct cgttcgcggc ctctggaacg ctcactccga aaggcactgg
     6121 cgccgagctc aagcttcacg ccgagcttgc cggcggcagg atggaccagc tctcgcaatc
     6181 cctcgccgga cgcgtgctcg ccaaaggtcc gttcaccttc gttgtcgacg tctcaggcga
     6241 ggggctcagt ccccctggcc tcgtcgccgg gctgaccggc gagggcgctc tgtttctcga
     6301 tcccggcgcg ctgcaagcct tgtcgcccga gccgctgaag cgcgtagcgc tcgatgccaa
     6361 tcggtcgaag aaaatcaagc tggacaaaga ccagatcgcc gcgcggatga gcacgttgca
     6421 ggacacgctc acgcggggaa cttacgccta tgcgggcacc gcgctcccct tcggcatcaa
     6481 gaacggcacg ctcaagcttg accctgcggt gcttgtcggc aaaggcgcgg aggccaccat
     6541 caacggttat gtggaattgg ctagtctgcg gctcgacagc gagtgggcga tgcgcctcaa
     6601 cggcagcaag gacgcagacg tgcctccggt gaatctcgtc ctagccgggt ctctccagga
     6661 ccccgccgcc atcac
//
LOCUS       DATNAE010000045         2504 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153 k141_44276,
            whole genome shotgun sequence.
ACCESSION   DATNAE010000045 DATNAE010000000
VERSION     DATNAE010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44276"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="VN518_00810"
     CDS             <1..502
                     /locus_tag="VN518_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HXE03144.1"
                     /translation="WGQMPTVGTPEYDAWNKEVRAFGKAIWEVTGKGVNVDSVFEHPG
                     EATFPVSCFLVKRGGMVVFCAGTTGYNLTMDARCVWMHQKRIQGSHFAHLQQASSANK
                     LVVERRIDPCMSEVFPWEQIPKAHMRMWKNEHRPGNMSVLVSAPCTGLRTFEDVLEAS
                     KQMHGG"
     gene            535..1389
                     /locus_tag="VN518_00815"
     CDS             535..1389
                     /locus_tag="VN518_00815"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF037026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXE03145.1"
                     /translation="MGEPQGDYRIERMPFSGVETVIDWAAAEGWNPGLGDAACFYAID
                     PNGFFMGVLDGRPIARVSMPIYDDQFAFCGLYIVDPAFRGQGYGLGLTKASLDYIGSR
                     NAGLDGVEAMAAKYARLGYRRAHRSTRHSFIPKQQQAPMREIVSLSQVPFATLAAYDR
                     AHFFAPRDRFLERWISQPHAVGLALIEDAQLKGYGILRKCRVGHKIGPLFADAADIAE
                     ALFGALCNNALGEQVFIDIPEPNQAGLDLAAKHGMSPVLACERMYLRGDPGLPLEEIY
                     GMTSFEAG"
     gene            1487..2383
                     /locus_tag="VN518_00820"
     CDS             1487..2383
                     /locus_tag="VN518_00820"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HXE03146.1"
                     /translation="MLYDTVLLRTFVSICDSGSFTKAAREVNLTQSAVSLHIKRLEDQ
                     VGSRLIVRNARGVRLTENGEILLSYARRILALHKEAEHRLGRDSDSPIRIGAPDYFDF
                     HILSSLLGQFAARYPAVRLQVELGIGPDISALLDEGELDLAIVSHEIGEGDGVALCRE
                     RRVWAAAQAMRLDPSEPAPLALYPPHCRWRQLALEQLDRAGRAWTLVLQSAGTAGILA
                     ALDAGLGITIFPEYGLPSTLKALGSAEALPALPDFEFVLRRSRSRSSAADHLAGMITS
                     FFELSTALRPGIDYQERANFLL"
BASE COUNT          435 a          819 c          781 g          469 t
ORIGIN      
        1 ctggggccag atgccgaccg tcggcacgcc cgaatacgat gcttggaaca aggaagtccg
       61 cgccttcggc aaggcgatct gggaagtcac cggcaagggc gtgaacgtcg acagcgtgtt
      121 cgagcatccg ggcgaggcga ccttcccggt ctcctgcttc ctggtgaagc gcggcggcat
      181 ggtcgtgttc tgcgccggca ccaccggcta caacctcacc atggacgcgc gctgtgtgtg
      241 gatgcatcag aagcgcatcc aaggctcgca tttcgctcat ctccagcagg ctagctcggc
      301 caacaagctc gtcgtcgagc ggcgcatcga tccctgcatg agcgaggtct tcccctggga
      361 gcagattccc aaggcgcata tgcgaatgtg gaagaacgag caccgtcccg gcaacatgtc
      421 ggtgctggtg agcgcgcctt gcacgggctt gcgcaccttc gaggacgtgc tcgaggcgag
      481 caagcagatg cacggcggct aggcactcga gccgcaagcg taagcggcaa cggaatgggc
      541 gagccgcaag gcgattaccg catagagaga atgccgtttt cgggcgtcga aacggtgatc
      601 gactgggccg ccgctgaagg ctggaacccg gggctgggcg atgccgcctg tttctacgcc
      661 atcgatccca acggcttttt catgggcgtg cttgacggcc gcccgatcgc gcgcgtctcc
      721 atgccgatct acgacgacca gttcgcgttt tgcggccttt atatcgtcga tcctgccttt
      781 cgcggccagg gctacggcct cggcctgacc aaagcgagcc tcgactatat cggcagccgc
      841 aatgccggcc tcgacggcgt cgaggccatg gccgcgaaat atgcgcggct tggctatcga
      901 cgagcgcatc gcagcacgcg ccacagcttc ataccgaagc agcaacaagc tccgatgcgc
      961 gagatcgtct ccctctcaca agtgcccttc gccacgctcg cggcttacga ccgcgcacat
     1021 ttcttcgcgc cccgcgatcg gttcctcgag cgctggatca gccagccgca tgcggtcggc
     1081 ttggccttga tcgaggacgc ccaattgaag ggctacggga tcctgcggaa gtgtcgcgtc
     1141 ggtcacaaga tcggcccgct ttttgccgat gcggccgaca tcgccgaggc cttgttcggc
     1201 gcgctttgca ataatgcctt gggcgagcag gttttcatcg atattccgga gccgaaccag
     1261 gccggcctcg acctcgccgc caagcatggc atgagtccgg tactcgcctg cgagcgcatg
     1321 tatctgcgcg gcgaccccgg cctgccgctt gaggaaattt atgggatgac gagtttcgag
     1381 gcgggataac cgccaactcg gtctgcacca taatcacgca agccgtgcgt ccggcggtgc
     1441 aatataatcg caaggtctcc gataaagcct gagcggtcat aggctgatgc tctacgacac
     1501 cgtcctgtta cgaacttttg tatcaatctg cgactccggc agcttcacca aggcggcgcg
     1561 tgaggtcaat cttacgcaat cggcggtcag tctgcacatc aaacgcctgg aggatcaggt
     1621 tggctcgcgc ctgatcgtgc gcaacgcacg tggcgttcgg ttgacggaga atggcgagat
     1681 ccttttgtcc tacgcgcggc gcatcttggc gctgcacaag gaagccgagc atcgcttggg
     1741 gcgcgacagc gactccccga tccggatcgg cgctcccgat tatttcgact tccatatttt
     1801 gtcgtccttg ctcggccaat tcgccgcccg ctatcccgcg gtgcggctgc aagtcgagct
     1861 cggcatcggt cccgacatct cggcgcttct cgacgagggc gagctcgatc tcgccatcgt
     1921 cagccatgaa atcggcgaag gcgatggcgt cgcgctatgc cgcgagcgcc gcgtctgggc
     1981 cgccgcacaa gccatgcgcc tcgacccttc cgagccggcg ccgctcgctc tatatccgcc
     2041 gcactgccgc tggcggcagc tcgccttgga gcaactcgac cgggccgggc gggcatggac
     2101 gctggtgctg cagagcgccg gcaccgcggg catcctcgcc gcgctcgacg ccggcctcgg
     2161 catcacgatc tttccggaat atggcttgcc gtcgacgctg aaagcgctcg gcagcgcgga
     2221 agccttgccg gcactccccg acttcgaatt cgtgctgcga cgcagccgca gccgctcatc
     2281 ggccgctgac catctcgccg gcatgatcac cagcttcttc gagctctcga cggcgctgag
     2341 acccggcatc gattaccagg agcgcgcgaa tttcttgctc tgaacagcct ggaggctagt
     2401 cgccgcatcc ggatgagtgc tcatacactg tccaggggtc gctgtcgtgc gcgcggggct
     2461 tgcagggttg aaacgccgcc ttcggcggtg gctcgaccgg agcg
//
LOCUS       DATNAE010000046         2281 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_348152, whole genome shotgun sequence.
ACCESSION   DATNAE010000046 DATNAE010000000
VERSION     DATNAE010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348152"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1020
                     /locus_tag="VN518_00825"
     CDS             <1..1020
                     /locus_tag="VN518_00825"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009008 - DNA-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HXE03147.1"
                     /translation="LPAASVDLVFADPPYNLQLSGELRRPNHSRVDGVVEEWDRFADF
                     AEYDHFTRDWLGEARRLLKPNGGLWVIGSYHNIYRVGAILQDLGFWILNDIVWRKTNP
                     MPNFRGRRFTNAHETLLWCARSQEARYTFNYEAMKALNEELQMRSDWLIPLCGGPERL
                     KRDGKKAHPTQKPEALLHRVLLASTKPGDLVLDPFFGTGTTGAVAKRLGRHFIGIERE
                     KDYAQIARSRIAEVVPAPPDSVTMQAKREEPRIPFGTLIERGFLKPGDILIDASGRYR
                     AKVRADGTLISAEARGSIHQVGALVQGAPACNGWAFWCFEHQGKPVSIDVLRQRVRSE
                     MAASA"
     gene            complement(1114..2064)
                     /locus_tag="VN518_00830"
     CDS             complement(1114..2064)
                     /locus_tag="VN518_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HXE03148.1"
                     /translation="MPVLKVRDLAFARLRAPDLDAAEEFLDHFGMIKVERTPTALYYR
                     GTEPAHHIHITEKAESPSFAGLAYELAVGDDLKRAAKLPGASGIETIDEPGGGKRVRL
                     TEPNGYTIELVHGIARHERLPLAHQKLNIREERYSRVGEVMRIKPGAAHVMRIGHAVL
                     ASPRVRETVAWFREMLGLVGSDDVYAGTKDNLIGSFNRLDRGDDYVDHHVFFCITNPK
                     AGLNHLSFEVPDIDDVFMGHHHLKHQAKYDHMWGIGRHLLGSQVYDYWADPWGRVHEH
                     WADTDRLNASTPMNVISAEEGLTSQWGEPPPQRFIELACP"
BASE COUNT          396 a          758 c          727 g          400 t
ORIGIN      
        1 ctccccgccg cctcggtcga tctcgtcttc gccgatccgc cctacaatct gcaattgagc
       61 ggcgagctgc gccggcccaa tcacagccgc gtcgacggcg tggtggagga gtgggaccgc
      121 ttcgccgatt ttgcggagta cgaccacttc acccgcgact ggctcggcga ggcccggcgg
      181 ctcctgaagc cgaacggcgg cctctgggtc atcggcagct atcacaatat ctatcgcgtc
      241 ggcgccatcc tccaggatct cggattctgg atcctcaacg acatcgtctg gcgcaagacc
      301 aatccgatgc cgaatttccg cggccggcgc ttcaccaatg cgcatgagac gctcttgtgg
      361 tgcgcgcgca gccaagaggc gcgctacacc ttcaattacg aggcgatgaa ggcgctcaat
      421 gaagagctgc agatgcgcag cgactggctc attccgctgt gcggcgggcc ggagcggctg
      481 aagcgtgacg gcaagaaggc gcacccgacg caaaagcccg aagcgctcct gcaccgcgtg
      541 ctgctcgcct cgaccaagcc cggcgatctc gtcctcgacc ctttcttcgg caccggcacc
      601 acgggcgcgg tggcgaagcg cctgggccgc catttcatcg gcatcgagcg cgaaaaggac
      661 tacgcccaga tcgcccgctc gcgcatcgcc gaggtggtgc cggcgccccc cgacagcgtc
      721 accatgcaag ccaagcgcga agagccgcgc atccccttcg gcacgctcat cgagcgcggg
      781 tttttgaagc ccggcgacat cctgattgat gccagcgggc gctaccgcgc caaggtgcgc
      841 gccgacggca cgctcatctc cgccgaggcc cgcggctcca tccaccaagt gggggcgctg
      901 gtgcaaggcg cccccgcctg caacggctgg gccttctggt gcttcgagca tcaaggcaaa
      961 ccggtctcga tcgacgtgct gcgccagcgc gtgcgctcgg agatggcggc aagcgcgtag
     1021 cgaaatttct gctattcctc tcgtcgtcat gccccgcttg gcgcggccat gacgaaaggg
     1081 caaagaaccc ggcgcgtatc gaacgtacgt gcgctacggg cacgccagct cgatgaagcg
     1141 ttgcggcgga ggctcgcccc attgcgaggt caagccttct tcggcggaga tgacattcat
     1201 cggcgtgctg gcgttgaggc ggtcggtgtc ggcccaatgc tcatgcacac ggccccaggg
     1261 atcggcccag tagtcataga cctggctgcc gagcaaatgc cggccgatgc cccacatgtg
     1321 gtcatacttt gcctgatgct tcagatgatg gtggcccatg aagacatcgt cgatgtcggg
     1381 cacttcgaag gagagatgat tgaggccggc cttgggattg gtgatgcaga agaacacgtg
     1441 atggtccaca taatcatcgc cgcggtcgag ccggttgaat gagccgatga gattgtcctt
     1501 ggtcccggca tagacatcgt ccgagcccac aaggcccagc atctcgcgga accacgccac
     1561 ggtctcccgc accctgggcg aggccaacac ggcatggccg atgcgcatga catgggcggc
     1621 gccgggcttg atgcgcatga cctcgccgac gcggctgtag cgctcctcgc ggatgttgag
     1681 cttctgatgc gcgagcggga ggcgctcatg gcgcgcgatg ccgtgcacga gctcgatcgt
     1741 atagccattg ggctcggtca ggcgcacgcg cttgccgccg ccgggctcgt cgatcgtctc
     1801 gatgcctgag gcgccgggga gcttcgccgc gcgcttcagg tcatcgccga cggcgagctc
     1861 gtaggcgagg ccggcgaatg acgggctttc cgccttttcc gtgatgtgga tgtgatgggc
     1921 gggctccgtc ccgcggtaat agagcgcggt cggcgtgcgc tcgaccttga tcatgccgaa
     1981 atgatcgagg aattcctccg ccgcatcgag atctggtgca cgcaaccgag cgaaagcgag
     2041 atcccgtacc ttgagcaccg gcatggcttc ctccctggca gctctctccg ccgcctacga
     2101 gcgtctacga agcttaagat cgcgggcgcc gggcggcaag gaaagccacg cgccacggcc
     2161 cctcattgac gcacccggcg ccatccccta ctctattaat gacatgacca ccgcagggac
     2221 cctcggcctt cgacttatct gaccggcgct ttccggagcg tcgggctttg gccgttgtcg
     2281 t
//
LOCUS       DATNAE010000047         8875 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2456888, whole genome shotgun sequence.
ACCESSION   DATNAE010000047 DATNAE010000000
VERSION     DATNAE010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 8875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8875
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2456888"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VN518_00835"
     CDS             <1..327
                     /locus_tag="VN518_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HXE03149.1"
                     /translation="GGKIKEKLTGTEDLGYSYEIVESPLPVKNYKSKLWLEVDDEPDR
                     SVIYWQSDFDANGASDDDAKAKITGILADGVKGIKQQAITAHDAKEAAAGKTPEGDKD
                     KDDDDK"
     gene            complement(447..872)
                     /gene="rplQ"
                     /locus_tag="VN518_00840"
     CDS             complement(447..872)
                     /gene="rplQ"
                     /locus_tag="VN518_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HXE03150.1"
                     /translation="MRHRNSGRKLNRTASHRSALFSSLACALIKHEQIVTTLPKAKDL
                     RRVADRLITLAKKGDLHARRLAMSRIRDEAMVAKLFGTLGPRYADRSGGYTRVMKAGF
                     RYGDSAPMAVIELVDRDEAARGLDSGPVQERDAEAEKAA"
     gene            complement(909..1925)
                     /locus_tag="VN518_00845"
     CDS             complement(909..1925)
                     /locus_tag="VN518_00845"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HXE03151.1"
                     /translation="MLQKNWQDLIKPTKLNVSSGRDPARFALVVAEPLERGFGLTLGN
                     ALRRVLLSSLQGAAITSVHIDGVLHEFSSIAGVREDVTDIILNIKEIALRQHAEGVKR
                     MVLKKEGPGVAKAGDIETGADIEILNPDHVICTLDQGAAIRMEFTTSTGKGYVPAERN
                     RPDDAPIGFIPVDSLYSPVRKVSYRVENTREGQILDYDKLTMEIETNGSVRPEDTVAL
                     AARIVQDQLSVFVNFEEPKKEMPFERQPELVFNAALLKKVDELELSVRSANCLKNDNI
                     VYIGDLIQKTEAEMLRTPNFGRKSLNEIKEVLASMGLHLGMEVPDWPPENIEELAKRF
                     EDHY"
     gene            complement(2013..2402)
                     /gene="rpsK"
                     /locus_tag="VN518_00850"
     CDS             complement(2013..2402)
                     /gene="rpsK"
                     /locus_tag="VN518_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HXE03152.1"
                     /translation="MAKDKTRVRRREKKNITSGVAHVHASFNNTMITITDAQGNTISW
                     SSAGAMGFKGSRKSTPYAAQVAAEDAGKKAMEHGMKNLEIEVRGPGSGRESALRALQA
                     AGFTIITIRDVTPIPHNGCRPRKRRRV"
     gene            complement(2474..2842)
                     /gene="rpsM"
                     /locus_tag="VN518_00855"
     CDS             complement(2474..2842)
                     /gene="rpsM"
                     /locus_tag="VN518_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011651425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HXE03153.1"
                     /translation="MARIAGVNIPSNKRVQVALTYIHGIGDTFAKQILAKVGIPPERR
                     VSQLTESEVIQIREVIDRDYMVEGDLRRDVAMNIKRLMDLGCYRGLRHRRGLPVHGQR
                     THTNARTRKGPAKPIAGKKK"
     gene            complement(3024..3677)
                     /locus_tag="VN518_00860"
     CDS             complement(3024..3677)
                     /locus_tag="VN518_00860"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008124947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HXE03154.1"
                     /translation="MILVLLGPPGAGKGTQANLLETDRSLVKLSTGDMLRAAVAAGTE
                     LGRKAGDIMERGALVPDDIVIGLIAERLDRGGEGEGFILDGFPRTIAQAEALDRLLKE
                     RGKTLDAVVEMQVDDDALVKRIIGRFTCANCGEGYHDDFKTPKVPGVCDRCGSREFVR
                     RRDDNEEVVRSRLEAYHAQTKPLINYYAKQGKLKSVDGMAEIGEVRRAIGRALDGSA"
     gene            complement(3674..5008)
                     /gene="secY"
                     /locus_tag="VN518_00865"
     CDS             complement(3674..5008)
                     /gene="secY"
                     /locus_tag="VN518_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HXE03155.1"
                     /translation="MVSAAEQLVSNLNFAAFAKADELKKRIWFTLGALLIYRLGTYIP
                     IPGINPTALAEVFRQNQSGLLGMFDMFSGGAVGRMAIFALNIMPYISASIIIQLLTTV
                     SPHLEQLKKEGEQGRRQMNQYTRYGTVLLAALQAYGIAVGLEGAGNVVLDPGIFFRIT
                     TVITLVGGTIFLMWLGEQITARGVGNGISLIIFAGIVARLPHAVLGTLELGRTGSMST
                     GLILALIAMAVGVIAVIVFMERAQRRLLVQYPKRQVGNRMFQGDSSHLPLKLNSAGVI
                     PPIFASSLLLLPITFANFSSGQGPEWLTTVTTLLGQGQPLHLLIYVALIVFFAFFYTA
                     VVFNPKDTADNLKKYGGFLPGIRPGERTAEYIDYVLTRITVVGAIYLAGVCVLPEILV
                     SYAGAPFYFGGTSLLIVVSVTMDTVAQVQSHLLAHQYEGLIKRAKLRGARRQ"
     gene            complement(5414..5860)
                     /gene="rplO"
                     /locus_tag="VN518_00870"
                     /pseudo
     CDS             complement(5414..5860)
                     /gene="rplO"
                     /locus_tag="VN518_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012253650.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
     gene            complement(5866..6075)
                     /gene="rpmD"
                     /locus_tag="VN518_00875"
     CDS             complement(5866..6075)
                     /gene="rpmD"
                     /locus_tag="VN518_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003495212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HXE03156.1"
                     /translation="MAKTTDRKTIVVEQIGSPIRRPGIQRATLIGLGLNKMHKRRTLI
                     DSPQVRGMVAKIHHLVRIVDEEAGK"
     gene            complement(6107..6694)
                     /gene="rpsE"
                     /locus_tag="VN518_00880"
     CDS             complement(6107..6694)
                     /gene="rpsE"
                     /locus_tag="VN518_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012319173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HXE03157.1"
                     /translation="MADRKGGRGGERRDRDDRERDSEFIDKLVHINRVAKVVKGGRRF
                     GFAALVVVGDQKGRVGFGHGKAREVPEAIRKATEAAKRNLVKVPLREGRTLHHDVTGQ
                     HGAGRVILRAAPAGTGIIAGGPMRAVFETLGIQDVVAKSLGSSNPYNMVRATFDALRH
                     EDSPRGVAARRGKKVSEIVSRRRDGAGESAGQAEA"
     gene            complement(6708..7070)
                     /gene="rplR"
                     /locus_tag="VN518_00885"
     CDS             complement(6708..7070)
                     /gene="rplR"
                     /locus_tag="VN518_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018115512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HXE03158.1"
                     /translation="MSALNEGFLRRAHRVRHTLKRKAHGRARLSVFRSSKHIYAQVID
                     DNAGRTLVAASTLDKDLKGALKKGSDLEAAQAVGKLVAERALKAGVTDVVFDRGGYIY
                     HGRVKALADAAREAGLNF"
     gene            complement(7082..7615)
                     /gene="rplF"
                     /locus_tag="VN518_00890"
     CDS             complement(7082..7615)
                     /gene="rplF"
                     /locus_tag="VN518_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006205452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HXE03159.1"
                     /translation="MSRIGKKPVTIPKGVTASVNGQEVAVKGPKGELKHVLVDDVIAK
                     LEDGSIEVSPRQDTQNARAMWGMSRTLISNLMTGVTEGFTRKLEITGVGYRAAVAGSN
                     LQLQLGYSHDVIFPIPQGIQVVCPKPTEIVITGIDKQKVGQVAAEIRKFRPPEPYKGK
                     GVRYAGEYILRKEGKKK"
     gene            complement(7641..8039)
                     /gene="rpsH"
                     /locus_tag="VN518_00895"
     CDS             complement(7641..8039)
                     /gene="rpsH"
                     /locus_tag="VN518_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006936438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HXE03160.1"
                     /translation="MSINDPLGDMLARIRNAQMRHKAKVTTPASSLREHVLDVLVEEG
                     FIRGYARVDYTGGRSEFDIELKYFDGEPVIKDIKRISTPGRRVYSSVRDLPTVANGLG
                     VSILSTPKGVMSDSRARTENVGGEILCSVF"
     gene            complement(8048..8353)
                     /gene="rpsN"
                     /locus_tag="VN518_00900"
     CDS             complement(8048..8353)
                     /gene="rpsN"
                     /locus_tag="VN518_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013652496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HXE03161.1"
                     /translation="MAKTSMVERNNKRRRLAKKFKVRRTRLKAIAADRKLPAEERFAA
                     RLKLAELPRNSSPTRIRNRCDLTGRPRGYYRKLHMSRIALRDLASQGLIPGMIKSSW"
     gene            complement(8390..>8875)
                     /gene="rplE"
                     /locus_tag="VN518_00905"
     CDS             complement(8390..>8875)
                     /gene="rplE"
                     /locus_tag="VN518_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011975179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HXE03162.1"
                     /translation="EFGYKNPMQVPRLDKVVLNIGVGEGVADSKKVTAAAADLALIAG
                     QKPVITKARKSIATFKLREGMPIGAKVTLRQNRMYEFVDRLVNIALPRVRDFRGLNPK
                     SFDGRGNFTLGLKEHIIFPEINFDKVEEIWGMDIVVCTTAPTDDEARALLKHLNFPFR
                     S"
BASE COUNT         1510 a         2862 c         2740 g         1763 t
ORIGIN      
        1 ggcggcaaga tcaaggagaa gctcaccggc accgaggatc tcggttacag ctacgaaatc
       61 gtagagagcc ccctccccgt caagaactac aaatccaagc tctggctcga ggtcgatgac
      121 gagcccgatc gctcggtgat ctattggcaa tccgatttcg atgccaatgg cgcgagcgac
      181 gacgacgcca aggcgaagat caccggcatt ctcgccgacg gcgtgaaggg tatcaagcag
      241 caggcgatca ccgcccacga tgcgaaagaa gccgcggccg gcaagactcc ggaaggcgat
      301 aaggacaagg acgacgacga taagtagagt ctgagaaagg cgcctgatcc ggcgcgtggt
      361 tttatcgccc ggccgttctc aatcaggacg gccgggtttt ttgtcagccg cccattgaaa
      421 actggactta gggaaccgct cgccgcttac gcggccttct cggcctcggc gtcacgctct
      481 tgcaccggcc ctgaatcgag gccgcgtgcg gcttcatcgc gatccacgag ctcgatcacg
      541 gccatgggcg cagagtcgcc gtagcggaag cctgccttca tcacccgcgt atagccgcct
      601 gacctgtcgg catagcgtgg gccaagcgtg ccgaacagct tcgccaccat ggcctcatcg
      661 cgaatgcgcg acatggcaag gcggcgcgca tgcagatcgc ctttcttggc gagggtgatc
      721 agccggtcgg ccacgcggcg aaggtccttc gccttgggca aggtggtcac gatctgctcg
      781 tgcttgatga gcgcgcacgc gaggctcgag aacagggccg agcgatgact ggcagtacga
      841 ttgagcttcc ggccactgtt gcggtgacgc atttcaaaac ccctggcttc taactttccg
      901 gctgctggct aataatgatc ttcgaagcgc ttggcgagct cttcgatgtt ctccggcggc
      961 cagtccggta cttccatgcc gagatgcagg cccatggagg cgagcacttc cttgatctcg
     1021 ttcagcgact tacggccgaa attcggggtg cgcagcatct cggcttccgt cttctggatg
     1081 aggtcgccga tatagacgat gttgtcgttc ttcaggcaat tcgccgagcg caccgacagc
     1141 tcgagctcgt cgaccttctt gagcagcgcc gcgttgaaca cgagctcagg ctgccgctcg
     1201 aagggcatct ccttcttcgg ctcctcgaaa ttgacgaaca ccgagagctg gtcctgcacg
     1261 atgcgcgcgg ccaaagccac ggtgtcctcc ggccgcaccg agccgttggt ctcgatctcc
     1321 atggtgagct tgtcgtagtc gaggatctgg ccttcgcggg tgttctccac gcgataggag
     1381 accttgcgca ccgggctata gagcgagtcg accggaatga agccgattgg cgcgtcgtcg
     1441 ggacggttgc gctcggccgg aacgtagccc ttgccagtcg aggtggtgaa ctccatgcgg
     1501 atcgcggcgc cctggtcgag ggtgcaaatc acatgatccg gattgaggat ctcgatgtcg
     1561 gcgccggtct cgatatcgcc ggccttggcc acgccgggtc cttccttctt cagcaccatg
     1621 cgcttcacgc cctcggcatg ctggcggagc gcgatctcct tgatgttgag gatgatgtcg
     1681 gtgacatcct cgcgcacacc ggcgatcgag gagaactcgt gcagcacgcc gtcgatatgc
     1741 actgaggtga tcgccgcgcc ctgaagcgag gagagcaaca cgcggcgaag cgcgttgccg
     1801 agggtaagcc cgaacccgcg ctcgagcggc tcagccacga cgagcgcgaa acgcgccgga
     1861 tcgcggccgg acgagacgtt gagcttggtg ggcttgatga gatcttgcca gttcttctgc
     1921 agcacctgga gggccctttc ctggtcctta agcggggccg cctggcccag cttgcgagaa
     1981 tccgcgtgta acatgaggac ttttgccttc ccttagaccc gccgccgctt gcgcgggcgg
     2041 cagccgttat gcgggatcgg cgttacatcg cggatggtga tgatggtgaa gcccgcggct
     2101 tgcagcgccc gaagcgccga ttcacggccc gagcctggcc cgcgaacctc gatttcgaga
     2161 ttcttcatgc cgtgctccat ggccttcttg ccggcgtcct cggcagcgac ctgcgccgca
     2221 tagggcgtgg atttgcgcga tcccttgaaa cccatggccc ccgccgacga ccaggagatg
     2281 gtgtttccct gcgcatcggt gatggtgatc atggtgttgt tgaaacttgc atgcacatga
     2341 gcgacgcccg aggtgatgtt tttcttttcc cggcggcgaa cgcgtgtctt gtctttggcc
     2401 atgctttatt cccgcatctt cacaaattgg ctccggccga agatcacgct tcagccaagc
     2461 tcgattgaaa ctcttacttc ttcttgcctg cgatcggctt ggctggacct ttgcgcgtgc
     2521 gcgcattggt gtgggtcctc tggccatgca ccggcaagcc gcgtcgatgc ctcaagccgc
     2581 gataacagcc aagatccatc aatcgcttga tattcattgc gacatcgcgc cgcaaatcgc
     2641 cctcgaccat gtagtcccgg tcgatcacct cgcgaatctg aatgacctcg gattcggtga
     2701 gctggctgac ccgccgctca ggtggaatgc ccactttggc taggatctgc ttggcaaagg
     2761 tatcgccaat gccatggata taggtgagcg ccacctgcac ccgcttgttg gacgggatat
     2821 ttacacctgc aattcgggcc acgaagcttt ctcctcgaag cacgccttaa cgtgctgaaa
     2881 tttaatggca attcgcaatg gaacgcagca aaaccgacct cgggaaggca ccgccccccg
     2941 ggaccggtcg ttcctatcca gaaatgggtc gtgccttatc tagcgattcc gcttgacaag
     3001 gtcaaccggg ggtcttggcg gatttaagct gacccatcca aggcccgccc gatggcccgg
     3061 cgcacctcac cgatttcagc catgccatcg accgatttga gctttccctg tttcgcataa
     3121 tagttgatca gaggcttggt ctgggcgtga taggcctcga gccttgaccg gaccacctcc
     3181 tcattgtcgt cgcgccgccg cacgaattcc ctcgagccgc agcggtcgca gacgccggga
     3241 accttcggcg tcttgaagtc gtcgtgatag ccctcgccgc aattggcgca agtgaagcgg
     3301 ccgatgatgc gcttgaccag ggcatcgtcg tcgacctgca tctcgaccac cgcgtcgagc
     3361 gtcttgcccc gctccttaag cagccggtcg agcgcctcgg cctgggcgat ggtccgggga
     3421 aatccgtcga ggatgaagcc ctcaccctct ccgcctctat ccagccgctc ggcgatcaac
     3481 ccgatcacga tgtcgtcggg cacgagcgcg ccccgttcca tgatgtcgcc ggccttgcgg
     3541 ccaagctcgg tcccggccgc cacggccgcc cgcaacatgt ctccggtgga aagcttgacg
     3601 agcgaacggt cggtctccag cagattggcc tgggtcccct tgccggcacc gggaggacca
     3661 agcaggacca ggatcattgc cgtcgcgctc ccctgagctt ggcccgcttg atcagccctt
     3721 catattgatg ggccaagaga tggctctgca cctgggccac ggtgtccatg gtgacgctca
     3781 ccacgatgag gagcgaggtg ccgccgaaat agaacggcgc gccggcgtaa gagacgagga
     3841 tctcagggag cacgcagacg ccggcgagat agatggctcc caccacggtg atgcgggtga
     3901 gcacgtagtc gatatattcg gcggttcgct cccctggcct gatgccgggc agaaagccgc
     3961 catacttctt gagattgtcg gcggtgtcct tcgggttgaa cacgacggcc gtgtagaaga
     4021 aggcgaagaa gacgattagc gcgacataga tgaggaggtg cagaggctgg ccctgcccga
     4081 gcagggtggt gaccgtggtc agccattcag ggccttggcc cgaggagaaa ttggcgaagg
     4141 tgatgggcaa caacaggagc gaggaggcga agatcggcgg gatcacgccg gcgctgttga
     4201 gcttgagcgg gaggtgggag gaatcgccct gaaacatgcg gttcccgacc tggcgcttgg
     4261 gatattgcac cagcagtcgc cgctgcgccc gctccatgaa cacgatcacg gcgatcacgc
     4321 ccacggccat ggcgatgagc gcgaggatca gcccggtcga catcgagccg gtgcggccaa
     4381 gctcgagggt gccgagcacc gcgtgcggca gacgcgccac gataccggcg aagatgatga
     4441 gcgagatgcc gttgcccacg cccctcgccg tgatctgctc gccaagccac atcaggaaga
     4501 tggtgccccc gaccagcgtg atcaccgtgg tgatgcggaa gaatatccca gggtcgagca
     4561 ccacattgcc ggcgccttcc agccccacgg cgatcccata ggcctgcagc gccgcgagca
     4621 gcaccgtgcc gtagcgcgtg tactggttca tctgccggcg tccctgctcg ccctccttct
     4681 tcaactgctc aagatgcggg gacaccgtgg tgagcagctg gataatgatc gaggccgaga
     4741 tgtacggcat gatgttcagc gcgaagatcg ccatgcggcc gacggcgccg ccggaaaaca
     4801 tgtcgaacat gccgagcaag cccgactggt tctgccggaa gacttcggcc aacgccgtcg
     4861 ggttgatgcc ggggatcggg atataggttc cgagccggta gatgaggagc gcgccgaggg
     4921 tgaaccagat gcgcttcttg agctcgtccg ccttggcgaa ggcggcgaag ttcagattgg
     4981 aaaccagctg ttcggcagca gaaaccatgg ccgagctctc ttccgttacg acactatcga
     5041 gcgaggtacg cggccgctcg cggcatttct agggcacggc caggacaacc aattgtgaca
     5101 ggccgctctg gcaagcccgt taggacttgt ccttcttccc ggcttgaggc ttcggctgct
     5161 tcttgtcctc gccctcggcg gtcttttcct tctttggcgc cttggcttgg gcctctgcag
     5221 ccggctcacc cttggccttg gcagccttgg tcggctttgc ctccccgggc tcagcttgcg
     5281 cctcgccctc ggccttaggc gccttgccgg acttcttcgc cttcgctgcc gaggccgcct
     5341 cgcgcttctc ggcgcgcttc ttggccttga tcgcgtcggc cgctggcgcc tcgcggccgg
     5401 tgatggattt cagcgtcacc gtgccgccgg cagcttccac cgccttgatc gcgctgcctg
     5461 aggcgccggt cacctcgaag gcgagcttgc tcttgagctc accgtggccg agcaggcgca
     5521 cgccgtcctt ggtgcgccgg atgaggcctg cttgcttcag cgcatcgacg gtgatcggct
     5581 gcttcgcatc gagcaggcca gcatcgatcg ccgactgaac gcgcccgaga ttgagctcgt
     5641 tgtacttctt ggcgaagatg ttgttgaagc cgcgcttcgg cagacgccgg tggagcggca
     5701 tctggccgcc ctcgaagccc ttgatggcga cgccggtgcg agccttctgg cccttgacgc
     5761 cgcgccctga cgtcttgccg aggcccgagc cgatgccgcg gccaagccgc ttcttggctt
     5821 tggtggcgcc gtcccggtcc ctgatctcat tcagccgcat gatctctact ttcctgcctc
     5881 ctcgtccacg atgcggacga gatgatgaat cttcgcgacc atgccgcgca cttgcggact
     5941 gtcgatcagc gtccggcgct tatgcatctt gttgagcccg agcccgatca aggtggcgcg
     6001 ctgaatgccg gggcgcctga tcgggctgcc gatctgctcg accacgatcg tctttctgtc
     6061 ggtcgtcttg gccatgtttc gttacctttc ggcgcttccc gcagacctaa gcctcggcct
     6121 ggccggcgct ctcgccggcg ccgtctctgc ggcgcgacac gatctcgctc accttcttgc
     6181 cgcgccgggc cgctacgccg cgcgggctgt cctcatggcg gagcgcatcg aaagtggcgc
     6241 gcaccatgtt gtaagggttg gagctgccga gcgacttcgc caccacgtcc tggatgccga
     6301 gggtctcgaa caccgcgcgc atcgggccgc cggcaatgat gccggtacct gccggcgccg
     6361 cgcgtaggat cacgcggcca gcgccatgct gtccggtcac gtcgtgatgc agcgtgcgcc
     6421 cttcgcgaag cggcaccttg accaggttcc gcttggcagc ctcggtcgcc ttgcggatcg
     6481 cctccggcac ctcgcgcgcc ttgccgtggc cgaagccgac gcggcccttc tggtcgccga
     6541 cgacgacgag cgcggcgaag ccgaaacgcc ttccgccctt caccaccttg gccacgcgat
     6601 tgatgtgcac gagcttgtcg atgaactcgc tgtcgcgctc gcgatcatcg cggtcccgtc
     6661 gctccccgcc tctgccgcct ttacgatctg ccatgctctt cggtccttta gaaattcagc
     6721 cctgcctcgc gggcagcatc ggcgagcgcc ttgacgcgcc catggtagat atagccgccg
     6781 cggtcgaaca cgacgtcggt gacgcccgct ttcagcgccc gctcggcgac gagcttgccg
     6841 acggcttgcg ccgcttcgag gtcgctgccc ttcttgagcg cgcccttcag atccttgtcc
     6901 aaggtcgatg ccgcgaccag ggtgcggccg gcattgtcgt cgatcacctg ggcgtagatg
     6961 tgcttggagg agcggaacac cgacaggcgc gcacgtccat gcgccttacg cttgagcgta
     7021 tggcgcacgc gatgtgcacg ccgcaaaaat ccttcgttca aagcgctcat gaccgatccc
     7081 gctacttctt cttgccctct ttacggagga tgtactcgcc cgcatagcgg acgcccttgc
     7141 ccttataggg ctccggcggc cggaactttc tgatctcagc cgccacctgc cccaccttct
     7201 gcttgtcgat gccggtgatc acgatctcgg tcggcttcgg gcacaccacc tggatgcctt
     7261 gcggaatggg gaagatgacg tcgtggctgt agccgagctg gagctggagg ttcgatccag
     7321 ccaccgcggc ccggtagccc acgccggtga tctcgagctt cctggtgaag ccctcggtca
     7381 cgccggtcat gaggttcgag atcagcgtcc gcgacatgcc ccacatggca cgcgcgttct
     7441 gcgtatcctg ccgagggctg acctcgatgc tgccgtcctc gagcttggcg atgacgtcgt
     7501 ccacgaggac gtgcttgagc tcgcccttcg ggcccttcac cgccacctcc tggccattca
     7561 ccgaggcggt tacgcccttc ggaatggtca ccggcttttt gccaatacgc gacatcgaaa
     7621 tcgcccttcg tccgtaagtc ctagaacacg ctgcacagaa tttcgccgcc cacattttcg
     7681 gtgcgggcac gggagtcaga catgacgcct ttcggcgtcg aaagtatgga gacgccaagc
     7741 ccgttggcga cggtcggcag atcgcgcacc gaggaataca cccgccgccc cggcgtcgag
     7801 atgcgcttga tatccttgat taccggctcg ccgtcgaaat atttaagctc gatgtcgaac
     7861 tcggaccggc cgccggtgta atcgacacgc gcatagccgc ggatgaagcc ctcctcaacc
     7921 agcacgtcga gcacatgctc gcgcaagctg gaggcgggcg tcgtcacctt agccttgtgg
     7981 cgcatctgcg cgttacggat gcgcgcgagc atgtcgccta ggggatcgtt gatgctcatc
     8041 agtggtccta ccagctcgac ttgatcattc cgggaatcag tccttggctc gcgagatcgc
     8101 gcagcgcgat gcgcgacatg tggagcttgc ggtaataacc gcgcggcctc ccggtgaggt
     8161 cgcagcggtt gcggatacgc gtcggcgagg agttgcgcgg cagctcggcg agcttgagcc
     8221 gcgccgcgaa gcgttcctcg gccggaagct tgcggtcggc ggcgatggct ttcagccgcg
     8281 tccgccgcac cttgaatttc ttggccagcc ggcggcgctt attgttccgc tcgaccatgc
     8341 tcgtctttgc cattcagtcc tcctaacgca gcgtcaagcc gccatgccct tagctgcgga
     8401 acggaaagtt caaatgcttc agcaacgcac gcgcctcgtc gtcggtaggc gccgtggtgc
     8461 atacaacgat gtccatgccc cagatctcct cgaccttgtc gaagttgatc tcggggaaaa
     8521 tgatgtgctc cttgagcccg agggtgaaat taccgcggcc gtcaaagctc ttcgggttca
     8581 gccctctgaa gtcgcgcacc cgaggcagcg cgatgttaac gaggcgatcg acgaactcgt
     8641 acatccggtt ctggcgcagc gtcaccttgg cgccgatcgg catgccctcg cgcagcttga
     8701 aggtggcgat cgacttgcgc gccttggtga tcaccggctt ctgcccggcg atgagcgcaa
     8761 gatcggcggc ggcggcggtc accttcttgg aatcggcgac gccttcgccg acgcctatat
     8821 tcagcacgac cttgtcgagc cgcggcacct gcatcgggtt cttgtagccg aactc
//
LOCUS       DATNAE010000048         2096 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_349584, whole genome shotgun sequence.
ACCESSION   DATNAE010000048 DATNAE010000000
VERSION     DATNAE010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349584"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VN518_00910"
     CDS             complement(<1..287)
                     /locus_tag="VN518_00910"
                     /inference="COORDINATES: protein motif:HMM:NF040248.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXE03163.1"
                     /translation="MTRFILTRLAQVLAVLAVMSFIIYGLIGLMPGDPIDLMLTADPH
                     LTAADAARLKSLYGLDRPLSERYVAWSEAALKGDFGYSRLYGTPAIKALAPR"
     gene            complement(284..1954)
                     /locus_tag="VN518_00915"
     CDS             complement(284..1954)
                     /locus_tag="VN518_00915"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HXE03164.1"
                     /translation="MRRLLLALLSLLPLIASPAIAGEAPDELAIGVTQFPSTLHPSID
                     AMVAKTYVLGMVRRPLTTYDADWQLVCMLCVTLPSIENGLAQVLDLGDGKRGIRLTYT
                     IRPDAVWGDGVPVTTDDVKFTYEVGRNPQSGVGDAELYRRITKIEAKDAKTFTMDVDK
                     LTFDYAGINDFEILPAHLERAAFADPAQYRFRTLYDTDPTNPGLYFGPYRITEVSPDS
                     HIVLERNPTWWGKPPAFRRIVLWTVENTAALEANLLAGGLDMVAGELGFSLDQALGFE
                     KRHGDAFTVLYKPSLSYEHIDLNLDDPILRDQRVRQALLYGVDRKAISTQLFAGRQPV
                     AESFVSPLDWVYTTDVRHYPYDPAKAAALLDDAGWRRDGDGIRRNAQGEPLALTLGTT
                     AGNRIRELIEQVLQSQWRQLGIELRLKNQPARVFFGETVTKRKFAMALFAWTSAPENV
                     PRSTLRSDEIPTADKGYRGQNYTGFHDDEADRLIDAIEIELDRDKRAALWHRLEALYA
                     EKLPALPLFFRADAYVLPKWLKGVTPTGHQYPTTLWIEDWRVEGRVTG"
BASE COUNT          372 a          650 c          731 g          343 t
ORIGIN      
        1 cgcggcgcca gcgccttgat ggcgggcgtg ccatagaggc gcgaatagcc gaaatcgccc
       61 ttgagcgccg cctccgacca ggcgacatag cgctcgctca agggccggtc gaggccgtaa
      121 agcgacttca gccgcgccgc atcggccgcc gtcagatgcg ggtcggcggt cagcatcaga
      181 tcgatgggat cgcccggcat gagaccgatg agcccataga tgatgaagga catgacggcg
      241 agcaccgcca gcacttgggc gaggcgcgtg aggatgaagc gggtcatccc gtcacgcgcc
      301 cctcgacgcg ccaatcctcg atccacaacg tcgtcggata ctggtggccg gtcggtgtca
      361 cgcccttgag ccatttcggc aggacatagg cgtcggcgcg gaagaagagg ggcagcgccg
      421 gcagcttttc cgcatagagc gcttcgaggc gatgccagag cgcggcgcgc ttgtcgcggt
      481 ccagctcgat ctcgatggca tcgatgagcc gatcggcctc gtcgtcatgg aatcccgtgt
      541 agttctgccc ccgatagccc ttgtccgccg tcgggatctc gtcggagcgc aaggtcgagc
      601 gcggcacgtt ctcgggcgcg ctggtccagg cgaaaagcgc catcgcgaat ttgcgcttgg
      661 tcaccgtctc gccgaagaag acgcgcgccg gctggttctt gaggcgaagc tcgatgccga
      721 gctgccgcca ttggctctgc aggacttgct cgatcaattc gcggatgcga ttgccggcgg
      781 tggtgccgag ggtgagcgcc agaggctcgc cttgcgcgtt gcggcggatg ccgtcgccat
      841 cccggcgcca gcccgcgtcg tcgagcagcg ccgccgcctt ggccgggtca tagggatagt
      901 gccggacgtc ggtcgtatag acccaatcca agggcgagac gaagctctcg gcgaccggct
      961 ggcggccggc gaagagctgc gtgctgatcg ccttgcggtc gacgccatag aggagcgcct
     1021 ggcgcacgcg ctgatcccgg aggatgggat cgtcgagatt gagatcgata tgctcatagc
     1081 tgaggctcgg cttgtagagc acggtgaagg catcgccatg gcgcttctcg aagcccagcg
     1141 cctggtcgag ggaaaagccc agctcgcccg ccaccatgtc gagcccgccg gccagcagat
     1201 tggcctcgag cgccgcggtg ttctcgaccg tccagagcac gatgcggcgg aacgctggcg
     1261 gcttgcccca ccaggtggga ttgcgctcga gcacgatatg cgagtccggc gagacctcgg
     1321 tgatgcggta gggcccgaaa tagagcccgg ggttggtcgg atcggtgtcg tagagcgtgc
     1381 ggaagcggta ctgcgcggga tcggcgaagg cagcgcgctc gagatgcgcc ggcaggattt
     1441 cgaaatcatt gatgccggca taatcgaagg tgagcttgtc gacatccatg gtgaaggtct
     1501 tggcgtcctt cgcctcgatc ttggtgatgc ggcggtaaag ctcggcatcg ccgacgccgc
     1561 tttgcgggtt gcgcccgacc tcgtaggtga atttgacgtc atccgtggtg accggcacgc
     1621 catcgcccca gacggcgtcg ggccggatcg tgtaggtcaa tcgaatgccg cgcttgccgt
     1681 cccccagatc gagcacctgc gccaggccat tctcgatgct cggcagcgtc acgcacagca
     1741 tgcagacgag ctgccagtcg gcgtcatagg tggtgagcgg ccggcgcacc atgcccagca
     1801 cataggtctt cgccaccatc gcatcgatcg agggatggag cgtcgaagga aactgcgtga
     1861 cgccaatggc gagctcgtcc ggcgcttcgc ccgcgatggc gggcgaggcg atgagcggca
     1921 agagcgagag aagcgccagg agcagacgcc gcatcacacc tccgacgtca ttgcgagcaa
     1981 aagcgaagca atccagcggg aagcaacctc ggcggtggat tgccgcgtcg gcctagggcc
     2041 gcctcgcaat gactgcatcg gggcaagata gcacggcgcc gcgccgcgtc gattgc
//
LOCUS       DATNAE010000049         8278 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_1856991, whole genome shotgun sequence.
ACCESSION   DATNAE010000049 DATNAE010000000
VERSION     DATNAE010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 8278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8278
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1856991"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            28..885
                     /locus_tag="VN518_00920"
     CDS             28..885
                     /locus_tag="VN518_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HXE03165.1"
                     /translation="MGGLLPPAWTGRGALIPVLRFSGAIGLSTPLRPGLTLSTSATAI
                     EKAFSMRGAKAVAIQINSPGGSAVQSVLIYKRIRALAEEKGLMVYVFAEDVAASGGYL
                     LAIAGDEIYADASSIVGSIGVVTATFGLNELINKIGVERRVYTAGRNKTTLDPFLPVK
                     EEDVERVKAIQRDVHAEFIDLVKARRGVRIEKAGDDLFTGEFWSGRKALELGLIDGLT
                     DLRTKMRELYGDEVRFRLVTPSTSWLRRRRSVAIGDSASNFGFSPGGLAADLISAIEA
                     RALWSRFGF"
     gene            888..1082
                     /locus_tag="VN518_00925"
     CDS             888..1082
                     /locus_tag="VN518_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03166.1"
                     /translation="MPQLLILLAAGAGIFLARRWYRDEQRRVAAELARARKAMDQHEA
                     GSVVRLERDPATGVYSPKPH"
     gene            1185..2117
                     /locus_tag="VN518_00930"
     CDS             1185..2117
                     /locus_tag="VN518_00930"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HXE03167.1"
                     /translation="MGKLTKADRPHAAPSFQDLILKLQRYWADQACVILQPYDMEMGA
                     GTFHPATTLRALGPKPWRAAYVQPSRRPKDGRYGENPNRLQHYYQFQVILKPSPPDIQ
                     DLYLDSLRAIGVDVANHDIRFVEDDWESPTLGAWGLGWEVWCDGMEVSQFTYFQQVGG
                     FDCDPVSGELTYGLERLAMYVQGVDRVFDLNFNGGRGKDKVTYGDVFLQSEQEYSRYN
                     FEQADTKMLLAHFRDAEAECKALLAKGDDGKRHLMVLPAYDQAIKASHIFNLLDARGV
                     ISVTERQAFILRVRELARAVCAAWLKTEAGGAHA"
     gene            2132..4195
                     /gene="glyS"
                     /locus_tag="VN518_00935"
     CDS             2132..4195
                     /gene="glyS"
                     /locus_tag="VN518_00935"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015823336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HXE03168.1"
                     /translation="MAELLIELSSEEIPARMQTRAAEDLERLIGDRLKDAGLTFGTVR
                     SFATPRRLAAVVDGLPTRSPDVSEEKKGPRVGAPEAAVQGFLKSAGLMSLDQAEIRAD
                     KKGDFYVATIKRSGRATSQVVAEIVPEIVRNFPWPKSMRWGNGRLRWVRPLHSILCIF
                     GGKVVPFEIDGIKSGKETRGHRFMAPKPFSVRSFDDYVQRLRNAFVILDPREREAIIL
                     KGAQKLAAKQQLLLVEDIALLAENAGLTEWPVVLMGSFDASFLDVPGEVLATSMKAHQ
                     KCFSLRRGERLANRFVMVANLEAKDRGKAIVAGNERVIAARLADAKFFFDHDRKVMLE
                     ERVPKLREIVFHEKLGTQYERVQRVRVLAREVAKLVRADPDLAERAAILAKADLISDM
                     VGEFPELQGVMGRYYALDQEEDALVANAIGAHYKPVGPSDDVPRDPIAISVALADKLD
                     MLTGFWAIDEKPTGSKDPYALRRAALGIIRIVLENDLRLPLNDVTRAALQKWRQLTPR
                     GSADVQASLLAFFADRLKVHLRDQGARHDLVDAVFALGGDDLLTIVRRVEALGLFLDT
                     EDGEHLLTGTKRAINILRIEEKKDSCSYDQPPDASLLKQPEELALAKTVDEVERAAAA
                     AVGKEDFEAAMAAMARLRAPVDAFFDHVTVNTDDPALRENRLRLLNRIRSTTLTVADF
                     SKIEG"
     gene            4201..4629
                     /locus_tag="VN518_00940"
     CDS             4201..4629
                     /locus_tag="VN518_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXE03169.1"
                     /translation="MPAHVSLITLGVADVAKATSFYERLGFLRSKKPSEASVSFFQAG
                     PVVLALWGRDAQRDDAQAAELWTGNGGIVVAQNLASEDAVDTMMAKAEAAGARILKPA
                     AKTFWGGYDGYFADPDGHVWEVAFNPFWELDEDGRVRLPE"
     gene            4634..5053
                     /locus_tag="VN518_00945"
     CDS             4634..5053
                     /locus_tag="VN518_00945"
                     /inference="COORDINATES: protein motif:HMM:NF017868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HXE03170.1"
                     /translation="MTDYLFVYGTLRSEFPHPLARRLQAQAKFIGKGSTPGALYDFGW
                     YPGARFDPHARTHVIGEVFSIKNAERLLAELDHYEGTAEPGNAFRRVPVTVRLDHGGT
                     VDAWSYEMGDSKARRRPIESGDFIHHRRLKDQRQVRG"
     gene            5117..7819
                     /gene="ppdK"
                     /locus_tag="VN518_00950"
     CDS             5117..7819
                     /gene="ppdK"
                     /locus_tag="VN518_00950"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HXE03171.1"
                     /translation="MKPAAKAKDPSPHWVFTFGAGRAEGDAAQRDLLGGKGAYLAEMS
                     RLGLPVPPGLTISSEVCTAYYRSGKMLPAALMPMVSEALDVVGKVAGARFGNVDNPLL
                     VSVRSGSRASMPGMMDTVLNLGLNDETVEGLARRSSDRRFAYDTYRRFIQMYGDVVLG
                     IDHYLFEEVLENYKSLKGFELDSELGQEHWAELVARFKALVEQELGQPFPQDLHVQLW
                     GAIAAVFGSWHNARAIAYRRLHDIPDDWGTAVTVQAMVFGNMGERSATGVVFTRNPST
                     GENALYGEFLVNAQGEDVVAGLRTPQPLTEAARNATGDRRPSLEALMPETFADLKTAC
                     AKLEAHFRDIQDIEFTVEEGRLWMLQSRSGKRSMQAALKIAVDMVGEGLITEKEAVLG
                     IDAAQIDQLLHPTLDPDSPKLVIGKGLPASPGAASGKLVFDADEAVHLKAQGHTLILA
                     RVETSPEDVQGMHAAAGIITTRGGMTSHAAVVARGMGRPCVVGAASVQIDLERETLSA
                     GGETLGKGDVVTIDGSTGQIIKGRVPTREPTLSSDFLTLMGWADQFRRMKVRANADTP
                     TDARQSLAFGADGIGLCRTEHMFFEEGRIVPMREMILADDTEGRRAALAKLLPMQRKD
                     FIELFEIMAGAPVTIRLLDPPLHEFLPHGEAAIGEVAAAMGVSAARLKRRVAGLSEFN
                     PMLGHRGSRLLVSFPEIAEMQARAIFEAAIEAGRKLGTTVVPEIMVPLVTAKAELDLV
                     KERIDGVARAVEKEQGVKLTYSVGTMVELPRACINAGEIGKSADFFSFGTNDLTQTTF
                     GLSRDDAGRFLSEYTEKGIIQHDPFVTLDPAVGELMWIAVERGRRSHPDLKIGICGEH
                     GGDPETIRFCEDIYLDYVSSSPYRVPVARLAAAQAALSLTVKPR"
BASE COUNT         1463 a         2691 c         2652 g         1472 t
ORIGIN      
        1 gagcttcgcc cttctctgtt ggaccgggtg ggaggtctcc tgccgccggc ttggaccggg
       61 cgcggcgcgc tgatcccggt gctcagattc agcggcgcca tcggcttgtc gaccccgctc
      121 cgcccgggcc tcacgctttc caccagcgcc accgcgatcg aaaaggcttt ctccatgcgc
      181 ggggccaaag cggtcgccat ccagatcaac tctccaggcg gctccgccgt acagtcggta
      241 ctgatctaca agcgcatccg cgcgcttgcc gaggagaagg gtctgatggt ctatgtgttc
      301 gccgaggacg tcgccgcgtc tggaggctac ctgcttgcga tcgccggcga cgagatctac
      361 gccgatgcaa gctcgatcgt cggctcgatc ggcgtcgtca ccgccacgtt cggtctgaac
      421 gagctgatca acaagattgg cgtcgagcgg cgcgtctata ccgccggccg caacaagacg
      481 acgctagacc cgttcctgcc ggtgaaggag gaagacgtgg agcgggtcaa ggccatccag
      541 cgcgacgtgc atgctgagtt catcgatctc gtcaaggcga ggcgtggcgt gcgcatcgag
      601 aaggctggag acgacctgtt caccggcgag ttctggtccg gcaggaaggc gctcgagctc
      661 gggctgatcg acggcttgac cgacctccgc accaagatgc gcgagctcta cggcgacgaa
      721 gtccgcttca ggctggtcac gccgtcgacg agctggctcc gccgcaggcg cagcgtcgcc
      781 ataggcgaca gcgcttccaa cttcggcttc tcgcccggcg ggcttgcagc cgacctcatt
      841 tctgctatcg aagcgagagc gctctggtcg cgctttgggt tctagatatg ccgcaacttc
      901 tcattctgct ggccgcaggt gccggcatct tcctggcaag acgctggtat cgcgacgagc
      961 agcgccgcgt cgccgccgag cttgcccggg cgcgcaaggc catggatcag cacgaggcag
     1021 gcagcgtcgt tcgtctggag cgagacccgg cgaccggcgt ctacagcccc aagccccact
     1081 gatcagccgc ctgatcaaga cgcgcctccc ttgacccacc cccgcgcccg cgcatagcgt
     1141 ctggcgcctc gtcgacgtcc cctctccccg cctggaaagc gaccatgggc aagctcacca
     1201 aagcggaccg gcctcacgcc gccccctcgt tccaggatct gatcctgaag ctccagcgct
     1261 attgggccga tcaggcttgc gtcattttgc agccctacga catggagatg ggggccggta
     1321 cctttcatcc cgccacgacc ttgcgcgcgc ttgggccgaa gccctggcgc gcggcctacg
     1381 tccagccctc tcgaaggcct aaggacggcc gctacggcga aaacccgaac cggctgcagc
     1441 attactacca gttccaggtg atcctgaagc cgtcgccgcc agacatccag gacctctatc
     1501 tcgactccct ccgcgccatc ggcgtcgatg tcgccaatca cgatatccgc ttcgtcgagg
     1561 acgactggga gagcccgacg ctcggcgcct gggggttagg ctgggaagtg tggtgcgacg
     1621 gcatggaggt gtcgcagttc acctacttcc agcaggttgg cggcttcgat tgcgaccccg
     1681 tctcgggcga gctcacctac ggtcttgagc ggctcgccat gtatgtgcag ggcgtcgacc
     1741 gcgtcttcga tctcaacttc aacggcggcc ggggcaagga caaggtcact tatggcgacg
     1801 tgttcctgca gtccgagcag gaatattccc gctacaattt cgagcaggcc gacaccaaga
     1861 tgctgctcgc ccatttccgc gacgcagagg ccgagtgcaa ggcgctgctt gccaaaggcg
     1921 acgacggcaa gcgacatttg atggtgcttc cggcctacga ccaggcgatc aaggcctcac
     1981 atatcttcaa tctgctcgac gcccgcggcg tgatctcggt gaccgagcgg caagccttca
     2041 tcctccgcgt gcgcgagctc gcccgcgcag tttgcgcggc ctggctcaag accgaagctg
     2101 gcggcgccca tgcttgatat gcggggcgct catggctgag cttttgatcg agctgtcctc
     2161 cgaggagatc ccggcgcgca tgcagacgcg cgccgccgag gatcttgagc gcctcattgg
     2221 cgatagactg aaagatgccg gactgacgtt tggcacggtg cggagcttcg ccacgccgcg
     2281 gcgtctcgcg gcagtggtcg atggcctgcc gacgcggtct ccagatgtaa gcgaggagaa
     2341 gaagggcccg cgcgttggcg cacccgaggc ggcagtgcaa ggattcctca aatctgccgg
     2401 gctcatgtct ctcgaccagg ccgaaatccg cgccgacaag aagggcgatt tctatgtggc
     2461 gacgatcaag cgatctgggc gagcaacctc gcaagtcgtc gccgagatcg tgcccgagat
     2521 cgtgcgcaac ttcccctggc cgaaatcgat gcgctgggga aacgggcgct tgcgctgggt
     2581 gcggccgctg cattcgatcc tgtgcatctt cggcggcaag gtggtgccgt tcgagatcga
     2641 cggcatcaaa agcggcaagg aaacgcgcgg ccatcgcttc atggcgccga agccgttcag
     2701 tgtgaggagc ttcgacgatt atgtgcagag gctgcgcaac gctttcgtca tcctcgatcc
     2761 ccgcgagcgc gaggcgatca tcctgaaggg cgcgcagaag ctcgccgcga agcaacagct
     2821 cctgctggtc gaggacatag cgctgctcgc cgagaatgcc gggctcaccg aatggcccgt
     2881 ggtgctgatg ggcagcttcg atgcgagctt cctcgacgtg ccgggtgagg tcctcgccac
     2941 ttcgatgaag gcgcatcaga aatgcttctc gcttcgccgc ggcgagcgtc tcgccaaccg
     3001 cttcgtcatg gtggcgaacc tcgaggcaaa ggaccgcggc aaggccatcg tcgccggcaa
     3061 tgagcgggtg atcgcggcaa ggctcgccga cgccaaattc ttcttcgatc acgaccgcaa
     3121 ggtgatgctc gaagagcgcg tgccgaagct ccgggagatc gtgttccatg agaagctcgg
     3181 cacgcaatat gagcgggtgc agagggtgcg cgtgcttgcc cgcgaggtcg ccaagctcgt
     3241 ccgcgccgat cccgacctcg ccgagcgcgc cgcgatcctc gccaaggccg atctcatcag
     3301 cgacatggtt ggagagttcc ccgagctgca gggcgtgatg ggccgctatt acgcgctcga
     3361 ccaggaagag gatgcgctgg tcgcaaacgc gattggtgct cattacaagc cggtggggcc
     3421 gtccgatgac gtgccgcgcg atcccatcgc catctccgtg gcgctcgccg acaagctcga
     3481 catgctgacg ggcttttggg ccatcgacga gaagccgacc ggctcgaagg atccatacgc
     3541 gcttcgccgc gccgcgctcg gaatcatccg catcgtgctc gagaatgacc ttcgcttgcc
     3601 gctgaacgat gtgacccgcg ccgccttgca gaagtggcgg caactgaccc cgcgtggcag
     3661 cgccgacgtg caagcgagcc tcctcgcctt cttcgccgac cggctcaagg tgcatctgcg
     3721 tgaccagggc gcgcggcacg atctcgtcga tgccgtgttc gcgcttggcg gcgacgattt
     3781 gctcacgatc gtgcgccggg tggaggcgct tgggctcttc ctcgacaccg aggatggcga
     3841 gcacctgctc accggcacca agcgcgccat caacatcctg cgcatcgagg agaagaaaga
     3901 ttcttgcagc tacgatcagc cgcccgatgc gagcctgttg aaacaacctg aggaattggc
     3961 gctggccaag acagtcgatg aagtcgagcg cgcggcggca gccgcagtcg gaaaggaaga
     4021 tttcgaggct gccatggccg cgatggcgcg cttgcgcgct ccggtcgacg ccttcttcga
     4081 ccacgtcacc gtcaacaccg atgatccggc tctccgcgag aaccgcctgc gcctgctcaa
     4141 ccgcatccgc tcaacgaccc tcaccgtcgc cgatttctcc aagatcgaag ggtgatcgcc
     4201 atgccagctc acgtcagttt gatcaccctt ggcgtcgccg acgttgccaa ggccacgtcg
     4261 ttctacgaaa ggctcggctt cctccggtcg aagaagccga gcgaggcaag cgtcagcttc
     4321 tttcaggcgg gacctgtcgt gctcgcgttg tggggacgcg atgcgcaaag ggacgacgcg
     4381 caagccgcgg agctctggac cggcaatggc ggcatcgtcg tcgcccagaa cctagcgagc
     4441 gaggatgcgg tcgataccat gatggcgaag gctgaggccg cgggggcgcg catcctgaag
     4501 cctgccgcca agaccttctg gggcggctat gacggctatt tcgccgaccc ggacgggcat
     4561 gtgtgggagg tggccttcaa tcccttctgg gaactcgatg aggacggccg ggttagactg
     4621 ccggaatagc tcaatgaccg actacctctt cgtctacggt accttgcgat ccgaatttcc
     4681 tcatcccctc gcgagaaggc ttcaggccca ggccaaattc atcggcaagg gctcgacgcc
     4741 cggtgcgctg tacgatttcg gctggtatcc gggagccagg ttcgatcctc atgcaaggac
     4801 gcacgtcatc ggcgaggtgt tctcgatcaa gaacgccgag cggctgcttg ccgagctcga
     4861 ccactatgaa ggcactgccg agcccggcaa cgccttccgc cgcgtgccgg tcacggtgcg
     4921 gctggatcat ggcggcacgg tcgatgcctg gtcctatgag atgggagact cgaaggcgcg
     4981 gcgccggccg atcgagagcg gcgatttcat tcaccatcgc cggctcaagg accagcggca
     5041 ggtgcggggc taatctcccc tcccctccat ttgtgtcggg cggagcgggc cgctaaaagg
     5101 ggcgatggaa cgcgtgatga aaccggccgc taaggccaag gacccctcgc cccattgggt
     5161 gttcaccttc ggtgccggcc gcgccgaggg cgatgccgct cagcgcgacc tgctcggcgg
     5221 caagggcgcc taccttgccg aaatgtcgcg gctcggtctt ccggtgcctc cagggctcac
     5281 catctcgtcc gaggtctgca ccgcctatta ccggtcgggc aagatgctgc ccgcggctct
     5341 gatgccgatg gtgagcgagg cactcgacgt ggtgggcaag gtcgccggcg cccgcttcgg
     5401 caacgtcgac aatcctttgc tcgtatcggt gcgctcgggc tcgcgcgcct cgatgcctgg
     5461 catgatggac acggtgctca atctcggcct caacgacgag accgtcgaag ggctggcgcg
     5521 gcgctcctcc gaccgcagat tcgcctacga cacctatcgc cgcttcatcc agatgtatgg
     5581 cgatgtggtg ctcggcatcg atcattacct gttcgaggag gtgctcgaga actacaaaag
     5641 tctcaaaggc ttcgagctcg actcggagct tggtcaagag cattgggcgg agctcgtggc
     5701 ccgcttcaag gccctggtcg agcaggagct tggccagcct ttcccgcagg atctgcacgt
     5761 tcagctgtgg ggcgcgatcg ccgcggtgtt cggctcttgg cacaacgctc gcgccatcgc
     5821 ctaccggcgg ctgcacgaca tcccagacga ttgggggacc gcggtcaccg tgcaagccat
     5881 ggtgttcggc aatatgggcg agcggagcgc gaccggcgtc gtgttcaccc gcaatccttc
     5941 caccggggaa aatgcgctgt acggcgagtt cctggtgaat gcccaaggcg aggacgtggt
     6001 cgctgggctc cgtacgccgc aacccttgac cgaggcggcg cggaatgcta ccggcgaccg
     6061 ccgtccgtcg ctcgaggcgc tgatgcctga gaccttcgcc gatctcaaga cggcttgcgc
     6121 caagctcgaa gcgcatttcc gcgacattca ggacatcgag ttcacggtgg aggaaggcag
     6181 gctgtggatg ctgcaaagcc gctcgggcaa gcgcagcatg caggcggcct tgaagatcgc
     6241 cgtcgacatg gttggcgaag gactgatcac cgagaaggaa gccgtgctcg gcatcgatgc
     6301 cgcccagatc gaccagctcc tacacccgac gctcgatccg gactcgccga agctcgtcat
     6361 cggtaagggc ctcccagctt cccccggtgc cgcctccggc aagctcgtgt tcgatgccga
     6421 cgaggcggtg catctcaagg cgcaaggcca caccctcatc ctcgccaggg tcgagacttc
     6481 gccagaggac gtgcagggca tgcacgccgc cgccggcatc atcaccacgc gcggcggcat
     6541 gacgagccac gcggcggtgg tggcgcgtgg catggggcgg ccttgcgtcg tcggcgcggc
     6601 ctcggtgcag atcgatcttg agcgcgagac gttgagcgcc ggcggcgaga ccttgggcaa
     6661 gggcgacgtc gtgaccatcg acggctcgac cggccagatc atcaaggggc gcgtgccgac
     6721 gcgcgagccg acgctttcct cggacttctt gacgttgatg ggctgggccg atcagttccg
     6781 ccgcatgaag gtgcgcgcca acgccgatac gcccaccgac gcccgccagt cgcttgcctt
     6841 cggcgccgac ggcatcgggc tttgccgcac tgagcatatg ttcttcgaag agggccgcat
     6901 cgtgccgatg cgcgagatga tcctcgccga cgataccgag ggcaggcgcg ccgcgctcgc
     6961 caagctcctg cctatgcagc gcaaggactt catcgagctg ttcgagatca tggcaggcgc
     7021 cccggtgacc atccgcctgc tcgatccgcc tttgcacgaa tttcttccgc atggcgaggc
     7081 ggccatcggc gaggtggcgg ctgccatggg ggtcagcgcc gcgcggctga agcgcagggt
     7141 cgctggcttg agcgagttca atcccatgct cggccatcgc ggctcccgcc tcctggtaag
     7201 cttcccggaa atcgccgaga tgcaggcgcg agcgatcttc gaggcagcca tcgaagccgg
     7261 ccgcaagcta gggacgaccg tcgtgccgga aatcatggtg ccgctggtca ccgccaaggc
     7321 agagctcgac ctcgtcaagg agcgcatcga cggcgtggcg cgcgcggtgg aaaaggagca
     7381 aggggtgaag ctcacctact cggtcggcac catggtcgag ctgccccgcg cctgcatcaa
     7441 tgccggcgag atcggcaaga gcgccgattt cttctccttc ggcaccaacg acctgaccca
     7501 gaccacgttc gggctctccc gcgacgacgc cgggcgcttt ctcagcgaat acaccgagaa
     7561 agggatcatc cagcacgacc cctttgtcac gctcgatccc gccgtcggcg agcttatgtg
     7621 gattgccgtt gagcgcgggc ggcggtcgca ccccgacctc aagatcggca tctgcggcga
     7681 gcatggcggc gatcccgaga ccatcaggtt ctgcgaggac atctatctcg actatgtctc
     7741 cagctcgccc taccgggtgc cggtggcgcg acttgctgcc gcacaggccg ccttgagcct
     7801 cacggtaaag ccccgttaac catgtcggca aaagccgtta gggctttcca ttcaccataa
     7861 tttgtcctaa atgatagagc aggataactg tgaaatagat gggactccgg gcaaaccggt
     7921 gggggacaat aagacttaag tcactgattt tcccaccttt ttggctggtt tcatattggg
     7981 gacaaatcct tacgtgcgcc cgataagtgg cgccgtcggg gtacgcgaaa agtcgcttac
     8041 gggcataaag cgggctttcg cggacgtaac ggggatactc cccgggaggg accattgggc
     8101 cggcgctcac ctcgtcggat gtggcacgta gcagctgcgc tcgcgccgct cccgcttatc
     8161 gccttgggct atgcttcgat tccgggcaac aagcttgctt tccggatgga gctgatgctt
     8221 tccggcaatg ggtcaagcca cgcgctcacc cgcgacgccg acgcctccga gcatgttc
//
LOCUS       DATNAE010000050         3526 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_1857886, whole genome shotgun sequence.
ACCESSION   DATNAE010000050 DATNAE010000000
VERSION     DATNAE010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3526
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1857886"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1539
                     /locus_tag="VN518_00955"
     CDS             <1..1539
                     /locus_tag="VN518_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HXE03172.1"
                     /translation="HCVCLLGNSFGYFDRQEDDVRVLTAVKRALASGGTLVMDLTDGE
                     WMRGHFDPRSWEWIDQNHFVCRERALAGDGERLISREVVVHAERGVIADQFYAERLYS
                     RERIQALLESVGFTNLRFHAFVVPESPRNQDLGMMAHRMFLTCEAPRKVERLPRQVLF
                     PEVTVLMGDPRLPDTVKRNGRFNDEDFETIARFKSALAEIPGYRFNFIDNHASLIADL
                     RTSRPQFVINLCDEGFNNDAFMELHVPALLEMFDIPYSGAGPTCLGLCYNKALVRAIA
                     ASLDVPVPLETYFSMDDQAATIPSVFPALIKPNYGDSSIGITQEAVVHTWEQALNYLG
                     RLRAQMPGVPILIQEFLTGAEYSVGILGNPGLASRALPVLEVDYSKLDRGLPQLLAYE
                     SKWIPDSPYWSEISYREAQIDEDTRRRLVDHSNILFERLGCRDYARFDFRADAEGEIK
                     LLEVNPNPGWCWDGKLNMMATLAGLRYSDMLRLIIEAAQERVAQQRPSLAQPALERVA
                     AGQR"
     gene            2141..3292
                     /locus_tag="VN518_00960"
     CDS             2141..3292
                     /locus_tag="VN518_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008193218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HXE03173.1"
                     /translation="MKKKKNDRNKKIERFLAEKQPETPCLVVDLDVVAEAYRMLREYL
                     PLAKVFYAVKANPAEPIVDRLHGLGSSFDVASRGEVDLCLGLGVSAERISFGNTIKKE
                     RDIAYAYERGVRLFAFDSSAELEKLARSAPGARVFCRILVESEGAEWPLSRKFGCAPE
                     MAIELLRSAKAQGLDPYGVSFHVGSQQTNLDQWDGAIGRAASMFSALAATDINLRMVN
                     IGGGFPARYRNDVPGVERYAAAVMAAVTRHFGNDLPEIIIEPGRSLVGDAGVIQSEVV
                     LISQKAFGDAKRWVYLDVGKFNGLAETMDESIKYRIRTPADGGASGPTVLAGPTCDSA
                     DILYEKTEYRLPMALKVGDKVQILSTGAYTSSYASVGFNGFTPIKTYCI"
     gene            complement(3280..>3526)
                     /locus_tag="VN518_00965"
     CDS             complement(3280..>3526)
                     /locus_tag="VN518_00965"
                     /inference="COORDINATES: protein motif:HMM:NF018420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1194 domain-containing protein"
                     /protein_id="HXE03174.1"
                     /translation="PPQQVRDEMVAAGITVNGLAILKTEPWLDSYYDDNVVGGPAGFL
                     MQVDDFPSFAAAMQQKLLAEVAATRPQSRAAALRQMQ"
BASE COUNT          613 a         1199 c         1099 g          615 t
ORIGIN      
        1 cattgcgtct gcctcctcgg caattccttc ggctatttcg accgccagga ggacgatgtc
       61 cgggtcctga cggcggtcaa gcgcgcgctc gcctcgggcg gcaccctggt catggacctg
      121 accgacggcg agtggatgcg cggccatttc gatccgcgct cctgggagtg gatcgaccag
      181 aaccacttcg tctgccgcga gcgcgcgctc gccggcgatg gcgagcggct catctcgcgc
      241 gaagtcgtcg tccatgccga gcgcggcgtc atcgccgacc agttctatgc cgagcggctt
      301 tattcccggg agcgcatcca ggcgctcctc gaaagcgtcg gcttcaccaa tctccgcttc
      361 catgccttcg tcgtcccgga atcgccgcgc aaccaggacc tcggcatgat ggcgcaccgc
      421 atgttcctga cctgcgaggc gccgcgcaag gtcgagcggc tgccgcgcca ggtgctcttc
      481 cccgaagtca cggtgctgat gggcgatccg cggctccctg acacggtcaa gcgcaacggc
      541 cgtttcaacg acgaggattt cgagaccatc gcgcgcttca agagcgcgct cgccgagatc
      601 cccggctatc gcttcaactt catcgacaac cacgcctcgc tgatcgccga tctgcgcacc
      661 agccgcccgc aattcgtcat caacctctgc gacgagggct tcaacaacga cgccttcatg
      721 gagctgcacg tcccggcgct tctggagatg ttcgacatcc cctatagcgg cgccgggccg
      781 acctgcctcg ggctttgcta caacaaggcg ctggtgcgcg cgatcgcagc atctctcgac
      841 gtgccggtgc cgctcgagac ctatttcagc atggacgacc aggccgccac catcccctcg
      901 gtctttccgg cgctgatcaa gccgaactat ggcgacagct cgatcggcat cacccaggag
      961 gccgtcgtcc atacctggga gcaggcgctc aactatctcg gccgcttgcg cgcgcagatg
     1021 ccgggcgtgc cgatcctcat ccaggaattc ttgaccggcg ccgaatactc cgtcggcatt
     1081 ctcggcaatc ccggcttggc atcgcgcgcg ctccccgtgc tcgaagtcga ctactccaag
     1141 ctcgatcgcg gcctgccgca gctcctggcc tacgagtcca aatggatccc cgattcgccc
     1201 tactggtcgg agatcagcta tcgcgaggcg cagatcgacg aggacaccag gcgccggctg
     1261 gtcgatcact ccaacatcct cttcgagcgc ctgggctgcc gcgactacgc ccgcttcgac
     1321 ttccgcgccg atgccgaggg cgagatcaag ctgctcgagg tcaatcccaa tcccggctgg
     1381 tgctgggacg gcaagctcaa catgatggcg acgctggcgg gccttcgtta ttccgacatg
     1441 ctccgcctca tcatcgaagc cgcacaggag cgcgtggcgc agcagcgccc gagtctcgcg
     1501 cagccggcgc tcgagcgcgt cgccgccggc cagcgctgac ctctggacga gcgatcaggg
     1561 catggcggcg gcgtccgcga ggctgccgcg agcgcgctcg cgcaacgatg acggggcgct
     1621 cggcgatcca agtgcaaaaa attttatgcg aaaattcgct cggggctaaa ttttcccttg
     1681 cgcgccgagc atttgagcgt atttatcgaa ttcgcgacgc actcgcacgt gcctatggcc
     1741 cttgtggtta tgcaacgacg taacgcgtcc tttttggcag atccggtcta gtggaccggt
     1801 cccgagaggg aggacgatat ggttgcaatc cgatgcaggg ccgcagcgcc ctggccggac
     1861 gatacacctc ggcagatcag ggtcgcccgc ggcagcggcg gccgagcttc caacgagcct
     1921 agcagtcaga tctaagcgac gggtcccaag cgcggcggat cgcccccgtg ttcggacatc
     1981 gacttagcgc ctttgctctc cccgtgggag ggcagcggca tagcgcgtcg acagcctcgc
     2041 tcgacggtgc ccgcgactgc tcgaagagaa cgtgccgagc ccgggtttcc ccagagagca
     2101 tgcctgtcgc agatcgttgg ctcaggaaag attgcatgcg atgaaaaaga agaagaacga
     2161 ccgaaacaag aagatcgagc gcttcctcgc ggaaaagcag ccggagacgc cgtgcctcgt
     2221 cgtcgatctc gacgtcgtcg ccgaagccta tcggatgctg cgcgaatact tgccgctcgc
     2281 caaggtcttc tatgcggtga aagccaatcc ggcggagcca atcgtcgacc ggctgcacgg
     2341 gctcggctcg agcttcgatg tcgcgagccg cggcgaggtc gatctctgcc tcggcctcgg
     2401 cgtatccgcc gagcgcatct ccttcggcaa caccatcaag aaggagcgcg acatcgccta
     2461 cgcctatgag cggggcgtgc ggctcttcgc cttcgacagc agcgccgaat tggaaaagct
     2521 cgcccgctcg gcgccgggcg cgcgcgtctt ctgccgcatc ctcgtcgaga gcgagggcgc
     2581 ggaatggccg ctgtcgcgca aattcggctg cgcgccggag atggcgatcg agctcctgcg
     2641 cagcgccaag gcacagggcc tcgaccccta tggcgtctcc ttccatgtcg gatcgcagca
     2701 gaccaatctc gaccagtggg acggcgccat cggccgcgcc gccagcatgt tctcggcgct
     2761 ggccgcgacc gacatcaatt tgcgcatggt caatatcggc ggcggctttc ccgcgcgtta
     2821 ccgcaacgac gtgcccggcg tcgagcgcta tgccgcggca gtgatggcgg ccgtcacccg
     2881 gcatttcggc aacgatctgc cggagatcat catcgagcct ggccgctcgc tggtgggcga
     2941 tgccggcgtc atccagagcg aggtggtgct gatctcgcag aaagccttcg gcgacgccaa
     3001 gcgctgggtc tatctcgatg tcggcaagtt caacggcctc gccgagacca tggatgagag
     3061 catcaagtac cgcatccgga cgccggccga tggcggcgcg agcggcccga ccgtgctcgc
     3121 cgggccgacc tgcgatagcg ccgacatcct ctacgagaag accgagtacc gtttgccgat
     3181 ggcgctcaag gttggcgaca aagtgcagat cctctcgacc ggcgcctata cctcgagcta
     3241 cgcctccgta ggcttcaacg gcttcacgcc gatcaagacc tactgcatct gacggagcgc
     3301 ggcggcgcgg ctttggggcc gcgtcgccgc cacctctgcc aggagcttct gctgcatcgc
     3361 cgcggcgaag gacgggaaat cgtccacctg catgagaaag ccggcgggcc cgcccacaac
     3421 gttgtcatcg taatagctgt cgagccacgg ctcggtcttg aggatggcga gaccgttgac
     3481 ggtgatgccg gcggcgacca tctcgtcgcg cacctgttgc ggcggt
//
LOCUS       DATNAE010000051         5059 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_1858555, whole genome shotgun sequence.
ACCESSION   DATNAE010000051 DATNAE010000000
VERSION     DATNAE010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5059
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1858555"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..194)
                     /locus_tag="VN518_00970"
     tRNA            complement(118..194)
                     /locus_tag="VN518_00970"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(158..160),aa:Arg,seq:ccg)
     gene            396..2054
                     /locus_tag="VN518_00975"
     CDS             396..2054
                     /locus_tag="VN518_00975"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXE03175.1"
                     /translation="MEKKKVLILGGGFGGVFAAKELARRGADRFDVELINNNNYFVFQ
                     PLLPEVAAATIHSSDAVAPLRLLLRGTQIRQADVMGIDFAKKTVTVVQGFRRVPVDLP
                     YDELIIALGMSVDLNRFPGLPEHALTMKNLSDAHRLRTHVISCLEMADVATEPELKRE
                     LLTFVVVGAGFTGVETVAEVNDLIHRALKYYPNIKHEEIKVYLIEYANRILPTFPADL
                     AEYATLRLRLHGIDVMTGTGTKSATSTGVELADGRLIPTRTIVATIGNGPHRLVQSLD
                     LDMQWGRIKTDRFMRVSGHDGIWAVGDAALIQLVEQPAEDPLDYAPQTAQFAVREGRQ
                     LADNLIAKDKAAELTPFAYSSKGSLASLGMSKAVAEVYGIKLSGFLAWLLWRGFYLSF
                     LPGFATKVRVLVNWMLNAFTPPNIVLVQSSMPGTRYIHYRKGDKVLEPGMLIDGFYTV
                     VKGSLRLTIDNLETGEHFEKVFGPGEHFGERVLLKSSLRTGLVVALEDSTVLWISQKD
                     FMRFARAFPFMETYFKDYIEKTFGPDMVFAPGSAERSHELEHTH"
     gene            2262..>5059
                     /gene="polA"
                     /locus_tag="VN518_00980"
     CDS             2262..>5059
                     /gene="polA"
                     /locus_tag="VN518_00980"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009798272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HXE03176.1"
                     /translation="MTITEGKQTAAGRPVAKGDHVYLIDGAGYIFRAYHALPPLTRPS
                     DGLPVGAVHGFCGMLWKLLREAGELSPPTHFAVIFDLSEHTFRNELYADYKAHRPDMP
                     EDLLPQFPLIRNAVKAFNVACLEQKGYEADDLIATYAREAQEAGADVTIVSSDKDLMQ
                     LVRPGVRMYDTMKNKVIGEDEVMERFGVPPSKVIEVQALIGDPTDNVPGVPGIGVKTA
                     ALLINEYGDLDTLLARASEITQPKRRESLITFAGQARLSRTLVILDTNVPLDVPLAET
                     LVRQPEAETLLSFLRKLEFSTLLRRIADGLGVEAPAGAAPAPATQRRKKDDYDHPLRR
                     APSGGFVTERTEPVHPAKEGSPLHLAEERAAKLAALPFDRSKYETVVHPERFAALLDE
                     ARDQGHVAFRAKVTSADPMQGELVGVALALRPGQAAYIPLAHRAADELDLGGEEGPRI
                     QTREALDLLKPLLEDPAVLKIVQNAKFDMVVLARYGIALTSFDDPCLMSYALDAGRAE
                     HLPEQLAGTLLSYTCLTHKEIVGTGRAAVTFDRVPVARATEFAGEEADIGLRLWLVLK
                     PRLIAEHVDTVYETLERPIAPVLALMERTGIRVERTTFSSLSGSFAQSLARLEDEIRE
                     LAGEDFNIGSPKQLGEILFDKMSLPGGRRTKTGAWSTDASALEELAAEGHDLPRKVLD
                     WRLLAKLKSTYTDALPTYIHPETGRVHTSYALAATTTGRLSSLEPNLQNIPVRTSEGR
                     AIRKAFVSDKGKRLISADYSQIELRVLAHMADTPTLRQAFADGLDIHAMTASEMFGVP
                     IEGMDPAIRRRAKAINFGIVYGISSVGLAAQLGIGRSEAGAYIKTYFERFPGIRDYME
                     ATKAEARRQGYVKTLFGRKVHYPEINTKNPSLRGNFERAAINAPIQGSAADIIRRAMI
                     RMVPALAEAGLNARMLLQV"
BASE COUNT          937 a         1735 c         1549 g          838 t
ORIGIN      
        1 cgagttgggc gagaatggaa gcatcttccc aaggccgaag gggaaggcgc gttagaacgc
       61 ctgccgaaca aattccgcgc caattccgcg ccatgaatga ttctgatggg gagaaactgg
      121 cgcacccgac aggattcgaa cctgtgacct ctgccttcgg agggcaacgc tctatccaac
      181 tgagctacgg gtgctcgtcg cgcgaaccta aaccaaaggc ctccgcctaa gcaacggcgg
      241 cgcgacagtc aggcaccgga agccacaaat caagggcgcc tgggcagaag gggcttccgc
      301 ttccccttct gacttcttac gtctgattat ggcttccagc ttccgaacgg cgtaggtatg
      361 cctgcaaatg ccattaacgg caggggtctt tgcccatgga aaagaagaag gttctcattc
      421 tcggcggcgg tttcggcggc gtttttgccg ccaaggagct ggcgcgccgc ggtgccgacc
      481 ggttcgacgt cgagctgatc aacaacaaca actatttcgt gtttcagccg ctgctgcccg
      541 aggtcgctgc cgccaccatc cactcctccg acgcggtggc gccgttgcgc ctgctcttgc
      601 gcggcaccca gatccgccag gccgacgtga tgggcatcga tttcgccaag aagacggtga
      661 ccgtggtgca gggcttccgc cgggttccgg tcgacctccc ctatgacgag ctgatcatcg
      721 cgctcggcat gagcgtcgat ctcaaccgct tccccggcct gcccgagcac gcgctcacca
      781 tgaagaacct ctccgacgcg catcggctcc gcacgcatgt gatcagctgc ctggaaatgg
      841 ccgatgtggc gaccgagccg gagctgaagc gcgagctgct caccttcgtg gtggtcggcg
      901 ccggcttcac cggggtggag accgtggccg aggtcaacga tctgatccac cgcgcgctga
      961 aatactatcc aaacatcaag catgaggaga tcaaggtcta cctcatcgaa tatgcgaacc
     1021 gcatcctgcc gaccttccct gccgacctcg ccgaatatgc gacgctccgg ctgcggctgc
     1081 acggcatcga cgtgatgacc ggcaccggca ccaaatcggc aacttcgacc ggcgtggaat
     1141 tggccgacgg caggctgatc ccgacccgga ccatcgtcgc cacgatcggc aacggcccgc
     1201 accggctggt gcaatcgctc gatctcgaca tgcagtgggg gcgcatcaag accgaccgct
     1261 tcatgcgcgt ttccggccat gacggcatct gggcggttgg cgacgcggcg ctgatccagc
     1321 tggtcgagca gccggccgag gacccgttgg actacgcccc ccagaccgcg caatttgccg
     1381 tgcgcgaagg gcgccagctc gccgacaatc tgatcgccaa ggataaagcc gcggagctca
     1441 cgcccttcgc ttattcgtcg aaaggctcgc tcgcctcgct cggcatgagc aaggccgtcg
     1501 ccgaggtcta cggcatcaag ctctcaggct tcctcgcctg gctgttgtgg cgcggcttct
     1561 atctctcctt cctgccggga tttgccacca aggtgcgcgt gctggtgaac tggatgctga
     1621 acgcattcac gccgccgaat atcgtgctgg tgcaatcctc gatgcctggc acccgctaca
     1681 tccactaccg caagggcgac aaggtgctcg agcctggcat gctgatcgac ggcttctata
     1741 ccgtggtcaa aggatcgctc aggctcacca tcgataacct cgagaccggc gagcacttcg
     1801 agaaagtgtt cgggcccgga gagcatttcg gcgagcgggt gctgctcaaa tcctcgctcc
     1861 gcaccgggct tgtggtggcg ctcgaagatt ccaccgtgtt gtggatttcg cagaaggact
     1921 tcatgcggtt cgcccgcgcc ttcccgttca tggagaccta tttcaaggac tatatcgaga
     1981 agacctttgg gccagacatg gtctttgccc cgggcagcgc cgagcgcagc cacgagctcg
     2041 agcacacgca ctgacgcagc cagcacccca cccggctcac ccggctaagg ccgggcgagc
     2101 caccctcccc tggaggggag ggataagacc gcccgagcca actcccataa tccctccccg
     2161 cgaggggagg gtggacgcgg cgcgaagcgg cgcggacggg tggggagaag gggcgcggcg
     2221 atgctgacgg aggccccgcc aatccctata ttcccctcat catgacaatc accgaaggca
     2281 agcaaacggc cgccggcagg ccggtggcaa agggcgacca cgtctatctg atcgacggcg
     2341 cgggctacat cttccgcgcc taccatgcgc tcccgccgct cacccgcccc tcggacgggc
     2401 tgccggtggg cgccgtgcac ggcttctgcg gcatgctgtg gaagctgttg cgcgaggcgg
     2461 gcgagctttc cccgccaacg catttcgcgg tgattttcga cctttccgag cacacgttcc
     2521 gcaacgagct ctatgctgat tacaaggcgc atcgccccga catgccggag gacctcctcc
     2581 cgcaatttcc gctgatccgg aacgcggtga aggccttcaa cgtcgcctgc ctcgagcaga
     2641 aaggctacga ggccgacgac ctcatcgcca cttacgcgcg cgaggcgcag gaagccggcg
     2701 ccgacgtcac catcgtctcc tccgacaagg acctgatgca gctcgtgcgc ccgggcgtgc
     2761 gcatgtacga caccatgaag aacaaggtga tcggcgagga cgaggtgatg gagcgctttg
     2821 gcgtgccgcc ctcgaaggtc atcgaggttc aggcgctgat cggcgacccc accgacaacg
     2881 tgcccggcgt gccaggcatc ggggtgaaaa ccgcggcgct gctcatcaac gaatatggcg
     2941 atctcgacac gctgctcgcc cgcgcgtcgg agatcaccca gccgaagcgg cgagagagtc
     3001 tcattacttt cgccggccag gcgcggctgt cgcggacgct ggtaatcctc gatacaaacg
     3061 tgccgcttga cgtgccgctc gccgagactc tggtgcgcca gccagaggca gagacgctgc
     3121 tgagcttcct gcgcaagctc gaattcagca cgttgctccg ccgcatcgcc gatggtctcg
     3181 gcgtcgaggc tcccgccggt gccgcccctg cgccggcgac acagcgacgc aagaaagacg
     3241 actacgacca cccgctcagg cgcgcaccca gcggaggatt cgtcaccgag cggaccgagc
     3301 cggtccatcc ggcgaaggaa ggctcgccgc tgcacctcgc cgaagagcgc gcggccaagc
     3361 ttgccgctct ccccttcgac cgcagcaaat acgagacggt ggtccatcct gagcgctttg
     3421 ccgcgctgct cgacgaagcg cgcgatcagg gccatgtcgc ctttcgcgcc aaggtcacat
     3481 ccgccgatcc gatgcaaggg gagcttgtcg gtgtggcact cgcccttcgc cccggacagg
     3541 cggcctacat tccgctcgcc caccgtgcag ccgatgagct cgatctcggc ggcgaagagg
     3601 gcccgcggat ccagacgcgc gaggcgctcg atctgttgaa gccgctgctg gaagaccctg
     3661 ccgtgctcaa gatcgtgcag aatgccaaat tcgacatggt ggtgctcgcc cgctacggca
     3721 tcgcgctgac cagcttcgac gacccttgcc tgatgtctta cgcgctcgac gccggccgcg
     3781 ccgagcatct gcccgagcag cttgccggca cgctcctctc ctatacctgc ctcacccaca
     3841 aggagatcgt gggcaccggc cgcgccgccg tcactttcga ccgcgtgcct gtcgcgcgcg
     3901 ccaccgagtt tgccggcgag gaagccgaca tcggcctgcg gctctggctc gtgctcaagc
     3961 cgcggctgat cgccgagcac gtcgacaccg tctacgagac cttggagcgg ccgatcgcgc
     4021 cggtgctggc gctcatggag cggaccggca tcagggtgga gcgcacgacg ttctccagcc
     4081 tctccggcag cttcgcccag agcctggcgc gtctcgagga cgagatcagg gagcttgccg
     4141 gtgaggattt caacatcgga tcgccgaagc agctcggcga gatcctgttc gacaagatga
     4201 gcttacccgg cggacggcgc accaagaccg gcgcctggag caccgacgca agcgcgctcg
     4261 aggagcttgc cgccgagggc cacgatctgc cgcgcaaggt tctcgactgg cgcctgctcg
     4321 ccaagctgaa aagcacctat accgacgcgc tccccaccta catccatccc gagacgggcc
     4381 gcgtgcacac ttcctacgcg ctcgccgcca caaccaccgg ccggctgtca tcgctcgagc
     4441 cgaatttgca gaacatccca gtgcggacga gcgagggccg cgccattcgc aaggccttcg
     4501 tgtccgacaa gggcaagagg ctgatctcgg ccgactacag ccagatcgag cttcgcgtgc
     4561 tcgcccacat ggcggatacg ccgacgctgc gccaggcctt cgccgacggg ctcgacattc
     4621 acgccatgac ggcttcagag atgttcggcg tcccgatcga aggcatggac ccggccatca
     4681 ggcgccgcgc caaggccatc aatttcggca tcgtgtacgg catctcctcg gtcggactcg
     4741 ccgcccagct cggcatcggc agaagcgagg ccggcgccta catcaaaacc tatttcgagc
     4801 gcttccccgg catccgcgac tacatggagg cgaccaaggc ggaggcgcgc cgccaaggct
     4861 atgtgaagac gctgttcggg cggaaggtgc attaccccga gatcaacacc aagaatccgt
     4921 cactccgcgg caatttcgag cgcgctgcca tcaacgcgcc gattcaaggg agcgccgccg
     4981 acatcatccg ccgcgccatg atccgcatgg tgccggcgct cgccgaagcc ggcctcaacg
     5041 cgcgcatgct gctgcaggt
//
LOCUS       DATNAE010000052         3035 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3063443, whole genome shotgun sequence.
ACCESSION   DATNAE010000052 DATNAE010000000
VERSION     DATNAE010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3063443"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..1406
                     /gene="murD"
                     /locus_tag="VN518_00985"
     CDS             3..1406
                     /gene="murD"
                     /locus_tag="VN518_00985"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HXE03177.1"
                     /translation="MFAGRDVAVFGLGLSGIAAAHALAAGGARVLAWDDTQEARDKAA
                     AQGIALHDLAGADWSRIAALVLAPGIPLTHPKPHWSVRKAQEAGVEVIGDTELFFRER
                     TRQGASAKVVVITGTNGKSTTTALTAHLLQLGGRPVALGGNIGRAVLDLPPFATGLTY
                     VLELSTFQIDLTPSLAPDAAALINITPDHLDRHGTIENYAAIKARVFDHLLVNGTAVI
                     GVDDDYSRAIADGLRGSYAVKRIAVGHPVQTGVWAKDGMLPEIENGVERGRVGLDGIG
                     PLRGAHNWQNAGVAYALARSQGLDASAIAKGLGTFAGLAHRMEQVARAGSVLFVNDSK
                     ATNADAAAKALASFTDIYWIVGGRPKAGGLDGLEPFFPKIARAYLIGEAADEFAAQLG
                     GQVDHVRCVTLDRAIEAAAADARRSRGAEPVVLLSPACASYDQFANFEARGRSFRDLV
                     MRLPGVSPLQPREGRAA"
     gene            1403..2596
                     /gene="ftsW"
                     /locus_tag="VN518_00990"
     CDS             1403..2596
                     /gene="ftsW"
                     /locus_tag="VN518_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HXE03178.1"
                     /translation="MRITRAEKSVLSDWWFTVDRLMFFGLLLLMGAGLVLSLAASPPI
                     AAKYNLEPFYFVRRQAALLLPAVVIMFAASTLTPKQIRRASLIIFVVGIALMLATMFI
                     GPEIKGARRWLQIGFVSIQPSEFVKPAFVVLTAWLFNESQKRSDVPGIELALALYGIF
                     ALLLVLQPDFGQTLLVSLVWLALFFMAGINLFWIGALAFAGVAGIASAYFLVPHVASR
                     IDRFLNPASGDTYQADKALDSFLHGGWFGRGPGEGTVKDVLPDSHTDYIFAVAAEEYG
                     LIACLILLAVFAFIVLRGLSKASQEPDGFIRHATAGLMMLFGLQTLINMAVNVGLLPA
                     KGMTLPFISYGGSSLLAMALTMGFVLGLTRRRPIAGRLPRSAPSEAEGEPGSHTGKAA
                     SEQVA"
     gene            2593..>3035
                     /locus_tag="VN518_00995"
     CDS             2593..>3035
                     /locus_tag="VN518_00995"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXE03179.1"
                     /translation="MSERPILLAAGGTGGHLFPAASLAQELRRRGFAVELATDMRAEK
                     YGGDFPARAIHRIPSATLTSRSPIAVTATFTRLGIGYFGALRMLLRLKPRAVIGFGGY
                     PTLPPIIAASTLRIPTAIHEQNAVMGRANRQLSSRVTVIATSFEGV"
BASE COUNT          462 a         1052 c          949 g          572 t
ORIGIN      
        1 ccatctttgc cggccgcgat gtcgcggtgt ttggactggg cttgagcggc atcgccgctg
       61 cccacgccct tgccgccggc ggcgcgcggg tgctcgcctg ggatgacacg caagaggcgc
      121 gcgacaaggc tgcggctcaa ggcattgccc tgcacgacct tgccggcgcc gattggagca
      181 ggatcgcggc gctcgtgctc gctcccggca ttcctctgac ccatcccaag ccccattgga
      241 gcgtgaggaa ggcgcaagaa gcgggcgtcg aagtcatcgg cgacacggag ctcttcttcc
      301 gcgaaaggac gaggcagggg gcgagcgcca aggtcgtggt tatcaccggc accaacggca
      361 aatcgaccac caccgcactc accgcccatc tgttgcagct gggaggcagg ccagtcgcgc
      421 ttggcggcaa tatcggccgc gccgtcctcg accttccgcc ttttgctacg ggcctgacct
      481 atgttctcga gctgtcgacg tttcagatcg acttgacgcc gtcgctcgcc ccagacgccg
      541 cggcgctcat caacatcacc cccgatcatc tcgaccgcca cggcaccatc gagaactacg
      601 ccgccatcaa ggcgcgcgta ttcgatcacc tgctggttaa cggcaccgcc gtgatcggcg
      661 tcgacgacga ttattcccgc gccatcgcag atgggcttcg cgggtcatac gccgtgaagc
      721 gcatcgccgt tggccatccc gtccagacag gcgtgtgggc gaaggacggg atgttgcccg
      781 agatcgagaa cggcgtcgag cgcggccgcg tcggccttga cggcatcggc ccgcttcgcg
      841 gcgcgcacaa ttggcagaac gcgggcgtgg cctacgcgct tgcccgctcg caagggctcg
      901 acgcctcggc catcgccaag gggcttggaa cctttgccgg ccttgcccac cgcatggagc
      961 aggtggcgag ggcagggtcg gtcctttttg tcaacgactc aaaggccacc aatgccgatg
     1021 ccgccgccaa ggcgttggcg agcttcaccg acatttattg gattgtcggc ggcaggccga
     1081 aagcaggagg ccttgatggc ctggagccgt ttttccccaa gatcgcccgc gcctatctga
     1141 tcggcgaggc agcagatgaa ttcgccgcgc aactcggcgg ccaggtcgat catgtgcgat
     1201 gcgtgacgct cgaccgcgcc atcgaagccg ctgccgccga cgcgcgacgc tcgcgcgggg
     1261 ccgagccagt agtgctgctg tcgccggctt gcgcctccta cgaccagttc gccaatttcg
     1321 aagcgcgagg gcgaagcttc cgcgacctcg tcatgcgcct tccaggcgtg agtccgctgc
     1381 agccaagaga gggcagggcg gcatgaggat cacgcgtgcc gagaagagcg tgctctccga
     1441 ctggtggttc accgtcgacc gcctgatgtt cttcggcctg cttctactaa tgggcgccgg
     1501 cctcgtgctg tcgctcgccg cgagcccgcc catcgccgcc aaatacaatc tcgagccgtt
     1561 ctacttcgtg cgccgtcagg cggcgctgct tctgcccgcg gtggtcatca tgtttgcggc
     1621 ctcgaccctc accccgaagc agatccgccg cgcgagcctc atcatctttg tcgtcggcat
     1681 cgcgctgatg ctcgccacca tgttcatcgg ccccgagatc aaaggcgcta ggcgctggct
     1741 gcagatcggc ttcgtgtcga tccagccgtc ggaattcgtc aagccggctt ttgtggtgct
     1801 caccgcctgg ctgttcaacg aaagccagaa gcgcagcgac gtgccgggca tcgagcttgc
     1861 gcttgcgctt tacggcatct ttgcgctgct gctcgtgctg cagcctgatt tcgggcagac
     1921 gctgctcgtc agcctcgtgt ggctggcgtt gttcttcatg gccggcatca atctcttctg
     1981 gatcggtgcg ctcgcctttg ccggcgtcgc cggtatcgcg tcggcctatt ttctcgtgcc
     2041 gcatgtggcc tcgcgcatcg accgcttcct caatccggcc tccggcgaca cctaccaggc
     2101 cgacaaggcg ctcgactcct tcctgcatgg cggctggttc ggccgcggcc ctggtgaagg
     2161 cacggtcaag gacgtgctcc ccgactcgca caccgactac atcttcgccg tagcggccga
     2221 ggaatatggc ctgatcgcct gcctcatctt gctcgcagtg tttgccttca tcgtgctgcg
     2281 cggcctgtcc aaggcgtcgc aggagcctga cggcttcatt cgccacgcga ccgccgggct
     2341 gatgatgctg ttcggcctgc agacgctgat caacatggcg gtgaatgtcg gcttgctccc
     2401 agccaagggc atgacgttgc cctttatttc ctatggcggc tcatcgctgc tcgccatggc
     2461 gctgaccatg ggctttgtcc tcgggctcac gcggcgaagg ccgattgccg gacgcctgcc
     2521 gcggagcgcg ccaagcgaag ccgagggcga acccggctcc catacaggca aggctgcctc
     2581 ggagcaggtg gcttgagcga gcggccgatc ttgctcgccg ctggcggcac gggaggccat
     2641 ctattccccg cggcctcgct cgcccaggag cttcggcgcc gagggttcgc cgtcgagctc
     2701 gccaccgaca tgcgcgccga gaaatatggc ggcgactttc ccgcccgcgc catccatcgc
     2761 attccgtccg ccacgctgac cagccgctct cccatcgccg tcactgccac cttcaccagg
     2821 ctcggcatcg gctatttcgg tgcgctgcgc atgctgttgc gcctgaagcc gagggcggtg
     2881 atcggctttg gcggctatcc caccctgccg ccgatcatcg ccgcgagcac gctccgcatc
     2941 ccgaccgcca tccacgagca gaacgcggtg atgggacgcg ccaaccggca gctttcgtcg
     3001 cgtgtcacgg taatcgcgac ctcgttcgag ggcgt
//
LOCUS       DATNAE010000053         2907 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3971549, whole genome shotgun sequence.
ACCESSION   DATNAE010000053 DATNAE010000000
VERSION     DATNAE010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3971549"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2025
                     /gene="polA"
                     /locus_tag="VN518_01000"
     CDS             <1..2025
                     /gene="polA"
                     /locus_tag="VN518_01000"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HXE03180.1"
                     /translation="EAGPPRKDTRKRESAYDHPLRKMPAGGFVASRTIVDRAEDGGSP
                     AALAANFASQLGSLGFNRSSYEAVTELSRLTDLIDTARYQGHLSFRAKLNSADPMQGE
                     LIGIAFAIVPGHAAYVPLAHRSGEGLDLGGEGEAPQIKMRDALDVLKPLLEDESVLKI
                     VQNAKFDMVALSRYGIALTAIDDPCLMSYALDAGRAEHLPEQLAGRLLSHTCLTEKEV
                     MGTGKNSVGIDRVSVARATEYAGEETDIGLRLWLVLKPRLAAESVTTVYETLERPLAR
                     VLAEMERAGVKVERATLASLSGSFAQTIARLEDEIRALAGEDFNIGSPKQLGEILFDK
                     MSLAGGRRTKTGAWSTDAAALEELAAEGHDLPRKVLDWRLLAKLKSTYTDAIPTYIHP
                     DTGRVHTTYALAASTTGRLSSVEPNLQNIPVRTAEGRAIRKAFVSEKGKKLISADYSQ
                     IELRVLAHMADTPTLRQAFADGLDIHAMTASEMFGVPIKGMDPGIRRKAKAINFGIIY
                     GISSVGLAAQLGISRSEAGAYIKTYFERFPGIRDYMEAMKAEARRTGFVKTLFGRKVH
                     YPEINTKNPSLRGNYERAAINAPIQGSAADIIRRAMIRMGPALAEAGLSARMLMQVHD
                     ELVFEAPEGEVDKTMEVAQSVMESAPEPVLRLSVPLKVDARSGDNWEAAH"
     gene            2116..2349
                     /locus_tag="VN518_01005"
     CDS             2116..2349
                     /locus_tag="VN518_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03181.1"
                     /translation="MARCEQCGNDYDKSFEVKMGGRTMIFDSFECAIHMLAPVCPHCG
                     CRVIGHGVQHEDSIYCCVNCARRDGVTGLRDRD"
     gene            complement(2360..2611)
                     /locus_tag="VN518_01010"
     CDS             complement(2360..2611)
                     /locus_tag="VN518_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03182.1"
                     /translation="MVNIYLFIELLRAASAADTADQIKSLSLANCKFANVVVLSFDKL
                     VAQLDCSTPEDGTKVVLEELPKVEGVVQTNVIAVVKPVH"
     gene            2696..>2907
                     /locus_tag="VN518_01015"
     CDS             2696..>2907
                     /locus_tag="VN518_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03183.1"
                     /translation="MRRLVMLSAVLIGFGVPPSPASALPAPMSQEQLLEKSDLVALVR
                     IKSVTCVSVAKDDRTGEDLPSYSASA"
BASE COUNT          547 a          925 c          952 g          483 t
ORIGIN      
        1 gaagcgggtc cgccacggaa ggacacgcgc aagcgcgaga gcgcctacga tcatccgttg
       61 cgcaagatgc cggcgggggg atttgtcgca tctcgcacca tcgtcgatag agcggaagac
      121 ggaggttcgc cggcggcgct cgcggccaat ttcgcgagcc aactcggcag tctcggattc
      181 aatcggtcga gctacgaggc cgtgaccgag ctttcccgtc tcaccgactt gatcgacacc
      241 gcgcgctacc agggacatct ctcttttcgc gctaaactga actcggcaga cccgatgcag
      301 ggcgaactga tcggaatcgc gtttgccatc gtgccggggc atgcggccta tgtgccactc
      361 gcgcaccgca gcggggaagg actcgatctc ggcggcgagg gcgaggcgcc gcagatcaag
      421 atgcgtgacg cgctcgatgt gctgaagccg ctgctggaag acgaaagcgt gctcaagatc
      481 gtgcagaacg ccaaattcga tatggtggcg ctgtcccgct acggcatcgc actcacggca
      541 atcgacgatc cctgcctgat gtcctatgcg ctcgatgccg gccgcgccga gcatctgccc
      601 gagcaactcg cgggccgcct gctcagccat acctgcctca ccgagaaaga agtgatgggc
      661 acaggtaaga actcggtcgg catcgaccgc gtcagcgtcg ctcgcgccac cgaatatgcc
      721 ggtgaggaga ccgatatcgg ccttaggctc tggctcgtgc tgaagccgcg gctcgccgcc
      781 gaatccgtga ccacggtgta tgagacgctg gagcggccgc tcgcccgcgt tctcgccgag
      841 atggagcgcg ccggcgtcaa ggtcgagcgg gcgacgcttg cctcgctgtc aggctcgttc
      901 gcgcagacca tcgcccggct cgaggacgag atcagggcgc tcgccggaga ggatttcaac
      961 atcggctcgc cgaagcagct cggtgagatc ctgttcgaca agatgagcct cgcaggcggc
     1021 aggcgcacca agaccggcgc gtggagcacg gacgccgccg cgctcgagga gctggcggcc
     1081 gaggggcacg atctgccgcg caaagttctc gactggcggc tgctcgccaa actcaagagc
     1141 acctataccg acgcgatccc gacctatatc cacccggaca cgggtcgagt gcacacgacc
     1201 tatgcgctgg ccgccagcac gacggggcgg ctgtcatcgg tcgagccgaa cctgcagaac
     1261 attccggtgc gcacggcaga gggccgcgcc atccgcaagg cgttcgtgtc ggagaagggc
     1321 aagaagctga tctcggcgga ttacagccag atcgagctca gagtgctggc gcacatggcg
     1381 gacacgccga cgctgcgcca agccttcgcc gacgggctcg acattcacgc catgacggcg
     1441 tcggagatgt tcggcgtgcc gatcaagggc atggaccccg gcatccgccg caaggccaag
     1501 gcgatcaatt tcggcatcat ctacggcatc tcctcggtcg ggcttgccgc ccagctcggc
     1561 atctccagga gcgaggcggg cgcctatatc aagacctatt tcgagcgctt ccccggcatc
     1621 cgcgattaca tggaggcgat gaaggccgag gcgcgccgca ccggcttcgt caagacgctg
     1681 ttcgggcgga aggtccacta ccccgagatc aacaccaaga acccatcgct gcgcggcaat
     1741 tacgagcgcg cggcgatcaa cgcgccgatc cagggttcgg ccgccgacat cattcgccgc
     1801 gccatgatcc gcatgggacc ggcgctcgcc gaagccgggc tgtcggcgcg catgctgatg
     1861 caagtgcatg acgagctggt gttcgaggcg cccgagggcg aggtcgacaa gaccatggag
     1921 gtggcgcagt cggtgatgga gagcgcgccg gagccggtgt tgcgcctcag cgtgccgctc
     1981 aaggtcgacg cccgctcagg cgacaattgg gaagccgcgc attaaaattt ggggatatgc
     2041 cgtacacgga ggttgtggaa cggcgaaagt tctatcagag ttatgcgtgc ttcagcacag
     2101 ccaggggaga gtgccatggc gcgatgcgag caatgcggca acgactacga caagagcttc
     2161 gaggtgaaga tgggcggccg caccatgatc ttcgacagtt tcgaatgcgc gatccatatg
     2221 ctggcgccgg tctgcccgca ttgcggctgc cgcgtcatcg ggcatggcgt ccagcatgag
     2281 gactccatct actgctgcgt gaattgcgcg cggcgtgacg gcgtgacggg cctgcgcgac
     2341 cgggattgac cgctcgccgc taatgcaccg gcttgaccac ggcgatgacg ttggtctgca
     2401 ccacgccctc gaccttgggc agctcctcga gcaccacctt ggtcccgtcc tccggcgtgg
     2461 agcaatcgag ttgcgccacg agcttgtcga aggacagcac caccacattg gcaaacttgc
     2521 aattggcgag actgaggctc ttgatctgat cggccgtgtc ggcggcgctg gcggcacgca
     2581 gtagctcgat aaacagatag atattgacca tcgcgttggc tcctcccggg tctgcgaggc
     2641 cccttatgcc ggggcgcaat ggacagtaaa ctgccgagcc ccttgaggag gttccatgcg
     2701 ccgccttgtc atgctttccg ccgtgttgat cggtttcggc gtgccgccat cgcccgcttc
     2761 cgccttaccc gcgccgatgt cgcaggagca gttgctggag aagagcgacc tcgtggcgct
     2821 ggtccgcatc aagtcggtca cctgcgtcag cgtcgccaag gacgatcgca ccggcgagga
     2881 tttgccgagc tacagcgcca gcgccga
//
LOCUS       DATNAE010000054        10435 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_4271566, whole genome shotgun sequence.
ACCESSION   DATNAE010000054 DATNAE010000000
VERSION     DATNAE010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 10435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10435
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4271566"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="VN518_01020"
     CDS             complement(<1..710)
                     /locus_tag="VN518_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HXE03184.1"
                     /translation="MKAQRVCRVKAIPVAARATRISALWLGVAALTLAVAALPAEAGK
                     KQKPAKAPDESIVTIDNKQPMIVVVSIGQQKVDVYRGTSLVTTSAVSTGTAQHPTFIG
                     AFSIMQKARWHHSNIYSGAPMPWMNRLTWSGTAMHAGVVPGYPASHGCIRLTYAFAPK
                     FFQMSSVGDNVITSRGRPKPTPIEHGALFQPLPPPTLPMVERQTSISPLERISTPIPA
                     VAAASASPVILAKAEMPTT"
     gene            1261..1872
                     /locus_tag="VN518_01025"
     CDS             1261..1872
                     /locus_tag="VN518_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HXE03185.1"
                     /translation="MLALAHPRSRTFVRALALAIAGLALAGCGINTIPTLQERAKASW
                     SEVLNQYQRRADLIPNLVETVKGYAAQESKVLTEVVEARAKATQMQIPPDILTNPEAF
                     KKFQEVQGQLTGSLARLLAVVENYPDLKSNQNFLALQSQLEGTENRIAIARGDYIKAV
                     QAYNTEIRTIPGRWWRAVLYPGAEPMQNFTVAEETTKVPEVKF"
     gene            1898..2677
                     /locus_tag="VN518_01030"
     CDS             1898..2677
                     /locus_tag="VN518_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007560247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HXE03186.1"
                     /translation="MAATPWRRILFGFALIFCIGPAAAAEPVYPQLSGRIVDDADLLS
                     PADRAELDAELKALEDKSSDQVVVVTLPSLQGFTIEDFGYQLGRHWGIGTKEKDNGVL
                     LIVAPNERKVRIEVGRGLEPILTDAMSNVIINGAILPRFRNGDYAGGIKEGAKGIELV
                     LTGDSAELAERVKGRHDADDPKIDWLVVMFWTLIILWFIWVTWRSTQRSAYRRGSGPI
                     FLPGPGWGGGSGGSDWGGGGGGGGFSGGGGSFGGGGASGSW"
     gene            2686..3318
                     /locus_tag="VN518_01035"
     CDS             2686..3318
                     /locus_tag="VN518_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03187.1"
                     /translation="MEPFSPEQRASIEAAITEAESKTSGEIIVVVATASAGYFAYALM
                     WAALLALLVPWPLIYFTNWPVEHIYLAELGVFLLGALLMQWEALRFTIVPSSIKRARA
                     HQKAVEQFLAQNLHTTKGRTGVLIYVSFAEHYAEVIADDGIYKKVPQETWNGVVSTLT
                     THLGRGQRVQGFITTIETCGTILAEQFPPGRIDQNELPNHLIVLDASWTA"
     gene            3299..4705
                     /locus_tag="VN518_01040"
     CDS             3299..4705
                     /locus_tag="VN518_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXE03188.1"
                     /translation="MQAGPRKGKGGVRGKAAERAGPLALTGWVLFDWATQPFYTLVVT
                     FLFGPYFVNGFIRDPALGATLWAYATGGAELVAALLAPVLGSIADAGFARKPWIAGFS
                     VLLVVGLCGLWLAVPGQTGMAPLVLLSFALATIGAELATVFNNAMMPSLVSHKRLGTL
                     SGIGWAAGYVGGLVSLVLVAGFIVADPDTGKTVLGLNPIMPVDAATRADERLVGPFSA
                     LWYLIFVLPLFLFTPDRPGTRAAAPVKTGITQLFAGAKDLYLHHRQIGLFLLARMLYA
                     DGLGAVYAFGGIYAASVFGWGASELGLFGIILTIAGTIGAALGGTLDDHLGSKRVIGW
                     ALLLFILAAIGVLSVDKTHVLFVHAVEPKAPGSAPFSSVGEQVYLAFAILIGLASGPI
                     QAASRTLLARLSPPEKTTEFFGFFSFSGKITAFTAPLTIGAVTSATGSQRLGISTSVL
                     FLVAGLLLLQRVRVKASD"
     gene            complement(4702..6279)
                     /gene="purH"
                     /locus_tag="VN518_01045"
     CDS             complement(4702..6279)
                     /gene="purH"
                     /locus_tag="VN518_01045"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HXE03189.1"
                     /translation="MQKIARALLSVSDKTGLVDFARGLSAQGVELISTGGTAKALRDA
                     GLKVIDVAEITQVPEMMDGRVKTLHPKIHGGLLAVRSKESHEQALKDHDIPKIDLLAV
                     NLYPFESTAGSGADFATCIENIDIGGPALIRAGAKNHASVIVVVDPADYLRVLAEMEK
                     NGGATTPELRKELAAKAFARTGAYDAAIGSWFAETIGNLTPARRVIAGTLADALRYGE
                     NPHQWGAFYRTAEQRFGVATATQVQGKELSYNNLNDADAAYELVAEFDPQATAAVAII
                     KHANPCGVAVAATLKEAYAKALACDPESAFGGVIALNRSLDADAAREIVEIFTEVIIA
                     PDASDEARSIVAGKKNLRLLLAGGSPDPKAPGLTFRSLSGGFLVQARDNGVADADLKI
                     VTKRTPTAAELADMMFAFTVAKHVKSNAIVYAKDGATLGIGAGQMSRVDSARMAAWKG
                     EQAAKAAGLETSLTEGSVAASDAFFPFPDGLQAIAAAGATAVIQPGGAMRDAEVIAAA
                     DEAGLAMVFTGMRHFRH"
     gene            complement(6364..8118)
                     /locus_tag="VN518_01050"
     CDS             complement(6364..8118)
                     /locus_tag="VN518_01050"
                     /inference="COORDINATES: protein motif:HMM:NF019552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HXE03190.1"
                     /translation="MATDPLAGQGRSRAELARGPGPLRRFMLLRMASLSYPGTEAEQL
                     LLAPQDLRTADPSFATEIYNGHFGLAGSLVELGSQSPFEITPPSEGWARELYGFGWLR
                     HLRVAGSELSREQAKALLGDFLKLHKTVRGLAWQPEVVGRRVISWLSNSVVVLDAGNP
                     KSYETFLHSLTAHLRYLSASYRDTPDGAPRLVALMALVYAGLCIAEQQAVVDRYLKPF
                     CKELDRQIMSDGGHISRNPTALVELLLDLLPLRQCFMARDRVPPKQLSDAIDRAMPMV
                     RFFRLGDGTMARFNGGGATATDSLATVLAYDDTGGAPLRAAPNSGYVRLERGTTLITA
                     DVAAAPAGSLSIASHAGCLSFEMSSGEHLVIVNCGAPTPDHDDWRMFARSTSAHSTLT
                     FEETSSAIFSGANGAEPAANAELVGPPNVPTSLNEEDGAFVLKGSHEGYIARFGVAHA
                     RRLELSADGLLVRGEDTLAAPKGLKGEAQTSGGGYAVRFHLHPSVSAEMAEDEKSVRL
                     VLPNREAWTLTANTPTVALEESVFLADERGPRGSFQIVLQGGLEEEREIEVAWTIERT
                     AAPGEASLPQAGDAAAVT"
     gene            complement(8253..9614)
                     /locus_tag="VN518_01055"
     CDS             complement(8253..9614)
                     /locus_tag="VN518_01055"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HXE03191.1"
                     /translation="MSFDEETRDSAAAREAQVGLPARRAAVELLSAVIDKKQPLDDIL
                     SRSLATGWMFDLPARDRALARAIVATSLRRRGEIDRVLDTFLERGMPDKGGKLYPILL
                     SGAAQLVFLKTPPHAAIDLAVRLAQWDSRARRYDKLVNAVLRRVAKEGEAIVASLDAP
                     RVNTPGWLWDRWVARYGEERARAIAAVNLIEPPLDLTVKSDPQHWAIEMSGRVLPNRS
                     VRLLPKGRIEALPGFEQGAWWVQDAAASLPAHLMGGVAGKRVADLCAAPGGKTAQLAL
                     AGASVVAVDVSKTRLKTLSANLARLGLAATTVAADAANWSPEERFDAVLLDAPCSSTG
                     TIRRHPDIPYLKSPKDIEALAALQGRLLDNATALLKPGGTLVYSTCSLELEEGEAQIA
                     ALLARNSGVQVDPILPADVFGQPDWLQSPGFLRTFPYQLDLGTPEWSGMDGFFAARLK
                     RKK"
     gene            complement(9633..>10435)
                     /locus_tag="VN518_01060"
     CDS             complement(9633..>10435)
                     /locus_tag="VN518_01060"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HXE03192.1"
                     /translation="LASALRKISSAAEQIPNPPAERNPASAHLFIVNPLSGARMDNLQ
                     PNAFATGRNPEHAAVAVTTGLLERLNRDELAGVLAHELGHVKHRDTLTMTITATLAGA
                     ISMLANFGFYFGGSRNNDGVGPIGGLLLVILAPIAAALVQFGISRGREYEADRIGAEI
                     SKRPLSLASALRKISSAAEQIPNPPAERNPGSAHLFIVNPLSGARMDNLFSTHPNVEN
                     RIARLDEIAARMGQGAGAETLEPPARASGPWASPQQGGWRRRGPWDQT"
BASE COUNT         1768 a         3369 c         3442 g         1856 t
ORIGIN      
        1 gtggtgggca tctctgcctt cgccagaatg accgggcttg ccgaagctgc ggccacggcc
       61 gggatgggcg tgctgatgcg ctccagcggc gaaatcgagg tctgccgctc gaccatcggc
      121 aaggttggcg gcggcaaggg ctgaaacaac gctccgtgct cgatcggagt gggtttcggc
      181 cggccgcggg aggtgatcac attgtcgccg accgaagaca tctggaagaa cttaggcgca
      241 aacgcatagg tcagccggat gcagccgtgg gaggcggggt aaccgggcac aaccccggca
      301 tgcatcgccg taccagacca ggtaagccgg ttcatccacg gcatcggcgc accgctgtat
      361 atgttggagt gatgccaccg cgccttttgc atgatcgaga aggcaccgat gaaggtcgga
      421 tgctgcgcgg ttccggtcga caccgccgag gtggtgacga ggctcgtgcc gcgataaacg
      481 tcgacctttt gctgcccgat ggagacgaca acgatcatcg gctgcttgtt gtcgatagtg
      541 acgatcgact cgtcgggcgc cttggccggt ttctgcttct ttccggcttc ggcaggcaag
      601 gcggcaaccg ccagggtcag tgccgcaacg cccaaccaca gtgccgaaat tcgtgttgct
      661 cttgcggcga ccgggatcgc ctttacccta cacacacgct gcgctttcaa tgcccgtccc
      721 cgtgctcaat cgaaccctgc ctgggctgaa attgttcccc tcaatcgtgt gactctccct
      781 cgcgaatcac actaggtgag gctatcaaat ttggccagta aagccttccg attacctaac
      841 atttggggcc gctcgtcaaa gatttcacct cgccagcgcg cctgcctcgg ccttgaacac
      901 aactccctgc ctcgcgtggg aaatttcggc ctcatcggca gcgagcccgg ctcatgctgc
      961 agccaagcgc ctcgaataaa cttggccctt gttccattct ggcaacacct agacgctgcc
     1021 gagccacagc cctcgatggc gttcacagtg gaggcagccg catcgcgcgc gagaccggtc
     1081 aaatcgcgcc gtcatttctc ttcaaagtga acctcagatt tgcggtggca cgagggcaga
     1141 aattgtgcct aaccgagatg cgcaaccgga gaccatctcc ggcaaaagcc cttgcttagc
     1201 gagcgcccgc taaatacacc cgatgccgaa agcggctttt ggctcttcag ggaggctgtc
     1261 atgctggcat tggcgcatcc gcgttcccgc acgttcgtcc gtgctctggc tttggcgatc
     1321 gccggcttgg cgctcgccgg ttgcggcatc aacaccatcc ccacgctgca ggagcgggcc
     1381 aaggcctcct ggagcgaggt gctgaaccaa tatcagcgcc gcgccgatct aatccctaat
     1441 ctggtcgaga cggtgaaggg ctatgccgcg caggagagca aggtgctgac cgaggtggtc
     1501 gaggcgcgcg ccaaggcgac gcaaatgcaa atcccgcccg atatcctcac caatcccgaa
     1561 gccttcaaga aattccagga ggtgcaaggc cagctaaccg gatcgctcgc gagactgctg
     1621 gcggtggtgg agaattatcc tgacctgaag tcgaaccaga atttcctcgc gctgcagtct
     1681 cagctcgaag gcaccgaaaa ccgcatcgcc atcgcccggg gcgactacat caaagcggtc
     1741 caggcctaca acaccgagat cagaactatc cccggacgct ggtggcgcgc ggtgctctat
     1801 cccggtgccg agccgatgca gaacttcacc gtcgccgagg agaccaccaa ggtgccggag
     1861 gtgaagttct agggggagcg ggcgaacgag ggcttgtatg gccgcaacgc cgtggcgccg
     1921 gatccttttc ggcttcgcgc tcatcttttg catcggaccc gcggccgcgg cagagcccgt
     1981 ttatccgcag ctctccggcc ggattgtcga cgacgccgat ctgttgagcc ccgccgaccg
     2041 ggccgagctt gatgcggagc tgaaggcgct cgaggacaag agctccgacc aggtggtcgt
     2101 cgtcaccttg ccctcgctgc agggcttcac catcgaggat ttcggctacc agcttggccg
     2161 ccattggggc atcggcacca aggagaagga caacggcgtc ctcctgatcg tcgctcccaa
     2221 tgagcgcaaa gtgcgcatcg aggtcggccg cgggctcgag ccgattctca ccgatgccat
     2281 gtcgaacgtc atcatcaacg gcgcgattct gccgcgcttc cgcaacggcg actatgccgg
     2341 tggcatcaag gagggggcga aaggcatcga gctcgtgctc accggcgatt ctgccgagct
     2401 cgccgagcgc gtgaagggcc ggcacgacgc cgacgatccg aagatcgact ggctcgtggt
     2461 catgttctgg acgctcatca tcctgtggtt catctgggtc acttggcgga gcacccagcg
     2521 ctcggcctat cggcgcggca gcggcccgat ctttctccct ggccccggtt ggggcggcgg
     2581 ctccggcggc agcgattggg gcggcggagg cggcggaggc ggcttctctg gcggaggcgg
     2641 cagcttcggc ggcggcggcg cctcaggcag ctggtaggga tgcccatgga gcccttcagc
     2701 cccgagcaac gcgccagcat cgaagcggcc attaccgagg cagagagcaa aacgagcggc
     2761 gagatcatcg tcgttgtcgc caccgccagc gccggctatt tcgcctacgc gctaatgtgg
     2821 gcggcgctgt tggcgctgct tgttccctgg ccgctgatct atttcaccaa ctggccggtc
     2881 gagcatattt atctcgcaga gctcggcgtc ttccttcttg gcgccctgct catgcagtgg
     2941 gaggcgctac gcttcaccat cgtgccgagc tccatcaagc gcgcccgcgc gcatcagaag
     3001 gcggtcgagc agttcctggc gcagaacctt cacaccacca aaggccgcac cggcgtgctg
     3061 atctacgtgt cctttgccga gcactacgcc gaggtgatcg ccgacgacgg catctacaag
     3121 aaagtgccgc aagagacctg gaatggcgtg gtgagcacgc tgaccacgca tctcgggcga
     3181 ggtcagcgcg tgcaagggtt catcaccacg atcgagacct gcggcacgat tctcgccgag
     3241 cagtttcctc ccggccgcat cgaccagaac gagctgccca accatctgat cgtgctcgat
     3301 gcaagctgga ccgcgtaagg ggaagggcgg cgtcaggggc aaggcggctg aacgcgccgg
     3361 tcctctcgct ctgaccggct gggtgctgtt cgactgggcg acgcagcctt tctataccct
     3421 cgtcgtcacc ttcctgttcg gcccctattt cgtgaacggc tttattcgag accctgcgct
     3481 tggcgccacg ctgtgggcct atgccaccgg tggcgccgag ctggttgcgg cgcttctcgc
     3541 gccggtcctc ggctccatcg ccgatgccgg attcgcgcgc aagccttgga tcgcaggctt
     3601 ctcggtgctg ctggtcgtgg ggctttgcgg gctgtggctc gccgttccgg ggcaaaccgg
     3661 catggcgcct ctcgtgcttc tgtcgtttgc gctcgcaacc atcggggctg agctcgccac
     3721 cgtgttcaac aacgccatga tgccctcgct cgtctctcac aagcgcctcg gcactctgtc
     3781 gggcatcggc tgggcagccg gctatgtcgg cgggctggtg agcctcgtcc tcgtcgcggg
     3841 cttcatcgtc gccgatcccg ataccggcaa gaccgtgctc ggcctcaacc cgatcatgcc
     3901 ggtcgatgcg gcgacgcggg cggacgagcg gctggtcggt ccgttctccg cgctctggta
     3961 cctgatcttc gtcctgccgc tcttcctgtt cacgccggac cggccgggaa cgcgggccgc
     4021 agctccggtc aagaccggaa tcactcagct cttcgcgggc gccaaggacc tgtacctgca
     4081 ccatcgccag atcgggctct ttctcctcgc ccgcatgctt tatgccgacg ggctcggcgc
     4141 cgtgtatgcc tttggcggca tttacgcggc gagcgtgttc ggctggggag cctccgagct
     4201 cggcctgttc ggcatcatcc tcaccatcgc cggcaccatc ggcgcggcgc ttggcggcac
     4261 gctcgacgac cacctcggct cgaagcgcgt gatcgggtgg gcgcttttgc tatttattct
     4321 cgccgcgatc ggcgtgcttt cggtcgacaa gacccacgtg ctgttcgtgc atgccgtcga
     4381 gcccaaggca cctggcagcg cacctttctc ctcggttggc gagcaggtct atctcgcctt
     4441 cgccattctc atcgggcttg cctcgggacc gatccaggcg gcgagccgca cgctcttggc
     4501 gcggctcagc ccgcccgaga agaccacgga gttcttcggc ttcttctcgt tctccggcaa
     4561 gatcacggcc ttcaccgccc cgcttaccat cggcgcggtt acctccgcga ctggcagcca
     4621 gcgcctcggc ataagcacga gcgtgctgtt tctggtcgcg ggattgctgc tgctgcagcg
     4681 tgtgagggtg aaggcctccg actaatgccg gaagtggcgc atgcccgtga acaccatggc
     4741 gagtcccgcc tcgtcggcgg cagcgatcac ctcggcgtcg cgcatcgccc ctccaggctg
     4801 gatcaccgcg gtggcaccgg ccgcggcgat cgcctgcaat ccgtcaggga aggggaagaa
     4861 ggcatcggac gccgccacgg agccttcggt gagcgaggtc tcgaggccgg cagccttcgc
     4921 cgcctgctcg cccttccatg ccgccatgcg ggctgaatcg acgcgggaca tttgcccggc
     4981 gccgatgccg agggtcgcac cgtccttggc atatacgatg gcgttcgatt tcacgtgttt
     5041 cgccacggtg aaggcgaaca tcatatccgc caattccgct gcggtgggcg tccgcttggt
     5101 cacgatcttc aagtccgcgt cggccacgcc gttgtcgcgc gcctgaacga gaaagccgcc
     5161 tgacagcgaa cggaaggtaa ggccgggcgc cttcgggtcg ggcgatccgc cggcaagcag
     5221 cagccgcaga ttcttcttac ccgcgacgat cgaccttgcc tcgtcgctcg catctggtgc
     5281 gatgatcacc tcggtgaaga tctcgacgat ctcccttgct gcatcagcat cgaggctgcg
     5341 gttgagcgcg atcacgccgc cgaacgcgct ttccggatcg caagcgagag ccttggcata
     5401 ggcctctttc agagtggcgg cgaccgccac cccgcaagga ttggcgtgct tgatgatggc
     5461 gacagctgcg gtcgcttgcg gatcgaactc ggcgacaagc tcgtaggccg catcggcatc
     5521 atttaggttg ttgtaggaga gctccttgcc ttgcacctgc gttgccgtgg caacgccgaa
     5581 gcgctgttcg gccgtgcggt agaaggcgcc ccattgatgc gggttctcgc cgtagcggag
     5641 cgcatcggcc aacgtgccgg cgatgacgcg gcgagcggga gtgagattac cgatcgtctc
     5701 ggcgaaccac gagccgatgg cggcgtcgta ggcgccggtg cgggcgaagg ccttagccgc
     5761 gagctctttg cgcaactcag gcgtggtcgc gccgccgttc ttctccatct cagcgagcac
     5821 gcggagataa tcggcggggt cgaccacgac gatgacgctt gcgtgattct tggcgccagc
     5881 gcggatcagc gctggcccgc caatatcgat gttctcgatg caggttgcga aatccgcacc
     5941 gctgcccgcg gtgctctcga agggatagag attgacggcg agaagatcga tcttcggaat
     6001 gtcatggtcc ttgagcgcct gctcgtggct ctccttgctg cgcacggcga gcagcccgcc
     6061 atggatcttc ggatgcagcg tcttcacccg gccatccatc atctcgggaa cttgcgtgat
     6121 ctcggcgacg tcgatcacct taagcccggc gtcgcgcaag gctttggcgg tgcctccggt
     6181 ggagatcagc tcgacgcctt gggcggagag gccccgcgca aaatcgacga gccccgtctt
     6241 gtcgctgacc gaaaggagcg cgcgggcaat tttctgcatg gggcaaatgt cccggggggc
     6301 gaattggcaa gccgcccggt atcacgaatt gccccttcgg gcaaagctgc gtgcggaaca
     6361 agcttacgtg acggcagcgg cgtcgccggc ttgaggcagg ctcgcctcgc cgggtgctgc
     6421 ggtccgttca atcgtccacg cgacctctat ttcccgctct tcttcgaggc caccctgaag
     6481 cacgatctga aagctgccgc gtggcccgcg ctcgtcagca aggaacacgc tttcctcgag
     6541 cgcgaccgtc ggggtgttgg cggtgagcgt ccaggcttcg cggtttggca gcacgagcct
     6601 cacgctcttc tcgtcctcag ccatctcggc gctgacgctc ggatgcagat ggaagcgaac
     6661 cgcatagccg ccgccgctcg tctgcgcctc gcctttgagg cccttgggcg cggcaagcgt
     6721 gtcttcgccg cgcaccagca agccatcggc cgatagctcg aggcggcgcg catgagcaac
     6781 gccgaagcgg gcgatatagc cttcatgcga gcccttgagc acgaacgccc cgtcttcctc
     6841 gttgagcgag gttggcacat tgggaggccc gaccaactcg gcattggcgg cgggctccgc
     6901 gccattcgct ccggagaaaa tcgccgagga ggtctcctcg aaggtgaggg tcgaatgggc
     6961 cgaggtcgag cgggcaaaca tgcgccagtc gtcatggtca ggcgtcggag ccccgcaatt
     7021 gacgatgacg agatgctcgc cagagctcat ctcgaaggag aggcatccgg catgagaggc
     7081 gatgctcaac gacccggcgg gggccgccgc cacatcggcg gtgatgagcg tggttccgcg
     7141 ctccaggcgc acatagccgg agttcggcgc cgcgcgcagc ggcgccccac cggtatcgtc
     7201 ataagcgagc acggtggcga gcgagtcggt ggcggtggca ccgccgccat tgaagcgtgc
     7261 catggtgccg tcgccgaggc ggaagaagcg gaccatgggc atggcgcggt cgatggcgtc
     7321 ggagagctgc ttgggcggca cgcggtcgcg cgccatgaag cattgcctga gcggcaggag
     7381 atcgagcaga agctcgacca gcgccgtcgg gttgcgcgag atgtgaccgc cgtctgacat
     7441 gatctggcgg tcgagctcct tgcagaacgg cttcaggtag cgatccacca ccgcttgctg
     7501 ctcggcgatg cacagcccgg cataaacgag cgccatgagg gcgacgaggc gcggcgcccc
     7561 gtcgggggtg tcgcgatagc tcgccgaaag ataacggagg tgcgccgtga gcgagtgcag
     7621 gaaggtctcg taggacttcg ggttgcccgc atcgagcacc acaacggagt tggacagcca
     7681 ggagatcacc ctccgcccaa ccacctcggg ctgccaggcg aggccgcgca ccgtcttgtg
     7741 cagcttgagg aaatcgccga ggagcgcctt ggcttgctcg cgcgacagct cgctgccggc
     7801 gacccgcaaa tgcctgagcc agccgaagcc gtaaagctcg cgcgcccatc cttcggaagg
     7861 cggcgtgatt tcgaagggcg attgcgagcc aagttcgacc aggctgccgg cgagaccgaa
     7921 atggccgttg tagatctcgg tggcaaaact cggatcggcg gtgcgtaggt cctggggagc
     7981 aagcaggagt tgctcagctt cagtcccagg ataagagagg ctcgccatgc gcaagagcat
     8041 gaagcggcga agggggcccg gcccgcgcgc aagctcagcg cgcgaccggc cctggccggc
     8101 aagggggtca gtcgccatgc tcaatcatct cgaattttgg gccccgcctg caaatcaggc
     8161 gcctcggggc ctccccgcaa aacgtctggg cattgtcgga ctgcagcagg gcaggatcaa
     8221 gaggcttggg gcctaaggcc gcctggaagc tgctattttt tgcgtttcag gcgcgcggcg
     8281 aagaacccgt ccatgccgct ccattcaggc gtcccgaggt cgagctggta gggaaaggtc
     8341 ctcaggaacc ctggggactg tagccaatcg ggctggccaa acacatcagc cggcaggatc
     8401 gggtcgacct gaacgccaga gtttcgggca aggagcgcgg caatctgggc ctctccctcc
     8461 tcaagctcga gcgagcaggt ggaatagacg agcgtcccac ctggcttgag cagggcggtg
     8521 gcattatcga gaagcctccc ttgcaaggcg gccagcgcct cgatgtcctt cggcgacttg
     8581 agatagggaa tgtcaggatg ccggcggatg gtgccggtgg acgagcacgg cgcgtcgagc
     8641 aacaccgcgt cgaagcgctc ctccggcgac cagttggcgg cgtcggcggc gaccgtcgtg
     8701 gcggcaagac cgagcctcgc gagattggcg ctgagagtct tgagccgagt cttggaaacg
     8761 tcgacggcca ccacggaagc gccggcaagc gcaagctgcg ccgtcttgcc tccaggtgcc
     8821 gcgcaaagat cagccacgcg ctttccagcc acgcctccca tgagatgcgc gggcagcgac
     8881 gcggcggcgt cctgcaccca ccacgcgcct tgctcaaaac ctggaagagc ttcgatgcga
     8941 cctttgggca gcaggcgcac gcttcgattg ggaaggacgc ggccggacat ctcgattgcc
     9001 cagtgttgcg gatcgctttt gaccgtcagg tcgagcggcg gctcgatcag attgacggcg
     9061 gcgattgcgc gcgcgcgttc ctcgccgtag cgcgcgaccc accggtccca cagccagccc
     9121 ggcgtgttga cgcgcggcgc atcgaggctt gcgacaatgg cctcgccctc cttggcgacg
     9181 cgccgcagca cggcgttcac gagcttgtcg tagcgccgcg cgcgcgaatc ccattgcgcc
     9241 agccgcacgg caagatcgat cgcggcgtgc ggcggcgtct tcaaaaagac gagctgcgcc
     9301 gcaccggaaa gcaggatggg gtagagcttg ccccccttgt ccggcatccc gcgctcgaga
     9361 aacgtgtcga gcacgcggtc gatctctcca cgacgccgca agctcgtggc cacgatggcg
     9421 cgggcaagcg cccggtcacg cgccggcaag tcgaacatcc atcccgtggc gagcgagcga
     9481 ctcaggatgt catcgagcgg ctgcttcttg tcgatgacgg cggacagcag ctcaacggct
     9541 gcgcgccgcg ccggaaggcc gacctgcgcc tcccgcgcgg cggccgagtc gcgcgtctct
     9601 tcgtcgaagc tcatctgaat ggtcgaggcg gtttaggtct ggtcccaagg accgcgcctc
     9661 cgccagcctc cctgttgcgg cgacgcccag ggccctgacg cgcgtgcggg gggctcgaga
     9721 gtctccgcgc ccgcgccttg acccatgcgc gcggcgatct cgtccagccg ggcgatgcgg
     9781 ttctcgacgt tggggtgggt ggagaacaga ttgtccatcc gcgcgcccga cagcgggttg
     9841 acgatgaaga gatgcgccga ccccggattg cgctcggcgg gcggattggg gatttgctcc
     9901 gccgcgctgg aaatcttgcg caaggccgag gcgagcgaca gcgggcgctt gctgatctcg
     9961 gcgccgatgc ggtctgcctc gtattcgcgg ccccggctga tgccgaattg cacgagcgcc
    10021 gccgcgatcg gcgcgaggat aacgagcaga aggcctccga tcggtccaac gccgtcgttg
    10081 ttgcggctgc cgccgaaata gaagccgaaa ttggcgagca tggagatggc gccggcgagc
    10141 gtcgcggtga tggtcatggt gagcgtgtcg cggtgcttga cgtgaccaag ctcgtgcgcg
    10201 agcacgccgg caagctcgtc gcggttcagc cgctcgagca gcccggtggt gaccgcgacg
    10261 gcggcatgct cgggattgcg cccggtggca aaggcgttcg gctgcagatt gtccatacgc
    10321 gcgcccgaca gcgggttgac gatgaagaga tgcgccgacg ccggattgcg ctcggcgggc
    10381 ggattgggga tttgctccgc cgcgctggaa atcttgcgca aggccgaggc gagcg
//
LOCUS       DATNAE010000055         7430 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2164159, whole genome shotgun sequence.
ACCESSION   DATNAE010000055 DATNAE010000000
VERSION     DATNAE010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 7430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7430
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2164159"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..1248
                     /locus_tag="VN518_01065"
     CDS             67..1248
                     /locus_tag="VN518_01065"
                     /EC_number="2.7.7.60"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase/2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase"
                     /protein_id="HXE03193.1"
                     /translation="MSVTVLIVAAGRGFRASGPKPLIPKQYLPVGGVPMLTRAIAAFA
                     GHPKVDDVIVVIHPEDSGLYEAASQSFSSRLRPPISGGAQRQDSVRSGLEALAESPPE
                     RVLIHDAARPFVDAGLIGRVIASLDSHAGALPCLPVTDTLLRAAAGRATGTLSRDGLW
                     RAQTPQGFRFDAILAAHRAAAKDGALFTDDAGIAQWYGLDVALVEGSEANRKLTSAED
                     IAIADELLRLRAPTSFGTQRVATGYDVHAFGPGDAVILCGVRIPHQKKLVGHSDADVG
                     MHALTDALLGTIADGDIGVHFPPSDARWRGAASELFLKDAAARVKARGGEIVHVDVTF
                     ICEAPRVGPHRDAMRAKLAECLGLDVAEISIKATTNEGLGFIGRGEGIAAMATATVRL
                     P"
     gene            1259..1762
                     /locus_tag="VN518_01070"
     CDS             1259..1762
                     /locus_tag="VN518_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015951272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HXE03194.1"
                     /translation="MIDPLMIDQRIIADAAALLEACRQRGILLATAESCTGGLLAATL
                     TAIPGSSDVVDRAFVTYSNAAKSEMLGVPVWLIGRHGAVSEDVARAMVGGALTHSRAT
                     LAVAVTGIAGPSGGTAEKPVGLVHLAAAVRDAPVSHERLLLGDIGRAEIRRESVRRGL
                     ALLASLL"
     gene            complement(1751..2227)
                     /locus_tag="VN518_01075"
     CDS             complement(1751..2227)
                     /locus_tag="VN518_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HXE03195.1"
                     /translation="MHVYETKHRVAHSADDMFKLVADVELYPKFLPLCEELKLKRRER
                     REGKDVLIATMTVGYKLIRESFTTEVILDADARTIVVHYLDGPFSFLENRWIFRPLTP
                     QACEIDFYIAYRFRSRLLERLMGGLFDRAVRKYTDAFEARADAIHGTRSPSLASQQ"
     gene            complement(2242..3198)
                     /gene="lipA"
                     /locus_tag="VN518_01080"
     CDS             complement(2242..3198)
                     /gene="lipA"
                     /locus_tag="VN518_01080"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012115869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HXE03196.1"
                     /translation="MVTVFTSQDQTRPRHPEKARLPDTPVLPKPDWIRVRAPGPHAFD
                     ETSSVLRRHRLNTVCEEAACPNIGECWGKRHATVMILGDTCTRACAFCNVRTGRPGAV
                     DPSEPARVGAAIAELGLSHVVVTSVDRDDLADGGAAHFAATIAAIRTKAPATTVEVLT
                     PDFLRKEGALDIVMAARPDVFNHNLETVPSLYREIRPGARYDHSLRLLERAKELDGVT
                     FTKSGIMVGLGETTAEVLALMDDLRAAQVDFLTIGQYLQPTRKHAAIDRFVPPEEFDA
                     LRAAGEAKGFLLVAASPLTRSSYHAADDFALLKAARHAALGR"
     gene            complement(3199..3486)
                     /locus_tag="VN518_01085"
     CDS             complement(3199..3486)
                     /locus_tag="VN518_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008838088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HXE03197.1"
                     /translation="MENEAHGLLSMPGVGFFGMLVIGILAGYIAEKVTASDHGLLTNL
                     LVGIAGSFVGGSLATLLNIAFYGWLGNLIVATIGAILLLWVWRRFRRPSTA"
     gene            complement(3596..3847)
                     /locus_tag="VN518_01090"
     CDS             complement(3596..3847)
                     /locus_tag="VN518_01090"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HXE03198.1"
                     /translation="MTMIFDLIMFLIIGVAAGAIASFYMGRQQELVPNLLIGIVGAFI
                     GGFIAKLLGLTAYNVIGEVVVATAGAIICLFVWQRIRGK"
     gene            complement(3910..5316)
                     /gene="lpdA"
                     /locus_tag="VN518_01095"
     CDS             complement(3910..5316)
                     /gene="lpdA"
                     /locus_tag="VN518_01095"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HXE03199.1"
                     /translation="MESAESQFDLAVIGAGPGGYVAAIRAAQLGLKTAIVEREHLGGI
                     CLNWGCIPTKALLRTAELYHSMGRATEFGLACDNLRFDLASVVERSRKVAARLSNGVA
                     YLMKKNKITVIDGEATLKGEGKVAIAGKDGAARTIDAKHIVIATGARARELPGLEADG
                     RLVWTYKHAMVPPAFPKSLLVIGSGAIGIEFASFYRTLGAEVTVVELLDRILPVEDAE
                     ISALARKAFSKQGMTIHTGTGVEGLARHEDSVTARLAKPGLTGEITFDRVILAIGITG
                     NVEGLGLEQQGVKIEKGHIVVDEWGATGIPGLYAIGDVVSPPWLAHKASHEGVLCVEK
                     IAGVEGVHPLDKSAIPACTYSAPQVASVGMTEEAAQAEGRSVKVGRFPYSANGKAIVL
                     GETEGLIKTIFDAESGELLGAHMIGAEVTELIQGFAVAKGLETTEAELIASIFPHPTL
                     SEMMYESVLDAYGRALHI"
     gene            complement(5322..5933)
                     /locus_tag="VN518_01100"
     CDS             complement(5322..5933)
                     /locus_tag="VN518_01100"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXE03200.1"
                     /translation="MEGELTFRPATKADASALAVLVDIAGEGLPAHLWSTLKVPGQSI
                     LEVGRERAAREQGGFSYRNAIVAEVGGEIAACLVGYRLDDPYDPGNLAEIPELMRPLV
                     LLEAKAPGSWYVNVLATFHEFRRQGIGVALLAIAEQKALADGAGALSVIVAGGNERAA
                     RLYRRAGFAAAAREPLVPFPGYTHEGDWVLLIKRRGGSAESGR"
     gene            complement(5933..6958)
                     /locus_tag="VN518_01105"
     CDS             complement(5933..6958)
                     /locus_tag="VN518_01105"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HXE03201.1"
                     /translation="MSIRGANLHSGEGGHANRDHAKRVLASPLARRLARQRGIDLGKL
                     RGSGPKGRIVKRDIPPAADARIPEQAEAIEAIEARPEPERRLTLSRPAPAAGLTDKQV
                     LALYEPGSYELVPHDTMRRVIAQRLTLAKQTIPHFYLGIDCELDAVLAARKRLNGMAP
                     QDGPRSFKLSVNDFIIKALAMALQKVPRANASWTEEGLLLHRGSDIAVAVALPGGGLF
                     TPVVRNAELKSLSEISNEMKDLAGRARSKRLAPHEYQGGTTTISNLGMYGIDRFDAVI
                     NPPQASILAVGRAEKRPVIKDEAVKIATMMSATLSVDHRVIDGALGAELLAAFKAFIE
                     DPVTMLV"
     gene            complement(6962..7363)
                     /locus_tag="VN518_01110"
     CDS             complement(6962..7363)
                     /locus_tag="VN518_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HXE03202.1"
                     /translation="MTEAPMTWKSGGCHCGAVRYEVLAPDEIEVKECNCSICRKSGYL
                     HLMVEAERFRLLKGADKITTYSFNTGTAKHHFCSVCGVKSFYVPRSKPGGFSVNVRCL
                     DGGAVRVTRIMMFDGEHWEEAMAKLAAAREG"
BASE COUNT         1254 a         2379 c         2463 g         1334 t
ORIGIN      
        1 cgcagtagca actagacttt tgccgcagtg cacaaaactg tccagactac ttatgggtac
       61 ctctccatga gcgttaccgt cttgatcgtg gcagcggggc gcgggttcag ggcctctggg
      121 cctaagccac tgatcccaaa acaatatctt ccggtcggcg gcgtgccgat gttgacccgg
      181 gcgatcgccg cctttgccgg gcatcctaag gtggacgacg tgatcgtggt gattcaccct
      241 gaggattccg ggctgtacga ggccgcctcg cagagttttt cttcgcggtt gcggccaccg
      301 atctcaggcg gtgcgcagcg ccaggactcc gtccgctctg ggcttgaggc gcttgccgaa
      361 agcccgcccg agcgcgtgct gatccatgac gccgcgcgac ccttcgtcga tgcgggattg
      421 atcggccgcg tcatcgcgag cctggacagt catgccggcg cgctaccctg ccttccggtg
      481 acggatacct tgctgcgggc agccgctggc cgcgcgaccg ggaccctttc ccgcgatgga
      541 ttgtggcgcg cgcagacgcc gcaaggcttc cgcttcgatg cgatccttgc cgcccatcgc
      601 gccgcggcaa aagacggcgc cctcttcacc gacgatgccg gcatcgcgca atggtacggt
      661 ctcgatgtcg cgctggtcga aggctctgaa gccaatcgca agctcacttc ggcagaggac
      721 atcgcgatcg ccgatgagct cttgcggctt cgggcgccga ccagtttcgg cacgcagcgc
      781 gtggcgaccg gctatgacgt gcatgccttc ggtccgggcg acgcggtgat cctgtgcggc
      841 gtgcgcattc cgcaccagaa gaaactcgtg ggccatagcg acgccgatgt cggcatgcat
      901 gcgctgaccg acgctctgct cggcacgatc gccgacggcg atatcggcgt tcacttcccg
      961 ccgtcggatg cgcgctggcg gggcgcagcc tcggagctgt ttctcaaaga cgccgcggcc
     1021 agggtcaaag cgcgcggcgg cgagatcgtg catgtcgatg tgaccttcat ctgcgaggca
     1081 ccacgcgtcg ggcctcatcg cgatgcgatg cgggcgaagc tcgccgagtg cctcggcctc
     1141 gatgtcgcgg agatcagcat caaggccacc accaatgagg gtctcggctt catcgggcgt
     1201 ggcgaaggga tcgcggccat ggccaccgcc accgtgcgcc tcccatgacg gatcgccgat
     1261 gatcgacccg ctcatgatcg accagcggat cattgccgac gccgcggcgt tgctcgaggc
     1321 ttgccgccaa cgcgggatct tgctcgccac cgcagagtcg tgcacaggcg gcctgctcgc
     1381 cgccacgctc accgccattc ccgggtcctc agatgtggtc gatcgcgcct tcgtcactta
     1441 ttcgaacgcg gcgaaaagcg agatgctcgg cgttcccgtt tggcttatcg ggcggcacgg
     1501 cgcggtgagc gaggacgtcg cccgcgccat ggtcggcggc gcgctcactc attcccgcgc
     1561 gacgctcgcg gtcgccgtca ccggcatcgc cgggccttcg ggcgggacgg cggagaagcc
     1621 ggtcgggctc gtgcaccttg cggcggcggt gcgcgacgcg ccggtcagcc acgagcggct
     1681 tctgctcggc gatatcggcc gggccgagat caggcgcgaa agcgtgcggc gcgggctggc
     1741 acttctggcc tcattgctgt gaagcaagcg agggggagcg ggtgccgtga atcgcgtcgg
     1801 cacgcgcttc gaaggcgtcg gtgtatttgc gcaccgccct gtcgaacagc ccgcccatca
     1861 gccgctccaa gaggcgcgag cgaaatctgt aggcgatgta aaaatcgatc tcgcaagctt
     1921 gcggtgtgag cgggcggaag atccagcggt tctccaggaa cgagaacggt ccgtcgagat
     1981 aatgcaccac gatggtgcgg gcgtcggcgt cgaggatgac ttcggtggtg aagctctcgc
     2041 ggatcagctt gtagccgacc gtcatggtgg cgatgagcac gtccttgccc tcacggcgtt
     2101 cgcggcgctt gagcttcagc tcctcgcaga gcggcaggaa tttcggatag agctcaacat
     2161 cggcaacgag cttgaacatg tcgtcggcag aatgagcgac acggtgcttg gtctcataga
     2221 cgtgcatgga agctggcgcg cctagcggcc aagcgctgcg tggcgagccg ctttgaggag
     2281 ggcgaagtcg tcagcggcgt ggtaggagga gcgcgtcaac gggctcgccg ccacgagcag
     2341 gaagcccttg gcttctcccg cagcgcgcaa agcatcgaac tcctctggcg gcacgaagcg
     2401 atcaatggcc gcatgcttgc gggtcggctg cagatattgg ccgatggtga ggaagtcgac
     2461 ctgagccgcg cgcagatcgt ccatcagggc aaggacttcg gccgtggtct cgccgaggcc
     2521 gaccatgatg cctgacttgg tgaacgtgac tccgtccagc tccttggcgc gctcaagcag
     2581 gcgtaaggag tggtcgtagc gcgcgccggg tctgatctcg cgatagagcg acggcacggt
     2641 ttcgagattg tggttgaaca cgtcgggccg cgccgccatg acgatgtcga gcgctccctc
     2701 tttgcgcaag aagtccgggg tcagcacctc gacggtcgtt gccggtgcct tggtcctgat
     2761 ggcggcgatg gtggcggcaa aatgcgcagc accgccgtcg gcaagatcgt cgcgatcgac
     2821 cgacgtcacc accacatggg aaagaccaag ctcggcgatt gcggcgccga cgcgtgcggg
     2881 ctcggaaggg tcgaccgcgc ctgggcgtcc ggtgcgcaca ttgcaaaagg cgcaagccct
     2941 ggtgcaggtg tcgccgagga tcatcacggt cgcgtgccgc ttgccccagc actcgccgat
     3001 attgggacag gccgcctcct cgcacacggt gttgagacga tggcggcgca agacgctgct
     3061 cgtttcgtcg aaagcatgag gccccggcgc tcggacgcgg atccaatcgg gcttgggtag
     3121 caccggcgta tccggcagcc gggccttttc cggatggcgc ggccgcgtct gatcttggct
     3181 ggtgaaaacg gttaccatct acgctgtcga cggtctcctg aacctccgcc acacccacaa
     3241 gagaaggatc gccccgattg tggccacgat cagattgccg agccagccat agaaggcgat
     3301 gttgagcaga gtggcgagcg agccgccgac gaaggagccg gcaatgccga cgagcaaatt
     3361 ggtgagcagg ccatgatcac tcgccgtcac cttctcggcg atgtagccgg cgaggatgcc
     3421 gatgacgagc atgccgaaaa agccgacccc cggcatggac aggagtccat gagcctcatt
     3481 ttccatttcg aacctccgtc ggttgaaacg agctgccccg cctttgaccg gcccccgcga
     3541 ctctttgggc ctcgcgcgta atataggggg tctccccgct cccgcaacga gcggatcact
     3601 ttccgcggat gcgctgccac acgaacaggc agatgatggc gccggcggtg gcgacgacca
     3661 cctcgccgat gacgttatag gcggtgagcc cgagcagctt ggcgatgaag ccgccgatga
     3721 aggcgccgac gatgccgatg aggaggttcg gcacaagctc ctgctggcgc cccatgtaga
     3781 aggaagcgat ggcaccggcg gccacaccga tgatgagaaa catgatgagg tcgaaaatca
     3841 ttgtcatgtc gctgctcctg tcttaggcgc cgggtcgagc ccgggtgttc gacggctggc
     3901 ttgagcggtt cagatgtgaa gcgcgcggcc ataggcgtcg agcaccgact catacatcat
     3961 ttccgacagc gtcggatggg ggaagatgga cgcaatcagc tcggcctcgg tggtttccag
     4021 ccctttggcg acggcgaagc cctgaatgag ctcggtgacc tcggccccga tcatgtgcgc
     4081 gccgagaagc tctccgctct cggcatcgaa gatggtcttg atcagccctt cggtctcgcc
     4141 gaggacgatg gccttgccgt tggcggaata aggaaagcgc ccgaccttga cggatcgacc
     4201 ttcagcttgc gccgcctcct cggtcatgcc gacgctcgca acttgcgggg cgctataggt
     4261 gcaggccggg atggcgctct tgtcgagcgg gtgaacgcct tcgacgccgg cgatcttctc
     4321 cacgcacagc acgccttcgt ggctcgcttt gtgcgcaagc caaggagggc tgaccacgtc
     4381 gccgatggca tagaggccgg ggatgccggt ggcaccccat tcgtcgacca cgatgtggcc
     4441 cttctcgatc ttgacgccct gctgttcgag gccgagacct tcgacattgc cggtgatgcc
     4501 gatggcgagg atgacgcggt cgaaagtgat ctcgccggtg agcccaggtt tggccaaccg
     4561 tgccgtgacg ctgtcttcgt ggcgcgcgag gccctccacg ccggtcccgg tatggatggt
     4621 catgccctgc ttggagaagg ctttgcgcgc aagggcggag atctcggcat cctcgacagg
     4681 aaggatgcgg tcgagcagct cgaccacggt cacttcggcg ccaagcgtgc ggtagaagct
     4741 tgcgaactcg atgccgatgg cgccggagcc gatcaccagc aacgatttgg gaaaggccgg
     4801 aggcaccatg gcgtgcttgt aggtccacac cagcctgccg tcggcctcaa gccccggcag
     4861 ctcccgcgcc ctggcgccgg tcgctatcac gatatgtttg gcgtcgatgg tgcgcgccgc
     4921 gccgtccttg ccggcgatcg ctactttccc ctcacccttc agcgtggcct ctccgtcgat
     4981 caccgtgatc ttattcttct tcatcaggta ggcgacgccg tttgacagcc gcgccgccac
     5041 tttgcggctc cgctcaacca cgctggcgag gtcgaagcgg agattgtcgc aagcaagccc
     5101 gaactccgtg gcgcggccca tggagtgata gagctcggcg gtgcgaagca gcgccttggt
     5161 cgggatgcat ccccaattga ggcagatgcc gccgagatgc tcgcgctcga cgatcgccgt
     5221 cttgagcccg agctgggccg cgcggatcgc cgccacatag cctccgggtc cggcgccgat
     5281 cacggcaaga tcgaattggc tctcagcgct ctccatgctc atcatcgccc gctttcggcg
     5341 gagcctcctc gtcgctttat caacaggacc caatcgcctt cgtgagtgta gccgggaaag
     5401 ggaaccagag gctcccgcgc ggccgcggca aaacctgcgc gccggtagag acgcgcggcc
     5461 cgctcgtttc cgccggcaac gatcacgctc aaagcgcccg ctccgtctgc tagcgccttc
     5521 tgctcggcga tggcaagcaa tgccacgccg atcccttgcc gtcggaactc gtggaaggtc
     5581 gcgagcacat tgacgtacca tgagccaggc gctttcgcct cgagtaagac gagaggccgc
     5641 atgagctccg gtatctccgc caaattgccg gggtcgtagg gatcgtccag ccggtagccg
     5701 acgaggcaag cggcgatctc gcccccgacc tcggcgacga tggcgttgcg ataggagaag
     5761 ccgccctgtt cccgcgcggc gcgctcgcgc ccgacctcga ggatcgattg gccagggact
     5821 ttcaacgtgc tccacaaatg cgcgggcaga ccctcaccgg cgatatcgac cagcacggca
     5881 agcgctgagg cgtcggcttt ggtcgctggc ctgaaggtaa gctcgccttc catcacacca
     5941 gcatggtcac cgggtcttcg atgaaggcct tgaaggcggc aagaagctcg gcgccgagcg
     6001 cgccgtcgat cacccggtga tcgaccgaca atgtcgcgct catcatggtg gcgatcttca
     6061 ccgcttcgtc cttgatcaca ggccgcttct cggcgcggcc gacggcgagg atgctcgctt
     6121 gcggcggatt gatcacggcg tcgaagcggt cgatgccgta catgccgaga ttggagatcg
     6181 tggtggtgcc gccctgatat tcgtgagggg cgaggcgctt cgaccgggcc cgccccgcga
     6241 ggtccttcat ctcgttggaa atttccgaca ggcttttcag ctcggcattg cgaaccacgg
     6301 gtgtgaacag gccgccaccc ggtagggcca cggcgacggc gatgtcggag ccgcgatgca
     6361 ggagcagccc ctcttccgtc cagctcgcat tggcgcgcgg cactttctgc aacgccatgg
     6421 cgagcgcctt gatgatgaag tcgttgaccg agagcttgaa ggagcgtggg ccgtcttgcg
     6481 gcgccatgcc gttgaggcgc ttgcgcgcgg caagcacggc atcgagctcg cagtcgatgc
     6541 cgagatagaa atgcgggatg gtttgcttgg cgagcgtgag gcgctgggca atgacccggc
     6601 gcatggtgtc gtgcggtaca agctcgtagc tgcctggctc gtagagcgcc agcacctgct
     6661 tgtcggtcag cccggcggcc ggcgccggtc gcgacagcgt gaggcgccgc tcaggctcgg
     6721 gcctcgcctc gatggcttca atcgcttccg cttgttcggg gatgcgcgca tcggcggcgg
     6781 gagggatgtc gcgcttgacg atgcgcccct ttggacctga gcctcggagc ttgccgaggt
     6841 cgatgccgcg ctggcgggca agcctccggg caagcggcga agcgaggaca cgcttcgcgt
     6901 ggtcgcggtt cgcgtggcca ccttctccac tgtggaggtt tgcgcctcgt atggacataa
     6961 atcagccttc cctcgccgcg gcgagcttgg ccattgcctc ttcccagtgc tcgccgtcga
     7021 acatcatgat gcgggtcacg cgcaccgcgc cgccgtcaag gcagcgcaca ttgacgctga
     7081 agccgcccgg cttggagcgc ggcacgtaga aactcttgac gccgcacacc gagcagaaat
     7141 ggtgtttcgc cgtgccggtg ttgaaactgt aggtggtgat cttgtcggcg cccttgagca
     7201 ggcgaaagcg ctccgcttcc accatcagat gcaggtaacc cgacttccgg cagatcgagc
     7261 aattgcactc cttgacctcg atctcgtcgg gcgcgagcac ctcgtagcgc accgcgccgc
     7321 aatggcagcc gccgctcttc caggtcatgg gggcttcggt catctcgcgc tactcccggt
     7381 agcagacgga tttcgcggcc gcgaccacct gctgcaccga cggaagcgcc
//
LOCUS       DATNAE010000056         5838 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3973985, whole genome shotgun sequence.
ACCESSION   DATNAE010000056 DATNAE010000000
VERSION     DATNAE010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5838
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3973985"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VN518_01115"
     CDS             <1..471
                     /locus_tag="VN518_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HXE03203.1"
                     /translation="GEEGVMAKFARGHYYSRQRTREWLKVKASLGQEVVIVGFTAPRR
                     SRAYFGSLVLAVRESDAWRYVGRAGTGFDRESLKSLHALMVPLITKTKPVAEKVPDEA
                     NTTWVKPKLVGEVKFTEWTSKGEMRHPVFLGVRTDKKATDVIREMPKPGSAPAH"
     gene            870..1025
                     /locus_tag="VN518_01120"
     CDS             870..1025
                     /locus_tag="VN518_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03204.1"
                     /translation="MHSSLDASMFVVGTETDHIAPWRSVYKLQLFTDNDLTFLLTNGG
                     AQRRDRV"
     gene            complement(1253..2785)
                     /locus_tag="VN518_01125"
     CDS             complement(1253..2785)
                     /locus_tag="VN518_01125"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HXE03205.1"
                     /translation="MSDSDKAKGEPREFELKLEFDPADLAAIEAHPLLAGIRLVRQTL
                     VSVYYDTDTRALHEAGLCLRVRNTGKGYVQTIKGMNGTAELFDRPEWEREVSGPRPDL
                     KLAAGTPLEPLLKDGMGEPLRPLFQTSIERGVYHVASGGSDIEVAVDRGAIETAQRQA
                     PVHEVELELMRGDPAELFRLARSLAATVPLRLAVKTKAERGYELAGNAAPGAEKAGRL
                     DLDGELSCQEAFRAVAQSCLRQIIVNEPGVCAGDAPSLHQMRIGLRRLRAAIVAFETI
                     AADAEQDRIRDELKWITNELGPARDLDVFEADILQPMSKTRGDDARLAEARRAFTTAR
                     AKAYRAAAQSVRSDRFRNAVLDVAEWIEAGSWTLDPDLRERREEAIAEHAAELLSRRR
                     KRIREKGADLRALDIKRRHKLRIRAKNLRYAVEFFAGVFPGEENAKRREAALAELKEL
                     QDQLGALNDLAQREALAANGDDLGEHAQGLLRPKEVDVDQLLDRAQKAHADFAKVKSF
                     WK"
     gene            complement(2947..4179)
                     /locus_tag="VN518_01130"
     CDS             complement(2947..4179)
                     /locus_tag="VN518_01130"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HXE03206.1"
                     /translation="METRARYALIGAFMLAVIAASFAFVYWLENKGGFGQRAYYQLRF
                     DSSVSGLQEGSAVLFNGIRVGEVTGLTLDPEHPDQVVATVGVVNGTPVRSDTKVGIES
                     QGLTGGAAVTLTGGDASSPPVSPENGELPVLSAEPGAGQDWQSLGREALRKLNNILSD
                     NADNLHSAIANFNTFTDVLAKNSDRIEGLLAGLEKLTGGGSSKTAAPTYDVNAVKDIS
                     KPTEMPSWQLVVPEPTALLALNSDKIKVRPAEGAESTDISDARWSDNIPILLQEKFIQ
                     SFENAGYSQAVSRPRDGFDAAYQLLLDVRGFSLVTGAEPHGELEFEAKILGPGGKIIA
                     AKSFHTTAPASGTDAAAAAAALNQAFAEAAKELVPWTLDVVNSAPPEPAPPPAGQAEP
                     ESEAPAPSGEPPAPSPPT"
     gene            complement(4189..4971)
                     /locus_tag="VN518_01135"
     CDS             complement(4189..4971)
                     /locus_tag="VN518_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXE03207.1"
                     /translation="MKDKPVISIKDLVVGFGDQTVLDHLSLDVRKGEILGVVGKSGGG
                     KSVLLRTIIGLLPKWHGKIEVLGVDLDTASEQQRRALERRWGVLFQQGALFSSLTVRQ
                     NVQFPMRENLEISQRLMDEMALAKLDMVGLTPVDADKYPAELSGGMTKRAALARALAL
                     DPEIVFLDEPTSGLDPIAAGDFDALIKTLQGTLGLTVYMNTHDLESLYTVCDRIAALA
                     DGRIVALGPMETMLECTHPWVQTYFQGKRGGMLTKRIGQLVD"
     gene            complement(4982..>5838)
                     /locus_tag="VN518_01140"
     CDS             complement(4982..>5838)
                     /locus_tag="VN518_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXE03208.1"
                     /translation="EEVRQVNRHTPAPVAVPNPVLLKLNDLGKATIEAREDISIFLQM
                     LGALFMAVIGVVRRPRSLRLTSLVYQLNRIGLQAIPIVVLITFLIGAIIAQQGFFHFR
                     RFGAESYTVDMVGILVLRELGVLIVAIMVAGRSGSSYTAELGSMMMREEVDALRTMGF
                     DPVEVLILPRVLVLIIALPVLTFLGSMAALYGGGVVAWLYGGMSPEIYISRLEDAISL
                     THFKVGMVKAPFMGLVIGVVACAEGLLVKGSAESLGLQTTASVVKSIFLVIVLDGLFA
                     IFFASIGM"
BASE COUNT         1091 a         1887 c         1807 g         1053 t
ORIGIN      
        1 ggcgaagaag gcgtcatggc aaagtttgcc cgcggtcatt actactcacg ccagcgcacg
       61 cgggaatggc tcaaggtcaa agcctcgctt ggccaggagg tggtcattgt cggcttcacc
      121 gcgccgcggc ggtcgcgggc atatttcggc tcgctcgtgc ttgccgtgcg cgagagcgat
      181 gcctggcgct atgtcggccg cgccggaacc ggcttcgacc gcgagtcgct caaatccctg
      241 cacgcgctga tggtgccgct catcaccaag acgaagcccg tcgccgaaaa ggtcccggac
      301 gaggcgaaca ccacctgggt caagcccaag cttgtcggcg aggtgaagtt caccgaatgg
      361 acctcgaaag gcgagatgcg ccatccggtc ttcctcgggg ttcgcaccga caagaaggcg
      421 accgacgtga tcagggagat gccgaagcca gggtccgctc ccgcccattg atcgaggtca
      481 aggactgggc tgaattgccg cttcactcca aggcgttatc tcgcgttatg gaacggtcat
      541 aaaactcgct catcaattca gcctggggcc cgcggccccc cgctcacaat cggcttgacc
      601 aaagatggac gaaattctca ggctggtcca agcctcgcca ggcggcgagg cacaggcgag
      661 gcctgaaccg tacgcggcaa gcgacgcgac gcgcctaccc tccttcgaga caatggaccg
      721 cgtcggccgc gccatgacgg cgagaatgac ccaaggcatt tcgccgcaag cgatctatgc
      781 cgcttggttc gactgggcct cacacctcgc gaggtctccc ggcaggctga tcgacttcgg
      841 cctggaggcg gcgaatctcg gcgctcgctt tgcacagttc gctcgacgcc tcgatgttcg
      901 tggtcggcac cgagaccgat cacatcgcgc cttggcgctc ggtctacaag ttgcagctct
      961 tcaccgataa tgacctcacc ttcctgctca ccaatggggg ggcacaacgc cgggatcgtg
     1021 tctgagcctg gtcatcacgg gcgccattac gtcatggcca ctcgcaaccg cggggaacgc
     1081 tacatcgatt ccgacacctg gctcacccgc gccgcccacg gtgatggctc gtggtggccg
     1141 gcttggatgg cctggctcga ggcgaggagc gacagggagc atgtatcggc gctccctctc
     1201 gcgggctcat gccgctttgc ccggcgcccg gcgtctacgt ccatagtgct agttatttcc
     1261 agaaggattt caccttggcg aagtcggcat gcgccttttg ggcgcggtcg agcaattgat
     1321 cgacgtcgac ctctttcggc ctcaacaacc cttgcgcatg ttccccgagg tcgtcgccat
     1381 tggcggcgag cgcctcgcgc tgcgcaagat cgttcagcgc gccgagctga tcctgcagct
     1441 ccttcagctc ggccaatgcc gcctcgcgcc gcttggcgtt ctcctcgccg ggaaaaacgc
     1501 cggcgaagaa ctcgaccgcg tagcgcaaat tcttcgccct gatgcgcaac ttgtggcgcc
     1561 ttttgatatc gagcgcgcgc aaatctgcac ccttctcgcg gatccgtttt cgcctgcgag
     1621 agagcaattc ggcggcatgc tcggcgatcg cctcctcgcg gcgctcgcgc aagtcagggt
     1681 cgagcgtcca cgatcccgcc tcgatccact cggcgacatc gagcaccgca ttgcgaaacc
     1741 ggtcggagcg gaccgattgg gccgccgccc tgtaggcttt ggcacgggcg gtggtgaagg
     1801 cgcgacgggc ctcggccaag cgcgcatcgt cgccacgggt tttactcatt ggctgcaaga
     1861 tgtcggcttc aaagacgtcg agatcacgcg ccggtccgag ctcattcgtg atccatttca
     1921 actcgtccct gatcctgtcc tgctctgcat cggcagcgat cgtctcgaag gcgacgatgg
     1981 cggcacgcag ccgccgcagg ccgatgcgca tttggtgcaa ggatggggca tcgcccgcgc
     2041 acacgccggg ctcattgacg atgatctggc ggagacagct ttgcgcgacg gcgcggaagg
     2101 cctcctggca ggagagctcg ccgtcgagat cgagacgccc agccttctcg gcgccaggcg
     2161 cggcattccc ggcaagctcg tagccgcgct cggctttcgt cttcaccgcg agccgcaatg
     2221 ggacggttgc ggcgaggctg cgcgcgaggc gaaagagctc ggccggatcg cctctcatca
     2281 gctcgagctc gacctcgtgc acgggagcct gacgctgtgc cgtctcgatc gcgccgcgat
     2341 cgacggcaac ttcaatatcg gacccgccgc ttgcgacatg atagacgcct cgttcgatgc
     2401 tggtctgaaa cagcggccgc aagggctcgc ccatgccgtc tttcaacagc ggctcgagcg
     2461 gcgtgccggc ggcgagtttg agatcaggcc gaggaccgct gacctcccgc tcccattccg
     2521 ggcggtcgaa cagctctgcc gtgccgttca tgcccttgat ggtctgcaca tagcctttgc
     2581 cggtattgcg cacgcgcagg caaagacccg cctcgtgcaa ggcacgggtg tcagtgtcgt
     2641 aatagaccga gaccagggtc tgccggacga gccttattcc ggcaagcaga ggatgcgctt
     2701 cgatagcggc aagatcggct ggatcgaatt caagtttaag ctcgaactct cttggctcac
     2761 ctttggcctt atcactatcc gacataaccg caactcttac gcaccaagcc aagtgagcca
     2821 gaaggcgttg acactatcat gtctgaagaa gctcaatctt gcgggaaaag tgtcatggaa
     2881 ctgtcacaaa atgacaccta ccggggaggg acgccggctc ttcgaggtcg ttggcttcgg
     2941 aagagtctag gtcggaggcg agggcgccgg cggttcgcct gaaggcgctg gcgcctcgga
     3001 ttccggctcg gcctgccctg cgggaggcgg agcgggctct ggtggagcgc tattgacgac
     3061 gtcgagggtc cacggcacga gctcctttgc ggcctcggcg aaggcctggt tcaacgccgc
     3121 cgccgcagca gcagcatcgg tgccgctcgc cggcgccgtc gtgtggaacg acttcgccgc
     3181 gatgatcttg ccgcccggtc cgaggatctt cgcctcgaat tcgagctcgc catgaggctc
     3241 cgcgccggtg acgagcgaga agccccgcac gtcgagcaga agctgatagg ccgcgtcgaa
     3301 tccgtcacgc ggccggctca cggcttgcga atagccagcg ttctcgaagc tctggatgaa
     3361 cttctcctga agcaggatcg ggatattgtc ggaccatctc gcatccgaaa tgtcggtgct
     3421 ctccgcaccc tcggcaggac gcaccttgat cttgtccgag ttcagcgcga gcagcgccgt
     3481 gggctcaggc accacgagct gccaggacgg catttctgta ggcttggaaa tgtctttcac
     3541 cgcgttgacg tcgtaagtcg gcgcggccgt ttttgacgag cctcctcccg tcaacttctc
     3601 gagcccggcg agcagaccct cgatgcgatc agagttcttc gccagcacgt cggtgaaggt
     3661 gttgaagttg gcgatggcgc tgtgcagatt gtcggcattg tcggagagaa tgttattgag
     3721 cttgcgcagc gcctcgcggc caagcgactg ccagtcctgc ccggcaccag gctcggcgct
     3781 gagcaccggc agctcgccat tctcgggcga gaccggcgga cttgaagcat cgccaccggt
     3841 gagcgtcacc gccgcgccgc cggtcaagcc ttgcgactcg atcccaacct tcgtgtcgct
     3901 gcgcaccggc gtgccgttga ccacgccgac ggtggcgacc acctgatcgg gatgttcagg
     3961 atcgagcgta agccctgtca cctcgcccac gcgaatgccg ttgaacagta ccgccgagcc
     4021 ctcctgaagc cccgacaccg agctgtcgaa gcggagctgg taataagcgc gttggccgaa
     4081 tccgcccttg ttctccagcc aatagacaaa ggcgaagctc gccgcgatca cggcgagcat
     4141 gaaggcgcca atcagtgcgt agcgggcacg ggtttccatg ttcgagcctc aatccaccag
     4201 ctggccgatt cgtttcgtga gcattccgcc gcgcttccct tgaaaatagg tctgcaccca
     4261 gggatgcgtg cattcgagca tggtctccat cggtcccaag gcaacaatgc ggccgtcggc
     4321 gagcgcggcg atgcggtcgc acacggtata caggctctcg aggtcgtggg tgttcatgta
     4381 cacggtgaga ccgagcgttc cttgcagcgt cttgatcagc gcgtcgaaat ctcctgccgc
     4441 gatcggatca agtcccgacg tcggttcatc cagaaaaacg atttcggggt caagcgcgag
     4501 cgcgcgggca agtgctgcgc gtttggtcat gccgcccgac aactctgccg ggtatttgtc
     4561 ggcatcgacg ggcgtgagcc caaccatatc gagcttggca agcgccatct cgtccatcag
     4621 ccgctgcgag atttcgagat tctcgcgcat gggaaactgc acgttctgcc gcacggtgag
     4681 cgaggagaac aacgcaccct gctggaacag cacgccccag cggcgctcga gcgcacgccg
     4741 ctgttgctcg cttgccgtgt cgagatcgac gccgagcacc tcgatcttgc cgtgccactt
     4801 gggcagaagg ccgatgatgg tgcgcaacag caccgacttg ccgccgcctg acttcccaac
     4861 cacgccgagg atctccccct tgcgcacgtc gagcgacaga tggtcgagca cggtctggtc
     4921 gccgaagccg acgacgaggt ccttgatcga gatgaccggc ttatccttca cgttgcctcc
     4981 gtcacatccc gatcgaagca aagaagatgg cgaacaggcc gtcgagcacg atcacgagga
     5041 agatcgactt caccaccgac gccgtggtct gcaatccgag cgactcggcg ctgcccttga
     5101 ccaacagccc ttcggcgcag gccaccaccc cgatcaccag tcccataaac ggggccttga
     5161 ccatgccaac cttgaaatgg gtgagcgaga tcgcgtcttc gagccgcgaa atatagatct
     5221 ccggactcat gccgccatag agccaagcaa cgacaccgcc gccgtaaagc gctgccatcg
     5281 atcctaggaa ggtgagcacc gggagggcga tgatcagcac gagcacgcgg ggcaggatca
     5341 gcacctcgac gggatcgaag cccatggtgc gcaaggcatc gacttcctcc cgcatcatca
     5401 tcgagccgag ctcggcggta taggagctgc cggagcggcc ggcgaccatg atggcgacaa
     5461 tcagcacgcc gagctcacgc aggacgagga tgccgaccat gtcgacagtg taggattccg
     5521 cgccgaaccg gcggaaatgg aagaacccct gctgggcgat gatcgcacct atcagaaagg
     5581 tgatcagcac gacgatggga atcgcctgca gtccgatccg gttgagctgg tagaccagcg
     5641 atgtcagccg caacgagcgc ggccggcgca cgacaccgat cacggccatg aacaaggcac
     5701 ccaacatttg cagaaagatc gagatgtcct cgcgggcctc gatcgtggcc ttgccgaggt
     5761 cgttcaactt gagcaggacg gggttgggca cggctaccgg cgccggcgta tggcggttga
     5821 cctgccgcac ctcctcca
//
LOCUS       DATNAE010000057         6021 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2166644, whole genome shotgun sequence.
ACCESSION   DATNAE010000057 DATNAE010000000
VERSION     DATNAE010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6021
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2166644"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..4833)
                     /gene="gltB"
                     /locus_tag="VN518_01145"
     CDS             complement(115..4833)
                     /gene="gltB"
                     /locus_tag="VN518_01145"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HXE03209.1"
                     /translation="MSVNHQGSNRKFTGQALPPPAQGLYDPKREHDACGVGFVADLKA
                     RPSHKIIEQGLQILENLTHRGAVGADPLAGDGAGLLVQIPHEFLKVVTAPLRISLPEP
                     GHYAVGHMFMPGNLAQRIYCQSVVERVVEAEGLQLLGWRDVPTDNSCLGSCVIETEPH
                     HRQVFIGRGPSIKDEATFERRLYILLKVISNTINGDTGGRDIGFYTVSLSCRTLVYKG
                     MFLAYQLGAYYLDLHHPKFASALALVHQRFSTNTFPSWKLAHPYRMVAHNGEINTLRG
                     NLNWMAARQASVSSPLFGKDIAKLWPISYEGQSDTACFDNALEFLVQGGYSLAHAAMM
                     LIPEAWAGNPLMDGKRRAFYEYHACLMEPWDGPAAMAFTDGRQIGATLDRNGLRPARY
                     FVTDEGLVVMASETGVLPIPEKSIVQKWRLQPGKMLLIDLAKGRIVSDEEIKSELASA
                     HPYQQWLDRTQIRVHELPGSSSPQPRTNVSLLDRQQAFGYTQESTKFLMAPMAGSGQE
                     AVGSMGNDTPVSVLSDRPKLLHTYFKQNFAQVTNPPIDPIREELVMSLVTFIGPRPNL
                     LDLEGTSKQKRLEAAHPILTNENLERIRGIGDVADNQFRTVTLDITYGADRGAAGMGE
                     ALDRLCKRAEAAVRAGENIIILSDRATGPDRIPIPSLLATSAVHHHLIRCGLRTSVGL
                     VVETGEAHEVHQFATLAGYGAEAINPYLAFETLEAMLPEIDEEITTEEAIKRYIKAVD
                     KGLLKVMSKMGISTYQSYCGAQIFDAVGLRSDFVAKYFTGTNSQVEGVGLEEIARETV
                     ERHRLAFSDAPVLRDALEVGGEYAFRIRGEAHMWRPSVVADLQHAVRGALPEKYRSYA
                     QQINEQSEQLLTLRGMFRIKTAEEMKRKPVPLDEVEPAKEIVKRFSTGAMSFGSISRE
                     AHTTLAIAMNRIGGKSNTGEGGEESDRYKPLPNGDSMRSKIKQVASGRFGVTTEYLMS
                     ADMMQIKIAQGAKPGEGGQLPGHKVDATIAKVRHSTPGVGLISPPPHHDIYSIEDLAQ
                     LIYDLKNVNPAADVSVKLVSEVGVGTVAAGVAKARSDHVTIAGFEGGTGASPLTSIKH
                     AGSPWEIGLAETQQTLVLNRLRGRISVQVDGGIRTGRDVVVGALLGADEFGVATAALI
                     AAGCIYMRKCHLNTCPVGVATQDPVLRARFQGKPEHVVNFFFFVAEEVREYMAALGFR
                     SFAEMIGQTEILDKSEAIDHWKAKGLDFTRLFHKPEVPKEVAIYNSERQDHHLDKVLD
                     RTLIELSAASIEEKKPVKIELPIRNTDRTTGAMLSGAIAKSHGHVGLPEDTIWVSLKG
                     SAGQSFGAWLANGVTLDLEGEANDYVGKGLSGGRIIVRPPADSGIVPEESIIVGNTVL
                     YGAIAGECYFRGIGGERFAVRNSGATAVVEGTGDHGCEYMTGGVVVVIGPTGRNFAAG
                     MSGGIAYVLDEEGDFERRCNLAMVELEPVPSENNVMERSRHQAGDLESHGLVDVTDMT
                     RFDEERLYQLIERHLHYTGSERARAILADWSAYLPKFVKVMPVEYRRALEEMAKAQVA
                     DKAGLGEIEIGLRGNGGNGK"
     gene            complement(5198..5659)
                     /locus_tag="VN518_01150"
     CDS             complement(5198..5659)
                     /locus_tag="VN518_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HXE03210.1"
                     /translation="MRHFDLTPLYRSTIGFDRLGSLLDTLGAFEGDTPSYPPYNIERV
                     GENEYRISMAVAGFGEKDLTIEVKENTLSIRGEKQAEVEQSTYLHRGIAGRSFERRFQ
                     LADHVVVKGANLENGLLHVDLVRELPEAMKPRTIPIETKSDKPRVIDSKAA"
BASE COUNT         1047 a         1850 c         1993 g         1131 t
ORIGIN      
        1 aattcctcaa ataaccaatg tcaccgtccc gaacggcacg tcaagcgggc ctcctcgcgc
       61 cacgctgcgg gcctgggtta tgctccagga atcttggctc cttggcgttg aggattactt
      121 gccgttgccg ccgttgccgc gcagcccgat ctcgatctcg cccaagccgg ccttgtcggc
      181 cacctgggcc ttggccatct cctccagggc tcggcggtat tcgaccggca tcaccttgac
      241 gaatttcggc aaataagcgc tccaatcggc gaggatggcg cgcgcccgct ccgagcccgt
      301 gtagtgcagg tggcgctcga tcagctgata gagacgctcc tcgtcgaaac gcgtcatgtc
      361 ggtcacgtcg acaagcccgt ggctttcgag atcccctgcc tgatggcggc tccgctccat
      421 gacgttgttc tcgctcggca ccggctcaag ctcgaccatg gcgagattgc agcggcgctc
      481 gaagtcgccc tcctcgtcga gcacataggc gatgccccct gacatgccgg cagcgaaatt
      541 ccgcccagtc gggccgatca ccacaaccac gccaccggtc atatattcgc agccatggtc
      601 gccggtgccc tcgaccacgg cggtggcgcc ggaattgcgc acggcaaagc gctcgccgcc
      661 gatgccgcgg aaatagcact cgccggcgat cgcgccgtag agcacggtat tgccgacgat
      721 gatcgactcc tcgggcacga tgccggaatc cgcgggcggc cggacgatga tgcggccgcc
      781 ggacaggccc ttgccgacat agtcgttggc ctcgccctcg aggtcgagcg tcacgccatt
      841 ggcgagccag gcgccgaagc tctggccggc agagcccttg aggctcaccc agatggtgtc
      901 ctcgggcagg cccacatggc catgactctt ggcaatggcg cccgacagca tggcaccggt
      961 ggtgcggtcg gtgttcctga tcggcagctc gatcttgacc ggcttcttct cctcgatgga
     1021 ggcggcggaa agctcgatca gcgtccggtc gagcaccttg tcgaggtgat ggtcttggcg
     1081 ctcggaattg tagatcgcca cttccttcgg cacctcgggc ttgtggaaca ggcgggtgaa
     1141 gtcgagccct ttagccttcc aatggtcgat ggcctcgctc ttatcgagga tttcggtctg
     1201 gccgatcatc tcggcgaagc tgcggaagcc gagcgccgcc atgtattcgc gcacttcctc
     1261 ggcgacgaag aagaagaagt tgaccacgtg ctccggcttg ccctggaagc gcgcgcgcaa
     1321 caccgggtcc tgggtggcga cgccgaccgg gcaggtgttg agatggcatt tgcgcatata
     1381 aatgcagccc gcggcgatca gggcggcggt cgcgacaccg aactcatcgg cgccgagcag
     1441 ggcgccgacc acgacgtcgc gaccggtgcg gatgccgcca tcgacctgca ccgagatgcg
     1501 gccgcgcaag cggttgagca cgagggtctg ctgcgtctcg gcaaggccga tctcccaggg
     1561 ggagccggca tgcttgatcg aggtgagcgg ggaggcgccc gtgccgcctt cgaagccagc
     1621 gatggtgacg tggtcagagc gcgccttggc gacgcccgcg gcgaccgtgc cgacgccaac
     1681 ctcgctcacc agcttgacgc tgacgtcggc ggcgggattg acgttcttca ggtcgtagat
     1741 gagctgtgcc agatcctcga tcgaatagat gtcgtgatgc ggcggcggcg aaatcaggcc
     1801 gacgccgggc gtcgagtggc gcaccttggc gatggtggcg tcgaccttat ggccggggag
     1861 ctgaccgcct tcgccgggct tggcgccttg ggcgatcttg atctgcatca tgtcggcgct
     1921 catcagatat tcggtggtca cgccgaagcg gcctgaggcc acctgcttga tcttcgagcg
     1981 catcgagtcg ccgttgggca gtggcttgta gcggtccgac tcctcgccgc cttcgccggt
     2041 gttcgacttg ccgccgattc ggttcatggc gatggcgagc gtggtatgcg cttcgcggct
     2101 gatcgagccg aacgacatgg cgccggtcga gaagcgcttg acgatctcct tggcgggctc
     2161 gacctcgtcg agcggcaccg gcttccgctt catctcttcg gcggtcttga tgcgaaacat
     2221 gccgcggagc gtcagaagct gctccgactg ctcgttgatc tgttgggcgt aggaccggta
     2281 tttctccggc agcgcgccgc gcacggcatg ctgcaggtcg gcgaccacgc tcgggcgcca
     2341 catatgcgcc tcgccgcgaa tgcggaaggc gtattcgccg ccgacttcga gcgcatcgcg
     2401 caacaccgga gcgtcggaga aagcgagcct gtggcgctcg accgtctcgc gcgcgatttc
     2461 ctcaagcccc acgccttcaa cctggctgtt ggtgccggtg aaatatttgg cgacgaagtc
     2521 ggagcgcagt cccacggcgt cgaatatctg ggcgccgcaa taggactgat aggtggagat
     2581 gcccatcttg gacatcactt tgagcaggcc cttgtcgacc gccttgatgt aacgcttgat
     2641 cgcctcttcc gtggtgatct cctcgtcgat ctcgggcagc atggcctcga gcgtctcaaa
     2701 ggcgaggtag gggttgatcg cctcggcgcc gtagccggcg agcgtcgcga actgatgcac
     2761 ctcatgcgcc tcgccggtct cgaccacgag accaaccgag gtccgcaagc cacagcggat
     2821 cagatgatga tgcacggcgc tggtcgcgag cagcgaggga atcgggatgc ggtctgggcc
     2881 agtcgcccgg tccgaaagga tgatgatgtt ctcgcccgcg cgcacggcgg cttcggcgcg
     2941 cttgcaaagc ctgtcgagcg cctcgcccat gccggcggcg ccgcggtccg cgccataggt
     3001 gatgtcgagc gtgacggtgc ggaactgatt gtcggcgacg tcgccgatgc cgcggatgcg
     3061 ctccaggttc tcattggtga ggatcggatg ggcagcctcg agccgcttct gcttcgaggt
     3121 gccttcgagg tcgagcaggt tcggcctcgg cccgatgaag gtcacgagcg acatgaccag
     3181 ctcctcgcgg atcgggtcga tcggcgggtt ggtgacttgc gcgaagttct gcttgaaata
     3241 ggtgtgcagc agcttcggcc ggtcggacag caccgacacc ggcgtgtcgt tgcccatgga
     3301 gccaacggcc tcctggccag atccggccat cggtgccatc aggaacttgg tgctctcctg
     3361 cgtgtagccg aacgcctgct ggcgatcgag caggctcaca ttggtgcgcg gctgcggcga
     3421 ggacgagccc ggcagctcat gcaccctgat ctgagtgcgg tcgagccatt gctgataggg
     3481 atgcgcgctc gcgagctcag acttgatctc ctcgtcggag acgatgcggc ctttggcgag
     3541 atcgatgagc agcatcttgc ccggctggag ccgccatttc tgcacgatgc tcttctcggg
     3601 gatgggcagc acgccggtct ccgaggccat gactaccagg ccttcgtcgg tcacgaaata
     3661 gcgggctggg cgcaagccgt tgcggtcgag cgtagcgccg atctgccgcc cgtcggtgaa
     3721 ggccatggcg gcagggccgt cccagggctc catcaggcag gcgtgatatt cgtagaaggc
     3781 gcggcgcttc ccatccatca gcggattgcc ggcccaggcc tccggaatga gcatcatggc
     3841 ggcgtgggcg aggctgtagc cgccttgcac caggaactcg agggcgttgt cgaagcaagc
     3901 ggtgtcggac tggccctcat aggagatcgg ccagagcttg gcgatgtcct tgccgaacag
     3961 cggcgaggag acgctcgcct ggcgcgccgc catccaattg aggttgccgc ggagcgtgtt
     4021 gatctcgccg ttatgggcga ccatgcggta gggatgggcg agcttccagg acgggaaggt
     4081 gttggtcgag aagcgctggt gcacgagcgc cagcgccgaa gcgaatttcg gatggtgcag
     4141 gtcgagatag taggcgccga gctgataggc gaggaacatg cccttgtaga cgagcgtgcg
     4201 gcaggagagc gatacggtgt agaagccgat gtcgcggccg ccagtgtcgc cgttgatggt
     4261 gttggagatc accttgagca ggatgtagag cctgcgctcg aaggtcgcct cgtccttgat
     4321 gctcggcccg cggccgatga acacctgcct gtggtgcggc tcggtctcga tcacgcaact
     4381 gccgaggcag gaattgtcgg tcggcacgtc gcgccagccg aggagctgca agccctctgc
     4441 ctcgaccacg cgttccacca ccgactggca gtagatgcgc tgtgccaggt tccccggcat
     4501 gaacatatgg ccgacggcgt agtggccggg ttccgggagc gagatgcgga gcggggcagt
     4561 gaccactttg aggaactcat gcggaatctg cacgaggagg ccggcgccgt cgccggcgag
     4621 cgggtcggcg ccgacggcgc cgcgatgggt caaattctcc agaatctgga ggccttgctc
     4681 gatgatttta tggctcggcc gggccttcag gtcggcgacg aagccgacgc cgcaggcatc
     4741 gtgttctctc tttggatcgt agagtccttg cgccggcggc ggcagagctt gccccgtgaa
     4801 cttgcgattt gaaccttgat gattgactga catcgcacgc aaacccgctt aagcgcagtt
     4861 tgtctccaaa agagcatagg ccaattcagg ctaggaacaa cgcgcaagcc cgccttcgcg
     4921 aacctagcct cgtctcaaaa aacatcgcgt taaggacggg aagggactcc gggacccgcg
     4981 cgccagcctg gagcttccgg ctgatgctcc agccacaaag gtcagcaagc ctgtcctatt
     5041 cgtcctcaaa atgacagatt tccaatggcc gcacaagcga gattaagctt gccgggcgtc
     5101 gcacaggttc cggcaagcct ccgccacggt atgaaaaagg gcgccctcga ttttgcgaag
     5161 gcgccctttc ggcaatcgat ggggtgagcg aaagcgctta ggcggccttg ctgtcgatga
     5221 ccctgggctt gtcgctcttg gtctcgatcg gaatggtgcg gggcttcatc gcctcgggca
     5281 gctcgcgcac gaggtcgaca tgcagcaggc cattctcgag gttcgcgccc ttcaccacga
     5341 cgtggtcggc aagctggaac ctgcgctcga agctgcggcc ggcgataccg cgatgcagat
     5401 aggtgctctg ctcgacctca gcctgcttct cgccacgaat cgagagcgtg ttttccttca
     5461 cctcgatggt gaggtccttc tcgccgaaac cggcgaccgc catgctgatg cgatactcgt
     5521 tctcgccgac gcgctcgatg ttgtacggcg ggtagctcgg agtgtcgccc tcgaaggcgc
     5581 caagggtgtc gagcaacgag ccgaggcggt cgaagccgat ggttgagcga taaagtgggg
     5641 taagatcaaa gtgcctcatg tcacatcctc cttgagaagc gatatgttgg gagcccgctc
     5701 gatttcgagc cgggcgtgtc gaactcgcgc aagccttgag gccttccgcg tacgaagata
     5761 tgggaatcga tgttcacacc gtcaagagtg cgtaatcgca tgattcacaa caagaaaaca
     5821 gatttgccaa gaggctgaaa aaaggggaat ttcagcgtca tggacctggt cgcaactccg
     5881 aagaacccaa tcccgctcgg cgtctccgtc ggctatctga agggcaaggg caatgtgcgc
     5941 ttgcgctacg cgcgctggcc gtcggcgctg aaagaacggc acggcacggt ctgcatcttt
     6001 cccggccggg ccgagttcat c
//
LOCUS       DATNAE010000058         2107 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2169266, whole genome shotgun sequence.
ACCESSION   DATNAE010000058 DATNAE010000000
VERSION     DATNAE010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2169266"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..901
                     /locus_tag="VN518_01155"
     CDS             68..901
                     /locus_tag="VN518_01155"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HXE03211.1"
                     /translation="MKRACVIGWPVEHSRSPIIHRYWLKLHRIDGAYAKEAVPPEEAR
                     GFLRSLGEHGYVGANVTLPHKIVALEAADVADDAARAMGAANTLWLDAEGKLNASNTD
                     AYGFMTNLAEQAPKWNEGRRPVVVLGAGGAARAVLYGLIEAGATEVLLANRSRVRAED
                     LALAFPRRVEAIAWEARARALAGCGLLVNTTSLGMRGQPPLELDLGALPPDAVVADIV
                     YSPLETPLLAEARKRGNIAVDGLGMLLHQAVPGFERWFGVRPEVTAQLKAHVAASLGG
                     A"
     gene            901..1524
                     /gene="coaE"
                     /locus_tag="VN518_01160"
     CDS             901..1524
                     /gene="coaE"
                     /locus_tag="VN518_01160"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008935030.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HXE03212.1"
                     /translation="MLVIGLTGGIGMGKSAAAAHFARSGIPVFNADSCVHRLYEGEAV
                     PAIEAAFPGVTRHGKVDRALLAAQLTGKPERLKELERIVHPMVVRREIEFLQEQEKKG
                     AWLAVLEIPLLFETGADKRVDVTVVLSAPHHVQRERVLARPGMTVERLEHLLARQLPD
                     EEKRARADFVVDSGTGLADMHREIDTLIESLRGRPGRVMERLRHQHS"
     gene            1548..>2107
                     /gene="dnaQ"
                     /locus_tag="VN518_01165"
     CDS             1548..>2107
                     /gene="dnaQ"
                     /locus_tag="VN518_01165"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HXE03213.1"
                     /translation="MREIVLDTETTGLDPFDGHRIVEIGCVELVNCIPTGRIWHYHVN
                     PEREMPYAAFEVHGLSAEFLRDKPRFAELADDMLAFIDGAMLVMHNAAFDFGFLNAEL
                     DRSARPLLRWDCVVDTLALARRRHPGAPCSLDALCKRYGIDLSERDKHGALLDCRLLA
                     RVYAELVGGHQAGLEFAVNGGQVALL"
BASE COUNT          342 a          682 c          705 g          378 t
ORIGIN      
        1 attcttgggc tgccacttat cccgctcctc gacacgcttc ggcgcgaggg cgtcatcgag
       61 gcttaaggtg aaacgcgcct gcgtcatcgg ctggccggtt gagcattcgc gctcaccgat
      121 aatccatcgc tactggctca agctccatag gatcgacggc gcctatgcga aggaggccgt
      181 gccgcccgag gaggcgaggg gattcctgcg ctcgctcggc gagcacggct atgtcggcgc
      241 caatgtcacg ttgccgcata agatcgtagc ccttgaggcc gccgacgtgg ctgacgacgc
      301 cgcgcgcgcg atgggcgcgg ccaacacgct ttggctcgac gccgagggta agctgaacgc
      361 gagcaatacc gacgcttacg gcttcatgac caatctcgct gagcaggcgc ccaagtggaa
      421 cgaagggagg cggccggtcg tggtgctcgg cgccggcggc gccgcccgcg ccgtgctcta
      481 tggtctgatc gaggccggcg cgaccgaggt gctgctcgcc aatcgcagcc gcgtgcgcgc
      541 cgaagatctc gctctcgcct ttccgcggcg cgtcgaggcg atcgcttggg aggcccgcgc
      601 ccgcgcgctt gctggctgcg gactgctcgt caacacgaca agcctcggca tgaggggtca
      661 accaccgctt gagcttgatc ttggcgcgct gcctcccgac gcggtcgtcg ccgatattgt
      721 ctatagcccg ctcgagacgc cgctattggc cgaagcgcgc aaacgcggga acatcgccgt
      781 cgacgggctc ggcatgctgc tccatcaggc ggtgccgggc ttcgagcgct ggttcggcgt
      841 cagacccgaa gtcacagcgc aactcaaggc gcatgtcgcc gcatcccttg gaggggcgtg
      901 atgctcgtca tcggcctcac cggcgggatc ggcatgggaa aatcggcggc ggcggcgcat
      961 ttcgcgcgat ccggcatccc cgtgttcaac gccgattctt gcgtgcatcg gctctatgag
     1021 ggggaggcgg ttccggccat cgaagcggcg ttccccggcg tcacgcggca cggcaaggtt
     1081 gaccgggcac ttcttgccgc gcaattgacg ggcaagcccg agcgtctcaa ggaactcgaa
     1141 cgcatcgttc atcccatggt cgtcaggcgt gagatcgagt tcctccaaga gcaggagaag
     1201 aagggcgcgt ggctggccgt gctcgagatc ccgctgctgt tcgagaccgg cgcggacaag
     1261 cgcgtcgacg tcacggtcgt gctcagcgcg ccgcatcatg tgcagcgcga gcgcgtgctg
     1321 gcgagacccg gcatgacggt ggagaggctc gaacatctgc ttgcccgcca gctgccggat
     1381 gaagaaaagc gcgcgcgggc cgacttcgtt gtggacagcg ggaccggact tgcggacatg
     1441 catcgtgaga tcgatacact tatcgaatcg cttcgaggcc gaccgggccg cgtcatggag
     1501 cggctgcgtc accaacattc ctgactaaag cgaggcggag cgaacctttg cgcgagatcg
     1561 tgctcgatac cgaaacgacc gggctcgatc ccttcgacgg ccatcgcatc gtcgagatcg
     1621 gctgcgtcga gctggtcaac tgcattccca cggggcggat ctggcactac cacgtcaatc
     1681 cggaacggga gatgccctat gccgccttcg aggtgcatgg gctctcggcc gagtttctgc
     1741 gcgacaagcc gcgcttcgcc gagctcgccg atgacatgct cgccttcatc gacggcgcta
     1801 tgctggtcat gcataacgcc gctttcgatt tcggttttct caatgccgag ctcgaccgga
     1861 gcgcgcgtcc cttgctgcgg tgggactgcg tggtcgatac gctggcgctt gcccggcggc
     1921 gtcaccccgg cgcaccgtgc agccttgatg cgctttgcaa gcgctacggc atcgacctct
     1981 ccgagcgcga caagcatggc gcgctgctcg attgccggct gctggcgcgc gtctatgccg
     2041 agctcgttgg cggtcaccag gcggggctcg agtttgccgt gaatggcggc caggtcgcct
     2101 tactgca
//
LOCUS       DATNAE010000059         5004 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3073840, whole genome shotgun sequence.
ACCESSION   DATNAE010000059 DATNAE010000000
VERSION     DATNAE010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5004
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3073840"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="VN518_01170"
     CDS             complement(<1..747)
                     /locus_tag="VN518_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXE03214.1"
                     /translation="MSASNIPTLKLPGGKIVPRLGQGTWRMGQSARRRAEEVAALKLG
                     LELGMTLIDTAEMYGDGEAERIVAEAIEGRRDEVFIVSKVLPQNASRAGTIAVCERSL
                     KRLNTDRLDLYLLHWRGRYPLRETLAGFQALLRDGLIKAWGVSNFDVDDMEELAALPG
                     GDAVATNQVLYNLARRGIESDLIPWARARGVSIMAYSPVEQGRILRDRALARVAARHR
                     AAPAQVALAWLLRHEDMMVIPKATNAAHVRE"
     gene            complement(859..1509)
                     /locus_tag="VN518_01175"
     CDS             complement(859..1509)
                     /locus_tag="VN518_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010683645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HXE03215.1"
                     /translation="MATTASGKNILEYDPARCDALSEVLAERWWVVGLRGIFGIIFGL
                     ICLLVPAAAILGLVLLFSAYMLVDGGLAIYSAVKAAQNGDRWGLLILEGIVDIAAGVV
                     AFLWPGITAVAFVLLIAVWALVSGALMLVAAFRLNIDHGRWWLALGGIASIIFGVVLI
                     IAPVVGAVVLTWWLGAYALVFGVLLLVLAFQLHSRREEKRRKPAAATKPAAAAKKA"
     gene            1943..3331
                     /locus_tag="VN518_01180"
     CDS             1943..3331
                     /locus_tag="VN518_01180"
                     /inference="COORDINATES: protein motif:HMM:NF024846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HXE03216.1"
                     /translation="MRKAPLTVCVVGGGFTGAAASIACLARIDRPFRLIVVEPSASLG
                     RGVAYGAHHPLHLLNVRTRDLSVRAGQPGDFLNWAFRQLDQGENHAGLHEGLAHTFLP
                     RQLFGEYVRERFFEAVEVRGDAELKIVNAVAISCVREGNRFTLRFDRAEKLQADVVLL
                     ATAYGLQASAPTGALAPFDALPPERISGAKTMTLIGSGLTMVDVLLGARRDGFQGKVT
                     VISKRGQLPRPHASKGVVPRESGLSRSKRLSSLTTAVAIGCAAAEAHGTPWQAVINSV
                     RSSLQDIWQGLPVEEQARFLRHVRPLWDAHRHRLPLEVHGRLQAEFNEGRAVLLRGRV
                     TKTERDGECFRLVLRRRGSKETEKLAADLAFDCTGHRPDLSSPLIQSLVAQGIARADA
                     HRLGLAVEPNGQVLSKSGAPTRGLFALGPLGQGSLWEITAVPEIVRQCDVAAQSLAAL
                     EPSAEREVELSS"
     gene            3516..4709
                     /locus_tag="VN518_01185"
     CDS             3516..4709
                     /locus_tag="VN518_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03217.1"
                     /translation="MALAAFACALGLSLSCAAQSETTQAELPQTSSVAKQSRPAAPQL
                     LAPPEESLPAANAPVITHASPPQPIPAPVPVLVSPEPEVTQPQRSEGSFSTPERLVDW
                     VYNYHAHPTPWRVPAAVRAMRELGLFTDEEKGAFCTGFIAGVLGANAKDGPALVGKML
                     PMPDKDQAVLIRAIAYSGRPDWRELLTKYKDRMPLRQPLIDSYLSGKAKPLMEIELSD
                     GPSVVYTLWGYYVATGQYEPVVRIMQGLRWSKNKNDGNFSLKKIFSNWRNDPSAVDKI
                     STGATAKWTLASYAERNRDLLSLYRAEYEHQPKEIAQPLRDVIVAAEAFESEKIRKDQ
                     FGAIEDAQKLQATNEAGMSKAATAGSIAIATGCVAASALGQIYIAVPCVIGGALYTGA
                     MKLMH"
BASE COUNT          879 a         1624 c         1612 g          889 t
ORIGIN      
        1 ctcgcgcaca tgtgcggcat tcgtggcctt ggggatcacc atcatgtcct cgtgcctgag
       61 cagccaggcg agcgccactt gcgccggcgc cgccctgtgg cgcgcggcca cgcgggcaag
      121 cgcgcggtcg cgcaagatgc ggccctgctc gactggcgaa taggccatga tggaaacgcc
      181 acgcgcgcgg gcccacggga tgaggtcgga ctcgatgccg cgccgcgcaa gattgtagag
      241 cacctggttg gtggcgacgg cgtcgccgcc gggaagcgcg gcaagctcct ccatgtcgtc
      301 aacgtcgaaa ttgctcacgc cccacgcctt gatcaggccg tcgcgcagaa gggcctgaaa
      361 tccagccagc gtctctctca acgggtatct cccgcgccag tgcaggagat agagatcgag
      421 gcggtcggtg ttgagccgct tcaggctccg ctcgcagacg gcgatggtgc cggcgcgcga
      481 ggcattctgc ggcagcacct tgctcacgat gaacacctcg tcgcggcgtc cctcgatggc
      541 ctcggcgacg atcctctcgg cctccccgtc gccatacatc tcggcggtgt cgatcagcgt
      601 catgccgagc tcgaggccga gcttgagcgc ggcgacctcc tcggcgcggc ggcgcgcgct
      661 ctggcccatg cgccaggtcc cctggccgag gcgaggcacg atcttgcctc cagggagctt
      721 gagcgttggg atgtttgacg ccgacatggg aagcctaacg tcgcggcgcg cgcattcgtt
      781 caggtgaggt cagccgctcg atgcgcttgg caagcgaggg aggggagcgc cctttgagag
      841 ccgcccctcc ttcctgcctc aggccttctt tgctgcggcg gccggtttgg tcgcagcggc
      901 gggtttacgc ctcttctctt cgcgccttga gtggagctgg aaagcgagga cgagcagcag
      961 cacgccgaac accagcgcgt aagcgcctag ccaccaggtc agcaccacgg caccgaccac
     1021 cggcgcgatg atcagcacca cgccgaagat gatcgaggcg atgccgccta gcgcaagcca
     1081 ccagcgcccg tggtcgatgt tgagccggaa ggcagcgacc agcatgagtg cgccggagac
     1141 gagcgcccag acggcgatca agaggacgaa ggcgaccgcg gtgatgccag gccagagaaa
     1201 ggcgacgacg cctgcggcga tatcgacaat gccttcaagg atgagcagtc cccagcgatc
     1261 gccgttctgg gcggccttga cggcggaata gatggcgagg ccgccgtcga ccagcatata
     1321 ggccgagaac aggagcacga gcccgaggat cgcggcggct ggcaccaaca ggcagatcaa
     1381 gccgaagatg atgccgaaga tgcctctcaa gcccaccacc caccaacgct cggcgagaac
     1441 ctcgctcaac gcgtcgcagc gcgctgggtc gtattcgaga atattcttcc cggatgctgt
     1501 ggttgccatg cttgcctcct ttggagaacc gtttgctagg tccgtcgatc gtgcgaaacg
     1561 aacagttgtt acgcgcgact cggttcggcg ccataatgcc gccaatcaaa cgcgccaagg
     1621 cgctcttttt tttcaatttc cacatcaggg aggccgcccg caatcgccat tcttggcgcc
     1681 gttcgagggc taggctcaag acctgatgtg cgtcatcgct tcgtcgtcga tcccgcgagc
     1741 ctgaaataaa tctgccggta gcgccggtgc ggggaggagg gaaatcttct cccgcggatc
     1801 ctgcatgccg cgaaatgaaa ttctgcgcaa cgcccaaatg acacgaggtc gcgcgacacc
     1861 ttcgcgagct tcgagtcatg ccctcgtctt tattccggag tttgatttgg cttaagagcg
     1921 agtgaaactt gaagggggcg agatgcggaa ggcgccactt acggtgtgcg tggtcggcgg
     1981 cgggttcacc ggcgcggcgg cttccatcgc ttgcctcgcc cggatcgacc ggccgttccg
     2041 cctcatcgta gtcgagccga gcgcctcgct cgggcgcggc gtcgcctatg gcgcgcatca
     2101 tcccttgcat cttctcaatg tgcgcacccg cgacctctct gtacgcgccg gccagcccgg
     2161 cgactttctc aactgggcct ttcgccagct cgatcagggc gagaatcacg ccggcctgca
     2221 tgagggcctc gcccacactt tcctgccgcg tcagctcttc ggcgaatatg tgcgcgagcg
     2281 cttcttcgag gcggtggagg tgaggggcga tgccgagctc aaaatcgtca atgccgtcgc
     2341 catctcctgc gtcagggagg gcaaccgttt cacccttcgc ttcgatcggg ccgagaagct
     2401 tcaagctgat gtcgtgttgc tcgccacggc ttacgggctt caggcatcgg ccccgaccgg
     2461 cgcgcttgcg cccttcgacg ctctcccccc ggagcggatc agcggcgcga agaccatgac
     2521 gctcatcggc tccggcctca ccatggtcga tgtgctgctc ggcgcgcgcc gcgatgggtt
     2581 ccagggcaag gtcacggtga tctccaagcg cgggcaattg ccgcggccgc atgcctcgaa
     2641 aggcgtggtg ccgcgcgagt ccgggctttc ccgctcgaag cgactgtcga gcctgaccac
     2701 ggcagtcgcc atcggctgcg ccgcggcgga ggcgcacggc acgccgtggc aggcggtcat
     2761 caatagcgtt cgctcctcgc ttcaggacat ctggcaaggc ttgcccgtcg aggagcaagc
     2821 gcgcttcctg cgccatgtgc gtccgctgtg ggatgcgcat cgccatcgtc tgccgctcga
     2881 agtgcatggg cgccttcaag ccgagttcaa cgaggggcgc gcggtgctgc tgcgcggccg
     2941 ggtcacgaag accgagcgcg acggcgaatg cttcaggctc gtcttgcgcc ggcgtggctc
     3001 gaaagaaacg gagaagctcg cagcagacct tgccttcgac tgcaccggtc accggcccga
     3061 tctcagctct cctttgatcc aaagcctggt tgctcagggt atagcgcgcg ctgacgctca
     3121 tcgcctcggg cttgccgtgg agccgaacgg ccaggtcttg agcaagagcg gggcgccgac
     3181 ccgaggcctg tttgctctcg ggccgctcgg ccaagggtcg ctatgggaga tcactgcggt
     3241 gccggaaatc gtgcgccaat gcgacgtcgc cgcgcaaagc ctcgccgcgc tcgagccctc
     3301 cgccgagcgt gaggtagagc tatcctcgtg aagcccgcca accccccgga ttttggcgaa
     3361 aaagggcgaa tggttaacct ctccggccct taaaaagcca cgaaactcca ggatttttaa
     3421 ccctgctgcg ggggggcata acccgcaggc gttaacgtta gggatcgtca tcacgctggg
     3481 gccggggggc aggaagctga tgagcgatgc gtgtcgtggc tttggctgcg tttgcctgcg
     3541 ctctcggact atcactctca tgcgccgctc aatcagagac cacgcaggct gaactgccgc
     3601 aaacgagttc tgtcgcgaag cagtcgcggc cggcggcgcc gcagcttctt gcgccacctg
     3661 aggaatcgct ccccgccgcc aacgctccgg tgatcaccca cgcctcgccg ccgcagccga
     3721 tcccggcgcc ggtgccggtc ctcgtttcgc ccgagccaga ggtgacgcag ccgcagcgtt
     3781 cagaggggtc gttctccacg ccagagcgcc tggtcgactg ggtctacaat taccacgccc
     3841 atccgacgcc gtggcgcgtg ccggcggcgg tgcgcgccat gcgcgagctt ggcctgttca
     3901 ccgatgagga gaagggggcg ttctgcaccg gcttcatcgc cggggtgctc ggcgccaacg
     3961 cgaaggatgg gccggccctc gtcggcaaga tgctgccgat gcccgacaag gaccaggccg
     4021 tcttgatcag ggcgatcgcc tattccggcc gcccagattg gcgtgagctg ttgaccaagt
     4081 acaaggatcg catgccgctc cgccagccgc tgatcgattc ctacctctca ggcaaggcca
     4141 aacccttgat ggagatcgag ctcagcgacg ggccttccgt ggtctatacg ctgtggggct
     4201 attacgtcgc caccggtcag tatgagccag tggtgcgcat catgcagggc ttgcgctggt
     4261 ccaagaacaa gaatgacggc aatttctcct tgaagaagat cttctccaac tggagaaacg
     4321 atccgagcgc ggtcgacaag atctcgaccg gagccactgc caaatggact ctcgcctcct
     4381 acgccgagcg caatcgcgac ctgctcagtc tctaccgcgc cgaatatgag catcagccga
     4441 aggaaatcgc ccaacccttg cgcgacgtga tcgttgccgc cgaggctttc gaatcggaga
     4501 agatccgtaa agaccagttc ggcgccatcg aagatgcgca gaagctgcaa gccacaaatg
     4561 aagccggcat gtcgaaggcc gccaccgccg gctccatcgc catcgccacc ggctgcgtgg
     4621 cggcgagcgc gcttgggcaa atctatattg ccgtgccttg cgtcatcggc ggcgcgctct
     4681 acacgggcgc gatgaagctc atgcattagg actgggggcg ttccacgcgg acaagaggga
     4741 gtgccgcatg aaacgcaatt cggggaggtg gacggtcgtc gtttcgatca tcgggggatc
     4801 ggcaatgcta ttgtgcttgg gctcggtgct gttgaccgcc gcctgtagca cgccaacgtt
     4861 gccatccgac tttcctgcgt tccatctgac gagttcgccg gatcaggaga agccggacga
     4921 gccaggtacc aactagtctg atgcgggggc ctggatcgcc gattttccag ggctgagcgg
     4981 ccgctggcgg ccgctttgat tgct
//
LOCUS       DATNAE010000060         4272 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3074384, whole genome shotgun sequence.
ACCESSION   DATNAE010000060 DATNAE010000000
VERSION     DATNAE010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4272
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3074384"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..998)
                     /locus_tag="VN518_01190"
     CDS             complement(<1..998)
                     /locus_tag="VN518_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6513 domain-containing protein"
                     /protein_id="HXE03218.1"
                     /translation="MAESILFLTGHLARPRLEAVLEGMKASFAWKVLDIGVKVAALMT
                     EEIIARRLPKPVEADKIMLPGRCRADLDRLSERFGVPFVRGPDELKDIPGYFGRAAKA
                     SDMTKYDIQIFAEIVDASALPIEAILARAEDYVRKGANVIDLGGLPDTPFPHLEQAVK
                     ALKAKGYRVSVDSADPEELLRGGKAGADYLLSLNEDTLSIADKLKSTPVLIPNEHGDL
                     PSLYRAMDALDAKGRAYLADPVLDPINFGFMQSLERYARVRRDRPKAELLLGTGNLTE
                     LTDADTTGITAVLLGIASELHIRNVLLVQVSPHTRRTVEEHDLARRIMYASRADHDL"
     gene            complement(1090..1767)
                     /locus_tag="VN518_01195"
     CDS             complement(1090..1767)
                     /locus_tag="VN518_01195"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03219.1"
                     /translation="MAMPGQALPAMTDEHALALKQRPVVVKLGGSVVRSAELPAWLDA
                     IAASPLPIVVVPGGGALADEVRAAQARLGFGNGAAHRMALLAMDQLAWAVAGLRAGFE
                     VGDTEEALRASLARGRVAVWAPYSLVADRSDIPQSWIVTSDSLALWLGRRLGAACCYV
                     IKSMERRHAKFGAGQLARDGVVDEAFPAMLRDAGFPVFLLGRGDQRAFTAWLSDMSRP
                     PCGATID"
     gene            complement(1767..2192)
                     /gene="folB"
                     /locus_tag="VN518_01200"
     CDS             complement(1767..2192)
                     /gene="folB"
                     /locus_tag="VN518_01200"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HXE03220.1"
                     /translation="MPMTEERVTRAGRVSSSGSGTDAPYDRVFVHDLVLDAEIGVYTH
                     EKGVTQRARFSVDIEVTPTDQAIDDQIGRVLDYDTIIDTIKGILAEGHINLVETLADE
                     IASRCLAHPRAASVKVKIEKLDKEPGAVGVEIVRRRVRS"
     gene            complement(2225..2962)
                     /locus_tag="VN518_01205"
     CDS             complement(2225..2962)
                     /locus_tag="VN518_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(5-formylfuran-3-yl)methyl phosphate synthase"
                     /protein_id="HXE03221.1"
                     /translation="MTLFLASVRDEAEAAIALLARADIIDLKEPRGGALGALDRETTR
                     SIVSLLGGRARLSATIGDLPMQPETIGAAVLEKADCGVDYVKFGLFPDGDARRCLDAL
                     RPLARQVNLIVVLFADRLPDFDAVAAAAAMGAAGIMLDTANKGSGSLLAHLDPRQLAN
                     FVREAKSHGLTIGLAGSLAAADVPDLLPIASDLLGFRGALCNGSRGASLDPAACAAIR
                     ALIPVNQPAAESKLPARMSEAPAQALC"
     gene            complement(3020..4255)
                     /locus_tag="VN518_01210"
     CDS             complement(3020..4255)
                     /locus_tag="VN518_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXE03222.1"
                     /translation="MKAAPPKKPEPVSTVAKQPKAAPADPNAAPPAVPGAPADANAAA
                     PGATPSADPNAAAAPVDPNAVTAPADANAAPGTPTAAADPNAAATPEDLNAATPVDPN
                     ADSVPADSNAPAVPADPNAAAPTDTNAAAPGAMAAADPNAVVPPDAGADTNAAPAADA
                     APEPVQADDEALKDLQQALTIAPDDPVALRLRGDFYQATGKTPEAIADYQAALAKDPF
                     QSESRDALEKLGQDVPDEQGQPLAPPVSDWVVTEPSPGRYVATNPKYKKLRAELEMFG
                     SGKPKIIEWTQMKDALAGIGLLRYYAGDLAEGANQELVYTAIIDLWANKVVTIEPYSW
                     GTNAAKWDWQAYSVVVTDPDGTPNEIKLRKPKVRPVAREGGFFDPWGGPAQSQAQPRG
                     RRGGGGGGGGGGLFGWFGR"
BASE COUNT          707 a         1389 c         1467 g          709 t
ORIGIN      
        1 aggtcgtggt cggcgcggga ggcatacatg atgcgccggg caaggtcgtg ctcctcaacg
       61 gtgcgccgcg tatgcgggct cacctgcacc aagaggacat tgcggatgtg gagctccgac
      121 gcaatgccga gcagcacggc ggtgatgccg gtggtgtcgg cgtcggtgag ctcggtgagg
      181 ttgccggtgc cgagcagcaa ctcggccttg ggccggtcgc ggcgcactcg cgcgtagcgc
      241 tcgagcgact gcatgaagcc gaagttgatc ggatcgagaa ccgggtcggc aaggtaggcg
      301 cggcctttgg cgtcgagcgc gtccatggcg cggtagagcg agggaagatc gccgtgctca
      361 ttggggatca gcaccggcgt cgatttgagc ttgtcggcga tcgacagagt gtcctcgttg
      421 aggctcagca gataatcggc gcccgccttg ccgccgcgca agagttcctc gggatcggca
      481 gagtcgacgc tcactctgta gcccttcgcc ttcagcgcct tgaccgcctg ctcgagatgc
      541 gggaacggcg tgtcgggcag gccgccgaga tcgatgacgt tggcgccctt gcgcacatag
      601 tcctcggcgc gggcgaggat cgcttcgata ggcagtgccg aggcatcgac gatctcggcg
      661 aagatctgga tgtcgtattt ggtcatgtcc gaggccttgg ccgcgcggcc gaaatagccc
      721 gggatgtctt tcagctcgtc ggggccgcgc acgaagggca cgccgaagcg ctcgctcagg
      781 cggtcgaggt cggcccggca gcggccaggc agcattatct tgtcggcctc aacgggcttc
      841 ggcagccggc gcgcgatgat ctcctcggtc atcaaagccg cgaccttcac cccgatgtcg
      901 agcaccttcc aggcgaagct cgccttcatg ccctcgagca ccgcctcaag ccgcgggcgc
      961 gccaggtgtc cggtgaggaa gaggatgctt tcagccattg acggtccgct tggtgctaag
     1021 ccagtgccga cttctacccc gcctcgcagg caagcgcgag ggcgcggctc caggcttaac
     1081 gcattggcgt tagtcgatgg ttgcgccgca agggggccgg ctcatatccg aaagccatgc
     1141 cgtgaaggct cgttgatcgc cgcggccgag caggaacacc gggaagcctg cgtccctcaa
     1201 catggcgggg aaggcctcgt cgacgacacc gtcgcgggcg agctgcccgg cgccgaactt
     1261 ggcgtggcgc cgctccatgg acttgatcac gtagcagcac gcggcaccga gacgccgccc
     1321 gagccacaga gcgaggctgt ccgaggtgac gatccaggat tgagggatgt cgctgcggtc
     1381 ggcgacgagg ctgtagggcg cccacaccgc cacacggcca cgcgcaagcg aggcgcgcaa
     1441 cgcctcctcg gtgtcgccaa cctcgaagcc ggcgcgcaag cccgcaaccg cccatgcgag
     1501 ctgatccata gcgagcagcg ccatgcggtg ggccgcgccg ttgccgaagc cgagcctcgc
     1561 ttgcgccgcg cgcacctcgt cggccaaggc ccctccaccc ggcacgacga cgatcggaag
     1621 cgggcttgcc gcgatggcat cgagccaggc cggcagctcg gctgagcgca ccacactgcc
     1681 gccgagcttc accacgacag gccgttgctt gagcgcaagc gcgtgctcat cggtcatcgc
     1741 cggcagagcc tggccgggca tggccatcag ctccgcacgc gccggcgcac gatctcaacg
     1801 ccaaccgcgc ctggctcctt gtcgagtttc tcgatcttca ccttcacgga agccgcgcgc
     1861 ggatgggcaa ggcagcgcga ggcgatctcg tcggccaagg tttcgaccag gttgatatgg
     1921 ccttcggcga ggatgccctt gatggtgtcg atgatcgtgt cgtagtcgag cacgcgaccg
     1981 atctgatcat cgatcgcctg atcggtcggt gtcacttcga tgtccaccga gaagcgggcg
     2041 cgctgggtga cgcccttttc atgggtatag acgccgatct cggcatcaag caccaggtcg
     2101 tgcacgaaga cgcggtcata aggagcatcg gtgccgctgc cgctggagga cacacggccg
     2161 gctcgcgtca ctcgctcttc cgtcatcggc atcagggact ccgtcgggat tgcagccgct
     2221 ccgcttagca taacgcttga gcgggcgctt cggacatcct tgccggcagt ttgctttccg
     2281 ctgccggctg gttcaccggg atcaaggcgc ggatggcggc gcaagcggca ggatcgaggg
     2341 aggcgccgcg ggagccgtta caaagtgccc cgcggaagcc gagcaggtcc gacgcaatgg
     2401 ggagcaggtc aggcacatcg gccgcggcga gcgagccggc gaggccgatc gtcaggccat
     2461 gcgacttcgc ctccctcacg aaattcgcga gctgacgagg atcgaggtga gcgagaagcg
     2521 agcctgagcc cttattcgcg gtgtcgagca tgatcccggc agctcccatg gctgccgcgg
     2581 cggccacggc gtcgaagtcc ggcaagcggt cggcgaacag cacgacgatt aggttgacct
     2641 gccgcgccag cgggcgaagc gcatcgagac agcgccgcgc gtcaccatcg gggaacagcc
     2701 cgaatttcac gtagtcgacg ccgcagtcgg ccttctccaa caccgcagcg ccgatggtct
     2761 ccggctgcat cggcagatcg ccgatggtgg cgctcagccg agcacgcccg ccgaggagcg
     2821 acacgatgct gcgcgtggtc tcccggtcga gcgcgccaag cgcgcccccc cgaggctcct
     2881 tcagatcgat gatgtcggcg cgggcgagga gagcgatcgc agcttcggcc tcgtcgcgca
     2941 cgctggcaag gaagagggtc atggtgtcct gggtccgggg cgagacgtcg caatcacgaa
     3001 caataaggca gaggacacac tatcggccga accagccgaa aagtccgcct cctcctccgc
     3061 ccccgccgcc gccgcggcga ccgcgaggct gagcctggct ctgtgccggc ccaccccatg
     3121 ggtcaaagaa gccgccctcg cgggcaacag gtctgacttt cggcttccga agcttgatct
     3181 cattgggggt gccgtcgggg tcggtcacca ccacagaata cgcctgccaa tcccatttgg
     3241 cagcgttggt gccccagcta tagggctcga tggtgaccac cttgttcgcc cataggtcga
     3301 tgattgccgt atagacgagc tcctgattgg ccccctccgc caaatcgccg gcgtagtacc
     3361 gaagcaggcc gatgccggcc agcgcatcct tcatctgggt ccattcgatg atcttgggct
     3421 tgcccgagcc gaacatctcg agctcggcgc gcaacttttt gtactttgga ttggtcgcga
     3481 cgtaacgccc gggggacggt tcggtcacga cccagtcgga caccggaggc gccaagggct
     3541 gcccctgctc gtccggaaca tcctgaccga gcttctcgag cgcatcccgg ctctcgctct
     3601 ggaaggggtc cttggcgagc gctgcctgat agtcggcaat cgcctctggc gtcttgccgg
     3661 tcgcttgata gaagtcgccg cgaaggcgca aggcaaccgg atcgtcaggg gcaatcgtca
     3721 gcgcctgctg caggtctttc agcgcttcgt cgtcagcctg aacgggctcg ggagcagcat
     3781 ccgccgctgg cgcagcgttc gtgtcggcgc cggcgtcagg cggaaccacg gcattcggat
     3841 cggccgccgc cattgcgccg ggggccgccg cgttcgtatc ggtcggagcc gccgcattgg
     3901 ggtcggcagg tacagccggg gcgttcgaat cggccgggac gctgtcagca ttcggatcaa
     3961 caggggtagc agcatttaaa tcttcaggag tggctgccgc gttcggatca gcggcggcgg
     4021 tcggcgtacc cggagctgca ttcgcatcgg cgggagccgt cacggcattc ggatcgacag
     4081 gtgcagccgc agcgtttggg tcggcagaag gcgtcgcgcc tggggcagcc gcattcgcat
     4141 ccgcaggcgc accggggacg gcaggggggg cagcgttcgg gtcggcaggc gcggctttgg
     4201 gctgtttggc aacggtggac acgggctccg gcttcttcgg cggtgccgcc ttcatccttg
     4261 cctcggcgcg ca
//
LOCUS       DATNAE010000061         2201 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_668365, whole genome shotgun sequence.
ACCESSION   DATNAE010000061 DATNAE010000000
VERSION     DATNAE010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668365"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="VN518_01215"
     CDS             <1..843
                     /locus_tag="VN518_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03223.1"
                     /translation="LCLKEGRPLVDYLDEVALGGLKLAERDAERGSLDAENLEAIDAT
                     VDEMMDNLTDFEPRRWFRKVRTEIEVEEAPGGLASLTNLEEEEVDQLPMLEGALAPGW
                     EAQDAVLCVGGRTPLDEAAASMLAGALQRQGLKARSLPSDAISASHIVSLEASKTKLV
                     CLSYFGANANAAHVRYLVRRLRRILPQGATVLVGFWADDGGGAALKSLEATAEADAYA
                     TTLKEAARFCINAARSHEPEKSEALAKSDTAAKDDKAPKDAKAPPAKGEKPAKDEKAP
                     ARVA"
     gene            complement(865..>2201)
                     /locus_tag="VN518_01220"
     CDS             complement(865..>2201)
                     /locus_tag="VN518_01220"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HXE03224.1"
                     /translation="AEPAPAAEPAPQRSSWSSGLVVMAFALVALVPVIAFGALYSRGA
                     LSGASLPAVIGEDRTEQTAQAAQPAPQAEPVSPVAEPMPPTVQQPPQAVQQAGAATPA
                     SSEGSPASETILAKRDIELPPVTLTTPASVVGEAGKETAFAISLDSSENLPPRSIITI
                     RGLPEGTSFSAGRPFGETEWSLRPDEISGLRLKLPAVASGRRALSVNVVAADGTMIAS
                     SSTRLDIAPDPKAALILRPQDTARIDELMAHGRKMVDVGYFPGARAYFQRAAEAGSAE
                     AAFSVGLSYDPSFIAEMGAQGIKPDVKQARIWYERAKALGYKDADAKLNALSNSEAAI
                     EPAPAPAAPKPAEAPVVPVVTTPQPVVEPAATPQPSETGAADQEWVEVSSPVNMRSAP
                     TPQADTVNVAEPGKRYQATGRQGSWVQVTDPATSEVGWVYARYVAASEPPNR"
BASE COUNT          356 a          739 c          743 g          363 t
ORIGIN      
        1 ctgtgcctga aggaagggcg cccgctcgtg gactatctcg acgaggttgc tctcggcggc
       61 ctcaagctcg ccgagcgcga cgccgaacgc ggttcgctcg acgccgagaa cctggaagcc
      121 atcgacgcca ccgtcgacga gatgatggac aatctcaccg atttcgagcc acggcgctgg
      181 ttcaggaagg tgcgcaccga gatcgaggtc gaggaagccc ccggcggatt ggcgtcgctc
      241 accaatctcg aggaggagga agtcgaccag cttccgatgc tcgagggcgc gcttgctccg
      301 ggctgggagg cgcaggatgc ggtgctttgc gtcggcggac gcacgccgct ggatgaggcc
      361 gccgccagca tgctcgccgg agccctgcaa cggcagggcc tcaaggcccg ctcccttccg
      421 agcgatgcca tttccgcctc ccacatcgtc tcgctcgaag cctcaaagac gaagctcgtt
      481 tgcctctctt attttggcgc gaacgcgaac gcggcccatg tccgctatct cgtgcgccgg
      541 ttgcgccgca tcctgccgca gggcgccacc gtcctggtcg gcttttgggc tgatgacgga
      601 ggcggggcag ctctcaagtc tctggaagcg accgccgaag ccgatgccta tgccactacg
      661 ttgaaggaag ctgcgcgctt ctgtatcaat gccgcccgta gtcacgagcc ggaaaagagc
      721 gaggcactgg cgaagagcga taccgccgcc aaggacgaca aggctcccaa ggacgctaag
      781 gcgccgccgg caaagggcga gaagccggcg aaggacgaga aggctccggc acgcgtcgcc
      841 tgagttcgtc ctatcagcgg ctctttaccg gttcggcggc tctgatgccg ccacgtagcg
      901 ggcatagacc cagccgacct ccgacgtcgc agggtcagtg acctgcaccc agctgccttg
      961 ccggccagtg gcctggtagc gcttcccggg ctcggcgacg ttgaccgtgt ctgcttgcgg
     1021 cgtgggcgcc gagcgcatat tcaccgggct cgaaacctcg acccattcct gatcggcagc
     1081 acctgtctcg ctcggctgag gcgtcgctgc cggctcgacg accggctgcg gcgtggtcac
     1141 caccggcacc acgggagcct ccgccggctt cggcgcggcc ggcgctggcg ccggctcaat
     1201 tgcggcttcc gaattggata gcgcgttgag cttcgcgtcg gcatccttgt agccgagcgc
     1261 cttggcgcgc tcgtaccaga tacgggcctg cttcacgtcg ggcttgatgc cttgcgcgcc
     1321 catttcggcg ataaacgacg gatcgtagga gaggccgacc gaaaaggctg cttcggcgct
     1381 cccggcttcg gccgcacgct ggaagtaggc tcgggcaccg gggaaatatc cgacgtcgac
     1441 catcttgcgg ccatgggcca tcagctcgtc gatgcgcgcg gtgtcctgcg ggcgcaagat
     1501 gagcgcagcc ttcggatccg gcgcgatgtc gaggcgcgtg ctcgagctcg cgatcatcgt
     1561 gccatcggcc gccaccacgt tgacgctaag cgcccgccgg ccgctcgcga ccgccggaag
     1621 ctttaggcgc aagccgctga tctcgtcggg cctcaagctc cactccgtct cgccgaaggg
     1681 acgcccggcg gagaagctcg tcccctccgg caggccgcga atggtgatga tgctgcgcgg
     1741 aggcagattt tcgctgctgt cgagcgatat ggcgaaagcg gtctccttgc cagcctcgcc
     1801 gacgacggaa gccggcgtcg taagcgtcac gggtggaagc tcgatgtccc gcttggcaag
     1861 gatcgtctcg gacgcaggag acccctcgga cgaagcaggc gtcgcggcgc cggcttgttg
     1921 cacggcttgc ggcggttgtt gcaccgttgg cggcattggc tctgccacgg gagacactgg
     1981 ttccgcttgc ggcgccggct gcgcggcttg cgccgtctgc tcggtgcggt cttcgccgat
     2041 caccgcgggc aagctcgcac cgctcagcgc gccgcgcgaa taaagcgcgc caaaggcaat
     2101 gaccggcacg agcgcgacaa gcgcgaaggc catcacgacc aggccgctcg accacgacga
     2161 acgttgcggc gcaggctcgg ctgctggggc cggctccgct a
//
LOCUS       DATNAE010000062         3395 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_4284449, whole genome shotgun sequence.
ACCESSION   DATNAE010000062 DATNAE010000000
VERSION     DATNAE010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4284449"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..1199
                     /gene="aroB"
                     /locus_tag="VN518_01225"
     CDS             48..1199
                     /gene="aroB"
                     /locus_tag="VN518_01225"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HXE03225.1"
                     /translation="MRDGATALAGSQSETIIEVKLPGRSYPIVIGDTLIASAGRRIAA
                     ALPGARCAVVSDRNVAALYLAALKASLDRESLFLGEAVISPGEASKSFPVLAPLCESL
                     LELGVERGDCVIALGGGVVGDLAGFAASILRRGVRVVQIPTSLLAQVDSSVGGKTGID
                     TRQGKNLIGTFHQPSLVLADVGTLSTLSQREFRAGYAEVAKYGLIGDAPFFTWLEQNW
                     PEVFATPGDKRQHAVETSVRAKALIVAADEREESGTRALLNLGHTFGHALEAFAGYSD
                     RLLHGEAIAIGMRLAFTFSVELGLCPAGDAARVARHLEAVGLPVKISAIPRGTPKPEE
                     LLRLMAQDKKVKGGRLVLVLARGIGQAFVERDVSVPSLTQFLIQECGRR"
     gene            1196..2500
                     /locus_tag="VN518_01230"
     CDS             1196..2500
                     /locus_tag="VN518_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HXE03226.1"
                     /translation="MTLGLGITLGAILGLLLASAFFSGSETALTAASAAKMHALEEGG
                     SRRARVVNYLLTIRERFIGAILLGNTLANVAASALATSLFLTLFGEEGVVYATIVMTV
                     IVVLFAEVLPKTYAILNADRMALTVAPVVRFVVAVLAPVTATMQWIVRHTLRLFGVSI
                     SDDAEVLSAHEEIRGAIELHHKEGGVVKLTRDMLGGVLDLRELTVSDIMVHRTKMDAI
                     DAELPTAEIVDLALKSSHTRVPLWRGEREEIIGVLHAKQLLRALRDSKGDLSKLDIMA
                     VAVPPWFVPDTTTLKDQLNAFLKRKAHFAIVVDEYGEVMGLVTLEDIIEEIVGDITDE
                     TDIAAALAKPQPDGSLIVDGTVPIRDVNRLMDWDIPDEEATTIAGLVIHEAQTIPDTG
                     QSFTFHDFRFEVLRRQRNRLTSLRVTPLKPARKRKKASSKAS"
     gene            complement(2495..2776)
                     /locus_tag="VN518_01235"
     CDS             complement(2495..2776)
                     /locus_tag="VN518_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HXE03227.1"
                     /translation="MRVEEQITQKLRQAFAPVALEVVNDSHRHAGHAGSPQTGESHFS
                     IKVVSQSFAGKSRLERHRMVNEVLAEELAGKVHALAISALAPEERSHPL"
     gene            2873..>3395
                     /locus_tag="VN518_01240"
     CDS             2873..>3395
                     /locus_tag="VN518_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009466349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HXE03228.1"
                     /translation="MKLDSKLYDRLRVKPDADRLLRDSCPKCDRPGCSEPGLYPAPKG
                     RGQEGQYHRFCLDHVREYNKGYNYFAGVPDEDVVKHQKDDTIGHRPTWTIGVNPWAKK
                     RGPRNGAPAGGFAHRFSTVDPFNLFAERATVKPGPGEPERPIKRAERKCLRQLNLEDN
                     ASKADIKARFKELV"
BASE COUNT          597 a         1121 c         1099 g          578 t
ORIGIN      
        1 ggatacgagg cgaatgcggc cagcgagggc cacaagaagg tgaaatgatg cgggacggcg
       61 cgaccgcctt ggctggaagc caaagcgaga caatcatcga agtgaagctg ccgggacgga
      121 gctacccgat cgtcatcggc gatacgctga tcgcctcggc gggccggcgc atcgcggccg
      181 cgcttcccgg cgcgcgctgc gcggtggtca gcgaccgcaa cgtggcggcg ctctatctcg
      241 cagcgctgaa ggcgagcctc gatcgggaga gcctgttcct tggtgaggcg gtgatcagcc
      301 ccggcgaggc cagcaagagc ttccccgtgc tggcgccgct ctgcgaaagc ctgctcgagc
      361 tcggcgtcga gcggggcgat tgcgtcatcg cgctcggcgg cggcgtggtc ggcgatctgg
      421 ccggcttcgc cgccagcatc ctgcggcgcg gcgtccgcgt cgtgcagata cccacctcgc
      481 tgttggcgca ggtggattcc tcagtcgggg gcaagaccgg catcgacacg cggcaaggca
      541 agaatctgat cggcaccttc caccaaccga gcctggtgct cgccgacgtc ggcacgctct
      601 cgaccttgag ccagcgcgag ttccgcgcgg gctatgccga agtggcgaaa tacggcctga
      661 tcggcgatgc gccgttcttc acctggctcg aacagaattg gcccgaggtg tttgccactc
      721 ccggcgacaa gcgccagcat gcggtcgaga ccagcgtccg cgccaaggcc ctgatcgtgg
      781 cggccgacga gcgcgaggag agcggcaccc gcgctctgct caatcttggg catactttcg
      841 gccatgcgct cgaagccttc gccggctatt cggatcggct cttgcacggc gaggcgatcg
      901 ccatcggcat gcgccttgcc ttcaccttct cggtcgagct cgggctttgc ccggcaggcg
      961 acgccgcccg cgtcgcgcgg catctcgaag ccgtcgggct gccggtcaaa atctcggcaa
     1021 ttccccgcgg aacgcccaaa cccgaagagc tgttgcgcct gatggcccag gacaagaagg
     1081 tgaagggcgg caggctcgtg ctggtgctgg cgcgcggcat cggccaggcc ttcgtcgagc
     1141 gtgacgtctc ggttccgtct ctcacgcagt ttctcattca ggagtgtggc agacgatgac
     1201 tctaggcttg ggtattacgc ttggcgcaat cctgggtctc ctgctcgcct cggccttctt
     1261 ctccggctcc gagacagccc tgaccgcagc ctccgccgcc aagatgcatg cgctcgagga
     1321 gggaggcagc cgccgtgcgc gcgtggtgaa ctacctgctc accattcgcg agcgcttcat
     1381 cggcgccatc ctgctcggca acacgctggc caatgtcgcc gcatccgcgc tcgccaccag
     1441 cctgttcctg accctgtttg gcgaggaggg cgtggtctac gcgaccatcg tcatgacggt
     1501 gatcgtggtg ctgttcgccg aggtgctgcc caagacctac gcgatcctga acgccgaccg
     1561 catggcgctt accgtcgcgc cggtcgtgcg tttcgtcgtg gcggtgctgg cccccgtaac
     1621 cgccacgatg cagtggatcg tccgccacac cttgcgtctg ttcggcgttt cgatcagcga
     1681 cgacgccgag gtgctctcgg cgcacgagga gatcaggggc gccatcgagc tgcatcataa
     1741 ggaaggcggg gtggtgaagc tcacccgcga catgctgggt ggcgtgctcg atctgcgcga
     1801 gctgaccgtg tcggacatca tggtgcaccg gaccaagatg gacgccatcg acgccgagct
     1861 tcctaccgcg gagatcgtcg acctcgccct gaaaagctcg catacgcgag tgccgctatg
     1921 gcggggcgag cgcgaggaga tcatcggcgt cctgcacgcc aagcagctcc tcagggccct
     1981 gcgggactcc aagggcgacc tgtccaaact cgacatcatg gccgtcgcgg tgccgccctg
     2041 gttcgttccg gacaccacga cgctgaagga tcagctcaat gccttcctca aacggaaggc
     2101 gcatttcgct atcgtcgtcg acgagtatgg ggaggtgatg ggcctcgtca cgcttgagga
     2161 catcatcgag gagatcgtcg gcgacatcac cgacgagacc gacatcgcgg cggcgctggc
     2221 caagccgcaa cccgacggat cgctgatcgt cgacggcacc gtgccgatcc gcgacgtgaa
     2281 tcggctgatg gactgggaca tccccgacga ggaagcgacc accatcgccg gcctggtgat
     2341 tcacgaggcg cagaccatcc ccgacacggg gcagtccttc accttccacg atttccgctt
     2401 cgaggtgctg aggcggcaga ggaaccggct caccagcttg cgggtgacgc cgctcaaacc
     2461 ggcgcgcaag cgaaagaagg cctcgagcaa ggcctcatag cgggtgcgag cgctcctcgg
     2521 gcgcgagcgc gctgatggca agcgcatgga ccttgccagc aagctcctcg gccaagactt
     2581 cgttgaccat gcggtgacgc tccaaccgcg acttgccggc gaagctctgc gacaccactt
     2641 taatgctgaa atgactctcg ccggtttggg gcgagccggc gtggccggca tgtcgatggg
     2701 aatcgttgac cacttcgagt gcgaccggcg cgaaggcttg tcgcaacttt tgtgtgattt
     2761 gctcttcaac gcgcatcgcg ccgcctccac ctaaaccgcg catcttaacc taatcgatgg
     2821 cgtgagggcc attcttgttg tgacaagcgc tacttctcat aatgccgatt ccatgaagct
     2881 cgactcgaaa ctctacgacc gcttgcgcgt caagcctgac gccgaccgcc tgctgcgcga
     2941 ttcatgcccg aagtgcgacc ggccgggctg cagcgagccc ggactctacc cggcgcccaa
     3001 gggccgcggg caagaaggcc agtatcaccg cttctgcctc gatcacgtgc gcgaatacaa
     3061 caagggctac aattatttcg ccggagtgcc cgacgaggac gtcgtcaagc accagaagga
     3121 cgacacgatc ggtcaccgcc cgacctggac catcggggtg aacccgtggg cgaaaaagcg
     3181 cgggccgcgc aatggcgcgc ccgccggcgg ctttgcccat cgcttctcga ccgtggatcc
     3241 gttcaacctg ttcgccgagc gcgccaccgt caagccgggg ccgggcgagc cggagcggcc
     3301 gatcaagcgc gcggagcgga aatgcctgcg tcagctcaat ctcgaggaca acgcctccaa
     3361 ggccgacatc aaggcgcgct tcaaggagtt ggtta
//
LOCUS       DATNAE010000063         2116 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_1274556, whole genome shotgun sequence.
ACCESSION   DATNAE010000063 DATNAE010000000
VERSION     DATNAE010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1274556"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..730
                     /gene="lexA"
                     /locus_tag="VN518_01245"
     CDS             29..730
                     /gene="lexA"
                     /locus_tag="VN518_01245"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HXE03229.1"
                     /translation="MLTSKQKELLMFIHERLKETGVPPSFDEMKEALDLKSKSGIHRL
                     ITALEERGFVRRLPHRARAMEIVRLPDSMPQPLAQRSRGFSPSVIEGSLGRVPQVAEG
                     ESLPQTVSVPVMGRIAAGVPVEAIQTRLSTVEVPPELLTKGEHYALEVKGDSMIEAGI
                     LEGDTIIVRKCEDASNGDIVVALVDEEEATLKRLRRRGDSIALEAANPAYETRIFGPD
                     RVRVQGRLVGLLRRY"
     gene            complement(740..>2116)
                     /locus_tag="VN518_01250"
     CDS             complement(740..>2116)
                     /locus_tag="VN518_01250"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HXE03230.1"
                     /translation="GLHMVIMAGTVFWLVRALLALVPVLALRYPIKKWSAAAALAAAA
                     FYLALSGAAVPTVRSWIMMSIVLIAVMLDRPALTMRNVALAALAILIVAPESLFDPSF
                     EMSFAAVVALVALYEWLSARSRERLGDVSLLWGTLRKGWALISGAAVTTLVAGAAITP
                     FAIYHFHRMTHYGLIANLIAAPLVSLLIMPMAVLSLIAMPFGLDAWPLKAMGLGIELM
                     VGAGEWVASWPGAVTILPAISGSGLVLIVLGGLWVCLWQTRTRALGLVIAAFGIALAP
                     GSERPDILIEREGATAALRSDSGNLVFPPATAANYSVDNWLLADGDDRDAAAASSGEG
                     AFRCDTLGCIGTVKGKTVALIRHEGAVEEDCRIADIVIAPFTIGQRCRAARVIVDRRV
                     LKAEGAHALYIEGLSIRTETVAAWRGHRPWVPDRASVRSTPHSRLDPFADEGDDAPDK
                     SLSGNPED"
BASE COUNT          393 a          698 c          700 g          325 t
ORIGIN      
        1 acaaagtacc aaccgctcag aggggtccat gctcacgtcg aagcaaaaag agcttcttat
       61 gttcattcat gagcggttga aggagaccgg cgtacccccc tctttcgatg agatgaagga
      121 ggcgctcgac ctcaagtcga aatcgggcat ccatcgcctg atcaccgcgc ttgaggagcg
      181 cggttttgtc cgccgcctgc cccatcgggc gcgggcgatg gagatcgtcc ggttgcccga
      241 ctccatgccc cagccgcttg cccagcggtc ccgcggcttc tctccgagcg tgatcgaagg
      301 cagcctgggc cgcgtgcctc aggtcgccga gggggagagc ctgccccaga ccgtgagcgt
      361 gccggtcatg ggccgcatcg ccgcgggcgt gcccgtggag gcgatccaga cccggctcag
      421 caccgtcgag gtgccgcctg agcttctcac caaaggcgag cactacgccc tcgaggtcaa
      481 aggcgattcc atgatcgagg cgggcatcct cgaaggcgat acgatcatcg tcaggaaatg
      541 cgaggacgcg agcaacggcg atatcgtcgt ggcgcttgtc gacgaggagg aagcgacgct
      601 gaagcgcttg cggcgcagag gcgactccat cgcgctggaa gccgcaaatc ccgcctacga
      661 gacgcgcatc tttggtcccg accgggtccg ggtgcaaggt cgccttgttg gcctgctccg
      721 ccgctactag caagcccctt cagtcttctg gattgccgct gaggctcttg tcgggtgcat
      781 cgtctccctc atctgcgaag gggtccagcc gactgtgcgg cgtgctccgc acgctggccc
      841 tatcggggac ccaaggccga tgcccgcgcc aggccgcgac cgtctcggtc ctgatcgaca
      901 ggccttcaat atagagcgcg tgcgcgccct cagccttcag cacacgccga tcgacgatta
      961 cccgggctgc gcggcatctc tggccgatgg tgaagggagc gatgacgata tcggcgatgc
     1021 ggcaatcctc ctcgaccgct ccttcatggc gaatgagcgc aacggtcttg cctttgaccg
     1081 tgccgatgca gccgagggtg tcgcagcgga aggcgccctc gccggatgaa gcggcggccg
     1141 cgtcgcggtc atcgccgtcg gcgagcagcc aattgtcgac gctgtagttt gctgccgtcg
     1201 ccggcggaaa gacgagattg ccgctgtctg acctgagcgc cgccgtggcg ccttcccgct
     1261 cgatgagaat gtcaggccgc tcacttccgg gcgcgagtgc gatgccgaag gcggcgatga
     1321 cgaggccgag cgcgcgggtg cgcgtctgcc acaggcagac ccacaggccg cccagcacga
     1381 tcaggacgag gcccgagccg gagatcgcgg gaaggatggt gacggcgccg ggccaagatg
     1441 ccacccactc gccggcgccc accatcagct cgatgccgag ccccatggct ttcagcggcc
     1501 aggcatccag gccgaacggc atggcgatca agctcagtac cgccatgggc atgatcagga
     1561 ggctgacgag cggcgccgcg atcagattgg cgatcaatcc gtaatgcgtc atccgatgaa
     1621 agtggtagat ggcaaacggg gtgatcgccg cgccggcgac cagcgtcgtg accgcggcgc
     1681 cggagatcag cgcccagcct ttgcgcaagg ttccccacaa taggcttacg tcgccaagcc
     1741 tctcccggct tcgcgcggag agccactcat acagcgccac aagggcgacc acggccgcaa
     1801 acgacatctc gaagctcgga tcgaacagcg attccggcgc gacgatgaga atggcgagcg
     1861 ccgccagggc gacgttgcgc atggtgagag ccggccggtc gagcatgacc gcgatcaaga
     1921 cgatgctcat catgatccag gaacgcaccg tgggcacggc ggcgcccgac agggcgagat
     1981 agaaggccgc ggcggcgagc gcggcggcgg cgctccactt cttgatcggg tagcgcagcg
     2041 caagcacggg caccagagcc agcagcgctc tcaccagcca gaacaccgtg ccggccatga
     2101 tcaccatgtg caggcc
//
LOCUS       DATNAE010000064         3438 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_4286189, whole genome shotgun sequence.
ACCESSION   DATNAE010000064 DATNAE010000000
VERSION     DATNAE010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4286189"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1600)
                     /locus_tag="VN518_01255"
     CDS             complement(<1..1600)
                     /locus_tag="VN518_01255"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HXE03231.1"
                     /translation="MPPKLCNWPAHPVFATASTKGEAKPAKTAESGGKVKLGKLPEWN
                     LTDLYQAPDSPKLKTDLETSERAADAMQERCAGKLAALLDGGKGGAHLAQAVREFEAL
                     NDVLGRVVSYASLLYAADTSDPKRQKFFGDIQEKITAISSKLLFFPLELNRLDDEMLE
                     TAMAKSELGHYRPWFEDLRKEKPYQLEDKLEQLFHEKAVTARGAWDRLFNETMTALRF
                     ETDGEKLSLEPTLNRLLDQDGNKRREAAQALAKVFKDNVRLFTLITNTLAKDKEISDR
                     WRGFVDVADSRHLANRVEKEVVDALVAAVRAAYPRISHRYYAMKARWLGKKTLEHWDR
                     NAPLPDEPKQVIGWDAAQKTVLTAYGDFSPRMADIAGQFFANGWIDAPVREGKSPGAF
                     SHPTVPSAHPYILLNYQGKPRDVMTLAHELGHGVHQVLANVQGPLMAPTPLTLAETAS
                     VFGEMLTFRKLLDQAPTPRERKALLASKVEDMINTVVRQIAFYSFERRVHTERREGEL
                     TSDRLGEIWLEVQRESLGPAIELKP"
     gene            1867..3375
                     /locus_tag="VN518_01260"
     CDS             1867..3375
                     /locus_tag="VN518_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXE03232.1"
                     /translation="MPKRVLVVDDDPAQRRILEEAIKRFGYSVATADSGDKALDALRA
                     DNTGEICLVLLDLVMPGTDGLAVLSAMRTLPRKPPAIVQTAHGSIDAAITAMRAGAVD
                     FVVKPVSPERLEVSIKNALKIEALAGELVRMKASARGELGFTDLIAGSDAMKRVLELG
                     HRASNSTIPVLIEGESGVGKELIARAIQGESARKSKSFVTVNCGAIPENLVESILFGH
                     EKGSFTGAVEKRIGKFLEADGGTLFLDEIGELPLDAQVKLLRALQDGEIDPVGGKHKV
                     KVDFRLISATNQNLISLVGEGRFREDLYYRLNVFPIWVPPLRERLEDVPELVRHFIAR
                     FAAEEGKRIDGIDADAIGMLCRYTWPGNIRQLENAVFRAVVLADAPMLSVNEFPQIAA
                     HVEGYAVTVPPAPAPKDPVPRIEGPVMLGESAGTPATIHVPQANGKDAIGIPALSDSG
                     DIRSLEAVEADMIRLAFGRYRGRMTEIARRLGIGRSTLYRKMREIGLEARPN"
BASE COUNT          596 a         1117 c         1093 g          632 t
ORIGIN      
        1 cgggcttgag ctcgatggcc gggccgaggc tctcgcgctg cacctcgagc caaatctcgc
       61 cgagccgatc gctggtcaac tcgccctcgc gccgctcggt gtgtacccgg cgttcgaaac
      121 tatagaacgc gatctgcctg accaccgtgt tgatcatgtc ctcgaccttg gaggcgagca
      181 gcgcctttcg ctcacgcggg gtcggcgctt ggtcgagcag cttgcggaag gtcaacatct
      241 cgccgaacac gctcgccgtc tcggccagcg tcagcggcgt cggcgccatc agcggccctt
      301 gcacattggc gagcacctga tgcacgccgt ggccaagctc atgcgccagc gtcatcacgt
      361 cgcgcggctt gccctgatag ttgagcagga tataggggtg cgcgctcggc accgtcggat
      421 gcgagaaggc gccgggagat tttccctcgc gcaccggcgc atcgatccag ccgttggcga
      481 agaactggcc ggcgatgtcg gccatgcgcg gcgagaaatc gccgtaggcc gtgagcacgg
      541 tcttctgcgc ggcgtcccag ccgatcacct gtttcggctc gtcgggcagc ggtgcgttgc
      601 gatcccaatg ctcgagcgtc ttcttgccga gccagcgcgc tttcatggcg taatagcgat
      661 gcgagatgcg cggataggcc gcgcgcacgg cggcgaccag cgcatcgacc acctccttct
      721 ccacccggtt ggcgaggtgg cgggagtcgg cgacgtcgac gaagccgcgc cagcgatcgg
      781 aaatctcctt gtccttggcc agcgtgttgg tgatcagggt gaacagccgc acattgtcct
      841 tgaacacctt cgccagagcc tgggcagcct cgcggcgctt gttgccgtcc tgatcgagca
      901 gccggttcaa ggtgggctcg aggctgagct tctctccgtc tgtctcgaat cgcagcgccg
      961 tcatcgtctc gttgaacagc cggtcccagg cgccgcgcgc cgtgaccgcc ttctcgtgga
     1021 atagctgctc gagcttgtcc tcgagctgat agggcttctc cttgcgcaag tcttcgaacc
     1081 agggccggta atggccgagc tcggacttgg ccatcgccgt ctcgagcatc tcgtcgtcga
     1141 gccggttaag ctcgagcgga aagaacagca gcttggagga aatcgcggtg atcttctcct
     1201 ggatgtcgcc gaagaatttc tgccgcttgg ggtcagaggt gtcagccgcg tagagcaggc
     1261 tggcatagga gacgacgcgg ccgagcacgt cgttcagcgc ctcgaactcg cgcacggctt
     1321 gcgcgagatg cgcgccgccc ttgccgccgt cgagcagcgc ggccaacttg ccggcacagc
     1381 gctcctgcat ggcgtcggcc gcccgctcgg aggtttcgag gtcggtcttg agcttcggcg
     1441 agtccggcgc ctgataaaga tcggtgagat tccattccgg caatttgccg agcttcacct
     1501 tgccccctga ctcggcggtc ttggccggct tcgcctcgcc cttggttgaa gcggtcgcga
     1561 aaacagggtg tgcgggccaa ttgcagagtt taggcggcat ggaaagggtg aacctcgctg
     1621 tcggtgaacc ggtctctagt gtgggagggc agacgcggcc ctgcaagggc catcgatcac
     1681 gtgttttggt ccttttcgag gaccgcgaga ccacggcatc accaaaatcg tacttcgata
     1741 aaggggatgt ttacccttcg actgcacaat tttggcacag gagcagcgtg cttcgaccgg
     1801 gtacatgttc gacgcgccgc tgtcctccgc atgtgggttg tagcgtaacg tagcgagtcc
     1861 agcgtcatgc ctaagcgcgt attggtcgtc gacgacgacc ctgctcaacg tcgtattctc
     1921 gaagaggcca tcaagcggtt cggctacagc gtcgccacgg cggatagcgg cgataaggcg
     1981 ctcgacgcgc tcagggccga caacacggga gagatctgcc ttgtcctgct cgacctcgtc
     2041 atgccgggca ccgacggcct agccgttctc tccgccatgc gcactttgcc ccgcaagcct
     2101 ccggccatcg tgcagaccgc tcatggcagc atcgatgccg ccatcacggc gatgcgcgcc
     2161 ggcgctgtcg acttcgtggt caagccggtg agtcccgagc ggctcgaagt ctccatcaag
     2221 aatgcgctga agatcgaggc gctggccggg gagctggtcc gcatgaaggc ctccgcccgc
     2281 ggcgagcttg gcttcaccga cctgatcgcc ggcagcgatg ccatgaagcg cgtgcttgag
     2341 ctcggccaca gggcttccaa ctccaccatc ccggtgctga tcgaaggcga atccggcgtc
     2401 ggcaaggagc tgatcgctcg cgccatccaa ggcgaaagtg cccgcaaatc caagtctttc
     2461 gtcaccgtca attgcggcgc catccctgag aatctggtcg agagcatcct gttcggccac
     2521 gagaaaggct ccttcaccgg cgccgtcgag aaacgcatcg gcaaattcct cgaagccgat
     2581 gggggcacgc tgttcctcga cgagatcggc gagctgccac ttgacgccca ggtgaagctg
     2641 ttacgcgccc tgcaagacgg cgagatcgat cccgtcggcg gcaagcacaa ggtcaaggtc
     2701 gacttccgcc tgatctcggc gaccaatcag aacctgatca gcctcgtcgg cgaaggccgc
     2761 ttccgcgagg atctctatta ccggctgaac gtattcccca tctgggtgcc cccgctcagg
     2821 gagcggctcg aggacgtgcc agagctggtg cgccacttca tcgcgcgctt cgccgccgag
     2881 gaaggcaagc ggatcgacgg catcgacgcg gacgccatcg gcatgctgtg ccgctatact
     2941 tggcccggca atatccgcca gctcgagaac gccgtgttcc gtgccgtcgt gctggccgac
     3001 gcaccgatgc tgtcggtgaa cgaattcccg cagatcgcgg cgcatgtcga gggctatgcc
     3061 gtgacggtgc cgccggcacc ggcgccgaag gaccccgtac cgcgcatcga aggtccggtg
     3121 atgctgggcg aatccgcagg cacccccgcc accattcatg tgccgcaggc caacggcaag
     3181 gacgcgatcg gcattccggc cctcagcgac tccggcgaca tccgctcgct cgaagccgtc
     3241 gaagccgaca tgatccgcct cgccttcggc cgctatcgag gcaggatgac ggagattgcc
     3301 cggcgcctcg gcatcggccg ctcgaccctc taccgcaaaa tgcgtgagat cggcctcgag
     3361 gcgagaccaa attaaccatc cgcatgggtt tgccgtgaat gccgcgcccg gagggaaagc
     3421 gtgacgggga aattttac
//
LOCUS       DATNAE010000065         7481 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_1274854, whole genome shotgun sequence.
ACCESSION   DATNAE010000065 DATNAE010000000
VERSION     DATNAE010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 7481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7481
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1274854"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1187)
                     /locus_tag="VN518_01265"
     CDS             complement(<1..1187)
                     /locus_tag="VN518_01265"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HXE03233.1"
                     /translation="MFSSGSDSRAALLQGLMIGALVVGTLYIAREVLLPLTLAILLSF
                     VLTPLLLLLRKIKVPRVLAVIIVVTLAFAIIFCLGWMMSQQASQLAGDLPRYQRVLAE
                     KIATLRKSAASSTTLEKAAGALKGIEDELNRSSAPPAPSAAPTPSLEDKNSNKPVLVQ
                     ITKPEPRAFEILQSVLGTVLPPLATAGIVILFVIFILLQREDLRDRLVRLMGASDMQR
                     ATATMNDAATRLSGYFLRQVLINSAYGFFIALGLWAIGIPSPMVWGILAMLMRFVPYV
                     GPFIAAAPPVLLGAVVDPGWTMMLLTGGLFLGSEFIMGQVVEPLVYGHGTGLSPIAVI
                     LSTVFWTWLWGPLGLLLAMPLTVCLVVLGRHVEGLNFLEVLLGDKPALTPAQSFYQRA
                     LTG"
     gene            1435..1668
                     /locus_tag="VN518_01270"
     CDS             1435..1668
                     /locus_tag="VN518_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03234.1"
                     /translation="MIRTLLASSFLAAGLLLAGSSQPAHAVWVCGPVQCVWDPTPDVG
                     YVAPAYAVTWGAPLYPGCYWKRGLLGRWRMICP"
     gene            complement(1730..2842)
                     /locus_tag="VN518_01275"
     CDS             complement(1730..2842)
                     /locus_tag="VN518_01275"
                     /inference="COORDINATES: protein motif:HMM:NF014345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HXE03235.1"
                     /translation="MIRKAAVLTLIAAFVSATTPGFACTGISLKAQDGAAIRGRTLEF
                     GFPMQSNVLVIPAGKEMSGTLPDGGKGLVYTSRYAIVGANALGLPAILDGLNDQGLSV
                     GLFYFPNYAKYTDVTPENAKHAIAPQEFGMWVLANFATVDEVKEGVKSIVVVPTPAPG
                     LGSPQGAVAGAHFFIQEKSGKSIVIEPVDGTLKVHDAPLGIMTNAPTYDWHMTNLQNY
                     INLSVKDVEGAKIGPVALAAFGSGSGLHGMPGDFTPPSRFVRAAIYSQSAAPNATAED
                     AVLSAFHILNQFDIPKGAVQNSAIGGTVDEITEWTSVADLKNLRWYFRTFTDQSIHVV
                     DLKEAVEAAKGEVRILKMEGTSQPITDASTNFVAGK"
     gene            complement(2943..3977)
                     /locus_tag="VN518_01280"
     CDS             complement(2943..3977)
                     /locus_tag="VN518_01280"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HXE03236.1"
                     /translation="MTAVGKTLERWRSEKTAAFLSSAVAAAEPDPAKAALFKDMEAAA
                     EEQAGILAQDLGRTPDFAPSLRSRLIAGCIAALGPRAMRPILSATKVRGVSVYSGKTE
                     APAGHPWPKTVEDVGGRHRTFGGGMLRAGVFGVNDGLVSNTCLVMGVAGAAAEPNIIV
                     LTGVAGLLAGAFSMAAGEFISMLSQREMFEHQISQEREELERYPKEEAEELALIYAAR
                     GIPLDEARDVASHLIANPEKALDTLAREELGLNPDDLGSPIGAAGSSFVAFAVGASLP
                     LIPFLLGTANGVAIAAAISGAALFAVGALLSLFSGKNAWLSGLRMLAIGAAAAAATYG
                     IGSLFNVSTS"
     gene            complement(3974..4705)
                     /locus_tag="VN518_01285"
     CDS             complement(3974..4705)
                     /locus_tag="VN518_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015933605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HXE03237.1"
                     /translation="MCELCGLADHRLSRRGLMAGASAALAASAFPSARLLAAEPPKEG
                     ASNAISPDAALQRIMEGNARYAANAPNERDFSAGRAARAGAQFPIAAILSCSDSRVVP
                     DLVFDQSPGDLFVVRLAGNFLDDDGLASLEYAVKFLGAPLLVVVGHTNCGAVAAAVKV
                     VTERAVLPGHLPELIKAIEPAVIAAHARHPSDLVAAAIEENVKLNVTRMKDDKPILSE
                     ALAAKNIAAYGGVYDIATGKVNLLS"
     gene            complement(4729..5889)
                     /locus_tag="VN518_01290"
     CDS             complement(4729..5889)
                     /locus_tag="VN518_01290"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXE03238.1"
                     /translation="MSSATAALDLFVNRLSGFSTPFSIELPSGEKRKIGLGEEEFHVG
                     LRNDRALKALRTLDEGDIAEAYLQGDIDLEGDMLKPFALRASLDDRHPLVAAWRFIEP
                     LLFGQVYTNKRAISSHYNADPKLFLSFLDPAFPAYSQGVYAHDDEPLAKALERKFDWV
                     IEKCELGPGKRVLEIGPGWGAFAGHALQAGVRFTGITNSEMSQSYLKGKLANFGDGAT
                     SGQFDVLLTDFYDFEPSEKFDSIVIMGVIEHLPNYERVLKKFYQLLKPGGFVFLDGSA
                     ARKKYELSTFMARHIYPGNHSFLVLDDLLNKLAKTEFELMEVHNDRWSYFLTFKQWAL
                     NLESNKDFIQRSFGDFEFRKFRLYLWGAAYEFLARNLDCYRLTLYKPKDAAA"
     gene            6125..7294
                     /locus_tag="VN518_01295"
     CDS             6125..7294
                     /locus_tag="VN518_01295"
                     /inference="COORDINATES: protein motif:HMM:NF015541.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXE03239.1"
                     /translation="MRFAAAGILAAFLSLGAGGAAKALYDVSPGEIPGKPGSIIRIWP
                     LEGGGPGMGGNGEAFRILYRSTDPSGRPIPVSGAIYIPNGPAPAGGRNIIAWAHPTSG
                     VVPPCAPSLMPDVGGMMWNLPNMIAEGYIVVATDYPGLGTDGIHPYLIGESEARSVLD
                     SVRAAREMPNTGASNRFAVWGHSQGGHAALFTGEVAARYAPDLKLVGVAAAAPATYLI
                     QLFDADAPTATGKDLTSMTLLSWSRLENVPVATLVVPQDMAAFEKVSHDCIESVAEFE
                     QIERDDQPLRTGDFIRIDPSEVEPWKGIMLRNSPGQAPAGAPVFIAQGTADTTVHPEI
                     TKRFGEALCTQGVRVSFVALPGVTHTFAARDSAAAALSWMTERFRGAPAPTSCER"
     gene            complement(7331..7480)
                     /locus_tag="VN518_01300"
     CDS             complement(7331..7480)
                     /locus_tag="VN518_01300"
                     /inference="COORDINATES: protein motif:HMM:NF018715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HXE03240.1"
                     /translation="MLISLVAGAFGYTGLARGAAQISKILFFIFLVLFIVILILAISA
                     GQLIF"
BASE COUNT         1428 a         2312 c         2466 g         1275 t
ORIGIN      
        1 tcgccggtca gcgcgcgctg gtagaagctc tgcgccgggg tcagcgccgg cttgtcgcca
       61 agcagcactt cgagaaagtt caagccctcg acatggcgcc caagcaccac gaggcatacg
      121 gtgagcggca tggcgagcaa caggccgagc ggcccccaca gccaggtcca gaacacggtg
      181 gagaggatca cggcgatggg cgaaagaccg gtgccgtgac cgtagacgag cggctccacc
      241 acctgcccca tgatgaactc cgagccgagg aacaggccgc cggtcaacag catcatagtc
      301 cacccgggat cgaccacggc gcccaacaga accggcgggg ccgccgcgat gaacgggccg
      361 acatagggaa cgaagcgcat cagcatggcg aggatgcccc acaccatggg gctcggaatg
      421 ccgatcgccc aaagcccgag cgcgataaaa aagccatagg ctgaattgat cagaacctgg
      481 cgcaggaaat aaccggagag acgcgtcgcc gcgtcgttca tggtggcggt ggcgcgctgc
      541 atgtcggagg cgcccatcag gcgcaccagc cggtcgcgca gatcctcgcg ctgaagcagg
      601 atgaaaatca cgaacaggat aacgattccg gcggtggcga gcggcggcag caccgtgccg
      661 agcacgctct gcaagatctc gaaggcccgc ggctctggct tggtgatctg gacgagaacg
      721 ggcttgttgg agttcttgtc ctcgaggctc ggagttggag cggcacttgg ggcgggcggc
      781 gcggaggatc ggttgagctc atcctcgatg cccttgagcg cgccggcggc cttctcgagc
      841 gtcgtcgacg acgccgccga tttgcgcaag gtcgcgatct tctcggcgag cacgcgctga
      901 tagcgcggca gatcgccggc aagctgagag gcctgctgcg acatcatcca gccaaggcag
      961 aagatgatgg cgaaggcgag agtgaccacg atgatcacgg caaggacgcg cggcaccttg
     1021 atcttgcgca ggaggagcaa aagcggcgtc agcacgaagc tcaacaggat ggcaagcgtg
     1081 agcggaagca ggacctcgcg cgcgatatag agcgtgccga cgacgagcgc cccgatcatc
     1141 agcccctgaa gcagcgcggc gcggctgtca gaacctgagg aaaacatcgg aaacggcgag
     1201 cgagacggca tggcggggct cccgtcacgc caagacgccc gaagctccaa aaaggcccgg
     1261 ccggcgcttg gcttctaccg gtttcgacat agcattgccg cccggacggg gccataacct
     1321 tgcgccccct ggaaagcttc ccgaaacgat gtggccgagt agggtatgga ccgggtgctc
     1381 aagcttgagc taaaccgctt ggtgcatccg tcagtgcaac taggaggact cgcgttgatt
     1441 agaacacttt tggccagttc atttctcgcg gccgggctgc tgcttgcagg ttcgagccag
     1501 ccggcgcacg ccgtatgggt ctgcggccct gtgcaatgcg tttgggaccc gaccccggac
     1561 gtcggctatg tcgcgccggc ctatgccgta acctggggag cgccgctcta tccgggatgc
     1621 tactggaagc gcggcttgct cggccgctgg cggatgatct gcccataggc ttcaagacaa
     1681 gcaatcggcc ggcgggttct tggcccgctg gcgattggac cgcgctaggt tacttccccg
     1741 cgacgaaatt cgtcgaggcg tcggtgatgg gttgcgaggt gccttccatc ttgaggatgc
     1801 gcacctcgcc cttggcggcc tcgaccgctt ccttcaagtc gaccacgtgg atcgactggt
     1861 cggtgaaggt cctgaaatac cagcgcagat tcttgagatc ggcgacgctc gtccattcgg
     1921 tgatctcgtc gaccgtgccg ccgatggccg agttctgcac cgcgcctttc gggatgtcga
     1981 actgattcaa gatgtggaag gcggagagca cggcgtcctc ggcggtcgcg ttcggcgccg
     2041 cagactggct gtagatcgcc gcgcgcacga agcgcgaggg cggggtaaag tcgccaggca
     2101 tgccgtgcag gcccgagcca gagccgaagg cagcaagcgc gaccggcccg atcttggcgc
     2161 cctcgacatc cttcaccgag agattgatgt agttctgcag attggtcatg tgccagtcat
     2221 aggtcggcgc gttggtcatg atcccgagcg gcgcgtcatg caccttcaag gtgccgtcga
     2281 cgggctcgat cacgatcgac ttgccgctct tttcctggat gaagaaatgt gcgccggcga
     2341 ccgcaccttg tggactgccg agcccaggcg cgggcgtcgg caccaccacg atcgacttta
     2401 ccccctcctt cacctcatcg acggtggcga aattggcgag cacccacatg ccgaattctt
     2461 gcggggcaat ggcgtgcttg gcgttctccg gcgtcacgtc ggtgtatttg gcgtaattcg
     2521 gaaaatagaa caggccgacg gaaaggccct ggtcgttgag cccatcgagg atggcgggaa
     2581 gcccgagcgc gttggcgccg acaatggcat agcgcgaggt gtagacgagg cccttgccgc
     2641 cgtcgggcag cgtgccgctc atctccttgc cggcggggat gacgagcacg ttcgactgca
     2701 tggggaagcc gaattcgagc gtgcggccgc ggatcgcagc accgtcctgc gctttcaggc
     2761 tgatgccggt gcaggcgaag cctggggtgg tggcagacac gaacgcggca atcagggtga
     2821 gaacagcggc tttgcggatc atgaggggga tctccggggt gcgattgccg gcagtctccg
     2881 cttagaccag tgaaagcccg cttaagcaga caatccgttt cgaattttag gagttcggtc
     2941 tctcaggagg tggagacgtt gaacagcgat ccgatgccgt aggtcgcagc ggcagcggcg
     3001 gcgccgatcg caagcatgcg caagccgctg agccaggcgt tcttgcctga gaacaggctg
     3061 agcagagccc cgaccgcaaa cagcgcggcg cctgagatcg ccgccgcgat ggcgacgccg
     3121 ttggcggttc ccaatagaaa cggaatgagc ggcaggctcg cgccgacagc aaaggcgacg
     3181 aaggacgaac ccgccgcgcc gatcggcgag ccgagatcgt ctgggttgag gccaagctcc
     3241 tcgcgcgcca gcgtgtcgag cgccttttca gggttggcga tcaaatggga agcgacatcg
     3301 cgggcttcat ccagcgggat gccgcgggcg gcgtagatca gcgcgagttc ttcagcttcc
     3361 tccttcggat agcgctctag ctcctcgcgc tcttgcgaga tctgatgctc gaacatctcg
     3421 cgctgcgaca gcatggagat gaactcgccg gccgccatgg aaaacgcgcc ggcgagcagc
     3481 ccggccacgc cggtgagcac gatgatgttc ggctcggccg cggcgccggc cacgcccatg
     3541 accaagcagg tgttggacac gagcccgtca ttcacgccga acacgccggc gcgcagcatg
     3601 ccgccgccga aggtgcgatg ccttcccccg acgtcctcga cggtcttggg ccaaggatgt
     3661 ccggctggag cctccgtctt gccgctatag accgagacgc cgcgcacctt ggtggccgaa
     3721 agaatggggc gcatggcgcg cgggccgagg gcggcaatac aaccggcgat gaggcgggag
     3781 cgtagcgacg gcgcaaaatc cggcgtgcgg ccaagatcct gagcgaggat gccggcctgc
     3841 tcttcggccg ccgcctccat gtctttgaac aaagcggcct tggccggatc gggctcggca
     3901 gcggcgacgg cagaggacag aaacgctgcc gtcttttccg accgccaacg ttccagcgtc
     3961 ttgccgacag cggtcacgag agcaaattca ccttgccggt ggcgatatcg taaactccgc
     4021 cataggcggc gatatttttc gcggcaagcg cctcggacag gatcggcttg tcgtccttca
     4081 tccgcgtgac attgagcttg acgttttcct caatggcggc ggccacgagg tcgctcggat
     4141 gccgggcatg ggcggcgatg acggcaggct cgatcgcctt gatcaactcg ggaaggtgac
     4201 ccgggagcac ggcccgctcc gtcaccacct tgacggcggc ggcgactgcg ccgcaattgg
     4261 tatgcccgac caccacgagg agcggcgcgc cgagaaattt caccgcgtat tcgaggctcg
     4321 cgaggccgtc gtcatcgagg aaattgccgg cgagccgcac cacgaagagg tcgccgggac
     4381 tttggtcgaa gacaaggtcc ggcacgaccc tgctgtcaga gcagctcaag atcgcagcga
     4441 tgggaaactg cgccccagcg cgggcggcac ggcccgccga gaaatcacgc tcgttcggcg
     4501 cattcgcggc gtagcgcgcg ttcccctcca taatgcgctg tagcgccgcg tcagggctga
     4561 tggcgttcga ggcgccctct ttcggcggct cagctgccaa taaacgggct gaagggaatg
     4621 cgcttgcggc aagtgccgca ctggcaccgg ccatgaggcc gcgacgggac aaacggtgat
     4681 cggctaagcc gcaaagctca cacatgggca aacctcttcg ttcggggttt aggcggctgc
     4741 gtccttgggc ttgtagagcg tgaggcggta gcaatcgaga tttcgagcga gaaactcgta
     4801 ggccgcgccc cagagataaa gtcgaaactt gcgaaattcg aaatcaccaa agctgcgctg
     4861 gatgaagtcc ttgttcgact caaggttaag cgcccattgc ttgaaggtga ggaaatagct
     4921 ccagcggtcg ttgtgcacct ccatcaactc gaactcggtc ttggcgagct tgttgagtag
     4981 gtcgtcgagc acgaggaagg aatgattgcc gggatagatg tggcgcgcca tgaacgtcga
     5041 cagctcgtat tttttccgtg ccgcgctgcc gtcgaggaac acgaagccgc cgggcttcaa
     5101 cagctgatag aacttcttca gcacccgctc gtagttgggc agatgctcga tcacgcccat
     5161 gatcacgatc gagtcgaact tctcgcttgg ctcgaagtcg tagaagtcgg tgagcaggac
     5221 gtcgaactgg ccactcgtgg ctccatctcc gaaattggca agcttgccct tgagatagga
     5281 ttgcgacatt tccgaattgg tgatgccggt gaagcgcacg ccggcctgca gcgcatgccc
     5341 ggcaaaggcg ccccaacccg ggccgatctc cagcacgcgc tttcccgggc caagctcgca
     5401 tttctcgatc acccagtcga acttgcgctc caaggccttg gcgagcggct cgtcgtcatg
     5461 ggcgtagacg ccctggctgt aggccgggaa cgccggatcg agaaagctca agaacagctt
     5521 cgggtcggcg ttgtagtgag aggagatggc gcgcttgttg gtatagacct gaccgaacaa
     5581 cagcggctcg atgaagcgcc atgccgccac cagaggatgg cgatcgtcga gcgaggcgcg
     5641 gagcgcgaac ggcttcagca tgtcgccctc aaggtcgatg tcgccttgca gataggcttc
     5701 ggcgatatcg ccctcatcga gcgtgcgcag cgctttcagc gcgcggtcgt tgcggagccc
     5761 gacgtgaaac tcctcctcgc cgaggccgat cttgcgcttc tctccgctcg gcagctcgat
     5821 ggagaacggc gtcgaaaatc cgctcaagcg attgacgaaa aggtcgagcg cggcggttgc
     5881 tgatgacatt cttgctcccc gaattttgct tgttccaact gacgatagcc gctcgcccgc
     5941 tcaaaaggcg gggcggtctc gcgctttcct ccgattgtgc cccggaagct tgggcaagct
     6001 caaggacagg atgcacgcct ccccctttac ctttgcccgg cttggcggtt gttgtccttg
     6061 gggggcgccg ttatcatccg cgccgccatc ataagggggg tcgcgtatga gagagtttgc
     6121 ctgcttgagg tttgccgccg ccggaattct cgccgccttt ctcagcctcg gcgccggcgg
     6181 cgcggcaaag gcgctttacg acgtaagtcc cggcgagatt ccgggaaagc ccggcagcat
     6241 cattcgcatt tggccgcttg agggcggtgg gcccggaatg ggcggcaacg gcgaagcctt
     6301 ccgcatcctc taccgctcga ccgaccccag cggccgtccg attcccgtat ccggcgcgat
     6361 ctacatccca aacggcccgg cgcccgccgg cgggcgcaac atcatcgcct gggcgcatcc
     6421 gacttccggc gtcgtgccgc cttgcgcgcc atcgctcatg cccgacgtcg gcggcatgat
     6481 gtggaacctg cccaatatga ttgcggaagg ttacatcgtc gtcgccaccg actatcccgg
     6541 cctcggcacc gatggcatcc acccttacct gatcggcgag agcgaagcac gatccgtgct
     6601 cgactcggtg cgcgcggcgc gcgagatgcc gaacaccggc gcgtccaatc gcttcgcggt
     6661 gtggggacat agccaaggcg gccacgccgc tctgttcacc ggagaggtcg ccgcacgcta
     6721 cgcgccggat ctcaagctgg tcggcgtggc ggccgcggcg ccggcaacct acctcatcca
     6781 attgttcgat gccgatgcgc cgaccgcgac cggcaaggat ctgacctcga tgacgctgct
     6841 ctcctggtca aggctcgaga acgtgccggt ggcgaccctg gtcgtgccgc aagacatggc
     6901 ggccttcgag aaggtctcgc acgactgcat cgaatcggtc gcagagttcg agcagatcga
     6961 aagagacgat cagccgctga ggacgggaga tttcatcagg atcgacccct cggaggtcga
     7021 gccttggaag gggatcatgt tgcgcaactc tccgggtcaa gctccggcag gcgcgccggt
     7081 gttcatcgcg caaggcaccg ccgacaccac ggtgcatccc gagatcacca agcgcttcgg
     7141 cgaagctctg tgcacgcagg gcgtgcgggt cagcttcgtg gcgctacccg gcgtgaccca
     7201 cacttttgcc gcgcgcgaca gtgctgccgc cgcgctcagt tggatgaccg agcgcttccg
     7261 cggggcgccg gcgcccacga gctgcgagcg gtaggtaggc ctcaccgcgg caggacgttt
     7321 cgtccaagcc tcagaagatg agctggccag cgctgatggc caggatcagg atcacgatga
     7381 agagaacgag gaagataaag aacagaatct tggagatctg cgcggcgccg cgcgcgaggc
     7441 cggtatagcc aaaagcgcca gcgacgagcg agatcaacaa g
//
LOCUS       DATNAE010000066         3312 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2176861, whole genome shotgun sequence.
ACCESSION   DATNAE010000066 DATNAE010000000
VERSION     DATNAE010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2176861"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1116
                     /locus_tag="VN518_01305"
     CDS             <1..1116
                     /locus_tag="VN518_01305"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HXE03241.1"
                     /translation="LGTLIAVLIYRLGAAGSAVLGGLAIAGVVGVSWYAFDVLGWLVD
                     PIYPAIALTAIYLAGTLFVFLRTERERNRVRHAFSHYMAPALVERLADDPSRLKLGGE
                     TRDMTLLFSDVRGFTTISEGLDAEELTRFLNSLFTPLSNIILDEQGTIDKFMGDAVMA
                     FWNAPLDDAAHPSHACSAALRMMAEMESLNARWREEAEAKGREFKPVRLGIGLNTGIC
                     CVGNLGSETRFDYSVIGDNVNVASRLEGQSKTYDVGTVVGQSTTARAPEFAFLELDLL
                     KVKGKTEATRIFALLGDNALKQSSDFIHLAERHTEFLGRFRAKDWEAAENLARECEQM
                     NTARLDHLYALYRERIAYFRINPPPANWDGTAEALSK"
     gene            1121..1516
                     /locus_tag="VN518_01310"
     CDS             1121..1516
                     /locus_tag="VN518_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HXE03242.1"
                     /translation="MPKLVLLALAAVAILMLKHTVADFYLQTPYQFLNKGKYGHPGGF
                     IHAGIHTALTPLVYLVLVPSSLLIAAGLALGEFVLHYHIDWLKEQVTHRNGWNTQDPG
                     FWYSLGTDQLVHGLTYLGIVAILVMVAGG"
     gene            complement(1513..>3312)
                     /locus_tag="VN518_01315"
     CDS             complement(1513..>3312)
                     /locus_tag="VN518_01315"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HXE03243.1"
                     /translation="GQWLRFIPYPVIGGFLAASGILLVTGGMEVVTQTNLTLSPSSWQ
                     VLYSPLYAPQILIGALFAASIPVLGRFVPNYLALPIAFFGFLLILDVSLFGFFNDATI
                     HSAWFLPSLGELTLWWPLTAMIGQQVDWGVIAQSSAEIGSFCGVMAIALLLDVSSLEV
                     ARQKTSDLDQEFRSNGLANLVASVFGGFGGSLSMNACLLLDESGATTRWSGAIVGIVC
                     AIILFSGTDVGSMVPKAILAGMLAYLGAVILIELWEAPAQSSWMEWALTGVMTLVIIN
                     FGYFMGVVVGVIGACLMFALSYSRIGVVRRHLTRQEFSSNVERSPEQTRLLREEGKRV
                     HVFWLSGFIFFGSSNGLFERIKRTIEAQREKPVGYVVLDFSAVPGLDTSAVLSLVKLR
                     NFCEENSVTLGFSGLSDIMRASFEKADFFGSERPHQVFGSRNEAIEWCEDMLLMYHEV
                     GAASTHSFESWLQSEFGEKVEFEKITPYMQLQELKSGEFLFRQGEPSDSVELQASGSV
                     AITIIDEHGRPIRLRRMIGHTVVGEMGFYRGVPRTASVIAEGPTVIYRLTREAFDTMQ
                     AENPTAAAALHKLIIRLLSDRLEFANREISALL"
BASE COUNT          639 a         1091 c         1007 g          575 t
ORIGIN      
        1 ctcggcacgc tgatcgccgt gctcatctat cgcctcggcg cggccggcag cgcggttttg
       61 ggcggtctcg ccatcgcagg cgtggtcggt gtctcctggt atgccttcga cgtgctcggc
      121 tggctggtcg accccatcta cccggccatc gcgctcaccg ccatctatct cgccggcact
      181 ctgttcgtgt tcctccgcac cgagcgcgag cggaaccggg tccgtcacgc cttctcgcat
      241 tatatggcgc cggctctggt cgaacggctg gcggacgatc cctcgcggct caagctcggc
      301 ggcgagacgc gcgacatgac gctcctcttc agcgacgtgc gcggcttcac caccatctcc
      361 gaggggctcg acgccgagga gctcacccgc ttcctgaact ccctgttcac gcccttgagc
      421 aacatcatcc tggacgagca aggcactatc gacaaattca tgggcgatgc ggtgatggcg
      481 ttttggaacg cgccgctcga cgacgcggcg catccgagcc atgcctgtag cgcggcgcta
      541 cgcatgatgg ccgagatgga gagcctgaac gcgcgctggc gcgaggaagc cgaagccaag
      601 ggccgcgagt tcaagccggt acggctcggc atcgggctca acaccggcat ttgctgcgtg
      661 ggcaatctcg gctcagagac gcgcttcgat tattcggtga tcggcgacaa cgtcaacgtc
      721 gcctcgcggc tcgaaggaca gtccaagact tatgatgtgg gcacggtggt cggccagtcc
      781 accaccgcgc gcgcgccgga gttcgccttt ctcgagctcg acctgctgaa ggtgaagggc
      841 aagaccgagg cgacccgcat cttcgccctg ctcggcgaca atgcgctcaa gcagtcgagc
      901 gacttcattc acctcgccga gcgtcacacg gagtttctcg gccgcttccg cgccaaggat
      961 tgggaggcgg cagaaaacct cgctcgcgag tgcgagcaga tgaacaccgc gcgcctcgac
     1021 cacctttatg cgctctatcg cgagcgcatc gcttatttcc gcatcaaccc gccgccggcc
     1081 aattgggacg gcacggccga ggccttgtcg aaatagtccg atgccaaagc tcgtcctgct
     1141 cgccctcgcc gccgtggcca tcctcatgtt gaagcatacg gtcgccgatt tttatctgca
     1201 gacgccgtat caattcctga acaagggaaa atacggccat ccgggcggct tcatccatgc
     1261 cggcatccat accgcgctga cgccgctggt ctacctggtg ctggtgccta gctcgctgct
     1321 aatagccgcc ggcctcgctc tcggcgagtt cgtgctgcac tatcacatcg attggctgaa
     1381 ggagcaggtc acccaccgca acggctggaa tacccaagac cccggcttct ggtattcgct
     1441 cggcaccgat cagctggtgc acgggctgac ctatctcggc atcgtcgcca ttctggtgat
     1501 ggtggccggg ggctagagca gcgcggagat ttcgcgattg gcgaattcga ggcggtccga
     1561 caacagcctg atgatgagct tgtgcagcgc cgccgcggcg gtcgggttct ccgcctgcat
     1621 cgtgtcgaac gcctcgcgcg tcagccggta gatcaccgtc ggcccctcgg cgatgacgct
     1681 cgccgtgcgc ggcacgccgc gatagaagcc catctctccc accaccgtat gcccgatcat
     1741 ccgccgcaaa cggatcggcc gaccgtgctc gtcgatgatg gtgatggcga ccgaaccgga
     1801 ggcttgcagc tccacggaat ccgacggctc gccttggcgg aagaggaact cgccgctctt
     1861 caattcctgg agctgcatgt atggcgtgat cttctcgaac tcgaccttct cgccgaattc
     1921 cgattgcagc cagctctcga agctatgcgt cgaggcggcg ccgacctcgt gatacatgag
     1981 cagcatgtcc tcgcaccact cgatcgcctc gttgcggctg ccgaatactt gatgcggccg
     2041 ctcgctgccg aagaagtccg ccttctcgaa gctcgcccgc atgatgtcgc tcagacccga
     2101 gaagccgaga gtgacgctgt tctcctcgca aaaattgcgg agcttgacca ggctcaacac
     2161 cgccgaggta tccagcccag gcacggcgct gaaatcgagc accacataac ccacgggctt
     2221 ctcccgctgc gcctcgatgg tgcgcttgat gcgctcgaac aggccgttgg aggagccgaa
     2281 gaagatgaaa ccggagagcc agaacacgtg aacgcgcttg ccctcctcgc gcaacagccg
     2341 cgtctgctcc ggcgagcgct cgacattgct ggagaattcc tgccgcgtca ggtgacgcct
     2401 gaccacgccg atacggctat agctcagcgc gaacatcagg caggcgccga tcacgccaac
     2461 caccacgccc atgaaatagc cgaaattgat gatgaccagc gtcatcacgc cggtaagcgc
     2521 ccattccatc cacgagcttt gcgcgggcgc ttcccacaac tcgatgagga tcaccgcgcc
     2581 gagataagcg agcatgccgg cgagaatggc tttcggcacc atgctgccga catcggtgcc
     2641 ggagaacaga atgatggcgc agacaatgcc gacaatggcg ccggaccagc gcgttgtggc
     2701 gccggactcg tcgagcagca ggcacgcgtt catcgacagc gatccgccga agccgccgaa
     2761 caccgacgcc acgagattgg caagaccgtt ggagcggaac tcctggtcga gatcgctggt
     2821 cttctgccgt gccacttcga ggctcgacac gtcgagcagg agcgcaatgg ccatcacgcc
     2881 gcagaatgaa ccgatctcgg cgctgctctg ggcgatcacg ccccaatcga cctgttggcc
     2941 gatcatcgcc gtcagcggcc accacaaggt caactcgccg aggctcggca ggaaccacgc
     3001 gctgtggatc gttgcgtcgt tgaagaagcc gaacaggctc acatcgagaa tgaggagaaa
     3061 gccgaaaaag gcgatcggca gggcgaggta gttcggcaca aaccgcccga gcaccgggat
     3121 ggaagcggcg aacagcgccc cgatcaggat ctggggtgcg taaagcggcg aatacagcac
     3181 ctgccagctg gagggcgaca gcgtgagatt ggtctgggtg acgacctcca tgccgccggt
     3241 gacgagcagg atgcccgaag cggcaagaaa gccgccgatc accgggtagg gaatgaagcg
     3301 cagccattgt cc
//
LOCUS       DATNAE010000067         3139 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_675118, whole genome shotgun sequence.
ACCESSION   DATNAE010000067 DATNAE010000000
VERSION     DATNAE010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675118"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /gene="recO"
                     /locus_tag="VN518_01320"
     CDS             <1..588
                     /gene="recO"
                     /locus_tag="VN518_01320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HXE03244.1"
                     /translation="AALQAGNSVQLTWRARLEEHLGTFALEVTDARAAALIADRTRLY
                     AAQLLCDHLRLLPERDPHDRLLGRVVILLDRTGVENLGAGLARFELMLLEELGFGLDL
                     SSCAVTGATTSLTHVSPKSGRAVSAEAAAPYADRLLALPAFLIDEDAPAMPADIAAAF
                     ALTGFFLDRHVWAARGIDPPATRDPLVEGLTAGEE"
     gene            688..2940
                     /gene="parC"
                     /locus_tag="VN518_01325"
     CDS             688..2940
                     /gene="parC"
                     /locus_tag="VN518_01325"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014130290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HXE03245.1"
                     /translation="MADSEILPPDEAGKQVVDLRQALEQRYLSYALSTITQRALPDAR
                     DGLKPVHRRILYGMRLLRLDPDGSYKKSAKVVGDVMGSFHPHGDQSIYDALVRLAQDF
                     SMRYPMVDGQGNFGNIDGDGAAAMRYTESRMTDVAMRLLEGIDESAVDFKPTYDGSSE
                     EPVVLPSNFPNLLANGSTGIAVGMATSVPPHNVAELCEAALHLINVNPNASAEELMRF
                     VPGPDFPTGGILIESPESIAQSYATGRGAFRLRARWNREEKGRGVYQIVVTEIPYGVQ
                     KSKLVEKIAELLLAKKLPLLKDVRDESADDIRLVLEPRAGTVDDALLMEQLFRLSELE
                     QRIPLNLNVLDKGSVPKVMSLAEALKAWLEHRKEVLVRRTRHRLDQIARRLEVLDGYI
                     VAYLNLDEVIRIIREEDEPKQVMIATFNLTDNQAEAILNMRLRSLRKLEEMELRQEHK
                     ELSEEQGHLNGLLASDKRQWGEISRQVNELKKVYGADTPLGRRRTALGEAPAHDVADI
                     EVSMIEREPITVILSEKGWIRALRGHTDDADEKGFKAGDKLKLLLKAETTDKLLLLTT
                     GGKVLTLAGDRLPGGRGQGEPVRLMVDIEEGQDIVDLFVYRPGQKRVVASLNGNGFIV
                     AEDELVANTRKGKQILNVSGDDEARLIVPAAGDMVAVIGQNRKLLIFAMDQLPEMARG
                     KGVRLQEYKDGGVADLKTFASAEGLSWTDSSGRTFVKTLDDLKDWIGERAQAGRQPPN
                     GFPRSNRFVG"
BASE COUNT          559 a         1061 c         1000 g          519 t
ORIGIN      
        1 gcggcgctgc aggccggcaa ttccgtgcag cttacctggc gcgcgcggct ggaggaacat
       61 ctcggcacct tcgctctcga ggtgacggat gcccgcgccg ccgcgctcat cgccgatcgg
      121 acacggctat acgccgccca gctcctctgc gaccacctgc gcctcttgcc ggagcgcgat
      181 ccgcacgacc ggctgctcgg gcgcgtggtg atcctgctcg accgcaccgg ggtggaaaac
      241 ctcggggctg ggctcgcccg cttcgagctg atgctgctgg aggagctcgg tttcggcctc
      301 gatctgagct cttgcgcggt gacgggcgcc accaccagcc tcacccatgt ttcgccgaaa
      361 tccggccgtg ccgtcagcgc cgaagccgcc gcgccttacg ccgaccggct gctcgccctt
      421 ccggccttcc tcatcgacga ggatgcgccc gcgatgccag ccgacatcgc cgccgccttc
      481 gcgctcaccg gcttcttcct cgaccgccac gtctgggcgg cgcgcggcat cgacccgccc
      541 gcgacccgcg acccgctggt ggaaggattg acggcggggg aagagtgaaa gcccgcggcg
      601 gggagacggt ggtcggaaac gccgtcagcc tgcagttgcc ttcaatgcgt tatagtccgc
      661 cgatgattcg aaacctgaag ttctgtcatg gccgattctg aaatcctgcc gcccgatgaa
      721 gcgggcaagc aggtcgtcga cctgcgccag gcgctcgaac agcgctatct ttcctacgcg
      781 ctctcgacca tcacccagcg cgccctgccc gatgcccgcg acggactgaa gccggtgcat
      841 cgccgtatcc tctacggtat gcgcctgctg cggctcgatc ccgatggttc ctacaagaag
      901 tcggcgaaag tcgtcggtga cgtgatgggc tcgttccatc cgcacggcga tcagtcgatt
      961 tacgacgccc tggtccgcct cgcgcaggat ttttccatgc gctacccgat ggtcgacggg
     1021 cagggcaatt tcggcaatat cgacggcgat ggcgccgccg ccatgcgcta taccgaaagc
     1081 cgcatgaccg acgtcgcgat gcgtctgctc gaaggcatcg acgagagcgc cgtcgacttc
     1141 aagccgacct atgatggctc gagcgaagag ccggtcgtcc tcccgtcgaa ttttccgaac
     1201 ctgctcgcca atggttcgac cgggatcgcc gtcggcatgg cgacctccgt gccgccgcac
     1261 aacgtcgccg agctttgcga ggcggcgctc cacctgatca acgtcaaccc caacgcctcc
     1321 gccgaagagc tgatgcgctt cgtgcccggg ccggatttcc cgaccggcgg tatcctgatc
     1381 gaaagcccgg agtcgatcgc ccagtcctac gccaccggtc gcggtgcctt ccgcctccgc
     1441 gcgcgctgga atcgggagga aaagggccgc ggcgtctatc agatcgtcgt caccgaaatc
     1501 ccctacggcg tgcaaaaatc caagctcgtc gagaagatcg ccgagctgct gctggccaag
     1561 aagctgcccc tgctgaagga tgtgcgcgac gaaagcgccg acgacatccg cctcgtgctg
     1621 gagccgcgcg ccggtaccgt tgatgacgca ttgctgatgg agcagctctt ccgcctctcc
     1681 gagctcgaac agcgtatccc gctcaacctc aacgtgctcg ataagggcag cgtccccaag
     1741 gtgatgagcc tcgccgaggc gctgaaggcc tggctcgagc accgcaagga ggtgctcgtc
     1801 cgccgcaccc gccaccggct cgaccagatt gcccgccggc tcgaggtgct cgacggctat
     1861 atcgtcgcct acctcaatct cgatgaggtg atccggatca tccgcgagga ggacgagccc
     1921 aagcaagtga tgatcgccac gttcaacctc accgacaatc aggccgaggc gatcctcaac
     1981 atgcgcctgc gctcgctccg caagctcgag gaaatggagc tgcggcagga gcacaaggag
     2041 ctctccgagg agcaggggca cctcaatggg ttgctcgcct ccgacaagcg gcaatggggc
     2101 gagatctcca ggcaggtcaa tgagctgaag aaggtttatg gcgccgatac gccgctcgga
     2161 cgccgccgca cggcgctggg tgaggcgccg gcgcacgacg tcgccgacat cgaggtgtcg
     2221 atgatcgagc gtgagccgat cacggtgatc ctctctgaga agggctggat ccgcgcgctc
     2281 cggggccaca ctgatgacgc cgacgaaaag ggcttcaagg ccggcgacaa gctgaagctt
     2341 ctgctcaagg ccgagacgac cgacaagctg ctattgctga cgacgggcgg caaggtgctc
     2401 accctcgccg gtgatcgcct gccgggcggg cgcgggcagg gcgagccggt gcgtctcatg
     2461 gtcgatatcg aggagggaca ggatatcgtc gatctcttcg tctatcggcc cggccagaag
     2521 cgcgtcgttg catcgctcaa cggcaatggc ttcatcgtcg ccgaggacga gctcgtcgcc
     2581 aatacccgca agggcaagca gatcctcaat gtttcgggcg acgacgaagc gcgactgatc
     2641 gttccggcgg cgggcgacat ggtcgcggtc atcggccaga accgcaagct cctgatcttt
     2701 gcgatggacc agttgccgga aatggcccgc ggcaagggcg tccgcctgca ggaatacaag
     2761 gacggcgggg tggcggacct caagaccttc gcctcggccg aaggattgtc ctggaccgac
     2821 agctccggcc ggaccttcgt gaagactctc gatgacctca aggactggat cggcgaacgc
     2881 gcccaggccg gccgccagcc tccgaacggt tttccgcgga gcaacaggtt cgtggggtaa
     2941 ggggcaagtc gataatcgac gcctttggca aggccccctc acccggcctt cggccgacct
     3001 ctcccccaag ggagaggtga cgatcgggca cggctttgcc tccgcttcac ctctcccttg
     3061 gggagaggtc gggcttcgcg aagcgaagac cgggtgaggg ggccttacgg caagctaaac
     3121 ctccagcggc cgccagccg
//
LOCUS       DATNAE010000068         3128 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2484746, whole genome shotgun sequence.
ACCESSION   DATNAE010000068 DATNAE010000000
VERSION     DATNAE010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2484746"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..2389
                     /locus_tag="VN518_01330"
     CDS             50..2389
                     /locus_tag="VN518_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HXE03246.1"
                     /translation="MTDPFRLDEEFTPRPGGIAARARAAVAAPQPYLSGLNPEQRRAV
                     EATDGPVLVLAGAGTGKTRVLTTRIAHLIATGKARPFDILGVTFTNKAAREMKERVAH
                     LVGPAAEGMPWLGTFHAIGVKILRRHAELVDLKPSFTVLDTDDQIRLLKQLLEAENID
                     EKRWPARLLAAMIDGWKNRGLTPKRVPEGESFAYANGKGRELYAAYQHRLKELNAADF
                     GDLLLENLRLFQDHPDVLAQYQTRFQFMLVDEYQDTNVAQYLWLRLLAQGSRNICCVG
                     DDDQSIYGWRGAEVDNILRFETDFPGAKIIRLERNYRSTANILGAASGLIAHNQGRLG
                     KTLRTDGADGEKVEVQGCWDDEEEARVIGEDIEQLQRQGQNLSEIAILVRASFQMRAF
                     EDRFISLGLPYRVIGGPRFYERQEIRDATAYFDVTLNPANDLKFERIANTPRRGLGES
                     SLKSLHQLARAHNVPLMQAARLIVETEELKPKSRKALADLIAAFDRWRSMIDTMRHTE
                     LAELILDESGYTAMWQAEKSPQAQSKLENLKELIRFMEEFDTLAGFMEHVSLVMDAES
                     EESTERVNLMTLHSAKGLEFDVVFLPGWDEGLLPHQRSLDESGRSGLEEERRLAHVGL
                     TRARVKAKITFAQNRRTHGMWQQAIPSRFIDELPIAHVEVAETQGFHGSYGKSRFDTA
                     ENLFASGFYETPGWQRAQRNRPPGSTPASPRAREPITIDGTLVAASTVEGAGLSVGMR
                     VFHQKFGYGRIAQVDGNKLTVDFDKAGQKKVVDSFVERC"
     gene            2402..>3128
                     /locus_tag="VN518_01335"
     CDS             2402..>3128
                     /locus_tag="VN518_01335"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HXE03247.1"
                     /translation="MSARIKTEPVTFIATIEAPASSAETIAAGLEAAESPAALAVTLF
                     ECGQDRIEVSAHYASEPSRNLLTRLIEAAAFDGRLGALRIEPLPQKNWVAEAESLRGP
                     VRAGRFLVHGRHDRGKVPPGRFTLEIDAGLAFGTAHHATTRGCLIALDRLLKLDRPAC
                     VLDVGTGTGILAIAAAKALGRPVVASDMDPVAVAVAAENASRNCARSRILVVEAEGLA
                     HRALRYAKADLLFANLLLRPLLEL"
BASE COUNT          570 a         1074 c          977 g          507 t
ORIGIN      
        1 ctcatgtgag cgctcctggc cggaaccgca ctctcggaag ccacggaccg tgaccgaccc
       61 atttcgcctg gacgaagagt tcactccccg cccgggcggc atcgcggcgc gcgcccgcgc
      121 ggccgttgcc gcgccccagc cctatctgtc cggcctcaac ccggaacagc gccgcgcagt
      181 ggaagccacc gacggccccg tgctggtgct ggccggcgcg ggcaccggca agacgcgcgt
      241 gctcacgacc cgcatcgccc acctgatcgc cacgggcaag gcgcgcccct tcgacatcct
      301 cggcgtcacc ttcaccaaca aggcggcccg cgagatgaag gagcgggtgg cccacctcgt
      361 ggggcccgcc gccgagggca tgccctggct cggcaccttc cacgccatcg gcgtcaagat
      421 cctgcgccgt catgccgagc ttgtggacct caagccgtcc ttcacggtgc tcgacaccga
      481 cgaccagatc aggctgctga agcagctgct cgaagcggag aacatcgacg agaagcggtg
      541 gccggcgcgg ctgctcgccg ccatgatcga cgggtggaag aaccgcgggc tcacgccgaa
      601 gcgcgtaccg gaaggcgaat cgtttgccta tgccaacggc aagggccgcg agctttatgc
      661 cgcctatcag caccggctga aggagctgaa cgccgcggat ttcggcgatc tcctgcttga
      721 gaatctgagg ctgttccagg accaccctga cgtgcttgcg caatatcaaa cgcgctttca
      781 gttcatgctg gtcgacgaat atcaagacac caacgtcgcc caatatttat ggctcaggct
      841 gctcgctcaa gggagccgca atatctgctg cgtcggcgac gacgaccagt ccatctacgg
      901 ctggcgtggg gcagaggtgg acaacatcct ccgcttcgag accgacttcc ccggcgccaa
      961 gatcatcagg ctcgagcgca actaccgctc taccgccaac atcctcggcg ccgcctcggg
     1021 cctcatcgcc cacaatcagg gtcgtctcgg caagacgctg cgcaccgacg gcgccgacgg
     1081 agagaaggtc gaggtgcaag gctgctggga tgatgaggaa gaagcccgcg tcatcggcga
     1141 ggatatcgag cagctgcagc ggcaagggca aaatttgagc gagatcgcca tcctcgtgcg
     1201 agcgagcttc cagatgcgcg ccttcgagga ccgcttcatc agcctcggcc tcccctaccg
     1261 cgtgatcggc ggcccacgct tctatgagcg ccaggagatc agggatgcca ccgcttattt
     1321 cgacgtgacg ctcaatccgg ccaacgatct gaaattcgaa cgcatcgcca atacgccgcg
     1381 gcgtggcttg ggcgaatcga gcctcaaatc gctccaccaa cttgcccgcg cgcataatgt
     1441 gccgctgatg caggcagcac ggctgattgt cgagaccgag gagctgaagc cgaaatcgcg
     1501 caaggccctt gccgacctga ttgccgcgtt cgaccgctgg cgctcgatga tcgacaccat
     1561 gcgccatacc gagctcgccg agctgatcct cgatgagtcc ggctacaccg ccatgtggca
     1621 ggctgagaag tctccgcagg cgcagtcgaa gctcgaaaac ctgaaagagc tgatccgctt
     1681 catggaggag ttcgacactt tggccggctt catggagcat gtcagcctgg tgatggacgc
     1741 ggaaagcgaa gagagcaccg agcgcgtcaa tctgatgacg ctgcacagcg ccaaggggct
     1801 cgaattcgac gtcgtgttcc tccccggctg ggacgaagga ctcttgccgc atcagcgaag
     1861 cctcgatgag tccggacgct caggcctcga ggaagagcgc aggctcgccc atgtcgggct
     1921 gacccgggca agggtcaagg ccaagatcac cttcgcccag aaccgccgca cccacggcat
     1981 gtggcagcag gcgatcccgt cacgcttcat cgacgaattg cccatcgccc atgtcgaggt
     2041 cgccgagacc caaggcttcc atggcagcta cgggaagagc cgcttcgaca cggcggagaa
     2101 cctctttgcc agcggattct acgagacgcc cggctggcag cgcgcgcaac ggaaccggcc
     2161 gcccggaagc acgccggctt caccacgcgc acgcgagccg atcaccattg acggcacgct
     2221 cgtcgcggcg agcacggtcg agggcgccgg gctctccgtc ggcatgcgcg tgttccacca
     2281 gaagttcggc tatggccgca tcgcgcaggt cgacggcaac aagctcaccg ttgatttcga
     2341 caaggccggc cagaagaagg tggtggacag cttcgtcgag cgctgctgac cagctcgcgc
     2401 catgtcggca cgcatcaaga ccgagcccgt taccttcatc gcgaccatcg aggcgcccgc
     2461 ctcaagcgcg gagaccattg ctgccggact tgaggccgcc gagagccctg ccgctttggc
     2521 cgtgaccctg ttcgagtgcg gtcaagaccg gattgaggtc tcggcgcatt acgcgagcga
     2581 gccctcgcgc aatcttctga cgcggttgat cgaggccgcg gccttcgatg gccgtcttgg
     2641 cgccctccgg atcgagccgc tgcctcagaa gaactgggtg gcggaagccg agagcttgcg
     2701 ggggccggtg cgcgccgggc gcttcttggt gcatggccgt cacgaccggg gcaaggtgcc
     2761 cccggggcgc ttcacgcttg agatcgacgc cggccttgcc ttcggcaccg cccatcatgc
     2821 caccacccgc ggctgcctca tcgcgctcga tcgcctgctc aagctggatc gcccggcctg
     2881 cgtgctcgat gtcggcaccg gcaccggcat cctcgccatc gccgccgcca aggctcttgg
     2941 ccgacctgtg gttgcgagcg acatggaccc ggtcgccgtc gctgtcgccg ccgaaaatgc
     3001 ctcaaggaac tgcgctcgct cgcgtatcct cgtggtggag gctgaggggc ttgcccatcg
     3061 ggcgttgcgc tacgccaagg ccgacctgct cttcgccaac ctgctgcttc gtcctctgct
     3121 cgagcttg
//
LOCUS       DATNAE010000069         5332 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2179025, whole genome shotgun sequence.
ACCESSION   DATNAE010000069 DATNAE010000000
VERSION     DATNAE010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5332
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2179025"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..1502
                     /locus_tag="VN518_01340"
     CDS             165..1502
                     /locus_tag="VN518_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXE03248.1"
                     /translation="MTATRKILELNAFDMRAAAGLDPETADLVARRRRVFGPTSMLFY
                     ARPLNLVRGEGVWLIGADGTRYLDAYNNVASVGHCHPRVVAAVSRQMAILNTHSRYLS
                     EIVYTYAERLLATLPRELSNIVLTCTGSESVDLALRIARAVTGGTGFIVTENAYHGNT
                     LAVTEISPSSASAEPRNPNVFLVPAPDTYRTGPEEVGPLFAANIRKAISAMKKKKIRF
                     AGLVADSIFSSDGIYPGEPGFMAEAIADVRKEGGLFIADEVQPGFARTGETMWGFERH
                     GLVPDMVVMGKPMGNGYPIAGLAVKPDLLATFGKTAGYFNTFGGNPVAAAAGLAVLET
                     LEAENLRENALEVGAYLLAGLGAVAQRFSQVGDVRGKGLYIGVEFVRDRKSKTPDRAA
                     ATSVVEMLRDRNILVGIAGPYGNIVKIRPPLCFARDHADLLIKALSDSLATLS"
     gene            1605..2372
                     /locus_tag="VN518_01345"
     CDS             1605..2372
                     /locus_tag="VN518_01345"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016554378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXE03249.1"
                     /translation="MPGRLAGKIALISGGATGMGGASSKLFAAEGAKVGVVDRNEEAG
                     RAVVAEIEAAGGTAMFAAADVANEGEVNAAVAAVTAALGAPNVLFNHAGSIIIKPFLE
                     TTLADWEWLMAVNVTSMFLVTKAVLPGMIAAGGGSIVCTSSISAIAATPREVVYNTSK
                     GACHMFARSIAVEFRDQSIRCNAVCPGFVRTPHGLREVAELNKLGVDMSDAALAVQQG
                     RICEPEEVARAALFLASDEASFVNGAHLFVDNCFTAV"
     gene            complement(2403..2906)
                     /locus_tag="VN518_01350"
     CDS             complement(2403..2906)
                     /locus_tag="VN518_01350"
                     /inference="COORDINATES: protein motif:HMM:NF014892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HXE03250.1"
                     /translation="MSKQDKKHTGTFRQDKLMPDETLEAQLDGWLDKPTGQGGVIKHN
                     GQFVLTNKRACFYSKAPFEEIFETIPLSKITSVETSSLMGYRVLRLHTAHDDLEFKTL
                     EAKALFDTLLAHLERLRHEPAGGSVAPAASESITDQIKKLGELRDAGFLTNDEFNAKK
                     AELLARL"
     gene            complement(3134..4552)
                     /locus_tag="VN518_01355"
     CDS             complement(3134..4552)
                     /locus_tag="VN518_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HXE03251.1"
                     /translation="MGKITGFLEIERQDRHYEPAADRVRHYREFLVPLGDAEVTQQAA
                     RCMDCGIPYCHQGCPVNNQIPDWNDLVYHADWQEAASNLHSTNNFPEVTGRVCPAPCE
                     ASCTLNIIDVPVTIKSIECAIADKAFAAGWVRPQPPETKTGKRVAVVGSGPAGLAAAQ
                     QLARAGHDVHVYEKHAQPGGLLRYGIPDFKMEKYLVERRVEQMQAEGVTFHCNMHVGH
                     DISARKLERDYDAVLLAGGAEHARDLTIEGRDLRGVHYAMNFLPQQNRRVSGEPLGDV
                     EPILATAKQVVVIGGGDTGSDCIGTSFRQGAVSVTQLEIMPMPPEKENKALTWPNWPL
                     KLRTSSSQAEGAARDFSVATSRFLGENGEVKELECIRLDAQMKPMPGSEFRIPADLVL
                     LAMGFLHPVQEGMIKDLRINLDPRGNVKANELDYRTSRPKVFAAGDMRRGQSLVVWAI
                     REGRQAAHAIDKFLMGKTDLPR"
     gene            complement(4700..>5332)
                     /locus_tag="VN518_01360"
     CDS             complement(4700..>5332)
                     /locus_tag="VN518_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010970207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit alpha"
                     /protein_id="HXE03252.1"
                     /translation="DSGIVPEESIIVGNTVLYGAIAGECYFRGIGGERFAVRNSGATA
                     VVEGTGDHGCEYMTGGVVVVIGPTGRNFAAGMSGGIAYVLDEAGDFERRCNLAMVELE
                     PVPSENNVMERSRHQTGDLESHGLVDVSDMTRFDEQRLYQLIERHLHYTGSERARAIL
                     ADWSAYLPKFVKVMPVEYRRALEEIARQQVLDRTALGEIEIGLRAGANGR"
BASE COUNT          913 a         1706 c         1730 g          983 t
ORIGIN      
        1 attctacaga aggccctgca acctgcccga gggtcgctgg ccggccgtcc gcggcgcccc
       61 cggcggtctc ccggaaaaaa ttgaacgatg gagaccggct gatctaggct agaggtgagc
      121 ggagccaggg tgcgtccaca atgccgcatg gctcaaagga tgtcatgaca gcgacgcgca
      181 aaattctcga acttaacgcc ttcgacatgc gagcggccgc cggtctcgat cctgaaacgg
      241 ccgacctcgt cgcccgccgc aggcgcgtat tcggtccgac atcgatgcta ttctatgcgc
      301 gccccctcaa tctcgtccgc ggggaaggcg tctggctcat cggtgccgac ggcacgcgct
      361 atctcgacgc ctacaacaac gtagcgtctg tgggccactg ccatccccgc gtcgtcgccg
      421 ccgtgtcgcg gcagatggca atcctcaaca cgcacagccg ctacctcagc gagatcgtct
      481 acacctatgc ggaaaggctg ttggcgacgc tgccgcgcga gctgtccaat atcgtcctga
      541 catgcacggg aagcgaaagc gtcgacttgg cgctgcgcat cgcacgcgcg gtcaccggcg
      601 gcacgggctt catcgtgacg gagaatgcct atcacggcaa tacactggcc gtgaccgaaa
      661 tctccccctc gtcggcgagc gccgagccgc gcaatccgaa cgtctttctg gttccagccc
      721 ccgacaccta tcgaacgggg ccggaggagg tggggcctct cttcgcggcc aacatcagaa
      781 aggcgatttc ggccatgaag aaaaagaaaa tccgcttcgc cgggctcgtg gccgacagca
      841 tcttctccag cgacggcatc tacccgggcg agcccggatt tatggccgaa gccatcgcgg
      901 atgtgcgcaa agaaggtggc ctgttcatcg ccgacgaggt gcagccaggc ttcgcccgca
      961 cgggcgaaac catgtggggg tttgagcgtc acggcctcgt tccggacatg gtcgtcatgg
     1021 gcaagcccat gggcaacggc tatcccatcg ccgggctcgc ggtgaaaccg gatctgctcg
     1081 cgaccttcgg gaaaactgcc ggctacttca acacgttcgg aggaaacccg gtcgccgccg
     1141 cggccggcct cgccgtcttg gagacgcttg aggccgagaa cctgcgagag aacgcgctcg
     1201 aggtcggcgc ctatctcttg gcggggctcg gcgccgtcgc ccagcgcttc agccaagtcg
     1261 gggacgtgcg gggaaaaggg ctttacatcg gcgtcgagtt cgtgcgcgac cgcaagtcga
     1321 agacgccgga cagggccgcc gcaaccagcg tcgtggaaat gctccgcgac cgcaacatcc
     1381 tggtcggcat tgccggcccc tacggcaaca tcgtcaagat ccgcccgccg ctctgctttg
     1441 cgcgggacca tgccgacctt ctcatcaagg cgctttccga ctcgcttgcg actctctcct
     1501 gatcgccggg gacgagagcg ctccgccccg cttgccactt tcggccgcgc ccggcgccgt
     1561 tctagaatgg ccgaccgtct ctagagaaga aggatgaacg caccatgccc ggaagactgg
     1621 caggcaagat tgcattgatt tcgggcggcg cgaccggcat gggaggcgcc tcctcgaagc
     1681 tgttcgcggc cgagggcgcc aaggtcggcg tcgtcgaccg caacgaggag gcggggcgtg
     1741 ccgtcgtagc cgagatcgaa gcagcgggcg gcacggctat gtttgcggcc gcggatgtcg
     1801 ccaacgaagg agaggtcaac gcggcggttg cagccgtcac cgcggcgctc ggcgcgccaa
     1861 acgtgctgtt caatcacgcc ggctccatca tcatcaagcc cttcctcgag accacgctcg
     1921 ccgactggga atggctgatg gcggtcaacg tcacgagcat gttcctcgtg accaaggccg
     1981 tgctgccggg gatgatcgcg gccgggggag ggtcgatcgt gtgcacctcc tcgatctcgg
     2041 ccatcgccgc gacgccgagg gaggtggtct acaatacgtc gaagggtgcc tgccacatgt
     2101 tcgctcgctc gatcgcggtc gagtttcgcg accagagcat ccgctgcaac gccgtctgcc
     2161 cggggttcgt gcgcacgccg cacggcctgc gcgaggttgc cgagctcaac aagcttggcg
     2221 tcgacatgtc ggatgcggcc cttgccgttc agcagggtcg catttgcgag ccggaggagg
     2281 tggcgcgcgc tgcgctgttt ctcgcgagcg acgaggcgag cttcgtcaac ggcgcgcatc
     2341 tcttcgtcga caactgcttt accgcggtgt gagcctggct cccgccgaag cggtgcctat
     2401 cctcataacc tcgcaagaag ctcggccttc ttggcgttga actcgtcgtt ggtcaggaat
     2461 ccggcgtcgc gaagctcacc gagtttcttg atttggtcag ttatagattc tgacgcggcc
     2521 ggagcaacgg agccgccggc cggctcgtga cgaaggcgtt cgaggtgcgc aaggagggtg
     2581 tcgaagagag ctttggcctc gagggtcttg aattcgagat cgtcatgcgc cgtgtgaaga
     2641 cggagcacac ggtagcccat cagcgacgac gtctcgaccg acgttatttt cgagagtggg
     2701 atcgtctcga atatctcctc gaacggagcc ttggaataga agcaagctcg cttgttggtc
     2761 agcacgaact gaccgttatg cttgatcacg ccgccttggc cggttggctt gtcgagccag
     2821 ccgtcgagtt gggcttcgag cgtctcgtcc ggcataagct tgtcttgtcg aaaagtgccg
     2881 gtatgttttt tgtcctgctt gctcatgccc ctcttctccc ctcattccat gaaggctttt
     2941 ccgtccgcag gagcctgagg ggcttcaccc ccttggaatt gcttagtccg atcgtgcttg
     3001 ggcgaggact cgggctgcaa tagtttaaac agcaccctgt ctcatcaaca cacattacag
     3061 caataaaggg cgccgaccaa agcgcgacac cctctcttgg aggtggtctt tggccgcgtc
     3121 agccgggcct tcgtcagcgc ggcaggtcgg tttttcccat caggaatttg tcgatggcgt
     3181 gcgccgcctg gcggccttcg cggatggccc acaccacaag cgactgaccg cggcgcatgt
     3241 cgccggcggc aaacacctta ggccgcgagg tgcggtagtc gagctcgttc gccttcacat
     3301 taccgcgcgg gtcgaggttg atccgcaagt ccttgatcat gccctcctgc acgggatgca
     3361 ggaagcccat ggcgagcagc acgaggtccg cgggaatgcg gaactcgctg ccgggcatcg
     3421 gcttcatctg ggcgtcgagg cggatgcatt cgagctcttt gacctcgccg ttctcgccca
     3481 agaagcggct cgtggccacg ctgaagtcgc gtgcggcgcc ctcggcctgg ctcgatgagg
     3541 tgcggagctt gagcggccag tttggccagg tcagcgcctt gttctccttc tccggcggca
     3601 tcggcatgat ctcgagctgg gtcaccgaga ccgcgccttg gcggaaggag gtgccgatgc
     3661 agtcggagcc ggtatcgccg ccgccgatca ccacgacttg cttggctgtg gcgaggatcg
     3721 gctcgacgtc gcccaacggc tcgccagaga cgcggcggtt ctgctgcggc aggaaattca
     3781 tcgcataatg cacgccgcgg aggtcacggc cttcgatggt aagatcgcgc gcatgctcgg
     3841 cgccgccggc gagcagcacc gcgtcgtagt cgcgctcgag cttgcgcgcc gagatgtcgt
     3901 ggccgacatg catattgcag tggaaggtca cgccctcggc ttgcatttgc tccacgcggc
     3961 gctcgacgag gtatttctcc atcttgaagt cggggatgcc gtagcggagc aggccgccgg
     4021 gttgggcgtg cttctcatag acatgcacgt cgtggccggc gcgggcgagc tgctgggccg
     4081 cggcgagccc ggcggggccg gagccgacaa ccgcgacgcg cttgccggtc ttggtctcgg
     4141 gaggctgcgg cctcacccag cccgcggcaa aggccttgtc ggcgatggcg cattcgatcg
     4201 atttgatggt gacggggacg tcgatgatgt tgagcgtgca ggaggcctcg cacggtgcgg
     4261 ggcagacgcg tcccgtcacc tcggggaaat tgttggtgga atggaggttg ctcgccgcct
     4321 cctgccagtc ggcgtggtag acgaggtcgt tccagtcagg gatctggttg ttgaccgggc
     4381 agccctggtg gcaatagggg atgccgcaat ccatgcagcg ggcggcctgc tgtgtcacct
     4441 cggcatcgcc gagcggcacg aggaactcgc gataatggcg gacgcggtcg gccgccggct
     4501 cgtagtgccg gtcctgccgc tcgatctcga gaaagcccgt gatctttccc atcaacccat
     4561 ccgctcgttc ggcgctttcc ccgccgttcc taaaagtgtc gccagcagac cctctgctcg
     4621 tcaagccgtc ctgagccgag agggccagcc ttcccgcccc cagacgaggc gaggaagcgt
     4681 aaatcgcctc cggttaagat tatctgccgt tggcgccggc gcggagaccg atctcgatct
     4741 cgccgagggc cgtcctgtcg agcacctgct ggcgtgcaat ctcctcgagc gcccggcgat
     4801 actcgaccgg catcaccttg acgaatttcg gcagataagc gctccaatcg gcaagaatgg
     4861 cgcgggcgcg ctccgagccg gtgtaatgca gatggcgctc gatcagctgg tagagccgct
     4921 gctcgtcgaa acgcgtcatg tcggacacat cgacgaggcc atggctttcg aggtcgccgg
     4981 tctgatggcg gctccgctcc atgacgttgt tctcgctcgg caccggctcg agctcgacca
     5041 tggcgagatt gcagcggcgc tcgaagtcgc cggcctcgtc aagcacatag gcgatgccgc
     5101 cagacatgcc tgcagcgaag ttccgcccgg tcgggccgat caccaccacc acgccgccgg
     5161 tcatatattc gcagccatgg tcgccggtgc cttccaccac ggcggtggcg ccggaattgc
     5221 gcacggcaaa gcgctcgccg ccgatgccgc ggaaatagca ctcgccggcg atcgcgccgt
     5281 agagcacggt attgccgacg atgatcgact cctcgggcac gatgccggaa tc
//
LOCUS       DATNAE010000070         3239 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2182016, whole genome shotgun sequence.
ACCESSION   DATNAE010000070 DATNAE010000000
VERSION     DATNAE010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2182016"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="VN518_01365"
     CDS             <1..493
                     /locus_tag="VN518_01365"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HXE03253.1"
                     /translation="VATDVASHLGPAAINARRSIEVIAPDEPVRIRGARDYLFRALRN
                     IVENALRHTPQGTTVSIVVADDPPALKVIDHGPGVRPDQRNAIFRRFWQGGRDQKGGA
                     GLGLDIAARTVVAHGGAISVGEAPEGGAVFTMEFQTISEGMSEVTLPFREPHPAPQPL
                     QRL"
     gene            569..1141
                     /locus_tag="VN518_01370"
     CDS             569..1141
                     /locus_tag="VN518_01370"
                     /inference="COORDINATES: protein motif:HMM:NF023682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermostable hemolysin"
                     /protein_id="HXE03254.1"
                     /translation="MRAIIIPRDDGLRPKAEQLITEVYAQHYGARITTFPDTLVARIA
                     ADNTIECAAGLRFAADGFFSEAYLDAPIDMLLSAIRYIPVRREKIFEVTSLASRAPHL
                     VGSFLRKIIACGEAAGFEWAFFTATLPLKALLERLDLPLVPLADAERSRVANPDSWGT
                     YYELAPRVYAVRRDDIAACLGQNARATVHG"
     gene            1134..2597
                     /locus_tag="VN518_01375"
     CDS             1134..2597
                     /locus_tag="VN518_01375"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXE03255.1"
                     /translation="MVELLDVLREPSRSACGRVAMSDDAGAITGQELIARIAGLAEEF
                     SELPPVLGLIGANGVDWAAAALAAWMAGKTVVPMPTFFSRLQLEHVLRDAGVIHVIAT
                     KEAAETAAGLGVGITAISQRRGETASQAPGAGMIVYTSGSTGRPKGVRLRIEQIDWQA
                     RALGEAIGACPTDLNLSLLPFALLLETITAICVPVLIGARTHFASAVAENVGAGRPAD
                     LLATFEAVQPTTAVLVPQLLSGLVAQLQARKGRAPASLRFVAVGGARVSETLTARAWE
                     LGIPVHEGYGLTECCSVVAVNRPGRRKAGTVGEPLSGLDVRIEDGEIVVSGPAVMEGY
                     LHEPRVGAPWRTGDLGHRDDNGLIAVTGRKDNLLVTAAGRNISPEWIEAMVMSDARVA
                     ACVLLGHGMSHLSVLLVPSLHGEEWLMESPRAHILLWLERLCIDAPSYAVPKDFVVCP
                     MAQAKRIGLLTSNGRVVREAALGIYPALKLARRPIAA"
     gene            2624..>3239
                     /locus_tag="VN518_01380"
     CDS             2624..>3239
                     /locus_tag="VN518_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HXE03256.1"
                     /translation="MEGQLSFYDRLIAETERERIVFTSIPLIREAVRSGASRELYIDF
                     LTQAYHHVKHTFPLLAFAAARTEDETYQDALFEYMEEERGHEKWILNDIAAMGGDAAA
                     VRDGAPGIACQVMVGYTYYAIEWISPYAMLGSVHVLEGMSTLLADKAADAIQRSLGGS
                     GKDGFSYLRSHGALDIEHVAFFKQLANGITDPKTQRIVIDASKVF"
BASE COUNT          537 a         1045 c         1028 g          629 t
ORIGIN      
        1 ggtcgcgacc gacgttgcct cgcatctcgg acccgccgcg atcaatgcgc ggcggtcgat
       61 cgaggtaatc gcgcccgatg agcccgtccg tattcgtggc gcgcgggatt atctcttccg
      121 ggcgctgcgc aatatcgtgg aaaacgctct tcgtcacacg ccgcaaggga ccacggtgtc
      181 gatcgtggtg gctgacgatc cgcctgcact caaggtgatc gatcacggac ccggcgtgcg
      241 ccccgatcaa aggaatgcca tcttccgccg cttctggcag ggagggcgcg atcagaaggg
      301 cggcgccggc cttggactcg atatcgcggc gcgcacggtc gttgctcatg gcggggccat
      361 ttcggtgggc gaggcgccgg agggcggcgc cgttttcacc atggaattcc agaccatttc
      421 ggaggggatg tccgaggtca ccctgccctt ccgcgagccc cacccggcgc cgcaacccct
      481 tcaacgattg tgatcttttg gcggctgtaa ggcgtctgta agcggctcag ccgaccttac
      541 gctggtaaaa ttacggggga attcgttgat gcgggcaata ataattccgc gtgacgatgg
      601 cttgcggcca aaggcagagc aactcatcac ggaggtctat gcgcagcatt atggggcgcg
      661 catcacgacc tttcccgaca cactggtggc gaggatcgcc gccgataaca ccatcgaatg
      721 cgcggccggc ttgcgcttcg ccgctgacgg cttcttctcg gaggcctatc tcgatgctcc
      781 gatcgatatg ctgctgtcag cgatccgtta tattcccgtt cgccgcgaga agatcttcga
      841 ggtcacctcg ctggcgagcc gggcgccgca tttggtcggc tcgttcctgc gcaagatcat
      901 agcctgcggt gaggcggcgg gcttcgagtg ggccttcttc accgccacgc ttcccctcaa
      961 ggccctgctt gagcggctgg acttgcctct cgtgccgctg gccgacgccg agcgctcgcg
     1021 ggtggccaat cccgactcgt ggggaaccta ctacgagctt gccccgcggg tttatgccgt
     1081 tcgccgcgac gacatcgcgg cgtgcctcgg ccagaacgcc agggcgacgg ttcatggttg
     1141 agcttctcga tgtcttgcgt gagccgtcgc gctccgcctg cgggcgcgtg gcgatgtcgg
     1201 acgacgccgg cgcgatcacc ggtcaagagc tcatcgctcg tatcgccggc ctcgccgagg
     1261 agttcagtga gcttccgccg gtgctcggtc ttatcggcgc caacggtgtc gactgggcgg
     1321 ccgcggcact cgcggcgtgg atggccggta agaccgtcgt gccgatgccg actttcttca
     1381 gccgccttca actcgagcat gtgcttcgcg acgcgggcgt catccacgtg attgcgacca
     1441 aggaagctgc tgagacggcg gcgggccttg gcgtcgggat cacggcaatc tctcaacggc
     1501 gaggcgagac ggcatctcag gcgccgggtg ccggcatgat cgtctatacg tcgggtagca
     1561 ccggccggcc aaagggcgtg cggctcagga tcgaacagat cgactggcag gctcgcgctc
     1621 ttggcgaagc gatcggcgct tgtccgaccg atctcaacct atcgctcctg ccgtttgctt
     1681 tactgctcga gaccatcacc gccatctgcg ttccggtctt gatcggcgcg cgcacgcatt
     1741 tcgcttcagc ggtcgccgaa aatgtcgggg ccggtaggcc agccgacttg ctcgcgacct
     1801 tcgaggccgt gcaaccgacc acggccgttc tcgtcccgca attgctgtcc ggactcgttg
     1861 cgcagctcca agcgcgaaaa ggtcgcgcgc cggcgagctt gcgcttcgtc gccgtcggcg
     1921 gcgcgcgcgt ctcggagact ctgaccgcgc gtgcttggga gctcggcatc cctgtgcatg
     1981 aaggctatgg cctcaccgaa tgctgctcag tggtcgccgt gaacaggcca ggtcgccgca
     2041 aggcgggaac cgtgggcgag cccctcagcg gcctcgatgt acgcatcgaa gacggcgaga
     2101 tcgtcgtcag cgggcctgcg gtcatggagg gctatcttca cgagccacgc gtcggagcgc
     2161 cgtggcggac cggcgatctc ggccatcgcg acgataacgg cctcatcgcg gtcacgggcc
     2221 ggaaagacaa tttgctcgtc acagcggcgg ggcggaacat ctcgcccgaa tggatcgagg
     2281 ccatggtgat gagcgatgca cgcgttgccg cctgcgtgct gctcggccat ggcatgagtc
     2341 atttaagcgt gctgcttgtt ccctcccttc acggcgaaga gtggctgatg gaatcgccac
     2401 gcgcccacat tctgctctgg ctcgagagac tctgcatcga cgcgccttcc tacgccgtgc
     2461 ccaaagactt cgtcgtctgt ccaatggcgc aggcaaagcg catcggcctt ctcacctcga
     2521 atggccgcgt cgtgcgcgag gccgctcttg gcatctatcc cgcgctcaag ctcgcgaggc
     2581 gtcccatcgc cgcgtgagat gccgcgccgt cattccacaa gcaatggagg gtcaattgtc
     2641 gttctacgat cgactcatcg ccgaaactga gagggagcga atcgttttca cgtcgattcc
     2701 gctgatccgc gaggcggtgc gctcaggcgc gtcacgcgag ctttatatcg actttctcac
     2761 tcaggcctat caccacgtga agcacacctt cccgctgctt gctttcgcgg cggcgcgcac
     2821 cgaggacgag acctatcagg acgctctctt cgaatatatg gaagaggagc gcggccacga
     2881 gaaatggatc ctgaacgaca tcgcggccat gggcggcgat gccgccgccg tcagagacgg
     2941 tgcacccggc atcgcttgcc aggtcatggt gggctacact tattacgcca tcgaatggat
     3001 aagtccctat gccatgctcg gcagcgtgca tgtgctcgaa ggcatgtcga cgcttctcgc
     3061 cgacaaggcg gctgacgcga tccagcgctc gctcggcggc agcggcaagg acggcttctc
     3121 ctatctccgc tcgcatggcg cgctcgatat cgagcatgtc gccttcttca agcagctcgc
     3181 gaacggcatc accgatccta agacgcaacg gatcgtgatc gacgcctcga aggttttct
//
LOCUS       DATNAE010000071        17677 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3688178, whole genome shotgun sequence.
ACCESSION   DATNAE010000071 DATNAE010000000
VERSION     DATNAE010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 17677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17677
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3688178"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VN518_01385"
     CDS             complement(<1..306)
                     /locus_tag="VN518_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03257.1"
                     /translation="MSITRVAGADRGAGFVATIRLVLPDLLFVLLAVYNAIRLFRHAM
                     WRDELQAFMVAAASNTPLDLFAKLKYEGHPGLWHLLLWVVTRFTTDPVAMQALHLVIA
                     "
     gene            548..1108
                     /locus_tag="VN518_01390"
     CDS             548..1108
                     /locus_tag="VN518_01390"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HXE03258.1"
                     /translation="MAGEVDTPSGVAGRYATALFELALEEKALETVEQDLNRFASALD
                     AVEDLRRLVRSPVFTAEEQQRAIAAILDKMEIEGLTANFLKLIARNRRLYAAPDMIKA
                     FRALLARHRGQASAEVTSALLLTEGQLRALQTALKAALHKDVQLDQKVDATLLGGLVV
                     KVGSRMVDSSLRTKLNSLKQTMKEVG"
     gene            1108..2637
                     /gene="atpA"
                     /locus_tag="VN518_01395"
     CDS             1108..2637
                     /gene="atpA"
                     /locus_tag="VN518_01395"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006013771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HXE03259.1"
                     /translation="MDIRAAEISAILKEQIKNFGQEAEVSEIGQVLSVGDGIARVYGL
                     DNCQAGEMVEFADGTRGMALNLEVDNVGIVIFGDDRHIKEGDVVKRTGAIVEVPVGKA
                     LLGRVVDALGNPIDGKGPIDAQEKRRVDVKAPGIIPRQSVHEPMQTGLKAIDALIPIG
                     RGQRELIIGDRQTGKTAIILDTILNQKSINARGDEKTKLYCVYVAIGQKRSTVAQFVK
                     VLEDNGAMDYSVVVAATASDPAPMQYLAPFAGCAMGEFFRDNGMAAVIFYDDLSKQAV
                     AYRQMSLLLRRPPGREAYPGDVFYLHSRLLERAAKLNKDNGSGSLTALPVIETQANDV
                     SAYIPTNVISITDGQIFLETDLFYQGIRPAVNVGLSVSRVGSAAQVKAMKQVAGKIKG
                     ELAQYREMAAFAQFGSDLDATTQRLLNRGARLTELLKQPQFSPMKVEEQVVVIYAGVN
                     GYLDPIAVKDVQRFEAELLRFTRAEHQDILDDIRDKKEISKETGDKLKAAVDKFAKAF
                     A"
     gene            2657..3544
                     /locus_tag="VN518_01400"
     CDS             2657..3544
                     /locus_tag="VN518_01400"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012336266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HXE03260.1"
                     /translation="MPSLKDLRNRIASVKATQKITKAMQMVAAAKLRRAQSAAEAARP
                     YATRMEAVLANLAAALQGREGGSPLMVGTGKDKVHLLVACTAERGLCGAFNSSIVRLT
                     RDHAFRLMNDGKEVKILCVGKKGFDQLKRLFASRIVEVIELRGVRQLGFNHAERIAEN
                     VLARFAAGEFDVATLFFSRFRSVISQIPTALRIIPAEIPKEATSAQASLGGAVYEYEP
                     DEADILADLLPLNIATQILRALLENAASEQGARMSAMDNATRNAGDMIGRLTLRYNRT
                     RQAMITKELIEIISGAEAL"
     gene            3651..5063
                     /gene="atpD"
                     /locus_tag="VN518_01405"
                     /pseudo
     CDS             3651..5063
                     /gene="atpD"
                     /locus_tag="VN518_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009450696.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
     gene            5066..5470
                     /locus_tag="VN518_01410"
     CDS             5066..5470
                     /locus_tag="VN518_01410"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HXE03261.1"
                     /translation="MADTFKFELVSPERLLVSGEVEQVLVPGSEGDFTMLAHHAPLLT
                     SLRPGLLEIVSSGAERRRYFVRGGFAEVGPQGLTVLAETAIDLVELEAETLNQAIKDA
                     EEDIADAKDEGVRDRAKTQLDHLRQVQTTLSL"
     gene            complement(5483..5989)
                     /locus_tag="VN518_01415"
     CDS             complement(5483..5989)
                     /locus_tag="VN518_01415"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HXE03262.1"
                     /translation="MTLAADLPYRLCVGVMLINRDGYVFVGKRADLPFAPEGTGHWWQ
                     MPQGGLDEGEDPEAAALRELEEETGVTSASILGRTRQWYRYDLPQGLVVTAWHGRYRG
                     QTQLWFAARFEGEDSEIDLEPKPGHEREFDAWRWAPHRELADLVVPFKRPVYEGVIRE
                     FEHFVLGT"
     gene            complement(5998..7215)
                     /locus_tag="VN518_01420"
     CDS             complement(5998..7215)
                     /locus_tag="VN518_01420"
                     /inference="COORDINATES: protein motif:HMM:NF016623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HXE03263.1"
                     /translation="MKALTVTASILFAVLLLGIGWLAFFPGDAGNPVVTVAVAPPPPK
                     PPAPPPSAQPAAPGSSIDLPPGFGIAPVKPQSAPGGAPASQQQQAANQPQAGSPAPGQ
                     PATVQTVAIPAPPGPPPALPPPPEDETASITLVQVPVPELVEESQYGPLPKVATDGRR
                     PIDVYARPSKYAEKAAPGEKPRIAVLINGMGLSDGVTAEAIKGLPAPISVAYGAYGRN
                     LQDWVERARGAGHEVLLQVPLEPLDYPTNDPGPHTLLTSLPPEENMKRLQWLMSRFTG
                     YVGVTNQMGAKFETTQASLTPMLEEIKARGLLFVDDGTVKDSVGSQVAGTIGLDYAAA
                     DVQIDAVPSPDDINKALARLEAVAKEKGSAIGVASAKPGTIKQISQWAGQLQGKGIVL
                     IPVSAAIRSQRQS"
     gene            complement(7285..8628)
                     /locus_tag="VN518_01425"
     CDS             complement(7285..8628)
                     /locus_tag="VN518_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HXE03264.1"
                     /translation="MRKSEYALWTLLVLAVLAAGSTMVGLARSETASAANSEIYRQLD
                     LFGEVLERVRADYVEKPEDAKLIESAINGMLMALDPHSAYLNPKHFRDMQVQTRGEFG
                     GLGIEVTMENGVVKVVAPIEDTPASKAGIQSGDLITALDKEQIQGLTLQEAVEKMRGP
                     VNSAITLTVVRKGVEDPFDVKVVRDVIHINPVKYSVENDVGYIRVTTFNEQTTANLQK
                     AIEDLKKEIGPKLKGFVIDLRNNPGGLLDQAISVSDTFLDQGAIVLTKGRNLEETQRS
                     NARPGDLTDGQKMVVLINGGSASASEIVAGALQDHHRATILGTRSFGKGSVQTIIPLG
                     SNGALRLTTARYYTPSGRSIQAKGIEPEVVVEEELPPDLKAKADQQSTRGEANLRGHL
                     KGEDEDEGGKDEEESGSSSYVAPEKDKDTQLQYAIEILRGTKTVTTDTKKKAEAN"
     gene            complement(8628..9908)
                     /locus_tag="VN518_01430"
     CDS             complement(8628..9908)
                     /locus_tag="VN518_01430"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HXE03265.1"
                     /translation="MSVAAEDDLSREEAQKKLDETSQKLQSTRAQEQGLSQDVAALAE
                     ARAKLNSDLIEAGKQVQASEAKLSGTEAKLAELTDQVTVIRNSITDRKETIVKMLSAM
                     QRIGRTPPPALVTRRDDALAVVRSAMLLAQVFPELKYQADNLSQELDGLVKLETGIRE
                     QRDAEKSEADRLAAERGRIDKLLEEKKVKLAQSEAELSATRQAADQQAAEETELNALI
                     ARLDERIAKAEVAQYDAEVATEKALRAREQQQALATPGNEKVIEIKPESTKIAFASPA
                     RLKPAMPFELTKGTLPLPAQGKRLKRFGEADGVGGTLRGISLQTRGEARIIAPTDGWV
                     VYAGPFRSYGNLLIINAGGGYHVLLAGMGRINVSLGQFVLAGEPVAVMGAALQAGQGN
                     NDNSRPVLYVEFRKDGRPIDPGPWWAEASEKVQG"
     gene            complement(10154..10636)
                     /gene="rlmH"
                     /locus_tag="VN518_01435"
     CDS             complement(10154..10636)
                     /gene="rlmH"
                     /locus_tag="VN518_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011155735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HXE03266.1"
                     /translation="MRLLIAAVGRLKQGPERDLFRHYLGRTEMAGNKLGLAPVTVVEV
                     PESRGPTPAARTAAEAMVMLGKVPPSHKLVCLDREGDQLGSAEFAHAISGFRDRGAPG
                     LAFLVGGAEGLAPEVLARADLKLSLGPLTLPHGLARIVLAEQLYRAATILAGHPYHRA
                     "
     gene            complement(10683..11003)
                     /gene="rsfS"
                     /locus_tag="VN518_01440"
     CDS             complement(10683..11003)
                     /gene="rsfS"
                     /locus_tag="VN518_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HXE03267.1"
                     /translation="MLDTLDNAKAEDVVTIDIKGKTSIGDQMVVASGRSQRHVGAVAD
                     HLIRKLKDEGFGRARVEGLHHCDWVLIDAGDVIVHIFRPEVREFYNLEKMWSADRPME
                     RMVV"
     gene            complement(11229..12017)
                     /locus_tag="VN518_01445"
     CDS             complement(11229..12017)
                     /locus_tag="VN518_01445"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HXE03268.1"
                     /translation="MRKGDSKRPAEHLKVPMAAPNMRIGLLGGSFNPAHAAHRYISLA
                     ALKRVGLDQVWWLVSPGNPLKDASKMPDLEARIETAREVARHPKIVVTGFEAGRPSAY
                     TVDTIGFLKRRYPSVDFVWLMGADNLASFHRWREWEKLFGLVPIAVLDRPGYRLKARA
                     SRAAQRFAFAALDESDARGLASMGPPAWTLLSLPLSRLSSTRLRGGMEGEPPRGKTLR
                     AAKAVKKLAKKAKETAKQEKKQHARQAKKDKKDTKKSKKAAKRH"
     gene            complement(12191..13477)
                     /locus_tag="VN518_01450"
     CDS             complement(12191..13477)
                     /locus_tag="VN518_01450"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HXE03269.1"
                     /translation="MSVLAGQPQASIEPMMLYMGRLAREAAGELALATTAQKNAALEA
                     MAKRLEARTLAILEANKNDIDAARGEGRDEAFIDRMALNEARVAAMAKGLRDIAALPD
                     PVGEVIASWTRPNDLLISRVRVPLGVIGIIYESRPNVTADAGGLCLKSGNAVILRSGS
                     ESFLTSLVICNALTEGLAEVGLPEAAIQLVPTVQREAVGFMLAGLGGAIDVIVPRGGK
                     SLVARVQEEARVPVFAHLEGVCHVYVHQPADPAMAKIIVLNAKMRRTGVCGAAETLLV
                     DRAAAPTHLAPLVKMLIEADCEVRGDADTQKADARVTPATEEDWAKEYLDAIIAVKVV
                     DGLDEAIDHIGRYGSQHTEAIITEDKAAAEKFLTRVDSAIVLHNASTQFADGGEFGMG
                     AEIGIATGRFHARGPVGLEQLTTFKYVVHGSGQIRP"
     gene            complement(13487..14611)
                     /gene="proB"
                     /locus_tag="VN518_01455"
     CDS             complement(13487..14611)
                     /gene="proB"
                     /locus_tag="VN518_01455"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HXE03270.1"
                     /translation="MRAEWASAARLVVKIGSSLLVDSGTGALKAAWLGSLCDDIAKLK
                     AQGKEIIVVSSGAIALGRNVLKLPKGPLKLEDSQAAAAVGQIDLAHAYEDAFRARGIV
                     AAQVLLTLGDTEERRRYLNARNTVTTLLKLGAVPVVNENDTVATSEIRYGDNDRLAAR
                     VASMMSADCLVLLSDVDGFYTAPPGSAPDARRLDEVRDITPEIEAMAGDVGSELSRGG
                     MVTKIEAAKIAVAGGTHMAIASGKVLNPLEALAGPGGCTWFLAHSDPVAARKRWIAGT
                     LEPKGAMIVDRGAAEALTRGKSLLPAGVTKIEGSFERGDAVVIRNGEGAELGRGLAAY
                     DHEDARRIIGRKSSELAEILGYRGDPELIHRDDMVLHRRS"
     gene            complement(14608..15645)
                     /gene="obgE"
                     /locus_tag="VN518_01460"
     CDS             complement(14608..15645)
                     /gene="obgE"
                     /locus_tag="VN518_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HXE03271.1"
                     /translation="MQFLDQARVYVRSGDGGNGCVGFRREKFIEFGGPDGGDGGKGGD
                     VILECVANLNTLLDYRYQQHFKAKKGGNGMGQNRTGAGGADVVLKLPPGTQVFEEDGE
                     TLIADLVNPGDRVVLLRGGNGGFGNTRFKSSTNQAPRRANPGQPGKEMTIWLRLKLIA
                     DAGLVGLPNAGKSTFLAAVSAAKPKIADYPFTTLHPNLGVVRVDSTDFVLADIPGLIE
                     GAHEGAGIGDRFLGHVERCAVLLHLVDATLDDPAEAYRIIRGELEAYGAGLEDKAEIV
                     ALSKCDAVPDDELKRKAKSLAKAAGRKPLVLSAVSGTGMKEALRELAAIVKAARAKRN
                     TPEPREAGWQP"
     gene            complement(15761..16333)
                     /locus_tag="VN518_01465"
     CDS             complement(15761..16333)
                     /locus_tag="VN518_01465"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXE03272.1"
                     /translation="MAIRTERLLLRTPVLADAASISLLAGDYEVASMTGTIPHPYSEE
                     MAVDWIQSLQAGEEGVAFTIDRGGALIGCVGYRATEKTHAEMGYWIGKPYWGMGYATE
                     AARALILHAFHKERFDYLTVGHFKENPASARVIAKLGFEPSGEVLRDCAARNNKARCL
                     TYRLTRERGLASLRPGQEASPLSAAGRGLG"
     gene            complement(16404..16658)
                     /gene="rpmA"
                     /locus_tag="VN518_01470"
     CDS             complement(16404..16658)
                     /gene="rpmA"
                     /locus_tag="VN518_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012172613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HXE03273.1"
                     /translation="MAHKKAGGSSRNGRDSAGRRLGVKKSGGQRVIPGNIIVRQRGTK
                     WHPDDGVGMGVDHTLFALREGDVEFATKAKGRVYVSVKPN"
     gene            complement(16670..17224)
                     /gene="rplU"
                     /locus_tag="VN518_01475"
     CDS             complement(16670..17224)
                     /gene="rplU"
                     /locus_tag="VN518_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HXE03274.1"
                     /translation="MFAVIRTGGKQYRVAPNDIIEIERVAGEPGDIITLGEVLLLGGD
                     SPKTGSPTIAGALVAAEVMEQSRGDKIIVFKKKRRKNYRRKKGHRQDLTLLRITEILT
                     DGKKPSQTKVARPEPKKVEAKKPEADEAKAEAKPAKKPAAKKAAPEKKAAPAKKPAAK
                     AAAAKKPAAKAAAKKPAAKPAKKK"
     gene            17477..17566
                     /locus_tag="VN518_01480"
     tRNA            17477..17566
                     /locus_tag="VN518_01480"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17511..17513,aa:Ser,seq:cga)
BASE COUNT         3018 a         5842 c         5543 g         3274 t
ORIGIN      
        1 cgcgatgaca agatgcaacg cctgcatcgc caccggatcg gtggtgaagc gcgtgaccac
       61 ccacaacagc aggtgccaga gcccgggatg gccctcatat ttgagcttgg cgaagagatc
      121 gagcggcgtg ttgctggccg cagccaccat gaaggcctgc agctcgtcgc gccacatggc
      181 atggcggaac aacctgatcg cgttgtagac ggcgaggagg acaaacagca gatctgggag
      241 caccaagcgg atcgtggcga cgaagcccgc gcccctatcg gcacccgcga cgcgcgtgat
      301 cgacatccgc cctcaccccc gctccttgcc catcccaatc ggccagtgcc tgcaaggccc
      361 aggcgatctg gcctcgcctt attgcaccgc cgcaaccctt atgttacagc ctcggcgggc
      421 cttccggtgg gccgtcggcg gctcattttc aggctgttga cgcggaggct ccaatcccag
      481 aatttgctgg tccagggacg agaccgcgct tgaaaagcgc ccctcccggg aggaaaggcg
      541 ttagttcgtg gcaggtgaag tagacacccc ttcaggcgtc gcgggccgct atgccaccgc
      601 cctgttcgag cttgcgctgg aagagaaggc gcttgagacg gtcgagcagg atttgaaccg
      661 cttcgcctct gcgcttgacg ccgtcgagga tttgcgccgc ctggtgagga gcccggtatt
      721 caccgccgag gagcagcagc gcgccatcgc tgccatcctc gacaaaatgg aaatcgaagg
      781 ccttaccgcg aacttcctca agctcatcgc caggaaccgc cgcctgtacg ccgcccccga
      841 tatgatcaag gcgtttcgcg cgctgctcgc gcgccatcgc ggccaggcat ccgccgaggt
      901 cacttcggcg ctcctcctca ccgaaggcca actgcgagcg ttgcagaccg cgctcaaggc
      961 cgcactgcat aaggacgtgc agctcgatca gaaggttgat gccaccctcc tcggcggtct
     1021 cgtcgtcaag gtcggcagcc gcatggtcga ctcgtcgctg cgcaccaagc tcaacagtct
     1081 caagcaaacc atgaaagagg ttggctgatg gatatcaggg ccgccgagat ctccgcgatc
     1141 ctcaaggagc agatcaagaa ttttggccag gaggcggaag tctccgagat cggccaggtg
     1201 ctgtcggtcg gtgacggcat cgcccgcgtc tatggcctcg acaattgcca ggccggcgag
     1261 atggtcgagt ttgccgacgg cacgcgcggc atggcgctga acctcgaggt cgacaatgtc
     1321 ggcatcgtga tcttcggcga cgaccgccac atcaaggaag gcgacgtggt caagcgcacc
     1381 ggcgccatcg tcgaggtgcc ggtcggcaag gcgctcttgg gccgcgtggt ggatgcgctc
     1441 ggcaacccga tcgacggcaa gggcccgatc gatgcgcaag agaagcgccg cgtcgacgtg
     1501 aaggcgcccg gcattattcc ccgccaatcg gtgcacgagc cgatgcagac gggcttgaag
     1561 gccatcgacg cgctcattcc catcggccgc ggccagcgcg agctgatcat aggcgaccgc
     1621 cagaccggca agaccgccat cattctcgac accatcctca atcagaaatc gatcaatgcg
     1681 cggggcgacg agaagaccaa gctctattgc gtctatgtcg ccatcggcca gaagcgctcg
     1741 accgtcgcgc aattcgtcaa ggtgctcgag gacaacggcg ctatggacta ttccgtcgtg
     1801 gtcgctgcca ccgcctctga cccggcgccc atgcagtatc tcgcgccctt cgccggctgc
     1861 gccatgggcg agttcttccg cgacaacggc atggccgcgg tgattttcta cgacgacctg
     1921 tccaagcagg ccgtcgccta ccgccagatg tcgctgctgc tccgccggcc gcctggacgc
     1981 gaggcctatc ccggcgacgt gttctatctg cattcgcgcc tgctcgagcg cgccgccaag
     2041 ctcaacaagg acaacggctc aggctcgctg acggcgctgc cggtgatcga gacgcaggcc
     2101 aacgacgtgt cggcctatat cccgaccaat gtgatctcca tcactgacgg ccagatcttt
     2161 cttgagaccg atttgttcta tcagggcatt cgtcccgcgg tgaatgtcgg cctctcggta
     2221 tcccgtgtgg gctccgccgc ccaagtgaag gcgatgaagc aggtcgccgg caagatcaaa
     2281 ggcgagcttg cccaatatcg cgagatggcg gccttcgctc agttcggctc cgacctcgac
     2341 gccaccaccc agcgcctgct caaccgcggc gctcgcctca ccgagctttt gaagcagccg
     2401 caattctcgc cgatgaaggt cgaggagcag gtcgtggtga tctatgccgg cgtgaacggc
     2461 tatctcgatc cgatcgcggt gaaggacgtg cagcgcttcg aggccgagct actgcgtttc
     2521 acgcgcgccg agcatcagga cattctcgac gacatccgcg acaaaaagga aatctccaag
     2581 gagaccggcg acaagttgaa agccgccgtc gacaaattcg ccaaggcctt cgcgtaagga
     2641 ttagcggtaa cgccccatgc cgtcgctgaa agacctgcgc aatcgcatcg cctcggtgaa
     2701 ggcgacgcaa aagatcacca aggccatgca gatggtggcg gcggcgaagc tcaggcgcgc
     2761 gcagagcgcc gcggaagcgg cgaggcctta cgccacgcgc atggaggccg tgctcgccaa
     2821 cctcgccgcg gcgctgcaag gccgcgaggg cggctcgccg ctgatggtcg gcaccggcaa
     2881 ggacaaggtc catctcctcg tggcgtgcac cgccgagcgc ggactgtgcg gagccttcaa
     2941 ctcatccatc gtcaggctca cccgcgatca cgcctttcgg ctgatgaacg acggcaagga
     3001 ggtgaagatc ctttgcgtcg gcaagaaggg cttcgaccag ctcaagcgtc tgttcgcctc
     3061 gaggatcgtc gaggtgatcg agctcagagg cgtcaggcag ctcggcttca accacgccga
     3121 gaggatcgcc gagaacgtct tggcgcgctt cgctgccggc gagttcgacg tggcaaccct
     3181 gttcttctcg cgcttccgct cggtgatcag ccagatcccc acggccttgc gcatcatccc
     3241 tgccgagatc ccgaaggaag ccacctcggc gcaagcctcg ctcggcggcg cggtctatga
     3301 atacgagcct gacgaggccg atatcctcgc cgatctcctg ccgctcaaca tcgccacgca
     3361 aatcctccgc gcgttgctcg agaacgcagc gtcggagcag ggcgcgcgca tgagcgccat
     3421 ggacaacgcg acgcgcaatg ccggcgacat gatcgggcgc ctgacgctca gatataatcg
     3481 cacgcgacag gccatgatca ctaaggagct gatcgagatc atttccggcg ccgaggcgct
     3541 ctagtttcga gatgtgagga acgttagaca tggcacgtaa atcccccgtg atcgccgaaa
     3601 ccaagacgca agagaagcgg atcgccgcgg gcaaaggccc gaaaggtcat gtgcgccaag
     3661 tcatgggcgc cgtggtcgat gtgcaattcg gcgagcatct gccggaaatc ctgaacgcgc
     3721 tcgagaccga caatcacggc aaccggctcg tgctcgaagt cgcccagcat cttggcgaga
     3781 gcacggtgcg cactattgcc atggattcca ccgaaggcct ggtgcgcggt caggaagtgg
     3841 tcgacaccgg cgagccgatc gcggttccgg tcggcgacga gaccttgggc cgcatcatga
     3901 acgtggtcgg cgaggcggtc gacgaggccg gcccgatcaa ggccgccgag aagcggccga
     3961 tccacgcgcc ggcaccatct tacgttgatc aatcgaccga agccgaaatt ctcgtcaccg
     4021 gcatcaaggt ggtcgatctg ctggcgcctt acgccaaggg cggcaagatc ggcctgttcg
     4081 gaggtgctgg cgtcggcaag accgtgctga tcatggagct gatcaacaac gtggccaagg
     4141 cccatggcgg ctattcggtg ttcgccggcg tcggcgagcg cacccgcgag ggcaacgacc
     4201 tctatcacga gatgatcgag tcaggcgtga acaaggaccc gcgcaaggac ggcacggcgg
     4261 aaggctcgaa atgcgcgctg gtctacggcc agatgaatga gccgcccggc gcccgcgccc
     4321 gcgtcggcct gtcgggcctc actgtcgccg aatatttccg cgaccagggc caggacgtgc
     4381 tgttcttcgt cgacaacatc ttccgcttca cccaggcagg ctccgaggtg tcggcgctgc
     4441 tcgggcgcat cccctcggcc gtgggctatc agccgacgct cgccaccgac atgggggcgc
     4501 tgcaggagcg catcaccacg acgcagaagg gctcaatcac gagcgtgcag gcgatctatg
     4561 tgcctgccga cgatctgacc gatcctgcgc cggcaacctc cttcgcccat ctcgacgcca
     4621 ccaccgtgct cgcccgcggc atcgccgaga agggcatcta tcccgccgtg gacccgctcg
     4681 attcgacctc gcgcatgctt tcgccgctga tcgtcggcga agagcattac gcggtggcac
     4741 ggcgcgtgca ggagatcctg caacgctaca aggcactgca agacatcatc gccattctcg
     4801 gcatggacga gctgtcggaa gaggacaaga tcgccgtggc gcgcgcccgc aagatcgagc
     4861 gcttcctgtc gcagcccttc ttcgtggccg aagtgttcac cggctcgccg ggcaagttcg
     4921 tcgacctcgc cgacaccatc aagggcttca aaggactgtg tgacggcgag tacgatcatc
     4981 tgccggagca ggccttctac atggtcggca ccatcgagga ggccgtggcc aaggccgaga
     5041 agcttgccgc cgaggccgct tgagcatggc tgacaccttc aaattcgagc tggtctcgcc
     5101 tgagcggctg ctcgtctccg gcgaggtcga gcaggttctg gtgcctgggt ccgagggcga
     5161 cttcaccatg ctcgcccatc acgccccgct gctcacgagt ctccggcctg gccttctcga
     5221 aatcgtctcg agcggcgccg agcgccggcg ctatttcgta cgcggcggct ttgccgaggt
     5281 cgggccgcaa ggcctcaccg tgctcgccga gacggccatc gacctcgtcg agctcgaagc
     5341 tgaaacgctc aaccaagcga tcaaggacgc cgaggaagac atcgccgacg ccaaggacga
     5401 aggggtgcgc gaccgcgcca agacccagct cgaccacctc cgccaggtgc agacgacgct
     5461 cagcctctag ccttgccgca gtttaggtgc cgagcacgaa gtgctcgaat tcccggatca
     5521 cgccctcata gaccgggcgc ttgaacggca cgacgaggtc tgcaagctct cgatgcggcg
     5581 cccatctcca ggcgtcgaac tcgcgctcat ggcctggctt cggctctaga tcgatctcgc
     5641 tgtcctcgcc ttcgaatctg gcggcaaacc agagctgcgt ctggccgcga tagcgcccat
     5701 gccaggcggt gaccaccaac ccttgcggca gatcgtacct gtaccattgc cgcgtccggc
     5761 cgaggatgct ggcgcttgtc accccggtct cctcctcaag ctcgcgcaaa gctgcagctt
     5821 cggggtcttc accctcatcg aggcctcctt gcggcatctg ccaccaatgc ccggtcccct
     5881 ccggagcgaa aggcaaatcg gcgcgcttgc cgacgaacac ataaccgtcg cggttgatga
     5941 gcatcacgcc gacgcaaagg cggtagggca aatctgccgc taaggtcatt ggcaggatca
     6001 gctttgccgc tgcgagcgga tcgccgcgct gaccgggatg aggacgatgc ctttgccttg
     6061 aagctgcccg gcccattggg agatctgctt gatggttccg ggcttggcgc tggcgacgcc
     6121 gattgccgag cccttttcct tggcgacggc ttcgaggcgg gcaagtgcct tgttgatatc
     6181 gtcaggcgat gggacggcat cgatctggac gtcggcggcg gcatagtcga ggccgatggt
     6241 gccggcgact tgggagccga ccgaatcctt caccgttccg tcgtcgacga acagcagccc
     6301 gcgcgccttg atctcctcga gcatcggcgt gagcgaagcc tgcgtcgtct cgaacttcgc
     6361 ccccatctga ttggtgacgc cgacatagcc ggtgaagcgc gacatcagcc attgcagccg
     6421 cttcatgttc tcctctggcg gcagcgacgt cagcagcgtg tgcggtcccg gatcgttggt
     6481 cggatagtcg agtggctcga gcggcacctg caacagaacc tcgtgccccg cgccgcgcgc
     6541 cctctccacc cagtcctgaa gattgcggcc ataggcgcca taggccacgc tgatcggcgc
     6601 gggcaacccc ttgatcgcct cggcggtgac tccgtcagac aagcccatgc cgttgatcag
     6661 caccgcgatg cgcggctttt cgccgggcgc cgccttctcc gcgtattttg acgggcgcgc
     6721 ataaacgtca atgggccgcc ttccgtcggt agctaccttg ggaagcggcc catattgaga
     6781 ctcctcgacc agctcgggta cggggacctg cacgagcgtg atcgaagctg tttcatcttc
     6841 aggtggcggc ggcagagctg gcggcgggcc gggaggcgcc ggaatagcaa cggtttggac
     6901 cgtcgccggc tgacctgggg ccggcgatcc agcttgtggc tggttcgccg cctgctgctg
     6961 ctgagaggct ggagctcctc ctggggctga ctgcggcttc accggagcga tgccgaaacc
     7021 gggcggcaga tcgattgatg accccggagc tgccggctga gcagaaggcg gcggtgcggg
     7081 cggttttggc ggcggcggcg caactgccac ggtgaccacg gggttgccgg catcaccagg
     7141 gaagaaggca agccaaccaa taccgagcaa cagcaccgcg aacaggatcg aagccgtgac
     7201 ggtcagcgcc ttcatctaac cctgtacctc cccgcgacca ggccccccgg tcagggggcc
     7261 gaagcctcct ccatcggagg acgattaatt ggcctcagcc ttcttcttgg tgtccgtggt
     7321 gaccgtcttg gtgccgcgca ggatctcgat cgcgtattgc agctgggtat ccttatcctt
     7381 ctccggcgcg acgtatgacg agctgccgga ctcttcttcg tccttgccgc cttcatcctc
     7441 gtcctcaccc ttgaggtgcc cgcgcaaatt cgcctcgccg cgcgtcgact gctggtcggc
     7501 cttcgccttt aggtcggggg gcaactcctc ctcgaccacc acctcaggct cgatgccctt
     7561 ggcctgaatc gatcggcccg acggcgtgta gtagcgcgcg gtggtgagcc ggagcgcgcc
     7621 gtttgatccg agcggaatga tggtctgcac cgagcccttg ccgaaggacc gcgtgccgag
     7681 gatggtcgcg cgatggtggt cctgcagcgc gccggcaacg atttccgacg ccgaggccga
     7741 gccgccattg atcagcacca ccatcttttg gccgtcggtg aggtctccag gccgcgcatt
     7801 cgaccgctgc gtctcttcga gattgcggcc cttggtcagc actatggcgc cttgatcgag
     7861 gaacgtatcc gacaccgaga tcgcctgatc gagcaggccg cccgggttgt tgcgcaggtc
     7921 gatgacaaag cccttgagct tgggaccgat ctccttcttc agatcctcga tcgccttctg
     7981 cagattggcg gtggtctgct cgttgaaggt ggtgaccctg atatagccga cgtcgttctc
     8041 gacgctgtac ttcaccggat tgatgtggat cacgtcgcgc acgaccttca cgtcgaaggg
     8101 gtcctcgacg cccttgcgca caacggtcag cgtgatcgcg gaattgaccg gcccgcgcat
     8161 cttctccacc gcttcctgca gcgtaaggcc ttggatctgc tccttgtcga gcgcggtgat
     8221 cagatcgccg ctctggatgc ccgccttcga tgccggtgta tcctcgatcg gggcgacgac
     8281 tttgaccacg ccgttctcca tggtcacctc gatgccgagg ccgccgaact cgccgcgcgt
     8341 ctgcacctgc atgtcgcgga agtgtttcgg attgagatag gcggaatgcg gatcgagcgc
     8401 catcagcatg ccgttgatcg ctgattcgat cagcttggcg tcctccggct tctcgacata
     8461 gtcggcgcgc acgcgctcga gcacttcgcc gaacagatcg agctgccggt agatttccga
     8521 gttggcggcc gacgccgtct cgctccgcgc gagccccacc atcgtcgaac cggcagcgag
     8581 cacggccagc accagaagcg tccatagcgc gtattctgat ttccgcatca tccttgcacc
     8641 ttctcggaag cctcggccca ccatgggccg gggtcaattg gcctgccgtc cttcctgaac
     8701 tcgacgtata ggacggggcg cgaattatcg ttgttgcctt ggcctgcctg gagcgccgcg
     8761 cccatcaccg cgaccggctc gccggctaag acaaactggc cgaggcttac gtttatgcgg
     8821 cccattcccg caagcagaac atgatagcct ccgccagcat tgatgatcaa tagattgcca
     8881 taggaccgga agggcccggc atagaccacc cagccgtcgg ttggggcgat tatccgtgct
     8941 tctccacggg tttgcaggga aatgccccgc aacgtgccgc cgacgccgtc ggcctcgccg
     9001 aagcgcttca gccgcttgcc ctgggctggc agaggcaagg tccccttggt cagctcgaac
     9061 ggcattgccg gcttcaagcg ggcagggctg gcaaaggcga tcttggtcga ctcaggcttg
     9121 atctcgatga ccttctcgtt acccggggtg gcgagcgcct gttgctgctc gcgggcccgc
     9181 aacgccttct cagtggccac ttcagcgtcg tattgcgcga cctcggcctt ggcgatccgc
     9241 tcgtcgagcc gggcgatcag cgcgttgagc tcggtctcct cggcagcttg ttggtcggcg
     9301 gcctggcggg tcgctgaaag ctcggcttcg ctctgagcga gcttcacttt cttttcctca
     9361 agcagcttgt cgatgcggcc gcgctcggcg gcaagcctgt cagcctcgct cttctcggca
     9421 tcacgctgct cgcgaatgcc ggtttccaat ttcaccagtc cgtcgagctc ctgcgacaga
     9481 ttgtcggcct gatatttgag ctcgggaaac acctgggcca gcagcatggc gctgcgcacc
     9541 acggcaagtg catcgtcgcg gcgcgtgacc agcgccggcg gcggcgtgcg gccgatcctc
     9601 tgcatggccg agagcatctt gacgatggtt tcctttcgat cggtgatcga gttgcgaatg
     9661 accgtgacct gatcggtaag ctcggcgagc ttggcttcgg tcccggacaa ttttgcttcg
     9721 ctcgcctgca cctgctttcc tgcttcgatc agatcgctat tgagcttggc ccgcgcctcg
     9781 gcgagcgccg cgacatcctg ggaaagaccc tgctcctggg cgcgggtcga ttgcagcttc
     9841 tgcgaggtct cgtcgagttt cttctgtgcc tcctcgcggg agaggtcgtc ctccgccgca
     9901 acagacaacg ggaaagtgct taagagtccc aggagaagaa gcccggttat gacgaaccgg
     9961 aatgtcggct caggggaccg atggattggc gccatccttc gtaaccagcg tgctcttccc
    10021 ccgtcccgtc tcttgcccgc cggcttgaag agttggaact cgagcgagct ggaacggaag
    10081 cggagcgatg aggcagaatc aaggcgaata tgctgaacaa accttaaccc ttccctccaa
    10141 cggggagcgg aggtcaggcg cggtgataag ggtgaccggc aagaatggtc gccgcgcgat
    10201 agagctgctc ggcgagcacg atgcgggcaa gcccatgcgg cagcgtgagg ggaccgagcg
    10261 atagcttgag atcggcgcgg gcgagcacct ctggcgccaa gccctcggcg ccgccgacga
    10321 gaaaagcgag gccgggggcg ccgcggtcgc gaaatccgct gatggcgtgc gcgaactcgg
    10381 cgctcccgag ctgatcgccc tcgcggtcga ggcaaaccaa tttatgcgag ggtggcacct
    10441 tgccgagcat gaccatggct tcggcggcgg tgcgcgcggc cggcgtgggc ccccgcgact
    10501 caggcacctc gaccaccgtc accggcgcaa gcccgagctt gttgccggcc atctcggtgc
    10561 ggccgagata atgcctgaac aggtcacgtt ccgggccttg cttcagcctg cccacggcag
    10621 caatgagaag ccgcatggcg cgaccttgta aggcctaggc cccctggaac gctagcggcg
    10681 gatcagacca ccatgcgctc catcgggcgg tcggcggacc acatcttctc cagattatag
    10741 aactcccgca cctctggacg gaaaatgtgg acgatgacgt cgcccgcgtc gatcagcacc
    10801 cagtcgcaat ggtgaaggcc ttcgacgcga gcgcgtccga agccttcgtc cttgagcttc
    10861 ctgatgaggt gatcggcaac ggcgccgaca tgccgttgcg agcgccccga ggctacgacc
    10921 atctggtcgc cgatcgaggt cttgcccttg atgtcgatgg tgacgacgtc ctcggccttg
    10981 gcattgtcga gtgtgtcgag cacgacgtcg agcaaacagt tcgggtttgc gcgaaatgac
    11041 gcaaagccgg agggaggcgt acccttaaag gtaccctctc ttggtgagcg catgttttgg
    11101 tggatatcct tctgtcattg ctcctgcgga agcggcttct ctcagcctcg tttcggctaa
    11161 acgccgaagc ggctcattcg tgccgcctca cctcgaacat agtctgcgtc gcggcaaaat
    11221 ttcaatagct agtgtcgctt cgccgctttc ttacttttct ttgtgtcctt cttgtccttc
    11281 ttcgcctgcc tggcatgctg tttcttttcc tgcttggccg tctccttcgc cttctttgcc
    11341 agttttttca cggcctttgc cgccctgagc gtcttcccgc gaggcggctc accctccatg
    11401 ccgccgcgga gccttgtgga ggaaagcctc gacagcggca gcgacagcaa ggtccaggcc
    11461 ggcggtccca tgctggcaag tccgcgcgca tcggattcgt cgagcgcggc gaaggcaaag
    11521 cgctgcgcag cccggctcgc gcgagccttc agccggtagc ccggacggtc gagcacggcg
    11581 atcggcacga ggccgaacag cttttcccat tcccgccagc gatggaacga ggcgagattg
    11641 tcggccccca tcagccacac gaaatcgacg gagggatagc gacgcttgag gaagccgatg
    11701 gtatcgaccg tgtaggcgct cgggcggccg gcctcaaagc ccgtgaccac gatctttgga
    11761 tgacgtgcga cctcccgcgc cgtttcgatg cgcgcttcaa ggtcgggcat cttgctcgcg
    11821 tctttgaggg gattgcctgg gctcaccagc caccacacct gatcgagtcc aacccgcttc
    11881 agagccgcga ggctgatata gcggtgtgcc gcgtgcgcgg ggttgaacga gccgccgagc
    11941 agcccgatgc gcatgttcgg cgccgccatc ggcaccttga gatgctcggc ggggcgcttg
    12001 ctgtcgcctt tacgcatgcg gcgtatccgc tcgctccata cctccccctc gcggggaggt
    12061 ccggcgacgc cccttggcgt cgctggggtg ggggctcatt tcagcgtcag gactcgtcat
    12121 tcgcaatcat cacgcccgct cgccaaagcg agcctttggc gaccaccctc cccgtcccgg
    12181 ggagggatgc tcaaggacgg atctggcctg agccgtgcac gacatatttg aaggtcgtga
    12241 gctgctcgag ccccacaggc ccgcgagcat ggaagcgccc ggtggcgatg ccgatctcgg
    12301 ctcccatgcc gaactcgccg ccgtcggcga attgcgtcga ggcattgtgc agcacgatgg
    12361 cactgtccac gcgggtgaga aacttctcgg ctgccgcctt atcctcggtg atgatggcct
    12421 cggtgtgctg cgagccgtag cggccgatat ggtcgatggc ttcgtcgagc ccgtcgacca
    12481 ccttcaccgc gatgatggcg tcgagatatt ccttcgccca atcctcttcg gtggctggcg
    12541 tgacgcgcgc atcggctttt tgcgtgtcgg catcgcccct cacctcgcaa tcggcctcga
    12601 tgagcatctt gaccagaggc gcaagatggg tcggcgctgc ggcgcgatcg acgagcaaag
    12661 tctccgccgc gccgcacacc ccggtccgcc gcatcttggc gttgagcacg atgatcttgg
    12721 ccatggcggg gtccgcaggc tgatgcacgt agacgtggca gacgccttcg agatgcgcga
    12781 acaccggcac ccgcgcctct tcctgcacgc gggcgaccag gctcttgccg ccgcgcggca
    12841 cgatcacgtc gatggcgccg ccaagccctg cgagcatgaa gccaaccgcc tcacgctgca
    12901 ccgtgggcac gagctggatc gctgcttccg gcaaccctac ctcggcaagg ccctcggtca
    12961 gtgcgttgca gatcacgagc gaggtgagga agctctccga gcccgagcgc aggatcacgg
    13021 cgttgcctga tttgaggcaa agcccgccgg cgtcggccgt cacgttcggc cgcgactcat
    13081 agatgatgcc gatcacgcca agcggcacgc gcacgcgtga aatcagcaag tcgttcggcc
    13141 gcgtccacga tgcgatcacc tcgccgaccg gatccggcaa cgcggcgatg tcgcgcaagc
    13201 ctttcgccat cgccgccacg cgcgcttcgt tcagcgccat ccggtcgatg aaggcctcgt
    13261 cgcggccctc gccgcgcgcg gcgtcgatat cgtttttgtt ggcttcgagg atcgcgagcg
    13321 tacgcgcctc gagccgcttg gccatggcct cgagcgcagc gttcttctgc gccgtcgtgg
    13381 caagcgccag ctcgcccgcg gcctcgcgcg ccaagcgtcc catatagagc atcatcggct
    13441 cgatgcttgc ttgcggttgt ccggccagaa ccgacatcac gtttcctcaa ctccttcggt
    13501 gcagcaccat atcgtcgcga tggatcaact ccgggtcgcc acggtagccg agaatctccg
    13561 ccaattcact gctcttgcgc ccgatgatgc ggcgcgcgtc ctcatggtcg taggcggcaa
    13621 gaccgcgtcc cagctcggcg ccctcgccgt tgcggatcac cacagcgtcg ccgcgctcga
    13681 agcttccttc tatcttggtg acgccggctg gcaacaggct cttgccgcgc gtgagggcct
    13741 cggcggcgcc cctgtcaacg atcatcgcgc cctttggctc cagcgtgccc gcgatccagc
    13801 gcttgcgcgc ggccacggga tcggaatgcg ccaggaacca ggtgcagccg ccggggccgg
    13861 caagcgcctc aagcggattg agcaccttgc cgctcgcgat cgccatatgc gtgccgccgg
    13921 caacggcgat cttcgccgcc tcgatcttgg tcaccatgcc gccgcgcgac agctcggagc
    13981 cgacgtctcc ggccatggcc tcgatctcag gcgtgatgtc acggacctcg tccagccgcc
    14041 ttgcgtcggg cgccgaccca gggggcgccg tatagaaacc gtcgacgtcg gacagcaaga
    14101 ccaggcagtc ggcgctcatc atcgaggcga cccgcgccgc gagccggtcg ttgtcgccgt
    14161 agcggatctc gctggtcgcg accgtgtcgt tctcgttgac caccggcacg gcgccgagct
    14221 tgagcagcgt ggtcacggtg ttgcgcgcgt tgagatagcg ccgccgttcc tcggtatcgc
    14281 cgagggtgag caggacttgc gccgcaacaa tgccacgggc gcggaaggca tcctcataag
    14341 catgcgcgag atcgatctgc ccaacggcgg cagcggcctg gctgtcttcg agcttgagcg
    14401 gacccttcgg cagcttcagc acgttccggc cgagcgcgat ggcgcctgac gacacgacga
    14461 tgatctcctt gccttgcgcc ttgagcttgg cgatgtcgtc gcagagcgag ccgagccacg
    14521 ccgctttcaa cgcgccggtc ccgctatcca cgagaagcga ggagccgatc ttgaccacga
    14581 gccgagcggc gcttgcccat tccgccctca tggctgccac ccggcttcgc gcggctcagg
    14641 cgtgttgcgc ttggcgcgcg ccgccttgac gatcgcggca agctcgcgca acgcctcctt
    14701 catgccggtg ccgctcaccg cggacaggac gagcggcttt cgtcccgcag ccttggccag
    14761 cgacttcgcc ttgcgcttga gctcatcgtc aggcaccgca tcgcacttcg acagcgcaac
    14821 gatctccgcc ttgtcctcta gccccgcgcc ataggcttcg agctcgccgc ggatgatgcg
    14881 ataggcctcg gcgggatcgt caagcgttgc atcgacgaga tgtaggagca cggcgcagcg
    14941 ttcgacgtga ccgagaaagc gatcgcctat gcccgcccct tcatgcgcgc cttcgatcag
    15001 gcccggaatg tcggcgagca cgaagtcggt gctgtcgact cgcaccacgc cgagattggg
    15061 atggagcgtg gtgaagggat agtcggcgat cttcggtttc gccgcgctca ctgcggcgag
    15121 gaaggttgac ttgccggcat tgggcagccc gacaagcccg gcatcggcga tcagcttcag
    15181 ccggagccag atggtcatct ccttgccggg ctgtcccgga ttggcccggc gcggcgcctg
    15241 attggtggag gacttgaagc gcgtgttgcc gaagccgccg ttgccgccgc ggagcagcac
    15301 cacacggtcg ccggggttga cgaggtcggc gatcagcgtc tccccgtcct cctcgaaaac
    15361 ttgcgtgccg ggcggcaatt tcagcacgac gtcggcgccg cccgcgccgg tgcggttctg
    15421 gcccatgccg ttgccgccct tcttcgcctt gaagtgctgc tggtagcgat agtcgagcag
    15481 cgtgttgagg ttcgccacac attcgaggat gacgtcgccg cccttgccgc cgtcgccgcc
    15541 gtcaggaccg ccgaactcga tgaacttctc gcggcggaag ccgacgcagc cattgccgcc
    15601 gtcgccggag cgcacataaa ccctggcttg atcgaggaac tgcatgggac gcgggtcaca
    15661 ggagtaaggg ccacctctcc ccttgggcga gaggtcgcgc cgagctggct cggcgggtca
    15721 agggtgttaa agcacaggtt gtttaactta aaactccccc tcaccccaac cctctccccg
    15781 ctgcggaaag gggggacgct tcctgcccag ggcgtaaaga ggccagcccc cgctcgcggg
    15841 tgagccggta ggtgagacag cgcgccttgt tgttccgggc ggcgcagtcg cgcaatacct
    15901 cgccggaagg ctcgaagccg agcttggcga tcacccgcgc cgaagccggg ttctccttga
    15961 aatgaccgac ggtgagatag tcgaagcgct ccttgtgaaa ggcatgcagg atgagcgccc
    16021 gcgcggcttc cgtggcgtag cccatccccc aatagggctt gccgatccaa tatcccatct
    16081 cggcgtgggt cttctcggtg gcgcgatagc cgacgcagcc gatcagcgcg ccgccgcggt
    16141 cgatggtaaa ggcgacacct tcttcgccgg cctgtaggct ttggatccaa tcgaccgcca
    16201 tctcctcgct ataggggtgc gggatggttc cggtcatgct ggcgacctcg tagtcgccgg
    16261 cgagcaggct gatgctcgcc gcatcagcaa gcaccggggt gcgcaacagg agtctttcgg
    16321 tgcggatggc cacgagggca ggatacgcgg gcgcggccct tcttgtcatt gcccggctta
    16381 gcgggcatga caagcgtacg acgttagttc ggcttcaccg agacgtagac gcggcccttg
    16441 gccttggtcg cgaattcgac gtcgccctcg cgaagcgcaa acagagtgtg gtccacgccc
    16501 atgccgacgc cgtcgtcggg atgccatttg gtgccgcgct ggcgcacgat gatgttgccg
    16561 ggaatgacgc gctggccgcc cgacttcttg accccgagcc gccttccggc tgaatcgcgc
    16621 ccgttacggg atgagccacc ggctttctta tgtgccatgg tatcggtcct tacttcttct
    16681 tcgcgggctt ggccgcaggc ttcttggctg cagccttcgc cgctggcttc ttggccgctg
    16741 ccgctttggc ggctggcttc ttggctggag cggccttctt ctcaggcgcg gccttcttcg
    16801 ccgctggctt cttcgcaggc ttcgcctcgg ccttcgcttc atcggcctct ggcttcttgg
    16861 cctcgacctt ctttggctca ggacgcgcca ccttggtctg cgacggcttc ttgccgtcgg
    16921 tgaggatctc ggtgatgcgg agcagcgtca gatcctggcg atgccccttc ttgcggcggt
    16981 agttcttgcg ccgcttcttc ttgaacacga tgatcttgtc gccgcgcgac tgctccatca
    17041 cctcggcggc aaccagcgcg ccggcaatgg tcgggctgcc cgtcttcggg ctatcgccgc
    17101 caagcagcag cacctcgccg agcgtaatga tgtcgccggg ctcgcccgcg acgcgctcga
    17161 tctcgataat gtcgttcggc gccacgcgat attgcttgcc gcccgtgcgg atgaccgcga
    17221 acatgtctcg ctctcgtctc taactcttgg cttggaaagc cggccttgcc taggccgcct
    17281 tggcaggggg ccagagaaaa tggcgggttc agaggccgaa ggggccgatc ccgccaagcg
    17341 gcgcactata taaatcaaag accagaagtc aatcgaagcg ttgggcggcg aagatacgag
    17401 ccgccagttg ccgccaacgg actggcacta taaaggtgcc gctcccgact accatttgcg
    17461 catggtcgag ccttccggag aggtgccaga gtggtcgaat gggacggtct cgaaaaccgt
    17521 tgtacgggta accgtaccgt gggttcgaat cccaccctct ccgccagcta gtcctctgtt
    17581 ttccaaaaaa attccactct ccgaaattgt ttagacagtt ccgtgggtta gccccatcat
    17641 gtccgcttat tgtcccagac cggacatagc tcaggga
//
LOCUS       DATNAE010000072         3061 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2788855, whole genome shotgun sequence.
ACCESSION   DATNAE010000072 DATNAE010000000
VERSION     DATNAE010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2788855"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /gene="fixJ"
                     /locus_tag="VN518_01485"
     CDS             complement(<1..563)
                     /gene="fixJ"
                     /locus_tag="VN518_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010967648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator FixJ"
                     /protein_id="HXE03275.1"
                     /translation="MPDRIVHIVDDDEAVRQSLAFLLGSAGLTVRLYDSASAFLAGLS
                     AVKSGCLVTDMRMPDMTGIELLQQLRAKACRLPAIVITGHGDVPLAVEAMKAGAVDFI
                     EKPFDQETILHAVQAALERSFEGEGREDPAVAARLATLSERERQVLEGLVAGHPNKTI
                     AYDLGISPRTVEVYRANVMSKMQAKSLP"
     gene            complement(556..2079)
                     /locus_tag="VN518_01490"
     CDS             complement(556..2079)
                     /locus_tag="VN518_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXE03276.1"
                     /translation="MHGPIASRSESALERLFGALLDPATSRTHYWAAAAVVVIWLMLA
                     WGLHPWLGDRGSFLIFIPAVLFAAGFGGLGPGLLATALSVTLGVLLVGRGHLTQPAVL
                     ETVIFAIVGAGISWFGEQWRLTRIRDLSHTQDLRAREAHLRSILDAVPDATVVIDEKG
                     TIQSFSAAAERLFGYKESGVVGKNVSMLMPQPYRGEHDRYMQRYLATGEKRIIGIDRV
                     VTGQRKDGSTFPMKLEVGEMRPGEGRFFTGFIRDLTERQQTEDQLHDLQTELARLSRL
                     TAMGEMASTLAHEINQPLSAISNYLQGCNRLLEPVEHPNVPKIRDALAETTKQTLRAG
                     HIIRQLREFVARGETEKRPENISKLVEEASALALVGAKEEGVKTLFRFASHTDTVLVE
                     KVQIQQVLLNLMRNAIEAMQGCEPKELLVTTSAPNDDKITVSVADTGSGLSEEIAARL
                     FQPFVTTKPAGMGVGLSISKRIIEAHGGEMWAEPNPGGGAIFRFTVQSATETGGVDA"
     gene            2226..2495
                     /locus_tag="VN518_01495"
     CDS             2226..2495
                     /locus_tag="VN518_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03277.1"
                     /translation="MANKDAEMPTHHALREEVDKIAKHLHALRKDIDGLTGAVAAAGG
                     HQAERARDAAGEALAAIEDAVKRKPTQTLGIALGIGFLVGIILRR"
     gene            complement(2479..>3061)
                     /locus_tag="VN518_01500"
     CDS             complement(2479..>3061)
                     /locus_tag="VN518_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation transporting ATPase C-terminal
                     domain-containing protein"
                     /protein_id="HXE03278.1"
                     /translation="FGNMFSMAGAALFLPYLPMLPTQVLLNNLLYDVSEVGVPFDHVD
                     AEALRKPVRWNIKFIERVMLVLGPVSSLFDFLTFFALLWLFGASEPMFQTGWFIESLA
                     TQSLVIFVIRTRKAPWRSFPHPLLTALTIGVVLVGLLIPLTPLGPVFGFVVPPLGYYL
                     FLAVAVTGYLLLVEVVKRLLYRPSVRTAASAAR"
BASE COUNT          611 a          949 c          995 g          506 t
ORIGIN      
        1 ggaagcgatt tagcttgcat cttgctcatg acattggcgc gatagacctc gaccgtgcgc
       61 gggctgatgc cgagatcata ggcgatggtc ttgttgggat ggcccgcgac caagccttcg
      121 agcacctgcc gctcgcgctc cgacaaagtg gcgagcctgg cggcgaccgc tgggtcttcc
      181 ctgccctctc cctcgaagct gcgctcaagc gcggcctgca cggcatgcag aatcgtctct
      241 tgatcgaacg gtttctcgat gaaatcgacg gcgccggcct tcatcgcctc gaccgcgagc
      301 ggcacgtcgc cgtggccggt gatgacgatg gcgggaaggc ggcaggcttt ggcgcgcaac
      361 tgctgcaaca gctcgatgcc ggtcatgtcg ggcatgcgca tatcggtgac gaggcagccg
      421 ctcttgacgg ctgagaggcc ggcaagaaag gcgctcgccg agtcgtaaag ccgcacggtc
      481 aagccagccg aacccagcag gaaggcgagc gactggcgca ctgcctcatc gtcatcgacg
      541 atatgcacga tccggtcagg catccacgcc acccgtttcg gtcgccgatt gcacggtgaa
      601 gcggaagatg gcgccgccgc cgggattcgg ctcggcccac atttccccgc catgcgcctc
      661 gatgatgcgc ttggagatgg atagtccgac gcccatgccg gccggcttgg tcgtcacgaa
      721 aggctgaaac agccgcgcgg cgatctcctc ggagaggccg ctgccggtat ccgccacgct
      781 cacggtgatc ttgtcgtcgt tcggcgccga ggtcgtcacc agcaattcct tgggctcgca
      841 gccctgcatg gcctcgatgg cgttgcgcat gagattgagc agcacctgct ggatctgcac
      901 cttctccacc agcaccgtat cggtgtggct ggcgaagcgg aacagcgtct tcaccccctc
      961 ctccttggcg ccgaccaggg cgagcgcgct cgcttcctcg accagcttgc tgatattctc
     1021 gggccgcttc tcggtttcgc cgcgcgccac gaattcgcgc agctggcgga tgatgtgacc
     1081 ggcgcgcagc gtctgcttgg tggtctcggc gagagcatcg cggatcttcg gcacgttggg
     1141 atgctcgacc ggctcgagca gccggttgca gccctgcaaa tagttggaga tggccgagag
     1201 cggctggttg atctcgtgcg cgagcgtcga ggccatctca cccatggcgg tcagccgcga
     1261 caggcgagcc agctcggttt gcaggtcgtg cagttgatcc tcggtttgct gccgctcggt
     1321 gaggtcgcgg atgaagccgg tgaagaagcg gccttcgccc ggccgcatct cgccgacttc
     1381 gagcttcatg ggaaaggtcg atccgtcctt gcgctggccg gtgaccacgc ggtcgatgcc
     1441 gatgatgcgc ttctcgccgg tagcgagata gcgctgcatg tagcggtcat gctcgccgcg
     1501 ataaggttgc ggcatcaaca tcgagacgtt cttgccgacg acgccggact ccttatagcc
     1561 gaacaatcgt tctgcggccg cgctgaaaga ctggatggtc cccttctcgt cgatgacaac
     1621 cgtagcgtcg ggtaccgcgt cgaggatcga gcggagatgc gcttcgcgcg cccgcagatc
     1681 ttgcgtgtgg ctcaggtcgc gaatgcgcgt gaggcgccac tgctcgccga accaggagat
     1741 accggcgccg acaatggcga agatgacggt ctcgagcacc gcgggctgcg taaggtggcc
     1801 gcgccctaca agcaagacgc cgagcgtgac gctcagcgcc gtggcgagca gccccggacc
     1861 caggccgcca aatccggcgg caaacaacac cgccgggatg aagatgagaa aggagccgcg
     1921 atcccccagc caagggtgca atccccaggc cagcatgagc caaatcacga caacggccgc
     1981 ggccgcccaa tagtgcgtgc gcgaggtcgc gggatcgaga agcgcgccaa aaaggcgctc
     2041 gagagcgctt tcggagcgcg aggcaatcgg gccatgcatg acgaattcta tccgttcctg
     2101 acgcggcgcg gtacataagc atccgcccag gacgtttgat ttccgtcaac gcccgagacg
     2161 gcttgaccgg gaaagcttaa catccaccaa tcccggagac tgaagctggc ttaggagtga
     2221 gcgagatggc caacaaagac gccgaaatgc cgacccatca tgcgctgcgc gaagaggtcg
     2281 acaagatcgc caagcatctg cacgccttgc gcaaggatat cgacggcttg accggcgcgg
     2341 tcgccgcggc gggcgggcat caggcggaac gcgccaggga tgcggcgggc gaagcgttgg
     2401 cggcgatcga ggacgcggtg aagcgcaaac cgacccaaac gctcggcatc gcgctgggga
     2461 tcggctttct cgtcggcatt atcttgcggc gctagcggct gttcgcacgc tcgggcggta
     2521 aaggagccgc ttgaccactt caacgagcag cagatagccg gtcacggcca ccgccagaaa
     2581 cagataatag ccgagcggcg gaacgacaaa gccgaagacg ggacctagcg gcgtcagcgg
     2641 aataagcagc cctaccaaca cgaccccgat ggtcaacgcg gtcagcaatg ggtggggaaa
     2701 gctccgccac ggcgccttgc gggttcttat gacaaatatg acaagggact gggtcgccag
     2761 ggactcgata aaccagccgg tctggaacat cggctcgctc gcgccaaaca gccaaagcaa
     2821 tgcgaaaaag gtgagaaaat cgaagagcga gctgaccggt ccgagtacga gcatgacgcg
     2881 ctcaataaac ttgatgttcc aacgcaccgg cttgcgcagc gcttcggcgt ccacgtgatc
     2941 gaacggcacg cccacttccg agacatcgta gaggaggttg ttgaggagga cctgagtcgg
     3001 caacatgggc agatacggca agaaaagagc cgcgcccgcc atgctgaaca tattgccgaa
     3061 g
//
LOCUS       DATNAE010000073         3010 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_1586793, whole genome shotgun sequence.
ACCESSION   DATNAE010000073 DATNAE010000000
VERSION     DATNAE010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1586793"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="VN518_01505"
     CDS             complement(<1..762)
                     /locus_tag="VN518_01505"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017964507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HXE03279.1"
                     /translation="MSKIKVTNPVVDLDGDEMTRVIWQLIKDKLIKPYLDIELRTFDL
                     SIENRDLTDDQVTVDAAYAIKDYGVGIKCATITPDEARVKEFGLKKMWKSPNGTIRNI
                     LGGVIFREPIICRNVPRLVPGWTKPIVIGRHAYGDVYRATDFRVPSAGKLTISFVGED
                     GARIEHEVHEFKGPGVALAMYNVDDSVRDFARASLNYGLIRNYPVYLSTKNTILKAYD
                     GRFKDIFAEVYQAEFREEFERRGLTYEHRLIDDMVA"
     gene            913..1731
                     /locus_tag="VN518_01510"
     CDS             913..1731
                     /locus_tag="VN518_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HXE03280.1"
                     /translation="MPKSRAKSHPVGLAPAVILVEPQLGENIGFAARAMANFGLTELR
                     LIAPRDGWPSDRARAAAAGADAIIDAATVYPSLEAAIGDLTFLLATTARPRGMVKPVL
                     TPAGAARDLAARGAKGERTGVMFGPERSGLDNDAISLADAIVTAPVNPSFASLSLPQT
                     VLLVGYEWLQARGAQPSLGRATKCDGPAAEGLDLSGTRQATRAELLALFEHLESELDR
                     CGFLRPPEKRPTMVRAIRNMFHRMGATEQDVRTWRGIVASLSGSNRRKGKGEGS"
     gene            1808..2572
                     /locus_tag="VN518_01515"
     CDS             1808..2572
                     /locus_tag="VN518_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03281.1"
                     /translation="MRVAWIEWSGAALTAVVALLAGGPLNAADQSGVHYVAEELGNRT
                     KPAGAAQGAPKGLSDSAVRVMSTFALSIVPDQVPAGPGGQMVKLDKSDPNKYLIPLDD
                     ARQIIRVATRSAYAEVCQLPEFEKANFDALIRGEKLRGWTAEQMMFIHALHTFATSYF
                     AGNVKITESDAGKSGDLAKEGAAAANAAKGDATTITTKKLECAPGQKEKVMAAINTYV
                     QSVPAAQAVPAAPEPPAAAAAPQAIPAPAPVAGGAN"
     gene            2745..>3010
                     /locus_tag="VN518_01520"
     CDS             2745..>3010
                     /locus_tag="VN518_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HXE03282.1"
                     /translation="MTKRVNAKHKIDRRLGENIWGRPKSPYNKREYGPGEHGQRRRGK
                     LSDYGQQLRAKQKLKGYYSNIGERQFSRVYKEAARLKGSTSEHL"
BASE COUNT          556 a          980 c          964 g          510 t
ORIGIN      
        1 cgccaccatg tcgtcgatca gccggtgctc gtaggtcagc ccgcgccgct cgaactcctc
       61 gcggaactcc gcctgataca cctcggcgaa gatatccttg aagcgcccgt cataggcctt
      121 gaggatggtg ttcttggtgg agaggtagac cggatagttg cggatcagcc cgtaattcag
      181 gctggcccgc gcgaagtccc tgaccgagtc gtcgacgttg tacatggcga gcgccacgcc
      241 ggggcctttg aactcatgca cctcatgctc gatgcgggcg ccgtcctcgc cgacgaaact
      301 gatggtgagc ttgccggcgc tgggcacccg gaaatcggtg gcgcggtaga catcgccgta
      361 agcatggcgg ccgatgacga tgggcttggt ccagcccggc acgagacgcg gaacgttgcg
      421 gcagatgatc ggctcgcgga agataacccc gccaaggatg ttgcggatgg tgccgttcgg
      481 cgacttccac atcttcttga gaccgaactc cttcacccgc gcttcgtcgg gggtgatggt
      541 cgcgcatttg atgccgacgc cgtagtcctt gatggcgtag gccgcgtcga cggtgacctg
      601 atcgtcggtc aagtcgcggt tctcaatgga aagatcgaag gtgcgaagct cgatatcgag
      661 atagggcttg atcagcttgt ccttgatcag ctgccagatg acccgagtca tctcgtcccc
      721 gtcgagatcg acgacggggt tcgtcacctt gatcttgctc actggaagtc tccggcggga
      781 tagttgaagg cgtatcgctc tcgctttcgg ctcaccattg cggcctgctc gcccctatag
      841 catcgtgttc cgatgatgaa aacaagagcc gaggccgaac cgagaaggcg gccaaaaccg
      901 aagcgacggg cggtgcccaa atcgcgggca aaatcccacc ccgttggcct cgcgcccgcg
      961 gtgattctgg tcgagccgca gcttggcgag aatatcggct tcgccgcgag ggccatggcc
     1021 aatttcggtc tgaccgagct tcgcctcatc gcccctcgcg atggctggcc gagcgacaga
     1081 gcgcgcgccg ccgctgctgg cgccgatgcc atcatcgacg ctgcgacggt ctatccgagc
     1141 cttgaggctg caatcggcga tctcaccttc ctgctcgcca ccacggcgcg gccgcgcggc
     1201 atggtgaagc ctgtactgac gcctgccggc gctgcccgcg acctcgccgc gcgcggggcg
     1261 aagggcgagc gcaccggcgt gatgttcggg cctgagcgaa gcggcctcga caacgatgcg
     1321 atttctctcg ccgacgccat cgtgaccgca ccggtcaatc cgtctttcgc ctccttgagc
     1381 ctgccccaga ccgtgctgct cgtcggctat gaatggcttc aggcgcgagg cgcgcagccg
     1441 tcgctcggcc gcgccaccaa atgcgatggg ccggctgcgg aagggctcga tctttccggc
     1501 acgcggcagg cgacccgcgc cgagctgctc gcgcttttcg agcacctgga aagcgagctc
     1561 gaccggtgcg gtttcctgcg tccgccggag aagcggccca ccatggtgcg cgccatccgc
     1621 aatatgtttc atcgcatggg cgcgaccgag caggacgtcc gcacctggcg cggaatcgtg
     1681 gcctcgctca gcggtagcaa ccggcgcaag ggcaaggggg aggggtcata gttctgcccc
     1741 gtttctgcta ttgcgatgac ccttgaggat gattagcttc cgggcaccgc agggagaggg
     1801 gcaatcgatg cgagtcgcgt ggattgaatg gagcggggcc gcgctcacgg cagtagtcgc
     1861 gctcttggcc ggaggaccgc ttaacgccgc ggaccagtcc ggcgtccact acgttgccga
     1921 ggagctgggc aaccgcacca agccggcggg tgcagcgcaa ggcgctccca aagggctgag
     1981 cgacagcgcc gtgcgcgtga tgtcgacctt cgcgctctcc attgttcccg atcaggtgcc
     2041 ggcagggcct ggcgggcaga tggtcaagct cgataaatcc gaccccaaca aatatctcat
     2101 ccccctcgac gatgcgcgcc aaatcatccg cgtcgctacc cgctcggcct atgccgaggt
     2161 ctgccagctt ccggagttcg aaaaggccaa tttcgacgcg ttgattcggg gcgagaagct
     2221 gcgcggctgg accgccgagc agatgatgtt catccacgcc ctacacacct tcgcgacctc
     2281 ctacttcgcc ggcaacgtca agatcaccga gtccgacgcc ggcaaatcgg gcgacctggc
     2341 caaagagggt gccgcggcgg ccaatgccgc caagggcgac gccacgacca tcaccacgaa
     2401 gaaactcgaa tgcgcgcccg ggcagaagga aaaggtaatg gcggcgatca acacctacgt
     2461 gcagtcggta cccgccgcgc aggcggtacc agcagcgccc gagcccccgg cagcggcggc
     2521 ggcgcctcag gccatcccgg cgccggctcc ggtcgccggc ggcgccaatt gacactttcc
     2581 gcacattccg tgtaacagag cgcgggcggc gcttcctccc tcaaggtttg gagcgctcga
     2641 ggccttgaac gcaggcttcc gcccttcatc gcaaaatggt ttaaggagct gtcgcggccg
     2701 acaagcccgc gagcaagccc aattcggctt tggagctttt cagaatgacg aaacgcgtga
     2761 acgcgaagca taagatcgac cgcaggctcg gcgagaatat ctggggccgg ccgaagagcc
     2821 cttacaacaa gcgtgaatac gggccaggcg agcacggcca gcgccgccgc ggcaagcttt
     2881 ccgactacgg ccagcagctg cgcgccaagc agaagctgaa gggctactac agcaacatcg
     2941 gcgagcgcca gttcagccgg gtctacaagg aggcggcgcg cctcaaaggt tcgacctccg
     3001 agcacctgat
//
LOCUS       DATNAE010000074         3011 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2184278, whole genome shotgun sequence.
ACCESSION   DATNAE010000074 DATNAE010000000
VERSION     DATNAE010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2184278"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="VN518_01525"
     CDS             complement(<1..792)
                     /locus_tag="VN518_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HXE03283.1"
                     /translation="MSKRSPGQKQNTRLAHAGREPERFHGFVNPPIYRGSTVLFPTAA
                     ALEANEQEYTYGRLGTPTVRALEEAIAELEGGHATRLTPSGLSAIVATFLAFLSAGDH
                     VLVTDAVYRPTRRFCDQVLARLGVEITYYDPLIGAGIKDLIKPNTKVVFAESPGSQTF
                     EVQDIPAIAKAVHEAGAVVVLDNTWATPLFFKSFAHCADVSIQAATKYIVGHADAMLG
                     AITASKAIWPAIARTHEELGLCVGPEDAYLGLRGLRSLGVRLARHQ"
     gene            complement(828..1682)
                     /gene="sseA"
                     /locus_tag="VN518_01530"
     CDS             complement(828..1682)
                     /gene="sseA"
                     /locus_tag="VN518_01530"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HXE03284.1"
                     /translation="MTERSKSWLTETDELAAELDAPDIVIIDASWHMPGDGKNARAEY
                     LEEHIPGAIFFDIDEIADTKSSLPHMLPPPEKFSSRMRSMGIGDGSRIVVYDAAGIYS
                     AARVWWTFRVMGVEDVTVVNGGLPKWKREGLPLESGEPRLRSARHFTSRRNADLVRDV
                     FDMKALIKDHSAEIVDARSVERFSGRAQEPRAGLRSGHIPGAHNLPYARLLRQDGTLK
                     SPAQIEALFSEAGVDLSKPVVTSCGSGITASVLALALAQIGHRRTAVYDGSWSEWGAD
                     QSLPIETG"
     gene            complement(1696..2730)
                     /locus_tag="VN518_01535"
     CDS             complement(1696..2730)
                     /locus_tag="VN518_01535"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015929385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HXE03285.1"
                     /translation="MHYRDDIIEAIGNTPLIKLKRASEETGCLILGKAEFMNPGQSVK
                     DRAALFIIRDALASGRLQPGGTVVEGTAGNTGIGLALVGNALGLKTVIVIPDTQSDEK
                     KDMLRLAGAELIEVPAVPYKDPNNYVKYSGRLAEQLAATAPHGAIWANQFDNVANRQG
                     HIETTAPEIWNDMQGKVDGFICAVGTGGTLAGVSMGLKAKNKDITIGLADPMGASLYN
                     YYEHGELKSEGSSITEGIGQGRITANLEGAVIDEAYQIPDAEALPVLFNLLSEEGLCL
                     GGSSGINVAGAIRLARDMGPGHTIVTVLADYGTRYQSRLFNPTFLREKGLPVPAWLDA
                     REPATPAVCV"
BASE COUNT          499 a          945 c          997 g          570 t
ORIGIN      
        1 ttgatgccgg gcaagccgca cgccgaggct gcgcagcccg cgcaagccga gataggcatc
       61 ctcggggccg acgcagaggc caagctcctc gtgggtgcgg gcgatcgcgg gccagatggc
      121 cttgcttgcg gtgatggcgc cgagcatggc gtcggcatga ccgacgatat atttggtcgc
      181 cgcttggatc gacacgtcag cacagtgcgc gaaggatttg aagaagagcg gcgtcgccca
      241 ggtattgtcc aacacgacca cggcgccagc ttcgtgcacg gctttggcga ttgccggaat
      301 gtcctgcacc tcgaaggtct gtgagccagg cgactcggcg aacacgacct tggtgttcgg
      361 cttgatgagg tccttgatgc ccgcgccgat gagcggatcg taataggtga tctccacgcc
      421 aaggcgcgcg agcacctggt cgcagaagcg gcgcgtcggc cgatagacgg cgtcggtcac
      481 gagcacatgg tctcccgccg agaggaacgc gaggaaggtg gcgacgatgg ccgagaggcc
      541 cgacggggtc agccgcgtgg cgtgaccgcc ctcgagctcg gcgatcgctt cctcgagcgc
      601 ccgcaccgtg ggcgtgccaa gccgcccata ggtgtattcc tgctcgttgg cttcgagcgc
      661 cgcggcggtc ggaaacagaa cggtggagcc gcgatagatc ggcggattga cgaagccgtg
      721 gaagcgttcc ggctcgcgtc cggcgtgtgc cagccgcgtg ttttgctttt gacctggtga
      781 ccgtttgctc atggccgtca ccgcaagatg cgcgcgtgca tcggcgtcta gcccgtctcg
      841 atgggcaggg attgatcggc gccccactct gaccaggacc cgtcatagac cgccgtgcgg
      901 cgatggccga tctgggccag cgccagagcg agcacgcttg ccgtgatgcc cgagccgcag
      961 cttgtcacca cgggcttgga gagatcgacg ccggcctcgc tgaacagagc ctcgatctgg
     1021 gcggggctct tgagcgtgcc gtcctgcctg agcagccgtg cgtaaggaag gttgtgggcg
     1081 ccgggaatgt ggcccgagcg aagcccggca cgcggctctt gcgcgcgccc ggaaaatctc
     1141 tcgaccgatc gggcgtcgac gatctctgcc gaatggtcct tgatcagtgc tttcatatcg
     1201 aacacgtcgc gcacgagatc ggcgttacgc cgcgaggtga agtgccgcgc gctgcgaagc
     1261 cttggctcgc cgctctccag cggcaggccc tcgcgcttcc atttcggcag accgccgttc
     1321 accacggtca catcctcgac acccatcacg cggaacgtcc accacacccg cgccgcgctg
     1381 taaattcccg ccgcgtcgta gaccacgatc ctcgacccgt cgccgatccc catgctgcgc
     1441 atgcgcgagg agaacttctc cggcggcggc agcatgtgcg gcagactgct cttggtgtcg
     1501 gcgatctcgt cgatgtcgaa gaagatggca cctgggatgt gctcctcgag atattcggcc
     1561 cgcgcgttct tgccgtcgcc gggcatgtgc caggaggcat caatgatgac gatgtcgggc
     1621 gcatcgagct cggccgcgag ctcgtcggtc tcggtcaacc atgacttgga gcgttcggtc
     1681 atttccaatc ccaatctaga cgcacactgc cggtgtcgct ggttcgcggg cgtcgagcca
     1741 ggcgggaacc ggcagaccct tctcacgtaa gaatgttggg ttgaatagtc tgctttggta
     1801 gcgggtgccg taatcggcca gcaccgtgac gatcgtatga ccggggccca tgtcgcgggc
     1861 aaggcggatg gcgccggcaa cattgatgcc gctcgacccg ccgaggcaca gcccttcctc
     1921 tgagagcagg ttgaacaaga ccggcagcgc ctcggcgtcg gggatctgat aggcctcgtc
     1981 gatcaccgca ccttcgagat tggcggtgat acggccctgg ccaattccct cggtgatcga
     2041 gcttccttcc gatttgagct cgccgtgctc gtagtaattg tagagcgaag cgcccatcgg
     2101 gtcggcgagg ccgatggtga tgtccttgtt cttcgctttc agccccatcg agacgccggc
     2161 gagcgtgccg ccggtgccga cagcgcagat gaaaccgtcg accttgcctt gcatgtcgtt
     2221 ccagatctcg ggcgccgtcg tctcgatatg gccctggcgg ttggcgacat tgtcgaactg
     2281 attggcccag atggcgccgt gcggcgcagt cgcggcaagc tgctcggcga ggcgcccgga
     2341 atatttcacg tagttgttcg ggtccttgta ggggaccgct gggacctcga tcagctcggc
     2401 gccggcaagc ctcagcatgt ccttcttctc atccgactgc gtgtcgggaa tgacgatgac
     2461 cgtcttcagc ccaagcgcgt tgccgacgag cgctaagccg atgccggtat tgcctgccgt
     2521 tccttccacc accgtgccgc ccggctgaag gcgccccgag gcgagcgcgt cgcggatgat
     2581 gaaaagggcg gcgcggtcct tcaccgactg gccagggttc atgaactcgg ccttgccgag
     2641 gatcaagcaa ccggtctctt ccgaggcgcg cttgagcttg atcagcgggg tgttgccgat
     2701 cgcctcgatg atgtcgtcac ggtaatgcac gggaaggtcc aagttttttc tggccgaggc
     2761 ggcaacctaa agagaccggc ccgcccctgc aaggtaccgg attttctagg ttcggcgctt
     2821 tgtcgcaagg cttttgccaa gatcgccgtc aaatcgctaa tgcaacgcga cagtggcgac
     2881 ggcgcgggca atcttctcgg ggctccaggg cttgacctcg cctggcgggt cgagtggcga
     2941 ggggatgtcg gtgccttgcc ccgcccccga aagcgtcacg ttgcccgctt gattgaacac
     3001 ggtgatctcg c
//
LOCUS       DATNAE010000075         3814 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_980471, whole genome shotgun sequence.
ACCESSION   DATNAE010000075 DATNAE010000000
VERSION     DATNAE010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980471"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="VN518_01540"
     CDS             <1..794
                     /locus_tag="VN518_01540"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HXE03286.1"
                     /translation="FGAMTDIDAPMRYAEPVADRRGTEGSFALVVLLALGLAIAAVAL
                     AVLSREMAEPFVLAILAVLAVVGVFCLFAGAVGILHFGGRHARNDVTKAFVDNLPQGA
                     LIADSGGRVLYANESYRDLLGLETDAIVPAPDRAFAGNPHLAECIFRLSRAAQQGRSL
                     KEEFRLPPPGEAEDETGIAPRWFRISVQPMPPDPRSGRKGALTVWQVDEITMDRAREE
                     ASFAKVQSAIAYLDSAPAGFFTADAEGTIEYLNATLAQWLGLDLT"
     gene            828..2561
                     /locus_tag="VN518_01545"
     CDS             828..2561
                     /locus_tag="VN518_01545"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXE03287.1"
                     /translation="MSADSAALIARSGRGQSQGATRRFDIDLVKADGTSLPVRVLHRL
                     PRPGSSGALAHALVLSRGPGEAEEAGAAELRFARLFHSAPIAIATVDGEGRISATNAA
                     FMRLFGAADEGRKVLLESLVEPESHPALRRALDAALARQSQIGPVDIAFAGDRDRSGR
                     IYLSPIEQTAGEGEAAIAYAIDITEQRALEMQIAQGQKMQAVGQLAGGIAHDFNNMLT
                     AIIGFSDFLLMNHRPTDPAFQDIMNIKQNANRAAGLVRQLLAFSRQQTLRPQKLALGD
                     VLSELSILLSRLLGENVELKLEQASDLWPVKADLHQFEQVIINLAVNARDAMPDGGKL
                     VIRTANVGESQVRELGNKHLPASDYVLIEVSDTGSGMTPEVMLKIFEPFFSTKEIGRG
                     TGLGLSTVYGIVKQTGGYVFPESEPGRGTTMRVYLPRYIEAIAEAEAEPEKIERRPEK
                     PKDLTGQGTVLLVEDEDAVRSFAARALGQRGYNVLQATTGVEALEVFASHQGEVDLVV
                     SDVVMPEMDGPTLFEKLRRQRPDLKVIFISGYAEDAFRQHLAENEDFMFLQKPFDLKE
                     LAAAVKAALQS"
     gene            2657..>3814
                     /locus_tag="VN518_01550"
     CDS             2657..>3814
                     /locus_tag="VN518_01550"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HXE03288.1"
                     /translation="MPDYEVVPEEEAGQVSRRRRAKPRMPAFTIERYGGRLIYFWFGM
                     RFGALLKLFKRGNYAFTLNTIPDTLLLFLWVPWNSVLYLLSEAKYKKAAEAIPLEPPP
                     IFVIGHWRTGTTLLHDLFSVDPNLAYPTTFECFFPHHFLLTEKVLSKVMRGLLPKKRP
                     QDDVPVGFDRPQEEEFGMMMLGEGTPYVTHAWPRFGPADTDYLDFKGVSEAEKKKWAD
                     AYMWFYRRLLLKHGGKRLVMKTPANAARLKLLTELFPDARYVYLARNPLHVFPSTVKL
                     WRALYSVQGLHNPPNLDPWLDDYVLDMFARLTEDYEEDRHLIPKDRLVELRYEDFAKD
                     PVASMRDIYSRLDIPGFDQAEAPMREFLAGRSEHKVSRYDLPLALKRKVVER"
BASE COUNT          638 a         1326 c         1202 g          648 t
ORIGIN      
        1 aatttggcgc catgaccgac atcgatgcgc ccatgcgtta tgccgagcct gtcgccgacc
       61 gcagagggac ggagggcagc ttcgctctcg tggtgctgct cgcgctcggg ctcgcgattg
      121 ccgccgtcgc tcttgccgtc ctgtcgcgcg aaatggcgga gccgttcgtg cttgccatcc
      181 tcgcagtcct cgccgtggtt ggcgtgttct gcctcttcgc cggcgccgtc ggcattctgc
      241 atttcggcgg ccggcacgcg cgcaacgacg tcaccaaggc ctttgtcgac aacctgccgc
      301 agggcgcgct gatcgccgac agcgggggcc gcgtgctcta cgccaacgag tcctatcgcg
      361 atctgctcgg cctcgagacg gacgcgatcg tgccggcgcc cgaccgcgcc ttcgccggca
      421 acccgcatct tgccgagtgc atcttccgcc tttcccgcgc ggcgcagcag gggaggagcc
      481 tcaaggagga attccgcttg ccgccgcccg gcgaagccga ggacgagaca ggcattgcgc
      541 cgcgctggtt ccgcatctcc gtgcagccga tgccgcccga cccgcgctct ggccgcaaag
      601 gcgcgctcac cgtgtggcag gtcgatgaga tcaccatgga ccgcgcgcgc gaggaggcga
      661 gcttcgccaa ggtgcagtcg gccatcgcct atctcgacag cgcgccggcc ggcttcttca
      721 ccgccgacgc cgagggcacc atcgaatatc tcaacgcgac gctcgcgcag tggcttggcc
      781 tcgacctcac ctaggtcgcc ggccgcgcgc tcaagctcga agcgatcatg tccgccgaca
      841 gcgcggcgct gatcgcccgc agcggccgcg ggcagagcca gggcgccacg cgccgcttcg
      901 atatcgatct ggtcaaggcc gacggcacca gtctgccggt gcgcgtcctg caccgcctcc
      961 cgcgccccgg atcgagcggg gccttggcgc atgcgctggt tttgagccgc ggtcccggcg
     1021 aggccgagga ggccggcgcc gccgagctca gatttgcccg cctgttccat tcggcgccga
     1081 tcgccatcgc cacggtcgac ggcgaaggcc gcatcagcgc gaccaatgcc gccttcatgc
     1141 gcttgttcgg cgccgccgac gagggcagga aagtgctgct ggaatcgctg gtcgagccgg
     1201 agagccatcc ggcgctccgc cgcgcgctcg acgccgcgct tgcccgccag agccagatcg
     1261 gtcccgtcga catcgccttt gccggcgacc gcgaccgcag cggcaggatc tatttgagcc
     1321 ccatcgagca gaccgccggc gaaggcgagg cggccatcgc ctacgccatc gacatcaccg
     1381 agcagcgcgc gctcgagatg cagatcgcgc aagggcagaa gatgcaggcg gtcggccagc
     1441 tcgccggcgg catcgcgcac gacttcaaca acatgctgac ggcgatcatc ggcttctccg
     1501 acttcctgtt gatgaaccat cggccgaccg accccgcctt ccaggacatc atgaacatca
     1561 agcagaacgc gaaccgggcc gcgggcctgg tgcgccaact gcttgccttc tcgcgccagc
     1621 agaccctgcg cccgcagaag ctcgcgctcg gcgacgtgct gtcggagctc tccattttgc
     1681 tcagccgcct gctcggcgag aatgtcgagc tcaagctcga gcaggcgtcc gacctctggc
     1741 cggtgaaggc ggatctgcat caattcgagc aggtgatcat caaccttgcc gtcaatgcgc
     1801 gcgacgccat gcccgacggc ggcaagctcg tgatccgcac cgccaatgtc ggcgagagcc
     1861 aagtccgcga gctcggcaac aagcacctgc cggccagcga ctatgtgctg atcgaggtga
     1921 gcgacaccgg cagcggcatg acgcccgagg tgatgctgaa gatcttcgag ccgttcttct
     1981 ccactaagga gattgggcgc ggcaccgggc tcggcctctc caccgtctac ggcatcgtca
     2041 agcagaccgg cggctacgtc ttccccgaga gcgagccggg tcgcggcacc accatgcgcg
     2101 tctacctgcc gcgctatatc gaggcgatag ccgaagccga ggccgagccg gagaagatcg
     2161 agcggcgtcc cgagaagccg aaagacctga ccgggcaggg gaccgtgctc ctggtcgagg
     2221 acgaggatgc cgtgcggagc ttcgccgccc gcgcgctcgg ccagcgcggc tataacgtgc
     2281 tgcaggcgac caccggcgtc gaggcgctcg aggtgttcgc cagccaccag ggcgaggtcg
     2341 atctcgtggt gagcgacgtg gtgatgcccg agatggacgg acccactctg ttcgagaagc
     2401 tccgccgcca gcgccccgac ctcaaggtga ttttcatttc cggctatgcc gaggatgcct
     2461 tccgccagca tctcgccgag aacgaggact ttatgttcct gcagaagccc ttcgatctca
     2521 aggaactcgc cgccgccgtg aaggccgcgc tgcagagcta gcagaaagcg ccgaaaggcc
     2581 ttcccttcgc cattgatcgc ggctaccacg cgggagacgg accgatccca atccatttgc
     2641 cctttggagc gtttgcgtgc ctgattacga ggtcgtgccg gaggaagaag ccggccaagt
     2701 ttcccgccgc cgccgcgcca agccgcgcat gccggcgttc accatcgagc gctatggcgg
     2761 gcggctgatc tatttctggt tcggcatgcg cttcggcgcg ctgctgaagc tgttcaagcg
     2821 cggcaattac gccttcacgc tcaacaccat ccccgacacg ctgctgctgt ttctctgggt
     2881 gccgtggaac tcggttctct atttgctctc cgaggcgaag tacaagaagg ccgccgaggc
     2941 gatcccgctg gagccgccgc cgatcttcgt catcggccac tggcgcaccg gcaccacgct
     3001 gctgcacgat ctcttctcgg tcgatcccaa cctcgcttat ccgaccacct tcgagtgctt
     3061 cttcccgcat catttcctgc tgacggagaa ggtgctgtcc aaggtgatgc gcgggctcct
     3121 gcccaagaag cgcccgcagg acgatgtccc ggtcggcttc gaccgcccgc aggaagaaga
     3181 gttcggcatg atgatgctgg gcgagggcac gccttatgtc acccacgctt ggccccgttt
     3241 cggccctgcc gacaccgact atctcgactt caagggcgtg agcgaggccg agaagaagaa
     3301 atgggccgac gcctatatgt ggttctaccg ccggctcctg ctgaagcatg gcggcaagcg
     3361 cctggtgatg aagacgccgg ccaatgccgc gcggctcaag ctgctcaccg agctgtttcc
     3421 cgatgcgcgc tacgtctatc tggcgcggaa tccgcttcac gtgtttccct cgacggtgaa
     3481 actgtggcgc gcgctctatt cggtgcaggg cctgcacaat ccgcccaatc tcgatccctg
     3541 gctcgacgac tatgtgctcg acatgttcgc ccggctcacc gaggactacg aggaggacag
     3601 gcacctcatt cccaaggacc gcctggtcga gctccgctac gaggatttcg ccaaggaccc
     3661 ggtggcgtcg atgcgcgaca tctacagccg cctcgatatc ccgggtttcg atcaggccga
     3721 agcaccgatg cgcgaatttc tcgccgggcg ctccgagcat aaggtctcgc gctacgacct
     3781 gccgcttgcg ctgaaacgca aggtggtcga gcgg
//
LOCUS       DATNAE010000076         3272 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_380517, whole genome shotgun sequence.
ACCESSION   DATNAE010000076 DATNAE010000000
VERSION     DATNAE010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380517"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VN518_01555"
     CDS             <1..253
                     /locus_tag="VN518_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HXE03289.1"
                     /translation="RKRIVPVEGVSALPASGTSIEAGGVPIGTLGSVSGAAGLGLIRL
                     DRAEEALAQARVLTAGGVKIALRRPAFARFAVPVAEALA"
     gene            250..855
                     /locus_tag="VN518_01560"
     CDS             250..855
                     /locus_tag="VN518_01560"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HXE03290.1"
                     /translation="MSGKPAPGRCPWPGTDPLYVAYHDDEWGVVERDDRALYEKLVLD
                     GFQAGLSWITILRKREAFRRAFDGFAPEKIARYSKKKVEALVQDAGIVRNRMKIEGAV
                     ASARAWLEIMEKSAGFSPFVWKFVEGKPIINRYKTMSEIPAETEISRIISKELKAHGF
                     KFCGPTIIYAFMQGTGMVNDHLTSCPRHAACVKLARAPFNP"
     gene            complement(845..1087)
                     /locus_tag="VN518_01565"
     CDS             complement(845..1087)
                     /locus_tag="VN518_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008125023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HXE03291.1"
                     /translation="MAKLARYSLSHDEKKKRWVLKHEGTGQTVKTFATKAAGTKGGVL
                     ERAVKGTGSVRIRKRNGQIQEERTFPRNADPRKSKG"
     gene            1302..1871
                     /locus_tag="VN518_01570"
     CDS             1302..1871
                     /locus_tag="VN518_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009798968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD family hydrolase"
                     /protein_id="HXE03292.1"
                     /translation="MLSGRRLDLLDPSPLDIEIEDIAHGLARVARWNGQTDGEHAFSV
                     AEHSLLVEKIVTELEPKLDRRFRLGALIHDAPEYVIGDLISPFKAALSLDYRAFEARL
                     LAAIHLRFGLPAELPDDVTRLIKRADKIAAYYEAIRLAGFSRAEARRFFGQPRGLSPA
                     LVDRLSHLSPLPASQGEAEFLKKFNELRG"
     gene            1960..2487
                     /locus_tag="VN518_01575"
     CDS             1960..2487
                     /locus_tag="VN518_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HXE03293.1"
                     /translation="MSPETIYVAPLSLVETTVADANVSHLVTLINGETLINTPPSIVP
                     DRHLRLAMNDICEPQPGLVLPCETHVADLIGFARDWDRQAPLLIHCWAGISRSTAAAF
                     ISLCALNPEGSELELARVLRRASPTAYPNRLLVALADEILTRDGRMINAVEEIGRGKI
                     AEEAEVFALPALLAA"
     gene            2496..>3272
                     /locus_tag="VN518_01580"
     CDS             2496..>3272
                     /locus_tag="VN518_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD regulator"
                     /protein_id="HXE03294.1"
                     /translation="MAEPRRKATPAQETRNDDPQIELGLNAAIVTVREGQPYILVTRP
                     GTAGSDAWDALPFGPFAPRAHRTLEIGLREWVKRQTGLALGYVEQLYTFGDRGRHAEP
                     GGAHTLSVGYLALTRGAGDELLGSHWSPWYAYFPWEDWRKGKPPILSSQIEPKLKEWA
                     ELPPLPDEPVRPLRRSERLRTSFGIGGSWDEEKVLDRYELLYEAGLVAEAARDDRPAA
                     AQWSDLPDLGRPMAFDHRRILATALGRLRGKLKYRPVVFEL"
BASE COUNT          571 a         1068 c         1032 g          601 t
ORIGIN      
        1 gcggaagcgc atcgtgcccg tcgagggtgt gagcgccctc cccgcttccg gcacgagtat
       61 cgaggccggc ggcgtgccga tcggaacgct cggctcggtg agcggcgccg ccgggctcgg
      121 cctgatccgc ctcgaccggg ccgaggaggc tttggcgcaa gccagggtgc tcacggcggg
      181 aggggtcaaa atcgcactgc gccgtccggc cttcgcgcgc ttcgccgttc ccgttgccga
      241 ggctcttgca tgagcggcaa gccggcaccg ggtcgctgcc catggcccgg cacagacccg
      301 ctttatgtcg cttatcacga cgacgaatgg ggcgtggtcg agcgcgatga tcgcgcgctc
      361 tacgagaagc tcgtgctcga cggatttcag gcggggctgt cctggatcac gatcttacgg
      421 aagcgggagg ctttccgccg cgcgttcgac ggcttcgccc ccgagaagat cgcgcgctac
      481 tcgaagaaga aagtcgaagc cctggtgcag gacgcaggca tcgtgcgcaa tcgcatgaag
      541 atcgagggcg cggtcgcctc ggcgcgcgcc tggctcgaga tcatggagaa gagcgccggc
      601 ttcagcccgt tcgtatggaa gttcgtggag ggcaagccga tcatcaatcg ctacaagacg
      661 atgagcgaga tccccgccga gacagagatc tcgcgcatca tctcaaagga gctcaaggcg
      721 catgggttca aattctgcgg gccgacgatc atctacgcct tcatgcaggg gaccggcatg
      781 gtcaacgacc acctgacttc ctgcccccgc cacgcggctt gcgtaaaact cgcgcgagca
      841 ccatttaacc cttagatttg cgtggatccg cgttgcgcgg aaaggtgcgc tcttcttgga
      901 tttgaccgtt gcgctttcta atgcggaccg agcccgttcc cttgacggcc ctttcgagga
      961 cgccgccctt ggtccccgca gccttggtgg cgaaggtctt gacggtttgg ccggtgcctt
     1021 catgtttgag cacccagcgc ttcttcttct catcgtgcga caatgaatat cttgccagct
     1081 ttgccatatg cgtctcccag tgacagattc gattcagctg tactctgaca attacagtgc
     1141 catgcatgga attgcgagcg gcattcgtcc aattcttcgt gacttctgcc gtgatttaag
     1201 tcaatgccga tgagtcgcgc ccggactgat ccgcaaagat gaaacccgct cgcaccccgc
     1261 aaaacctgga acccaatgcg gtcccgcgcg cctggcagcg catgctttcg gggcgccggc
     1321 tcgacctgct cgacccctcg ccgctcgata tcgagatcga ggatatcgcg catggtctcg
     1381 cgcgcgttgc ccggtggaac ggccagacgg acggcgagca tgccttctcg gtggcggagc
     1441 atagccttct cgtcgagaaa attgtgaccg agctcgagcc caagctcgac cggcgcttca
     1501 ggctgggggc actgatccac gacgcgccgg aatatgtcat cggcgatctg atcagcccgt
     1561 tcaaggcggc cttgtcgctc gactatcgcg ccttcgaggc gaggctgctg gccgccattc
     1621 acctgcgctt cggactacca gcggagttgc ctgacgatgt caccagattg attaagcgcg
     1681 ccgataagat cgcggcttat tatgaggcga ttcgtcttgc cggtttttcc cgggcggagg
     1741 cgcggcgctt tttcggccag ccacggggct tgagccccgc tctggtcgac agactgtcgc
     1801 acttaagccc gcttcctgcg tcacagggag aggctgagtt cctcaagaaa ttcaacgaac
     1861 tccgaggata gtgggcgcgc aagacgcccg atgctgaagg ccctataaga aatgaccacc
     1921 ctgattttgc ccagagaaac gactagagtg ccgcgaacaa tgagccccga aaccatctat
     1981 gtcgcaccgt tgagcctggt cgagaccacg gtcgccgacg ccaatgtcag ccacttggtg
     2041 actctgatca acggcgagac cctgatcaac acgccgccca gcatcgtgcc cgaccggcat
     2101 ctcaggctcg ccatgaacga catctgcgag ccgcagcccg ggctcgtgct tccctgcgag
     2161 acgcatgtgg ccgatctcat cggcttcgcg cgcgattggg accggcaggc gccgctgctc
     2221 atccattgct gggccggcat cagccgctcg acggccgccg ccttcatctc gctctgcgcg
     2281 ctcaatccgg aaggctccga gctcgagctc gcccgggtgc tccgccgcgc ctcgccgacc
     2341 gcctatccca atcggctgct ggtcgcgctt gccgacgaga tcctgacgcg cgacggacgc
     2401 atgatcaacg cggtcgagga gatcgggcgc ggcaagatcg ccgaggaggc ggaggtgttc
     2461 gccctgcccg cgctccttgc tgcatagacg cctcgatggc tgagccaagg cgcaaagcga
     2521 cgccggcgca ggagacgcgg aacgacgatc cgcaaatcga gcttgggctc aatgccgcca
     2581 tcgtcaccgt gcgcgaaggg cagccctata tcctcgttac gcgaccgggc actgcggggt
     2641 ccgacgcctg ggacgcgctt ccgttcggtc cattcgcccc gcgcgcgcac cgtacgctcg
     2701 agattggctt gcgcgaatgg gtgaaacgcc agaccgggct cgcgctcggt tatgtggaac
     2761 agctctacac attcggcgac aggggccgcc acgccgagcc cggcggagcg catacgctct
     2821 cggtcggcta tctcgcactg acgcgtggcg cgggcgatga gctgctcggc tcgcattggt
     2881 cgccttggta tgcctatttc ccgtgggagg attggcgcaa aggcaagccg cccatcctgt
     2941 ccagccagat cgagccgaag ctgaaggaat gggccgaatt gccgcccctc cccgacgagc
     3001 cggtacgccc gctcagacgc tccgagcgcc tcaggacgtc gttcggcatc ggcgggagct
     3061 gggacgagga aaaggtgctc gatcgctacg agctgctgta tgaggccggt ctcgtcgcgg
     3121 aggcggcgcg cgacgaccgt cccgccgcgg cgcaatggag cgatctgcct gatctcgggc
     3181 gccctatggc gttcgaccac cgccgcatcc tcgccacggc gctcgggcgc ttgcgtggca
     3241 agctgaaata ccggcccgtt gtgttcgagc tg
//
LOCUS       DATNAE010000077         2822 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3091815, whole genome shotgun sequence.
ACCESSION   DATNAE010000077 DATNAE010000000
VERSION     DATNAE010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3091815"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VN518_01585"
     CDS             complement(<1..321)
                     /locus_tag="VN518_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HXE03295.1"
                     /translation="MEMHQVRYFLAVARALNFTRAAEECHVAQPSLTRAIRQLEGELG
                     GDLFRRERPQAQLTELGQRMLPLLKQCYESALSARLLASAIKKGEVGSLRLALSRTID
                     PSLVT"
     gene            739..1563
                     /locus_tag="VN518_01590"
     CDS             739..1563
                     /locus_tag="VN518_01590"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HXE03296.1"
                     /translation="MNIAMTERRGSTAQLLKDKVSLVTGSTSGIGLGIARALAASGSA
                     VVLNGFGKAEEIKATQAAIVADFGVKVAYSGADMTNPDSIAEMITMTLDSFGRLDVLV
                     NNAGIQHVAPLQEFPVAKWDAILAINLSSAFHTTRLALPSMVANKWGRIINLASAHGL
                     VGSPFKSAYVAAKHGMLGLTKVTALETAEQGITCNAICPGYVYTPLVEAQIEGQAKAH
                     GIPREQVIREVLLAQQPNKRFATTDELGGIAVFLCSDAAASITGAAIPVDGGWTAH"
     gene            1593..2819
                     /locus_tag="VN518_01595"
     CDS             1593..2819
                     /locus_tag="VN518_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HXE03297.1"
                     /translation="MSKTKTLTPLDAVNEIRNKNNGQVVLVLQGGGALGAYQCGVYQA
                     LHEAGVEPDWIIGTSIGAINAGIIAGNRPEDRLARLKEFWFRVERANFWGSFPAWAGV
                     AEALASWMTLTKGISGFFEPHTRAFLGAHVHLGADHAGYYSTEPLRKTLEELVDFDLI
                     NRGHPRLTVGAAHVRSSMMHYFDSKEMKLSPSHVMASGALPPAFPAVRIDGELYWDGG
                     ILSNTPTERIFDDSPRRNSLIFAVHLWHPIGDEPETIWQILNRHKDIQYSSRIASHIV
                     RQRETHKLRHVITQLLEYVPEEEKNDPLYRELAGYHCDTRMHVVRLLSPKLDNETHTK
                     DIDFSPSGIRKRWEAGYADTMRALWRQPWIGEFGLLDGVILHEPEPGAEPAAEVEPSK
                     PNPDTSDRDPRKLAAE"
BASE COUNT          524 a          911 c          902 g          485 t
ORIGIN      
        1 ggtcaccagc gagggatcga tggtgcgcga cagggccagc cgcagcgagc cgacctcgcc
       61 tttcttgatg gcggaagcca gcaagcgggc gctgagtgcg ctttcgtagc attgcttcaa
      121 cagcggcagc atgcgctggc cgagctcggt gagctgggcc tgcggccgct cgcgccggaa
      181 cagatcgccg ccgagctcgc cctcgagctg gcggatggcg cgggtaagtg aaggctgggc
      241 gacgtggcac tcctccgcgg cgcgggtgaa attgagagct cgcgccaccg cgaggaagta
      301 gcggacctga tgcatttcca tgggagatgc gctccgctgc ctaatctatg cgtgaacgag
      361 acaggatcgg ggcatgggag caaggtccgc gtttcagcgg tcggactgga cggtgtccag
      421 ccagtttcct caccctgcaa acgatcttcg acaaggaaag tccgggttcg cgccgttgtt
      481 gtgagcggag ttgtacgctt gtgaccggtt tcccgtcgag atgatttgat agccaagcgc
      541 gtcttttggg ctggcggaag gtcgcggggg ccctcgcgaa atcctatttt ctccagcctg
      601 ggcaagcctt taggcggcca tagcgcaacc ctatcgagtc cgtagccaaa gggcatttcc
      661 gctcagtcac cgggcctggg catgatcctc gccaacagaa gccttggcgt caccacgccg
      721 atcgaggagt agccggccat gaacatcgcc atgacagagc gcaggggatc cacggcgcaa
      781 ctcctcaagg acaaggtgtc tttggtgacc gggtcgacca gcggcattgg gcttgggatc
      841 gcgcgcgcgc tggccgcttc gggctctgcg gtggttctca acggcttcgg caaggcggaa
      901 gagatcaagg cgacccaagc cgcgatcgtc gccgatttcg gcgtgaaggt cgcctattcc
      961 ggagcggaca tgaccaaccc ggactcgatc gccgagatga tcacgatgac cctcgactcc
     1021 ttcggcaggc tcgacgtcct ggtcaacaat gcgggcatcc agcacgttgc gcccttgcag
     1081 gaattcccgg tggccaaatg ggacgccatc ctcgccatca atctcagctc ggcctttcac
     1141 accacccggc tcgcgctgcc gtccatggtc gccaataaat ggggccgcat catcaatctc
     1201 gcctcggcgc acgggctggt cggctcgccc ttcaagtcgg cctatgtggc ggccaagcac
     1261 ggcatgctcg gcttgaccaa ggtcacggcg ctggagacgg ccgagcaggg catcacctgc
     1321 aacgcgatct gccccggcta cgtctacacg ccgctggtcg aggcgcaaat cgaggggcag
     1381 gccaaggcgc acggcatccc gcgcgagcaa gtgatccgcg aggtgttgct ggcgcagcag
     1441 ccgaacaagc gtttcgccac caccgacgag ctcggcggca tcgcggtgtt cctctgctcg
     1501 gacgccgccg cctcgatcac cggagccgcg atcccggtcg atggcggctg gacggcgcat
     1561 tgagcgaaaa gacaacgagc cgggaggcaa ccatgtccaa gaccaagacg ctgacgccgc
     1621 tcgacgcggt caacgagatt cgcaacaaga ataacggcca ggtcgtgctc gtgctgcaag
     1681 gcggcggggc gctcggcgcc tatcagtgcg gcgtctatca ggcgctgcac gaggccggcg
     1741 tcgagccgga ctggatcatc ggcacctcga ttggcgccat caatgccggc atcatcgccg
     1801 gcaataggcc ggaggaccgt ctcgccagac ttaaggagtt ttggttcagg gtggagcgcg
     1861 cgaatttctg gggttctttt cccgcctggg cgggtgtcgc cgaagcgctt gcatcatgga
     1921 tgacgctgac caagggcatc tccgggttct tcgagccgca tacgcgcgcc ttcctcggcg
     1981 cccacgtgca tctcggcgcc gaccacgccg gctattactc gaccgagccg ttgcgcaaga
     2041 cgctcgagga actggtcgat ttcgatctca tcaatcgtgg tcatccgcgg ctgacggtgg
     2101 gcgccgcgca tgtgcgctcg agcatgatgc attacttcga ttccaaggag atgaagctgt
     2161 cgccgagcca cgtgatggcc tcgggcgcgc tgcctccggc ctttcccgcg gtgcgcatcg
     2221 acggcgagct ttactgggac ggcggcatcc tctccaacac cccgaccgag cgcatcttcg
     2281 acgacagccc gcgccgcaac tcgctgatct tcgccgtgca tctctggcat ccgatcggcg
     2341 acgagccgga gacgatctgg cagatcctca accgtcacaa ggacatccaa tattcgagcc
     2401 ggattgccag ccacatcgtt cgccagcgcg agacgcataa gctccgccac gtcatcaccc
     2461 agctgctcga atatgtgccc gaggaggaaa agaacgatcc gctctatcgg gaacttgccg
     2521 gctatcattg cgacacgcgc atgcacgtgg tgcggctgct gagcccgaaa ctcgacaacg
     2581 agacccacac caaagacatc gatttctcgc cctcgggcat ccgcaaacgc tgggaggccg
     2641 gctacgctga tacgatgcgc gcgctgtggc gccagccctg gatcggcgag ttcgggctgc
     2701 tcgacggcgt catcctgcat gagccggagc ctggcgccga gccggctgcc gaggtcgagc
     2761 caagcaagcc aaatcccgac acgtccgacc gcgacccgcg caagctcgcc gcggagtgaa
     2821 gg
//
LOCUS       DATNAE010000078         2785 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2491511, whole genome shotgun sequence.
ACCESSION   DATNAE010000078 DATNAE010000000
VERSION     DATNAE010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2491511"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VN518_01600"
     CDS             complement(<1..282)
                     /locus_tag="VN518_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03298.1"
                     /translation="MAKAKYHKSQRVFVKPVGTWAYVEAVVPKWIKGCEEPIKITYDC
                     GMGREFAQEELEEESAALQTTAVNGLGGAWRVVRGHNRWKSAEQCGHHPH"
     gene            405..812
                     /locus_tag="VN518_01605"
     CDS             405..812
                     /locus_tag="VN518_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03299.1"
                     /translation="MSPEQNAEIAAPKLFLPSWRAGTLLAALGVVALSAALYLRYAII
                     QNSTIGVACEAGEVSFLCRLRLGVILMFAQDCFGWVAMIAACVQLWRPNIVAFGTGLI
                     FALMGLVLYNTRASALAIALLVLSLARPSPAKS"
     gene            complement(778..2385)
                     /locus_tag="VN518_01610"
     CDS             complement(778..2385)
                     /locus_tag="VN518_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-glucan synthase"
                     /protein_id="HXE03300.1"
                     /translation="MLRSLGLFTLIACAIALAWYGLGRPVAMPPSPLGPGEKLTCISY
                     APFHGDQAPFTGDLRISDAQIAEDLRRLSALTACVRTYSAKGAQGRITRLAEAQGLTV
                     LQGIWLGRNRADNRREIEAGLQLARRHPGTVKALIVGNETLLRGELAPATVGTYLAEV
                     KRRSGLPVTYADVWEFWLKSPELASAADFVTIHILPYWEDDPVSAKDAVAHVREVRDR
                     VQATFPGKEIWIGEVGWPSAGRMREGALPSPANQARVLSDVVAAAKQDGWKVNLIEAF
                     DQPWKRLMEGTVGGYWGVYGDQGRKPKFQFGQSISNHPDWRLKAGLGIGAAFFVFVAF
                     WLGSRKAPTRARSWQLDAAASIIALGVGLVFGLAVTNLPMEGEVASDRVRSVAMLVLA
                     LVVPAASAYALARGDRLAGLAEALDPARWHRHDLVEIVLAALLAATLVAALHVALGLV
                     FDPRYKDFPFAALAGPVIALAVLGLATARAPVKPGAAEAAAAIVLTASALFVIVNEGI
                     ANWQALVLAGLLLLLALTTLQAKAVPG"
     gene            2481..>2785
                     /locus_tag="VN518_01615"
     CDS             2481..>2785
                     /locus_tag="VN518_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03301.1"
                     /translation="MSPAVRPPKSRGLMHKMTYSCVGHLSMLRCNLSPATALIACGIF
                     VVSSCAAFAQQSGGDQKPPEQAQGEQKSDDKTANDQKAAEQAAQQEAAKAIEEYKEAA
                     "
BASE COUNT          510 a          902 c          904 g          469 t
ORIGIN      
        1 gtgaggatgg tgcccgcact gctcggcgct cttccagcga ttgtgaccgc gcacgacgcg
       61 ccaagcgccg ccgaggccgt tcaccgcggt agtctggagg gccgcagact cttcctccag
      121 ctcctcttgc gcgaactcgc ggcccatgcc gcaatcgtag gtaatcttga tcggctcctc
      181 gcagcctttt atccatttcg gcaccaccgc ctcgacgtag gcccaggtgc cgaccggctt
      241 gacgaaaacg cgctgcgatt tgtggtactt ggcttttgcc atgttgactg tccccaactc
      301 gggagccgag gctcctaagc cttggattag atgagaaatg ttaaaatttg gacccgcgtc
      361 ggtcttggcg cgggcgcatc gcttcgctta agacacgctt caccatgagc cccgagcaaa
      421 acgccgaaat cgccgcgccc aagctgttcc tgccgtcgtg gcgggcgggg actttgcttg
      481 ccgcgctcgg cgtggtggcg ctcagcgcgg cgctctatct ccgctatgcg atcatccaga
      541 attcgacgat cggggttgct tgcgaggcgg gcgaggtcag ttttctctgc cgactgcgcc
      601 tcggggtgat cttgatgttc gcgcaggact gcttcggctg ggtggcgatg atcgccgctt
      661 gcgtccagct gtggcggccg aacatcgtgg ccttcggcac gggacttatc ttcgcgctga
      721 tggggctcgt gctttacaat acgcgcgcct cggcgctggc gatcgcgctg ctcgtgctca
      781 gcctggcacg gccttcgcct gcaaagtcgt aagcgcgagc agcaagagca gccccgcgag
      841 aacaagcgcc tgccagttgg caatcccctc gttgacgatc acgaacagcg ccgaggcggt
      901 cagcacgatc gccgccgcgg cctcggccgc accgggcttg accggcgcgc gcgcggtcgc
      961 aaggccgagc accgcgaggg cgatgaccgg gcccgcgagc gcggcgaaag ggaaatcctt
     1021 gtagcgtggg tcgaacacca gcccgagtgc cacatgcagg gcggcgacca gcgtcgcggc
     1081 aagcagcgcc gcgagcacga tctcgacgag gtcgtggcgg tgccagcgcg ccggatcgag
     1141 cgcctcggca aggccggcaa gacgatcgcc gcgcgcgagc gcataggcgc tggccgcggg
     1201 aaccacgaga gccagaacaa gcatggcgac cgagcgcacc ctgtcgctcg ccacttcacc
     1261 ctccatcggc aggttggtga cggccaggcc gaagacgagg ccaacgccca gcgcgatgat
     1321 cgaagcggcc gcgtcaagct gccaggagcg cgcgcgtgtg ggggccttgc gggatccaag
     1381 ccaaaaggcc acaaaaacga aaaaagcggc gccgatgccg agccccgcct tgagccgcca
     1441 gtccgggtgg ttggatatgc tctgtccgaa ctggaatttc ggttttcggc cctggtcgcc
     1501 gtaaaccccc caatagccgc cgaccgtccc ttccatcagg cgcttccaag gctggtcgaa
     1561 ggcctcgatg aggttcacct tccacccgtc ttgtttggcc gccgcaacga cgtcgctcag
     1621 gacgcgcgcc tgattggccg gggaggggag agccccttcg cgcatccgtc cggcactggg
     1681 ccagcccact tcgccgatcc aaatctcctt gccggggaag gtcgcctgca ctctgtcgcg
     1741 tacctcgcgc acatgggcga cggcatcctt ggcgctcacc ggatcgtcct cccaataggg
     1801 aaggatatgg atggtgacga agtcggccgc cgaggcgagc tcgggcgact tgagccaaaa
     1861 ttcccagaca tcggcatagg tcaccggcag gccagagcgc cgcttgacct ccgccagata
     1921 ggtcccgacc gtcgcgggcg caagctcgcc cctcaacagc gtctcattgc cgacgatcag
     1981 cgccttgacg gtcccgggat ggcggcgggc gagctgcaga cccgcttcga tctcgcgacg
     2041 attgtcggca cggttccggc cgagccagat cccttgcagc acggtaaggc cctgggcctc
     2101 ggcaagccga gtgatccgcc cctgcgctcc cttggccgag taggtccgga cgcaagcggt
     2161 aagcgccgaa agccgccgca aatcctcggc gatctgcgcg tcggaaatgc ggagatcccc
     2221 ggtgaatggc gcttggtcgc catggaacgg cgcgtaggag atgcacgtta gcttctcgcc
     2281 cggaccgagg ggcgagggag gcatggcgac cggccgtccc aagccatacc aggcgagggc
     2341 gatcgcgcag gcgattaggg taaacaggcc aagggagcga agcatggtga gggttgagtc
     2401 gagggggatg cgacgaatag cagcttcgca ggtctccttg aaccaaatcc tctggtttgt
     2461 gcgcttgcga agtgataaac atgagtcctg ccgtcaggcc gccaaaaagt cgcggtctga
     2521 tgcataaaat gacctattcc tgcgttgggc acctctcgat gctgcgctgc aatctctcgc
     2581 cagccaccgc tctcatagcg tgtggcattt ttgttgtgtc ctcctgcgcg gctttcgccc
     2641 agcaatcggg cggcgaccag aagcccccgg aacaggcgca aggcgaacag aaatccgacg
     2701 acaagactgc caacgatcag aaggcggccg agcaggctgc gcagcaggaa gctgccaagg
     2761 ccatcgagga atataaagaa gcggc
//
LOCUS       DATNAE010000079         2576 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3998392, whole genome shotgun sequence.
ACCESSION   DATNAE010000079 DATNAE010000000
VERSION     DATNAE010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3998392"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="VN518_01620"
     CDS             complement(<1..290)
                     /locus_tag="VN518_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009467187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HXE03302.1"
                     /translation="MDVAIIDYGSGNLHSAAKAFERAARESGSPLKVKVTSDPEDVRA
                     AERIVLPGVGTFADCKQGLVAIPGMIEALEESVRRRGKPFLGICVGLQLMAER"
     gene            complement(297..752)
                     /locus_tag="VN518_01625"
     CDS             complement(297..752)
                     /locus_tag="VN518_01625"
                     /inference="COORDINATES: protein motif:HMM:NF022400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HXE03303.1"
                     /translation="MQRADRLAFVKDGFSWPALFVPLLWLIYHRMWIELIVLGLIYLA
                     LQLAFGTDTQGQALTLWAGLAVGVLFAFEANDLRAAALERRGYRLAGVASGRDRIEAE
                     RSFFTAWLPQQASAARAPARGSESRRETEAPPAVQRGGSEEVIGLFPQA"
     gene            complement(847..1461)
                     /gene="hisB"
                     /locus_tag="VN518_01630"
     CDS             complement(847..1461)
                     /gene="hisB"
                     /locus_tag="VN518_01630"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015933823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HXE03304.1"
                     /translation="MQKRHKDTEARRATVERKTKETAISASVDLDGTGAYEVSTGVGF
                     LDHMIEQLARHSLIDITLKAKGDLHIDHHHTTEDAGIVLGQAVARALGDKTGIARYGF
                     AYLPMDETLTRVALDVSGRPFLVWKVDFTRSKIGEMDTELFREWFQAFAQHAGLTLHV
                     ETLYGENSHHIAETCYKALARALRQAVAIDERQGARVPSTKGQL"
     gene            1606..2169
                     /gene="hslV"
                     /locus_tag="VN518_01635"
     CDS             1606..2169
                     /gene="hslV"
                     /locus_tag="VN518_01635"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009466537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HXE03305.1"
                     /translation="MGSSPASPDTWHGTTILTVRKGGKVVIAGDGQVTLGQTIIKGTA
                     RKVRRVGTGNVIAGFAGSTADAFTLFERLETKLERFPDQLKRACVELAKDWRTDRYLR
                     RLEAMMIVADADESLVLTGTGDVLEPDDAVMGIGSGGSYALAAARALLDSDLDAETIA
                     RRAMAIAATICVYTNDQVVVETLDAAK"
     gene            2179..>2576
                     /locus_tag="VN518_01640"
     CDS             2179..>2576
                     /locus_tag="VN518_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012759584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXE03306.1"
                     /translation="MTSFSPREIVSELDRHIVGQRDAKRAVAIALRNRWRRQQLDEEM
                     REEVLPKNILMIGPTGVGKTEISRRLAKLAGAPFIKVEATKFTEVGYVGRDVDQIVRD
                     LLEAGIGLVRDVKRKEVAAKAQLNAEERVI"
BASE COUNT          446 a          856 c          861 g          413 t
ORIGIN      
        1 cgctcggcca tcagttgcag cccgacgcag atgccgagaa acggcttgcc gcgccgccgg
       61 acgctctcct cgagcgcttc gatcatgccg ggaatggcga cgagcccctg cttgcagtcg
      121 gcaaaggtgc cgacgcccgg cagcacgatc cgctcggccg cgcgcacgtc ttccgggtcg
      181 gaagtcacct tgaccttgag cggcgaaccg ctttcccgcg ccgcgcgctc gaaggccttg
      241 gcggcggaat gcaggttccc cgaaccgtaa tcgatgatgg cgacgtccat gtcggatcaa
      301 gcctgcggga acagcccgat gacctcttcg ctcccgccgc gctgcaccgc cggcggtgct
      361 tccgtctcgc ggcgggactc gctgccgcgc gcgggggccc gggcggcgct cgcttgctgc
      421 ggcagccagg cggtgaagaa cgagcgctcg gcttcgatcc tgtcgcgccc gctggcgacg
      481 cccgcgagcc gataaccgcg ccgttcgagc gcggccgcgc ggagatcgtt ggcctcgaag
      541 gcaaacagca cgccgacggc caggcccgcc cacagcgtca gcgcctggcc ttgcgtatcg
      601 gtgccgaacg caagttgcag agccaggtag atgagaccga gcacgatcag ctcgatccac
      661 atgcggtggt agatcagcca gagcagcggc acgaacaggg caggccagga gaaaccgtcc
      721 ttgacgaagg cgagcctgtc ggcccgctgc ataaggtcgg gcgcctccgc gggcggatca
      781 taaacggaat agacggtcac tctaagcgct cacgctgttc tgcccgctcg gaagcaggca
      841 aggcctctaa agctggccct tggtcgaggg cacgcgggcc ccttggcgct cgtcgatggc
      901 gacggcctgg cgaagcgccc gggcgagcgc cttatagcag gtctcggcga tatggtgact
      961 attctcgccg taaagcgtct cgacatgcag cgtcagcccg gcatgctggg cgaaggcctg
     1021 gaaccactcg cggaacagct cggtatccat ctcgccgatc ttggagcggg tgaaatcgac
     1081 cttccagacc aggaacggcc gccccgagac gtcgagcgcg acgcgcgtca gcgtctcgtc
     1141 catcggcaga taggcaaagc cgtagcgggc gatgcccgtc ttgtcgccga gcgcgcgtgc
     1201 cacggcctgg ccgagcacga tgccggcatc ttcggtggtg tggtggtggt cgatatgcag
     1261 atcgcccttg gccttgagcg tgatgtcgat cagcgaatga cgcgcaagct gctcgatcat
     1321 gtgatcgagg aagccgacgc cggtcgagac ctcgtaggcg cccgtgccgt cgagatcgac
     1381 ggaggcggag atcgccgtct ccttggtctt gcgttcgacc gtcgcccggc gggcttcggt
     1441 gtctttgtgt cgcttctgca tggtaagaga ctatctcaag gctcttagca attcgcttcc
     1501 gagcgccagc cagcttatac ggcagcgggc atgcgagtgc tatcagagcg ttaacgccgg
     1561 ccgagcttga cgaatagggc ctaactcccg atatcgcgtg gcacgatggg ttcctctccc
     1621 gcttcccccg acacctggca cggcaccacg attctgaccg tccgcaaggg cggcaaggtg
     1681 gtgatcgccg gcgacggcca ggtcaccctc ggccagacca tcatcaaggg cacggcgcgc
     1741 aaggtgcggc gcgtcggcac aggcaatgtg atcgccggct tcgccggatc gaccgccgac
     1801 gccttcacgc tgttcgagcg gctggagacc aagctcgagc gctttcccga ccagttgaag
     1861 cgcgcctgcg tcgagctcgc caaggactgg cgcaccgacc gttatctgcg ccggctggag
     1921 gcgatgatga tcgtggccga cgccgacgag agcctggtct tgaccggcac cggcgacgtg
     1981 ctcgagcccg acgacgcggt gatgggcatc ggctccggcg gatcctatgc gctcgccgcc
     2041 gcgcgggcgc tgctcgactc ggacctcgat gccgagacga ttgcgcgcag ggccatggct
     2101 attgccgcca cgatttgcgt ttacaccaat gaccaggtgg tcgtcgaaac cctggacgcg
     2161 gccaagtagt aggagtcaat gaccagcttc tccccacgcg agatcgtctc cgagctcgac
     2221 cgccacatcg tcggccagcg cgacgccaag cgcgccgtcg ccatcgcctt gcgcaaccgc
     2281 tggcggcgcc agcagctcga cgaggagatg cgcgaggagg tgctgccgaa gaacattttg
     2341 atgatcggcc ccaccggcgt cggcaagacg gagatctcgc gtcgcctcgc caagcttgcc
     2401 ggcgccccct tcatcaaggt cgaagccacc aaattcaccg aggtcggcta tgtcggccgc
     2461 gacgtcgacc agatcgtgcg cgatctgctc gaggcgggca tcggcctggt gcgcgacgtg
     2521 aagcggaaag aggtggcggc caaggcgcag ctcaacgccg aggagcgggt gatcga
//
LOCUS       DATNAE010000080         8049 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2796853, whole genome shotgun sequence.
ACCESSION   DATNAE010000080 DATNAE010000000
VERSION     DATNAE010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 8049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8049
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2796853"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1227
                     /locus_tag="VN518_01645"
     CDS             <1..1227
                     /locus_tag="VN518_01645"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HXE03307.1"
                     /translation="NTMLRPTRYARPSDFIKYRALEAASACFAQEPVAAPVVVSFEAT
                     LNPAGVEQPACTLCGDCCSGCNVGAKNTIALTYLPDAKAHGAEIFTELTVSHVAREGR
                     HWRVHFVPTEEPDAPLRSVEAKTVVLSAGTLGSTEILLRSRDEGLPVSDRLGERFSAN
                     GDIIAFALGGKGRVNSIGVGYPPKFVGDAIGACVTGEIELNDDGRLDHSMFIQEGVLP
                     SALAPLLPVFFISGGRLLGAAQSLIKGVYHGPLQSLYTFFVVSHDEARGRIRLENGKA
                     QIDWPNVADQPVYARVDAALSKAAQSVGARYIKSPLAATTMGTKPATAHPLGGCGMGD
                     DASSGVVDHKCRVFDGKGHVHAGLYVCDGAIIPRSIGVNPLLTITALTERAMIHFARD
                     HNLGFDDAPRRLVEVS"
     gene            complement(1230..3644)
                     /gene="pheT"
                     /locus_tag="VN518_01650"
     CDS             complement(1230..3644)
                     /gene="pheT"
                     /locus_tag="VN518_01650"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HXE03308.1"
                     /translation="MKFTLSWLKDHLDTRASVAEIADALNRIGLEVEEVFDPATALAP
                     FRVAKVVKCEKHPNADKLSVCEVDTGAERLQVVCGAPNAREGLKGVFAPVGSYVPGID
                     LTLTKAKIRGVESSGMLLSERELELSDEHTGIIELAPDAKIGRPAAKALGLDDAVIEI
                     AITPNRPDCLGVRGVARDLAAAGLGKLKPDTVKPVKGSFPNPIPIKLDFDKDTASACP
                     VFAGRVLRGVKNGPSPEWLQRRLKAIGLRPINALVDITNYISYDRGRPLHVYDADGLK
                     GAIRARLGRKGESFKALDGKTYEVDSDMCVIADDSGVLGLGGVIGGEETGSTDATTNV
                     FIESAYFDPKRTARTGRKLNIQSDARFRFERGADPGFVVPGLELATRMVLDVCGGEAS
                     KMEIAGKPPKPNAPFAFDPKLVKRLSGLDLKGGEIRRLLGALGVELKGTASKLKAAPP
                     SWRPDISVPVDLVEEVVRLVGVDRVPATPMPREAGVARPVLTEMQFRTRRVRRVLAAR
                     GLVEAVTWSFIPPDQARLFGGGAAELTLSNPISTELAQMRPGLLPGLVTAAQRNRDRG
                     FADGALFELGQAYRGPLPEDQFIAAAGVRFGHSALAGSGRDWSGEAPPADVFAVKADA
                     VAALAALGIDQGTLSVTREAPSWFHPGRSGALKLGPKVTLGTFGELHPDVLTNLGVDA
                     PLAGFELYLDAIPAPKRKSLTKPALDASDLQAVKRDFAFLLDEDVAAADVLHAAQVAD
                     RALITNVGVFDVFTGQGVPQGKKSLAIEVTLQPREKTLTDTEIEAVAAKIVAAVTKAT
                     GGELRG"
     gene            complement(3648..4754)
                     /gene="pheS"
                     /locus_tag="VN518_01655"
     CDS             complement(3648..4754)
                     /gene="pheS"
                     /locus_tag="VN518_01655"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006934061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HXE03309.1"
                     /translation="MAKTPTAQTASLEGIEGELLAAIAKAGDLGALEEVRVAALGKKG
                     RVSELMQTLGRMAPEERKSFGQAVNQLKERVGEALDARRETLGRAALSARLAAERADV
                     TLPVREGPLAEGRIHPVSQVADEITEIFADLGFSIAEGPDIETDFNNFTALNIPPEHP
                     ARQDHDTFYFAESADGTRPVLRTHTSPVQIRTMKKQKPPLRIIAPGRVYRSDSDQTHT
                     PMFHQVEGLAIDEETHLGHLKWVLEEFCKAFFEVPQVKMRFRASHFPFTEPSMEVDIG
                     YAKVGDEIRIGEGDQWLEILGCGMVHPNVLKNVGLDPARYQGFAFGLGIDRIAMLKYG
                     MPDLRAFFSADLRWLKHYGFASYAVPTLAGGLGQ"
     gene            complement(4768..5127)
                     /gene="rplT"
                     /locus_tag="VN518_01660"
     CDS             complement(4768..5127)
                     /gene="rplT"
                     /locus_tag="VN518_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HXE03310.1"
                     /translation="MSRVKRGVTSHARHRKVLKQAKGYHGRRKNTIRVAKQAVEKAGQ
                     YAYRDRKVRKRTFRALWIQRINAAARLHGLTYGRFIDGLNKAGIELDRKVLADIAVHE
                     PATFQSLVEAASAAAKA"
     gene            complement(5158..5361)
                     /gene="rpmI"
                     /locus_tag="VN518_01665"
     CDS             complement(5158..5361)
                     /gene="rpmI"
                     /locus_tag="VN518_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HXE03311.1"
                     /translation="MPKMKTKSGAKKRFKVTATGKVKCQASRKRHGMIQRPTKFIRNA
                     RGTMVLSDEDAKIVKKNFLPYGR"
     gene            complement(5528..6049)
                     /gene="infC"
                     /locus_tag="VN518_01670"
     CDS             complement(5528..6049)
                     /gene="infC"
                     /locus_tag="VN518_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HXE03312.1"
                     /translation="MKLTPDRGGPRINEGIDTPNIQLIDATGNNVGVVGIADALARAA
                     EAGLDLVEISPNSEPPVCKILDFGKYKYQAQKKAAEARKKQRTVEIKEIKMRPNIDIH
                     DYDVKMRSIVRFFEEGDKVKVTLRFRGREMAHMELGAKLLDRVKQDTSTLAKVESEPR
                     LEGRQMVMVLAPR"
     gene            6264..6632
                     /locus_tag="VN518_01675"
     CDS             6264..6632
                     /locus_tag="VN518_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03313.1"
                     /translation="MRPSLLLAVVALACWGMPFPVSAQFTRCIFISGTADALVKPNAV
                     EASQKSLREAIDKWKADNGVTAPVSEYAQKPRPVPYWRSEVDPSLFLPPDVVSDTAYT
                     LCWKGVVSPVVCTTGAKLCW"
     gene            complement(6591..7814)
                     /locus_tag="VN518_01680"
     CDS             complement(6591..7814)
                     /locus_tag="VN518_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020083992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXE03314.1"
                     /translation="MVTILQVVPRLDAGGSELATIEIAQALSRAGASALVATEGGRMA
                     DEVGKAGGEIVTLPVASKSPFTILANIRRLTKLIKDRKVDLVHARSRAPAWSALLAAR
                     RTGKPFVTTYHGAYGEVGPFKAAYNSVMAKGDRVIANSRYTASVIEARAPRAKDRIRV
                     IYRGVDRYVFDPAAVDAQSVEKLRAAWGVPQGTKIVLHAARLTSIKGQRDLIAAAALA
                     NAEGALRNAVVILAGDAPGRDAYREELLGLIARHGLGDKVRLVGHCREMPAAYLASQV
                     AVTTSTVPETFGRTSAEAQAMGCPVIVPDLGALPETIVAQGQGQAGFTGWLFPPRDIA
                     SLASRIGRALGLKAAERAAIGVRAREHVAANFTLAQMQEATLAVYDELLGSDLAEKFK
                     RASPAELGAGGTNDR"
BASE COUNT         1360 a         2617 c         2563 g         1509 t
ORIGIN      
        1 aacaccatgc tgcgcccaac ccgctacgca cgcccctcag acttcatcaa atatcgcgcc
       61 cttgaggcgg cgagcgcctg cttcgcgcaa gaaccggttg cggctcccgt cgtagtcagc
      121 ttcgaggcga ccctcaatcc cgccggcgtc gagcaaccgg cctgcacgct ctgcggagat
      181 tgctgctcgg gctgtaatgt cggcgccaag aataccatcg cgctcaccta tcttcccgac
      241 gccaaggcgc atggcgccga gatcttcacc gagctcaccg tgagccatgt cgcaagggaa
      301 gggcgccatt ggcgcgtcca tttcgtgccg accgaggaac ccgatgcgcc gttgcgctcg
      361 gtcgaagcca agacggtggt gctctcagcc ggcacgctcg gctcgaccga gatcctgctc
      421 cgctcgcgcg acgaggggct ccccgtgtcc gaccggcttg gcgagcgctt ctcagcgaac
      481 ggcgacatca tcgcctttgc tcttggcggc aaggggcggg tgaacagcat cggcgtcggc
      541 tacccgccga aattcgtggg cgacgccatc ggcgcctgcg tcaccggcga gatcgagctc
      601 aatgacgatg gccgcctcga ccactcgatg ttcatccagg aaggcgtgtt gccctcggcg
      661 ctcgcgccgc tcctcccggt gttcttcatt tcgggcggca ggctgctcgg cgccgcccag
      721 agcctgatca agggcgtcta tcacggtccg ctgcaaagcc tttacacctt cttcgtggtc
      781 tcccatgacg aggcacgcgg ccgcatccgc cttgagaacg gcaaggcgca gatcgattgg
      841 ccgaacgtcg ccgatcagcc tgtctatgcc cgcgtcgatg ccgccttgag caaggcggct
      901 caatcggtcg gcgcgcgcta catcaagagc ccgctcgccg cgaccaccat gggcaccaag
      961 ccggcgaccg cccatccgct tggcggctgc ggcatgggcg atgacgcctc gagcggcgtg
     1021 gtcgatcaca aatgccgggt gttcgatggc aaaggccacg tgcatgcggg gctttacgtg
     1081 tgcgacggcg ccatcatccc gcgctcgatc ggcgtcaatc cgctgctcac catcaccgcg
     1141 ctcaccgagc gggccatgat ccacttcgcc cgcgaccaca atctcggctt cgacgatgcg
     1201 ccgcgtcgtc tggtcgaggt gagttgaaac tagccgcgca actcgccgcc ggtggccttg
     1261 gtgacggcgg cgacgatctt cgcagcgacc gcctcgatct cggtgtcggt cagggtcttc
     1321 tcccgcggct gcagcgttac ctcgatggca agcgactttt ttccttgcgg cacgccctgt
     1381 ccggtgaaca cgtcgaacac gcccacattg gtgattagcg cccggtcggc gacttgcgca
     1441 gcgtgcagca cgtcggcggc ggcaacatct tcatcgagca ggaaggcgaa gtcgcgcttc
     1501 acagcttgca ggtccgaggc gtcgagcgcc ggcttggtca gggatttgcg tttgggcgcc
     1561 ggaatggcat cgaggtaaag ctcgaaacca gcgagcggcg catccacgcc aagatttgtg
     1621 agcacgtcgg gatgaagctc gccgaaggtg ccgagcgtga ccttcggtcc gagcttgagc
     1681 gcgcccgagc gtcccggatg gaaccaggac ggcgcctccc gcgtcacgct gagggttccc
     1741 tgatcgatgc cgagggcggc aagcgccgcc accgcatcgg ccttgacagc gaacacatcc
     1801 gcgggcggag cctctcctga ccaatcacgg cctgagccgg caagcgcaga atggccgaag
     1861 cgcacgccgg ctgccgcgat gaactgatcc tcgggcagag gcccccgata ggcctgacca
     1921 agctcgaaca gcgccccgtc ggcgaagccg cggtcgcggt tccgctgtgc cgcagtgacg
     1981 aggccgggca ggaggccagg ccgcatttgg gcgagctcgg tcgagatcgg attgctgaga
     2041 gtgagttccg ccgcgccgcc gccgaacagt ctcgcctgat caggagggat gaacgaccag
     2101 gtcaccgcct cgaccagccc gcgcgcggca agcacacgcc gcacgcgccg cgtccggaac
     2161 tgcatctcgg tcagcaccgg acgcgccacg ccggcctcac gcggcatcgg cgtcgccgga
     2221 acccgatcga cgccgacgag ccgcaccacc tcctcgacga gatcgacagg cacggaaatg
     2281 tccggccgcc atgagggcgg cgcggccttg agcttcgatg ccgtgccttt cagctccacc
     2341 ccaagtgcgc cgagcaggcg tctgatctcc ccgcctttga ggtcgagccc actcaagcgc
     2401 ttgaccagct tcggatcgaa ggcgaacggc gcattgggct tcggcggctt gccggcgatt
     2461 tccatcttgc tggcctcgcc gccgcaaacg tcgagtacca tgcgcgtcgc aagctcgagc
     2521 ccaggcacga cgaagcccgg atcggcgccc cgctcgaagc gaaagcgcgc atcggactga
     2581 atattgagct tgcggccggt gcgcgccgta cgcttcggat cgaaataggc agactcgatg
     2641 aacacgttcg tggtggcgtc ggtcgagccg gtttcttcgc cgccgatgac gccgccaagg
     2701 ccgagtacgc cgctgtcgtc ggcgatgacg cacatgtcgg aatcgacctc ataggtcttg
     2761 ccgtcgagcg ccttgaagga ctcgcccttg cgtccgagcc gcgcgcggat cgcgcctttg
     2821 agcccgtcgg cgtcgtagac atgcagcggc ctgccgcggt cgtaggaaat gtagttggtg
     2881 atatcgacga gcgcattgat cggccgaagc ccgatcgcct tgaggcgccg ctgaagccac
     2941 tcagggcttg ggccgttctt cacgcctctg agcacgcgtc cggcaaagac cgggcaggcg
     3001 ctcgcggtgt ccttgtcgaa atcgagcttg atgggaattg gattggggaa gctgcctttg
     3061 accggcttta ccgtatccgg cttcagcttg ccgaggccgg cagcggcgag gtcgcgggcg
     3121 acgccgcgca cgccgaggca gtctggccgg tttggcgtga tggcgatctc aatcaccgca
     3181 tcgtcgaggc cgagagcctt cgccgcaggt ctgccgatct tggcgtcggg ggcaagctcg
     3241 atgatgccgg tatgttcgtc ggagagctcg agctcgcgct ccgacagaag catgccgctc
     3301 gactcaacgc ccctgatctt ggccttggta agcgtgagat cgatgccggg cacatagctg
     3361 ccgaccggcg cgaacacgcc cttgaggccc tcgcgcgcgt tcggcgcgcc gcagacgacc
     3421 tgcaggcgct cggctccggt atcaacctcg cagacagaga gcttgtcggc gtttggatgc
     3481 ttctcgcatt tgacaacctt cgccaccctg aacggcgcga gcgcggtcgc ggggtcgaac
     3541 acctcctcga cctcgagccc gatgcggttg agcgcgtcgg cgatctctgc cacgctcgcg
     3601 cgtgtgtcga gatgatcttt cagccaggac agcgtgaatt tcatgaccta ctgaccaagg
     3661 ccaccggcaa gggtcggcac tgcgtaagaa gcgaagccgt aatgcttgag ccagcgtaag
     3721 tcggccgaga agaaagcgcg caagtctggc atgccgtatt tcagcatggc gatacggtcg
     3781 atgcctaagc cgaaggcgaa gccctggtag cgcgccggat cgagcccgac attcttgagc
     3841 acgttcggat gcaccatgcc gcagccgaga atctcgagcc actggtcgcc ttcgccgatc
     3901 ctgatctcgt cgccgacttt ggcgtacccg atgtccactt ccatcgatgg ctcggtgaac
     3961 gggaagtggc tcgcgcggaa gcgcatcttc acttgcggta cttcgaagaa cgccttgcag
     4021 aattcctcga gcacccattt cagatgcccg agatgagtct cctcgtcgat ggcgagcccc
     4081 tcgacctggt ggaacatcgg cgtatgcgtc tggtcagagt cgctgcgata gacgcgtcca
     4141 ggcgcgatga tgcggagcgg cggcttctgc ttcttcatgg tgcggatctg caccgggctc
     4201 gtatgggtgc ggagcactgg ccgcgtgccg tccgcacttt ccgcgaaata gaaggtgtcg
     4261 tggtcctgcc gcgccggatg ctcgggcgga atgttgagcg cggtgaaatt attgaagtcg
     4321 gtctcgatgt ccggcccctc ggcgatggag aagcccaaat cggcgaagat ttcggtgatc
     4381 tcgtcggcaa cctggctcac cgggtggatg cggccttcgg cgagcggacc ttcacgcacc
     4441 gggagcgtaa cgtcggcccg ctcagccgcg agccgcgcgc tcaacgcagc gcggccaagc
     4501 gtctcgcgcc tggcgtccag cgcctctccg acgcgctctt tgagctgatt gaccgcttgg
     4561 ccgaagctct tgcgctcttc cggcgccatg cgcccgagcg tctgcatcag ttcggagacg
     4621 cggcccttct tgccgagcgc ggcgacgcgc acctcttcca acgcgccgag gtcacctgcc
     4681 ttggcgatcg cggcaagcag ctcgccctcg atcccctcga gcgaagcggt ctgagccgtg
     4741 ggcgtcttgg ccacaggggc ttaaagctca ggccttggcc gcagctgacg cggcctcgac
     4801 caggctctgg aaggttgccg gctcgtgcac ggcgatatcg gcaagcacct tgcggtcgag
     4861 ctcgatgccg gccttgttca ggccgtcgat gaaccggcca taggtgagcc catgcaggcg
     4921 cgcggcagca ttgatgcgct ggatccacag cgcccggaac gtgcgcttgc gcaccttgcg
     4981 gtcgcgatag gcgtactgcc cggccttctc taccgcctgc ttggcgacgc gaatggtgtt
     5041 cttgcggcgg ccgtgatagc ccttggcctg cttcagcacc ttgcggtggc gagcatggct
     5101 ggtaacgccg cgtttcacac gtgacattgg gtgctccttc gaagtcggcc ctgggctcta
     5161 tcggccgtag ggcagaaaat tcttcttcac gatcttggcg tcctcgtcgg agaggaccat
     5221 ggttccgcgc gcgttcctga taaacttggt cgggcgctga atcatgccat gccgcttgcg
     5281 cgaggcttgg cacttgacct tgcctgtggc cgtgaccttg aagcgcttct tggcaccgct
     5341 cttggtcttc atcttgggca tttggcttcc ttcttggcgg cccgccgtct caagttgaga
     5401 cggagccatg gtttatcgat ggttgaagca tggaagccgc cacggcatgc cccgccgggc
     5461 ggctccttaa gaacggcgcc ttatagagtg gattggcgcc ggaacgcaag ggtgagagcc
     5521 agaaatccta gcgtggcgcg agcaccatca ccatttgccg gccttcgagc cttggctccg
     5581 attccacctt ggcgagcgtc gaggtgtcct gcttcacccg gtcgagcagc ttggcgccga
     5641 gctccatatg cgccatctcg cggccgcgga agcgcaaagt gaccttcacc ttgtcgccct
     5701 cctcgaaaaa gcggacgatc gagcgcatct tcacgtcata gtcgtgaatg tcgatgttcg
     5761 gccgcatctt gatctccttg atctcgacgg tgcgctgctt cttgcgcgcc tcggcggcct
     5821 ttttctgcgc ctgatatttg tacttgccga aatccaggat cttgcacacc ggcggctcgg
     5881 aattgggcga gatctcgacg agatcgagcc ctgcctcggc ggcgcgcgcc aaggcatcgg
     5941 caatgccgac cacgccgaca ttgttaccgg tagcgtcgat gagttggatg ttcggagtgt
     6001 caatgccctc gttgatacgc gggccccccc tgtcgggagt gagtttcatc ggccgtcttg
     6061 cgatgtgtcg gtctcctttg ttgtggcacg tcctaaagcc gctcgcgggg aagcctgaat
     6121 cggcggtccc gggcgaacct agggtttcta taggatcggc aacctagctg acaagtcaag
     6181 gcgctgtgtt tacgtcgcac ggcctttgta cttttccggc aaatttgcca tgtttgcgat
     6241 gagacgttcg aaaagggaat tgaatgcgcc caagtctgct gctcgccgtg gtggctctcg
     6301 cttgttgggg tatgcccttt ccggtttcgg ctcagttcac gcgatgcata ttcatctcag
     6361 gcaccgccga tgcgctcgtc aagccgaacg cggtcgaggc atcgcaaaag tcgctacgcg
     6421 aggcgatcga caaatggaag gcggataacg gcgtgaccgc gccggtaagt gagtacgcgc
     6481 agaaaccccg gccggttccc tattggcgga gcgaggtcga tcccagcctg ttccttccgc
     6541 ccgatgtggt tagcgacacc gcctacactc tgtgctggaa gggcgtggtg tcaccggtcg
     6601 tttgtaccac cggcgccaag ctctgctggt gaggcgcgct tgaacttctc ggcgagatcg
     6661 ctgccgagca gctcgtcata gacggcaagc gttgcctctt gcatctgcgc cagcgtgaaa
     6721 ttcgccgcca catgctcccg cgcgcgaaca ccgatcgccg cccgctccgc agccttaagg
     6781 ccgagcgccc ggccgatgcg gcttgcgagc gaggcaatgt cgcgcggcgg aaacagccat
     6841 ccggtaaacc cggcctggcc ttgcccttga gcgacgatgg tctccggcag cgcgccgaga
     6901 tccggaacga tcacgggaca gcccatggct tgcgcttcgg cgctggtgcg cccgaaggtc
     6961 tcgggcaccg tcgatgtggt gaccgcgacc tgggatgcga gataggccgc cggcatctcc
     7021 cggcaatgtc cgacgagccg taccttgtcg ccgaggccat ggcgcgcgat cagcccgaga
     7081 agctcctcgc gataggcgtc gcgtccgggc gcgtcgccgg cgagaatgac cacagcgttg
     7141 cggagcgcgc cctcggcatt ggcgagcgcc gccgcggcga tcaggtcgcg ctgccccttg
     7201 atgctggtga gccgcgcggc gtggagcacg atcttggtgc cttgaggaac gccccaagca
     7261 gccctgagct tctcgacgga ctgcgcatcg acggcggcag gatcgaagac gtagcggtcg
     7321 acgccgcggt agataacgcg gatgcgatcc ttggcacgtg gcgcgcgcgc ttcgatcacc
     7381 gacgcggtgt agcgggaatt ggcgatgacg cggtcgccct tcgccatgac gctgttatag
     7441 gcggccttga acgggccgac ctcgccatag gcgccgtgat aggtggtgac gaacggtttg
     7501 ccggtgcggc gcgccgcgag caatgcgctc caagccggtg cccggctccg cgcatgcacg
     7561 agatcgacct tgcggtcctt tatcagcttg gtgaggcgcc tgatattggc aaggatggtg
     7621 aacggcgatt tgctcgccac cggaagcgtc acgatctcgc cgcccgcctt gcccacttca
     7681 tcggccatgc ggccgccttc ggtggcgacc agtgcgctcg cgcccgcgcg ggacagcgcc
     7741 tgggcgatct cgatggtcgc caattcgctt ccccctgcat cgaggcgggg cacgacctgg
     7801 agtatggtca ccatgaatga catcctaacc gctaacgttc aataaaatcg gccaatattc
     7861 acccccctgt actccttgta aacaaacaaa aattagctta tgttatccct ccgttagccg
     7921 ccgttagatc gggggtttgt tatccctacg ttatccaggc taacagaaaa agagggacag
     7981 ttggcacgcc gaacaatcac cgacctaggg gttgctgcac tcaaaccgaa ggccgctcgc
     8041 tacaatttc
//
LOCUS       DATNAE010000081        18685 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2797627, whole genome shotgun sequence.
ACCESSION   DATNAE010000081 DATNAE010000000
VERSION     DATNAE010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 18685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18685
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2797627"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..549)
                     /locus_tag="VN518_01685"
     CDS             complement(106..549)
                     /locus_tag="VN518_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03315.1"
                     /translation="MPGEDPTSSPDEIAVLQAYIDLINSEREAIWARHNALLVANSLI
                     IGALAISPAALWQNKWAALGMISAGAIISAAWLGITLEGWSAMKRHADLARKFASTYF
                     KHLPNALDETLCDHAQTNIHHLILLVIGVFVLMYVGLGYIRLVSA"
     gene            796..2415
                     /locus_tag="VN518_01690"
     CDS             796..2415
                     /locus_tag="VN518_01690"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXE03316.1"
                     /translation="MTSRSNAAPGRFGLLDVVVLIAMAVIATAFAAGLISNSGIDMMA
                     GAIAGAALFVVMASSHYVITRQARSATVAGRLDELEEALVVLDGDLQRIDQVEDDVAR
                     LDLLTDRVERLDQAISEYGGAAPATGVSGPVVELLTQDVEQLRGRLDVLRTDFAAQTR
                     AQRDEIGAELKSLEGLIKDLSRELMAASPAFAAASTEDLTETSEIPLRLEERLERVSL
                     AELGDQPAGDDDAVDELAADDTLAGETVVVTEQTVVFVTEPAPVADEVVVVVPEPAEP
                     VADIAPGDAMLRDLRDAIEGNRIDLYLQPIVTLPDRKLRFYDASTRVRTGDDAFMAPG
                     DYLELAEREHLMPRIDNVMLVKCVQLLRRLGPESRLKGVFCNLSAQSLLDHDFFPELV
                     EFMEENSTLADNLTFQLSQRAVLDIGADELAGLKTLGKLGFVFSLDHVADLDVDFAAL
                     RDHFFRYVKIEAKTLLHDMAEARASIPASDMTSYLDRFDLKLIVEKVDDEASLERLMD
                     YGVDLAEGDLFARPRPVTPEMFRELAEADAA"
     gene            complement(2456..2758)
                     /locus_tag="VN518_01695"
     CDS             complement(2456..2758)
                     /locus_tag="VN518_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03317.1"
                     /translation="MLYRFMTAGAAVCALSLLGFTGQADATTRLMLKASPALTILAAD
                     EENPEVENLLDPEADNGVPGGPTEATPGDEPKPEGEMKAKPEGGDSEMKEMKEEGR"
     gene            complement(2958..3167)
                     /locus_tag="VN518_01700"
     CDS             complement(2958..3167)
                     /locus_tag="VN518_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03318.1"
                     /translation="MLFAGSGEAQAGYYQPSYRYSAPNYGYQDYPAYGGYGYRGYGNG
                     HDEIRELQRLFPSTNWPPSLRYYSQ"
     gene            3403..4269
                     /locus_tag="VN518_01705"
     CDS             3403..4269
                     /locus_tag="VN518_01705"
                     /inference="COORDINATES: protein motif:HMM:TIGR01459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01459 family HAD-type hydrolase"
                     /protein_id="HXE03319.1"
                     /translation="MTISADTIPVLGSIGELGKSYSAWLVDIWGVMHNGVRAFPAAVE
                     ATRRFREQGGIVVLLSNSPRPSEGLQRQLRSLGVTDQSFDATVSSGDLTRHELAKHQG
                     ARIFHLGPPRDLPIVDGLDVTRVDAKDAELIVCTGLLDDETETPEDYEGLLHELAGRK
                     LTMLCANPDHLVERGHKLVYCAGALAQLYAEDGGEVIYAGKPYAPIYELAAEIIAERA
                     GRKLSRSEILAIGDGVHTDMAGATGFGLDAVFVASGLHVGPGGEGLDDVRLAELFEGR
                     KPPRAAMPALAW"
     gene            4354..4806
                     /locus_tag="VN518_01710"
     CDS             4354..4806
                     /locus_tag="VN518_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HXE03320.1"
                     /translation="MTTNGNGTTTKGYFFEDLAVGMEASYAKKITNDDVLAFADLSGD
                     TNPVHLDDAYAARTMFKQRIAHGFLTASLFSTVLGTKLPGPGCIYLSQNLKFRAPVHI
                     GDEVVATGKITSLDSEKGRAVLAMQAAVNGKSVLDGEAVMMVPRRERA"
     gene            4842..5828
                     /locus_tag="VN518_01715"
     CDS             4842..5828
                     /locus_tag="VN518_01715"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HXE03321.1"
                     /translation="MEVITGWRDVPAPAKGAVLAIGNFDGVHRGHQAVLGRARDLAKA
                     TGKRSGAVVFEPHPREFFAPDQPFFRLTPLPVKLELLEALGLDETFVIEFGPELAGLS
                     AETFASEVVAKGLGAAHVVVGYDFTYGKGRTGTPAQLKALGKKLGFDVDIVEPVASDG
                     VVFSSSKVRDYLRQGEMVEAAEQLGYWWRVRGKVERGAGLGKGLGFPTVNLQLGKGQD
                     IAHGIYAVRVAHKGRCYPAAGYVGPRPTFGGDKPVLEAYLLDFDGDLYGEEIEVEFIE
                     KLRHDATFADGESLAAQMHHDCAQVRGLLTMLAGGDPMARYPLGRALSGGTG"
     gene            5903..9259
                     /gene="ileS"
                     /locus_tag="VN518_01720"
     CDS             5903..9259
                     /gene="ileS"
                     /locus_tag="VN518_01720"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007563282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HXE03322.1"
                     /translation="MAKEKSKQPSSRAKPKRAAKPAGKAAADAKSVKPAAKAKTITAK
                     TAKRKTAKAKTAKRPASKPKSAKGSANPRSAKSTRTPPAKAKAAVKLAVRPKAKAKPK
                     ARPVSKAPVEKPLAPVARAIARPPQIGRDWSATLFLPKTDFPMKAGLPEREPELLKRW
                     ARLRLYDRMREEAMGREKFILHDGPPYANGNIHIGTGLNKILKDVINRSQRMMGKDAN
                     YVPGWDCHGLPIEWKIEEENYRSKGKTKPDLSDPAAMIAFRQECRAYAQHWLDVQREE
                     FKRLGVEGDWDHPYTTMSFDAEATIAAELMKFAMNGLLYRGSKPVMWSVVEKTALAEA
                     EVEYQEITSHTIFVKFPIIRATTGNLDPYELLGASIVVWTTTPWTIPGNRSISYSNSI
                     SYGLYEVTGAPDGNWAKKGEKFVIADALADNVKAAARIESWNRLNDVKGEMLRYSVAA
                     HPLRGHGYDFDVPLLAGEHVTADTGTGFVHTAPGHGREDFEVWTANERLLADRKIDSS
                     IPFTVDADGYFTKDAPGFEGKRVIDDKGNFGDANEAVIQELIKANALIARGRLRHDYP
                     HSWRSKKPVIFRNTPQWFIAMDSAIDVDAPTDTLRNRALAAISETRWVPPQGENRSRG
                     MIDTKPDWVVSRQRAWGVPITVFRNINPNSESFDDVVPSKDYPWSEALIERVREAFAK
                     EGADAWFKPGARERFLGGLVDDPSEWEKVDDILDVWFDSGSTHAFVLEVRPDLAWPAS
                     LYLEGSDQHRGWFQSSLLEACGTRGRAPYEAVLTHGFVVDEDGRKMSKSLGNVVAPQD
                     VIRQSGAEILRLWAMSSDYAEDLRIGQDIIKANVESYRKLRNTLRFMLGNLAHYEASL
                     AVAYAEMPELERHMLSRLAELDQIVRAGYEAFDFKRVFHALLNFCVNDLSAFYFDIRK
                     DALYCDPYDSVTRRSALTVLDSLCGALTAWLAPMLAFTMEEAWLNRFPGEASSVHLRQ
                     FATIPAPWRDEALAEKWRKVRAVRRVVTGALEIERKEGRIGSSLEAAPKVYVADRDLR
                     EALAGVDLAEISITSGAELLSGVGPEGAFRLDDVPGVAVVFARAQGTKCARSWKILED
                     VGADPEFPELSLRDAEAVRQFDARHPLAAE"
     gene            9266..9775
                     /gene="lspA"
                     /locus_tag="VN518_01725"
     CDS             9266..9775
                     /gene="lspA"
                     /locus_tag="VN518_01725"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HXE03323.1"
                     /translation="MRHLWGPLSPLGLGVAAVTLILDQAHKAWMLYVYDIGAKGTVRV
                     TPFLDLVLVWNQGVSYGMLQQRTELGRFLLILFAFGAAIALAVWLARINSRLTAICIG
                     LIIGGAIGNAIDRILYGAVADFFSFHAFGYEWYIFNIADTAIVAGVVGLLYESVFGGH
                     KKVSNPSKM"
     gene            9812..10429
                     /locus_tag="VN518_01730"
     CDS             9812..10429
                     /locus_tag="VN518_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03324.1"
                     /translation="MGHVATRILRLFGRGLLSLVACGLALALGGCGGIDFQGKVFDYA
                     GLSSLGKEQEDVRMTERAPLLVPPNTHKLPAPGSPDVTRADWPADTDKERKRLAAARQ
                     AEERKKAAEGEPNNPYAGKPNLLDQVLGRNKTPEEDPLDVPEPDPSDKTAEDKARERT
                     AATPQKPVDQSLNAPATPAADDPFHPAAPDSYKGMSSPSGNNANY"
     gene            10593..12017
                     /locus_tag="VN518_01735"
     CDS             10593..12017
                     /locus_tag="VN518_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HXE03325.1"
                     /translation="MFDQLAGALDARTGLILAAFSLASMAVAVAVDGARATPRVVEFT
                     LGNGLQVLVIPDHRAPVITQMIWYKVGAADEPPGSSGIAHFLEHLMFKGTDAIPPGQF
                     SKIVAKNGGEDNAFTNHDVTAYFQRVAKDRLPTVMAMEADRMANLRLSKEDVATERDV
                     ILEERRSRVDNDPSSILQEQVMAALYTKHPYGIPIIGWAHEVAALDRDDALSFYKRFY
                     APNNALLVVAGDVEPDEVRKLAEESFGKLPPHGGLNGRSRPPEPEHQAPLRVELEDAR
                     AGRMTVQRYYLAPSYASAAPGEAEALDLMMRIAAIGSVSRLYKRLVIEQKKAASAGGW
                     YSDSALDTGRLAFYAIGADKVSPAELETAMDVVIADLREHGVTQADLDRARSAFLAEF
                     VYANDSQSRMARHYGWRLATGMTVEEVESWPERLKKVTVDNIRDVARKYLVDKNSVTG
                     ILVPAPDKTRSGEQPAHAPSPGRS"
     gene            12023..13351
                     /locus_tag="VN518_01740"
     CDS             12023..13351
                     /locus_tag="VN518_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HXE03326.1"
                     /translation="MRQTRFAAIALLAALAASSADAMEIKRVTSPGGIEAWLVESHAN
                     PLIAMRFAFRGGAAQDPQDRQGLAYFVSGMMDEGAGGLDSVAFQERLQSLATRIDFDA
                     GRDVMLGNVQMLAANKDESFDLLRLAMSKPRFDADAIERVRGQIIAGLKFDENDPEEV
                     ASLAWDRLAFRDHPYGRPIKGTKASIAAITRDDLQGYLRRVFARDKLAITVVGDIDAE
                     TLGRALDHVFGALPQRSELAPVADVKPPLGPAREIIEMNVPQSVAQFGHRAFSRKDDD
                     FMAAYILNYIIGGGGFSSRLMEEVREKRGLAYSVHSNLFPYQHGAVFVGNVATQNERV
                     GQSLEVIESELRRLAEEGPSAEELAGAKSYLTGAYALRFESSSSIANQLLWIQIEDLG
                     IDYIDRRNTLIENVSLDDIRRVAKRLIEADRLITTIVGKPVEARPKAAPG"
     gene            13361..14668
                     /locus_tag="VN518_01745"
     CDS             13361..14668
                     /locus_tag="VN518_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009494682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HXE03327.1"
                     /translation="MALSQSVEGCLEAKIGEAGLAQAALDRTLSRLQPRLDSLREDYT
                     SGALPLLHVPERRDDIEAARAAVAALTKDARTLVFFGTGGSSLGGQTLAQLGGWGIPG
                     DDGNGGQRDRPRTRFYDNLDPRTLDRALSSLDLGTTRFVIISKSGNTPETLVQAIAAI
                     EAVRAAGLDARIPALFLGLTEPRSKATNGLRDLCEALSIPTLPHDPNIGGRFSALTNV
                     GLLPALARGLDVEALRDGAGSVVSALLGARTPLDFAPAVGAAVAIGLAKERGVKVSVM
                     LPYSDRLSRFAAWYVQLWGESLGKQGEGTTPVAALGPVDQHSQLQLYLDGARTTVMKC
                     CGSGPRVSADLAKIAHAEYLSGHAAGDLVAAQQKAIPEALLAAGRPVRTIDFERLDER
                     ALGALMMHFMLETILAGHLLGVDPFDQPAVESGKVITRRYLGA"
     gene            14688..16445
                     /gene="mutL"
                     /locus_tag="VN518_01750"
     CDS             14688..16445
                     /gene="mutL"
                     /locus_tag="VN518_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HXE03328.1"
                     /translation="MAIRQLPPNLINRIAAGEVVERPASVVKELIENAIDAGAARIDV
                     VAHGGGLNLIRVTDDGSGMDRDDLVLAVERHATSKLKDDDLSHILTLGFRGEALPSIA
                     AVARLTITSRARGADQAHEIAVDGGRKGELKPAAIGEGTRIEVRDLFYATPARLKFMK
                     SERAESAAIADVVKRLALANPEIAFSLSTGDRATLRLDPCPPGLLEHGLARLGRILGE
                     EFVTDALTVRAARGSLAVEGFAGLATLHRPNSLGQHLVVNGRPVRDKLLAGAVRGGYG
                     DLVPNGRHPMLALFLTLPPDEVDVNVHPAKTELRFRDAQAVRALIVTALHRALGIAQH
                     RATASLSEQALSRMTRPAGVSMPNRQAFGFADRAQAPLAPISTPSAVTHVNADDDSAV
                     AFPLGAARAQVHDTFIIAETENSLVIVDQHAAHERLVYERLKKAFANGGPSRQMLLIP
                     EVVELDAEAADRLAGAGAELERLGLVVEAFGQSAVIVREVPALLGQGNLQGLMRDLAD
                     ELAEGEGSLVVSERLDHVLSTMACHGSVRAGRRLNATEMNALLRDMEETPFSGQCNHG
                     RPTYVELKLADIEKLFQRR"
     gene            complement(16459..17472)
                     /locus_tag="VN518_01755"
     CDS             complement(16459..17472)
                     /locus_tag="VN518_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03329.1"
                     /translation="MKLFKVLGFASLLAAMIAFAAQASAFTSGGWEGQANHDEDGTFR
                     DCTMTADYANGITLAFIISRDFGWGLVLANDKWNLQVGSEEVVTLAVDKLAPIHGIAK
                     VVDAHGILVPLENADPVVEAMRHGHSLAVVTPAGEVSFKLSGTRDAIAALASCVSENL
                     AAEKAGSIEAKAPKDSEDSGNKLFTPSEAAAFASDLLASAGITNYEMENPQETPMPSF
                     DVVWKYANGIVAALVGYKDMGAADLDEAANEVMVDDAKNCKGTFASGKKISEPADQVS
                     VKRLYTSCRSTGKSVEIHYTLVETGSGHLILIAHLNLGEATGDVANADSAFLHGAVLR
                     SFK"
     gene            complement(17499..18110)
                     /gene="rsmD"
                     /locus_tag="VN518_01760"
     CDS             complement(17499..18110)
                     /gene="rsmD"
                     /locus_tag="VN518_01760"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HXE03330.1"
                     /translation="MRIVAGKFRAKRIEAPKGLTTRPTSDRVRQALFNVLEHGAPQFD
                     FAGARVLDLFAGSGALGLEAMSRGARFCLFVEESADARASIRRNVEALSLTGITKIWR
                     RDATKLGEAGTLAPFDLIFLDPPYSKGLGLRALQSAAAGGWIKDGAIAVLEDRADAEI
                     ELPAAFQQLDARIYGETKIVVIRVFPSLGLSLRHGPSPGDVLE"
     gene            complement(18187..18685)
                     /locus_tag="VN518_01765"
                     /pseudo
     CDS             complement(18187..18685)
                     /locus_tag="VN518_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="pseudouridine synthase"
BASE COUNT         3330 a         6190 c         5862 g         3303 t
ORIGIN      
        1 cagcgagaag aagcgcgcct gatctccctg accgaacgct gcgttctcaa agtaagcgct
       61 gagctaaccg aaggcagccg cgcattgccg cagggatcgt cgtgctcagg ccgagacgag
      121 gcggatatag cccagtccca cgtacataag cacgaagacc ccgatcacca gcaggatgag
      181 gtgatgaatg ttcgtctggg catggtcgca gagagtctcg tcgagcgcat tcgggagatg
      241 cttgaagtag gtcgaggcga attttctcgc aagatcggca tgccgcttca tcgccgacca
      301 tccctcaagc gtaatcccga gccacgcggc actgataata gcgccggcgc tgatcatgcc
      361 gagagccgcc catttgttct gccacagcgc cgcaggactg attgcgagcg cgccgatgat
      421 cagcgaattg gccacgagca gcgcgttatg gcgcgcccag atggcttccc gctccgagtt
      481 gatcaggtcg atataggcct ggaggaccgc gatctcgtcg ggcgaactgg ttgggtcttc
      541 accgggcatg gcgggcggag tgtgtcacgg cggcgaaaat cgcgccagtg cgagcgaggc
      601 tggcggccgg cttctgccgt catgcgcatc gagaagcgcg ccatctggtc tttccatcgc
      661 gccgggttta tcgtcttggc ggccagggtg actgctctta gacttgagca ttgccgatct
      721 tcatttatgc ttgtcgatgg cgtgctggcg cgggcactgc cctgagggac cggccagaag
      781 ccgaagtgaa gagccatgac ttctagatcg aacgctgctc ccgggcgttt tggcctgctc
      841 gatgtggtcg tcctcatcgc catggcagtg atcgcaaccg cctttgccgc cggtctcatc
      901 agcaattcag gcatcgacat gatggccggc gccatagccg gggcggcgct gtttgtcgtc
      961 atggcctcct cgcattacgt gatcacccgc caggcccgct cagccactgt cgccggccgc
     1021 ctcgacgagc tcgaagaagc ccttgtcgtc ctcgacggcg acttgcagcg catcgatcag
     1081 gtcgaggacg atgtggcgcg gctcgatctc ttgaccgacc gggtcgagcg gctcgaccag
     1141 gctatctccg aatatggcgg cgccgcgccg gcgaccggcg tgagcggccc ggttgtcgag
     1201 ctcctaacgc aagacgtgga gcaactgcgc ggccgccttg atgtgctgcg cacggatttc
     1261 gccgcacaga caagggcgca gcgtgacgag atcggcgccg agctcaaatc gctggaaggc
     1321 ttgatcaagg acctttcgcg cgagttgatg gcggcgtctc cggctttcgc tgcagcttcc
     1381 accgaggatc tcaccgagac ctccgagatc cccctacggc ttgaggagcg cttggagcgc
     1441 gtaagcctcg ccgagcttgg cgaccagccg gccggcgatg atgacgcagt cgatgagctt
     1501 gccgccgatg acacccttgc cggcgagacg gtggtggtta ccgagcaaac cgtcgttttc
     1561 gtcaccgagc ctgcgccggt tgccgatgaa gtcgtcgtcg tcgtgcccga gccggcagag
     1621 cccgtcgccg atatcgctcc cggcgacgcc atgctgcgag atttgcgcga tgccatcgag
     1681 gggaaccgga tcgacctcta tctccagccg atcgtgactt tgcccgaccg caagctccgc
     1741 ttttacgacg cctcgacccg cgttcgcacc ggcgacgatg cgttcatggc gccaggcgac
     1801 tatctggagc tcgccgaacg cgagcacctc atgccgcgca tcgacaatgt gatgctggtc
     1861 aagtgcgtgc agctgttgcg ccgtctcgga ccggagtcgc ggctcaaggg cgtgttctgc
     1921 aatctctcgg cccagtcgct gctcgaccac gatttcttcc ccgagctcgt cgagttcatg
     1981 gaggagaaca gcacccttgc cgacaacctc accttccaac tgagccagcg cgccgtgctc
     2041 gatattggcg ccgatgagct tgccggtctc aagaccctcg gcaagctcgg cttcgtgttc
     2101 tctctcgacc acgtcgccga tctcgacgtc gatttcgccg cgctccgcga tcacttcttc
     2161 cgctacgtca agatcgaggc aaaaaccttg ctgcacgaca tggcggaagc gcgggcaagc
     2221 atccctgctt ctgacatgac gagctatctc gaccgcttcg atctcaagct gatcgtggag
     2281 aaggtcgacg acgaggcaag cctcgagcgc ctgatggact atggcgtcga tctcgcggaa
     2341 ggcgatctct tcgcgcggcc aaggccggtg acgcccgaga tgtttcgcga gcttgccgag
     2401 gccgacgcgg cgtaggcgcc gtcacccttt gggcgacggc gcagctggtt gaaggctatc
     2461 tgccttcttc cttcatttct ttcatttcgg aatcgccgcc ttcaggcttt gccttcatct
     2521 ccccttcggg cttgggctcg tcgcctggag ttgcctcggt ggggccgcct gggacgccgt
     2581 tgtcggcctc ggggtcgagc agattctcga cttccgggtt ctcctcgtct gccgcgagga
     2641 tggtgagggc tggcgatgct ttgagcatca gccgcgtggt ggcatcggcc tggccggtga
     2701 aaccaagaag cgaaagcgcg caaactgcgg cgccggcggt cataaagcga tagagcatgt
     2761 tcatctccct agtgagcctt gcgggggccc ttaatgcgat ggcggggaaa tagcaccaaa
     2821 cacagaacgt aatgttacaa gaataggcaa agcttgtgca aagttgcggt taagcgcgcg
     2881 caaatgcttc aaaggcggcg cttacgccaa ccaggcaggc cggcgccctc tcgagcgccg
     2941 ggttccattc ctgaagccta ctgcgaataa taccgcaggc ttggcggcca gttggtcgag
     3001 gggaatagcc tctgcagctc gcggatctcg tcatggccgt tgccatagcc gcggtagcca
     3061 tagccgccat aggccggata atcctggtag ccatagttcg gggcagagta ccggtaggaa
     3121 ggctggtagt agccggcctg ggcctcgcct gaacctgcga acaacaacgc tgcgaatgcg
     3181 gccgaagcgg ccaaagccat taatttcgga agtctgggaa ggtgaggcat agttatctcc
     3241 tatcgccgtc ccctccctcg gcggggggta tctggggact tggcccgcag actgcaatct
     3301 gggcttcaca agaaagtgag cgtccaaggg gtgcgaccag gtcgccggcc tctctgacag
     3361 ccatcgcctt gaccctcccg gcccaagcct ctacgggagc ctatgaccat cagcgctgac
     3421 accatcccgg tgctcggctc catcggagag ctgggcaaga gctattccgc ctggctcgtc
     3481 gatatttggg gcgtgatgca taacggcgtg cgcgcctttc ccgccgccgt tgaagccacg
     3541 cgccgcttcc gcgagcaggg cggcatcgtc gtgctcctct ccaactcgcc gcggccgagc
     3601 gagggcctgc agcgtcaatt gcgcagcctc ggcgttaccg atcaaagctt tgacgccacg
     3661 gtctcctcag gcgacctgac ccgccacgag ctcgccaagc accaaggcgc ccgcatcttc
     3721 catctcggac ctccgcgcga cctgccgatc gtcgatggtc tcgatgtcac gcgcgtcgat
     3781 gccaaggacg ccgagctcat cgtctgcacc ggcctgctcg acgacgaaac cgagacgcct
     3841 gaggactatg aaggtctgtt gcacgagctt gccgggcgga agctcaccat gctctgcgcc
     3901 aatcccgacc atctggtcga gcgcggccac aaactcgtct actgcgccgg cgcgctcgcg
     3961 cagctctatg ccgaggatgg cggcgaggtg atctatgccg gcaagcccta cgcgccgatc
     4021 tacgagcttg ccgccgagat catcgccgaa cgcgccggac ggaaactgtc gcggagcgag
     4081 atcctggcca tcggcgacgg cgtacacacc gatatggcgg gcgccaccgg cttcggcctc
     4141 gacgccgtgt tcgtggcgag cgggctgcat gtcggacccg ggggcgaggg gctcgacgat
     4201 gttcgcctcg ccgagctgtt cgagggccgc aagcccccgc gcgcggcgat gcccgcgctc
     4261 gcctggtagg ccggcggcgg gaatccttct tattgcagtg cagcaaaccc ttggatatgt
     4321 tcgcctttgc gaaatcagaa gcaggggcga gtgatgacca cgaatggcaa cgggacgacg
     4381 accaaaggct atttctttga agatctcgcc gtcggcatgg aggcgagcta cgccaagaag
     4441 atcaccaacg acgacgtcct ggccttcgcc gatttatcgg gagacaccaa tccggtgcat
     4501 ctcgacgacg cctatgccgc gaggacgatg ttcaagcagc gcattgccca tggctttctg
     4561 accgcgagcc tgttctccac cgtgctcggc accaagctgc ccgggcccgg ctgcatttat
     4621 ctctcccaga acctcaaatt ccgtgcgccg gtgcatatcg gcgatgaggt ggtggcgacg
     4681 ggcaagatca cgagcctcga ttcggagaag ggaagggctg tcctcgccat gcaggccgct
     4741 gtcaacggca agagcgtgtt ggacggcgag gctgtgatga tggtacctcg ccgcgagcga
     4801 gcctagttgc gcccgcatat ggacatcata aggatggacg aatggaggtc atcaccggtt
     4861 ggcgggacgt ccctgcgcca gccaaaggcg cagtgctcgc catcggcaat ttcgatggcg
     4921 tccatcgcgg ccatcaggcg gtcctcggcc gcgcccgcga tcttgccaaa gccaccggca
     4981 agcgctcggg cgccgtggtg ttcgagccgc atcctcgtga gttcttcgcg cccgaccagc
     5041 ccttcttccg cctgaccccc ctgccggtga agctcgagct gctcgaggcg ctcgggctcg
     5101 acgagacatt cgtcatcgag ttcgggcccg agcttgccgg cttgagcgcc gagaccttcg
     5161 cctcggaggt cgtcgccaag gggctcggcg ccgcccatgt cgtggtcggc tatgacttta
     5221 cctacggcaa gggccgcacc ggcacgcccg cccaactcaa ggcgcttggc aaaaagctcg
     5281 ggttcgacgt cgacatcgtc gagccggtgg cgagcgacgg cgtggtgttt tcctcgagca
     5341 aggtgcgcga ctatctccgc cagggcgaga tggtcgaggc cgccgagcag ctcggctatt
     5401 ggtggcgggt gcggggcaag gtcgagcgtg gcgcgggcct cggcaagggg ctcggcttcc
     5461 ccaccgtcaa tctgcagctc ggcaaagggc aggatatcgc ccacggcatc tacgcggtca
     5521 gagtcgcgca taagggtcgc tgctatcctg ccgccggcta cgtcggaccg cgtccgacct
     5581 ttggcggcga taagccggtg ctcgaggcct atctcctgga tttcgacggc gacctctacg
     5641 gcgaggagat cgaggtcgag ttcatcgaga agctccgcca cgacgccacc ttcgccgacg
     5701 gagagtcgct cgccgcccag atgcaccacg actgcgcgca ggtccgcggc ctcctcacca
     5761 tgctcgcagg cggcgacccc atggcgcgct atcctctcgg gcgcgctctc tccggcggca
     5821 ctggataata agaggcggcg ttgctagaac gccgcggggc gtggcgcgcg gcatttgcag
     5881 gtaagggcgg aagcaaggac tcatggcgaa agagaaaagc aagcaacctt cctcgcgcgc
     5941 caagccgaag cgcgccgcca agcctgccgg caaggcggcg gcggatgcga aatcggtaaa
     6001 gcctgccgcc aaggccaaga ccatcactgc caagaccgcc aagcgcaaga ccgcaaaggc
     6061 caagactgcc aagcgcccag cctcaaagcc aaaatccgcc aagggctccg ccaacccgag
     6121 atcggcgaag tccacgcgca cgccgccggc caaagccaag gcggcagtga agctagctgt
     6181 ccggcctaag gcaaaagcca aaccgaaggc tcgtccggtc agcaaggcgc cggtcgaaaa
     6241 gcccttggca ccggtggcga gagcgatcgc tcggcccccg cagatcgggc gcgactggag
     6301 cgccaccctg ttcctgccca agaccgattt cccgatgaag gcagggctgc ccgagcgcga
     6361 gcctgagctg ctcaagcgct gggcgcggct tcgcctctac gaccggatgc gcgaagaggc
     6421 catgggacgg gagaagttca tcctgcatga cggcccgcct tacgccaacg gcaacatcca
     6481 tatcggcacc gggctgaaca agatcctcaa agacgtgatc aaccgctcgc agaggatgat
     6541 gggcaaggac gccaattacg ttcccggctg ggactgtcac ggcctcccga tcgagtggaa
     6601 gatcgaagag gagaactatc gctccaaggg caagaccaag ccggacctct cagacccagc
     6661 agccatgatc gcgtttcggc aggagtgccg cgcctacgca caacattggc tcgacgtgca
     6721 gcgcgaggag ttcaagcggc tcggggtcga gggcgactgg gatcatccct acaccaccat
     6781 gagcttcgat gcagaagcga ccatcgccgc cgagctgatg aagttcgcca tgaacgggct
     6841 gctctatcgc ggctcgaagc cggtgatgtg gtcggtggta gagaagaccg cgctcgccga
     6901 ggccgaggtc gagtatcagg agatcacgtc acacacgatc ttcgtgaagt tcccgatcat
     6961 ccgcgcgacg acggggaact tggacccgta cgaacttctt ggggcttcaa tcgtcgtctg
     7021 gacgacgacg ccttggacca tcccaggcaa ccgctcgatc agctactcaa acagcatctc
     7081 atacgggctt tatgaggtca cgggcgcgcc tgacggaaat tgggccaaga aaggcgagaa
     7141 gttcgtcatc gccgacgcgc tggcagacaa cgtcaaagcc gcggcgcgaa tcgaaagctg
     7201 gaaccggctg aacgacgtca aaggcgagat gctgcgatac tcagtcgccg cacatccact
     7261 acgcggtcac ggctatgatt ttgatgtgcc actgcttgct ggtgaacatg tcactgccga
     7321 caccggtacg ggattcgttc acacggctcc tggtcacggc agagaagact ttgaggtctg
     7381 gactgcgaat gagcgattac tcgctgatcg caagatagac tcgtcgatcc ccttcaccgt
     7441 cgatgccgat ggctacttca ccaaggatgc gcctggcttc gaaggcaagc gcgtcatcga
     7501 cgacaagggc aatttcggcg acgccaacga ggcggtcatt caggagctga tcaaggccaa
     7561 cgcgctcatc gcccgcgggc gcctccgcca cgactatccc cattcctggc gctcgaagaa
     7621 gccggtgatc ttccgcaaca cgccgcaatg gttcatcgcc atggacagcg caatcgatgt
     7681 ggatgctcct acagacactt tgcgcaaccg cgcccttgcc gccatcagcg agacgcgctg
     7741 ggtgccgccg caaggcgaga accgcagccg cggcatgatc gataccaagc cagattgggt
     7801 ggtgtcgcgc cagcgcgcct ggggcgtgcc catcaccgtg ttccgcaata tcaatccgaa
     7861 ctcggagagc tttgacgatg tcgtgccgag caaggactat ccctggtcgg aagcgctcat
     7921 cgagagagtg cgcgaagcct ttgccaagga aggcgctgac gcttggttca aaccgggcgc
     7981 gcgagaacgc ttcctcggcg gcctggtcga cgacccatca gagtgggaaa aggtcgacga
     8041 catcctcgac gtttggttcg actccggctc cacccacgcc ttcgtgctcg aggttcgccc
     8101 cgacctcgcc tggccggcct cgctctatct cgaaggctcg gaccagcatc gcggctggtt
     8161 ccaatcctcg cttctcgaag cctgcggcac gcgcggccgc gcgccttacg aggccgtgct
     8221 cacccatggc ttcgtcgtcg atgaggacgg ccgcaaaatg tcgaagtcgc tcggcaatgt
     8281 ggtggccccg caggacgtga ttcgccagtc aggcgccgag atcctcagac tgtgggcgat
     8341 gagctcggac tacgccgagg acctgcgcat cggccaggac atcatcaagg cgaacgttga
     8401 gtcctatcgc aagctcagga acacgcttcg gttcatgctc ggcaatcttg cccattacga
     8461 ggcaagcctt gccgtcgctt acgccgagat gccggagctc gagcgccaca tgctctcccg
     8521 cctcgccgag ctcgaccaga tcgtgcgtgc cggttacgag gccttcgact tcaagcgcgt
     8581 gttccacgct ctgctcaatt tctgcgtcaa cgatctctcg gccttctatt tcgacatccg
     8641 caaggacgcg ctctattgcg atccatacga cagcgtgacg cggcgctcgg cactcaccgt
     8701 gctagatagt ctgtgtggcg cgctcaccgc ctggcttgcc cccatgctcg ccttcaccat
     8761 ggaggaggct tggttgaacc gcttccccgg cgaggcaagc tcggtgcatc ttcgccagtt
     8821 cgctaccatc ccggccccct ggcgcgacga ggcgcttgcc gagaaatggc gaaaggtgag
     8881 ggcggtccgc cgcgtggtca ccggcgcgct cgagatcgag cggaaagagg gccgcatcgg
     8941 ttcgagcctc gaagccgcgc ccaaagtcta cgtcgccgac cgggacttgc gcgaagcgct
     9001 tgccggcgtc gatctcgccg aaatttccat cactagcggc gccgagctct tgagtggcgt
     9061 gggtcctgaa ggggcctttc gcctcgacga cgttcccggc gtcgccgtgg tctttgcgcg
     9121 cgcccagggc acgaaatgcg cccgctcgtg gaagattctt gaggacgtcg gcgccgatcc
     9181 cgaatttccc gagctgtcgc tgcgcgacgc cgaggcggtc cgtcagttcg acgcccgcca
     9241 cccgctggcg gcggaatagg cccgcatgcg ccacctgtgg ggaccgctat cgccactcgg
     9301 gctcggggtc gccgcggtca cgctgatcct agaccaggcc cacaaggcct ggatgctcta
     9361 cgtctatgac atcggcgcca aggggaccgt gcgcgtcacc ccgtttctcg atctcgttct
     9421 cgtctggaac cagggcgtga gctacgggat gcttcagcaa cggaccgagc tcggccgctt
     9481 cctcctgatc ctgttcgcct ttggcgccgc aatcgccctt gccgtgtggc tcgcgcgcat
     9541 aaattcgagg ctcaccgcca tctgcatcgg cctcatcatc ggcggggcca tcggcaacgc
     9601 catcgatcgc atcctctatg gcgcagttgc cgatttcttc tccttccacg ccttcggcta
     9661 tgagtggtac atcttcaata tcgccgatac cgccatcgtt gcgggcgtcg tcggacttct
     9721 gtatgaatcg gtatttgggg gacacaaaaa ggtctcaaac ccttctaaga tgtgaagaaa
     9781 gcccggtctt acgggcattc cagggggaat cgtggggcac gtcgcaaccc gcatcttgag
     9841 gctgtttggc aggggcctct tgtcgctcgt tgcctgtggc ctagcgcttg ctcttggcgg
     9901 ctgcggcggc atcgatttcc agggcaaagt cttcgactac gccggccttt ccagcctcgg
     9961 caaagagcag gaggacgtga ggatgacgga gcgcgcgccg ctcctcgttc caccaaacac
    10021 ccataagctt cccgcgccgg gcagccccga cgtcacgcgc gccgactggc cggctgatac
    10081 cgacaaggag cgcaagcggc tcgccgcggc aaggcaggct gaagagcgca agaaggcggc
    10141 cgagggcgag ccgaacaatc cttatgccgg caagccgaat ctgctcgatc aggtgcttgg
    10201 caggaacaag acgcctgagg aagacccgct cgacgttccc gagcccgatc cgtcggacaa
    10261 gacggccgag gataaggccc gcgaacgcac cgccgccacg ccgcaaaagc cggttgatca
    10321 gtcgctgaac gcgccggcca cgccggcagc cgacgatccc ttccacccgg ccgcgcccga
    10381 cagctacaag ggcatgtcca gcccctccgg caacaacgcc aattattgac gccaagcgcg
    10441 gcgacgcccg cgcggcccta gcgaattggc ttgaaaaatc cgggggcagg gctgaaatgt
    10501 ggggcaatcc cgcccgtcaa gccgttaccc ataaggtttg agggctgacc ctttggctcc
    10561 ttcacacccg gtcacgatcc atggtgatat cgatgttcga tcagcttgcg ggcgcgctcg
    10621 atgcacggac gggcctgatc ctggccgcct tcagcctcgc ctccatggcc gttgcggtcg
    10681 cggtggacgg cgcgcgcgcc acgccccgcg tcgtcgaatt cacccttggg aacggcctcc
    10741 aggtcctggt catccccgac catcgcgcgc cggtgatcac ccagatgatc tggtataagg
    10801 tcggcgccgc cgacgagccg ccggggtcct ccggcatcgc ccatttcctc gagcacctga
    10861 tgttcaaggg caccgacgcg atcccgcctg gccaattctc caagatcgtg gccaagaacg
    10921 gcggcgagga caacgccttc accaaccatg acgtcaccgc ctatttccag cgcgtggcca
    10981 aggatcggct gccgacggtg atggccatgg aggcagaccg catggccaat cttcgcttga
    11041 gcaaagagga cgtggcgacc gagcgcgacg tgatcctcga ggagcgccgc agccgcgtcg
    11101 acaacgaccc gtcgagcatt ctgcaagagc aggtgatggc ggcgctctac accaagcacc
    11161 cttacggcat ccccatcatc ggttgggcgc atgaggtggc ggcgctcgat cgcgacgatg
    11221 cactctcctt ctacaagcgc ttctacgcac cgaacaacgc tctgctcgtc gtcgccggcg
    11281 atgtcgagcc cgatgaggtg cgaaagctcg ccgaggagag cttcggcaag ctgccgccgc
    11341 atggcgggtt gaatgggcgc tcgcgcccgc cagagcctga gcatcaagcg ccgcttcgcg
    11401 tcgaactcga agacgcccgc gccggccgca tgaccgtgca gcgctattat ctcgcgccga
    11461 gctatgcctc ggccgcgcca ggcgaagccg aggcgctcga cctcatgatg cgcatcgccg
    11521 ccatcggctc cgtgagccgg ctctacaagc gcctggtcat cgagcagaag aaggcggcaa
    11581 gcgccggcgg ctggtattcc gattctgccc tcgacaccgg caggctcgcc ttctacgcca
    11641 tcggcgccga caaggtctcg ccggccgagc tcgagacggc catggacgtc gtgatcgccg
    11701 atctgcggga gcatggcgtc acccaagccg acctcgaccg cgcccgcagc gcctttcttg
    11761 ccgagttcgt ctatgccaac gacagccaat cgcgcatggc gcgtcactat ggttggcggc
    11821 tcgccaccgg catgaccgtc gaggaggtcg aaagctggcc cgagcggctt aagaaggtga
    11881 cggtcgacaa catccgcgac gtggcgcgga aatatctcgt cgacaagaac tcggtgacgg
    11941 ggattctggt gccggcgcct gacaagacga gaagcggtga gcaaccggcc catgccccaa
    12001 gccccggcag gtcttaggcg ccatgcggca gacgcggttt gccgccatcg cgctgctggc
    12061 ggcgctcgcc gcaagttctg ctgacgccat ggagatcaag cgcgtcacca gtcccggcgg
    12121 catcgaggcc tggctcgtcg agagccacgc gaatcccctg atcgccatgc gcttcgcctt
    12181 ccgcggcggc gccgcgcaag acccgcaaga caggcagggc ctcgcctatt tcgtatcagg
    12241 catgatggac gaaggcgccg gcggcctcga ttcggtcgcc tttcaggaac gcttgcagtc
    12301 gcttgccacg cgcatagatt tcgatgcggg ccgcgacgtg atgctcggca acgtgcagat
    12361 gctcgccgcc aacaaggacg agtcgttcga cctgttgcgt cttgccatgt cgaagccgcg
    12421 cttcgatgcg gatgcgatcg agcgggtgcg cggtcaaatc atcgccggat tgaagtttga
    12481 cgagaacgac cccgaggagg tcgcttcgct tgcctgggat cgtctcgcct tccgcgatca
    12541 cccttatggg cgcccgatca agggcaccaa ggcgtccatc gccgccatca cccgcgacga
    12601 tttgcaaggc tatttgcggc gcgtgtttgc ccgcgacaag ctcgcgatca ccgtggtcgg
    12661 cgacatcgat gccgagacgc tcggccgcgc actcgatcat gtgttcggcg cgctgccgca
    12721 acgctcggag ctggcgccgg tcgccgacgt caaacctccg ctcgggcctg cgcgtgagat
    12781 catcgagatg aacgtgccac aatcggtggc gcaattcggt catcgcgcct tctcgcgcaa
    12841 ggacgacgac ttcatggccg cctacatcct caattacatc atcggcggcg gcgggttctc
    12901 ctcgcgcctg atggaagagg tgcgcgagaa gcgcgggctt gcctattcgg tgcactcgaa
    12961 cctttttccc tatcagcacg gcgccgtgtt cgtcggcaac gtggcaacgc agaatgagag
    13021 ggtcggccaa tccttggagg tgatcgagag cgagctcagg cgtctcgccg aggaggggcc
    13081 gagcgccgag gagctcgccg gcgccaagag ctacctcacc ggcgcctatg cgcttcgctt
    13141 tgagtcctcg tcgagcatcg ccaaccagct tctgtggatt cagatcgaag atctcggcat
    13201 cgactatatc gaccggcgca acacgctgat cgagaacgtg agcctcgacg acatcaggcg
    13261 cgtggcgaaa cgcctcatcg aggccgatcg cctgatcacc accatcgtcg gcaagccggt
    13321 cgaggcaagg ccgaaggcgg cacctggcta gggctcgctt atggctttat cgcaatcggt
    13381 cgaaggctgc cttgaggcga agatcggcga ggctggcctc gcgcaggctg cgctcgaccg
    13441 cactctctct cgcctgcaac ctcgcctcga ctccttacgc gaggactata cgagcggagc
    13501 gcttcccttg ctgcacgtgc ccgagcggcg cgacgacatc gaggcggccc gcgccgccgt
    13561 tgctgcgctg acgaaagacg caagaaccct cgtgttcttc ggcaccggag gttcaagctt
    13621 gggcggccag acgctcgccc aactcggcgg ttggggcatt cccggcgacg acggcaatgg
    13681 cgggcaacgc gaccgcccgc gcacccgctt ctacgacaat ctcgatcccc gcacgctcga
    13741 tcgcgcgctt tcgagcctcg acctcggcac cacccgtttc gtgatcatct ccaagtcggg
    13801 caacacgccg gagactctgg tgcaggccat cgccgccatc gaggcggtgc gtgccgctgg
    13861 tctcgatgcc cgcatccccg cgctgttcct tggcctcact gagccgcgca gcaaagccac
    13921 caacggcctg cgcgacctgt gcgaagcctt gtcgatcccc acgcttccgc acgaccccaa
    13981 catcggcggc cggttttcgg cgctgaccaa tgtcggcctc ttgccggcgc ttgcccgcgg
    14041 cctcgacgtg gaggcgctgc gcgacggcgc agggtccgtc gtaagcgcgc tgctcggggc
    14101 gcgcacgccg ttggacttcg cgcctgcggt cggcgccgcc gtcgccatcg gcctcgccaa
    14161 ggaacgcggc gtcaaggtga gcgtgatgct gccctatagc gatcggcttt cgcgcttcgc
    14221 cgcctggtac gtgcagctct ggggcgagag cctcggcaag caaggcgagg ggacgacgcc
    14281 ggtcgcggca cttggtccgg tcgatcagca cagccagctt cagctctatc tcgacggcgc
    14341 gcgcaccacg gtgatgaagt gctgcggcag cgggccgcgc gtctcggccg atctcgccaa
    14401 gatcgcccac gccgagtacc tctcggggca cgccgccggc gatctcgtgg ccgcgcagca
    14461 aaaggcgatc cccgaggcct tgctcgcggc cggccggcct gtgcgcacca tcgacttcga
    14521 acggctcgac gagcgggcgc ttggcgccct gatgatgcat ttcatgctgg aaacgatcct
    14581 cgccggccat ctcttgggcg tcgacccgtt cgaccagccg gcggtagaat cgggtaaagt
    14641 catcacgcgc cgctatttgg gcgcgtgaaa cgcctcgagg gccttggatg gccatacgcc
    14701 agctgccgcc gaacctcatc aaccgcatcg ccgcgggcga ggtggtcgag cgcccggcga
    14761 gcgtcgtcaa ggagctgatc gagaacgcca tcgacgccgg tgctgcccgc atcgatgttg
    14821 tcgcccatgg cggcggcttg aacctgatcc gcgtcaccga cgatggaagc ggcatggacc
    14881 gcgacgatct cgtcctggcg gtagagcgcc acgccacctc caagctcaag gacgacgatc
    14941 tctcccatat cctcacgctt ggcttccgcg gcgaggcgtt gccgtccatc gctgccgtcg
    15001 ccaggctcac catcacgagc cgcgcccgcg gcgccgatca ggctcacgag atcgcggtcg
    15061 atggcgggcg taaaggcgag ctcaagcctg cggcgatcgg cgagggtacg cgcatcgaag
    15121 tgcgcgacct gttctatgcg accccggcgc ggctcaaatt catgaaatcc gagcgcgccg
    15181 agagcgcggc catcgccgat gtggtgaagc gcttggcgct cgccaaccct gagattgctt
    15241 tcagcctctc gaccggagac cgcgccacgt tgcggctcga tccttgtccg cctgggctgc
    15301 tcgagcatgg gctcgcgcgg cttggccgca tcctcggcga ggagttcgtg accgacgcgc
    15361 ttacagtgcg cgccgcacgc ggatcgcttg ccgttgaagg cttcgccggc ctcgccaccc
    15421 tgcatcgccc caacagcctc gggcagcatc tcgtggtcaa tggcaggccg gtgcgcgaca
    15481 agctgctcgc cggtgccgta cgcggcggtt acggcgacct cgtgcccaac gggcggcacc
    15541 ccatgcttgc ccttttcctc acgttgcctc ccgacgaggt cgacgtgaac gtgcatccgg
    15601 ccaagaccga gcttagattc cgcgatgcgc aagcggtgcg cgccctgatc gtcaccgcgc
    15661 tgcatcgggc gcttggcatc gcgcaacatc gcgccacggc gagcctgtcc gagcaggcgc
    15721 tgtctcgcat gacgcggccg gcgggcgttt ccatgcccaa ccgtcaagct ttcggcttcg
    15781 ccgaccgggc ccaggcgccg ctcgcgccaa tctccacgcc gagcgccgtc acccatgtga
    15841 acgccgacga cgacagcgcc gtcgcttttc ccctcggcgc cgcgcgggcg caagtgcacg
    15901 acaccttcat catcgccgag acggaaaatt ccctcgtcat cgtcgatcag cacgcggcac
    15961 atgagcggct ggtctatgag cgcctgaaga aggccttcgc caatggcggc ccctcccgcc
    16021 aaatgctgct gatcccagaa gtggtcgagc tcgatgccga ggctgccgac cgtcttgccg
    16081 gagccggagc agagctcgag cggcttgggc tggtggtcga ggccttcggt caatcggcgg
    16141 tcatcgtgcg cgaggtcccg gctctgctcg gccagggcaa cctccagggc ctgatgcgcg
    16201 acctggccga cgagcttgcc gagggcgagg gcagtctcgt cgtctccgag cggctcgacc
    16261 acgttctgtc gaccatggcc tgccacggca gcgtgcgggc agggcgccgc ctcaatgcca
    16321 ccgagatgaa cgcgcttctt cgcgacatgg aggagacgcc gttctcgggc cagtgcaatc
    16381 acggccggcc gacttatgtc gagctcaagc tcgccgatat agagaaactg ttccagcgcc
    16441 gctgaataaa gccgccgcct atttgaagct gcgcagcacc gcgccatgca ggaaggcgct
    16501 gtcggcattg gccacgtcgc cggtggcctc gccgagattg agatgggcga tcaggatcag
    16561 atggccgctc cccgtctcca ccaaagtgta gtggatctcg accgacttgc cggtagagcg
    16621 gcaggacgtg tacagccgct tcacgctcac ctgatctgca ggctcgctga tcttcttgcc
    16681 tgaagcgaag gtgcctttgc aattcttggc atcgtcaacc atgacctcgt tcgccgcctc
    16741 gtcgagatcg gcagcgccca tatccttgta gccgaccagc gccgcgacga tgccgttcgc
    16801 gtatttccac accacgtcga aactcggcat cggggtttct tgcgggttct ccatctcgta
    16861 attggtgatg ccggccgagg cgagcaggtc ggaggcgaac gccgccgcct cgctcggggt
    16921 gaagagtttg ttgccgctgt cttcgctgtc cttcggggcc ttggcctcga tgcttcctgc
    16981 cttctcggcc gccagattct cgctgacgca actggccagg gccgcgatcg cgtcgcgcgt
    17041 gccggaaagc ttgaagctca cttcgccggc cggcgtgacg acggcgagcg agtggccgtg
    17101 gcgcatggct tccaccaccg gatcggcgtt ttcgagcggc accaaaatgc cgtgcgcgtc
    17161 gaccaccttg gcgatgccgt gaattggggc gagcttgtcg accgcgagcg tcacgacctc
    17221 ctcgcttccc acctgcaaat tccatttgtc gttggcgagc accagccccc agccgaaatc
    17281 gcggctgatg atgaaggcga gcgtgatgcc gttggcatag tccgcggtca tggtgcaatc
    17341 gcggaaggtg ccgtcttcgt cgtgattggc ttgtccttcc cagccgcctg aggtgaaggc
    17401 gctggcctgc gctgcgaaag cgatcatcgc ggcgaggaga gaggcaaagc ccaggacttt
    17461 gaatagcttc atggcaccct cagtccccgg aacaactgtc attcaagcac atctccgggg
    17521 ctcgggccat ggcgcaggct caacccgagt gacggaaaaa ctcggatcac gacaatttta
    17581 gtctctccgt agatacgtgc gtcgagctgt tgaaacgccg ccggaagttc gatttccgcg
    17641 tcggctcgat cctccaaaac cgcgatagcg ccgtccttga tccatcctcc ggcagcggcg
    17701 gattgaagcg cccttagccc aaggcccttg ctgtaaggcg gatcgaggaa gatgaggtcg
    17761 aagggtgcaa gcgtgccggc ctcgccgagc ttggtcgcgt cacgccgcca gattttggtg
    17821 atgccagtga gcgacagcgc ctcgacattg cggcggatgg aagcgcgcgc gtccgcactc
    17881 tcctccacga acaggcagaa gcgcgcgccg cgcgacatcg cctcgagccc cagagcgccg
    17941 gagcctgcga acagatcgag cacgcgggcg ccggcgaaat cgaattgcgg cgcgccgtgc
    18001 tcgagcacgt tgaacagcgc ctggcgcacg cggtcagatg tcggccgcgt ggtgagcccc
    18061 ttcggcgcct cgatgcgctt ggctctgaac ttgccggcta cgatacgcat aagagagggt
    18121 tgatattcat cgccgccgcg ccgctcgcag cctttggttc tcgctgctca agccaagctc
    18181 gccggcgagc ttggcgccca actgatccat gaccacgcgc tgcttgatct cgctcacttc
    18241 gccaaccggc aaatccccga gcgcgaacgg tccgaaggaa atgcggatca ggcggttgac
    18301 ggtgaggccg agcgcttcgg cgagccgctt cacctcgcgg ttcttgcctt cgcgcaagcc
    18361 caaggtcagc cagacattgc tcccttgctc gcgatcgatc cttgcttcca ccggcccata
    18421 ctgcacgccg tcgatggtca tgcccttggc gagcttgtcg aggtcggctt ggccgaccct
    18481 gccgtgcgcc cgcacgcgat agcggcgcaa ccagccggtg ctcggcagct ccaagtgcct
    18541 tgcaagctct ccgtcggtgg tgagcagcag aagcccttcg gtgttgagat cgagccgccc
    18601 caccgagacc acgcgaggaa gctcgggcgg caatgcctcg aacacggtct tccgcccttg
    18661 cgggtccttg tggctggtca cgagc
//
LOCUS       DATNAE010000082         2867 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2496861, whole genome shotgun sequence.
ACCESSION   DATNAE010000082 DATNAE010000000
VERSION     DATNAE010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2496861"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="VN518_01770"
     CDS             complement(<1..478)
                     /locus_tag="VN518_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HXE03331.1"
                     /translation="MRTYLDFEKPIAELESKVAELKALAAEQHSMSIEEELAKLEAKA
                     REALEETYSELTPWQKTQVARHPERPHFRHYIDGLVQDFTPLAGDRFYAEDQAIIGGI
                     GRLKGRSAMIIGHEKGSDTEGRIKHNFGMARPEGYRKAQRLMEMADRFSLPVVSLVD"
     gene            complement(611..1585)
                     /gene="xerD"
                     /locus_tag="VN518_01775"
     CDS             complement(611..1585)
                     /gene="xerD"
                     /locus_tag="VN518_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HXE03332.1"
                     /translation="MGTKGRSGSSNESELGLFLDMLMAERGAAPKTIEAYTGDLGEFL
                     AFLATKRKTARDASADTVRAYLAQLSRKGLAPTSRARKLSAMRQFFRFLLAEGIRADD
                     PCSAIDSPKLGRPLPKLLSLAEVESLLETAAQEVEQAGEHVAKRRALRLYALLETLYA
                     TGLRVSELIGLPRGVLVSDDQVMTIKGKGGRERLVPLNESARKALADHLAAVAADEAQ
                     GRARSPWLFPSGGGGQHLTRQRFGQELKALAIAAGIEPSRVSPHVLRHAFASHLLERG
                     ADLRTVQQLLGHADISTTQIYTHVIEERLRRLVEQHHPLAQVHLTKRS"
     gene            complement(1590..>2867)
                     /locus_tag="VN518_01780"
     CDS             complement(1590..>2867)
                     /locus_tag="VN518_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03333.1"
                     /translation="EVKIAALERAGRVNELIEKLGEANEPDALAAYALALLAAGRNDE
                     ACAVQLGTGQPDGAAKRASVLIPAYCAAASGDNEAARNALDVARNNGVDVGFASRAIA
                     GQGGRGPLTKSVDVLDYLFLKLGQSANRADIAAKAGPELLFLLARDDEASPELRVAAA
                     ERAASLNIIDGTTLATVYRDEAPRLPKSAQSPAALRAKLFASLEAQTSEKIRAESIDA
                     LLASGKDARIEIPMAQAMAQSSQGRDTHASSFAETGVRVAALAGDDQAAWDLTEQAGD
                     RVRSWQLLLATTDPLSERARSALDSGVDIALKAGLPGPLLQRLVTALDALGEEVPIPL
                     WDLAAKTPQPDDGYLPPTGLLSTLKEAADAGDTGRTILLAAAVFGPDGPQGAHLIALG
                     DGLRALKRVGLDAEARRLAFEALYAHWPSRGKA"
BASE COUNT          466 a          942 c          964 g          495 t
ORIGIN      
        1 tgtcgaccag gctcaccacg ggaagcgaga agcggtcggc catctccatg agccgctggg
       61 ccttgcggta gccttccggc cgcgccatgc cgaaattgtg cttgatccgg ccttcggtgt
      121 cggagccctt ctcatgcccg atgatcatgg ccgagcgtcc cttgaggcgt cctatcccgc
      181 cgatgatcgc ctggtcctca gcgtagaagc ggtcgccggc gagcggggtg aaatcctgaa
      241 cgagcccgtc gatgtagtgg cggaagtgcg gccgctccgg gtgccgggcg acttgcgtct
      301 tctgccaggg ggtcagctcc gaataggtct cctccagagc ctcgcgcgcc ttggcttcga
      361 gcttggcaag ctcctcctcg atggacatgg agtgctgctc ggcggcgagc gctttgagct
      421 cggcgacctt gctttcaagc tcggcaatgg gcttttcgaa atcgagataa gtgcgcattt
      481 ggctaaaggt ggcccatggg acccgtcctc gatgggctgc cgcgctggaa tggcggcggg
      541 tacgtgcttt taagtggcct tggggcgaaa ccgtgtcaac cggcaaccct gcctccgact
      601 ttgggcgaga ctagctccgc ttggttaaat gtacctgagc cagggggtga tgttgctcga
      661 caaggcgccg cagccgctcc tcgatgacgt gggtgtagat ctgcgtggtc gagatatcgg
      721 catggcccaa gagctgctgc accgtgcgca aatcagctcc ccgctccagc aaatgactgg
      781 caaaggcatg acggagaaca tgcggcgaga cgcgcgacgg ctcgatgccc gcggcgatgg
      841 caagcgcctt caactcctgg ccgaagcgct gccgggtcag atgctgcccg cctccgcctg
      901 agggaaacag ccaaggcgac cgcgcgcggc cttgtgcctc gtcggcggcg accgccgcaa
      961 gatgatcggc gagcgccttg cgcgccgact cgttgagcgg cacgaggcgc tcgcgtccgc
     1021 ccttgccctt gatggtcatc acctggtcgt cgctgacaag caccccgcgg ggcagcccaa
     1081 tcagctcaga gacgcgcaag cccgtggcgt aaagcgtctc gagcaacgcg tagaggcgga
     1141 gcgcccgccg cttggccacg tgttcgcctg cctgttccac ctcttgcgcc gccgtctcga
     1201 gcaggctctc gacctcggca agggagagga gcttcggcaa cgggcgtccg agcttcgggc
     1261 tgtcgatcgc cgagcaggga tcgtcggcgc ggatgccttc cgccaagagg aagcggaaga
     1321 actggcgcat ggctgaaagc ttgcgcgccc gcgaggtggg ggcgagcccc ttgcggctga
     1381 gctgggcaag ataggcgcgc acagtgtcgg ccgaagcgtc gcgcgcggtc ttccgcttgg
     1441 tggcaaggaa ggcaagaaac tcgccgaggt cgcctgtgta ggcctcgatg gtcttcgggg
     1501 cggcgccgcg ctcggccatg agcatgtcga ggaacagccc aagctccgac tcgttgctgc
     1561 ttccggatcg gcctttcgtg cccaatgtct caggcctttc cgcgcgaagg ccaatgggcg
     1621 tagagcgcct cgaaggcaag gcgccgcgct tccgcatcga gcccgacgcg cttcaacgcc
     1681 cgcaagccat cgccgagcgc aatcaaatgg gcgccttgcg gaccgtcagg cccaaacacc
     1741 gcggcggcaa gcagaatggt gcggccggtg tcgccggcgt ccgccgcctc cttcaacgtg
     1801 ctgagcagac ccgtcggcgg caggtagccg tcgtcgggct gaggcgtctt ggcggcaagg
     1861 tcccagagcg ggatcggcac ctcctcgcca agcgcatcga gcgcggtgac cagccgttgc
     1921 agcaagggac ctggcagccc ggccttcaat gcgatgtcaa cgcctgaatc gagcgcgctc
     1981 cgcgcccgct cgctcaaggg atccgtcgtt gccagcagca attgccagct gcgcacccgg
     2041 tcgccggcct gctcggtcaa atcccaggcg gcttggtcgt cgccggcaag cgccgccacg
     2101 cgcacgcccg tctcggcgaa acttgatgcg tgcgtatcgc gtccttggct cgattgcgcc
     2161 atggcttgcg ccatcggaat ttcgatcctc gcgtccttgc cgctcgcgag caaagcgtcg
     2221 atcgactcgg cgcggatctt ctccgaagtc tgtgcttcaa gagaggcaaa cagcttcgcc
     2281 cgcaaagccg caggcgattg cgccgatttg ggaaggcgag gtgcctcgtc gcggtagacg
     2341 gtcgcaagcg tcgtgccatc gatgatgttg agcgaggctg cgcgctccgc cgcggcgacg
     2401 cgaagctctg gcgaagcttc atcgtcgcgc gccaaaagaa agagaagctc cggaccggcc
     2461 ttggcggcga tgtcagcccg attggccgac tggccgagct tcaagaaaag atagtcgagc
     2521 acgtcgacgc tttttgtcag aggcccgcgc ccgccctgcc cggcgatggc gcgcgatgca
     2581 aacccgacgt ccacgccatt gttgcgggcg acatcgagcg cgttacgggc agcttcgttg
     2641 tcgccgctcg cggcggcgca ataggccggg atcaggacgc ttgcgcgctt ggcggcgcca
     2701 tcgggctgcc cagtgccgag ttgcaccgcg caggcctcat cgttgcggcc tgcggcgagc
     2761 agcgccaagg cgtaggccgc gagcgcgtct ggctcgttgg cctcgccaag cttctcgatc
     2821 agctcgttga cgcgcccggc gcgctcaagc gccgcgatct tcacctc
//
LOCUS       DATNAE010000083         4477 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2499999, whole genome shotgun sequence.
ACCESSION   DATNAE010000083 DATNAE010000000
VERSION     DATNAE010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4477
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2499999"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VN518_01785"
     CDS             <1..351
                     /locus_tag="VN518_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03334.1"
                     /translation="AGMTIGTLFTLFVTPAVYTYLARDHQKAKAEAEAALAHATAATH
                     GDAVPETVVEADTAAEAGMLFPAPEEPVPAEDQLSPEATAFSSAASAVSSQGHEQQTG
                     PRRGRGPKLPTAAE"
     gene            434..2452
                     /gene="accC"
                     /locus_tag="VN518_01790"
     CDS             434..2452
                     /gene="accC"
                     /locus_tag="VN518_01790"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HXE03335.1"
                     /translation="MFSKLLIANRGEIACRVIRTARRLGIKTVAVYSEADRDAMHVYM
                     ADEAVPIGPAQASQSYLDIGKIVAAAKATGAEAVHPGYGFLSENAAFARALQEAGIAF
                     VGPNVKAIEAMGDKIESKKLAAKAKVSTVPGHLGVIQDAKEAVKIAEEIGYPVMIKAS
                     AGGGGKGMRIAYSRKEVEEGFVSSKSEAKSSFGDDRVFIEKYVEEPRHIEIQVLGDKH
                     GNVIHLNERECSIQRRNQKVVEEAPSPFLDAKTREAMGAQAVALAKAVGYDSAGTVEF
                     IVDKNRNFYFLEMNTRLQVEHPVTELVTGLDLVEEMLKVAAGEKLSLKQKDVKLKGSA
                     VEVRIYAEDPQRNFLPSTGRLVRYRPPKLGTEGGVTTRLDSGVEEGGEISVFYDPMIA
                     KLVTHAPKRLKAIEEMGSALDAFAIDGFRHNIPFLAVLMRNERWREGRLSTKFIAEEF
                     PEGFKPPKPVGEVRDTLVAVAAAIDHMSNVRRRTITQQMHGEPVRFAETRVVELGQER
                     VKVSVAGGLDEPITVTLGADGKAKPKAITLASSWWFGAPVWEGTIDGAPAAVQVRPIL
                     NGVLLTSQGVEAQARVFTERETELMAQMPEKVKPDMSKFLLCPMPGLVVSLAVSEGQE
                     VAAGDALCVVEAMKMENVLKAERDAKVSKILAKPGDSLNVDAVILEFA"
     gene            2474..2782
                     /locus_tag="VN518_01795"
     CDS             2474..2782
                     /locus_tag="VN518_01795"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007563796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HXE03336.1"
                     /translation="MTESGNKQRTVTVKIEGRVQGVYYRAWTNETARSLNLDGTVRNM
                     SDGSVEAVFSGPAQVVEDMLRRCEDGPKNAQVSKVTIMYEGGTVAPGFKVIGTDWQRR
                     "
     gene            complement(2783..3427)
                     /gene="lipB"
                     /locus_tag="VN518_01800"
     CDS             complement(2783..3427)
                     /gene="lipB"
                     /locus_tag="VN518_01800"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HXE03337.1"
                     /translation="MALPASKPQSTPQVEWLASTAPVPYPDALAVMDARAAAIARGEA
                     EDCVWLLEHPSIYTAGTSADPAELLGPDRFPVFKSGRGGRFTYHGPGQRVAYVMLDLT
                     RRGRDVRAFVRSLEDWLIATLARLGLEGERRPGQIGIFVQGAKLASVGVRVRNWVTLH
                     GVSFNVDPNLEHFAGIVPCGLADTPVTSLAALGVEAEMDKVDQALRETFAEFFS"
     gene            3635..3720
                     /locus_tag="VN518_01805"
     tRNA            3635..3720
                     /locus_tag="VN518_01805"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3669..3671,aa:Leu,seq:gag)
     gene            3798..>4477
                     /locus_tag="VN518_01810"
     CDS             3798..>4477
                     /locus_tag="VN518_01810"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HXE03338.1"
                     /translation="MQDAAGEVELRDEAGAIRSDFLHAVSAAIDEGDAAKVRELTLPL
                     HEADLADLIQLLRPEPRGVLITMLGDDLKAAALPELDEGVRNQVLEDMPPEQVAKALQ
                     QLDSDEAVYLLEDLDKDEQSDILAQLPHFERIALERSLEYPEDSAGRIMQTDLIAVPP
                     FWSVEQTIDFMRLADDLPDRFYEIFVVDPAYHLLGSVALNRLLRSKRSVTIEAITDRD
                     IHPIAVEA"
BASE COUNT          818 a         1429 c         1525 g          705 t
ORIGIN      
        1 gccggcatga ccatcggcac gctgttcacc ctgttcgtga cgccggccgt ctatacctat
       61 ctcgcgagag accaccagaa ggccaaggcc gaggccgagg cggctctcgc ccatgccacc
      121 gccgcgacgc acggcgacgc cgttccggaa acggtggtgg aggcggatac ggccgcggag
      181 gccggcatgc tcttcccggc gcccgaggag ccggtgccgg ccgaggacca attatcgccg
      241 gaggcgaccg ctttcagctc ggccgcttcc gccgtttcga gccaaggcca tgagcagcag
      301 acgggccccc gcaggggccg agggccaaag ctgcccacgg cggccgagta agcccttctt
      361 gcaccgcacc tagtttactc gccttgaggg agcctctaga aagcccttgg ccagtcaagg
      421 ggcggggacc cgcatgttct cgaagttgct catcgccaac cggggcgaga tcgcctgccg
      481 cgtcatcagg accgcgcgcc ggctcggcat caagaccgtg gcggtctatt ccgaggcgga
      541 tcgcgacgcg atgcacgtct atatggccga cgaggccgtt ccgatcggcc cggcgcaggc
      601 ctcgcagtcc tatctcgata tcggcaagat cgtcgccgcc gccaaggcga ccggcgccga
      661 ggcggtgcat cccggctacg gcttcctttc cgagaacgcc gccttcgccc gtgcgctcca
      721 agaggccggc atcgcctttg tcggacccaa cgtcaaagcc atcgaggcga tgggcgacaa
      781 gatcgagtcg aagaagctcg cggcgaaggc gaaggtctcg acggttcccg gtcatctcgg
      841 cgtgatccag gacgccaagg aggcggtgaa gatcgccgag gagatcggct acccggtgat
      901 gatcaaggca agcgccggcg gcggcggcaa gggcatgcgc atcgcctata gccgcaagga
      961 agtggaggaa ggcttcgtct cgtcgaagtc ggaggccaag tcgagcttcg gcgacgaccg
     1021 cgtgttcatc gagaaatatg tcgaggagcc gcggcacatc gagatccagg tgctcggcga
     1081 caagcacggc aacgtcattc atctcaatga gcgcgaatgt tcgatccagc gccgcaatca
     1141 gaaggtggtc gaggaggcgc cgagcccgtt cctcgacgcc aagacgcgcg aggcgatggg
     1201 agcgcaggcc gtcgctctgg ccaaggccgt cggttacgac tccgccggca cggtcgagtt
     1261 catcgtcgac aagaaccgaa atttctattt cctcgagatg aacacgcggc tccaggtgga
     1321 gcatccggtg accgagctcg tcaccgggct cgatctcgtc gaggagatgc tgaaggttgc
     1381 ggccggcgag aagctctccc tcaagcagaa ggatgtgaag ctcaagggct cggccgtcga
     1441 ggtccgcatc tatgccgagg acccgcagcg gaacttcctg ccctcgaccg ggcggctggt
     1501 gcgctaccgc ccgcccaagc tcggcaccga gggcggcgtc accacgcggc tcgactccgg
     1561 cgtggaggag ggcggcgaga tcagcgtctt ttacgacccg atgatcgcca agctggtgac
     1621 gcatgcgccg aaacggctca aggccatcga ggagatgggc agcgcgctcg acgccttcgc
     1681 catcgacggc ttccgccaca acattccctt ccttgcggtg ctgatgcgca acgagcggtg
     1741 gagggaggga cggctctcca cgaaattcat cgccgaggag tttccggaag gcttcaagcc
     1801 gcccaagccg gtcggcgagg tgcgcgatac gctggtcgcg gtcgccgcgg cgatcgacca
     1861 tatgagcaat gtgcgacgcc gcaccatcac ccagcagatg catggcgagc cggtgcgctt
     1921 cgccgagacg cgggtggtcg agcttggcca ggagcgggtg aaggtgagcg tcgcgggcgg
     1981 cctcgacgag ccgattaccg tgacgctcgg cgccgacggc aaagccaagc cgaaggcgat
     2041 cacgctggcc tcgtcgtggt ggttcggcgc accggtatgg gaaggcacga tcgatggcgc
     2101 gcccgctgcg gtgcaagtgc gcccgatcct caatggcgtg ctgctgacct cgcagggcgt
     2161 cgaggcacaa gcgcgggtgt tcaccgagcg cgagaccgag ctcatggcgc agatgccgga
     2221 gaaggtgaag cccgacatgt cgaaattcct gctttgcccg atgccggggc tggtcgtgtc
     2281 gctggccgtg agcgaaggcc aggaggtcgc ggcaggcgac gcgctctgcg tcgtcgaggc
     2341 gatgaagatg gagaacgtgc tgaaggccga gcgcgacgcg aaagtctcga agatcctcgc
     2401 caagcccggc gacagcctga atgtcgacgc ggtgatcctc gaattcgcgt gaggccgcgg
     2461 aaggcttcgc ataatgacag agtccggtaa caagcagcgc accgtcacgg tcaagatcga
     2521 ggggagggtg cagggtgtct attaccgcgc ctggaccaac gagacggcgc gctcgctgaa
     2581 cctcgacggc acggtgcgca atatgagcga cggcagcgtc gaggcggtat tctcgggtcc
     2641 cgcgcaggtg gtcgaagaca tgctgcggcg ctgcgaggac ggccccaaga acgcgcaggt
     2701 cagcaaggtg accatcatgt atgaaggcgg cacggtggcg ccgggcttca aggtgatcgg
     2761 caccgattgg cagcgccgct gactaactga agaactcagc aaaggtctcg cgcaacgcct
     2821 gatcgacttt gtccatctcg gcttcgacgc cgagcgcggc gaggctggtg actggcgtat
     2881 cggcgagacc gcacggcacg atgccggcga agtgctccag gttcggatcg acgttgaagc
     2941 tcacgccatg cagcgtgacc caattcctca cccgtacgcc gacgctggcg agcttggcgc
     3001 cttgcacgaa gatgccgatc tggccgggcc ggcgctcgcc ctccaggccg agacgcgcca
     3061 gcgtggcgat cagccagtct tcgagcgagc ggacgaacgc cctgacgtcg cggccgcgcc
     3121 gcgtaaggtc gagcatcaca taggccacgc gctggcccgg gccgtgatag gtgaagcgcc
     3181 cgccgcgccc ggacttgaag acgggaaagc ggtcgggccc gagcagctcc gccggatcgg
     3241 cgctggtgcc cgccgtgtag atggaggggt gctcgagcag ccagacgcag tcctcagcct
     3301 cgccgcgggc gattgccgca gcgcgtgcat ccatgacggc gagcgcgtcg ggatagggca
     3361 caggcgccgt gctcgccagc cattcgactt gaggcgtgga ttgcggtttg cttgcgggaa
     3421 gggccatggc cgtgagcgct tcgacgtcca acttggctgc gatcgtctag gctaaacctt
     3481 gcaccctcca atagggtttg ctaaggcagc gagcccagcc ggcgcttgcc gatcgggcat
     3541 tctatagcca tatcggggac ggaaagcctc gagattcctc gttttgaggc atggcccgcc
     3601 ggaacctctg tcgagtctcc ggaggtcaag cttagcggtc gtggcggaat tggtagacgc
     3661 gcagcgttga gggcgctgtg ggcttagccc gtggaggttc gattcctctc gaccgcacca
     3721 ctttccttcc ttcagtgcgt cacgcccggg ccgcggtccg gacaggggaa cgatagaacc
     3781 cgacgatcgg acaggccatg caggacgccg cgggcgaggt cgaactgcgc gacgaagcgg
     3841 gcgcgatccg ctccgatttc ctgcatgccg tcagcgcggc gatcgatgag ggcgacgccg
     3901 ccaaagtccg cgagctgacg ctgccgctgc atgaggccga cctcgccgac ctgatccaac
     3961 tgctgcgtcc ggagccgcgc ggcgtcctca tcaccatgct tggcgacgac ctcaaggccg
     4021 cggcgctgcc cgagctcgac gagggggtgc gcaatcaggt gctggaggac atgccgcccg
     4081 agcaggtggc gaaggcgctg cagcagctcg attccgacga agccgtctat ttgctcgagg
     4141 acctcgacaa ggacgagcag tccgacatcc tggcccaact tccgcatttc gagcgcatcg
     4201 cgctggagcg cagcctcgaa tatcccgaag actcggccgg ccgcatcatg cagaccgatc
     4261 tgatcgcggt gccgcccttc tggtcggtgg agcagacgat cgacttcatg cgcctggccg
     4321 acgacctccc cgatcgcttc tacgaaatct tcgtggtcga tcccgcctat cacctgctcg
     4381 gctcggtggc gttgaaccgg ctgcttcgct ccaagcgttc ggtgacgatc gaggccatca
     4441 ccgaccgcga tatccatccg attgccgtgg aggccga
//
LOCUS       DATNAE010000084         2294 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153 k141_88545,
            whole genome shotgun sequence.
ACCESSION   DATNAE010000084 DATNAE010000000
VERSION     DATNAE010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88545"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..532
                     /locus_tag="VN518_01815"
     CDS             173..532
                     /locus_tag="VN518_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006018801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HXE03339.1"
                     /translation="MPYVDGFVLPVPKKKVEAYKRMAKKAGKVWREHGAIEFKECIAD
                     DVPMGKLTSFPRSVKRKPNETVIFSYIVYKSRAHRDRVMAKVMQDKRIGDMMNIKDLP
                     FDAKRMIHGGFKVLVEA"
     gene            complement(560..1342)
                     /locus_tag="VN518_01820"
     CDS             complement(560..1342)
                     /locus_tag="VN518_01820"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXE03340.1"
                     /translation="MNWARKRAVDVHDETAGYFSAEYASDNVHDSPFRYGRSLIDELW
                     WKVVEELPAGAHCLDIGSGIGAYMARLLEAGLQLRGIEPSGEMRKLAQERVPEGLVSD
                     GSVTDLPAGDRTLDFIYAIEVFRYLDTADNMRGHREIFRALKPGGVYFGTYVNRWALD
                     FYRPFVAMQKARDTLLKATPRYHVEFETPASLRKRLAGVGFENIEIRGAMFAPLRPLH
                     KLSPKGATAVSRWTLPREAWLSDRALSRPFAGHLVAIARRGS"
     gene            complement(1360..>2294)
                     /locus_tag="VN518_01825"
     CDS             complement(1360..>2294)
                     /locus_tag="VN518_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03341.1"
                     /translation="WGVRDHATRETYGRNVLGGRQAVAAMLDLFHKRGIRATWATVGA
                     LLCESKDELIARIKHTAVSRAVLGSLEDIGPDERRDPFHFGASLARRIASCEGQEIGT
                     HTFSHRCALEPGETLKGFAADVSYALLQLREWGIRCKSIVFPRNQYAPAHLEACSLLG
                     LTHFRGNEQAWFYAAASGDRQTTPRRLCRLADSYVNLSGANVSVPREGEGPLINVPSS
                     RFLRPYHPRLAPLDGLRLRRIEKAMEHAALTGGTFHLWWHPENFGANLAENLQMLGRV
                     LDVFERLRGEHGMWSRSMHELAKRTAVAQAPRAA"
BASE COUNT          457 a          750 c          705 g          382 t
ORIGIN      
        1 gcatttttgc acatgaagaa attcgacatc gccgcgctga cgcgcgcctt cgacggaggt
       61 caatcgtgag cgacagcaaa gtcaattgcc agaaagcccg ctgatgcagc ggctgatcct
      121 cgtcaatcat tgaaacccga gccaggatcg gcgacccaca aggaggactg atatgcccta
      181 tgtcgatgga ttcgtgctgc cggtaccgaa gaaaaaggtc gaggcttaca agcgcatggc
      241 caagaaagca ggcaaggtct ggcgcgagca cggcgccatc gagttcaagg aatgcatcgc
      301 cgacgatgtg ccgatgggca agctcacctc gtttccgcgc agcgtcaaac gcaagccgaa
      361 cgagaccgtg atcttctcct atatcgtcta caagtcccgc gcccatcgcg atcgcgtcat
      421 ggcgaaggtg atgcaggaca aacggatcgg cgacatgatg aatatcaagg acctcccctt
      481 cgatgcgaaa cggatgatcc atggcggctt caaggtgctg gtcgaggcct gagccgcgcc
      541 tggagcgact cgctctaaat cagcttcccc gccgggcgat ggcgacgaga tgcccggcga
      601 agggccgcga caaggcgcgg tcggagagcc acgcctcgcg cggcaaggtc catctcgaga
      661 ctgcggtggc gcccttcggc gaaagcttgt gcagcggccg caagggagca aacatcgctc
      721 ccctgatctc gatgttctcg aagccgaccc ccgccagcct cttgcgcagc gaagcgggag
      781 tctcgaactc gacgtggtag cggggggtcg ccttcagcag cgtatccctc gccttttgca
      841 tcgccacgaa gggccggtag aagtcgagcg cccagcgatt cacataggtg ccgaaataga
      901 cgccgccggg cttcagcgcc cggaagatct cgcgatggcc ccgcatattg tcggccgtgt
      961 cgagatagcg gaacacttcg atcgcataga taaagtcgag cgtccgatcg ccggcgggca
     1021 agtcggtgac cgagccgtcc gacaccagtc cctcgggcac ccgctcttgc gccagcttgc
     1081 gcatttcgcc gctcggctcg ataccgcgca actgcagccc tgcttcaagc agccgcgcca
     1141 tataggcgcc gatgccggag ccgatgtcga ggcaatgcgc cccggccggc agttcctcga
     1201 caaccttcca ccacagctcg tcgatcagcg accggccgta acggaaggga gaatcgtgga
     1261 cgttgtcgct cgcatactcg gccgagaaat aaccggccgt ctcgtcatga acgtcgacgg
     1321 cgcgcttgcg ggcccaattc attcacgcac ctaaccgggt taggcggcgc ggggcgcctg
     1381 ggcaacggcc gtgcgcttgg caagctcgtg catggaccga ctccacatcc catgctcccc
     1441 acgcagcctc tcgaacacat caagcacgcg gccaagcatc tgcaaattct ccgcgaggtt
     1501 cgccccgaaa ttctccggat gccaccagag atgaaacgtg ccgccggtca aggccgcgtg
     1561 ctccatcgcc ttctcaatcc gcctgagccg caagccgtcg agcggagcca gccgcggatg
     1621 gtaagggcgc agaaaccggc tcgacggtac attgatcagc ggtccctccc cctctcgcgg
     1681 aaccgacacg ttcgctcccg atagattgac atagctgtcg gcgaggcggc aaagccggcg
     1741 cggcgtggtt tgcctgtcac ctgaagccgc ggcgtagaac cacgcctgct cgttgccgcg
     1801 gaaatgcgtg aggccgagca ggctgcacgc ttcgagatgt gctggagcgt attggttgcg
     1861 cggaaacacg atcgacttgc agcgtattcc ccattccctg agctgcaaca gagcgtagga
     1921 cacatcggcg gcgaaaccct tgagcgtctc gcccggctcg agcgcgcagc gatgcgagaa
     1981 ggtgtgcgtg ccgatctctt gcccctcgca ggaggcgatg cggcgcgcga gcgacgcgcc
     2041 gaaatggaag ggatcccgcc gctcatcggg tccgatatcc tcgaggctgc cgagcacggc
     2101 gcggcttacg gctgtatgct tgatccgggc gatgagctcg tccttgctct cgcagagcag
     2161 cgcgccgacg gtggcccagg tggccctgat gccccgcttg tggaaaagat cgagcatagc
     2221 cgctaccgcc tggcgacccc ccagcacgtt ccgcccatag gtctcgcgcg tggcgtgatc
     2281 cctcacgccc caca
//
LOCUS       DATNAE010000085         2576 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_392684, whole genome shotgun sequence.
ACCESSION   DATNAE010000085 DATNAE010000000
VERSION     DATNAE010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392684"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="VN518_01830"
     CDS             complement(<1..495)
                     /locus_tag="VN518_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HXE03342.1"
                     /translation="MVFFDDIRLVRDLLGDYDANLAVLEDRLGIQAVVNGNVVSLRGP
                     EQSRETAKSVLEQLYGRLAQGEMIGVGEVVGAIRHARTPEPVAEADQHDAKLPAAQQI
                     RTRKRLITARTPAQSAYLSALEAHDLVFATGPAGTGKTYLAVAYAALCLESGKCDRLI
                     LSRPA"
     gene            complement(600..1982)
                     /gene="miaB"
                     /locus_tag="VN518_01835"
     CDS             complement(600..1982)
                     /gene="miaB"
                     /locus_tag="VN518_01835"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012168539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HXE03343.1"
                     /translation="MAKTPQKLHIKTFGCQMNAYDSERMAEALADHGYASTDDPAEAD
                     LVILNTCHIREKAAEKVYSELGRVRALKRARNASGLDTMIAVAGCVAQAEGREMLARA
                     GVVDLVVGPQSYHELPSLIGRVREGGERVVATDFPAEDKFLALPERPLARAQPSAFIT
                     VQEGCDKFCTFCVVPYTRGAEYSRPVAAIIAEAERLARGGAREITLLGQNVNAYCGEG
                     PDGRDWSLAKLLKRLGDIPEIERLRYTTSHPRDMDDDLVALHGDEPKLMPYLHLPFQA
                     GSDRILAAMNRKHTADDYAELVGRIRTARPDIALSTDIIVGFPGETDADFEDTLALVR
                     RIGFARAFSFKYSARPGTPAATLPNQVPDKIARERLHVLQRLLDEEAKAFDAATVGRK
                     LKVLFEREGRKPGQITGRSPYLQAVYGEGPRDLIGQIVDVDIVSAGPNSLTGVIKSEF
                     EWREPVQAAV"
     gene            2167..>2576
                     /locus_tag="VN518_01840"
     CDS             2167..>2576
                     /locus_tag="VN518_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HXE03344.1"
                     /translation="MISQKAKYALRALVALAKEGDSLMIGEIAARKNIPRKFLEQILL
                     EMKRHGIVHSRRGKLGGYSLLMPPEKITYGQVLRIIDGPIAPLPCLSRIAYRRCADCK
                     TEETCEIRRVFARVAESAREVLDRTTIADSLDSPL"
BASE COUNT          437 a          828 c          831 g          480 t
ORIGIN      
        1 ggcggggcgt gacaggatca gccggtcgca tttgccgctt tccaggcaca gagcagcgta
       61 agcgacggcg agataggtct tgccggttcc cgccgggccg gtggcgaaca cgaggtcatg
      121 ggcctcgagc gccgagagat aggcgctctg cgccggcgtc ctggccgtga tcaggcgttt
      181 gcgcgtgcgg atctgctggg ccgcggggag cttagcgtcg tgctggtcgg cttccgccac
      241 gggctctggc gttcgcgcat gccggatggc gccgaccacc tcgccgaccc cgatcatctc
      301 gccctgcgcg agccggccgt agagctgctc gagcaccgac tttgcggtct cgcgcgactg
      361 ctcgggcccg cgcaaggaga ccacattgcc gttgaccacg gcctggatgc cgagccggtc
      421 ttcgagcact gcgaggttgg cgtcatagtc tccgaggaga tcgcgcacga gcctgatgtc
      481 gtcgaagaac accaccaggg gttcttcccg gccattggca gacgaggcgc ttgcgcgctt
      541 atcagacgcg gtcttgcgcc cacgcgtcaa tggcggtctc cgtccgctgg ccttgccggt
      601 cacacggccg cctgaactgg ttcgcgccat tcaaactccg atttgatgac accggtgaga
      661 ctgttcggcc ccgcgctcac aatgtcaaca tccacgatct ggccgatcaa atcccttggg
      721 ccctccccat acacggcctg gagatagggt gaacgcccgg tgatctgccc tggtttccgc
      781 ccctcccgct cgaacagcac cttgagcttg cgcccgacgg tcgccgcatc gaaggccttc
      841 gcttcctcat cgagcaggcg ttgcaggaca tgcaagcgct cgcgcgcgat cttgtccggg
      901 acctggtttg gcagggtggc tgcaggcgtg ccggggcgcg cgctgtattt aaaagagaag
      961 gcgcgtgcaa agccgatgcg gcggacaagc gccaaggtgt cttcgaagtc ggcgtcggtc
     1021 tcgcccggga agccgacaat aatatctgtg gagagagcga tgtcaggacg cgccgtgcgg
     1081 atgcgtccga ccagctcggc atagtcgtca gccgtatgct tgcggttcat ggcggcgagg
     1141 attcggtctg agcccgcctg aaacggcaga tgcagatagg gcatgagctt cggctcatcg
     1201 ccgtgcagcg cgacgagatc gtcatccatg tcgcgcggat ggctcgtggt gtagcggagc
     1261 ctctcgatct cgggaatgtc tccaagccgc ttgagcagct tggcgagcga ccagtcgcgg
     1321 ccatcggggc cttcgccgca ataggcattc acgttctggc cgagcagcgt gatctcgcgc
     1381 gcgcctccgc gggcgagcct ttcggcctcg gcgatgatgg cggcaacggg ccgcgaatat
     1441 tcggcgccgc gggtataggg cacgacgcag aaggtgcaga acttgtcgca gccttcctgc
     1501 acggtgatga aggccgaagg ctgggcgcgt gcgagcggcc gttcggggag ggcgaggaac
     1561 ttgtcctcgg ccgggaagtc ggtggcgacc acgcgctcgc ccccctcgcg cacgcggccg
     1621 atgagcgaag gaagctcgtg atagctttgc gggccaacca cgagatcgac cacgcctgcg
     1681 cgcgcgagca tctcccgccc ttcggcttgc gccacgcagc cggcgaccgc gatcatggtg
     1741 tcgaggccgc ttgcattccg cgcccgcttc agcgcccgca ccctgccaag ctcggaatag
     1801 accttctcgg cagccttctc gcggatatgg caggtgttga gaatgacgag atcggcctcc
     1861 gccggatcgt cggtcgaagc gtagccatga tccgccagcg cctccgccat gcgctcggag
     1921 tcgtaggcgt tcatctggca gccgaaggtc ttgatgtgca gcttctgggg cgttttcgcc
     1981 atcgcctgtt ggtgcggcac cggggcgcct ctcgtcaagg gtcgtggatc accggtcgaa
     2041 tttcgccaac gggtgaattg ccgctctatc cataaagtcg cactcgcctc gattgtttct
     2101 caagcgtcga ggcgctatat actcgactca ctcaatagga tttcatttga gaatcgtatt
     2161 tcgtccgtga tctcacaaaa agcaaaatat gccctccgcg ccctggtggc tctcgccaag
     2221 gaaggcgact cgctcatgat cggcgagatt gccgcccgca agaacatccc gcggaagttt
     2281 ctcgagcaaa tcctgctcga aatgaagcgc cacggcatcg tgcacagccg gcgtggcaag
     2341 ctcggcggct atagcctgct catgcctccg gagaagatca cctacgggca ggtgcttcgc
     2401 atcatcgacg ggccgatcgc ccccttgccg tgcctgagcc gcatcgccta ccggcgctgc
     2461 gccgactgca aaaccgagga aacctgcgag attcgccggg tttttgccag ggtggcagag
     2521 tcggcaagag aggtgctcga ccgcaccacc atcgccgatt ccctcgattc tccact
//
LOCUS       DATNAE010000086         2849 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3403787, whole genome shotgun sequence.
ACCESSION   DATNAE010000086 DATNAE010000000
VERSION     DATNAE010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3403787"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1969
                     /locus_tag="VN518_01845"
     CDS             <1..1969
                     /locus_tag="VN518_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXE03345.1"
                     /translation="KKRGADALEIYCINLNQKARAGRIDPLIGRGPEIQRTIQVLCRR
                     QKNNPLFVGDPGVGKTAIAEGLARRIVMGDVPEVLADSTIFQLDMGALLAGTRYRGDF
                     EERLKAVMREIEAYPGAIMFIDEIHTVIGAGATSGGAMDASNLLKPALQSGTLRCLGS
                     TTYKEYRQHFEKDRALVRRFQKIDVNEPSVPDAIEILKGLKPYFEDFHRVRYTSQAIK
                     SAVELSAKYIHDRKLPDKAIDVIDETGASQMLVPERQRRKTIGVKEVESTIATMARIP
                     PKTVSKSDAEVLRAIDQDLKRVVFGQDKAIEALAAAIKLSRAGLREPGKPIGCYMFSG
                     PTGVGKTEVARQLALLLGVELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDGI
                     DQHPHSVLLLDEIEKAHPDLFNILLQVMDHGKLTDHNGKRVDFQNVILIMTTNAGASD
                     LAKAAIGFNRAKREGDDEEAINRLFSPEFRNRLDAVITFANLPPEVIAKVVEKFIFQL
                     EAQLGDRNITIELTPEANAWLAERGYDDKFGARPLARVIQEHIKKPLAEEILFGALKD
                     GGIVRVLVEEKAGEKALGFEYLKADAPVRPRDPEDIDPEGEGPDGSGPKGPPKAGGPK
                     PRVRRGGSRKPRAARRSVGASLVPKVPLGTK"
     gene            complement(1976..2398)
                     /locus_tag="VN518_01850"
     CDS             complement(1976..2398)
                     /locus_tag="VN518_01850"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HXE03346.1"
                     /translation="MAYDPDNIFAKILRGEIPCHKIYEDEDTLAFLDIMPRAEGHALV
                     ITKEKARDLFDIKPEALARLMAVVQKLTPRIQEAMGAEGVLIQQFNGAAAGQTVFHLH
                     VHIVPRRQGEALKPHAGKMEDQAELAATAEKIRKKLAS"
     gene            complement(2534..>2849)
                     /locus_tag="VN518_01855"
     CDS             complement(2534..>2849)
                     /locus_tag="VN518_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006728468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidogalycan biosysnthesis protein"
                     /protein_id="HXE03347.1"
                     /translation="FIGADTLYGRYWGGVEDRPFLHFEVCYYQAIDFAIAHRLARVEA
                     GAQGAHKLARGYLPSETYSAHYIADPALRRAVADYLKRERRAVAREIALLGEESPYRR
                     GE"
BASE COUNT          522 a          913 c          942 g          472 t
ORIGIN      
        1 gaagaagcgc ggtgccgatg cgctcgagat ctattgcatc aatctgaatc aaaaggcccg
       61 cgccggccgc attgatccgc tgatcggccg cgggccggag atccagcgca ctatccaggt
      121 gctgtgccgc cggcagaaga acaacccgct gttcgtgggc gatcccggcg tgggcaagac
      181 cgccatcgcc gaaggcttgg cgcggcgcat cgtcatgggc gatgtgcccg aagtgctggc
      241 ggattcgacc atcttccagc tcgacatggg tgcgctgctg gcgggcaccc gctatcgcgg
      301 cgatttcgag gagcgactca aggcggtgat gcgcgagatc gaggcctatc ccggcgcgat
      361 catgttcatc gacgagatcc atacggtgat tggggcaggg gccacctccg gcggcgccat
      421 ggacgcctcg aacctgctga agccggcgct ccaatccggt acgctgcgat gcctcggctc
      481 gactacctac aaggaatatc gccagcattt cgaaaaggac cgggcgctgg tgcggcgctt
      541 ccagaagatc gacgtgaacg agccgtcggt gccggacgcg atcgagatcc tgaaggggct
      601 gaagccctat ttcgaggact tccaccgcgt ccgctatacc agccaggcga tcaagtcggc
      661 ggtcgagctg tcggccaagt acatccacga ccgcaagctg cccgacaagg cgatcgacgt
      721 gatcgacgag accggcgcct cgcagatgct ggtgcccgag cggcagcggc gcaagaccat
      781 cggcgtcaag gaggtcgagt cgaccatcgc caccatggcg cgcattccgc ccaagacggt
      841 gtcgaaatcc gacgccgagg tgctgcgcgc gatcgatcag gatctgaagc gggtcgtgtt
      901 cggccaggac aaggcgatcg aggcgctggc cgccgcgatc aagctgtcac gcgccggcct
      961 gcgcgagccc ggcaagccga tcggctgcta catgttttcc ggaccgaccg gcgtcggcaa
     1021 gaccgaggtc gcgcgccagc tcgcgctctt gctcggggtc gagctgctgc gcttcgacat
     1081 gagcgagtat atggagcggc ataccgtgtc gcgcctgatc ggcgcgcccc ccggctatgt
     1141 cggcttcgac caaggcggtc tgctcaccga cggcatcgac cagcacccgc actcggtgtt
     1201 gctgctggac gagatcgaga aggcgcatcc ggacctgttc aacatcctgt tgcaggtgat
     1261 ggatcacggc aagctgaccg accacaacgg caagcgcgtc gacttccaga acgtcatcct
     1321 catcatgacg accaacgccg gcgccagcga cctcgccaag gcggcgatcg gtttcaaccg
     1381 cgccaagcgc gagggcgacg acgaggaggc gatcaaccgc ctgttctcgc cggagttccg
     1441 caaccggctc gatgcggtca tcaccttcgc caatctcccg cccgaggtca tcgccaaggt
     1501 agtggagaag ttcatcttcc agctcgaggc ccaactcggc gaccgaaaca tcaccatcga
     1561 gctgacgccg gaggcgaatg cctggctggc cgagcgcggc tatgacgaca agttcggcgc
     1621 ccgcccgctg gcgcgcgtga tccaggagca catcaagaag ccgctggccg aggagatcct
     1681 gttcggtgcg ctgaaggatg gcggcatcgt acgcgtgctg gtcgaggaga aggccggaga
     1741 gaaggcgctc ggcttcgaat atctcaaagc cgacgcgccc gtgcgcccgc gcgatcccga
     1801 ggatatcgat cccgaaggcg aggggcccga tggcagcgga ccgaaaggcc caccgaaggc
     1861 gggcggaccc aagccgcggg tgcggcgcgg aggctcgcgc aagccgcggg cagcgcgcag
     1921 aagcgtcggc gccagcctcg tgcccaaggt ccctctcggc acgaagtagc cgcggtcagc
     1981 tcgccagttt cttgcgtatc ttctcggccg tagcggcgag ctccgcctga tcttccatct
     2041 tgccggcgtg cggcttgagc gcctcgccct gtctgcgcgg gacgatatgg acgtgcaggt
     2101 ggaacacggt ctggccggcg gcggcgccgt tgaattgctg gatcagcaca ccttcggcgc
     2161 ccattgcctc ttggatccgc ggcgtgagct tctgcaccac cgccatcagt ctggcgagcg
     2221 cctcaggctt gatatcgaac aggtcgcgcg ccttctcctt ggtgatgacc agtgcgtggc
     2281 cttccgcgcg cggcatgatg tcgaggaagg cgagcgtgtc ctcgtcctcg tagatcttgt
     2341 ggcaggggat ctcgccgcgc aggatcttgg cgaaaatatt gtccggatcg taggccatgc
     2401 tgtttctata gctgagtttt cgaaacgcgt cataccggcg aaagccggta tccaggaccc
     2461 attggctccg gtttgttgct ctggattccg gcttgcgccg gaatgacgcc gcgaacaagg
     2521 cgagcagact aactcactcc ccccgccgat aaggcgactc ttcgccgagc aacgcgattt
     2581 cacgggcgac ggcgcgccgc tcgcgcttga ggtaatcggc gacggcgcgg cgcagcgccg
     2641 ggtcggcgat atagtgtgcg ctataggtct cgctcggcag atagccgcgg gcgagcttat
     2701 gcgcgccttg cgcaccggcc tcgacgcgcg cgagtcggtg tgcaatggcg aagtcgatcg
     2761 cctgataata gcagacctcg aaatgcagaa agggacggtc ctcgacgccg ccccaatagc
     2821 ggccgtacag cgtgtcggcg ccgatgaag
//
LOCUS       DATNAE010000087         3991 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3103641, whole genome shotgun sequence.
ACCESSION   DATNAE010000087 DATNAE010000000
VERSION     DATNAE010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3103641"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1178)
                     /gene="nuoF"
                     /locus_tag="VN518_01860"
     CDS             complement(<1..1178)
                     /gene="nuoF"
                     /locus_tag="VN518_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HXE03348.1"
                     /translation="MLKDSDRIFKNLYGFHDWRLKAARARGAWDGTKAMIEKGHEAVI
                     EEVKASGLRGRGGAGFPTGLKWSFMPKQSDGRPNYLVINADESEPGTCKDRDIMRHDP
                     HLLLEGALLASFAMRAHVSYIYIRGEFIREREVLQGAIDEAYDAKLIGKNNSHGWDFD
                     LHLHHGAGAYICGEETALLESLEGKKGMPRLKPPFPANVGLYGAPTTVNNVESIAVVP
                     DILRRGATWFAALGKPNNTGTKLFCLSGHVNTPCNVEEEMGVDLRELIDRHAGGVRGG
                     WDNLLAVIPGGASVPLIPKSICDDVLMDFDSLKNVQSGLGTAAVIVMDKSTDVVKAIQ
                     RLAYFYKHESCGQCTPCREGTGWMWRVMTRMVKGQSHPEEIDLLYEVTKEVEGHTICA
                     L"
     gene            complement(1178..1933)
                     /gene="nuoE"
                     /locus_tag="VN518_01865"
     CDS             complement(1178..1933)
                     /gene="nuoE"
                     /locus_tag="VN518_01865"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HXE03349.1"
                     /translation="MSVRRLAAEQPEDFAFTPENLAWAKEQLKKYPQGREASAVLPLM
                     WRAQEQCGGWLPEPALRYIADLLDMAYIRVYEIATFYTMFNLAPVGRYYVQLCGTTPC
                     WLRGAEALKDTCRKLIGEPGHVTKDGMFSWIEVECLGACVNAPMVQINKDYYEDLTPE
                     SFEKILTDLREGRKVKPGPQDGRHSSEPAGGVTTLIGQGLYAEAPAREPHADEGELAL
                     IDDEAKKPGDAASEREAAVPKPPLRDSSGRRRK"
     gene            complement(1989..2453)
                     /locus_tag="VN518_01870"
     CDS             complement(1989..2453)
                     /locus_tag="VN518_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03350.1"
                     /translation="MGKVINVLQTLSATGAAVLLLVLALPLSAVAADKDASPEKGAPE
                     ASEPKDADKSKEEAKPDEAPPPDAKAEEAPPAGGGIQSGVKLKDILAEGFLIRTTVLV
                     PAEAVTRQIGKVSSDAVLLTLQKETAIAVCYYTLKAYVKEGLIDIASCTVFR"
     gene            complement(2485..3720)
                     /locus_tag="VN518_01875"
     CDS             complement(2485..3720)
                     /locus_tag="VN518_01875"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HXE03351.1"
                     /translation="MMAEIETRAQDSESETGQREGDDRKFTINFGPQHPAAHGVLRLV
                     LELDGEIVERVDPHIGLLHRGTEKLIEYKTYLQALPYFDRLDYVAPMNQEHAYSLAVE
                     KLVGVTVPKRAQLIRVLYSEIGRLLSHLLNVTTQALDVGALTPPLWGFEEREKLMVFY
                     ERASGSRMHAAYVRPGGVHQDLPPELVADIYAFCDPFLKVCDDIEGLLTGNRIFKQRN
                     VDIGVVKLEDAWAHGFSGVMVRGSGARWDLRKSQPYECYSELQFDIPIGKNGDCYDRY
                     LVRMEEMRQSVRIMKQCCERLTTELGPVTVDNHKMVPPKRAVMKGSMEALIHHFKLYT
                     EGYHVPAGEVYAAVEAPKGEFGVYLVADGTNRPYRCKIRAPGFAHLQAMDFLCRGHML
                     ADVSAVLGSIDIVFGEVDR"
     gene            complement(3717..>3991)
                     /locus_tag="VN518_01880"
     CDS             complement(3717..>3991)
                     /locus_tag="VN518_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HXE03352.1"
                     /translation="LLRPHLDPGEGSLGVHIDVSHKAATPVGFTITVDAECIEVRGPR
                     AKFAVRAHDGIDEIGSGIHERFIVSWDRFNKSVAAKAAKANAKVDA"
BASE COUNT          692 a         1257 c         1260 g          782 t
ORIGIN      
        1 agcgcgcaga tcgtgtggcc ttcgacttcc ttggtcacct catagagcag atcgatctct
       61 tcgggatgcg actggccctt caccatgcgc gtcatcacgc gccacatcca cccggtgccc
      121 tcgcggcagg gtgtgcattg gccgcagctc tcgtgcttgt agaaataggc gagccgctgg
      181 atcgccttca ccacgtcggt cgacttgtcc atgacgatca ccgcggcggt gccgaggccg
      241 gactgcacgt tcttcaagga gtcgaaatcc atcagcacgt cgtcgcagat cgatttcggg
      301 atcagcggca ccgaggcgcc gcccgggatc accgcgagca gattgtccca accgccgcgc
      361 acgcctccgg cgtggcggtc gatcagctcg cgcaaatcca cgcccatctc ttcctcgaca
      421 ttgcagggcg tgttcacgtg gcctgacagg caaaacagct tggtgccggt gttgttgggc
      481 ttgccgagcg ccgcgaacca ggtggcgcca cggcgcagga tgtcgggcac caccgcgatc
      541 gactcgacgt tattgaccgt ggtcggcgcc ccatagaggc cgacattggc ggggaacggc
      601 ggcttcagcc gcggcatgcc cttcttgcct tcgaggcttt cgagtagcgc cgtctcttcg
      661 ccgcagatat aggcgcctgc cccgtgatgc agatgaaggt cgaagtccca gccgtgtgag
      721 ttgttcttgc cgatcagctt cgcgtcatag gcctcgtcga tcgctccttg cagcacctcg
      781 cgctcgcgaa tgaactcgcc gcggatatag atgtaggaga catgcgcccg catggcgaag
      841 gacgcgagca gcgcgccttc gaggagaaga tgcggatcgt ggcgcatgat gtcccggtcc
      901 ttgcaggtgc cgggctccga ttcatcggca ttgatcacga ggtagttcgg gcggccgtcg
      961 gactgcttcg gcatgaacga ccatttgagg ccggtgggaa aacctgcgcc gccgcggcct
     1021 ctgagacccg aggccttcac ctcctcgatg acggcttcgt ggcccttctc gatcatggcc
     1081 ttggtgccgt cccaggcgcc ccgggcgcgt gccgccttga gccgccagtc atggaagccg
     1141 tagagattct tgaagatgcg gtcggagtct ttcagcatca ctttcgtctc cggccggagc
     1201 tgtcgcgaag aggaggcttc ggcacggcgg cctcgcgctc gcttgccgcg tcgccaggct
     1261 tctttgcctc atcgtcgatg agagcgagct caccctcgtc tgcatgaggc tcgcgcgccg
     1321 gcgcctcggc ataaagtcct tgcccgatga gcgtcgtcac tccgcccgcg ggctcggagg
     1381 aatgccggcc atcctgcggt ccaggcttca ctttccggcc ctcgcgcaag tcggtcagga
     1441 tcttttcgaa gctctccggc gtcaggtcct cgtaatagtc cttgttgatc tgcaccatcg
     1501 gcgcattgac gcaagcgccg aggcactcga cctcgatcca ggagaacatc ccatccttgg
     1561 tcacgtggcc aggctcgccg atcagcttgc ggcaggtgtc tttcagcgcc tcggcgccgc
     1621 gcagccaaca tggcgtggtg ccgcaaagct gcacgtagta gcggccaacc ggtgccaaat
     1681 tgaacatggt gtagaaggtg gcgatctcgt agacgcggat gtaagccata tcgagcaggt
     1741 cggcgatata gcggagcgcg ggctcgggca gccaaccgcc gcattgctcc tgcgcccgcc
     1801 acatgagcgg cagcaccgca gaggcctcac ggccttgcgg atatttcttg agctgctcct
     1861 tcgcccaagc gaggttctca ggcgtaaagg cgaagtcttc gggctgctcg gcggcgaggc
     1921 ggcggacgct cataggctcg cttcttcttt tggtttctgg atcgggcgca agagcgagac
     1981 aatgcctctt agcggaacac ggtgcaggag gcgatgtcga tcaggccctc cttcacatag
     2041 gccttgagcg tgtaatagca aaccgcgatc gccgtttctt tttgcagggt gagaaggacg
     2101 gcgtcggagg agactttgcc gatctgccgg gtcaccgcct cggcgggaac gagcaccgtg
     2161 gtcctgatca ggaagccttc ggcgagaatg tccttcaact tcaccccaga ctgaatcccg
     2221 ccgccggcgg gaggcgcttc ctcagccttc gcgtcaggcg ggggcgcttc atcgggcttc
     2281 gcctcctcct tggatttgtc ggcatccttc ggctcggagg cttcaggggc tcccttctcc
     2341 ggcgaagcgt ccttgtcggc tgcaacggcg ctgagcggga gggcgagaac caataggagg
     2401 acggccgcgc cggtggcgct caaagtctgc aacacgttga taactttacc catttcgtcc
     2461 ctcctccgat ctcgtagcca acgcctagcg gtcgacctcc ccgaacacga tatcgatcga
     2521 tcctaacact gccgagacat cggccagcat atggcctcgg cagaggaaat ccatggcctg
     2581 caaatgtgcg aagcccggcg ccctgatctt gcagcgatag gggcgattgg tgccgtcggc
     2641 gacaagatag acgccgaact cgcccttggg cgcctcgacc gcggcgtaaa cctcaccggc
     2701 aggcacgtga tagccctcgg tgtagagctt aaagtgatgg atcagagcct ccatcgagcc
     2761 tttcatcacg gcgcgcttcg gcggcaccat cttgtggtta tccaccgtca ccggcccaag
     2821 ctcagtggtc agtctttcgc aacattgctt catgatgcgc accgattggc gcatctcttc
     2881 catgcgcacg aggtaacggt cgtagcagtc gccgttcttg ccgatgggga tgtcgaactg
     2941 aagctccgaa tagcattcgt aaggctggct cttgcgcaaa tcccagcggg cgcctgagcc
     3001 gcgtaccatg acgcctgaga agccatgcgc ccaggcgtcc tcgagcttca cgacgccgat
     3061 atcgacattg cgctgcttga agatgcggtt gccggtgagc aggccctcga tgtcgtcgca
     3121 cactttgagg aaggggtcgc agaaggcgta gatgtcggcc acgagctctg gcggaagatc
     3181 ctggtgcacg ccgccgggac ggacgtaagc cgcatgcatg cggctgccgc tcgcccgctc
     3241 gtagaacacc atcagcttct cgcgctcctc aaagccccag agcggcggcg tcagcgcgcc
     3301 gacgtccaag gcttgcgtgg tgacgttgag gaggtgggag aggagccggc ctatctccga
     3361 atagagcacg cggatgagct gggcgcgctt cgggacggtg acgccgacga gcttctccac
     3421 cgcgagagag taggcgtgct cctggttcat cggcgccacg tagtcgaggc ggtcgaaata
     3481 aggcagcgcc tggagatagg tcttgtactc gatcagcttc tcggtgccgc gatggagcag
     3541 cccgatatgc ggatcgacgc gttcgacaat ctcgccgtcg agctcgagca cgaggcgcaa
     3601 cacgccgtgc gcggcagggt gctgcggccc gaaattgatg gtgaatttgc ggtcgtcgcc
     3661 ctcacgctga ccggtctcgg actcggaatc ctgagcgcgg gtttcgatct cagccatcat
     3721 gcgtcaacct tggcattggc ctttgcggcc ttggcggcga cggatttgtt gaagcggtcc
     3781 cagctgacga tgaaccgctc atggatgcct gagccgatct cgtcgatgcc gtcatgcgcc
     3841 ctcacggcga acttggcgcg tggccccctc acctcgatgc attcggcgtc gacagtgata
     3901 gtgaagccga ccggcgttgc ggccttgtgc gacacgtcga tatgcacgcc gaggctccct
     3961 tcgccggggt cgagatgggg acggagcagc t
//
LOCUS       DATNAE010000088         3923 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_696320, whole genome shotgun sequence.
ACCESSION   DATNAE010000088 DATNAE010000000
VERSION     DATNAE010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696320"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1262
                     /locus_tag="VN518_01885"
     CDS             <1..1262
                     /locus_tag="VN518_01885"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HXE03353.1"
                     /translation="KPIFEAAGLGSQAIDIHIVNDRAFNAFVIDGHNMFIHAGALMDA
                     KTPNQIIGVIAHESGHITGGHLARLRNQISRAKSAALMLQLLGLAAMAAGAVAGSGEI
                     GQLGMGAAYGGTDAAMRMVLAYRQDEESSADQAAVTFLNATHQSARGLLETLEFMNSK
                     LFGVQGINPYLQSHPLPPQRLAQLRSLAEASPYFDVKDPPELQVRHDLVKAKLFGFLD
                     QPDTTFNRYPSTDRSLPAAYARAIATYRKSGAAAAMPQLDALIAAKPDWPYFYEIKGQ
                     FLFESGNAADAIAPLQKAVALAPEESLIRILLAQSMLAMPGMKNVDDAIANLRTALAR
                     ETDSATGYRQLALAYGRKGDAAKAESARQQYLAQAELASAEAYFYEGRLKLAKEQAKR
                     AKAGFVDGTPNWLRADDILAFEVPTTD"
     gene            1290..2102
                     /locus_tag="VN518_01890"
     CDS             1290..2102
                     /locus_tag="VN518_01890"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HXE03354.1"
                     /translation="MNLRKVFFNVAVAAAVAAVTAAGTSYLVLRYAPAGKLPGASGPV
                     AADQARVEGILRDYLTKNPEILVEMTTELDKRQAEEQATQQKKAISDNADAIFRSPVA
                     HVAGNPSGDVSVVEFFDYNCGYCRRALPDVVKLVNGDGKIRLVLKELPIFGEDSEAAA
                     KLALASDKQGKYFEMHQKLFSEPGKSDKEKALRIAKELGLDVDKLQKDAEDPDIKKSL
                     DQAKELAQKLNLQGTPLYLIGDRVIPGAPDDLFDQLKKNVAEVREKGCASTC"
     gene            2206..2658
                     /gene="aroQ"
                     /locus_tag="VN518_01895"
     CDS             2206..2658
                     /gene="aroQ"
                     /locus_tag="VN518_01895"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HXE03355.1"
                     /translation="MIKPIYVLNGPNLNLLGSREPQVYGSETLEDVRARCERKAAKLG
                     FAIDFRQSNHEGELVAWIQEAKEKAAGLIVNAGAFTHSSIAMLDALLASELPCVEVHL
                     SNIFRRESFRHNSYISKGAKGVICGFGPLGYELAIEALAASLEPEKAR"
     gene            2655..3131
                     /locus_tag="VN518_01900"
     CDS             2655..3131
                     /locus_tag="VN518_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HXE03356.1"
                     /translation="MMAEERKAVTKKEGTFDKDLIRELAELLEETGLTEIEIEHEGKR
                     VRVARTVTVGAGASSIDAGRAPREAGERGAKEDHHPGTVHSPMVGTAYRSPEPGAPPF
                     VEAGTKVTAGQTILIIEAMKTMNHIPAPHAGTVLAVLVDDGQPVEYAEPLAVIERG"
     gene            3144..>3923
                     /locus_tag="VN518_01905"
     CDS             3144..>3923
                     /locus_tag="VN518_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HXE03357.1"
                     /translation="MFDKILIANRGEIALRIERACKELGIATVAVHSTADADAMHVRL
                     ADESVCIGGPPAKDSYLNIPQIVSACEITGADAVHPGYGFLAENARFAEILEEHEITF
                     IGPSAEHVRLMGNKIEAKVTAKSLGLPLVPGSDGPITSLDEAKKVASDIGYPVLVKAA
                     AGGGGRGMKVAHVEEELDAALATARIEAKTAFADDTVYLEKYLAHPRHIEVQVLGDGE
                     GNAIHLGERDCSIQRRHQKLWEEAPSPALNDGERAKIGETVA"
BASE COUNT          765 a         1283 c         1212 g          663 t
ORIGIN      
        1 ccaagccgat cttcgaggcc gccggcctcg gctcgcaggc catcgatatc cacatcgtca
       61 acgaccgcgc cttcaatgcc ttcgtcatcg acgggcacaa tatgttcatc catgccggcg
      121 cgctgatgga tgcgaagact cccaatcaga tcatcggcgt gattgcgcat gagagcggcc
      181 atatcaccgg cggtcatctc gccaggctga gaaaccaaat ctcgcgggcg aagtccgccg
      241 cgttgatgct ccagctgctc ggccttgccg ccatggcggc aggcgctgtc gcgggctcgg
      301 gcgagattgg gcaactcggc atgggcgccg cctacggcgg aaccgacgcc gcgatgcgca
      361 tggtgcttgc ctaccggcag gacgaagagt cctcggccga ccaggccgcc gtcacctttc
      421 tcaatgccac ccaccaatct gcacggggac tgctcgagac cttggagttc atgaactcca
      481 agctgttcgg cgtgcagggc atcaatccct acctgcaatc tcacccgctc cccccgcagc
      541 gtctcgccca gcttcgctcg ctggccgagg cgagcccgta tttcgacgtc aaggacccgc
      601 ccgagcttca ggtccggcat gacctggtga aggccaagct gttcggcttt ctcgatcagc
      661 cggatacgac gttcaaccgc tacccctcga cggatcgctc ccttcccgcg gcctatgcgc
      721 gcgccatcgc cacctatcgc aagtcggggg cggcggccgc gatgccccag ctcgacgccc
      781 tgatcgccgc caagcccgat tggccgtatt tctacgagat caagggtcag ttcctgttcg
      841 agagcggcaa tgccgccgac gccattgcgc cgctgcagaa ggcagtggcg cttgcgcccg
      901 aagagtcgct catccgcatc ttgctggccc agtcgatgct tgccatgccg ggcatgaaga
      961 acgtcgacga cgccattgca aatttgcgca ccgcgcttgc ccgcgagacg gactcggcga
     1021 ccggctaccg ccagctggcg ctggcttacg gccgcaaggg cgatgccgcc aaggcagaaa
     1081 gcgcgaggca gcaatatctg gcgcaagccg agcttgcgtc ggcggaggcc tatttctatg
     1141 aaggacggct gaaactcgcc aaggagcaag ccaagcgcgc caaggccgga ttcgtcgacg
     1201 gcacgccaaa ctggcttcgg gccgatgata tactcgcctt cgaggtgccg accaccgatt
     1261 gagctatttc cccccggaga accaaaaaca tgaacctcag aaaagtcttc ttcaatgtgg
     1321 ccgtggccgc cgcggtcgcc gccgtgacgg cggccggcac gagctatctc gtcctccgct
     1381 acgccccggc aggcaagctc cccggcgctt cggggccggt cgcggccgac caggcccgcg
     1441 tcgaaggcat cttgcgcgac tacctcacca agaaccccga gatcctcgtc gagatgacca
     1501 ccgagctcga caagcgccag gccgaagagc aggcgacgca gcagaaaaag gcgatctccg
     1561 acaatgccga cgcgatcttc cgctcgccgg tggcccacgt tgccggcaat cccagcgggg
     1621 atgtgagcgt ggtcgagttc ttcgactata actgcggata ttgccgccgc gcgctgccag
     1681 acgtggtcaa gctcgtcaac ggcgatggca agatccgcct ggtgctgaag gaattgccga
     1741 ttttcggtga ggattccgag gctgccgcca agcttgcact cgcttccgac aagcagggca
     1801 aatatttcga gatgcatcag aagctgttct cggagccggg caagtccgac aaagagaagg
     1861 ccttgcggat cgccaaggag cttgggctcg atgtcgacaa gctgcaaaag gacgccgagg
     1921 accccgacat caagaagtcg ctcgaccagg ccaaggagtt ggcccagaag ctcaatctgc
     1981 aaggcacgcc gctctatctg atcggggatc gcgtgatccc cggagcccct gacgatctct
     2041 tcgatcagct caagaagaac gtcgccgagg tcagggagaa gggctgcgca agcacctgtt
     2101 aacggtcgtc ctttgccggt aaccgggtgt ttccggcttt ttgctagacc caggaaaagc
     2161 tgttaaaggg gcgccggccg cttaccctcg cgcctgtctc acgccatgat caaaccgatc
     2221 tacgtcctga acggtcccaa tctgaacctg ctcgggtcgc gggagccgca ggtctatggc
     2281 agcgagacct tggaggacgt gcgggcgcgg tgcgagcgga aggcggccaa attgggcttt
     2341 gccatcgact tccggcagag caatcatgag ggcgagctgg tggcctggat tcaggaggca
     2401 aaggagaagg cggcggggct gatcgtcaat gccggcgcct tcacccatag ctcgatcgcc
     2461 atgctcgacg ctttgctcgc ctctgagctt ccttgcgtgg aggtgcatct gtccaacatc
     2521 ttccgccgcg agagctttcg ccacaattcc tatatttcca agggcgccaa aggcgtgatc
     2581 tgcgggttcg ggcctctcgg ctatgagctt gccatcgagg ccttggctgc ctcgcttgaa
     2641 cctgagaagg ctcgatgatg gccgaggagc gcaaggcggt gaccaaaaag gaagggacct
     2701 tcgacaagga cctcatccgc gagcttgccg agctactgga ggaaaccggt ctcaccgaga
     2761 tcgaaatcga gcatgaaggc aagcgcgtgc gcgttgctcg cacggtcacg gtcggagccg
     2821 gcgcatcctc catcgatgcc ggccgtgcgc cgcgcgaggc aggcgagcgg ggggccaaag
     2881 aggatcatca cccaggcaca gtgcactctc ccatggtcgg caccgcctat cgctcgccag
     2941 agccgggcgc cccgcctttc gtcgaggccg gcaccaaggt taccgcaggc cagaccatcc
     3001 tcatcatcga ggccatgaag accatgaacc acatcccggc cccccatgcc ggcaccgtgc
     3061 tggccgtcct cgtcgatgac ggccagcccg tcgaatatgc cgagccgctc gcggtcatcg
     3121 agaggggcta agcgcttctc ggcatgttcg acaaaatcct gatcgccaac cggggagaga
     3181 tcgcgcttcg catcgaacgt gcctgcaagg agctcggcat cgccaccgtt gcggtgcatt
     3241 cgaccgcaga cgccgacgcc atgcacgtgc gccttgccga tgagagcgtg tgcatcggcg
     3301 gtcctccggc gaaggacagc tatctcaaca ttcctcagat tgtgtcggct tgcgagatca
     3361 ccggcgcaga tgccgtgcat cccggctatg gcttcctcgc cgagaatgcc cgtttcgccg
     3421 agatcctcga agagcatgaa atcaccttca tcggaccgag tgctgagcat gtcaggctca
     3481 tgggcaacaa gatcgaggcc aaggtgaccg cgaagtcgct cggcctgccg ctcgtcccag
     3541 gctccgacgg gcccatcacc agcctcgatg aggcgaagaa ggtcgccagc gacattggct
     3601 atcctgtgct ggtcaaggct gcggcgggcg gcggcggccg cggcatgaag gtcgcccatg
     3661 tcgaggagga gctcgatgcg gcgctggcca cggcgcggat cgaggccaag accgccttcg
     3721 ccgacgacac cgtctatctc gagaaatatc ttgcccatcc ccgccacatc gaggtccagg
     3781 tgctcggcga cggcgagggc aacgccatcc atctcggtga gcgcgactgc tcgatccaga
     3841 ggcggcacca aaagctgtgg gaggaggcgc cctcgcctgc actgaacgac ggcgagcggg
     3901 ccaagatcgg cgagaccgtg gcg
//
LOCUS       DATNAE010000089         2172 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_1297736, whole genome shotgun sequence.
ACCESSION   DATNAE010000089 DATNAE010000000
VERSION     DATNAE010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1297736"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="VN518_01910"
     CDS             complement(<1..336)
                     /locus_tag="VN518_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HXE03358.1"
                     /translation="MPLKPPKHFLDIDALDSKTLRAIIDLAAEIKRKGRVPKSLKLKP
                     HPVLAMIFEKPSTRTRVSFEVAIHQLGGTSLALNPQDMQLGRGETLADTARVLSRYVD
                     AIMIRTGPLE"
     gene            complement(366..1553)
                     /locus_tag="VN518_01915"
     CDS             complement(366..1553)
                     /locus_tag="VN518_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HXE03359.1"
                     /translation="MSALLPTYARSELAFARGDGAYLFTEDGERYLDFASGVAVTALG
                     HAHPHLVKALSEQAKKLWHVSNLHRIPEQERLAARLTAATFADRVFFANSGAEAVECA
                     IKAARRYHFKHGAPERYRLVTFDGAFHGRTLATIAAGGQAKHLEGFGPPVDGFDQVAA
                     LDIDAVEAAIGETTAGILIEPIMGEGGMREVSWRFLQDLRALADEKGILLLLDEVQTG
                     VGRTGRLYAHEWAGITPDIMATAKGLGGGFPIGACLMTEAVGAAMTAGSHGSTFGGNP
                     LAMAVGNAVLDVVLEDGFLDHVANMGLRLKQRLAALKDEHGEIIEEVRGQGLMLGLKC
                     KLPNQKLLEALRARKMLSVQAGDNVVRLLPPLIIGEAEIELAATTLDAACVALKRDVL
                     THA"
     gene            1939..>2172
                     /locus_tag="VN518_01920"
     CDS             1939..>2172
                     /locus_tag="VN518_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GcrA family cell cycle regulator"
                     /protein_id="HXE03360.1"
                     /translation="MAWTDERVELLKKLWAEGLSASQIAGRLGGVTRNAVIGKVHRLG
                     LSGRATSSRSSSPRPRRTHVPRANRTPSLMFGTR"
BASE COUNT          386 a          681 c          711 g          394 t
ORIGIN      
        1 ttccaacggt ccggtgcgga tcatgatcgc atcgacataa cgcgacagca cgcgcgccgt
       61 atcggccagc gtctcgcccc ggccgagctg catatcctgc gggttgagcg cgagcgaggt
      121 gccgccgagc tgatggatcg ccacctcgaa ggagacgcgc gtgcgggtcg acggcttctc
      181 gaagatcatg gcaagcaccg gatgcggctt gagcttcaga ctcttcggca cgcgaccctt
      241 gcgcttgatc tccgcggcga gatcgatgat agcgcgcagc gtcttgctgt ccagtgcgtc
      301 gatatcgagg aaatgcttgg gcggctttag gggcatggcg gtggcgctgt gcgtgagatc
      361 ggcgcctaag cgtgggtcag cacgtcacgc ttgagcgcga cgcaagcggc atcgagggtg
      421 gtcgcggcga gctcgatctc cgcctcgccg atgatcaacg gcggcagcag gcgcacgacg
      481 ttatcgccgg cttgcacgct cagcatcttc ctcgcgcgca aggcctccaa cagcttctgg
      541 ttgggcagct tgcatttcaa tccgagcatc agcccctgcc cgcgcacctc ttcgatgatc
      601 tcgccatgct cgtccttcag cgcggcgaga cgctgcttca accttagccc catgtttgcg
      661 acatgatcga gaaagccgtc ctcgagcacg acgtcgagca cggcattgcc gaccgccatg
      721 gccagcgggt tgccgccgaa ggtcgagccg tggctgcccg cggtcatggc cgctccgacc
      781 gcctcggtca tgagacaggc gccgatagga aagccgccgc ccaagccctt ggcggtggcc
      841 atgatgtcgg gcgtgatgcc ggcccattcg tgcgcgtaga gccgtccggt gcggcccacg
      901 ccggtctgca cctcatccag cagcagcaga atccccttct cgtcggcgag cgcgcgcaaa
      961 tcctggagga accgccagga gacttcccgc atgcccccct cgcccatgat cggctcgatg
     1021 aggattccgg ccgtggtctc gccgatcgcc gcctcgaccg catcgatatc gagcgcggcc
     1081 acctggtcga agccgtcgac cggcgggccg aagccttcga gatgctttgc ctggccgcca
     1141 gcggcgatgg tggcgagcgt cctgccatga aaggcgccgt cgaaggtgac gagccgatag
     1201 cgctcgggcg cgccatgctt gaaatgatag cgccgcgcgg ccttgatcgc gcattcgacc
     1261 gcctcggcac cggaattggc gaagaacacc ctgtcggcga aggttgcggc ggtgagccgt
     1321 gcagccaatc tctcctgctc cgggatgcga tggagattgg agacgtgcca gagctttttc
     1381 gcctgctccg acagggcttt cacgagatgc ggatgcgcgt gtccgagcgc ggtgacggcc
     1441 acgccgctcg cgaagtcgag atagcgctcg ccgtcctcgg tgaagagata ggcgccgtcc
     1501 ccgcgggcga aggcaagctc gctgcgggca taggtcggaa ggagggccga catcggatct
     1561 tcgctgtcct gaaataaaat tgccgccaac cggcggcagc aaacaatctc agagcattag
     1621 gtctccggtc ggagcgtgtc aatcagggca catgagggct ttatccaacg ctcgtccacg
     1681 tttccataca cgcatcgatt tgtgttcttg cggcagaagt ggcagaatcc accttgttcc
     1741 ggtggaaaac ggacagatta agcgtggcgg acgcttgccg taattttccg gatatagtag
     1801 cgtattaccg ctcggctaca agatgtcgtg ggtactgcgt cgcgcccggg cggtcaaacc
     1861 cagcagtaat tccgagtttt caaacgccgt ctctaggcag acggggggat tccgagtcca
     1921 attcgaggag gccaaaccat ggcttggact gacgagaggg ttgaactgct taagaaactc
     1981 tgggccgagg gcttgagtgc gagtcagatt gcgggccgct tgggcggcgt gacgcgcaac
     2041 gcggtcatcg gcaaggtgca tcgtctgggt ctgtcggggc gagccaccag ctcgcgttcg
     2101 tcgagcccga gaccgcggcg gacgcatgtg ccgcgcgcca accgcacgcc gtccctgatg
     2161 ttcgggacgc gc
//
LOCUS       DATNAE010000090         8293 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_1901795, whole genome shotgun sequence.
ACCESSION   DATNAE010000090 DATNAE010000000
VERSION     DATNAE010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 8293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8293
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1901795"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="VN518_01925"
     CDS             <1..543
                     /locus_tag="VN518_01925"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HXE03361.1"
                     /translation="KLSDPDLARGGAAVTAIRIENFTSAARYRVGRLKDRLAAYSPAV
                     ELDNARSRAFWEEIRTLRAFAGSDRPLWRISTIPSRAAKLVAIVARTLDIRVAYDWSG
                     GLIWLETPPTSDAGTVEIRRAIAEFGGHATLIRAERATRASVEVFQPLDPPLAALTAK
                     LKAAFDPAGILNPGRMYPGV"
     gene            552..1901
                     /gene="glcF"
                     /locus_tag="VN518_01930"
     CDS             552..1901
                     /gene="glcF"
                     /locus_tag="VN518_01930"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003597923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HXE03362.1"
                     /translation="MHTNFSHEQLQDPTIARADAMLKSCVHCGFCTATCPTYVLLGDE
                     RDSPRGRIYLIKEMFEQGAVTAPITHHIDRCLSCLSCMTTCPSGVDYMHLVDLARVRI
                     EQKGRSPGQRVTRWLLAKVLPSPGRFRIALLFGWLARPFRGLISALGMKTGAAALALV
                     PSRLPPRRTPKHEPVVRPAGEPVKRVALLLGCVQEVLQPSINRAAISLLKRHGVEVMM
                     PSGEACCGALQHHLGRENETQKAARRNVDAWSSALREGRLDAILTTASGCGTMVKDYG
                     HLLARDRGYAERATEMSRLTRDITEFIYELGLLPPLAWTSLRVAYHAACSLEHGQQVG
                     NEPRALLIQAGYTVLDVPESHICCGSAGTYNLLQPELAGQLRERKLKNVASVKPDLIA
                     TGNIGCITQLEHGSGVPVVHTIELLDWATGGPCPKPLEKLKDKVHPIESLMQLAGAR"
     gene            2013..2648
                     /locus_tag="VN518_01935"
     CDS             2013..2648
                     /locus_tag="VN518_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03363.1"
                     /translation="MSKQERPREQISGIVGGHIVPATETPLLTSASTPWSGFLLEKHN
                     AGDRQDVNWGWHRAHISLITKGQLGFSVRTSRGNQDFVGQAGSVCVFPSGYDETHFAI
                     AGSKFEAIVVELDPTRLETLLERPASASTDTLAPQIVVRDRYMAMLLRAMASEVAQGC
                     PAGALYGQALSLALATYLERRFAVKGRAAKKKHLRLVWAGRPSLAGRTPMG"
     gene            2689..4224
                     /gene="hisS"
                     /locus_tag="VN518_01940"
     CDS             2689..4224
                     /gene="hisS"
                     /locus_tag="VN518_01940"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003609123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HXE03364.1"
                     /translation="MNRQDEPVRPEARLPKGLFDTPASELRACADMLAKIREVFERYG
                     FEPLDTPAIEYTDALGKFLPDQDRPNEGVFSFKDDDEQWLSLRYDLTAPLARYVAENY
                     QNLPKPFRRYQVGPVWRNEKPGPGRFRQFTQFDADTVGASSVAADAELCMLAADTLEA
                     LGVPRGDFIVKFNSRKLLDGVMEEVEVTDERQQLAVLRAVDKLDRLGPEGVRGLLGKG
                     RKDESGDFTKGAELTERQAKEILQFIDIVPVESGDPTQPKWGRIQSGVSPDDPEQQFR
                     WLDLRMKSSIGRDGVTELKEIAALITAAGYQDRIRVDPTVVRGLAYYTGPVFEAELLA
                     PIKDERGQSIRLGSVVSGGRYDGLVERFTGEKVPATGVSIGVSRLLYGMKTLGKIPAE
                     TVQGPVVVLVLDRDRIADYQKMAQALRNTGVRAELYLGESGMKAQMKYADKRGSPCVV
                     IQGGDEKAKGELQIKDLVLGATLTSIKDRDDYLKKQAEAQFAVKESELVTEVRKVLAR
                     HKA"
     gene            4228..5400
                     /locus_tag="VN518_01945"
     CDS             4228..5400
                     /locus_tag="VN518_01945"
                     /inference="COORDINATES: protein motif:HMM:NF024785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HXE03365.1"
                     /translation="MAAESARKFEALEAQAATLLEIFRRGGYEQVAPSIIQPADVLLD
                     QIGEAIRGRTYVFTDLSGEELCLRPDLTVPVSRLYLERHPRADAPARYCYNGPAFRFQ
                     PKGASGTHPREFRQAGIECFGVADRDSADAEVVLLVADAVKSAGLKQFRFRFGDIALF
                     YALLDALALPERWRLKLRHYFWRPPAFHALLSRLAKGEAPNSDGPLAEFAATLADKDQ
                     ARAEELVANYLETNGLPLSGNRTLGEITERLLDHGADLRSEPLPREVATVIDYYLAVE
                     ASPREAIDRIAMIAKGAGIDLGPALYMLSRRFDLLQERGIDIGAARFATEFGRDLEYY
                     SGLVFQIETAGDRASPIAGGGRYDGLLRSVGAPREVPAVGSAIHTERLLAAVEGES"
     gene            5400..6104
                     /gene="hisG"
                     /locus_tag="VN518_01950"
     CDS             5400..6104
                     /gene="hisG"
                     /locus_tag="VN518_01950"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HXE03366.1"
                     /translation="MAATLTIAVPSKGRLKDNADALFERAGLALRKTGHERGYSGVMQ
                     GLDGVEVAFLSAAEIVHQLESGRVHMGVTGEDLVRETLPDVETSVEFLAPLGFGRADV
                     VVAVPDCWIDVARMEDLEDVAAQFARAHGRPLRVATKYLNLTRRFFAKAGVAGYRIVE
                     SVGATEGTPAAGTSELIVDITTTGTTLKANHLKVLADGLILKSQAHLFAASGADWTEG
                     ARGLRDAILDRLSKAV"
     gene            6115..6834
                     /locus_tag="VN518_01955"
     CDS             6115..6834
                     /locus_tag="VN518_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HXE03367.1"
                     /translation="MTMIARLALAVLLIWCAVAPAFATPARIIILRHGEKAGAWKLCG
                     VGQARADALAATYLGRDAAQSLFAAGEEPAAFLAITLHTLELAAPAAATWDLPVTMFS
                     VLPQKATEAFDKAANLRTEEAAAALLANPAWQGKTVVMVWEHKHIANAKLAAKSSGEA
                     VTLRQLLQLDKLKDVPETWPAANYDYFWIVDYAGGSTTPTGFRMVKQAFGGRYAKLPT
                     NDWGAPNGLTSDSECDLKGAE"
     gene            complement(6844..>8293)
                     /locus_tag="VN518_01960"
     CDS             complement(6844..>8293)
                     /locus_tag="VN518_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HXE03368.1"
                     /translation="WLGDGVHYRMRLKGDSADNPDQRIADDIKLFINSTLDIGIALLG
                     SFVTLISFVVILWGLSSSFPLVIGSQSFNIPGYLVWAALIYAILGTWVTHLVGRPLIK
                     LNFDQQRYEADFRFSLVRLRENAEEVTLLAGEPAEKERLLDRFGRVVGNWYGIMQRTK
                     RLTFLTAGYSQIAIIFPFIVVSPLYFAGSMMLGGLMQIASAFGQVQGALSFFVKAYSD
                     IADWKAVLNRLAGFDASMDWAKGLDTEAPRVQLVSDGGTAMAAEELTVSLPTGEEIVH
                     ASGLAIGPAERVLVTGPSGSGKTSLFRALGGVWPFGAGTIRIPQGAKLLVLPQRSYLP
                     LGTLRGALAYPGPDDAFTPEEIDEVLDATGLSHLREALDETAYWADRLSMGEQQRLSI
                     ARALLQKPDWLFLDEATAALDEPAEGMLYRLMLERLPEAAIVSIGHRSSLVVFHERFL
                     ALTPDGKGRHRLTAVSPDEGARDAEEMGLSLV"
BASE COUNT         1483 a         2815 c         2607 g         1388 t
ORIGIN      
        1 aagctgtccg acccggatct ggcaagaggc ggcgccgcgg ttaccgccat caggatcgaa
       61 aattttacgt ctgccgcgcg ctaccgcgtc gggcgactga aggatcggct cgccgcctat
      121 tcaccggcgg tcgagctcga caatgcgcgg agcagggcct tctgggaaga gatcaggacc
      181 ttgcgcgcct ttgccggctc tgaccgtccg ctgtggcgca tctccaccat cccatcgcgc
      241 gccgccaagc tcgttgccat cgtcgcccgc accctagaca tccgcgtcgc ttatgattgg
      301 tctggcggtc tgatctggct cgagacgccg ccgacctccg acgccggcac cgtcgagatc
      361 cgccgcgcca tcgccgaatt cggcggtcat gccacgctga tccgcgccga gcgggcgacc
      421 cgggcaagcg tcgaggtgtt ccagcccctc gacccgccgc tcgcggcctt gaccgctaag
      481 ttgaaagcag ccttcgatcc agccggcatt ctcaatccag gccgcatgta tcccggagtc
      541 taggcgccca catgcacacc aatttctccc acgagcagct ccaggacccg accatcgccc
      601 gcgccgacgc catgctcaag agctgcgtcc attgcggctt ctgcaccgcc acctgcccga
      661 cctatgtgct gctcggcgat gagcgcgatt ccccgcgcgg ccgcatctac ctgatcaagg
      721 agatgttcga gcagggcgcg gtcaccgcgc cgatcaccca ccatatcgac cggtgtctct
      781 cctgcctctc ctgcatgacc acctgcccga gcggggtgga ctacatgcat ctcgtcgatc
      841 tcgcgcgcgt ccgcatcgag caaaaagggc gctcgccggg acaacgcgtc acgcgctggc
      901 ttcttgccaa ggtgctgccg agcccaggcc gcttccgcat cgcgctgctt tttggctggc
      961 tcgcacgtcc gttccgcggg ctgatatcgg cgctcggcat gaagaccggc gccgccgcgc
     1021 tcgcgctcgt gccatcgcgc ttaccgcctc ggagaacgcc gaagcacgag ccggtcgtcc
     1081 gcccggcggg agagccggtc aagcgcgtgg cgctgctgct cggctgcgtg caggaggtgt
     1141 tgcagccctc catcaaccgc gccgccatca gcctgctcaa gcgccatggc gtcgaggtga
     1201 tgatgccgag cggggaggcc tgctgtggcg cgctgcagca ccatctcggc cgcgagaacg
     1261 agacgcaaaa ggcggcgcgg cgcaacgtcg atgcctggtc gagcgcgctc cgcgaaggcc
     1321 gcctcgacgc cattctcacc accgcttcag gctgcggtac catggtcaag gattacggcc
     1381 atctgctcgc ccgcgaccgc ggctatgccg agcgcgccac tgagatgtcg aggcttaccc
     1441 gcgacatcac cgagttcatc tatgagcttg gcctgctccc gccgctcgcc tggaccagcc
     1501 tcagagtcgc ctatcacgcg gcctgctcgc tcgagcatgg ccagcaggtc ggcaacgagc
     1561 cccgcgcgct gctcatccaa gccggctaca ccgtgctcga cgttcccgag agccatattt
     1621 gctgcggctc tgccggaacc tacaatcttc tccagccgga gcttgccggc caattgcgcg
     1681 agcggaagct caagaacgtc gccagcgtga agcctgacct gatcgccacc ggcaatatcg
     1741 gctgcatcac ccagctggag cacggcagcg gcgtgccggt ggtgcacacc atcgagctgc
     1801 tcgactgggc gaccggcggc ccgtgcccga agccgctgga gaagctcaag gacaaggtgc
     1861 acccgatcga gagcctgatg cagctcgccg gcgcccggta acggctagcg gtccaaccgt
     1921 cacgacgtac aacctattga tttgcaaccc ccctccaggt gcaactatac tgtcatagac
     1981 tgaagacggg gaggctccac gcagactcgt tcatgtccaa gcaagagaga ccgagggagc
     2041 aaatcagcgg gatcgtcggc gggcatatcg tccccgcgac cgaaacgccg cttctgacca
     2101 gcgcctctac cccttggtcc ggcttcctgc tggaaaagca caatgccggc gaccgacaag
     2161 acgtcaactg gggatggcat cgggcacata tcagtcttat taccaagggc cagctcggct
     2221 tcagcgtccg cacctcccgt ggcaatcagg atttcgtggg gcaggccggg agcgtttgcg
     2281 tgttcccgag cggctatgac gagacgcatt ttgcgatcgc cggatccaag ttcgaggcga
     2341 ttgtcgtaga gcttgatcca actcggcttg agacattgtt ggagcggccg gcctcggcgt
     2401 caaccgacac gctggccccg cagatcgtgg tacgagatcg ctatatggcg atgctgctca
     2461 gggcaatggc atcagaggtt gctcagggct gtcccgccgg tgccctctac gggcaggctt
     2521 tatctctggc gttggcgact tatctcgagc gaagatttgc ggtgaagggc cgcgccgcga
     2581 agaaaaaaca cctgaggttg gtctgggccg gcagaccgtc gcttgctggg cgcaccccta
     2641 tgggctagaa gccgcgaagc cagagccaag ccgtcaaccg ccaatcccat gaacagacaa
     2701 gacgagccgg tccgccccga ggcccgcctg ccgaagggcc ttttcgacac gcccgcgagc
     2761 gagcttcgcg cgtgcgccga catgctcgcc aagatcaggg aggtattcga gcgctacggc
     2821 ttcgagcctc tcgatacgcc ggccatcgaa tataccgacg cgctcggcaa gttcctcccc
     2881 gaccaggacc ggccgaacga gggcgtgttc tccttcaagg acgacgacga gcagtggctg
     2941 tcgctccgct atgacctcac cgcgccgctc gcccgctacg tcgccgagaa ctaccagaac
     3001 ctgccgaagc ccttccgccg ttaccaggtc ggcccagtgt ggcggaacga gaagccgggg
     3061 cctggacgct tccgccaatt cacccagttc gatgccgaca cggtcggcgc gtcctccgtc
     3121 gccgccgacg ccgagctttg catgctcgcc gccgatacgc tcgaagcgct cggcgtcccg
     3181 cgcggcgact tcattgtgaa attcaacagt cgcaaactac tagatggcgt catggaagaa
     3241 gtcgaagtca ccgacgagcg tcagcaactc gcggttcttc gcgccgttga taagcttgat
     3301 cgcctgggtc ctgaaggtgt tcgcggatta ctggggaaag gacgcaagga cgaaagcggc
     3361 gacttcacga agggtgcgga gctaaccgag cgacaggcga aggaaatcct gcagttcatc
     3421 gatatcgtgc ctgtggaatc cggcgatccg acacaaccca aatggggccg tattcaaagc
     3481 ggcgtaagtc cagatgaccc tgagcaacag ttcaggtggc tcgatcttcg gatgaagtcg
     3541 tcaatcggtc gagatggagt aaccgaactc aaggaaatcg cagccctgat cactgccgct
     3601 ggctaccaag atcgcatacg agtcgacccg accgtcgtgc gcggcctcgc atattacacc
     3661 ggtcctgtgt tcgaggccga gcttctcgcg ccgatcaaag acgagcgcgg gcaatcgatc
     3721 cgcctcggct cggtggtgag cggcgggcgc tatgacgggc tggttgagcg cttcaccggc
     3781 gagaaggttc ctgccaccgg agtctccatc ggcgtctcgc ggctgctgta tgggatgaag
     3841 actctcggca agatccccgc cgagaccgtg caaggccccg tcgtggtgct ggtgctcgat
     3901 cgcgaccgca tcgccgacta tcagaaaatg gcgcaagcct tgcgcaacac aggtgtccgc
     3961 gccgagcttt atctcggcga atccggcatg aaggcgcaga tgaaatatgc cgacaagcgc
     4021 ggcagccctt gcgtcgtcat ccagggcggc gacgagaagg cgaagggcga gttgcagatc
     4081 aaggatctcg tcctcggcgc cacgctcacc tcgatcaagg accgcgacga ctatttgaag
     4141 aaacaggccg aggctcagtt cgccgtgaag gagagcgagc ttgtcaccga ggtgaggaaa
     4201 gtcctcgccc gccacaaggc ctgacccatg gccgctgaat cggcgcgaaa attcgaggct
     4261 ctcgaagccc aggccgccac cttgctcgaa attttccggc gaggcggcta cgagcaggtt
     4321 gcgccttcca ttatccagcc cgccgatgtc ctgctcgatc agatcggcga ggccatcaga
     4381 ggccgcacct acgtcttcac cgacctttcc ggcgaggagt tgtgcctcag gccagacctc
     4441 accgtgcctg tgtctcgcct ttatctcgag cgtcatccgc gcgccgatgc gccggcccgc
     4501 tattgctata acggccccgc cttccgcttt cagccgaagg gcgcgagcgg aacgcatccg
     4561 cgcgagttcc gccaggccgg catcgaatgc ttcggcgtcg ccgaccgcga cagcgccgat
     4621 gccgaggtcg tgctgctcgt cgccgacgcg gtgaagagcg ctggcctgaa gcagttccgc
     4681 ttccgcttcg gcgacattgc cctgttctac gcgctgctcg atgcgctggc gctgcccgag
     4741 cgctggcggc tcaagcttcg tcactatttc tggcggccgc cagccttcca cgcgctgttg
     4801 tcgcgcctcg ccaagggcga agccccaaac agcgacggcc cgctcgcaga gttcgccgcc
     4861 acgcttgccg ataaggatca ggcccgcgcc gaggagttgg tcgccaatta tttggagacc
     4921 aacggcctgc ctttgtcggg caaccgcacg ctcggcgaga tcaccgagcg cctgctcgac
     4981 catggcgccg acctccgctc cgagccgctg ccgcgcgagg tggcgaccgt catcgactat
     5041 tacctcgcgg tcgaagcgtc cccgcgcgag gcgattgacc gcatcgccat gatcgccaag
     5101 ggcgccggca tcgaccttgg gcccgcgctc tacatgctgt cccgccgctt cgacctgctg
     5161 caggagcgcg gcatcgacat cggcgccgcg cgcttcgcca ccgagttcgg ccgcgacctc
     5221 gaatattatt cgggcctcgt gtttcagatc gaaactgccg gcgacagagc gagcccgatc
     5281 gccggcggcg gccgctatga cgggctgctc cgctcggtcg gcgctccccg cgaggtccca
     5341 gcggtcggct ccgccatcca caccgagcga ctgcttgccg cggtcgaggg ggagtcgtga
     5401 tggcggcgac cctcaccatc gccgtcccct ccaagggccg cctcaaggac aacgccgatg
     5461 ctctgttcga gcgcgctggc ctcgccttgc gcaagaccgg gcatgagcgc ggctatagcg
     5521 gcgtgatgca agggctcgac ggcgtcgagg ttgccttcct ctccgcagca gagatcgtgc
     5581 atcagctcga gtcgggccgc gtgcacatgg gcgtgaccgg cgaggatctg gtccgcgaga
     5641 ctcttcccga tgtcgagaca agcgtggagt tcttggcacc cctcggcttc ggccgcgccg
     5701 atgtcgtcgt ggcggtgccg gattgctgga tcgatgtcgc ccgcatggaa gacctcgagg
     5761 acgttgccgc gcagttcgcc agggcgcatg gcaggccgct ccgcgtcgcc accaaatatc
     5821 tgaacctcac ccgccgcttt ttcgccaagg ccggcgttgc cggctaccgc attgtggaaa
     5881 gcgtcggcgc caccgaaggc acgcccgccg ccggcacctc cgagctcatt gtcgatatca
     5941 ccaccaccgg cactacgctc aaggccaatc atttgaaggt gcttgcggac ggcctgatcc
     6001 tgaagtcgca ggcccatctc ttcgccgcaa gcggcgccga ctggaccgaa ggcgcgcggg
     6061 gcttgcgcga cgccattctc gaccgtctct ccaaggcggt ctaaaggagg gggcttgacc
     6121 atgatcgccc gcctcgccct tgccgttctc ctgatctggt gcgccgttgc cccagccttt
     6181 gccacgcccg cgcgcatcat catcctccgc cacggcgaga aagcaggtgc ctggaagctt
     6241 tgcggcgtcg gtcaggcgcg ggccgatgcg ctcgcagcca cctatctcgg ccgcgacgcc
     6301 gcccagtcgc tgttcgccgc cggcgaagag ccggccgcct tcctcgccat caccctgcac
     6361 acgctcgagc ttgccgcgcc tgccgccgcg acctgggatc taccggtcac catgttctcc
     6421 gtgctgccgc agaaagccac ggaggccttc gacaaagcgg ccaaccttcg tactgaggag
     6481 gccgctgcgg cgctgctcgc caatccggcc tggcagggca agaccgtggt gatggtgtgg
     6541 gagcacaagc acatcgccaa tgccaagctc gcggcgaaat cctccggcga ggcggtaacg
     6601 cttcgccagc tcctgcagct cgacaagctc aaagacgtgc cggaaacctg gccagcagcg
     6661 aactacgact atttctggat cgtcgactac gccggcggtt ccacgacgcc gaccggcttc
     6721 cgcatggtga agcaggcgtt cggcgggcgt tacgcgaagc tccccaccaa tgactggggc
     6781 gctccgaacg ggctcacgtc tgacagcgag tgcgatctga aaggggctga gtgaaggcct
     6841 gcctcagacg aggctgagtc ccatctcctc ggcgtcgcga gcgccttcgt cgggagagac
     6901 agcggtcagc ctgtgccggc ctttaccgtc aggcgtcagc gcgaggaagc gctcgtggaa
     6961 caccacgagg ctcgagcggt ggccgatcga cacgatggcg gcttcaggca ggcgctcgag
     7021 catcaggcga tagagcatgc cctcggcagg ctcatcgagc gcggccgtcg cctcgtcgag
     7081 aaacagccag tccggcttct gtagcagcgc gcgcgcgatc gacagccgct gctgctcgcc
     7141 catggagagc cggtcggccc aataggcggt ttcgtcgaga gcctcgcgca aatgcgaaag
     7201 cccggtcgcg tccaacacct cgtcgatctc ctcgggcgtg aaggcgtcgt caggccctgg
     7261 ataggcaagc gccccgcgca acgtcccgag cggcagataa gacctctgcg gcaagaccag
     7321 gagtttcgcg ccttgcggga tgcggatggt gccggcgccg aacggccaca ccccgccgag
     7381 tgccctgaac aggctcgtct tgcccgatcc cgacgggccg gtgaccagca cgcgttcggc
     7441 aggcccgatg gcgagacccg aagcgtgcac gatctcctcg ccggtcggca ggctgacggt
     7501 gagctcctcg gcggccatgg ccgtgccgcc gtcgctcacg agctggaccc gcggcgcctc
     7561 ggtgtcgagc cccttggccc agtccatcga ggcgtcgaag ccagcaagcc ggttcagcac
     7621 cgccttccaa tcggcgatgt cggagtatgc cttgacgaag aacgacagcg cgccctgcac
     7681 ctggccgaag gccgaggcga tctgcatcaa tccgccgagc atcatcgagc cggcgaaata
     7741 aagcgggctc accacgatga acgggaagat gatggcgatc tgcgaatagc ctgcggtgag
     7801 gaaggtcagc cgcttggtgc gctgcatgat gccgtaccaa ttgccgacca cgcggccgaa
     7861 gcggtcgagc aggcgctcct tctcggcggg ctcgccggcg agcagcgtca cttcctcggc
     7921 attctcgcgc aagcgcacga gcgagaagcg gaagtctgct tcatagcgct gctggtcgaa
     7981 attgagcttg atcagcggac gaccgacgag atgcgtcacc caggtgccga ggatggcgta
     8041 gatcagcgcc gcccagacga ggtagccagg gatgttgaag gactgcgatc cgatcacgag
     8101 cgggaagctc gacgacaggc cccacaggat caccacgaag gagatgagcg tgacgaagga
     8161 gccgagcagc gcaatgccga tgtcgagggt cgagttgatg aacagcttga tgtcgtcggc
     8221 gatgcgctgg tcggggttgt cggcggagtc gcccttgagc cgcatgcggt agtgcacgcc
     8281 gtcgccgagc cag
//
LOCUS       DATNAE010000091         2686 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_1299007, whole genome shotgun sequence.
ACCESSION   DATNAE010000091 DATNAE010000000
VERSION     DATNAE010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1299007"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..1088
                     /locus_tag="VN518_01965"
     CDS             54..1088
                     /locus_tag="VN518_01965"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HXE03369.1"
                     /translation="MRPRNGIIEAIGNTPLIKLKRASEETGCTILGKAEFMNPGQSVK
                     DRAALFIIRDALNSGRLQPGGTVVEGTAGNTGIGLALVGNALGLKTVIVIPETQSDEK
                     KDMLRLAGARLIEVPAVPYKDPNNYVKYSGRLAEELASSEPNGAIWANQFDNVANRQG
                     HIETTAQEIWKELGGKVDGFICAVGTGGTLAGVSMGLKEHNKDVVIGLADPMGAALYN
                     YYKHGVLKSEGSSITEGIGQGRITANLEGAKIDEAYQIPDAEALPVIFDLLSEEGLCL
                     GGSSGINVAGAIRLARDLGPGHTIVTVLCDYGTRYQSRLFNPKFLREKGLPVPAWLDQ
                     GQAAAPRVFV"
     gene            1110..1961
                     /gene="sseA"
                     /locus_tag="VN518_01970"
     CDS             1110..1961
                     /gene="sseA"
                     /locus_tag="VN518_01970"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HXE03370.1"
                     /translation="MLQTKTWLTETDELARELDAPDLVIIDASWHMPAENRNAHEEYL
                     AEHIPGAIFFDIDEIADTKSGLPHMLPPPEKFSSRMRSMGIGDGSRIVVYDSSGLFSA
                     ARVWWTFRVMGVDDVSVLNGGLPKWKREGLPLESGPARSRTARHFTARRNLDLVRDVG
                     DIKALLKDRSAEIVDARAADRFAGKAPEPRPGLRSGRIPGSHNLPFAKLLNKDGTLKT
                     APEIERLFEEAGVDLSKPVVASCGSGITASVLALGLAEIGHRRAAVYDGSWSEWGADQ
                     NLPIETG"
     gene            1997..>2686
                     /locus_tag="VN518_01975"
     CDS             1997..>2686
                     /locus_tag="VN518_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXE03371.1"
                     /translation="MSKRSRTQKDLTRLVHAGREPARHHGFVNTPIYRGSTILFPTVE
                     ALEANAQDFTYGRLGTPTVRALEEAIAELEGGQRTLLTPSGLSAIATSLLAFLAAGDE
                     VLVSDSVYRPTRRFCDGVLKRLGVKTIYYDPLIGAGIEKLITKKTKVVFTESPGSQTF
                     EVQDIPAIAKAAHAAGAVVILDNTWATPLFFKPFAGGADVSIQAATKYIGGHADIMLG
                     SVTMNERTNLAV"
BASE COUNT          516 a          876 c          838 g          456 t
ORIGIN      
        1 cattccgctc attaggttgc cgcttcgagc agccataaag ctgaaggccc cccgtgcgtc
       61 cccgcaacgg catcatcgaa gcgatcggca acaccccgct gatcaagctc aagcgcgcgt
      121 cagaagagac tggctgcacc atcctcggca aggccgagtt catgaatccc ggccaatcgg
      181 tgaaggatcg ggccgcgctc ttcatcattc gcgacgcgct gaattcggga cgccttcagc
      241 cgggcggcac cgtggtcgag ggcaccgccg ggaataccgg catcgggctc gcgctggtcg
      301 gcaatgcgct gggcctaaag acggtgatcg tcatccccga gacgcagtcg gacgaaaaga
      361 aggacatgct gcgactggcc ggcgcccggc tcatcgaggt gcccgccgtt ccctacaagg
      421 accccaacaa ctacgtaaaa tactcgggcc gtctcgccga ggagcttgcc tcgagcgagc
      481 cgaacggcgc gatctgggcc aatcagttcg acaacgtcgc caaccgccag gggcatatcg
      541 agacgacggc gcaggagatc tggaaggagc tcggcggcaa ggtcgacggg ttcatctgcg
      601 cggtcggcac cggcggcacg cttgccggcg tctccatggg tctcaaggag cacaacaaag
      661 acgtggtgat cgggcttgcc gacccgatgg gtgccgcgct gtacaattat tacaagcacg
      721 gcgtgctcaa gtcggagggc agctccatca ccgaggggat cggccaggga cgaatcaccg
      781 ccaatctgga gggagccaag atcgacgagg cctaccagat cccggacgcc gaagcgctac
      841 ccgtcatttt cgaccttttg agcgaggaag ggctctgtct cggcggctcc agcgggatca
      901 atgtcgccgg cgcgatccgc cttgcccgcg atttgggccc aggccatacc atcgtcacgg
      961 tgctttgcga ttacggtacc cgctaccaaa gccgcctgtt caaccccaaa ttcctgcgtg
     1021 agaaggggtt gccggtcccg gcatggctcg accaggggca ggctgccgcg cctcgcgtat
     1081 tcgtctaatt cgagaattgg agactagcct tgctgcagac gaagacttgg ctgactgaga
     1141 ccgacgagct tgcccgcgag ctcgacgcgc ccgacctcgt catcatcgat gcctcctggc
     1201 acatgccggc cgagaaccgg aacgcgcatg aggaatatct ggccgagcat attccgggcg
     1261 cgatcttctt cgatatcgac gagatcgccg ataccaaaag cgggctcccg catatgctgc
     1321 cgccgcccga gaaattctcc tcgcgcatgc gcagcatggg aatcggcgac ggctcgcgca
     1381 tcgtggtcta cgattcgagc ggactgttca gcgccgcgcg cgtgtggtgg accttccgcg
     1441 tcatgggcgt cgacgatgtg agcgtgctga acggcgggtt gccgaaatgg aagcgcgagg
     1501 gcctgccgct cgagagcggg ccggcccgaa gccgtaccgc gcgccacttc acggcgcggc
     1561 gcaatctcga tctcgtgcgc gacgtcggcg acatcaaggc gctgctcaag gacagatcgg
     1621 ccgagattgt cgacgcacgc gcggccgatc ggtttgccgg caaggcgccc gagccacgcc
     1681 ccgggcttcg ctcgggccgc atccccggct cgcataatct tcctttcgcc aagcttctca
     1741 ataaggacgg cacactcaag acggcgcctg aaatcgaaag attgttcgag gaagccggtg
     1801 tcgatctctc caagccggtg gtggcgagct gcggctccgg catcacggcg agcgtgcttg
     1861 cgctcggtct tgccgagatc gggcatcgcc gggcggctgt gtatgacggc tcatggtcgg
     1921 agtggggcgc cgaccagaac cttcccatcg agaccggctg aggatcccgc gggtcccctc
     1981 gcccgaccga ggctagatga gcaaacggag caggacacaa aaggatctga ctcggctggt
     2041 gcatgccggg cgcgaacccg cgcgccatca cggcttcgtc aatacgccga tctatcgcgg
     2101 ctctaccatc ctctttccca ccgtcgaggc gctcgaagcc aacgctcagg acttcaccta
     2161 tggaaggctt ggcacgccca cggtcagggc gctcgaagag gcaatcgcgg aactcgaggg
     2221 cgggcagcgc accctgctca ccccatccgg cctttcagcc atcgccactt cgctgctcgc
     2281 gtttcttgcc gccggcgacg aggtgctggt ctcggacagc gtttatcgtc cgacgcggcg
     2341 cttctgcgac ggcgtgctga agcggctcgg cgtgaagacg atctactacg acccgctcat
     2401 tggcgcaggc atcgagaagc tcatcaccaa gaaaacgaag gtcgtcttca ccgaatcgcc
     2461 tggctcgcag accttcgagg tgcaggacat tccggccatc gccaaggccg cgcatgccgc
     2521 gggcgccgtg gtcatcctcg acaacacttg ggcgacgcca ctgtttttca aaccgttcgc
     2581 cggtggcgcg gatgtctcaa tccaggcggc aacaaaatat atcggtggcc acgccgatat
     2641 catgctcggc tcggtcacca tgaacgagcg aaccaacctt gcggtc
//
LOCUS       DATNAE010000092         2380 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_698608, whole genome shotgun sequence.
ACCESSION   DATNAE010000092 DATNAE010000000
VERSION     DATNAE010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698608"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..659)
                     /gene="leuD"
                     /locus_tag="VN518_01980"
     CDS             complement(54..659)
                     /gene="leuD"
                     /locus_tag="VN518_01980"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016918570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HXE03372.1"
                     /translation="MQKFTTLTGIAAYLPMINVDTDMIIPKQYLKTIKRTGLGKGLFA
                     ELRYDEAGKPRADFVLHKPPYHQAKILITGENFGCGSSREHAPWALLDFGIRCIIAPS
                     FADIFYNNCFKNGILPIALPQEEIDKLIDDSARGANATISIDLASQEIRGPDGGKIHF
                     DIDPHRKRCLMEGLDDIDLTMERAEEIDAFERAARLERPWA"
     gene            807..1205
                     /locus_tag="VN518_01985"
     CDS             807..1205
                     /locus_tag="VN518_01985"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HXE03373.1"
                     /translation="MAGTVIYEAHPAMFRAHPFWFILCVLLIAAFGIGILVLLYWYIK
                     TRATALTVTDNELTYERGILSKDRTSVSLKHVRSVHITQGFVNRILGVGIIQISTAGD
                     EPEFTIADMPDPYVIQEAIAKAQDMHDNKD"
     gene            1222..1545
                     /locus_tag="VN518_01990"
     CDS             1222..1545
                     /locus_tag="VN518_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03374.1"
                     /translation="MPSQSDRRERNEALRLFIAAAVALLLLLVIGGAGVYFLLGFNGE
                     GDPFSAGIGLKTAALISFGVSFVAILIMAVVSGGDAILGELPFTIAGFLIFFVIFWLM
                     IAWIF"
     gene            complement(1568..2185)
                     /locus_tag="VN518_01995"
     CDS             complement(1568..2185)
                     /locus_tag="VN518_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03375.1"
                     /translation="MRDRRASLGVALIGALGIALCHAGPAAAQDSWSNEGSTATGPSL
                     DDYLKEAGPDAKLVAQGALKLDGKPVNCGKRPTVLTTNFDSWGGAFPGFLILNTKKIT
                     GLSTQVKLYIYSHECGHQFIGADETEADLFAIRRGVKWGWLDAQGMEDICAFISQLKG
                     DAVHPPGPQRCETMRKYYRELLGSASQPAAAPASSPQAQTLGPPQ"
BASE COUNT          392 a          715 c          789 g          484 t
ORIGIN      
        1 cgacggcaag acgctgctcg agcagagcac caagccgatc ccgttcgagt aggtcaggcc
       61 caggggcgtt cgaggcgggc ggcccgctcg aaggcatcga tctcctcggc cctctccatg
      121 gtgaggtcga tatcgtcgag tccctccatc aggcagcgct tgcggtgggg atcgatgtcg
      181 aaatggatct tgccgccgtc gggccctctg atctcctgac tggcaaggtc gatgctgatg
      241 gtggcgttgg cgccgcgcgc ggaatcgtcg atcagcttgt cgatctcttc ctgcgggagg
      301 gcgatgggca gaatgccgtt cttgaaacag ttgttgtaga aaatgtcggc gaagctcggc
      361 gcgatgatgc atctgatgcc gaaatcgagc agcgcccagg gcgcgtgctc gcggctcgag
      421 ccgcagccga aattctcgcc ggtgatgagg atcttcgcct ggtggtaagg cggcttgtgc
      481 agcacgaagt ccgcgcgcgg cttgccggcc tcgtcgtagc gcagctcggc gaacaggccc
      541 ttgcccagcc cggtgcgctt gatggtcttg agatattgct tggggatgat catgtcggtg
      601 tcgacattga tcatgggcag ataggcggcg atcccggtca gcgtggtgaa tttctgcatg
      661 atgtcctcat tcgacgaaag cctatgtagc ggcaagcggc cgagctttcc agtcgtatgg
      721 ccgtcttaaa gcgcgcttct ccgcttttca gggctcaagg caggccctaa ggtccgcggg
      781 gcacagcgtg gaacgaggga gcgcagatgg cgggaacggt gatttacgag gcgcacccgg
      841 cgatgttccg ggcgcatccc ttctggttca tcctctgcgt cctgctgatc gcggcgttcg
      901 gcatcggcat tctcgtcttg ctctattggt acatcaagac gcgcgccacg gcgctcaccg
      961 tcaccgacaa cgagctgacg tatgagcggg gcatcttgag caaggaccgc acgtcggtgt
     1021 cgctcaagca cgtccgctcg gtgcatatca cccaaggctt cgtcaatcgc attctcggcg
     1081 tggggatcat ccagatctcg accgccggag acgagccgga gttcaccatc gccgacatgc
     1141 ccgacccgta tgtgatccag gaggcgatcg ccaaggccca ggacatgcac gacaacaagg
     1201 attgaacgag gacggcggca gatgccaagc caaagcgacc ggcgcgagcg caacgaggcg
     1261 ttgcggctgt tcattgccgc cgcggtggcg ctgctgttgc tgctcgtgat cggcggcgcc
     1321 ggagtctatt tcctgctcgg gttcaacggc gagggtgacc cgttctcggc gggcatcggg
     1381 ctcaagaccg ccgcgctgat ctctttcggc gtgtccttcg tcgccatttt gatcatggcg
     1441 gtggtgtcgg gcggcgacgc cattctcggc gaactgccct tcaccatcgc cgggtttctg
     1501 atcttcttcg tgatcttctg gctgatgatc gcctggatct tttgacccgc gcgatgccgc
     1561 gccgcggcta ttgcggcggg cccagcgtct gggcctgcgg cgaggaggcg ggcgcggcgg
     1621 ccggttgcga tgccgagccg agcagctcgc gataatattt gcgcatggtc tcgcagcgct
     1681 gcgggccggg cggatgcacc gcgtcgccct tcaactgcga gatgaaggcg cagatgtcct
     1741 ccatgccttg cgcatcgagc cagccccatt tcactccgcg gcggatggcg aagagatcgg
     1801 cttccgtctc gtcggcgccg atgaactggt ggccgcattc atggctgtag atatagagct
     1861 tcacctgggt ggagagaccg gtgatcttct tggtgttgag gatcaagaag cccggaaagg
     1921 cgccgcccca actgtcgaaa ttggtggtga gcacggtcgg ccgcttgccg caattgaccg
     1981 gcttgccgtc gagcttgagc gcgccttggg cgacgagctt ggcgtcgggg ccggcctctt
     2041 tcagatagtc gtcgaggctc gggccggtag cggtcgagcc ttcattggac cacgagtcct
     2101 gcgcggctgc ggggcccgcg tgacagagcg cgatgcccaa ggcgccaatc agcgccacac
     2161 ccaaacttgc ccgtcgatcc ctcatgtcga atcgtactct aaggcgcggc caatccgcgc
     2221 gcaattgcag gaatgtctca cctgccatct tctctcgcgg agagctcgct tttcttggca
     2281 aagcattgcc ttagctcgtc gcagcgcgcc tccgggtcgg ggtgcatggg gccatggccg
     2341 gcgatgttga acagcgcgca gacgtcatcg agggcttgtt
//
LOCUS       DATNAE010000093         2139 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3107009, whole genome shotgun sequence.
ACCESSION   DATNAE010000093 DATNAE010000000
VERSION     DATNAE010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3107009"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1058)
                     /locus_tag="VN518_02000"
     CDS             complement(<1..1058)
                     /locus_tag="VN518_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HXE03376.1"
                     /translation="MTDTIKFFLDGREVEAEPGETIWQVASRCGTEIPHLCYSPAPGY
                     RPDGNCRACMVEIEGERVLAASCIRKPSSGMKVKSASERAKQSRKLVFELLVADQPAR
                     ETAHDPASRFWQWADRVGVDDSRFPVRAQPAPDRSHPAMAVNLDACIECTLCVRACRE
                     VQVNDVIGMAGRGHSEKIVFDFDDPMGQSTCVACGECVQACPTGALMPASQVDQNNVF
                     TNKPDRTVDSVCPYCGVGCQLTYQIKDDRIVNVVGRDGPSNQNRLCVKGRFGFDYVHH
                     PHRLTQPMIRKDGVPKHANDEIDPANPWTHFRPATWEEALDRAASGLKRIRDQHGGKA
                     LAGFGSAKGSNEEAYLFQ"
     gene            complement(1074..>2139)
                     /locus_tag="VN518_02005"
     CDS             complement(1074..>2139)
                     /locus_tag="VN518_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HXE03377.1"
                     /translation="VLKALEDSALKGLGGAGFPTGRKWRFVRAEPGPRLMAVNGDEGE
                     PGTFKDRVYLETDPHRFLEGMLIGAWAVEAPEVYLYLRDEYPQIRELLMREMPLIEAA
                     GLLHHVRVHLRRGAGAYICGEESAMLESIEGKRGLPRHKPPYPSQVGLFGRPTLINNV
                     ETLYWVRDIVEKGPEWFAAQGRRGRKGPRSFSVSGHVKNPGVKLAPAGITVRELIDEF
                     CGGMADGHIFKGYLPGGASGGVLPASMADLPLDFGTLEQYGCFIGSAAVVVLSDKDNM
                     RDVALNLLRFFEDESCGQCTPCRVGTEKAVKLMSERNWDKPLIEDLIKVMGDASICGL
                     GQAAMNPVRSVMKHFPEDVP"
BASE COUNT          369 a          701 c          699 g          370 t
ORIGIN      
        1 ttctggaaga gataggcctc ttcgttcgag cccttggccg agccgaagcc ggcaagcgcc
       61 ttgccgccat gctggtcgcg gatgcgcttc agccccgacg cggcgcggtc gagcgcctcc
      121 tcccaggtcg cggggcggaa atgcgtccag ggattggccg ggtcgatctc gtcattcgcg
      181 tgcttgggaa cgccgtcctt gcggatcatc ggctgggtga gccggtgcgg atgatgcaca
      241 taatcgaagc cgaagcgccc cttgacgcag agacgattct ggttcgaagg accgtcgcga
      301 ccgaccacgt tgacgatgcg gtcgtccttg atctgatagg tgagctggca gccgacgccg
      361 caatagggac agacgctgtc gacggtacga tcgggtttgt tggtgaagac gttgttctgg
      421 tccacctggc tcgccggcat cagcgcgccg gtgggacagg cctgcacgca ctcgccgcag
      481 gcgacgcagg tgctttgccc catgggatcg tcgaagtcga agacgatctt ctcgctatgg
      541 ccgcggccgg ccatgcctat gacgtcgttc acctgcactt cgcggcaggc gcggacgcag
      601 agagtgcatt cgatgcaggc gtcgagattg accgccatcg ccgggtggct gcggtcgggg
      661 gcgggctggg cgcgcaccgg aaagcggctg tcatccacgc cgacgcggtc cgcccattgc
      721 cagaagcggg aagccggatc gtgggccgtc tcgcgcgccg gctgatcggc aaccagcagc
      781 tcgaagacca gcttgcgcga ttgcttggcg cgctcggaag cgctcttcac cttcatgccg
      841 gaagagggct tgcggataca ggaggcggcg agcacgcgct cgccttcgat ctcgaccatg
      901 cacgcgcggc aattgccgtc ggggcgatag cccggcgccg ggctgtagca gagatgcgga
      961 atctcggtgc cgcagcggct tgccacctgc cagatggtct cgccgggctc ggcctcaact
     1021 tcccggccgt cgagaaagaa cttaatcgta tcggtcatgc ctgacctctc ccgttacggc
     1081 acatcttcgg gaaaatgctt catcaccgag cggaccggat tcatcgccgc ctggccgaga
     1141 ccacagatcg aggcatcacc cattaccttg atcaggtcct cgatgagcgg cttgtcccag
     1201 ttgcgctctg acatcagctt caccgccttc tcggtcccga cccggcaagg cgtgcactgg
     1261 ccgcaactct catcctcgaa gaagcgcagg aggttgagag cgacgtccct catgttgtcc
     1321 ttgtccgaga ggacgacaac cgccgccgag ccgataaagc agccatattg ctccagcgtg
     1381 ccgaaatcga gcggcagatc ggccatcgag gccggaagca cgccgcccga ggcgccgccc
     1441 ggcagatagc ccttgaaaat gtggccgtct gccatcccgc cgcaaaactc gtcgatgagc
     1501 tcgcgcacgg tgatgccggc gggcgccagc ttgacgccgg gattcttcac atggcccgag
     1561 accgaaaagc tgcgcgggcc cttgcggccg cgccgacctt gcgccgcgaa ccactcgggc
     1621 cccttctcaa cgatgtcgcg cacccagtag agcgtctcga cattgttgat aagggtgggc
     1681 cggccgaaca gtcccacctg tgagggatag ggcggcttgt ggcgcggcag gcctcgcttg
     1741 ccctcgatgc tttccagcat cgccgattcc tcgccgcaga tataggcacc ggcgccgcgc
     1801 cgcagatgca cgcgaacatg gtgcagcagc cctgccgcct cgatgagcgg catctcgcgc
     1861 atgagcagct cgcgaatctg cggatattcg tcgcgcagat agaggtagac ctcgggagct
     1921 tcgacagccc aggcgccgat cagcatgcct tcgagaaagc ggtgcgggtc ggtctcgaga
     1981 tagacgcggt ccttgaaagt gcccggctcg ccctcgtcgc cattgaccgc catgaggcgc
     2041 ggtcccggct cggcgcggac gaagcgccat ttgcggccgg tggggaaccc cgcgccgccg
     2101 agtcccttga gcgcggaatc ctcgagcgcc ttcagcacg
//
LOCUS       DATNAE010000094         3663 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_398861, whole genome shotgun sequence.
ACCESSION   DATNAE010000094 DATNAE010000000
VERSION     DATNAE010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398861"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..308
                     /locus_tag="VN518_02010"
     CDS             129..308
                     /locus_tag="VN518_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010685332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 family protein"
                     /protein_id="HXE03378.1"
                     /translation="MTLLKWALICLLISLVAGAFGYTGLARGAGQLAKILFFIFLIVF
                     IIILILAISAGQLVF"
     gene            complement(305..1501)
                     /locus_tag="VN518_02015"
     CDS             complement(305..1501)
                     /locus_tag="VN518_02015"
                     /inference="COORDINATES: protein motif:HMM:NF015541.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXE03379.1"
                     /translation="MPKIDACLTRAAKAAPALCLGFVLSFALGREAAALYDVKPGDAA
                     GKPGSIIRVWPLEGGGPGGANAFRILYRSTSPSGEPIAVSGAIFIPPGSPPPGGRNVI
                     AWAHPTSGVVEDCAPSLMPDLAGMIWGLAGMMAQGYVVVATDYPGLGVPGMMHPYLIG
                     PSEARAVLDSVRAARDLPDAAASNRFAVWGHSQGGHASLYTGELAASYAPDLKLVGVA
                     AAAPATYLVELFDADKTSSEGKELTAMTVLSWSKLENLPVTTIVEPDAVPAFEKTARA
                     CIESLAQFEAINKDEQPLQNIQFLKADPSETEPWKGIMLQNTPGQAPAGAPVFIAQGT
                     ADTTVRPEITKQFGDHLCSQGVRVDFVELPGVTHTFAAKDSAPAALAWMGERFRGLPA
                     PSSCER"
     gene            1697..2842
                     /locus_tag="VN518_02020"
     CDS             1697..2842
                     /locus_tag="VN518_02020"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXE03380.1"
                     /translation="MTRAAATIDALLNRVGKIATPFTIELPDGEKRTLGEGKPEFEVG
                     LRNERALRALRSLDEGNIAEAYLQGDIDLEGDMMKPFDLRASLDDRHPLVAAWRFIEP
                     LMFGQIYTNKRAISSHYNADPKLFLSFLDPIFPAYSQGVYENDDESLSTALQRKFDWA
                     LEQCRIGKGTRVLEIGPGWGAFAGHALQAGARFTGITNSEVSQSYLRGKLANFGDNFA
                     IELTDFYEYQPKEKFDAIVIMGVIEHLPDYERVLKKFSSLLKPGGRVFLDGSAWSKKY
                     ELSTFMVRHIYPGNHSFLVLDDFLNKLWKTDFEVDVIHNDRWSYYLTFRQWARNLDAS
                     RDYITRTFGDFEFRKFRLYLWGATYEFLHRNLDCYRLILSKPKQSPA"
     gene            3046..>3663
                     /locus_tag="VN518_02025"
     CDS             3046..>3663
                     /locus_tag="VN518_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015933605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HXE03381.1"
                     /translation="MPFAFARADQPVSLTPPQNTMSPAEALDRLMQGNARYVAGETEC
                     QDFSIGRAERAGAQYPIVGVLSCSDSRVSPELLFEQGPGDIFVVRVAGNFVNTDGLAS
                     MEYAVKILGVPLLMVLGHTNCGAISAAVKVVTEHAELPGHLPELVASIEPAVIAAHAK
                     HSSDLVAATIEENVRLNVKRLIDDTPILADALAAKKIAISGGVYDI"
BASE COUNT          639 a         1192 c         1154 g          678 t
ORIGIN      
        1 cgggaacacg gtgatcaaga tcagctattg agccaaagcg gcaggtcgcc cccttgctcc
       61 ttgtctgatc ttctgtagca tcgctgcgag ggaagggcgc tcgaaccgcc cgcttgaagg
      121 agggggatat gacgctgctg aaatgggcgc tgatctgcct gctgatctcg ctggtcgccg
      181 gcgcatttgg ctataccggt ctcgcccgcg gcgcgggcca gctcgccaag attctgttct
      241 tcatcttcct catcgtcttc atcatcattc tgatcctggc gatctcagcc ggccagctcg
      301 tgttctagcg ttcgcaactc gacggcgccg gcaggcccct gaagcgctcg cccatccagg
      361 ccagcgccgc cggcgcgctg tccttggcgg cgaaggtatg cgttaccccc ggcagctcga
      421 cgaagtcgac gcgcacgcct tggctgcaga ggtggtcgcc gaattgcttg gtgatctcgg
      481 gcctgaccgt cgtatcggcg gtgccctggg cgatgaacac cggcgctccg gcaggcgctt
      541 gacccggcgt attctgcagc atgatcccct tccaaggttc ggtctcggac gggtcggcct
      601 tgaggaactg gatgttctgc aagggctgct cgtccttgtt gatggcctcg aattgggcga
      661 gcgattcgat gcaggcgcgc gccgtctttt cgaaggccgg cacggcgtcg ggttcgacga
      721 tcgtcgtcac cgggaggttc tcgagtttcg accaggagag caccgtcatc gccgtcagct
      781 ccttgccctc cgagctcgtc ttgtcggcgt cgaacagctc gacgagatag gtggcgggcg
      841 ccgccgccgc gacgccgaca agcttcaggt cgggcgcgta gctcgcggcc agctcgccgg
      901 tatagagcga tgcgtggccg ccttggctgt gtccccacac cgcgaaccgg ttggaggcgg
      961 cggcgtcggg cagatcgcgc gccgcgcgca ccgaatcgag caccgcgcgg gcttcgctcg
     1021 ggccgatgag ataagggtgc atcatccccg gcacgccgag gccgggatag tcggtggcga
     1081 cgacgacata gccttgcgcc atcatgccgg ccaatcccca gatcatgccg gcgaggtcgg
     1141 gcatcaacga cggcgcgcag tcctcaacca cgccgctggt cgggtgcgcc caggcaatca
     1201 cgttgcggcc gcccggcgga ggcgaccccg gcggaatgaa gatggcgccg gacacggcaa
     1261 tcggctcgcc ggaggggctg gtcgaacgat agaggatgcg gaaggcattg gcgccgccgg
     1321 ggcctccgcc ttcgagcggc cacaccctga tgatcgaacc cggcttgccc gcggcgtcgc
     1381 cgggcttcac gtcgtaaagc gcggctgcct cccgcccaag ggcgaaactc aggacaaaac
     1441 caaggcaaag ggcaggcgcg gccttggccg cgcgcgtgag gcaggcgtcg atctttggca
     1501 tgcaaatccc ccgggctgag cgctggtgga ccggatatag cctcagacat tggcacttcc
     1561 gcaacagtcg ccagcatggc cggctcagct caccatttca tttcactgtc atccggcgtt
     1621 aaaatcggcg ccgaacgctt atcggcccgt ggcgctggcc cgccgcgggc gcttggccaa
     1681 gccgggggag tgaagcatga cgagagctgc cgcgacgatc gatgccttgc tcaaccgcgt
     1741 gggcaagatc gcgacgccct ttaccatcga gttgcccgac ggcgagaaac ggacactcgg
     1801 cgagggcaag cccgagttcg aagtgggctt gcgcaacgag cgtgcgttaa gggcgctgcg
     1861 ctccctcgac gagggaaata tcgccgaggc ctatctgcaa ggcgacatcg acctcgaagg
     1921 cgacatgatg aagcccttcg atcttcgcgc ctcgctcgac gaccggcacc cgctggtcgc
     1981 ggcctggcgc ttcatcgagc cgctcatgtt cggacagatc tataccaaca agcgggcgat
     2041 ctcctcccac tacaacgccg acccgaagct gttcttgagc ttcctcgatc cgatctttcc
     2101 cgcctatagc cagggcgttt acgagaacga cgacgagtcg ctgtcgacgg cactccagcg
     2161 gaagttcgac tgggcgctcg agcaatgtcg catcggcaag ggcacgcgcg tgctggagat
     2221 cggtccgggc tggggcgcct tcgccggaca tgcgctgcaa gccggcgcac gcttcaccgg
     2281 catcaccaac tcggaggttt cccagagcta tctccgcggc aagctcgcca atttcggcga
     2341 caatttcgct atcgagctga ccgacttcta cgaatatcag ccgaaggaga agttcgacgc
     2401 catcgtcatc atgggggtga tcgagcacct ccccgactac gagcgcgtgc tgaagaagtt
     2461 ctcctcgctg ctcaagcccg gcggccgcgt cttcctcgat ggcagcgcct ggagcaagaa
     2521 atacgagcta tcgaccttca tggtgcgcca catctatccc ggcaaccatt ccttcctggt
     2581 gctcgacgat ttccttaaca agctgtggaa gacggacttc gaggtggatg tcatccacaa
     2641 cgatcgctgg agctattact tgaccttccg gcaatgggcg cggaacctcg atgccagccg
     2701 cgactacatc acgcggacct tcggcgactt cgaattccgc aaattccggc tctatctctg
     2761 gggggcgacc tacgagttcc tccaccgcaa tctcgattgc taccggctga tcctctccaa
     2821 gccgaagcaa agccccgcct aggactggcg gcgacggctt acggccgcgt ttgcgactcc
     2881 ctcaccaaag ctccgatccg ggcttgaccc ccgcttggct tgcgggagag gtaaggcacc
     2941 ctatggagga gaatcacgat gtgcgagctt tgccagcctt cgcttgcttc gatatcacgg
     3001 cgccatctca tggcgggggg agccgccttt ctcgccgcta gcgctttgcc tttcgctttc
     3061 gcccgcgccg atcagccggt ctccctgacg ccgccacaga atacgatgtc gccggccgag
     3121 gcgctcgacc ggctgatgca gggcaatgcc cgctatgtgg cgggcgagac cgagtgccag
     3181 gacttctcca tcggccgcgc cgagcgcgcg ggcgcgcaat accccatcgt cggggtgctg
     3241 agctgctcgg acagccgagt gtcgcccgag cttctcttcg agcagggacc gggcgacatt
     3301 tttgtcgtgc gcgttgccgg caatttcgtc aatacggatg ggctggcgag catggaatat
     3361 gcggtgaaga ttttgggcgt gccgcttctt atggtcctcg gacacaccaa ttgcggcgcc
     3421 atttccgcag cggtcaaggt cgtgacggag cacgcggagc tgccgggcca tctgccggag
     3481 ctggtcgcgt ccatcgagcc cgcggtcatt gccgctcatg cgaaacattc gagcgatctc
     3541 gtcgccgcca ccatcgagga gaacgtgcgc ctcaatgtca aacggctgat cgacgacacg
     3601 ccaatcctcg ccgatgcgct tgcggcgaag aagattgcga tctcgggcgg cgtctacgat
     3661 att
//
LOCUS       DATNAE010000095         3194 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2811089, whole genome shotgun sequence.
ACCESSION   DATNAE010000095 DATNAE010000000
VERSION     DATNAE010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2811089"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="VN518_02030"
     CDS             <1..413
                     /locus_tag="VN518_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007633778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HXE03382.1"
                     /translation="ATLRVVREVGYASAFCFKYSPRPGTPAAGLAAQVPEELKTERLA
                     RLLELVTAQQRQFARDCVGMTLDVLLEKPGRMAGQLGGRSPYLQAVHLEAPQSLIGTI
                     QPVLITAAGNNSISGKIVTDSEHKSASFVEVLAG"
     gene            516..1547
                     /locus_tag="VN518_02035"
     CDS             516..1547
                     /locus_tag="VN518_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HXE03383.1"
                     /translation="MSPSADNALSTQLELAFDDNRLAAQLYGDFDQNLALIEQRLGVS
                     ATPRGNHVLLKGGASGIDQARRVLESLYAMLEEGRAIDISNVDSIIRMIESEDSQLSL
                     PTLEKKGKVRMAQIATRKATIVARTPAQDAYMRAMERAELVFGVGPAGTGKTYLAVAH
                     AATLLERGDINRIILSRPAVEAGERLGFLPGDMKEKVDPYLRPLYDALYDMMRPEHVE
                     RCITSGIIEVAPLAFMRGRTLANAVVILDEAQNTTSMQMKMFLTRLGENSKMIVTGDP
                     TQVDLPRGEKSGLIEAVALLEGVEGVHISRFNDKDVVRHALVGRIVRAYEADTERRLA
                     AQRREDRQP"
     gene            1610..2026
                     /gene="ybeY"
                     /locus_tag="VN518_02040"
     CDS             1610..2026
                     /gene="ybeY"
                     /locus_tag="VN518_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004120814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HXE03384.1"
                     /translation="METLADRGVFEALAQSGARVTGVCELSIVLTDDAEQQQLNREWR
                     GIDKPTNVLSFPQIAPFGPVTGILGDIVLARETLEREAAELEKSFADHFTHLVVHGFL
                     HILGYDHIEEGEALEMEGLETKILATLGIADPYSDT"
     gene            2001..3098
                     /locus_tag="VN518_02045"
     CDS             2001..3098
                     /locus_tag="VN518_02045"
                     /inference="COORDINATES: protein motif:HMM:NF015436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HXE03385.1"
                     /translation="MPILIPTLERRPWRLMNMTDDRTVTAEPPSSPAPAAPRGHPLWH
                     RFKALLALRSSSVRDDLEVLLESEASAETSDFSEAERKILQNVLKLEDLSVDDVMVPR
                     ADIVAIDVNETLGTLLARFLEAGHSRIPVYDDDLDNITGFVHVKDALRRIVEVPPLDD
                     STEEARVKVVGTGLRSRIGKLEVVREVLFVPPTMPVGDLLQQMQLKRVHMAIVIDEYG
                     GTDGVVTIEDLLEAVVGEIEDEHDEEDRPLIRKLNANNFIASARAELGEVREAVGAAF
                     DPGELADEVDTIGGLVFDLAGHVPPPGEVVTGVNGVSGFEFEVLQSDGRQIQKLKIRR
                     VKPAARPRPKAKPAAAPPEAPASTNEAKAAE"
BASE COUNT          576 a         1097 c          985 g          536 t
ORIGIN      
        1 aagcgacgct tcgggtggtg cgcgaggtcg gctacgcctc ggccttctgc ttcaagtatt
       61 cgccccgccc gggcacgccc gccgccggcc tcgctgcgca ggtgcccgag gagctgaaga
      121 ccgagcggct ggcgcgcctg ctcgagctcg tcacggccca gcagcgccag ttcgcccgcg
      181 attgcgtcgg catgacgctt gatgtgctcc tcgaaaagcc cggccgcatg gctgggcaat
      241 tgggcggccg ctccccatat cttcaggcgg ttcacctcga ggccccgcaa agcctgatcg
      301 gcaccatcca gccggttctc atcaccgcgg cgggaaacaa ttcaatttcg ggcaagattg
      361 tcacagactc ggaacacaaa tctgctagct ttgttgaggt attggccggc tgagcatcga
      421 tcggcgatgt ccgagctgcg gccatctgga gcgcgaacgg cacaagtgcg gaacagtgtt
      481 gcgtcgcgtg ggagcatccc cgcttgagca ggcatttgtc cccttctgcc gacaacgccc
      541 tttccaccca gcttgaactt gccttcgacg acaaccggct cgctgcccag ctctatggcg
      601 atttcgacca gaatctcgcc ctgatcgagc agcgcctcgg cgtatcggcg acgccgcgcg
      661 gcaaccacgt gctgctgaag ggcggcgcga gcggcatcga ccaggcccgc cgggtgttgg
      721 aatcgctcta tgcgatgctc gaggaaggcc gcgccatcga catctccaat gtcgattcga
      781 tcatccgcat gatcgagagc gaggattccc agctgagcct gcccaccctt gaaaagaagg
      841 gcaaggtcag gatggcgcag atcgcgacgc gcaaggcgac gatcgtcgcc cgcaccccgg
      901 cgcaggatgc ctatatgcgc gccatggagc gcgccgaact cgtcttcggc gtcggccccg
      961 ccggcaccgg caagacttat ctcgccgtcg cccacgcggc gacgctcctc gagcgtggcg
     1021 acatcaaccg catcatcctc tcgcggcccg cggtcgaggc cggcgagcgc cttggcttcc
     1081 tccccggcga catgaaggaa aaggtcgatc cctacctcag gcccctgtat gacgccctct
     1141 acgacatgat gcgccccgag cacgtcgagc gctgcatcac ttccggcatc atcgaggtgg
     1201 ccccgctcgc cttcatgcgc ggccgcaccc ttgccaacgc cgtggtgatc ctcgatgagg
     1261 cgcagaacac cacctcgatg cagatgaaga tgtttctgac ccgcctcggc gagaactcga
     1321 aaatgatcgt caccggcgat ccgacccagg tcgacctgcc gcgcggcgaa aaatccgggt
     1381 tgatcgaagc cgtggcgctg ctcgaaggcg tcgagggcgt ccacatttcg cgcttcaacg
     1441 acaaggacgt cgtgcgtcac gccctcgtcg gccgcatcgt ccgcgcctat gaggccgaca
     1501 ccgagcgccg tctcgccgcg cagcggcgcg aggatcggca gccctgagca cgaagcgccc
     1561 gcccctcacc attctgctcg ccatcaattc cgaaggctgg cccgagggga tggagacgct
     1621 cgccgaccgc ggcgtgttcg aggcgctggc gcagtcgggc gcccgcgtca ccggcgtctg
     1681 cgagctctcg atcgttctga ccgacgacgc cgaacagcag cagctcaacc gcgagtggcg
     1741 cggcatcgac aagccgacca acgtcttgag ttttccccag atcgccccgt ttggcccggt
     1801 aaccggcatt ctcggcgaca tcgtcctcgc ccgggaaacc ctcgagcgcg aggcggcgga
     1861 gcttgaaaag tccttcgccg atcacttcac ccatctggtg gtgcatggat tcctgcacat
     1921 cctggggtat gatcacatcg aggagggcga ggcccttgaa atggaagggc tcgagacgaa
     1981 gattctcgca accctcggca ttgccgatcc ttattccgac acttgagcgg cgcccctggc
     2041 gcctgatgaa catgaccgac gaccgaactg ttaccgccga gcctccttct agccccgccc
     2101 ccgccgcgcc gcgggggcat ccgctttggc accggttcaa ggcgctgctg gcgctgcgca
     2161 gcagctcggt ccgcgatgac ctcgaagtgc tgctcgaaag cgaggccagc gccgaaacct
     2221 cggatttctc cgaggccgag cgcaagatcc tgcagaacgt tttgaagctc gaagatctct
     2281 cggtcgacga tgtcatggtg ccgcgcgccg atatcgttgc gatcgacgtc aacgagacgc
     2341 tcggcacgct tctcgcccgc ttcctcgagg ccggccactc gcgcattccg gtctacgacg
     2401 acgatctcga caacatcacc ggcttcgtcc acgtcaagga cgcgctgcgc cgcatcgtcg
     2461 aggtgccccc gctcgacgat tcgaccgagg aggcgcgcgt caaggtggtg gggacgggcc
     2521 tccgcagccg catcggcaag ctcgaagtgg tgcgcgaggt gctgttcgtg ccccccacca
     2581 tgccggttgg cgatctcctg cagcagatgc agctgaagcg cgtccacatg gcgatcgtca
     2641 tcgatgagta tggcggcacc gatggcgtcg tcaccatcga ggacttgctc gaggcggtgg
     2701 tcggcgagat cgaggacgag cacgacgagg aagatcgtcc gctgatccgc aaactcaacg
     2761 ccaacaattt catcgcctcg gcccgcgccg agctcggcga ggtgcgcgag gcggtgggcg
     2821 ccgccttcga tcccggcgaa ctggccgacg aggtggatac catcggcggc ctcgtcttcg
     2881 accttgccgg ccacgtgccg ccacccggcg aggtggtgac cggcgtcaac ggcgtctcgg
     2941 gcttcgagtt cgaagtgctg caatccgatg gccggcagat ccaaaagctc aagatcaggc
     3001 gcgtaaaacc cgccgcccgg ccacgaccca aggcaaagcc cgccgctgct ccgccagaag
     3061 caccggcgtc gaccaatgag gcaaaggcag ctgagtaggc cgccgcgaat cgcgcttttg
     3121 caggactcca gcttgccggc gagtcaagcg aagattatct tgatgcaatg accgaacttg
     3181 ctgatctggc ccac
//
LOCUS       DATNAE010000096         3662 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2508106, whole genome shotgun sequence.
ACCESSION   DATNAE010000096 DATNAE010000000
VERSION     DATNAE010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2508106"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /gene="eno"
                     /locus_tag="VN518_02050"
     CDS             <1..383
                     /gene="eno"
                     /locus_tag="VN518_02050"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007067356.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HXE03386.1"
                     /translation="ALGDRVQLVGDDLFVTNVTRLKQGIDRHIANAILVKVNQIGTLS
                     ETLDAVRMAQDAGYAAVMSHRSGETEDATIADLAVATNCGQIKTGSLARSDRVAKYNQ
                     LIRIEEALGASARYAGRSVLKTEK"
     gene            432..761
                     /locus_tag="VN518_02055"
     CDS             432..761
                     /locus_tag="VN518_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006934547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HXE03387.1"
                     /translation="MRFTAFIFPLLACGAAGYFGYHLETGDHGWKARADLERRKDVLA
                     GELAGLREVKGRLERDVALLRPESLDPDMLDERARAILNLAHQDDLIMMKRRQPVPDA
                     AGSSPKR"
     gene            838..1884
                     /gene="pdhA"
                     /locus_tag="VN518_02060"
     CDS             838..1884
                     /gene="pdhA"
                     /locus_tag="VN518_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HXE03388.1"
                     /translation="MTTSPSLSPQAANFWLGDTTGAPGGRSTLSPGQELAAYRLMLLI
                     RRFEEKAGQLYGMGYIGGFCHLYIGQEAVVAGMQMAIADGDQVITAYRDHGHMLAAGM
                     EAKRVMAELTGRRHGYAKGKGGSMHMFSVEKNFYGGHGIVGAPAPLGTGLAFANKYRG
                     NDRVCLTFFGEGAANQGQVYESFNMASLWKLPVIYVIENNHYAMGTHVERGSALAEEL
                     YHRGSVFGIPGEQVDGMDVAAVKAAGDRAVASARRGEGPKILEMLTYRYRGHSMSDPA
                     KYRTREEVQEIREHRDPIDLLRDRILKEGRASEAELKEIDREVRARVNEAAEFAQNDP
                     EPDPSELFTDVTAN"
     gene            1902..3425
                     /locus_tag="VN518_02065"
     CDS             1902..3425
                     /locus_tag="VN518_02065"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012091971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HXE03389.1"
                     /translation="MPSEILMPALSPTMEEGKLVKWLVREGDRVRAGDVIAEIETDKA
                     TMEVEAAEDGKLGTILVAEGSEHVPVNQPIALLLTDGEDAAEASSFETRPAAAPQDEA
                     PASRTASAPQPEEGASAPVSKGEGTQAISPSPQPSPQRGEGAARELGESLPDVERAPA
                     GPQLAPAEPRPDAEIAPGTEMVRMTVREALRDAMAEEMRRDPAVFVMGEEVGEYQGAY
                     KVTQGLLDEFGPTRVVDTPITEYAFAGVGVGAAFAGLKPIVEFMTWNFAMQAIDHIIN
                     SAAKTLYMSGGKMHCPIVFRGPNGAAARVAAQHSQNYAAWYAHVPGLKVIAPYTAADA
                     KGLLKAAIRDSSPVVFLENEILYGQNFEVPKLDDFVIPIGKARIARAGKDVTVVSFSR
                     GMAYALDAAKHLAEEGIEAEVIDLRTLRPLDIETVLASVRKTNRIVTVEEGWPVCSIA
                     SEIAARVTAEAFDYLDAPPLAVTGVDVPMPYAANLEKLALPSVEHVIEAVKSVCYRS"
     gene            3431..>3662
                     /locus_tag="VN518_02070"
     CDS             3431..>3662
                     /locus_tag="VN518_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HXE03390.1"
                     /translation="MADTSMDWKTGGCHCGAVRFEVLAPEEIEAKECNCSICRKAGYL
                     HLIVDKERFRLVRGQDKLSTYSFNTGTARHHFC"
BASE COUNT          670 a         1213 c         1198 g          581 t
ORIGIN      
        1 aagcgctggg cgaccgtgtc caactcgtcg gcgacgattt gttcgtcacc aacgtcacgc
       61 ggctgaagca gggcatcgac cggcatatcg ccaatgccat cctggtcaag gtcaaccaga
      121 tcggcaccct gagcgaaacg ctcgacgccg tgcgcatggc gcaagacgcc ggctatgcgg
      181 cggtgatgtc gcatcgctcg ggcgagaccg aggacgccac catcgccgat ctcgccgtcg
      241 ccaccaattg cgggcagatc aagaccggct cgctcgcccg ctccgaccgc gtggcgaaat
      301 ataaccagct gatccgcatc gaggaggcgc tgggcgcatc ggcccgctat gccgggcgct
      361 ccgtattgaa aacggaaaag taacctccgc ttaacccgca gcaggcgatt ctatccggct
      421 tcgggggaaa catgcgcttc acggctttca tcttcccgct gctcgcctgc ggtgctgccg
      481 gctatttcgg ctatcacctg gagaccggcg accatggctg gaaggcgcgc gccgacctcg
      541 agcggcgcaa ggatgtgctc gccggcgagc ttgccggcct gcgcgaggtg aagggccggc
      601 tcgagcggga cgtggcgctg ctcaggcccg agagcctcga ccccgacatg ctggacgagc
      661 gcgcccgcgc catcctgaac ctcgcgcatc aggacgacct catcatgatg aagcgccgcc
      721 agcccgtgcc cgatgcggcg ggatcatcgc cgaagcgcta gacgcccaag ctttcgggga
      781 acttccaact cgtgtaagcc ttgtctttgg gtgaagccca agaaggccac gagtcttatg
      841 accacaagcc caagtttatc gccgcaggcc gcaaatttct ggctcggcga caccacgggc
      901 gcccccggtg gacgctcgac actgagcccg gggcaggaac tcgccgccta ccgcctgatg
      961 ctgctcatcc gcaggttcga ggagaaggcg gggcagcttt acggcatggg ctatatcggc
     1021 ggcttctgcc atctctatat cggccaggaa gcggtcgtcg ccggcatgca gatggcgatc
     1081 gccgacggcg accaggtcat caccgcctat cgcgatcacg gccatatgct cgccgccggg
     1141 atggaggcga agcgggtgat ggccgagctg accggccggc gtcacggcta tgccaagggc
     1201 aagggcggct cgatgcacat gttctcggtc gagaagaatt tctacggcgg ccacggcatc
     1261 gtcggcgcac cggcgccgct cggcaccggt ctcgccttcg ccaacaaata tcgcggcaac
     1321 gatcgcgtct gcctgacgtt cttcggcgag ggcgcggcca atcagggcca agtctatgag
     1381 agtttcaaca tggcgtcgtt gtggaagctg ccggtgatct acgtgatcga gaacaatcac
     1441 tatgccatgg gcacccatgt tgagcgcggc agcgccctcg ccgaagagct ttatcatcgc
     1501 ggctcggtgt tcggcattcc cggcgagcag gtcgacggca tggatgtcgc ggcggtgaag
     1561 gcggccggcg accgcgccgt ggccagcgcg cggcgaggcg aagggccgaa gatcctcgaa
     1621 atgctcacct accgctatcg cggccactcg atgtcggacc ccgccaaata ccgcacgcgc
     1681 gaggaggtgc aggagatccg cgagcatcgc gacccgatcg acctcttgcg cgaccggatt
     1741 ctgaaggagg gccgcgccag cgaggccgag ctgaaggaga tcgaccgcga ggtccgcgcc
     1801 cgcgtgaacg aggcggcgga gttcgcacaa aacgatcccg agcccgatcc ttccgagctt
     1861 ttcaccgacg taacggcgaa ctgagccttc tcccgaaact gatgccaagc gaaattctca
     1921 tgccggcgct ctctcccacc atggaggagg gaaagctcgt caaatggctg gtgcgggagg
     1981 gcgatcgcgt gcgcgccggc gacgtcatcg ccgagatcga gaccgacaag gccaccatgg
     2041 aggtggaagc cgccgaggac ggcaagctcg gtacgattct ggtggccgag ggcagcgagc
     2101 atgtgccggt gaaccagccc atcgcgctgc ttctgacaga cggcgaggat gcggcagagg
     2161 cctcatcctt cgagacgcgg ccggcggccg ctcctcagga tgaggctccg gcgtcgcgca
     2221 ccgcctcggc tcctcagcct gaggagggcg cttcagcacc cgtctcgaag ggtgagggga
     2281 ctcaagccat ttccccctcg ccccaaccct ctccgcagcg gggagaggga gcggcccgcg
     2341 aactcggcga gagcctgccc gatgtcgagc gggcgccggc gggtccgcaa cttgcgccgg
     2401 cggaaccgcg gcctgacgcc gagatcgcgc ccggcaccga aatggtgcgc atgaccgtgc
     2461 gcgaggcgct gcgcgacgcc atggccgaag agatgcgccg cgatccggcg gtgttcgtca
     2521 tgggcgagga ggtcggcgag tatcagggcg cctataaagt cacccagggc ctgctcgacg
     2581 agttcggacc gacccgcgtg gtcgatacgc ccatcaccga atatgccttc gccggggtgg
     2641 gcgtcggcgc ggccttcgcc ggcctcaagc cgatcgtcga gttcatgacc tggaacttcg
     2701 ccatgcaggc gatcgaccac atcatcaatt ccgccgccaa gacgctctac atgtccggcg
     2761 gcaagatgca ttgcccgatt gtgttcagag ggccgaacgg cgccgcggcc agggtggccg
     2821 cccagcacag ccagaattac gccgcctggt acgcgcatgt gccggggctg aaggtgatcg
     2881 cgccttacac cgccgccgac gccaagggcc tgctcaaggc ggcgatccgc gattccagcc
     2941 ccgtcgtgtt cctcgagaac gagatcctct acggtcaaaa cttcgaggtg ccgaagctcg
     3001 acgactttgt catcccgatc ggcaaggcgc gcatcgcccg cgcgggaaaa gacgtcaccg
     3061 tcgtctcctt ctcgcgcggc atggcctatg cgctcgacgc cgccaagcat ctcgccgagg
     3121 aggggatcga ggcggaagtg atcgacctac gcaccctgcg cccgctcgac atcgagaccg
     3181 tgctcgcctc ggtgcgaaag acgaaccgca tcgtcaccgt ggaggagggc tggccggtct
     3241 gctcgatcgc ctcggagatc gccgcgcgcg tcacggcaga agccttcgac tatctcgatg
     3301 cgccgccgct cgccgtcacc ggcgtcgacg tgccgatgcc ttacgccgcc aatctcgaga
     3361 agctggctct gccgtcggtc gagcacgtga tcgaagcggt gaaatcggtc tgctaccgga
     3421 gttgatgccg atggccgata cctccatgga ctggaagacg ggcgggtgcc attgcggcgc
     3481 ggtgcgcttc gaggtgctgg cgcccgagga gatcgaggcc aaggagtgca actgctcgat
     3541 ttgccgcaag gccggctatc tgcacctcat cgtcgacaag gagcgcttcc gcctcgtgcg
     3601 cggccaggac aagctcagca cctacagctt caataccggc accgcgcggc atcatttttg
     3661 ct
//
LOCUS       DATNAE010000097         2599 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_1905103, whole genome shotgun sequence.
ACCESSION   DATNAE010000097 DATNAE010000000
VERSION     DATNAE010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1905103"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..842
                     /locus_tag="VN518_02075"
     CDS             <1..842
                     /locus_tag="VN518_02075"
                     /inference="COORDINATES: protein motif:HMM:NF028178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HXE03391.1"
                     /translation="HDPHIDAGFISTEVLAGAKTQSAGNDLAERLQVVARFCPQEVHV
                     LARADIKSLADLAGKKVNIGPAGGSSAVTATILFRAFNISIEPLALDGHAAIEQLKQG
                     TIAAAVIVGAKPTPLISEISASDGLHLLPIEFGAGLEEAYLPTGFDRDDYPNLIAMET
                     EIPSVATGLVLLAAASKDDDGHTERLAGFVGTMFSRFGELKKDGRHPKWREINLAASL
                     PGWTRNPAAEAWLTEQRKGSARRDPQVRIEAGVVPSHPAVGQDQREALFKQYIEWQRA
                     KGH"
     gene            929..2269
                     /locus_tag="VN518_02080"
     CDS             929..2269
                     /locus_tag="VN518_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03392.1"
                     /translation="MIGSTTPCQAEAGFRPLRAQLAHSHAALARPAAISGEVHQRDYP
                     QIDSQPHPRREAFAMTLPRSLAARLRAFAPDDKELGLFLDDLALAVRTETGSGYDMSD
                     SARFEAGLPLEPQVASPASQAHMQALVHDFRRRQRQASMIVAGCVATSFVLAVVGIAA
                     LASFVKPAPADAEPMIKSSNADVLQQDAEPAKLILAKAAPFTDRLPVEDEDTASLVPD
                     LGAPPASAVNAAPQLIMVQAGRPLELAPLLSQRQARYVLLRGLPNEATLSAGQRNPSG
                     AWLVKDKDVDRLTVTMQSTASGDYPIEVYALGASSAPQARQRLVFRVAAGPTAGVATG
                     ASGVLFNMALLGMAPEQPAATKASPLMTRAMRLLGEGDIAGARLLFMHLAEQGESEAA
                     YELARTFDGEVLSELGATGVGADRTRAVGWYERASETGNAKAAERLKILASLSD"
     gene            complement(2339..>2599)
                     /locus_tag="VN518_02085"
     CDS             complement(2339..>2599)
                     /locus_tag="VN518_02085"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007636902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HXE03393.1"
                     /translation="RRMARELFQDGEFVEVYVETPIEVCEARDPKGLYRKARAGLISH
                     FTGIDSDYEVPEQPELAINTSNTTADALADLLLAELRRRHVV"
BASE COUNT          454 a          875 c          843 g          427 t
ORIGIN      
        1 tgcatgaccc gcacatcgat gcgggtttta tttcgaccga ggtgctcgca ggcgccaaga
       61 cgcaaagcgc cggcaacgac ctcgccgagc ggcttcaggt ggtggcgcgg ttttgcccgc
      121 aagaagtgca tgtgctggca cgggcggaca tcaagagcct cgccgatctc gccggcaaga
      181 aggtcaatat cgggcctgcc ggcggcagct cggcggtgac cgcgacgatc ctgttccgcg
      241 ccttcaacat ctcgatcgag ccgctcgctc tcgacggcca cgcagccatc gagcagttga
      301 agcaaggcac gatcgcagcc gccgtcattg tcggcgccaa gcccaccccg ctcatttccg
      361 agatttccgc gagcgacggc ctgcatctct tgccgatcga attcggcgcg ggcctcgaag
      421 aggcctatct gccgacaggc ttcgaccgcg acgattatcc gaacctcatc gcgatggaga
      481 cggagatccc gtcggtcgcg accggcctcg ttctgctcgc ggcggcgtcg aaggatgacg
      541 acggccacac cgagcgtctg gcggggttcg tcggtacgat gttttcgcgc ttcggcgagc
      601 tgaagaaaga cggccggcat ccgaagtggc gcgagatcaa tctcgccgcc agccttccgg
      661 ggtggacccg gaatccggcg gccgaagcat ggttgactga gcaacggaaa ggaagcgcgc
      721 gaagggaccc acaggtcagg atcgaagcgg gcgtagtccc gtcacatccg gcggtgggac
      781 aagaccagag ggaagcattg ttcaagcagt acatcgagtg gcagcgcgct aaggggcact
      841 agcgcactgc tttttacttg agggaggggg aaacggggcg cgttacgccg ggcggcttgc
      901 cgttcggcgg tgtaacagga gtaagataat gatcggttcc accacgccct gccaggcaga
      961 ggctggtttc cgaccgctgc gtgcacaact tgcccactct catgccgcgc tggcgcgtcc
     1021 ggcggcgatt tcgggcgaag ttcatcagag ggactatccg cagattgatt cgcagccgca
     1081 tccgcggcgc gaagctttcg ccatgacgct gccccgctcg ctcgcggcgc ggctgcgggc
     1141 gttcgccccg gacgacaagg agcttggcct ctttctcgat gaccttgccc tcgcggtgcg
     1201 caccgagaca ggcagcggct acgacatgag cgactcggcc cgcttcgaag ccggcctgcc
     1261 gctcgagccg caagtcgcaa gtcctgcgtc gcaagcgcat atgcaggccc tcgtccacga
     1321 cttccgccgg cgccagcgcc aggcaagcat gatcgtagcc ggctgcgtcg ccacctcctt
     1381 cgtgctggcg gtggtcggca tcgcggcgtt ggcaagcttc gtcaagcccg cccccgccga
     1441 cgccgagccg atgatcaaga gttcgaacgc cgacgtcctc caacaagacg ccgagccggc
     1501 gaagctcatc ctcgccaagg cggcgccctt taccgaccgc ttgccggtcg aggacgagga
     1561 caccgcgagc ctcgttcccg atcttggcgc gcctccggca tccgcagtca acgccgcgcc
     1621 gcagctgatc atggtgcagg ctggacgccc gctcgagctt gcccccctgc tctcgcagcg
     1681 gcaggcgcgc tacgtgcttc tccgcgggct ccccaatgaa gccacgctct ccgccggcca
     1741 gcgcaacccg agcggcgcat ggctcgtgaa ggacaaggat gtcgatcgcc tgacggtcac
     1801 catgcagagc acggcgagcg gcgactatcc catcgaggtt tacgcgttag gcgccagtag
     1861 cgccccccag gcccggcagc gtttagtgtt ccgcgtcgct gccgggccta ctgcgggcgt
     1921 ggcaaccggc gcgtccggcg tcctgttcaa catggcgctg ctcggcatgg cgccggagca
     1981 acctgccgca accaaagcct caccgctgat gacgcgcgcc atgcgcctgc tcggcgaggg
     2041 cgacattgcc ggtgcgcggc tcctgttcat gcatcttgcc gagcagggcg aaagcgaagc
     2101 cgcctacgag ctcgcgcgca ccttcgatgg cgaagttctg tcggagcttg gcgcgacagg
     2161 cgttggcgcc gaccggacgc gcgccgtggg ttggtatgaa cgcgcctcgg agacaggcaa
     2221 tgcaaaggcc gccgagcgct tgaagatcct cgctagtctc tcagactagc tagaggtggg
     2281 ccgcaaccgc taaccgaggg accaaatcgg ggggatcggc ggttgcggcc caacactctt
     2341 agacgacgtg ccggcgcctc agctcggcta gcagaagatc ggcgagcgca tccgcggtag
     2401 tattactggt gttgatcgca agctccggct gctccggcac ctcgtaatcc gaatcgatgc
     2461 cggtgaaatg gctgatcagc ccggcgcgcg ccttgcggta cagccccttc ggatcgcgcg
     2521 cctcgcatac ctcgatcggc gtctcgacat agacctcgac gaactcgccg tcctggaaca
     2581 gctcgcgcgc catgcgccg
//
LOCUS       DATNAE010000098         3585 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3409729, whole genome shotgun sequence.
ACCESSION   DATNAE010000098 DATNAE010000000
VERSION     DATNAE010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3409729"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1900)
                     /locus_tag="VN518_02090"
     CDS             complement(<1..1900)
                     /locus_tag="VN518_02090"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HXE03394.1"
                     /translation="MISDKIPRHEVVPAHALAADVVLQREGVNPALGLSEAEAASRLT
                     RYGPNALLAHAPRTALAILIDQFNSPVVWLLSAAAALAAAFSEWKEAAAILIVLVINT
                     LIGFFTELRAIRSMEALRKLGTRSSRVRRGGKLKTVAAKLVVPGDIVLLESGDVVTAD
                     IRLVEARNLSCDESTLTGESMPVEKSVHAVSADAILAERTSMLHKGTAITRGTGEGVV
                     VATGMATELGLTTRLVIEAESETSPLERKLAELTRQLVIAILIITALIAAAGMASGRD
                     LMLMIEASIALAVAAIPEGLPIVATMALARGMWRMAKRNVVVERLAAGATTVICTDKT
                     GTLTENRMTVERLRLTSGDYRLDHARAKILDDAGNEAELDAPLRRAIEVGVLCGTATL
                     ASSGDGGVGDPMELALQRFGRLAGLERAALLTASPRLHEIAFDTETKMMATAHRRDGH
                     LLVAVKGAPECVLQASTTLGEEKTLIAREDREEWLRRAAALAAEGLRVLALAESTIPL
                     EAVPTYESLNFLGLVGFRDPPRIDVKEAIADCRQAGIRVVMVTGDHASTARAIAEMLG
                     LAALGTTILVEGRELEALAALGERERARVLAAQVFARVTPAQKLELVRLYQSAGDVVA
                     MTGDGVNDA"
     gene            complement(1976..2545)
                     /locus_tag="VN518_02095"
     CDS             complement(1976..2545)
                     /locus_tag="VN518_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HXE03395.1"
                     /translation="MELLQAINGRRSVREYSAEPVDDALVHELIDAAVQAPSAINKQP
                     WCFIVVKRPKLLAEISDKAKAHMLKVSLNAPVHPISDMLSDPKFNIFYHAPVLIVIAT
                     AEPTDWAVEDCALAAQNLMLRAHDAGLGTCWIGFAQHWLGTPEGKAALDLPASYLPVA
                     PIIVGHPLRKPAPVPRKAPNIRWLGPTGQ"
     gene            complement(2637..>3585)
                     /locus_tag="VN518_02100"
     CDS             complement(2637..>3585)
                     /locus_tag="VN518_02100"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HXE03396.1"
                     /translation="QVGLETQGLTGGAAVTLRGGSAGTPLAPGEKGSPPMLIAAPLAS
                     QDWTEAARDTFQHVDTILSENSDSLHSAIKNIDTFAGALARNSDKVDGILAGIERMTG
                     GGTSQADIPTYDLVAASGFPPPAEAPGWLLVVPEPTTLMGFNTDKILLQPASGESVPL
                     GNARWSDNLPVLFQSKVIQSFENAGYAKEVSRTREGVTGNYQLLIDIRRFHIATSASP
                     EADIDFVAKLLDQDGKILAAHEFGATAPASGSDAKAYVSAFNDALGKLLTDLIDWSTK
                     AIAAAPPPAPPPPGEPSAPEAPPAEPPAPPQSEPPAPTP"
BASE COUNT          580 a         1120 c         1236 g          649 t
ORIGIN      
        1 gcgcatcgtt gacgccgtcg ccggtcatgg ccacgacgtc gccggccgat tgatagagcc
       61 tgacgagctc gagcttctgc gcaggagtca cccgggcgaa cacttgcgcc gcgagcacgc
      121 gggcgcgctc ccgttcgccg agcgcggcga gagcttccag ctcgcggccc tcgacgagaa
      181 tcgttgtgcc tagggcggcc aggccgagca tctcggcgat ggcgcgcgcg gtcgaggcat
      241 ggtcgcccgt gaccatgact acgcgaattc ctgcctgcct gcagtcggcg atcgcttctt
      301 tcacgtcgat gcgcggcggg tcgcggaatc cgaccagtcc gaggaaattc aggctctcat
      361 aggtggggac ggcctcaagc gggatcgtgc tctcggctag agcaaggacg cgcaacccct
      421 ccgcggctaa agcggcagcg cgtctcagcc attcctcgcg atcctcccgg gcgatgagcg
      481 tcttttcctc gccaagcgtc gtcgaggcct gaagcacgca ctcaggtgca cccttcacgg
      541 cgacgaggag gtgcccatcg cggcgatgcg cggtcgccat catcttcgtc tcggtatcga
      601 aggcaatctc gtgcagcctt ggagaggcgg tgagcaaggc ggcgcgctcc agtcccgcaa
      661 gccggccgaa cctttgcagc gctagctcca tcgggtcgcc tacgccgccg tcgccggaag
      721 aagcgagcgt cgccgtgccg cacaggacgc cgacctcgat ggcgcgccgg agcggggcat
      781 cgagctcggc ctcgttgccc gcgtcgtcga ggatcttcgc ccgcgcatga tcgagccggt
      841 agtcccccga tgtcagccga agccgctcga cggtcatgcg attttcggtg agggtgccgg
      901 tcttgtcggt gcagatgact gtggtggcgc ccgcggcgag gcgctcgacc acgacgtttc
      961 gctttgccat gcgccacatg ccgcgcgcga gcgccatggt cgccacgatc ggcaagccct
     1021 cgggaatggc ggcgaccgcg agtgcgattg acgcctcgat catgagcatg aggtctcggc
     1081 cagaggccat gccggccgcg gcgatcaggg cggtgatgat gaggatggcg atgacgagtt
     1141 gccgcgtcag ctcggcgagc ttccgttcga gcggggatgt ctcgctctcg gcctcgatca
     1201 cgagacgcgt ggtgagcccg agctcggtcg ccatgccggt cgccaccact acgccttcac
     1261 cggttccgcg ggtaatggcg gtgcccttgt gcagcatcga ggtccgctcg gcgaggatgg
     1321 cgtcggcgga gacagcgtgc acggactttt cgaccggcat ggattcgccg gtgagcgtcg
     1381 attcatcgca agagagatta cgcgcctcga caaggcggat atcggccgtg accacatcgc
     1441 cgctttcgag cagcacgatg tcgccgggca cgacgagctt ggcggcgacg gtcttgagtt
     1501 tgccgccccg ccgcacgcgc gaggatcgcg taccgagctt gcgcagcgcc tccatggagc
     1561 ggatggcgcg cagctcggtg aagaagccga tcagggtgtt gatgacgagc acgatgagga
     1621 tggcggcggc ttctttccac tcgctgaagg cggccgcgag cgctgctgcc gctgacagca
     1681 gccacaccac ggggctgttg aattggtcga tgagaatggc caacgcggtc cgcggtgcgt
     1741 gggcaaggag cgcattcggc ccgtaacgtg tgagccgcga ggccgcttcc gcctcgctga
     1801 gcccaagagc cgggttcacg ccctcgcgct gcagcacgac atccgcggca agcgcgtggg
     1861 cgggcaccac ttcgtggcgc gggatcttgt ccgatatcat cagccgctcc gttgtcgcgg
     1921 tgatgttatc gccatcggat gagggaatag tgcgcctgcg cctcgtggtc acgcgttatt
     1981 ggccggtagg gcctagccac ctgatgtttg gggccttgcg gggaacgggc gcaggcttgc
     2041 gaagcggatg tccgacgatg atcggggcga ccggaagata ggacgccggc aggtcaagcg
     2101 cggccttccc ctctggcgtt ccgagccaat gctgggcaaa gccgatccag cacgtgccga
     2161 gccctgcgtc atgggccctc agcatcaggt tttgcgcggc gagagcgcaa tcctcgaccg
     2221 cccagtccgt cggctcggcc gtcgcgatca cgatcagcac cggcgcgtga tagaagatgt
     2281 tgaacttcgg atcgctcagc atatcgctga tgggatggac cggcgcgttg agagagacct
     2341 tgagcatgtg ggccttggct ttatcagaga tctcggcgag taatttcggg cgcttgacga
     2401 cgatgaagca ccaaggctgc ttgttgatgg cacttggcgc ctgaaccgcg gcgtcgatca
     2461 gctcgtgcac cagagcgtcg tcaacgggct cggcggaata ttcgcgcacc gaccggcggc
     2521 cattgatggc ttgcaatagt tccattgccg cgaccagctc catcccgtga tggggcgata
     2581 gaatgagtgg cttcaaccac gatcccgctc gcagccgcat tccgcgttga gccagatcaa
     2641 ggagttgggg caggaggctc gctttgcggc ggtgccggcg gttcggcggg aggcgcttcc
     2701 ggggccgacg gttcgcccgg tggcggcggc gccggtggcg gcgcggcggc gattgccttc
     2761 gtgctccaat cgatcaggtc ggtcagcaac ttgcctaagg catcattgaa tgcgcttaca
     2821 taggctttcg catcgcttcc cgaggcaggg gcggttgcgc cgaactcgtg cgcggcgagg
     2881 atcttgccat cctgatcgag cagcttcgcc acgaagtcga tatcggcctc aggactggcc
     2941 gaggtcgcga tatggaagcg tcggatatcg atcagaagct gataattgcc ggtgacgcct
     3001 tcacgcgtcc ggctgacttc ctttgcgtag cccgcattct cgaagctctg gatcaccttg
     3061 gattggaaga gaacggggag gttgtcgctc caacgcgcat tgccgagggg cacgctttcc
     3121 ccgctcgccg gctgaagcag gatcttgtcg gtgttgaagc ccatcagcgt cgtcggctcg
     3181 ggcaccacga gaagccagcc gggcgcctcc gcaggaggag ggaagccgct cgcggcgaca
     3241 agatcgtaag tgggaatgtc tgcctggctc gtgccccctc cggtcatgcg ctcgatgccg
     3301 gcaaggatgc cgtcgacctt gtcggagttc cgcgccagcg cgccagcgaa agtgtcgata
     3361 tttttgatgg cgctgtgcaa ggagtcggaa ttctcggaga ggatggtgtc gacgtgctgg
     3421 aaagtgtcgc gcgccgcctc ggtccagtcc tggctcgcga gcggtgctgc gatcaacatg
     3481 gggggcgagc ctttttctcc cggcgcaagt ggcgtgccgg cgctaccgcc tctcagcgtc
     3541 accgcggcgc cgccggtgag cccctgcgtt tccaggccga cctgg
//
LOCUS       DATNAE010000099         2159 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_1609313, whole genome shotgun sequence.
ACCESSION   DATNAE010000099 DATNAE010000000
VERSION     DATNAE010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1609313"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="VN518_02105"
     CDS             <1..820
                     /locus_tag="VN518_02105"
                     /inference="COORDINATES: protein motif:HMM:NF021506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HXE03397.1"
                     /translation="VSALIGTVVVAASLGPAMAAIFAVSIGAPMVLLVHLALLSRPVT
                     TPQGQVVGALEWYPPGRLVGWAALIAGLLAGLLVLILGYDQDSYRDSIREILNHSALK
                     ELDRDGTLFTEENIANLSAMIARALPAAFAIIWLTITLFNLWMAGLIVDASGRALRPW
                     PNLHALELPNALLLVFAAALAASFLPGLAGLLATGLAGALLFAYVLQGLAVIHVYSRG
                     VSLRPLLLATVYISILLLGWVAIIVAIVGLAEPLINLRHRPGPGPQPPRNNGTM"
     gene            841..1452
                     /gene="rplI"
                     /locus_tag="VN518_02110"
     CDS             841..1452
                     /gene="rplI"
                     /locus_tag="VN518_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HXE03398.1"
                     /translation="MQVILLERIGRLGQMGDVVNVKDGFARNYLLPQGKALRATEANR
                     KRFERDKAQLEARDLELKSEAQAVATKLDRKSFIIIRQAGESGQLYGSVSTRDIAAAV
                     TEGGFSIERRQVMLDRPIKTLGLHDIRVALHAEVQPHIAVNVARSADEAARQARGEDV
                     TRAMTEAEEDAEAARVAAEALFEEGAPAEIAGEEEAEDEAADA"
     gene            1557..>2159
                     /locus_tag="VN518_02115"
     CDS             1557..>2159
                     /locus_tag="VN518_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HXE03399.1"
                     /translation="MSNALIRPLQPETPVFREAPHNLEAEQALLGAILVNNEAIDRVS
                     TFLKPGHFYDALHARIYEAASKLILSGKRATPITLKTFFQSDPPVGELPVPQYLGRLA
                     ANATTIINAEDYGRTVYDLAIRRQLIQIGEEMVNTAFDSPIEAPPSVQIEGAEQQLYE
                     LAETGKYGTGFEPFSSALTDAIDMAANAYRRDGGLSGLATG"
BASE COUNT          358 a          757 c          664 g          380 t
ORIGIN      
        1 cgtctcggct ctgatcggca cggtggtcgt ggccgcaagt ctcggcccgg ccatggcggc
       61 catcttcgcc gtctccatcg gcgcgccgat ggtgctcctc gtgcatctcg cgctgctatc
      121 gcggcccgtc accacgccgc agggacaagt cgtcggcgca ctcgaatggt atccccccgg
      181 ccgcctcgtc ggttgggcgg cgctgatcgc ggggctgctc gccggcctgc tggtgctgat
      241 cctgggctac gatcaggaca gctatcgcga ctcgatccgc gagatcctca accacagcgc
      301 attgaaggaa ctcgatcgcg atgggacgct gttcaccgag gagaacatcg ccaatctctc
      361 ggccatgatc gcccgggcgc tgcccgccgc cttcgccatc atctggctca ccatcacgct
      421 gttcaatctg tggatggccg ggctgatcgt cgacgcctcg gggcgtgcgc tcaggccctg
      481 gcccaatctg catgcgctcg agcttcccaa cgctttgctg ctcgtgttcg ccgcagcgct
      541 tgctgcatct ttcttgccgg ggctggcggg cttgctcgcg accgggctcg ccggcgcgct
      601 gctgttcgcc tatgtcctgc aggggctcgc ggtgatccac gtctatagcc gcggcgtgtc
      661 gcttcgcccg cttctgctgg caacggtcta tatcagcatt ttgctgctgg gttgggtggc
      721 catcatcgtc gccatcgtcg gccttgccga gcctctcatc aatttgcgcc accgccccgg
      781 ccctggcccc cagccccccc gcaacaacgg aactatgtga caaggatagg agtgtcgaac
      841 atgcaagtca ttctgctcga gcgcatcggg cgcctcggac aaatgggcga tgtcgtcaac
      901 gtcaaggacg gcttcgcccg caactacctg ctcccccaag gcaaggccct gcgcgccacc
      961 gaggccaatc gcaagcgctt cgagcgcgac aaggcccagc tcgaggcccg cgatctcgag
     1021 ctcaaatccg aggcccaggc cgtcgcgacc aagctcgacc ggaagagctt catcatcatc
     1081 cgtcaagccg gtgagagcgg ccagctctac ggctcggttt ccactcgcga cattgccgcc
     1141 gccgtcaccg agggcggctt ttccatcgag cgccgccagg tgatgctcga ccgtccgatc
     1201 aagacgctcg gcttgcacga cattcgcgtc gcgctgcacg ccgaggtgca gccgcatatc
     1261 gcggtcaatg tggcgcgctc cgccgacgag gcggcacgcc aagcgcgcgg cgaggacgtg
     1321 acgcgggcca tgaccgaggc cgaggaggat gccgaagccg caagagttgc ggcagaggcg
     1381 ctgttcgagg agggcgcgcc cgccgagatc gccggcgaag aggaagccga ggacgaagcg
     1441 gcagacgcct aaccgacgcg atgcgcgctt tgctccctgt gggcgagcgc acgctctcca
     1501 cgttgtccac agaccggacg gctagcccgg agtccggatt ggggtagtct cggtgcatga
     1561 gcaacgcatt gattcgcccg ctgcagccgg aaactccggt gtttcgcgag gcgccgcaca
     1621 acttggaggc cgaacaggcc ctgctcggcg ccatcctcgt caacaacgag gcgatcgacc
     1681 gcgtctcgac cttcctcaag ccaggccatt tctacgatgc attgcacgct cgcatctatg
     1741 aagcggcctc gaagctgatc ctgagcggca agcgcgccac gccgatcacg ctgaagactt
     1801 tcttccagtc cgatccgccg gttggcgaac tgcccgtgcc gcaatatctc gggcggctcg
     1861 cggccaatgc caccaccatc atcaatgccg aggattacgg tcgcaccgtc tacgacctgg
     1921 ctatccgccg ccagctgatt cagatcggcg aggagatggt caacacggcc ttcgattcgc
     1981 cgatcgaggc gcctccctcc gtccagatcg aaggcgccga gcagcagctt tacgagctcg
     2041 ccgagaccgg caaatacggc acgggcttcg aaccgttctc ctcggcgctg accgacgcca
     2101 tcgacatggc agccaacgct tatcggcgcg acggcggctt gtccggcctt gccaccggc
//
LOCUS       DATNAE010000100         2186 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_1001651, whole genome shotgun sequence.
ACCESSION   DATNAE010000100 DATNAE010000000
VERSION     DATNAE010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1001651"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..558
                     /locus_tag="VN518_02120"
     CDS             157..558
                     /locus_tag="VN518_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HXE03400.1"
                     /translation="MHLLHRAGQCAADIFASEARTSGLTPRQFAVLTIVAEEEGLTQT
                     DLVERTGIDRSTLADIVGRLLGRGLIQRRRAKDDARAYSIKLTPQGAKVLREAQPGAA
                     AADQKLLASLPPAKRQEFLETLNLIVTAIRK"
     gene            complement(574..1146)
                     /locus_tag="VN518_02125"
     CDS             complement(574..1146)
                     /locus_tag="VN518_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014891441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HXE03401.1"
                     /translation="MSEATRAPALRRFGLALPLVLFAVLAGLFWYALHAGDPSLLPSP
                     LIGKPVPSFTLPPIDGLSADGEAVPGFASGDLAQGEPTIVNVFASWCVECQVEHPALL
                     ALSQEPGVRLYGIDYKDDMASARRFLGRNGNPYARVGADASGRTAIDFGVYGVPETYV
                     VTGDGKIAYRHVGPLTESAIRDKILPLLAH"
     gene            complement(1143..1355)
                     /gene="ccmD"
                     /locus_tag="VN518_02130"
     CDS             complement(1143..1355)
                     /gene="ccmD"
                     /locus_tag="VN518_02130"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006023240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HXE03402.1"
                     /translation="MLDLGPHASFIIAAYAVTLVAVAALAFFIVEDDRKQRRILAELE
                     RKGIRRRSAEPKPTPGKPKSKRKTGA"
     gene            complement(1359..2096)
                     /locus_tag="VN518_02135"
     CDS             complement(1359..2096)
                     /locus_tag="VN518_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease"
                     /protein_id="HXE03403.1"
                     /translation="MISLANPTRFLGLVDRVVPWLTAVTLILLGLGLYLSLFVAPPDY
                     QQGETVKIMFIHVPSAWLAMFGYMLIALAALGTLIWRHPLADVAAKTAAPIGATFTFV
                     ALVTGSLWGKPMWGTYWVWDARLTSVLILFLLYLGLIALWQSIEEPGRAGRAAAILAL
                     VGAVNVPIIHYSVVWWNTLHQPASVFRLGGPSIAPALLTPLLVMAIAFTLLFVLLHLM
                     AMRAEILRRRVRALQQTQVERAALVEA"
BASE COUNT          415 a          708 c          738 g          325 t
ORIGIN      
        1 gcaacctctc gtttgcggtt ctctggtctg agttgtatag gtgaggtcaa agcgccttga
       61 tggccaaggg cagcccattg gagatacacg ctctacgcga gttgagcgtg gctcaatatg
      121 gttaagggag cgagagacca cctcgaccgc tccgcgatgc acctcctcca tcgcgcgggt
      181 cagtgtgccg ccgacatctt cgcgtcggaa gcgaggacga gcgggcttac gccccggcaa
      241 ttcgcagttc tcaccatcgt tgccgaggag gagggtctga cccagaccga cctcgtcgag
      301 cggaccggca tcgaccgctc cacgcttgcc gatatcgtcg gtcgcctgct cggccgcgga
      361 ctgatccagc gccgccgcgc caaggacgac gccagagcct attcgatcaa gctgacgccg
      421 cagggcgcca aggtgctgcg cgaggcgcaa cccggcgccg ccgccgccga ccagaagctg
      481 ctggcgagcc ttcctcccgc caagcggcag gagttcctcg agacgctcaa tctgatcgtc
      541 accgccatcc gcaaatagcg tttcgctccg ctctcaatgc gcgagcagcg gcaggatctt
      601 gtccctgatc gcgctctcgg tgaggggacc gacatggcgg taggcgatct tgccgtctcc
      661 ggttacgacg taagtctcgg gcacgccata cacgccgaaa tcgatggccg tgcggccgga
      721 ggcgtcggcg ccgacccttg cataaggatt gccgttgcgg ccgaggaagc gccgcgccga
      781 cgccatgtcg tccttatagt cgatgccata gagcctgacg ccgggctcct ggctcaacgc
      841 cagcagcgcc ggatgctcca cctggcactc cacgcaccac gaggcgaaca cattgacgat
      901 ggtcggctcg ccctgcgcga ggtcgcccga ggcgaagccc ggcaccgcct cgccgtccgc
      961 gctcaatccg tcgatgggcg gcagcgtgaa gctcggcacc ggcttgccga tcaagggcga
     1021 tggcagcagc gaaggatcgc cggcatgcag cgcgtaccag aacaggccgg cgaggacggc
     1081 gaagagcacg agcggcagag ccagcccgaa acgccgcagt gcgggagcgc gggtcgcctc
     1141 gctcatgcgc cggtcttccg cttcgatttc ggcttgcccg gcgtgggctt gggctcggcc
     1201 gagcgccgcc taatgccctt gcgctcgagc tcggcgagga tgcgccgctg cttgcgatcg
     1261 tcctcgacga tgaaaaaggc aagcgcggcc accgccacca aggtcacggc ataggcggcg
     1321 atgatgaaag aggcgtgcgg gccgaggtcg agcatggctc aagcctcgac cagagcggcg
     1381 cgctcgactt gcgtctgctg caacgcgcgc acgcggcggc gcaagatctc cgcgcgcatg
     1441 gccatcaggt gcagcagcac gaacagcagg gtgaaggcaa tggccatgac caagagcggg
     1501 gtcagcaggg ccggagcgat gctcgggccg ccaagcctga acacgctcgc cggctgatgc
     1561 agcgtattcc accacaccac cgagtaatga atgatcggca cattgacggc gccgaccagc
     1621 gcgaggatgg cggcggcgcg gccggcacgt cccggctctt cgatcgactg ccacagggcg
     1681 atcagcccga gataaagcag aaagagaatg agcaccgagg tcagccgcgc gtcccatacc
     1741 caataggtgc cccacatcgg cttgccccac agcgagccgg tgacgagcgc gacgaaggtg
     1801 aaggtggcgc cgattggcgc cgcggtcttg gcggcgacgt cggcgagcgg atggcgccag
     1861 atcaacgtgc cgagcgctgc gagcgcgatc agcatatagc cgaacatggc gagccaggcc
     1921 gacgggacgt ggatgaacat gatcttcacc gtctcgccct gctgataatc gggcggcgcg
     1981 acgaagagcg agaggtaaag cccgagcccg agcaggatca aggttacggc cgtgagccag
     2041 gggacgacgc ggtcgaccag gcccaaaaac cgggtcggat tggcgaggct gatcatcggg
     2101 cttgatctag tgggtttggc tggtgagcgc aattgcggcc ctccgcagaa taagggcagc
     2161 gtgggccgag cagttcctct tgctgc
//
LOCUS       DATNAE010000101         2980 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3111319, whole genome shotgun sequence.
ACCESSION   DATNAE010000101 DATNAE010000000
VERSION     DATNAE010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3111319"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /gene="nuoI"
                     /locus_tag="VN518_02140"
     CDS             complement(<1..425)
                     /gene="nuoI"
                     /locus_tag="VN518_02140"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HXE03404.1"
                     /translation="MEFLSAFWLAMKYFVAPKATLNYPFEKGPLSPRFRGEHALRRYP
                     NGEERCIACKLCEAICPAQAITIEAGPRRNDGTRRTTRYDIDMVKCIYCGFCQEACPV
                     DAIVEGPNFEFATETREELYYDKERLLANGDRWEREIAK"
     gene            complement(478..1500)
                     /gene="nuoH"
                     /locus_tag="VN518_02145"
     CDS             complement(478..1500)
                     /gene="nuoH"
                     /locus_tag="VN518_02145"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HXE03405.1"
                     /translation="MAEFWTSYGLPLALIVAQSVALLVVLLIIVAFLLYADRKVWAAV
                     QMRRGPNVVGPWGLWQSFADLLKFVFKEPIIPAGANKGIFLLAPLVTAGLALSAWAVI
                     PVAKGWEVADLNVGILFVFAISSLQVYGVIMAGWASNSKYAFLSALRSAAQMVSYEVS
                     IGFVIITVLLCAGSLNLSAIVEAQDGRFGLFGWYWLPLFPMFVIFFISALAETNRPPF
                     DLLEAESELVAGFMVEYSSTPYMLFMLGEYVAIVLLCGLGTVLFLGGWLPPVPLPPFT
                     WIPGVVWFLLKVCFLFFLIAMVKAMVPRYRYDQLMRLGWKVFLPLSLAAVVLVAAVLQ
                     LWQHAS"
     gene            complement(1493..>2980)
                     /locus_tag="VN518_02150"
     CDS             complement(1493..>2980)
                     /locus_tag="VN518_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018391217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HXE03406.1"
                     /translation="LTSKPYAFMARPWELSKTLSIDVMDALGSAIRVDARGRDVMRIL
                     PRVNDAINEEWISDKARHVWDGLRTQRLDRPYVRKQGKLVPASWDEAFRVIAERLKGL
                     DGSRFAAIAGDLAAAEEIFALKLLSEQIGSPNIDCRQDGAALDPALGRATYLFNSTIA
                     GIDQADAILLIGTDPRHEAPVLNARILKRWRQTQGKIPIGLIGEDADLTYAYDYLGAG
                     PETLAKIADGSRAFMKMLEGVERPMLILGQSALARPDGAAVLALAAKAAVTMGAIKPA
                     ISWNGFNVLHHAAGRVAGLDLGLVPAPGGLDMNAILDAAGAGTIEVVYLLGADEIDMA
                     CLGKALVIYQGSHGDRGAHRADVILPGAAYTEKDSIYVNTEGRAQMTARAVFPPGEAR
                     EDWTILRALSGVLGKPLPFDSTQALRAALFEAHPHLALLDQIDPADSAAIERLAKQAA
                     KPGRERFGHAIGDFYLTNPIARASAIMASLSALHTSPDKATGTDG"
BASE COUNT          600 a          920 c          976 g          484 t
ORIGIN      
        1 ttcttggcga tctcgcgctc ccagcggtcg ccgttcgcca gcagccgctc cttgtcgtag
       61 taaagctcct cgcgcgtttc ggtggcgaat tcgaaattcg gaccctcgac aatggcgtcc
      121 accgggcagg cctcctggca gaaaccgcaa tagatgcatt tcaccatgtc gatgtcgtag
      181 cgcgtggtgc ggcgcgtgcc gtcgttgcgg cggggtccgg cctcgatggt gatagcctgg
      241 gccggacaga tggcctcgca gagcttgcag gcgatgcagc gctcctcgcc gttgggataa
      301 cgccgcagtg catgctcacc gcggaagcgg ggcgagagcg gccccttctc gaaggggtag
      361 ttgagggtcg ccttgggagc cacgaaatat ttcatggcga gccagaacgc tgacagaaat
      421 tccatcagca acaatgactt ggcggcctcg gcgaagcgct tcacgcgagc tctcctctta
      481 ggatgcgtgc tgccagagtt gcagaaccgc cgccacaagc acgacagcgg ccagtgacag
      541 tggcaggaag accttccagc cgagccgcat caactgatcg tagcggtagc gcggtaccat
      601 ggctttgacc atggcgatca ggaagaacaa gaagcaaacc ttcagcagga accataccac
      661 gcctggtatc caggtgaagg gtgggagggg aaccggcggt agccagcccc ccaaaaacag
      721 caccgtgccg aggccgcata acaggacgat ggcgacatat tcgcccagca tgaaaagcat
      781 atagggcgtg gacgagtatt cgaccataaa gccggcgacg agctcagact cggcctccag
      841 caggtcgaaa ggcgggcggt tggtctcggc cagagccgaa atgaagaaga tcacgaacat
      901 cggaaagagc ggcagccaat accagccgaa caacccgaaa cgcccgtctt gcgcctcgac
      961 gatggcgctc aaattgagcg agccggcgca cagcagcacg gtgatgatga cgaagccgat
     1021 cgacacttcg tacgagacca tttgcgccgc cgagcggagc gccgagagaa aggcgtattt
     1081 cgagttcgag gcccacccgg ccatgatcac gccgtagacc tgaaggctgg agatggcgaa
     1141 gacgaaaagg atgccgacat tgaggtcggc gacctcccag cccttggcga ccggtatgac
     1201 ggcccaagcc gacagcgcga gccccgccgt gacgagtggc gcgagcagga agataccctt
     1261 attggcgccg gccggaatga tcggctcctt gaagacgaat ttcagcaggt cggcgaagga
     1321 ctgccacagt ccccaggggc cgaccacatt aggcccgcgc cgcatctgca ccgccgccca
     1381 gaccttgcgg tcggcataaa gcagaaaggc aacgatgatc agaaggacaa cgagcagcgc
     1441 cacgctctgc gccacgatca gcgcgagcgg caggccatag ctcgtccaaa actcagccat
     1501 cagtgccggt cgccttgtcg ggggacgtgt gcagcgcgct caagctcgcc atgatggcgg
     1561 aagcgcgcgc gatgggattg gtgagataga agtcgccgat cgcatggccg aaacgctccc
     1621 tgcctggctt cgctgcttgc ttggcgagac gctcgatggc cgcgctgtcg gcggggtcga
     1681 tctggtcgag cagagcgaga tgcgggtgcg cttcgaacag cgcggcccgc agcgcctgag
     1741 tcgagtcgaa tggcagaggc ttgccgagca cgcccgagag tgcccgcagg atggtccagt
     1801 cctcgcgcgc ttcacccggc gggaacacgg cgcgtgcggt catctgtgcc cggccttcgg
     1861 tattcacgta gatggagtcc ttctcggtat aggcggcgcc gggcaggatg acgtcggcgc
     1921 gatgggcgcc tcgatcgccg tggctgccct ggtagatgac aagcgccttg ccgagacatg
     1981 ccatgtcgat ctcgtcggct ccgagcagat agacgacctc gatggtcccc gcccctgccg
     2041 catcgagtat ggcgttcata tcaaggccac ctggcgccgg aacgaggcca agatcgagcc
     2101 cggccacgcg tccggcggcg tggtgcagca cgttgaagcc gttccaggag attgccggct
     2161 tgatagcgcc catcgtcacc gcggccttgg cggcgagggc caagaccgcc gcgccatcgg
     2221 gccgcgccag cgccgactga cccaggatca acatcggccg ctcaacgcct tcgagcattt
     2281 tcatgaaagc acgggagccg tcggcgatct tggcgagtgt ctcgggaccg gcgccgagat
     2341 aatcgtaagc ataggtcagg tcggcatcct caccgatcag cccgatgggt attttgcctt
     2401 gagtctggcg ccagcgcttg aggatacgcg cattcagcac gggcgcctca tgccgcggat
     2461 cggtgccgat gagaaggatc gcatcggcct ggtcgatgcc ggcgatggtg gagttgaaaa
     2521 gataggtcgc ccgtccaagc gcgggatcga gtgctgcgcc atcctggcgg caatcgatat
     2581 tcggcgagcc gatctgctcg gagagcagct tcagcgcgaa gatctcctcc gcggcggcca
     2641 gatcgccggc gattgccgca aagcgcgagc cgtcgagccc tttcagtcgc tcggcgatga
     2701 cgcgaaaggc ctcgtcccag ctcgccggaa cgagcttccc ctgctttcgc acataagggc
     2761 ggtcgagacg ctgagtacgc aggccgtccc agacatggcg cgccttgtcg gagatccatt
     2821 cctcgttgat ggcgtcgttc acgcgcggca ggatgcgcat cacgtcccgt ccgcgtgcat
     2881 cgacgcgaat ggcgctgccg agcgcgtcca tgacatcgat cgagagcgtc ttgctgagct
     2941 cccagggccg cgccatgaag gcgtaaggct tcgaggtgag
//
LOCUS       DATNAE010000102         3751 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_4316055, whole genome shotgun sequence.
ACCESSION   DATNAE010000102 DATNAE010000000
VERSION     DATNAE010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4316055"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            270..2222
                     /locus_tag="VN518_02155"
     CDS             270..2222
                     /locus_tag="VN518_02155"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HXE03407.1"
                     /translation="MKKLLIGIAVVIVLVIVAAIAIPFFIPVDTYKGQLIAQVKQATG
                     RDLRIDGKMSFSLIPNIALEANDVSFSNPPGAAAKDMAKLGKLQVQLKFWPLLHKDIE
                     VDRLVLVDPTIDLEVDKQGKPNWQFASSAAPAQPPAAPAGATAPSSGGTGGSLAQLRL
                     DDVRLVNGHVTYVDQRTGDKKELTEIGMKLSLPGLDQPFSADGSAVWNKEKVSLNASF
                     ANLRAFMDGKSSGAALKVAAKPVSFDFKGNASGGVPLKLDGTVDLQVPSVRQLAAWAG
                     SPLTAPGTGFGPLAISGKVAYAGPKITFSDANLSLDNIKAKGEIAVDTAGARPAIKGQ
                     LAVDKLDVNPYLPPENAANAPAAPSGGGGGATGANAKSDWSDDPIDVSGLKAGDLEFQ
                     LSANAIQYRKIQVGKSALHLDLKGGRFEADLTELALYQGAGKGKVVLDGSGAVPAIDT
                     SFNLNNVQMQPLLKDAAGTDRLGGAGAFDMAVTGKGKSQREIVSSLNGKGDLNLANGV
                     IKGVNLVGMVKNIASALQGGDAQETDFASLTATYTITNGVLRNNDLALKSAEVPMTGA
                     GTVDLPHRTVDYKITPKLAGALAVPIIVKGPWDHLSYQPDLAGMVGDPSKLIQGGAKG
                     VGDALKQQQPGVDNLLKGFLGGKKQQ"
     gene            complement(2234..3112)
                     /locus_tag="VN518_02160"
     CDS             complement(2234..3112)
                     /locus_tag="VN518_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020607881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HXE03408.1"
                     /translation="MMKPRDRVAYSAIVDRPPLKLPDGLRLIVWPIVNLEEWEIERPM
                     ARQVLPPPTGVTQIPDVPNWSWHEYGMRVGVWRFFAALDRVGAKATLSINAKVVESYP
                     RVAEAARERGWEFMGHSFVQGPIHKLEDQRATIHQSLDLLQRFTGKRPVGWLGPGLTQ
                     TYETPELLAEAGVQYIGDWVYDDEPTEIRTERGPLVTLPYTVELNDIPMMIVQHHPAD
                     EFLRRSVDQFDRLYSESAERAKILSFAIHPYISGAPHRIKYLEEFLDYARRHTGVAFW
                     TGEDILAWYRKVKPKA"
     gene            complement(3189..>3751)
                     /locus_tag="VN518_02165"
     CDS             complement(3189..>3751)
                     /locus_tag="VN518_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HXE03409.1"
                     /translation="PEGYLKAPPDRLERWRHRFAEPSRALRIGLAWAGNAEQSNDRNR
                     STSLASFLPLLDIEGTEFYSLQVGARAAEIGTLPGARRLVDLSFELSDFAETAAAMTV
                     LDLIVTVDTSVAHLAGALGRPAFVALTYLPDWRWYLTGSDNPWYASLRVFRQPSRGDW
                     ASVVTAIAATLRERVAERATAAAPSA"
BASE COUNT          699 a         1186 c         1261 g          605 t
ORIGIN      
        1 cgagtgggcc ggaattggct tctcgcttcg tggagaaagg accgcagggg gcagacgaaa
       61 attcaccacg aaattttttc cggcttggcg aataaaggcg tgaagcgacg ggtccgcgac
      121 gccatcagcc gacgaggcgg acggtcacag ccgatcttgc tgagatttca agaaatgggg
      181 tcgcgggaat ggaggcgtct acacggcgtt aggtctacga actagagttg cggggtgcgg
      241 catttgacga cggcatcggg aggcacgtca tgaaaaagtt gttgatcggc atcgcggtgg
      301 tcatcgtcct ggtgatcgtc gcggcgatcg ccatcccctt cttcattccg gtcgacacct
      361 acaagggcca gctcatcgcc caggtcaagc aggcgaccgg ccgcgacctg cgcatcgacg
      421 gcaagatgtc cttctcgctc attcccaata tcgcgctcga ggccaacgac gtctcattct
      481 ccaatccgcc gggcgctgcg gccaaggaca tggcaaaact cggcaagctg caggtgcaac
      541 tcaaattctg gccgctgctg cacaaggaca tcgaagtcga ccgcctggtg ctggtcgatc
      601 ccacgatcga cctcgaagtc gacaagcagg gcaagcccaa ttggcagttt gcctcgagcg
      661 cggcgccggc gcagccgcct gccgcgccgg ccggggcaac cgcgccgtcc tccggcggca
      721 cgggcggcag cctcgcgcag cttcgcctcg acgatgtccg tctcgtcaac gggcatgtga
      781 cctatgtcga ccagcgcacc ggcgacaaaa aggagctgac cgagatcggc atgaagctgt
      841 cgctgcccgg cctcgatcag cccttcagcg ccgatggctc ggcggtgtgg aacaaggaga
      901 aggtttcctt gaacgcctcc ttcgccaatc tgcgcgcctt catggacggc aaaagcagcg
      961 gggccgcgct caaagtcgcg gcgaagccgg tgagcttcga cttcaagggc aatgcctcgg
     1021 gcggcgtgcc gctgaagctc gacggcacgg tcgatctgca ggtgccgtcg gtgcggcaat
     1081 tggcggcctg ggcgggaagc ccgctcactg cgcccggcac cggcttcggt cccttggcaa
     1141 tctccggcaa ggtcgcctat gccggcccca agatcacctt cagcgatgcc aatctctcgc
     1201 tcgacaacat caaggccaag ggcgaaatcg cggtcgacac cgccggggcg cggccggcga
     1261 tcaaaggcca actcgctgtc gacaagctcg acgtcaatcc ctacctgccg ccggagaacg
     1321 ccgcgaatgc gccggcggcg cccagtggcg gcggcggtgg cgcgacgggt gccaacgcca
     1381 agtcagattg gagcgatgat cccatcgatg tcagcggcct caaagccggc gatctcgaat
     1441 tccagctcag cgccaatgcc atccagtatc gcaagatcca ggtgggcaaa agcgccctgc
     1501 atctggactt gaagggcggc cgcttcgagg ccgatctcac cgagctcgcg ctctatcagg
     1561 gcgccggcaa gggcaaggtt gtcctcgacg gcagcggcgc cgtgcccgcg atcgacacca
     1621 gcttcaactt gaacaatgtg cagatgcagc cgctgctcaa ggacgccgcc ggcaccgatc
     1681 gtctcggcgg cgccggcgcc ttcgatatgg cggtcactgg caaaggcaaa agccagcgcg
     1741 agatcgtctc ctcgcttaac ggcaagggcg acctcaacct cgccaatggc gtgatcaagg
     1801 gcgtgaacct cgtcggcatg gtcaagaaca tcgcctcggc gctgcaaggc ggcgatgcgc
     1861 aggagacgga tttcgcctcg ctcaccgcca cctacaccat caccaatggc gttttgcgca
     1921 acaacgatct cgcgctcaaa tcggcggaag tgccgatgac cggcgcaggg acggtcgacc
     1981 tgccgcatcg cacggtcgac tacaagatca cgcccaagct cgctggtgcc ctggcagtgc
     2041 cgatcatcgt caaggggccg tgggatcatc tgagctacca gcccgacctc gcgggcatgg
     2101 tcggcgatcc ctcgaagctc atacaaggcg gtgccaaagg cgtcggcgat gcactgaagc
     2161 agcagcagcc cggtgtcgac aatctcctga aaggcttcct cggcggcaag aagcagcagt
     2221 agcgccgccc gcctcaggct ttgggtttca ccttgcggta ccaggcgagg atgtcttcgc
     2281 cggtccagaa ggcgaccccg gtgtggcggc gcgcatagtc gaggaattcc tcgaggtatt
     2341 tgatgcggtg cggcgcgccg ctgatatagg ggtggatggc gaaggagagg atcttggcgc
     2401 gctcggcgct ttcgctgtag agccggtcga actggtcgac cgaacggcgc aggaactcgt
     2461 cggcgggatg atgctggacg atcatcatcg ggatgtcgtt aagctccact gtatagggca
     2521 gcgtcaccaa cggcccgcgc tcggtccgga tctcggtcgg ctcgtcgtca tagacccaat
     2581 cgccgatata ttggacgccc gcctcggcga ggagctcggg cgtctcgtag gtctgggtga
     2641 gaccgggccc cagccagccc accggccgct tgccggtgaa gcgctgcagc aggtcgagcg
     2701 attggtggat ggtcgcgcgc tggtcttcga gtttgtggat cgggccctgg acgaaggaat
     2761 ggcccatgaa ttcccagccg cgctcgcgcg cggcctcggc gacgcgcgga tagctctcga
     2821 ccaccttggc attgatcgag agcgtcgcct tggcgccgac gcggtcgagc gccgcgaaga
     2881 agcgccagac gccgacgcgc atgccgtatt cgtgccagct ccaattgggc acatcaggga
     2941 tttgcgtgac gccggtcggc ggcggcagca cttggcgcgc catcggccgc tcgatctccc
     3001 attcctcgag attgacgatc ggccagacaa tgaggcgcaa gccatcgggc agcttcagtg
     3061 gcgggcgatc gacaatggcg gaataggcga cgcggtcacg cggcttcatc attcgctcac
     3121 tcgctgcaag ggtcgggccg gcagcatatc cggcagggcg ctcgcggcaa gcggcaacgg
     3181 agaaagggct aggcggacgg tgcggcggcg gtggcgcgtt cggcaacgcg ttcgcgcagc
     3241 gtcgcggcga tcgccgtcac cacgctcgcc caatcgccgc ggctcggctg gcggaagaca
     3301 cgcaggctcg cgtaccaggg attgtcggag ccggtgaggt accaccgcca atccgggagg
     3361 taggtcagcg ccacgaaagc cgggcgcccg agcgcaccgg cgagatgggc gaccgatgtg
     3421 tcgacggtga cgatgaggtc gaggacggtc atcgccgcgg ccgtctcggc gaaatcgctg
     3481 agttcgaagc tgagatcgac aaggcgccga gcgccgggga gcgttccgat ctcggcggcc
     3541 cgcgcgccca cctgcaggct gtagaattca gtgccttcta tgtcgaggag cggcaggaag
     3601 ctcgcgagcg aggtcgagcg gttgcggtcg ttcgattgct cggcattgcc ggcccaggcg
     3661 aggccgatgc gaagcgcgcg cgagggctca gcgaaacggt ggcgccaacg ctcgagccga
     3721 tcgggcggcg ctttgaggta cccctcaggg g
//
LOCUS       DATNAE010000103         4781 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3413387, whole genome shotgun sequence.
ACCESSION   DATNAE010000103 DATNAE010000000
VERSION     DATNAE010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4781
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3413387"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..898)
                     /gene="fdhD"
                     /locus_tag="VN518_02170"
     CDS             complement(80..898)
                     /gene="fdhD"
                     /locus_tag="VN518_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HXE03410.1"
                     /translation="MTEPVVRVPCSVWRASGHATGERAIPEETAVAFTYNTASYAVMM
                     ATPQDLEDFAVGFSLTEGVVSSADTIESVEIVEEEIGIELRIWLKAREAVEFLGRRRK
                     MAGPTGCGLCGIESLTEAMRPPPKVGEGRTFTPAEIMAAIESLSPMQALNHETRAVHA
                     AGFYEPERGLVALREDVGRHNALDKLAGALARKGTSARAGLVVLTSRISIEMVQKAAM
                     IGAPVLAAVSAPTALAVRMADAAGMTLIAIARKDGFEVFTHPRRVKGKIASHVA"
     gene            1052..1558
                     /locus_tag="VN518_02175"
     CDS             1052..1558
                     /locus_tag="VN518_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03411.1"
                     /translation="MANKASFTPDEWTKVMESVAVTGMAVTAADPSGLWGMLKEALAG
                     GAALAQAKADPHANELVKAVIADFESVDSRHAVQEALKQRFADAKPGDVVPRALEILR
                     QASAVLDAKAPADAPAFKAWLNSVAAKVAEASTEGGFLGFGGVKVSEAEKATLGDIAK
                     ALGTTASA"
     gene            1592..2380
                     /locus_tag="VN518_02180"
     CDS             1592..2380
                     /locus_tag="VN518_02180"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXE03412.1"
                     /translation="MVSLTSLLRFAAIALAIGNAGGFAVAIAAEPKESPCNDDAMIVF
                     DASGSMAGNVGQGIATTIPRIDEARHALAEVLPSATKFRRVGLITYGPGPYQQCNVHL
                     DLKPTANAGAIIMRTVNALTPGGRTPLASAVEQAAEVLDYRNKPGVIVVVTDGEETCG
                     GAPCDLGKKLHAAAKQLTIHVIGYRVKDFSWTGEQSILDVRCLAEQNGGLYIAAESKE
                     DLVEALRKTLDCPMMTQRASPPPSHAYLEIVRLASQGALRRPDH"
     gene            complement(2374..3708)
                     /locus_tag="VN518_02185"
     CDS             complement(2374..3708)
                     /locus_tag="VN518_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014331460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HXE03413.1"
                     /translation="MPTGDGSLANILGILLIFVLVAANGFFVAAEFALVAVRPSRVAQ
                     LVAEGRARARALKRATDNLDAYLAATQLGITISSLALGWIGEPALAHLIEPLLAGLGS
                     FAVAGSHAIAVTIAFTIITALHIVLGELAPKSLALQRSEATALRVVMPLRLFSFLFRP
                     AIVTLNGLGNLVLRLIGLKPAAAEESLHSPEELKLLIAASEQAGLLRHEQLELVSRVL
                     SIGDRPIGDIMTPRVDVDWIDIGDGRDEILKDIRACRHEQLLVGKGGIDAPLGMILKK
                     DLLDQALDGKAIDPMATLREPLVVHEATPIFRVLEQFKKAPVRLAMIFDEYGALEGIV
                     TQTDLLEAIAGDLPSVGEEPDVVERADGSLLINGMMPAEDAFNRFGLRTRPEEGDFHT
                     VAGFVLGQLGHLPEVGEQFEYEGWRFEVVDLDGRRIDKVLATPPKARPDNAQ"
     gene            3913..>4781
                     /locus_tag="VN518_02190"
     CDS             3913..>4781
                     /locus_tag="VN518_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase"
                     /protein_id="HXE03414.1"
                     /translation="MQNSSTRKAAGEREMQKVFENVACPFCGMLCDDLEVENDDGTLK
                     VLKNGCGRAIGGFERKLPASSPQIMGKDVTLAEAVKEAAALIGKADSPLFGGLATGVE
                     GMRAALALAERAGGVVDHALSEGQYRNVRVLQSAGWITSTLTETRNRADLLIIVGSDI
                     RKLQPRFFERIVSPPDSMFEVTAPTRTVVFIGEVPERKDAAGPQVGHVVTLPCKVEQI
                     GEVLGTLRARLRGFRVNAKTAGGIALAKIDALAELCRQAKYGVVVWAPPTLDVPHAEL
                     LVEQATGLVKDLNP"
BASE COUNT          872 a         1534 c         1562 g          813 t
ORIGIN      
        1 cgatcccttc ttcaacatca accggcccga ggatctcgcc aaggcggaag cattgctcaa
       61 ggccgtttga gcgtacgcgc taggccacgt ggctcgcgat cttgcccttc acacggcgcg
      121 gatgagtgaa gacttcgaaa ccatccttgc gtgcgattgc gatcagcgtc atcccggcag
      181 catcggccat gcgcacggca agagccgtcg gcgccgagac cgctgctaga accggcgcgc
      241 cgatcatcgc cgccttttgc accatctcga tcgagatccg gctcgtcagc actactagtc
      301 ccgcgcgcgc gctcgtgccc ttgcgggcga gcgcgccagc aagcttgtcg agcgcattgt
      361 ggcgcccgac gtcctcgcgc aaggcaacca gcccgcgctc gggctcatag aagccggcgg
      421 catgtacggc gcgcgtctca tgattgagcg cttgcatggg tgagagcgac tcgatcgcgg
      481 ccatgatctc ggcgggtgtg aaggtacgac cctcgccgac cttgggcggc ggacgcatcg
      541 cctcggtgag actctcgatt ccgcatagcc cgcagccggt gggtccggcc atctttctgc
      601 gccggccgag aaactcgacg gcctcgcgcg ccttgagcca gatgcggagc tcgatgccga
      661 tctcctcctc gacgatctcc acgctctcga tggtgtcggc cgaggagacc accccttcgg
      721 tcaggctgaa gccgacggcg aagtcctcga gatcctgcgg ggtcgccatc atcaccgcat
      781 aggaggcggt gttgtacgtg aaggcgaccg cagtctcctc ggggatggcg cgttcgcctg
      841 tcgcatggcc gctcgcgcgc cagacagagc aggggacgcg gaccacaggc tcggtcaaat
      901 gcttactccg cttgaattta ttgccgtgga aactctcgcg tgcctgctta caagaagtgc
      961 ggcccgcttc acgtctctgc gacgttcgaa taagcgccag acggcggcgg acaggctaag
     1021 atgatggcgc atacagttaa gggaggcccg catggcgaac aaagcaagct tcacgccgga
     1081 cgagtggacc aaggtgatgg agagcgtggc ggtgaccgga atggcggtga ccgcggcgga
     1141 tccgagcggg ctttggggca tgttgaagga ggcgctggcc ggcggcgccg ccttggcgca
     1201 ggcaaaggcc gatcctcatg ccaacgagtt ggtgaaagcc gtcatcgccg attttgagag
     1261 tgtggacagc cggcatgcgg tgcaggaggc gctgaagcag cgcttcgccg acgcaaagcc
     1321 cggcgacgtt gtccccagag ctctggagat cttgcgccaa gcctctgccg ttctcgacgc
     1381 caaggcgccg gccgatgctc cggccttcaa ggcatggctg aattccgtcg cagccaaggt
     1441 ggcggaggcg tcaacggaag gaggcttcct cggcttcggc ggcgtgaagg tgagcgaggc
     1501 agagaaggcc acccttggcg acatcgccaa agccttgggc acgacggcaa gcgcctgacg
     1561 gcgggatgag gactcacgtg aggtcaagat cgtggtgtcg ttgacctcgc tccttaggtt
     1621 cgcagccatc gccttggcga tcggaaatgc cggcggcttc gccgtggcaa tcgcggcgga
     1681 gccgaaggag tctccctgca acgacgacgc catgatcgtg ttcgatgcgt caggctcgat
     1741 ggccgggaat gtcggacaag gtatcgcgac caccatccca cggattgatg aagcgcggca
     1801 cgcgctggct gaggttttgc cgagcgccac gaaatttcgc cgcgtaggcc tcatcaccta
     1861 tggtccgggt ccttaccagc agtgcaacgt ccacctcgat ctcaagccca ccgccaatgc
     1921 cggggccatc atcatgcgca cggtgaacgc tctcacgccg ggtggccgca cgcccttggc
     1981 ctcggcggtg gagcaggcgg ccgaggtgct ggactaccgg aataagccgg gcgtcatcgt
     2041 cgtggtcacc gacggcgaag agacgtgcgg aggcgcgcct tgcgaccttg gcaaaaaact
     2101 ccatgcggcg gccaagcagc tgaccatcca cgtcatcggc tatcgcgtta aggatttctc
     2161 ttggacaggc gagcaaagca tcctcgacgt ccgctgcctg gccgagcaaa atggtgggct
     2221 ctacatcgcc gcagagtcga aggaagacct ggtcgaagcg ctcaggaaaa ccctcgactg
     2281 cccgatgatg acgcaacgcg cttcgccgcc accatcgcac gcctatctcg agatcgttcg
     2341 gcttgcctca cagggggcgt tgcgccgtcc cgatcattga gcgttgtccg gccttgcctt
     2401 cggcggggtc gccaacacct tgtcgatgcg gcggccgtcc agatcgacga cctcgaaccg
     2461 ccagccttca tactcgaact gctcgccgac ctccggcaag tgcccaagct ggccgagcac
     2521 gaagccggca acagtatgga agtcgccctc ctcgggccgc gtgcgcagac cgaaccggtt
     2581 gaaggcgtcc tcggccggca tcatgccgtt gatgagaagc gagccgtcgg cgcgttcgac
     2641 cacgtcaggc tcttcgccca cgctcggaag atcgccggcg atggcttcga gcagatcggt
     2701 ctgggtgacg atgccttcga gcgcgccata ctcgtcgaag atcatggcaa gccgcaccgg
     2761 cgccttcttg aactgctcaa gcaccctgaa gatcggcgtc gcctcatgca ccacgagagg
     2821 ctcgcggagc gtggccatgg ggtcgatagc cttgccgtcg agggcctggt cgagcaggtc
     2881 cttcttcaag atcattccga gcggcgcatc gatgccgccc ttgcccacga gcagctgctc
     2941 gtggcggcag gcgcggatgt ctttcaggat ctcgtcgcgg ccatccccaa tgtcgatcca
     3001 gtcgacgtcg acgcgcggcg tcatgatgtc gccgatgggg cggtcgccga tgctgagcac
     3061 gcgactgacc agctcaagct gctcatgacg cagaagcccc gcttgctcgc tggcggcaat
     3121 caaaagcttc agctcttccg gcgaatgcag cgattcctcc gccgcggcgg gcttaaggcc
     3181 aatgagccgc agcaccagat tgccgaggcc gttgagggtg acgatcgccg gcctgaacag
     3241 aaacgaaaac aggcgcagcg gcatgaccac gcggagcgcg gtggcttcgc tccgctgaag
     3301 tgccagactt ttcggggcga gctcgccgag cacgatatgc agcgccgtga tgatggtgaa
     3361 ggcgatcgtc accgcgatgg cgtgagaacc ggcgaccgcg aacgagccga ggccggcgag
     3421 cagcggctcg atcaggtggg cgagcgccgg ctcgccgatc cagccgagcg caagcgacga
     3481 aatggtgatg ccgagctggg ttgccgcaag ataggcgtcg agattgtcgg tggccctttt
     3541 cagcgccctt gctctcgcgc gtccctccgc gacaagttgt gccacgcggc tcggccgtac
     3601 cgcgaccagg gcaaattcgg cggcgacaaa aaagccgttg gccgccacca gcacgaaaat
     3661 cagcagaatc ccgagaatgt tggccaaact gccatcgccc gtgggcatgg tctcctctct
     3721 aggttgttca agtctctgcc gcagcataga cgggggcgcg ggcgatgtcg cgccggcctt
     3781 ccgcgcgagc aacgcagctt ggagtcttgg ccaatttgcg ctactgccag gggcgacagg
     3841 tttcgtccct ggagttgagt tgcggtcccg cccgtgcttt cttctcgcgc tttgtgatgg
     3901 ggttctaacg gcatgcagaa ttcgagcacg cgaaaagccg cgggcgagcg cgaaatgcaa
     3961 aaagtgttcg aaaacgtcgc ctgtccgttt tgcggcatgc tgtgcgacga tctcgaggtc
     4021 gagaacgacg acggcacgct gaaggtcctc aagaacggtt gcgggcgggc gatcggcggc
     4081 ttcgaacgca agctgccggc aagcagcccg cagatcatgg gcaaggacgt caccctcgcc
     4141 gaagccgtca aggaagccgc ggcgctcatc ggcaaggccg acagtcccct gtttggcggg
     4201 cttgccaccg gcgtcgaggg catgcgcgcg gcgcttgcgc ttgccgagcg cgccggcggc
     4261 gtggtcgacc atgccttgag cgaagggcaa tatcgcaacg tcagggtgct gcaaagcgcg
     4321 ggatggatca cctccaccct caccgagacg cggaaccgtg ccgatctcct gatcatcgtc
     4381 ggcagcgaca tccgaaagct gcagccacgc ttcttcgagc gcatcgtctc gccgcccgac
     4441 tcgatgttcg aggtcacggc gccgacgcgc accgtcgtgt tcatcggcga ggtgcctgag
     4501 cgcaaggacg cggccggacc gcaggtcggt cacgtggtca cacttccctg caaggtcgag
     4561 cagatcggcg aggttctcgg caccctccgc gcgcgcttac gcggctttcg cgtcaatgcc
     4621 aagaccgccg gcggcatcgc gctcgccaag atcgatgcgc tcgccgagct ttgccgccag
     4681 gcgaaatacg gggtcgtggt gtgggcaccg ccgacgctcg acgtgccgca cgccgagctt
     4741 ctcgtcgagc aggccaccgg gctcgtcaaa gacctcaatc c
//
LOCUS       DATNAE010000104         4199 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2815588, whole genome shotgun sequence.
ACCESSION   DATNAE010000104 DATNAE010000000
VERSION     DATNAE010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4199
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2815588"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="VN518_02195"
     CDS             complement(<1..599)
                     /locus_tag="VN518_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HXE03415.1"
                     /translation="MSVTVRFAPSPTGRLHAGNIRTALLNWMFAQQRGGRLLLRLDDT
                     DRERSTDEFADGIREDLTWLGLAWDREVKQSDRFAIYDAAVEKLKASGRLYPAYETQD
                     ELELKRKRQLARGRPPVYDRAALKLTDADREKLESAGQTPHWRFKLETRDVVWNDLIR
                     GEQHVDAASLSDPVLVRADGSYLYTLPSVVDDIDLGITHV"
     gene            complement(601..2289)
                     /locus_tag="VN518_02200"
     CDS             complement(601..2289)
                     /locus_tag="VN518_02200"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HXE03416.1"
                     /translation="MPNEIKVNPKRDSFRLALAQLNPVVGDINGNLKKARAARAEAAT
                     KGADLIAFTELYLTGYPIEDLVLKPSFQQAAREACEAFARDTSDGGPAVLIGLPWAEG
                     PFVYNAVAYLDRGRIEAVRLKYNLPNYGVFDEKRVFAPGPLPEPIEARGVSLGVPVCE
                     DIWSEEACGTLAKAGALLLIVPNGSPYWMDKQGQRYSIARARIAETELPLAYVNQVGG
                     QDELVFDGASFVLNADGILAVQMPAWEEALAVTEWRQDGKRWRCLPQPLAEIEQGDAA
                     DYLACVTGLRDYVEKNGFPGVVLGLSGGIDSALCVAMAVDALGAKRVHGVMLPYLYTS
                     NESLNDAAQTAEALGIRYDIMPISPAVEGLTKSLASISDGGDSGTAEENLQSRARGTL
                     LMGLSNKFGPMVITTGNKSEVSVGYATLYGDMNGGFNPIKDLYKTQVYALARYRNRER
                     PKSCLGPTGRVIPENVLTKAPTAELKHNQRDQDTLPPYDVLDDILNCLVELELPLTEI
                     VARGHAPDVVKKVERMLYLAEYKRRQAAPGVKITAKNFGRDRRYPITNKFREKL"
     gene            complement(2349..3740)
                     /locus_tag="VN518_02205"
     CDS             complement(2349..3740)
                     /locus_tag="VN518_02205"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HXE03417.1"
                     /translation="MAPRWSPDSWRSKPIEQVPVYPDEEKLAEVEEQLATFPPLVFAG
                     EARELKAKLARVAAGEAFLLQGGDCAESFAEHRANAIRDFFRVLLQMAVVLTYAGGSP
                     VVKLGRIAGQFAKPRSAPTEVQNGKELPSYRGDIINGIEFTEEARTPDPQRMLMAYRQ
                     SAATLNLLRAFAQGGYANLDHVHRWNLGFVKDSPAGHRYQELAEHIAESLRFMRACGL
                     TPERTPELRTTSIYTSHEALMLGYEEAMTRVDSTSGDWYATSGHFLWVGDRTRELDQA
                     HIEFLRGIKNPVGAKIGPACGPDELLRLIDALNPANEPGRLTLIGRFGADKVGKHLPA
                     LIRAVEREGKTVVWACDPMHGNTIKAAGGYKTRPFDLILREVETFFDIHRGEGTHAGG
                     IHVEMTGQNVTECVGGAKAVTETDLSDRYHTHCDPRLNADQSLELAFIVSERLKRERE
                     GRPEPEISIAAGE"
     gene            3865..>4199
                     /locus_tag="VN518_02210"
     CDS             3865..>4199
                     /locus_tag="VN518_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010372436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HXE03418.1"
                     /translation="MDPRHLEHGHRPGEIAKRLKEGPRVSYLRDWVYGGIDGTVTTFA
                     VVAGVVGAALSTKALLILGVANLLADGFSMAAANFSGTRAEMEEYEHVRNMEERHVEI
                     APEGEREEV"
BASE COUNT          716 a         1356 c         1358 g          769 t
ORIGIN      
        1 acatgagtga tgccgagatc gatgtcgtcg acgacgctcg gcagcgtata gagatagctg
       61 ccgtcggcgc gaaccagcac cgggtctgac aacgaggctg cgtccacatg ctgctcgccg
      121 cgtatgagat cgttccacac cacgtcgcgc gtctcgagct tgaagcgcca atggggagtt
      181 tgtccggctg actccaattt ctcgcggtcg gcgtcggtga gcttcagcgc ggcgcggtcg
      241 tagaccggcg ggcgcccacg ggcgagctgg cgcttccgct tcaactcgag ctcgtcctgc
      301 gtctcgtagg ccggatagag ccttccgctc gctttgagct tctccaccgc tgcgtcatag
      361 atcgcgaagc ggtcggactg tttcacctcg cggtcccagg caagaccgag ccaggtcagg
      421 tcttcgcgga tgccgtcggc aaactcgtcg gttgaacgct cgcggtcggt gtcgtcgaga
      481 cgcagcagaa gccgccctcc ccgctgctgc gcgaacatcc agttgagcag cgcggtgcgg
      541 atgttgccgg cgtgcaggcg gccggtgggg ctcggcgcga agcgtacggt gacgctcatg
      601 ctaaagcttc tcgcggaact tgttggtgat gggatagcgc cggtcgcggc cgaaattctt
      661 ggcggtgatc ttcacgccag gcgccgcctg ccggcgttta tactcggcga ggtagagcat
      721 gcgctcgacc ttcttcacca catccggcgc gtgaccgcgc gccacgatct cggtcaacgg
      781 cagctcgagc tcgaccaggc agttgagaat atcgtcgagc acgtcgtaag gaggcagcgt
      841 gtcctggtcg cgctggttat gcttcagctc cgccgtcggc gccttggtca ggacattctc
      901 cgggatgacg cggccggtgg gcccgaggca gctcttcggc cgctcccgat tgcgatagcg
      961 ggcaagggca tagacttgcg tcttgtacaa atccttgatc gggttgaacc cgccattcat
     1021 gtcgccatag agcgtggcat agccgaccga cacctccgac ttgttgccgg tagtgatcac
     1081 catcggaccg aatttgttgg agagccccat aagcagcgtg ccgcgggcgc gcgactgcaa
     1141 gttctcctcg gccgtgcctg agtcgccgcc gtcggagatt gacgcgagac tcttggtgag
     1201 cccctccaca gccggagaga tcggcatgat atcgtagcgg ataccaagcg cctcggcggt
     1261 ctgcgccgca tcgttcaggc tctcattgct ggtgtagaga tagggcaaca tgacgccatg
     1321 cacgcgtttg gcgccaagcg catccaccgc catggccacg caaagtgcgg agtcgatgcc
     1381 gcccgagagc ccaagcacca cgccggggaa gccgttcttc tccacatagt cgcgcaagcc
     1441 agtgacgcaa gcgaggtagt cggcggcgtc gccctgctcg atctcggcga gaggttgagg
     1501 caggcagcgc cagcgctttc cgtcctggcg ccattcggtc acggcgagcg cctcttccca
     1561 cgccggcatt tgcacggcga ggatgccgtc ggcgttgagc acaaaggagg caccgtcgaa
     1621 gacgagctcg tcctggcctc caacctgatt gacataggcc aagggcagtt cagtctcggc
     1681 gatgcgcgcc cgcgcgatcg aatagcgctg gccctgcttg tccatccaat agggcgagcc
     1741 gttcggcacg atgaggagga gagcgcctgc cttggccagc gttccgcagg cctcctcgct
     1801 ccagatgtcc tcacacaccg gcacgccgag cgaaaccccc ctcgcctcga tcggttccgg
     1861 cagcggacct ggggcaaaaa cccgcttctc gtcgaacacc ccgtaattgg gcagattgta
     1921 tttgagcctg accgcctcga tgcggccccg gtccaaatag gccacggcgt tgtacacgaa
     1981 cgggccttcc gcccagggaa ggccaatgag cacggcaggg ccgccgtcgc tcgtatcgcg
     2041 ggcgaaggcc tcgcaggcct cgcgcgccgc ctgctggaac gagggcttca acaccagatc
     2101 ctcgattggg taaccggtga gataaagctc ggtgaaggcg atcaggtcgg cgccctttgt
     2161 cgcagcttcc gcccgcgcag cccgcgcctt cttcaggttg ccgttgatgt cgccaacgac
     2221 ggggttcaat tgcgcgagcg cgagcctgaa gctgtcacgc ttggggttga ccttgatttc
     2281 gttcggcatc agcggaactt agcgacagac gcggacctgt ccagcggcgt ggcgtcgatg
     2341 cgctccacct attctcccgc cgcaatggag atctcaggct caggccgtcc ctcgcgctcg
     2401 cgcttcaacc gctcagaaac gatgaaggca agctcgagcg actggtcggc gttgagccga
     2461 ggatcgcaat gagtgtggta gcggtcggac aaatccgtct cggtcacggc ctttgcgccg
     2521 cccacgcatt cggtgacgtt ctgcccggtc atctcgacat ggatgccgcc ggcatgcgtg
     2581 ccttcgccac gatgaatgtc gaagaaggtc tcgacctcgc gcaggatgag gtcgaagggc
     2641 ctggtcttgt agccgccggc tgccttgatg gtgttgccgt gcatcgggtc gcaggcccac
     2701 accacggtct tgccttcgcg ctccacggcg cggatcagag ccggcaggtg cttgcccacc
     2761 ttgtcggcgc cgaagcggcc gatgagcgta agccgcccgg gctcgttcgc cgggttcagc
     2821 gcatcgatca ggcgcaagag ctcgtcgggc ccgcaagcag gcccgatttt ggcgccgacc
     2881 gggttcttga tgccgcgcag gaactcgata tgcgcctggt cgagttcgcg agtgcggtcg
     2941 ccgacccaga ggaaatggcc tgaggtcgcg taccagtcgc ctgaggtcga gtcgacgcgg
     3001 gtcatcgcct cctcgtagcc gagcatcaac gcctcgtggc tcgtatagat gctcgttgtg
     3061 cgaagctcgg gcgtccgctc aggggtgagc ccgcaagcgc gcatgaagcg caagctctcg
     3121 gcgatatgct cggcgagttc ctgatagcga tgccctgctg ggctgtcctt gacgaagccg
     3181 agattccacc ggtgcacgtg atcgagattg gcgtagccgc cttgcgcgaa cgcgcgcagc
     3241 agattgagcg tcgccgccga ctgccggtag gccatcagca tgcgctgcgg gtcgggcgtg
     3301 cgcgcttcct cggtgaactc gatgccgttg atgatgtcgc cgcgatagct cggcagctcc
     3361 ttgccgttct gcacttcggt cggcgccgag cgcggcttgg cgaattgtcc ggcgatgcgg
     3421 ccgagcttta ccaccggcga gccgccggca taggtcagca ccaccgccat ttgcagcagc
     3481 acccggaaga aatcgcggat ggcgttggcg cgatgctcgg cgaagctttc ggcgcaatcg
     3541 ccaccctgca gcaaaaaggc ctcgccggcg gccacgcggg cgagcttcgc cttcagctcg
     3601 cgcgcctcgc ctgcaaatac cagcggcgga aaggtggcaa gctgttcctc gacctcggca
     3661 agcttctcct cgtccggata gaccggaacc tgctcgatcg gcttagagcg ccagctgtcg
     3721 gggctccatc gcggtgccat ctgaaatctc tccaaaaagg ggctgacgct atataggcgc
     3781 ttcgcccgcg cctggctagc agccagccgt tgtctctgtc tatcgcccgt gccacatagc
     3841 cccctcaagg ccaaggagtc gtaaatggac ccccgacatc tcgagcacgg tcatcgcccg
     3901 ggcgaaatcg ccaagcggct gaaggagggg ccgcgggtca gctatctgcg cgactgggtc
     3961 tatggcggga tcgacggcac cgtgaccacc ttcgccgtgg tcgccggtgt ggtaggcgca
     4021 gcgctttcca ccaaagccct gctcattctc ggcgtggcca atcttctcgc cgacggcttc
     4081 tccatggccg ccgccaattt ctccggcacc agggccgaga tggaagaata cgagcacgtg
     4141 cgcaacatgg aagagcgcca cgtcgagatc gcgccggagg gcgagcgcga ggaggtcag
//
LOCUS       DATNAE010000105         3477 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3714377, whole genome shotgun sequence.
ACCESSION   DATNAE010000105 DATNAE010000000
VERSION     DATNAE010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3714377"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /gene="mce"
                     /locus_tag="VN518_02215"
     CDS             <1..321
                     /gene="mce"
                     /locus_tag="VN518_02215"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HXE03419.1"
                     /translation="KVSPAVDQPEHGVTTVFIELPNTKVELLGVLGDKSPIAAFLARN
                     PNGGIHHLCYEVPDIVAARDRLVAEGARVLGQGDPKIGAHGKPVLFLHPKDFCGTLVE
                     LEQA"
     gene            325..600
                     /locus_tag="VN518_02220"
     CDS             325..600
                     /locus_tag="VN518_02220"
                     /inference="COORDINATES: protein motif:HMM:NF018978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1467 family protein"
                     /protein_id="HXE03420.1"
                     /translation="MALVFAISMYVVIWWIVLFAMLPIGVRTQAEEGEVSPGTPESAP
                     HQPRLLPKMLATTVVASLVFAAVYAIVVHRVITLDQIPFLPRYETVR"
     gene            complement(688..963)
                     /locus_tag="VN518_02225"
     CDS             complement(688..963)
                     /locus_tag="VN518_02225"
                     /inference="COORDINATES: protein motif:HMM:NF018978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1467 family protein"
                     /protein_id="HXE03421.1"
                     /translation="MTLSFAIAIYAIIWWIVLFAVLPIGVRTQEEEGEVSPGTAESAP
                     HMPNLLPKMLATTIVASVVFAVVYVIIVHRVITLDQIPFLPRYESVR"
     gene            complement(967..1371)
                     /gene="mce"
                     /locus_tag="VN518_02230"
     CDS             complement(967..1371)
                     /gene="mce"
                     /locus_tag="VN518_02230"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006934701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HXE03422.1"
                     /translation="MIGRLNHVAIAVPDLAAATKVYRDTLGAKVSPAVDQPEHGVTTV
                     FIELPNTKVELLGALGDKSPIAAFLARNPNGGIHHLCYEVPDIVAARDRLVADGARVL
                     GQGDPMIGAHGKPVLFLHPKDFCGTLVELEQA"
     gene            complement(1485..3170)
                     /locus_tag="VN518_02235"
     CDS             complement(1485..3170)
                     /locus_tag="VN518_02235"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HXE03423.1"
                     /translation="MSRRPDRQRPEFVFVPLGGVGEIGMNLYLYGYGPPDDREWLIVD
                     MGITFGGETEPGVDVILPDIGFLEAERQSLKALLLTHAHEDHLGAVADLWPRLGGVPV
                     YATPFTAAMLKSKLGEAELLPDFPLHVVPLAHRFTIGPFDIELVNMSHSIPEPSAVII
                     KTDLGTVFHTADWKLDEHPLTSAPTDVARLKALGEEGVTALICDSTNAIRDGMSPSEA
                     DVAVTLERLIAEAPRRVAVTIFASNVARIRSVANAARAAGRRLVVVGRAMYRVIEAAQ
                     ETGYLDPDLAFHEESEFENIPARKVVALCTGSQGEARAAMARIALDEHPNVSLDAGDR
                     VIFSSRTIPGNEKAVGRVQNGLADLGIEVITDGDAPVHVSGHPRRGELEQLYRWVRPQ
                     IAIPMHGEGRHLEAQAGLAETLGVPEVIRARNGAMVRLAPPPAAIIDDVPVGRLYRDG
                     AIITCADDGQVRERRKLSFAGSVSVSLVLSDKGMLLADPEVALSGIPATDMQGTSFET
                     IAREAAIGTIESIPRPRRKDQALVSEAVRRGVRAAVNQAWGKKPVCSVLLTVI"
     gene            complement(3197..>3477)
                     /locus_tag="VN518_02240"
     CDS             complement(3197..>3477)
                     /locus_tag="VN518_02240"
                     /inference="COORDINATES: protein motif:HMM:NF014310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HXE03424.1"
                     /translation="GGSATPAEAFETLAAVTDAWLSRWAEGVAFQTVRRAWLDRAGPT
                     GRPLRVRLPGEEAEGTYAGLDSGGALRLLMSDGAERRIAAGDVFFATS"
BASE COUNT          616 a         1131 c         1148 g          582 t
ORIGIN      
        1 aaagtctcgc cagcggtcga tcagcctgag cacggcgtca ccacggtgtt catcgagctt
       61 cccaacacca aggtcgagct gctcggcgtg ctcggcgaca aatccccgat cgctgccttc
      121 ctggcgcgta accccaatgg cggcatccac catctctgct acgaggtgcc agacatcgtc
      181 gcggcgcgcg accggctcgt cgccgaaggc gcccgcgtgc tcggtcaagg cgatcccaag
      241 atcggcgccc atggcaagcc cgtgctcttc ctccatccca aggatttctg cggcacgctg
      301 gtggagctcg agcaggcgta agccatggcg ctcgtattcg ccatctccat gtacgtggtg
      361 atctggtgga tcgtgctgtt cgccatgctg ccgatcggcg tgcgcaccca ggccgaggaa
      421 ggcgaggttt ctcccggcac gccggaaagc gcgccgcacc agccgagact gctgccgaag
      481 atgctggcca cgacggtggt cgcgagccta gtgttcgcgg cggtctatgc gatcgtcgtc
      541 caccgcgtga tcacgctcga ccagattccc ttcctgccgc gctatgaaac ggtgcgctga
      601 ccgctggcct ccgaggcgtc cgcacaaaaa aagcaaggtc taacggcctt gctttttttg
      661 ttgtgccaat gccacccgcg ggagcggtta gcgcacagat tcgtagcgcg gcaggaaggg
      721 aatctggtcg agcgtgatca cgcgatggac gatgatcaca tagaccaccg cgaacaccac
      781 gctcgcgaca atcgtggtgg cgagcatctt cggcagcaaa ttcggcatat gcggcgcgct
      841 ttcggccgta ccgggcgaga cctcgccctc ctcctcctgc gtgcgcacgc cgatcggcag
      901 tacagcaaac agtacgatcc accagattat ggcgtagatg gcgatggcga agctgagcgt
      961 catggcctag gcctgctcga gctccaccag cgtgccgcaa aagtccttgg gatggaggaa
     1021 gagcaccggc ttgccgtggg cgccgatcat tgggtcgccc tggccgagca cgcgggcgcc
     1081 gtcggcgacc agccggtcgc gcgcggcgac gatatcgggc acctcgtagc agagatggtg
     1141 gatgccgcca ttgggattgc gcgccaggaa ggcagcgatc ggggatttgt cgccgagcgc
     1201 gccgagcaac tcgaccttgg tgttgggcag ctcgatgaac accgtggtga cgccatgctc
     1261 aggctgatcg accgccggcg agactttggc gccaagcgtg tcgcgataca ccttggtcgc
     1321 tgccgcaaga tcgggaacgg caatggcaac gtggttcaaa cgtccgatca tctgctttct
     1381 cgctccaatc gtcatgtccg ggactgcgcg cggtcatgag aagccattag catggtcttg
     1441 agcaagttat ctaatgcgct atcgcgcttc gcgctacttg agcgctagat caccgtcagc
     1501 aacacggagc ataccggctt cttgccccag gcctgattga cggcggcgcg cacgccgcgg
     1561 cggacagctt cgctgacgag cgcctgatct ttgcgccgcg gccgcgggat gctctcgata
     1621 gtgccgatgg cggcctcgcg cgcgatggtc tcgaacgagg tgccttgcat gtccgtggcg
     1681 ggaatgccgg agagcgccac ctccggatcg gcgagcagca tccccttgtc cgacagcacc
     1741 agcgacaccg acacggagcc ggcaaagctc agcttgcgcc gctcgcgcac ctgcccgtca
     1801 tcggcacaag tgatgatggc accgtcgcga taaaggcgcc ccacaggcac gtcgtcgatg
     1861 attgccgcgg gcggtggcgc caagcgcacc atcgccccgt ttcgcgcgcg gatcacctcg
     1921 gggacgccga gcgtctccgc gagccccgcc tgagcctcaa gatgcctgcc ctcgccgtgc
     1981 atcgggatgg cgatctgcgg acgcacccag cggtagagct gctcgagctc gccgcgccgc
     2041 ggatgccccg acacatgcac cggggcgtcg ccgtcggtga tcacctcgat gccgagatcg
     2101 gcgaggccgt tctgcacgcg cccgaccgcc ttctcattgc ccgggatggt gcgggaggag
     2161 aagatcaccc gatcgccggc gtcgaggctg acattggggt gctcatccaa agcgatccga
     2221 gccatggcgg cgcgggcctc cccctggctg ccggtgcaga gcgcaacgac cttccgcgcc
     2281 gggatgttct cgaattcgct ctcctcgtga aaggcaaggt caggatcgag atagccggtc
     2341 tcttgcgcgg cttcgatcac gcggtacatg gcgcggccga ccaccacgag cctgcggccg
     2401 gcggcgcgcg cggcattggc aacggagcgg atgcgggcga cgttcgaggc gaaaatggtc
     2461 accgccacgc gtcgcggcgc ctcggcgatc aggcgctcga gcgtgaccgc gacatcggcc
     2521 tccgacggcg acatgccgtc acggatggcg ttggtcgagt cgcagatgag cgcggtgacg
     2581 ccttcttctc ccagcgcctt gaggcgcgcc acgtcggtcg gcgcgctggt caacgggtgc
     2641 tcgtcgagct tccagtcggc ggtatggaac accgtgccga gatcggtctt gatgatcacc
     2701 gccgaaggct cggggatcga atgggacatg ttgacgagct cgatgtcgaa cggaccgatg
     2761 gtgaagcggt gtgccaatgg aacgacatga agcggaaaat ctgggagaag ctcggcctcg
     2821 ccgagtttcg acttcagcat cgcggcggtg aagggcgtcg catagacggg cacgccgcca
     2881 agccttggcc aaagatcggc cacggcgcca agatgatcct catgggcatg ggtgaggagc
     2941 agggccttca agctctggcg ctcagcctcg aggaaaccga tatcgggtag gatcacgtcg
     3001 acgccgggct ccgtctcgcc gccgaaggtg attcccatgt cgacgatgag ccactcgcgg
     3061 tcgtccggag gcccgtagcc gtagagatag aggttcatgc cgatctcccc caccccgcca
     3121 agggggacga acacgaactc tggcctttgt cggtcgggac gtcgggacat gctctagact
     3181 aaggctaggg gattgatcac gaggtggcga aaaacacgtc gccggcggcg atgcggcgct
     3241 cggcaccatc gctcatcagg agcctgagcg caccccctga atcgaggccg gcataggttc
     3301 cttccgcctc ttcgccgggg aggcgcacgc gcaaaggacg gccggttggc cctgcccggt
     3361 cgagccaggc gcgccgcacc gtttggaacg cgaccccctc cgcccagcgg gagagccagg
     3421 catcggtgac ggcggcaagc gtctcgaagg cttcggccgg ggtcgccgaa ccgccat
//
LOCUS       DATNAE010000106         9238 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2816716, whole genome shotgun sequence.
ACCESSION   DATNAE010000106 DATNAE010000000
VERSION     DATNAE010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 9238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9238
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2816716"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1046..3064)
                     /locus_tag="VN518_02245"
     CDS             complement(1046..3064)
                     /locus_tag="VN518_02245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HXE03425.1"
                     /translation="MAARRKPKPAAKAAKGKERVEPHFGGKRTPARKPRKTARRSASA
                     SPIRRALLRGLFTTLALIALIIVGSFLYFVARLPDPILLTLDDRPPNLTILASDGTVL
                     AERGLRRGYVRLDRLPPYLPQAVIATEDRRFYNHLGVDPVGLVRAGMRNLIAGSVVQG
                     GSTITQQLAKNLFLSPDRTMARKLEEAMYAIWLERRFTKDEILELYLNRVYFGGGTYG
                     VEAAARRYFGRSARSVTLTQAALLAGLLKAPSRYAPTRSVELATTRVDVVLDNMVEAG
                     FLSSAEAETAAAEPLRLRTFSDETGYPYAVDWVAETLPEVVGNTEGDLVVETTIDAGL
                     QRAAQLTLRQTLDAEGKDLDVSEGAVVVLDTSGGIKALIGGRSYRSSPFDRAVKALRQ
                     PGSAFKPFVYLTALESGYTPDSIANDEPVAVDGWAPKNHTGTYRGAVTLRDSFAQSIN
                     TVAVKLADEVGRGNVVSTARRLGIRSELHDRPSLALGTAEVTPLELVAAYVPFANGGE
                     GITPHIIARVRNGDGKLLYEYKGAGLTRVVDESYVAEMNDMMKATMVSGTGRQAALPD
                     QIAGGKTGTSQNSRDAWFVGYTAHYVGGVWVGNDDGSKMRNVTGGTLPAHIWHDIMVY
                     AHEGKQPLALPGTRSPWLEEASSRLWTAPTEKSNAPLYRRMIDLLSGG"
     gene            complement(3172..5604)
                     /gene="gyrB"
                     /locus_tag="VN518_02250"
     CDS             complement(3172..5604)
                     /gene="gyrB"
                     /locus_tag="VN518_02250"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HXE03426.1"
                     /translation="MKRASERKAAASSDYGAESIKVLKGLDAVRKRPGMYIGDTDDGS
                     GLHHMVYEVVDNAIDEALAGYCDEIQVTLNADGSVTVVDNGRGIPTEIHKGEGVSAAE
                     VIMTQLHAGGKFDQNSYKVSGGLHGVGVSVVNALSSRLDLTIWRDGKEHFMRFAHGVP
                     EVPLAEVGPATRTGTRVTFVPSTRTFTMVEFDYATLEHRLRELAFLNSGVRIVLSDHR
                     GVEPKTEEMRYEGGLRAFVRFLDRTKHPLIPEPIVVEKEQDKIVVEAALWWNDSYHEN
                     VLCFTNNIPQRDGGTHLAGLRSALTRTVNTYAAESGIAKKEKVALTGDDAREGLTCVL
                     SVKVPDPKFSSQTKDKLVSSEVRPVVEGVMAAALSQWFEENPGEARAVVQKIVEAAAA
                     REAARKARELTRRKGALDISSLPGKLADCQERDPSKSELFLVEGDSAGGSAKQARDRA
                     FQAVLPLRGKILNVERARFDKMLSSQEIGTLITALGTGIGRDDFDLAKLRYHKVIIMT
                     DADVDGAHIRTLLLTFFYRQMPQLVEAGNLFIAQPPLYKVKRGSQERYLKDDKSLEDY
                     LVSEGLEDTVLLTGDKAVRAGADLRDIVDTARKITGILKGLHSRYPRFIIEQAAIAGA
                     LAPKLLKDAKQAQGAAAYIGKRLDALAEETERGWHGEALPEGGLRFWRELRGVMESHL
                     VDGPFIASADALKLDQYAEHLQEVYAKPATLKRKDISTAVHGPSDLLDAVMAAGRKGL
                     TLQRYKGLGEMNPEQLWETTLDLESRTILQVKVGELDEADEIFSRLMGDVVEPRREFI
                     QANALQVANLDV"
     gene            complement(5673..6830)
                     /gene="recF"
                     /locus_tag="VN518_02255"
     CDS             complement(5673..6830)
                     /gene="recF"
                     /locus_tag="VN518_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HXE03427.1"
                     /translation="MGEAAWAESRLFVQELRLTNFRNYERAAASLDASLVVLVGDNGA
                     GKTNLLEAVSLLGAGQGLRGRPYEELTRKDGLGGWAVAATVMSLEGVVEIGTGFGLSG
                     ASEGTGRNVRIAGKDQSAGSLAHYVQLVWVIPEMDGLFTGPASERRRFLDRLMLAIDP
                     KMSGPRGRYERAMRQRNRLFQMREGSPSLYAGLEEQMAEAGVAIAAARLDAIARLSDL
                     IQVTHEARGPGPFPFAVLSLEGTLERALMERPAVEVEDDYVSLLSEARERDRLAGRAL
                     AGPHLSDLIVFHGPHNAPAGDCSSGEQKALLLGLVLAKARLIRELSGAAPLVLLDEVA
                     AHLDHARREALFGEILDLKAQAWMTGTDRETFASLASQAQILVVNRGRIGA"
     gene            complement(6875..7993)
                     /gene="dnaN"
                     /locus_tag="VN518_02260"
     CDS             complement(6875..7993)
                     /gene="dnaN"
                     /locus_tag="VN518_02260"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HXE03428.1"
                     /translation="MQFTIDKPALTKALAHVASVVERRNTIPILSNVHIATRNNELKL
                     TSTDLDMEIVETVPARAKGEGATTVPAHMFHDIVRKLPEGTDIDIAREGDQGRLTISC
                     GQARFSLQTLPADDFPSLSVDDLGHAFTMSAADLKRLIEKTRFAISTEETRYYLNGIY
                     LHAAESGGKKVLRAVATDGHRLAQVELPLPAGAKDMPGVIVPRKTVAELAKLAEDGDG
                     DVRVELSPSKIRVSTARVVLTSKLIDGTFPDYERVIPQGNDKTMEVDNHAFAEAVDRV
                     STLSSDKGRAVKLSITDGKLTLSVNNPDSGSASEEIPVEYGFDPLEIGFNARYLLDIS
                     GQLEGGTAEFQLADPGSPTMIRDGKDASALYVLMPMRV"
     gene            complement(8187..>9238)
                     /gene="dnaA"
                     /locus_tag="VN518_02265"
     CDS             complement(8187..>9238)
                     /gene="dnaA"
                     /locus_tag="VN518_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013417700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HXE03429.1"
                     /translation="FEGSPLDPRLTFATFLVGTSNRLAHAAAQEVAGAFSTPQQLFNP
                     LFVHGSVGLGKTHLLHAISWDVKQRKPEAQVLYLTAERFMSGFVQALRARDALAFKEK
                     LRKIDILLIDDMEFLQGPTIQQEFCHTLNSLIDGGRQVVVAADRAPTQLDKLDMRMRS
                     RLGGGLVAEIGIMDYELRHKILSKRAQEACSETRGLAISDTVLAFLAERLTESGRELE
                     GAVHRLRASYQLTDEQVTIEIAEQIVRDLMRGAEPRRVRIDDILRTVSKHYGVNRGDL
                     LSGRRNRSIVRPRQIGMYLAKLLTSRSLPEIGRRFGNRDHTTVLHAIRKIEQLMNDDN
                     QLREEIELLKRLLRD"
BASE COUNT         1548 a         2921 c         3053 g         1716 t
ORIGIN      
        1 cttcggacga ttctgcgtac gtcgtcgggg cagacttcct gatcgacggc gtggtctcag
       61 ccatttcgcc cgtgggcgct tgagcttcgt tgcagagagt cggggccgtt cctgttccgc
      121 tcaggcaagg gacggctcgc accaccacgg gctgccggta cgcaggcgtg atgtccggta
      181 tgggtcagta gcgcacgtcc agcttggcga acagaatgtc cgctaagtgc caataaccga
      241 caacagtcgc tatcaaacca agctcggtca atgcgatccg ttcgcttcgc gagataagcc
      301 agagaatcca cagctaggtt ttggagcctg cgcttcgcgc gtgttggccc gcttcacctg
      361 ggcgccctta tttaccgggg gacagagacg ttcccgcctg caccagagca atgttaacct
      421 agtcgccgcc ggagagagag tcgtgagcgt ccggggcctt ctgcgtcaac atgatgcgcg
      481 gtagccagga actgacttcc ctcgtcagtc aatcgattga actagcgacc caccgagctg
      541 tttccagctc atttcctata accgagcttc caagaaccaa ttcaatgagg cggtcgtccg
      601 gccggtttgc ttctgcttag gccgtcaaac aatgtccgct ttgggtcatt ctgagaagtc
      661 agcgctcaag ccagaaaggt ccgctatccg actatgtgcg gacatttgaa gtcacaatgg
      721 gacgcggatg tttggtgttt caagggctcc gaaaaggcct aaaggattat gtcgaggacc
      781 gggctcgacc tcgtacttat tgtgctaccc aaatgctggc tgcatcaagc gcgccctcca
      841 tatttttgat taatacattg tgcaataact gtttttttgc agcttccgta ggcttctcac
      901 tccccagcgg ggagagggtt ggggtgaggg ggtcggacgc ttgtcgtcgg gctctgtgtc
      961 cccctcaccc gcaccttcgc caaagcgcga aggtgcgacc tctcccccaa ggggagaggt
     1021 taaaggcctt cggcctcgaa tgagcttagc cgccggagag aaggtcgatc atgcgccggt
     1081 agagcggtgc gttgctcttc tcagttggcg cagtccacag ccgcgacgat gcctcctcga
     1141 gccaaggcga gcgcgtgcca ggcagcgcca aaggctgctt gccctcatgc gcgtagacca
     1201 tgatgtcgtg ccagatatgc gcgggcagcg tgccgccggt cacgttgcgc atcttcgagc
     1261 cgtcgtcatt gccgacccag acgccaccga cataatgcgc ggtatagccg acgaaccagg
     1321 cgtcgcgtga gttttggctg gtgcccgtct tgccgccggc gatctggtct ggcagggccg
     1381 cctgccttcc ggtgccgctg accatggtcg ccttcatcat gtcgttcatt tccgcgacat
     1441 aggactcatc gacgacgcgg gtaagccccg ctcccttgta ctcgtaaagc agcttgccgt
     1501 cgccgttgcg cacgcgggcg atgatatgcg gggtgatccc ttcgccgcca ttggcgaacg
     1561 gcacgtaggc ggcgacgagc tccaaggggg tgacctcggc ggtgccgagc gcgagcgacg
     1621 ggcggtcgtg cagctcagag cggatgccga gccgccgcgc cgtgctgacc acgttgccgc
     1681 ggccgacctc atcggcaagc ttcaccgcga cggtgttgat cgactgggcg aagctatcgc
     1741 ggagcgtgac cgcgccgcga taggtgccgg tgtggttctt gggcgcccag ccgtcaacgg
     1801 cgaccggctc gtcattggcg atcgagtcgg gcgtgtagcc gctttcgagc gcggtgagat
     1861 agacgaaggg cttgaaggcc gagccgggtt ggcggagcgc cttcacggca cggtcgaagg
     1921 ggcttgagcg gtaggagcgg ccgccgatca gcgccttgat cccgccgctc gtatcgagca
     1981 ccaccacggc gccttcgctc acgtcgaggt cctttccttc cgcatcgagc gtctggcgca
     2041 gcgtcaattg cgcggcgcgc tgaaggccgg catcgatggt ggtctcgacc accagatcgc
     2101 cctcggtgtt gccgaccacc tcaggcaagg tctccgccac ccagtcgacg gcataaggat
     2161 atcccgtctc gtcgctgaag gtgcggagcc gcaaaggttc tgcggcggcg gtctcagctt
     2221 ccgcgcttga gaggaacccg gcctcgacca tattgtcgag caccacgtcg acgcgcgtgg
     2281 tggcgagctc gacactgcgc gtcggcgcat agcgcgacgg agccttgagc aggccggcaa
     2341 gcagcgccgc ttgcgtcaag gtcaccgacc gagccgagcg cccgaaatag cgccgcgccg
     2401 cggcctcaac gccataggtg ccgccgccga aatagacgcg gttgaggtaa agctcgagaa
     2461 tctcgtcctt ggtgaagcgc cgctcgagcc agatggcgta catcgcctct tcgagcttgc
     2521 gcgccatggt gcggtccggc gacaggaaca ggttcttggc gagctgctgg gtgatggtcg
     2581 agccgccctg caccacgctg ccggcaatga gattgcgcat gccggcgcgg acgaggccga
     2641 ccgggtcgac gccgagatgg ttgtagaagc ggcggtcctc ggtggcgatc accgcctgcg
     2701 gcaggtaagg cggcagccgg tcgaggcgga catagccgcg cctcaagccc cgctcggcga
     2761 gcacggtgcc gtcggaggcg aggatggtga ggttcggcgg ccggtcgtcg agggtaagca
     2821 ggatcgggtc ggggaggcgg gcgacgaaat agaggaagct gccgacgatg atgagcgcga
     2881 tcaaggccag cgtggtgaag agaccgcgga gcagggcgcg gcgaattggg ctggcgcttg
     2941 cgctgcggcg ggcggtcttg cggggtttcc gtgcaggcgt gcgctttccg ccgaaatgcg
     3001 gttcgacgcg ctctttcccc tttgccgcct tcgccgctgg ctttggcttc cgccgcgctg
     3061 ccatggattg cctgccccaa acgccagcga tcgcggggga ggcgccccca cgaaccttgg
     3121 cgcccagagg ctaagcgctt cgcattgacc cagggttaag ggattggtgg ctcaaacatc
     3181 cagattggcg acctggagcg cgttggcctg aatgaattcg cggcgcggct cgaccacgtc
     3241 gcccataagc cgcgagaaga tctcgtcggc ctcgtcgagc tctccgacct tcacttgcaa
     3301 gattgtgcgg ctttcgaggt cgagcgtcgt ctcccacagc tgctctgggt tcatttcgcc
     3361 cagacccttg tagcgctgga gcgtaagccc cttgcggccg gccgccatca ccgcgtccag
     3421 caggtccgaa ggcccgtgca ccgcggtgga aatatccttg cgcttgagcg tcgctggctt
     3481 ggcgtagacc tcctgaagat gctcggcata ttggtcgagc ttgagagcat cggcggacgc
     3541 gatgaacggc ccatccacaa ggtgtgattc catcacgccg cgaagctcac gccagaagcg
     3601 caagccgcct tcgggcagcg cctcgccatg ccagccgcgc tcggtctcct cggcgagcgc
     3661 atcgaggcgc ttgccgatat aggcggctgc gccttgtgcc tgtttcgcat ctttcagcag
     3721 cttcggcgcg agtgcgccgg cgattgctgc ctgctcgatg atgaagcgcg gatagcgcga
     3781 atgcaggccc ttgaggatgc cggtaatctt gcgcgcggtg tccacgatgt cgcgcaaatc
     3841 ggcgccggcg cggacggctt tgtcgccggt gaggagcacg gtgtcttcga ggccctcgga
     3901 aacgagatag tcttcaagcg acttgtcgtc cttgaggtag cgctcttggc tgccgcgctt
     3961 caccttgtaa agcggcggct gggcgatgaa gaggttgccg gcttccacga gctgcggcat
     4021 ctgccggtag aagaaggtga gcagcagcgt gcggatatgg gcgccgtcga cgtcggcgtc
     4081 ggtcatgatg atgaccttgt ggtagcggag cttggcgaga tcgaaatcgt cgcgcccgat
     4141 gccggtgccg agcgcggtga tcagcgtgcc gatctcttgg ctggacagca tcttgtcgaa
     4201 gcgcgcccgc tccacattga ggatcttgcc acgcaacggc agcaccgcct gaaaggcgcg
     4261 gtcgcgcgcc tgcttggctg agcctccggc actgtcgccc tcgaccagga acagctcgga
     4321 cttggatgga tcgcgctcct ggcagtcggc gagcttgccg ggaagcgacg agatgtcgag
     4381 cgcgcccttg cgccgcgtca gctcgcgggc tttccgcgca gcctcgcgcg cggcagcagc
     4441 ctcgacgatc ttctgcacca cggcgcgcgc ctctcccgga ttctcctcga accactgcga
     4501 aagagctgcg gccatgacgc cttcgaccac cgggcgcact tcggaggaga cgagcttatc
     4561 cttcgtttgg ctggagaatt tcggatcagg caccttgacc gagagcacgc aagtgagccc
     4621 ctcgcgcgcg tcgtcgccgg tcaacgccac cttctccttc ttggcgatgc ctgattcggc
     4681 tgcgtaggtg ttgacggtgc gggtgagcgc cgagcgcaag ccggcgagat gcgtgccgcc
     4741 gtcgcgctgc gggatgttgt tggtgaagca gagcacgttc tcgtggtagc tgtcgttcca
     4801 ccacagcgcc gcctcgacca cgatcttgtc ctgctccttc tccaccacga tgggctcagg
     4861 gatcagcgga tgcttggtgc gatcgaggaa gcgcacgaag gcgcgcaagc ctccctcata
     4921 gcgcatctcc tcggtcttcg gctcgacgcc acgatggtct gaaagcacga tgcgcacgcc
     4981 ggaattcagg aacgccagct cgcgcaagcg atgctcgagc gtcgcgtagt cgaactcgac
     5041 catggtgaag gtcctagtcg aaggcacgaa ggtgacgcgc gtgccggttc tggtcgcggg
     5101 gccgacctcg gcgagcggca cctcgggcac gccgtgggca aagcgcatga aatgctcctt
     5161 gccgtcgcgc cagatggtga ggtcgagcct cgacgacaac gcattcacca ccgagacgcc
     5221 cacaccatgc aggccgccgg acaccttgta ggagttctgg tcgaacttcc cgccggcgtg
     5281 gagctgggtc atgatcactt cagccgccga gacgccttcg cccttgtgga tctcggtcgg
     5341 gatgccgcgg ccgttgtcca ccaccgtgac cgagccgtcg gcgttgagcg tcacttggat
     5401 ctcgtcgcaa tagccggcca aagcctcgtc gatggcattg tccaccacct cgtagaccat
     5461 gtggtgcagg cccgagccgt cgtcggtgtc gccgatatac atgccgggcc gcttgcgcac
     5521 ggcatcgagg cctttcagca ccttgatgga ctcggcgccg tagtctgagg acgcggctgc
     5581 ttttctctct gaggctcgtt tcattgggtc cttaggggtc aatttttctg tctttttttg
     5641 cacgtctcgg ccggtggaaa ccggtgccct gatcaggcgc ctattctacc ccgattcact
     5701 accaagattt gggcctgcga cgccaaagag gcgaaggttt cccggtcggt tccggtcatc
     5761 caggcctggg ctttcaggtc cagaatctcg ccaaaaaggg cctcccggcg ggcgtgatcg
     5821 agatgggcgg caacctcgtc gaggaggacg aggggagcag cgccggaaag ctcgcggatc
     5881 agcctcgcct tggcgagcac gaggccgagc agaagcgcct tctgctcgcc cgaggagcag
     5941 tcgcctgcgg gggcattgtg ggggccgtga aacactatta ggtcgctcag atgcggtcct
     6001 gcaagggccc ggccggcgag gcggtcgcgc tcccgcgctt ccgataacag gctcacatag
     6061 tcgtcctcga cctcgaccgc cgggcgctcc atgagggcgc gctcgagcgt gccttcgagg
     6121 ctgagcacgg cgaaggggaa gggccctggc ccgcgcgcct catgcgtcac ctggatcaga
     6181 tcagagagcc gcgcgatcgc atcgagcctt gccgcggcga tggccacgcc ggcttcggcc
     6241 atctgctcct caagcccggc atagagcgaa ggcgatccct cgcgcatctg gaacaggcgg
     6301 ttccgctgcc gcatggcacg ctcgtagcgt cctcgcggcc cgctcatctt cgggtcgatg
     6361 gcaagcatca gccggtcgag gaagcggcgc cgctcggaag ctggaccggt gaacagccca
     6421 tccatctcgg ggatcaccca gacgagctgc acgtaatggg caagcgagcc ggcgctctgg
     6481 tccttgccgg cgatgcgcac gttgcggcct gtgccttcgc ttgcgccgga aagtccgaag
     6541 ccggtgccga tctcgaccac gccttcgagc gacatcaccg tagcggccac cgcccagccg
     6601 ccgaggccgt ctttgcgggt gagttcctca tagggacgcc cgcgcaagcc ctgaccggca
     6661 ccgagcagcg acaccgcctc gagcagattg gtcttgcccg cgccgttgtc gccgacgagc
     6721 accacgaggc tcgcatcgag gctcgccgcc gcccgctcgt aattccggaa attggtcagc
     6781 ctcagctctt gcacaaagag ccgtgactcg gcccaagcgg cttcccccat cgcctcacgt
     6841 gcagcccgct cgcgatatcc gctcagggcc gccctcatac ccgcatcggc atgagcacat
     6901 agagagcgga tgcatccttg ccgtcgcgga tcatggtggg agagcccgga tcggcgagct
     6961 ggaactcggc ggtgccgcct tcgagctgcc ccgagatgtc gagcaggtag cgggcgttaa
     7021 atccgatctc gagcggatcg aagccatact cgacggggat ctcttcgctc gcactgccgc
     7081 tgtccggatt gttgaccgaa agggtgagct tgccgtcggt gatggagagc ttcaccgcgc
     7141 ggcccttgtc gctcgacagc gtggagacgc ggtccacggc ctcggcgaag gcgtggttgt
     7201 cgacctccat ggtcttgtcg ttgccttgcg ggatcacgcg ctcatagtcc gggaaggtgc
     7261 catcgatcag cttggaggtg agcaccacgc gcgcggtcga gacgcggatc ttcgacggcg
     7321 acagctcgac gcgcacgtcc ccgtcgccgt cctcggcgag cttggcaagc tcggcaacgg
     7381 tcttgcgcgg cacgatcacg ccgggcatgt ccttggcccc ggcgggcaac ggcagctcga
     7441 cctgcgcgag gcgatggccg tcggtggcga cggcccggag caccttcttg ccgccgcttt
     7501 ccgcggcgtg gagatagatg ccgttcaggt aatagcgcgt ctcctcggtg gagatggcga
     7561 agcgggtctt ctcgatgagg cgcttgagat ccgccgcact catggtgaag gcgtggccga
     7621 gatcgtcgac gctaaggctc ggaaagtcgt cggccggcag cgtctggagc gagaagcgcg
     7681 cttgaccgca actgatggtg agccgtccct gatcgccctc tcgcgcaatg tcgatgtcgg
     7741 ttccctcggg cagcttgcgc acgatgtcgt ggaacatatg ggctggcact gtggtggcgc
     7801 cttcgccctt ggcccgcgcc ggcaccgtct cgacgatctc catatcgagg tcggtcgagg
     7861 tcagcttcag ctcgttgttg cgcgtggcga tatggacgtt ggacaggatg ggaatggtgt
     7921 tgcgccgctc gaccacgctc gccacatggg cgagcgcctt ggtcagagca ggcttgtcga
     7981 tggtgaattg catgggttaa gcgagaccac tccggcgccc cttcgggatg gttgcatccg
     8041 gccgaagccg tcaaggaccc ggcgagaacc ccgaaaaggc gcgatgaaag ctaaagactc
     8101 ccgcttcaac ttgacccgat aggggcaaat ttgcaagagc gggaggaggg gccggaaggc
     8161 cccatttcct cggaatttca acccccctag tcgcgtagca gccgcttcag aagctcgatc
     8221 tcctcgcgca gctgattgtc gtcgttcatt agctgctcga tcttgcggat ggcgtgcagc
     8281 accgtggtgt gatcgcggtt gccgaacctg cggccgatct ccggcagcga gcgcgaggtc
     8341 aagagcttcg cgaggtacat gccaatctgg cgcgggcgca cgatggaccg gttgcgccgc
     8401 cccgacagca ggtcgccgcg gttcacgccg tagtgcttcg ataccgtgcg caggatgtcg
     8461 tcgatccgca cccgtcgcgg ctccgcgccc cgcatcagat cgcgcacgat ctgctcggcg
     8521 atctcgatgg tcacctgctc gtcggtgagc tgatagcttg cgcgcaagcg gtgaacggcg
     8581 ccttcaagct cgcgcccgct ctcggtcagg cgctcggcga ggaaggcgag gacggtgtcg
     8641 gagatggcga gcccgcgcgt ctcggaacac gcctcttgcg cgcgcttcga caggatcttg
     8701 tggcggagct cgtagtccat gatgccgatc tcggcgacga ggcccccgcc aagccgcgac
     8761 cgcatgcgca tgtcgagctt gtcgagctgg gtcggcgccc ggtctgccgc caccaccacc
     8821 tgccgtccgc cgtcgatcag cgaattcagc gtgtggcaga actcctgctg gatggtcgga
     8881 ccttgcagga actccatgtc gtcgatcagc agaatgtcga tcttgcgcag cttctccttg
     8941 aaggcgagcg cgtcgcgcgc gcgcaaggcc tgcacgaagc ccgacatgaa acgctcggcc
     9001 gtgaggtaga gcacctgcgc ctcgggctta cgctgcttca cgtcccacga gatggcatgc
     9061 aggaggtggg tcttgccgag gcccacgctg ccatgcacga aaagcgggtt gaagagctgc
     9121 tgcggtgtcg agaaggcgcc ggcgacttct tgggcggccg catgtgccag ccggttcgag
     9181 gtcccgacga gaaatgtcgc gaaagtgagc cgcggatcga gcggcgatcc ctcgaagc
//
LOCUS       DATNAE010000107         2596 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_708373, whole genome shotgun sequence.
ACCESSION   DATNAE010000107 DATNAE010000000
VERSION     DATNAE010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708373"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VN518_02270"
     CDS             <1..282
                     /locus_tag="VN518_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXE03430.1"
                     /translation="VAGIMWGSSLGVQFSISLVALKALPVLILGGFTSVPGAIVGGLM
                     VGAGEKVAEVFWGPATGGAIEDWFAYVLALVFLLFRPQGLFGERIIERV"
     gene            286..1365
                     /locus_tag="VN518_02275"
     CDS             286..1365
                     /locus_tag="VN518_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009467584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXE03431.1"
                     /translation="MLYREAGQFKTSYAADQAVFPIRQDRIFIAVLLLVAYAAIPWIA
                     NDYWLLTILIPFLVFSLAALGLNILTGYAGQVSLGTGGFMAVGAFATYKLATSFPGLN
                     ILAVFIIAGLITALVGLAFGFPSLRIKGFYLAVATLAAQFFLIWMFNKFPWFNNYTAS
                     GVISAPPRAIFGKVFVTGAEADPVHKYLFVLSFVALLAFAAKNLVRGRIGRSWMAIRD
                     MDIAAEIIGIRPLRTKLLAFAVSSFYVGIAGALWAFIYTGGVEPLAFDIDRSFQILFM
                     IIIGGLGSILGSFLGAAFIVLLPIFLDNAPRLVGLDINVSTSTNLQSMIFGALIVFFL
                     IVEPRGLARLWQIAKEKLRMWPFPY"
     gene            complement(1489..1710)
                     /locus_tag="VN518_02280"
     CDS             complement(1489..1710)
                     /locus_tag="VN518_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03432.1"
                     /translation="MSRADDAERARRKAHDLLSGIQRKDQERLAARDRLREAEALKTA
                     RLRELRLARDAAHKADKESVGTPPVRGDR"
     gene            complement(1747..1950)
                     /gene="rpsU"
                     /locus_tag="VN518_02285"
     CDS             complement(1747..1950)
                     /gene="rpsU"
                     /locus_tag="VN518_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HXE03433.1"
                     /translation="MQVFVRDNDINSALRVLKKKMQREGTFREMKRRRHYEKPSERRA
                     REKSEAIRRNRKALRKRLEREGY"
     gene            complement(1980..2237)
                     /locus_tag="VN518_02290"
     CDS             complement(1980..2237)
                     /locus_tag="VN518_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03434.1"
                     /translation="MARKTQSVPDGFVLFDVVYEDGTLSSNRRVARAQIDQLDADASA
                     QAAIEAQDRKIAEMSGRPLVPIKSVTRSGSRQRGAPPAASR"
     gene            complement(2251..2412)
                     /locus_tag="VN518_02295"
     CDS             complement(2251..2412)
                     /locus_tag="VN518_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03435.1"
                     /translation="MPFKPNYSSRRADRRRAQQQKQEEKQQRRQQRAAERKVEREEPP
                     PTSDGSSEH"
BASE COUNT          350 a          884 c          796 g          566 t
ORIGIN      
        1 gtcgccggca tcatgtgggg cagcagcctc ggcgtgcagt tctcgatctc gctggtggcg
       61 ttgaaggcgc tgccggtgct gatcctcggc ggcttcacct cggtgccggg cgcgatcgtc
      121 ggcggcttga tggtcggcgc gggcgaaaaa gtcgccgagg tcttctgggg gccggcgacg
      181 ggcggcgcca tcgaggattg gtttgcctat gtgctggcgc tggtcttcct gctgttccgg
      241 ccgcagggcc tgttcggcga gcgcatcatc gagcgggtct gagcgatgct ctaccgcgaa
      301 gccggtcagt tcaagacgag ctacgccgcc gaccaggcgg tgttcccgat ccgccaggac
      361 cgcatcttca tcgcggtgct gctgctcgtg gcatatgccg ccataccgtg gatcgccaac
      421 gactattggc tgctcaccat cctgatcccg ttcctcgtct tctcgctcgc cgccctcggg
      481 ctcaacatcc tgacgggcta tgccgggcag gtctcgctcg gcaccggcgg cttcatggcg
      541 gtcggcgcct ttgccaccta caagctcgcc acgtctttcc ccggcctcaa catcctcgcg
      601 gtcttcatca tcgccgggct gatcaccgcg ctggtcggcc tcgccttcgg cttcccgagc
      661 ttgcgcatca agggcttcta cctcgccgtg gcgacgctcg cggcgcagtt cttcctcatc
      721 tggatgttca acaagttccc gtggttcaac aactacaccg cctcgggcgt gatcagcgcg
      781 ccgccgcgcg ccatcttcgg caaggtgttc gtcaccggtg ccgaagccga tcccgtgcac
      841 aaatacctct tcgtgctgag cttcgtggcg ctcctcgcct ttgccgccaa gaatttggtg
      901 cgcggccgca tcggccgctc atggatggcg atccgcgaca tggacatcgc cgccgagatc
      961 atcggcatcc gcccgctgag aacgaagctg ctggctttcg cggtgagctc tttctatgtc
     1021 ggcatcgccg gcgcgctctg ggcgttcatc tataccggcg gcgtcgagcc cctcgccttc
     1081 gatatcgacc gctcgttcca gatcctgttc atgatcatca tcggcggcct cggcagcatc
     1141 ctcggatcgt tcctcggcgc cgccttcatc gtgctgctgc cgatcttcct cgacaacgcg
     1201 ccgcgcctcg tcggcctcga catcaatgtc agcacctcga ccaatctgca gtcgatgatc
     1261 ttcggcgcgc tcatcgtctt cttcttgatc gtcgagccgc gtggcctggc gaggctgtgg
     1321 cagatcgcca aggaaaagct ccggatgtgg ccgttcccgt attagggcgc gtcctcggga
     1381 gcgagccgaa ggccgccctt gttcgatgtc cgcaatcgag gatgtgcgac tatcggaaac
     1441 ctgccaaagc ctcgaggctc ctgaggcgag tcacgcttgg tcgcgcgctt agcgatcgcc
     1501 acgtacgggc ggtgtcccaa cactctcttt gtcggcctta tgcgcagcgt ccctggccag
     1561 ccgcagctcg cgcaggcggg cggtcttgag cgcttcggcc tcgcggagcc tgtcgcgcgc
     1621 cgccaaccgc tcctgatcct ttcgctggat tccgctcaga aggtcgtgcg ccttgcggcg
     1681 ggcgcgctcg gcgtcatcgg ctctgctcac ggcggctctc cttattcgac gcggcggcgg
     1741 acccgctcaa tatccctcgc gctccaagcg ctttctcagg gctttgcggt tccggcgtat
     1801 tgcctcggat ttttctcgcg cgcgtcgctc agagggcttc tcatagtggc gccgccgctt
     1861 catctcgcgg aacgttccct cgcgctgcat ctttttcttg agcacgcgga gcgcagaatt
     1921 gatgtcgttg tcgcgtacga acacctgcaa atcatctgct cctgatcggt catcgagcct
     1981 cagcgcgacg ccgcgggcgg tgcgccgcgc tgccgggacc ccgagcgggt aaccgacttg
     2041 atgggcacga gcgggcggcc ggacatttcg gcgatcttgc gatcctgcgc ctcgatggcc
     2101 gcctgcgccg atgcgtcggc gtcgagctga tcgatctggg cgcgtgcaac ccttcggttc
     2161 gagctcaagg tcccgtcctc gtaaacgacg tcgaagagga cgaagccatc cggtaccgac
     2221 tgtgtcttac gcgccatgat gctattcagc tcaatgttcg gaggaaccat ccgaggttgg
     2281 cggaggctcc tcgcgttcga ccttgcgttc tgcggcgcgt tgctggcgac gttgctgctt
     2341 ttcttcctgc ttttgctgct gcgcccggcg acgatcggcc cgccgcgaac tatagttcgg
     2401 cttgaacggc atggcttctc ctttcaccag cgttgcctcg ccggcgctct cgcccagggc
     2461 agacgggcgg gctcgggcgg tagacggccg agcgaaaatt gctacgccgg cttgaggttc
     2521 tccgccgacg atttcccgcg cttcggatcg cgctgttctt cgtaggatat ccgctgcccc
     2581 tcctgcagcg aacgca
//
LOCUS       DATNAE010000108        13160 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3418765, whole genome shotgun sequence.
ACCESSION   DATNAE010000108 DATNAE010000000
VERSION     DATNAE010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 13160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13160
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3418765"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..962
                     /locus_tag="VN518_02300"
     CDS             <1..962
                     /locus_tag="VN518_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1974 domain-containing protein"
                     /protein_id="HXE03436.1"
                     /translation="TFAQGALRSHPYLFTEIEALQDPDQDLGVPAFERAFDGHVAFTV
                     SNIFGALFHNLTLGAFAGAPPNAGATRRWWKQLARASRSFALVADATVALLGGGLKVR
                     QKITGRMADALTELYLLSCILKRYEDDGCPRADLKLVDYCAENCLYRFDKALAGTLDN
                     FPMRWVGLLMRPLVFPYGLARNPASDSAGKAIVRAALQPGEFRDRLTRDIFNTDDPSD
                     RVGLLEHTLIKVIAAEEADKKLERAIRKGDVHRYYNNDWIAEAEKKGVINEDEARMLA
                     EIRDLTARVIAVDHFDPADVSHRQPAQHAPASFAPPKDPIAAE"
     gene            970..2961
                     /locus_tag="VN518_02305"
     CDS             970..2961
                     /locus_tag="VN518_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HXE03437.1"
                     /translation="MPTPLPTLHDWRLSVDYEGIAWALMDREGERVNSMGRRFTEELG
                     EIVKWAETAAIHGEIKGLVLMSAKPTGFLVGADIREFSRFATEAQVKEVVKESLQLFD
                     SIEKLPIPVVSAIHGFCLGGGLELALACHYRIADREESTRLGFPEVKLGIFPGLNGTV
                     RSIQLAGPMDAMTAMLTGRMLRPTAAKAMGLIDQVVATHHNLRWAARKAVLQKRRSKG
                     IPWWKQMMLKQPVRSLLAKQMRAKTAEKVREDHYPAPYRLIGLFEQYGDDPARMRTAE
                     TEIFAPLMASDASRNLRRVFMLSEMLKDEAPKNGFKPKFVHVVGAGTMGGDIAAWCVV
                     CGMQASLQDLDEGQLKKALARAKGLFKRQLRGKAAIDAAAARLIADPKGKYIKHADVV
                     IEAIVEKLEVKQKLFQDLEAEMKPGAVLATNTSSLSIEDIAKPLKDPGRLVGIHFFNP
                     LAQLPLVEVIRGKDTREEEVRKATAFVAAIKKLPLIVKSCPGFLVNRVLTPYMMEAVR
                     RYAEGTPRDKIDQAALKFGMPMGPLELMDLVGLDIANKVGAELGSRTPPDNPLSRLVQ
                     AGKLGKKTGEGFYVWENGKAKRGETKYDDAELDRLGRELIKPMLDECERALSDGIVAN
                     ADHVDAGVIFGTGFAPFRGGPLHYRRAQEAPAAAAPAAA"
     gene            2987..4300
                     /locus_tag="VN518_02310"
     CDS             2987..4300
                     /locus_tag="VN518_02310"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HXE03438.1"
                     /translation="MSDKGMRRVAVLGGVRIPFCRSHTAYAELSNLDMLTAALTGLID
                     RFSLHGVHIDEVVGGAVVTHAKDWNLAREAVIGSTLAPTTPGITMMQACGTSLQGALG
                     LGAKIATGQIDCGIALGSDTTSDAPIVFKRSFAQRLVQLSRARSFGEKLGVFKGFTPA
                     ELAPQPPSTSEPRTGLSMGEHCELMAKEWEITREAEDLLAYESHKKAAAAYDRGFMDD
                     LVIPCAGVFRDNNLREDISLEKMAELKPVFDKAGGTITAANSTPLTDGASTVLLASEE
                     WAAKRGIPVQAYLTLGRTAAVDYVAGEGLLMAPTVAVRDLLKESNLTLQDFDFYEIHE
                     AFAAQVLATLKAWESDDYCRNRLGRDRALGSIDGAKLNVKGSSIAFGHPFAATGARIL
                     AVLAKLLHTEGGCRGLISVCTAGGMGVAAILEGTESREPARGTMP"
     gene            4434..5615
                     /locus_tag="VN518_02315"
     CDS             4434..5615
                     /locus_tag="VN518_02315"
                     /inference="COORDINATES: protein motif:HMM:NF021438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HXE03439.1"
                     /translation="MKHRTFAPAIGALAALFVAAGVPSYASAADPSGIWAKDDGSAKM
                     EVKKCGRGICSKIVWLRDPNDSRGRPLHDARNENTSMRDRPIIGLPLFANMVAIDSST
                     WQGSVYNPEEGKIYTDVKVTLAARNQIVLKGCKAWLLCGEKYWTRSKLPPTEAEPEPI
                     EVKAPAEPVEPADQAAPDGQKPIEVKATEPPPMDMAPKAPMDMASKVPMDIAPKSKPE
                     PAPIVEAKRQPVAEPPAPKPIAEPLAAEPSSEATTVEARVSVAPPKQRTTSPAASQHA
                     VGLGLVTTPTSHPDPLPLTGEDVSSMIVMTKPAPVAATPAPAAPTPEVSEANVESAAV
                     DNEPPAPPPAAKPKPKTLSADASESASAAGEPRVVAVKPKPKVKPKEPEEVLPWLRPR
                     Y"
     gene            complement(5630..7369)
                     /locus_tag="VN518_02320"
     CDS             complement(5630..7369)
                     /locus_tag="VN518_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HXE03440.1"
                     /translation="MARPELTPKCSRARQNSPGGDYPWLKAYPKDIDWGMEIRPVLLT
                     SLLDDAVAAYGSQTCTYFMGKRLSFAEIGAWSDQAAKGLQALGVGEGVKVGLLMPNCP
                     TFVVFYHAILKAGGTVVNFNPLYSQEEIELQVRDSGTKVMVTLDLAATFDKVEALLAS
                     GALDKAVVASFAPLLPKLKAVGMKLRRGPKLAQIGKSPQKAKIVAEKDLLANDGNYRR
                     PAIGTDAIAVLQYTGGTTGTPKGAMLTHANLSINVKQVMAWRGQTMQAGDRIIGVLPL
                     FHVFAMTTVMNFGIGQGMEMILLPRFELIETLKLITKLKPKMMPGVPTLFNAMLQYPR
                     IRDFDLTSLEFCISGGAGLPLEVKRGFEAMCSCTLTEGYGLSETSPVAACNPHDRPPR
                     EGSIGLPLPGTEFSIRSLEDPSVEVERGEAGEICIAGPQVMAGYWNKPDETESAFVGR
                     FFRTGDVGYMDEEGFIFIVDRIKDMINASGFKVYPRRIEDVLYEHPAVEEATVVGIPD
                     AYRGEAPKAFVKLREGERATQAELLEFLRPRLSKIELPAEIEFRDELPKTLVGKHSKK
                     ELRAERAAKSRSA"
     gene            7754..8023
                     /locus_tag="VN518_02325"
     CDS             7754..8023
                     /locus_tag="VN518_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HXE03441.1"
                     /translation="MDDVATKIIEILRKNMKDPSKAVTLETPLSELEIESLDLAVIVF
                     DIEDTFGIEIPYNANEEVEAFATVGSVVDRVKSIIAETKASGAAA"
     gene            8084..9322
                     /locus_tag="VN518_02330"
     CDS             8084..9322
                     /locus_tag="VN518_02330"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HXE03442.1"
                     /translation="MTHSNGRRRVVVTGQGVVTPLGTGVDRFWAALKNGECGIRQVQS
                     FSTEDLYITIAGEIPDFDPKERLHSKPLMLADKYSQYAGCAAHEAVAQSKLELPLKDN
                     ESYRSACIIGSGVGGLTTLEFSYKMLFKENKRATHPLTLLKAIGSSASAHVSIEYGIK
                     GPTFGVVSACATATHAIGLTYRMIREGLVDFGIAGAAEASLNWGATRAWQAMRVLSPD
                     GLFPFAKRRNGTVLAEGAGILMLEEYERAKARGAPILAELIGYGMTADAADMVNPSID
                     GASMAMQMALDDAAVSPGDIDYINAHGTATAVNDINETRAIKRVFGAAANKLSISSTK
                     SMHGHCLGAGGGIEAVAAVKAIEEKFVPATVGLDEVDPECDLDYTPNVGRKREVNYAM
                     SNSFAFGGLNAVLVFGPPPA"
     gene            9453..9947
                     /locus_tag="VN518_02335"
     CDS             9453..9947
                     /locus_tag="VN518_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03443.1"
                     /translation="MRRLGAIIVTTFCVSFAVTPARALGDLQNCDAIANAGERMACLY
                     AHIQHLEQTLLSLSTDLIDLRHELKEKLAADGVYKLQYVGRGSCLNFADNNKPPMMAS
                     CDHPDSWKLVRGTQSPGSESAAPKTPSPDQPDKAADKGKGKDKKKKQQGETDQPKAAP
                     PQPN"
     gene            complement(9944..10621)
                     /gene="radC"
                     /locus_tag="VN518_02340"
     CDS             complement(9944..10621)
                     /gene="radC"
                     /locus_tag="VN518_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013500065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HXE03444.1"
                     /translation="MGFKDAGKPPYLGHRERLRRRFRESGADALPDYELLEMILFRAV
                     PRRDTKPLAKAILAHFGSFTEALNAPEERLKEVPGLAITEIKLVRAAALRLMRGEVLE
                     RPVLASWSQVLDYCRASMGFEAKEQFRILFLDKRNQIIADEVQQKGTVDHTPVYVREV
                     VKRALELSATAIVLVHNHPSGNPTPSRADIEMTRQIVSSAKNLGIVVHDHIIVGKQGH
                     ASGLGLI"
     gene            complement(10632..11456)
                     /gene="map"
                     /locus_tag="VN518_02345"
     CDS             complement(10632..11456)
                     /gene="map"
                     /locus_tag="VN518_02345"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HXE03445.1"
                     /translation="MSNLEFARAQARDTKIKLHGPDAFAGMHKAGQLVAEALDLLVDE
                     VKPGVTTEALDALVFDFAMDHGAVPAPLNYRGFPKSICTSLNHVVCHGIPGPRVLKEG
                     DVLNIDVTFIVDGWHGDSSRMYAVGKISRAAERLLEVTYNALMRGVAAVRPGATTGDI
                     GAAIQAYAESERCSVVRDFCGHGLGRVFHDRPNILHYGEPGEGLQLKPGMLFTIEPMI
                     NLGKPPVKILSDGWTAVTRDRSLSAQFEHTVGVTETGCQVFTLSPKGLDRPPLTRQ"
     gene            11620..12399
                     /locus_tag="VN518_02350"
     CDS             11620..12399
                     /locus_tag="VN518_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HXE03446.1"
                     /translation="MTDSHQSSDTVTAALIIIGEEILSGRTRDENISHIARYLTEIGI
                     VLQEVRVVRDVEAEIVAAVNALRARYSYVFTTGGIGPTHDDVTTDAIASAFGVGVEID
                     ERAVEAMQKGYSQLELTPARLRMARIPHGAELVDNPVSRAPGFMLGNVIVMAGIPRIM
                     QVMLDAVAPRLKKGRPMLSRSIRIEAPEGDVAPGLAAIQAAHEDVQIGSYPFFEKAKL
                     GTYVVLRSVDTTALDRVHGALRELIAKERFKASEGEAEGKS"
     gene            complement(12491..12625)
                     /locus_tag="VN518_02355"
     CDS             complement(12491..12625)
                     /locus_tag="VN518_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03447.1"
                     /translation="MAVAKKAAAKKAVKRKVVAKKAKTAAVKRGARRKVAKKAAVKRK
                     "
BASE COUNT         2483 a         4202 c         4131 g         2344 t
ORIGIN      
        1 tcaccttcgc ccaaggggcg ctccgcagtc acccttatct cttcaccgag atcgaggcgt
       61 tgcaggatcc cgatcaggat ctcggcgtgc cggcgttcga gcgcgccttc gacggccatg
      121 tggccttcac cgtgtcgaat atcttcgggg cgctcttcca taacctcacc ctcggcgcct
      181 ttgccggcgc cccgcccaac gccggcgcga cgcggcgctg gtggaagcaa cttgcccgcg
      241 cctcgcgctc cttcgccctc gtcgccgacg cgaccgtggc gctgctcggc ggcgggctga
      301 aggtgaggca gaagattacc ggccgcatgg ccgatgcgct gaccgagctt tacctcttgt
      361 cttgcatctt aaagcgctat gaggacgacg gctgcccgcg cgccgacctc aagctcgtcg
      421 actactgcgc cgagaattgc ctctaccgct tcgacaaggc tcttgccggc acgctcgaca
      481 atttcccgat gcgttgggtc ggcctcctca tgcggcccct ggtgtttccg tacggccttg
      541 cccgtaaccc cgcatcggac agcgccggca aggcgatcgt gcgggcggca cttcagccag
      601 gagagttccg cgaccgcctg acccgcgata ttttcaacac tgacgatccg agcgatcggg
      661 tcggcctgct ggagcatacc ttgatcaagg tcattgccgc cgaggaggcc gacaagaaac
      721 tcgagcgtgc gattcgcaag ggcgacgtgc accgctacta caacaatgac tggatcgccg
      781 aagccgagaa gaagggcgtg atcaacgagg acgaagcgcg catgctcgcc gagattcgcg
      841 atctcacggc ccgcgtgatc gccgtcgatc atttcgaccc ggcggacgtg tcgcaccggc
      901 agccggcgca gcatgcgccg gcgagtttcg ctcccccgaa agacccaatt gccgccgaat
      961 aggatcacca tgccgacgcc gctccccaca cttcacgact ggcgcctctc cgtcgactac
     1021 gagggcatcg cctgggcgct catggaccgc gaaggcgagc gggtgaactc gatggggcgc
     1081 aggttcaccg aggagctcgg cgagatcgtg aaatgggctg agaccgccgc catccacggc
     1141 gagatcaagg gtctcgtgct gatgagcgcc aagccgaccg ggtttctcgt cggcgccgac
     1201 atccgcgaat tctcccgttt cgccaccgaa gcgcaggtca aggaggtggt gaaggagtcg
     1261 cttcagctgt tcgacagcat cgagaagctg ccgatcccgg tggtctcggc cattcatggc
     1321 ttctgcctcg gcggtgggct tgagctcgcg ctcgcctgcc actaccgcat cgccgaccgc
     1381 gaggagagca caaggctcgg cttccccgag gtgaagctcg gcattttccc cggcctcaac
     1441 ggcacggtgc ggtcgattca gctggccggc cccatggacg ccatgaccgc catgctcacg
     1501 gggcgcatgc tgcgtccgac ggcggcgaag gccatgggcc tgatcgatca ggtggtggca
     1561 acgcatcaca atctccgctg ggcggcgcgg aaagcggtgc tgcagaagcg ccgctccaag
     1621 ggcattccct ggtggaagca gatgatgctg aagcagccgg tgcgctcgtt gctcgccaag
     1681 cagatgcgcg ccaagacggc ggagaaagtg cgcgaggacc attaccccgc gccttaccgg
     1741 ctgatcggcc tcttcgagca gtacggcgac gatcccgccc gcatgcgcac ggccgaaacc
     1801 gagatcttcg cgccgctgat ggcgagcgac gcctcgcgca atctccgccg cgtgttcatg
     1861 ctctccgaga tgctgaagga cgaggcgccg aagaatggct tcaagccgaa attcgtgcac
     1921 gtcgtcggcg ccggcaccat gggcggcgac atcgccgcct ggtgcgtggt gtgcggcatg
     1981 caggcgagcc tgcaggacct cgacgagggc cagctcaaga aggcgctcgc ccgcgccaag
     2041 gggctgttca agaggcagct ccgcggcaag gctgcgattg acgctgcggc cgcgcgcctc
     2101 atcgccgacc cgaaaggcaa gtacatcaag cacgccgatg tggtgatcga ggcgatcgtc
     2161 gagaagctcg aggttaagca gaagctgttc caggacctcg aagccgagat gaagccgggc
     2221 gccgtgctcg ccaccaacac gtcatcgctc agtatcgagg acatcgccaa gccgctgaaa
     2281 gatccaggcc ggctcgtcgg catccatttc ttcaatcccc tggcacagct tccgctggtc
     2341 gaggtgattc gcggaaagga cacgcgtgag gaggaggtac gcaaagccac cgccttcgtc
     2401 gccgccatca agaaactgcc gctgatcgtg aagagctgcc ccggcttcct cgtcaaccgc
     2461 gtgctgaccc catacatgat ggaggcggtg cgccgttacg ccgaaggaac gccgcgcgac
     2521 aagatcgatc aggcggcgct caaattcggc atgccgatgg ggccgctcga gctcatggac
     2581 cttgtcggtc tcgacatcgc caacaaggtc ggagccgagc ttggctcccg cacgccgccg
     2641 gacaatccac tgtcgcgcct cgttcaggcc ggcaagctcg gcaagaaaac gggcgaaggt
     2701 ttctatgtgt gggagaacgg caaggccaag cgcggcgaga cgaaatacga cgacgccgag
     2761 ctcgatcgcc tcggccgcga gctgatcaag cccatgctcg acgaatgcga gcgcgcgctt
     2821 tcagatggga tcgtggccaa cgccgatcac gtcgatgccg gcgtgatctt cggcaccggc
     2881 tttgcgcctt tccgcggcgg gccgctgcat tatcggcgcg cgcaagaagc acccgcggcc
     2941 gcagctccgg ccgcggcctg agcgagcagg gagactccaa gccatcatga gtgacaaagg
     3001 gatgcggcgt gtcgcggtgc tcggcggggt gcgaatcccg ttctgccgct cgcatacggc
     3061 ctatgccgag ctatcaaatc tcgacatgct gaccgcggcg ctcaccggcc tgatcgaccg
     3121 gttctcgctg cacggcgtgc atatcgatga ggtggtcggc ggtgccgtcg tcacccacgc
     3181 caaggactgg aacctcgcgc gcgaagcggt gatcggcagc acgcttgccc ctaccacccc
     3241 cggcatcacc atgatgcagg cctgcggcac gagcctgcaa ggcgcgctcg ggctcggcgc
     3301 caagatcgcc accggccaga tcgactgcgg cattgcgctc ggctccgaca ccacgagcga
     3361 tgcgccgatc gtgttcaagc ggagcttcgc ccagcggctg gttcagctct cccgcgcgcg
     3421 aagcttcggc gagaagcttg gtgtgttcaa aggcttcacc ccggccgagc ttgcccccca
     3481 gcctccctca acctccgagc cgcgcaccgg cttgagcatg ggcgagcact gcgagctgat
     3541 ggccaaggaa tgggaaatca cccgcgaggc tgaagacctg cttgcctatg aaagccacaa
     3601 gaaggcggcc gccgcctacg accggggttt catggacgac ctcgtgattc cctgcgcagg
     3661 ggtgttccgc gacaacaacc tccgcgaaga catctcactt gagaagatgg cggagctgaa
     3721 gcccgtcttc gacaaggccg gtggcaccat caccgccgcc aactcaacgc cgctcaccga
     3781 cggcgcctcg accgtgctgc tcgcctccga ggaatgggct gcgaagcgtg gcattccggt
     3841 tcaggcctat ctgacgcttg ggcgcaccgc cgccgtcgac tacgtggcgg gcgaggggct
     3901 tctgatggcc ccgaccgtcg ccgtcaggga cctcctgaag gagagcaacc tgacactaca
     3961 agactttgat ttttatgaaa ttcatgaggc cttcgccgcc caggtgctgg ccaccttgaa
     4021 ggcctgggaa tcggacgact attgccgcaa ccgcctgggg cgggaccggg cgcttggctc
     4081 gatcgacggg gcaaagctca acgtcaaagg ctcgagcatc gccttcggcc atccttttgc
     4141 cgcgaccggc gcccgcattc tggcggttct cgccaagctg ttgcatactg agggcggctg
     4201 tcgcggcctg atttcggtgt gcacggcagg gggcatgggc gtcgcggcga tcctggaagg
     4261 aacggaatcg cgggaaccgg cgcgggggac aatgccttga aatggcgtag accaagggcc
     4321 gcggagccat acaaaggcct tataaaggcc cgattaggaa ccgggggcgt aagcttaaat
     4381 attgccggcg tcatttgcac cgtcggcgcg gcagttttgg aggaggtgct tttatgaagc
     4441 atcgcacatt cgccccggcc atcggagccc tggccgccct gtttgtggca gccggcgtgc
     4501 cctcttatgc ctccgctgcc gacccatccg gcatttgggc caaggatgac ggatcggcga
     4561 agatggaggt taagaaatgc ggccgcggca tctgtagcaa gatcgtctgg ctgagggacc
     4621 ccaacgattc gcgcgggcgg ccgctgcatg acgcccggaa cgagaatacc tcgatgcgcg
     4681 accggccgat catcggcctt cccttgttcg ccaacatggt ggcgatcgat tcgagcacct
     4741 ggcagggcag cgtctacaat cccgaagagg gaaagatcta taccgacgtc aaggtgacgc
     4801 tcgctgcgcg caaccagatc gtgctcaaag gctgcaaggc ctggctgctc tgcggcgaga
     4861 aatactggac gcggtccaag cttccgccta ccgaggccga gccagagccc attgaagtga
     4921 aggcgccggc cgagccggtt gagccagccg atcaagctgc cccggacggg caaaaaccga
     4981 tcgaggtcaa agcgacagag ccgccgccta tggacatggc tccgaaggcg cccatggata
     5041 tggcttcgaa ggtgcccatg gatatagctc cgaagtctaa gcctgagcct gcgcccatcg
     5101 tggaggccaa gcgacagccg gtcgccgaac cgccggcgcc gaagccgatc gccgagccgc
     5161 ttgctgcgga gccgtcatcg gaagcgacaa ccgtcgaggc tagagtctca gtcgctccgc
     5221 ccaagcaaag aacaacgtcg cccgcagcct cccagcacgc cgtggggctt gggctggtca
     5281 ccacgccgac aagtcacccc gatccattgc cgctcaccgg cgaggacgtc tcgagcatga
     5341 tagtgatgac caagcccgcg ccagtggcgg ctacgccggc acctgccgcg ccgacgcctg
     5401 aggtcagcga agcgaacgtc gaatcagccg ccgttgacaa cgagccaccg gcgcctccgc
     5461 ccgccgcaaa gccaaagccg aagacgctgt cggccgatgc ctccgagtcc gcctctgcag
     5521 cgggagagcc gcgcgtggtc gcggtgaagc caaagccaaa ggtgaagcca aaggagcccg
     5581 aggaggttct gccctggctt cggccgcgct actaagcagt cgcattcact taagctgatc
     5641 ggcttttcgc cgcccgctcg gcgcggagct ctttcttgga gtgcttgccc acgagcgtct
     5701 tggggagctc atcgcggaac tcgatctcgg ccggcaactc gatttttgac agcctcggcc
     5761 ggagaaactc gagcagctca gcttgcgtgg cccgctctcc ctccctcaac ttgacgaagg
     5821 ccttcggcgc ctcgcctcga taggcgtcgg gaatgccgac caccgtcgcc tcttcgaccg
     5881 ccggatgttc gtagagaaca tcttcgatcc gtctcggata gaccttgaag ccggaggcgt
     5941 tgatcatgtc cttgatgcgg tcgacgatga agatgaagcc ctcttcgtcc atgtagccga
     6001 cgtcgccggt gcggaagaag cgtccgacga atgcgctctc ggtctcgtcc ggcttgttcc
     6061 agtagcctgc catgacctgc ggcccggcaa tgcagatctc gccggcttcg cctcgttcga
     6121 cttccacact cgggtcctcg agcgagcgaa tgctgaactc cgtgcctgga agcggcaggc
     6181 cgatcgatcc ttccctcggg ggacggtcat gcggattgca ggctgcgacc ggcgaggttt
     6241 cgctcaaacc gtacccctcg gtcagcgtgc agctgcacat cgcctcgaag ccgcgtttca
     6301 cctcgagcgg cagcccggcg ccgccggaga tgcagaactc gagcgaggtg agatcgaagt
     6361 ctcgaatccg cggatactgc agcatggcgt tgaacagggt tggcacgccc ggcatcattt
     6421 tcggcttgag cttggtgatc agcttcaagg tctcgatcag ctcgaagcgc ggcaagagga
     6481 tcatctccat gccctggccg atgccgaaat tcatcaccgt ggtcatggcg aagacgtgga
     6541 agagaggcag cactccgatg atgcgatcgc ccgcctgcat cgtttgcccg cgccaggcca
     6601 tcacctgctt cacattgatg gagagattgg cgtgggtgag catggcaccc ttgggcgttc
     6661 ccgtggtgcc gccggtatat tgcagcacgg cgatcgcgtc cgtgccaatc gcgggccttc
     6721 gatagttgcc gtcattggca agcaggtcct tctcggcgac gatcttggcc ttctgcggcg
     6781 acttgccgat ttgggcgagc ttcggcccgc ggcgaagttt catgcctacg gccttcagct
     6841 tcggcagaag cggggcgaag cttgccacca ccgccttgtc gagggcgccg ctcgcgagca
     6901 gagcctcgac cttgtcgaag gtggcggcaa gatcgagcgt caccatgacc ttggtgccgc
     6961 tgtcgcggac ctggagttca atctcctcct ggctgtacag gggattgaag ttgacgacag
     7021 tgccgccggc cttgaggatc gcgtgataga aaacgacaaa cgtcgggcag ttgggcatca
     7081 acagccccac tttcacaccc tcgccgacgc cgagcgcctg gaggcccttg gccgcctggt
     7141 ccgaccaagc gccgatctcg gcaaagctca gcctcttgcc catgaaatag gtgcaggtct
     7201 gagagccgta ggccgcgacg gcgtcgtcga gcaggctcgt gagcaggacc ggcctgattt
     7261 ccatccccca gtcgatgtcc ttggggtagg ccttcagcca cggataatcg ccgccgggac
     7321 tgttctgcct ggcccgcgaa cactttgggg ttaattcggg tctagccatg acgcaggtgc
     7381 cgctttagcg tctttttagc ctctttccgg ttaactggcc gggaagcggc ggaaaaattg
     7441 gaggcaattg gccgcagggc ctctcattca ccagaatcga ccccctacta tatcacggcc
     7501 tgaagccgaa agccggggaa gcggtccggc ggacccggct tcgcgattat gctgaaaccg
     7561 tggcgcggta ggccaaaaaa ggccaatttt gttggcgatg agagcccgca atgggcggct
     7621 gaatcgagca aggacgccgt agactcggct agtttttaag tctattccaa tacctgactt
     7681 gcccttggta acgctatgaa ttagcgggag ggcctttgga cacggacccc gcaaacgggg
     7741 cagggagagc gatatggacg atgtggctac caagatcatc gagattctgc gcaagaacat
     7801 gaaggacccg tcgaaggcgg tcacgctgga aacacccttg agcgagcttg aaatcgaatc
     7861 gcttgatctc gcggttatcg tgttcgatat tgaagataca ttcgggatcg agatccctta
     7921 taatgcgaat gaagaggtag aagcgttcgc gacggtggga tccgtcgtcg atcgcgtgaa
     7981 gagcattatc gcagagacga aggcttcggg cgcagccgct tgataggcct ttccgcccgg
     8041 gacctacctg ggcattgaaa tctccggagt tacattagct ggcatgacac attctaatgg
     8101 gcgccgccgc gtcgtggtca ctggccaggg cgtggttact cctttgggca ccggcgtgga
     8161 tcgtttttgg gctgcgctca agaacggcga gtgcggcatt cgccaagtcc aaagcttctc
     8221 caccgaggat ctctacatca ccatcgccgg tgagatccca gacttcgatc cgaaggaacg
     8281 gctgcattca aagccgctca tgctcgccga caaatattcg caatatgccg gctgcgcggc
     8341 gcatgaagca gtcgcccaat ccaagctcga gctgccgctc aaggacaacg agagctaccg
     8401 ctcggcctgc atcatcggct ccggcgtcgg tggtctgacc acgctcgaat tttcctacaa
     8461 aatgctgttc aaggagaata agcgcgccac ccatccgctg accctgctca aggcgatcgg
     8521 cagctcagcg tccgcccatg tcagtatcga atacggcatc aagggcccga ccttcggcgt
     8581 ggtgagcgcc tgtgcaaccg cgacgcacgc gatcggcctg acctaccgca tgatccgcga
     8641 gggcctcgtc gatttcggca ttgccggcgc ggccgaggct tcgttgaatt ggggcgccac
     8701 gcgcgcctgg caggcgatgc gggtgctgag ccctgacgga ctgtttccgt tcgccaagag
     8761 gcggaacggc accgtgctcg cggaaggcgc aggcattctc atgctcgagg aatacgagcg
     8821 cgccaaagcc cgcggcgctc ccatcctcgc cgagctgata ggctacggca tgacggctga
     8881 cgccgccgac atggtcaacc cttcgatcga cggtgcgtcc atggccatgc agatggcgct
     8941 tgacgatgct gccgtgtccc cgggcgatat cgactatatc aacgcgcacg gcacagcgac
     9001 cgccgtgaac gacatcaacg agacccgcgc catcaagcgc gtgttcggcg ccgccgcgaa
     9061 caagctttcg atctcctcca ccaaatccat gcatggtcat tgtctcggcg ccggcggcgg
     9121 catcgaggcg gtcgcagcgg tcaaggccat cgaggagaaa ttcgtgcccg ccacggtcgg
     9181 cctcgacgag gtcgatcccg aatgcgacct cgactacacc ccgaacgtgg ggcgcaagcg
     9241 cgaggtcaac tacgccatgt ccaactcctt cgccttcggg ggcctgaacg cggtcctcgt
     9301 gtttggaccg ccgccggctt aatccggcct ttcccgcctc tcgaccgcgc tgacattttc
     9361 cgccttaaag cggaagatat tcggcttgcg ccgcgaccat cctctggcaa tggatggggc
     9421 ttgacccaag agagatcgag gaggcgaggg acatgcgtag gctcggagcg attatcgtca
     9481 ccacattttg cgtaagtttc gcggtaaccc cggcgcgcgc gctcggcgac ctgcaaaact
     9541 gcgacgccat cgccaatgcc ggcgagcgga tggcttgcct ctacgcccac atccagcacc
     9601 ttgagcagac cttgctcagc ctcagcaccg atcttatcga tctcaggcac gagctgaagg
     9661 agaagctcgc cgccgacggc gtctacaagc tgcaatatgt ggggaggggc agttgcctca
     9721 actttgccga caacaacaag ccgccgatga tggcgagctg cgaccacccc gattcctgga
     9781 agctcgtacg cggaacgcaa tcgccggggt cggaaagcgc cgcccccaag acgccgtcgc
     9841 cggatcagcc tgacaaagct gccgataagg gcaaaggcaa agacaagaag aaaaagcagc
     9901 aaggcgagac cgatcagccg aaggctgcgc cgcctcagcc gaactagatc agcccgaggc
     9961 cgctcgcatg gccctgcttt ccgacgatga tgtggtcatg caccacgatg ccgagattct
    10021 tggccgacga cacgatctgc ctggtcatct cgatatcggc tcggctcggt gtggggttgc
    10081 ctgaggggtg attgtgcacg agcacgatcg ctgtcgccga cagctcgagc gcccgcttca
    10141 ccacctctcg aacatagacc ggggtgtgat cgacggtgcc cttctgctgc acctcatcgg
    10201 cgatgatctg gttgcgcttg tcgaggaaca ggatgcgaaa ctgctccttg gcctcgaagc
    10261 ccatcgaggc gcggcaataa tcgagcacct ggctccacga ggcgagcacc ggccgctcca
    10321 gcacctcgcc gcgcatgagc cggagcgccg ccgcgcgcac gagcttgatc tcggtgatgg
    10381 cgaggcctgg cacttccttc aggcgctcct caggcgcgtt cagcgcctcg gtgaagcttc
    10441 cgaaatgggc aaggatggct ttggccagag gcttggtgtc gcgtcgcggc acggcgcgga
    10501 acaggatcat ctcgagcagc tcgtagtcgg gaagcgcatc ggcgccggac tcgcggaagc
    10561 gtcgccgcaa tctctcgcga tggccgagat agggtggctt gccggcgtcc ttgaagccca
    10621 ttcaggccac tctattgccg agtgagtggt ggccggtcca atcctttggg ggagagggtg
    10681 aacacctggc atcccgtctc agtgacgccg accgtgtgct cgaactgcgc cgaaagcgac
    10741 cggtcgcggg tgaccgcggt ccagccgtcc gacaggatct tcaccggcgg cttgccgaga
    10801 ttgatcatcg gctcgatggt gaagagcatg ccgggcttga gctgcaggcc ctcgcctggc
    10861 tcgccgtaat gcaggatgtt gggccggtcg tggaacacgc ggcccaagcc gtgcccgcag
    10921 aaatcgcgca ccacgctgca ccgctcagat tcggcatagg cctggatggc ggcgccgata
    10981 tcgccggtgg tggcgcccgg cctcaccgcg gcgaccccgc gcatcaacgc gttgtaggtg
    11041 acctcgagca ggcgctcggc ggcgcgggaa atcttgccca cggcgtacat gcggctcgaa
    11101 tcgccgtgcc agccgtcgac gatgaaggtc acgtcgatat tgaggacgtc gccctccttc
    11161 agcacgcgcg gtcccgggat gccgtggcag accacgtggt tcagcgaggt gcagatcgat
    11221 ttggggaagc cgcgatagtt gagcggcgca gggacggcgc catggtccat ggcaaagtcg
    11281 aagacgagcg cgtcgagcgc ctcggtggtc acgcccggct tgacctcgtc gacgagcagg
    11341 tccagcgcct cggccacgag ctggccggcc ttgtgcatgc cggcgaaggc gtcggggcca
    11401 tgcagcttga tcttggtgtc acgcgcctgg gcgcgggcga attcgaggtt cgacatcggt
    11461 cacgctttag gaaatgagtg atcacttcac ttaaggtgaa atggcccaat gtgcaacgtc
    11521 cgtggctgcc gcatgcaagg gtggccggga cctgcctgtt gcgaagccaa gcaagcccgg
    11581 ctgtgaatgg tcgaaggttc ctgctaagtg acctgggcca tgaccgatag ccatcagagt
    11641 tcagacaccg tcaccgccgc gctgatcatc atcggcgagg agatcctttc gggccgcacc
    11701 agggacgaga acatttccca catcgcccgc tacctcaccg agatcggcat tgtacttcag
    11761 gaggtgaggg tggtgcgcga cgtggaggcc gagatcgtcg ccgccgtgaa cgcgttgcgg
    11821 gcccgctaca gctacgtgtt caccaccgga ggcatcggcc cgacccatga cgacgtgacc
    11881 accgacgcca tcgcctcggc cttcggggtc ggggtcgaaa tcgacgaaag agcggtcgag
    11941 gccatgcaaa agggttatag ccagcttgag ctgaccccgg ccaggctgcg catggcccgc
    12001 atcccgcatg gcgccgagct cgttgacaac ccggtctcca gggcgcccgg cttcatgctc
    12061 ggcaacgtga tcgtgatggc cggcatcccg cgcatcatgc aggtgatgct cgacgccgtg
    12121 gcaccacgct taaagaaggg acggccgatg ctctcccgct caatccgcat cgaggcgccg
    12181 gaaggcgacg tggcgccggg cctcgcggcg atccaggccg cgcatgaaga cgtgcagatc
    12241 ggctcctatc cgtttttcga aaaggccaag ctcggcactt acgtcgtgct gcgctcggtg
    12301 gacacgactg cgctcgaccg cgtgcacgga gcgctgcgcg agctgatcgc caaggagcgc
    12361 ttcaaagcct ctgaaggcga ggctgagggg aagtcctaag gttttgggat tgcgccgata
    12421 ttcgaggccg ttgcctcgcc cttcggcgat gggcaggcgc gggagctcgc gctcccgcgc
    12481 ccttgacaag ctatttacgt ttcacagcgg ctttcttcgc aaccttgcga cgggcgccgc
    12541 gcttaactgc agcagtcttg gccttcttgg ccacgacctt cctcttcact gccttcttcg
    12601 ctgctgcctt ctttgctact gccatagtgc atccctccta tcgggctggc tggtagttca
    12661 ctctagctac atccactcgt cgcttcgcac gcatcgcatt cgagacacgt gcatcgcgca
    12721 cgaaggaaaa gctctgcatt gttcacgcac tttccttcat aggtcttaac gcagggctca
    12781 tcgataagaa cgtcgagtcg cgtttcggtg agtgattcgc gatccgaatt tttcctatcg
    12841 taatatttcg catactgcat cgcgcggcga tgtacaacta tttctgcact ccgcgcgcga
    12901 gcgctccggt aaccacccga gcgcggaatc gactgacgag agtgatcgag gcgcgtcgtt
    12961 ttcattccgg tcgcgaagag cgcgatcgcc gatcgcatcg cgcaacgaac ggcgacgcag
    13021 cgaacattca gcctgtgcga ttgcagtaac ggagcggcga tgcatggcga agagcgtgtc
    13081 tccatttagc tgcagccgct cgtcgagccg caggtatcgc atttgagaca cgtgccgttg
    13141 cgcacgagcg tgaagtttcc
//
LOCUS       DATNAE010000109         2318 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3118760, whole genome shotgun sequence.
ACCESSION   DATNAE010000109 DATNAE010000000
VERSION     DATNAE010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3118760"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..853
                     /locus_tag="VN518_02360"
     CDS             <1..853
                     /locus_tag="VN518_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03448.1"
                     /translation="AHTGYYFQEDMATWLAGSPTPTSFDHGMRNTWQALAEAAYPTRA
                     YASHMPQIVNKSLVNEIFDRFVTSRDAPAYDEWSLYFNVASQLYPRHFGAKPYGTLGW
                     PMRPGDWLPELEPLDPAFENYYPQNYEAAEGGMFAGLKPLGDLAAKTERAAAALRQAR
                     RAELEGGGVHAPSQLALIVAPDVLKFVGPGTILAGARNVRRILLLNGAADVTLVTGRL
                     DMFVTEPRGTPVRGESVRLGDACWIPLLPPNKPGLYAIGFFAALDSGARIEARGLLTV
                     VADEGRE"
     gene            934..1278
                     /locus_tag="VN518_02365"
                     /pseudo
     CDS             934..1278
                     /locus_tag="VN518_02365"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            1309..1776
                     /gene="bcp"
                     /locus_tag="VN518_02370"
     CDS             1309..1776
                     /gene="bcp"
                     /locus_tag="VN518_02370"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007563263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HXE03449.1"
                     /translation="MSLEVGDKAPSFTLPADGGGKVALKDLKGKKVVLYFYPKDDTSG
                     CTAEACAFRDNLPDFSKVKAAIVGISRDSVASHDKFKTKYKLPFPLVSDEDGKVCEAY
                     GTWVEKSMYGRKYMGIDRATLLIDEKGVIRGVWRKVKVPGHAEEVLKAAAALK"
     gene            complement(1811..2318)
                     /locus_tag="VN518_02375"
                     /pseudo
     CDS             complement(1811..2318)
                     /locus_tag="VN518_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340076.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="L-lactate permease"
BASE COUNT          468 a          722 c          753 g          375 t
ORIGIN      
        1 ggcgcatacc ggctattatt tccaagagga catggcgact tggcttgcag gctcgccaac
       61 gccgacaagc ttcgatcatg gcatgcgcaa cacttggcag gcgctcgccg aggccgccta
      121 cccaacccgc gcctatgcga gccacatgcc gcagatcgtc aataagagcc tggtcaacga
      181 aatattcgac cggttcgtga cgagccggga cgcacccgct tacgacgagt ggtcgctcta
      241 cttcaacgtc gccagccaac tttatccgcg gcatttcgga gcgaagccct atggcacgct
      301 tggctggccg atgcgcccag gggactggct gccggagctc gagccgctcg atcccgcctt
      361 cgagaactac tatccgcaga actatgaggc ggccgaaggc ggcatgttcg caggcctcaa
      421 gcctctcggc gatctcgccg cgaagacgga gcgcgcggct gcggcactga ggcaagcgcg
      481 ccgtgccgag ctcgaaggcg gcggcgtgca tgcgccaagc cagcttgccc tcatcgtcgc
      541 gcctgacgta ctgaaattcg tcggcccagg caccatcctt gcgggagcgc gtaacgtgcg
      601 gcgcattctc ctcctcaacg gggcggcaga tgtcactctc gtgacaggca ggctcgatat
      661 gttcgtcacc gagcctcgcg gcacgccggt cagaggcgaa agcgtgcgtt tgggcgacgc
      721 atgctggatc ccgctcttgc ctcccaacaa gcccggccta tatgccatcg gattcttcgc
      781 cgcactcgat tccggtgcgc ggatcgaggc gcggggcctg ttgacggtgg ttgctgacga
      841 ggggcgcgag tgaccgaggt tgacgacatc gcactggccg aagcgccata cggctccgac
      901 ctctatgccc aggctctaaa gctccgcgag gcgatgctgc ggaagccact cgggcttacc
      961 ctaagccagg aagagttggc cgacgatgcg ctgaggcggc atttctgcgc cctcaccaat
     1021 gggaccgtag tggggtcggt atccttcaag cccttgggac cgcacacgct ccagctcaag
     1081 cagatggcgg tggcgaaagc gcgccagcgc gaaggcgtgg gatcgcggct gctcgccttt
     1141 gccgaggctt gggcgcggcg cggcggctat ggcatgatca tcctcaacgc ccgtgtcggc
     1201 gtggaggatt tctacacgag acatggctac gcggccgaag gcgagccctt cgacgagaac
     1261 accatcccgc atatcaggat gacgaagcgg accttaggag ggaaacgcat gagtctcgag
     1321 gttggcgaca aggcgccgag tttcacactg cctgccgatg gcggcggcaa ggtcgcgctc
     1381 aaggacctca agggcaagaa ggtcgtgctc tacttctatc caaaggacga cacctcgggc
     1441 tgcacggcag aggcctgcgc ctttcgcgat aatcttcccg acttctccaa ggtgaaggcg
     1501 gcgatcgtcg gcatctcgcg cgactcggtc gcgagccacg acaagttcaa gacgaaatac
     1561 aagctcccct tcccgctcgt ctccgatgaa gacggcaagg tgtgcgaggc ctacggcaca
     1621 tgggtggaga agagcatgta tggacgaaaa tacatgggca tcgaccgggc caccctcctc
     1681 atcgacgaga agggcgtgat ccgcggcgtc tggcgcaagg tgaaagtccc tggccacgcc
     1741 gaagaggtgc tgaaggccgc ggcagcgctg aaatagggcg gccgctcggt tttgagggac
     1801 ccgggcctcg tcagtggaca agcagggtga gcggccagag ataggcctga cccaagacca
     1861 ggacgccgac cagcacggcg agcgccacgg aatggaaaaa cacgaagcgc agaatcgagc
     1921 cttcgtggcc gtactatttg gtcgcggtcg aggcgacgac gatcgactgc gcgtcgatca
     1981 tcttgcccat gacgccgcca gagctgttcg ccgcggccat cagcaccgga ttgatcccga
     2041 gctgctcggc ggtcattttc tgcaatccgc cgaagagaac gttcgacgag gtgtctgacc
     2101 cggtgagcgc cacgccgagc cagccgatca gcggcccgaa aaacggatag gaccagccgc
     2161 tattggcgag cgccaggccg agcgtcgcat cgctgcccga gtagcgcgtg atgaagccga
     2221 tggccaacat gcaggagatg gtgagcaagg aatagcgcac gaggtagatc gtgcgcccat
     2281 acatgcgggc gagccccagc ggccggtagc cgaggaag
//
LOCUS       DATNAE010000110         2329 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_1916100, whole genome shotgun sequence.
ACCESSION   DATNAE010000110 DATNAE010000000
VERSION     DATNAE010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1916100"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="VN518_02380"
     CDS             complement(<1..181)
                     /locus_tag="VN518_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009339877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXE03450.1"
                     /translation="MQISRLCLAAGFAAIAFFAPAVAAEDVSAGQEIYQAQCSACHSN
                     QPGVNGIGPSLAGVAG"
     gene            complement(322..1185)
                     /locus_tag="VN518_02385"
     CDS             complement(322..1185)
                     /locus_tag="VN518_02385"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HXE03451.1"
                     /translation="MAKSQSRIAFQGEPGANSHLAARDAYPRMEALACPTFEDALAAV
                     KTGEAKLAMIPIENSVAGRVADIHHLLPHSGLYIVAEHFERVRHQLLTLPGVKPAELK
                     TVHSHVMALGQCRKAIRALKLKAVVAADTAGAARELRESGDRTAAAIASRLAAEIYGL
                     NVARADIEDAEHNTTRFVVLAAKPVRVKKDDGPVVTSFVFRVRNVPAALYKALGGFAT
                     NGVNMTKLESYQLEGSFNATMFYADIEGHPDARPVALALEELHFFSSEVTVLGTYPAS
                     PFRAELKALEG"
     gene            complement(1213..1953)
                     /locus_tag="VN518_02390"
     CDS             complement(1213..1953)
                     /locus_tag="VN518_02390"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HXE03452.1"
                     /translation="MMPCLIVIPARLKATRLPDKPLADIGGEPMIVHVWRRAVEADCG
                     PVLVATDAEAVRDAILQAGGEALMTRPDHPSGSDRVFEAVLARDPHGKLGAVVNLQGD
                     LPTLDPALVHACVDALNEGEADIGTICATIVRDEERSDPNVVKVVGTPLGAGPVLRAL
                     YFTRATAPYGDGPLYHHIGIYAYRREALERFVSLPPSPLELREKLEQLRALEAGMRIN
                     VGLVDTVPLGVDTPRDLERARELLRPRP"
     gene            2111..>2329
                     /locus_tag="VN518_02395"
     CDS             2111..>2329
                     /locus_tag="VN518_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HXE03453.1"
                     /translation="MKHYRLNQIAMAVLGALLLFFGAKTLINIAYEEHEPEQPGYVVP
                     GTEEHPGGEVKPAGGDQLATLLASADPTR"
BASE COUNT          422 a          737 c          781 g          389 t
ORIGIN      
        1 gtcccgcaac gccggcgagc gaagggccga tgccgttgac gccgggctga ttggaatgac
       61 aggccgagca ttgcgcctga tagatttcct ggccggcgct gacgtcctcg gcagcgactg
      121 ccggggcaaa aaaggcgatc gcggcgaaac ctgcggctaa gcaaagacga gagatttgca
      181 tggcatgaac tgcgagaggc gcgcggccag tcggcggctg cgcggcgctt cttccacgtg
      241 cctagtctga tgaggatcag ttcggagctg aaacggcgcg ttgctctgaa aacgatagcg
      301 ccgtgctatg gcggccgagg cttagccttc caaggccttc aattcggcgc ggaacgggct
      361 cgccggatag gtgccgagca cggtcacctc gctggagaag aagtgcagct cctcgagcgc
      421 gagcgccacg gggcgcgcgt cggggtggcc ctcgatatcg gcatagaaca tggtggcgtt
      481 gaacgagcct tcgagctggt aggactcgag cttggtcatg ttgacgccat tggtggcgaa
      541 gccgcccagc gccttgtaga gcgcggccgg gacgttgcgc acgcggaaca cgaagctggt
      601 gacgaccggg ccgtcgtcct tcttgacgcg caccggcttg gcggcgagca cgacgaagcg
      661 cgtggtgttg tgctcggcgt cctcgatatc ggcgcgtgcc acgttcagcc catagatctc
      721 ggcggcgagc ctggaggcga tcgccgcggc cgtgcggtcg ccggactcgc gcaactcgcg
      781 ggcggcgccg gcggtatccg ccgccaccac cgccttcaat ttcagcgcgc ggatcgcctt
      841 acggcattga ccgagcgcca tgacatggct gtgcaccgtc ttgagctcgg cgggcttgac
      901 gccgggaagg gtcaagagct ggtggcgcac ccgctcgaaa tgctcggcca cgatgtagag
      961 gcccgaatgc ggcaacagat gatggatgtc ggcgacgcgg ccggccaccg aattctcgat
     1021 gggaatcatg gcgagcttgg cctcgccggt cttcaccgcg gcaagcgcgt cctcgaaggt
     1081 cggacaggcg agcgcctcca tgcgcggata ggcatcgcgc gcggccagat gcgagttggc
     1141 acccggctcg ccctgaaagg cgattctgct ctgcgatttg gccatggtct taaggaaagg
     1201 cgggctttta ggctaagggc ggggtcgaag cagctcacgc gcccgctcga ggtcgcgcgg
     1261 agtatcgaca ccgagcggca cagtgtcaac gaggccgaca ttgatgcgca tgccggcttc
     1321 gagcgcacgc agctgctcca atttctccct gagctcgagc ggcgacggcg gcaacgacac
     1381 gaagcgctcc aatgcctccc ggcgataggc gtagatgccg atatggtgat agagcgggcc
     1441 atcgccgtaa ggcgcggtcg cccgagtgaa atagagcgcc cgcagcaccg ggccggcgcc
     1501 aagcggcgtt cccaccacct tcaccacgtt gggatcgctg cgctcctcat cgcgaacgat
     1561 cgtcgcgcag atggtgccga tatcggcctc gccttcgttg agagcgtcca cgcaggcgtg
     1621 gaccagggcg ggatcgagcg tcggcaggtc gccctgcaaa ttaaccacgg cgccaagctt
     1681 accgtgcgga tcgcgcgcca gcacggcctc gaacacccgg tcggacccgc tcggatggtc
     1741 cgggcgcgtc atcaaggctt cgccgccggc ctgcagtatc gcgtcccgaa cggcttccgc
     1801 gtcggtcgcc accagcacag ggccgcaatc ggcctccacc gcgcggcgcc agacatgcac
     1861 gatcatcggc tccccgccga tatcggccaa gggcttatcg gggagtcttg tcgctttcag
     1921 ccgggcgggt ataacgatga ggcagggcat catgatctgc gtcagttagc atgggaaatt
     1981 ttcccaggcg accgtcgcgc ctatgtcaaa tgtccccgtt gcaacaagcc tataggctct
     2041 atagtctcac gccccgttgc aagagctaca tgaagtagcc tctggcctgg ccccgggggg
     2101 aaagtcgacc atgaagcatt atcgtctcaa tcaaatcgcc atggccgtgc tgggcgcact
     2161 gctcctgttc tttggcgcga agactctcat caacatcgcc tacgaggaac atgagccgga
     2221 acagcccggc tatgtggtcc cgggcaccga ggagcatccg ggcggcgagg tgaagccggc
     2281 cggcggcgat caattggcca cgctgcttgc cagcgccgat ccaacccgc
//
LOCUS       DATNAE010000111         2941 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2217263, whole genome shotgun sequence.
ACCESSION   DATNAE010000111 DATNAE010000000
VERSION     DATNAE010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2217263"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="VN518_02400"
     CDS             complement(<1..585)
                     /locus_tag="VN518_02400"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010659878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HXE03454.1"
                     /translation="MSDALKLGLAGVGTVGSGLLRLLAARKAELAARAGRPIEVVAYS
                     ARSRQKKRDANLTGLRFVQDPVALAQDPAIDVFVELIGGEGGVAKEAVEAALSAGKHV
                     VTANKALLAHLGASLAALAEKNQVALNFEGAVAGGIPVVKVMRESLQGNAVSRVYGIL
                     NGTCNYILTLMEQEGRSFADVLKEAQEQGYAEADP"
     gene            complement(594..1826)
                     /locus_tag="VN518_02405"
     CDS             complement(594..1826)
                     /locus_tag="VN518_02405"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HXE03455.1"
                     /translation="MTSDFHRIKRLPPYVFEQVNKLKAEARARGDDIIDFGMGNPDMP
                     TPEHITAKLMETLRDPRTNRYSASKGIQGLRRAQAAYYARRFGVKLNPDTQVVATLGS
                     KEGFANVAQAITAPGDVILVPNPTYPIHEFGFIMSGGVIRHLPARTDSQFMASLDKAV
                     RHSVPKPSALVLNYPANPTAVVADLDFYREVVAFAKKHDLIVLSDLAYAEIYFEGEPP
                     PSILQIPGAMDIAVEFTTLSKTYAMPGWRVGFAVGNERLIHALTRVKSYLDYGAFTPI
                     QVAAAAALNGPQDCVDEIRAIYKRRRDCLVDAITRAGWPIPAPPATMFAWAPIPEMFA
                     HLGSLEFSKLLLEKAGVAVSPGVGFGEYGEGYVRLALVENEHRIRQAARNIRRFLSDS
                     AKTMHNVIPIASKANRSF"
     gene            complement(1927..2190)
                     /locus_tag="VN518_02410"
     CDS             complement(1927..2190)
                     /locus_tag="VN518_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03456.1"
                     /translation="MLRALPSYASRLLALLIPLGLAACADTISSSEVTPSSELLKEYD
                     KTLTKSEQKAAISDLEAAQAKARGEASPESTGATGAKPAQTQD"
     gene            2443..>2941
                     /locus_tag="VN518_02415"
     CDS             2443..>2941
                     /locus_tag="VN518_02415"
                     /inference="COORDINATES: protein motif:HMM:NF018827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HXE03457.1"
                     /translation="MMRLFDQGTKAFSALAERTNSNGQGPYSMASEAGEAAKSLGEIA
                     RHWAAEPAKFATAQSELLASYADLWGRSVRRFLGEEVEPVVEPEPGDNRFKDPDWSNG
                     QVFDFWKQAYLITSRWAEELTRNTSGVDDKTRKKALFYLNQMLSAMSPSNFPLTNPEV
                     IRTTLA"
BASE COUNT          541 a          907 c          966 g          527 t
ORIGIN      
        1 cgggtcggcc tcggcatagc cttgctcctg cgcctccttc aagacgtcgg cgaagctccg
       61 accctcctgc tccatgagcg tcaggatgta attgcaggtg ccgttgagaa tgccgtagac
      121 gcgggagacg gcattgcctt gcaagctctc gcgcatcacc ttgaccacgg gaatgccgcc
      181 ggccacggcg ccctcgaaat tcagcgcgac ttggttcttc tcggcgagcg cggcgaggct
      241 ggccccgaga tgggcgagca gcgccttgtt ggcggtgacg acatgcttgc cggccgagag
      301 cgcggcctcg accgcctcct ttgccacacc gccctcgccg ccaatcagct caacgaacac
      361 gtcgatcgcc ggatcctgcg ccagagcgac gggatcttgc acgaaacgca gtccggtcag
      421 attggcgtcg cgtttctttt gccggcttct ggccgagtac gcgacgacct caattgggcg
      481 cccggcgcgt gcggcaagct ccgccttgcg cgccgccagc agccgcaaga gaccgctgcc
      541 gacggtgccg acccccgcca gcccaagctt caatgcatca ctcatgggag aaatcagaac
      601 gaccgattcg ccttggaggc aatgggaatg acgttatgca tggtcttggc cgagtcggag
      661 aggaatctcc gaatgttgcg cgcggcttgg cggatgcggt gctcgttctc gaccagcgcc
      721 agtcggacat agccctcgcc atattcgccg aagccgaccc cgggcgagac cgcgacgcca
      781 gccttttcga gcaggagctt cgagaattcg aggctgccga gatgagcgaa catctcgggg
      841 atcggcgccc aggcaaacat cgtggccggc ggcgccggga tgggccagcc ggcgcgcgtg
      901 atggcatcga ccaggcaatc gcgtcttcgc ttgtagatgg cgcgaatctc atcgacgcag
      961 tcctgcgggc cgttcagcgc cgcggcggcg gccacctgga tcggggtgaa ggcgccatag
     1021 tcgagatagg acttcacgcg ggtgagcgca tggatcaggc gctcattgcc gacggcgaag
     1081 cccacgcgcc agccgggcat ggcgtaggtc ttggacagtg tggtgaactc gaccgcgatg
     1141 tccattgcgc ccggaatctg caggatggag ggcggcggct cgccctcgaa atagatctcg
     1201 gcgtaggcaa gatcggagag cacgatcaga tcgtgcttct tggcgaaggc gacgacttcc
     1261 cgatagaagt cgagatcggc gacgaccgcg gtcgggttgg ccggataatt gagcacgagc
     1321 gctgacggct tcggcaccga atggcgcacc gccttgtcga gcgaggccat gaactgggaa
     1381 tcggtgcggg ccggcaagtg cctgatgacg ccgcccgaca tgatgaagcc gaactcgtgg
     1441 atcggatagg tcggattcgg cacgaggatc acgtcgccgg gggcggtgat cgcctgcgcc
     1501 acgttggcga aaccttcctt cgagccgagc gtggccacca cttgcgtatc ggggttgagc
     1561 ttcacgccaa agcggcgcgc gtaataagcg gcctgcgccc tgcgtaggcc ctggatgccc
     1621 ttcgacgccg agtaacggtt ggtgcgcgga tcgcggagcg tctccatcag cttggcggtg
     1681 atgtgctccg gcgtcggcat gtcgggattg cccatgccga aatcgatgat gtcatcgccg
     1741 cgcgcccgtg cctcggcctt gagcttgttc acctgctcga agacgtaagg cggcaaccgc
     1801 ttgattctat ggaaatcgct ggtcacgacg cgaggggtcc taccccgttt aggcccctaa
     1861 gggacatcac ttgaaccata aaaatgggcc gtggggcaag gcccgccggg ccttcgccgc
     1921 aagctgctag tcttgcgtct gggccggttt tgcgccggtc gcgccggtcg attcggggct
     1981 cgcttcgccg cgggctttcg cctgggctgc ctcgaggtcg ctgatcgcgg ccttctgctc
     2041 ggacttggtc agggtcttgt catactcctt gaggagctcg ctcgacggcg tgacctcgct
     2101 cgaggagatg gtatcggcgc aagcggcgag gcccagcgga atgagcaggg ccaagaggcg
     2161 cgaggcatag ctaggaagcg cgcgcagcat gagtttggag cagtggtccc gccagttccc
     2221 cgtgacgatt ggcaatcgtc gtgcacttac tatattgcat agcacaatga ccccttgcgc
     2281 aacagtggcc gacgcgcccg agcctggtat tcgatggtgg ctgcagcaag gtaaactggg
     2341 ggttaaaaca aggacagatt gatgcaggag aagaataaaa cacccggccc gtccggggtt
     2401 gaacaagtct acgccaatca catggaagcc ttcgccagga atatgatgcg tctcttcgat
     2461 cagggcacca aggctttctc cgcgctggcc gagcggacca actcgaacgg ccaaggccct
     2521 tacagcatgg cctcggaagc cggcgaagcg gccaagtcgc tcggcgagat cgcgcggcat
     2581 tgggcggcag agcccgccaa gttcgccacc gcgcagagcg agttgctggc gagctacgcc
     2641 gatctctggg gccgttcagt ccgccgcttt ctcggcgagg aggtcgagcc ggtcgtcgag
     2701 ccggagccgg gcgacaaccg cttcaaggat cccgactggt cgaacggtca ggtcttcgat
     2761 ttctggaagc aggcttatct catcacctcg cgctgggccg aggaacttac ccgcaacact
     2821 tcgggggtcg acgacaagac gcgcaagaag gcgctgttct atttgaatca gatgctctcg
     2881 gcgatgtcgc cttcgaactt cccgctcacc aatccagaag tgatccgcac cacgctcgcg
     2941 a
//
LOCUS       DATNAE010000112         5382 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2824854, whole genome shotgun sequence.
ACCESSION   DATNAE010000112 DATNAE010000000
VERSION     DATNAE010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5382
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2824854"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..150
                     /locus_tag="VN518_02420"
     CDS             <1..150
                     /locus_tag="VN518_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008196280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme transporter HemC"
                     /protein_id="HXE03458.1"
                     /translation="PSLLTPLFVMAAGFTLLFVLLHLIAMRAEILRRRVQALRQVHVE
                     RAASA"
     gene            169..390
                     /gene="ccmD"
                     /locus_tag="VN518_02425"
     CDS             169..390
                     /gene="ccmD"
                     /locus_tag="VN518_02425"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HXE03459.1"
                     /translation="MPDLGPHAAFIWAAYAVTFIAVAALIVFIVQDDRRQRQLLADLE
                     RQGIRRRSAKPDDAPKQRQAKPRARKPKP"
     gene            387..974
                     /locus_tag="VN518_02430"
     CDS             387..974
                     /locus_tag="VN518_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020179616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HXE03460.1"
                     /translation="MIAEAARSFWQRWGLAFPLLIFALLSGLFWYALQSGDPSRLPSA
                     LVGKPVPAFALPPIEGLTGEGAQGFETADLAKGAPTIVNVWASWCVPCHEEHPLLLEL
                     AKQPGVRLYGINYKDDGAAARRFLGRYGNPFARVGADRSGRVAIDWGVYGVPETYVIT
                     GDGKIAYRHVGPLTEDAITQKLLPLVQPSGEKPQG"
     gene            complement(986..1426)
                     /locus_tag="VN518_02435"
     CDS             complement(986..1426)
                     /locus_tag="VN518_02435"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HXE03461.1"
                     /translation="MCAKNTKQLERSATHLIHRAGQRATDIFQAEARASGLTPRQFAV
                     LMTVADEEGLTQTDLVDRTGIDRSTLADIVARLLARGLIQRKRAKEDGRAYAIKLTSQ
                     GWKGLRDAEPGAAATDSRLLAVLPPAKRQEFLDILNTIVTAMNK"
     gene            complement(1669..2292)
                     /locus_tag="VN518_02440"
     CDS             complement(1669..2292)
                     /locus_tag="VN518_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HXE03462.1"
                     /translation="MPQQPEAAPGAGPQNAESGGALAIKLAIELGPLLVFFAANAAAG
                     IYAATACFMAVTVVALAVAWWRYHKLPMMLLVSAVVVFVFGTLTLYLHDETFIKLKPT
                     IVYLAFATFLGGGLLLKKPVLELLLGSVFNLTEPGWRKLTARWALFFIAMAVLNELVW
                     RNVSTDAWVSFKAFGFLPLTFLFALSQMPLMQRYSDAPADKDTGTKA"
     gene            complement(2276..3697)
                     /gene="ftsY"
                     /locus_tag="VN518_02445"
     CDS             complement(2276..3697)
                     /gene="ftsY"
                     /locus_tag="VN518_02445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HXE03463.1"
                     /translation="MSSEKKPGFWQRLFGIGAQEPPKEEPAPPKPARKEPPAPRPAQK
                     EPPKQKQPAKKAAKLKAPPPAGPHVAKDEGASPDGGPKGPKPGGGKRPRKPKAEAPAA
                     APPPAGPAAKEGARPPEERHPATREKATPDEPGVPQPAAEPEEPKDGKPSKASAPQTN
                     PQRSWFARLGQGLARTSTALSDNLASVVSRRKLDEETLDELEDALIKADLGVAMAGRI
                     RQALAATCHDRGLSPQALREVLAAEIAKVLEPVAKPLTLDASAHPHVILLVGVNGTGK
                     TTTAAKLAHQLMRGGSSVMLVAADTFRAAAIDQLKVWGDRVDAEVVAKDVGADPAGVA
                     YEALERARAEKCRALIIDTAGRLQNKADLMAELAKIVRVVKKLDPAAPHSVVLVLDAT
                     TGQNALNQVQIFQEIAGVTNLVVTKLDGTARGGILAAIAERFALPVSAIGVGEGVDDL
                     QPFDAQDFARAIAGLDTRNAAAA"
     gene            complement(3694..4950)
                     /gene="mtaB"
                     /locus_tag="VN518_02450"
     CDS             complement(3694..4950)
                     /gene="mtaB"
                     /locus_tag="VN518_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HXE03464.1"
                     /translation="MSDIEIITFGCRLNAYESEVMRKHACDAGLGATVIVNTCAVTQE
                     SVRQARQAIRRARRENPHAKIVVTGCAAQIDPARFAAMDEVDHVIGNGEKTEAQTFKG
                     LGCGDTERVKVNDIMSVKETAGHLIDGFGAQARAYVQIQNGCDHRCTFCIIPYGRGPS
                     RSVPAGEVVAQVRKLVENGYAEIVLTGVDITAYGADLPGELTLGRLVTKILKLVPELK
                     RLRLSSIDSVEADAALLKAIAEEERLMPHLHLSLQAGHDLTLKRMKRRHSRAHAVSFC
                     EEVRRLRPDMVFGADLIAGFPTESEEMFAGSISLIDDCELTFLHVFPFSARPGTPAAR
                     MRQLNGAEIASRAARLREKGVEALRRHLVNAKGRHIQVLMESKGRGRAGDFTPVKIEA
                     EEGAPKLIDAVVKGHDNSALIARTLV"
     gene            complement(4999..>5382)
                     /gene="dapF"
                     /locus_tag="VN518_02455"
     CDS             complement(4999..>5382)
                     /gene="dapF"
                     /locus_tag="VN518_02455"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HXE03465.1"
                     /translation="HCIFFVQNIEAHDLARFGPALEHDPLFPERANISLAQVTGPSSL
                     RLRTWERGAGLTRACGTAACAAAVAAARSGLTGRHVIVTLPGGDLVIDWRDADDHILM
                     TGPFALDYQGTIPPELLQPAPATSG"
BASE COUNT          898 a         1776 c         1728 g          980 t
ORIGIN      
        1 ccgtcgctgc tcacgccgct cttcgtcatg gcggctggct tcaccctgct cttcgtgctg
       61 ttgcacctca tcgccatgcg tgccgagatc ttgcgccgcc gggtgcaggc gctccgccag
      121 gtgcatgtcg agcgcgccgc ttccgcctga ggccgaaccc cgccgcccat gcccgatctc
      181 ggtccccacg cggcattcat ctgggcggct tatgccgtga ccttcatcgc cgtggccgcg
      241 ctcatcgtgt tcatcgtgca ggacgaccgg aggcagcggc agctgctcgc cgatctcgaa
      301 cggcaaggca tcaggcggcg ctcagccaag ccggatgacg cgcccaagca gcgtcaagcg
      361 aagccccggg cgaggaagcc aaagccatga tcgcagaagc agcacgctcg ttctggcagc
      421 gctgggggct cgcttttccg cttctgatct tcgccttgct gtcagggctg ttctggtacg
      481 cgctgcagag cggcgacccc tcgcggctgc cgtctgccct cgtgggcaag cccgtgccgg
      541 ccttcgcctt gccgccgatc gaggggctca ccggcgaagg tgcgcaagga ttcgagaccg
      601 ccgatctcgc caagggcgcg cccaccatcg tcaacgtgtg ggcctcgtgg tgcgtgccct
      661 gtcacgagga gcatcccctg ctgctcgagc ttgccaagca gccaggcgtt cggctttacg
      721 gcatcaacta caaggacgat ggcgcggcgg cgcgacggtt cctcggccgc tatggcaacc
      781 ctttcgcccg ggtcggcgcc gaccgctcgg ggcgcgttgc catcgactgg ggcgtctatg
      841 gcgtgcctga gacctatgtg atcacgggcg acggcaagat tgcctaccgc cacgtgggcc
      901 ctctcaccga ggacgccatc acacagaagc tgctgccgct ggtccagcct tctggagaga
      961 aaccgcaagg ctgacgaggg gcaggctatt tgttcatggc ggtgacgatc gtattcagga
     1021 tgtcgagaaa ctcctgccgc ttggccggcg ggagtacggc aagcagccgc gaatccgtgg
     1081 cggcggcgcc aggctcggcg tctcgcaggc ccttccagcc ctgcgaggtg agcttgatgg
     1141 cgtaggccct gccatcctcc ttggcgcgct tccgttgaat gagcccacgc gccaggagtc
     1201 ttgccacaat gtcggcgagg gtcgacctgt cgatcccggt gcggtccacg aggtcggtct
     1261 gagtgagccc ctcttcgtcg gcgacggtca taagcacggc aaattgccga ggcgtgagac
     1321 cgctggcgcg ggcctcggcc tggaaaatgt ccgtcgcgcg ttgaccggca cgatggatca
     1381 aatgcgtcgc cgaacgctcc aattgcttag tgtttttcgc acacatcctg acgctccgtc
     1441 tcttttcgca ggcatcaagc ccacgttgcc cctatgacgg taagatgact ttttccgcag
     1501 tttaacaaat tgaagacaat ttgttctaaa ccacagattg cagttgatcc ccatagacac
     1561 cttagcggtg tttagcgtga aggcaaaccc ccccacagcc ccggcgaaat attggtattg
     1621 gagcagccgc ctgcaagatc agaagcacat taagcttggg cttaatcttt aggcctttgt
     1681 cccggtatct ttgtcggccg gggcgtcgct atagcgctgc atgagcggca tctgcgacag
     1741 ggcgaagagg aacgtcagcg gcaggaagcc gaaggccttg aagctcaccc aggcgtcggt
     1801 cgacacattg cgccagacga gctcgttcaa gacagccatg gcaatgaaga acaaggccca
     1861 acgggcggtg agcttgcgcc agcctggctc ggtgagattg aacaccgagc cgagcaacag
     1921 ctcgagcacc ggcttcttga gcaaaaggcc gccgccaagg aacgtggcga aagcaagata
     1981 gacgatggtt ggcttcagct tgatgaaggt ctcgtcgtgc agataaagcg tgagcgtgcc
     2041 gaacacgaat acgacaacgg cactgaccag aagcatcatc ggcagcttgt gatagcgcca
     2101 ccaggccacc gcaagcgcga ctacagtcac cgccatgaag caagccgtcg ccgcatagat
     2161 gcctgccgcg gcattggcgg cgaagaacac gagtagcggg ccaagctcga tggcgagctt
     2221 gatggcaagc gctccgccag attccgcgtt ttgcggtccc gcccccggcg ctgcttcagg
     2281 ctgctgcggc attcctggtg tccagaccgg cgatggcgcg ggcgaaatcc tgagcgtcga
     2341 agggctgcaa atcgtcgacg ccctcaccga cgccgatggc gctcaccggc aaagcgaaac
     2401 gctcggcgat ggccgcaaga atgccgccgc gtgcggttcc gtcgagcttg gtcacgacca
     2461 ggttcgtcac tcctgcgatc tcctggaaga tctgcacctg gttcaacgca ttctgccccg
     2521 tggtggcgtc gagcacgagc accacgctgt gcggcgcggc aggatcgagc ttcttcacca
     2581 cgcgcacgat cttggcgagc tcagccatca gatcggcctt gttctgcagg cgcccagccg
     2641 tgtcgatgat gagcgcccgg cacttctcgg cgcgcgctcg ctcgagcgcc tcataggcga
     2701 caccggccgg atcggcgccg acatccttgg ccaccacctc ggcgtcgacg cggtcgcccc
     2761 ataccttcag ctgatcgatg gccgcagcgc ggaaagtgtc ggccgctacg agcatcaccg
     2821 aggaaccgcc gcgcatcaat tgatgcgcca acttggccgc tgtggtggtc ttgccggttc
     2881 cgttcacgcc gacgagcagg atcacatggg ggtgcgcgct tgcatcgagc gtcaaaggct
     2941 tggccaccgg ctcaagcacc ttggcgatct cggcggcgag cacctcgcgc aacgcttgcg
     3001 gcgacagccc ccggtcgtgg caggtcgcgg cgagcgcctg cctgatccgc ccggccatgg
     3061 cgacgccgag atcggccttg atcagcgcgt cctcgagctc gtcgagcgtc tcttcatcga
     3121 gctttctccg gctcaccacc gaagcaagat tgtcgctgag agcggtagag gtccgggcga
     3181 gcccctggcc gagccgtgcg aaccagcttc gctgggggtt cgtctgggga gctgaggctt
     3241 tcgagggctt gccgtccttt ggttcctcgg gctctgcggc aggctgaggg acaccgggct
     3301 cgtccggtgt ggctttttcc cgcgtcgctg ggtgtcgttc ctcgggcgga cgcgctccct
     3361 ccttcgcggc aggacctgcc ggcggtggcg cagctgccgg cgcctcggct ttcggcttgc
     3421 gcgggcgttt cccgccacca ggcttggggc ccttagggcc cccgtcaggg gacgcgccct
     3481 catccttggc gacatgcggg ccggccggcg gcggggcctt cagcttggca gccttctttg
     3541 ccggctgctt ttgctttggc ggctccttct gagccggcct tggcgccgga ggctctttcc
     3601 tggctggttt cggtggagcg ggctcttcct taggcggctc ctgcgcgcca atgccgaaca
     3661 gccgctgcca aaatcccggc ttcttctcgc tgctcacacc aacgtcctcg cgatcagagc
     3721 gctgttgtca tgacccttca ccacggcgtc gatcaatttg ggtgcgccct cttctgcctc
     3781 gatcttgacc ggcgtgaagt cgccggcgcg ccctcgtcct ttgctctcca tgagcacctg
     3841 aatatgccgg cccttcgcgt tcacaagatg gcgccgcaag gcctcgaccc ccttctcgcg
     3901 cagacgcgcc gcgcggctcg cgatctccgc gccgttgagc tggcgcatgc gcgcggcagg
     3961 cgtgcccggc cgcgccgaga atgggaagac gtgcaggaag gtgagctcgc aatcgtcgat
     4021 cagcgatatc gagccggcga acatctcctc gctctcggtg gggaagccgg cgatgaggtc
     4081 tgcgccgaac accatgtccg ggcgaaggcg gcgcacctcc tcgcagaaac tcaccgcatg
     4141 ggcccgcgaa tgccgccgct tcatgcgctt gagcgtgagg tcgtggcctg cttgcagcga
     4201 cagatggaga tgcggcatca gccgctcctc ttcggcgatg gccttcagca gtgccgcgtc
     4261 ggcctcgacc gaatcgatgg aggagagccg caagcgcttg agctcaggca cgagcttcaa
     4321 aattttggtc accagcctgc cgagcgtgag ctcgccggga aggtccgcgc cgtaagccgt
     4381 aatgtcgacg ccggtcagca cgatctcggc atatccgttc tcgaccagct tccgcacctg
     4441 cgccaccacc tcgcccgccg gcaccgagcg cgacggcccg cgcccataag gaatgatgca
     4501 gaaggtgcag cgatgatcgc agccgttctg gatctgcaca taggcgcggg cttgggcgcc
     4561 gaagccatcg atcagatggc ccgccgtctc cttcaccgac atgatgtcgt tgaccttgac
     4621 ccgctcggta tcaccgcagc cgagaccctt gaaggtctgc gcttcggtct tctcgccatt
     4681 gccgatgacg tggtcgacct cgtccatggc ggcgaagcgg gccggatcga tttgcgctgc
     4741 gcagccggtg accacgatct tggcgtgcgg attctcgcgg cgcgcccgcc gaatagcctg
     4801 ccgcgcttgg cgcacgctct cctgcgtcac cgcgcaggtg ttaacgatca ccgtggcccc
     4861 aagcccggcg tcgcatgcat gcttgcgcat gacctccgac tcataggcgt tgagcctaca
     4921 gccgaaggtg atgatctcga tatccgacat tggcggcttc taacacgaaa cgagcgatgc
     4981 gggcgagcca gatagccgct agcctgaggt cgcgggcgcc ggctgcagca gctcaggcgg
     5041 aatggtgcct tgatagtcaa gcgcgaaggg cccggtcatc agaatatggt cgtcggcatc
     5101 gcgccagtcg atgacgaggt cgccgcctgg cagcgtcact atcacatgcc ggccggtcag
     5161 cccgctgcgg gcagcggcca cggcggcggc gcaggccgcg gtgccgcaag cgcgtgtgag
     5221 cccggcgccg cgctcccagg tacggagccg caaggaggaa ggcccggtca cctgggcgag
     5281 cgagatgttg gcgcgctcgg gaaacagcgg atcatgctcg agcgcaggtc cgaagcgggc
     5341 aaggtcgtgc gcctcgatat tttggacgaa gaaaatgcag tg
//
LOCUS       DATNAE010000113         2790 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3123212, whole genome shotgun sequence.
ACCESSION   DATNAE010000113 DATNAE010000000
VERSION     DATNAE010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3123212"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..997)
                     /locus_tag="VN518_02460"
     CDS             complement(<1..997)
                     /locus_tag="VN518_02460"
                     /inference="COORDINATES: protein motif:HMM:NF014686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HXE03466.1"
                     /translation="MPRSLPADGASLLVAAVSSRALAASARRAGFVPLVADFFADADT
                     RQIAHSCHELDDLKGGFQWKALAPALEALAEAAPSPLLGVVYGSGFEDGPGLLAKIAE
                     RWPLLGNGPSVVERVKSPEVLFTELARLGIPHPRTVMDPAAAGSGWLAKRQGGAGGSH
                     IVTSLVAAASTARRRSIKGRTYFQEKLEGRPVSAQFAGAGTSAQVLGFSEQWAAPTSR
                     AKWRYGGAARPAELSPDLEARLTDSVERVAECFDLKGLGSADFLVNGEEFYLLEINPR
                     PGATLDIFDSEAEPLLRIHLEAVLENRLPTALRNLPAASAAAIVYATEPITVSQTM"
     gene            complement(975..1871)
                     /locus_tag="VN518_02465"
     CDS             complement(975..1871)
                     /locus_tag="VN518_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent
                     methylenetetrahydromethanopterin dehydrogenase"
                     /protein_id="HXE03467.1"
                     /translation="MAHPCILHMITPLKHMSPFDVNMALDAGYDATIPYTSVTVEDVT
                     GLVQDAIFSRGPEGLKRTAMFIGGKRAIEALDMMKRAKAAMVPPFEISVFADPAGSFT
                     TAAAMVACAEKALKDKFKTEFKGKRVVVFGGTGVVAFASAVISAVEGAEAVLVGYDGP
                     ERVRKLAEEANARFEVKLGYADGSTEEQKTELVRGAEVIFSAGPAGKRLLTTDQLAKA
                     THLLVATDVNAVPPAGVEGVGVNDNGVPIAGTKAVGIGALAVGNIKYKTEAGLFDQMI
                     QSDKPVYLDFRDAYALAQKLAG"
     gene            complement(1967..>2790)
                     /locus_tag="VN518_02470"
     CDS             complement(1967..>2790)
                     /locus_tag="VN518_02470"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HXE03468.1"
                     /translation="SLRRGSELKVTGVPCERAEPYLRSIAASFRLAASYHLHLDEVIP
                     GHAGLGSGTQLAVAIGSAVATLEGLPLDFDEIAARLDRGKRSGIGIGTFGLGGAVLDG
                     GPGPGKLPPVLARVPFPRAWRVLLIFDPAETGVHGAEEAAAFATLPDFPESETADLTR
                     RVLLDALPALAEEDFKAFCDQVGYLQKKMGAYFAPMQGGAYVSDGVATVLNGLAAYGV
                     TGLGQSSWGPTGFAFAPSEAEGQRLLAEARGMAGSAKLRFELAQGRNEPAIIEAR"
BASE COUNT          493 a          925 c          913 g          459 t
ORIGIN      
        1 tcatcgtttg tgacacagtg atcggttccg ttgcgtaaac gatggccgcg gcggaggccg
       61 ccgggaggtt gcggagggca gtcggaagcc ggttttctag cacagcctcg aggtggatgc
      121 gaagcagggg ctcggcctcg ctgtcgaaaa tgtcgagcgt ggcaccgggc cgcggattga
      181 tctcaaggag atagaattcc tcgccattga ccaggaaatc ggcgctgccg agccctttta
      241 gatcgaagca ttcggccaca cgctccacgc tgtcggtcaa acgcgcctcg agatccggcg
      301 atagctcggc gggccgcgcc gcgccgccat agcgccattt cgcacgtgag gtcggggccg
      361 cccactgctc gctgaagccg agcacctgag cgctggtccc ggctccggcg aactgggccg
      421 acaccgggcg gccctccaac ttctcctgaa agtaagtgcg gccttttatt gacctccgcc
      481 tggcggtgct ggctgcggcg acgagactcg tcacgatgtg acttccgccg gcgccgccct
      541 ggcgcttggc gagccagcca ctccctgccg cggcagggtc catgacggtt ctcggatgcg
      601 gaatgccgag ccgcgcaagc tcggtgaaga gcacctcggg cgacttcacc cgctccacta
      661 cgcttggccc gttgccgagc agcggccagc gttccgcgat cttggcgagc agcccaggac
      721 catcttcgaa gcccgagcca tagaccacgc cgaggagcgg cgacggcgcg gcctcggcca
      781 gcgcctcaag cgccggcgcg agagccttcc actgaaagcc gcccttcaga tcgtcgagct
      841 cgtgacagga atgcgcgatc tgcctcgtgt cggcatcggc gaagaaatcg gcgaccagcg
      901 ggacaaatcc ggcgcgcctc gctgaggctg cgagcgcgcg gctcgacacc gccgcgacga
      961 gcagggatgc tccgtcagcc ggcaagcttc tgggcaaggg cgtaagcgtc gcggaaatcg
     1021 agatagaccg gtttgtccga ctggatcatc tgatcgaaca ggccggcctc ggtcttgtat
     1081 ttgatgttgc cgaccgcgag cgccccgata ccgacggcct tggtccccgc gatcggcacg
     1141 ccgttgtcgt tcacgccgac gccctcgacg ccggcaggcg gcacggcgtt gacgtcggtg
     1201 gcaacgagga ggtgggtcgc tttcgcgagt tgatcggtgg tgagcagcct cttccctgcc
     1261 ggacctgccg agaaaatcac ctcggcgccg cgcacaagct cggtcttctg ctcttcggtc
     1321 gagccgtcgg cgtaaccgag cttgacctcg aaacgggcat tggcttcctc ggcgagcttg
     1381 cgcacgcgct caggcccgtc atagccgacc agaaccgcct cggcgccctc gacggcggaa
     1441 atgacggcgg aggcgaaagc aacgacgccg gtgccgccga acaccaccac gcgcttgcct
     1501 ttgaactcgg ttttgaactt gtccttcagc gccttctcgg cgcaggcaac catggccgcg
     1561 gcggtggtaa aggagcctgc cggatcggcg aacacggaaa tctcgaacgg cgggaccatc
     1621 gccgccttgg cccgcttcat catgtcgaga gcctcgatgg cacgcttgcc gccgatgaac
     1681 atggccgtcc gcttcaaccc ctcaggcccg cgggaaaaga tcgcgtcctg cacgagcccg
     1741 gtcacgtcct cgaccgtcac cgacgtatag ggaatggtgg cgtcatagcc tgcgtcgagc
     1801 gccatgttca cgtcgaacgg gctcatatgc ttgagcggcg tgatcatgtg caggatgcag
     1861 ggatgcgcca tttcgtcagg tctccgtcat ttttggctgg cacgctttac cacaaggggc
     1921 gtgacggaaa agcgtttcga tcaaggtgaa gcgtaagaat ccgccactag cgcgcctcga
     1981 tgatggcggg ctcgttgcgg ccttgcgcca gctcgaagcg aagcttggcg ctgccggcca
     2041 tgccacgcgc ctcggcgagc agtctttggc cctccgcctc cgatggcgcg aaggcaaagc
     2101 ccgtcggacc ccatgagctt tgcccgagcc cggtaacgcc gtaagccgcg aggccgttga
     2161 gcacagtggc aacgccgtcg ctgacatagg cgccgccctg catcggcgcg aaataggcgc
     2221 ccatcttttt ctgcagatag ccaacctgat cgcagaacgc cttgaagtcc tcctcggcaa
     2281 gcgccggtaa cgcgtcgagc agaacgcgcc tggtcaggtc agcggtctcg ctttccggga
     2341 aatccggcag cgtggcaaag gccgcggcct cctctgcgcc gtgcacgccg gtctccgccg
     2401 gatcgaagat caacagcacg cgccaggcgc gcgggaaagg cacgcgggcg agtaccggcg
     2461 gcaatttgcc tgggccagga cctccgtcga gcaccgcgcc gcctagccca aaggtgccga
     2521 tgccgatgcc cgagcgcttg ccccggtcga gccgcgcggc gatctcgtcg aaatcgagcg
     2581 gcaagccttc gagtgtggcg acagccgacc cgatggcaac cgcaagctgc gtgccggagc
     2641 cgaggcctgc atggccaggg atcacctcgt cgagatgcaa atgataggag gcggcgagac
     2701 ggaagcttgc cgcgatcgag cggagatagg gctcggcccg ctcgcaagga acgccggtga
     2761 ccttgagttc gctgccgcgc cgcaagctca
//
LOCUS       DATNAE010000114         2749 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3123334, whole genome shotgun sequence.
ACCESSION   DATNAE010000114 DATNAE010000000
VERSION     DATNAE010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3123334"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..400
                     /locus_tag="VN518_02475"
     CDS             188..400
                     /locus_tag="VN518_02475"
                     /inference="COORDINATES: protein motif:HMM:NF022894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin MazE family protein"
                     /protein_id="HXE03469.1"
                     /translation="MPNKRVRERVQSYRERMRARGLRPVQIWVPDTRTAKFRLEAHRQ
                     SLAVARSPTEHEDQSFIDAVSDLSEA"
     gene            397..714
                     /locus_tag="VN518_02480"
                     /pseudo
     CDS             397..714
                     /locus_tag="VN518_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019965555.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
     gene            complement(743..>2749)
                     /gene="lon"
                     /locus_tag="VN518_02485"
     CDS             complement(743..>2749)
                     /gene="lon"
                     /locus_tag="VN518_02485"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HXE03470.1"
                     /translation="LTGYPFLVALVDEVGEAEVYSTEIAARMHQLKERAREAISLLPA
                     IPKELADAIDQIQSPSALADFVANASDLTPAEKQELLETFDVQERLDKLLRILAQQIE
                     VLRLSKQIGEQTQESLQGRQREHILREQLRQIQKELGEGEGTGAEIAELREAIEKAKM
                     PEEAEKQALKELARLERTPEASAEHSMIRSYLDWLIELPWSKLSDERIDIAEARRILD
                     EDHYGLPKVKQRILEFLAVRKLNPEGKSPILCFVGPPGVGKTSLGQSIARAIGRKFVR
                     LSLGGLHDEAEIRGHRRTYIGALPGNVIQAIRKAETRNPVMMLDEMDKLGQGFHGDPS
                     AALLEVLDPEQNSTFRDNYLAVPFDLSRVMFIGTANVIDTIPGPLRDRMEIIELPGYT
                     EEEKVEIAKRYLLKRQLKATGLSPGACEITDDAIRAIIQDYTREAGVRNLEREIGAIC
                     RHVATRVAEGKVEHMKIDAADLHAILGARRFESEVAMRTSVPGVSTGLAWTPTGGDIL
                     FVECARVPGNGKLILTGQLGDVMKESAQAALSLVKARSASLGVDASVFEKSDIHLHVP
                     AGAIPKDGPSAGVAMFIAIVSLLTGRTVKNDTAMTGEISLRGLVLPIGGVKNKVLAAM
                     RAGITTVLLPERNKKDFEDVPEAARQAMKFVWLSTVDDAIAAAF"
BASE COUNT          473 a          842 c          898 g          536 t
ORIGIN      
        1 ctcaatgcct atttcgagac ctaccgctca ggcgatgcgt acgatcgcaa cgccatcacc
       61 cacgtcatgg cgtgccgctc gcattttccg gggctgctgc tgaggtcgtg atgaggtcag
      121 cttgcattct gggatttacg agtgtacgtt acatgtaact tgttgctaga cagcggcggt
      181 cgaaaaaatg cctaacaagc gagttcgaga gagggtgcag agctaccggg agcgtatgcg
      241 cgcgaggggc ctgcgacccg tgcaaatttg ggttcctgac acgcggaccg caaagttccg
      301 ccttgaggcg catcgacaat cgcttgccgt ggcgcgcagt ccgactgagc acgaggatca
      361 atcgtttatc gacgcggttt cggacctcag cgaggcatga aacgaggcga ggtttggacg
      421 attgcgggcg agggttatgc cgggaagcct cgccccgctg ttatcgtcca agatgaccgc
      481 tttgacgcaa ccgcgtcgat cacaatctgt gcctttacga ccgatctcac cgacgcgccg
      541 ctattccgtt tgccggtcgc accatccgag cgcaatgggc tgcgaagcgc ctctcgcctg
      601 atggtggata agatcactac cgtttcccaa agcaagttgg gaaaactgat aggccgactc
      661 gaccaagagg accttgtgcg gttggaccgc gccatcatgg ttttccttgg ggtttgcttg
      721 acgcgcgcct gcgctcgcct gactagaacg cagcagcgat ggcgtcgtcg acagtcgaga
      781 gccacacgaa cttcatcgcc tggcgcgccg cttccggcac gtcctcgaag tccttcttat
      841 tgcgctcggg cagaagcacg gtggtgatgc ctgcgcgcat ggcggcgagc accttgttct
      901 tgacgccgcc aatgggcagc accaagccgc gcagcgagat ctctccggtc atggcggtat
      961 cgttcttcac cgtacgcccg gtgaggagcg acacgatggc gatgaacatg gcgacgccag
     1021 cgctcgggcc gtccttaggg atggcgccag ccggcacatg caaatgaata tccgacttct
     1081 cgaacacaga cgcgtcgacg ccgagcgagg cgcttcgcgc ctttaccagg cttagggccg
     1141 cttgggcgct ttccttcatg acgtcgccga gctggccggt gaggatcagc ttgccgttgc
     1201 cgggcacacg tgcgcattcg acgaacaaaa tgtcgccgcc ggtcggggtc caggcaagcc
     1261 cggtcgacac gccgggcacg ctcgtgcgca tggcgacttc gctctcgaag cggcgggcgc
     1321 cgaggatggc atgcagatcg gcggcatcga tcttcatgtg ctccaccttg ccttcggcca
     1381 cgcgggtggc gacgtggcgg cagatggcgc cgatctcgcg ctcgagattg cgcacgccgg
     1441 cctcgcgcgt gtagtcctga atgatggcgc ggatggcgtc gtcggtgatc tcgcaagcgc
     1501 caggcgacaa gccggtagcc ttgagctgac gcttgagcag atagcgcttg gcgatctcga
     1561 ccttctcctc ctcggtgtag ccggggagct cgatgatctc catgcggtcg cggagaggcc
     1621 cagggatggt gtcgatcacg ttggccgtgc cgatgaacat cacccgcgac aggtcgaagg
     1681 ggaccgcgag ataattgtcg cggaaggtcg agttctgctc ggggtcgagc acctcgagca
     1741 gagccgccga cgggtcgccg tggaagccct ggccgagctt gtccatctcg tcgagcatca
     1801 tcaccggatt gcgcgtctcg gctttgcgga tcgcctggat cacgttgccg ggcagcgcgc
     1861 cgatataggt gcggcgatgg ccgcggatct ccgcctcgtc atgcaggccg ccaaggctca
     1921 ggcgcacgaa tttgcggccg atggcgcgcg cgatcgattg gccgagcgag gtcttgccca
     1981 cgccgggagg accgacgaag cagaggatgg ggctcttgcc ttcggggttg agcttgcgca
     2041 cggcgagaaa ttcgaggatg cgctgcttga ctttgggcag gccgtaatgg tcctcgtcga
     2101 ggatccggcg cgcttcggcg atgtcgatgc gctcgtcgga cagcttcgac cacggcaact
     2161 cgattagcca gtcgaggtag ctgcggatca tggagtgctc ggcgctcgcc tcaggcgtgc
     2221 gctcaagccg cgccagctcc ttcagcgcct gcttctcggc ctcctccggc atcttggcct
     2281 tctcaatggc ctcacgcagt tccgcgatct cggcgccggt gccttcgccc tcgccaagct
     2341 ccttctgaat ctggcgaagc tgctcgcgca agatgtgctc gcgctgccgg ccttgcagcg
     2401 attcctgggt ctgctcgccg atctgcttgg acaggcgcaa gacctcgatc tgctgcgcga
     2461 ggatgcgcaa cagcttgtcc aggcgctcct ggacatcgaa ggtctcgagc agctcctgct
     2521 tctcggcagg cgtgagatct gaggcgttgg cgacgaagtc ggcaagcgcc gagggcgact
     2581 gaatctgatc gatggcgtcg gcgagctcct tggggattgc cggcagcagc gatatcgcct
     2641 cgcgtgcccg ctctttcagc tgatgcatgc gggcggcgat ctcggtcgaa tagacctcgg
     2701 cctcgcccac ctcatcgaca agggcgacga ggaagggata accggtgag
//
LOCUS       DATNAE010000115         2780 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2220042, whole genome shotgun sequence.
ACCESSION   DATNAE010000115 DATNAE010000000
VERSION     DATNAE010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2220042"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="VN518_02490"
     CDS             <1..607
                     /locus_tag="VN518_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HXE03471.1"
                     /translation="EPYRMFTSRAEYRLTLRADNADQRLTPLGIALGCVGAERASAFD
                     AKAKALADGEALLKRLSLTPDEAGKRGLTVNRDGRKRSAYELLSYPDIDLATLAPIWP
                     EIGALDAKIAEQLAVDARYAVYLKRQEIDIAAFRKEESVTIPFGFSFDVIAGLSTELR
                     QKLERDRPASLGQAARLDGMTPAALLLLLAHLKKGSRWKTA"
     gene            592..1284
                     /gene="rsmG"
                     /locus_tag="VN518_02495"
     CDS             592..1284
                     /gene="rsmG"
                     /locus_tag="VN518_02495"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HXE03472.1"
                     /translation="MEDRLTATGARRSNPISGPEQFAEAFKVPRETIHRLIRYAELLT
                     EGQTRANLVSTKTLPQLWDRHFADSAQILRLAPDAKVWLDLGSGAGFPGLVIAILQAN
                     HANFRMHLVESTAKKCAFLAEVARATSAPVEIHCMRIEELSKTATSLKPEIITARAVA
                     PLPRLLELAAPWFGPGVRGLFMKGRDAGKEIESAKRRFNFTCHPHPSMTAEDSFILEI
                     GDLAKRAKAKTR"
     gene            1281..2093
                     /locus_tag="VN518_02500"
     CDS             1281..2093
                     /locus_tag="VN518_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HXE03473.1"
                     /translation="MSPTGPRRTRVIALANQKGGVGKTTTAINLGTALAAIGERVMIV
                     DLDPQGNASTGLGIDERHHSTSDVLTGKVPFAAARVETNVPGLSVVPATIDLMAFERE
                     SMSTSGRHYRLRDAIALMLAAEDPDQTTTYILIDCPPSLNLLTVSALAAADAVLVPLQ
                     CEFFALEGLAQLLSTVEEIRVRLNPKLLIHGVVLTMYDQRTTLADQVVDDVRRVLGDK
                     VYATIIPRNVRVAESPSHGKPVLLYDYRCSGSQAYIRLASEVIERERRMRAA"
     gene            2134..>2780
                     /locus_tag="VN518_02505"
     CDS             2134..>2780
                     /locus_tag="VN518_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HXE03474.1"
                     /translation="MAVGMHKMRLGRSLASLIGEGEGSEFGEMEGQRLVALDTLKAGR
                     FNPRRNFSEAQIEELAVSIRERGLVQPLVVRPVRGEHESYEIVAGERRWRAAQRANLH
                     EVPVVVRALSDKEAIEIAIIENVQREDLNAVEEAEGYRLLMDGHGYTQEDLSKVIGKS
                     RSHLANTLRLLKLPESVQALLRSGALSAGHARCLIGRADAAALAERIVNEGLTVR"
BASE COUNT          509 a          927 c          880 g          464 t
ORIGIN      
        1 cgagccctat cgcatgttca ccagccgcgc cgagtatcgc ctcacgctcc gcgccgacaa
       61 cgccgaccag cgcctcacgc cgttgggtat cgctcttggc tgcgtcggag ccgagcgcgc
      121 gtcagccttc gatgcgaagg cgaaagcgct cgcagatggt gaagctctgc tcaagcgcct
      181 ctcgctcacg cccgacgaag ctggcaagcg cggcctcacc gtcaatcgcg acggccgcaa
      241 gcgcagcgcc tatgagctgc tctcctatcc cgacatcgac ctcgcaacgc tcgctcccat
      301 ctggcccgag atcggcgcac tcgatgcaaa gatcgccgag cagctcgcgg tcgatgcgcg
      361 ctatgcggtc tacctcaagc gccaggagat cgacatcgcc gccttccgca aggaggagag
      421 cgtcaccatc ccgttcggat tttccttcga cgtgatcgca ggtctctcca ccgagctcag
      481 gcagaagctc gagcgcgatc ggccggcaag cctcggccag gccgctcggc tcgacggcat
      541 gacgccagcg gcactcctcc tgctgcttgc gcatctgaag aagggctcgc ggtggaagac
      601 cgcctgaccg cgacgggcgc ccgtcggtca aatcccatca gcgggcctga gcagtttgcc
      661 gaagccttta aagttccacg tgaaacaatc cacaggctta ttcgctacgc ggagcttctg
      721 accgaagggc agacacgtgc aaatctggtc tcgaccaaga ccctgcccca gctttgggac
      781 cggcatttcg ccgattccgc ccaaattcta cgcctggccc ccgacgccaa ggtctggctc
      841 gacctcggct cgggcgcggg cttccctggc ctcgtcattg ccatcttgca ggcgaatcac
      901 gctaatttcc gcatgcatct ggtggagagc accgccaaga agtgcgcgtt ccttgccgag
      961 gtcgcccgcg ccacatcggc gcctgtggag atccactgca tgcgcattga agagctaagc
     1021 aagacggcta caagcctcaa gcccgagatc attaccgcgc gcgccgtcgc gccattgccg
     1081 cgactgctcg agctggcggc gccgtggttc gggccagggg tgagggggct tttcatgaag
     1141 ggccgcgacg ctgggaagga aatcgagtcc gccaagcggc ggttcaactt cacctgccat
     1201 ccgcatccaa gcatgaccgc ggaggactcc ttcatcttgg agatcggcga tctcgccaag
     1261 cgcgcaaagg cgaaaacgcg atgagcccga ccggcccgcg ccgcacccgc gtgatcgcgc
     1321 tcgccaatca gaaaggcggc gtcggcaaaa ccaccacggc aatcaatctc ggcaccgcgc
     1381 ttgcggcgat cggcgagcgg gtgatgatcg tcgacctcga cccgcaagga aatgcgagca
     1441 ccggtctcgg catcgatgag cgccaccatt cgacctcgga cgtgctgacc ggcaaggtgc
     1501 ctttcgccgc ggcgcgcgtt gagaccaatg tgcccggcct ctcggtggtg ccggcgacga
     1561 tcgacctcat ggccttcgag cgcgagtcga tgagcacctc aggccggcac tatcgcttac
     1621 gcgacgccat cgccctgatg ctcgccgccg aagaccccga ccagaccacg acctacatcc
     1681 tcatcgattg cccgccgtcg ctcaatctcc tcaccgtgag cgcactcgcc gccgccgacg
     1741 ccgtgctcgt gccgctgcaa tgcgaattct tcgctttgga aggcctggcg caactcttga
     1801 gcaccgtgga agagatcagg gtgcgcctca atccgaagct gcttatccac ggggtggtgc
     1861 tcaccatgta cgatcagcgc acgacgctgg ccgatcaggt ggtcgacgac gtgcgccgcg
     1921 tgctcggcga caaggtctac gccaccatca tcccgcgcaa tgtgcgcgtg gctgagtcgc
     1981 cgtcgcatgg gaagccggtg cttctctacg actatcgatg ctcaggcagc caggcctata
     2041 tcaggctcgc ttccgaggtg atcgagcgcg agcggcggat gcgtgcggcg taagttgcgt
     2101 cggaaaagcg gcggcggaaa caaggaggcc atcatggctg tcggcatgca caagatgagg
     2161 ctcgggcgaa gccttgcctc gctcatcggc gagggtgagg gctccgagtt tggcgagatg
     2221 gaaggccagc gcctcgtggc gctggatacg ctcaaagccg gccgcttcaa tcccaggcgg
     2281 aatttctcgg aagctcagat cgaggagctt gcggtgtcga tccgcgagcg ggggctcgtg
     2341 cagccgctcg tggtgcggcc cgtccgaggc gagcatgagt cgtatgagat cgtcgccggc
     2401 gagcggcgct ggcgggcggc gcagcgcgcc aatctgcacg aggtgccggt ggtggtgcgc
     2461 gcgctctccg acaaggaagc gatcgagatc gccatcatcg agaacgttca gcgcgaagac
     2521 ctgaacgcgg tcgaggaggc cgaaggctat cggctgttga tggacggtca cggctatacg
     2581 caggaagacc tgtccaaggt gatcggcaag agccggagcc atctcgccaa cacgctccgc
     2641 ctgctcaagc tgcctgagag cgtgcaagct ttgttgcggt ctggcgcgct cagcgccggg
     2701 cacgcgcgct gcctgatcgg gcgcgccgac gccgcggccc ttgccgagcg gatcgttaac
     2761 gaagggttaa cggtgcgcca
//
LOCUS       DATNAE010000116         2818 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3724961, whole genome shotgun sequence.
ACCESSION   DATNAE010000116 DATNAE010000000
VERSION     DATNAE010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3724961"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /gene="eno"
                     /locus_tag="VN518_02510"
     CDS             complement(<1..743)
                     /gene="eno"
                     /locus_tag="VN518_02510"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012707838.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HXE03475.1"
                     /translation="MSTIIDITGRQIFDSRGNPTVEVDVVLDDGSFGRAAVPSGASTG
                     AHEAVELRDGGKEFLGKGVTKAVDFVNGEIFDEVQGLDALDQIGLDRAMIELDGTPNK
                     GRLGANAILGVSLAAAKAAAESSELPLFRYVGGPNAHVLPVPMMNIINGGAHADNPID
                     MQEFMILPVGAESMAHAVQIGSEIFHTLRKNLAADGHNTNVGDEGGFAPNLKSAEDAL
                     AYMVKSIKKAGYTPGEDVCLGLDCASTEY"
     gene            964..>2818
                     /gene="topA"
                     /locus_tag="VN518_02515"
     CDS             964..>2818
                     /gene="topA"
                     /locus_tag="VN518_02515"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014517394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HXE03476.1"
                     /translation="MKLVIVESPAKAKTINKYLGKDYEVLASFGHVRDLPAKDGSVRP
                     DEDFAMSWEVDASSRKHVADLVSAAKDADELILASDPDREGEAIAWHVRDVLEQKKAI
                     KGKPVRRVVFNAITKSAVTEAMLHPREIDAPLVDAYLARRALDYLVGFTLSPILWRKL
                     PGSRSAGRVQSVALRLVTEREAEIEKFKAQEYWSVLAHLLHRGKGFDARLYAVDGNVS
                     DKLDVKTGSEAEALKKAIDDGRLYVTTVDKKPTKRNPYAPFTTSSLQQDASSRLGFSP
                     SRTMQIAQRLYEDGVITYMRTDAVQMAPEAINATRQAIEKEFGAAYLPPTPRLYQTKA
                     KNAQEAHEAIRPTDLLRHPNTLRGGDNDQHRLYELIWRRTMASQMKAAEIERTTADIL
                     VEGSPRNVTLRAVGSVVTFPGFLALYGVEAKEDRGGDDEDEDSRELPPLVAGDRPDLK
                     DVEIEQHFTQPPPRFTEASLIKRMEELGIGRPSTYASTLSVLRDRNYVRLEKRALVPE
                     DRGRLVTAFLESFFKRYIEYGFTADLEEKLDEISDGKLDYKEVLRAFWDEFSRHTTEI
                     KDLRVTEVLDALNDLLADHIFPAKADGSDPRRCPNCGTGQLSLKLGKFGAFI"
BASE COUNT          519 a          926 c          890 g          483 t
ORIGIN      
        1 atatattcgg tcgaggcgca atcgaggccg aggcagacat cctcgcccgg cgtgtagccg
       61 gcctttttga tcgacttgac catataggcg agcgcgtctt cggcggattt gaggttcggc
      121 gcaaagccgc cctcgtcgcc gacattggtg ttgtggccgt cggcggcgag attcttccgg
      181 agcgtatgga agatttccga gccgatctgg acggcatgcg ccatgctctc ggcgccgacc
      241 ggcaggatca tgaactcctg catgtcgatc gggttgtccg cgtgagcgcc gccgttgatg
      301 atgttcatca tcggcaccgg caggacgtgg gcgttggggc cgccgacgta gcggaatagc
      361 ggcagctcgg aggattccgc ggctgccttg gccgccgcaa gcgagacgcc gagaatggcg
      421 ttggcgccga gccggccctt gttcggcgta ccgtcgagct cgatcatggc gcggtcgagg
      481 ccgatctggt cgagcgcatc gaggccctgc acctcgtcga agatttcgcc gttgacgaaa
      541 tccaccgcct tggtgacgcc cttgcccaaa aactccttgc cgccgtcgcg cagctcgacg
      601 gcctcgtgtg cgccggtcga ggcgcccgac ggcaccgccg cgcggccgaa actgccgtcg
      661 tcgagcacca catccacctc gaccgtcgga ttgccgcggc tgtcgaaaat ctgccggccg
      721 gtgatgtcga tgatcgtgga catgtgtcgc cttcttgctt gtctctacga ggaaggccct
      781 ctctaggccg cacatatgtc aactggaagg gggtgcggcg aaaaatccgg cgcttcgcct
      841 gttcgcggcg gcggcttctc ccctacgggg agggtcttgc gaccaccgct ggcgagttga
      901 cacggcaaat cggcttcacc atgttgcgcc gcgttcgcca agccgttttt gaaagccttt
      961 cgcatgaagc tcgtgatcgt tgaaagtccg gctaaggcca agacaatcaa caagtatttg
     1021 ggcaaggact acgaggtcct ggcgagcttc gggcatgtcc gcgatttgcc cgccaaggat
     1081 ggctcggtcc gccccgacga agacttcgcg atgagctggg aggtcgatgc ctcctcccgc
     1141 aagcacgtcg ccgatctcgt cagcgccgcc aaggacgccg acgaactgat cctcgcatcg
     1201 gacccagatc gcgagggcga agcgatcgcc tggcatgtgc gggacgtgct ggaacagaag
     1261 aaggcgatca agggcaagcc ggtccgccgc gtcgtcttca atgccatcac caaatcggcg
     1321 gtgaccgagg cgatgctgca tccgcgcgaa atcgatgcgc cgctggtgga cgcctacctg
     1381 gcgcgccggg ccctcgacta tctcgtcggc ttcaccctgt cgccgatcct ctggcgcaag
     1441 ctccccggct ctcgttcggc cggccgggtg cagtcggttg cgctgcgcct cgtcaccgaa
     1501 cgcgaagccg agatcgaaaa gttcaaggcg caggaatact ggagcgtcct tgcgcacctg
     1561 ctgcaccgcg gcaagggctt tgacgcccgg ctctatgcgg ttgacggcaa cgtatccgac
     1621 aagctcgacg ttaagaccgg aagcgaagcc gaggccctca agaaggcgat cgacgacggc
     1681 cggctctatg tgacgaccgt cgacaagaag ccgaccaagc gcaaccccta cgcgccgttc
     1741 accacgtcga gcctgcagca ggacgcttcc tcgcgcctcg gcttttcgcc gagccgcacc
     1801 atgcagatcg cgcagcgcct ctacgaggac ggcgtcatca cctatatgcg aaccgacgcc
     1861 gtgcagatgg cgcctgaggc gatcaacgcc acccggcagg ccatcgagaa ggagttcggc
     1921 gccgcctacc tgccgccgac accccgcctc taccagacca aggccaagaa tgcgcaggag
     1981 gcacacgagg cgatccggcc gaccgatctg ctgcggcacc ccaatacgct gcgcggcggg
     2041 gacaacgacc agcaccgcct ctacgagctg atctggcggc gcaccatggc gagccagatg
     2101 aaagcggccg agatcgagcg cacaacggcc gatatcctcg tcgaaggctc gccccgcaac
     2161 gtgacgctcc gggccgtcgg ctccgtggtg acgttccctg gatttctcgc gctctatggc
     2221 gtcgaggcca aggaagatcg gggcggcgat gatgaggacg aagactcgcg tgagctgccg
     2281 ccgctggtgg caggagatcg ccccgatctc aaggacgtcg agatcgagca gcacttcacc
     2341 cagccgccgc cgcgcttcac cgaagcgagc ctgatcaagc ggatggaaga gctcggcatc
     2401 ggccgcccgt cgacctatgc ctcgacgctc tcggtgctca gggaccgcaa ctatgtccgg
     2461 ctggaaaagc gggcgctggt gcccgaggat cgcggccggc tggtcacggc gtttctcgag
     2521 agcttcttca agcgctacat cgaatatggc ttcaccgccg acctcgaaga aaagctcgac
     2581 gaaatctcgg acggcaagct cgactacaag gaggtgctgc gcgccttctg ggacgagttc
     2641 tcgcggcata cgaccgagat caaggatctg cgcgtcaccg aggtgctgga tgcgctcaac
     2701 gatctccttg ccgaccacat ctttccggca aaggccgatg gctccgatcc gcgccgctgc
     2761 cccaattgcg gcaccggcca actgtcgctc aagctcggca agttcggcgc cttcatcg
//
LOCUS       DATNAE010000117         2748 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2827415, whole genome shotgun sequence.
ACCESSION   DATNAE010000117 DATNAE010000000
VERSION     DATNAE010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2827415"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="VN518_02520"
     CDS             complement(<1..469)
                     /locus_tag="VN518_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086546.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HXE03477.1"
                     /translation="MQRFLRRVAVIGGARIPFCRSNTFYADLSNLDLMTGALNALVDR
                     YNLKGQHIDEVVGGAVITHSKDFNLTREALLGTRLKASSPGITMMQACGTSLQGGLGS
                     AAKIATGEIECAIAVGSDTTSDAPLAVSKKLAQRLVKLGQQKTFMDKLKLFKGF"
     gene            complement(494..2500)
                     /locus_tag="VN518_02525"
     CDS             complement(494..2500)
                     /locus_tag="VN518_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HXE03478.1"
                     /translation="MFKALSRPLPDLKDWRFSVDQWGIGWAEFDREGENQNSLGRRPL
                     EELAQIVAKVEEGARDKTIRGLVVISAKERGFIVGADIREFADLTTESEVIEKLRSVT
                     DLFDRIERLPVPVVCAINGFCLGGGLELALACHYRIATRDSGTKLGFPEVKLGIFPGF
                     NGTARSIRLAGAPAAMQAMLTGSMLSASAARGVGLVDQLVDSRGALPWAARKAVLRNR
                     KSKPAGFVKQLLSKWPARDLLAKRMRQEVGKKAREDHYPAPYRLIDLFERYGGNDEAM
                     KAAETRAFAPLMVSDQSRNLRRVFWLSELLKGQAPKKLAWRPSRVHVIGAGTMGADIA
                     GWCVARGMEVTLQDVSLEQIAKGIAAQGKLFARKFKTKALRDAAKARLIADPKGDYIH
                     HADVVIEAIVENLEIKQKVLGDVERKMKPGAVLATNTSSLLIEDIAAGLPDPGRLIGL
                     HFFNPVALMPLVEVVRGPQTREEDVKRGAAFVAAIDKFPLITKSVPGFLVNRVLTPYM
                     FGAMQRLEHGEDKERIDEAARTFGMPMGPIELADTVGLDVCAHVAHILKYSSEGSKLD
                     RLVAEGKLGKKTGEGFYVWKDGKPEKTGRNFDKADMERLGRELVEPLIAEAQKSVDEG
                     VVENADLVDAGVIFGTGFAPFRGGPLHYKAAQTPATPTQAAAAE"
     gene            complement(2565..>2748)
                     /locus_tag="VN518_02530"
     CDS             complement(2565..>2748)
                     /locus_tag="VN518_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03479.1"
                     /translation="AMAQGIVTESEGQQLRELEALTARVIAVDHFDPAALRPNYKTNA
                     GPVGHNSRGIESAAPE"
BASE COUNT          445 a          890 c          916 g          497 t
ORIGIN      
        1 agaagccctt gaacagcttc agcttgtcca taaacgtctt ctgctggccg agcttgacca
       61 gacgttgcgc cagcttcttc gacaccgcga gcggcgcgtc tgacgtcgta tcggagccga
      121 cggcgatggc gcattcgatc tcgccggtag caatcttggc ggcgctgccg aggccgccct
      181 gcaggctggt gccacatgcc tgcatcatcg tgatgccggg agacgatgcc ttgaggcgcg
      241 tcccgagcag cgcctcgcgc gtgaggttga aatccttcga gtgcgtgatg acggcgccgc
      301 cgaccacctc gtcgatgtgc tggcctttga ggttgtagcg atccacgagg gcgttgagcg
      361 caccggtcat cagatcgagg ttggagaggt cggcatagaa ggtgttcgag cggcagaagg
      421 gaatgcgtgc tccgccgatc accgcgacgc gtctcaagaa acgttgcatc tcgtctgcct
      481 cttgcttcga gtgttattcg gcggcggcgg cttgcgtggg ggtggccggt gtctgcgccg
      541 ccttgtagtg caagggtcca ccgcggaacg gcgcaaaacc ggtgccgaag atgacgccgg
      601 catcgacaag gtcggcgttc tcgacaacgc cctcgtcgac actcttctgc gcctcggcga
      661 taagcggctc gaccagctcg cgcccgagcc gctccatgtc cgccttgtcg aagttgcggc
      721 cagtcttttc cggtttgccg tccttccaca cgtagaagcc ttcgcccgtc ttcttgccga
      781 gcttgccctc ggccacgagg cgatcaagct tagaaccttc cgaggaatac ttgaggatgt
      841 gggcgacgtg ggcgcagacg tcgagtccga cggtgtcggc gagctcgatg gggcccatgg
      901 gcattccgaa agtgcgcgcc gcctcgtcga tgcgctcctt gtcctcgcca tgctcgaggc
      961 gctgcatggc gccgaacatg tagggcgtca gcacgcggtt gacgaggaag ccgggcacgc
     1021 tcttcgttat cagcgggaac ttgtcgatgg cggcgacgaa ggcggcgccg cgcttcacat
     1081 cttcctcgcg cgtctgcggg ccacgcacca cctcgaccaa cggcatgagc gctacgggat
     1141 tgaagaagtg caggccgatg aggcgcccgg ggtcgggcag accggcagcg atgtcctcaa
     1201 tcagcagcga cgaggtattg gtcgccagca cggcgccagg cttcatcttc ctctcgacgt
     1261 cgcccaggac cttctgcttg atctcgaggt tctcgacgat cgcctcgatg acgacgtcgg
     1321 cgtggtggat atagtccccc ttgggatcgg cgatgagccg cgccttggcc gcatcgcgca
     1381 gggccttggt cttgaacttg cgcgcgaaca acttgccctg cgcggcgatg cccttggcga
     1441 tctgctcgag cgagacgtct tgcagcgtca cctccatgcc gcgggcgacg caccagccgg
     1501 cgatgtcggc gcccatggtg cctgcgccaa tgacgtgcac gcggctggga cgccaggcta
     1561 gctttttcgg tgcctgaccc ttgagcaact ccgacagcca gaagacgcgg cgcaggttgc
     1621 gcgattggtc cgacaccatg agcggcgcga aggcgcgcgt ctcggcggcc ttcatcgcct
     1681 cgtcgttgcc gccgtagcgc tcgaagagat cgatgagccg gtaaggcgcg gggtagtgat
     1741 cctcgcgcgc cttcttgccc acctcctggc gcatgcgctt ggcgaggaga tcgcgcgccg
     1801 gccacttgga caggagctgc ttgacgaacc cggccggctt cgacttgcga ttgcgcaaga
     1861 ctgccttgcg tgccgcccag ggaagcgcgc cgcggctgtc gacgagctga tcgacgaggc
     1921 ccaccccgcg cgccgccgaa gccgacaaca ttgatccggt caacatcgcc tgcatggcag
     1981 ccggagcgcc ggcgaggcgg atggaccgcg ccgtgccgtt gaagcccgga aagatgccga
     2041 gcttcacttc cgggaagccg agcttggtgc cggaatcgcg cgtcgcaatg cggtagtggc
     2101 acgccagcgc cagctcgagg ccgccgccga ggcagaagcc gttgatggcg cagacgaccg
     2161 gcacgggcag gcgctcgatg cgatcgaaca gatccgtgac tgagcgcagc ttctcgatga
     2221 cctcggattc ggtggtgagg tcggcgaact cgcgaatgtc ggcgccgacg atgaagccgc
     2281 gctccttggc cgagatgacg acgaggccgc ggatggtctt gtcgcgcgca ccctcctcca
     2341 ccttggcgac gatctgtgcc aactcctcga gcggacggcg cccgagcgag ttctggtttt
     2401 caccctcgcg atcgaactcg gcccagccga tgccccactg atcgacggag aaacgccagt
     2461 ccttgaggtc gggcagcgga cgcgacaacg ccttgaacat cgcaactcct ctcgacgcaa
     2521 gcggcggcgc ctaggggagg cgccggacca ccgtgcggtc ttttctactc gggcgcggcg
     2581 ctctcgatgc cgcgcgaatt gtgccccacc gggccggcgt tcgtcttgta gttgggccgc
     2641 agcgcggcag gatcgaagtg atcgacggcg atgacgcggg cggtgagcgc ttccaactcg
     2701 cgcagctgct ggccttcgct ttcggtcacg atgccctgtg ccatcgct
//
LOCUS       DATNAE010000118         2803 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3728223, whole genome shotgun sequence.
ACCESSION   DATNAE010000118 DATNAE010000000
VERSION     DATNAE010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3728223"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..1437)
                     /locus_tag="VN518_02535"
     CDS             complement(91..1437)
                     /locus_tag="VN518_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011424371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03480.1"
                     /translation="MTIRPGRLVLTAAIALLWGCTPSSNAPMANAPAAGADAGFDATI
                     DSNSNALLTEGRKIFRYDTFGSEAFWGDQLHLHQAIAGEKNGGVGPGVSPKTALSLGL
                     KVDAAALPSAVVDAIKAGKVDLDDPATTVTLLKLNAVVGVTGFFDASGKLTSMGIQCA
                     FCHSTVDNSFAQGIGKRLDGWPNRDLNVGGIVSAAPNLKPLTDYLGVDKATLLKVLAS
                     WGPGKYDAEINQDLKAMRPDGKSAATLIPAAFGLAGQNLHTYTGWGSVPYWNAYVAIT
                     QMHGQGTFFDPRLDNAKQFPLAARNHAGNIRPAKDLVTAKLAALQYYQLSIPAPVPSH
                     GSFDARAAEAGKAVFNGAAQCSTCHVPPLFTEPGWGMHTAAEIGIDDFQASRSPDKKF
                     YRTTPLRGLFVRAKGGFYHDGRFEDLKAVVAHYNRVLNLALTSAETGELIEYLKSL"
     gene            complement(1743..>2803)
                     /locus_tag="VN518_02540"
     CDS             complement(1743..>2803)
                     /locus_tag="VN518_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003525818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HXE03481.1"
                     /translation="VFFNNSGSEANDTAVKMVWYFNNARGRHRKKKIIARMKGYHGVT
                     VAAASLTGLANNHRDFDLPIANIRHADCPHHYRFAKSGESEEEFATRLADSLEAQILR
                     EDPETVAAFIAEPVMGAGGVILPPRTYFEKIQKVLGKYDVLLIADEVICGFGRTGNMF
                     GTETFGLKPDIMTMAKALSSAYLPISATIISEELYRAFVEQSEKIGTFAHGYTYSGHP
                     VPCAVALEALKIYEERDILAQVRRVSPRFMEGLRRFAAHPLVGEVRAIGLIGAAELVQ
                     DKASKAPFDPKQGVGAIFAAHALEGGLIVRAMGDSVALCPPLIITSDEIDEMLRRFAK
                     ALDDTAATVKRMGLASVA"
BASE COUNT          460 a          854 c          968 g          521 t
ORIGIN      
        1 ggggaggtcc agaagacaga caagccgggc gtgacgcgtg aaaagccggt tgccatcgct
       61 ctggagcctt cgtccatcct cgcccacgtg tcagagcgat tttaggtact cgatgagttc
      121 ccccgtctcc gcggacgtta acgctaagtt caacacgcga ttatagtgtg cgacgacggc
      181 cttcaggtcc tcgaagcggc catcatggta gaagccgcct ttggcgcgca cgaatagacc
      241 tctcagcggt gtcgtccggt agaacttctt gtccggcgaa cggctcgcct gaaaatcatc
      301 gatcccgatt tccgcggcgg tatgcatccc ccatcccggc tcggtgaaga gcggcggcac
      361 atgacaggtc gagcactgcg cagccccgtt gaagaccgcc ttgcccgctt ccgcagcgcg
      421 cgcgtcgaag cttccatgcg aaggtacagg cgccgggatc gagagctggt agtactgcaa
      481 cgccgccagc tttgcggtca ccaagtcctt cgcggggcgg atattgccgg catgattgcg
      541 cgcagcgagc gggaactgct tggcgttgtc gagccgcgga tcgaagaacg tgccctggcc
      601 atgcatctgg gtgatggcga cataggcgtt ccagtagggc acgctgcccc agccggtata
      661 ggtgtggagg ttctgcccgg cgaggccgaa ggcggcggga atgagcgtcg ccgccgattt
      721 gccgtcgggg cgcatcgcct tcagatcctg gttgatctcg gcgtcgtatt tgccgggtcc
      781 ccagctcgcc agcaccttga gcagcgtcgc cttgtcgacg ccgagatagt cggtgagcgg
      841 cttaagattg ggcgcggccg agacgatgcc gccgacattg aggtcgcgat tgggccagcc
      901 atcgaggcgc ttgccgatgc cctgggcgaa ggagttgtcg accgtcgaat ggcagaacgc
      961 gcattggatg cccatcgagg tgagcttgcc cgaggcatcg aagaagccgg tgacgccgac
     1021 gacggcgttg agcttcagga gcgtgacggt ggtggcggga tcgtcgaggt cgaccttgcc
     1081 ggccttgatg gcatcgacga cggccgaggg cagggccgcg gcgtcgacct tgaggccgag
     1141 cgagagggcc gtcttcgggc tgacacccgg accgacgccg ccattcttct cgccggcgat
     1201 ggcttgatgg agatggagct ggtcgcccca gaaggcttcg ctgccgaagg tgtcgtagcg
     1261 gaagatcttc cgcccttcgg tgaggagcgc attcgagttg ctgtcgatgg tggcatcgaa
     1321 gccggcatcg gcgcccgcgg ccggcgcgtt cgccatgggc gcgttcgagg acggcgtgca
     1381 gccccacagc aaggcgatgg cagcggtgag taccagtctt ccaggtctga tcgtcatcag
     1441 ctcccccgtt ggcggccttt gacgcgacct tctgtcgcgt cgccgctcaa gctgtcagat
     1501 gcgggggagt tgaaaatgtt cccggcgccc acacggcgcc gttgacaact tcgtgatggt
     1561 taacggcgcc cgccctcagg cgtatccggg tttgacctag acgtccgctg cacgactgtc
     1621 cgtgcgtcct tcgacaaaca acccggccgc gctgtgacgc gcggccgggt tgtttgtcga
     1681 aggacgcagg atgattttgc cgcgatcggc ccggctatgg aatgtaaggc gctacccgcc
     1741 cctcaggcca ccgaggcgag gcccattcgc ttcaccgtcg ccgcggtgtc gtcgagcgcc
     1801 ttggcgaagc ggcgcagcat ctcgtcgatt tcatctgacg tgatgatgag cggcgggcag
     1861 agcgccacgc tgtcgcccat ggcgcggacg atcaggccgc cctcgagcgc gtgcgcggcg
     1921 aagatggcgc cgacgccttg cttgggatcg aagggcgcct tgctcgcctt gtcctgcacc
     1981 agctcggcgg cgccgatgag gccgatggcg cgcacttcgc cgaccagcgg atgcgcggca
     2041 aagcggcgca ggccctccat gaagcgcggc gagacgcggc gcacttgcgc caggatgtcg
     2101 cgctcctcgt agatcttcag cgcctcgagc gcgaccgcgc aggggacggg atggccggaa
     2161 taggtgtagc catgcgcgaa agtgccgatc ttctcgctct gctcgacgaa ggcgcggtag
     2221 agctcctcgc tgatgatcgt cgccgagatc ggcagatatg ccgaggagag cgccttcgcc
     2281 atggtcatga tgtcgggctt gaggccgaag gtctcggtgc cgaacatgtt gccggtgcgc
     2341 ccgaagccgc agatgacctc gtcggcgatg aggagcacgt cgtatttccc caggaccttc
     2401 tggatcttct cgaaatatgt acggggcggc aggatgacgc cgccggcgcc catcaccggc
     2461 tcggcgatga aggcggcgac ggtctcggga tcctcgcgca ggatctgcgc ctcgaggctg
     2521 tcggcgagcc gtgtcgcgaa ctcttcctcg ctctcgcctg atttggcaaa gcggtagtga
     2581 tgcgggcagt cggcgtggcg gatattggcg atcgggaggt cgaagtcgcg gtggttgttg
     2641 gcgaggccgg tgaggctcgc cgccgcgacg gtgacgccgt gatagccctt catgcgcgcg
     2701 atgatcttct tcttgcggtg ccggccgcgc gcattgttga agtaccatac catcttgacc
     2761 gcggtgtcgt tggcctcgga gccggaattg ttgaagaaca cct
//
LOCUS       DATNAE010000119         4993 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_4032264, whole genome shotgun sequence.
ACCESSION   DATNAE010000119 DATNAE010000000
VERSION     DATNAE010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4993
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4032264"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="VN518_02545"
     CDS             <1..574
                     /locus_tag="VN518_02545"
                     /inference="COORDINATES: protein motif:HMM:NF017815.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglutaminase-like cysteine peptidase"
                     /protein_id="HXE03482.1"
                     /translation="TIQFEPQLAPQKAAFLQSFGNSLPPIGYVTFCREHEAECKPSGP
                     IVERMQLTAQKRADLEQVNRYVNQSVSPVTDLDLYGKVEVWTYPSGKGDCEDYVLLKR
                     RMLIERGWPESTLLITVVRDENNEGHAVLTARTDVGDLVLDNKRQQIVRWTDTPYTFV
                     KQQSERNPLVWSSLTPPNGDSSQTTASHSR"
     gene            complement(733..945)
                     /locus_tag="VN518_02550"
     CDS             complement(733..945)
                     /locus_tag="VN518_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019223304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3126 family protein"
                     /protein_id="HXE03483.1"
                     /translation="MTRDEIIKLERYLQRVFRLPALQVKQRPRKEDSAEVYVGDEFIG
                     VLFRDDDEGETAYQFQMAILDYDLGT"
     gene            complement(983..1825)
                     /gene="cysE"
                     /locus_tag="VN518_02555"
     CDS             complement(983..1825)
                     /gene="cysE"
                     /locus_tag="VN518_02555"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019966486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HXE03484.1"
                     /translation="MNKAFTAAKPQIKSYDPIWEQVRREAEETSADEPVLASLIYATI
                     LSEPTLEDAVCHTVAHRLQPSVDTGLLHKTFRQVLTEDPGLGDIFRADIMAVAKRDPA
                     CRRLIEPLFFFKGFVALQTHRFAHRLWQSGRKEFALYLQSQSSRVFAVDIHPAARLGK
                     GMMLDHATGIVIGETAVVGDNCSMLHGVTLGGTGNERDDRHPKIGCGVMMGSGAKILG
                     NIHIGDCVRVAAGSVVVQDVPPRRTVAGVPARDIGPAGCEEPALSMDQLVRDGTDGSA
                     SPCG"
     gene            complement(1890..2642)
                     /locus_tag="VN518_02560"
     CDS             complement(1890..2642)
                     /locus_tag="VN518_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXE03485.1"
                     /translation="MPSFQSDGVRIAYWDEGEGEPILLIHGFASNVATNWIDPGWVST
                     LTESGRRVIAFDNRGHGQSEKLYEPERYGAPIMAEDARRLLDHLGIERADVMGYSMGA
                     RISAFLVLNHPARVRSAILAGLGINMVRGMVGTGPLAKALEAKSIDEVTNDTARSFRA
                     FAEHTGGDLAALAACMRGPREKITPELLAGIRVPVLVAVGTEDVIGGSGAELAALIPG
                     AKLLDIKGRDHMKAVGDASFKRGVLDFLTQRV"
     gene            2716..2979
                     /locus_tag="VN518_02565"
     CDS             2716..2979
                     /locus_tag="VN518_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007010384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HXE03486.1"
                     /translation="MAQTLVPHFINDKGVDRIAIGVKEFQCMGATPPFDHPHVYLDMG
                     DEAQLVCPYCSTLFHYDPALKATESDPPSCVFVATAPPGAPEA"
     gene            2991..4166
                     /locus_tag="VN518_02570"
     CDS             2991..4166
                     /locus_tag="VN518_02570"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HXE03487.1"
                     /translation="MTKSGPHNRALIVGGGIGGLTTAVALARTGMSATVLERSAFADE
                     TGAGIQLGPNATRALAQLGVLDAIAATAFRPDVLRLFDGLSGTSLATVPLGQIAKERY
                     GAPYLTLHRADLHASLLAACRAECAIELRSGFDATEAETLAEPVIVRGDDGTQVEGSI
                     LIAADGLWSRLRDRIAPDADLRFSGATAWRALLPRGEVPAPFAAPEVGLWLGPRAHLV
                     HYPVRGGKDLNIVAVVEGGSAKQGWSRRAEPDLLLPSFQRWAQPAKTLLEAVETWRCW
                     SLFRLKPLPRWTDGRMALLGDAAHPVLPYLAQGAALAIEDAITLAASLKASGGDPAAA
                     FPRYQSLRMDRAARIQAQAARFGRMYHLRGPAAFARNFLLGRRHPESLLQSFDWLYG"
     gene            complement(4175..>4993)
                     /gene="nhaA"
                     /locus_tag="VN518_02575"
     CDS             complement(4175..>4993)
                     /gene="nhaA"
                     /locus_tag="VN518_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020091315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HXE03488.1"
                     /translation="HPENWRGWAVPTATDIAFALGVLALVGSRVPGSLKVFLTSLAII
                     DDLGAILIIAIFYASNLSIAALAVAAVFAAALYWLNRRGETRLLPYILLGVLLWTAML
                     FSGIHATLAGVVLAMLIPIDSAGDGRHSLLHSMERALSPWVAFLVLPIFAFANAGVAI
                     KWNMSVLSPLGLGIVGGLFLGKQIGILSAGYLAKKARIAVLPAGASWRQIYGVAVLCG
                     IGFTMSLFIGLLAFTDPETEAVLKLSVLVGSLLSALVGALVLLTVTPAKSTPAT"
BASE COUNT          911 a         1690 c         1530 g          862 t
ORIGIN      
        1 cacgattcaa ttcgagcctc agctcgcgcc gcaaaaggct gccttcctgc agtccttcgg
       61 caacagcctg ccgccgatcg gctacgtgac cttctgccgg gagcacgagg ccgagtgcaa
      121 gccgtcaggt ccgattgtcg agcgcatgca gctcaccgcg cagaagcgcg ccgacctcga
      181 gcaggtgaat cgctacgtca accagtccgt cagtccggtg accgacctcg atctctacgg
      241 caaggtcgaa gtctggacct atccctcggg caaaggcgat tgcgaggact atgtgctgct
      301 caagcgccgc atgctgatcg agcgtggctg gcctgagagc acgttgctca tcaccgtcgt
      361 ccgcgacgag aacaatgaag ggcacgccgt gctcacggcg cgcaccgacg tcggcgacct
      421 cgtcctcgac aacaagcgcc agcagatcgt gcgctggacc gacacgcctt acaccttcgt
      481 caagcagcag tcggagcgca atccgctggt ctggagctcg cttacgccgc cgaacggcga
      541 ctcctcgcag accacggcct cgcatagccg ctagccacga ggagcctcga tgttctggag
      601 ataagtttca ggcccggtga acatccccca ccatccccga cccaaccggg cctcacctag
      661 agccggcctc acttcgagcg ccggctcctt tttttgtatt gcgttaaagc gtgagggacg
      721 gctcgcgccc ggctaggtgc cgaggtcgta gtcgagaatt gccatctgaa actgataagc
      781 ggtctcgccc tcgtcgtcgt cgcggaatag gacgccgatg aactcgtcgc cgacatagac
      841 ctcggcggag tcttccttgc gtggccgctg cttcacttga agggccggca ggcggaacac
      901 ccgctgcagg taccgctcaa gcttgatgat ctcgtctcgc gtcacgcaca cctccgattg
      961 atggcagagc cttggaaatg ccttagccgc atgggcttgc gcttccgtcc gtcccgtcgc
     1021 gcacgagctg gtccatgctg agcgccggtt cctcgcagcc ggcggggccg atgtcgcgcg
     1081 ccggcacccc ggcgacggtc cgccgcggcg gcacgtcctg caccaccacg cttccggccg
     1141 cgacccgcac gcaatcgccg atatggatat tgccgaggat cttggcgcca gatcccatca
     1201 tcacgccgca gccgatcttg ggatggcggt cgtcgcgctc attgccagtg ccgccgagcg
     1261 tcacgccatg tagcatcgag caattgtcgc ccaccaccgc cgtctcgccg atgacgatgc
     1321 cggtcgcgtg gtcgagcatc atgcccttgc ccagccgcgc cgccgggtga atgtcgaccg
     1381 cgaacacgcg gctcgactgg ctttgcaggt aaagggcaaa ttccttgcgc cccgattgcc
     1441 aaagcctatg cgcgaagcga tgggtctgaa gcgccacgaa acccttgaag aagaacaacg
     1501 gctcgatgag acggcggcaa gccggatcgc gcttagccac ggccatgatg tcggcacgga
     1561 aaatgtcgcc gaggcccgga tcctccgtca gcacctggcg gaaggtcttg tgcaagagcc
     1621 cggtgtcgac ggaaggctgg agccggtggg ccacggtgtg gcagaccgcg tcctcaagcg
     1681 tcggctcgct caagatcgtg gcgtaaatca ggctcgccag caccggttcg tcggcgctcg
     1741 tctcttccgc ttcccggcgc acctgctccc atatcgggtc gtagctcttg atctgcggct
     1801 ttgccgcggt gaaggccttg ttcatgacaa ccacctctct gaagctccct tcattcgagg
     1861 gctaatttcc tgtttcacta gcacgggact taaacgcgct gggttaaaaa gtcgagcacg
     1921 ccccgcttga aactcgcgtc gccgaccgcc ttcatgtgat cgcggccctt gatatcgagc
     1981 agcttggcgc ccggaatgag ggccgcaagc tcggcccctg agccgccgat cacgtcttcc
     2041 gtaccgaccg cgacgaggac cgggaccctt atgccggcaa gaagttccgg cgtgatcttt
     2101 tcgcgcggac cgcgcatgca ggcggcaagt gctgcaaggt cgcccccggt atgttcggcg
     2161 aacgccctga agctccgtgc cgtatcattg gtcacctcgt cgatgctctt cgcctcgagc
     2221 gccttggcaa gcggccctgt ccccaccatg ccccgcacca tgttgatgcc gagcccggca
     2281 aggatggcgc tccgcacacg cgccggatgg ttgagcacca ggaacgccga gatgcgggct
     2341 cccatggagt agcccatgac gtcggcccgc tcgatgccga gatgatcgag caggcggcgg
     2401 gcatcttcgg ccatgatcgg cgcgccgtag cgctcgggtt catacagctt ctcgctttgc
     2461 ccatgccccc gattgtcgaa ggcaatgacg cggcggccgc tttcggtcag cgtgctgacc
     2521 caaccggggt cgatccaatt ggtcgcaaca ttggaggcaa agccgtggat gagcaggatc
     2581 ggctccccct ccccctcatc ccaataggca atgcgcacgc cgtcagattg gaagctcggc
     2641 attcctctat cgctcctgac cttcgccgac tatgctcacg cgcgacccac tctggaaaca
     2701 tatgacgctg ctgccatggc tcaaacgctg gtgcctcatt tcataaatga caagggcgtc
     2761 gaccgcatcg cgatcggcgt caaggaattc caatgcatgg gggcgacccc gcctttcgac
     2821 catccccatg tctatctcga tatgggggac gaggcgcagc tcgtctgccc ttattgctcg
     2881 acgctgttcc actacgatcc agccctcaaa gccacggaga gcgacccgcc gagttgtgtc
     2941 ttcgtggcga ccgccccgcc cggcgcaccg gaggcttgag cgctccggca atgacgaagt
     3001 ccgggccgca caaccgcgcg ctcatcgtcg gcggcggcat tggcggactt accacggccg
     3061 ttgcgcttgc gcgaaccggc atgagcgcca ccgttctcga gcgctcagct tttgccgacg
     3121 agaccggggc cggcatccaa cttgggccaa atgcgacccg cgccttggct caactcggcg
     3181 tgcttgatgc aattgccgca acggccttca gacccgatgt cctcaggctg ttcgacggcc
     3241 tgtcaggcac aagcctcgcc accgtgcctc tcggacagat cgccaaggag cgctacggcg
     3301 ccccctacct cacgcttcac cgcgccgacc tccacgcaag cctgctcgcc gcctgccgag
     3361 ccgaatgtgc catcgagctc aggagcggct tcgacgcaac cgaggccgag acactcgccg
     3421 agcctgtgat cgtccgtggc gatgatggaa cgcaggtcga gggctcaatt ctgattgccg
     3481 ccgacggtct ctggtcgcgc ttacgcgatc gcatcgctcc cgacgcagat ttgcgctttt
     3541 ccggcgccac cgcctggcgt gccttgttgc cgcgcggcga ggtgccggca ccgttcgccg
     3601 ccccggaggt cggactctgg ctcgggcccc gcgcccatct cgtccactac ccagtgcgcg
     3661 gcggcaagga tttgaacatc gtcgccgtgg tcgagggcgg cagcgccaag caaggctgga
     3721 gccgacgcgc cgaacccgat ctcctactgc caagcttcca gcgctgggct caacccgcca
     3781 agacgttgtt ggaggcggtc gagacttggc gctgctggtc gctctttcgg ctgaagccgt
     3841 tgccccgctg gacggacggg cgcatggcgc tcctcggcga tgccgcgcac cccgtgctgc
     3901 cctatctcgc ccaaggcgct gctctcgcca tcgaggacgc gatcacgctc gcggcaagcc
     3961 ttaaggccag cggtggcgat cctgcggccg cctttcctcg ctaccagtcg ctgcgcatgg
     4021 atcgcgctgc ccgcatccaa gcccaggccg cgcgtttcgg ccgcatgtac catctccgcg
     4081 gtccggcggc gttcgcacgc aattttctgc ttgggcgaag gcatcctgag tctctgctgc
     4141 aaagcttcga ctggctctac ggataggcga gccgctaagt ggcgggggtc gatttggccg
     4201 gggtcacggt aagcaggaca agagcgccca cgagcgccga cagcaaagag cccaccagca
     4261 ccgaaagctt gagcacggct tccgtttccg gatcagtaaa agccagcaag cctatgaaaa
     4321 gactcatggt gaagccgatg ccgcagagga ccgcgacgcc gtagatctgg cgccaactgg
     4381 ccccggcggg aagcaccgcg atgcgcgctt tcttggcgag gtaacccgca gaaagaattc
     4441 cgatctgctt gccgaggaag agaccgccga cgatgccgag cccgagcggc gacagaaccg
     4501 acatattcca cttgatggcc acgccggcat tggcgaaggc gaagatgggc agcaccagaa
     4561 acgccaccca gggagacagc gcgcgctcca tgctgtgcag cagcgagtgc cggccgtcgc
     4621 cagcgctatc gatggggatc aacatggcca gcaccacgcc ggcaagcgtt gcgtggatgc
     4681 ctgagaacag catcgccgtc cacagcagaa cgccaagcag gatatagggg agaagccgtg
     4741 tctccccgcg ccggttgagc caatagagcg cggccgcgaa aactgccgca acggcaagcg
     4801 ccgcgatgga gagattggaa gcgtagaaga ttgcgatgat gaggatcgcc ccgagatcgt
     4861 cgatgatggc gagcgaggtg aggaagacct tgagcgagcc cggcacccgc gagccgacca
     4921 gggcgagcac gccaagcgca aaggcgatgt cggtggcggt gggaactgcc cagccgcgcc
     4981 aattttccgg atg
//
LOCUS       DATNAE010000120         2625 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_1629839, whole genome shotgun sequence.
ACCESSION   DATNAE010000120 DATNAE010000000
VERSION     DATNAE010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1629839"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2002
                     /locus_tag="VN518_02580"
     CDS             <1..2002
                     /locus_tag="VN518_02580"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158526.1"
                     /note="Catalyzes the rate-limiting step in dNTP synthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HXE03489.1"
                     /translation="ATSAEMAAELGAFPRYAENRDVMLRVMRNHRRAAYGARTGYEEM
                     STPPVPLDHKAIADKALSDHAKLAWDRALTLGEEHGYRNAQASVVAPTGTIGLVMDCD
                     TTGIEPDFALVKFKKLAGGGYFKIINRAVPEALRVLGYGDSEIAEIITYAVGHGSLAD
                     APAINHGTLREKGFTDEAIEKIEKALPTAFDIKFVFNKWTLGADFLTEQLGVGKADLV
                     VPTFDLLAHLGFTRREIEAANIHVCGAMTVEGAPHLKPEHYAVFDCANPCGRTGKRYL
                     SVESHIRMLAAAQPFISGAISKTINMPNEATVEDCKQAYLLSWRLGLKANALYRDGSK
                     LSQPLQSQLIADETEDDDVIEAFLDKPAAARASGLAEKVVEKVVERIVVMRERERMPD
                     RRKGYTQKAVVGGHKVYLRTGEYDDGRLGEIFIDMHKEGAALRSLLNNFAIAVSLGLQ
                     YGVPLDEYVDAFTFTRFEPSGPVQGNDTIKYATSILDYVFRELAVSYMERFDLAHVDP
                     SGEGFDALGKGVEEGRPGPDVRYVSKGLTRSRTDRLKVMQGGVERSDAASPSPAAGGS
                     ASEASRSAVVTAFAATAPRAEIAGATVLKTTVEQKLSPAQALEQMTDPRAHAKAMAAE
                     RRAEAKAKGYEGEACGTCGNFTLVRNGTCLKCDTCGSTTGCS"
BASE COUNT          525 a          891 c          773 g          436 t
ORIGIN      
        1 tgccacgtcg gccgaaatgg cggccgagct cggcgccttc ccgcgctacg ccgaaaaccg
       61 cgacgtgatg ctgcgcgtca tgcgtaatca tcgccgcgcc gcttacggcg cgcgcaccgg
      121 ctacgaagag atgagcacgc cgccggtgcc gctcgaccac aaggccatcg ccgacaaggc
      181 cctctccgac cacgccaagc tcgcctggga tcgcgcgctc actctcggcg aagagcacgg
      241 ctaccgcaat gcgcaggcga gcgtcgtggc gccgaccggc accatcggcc tcgtcatgga
      301 ttgcgacacc accggcatcg agcccgactt cgcgctggtg aagttcaaaa aactcgccgg
      361 cggcggctac ttcaagatca tcaaccgcgc cgtccccgaa gctttacgcg tgttgggcta
      421 tggcgacagc gagatcgccg agatcatcac ctacgcggtg ggtcacggct cgctcgccga
      481 cgcgccggcc atcaatcacg gcacgctgcg cgaaaaaggt tttaccgacg aggcgatcga
      541 gaagatcgag aaggcgctgc cgaccgcctt cgacatcaag ttcgtcttca acaagtggac
      601 gctcggcgcc gacttcctca ccgagcaact cggcgtcggc aaggccgatc tggtcgtgcc
      661 gaccttcgac ctcctcgccc atctcggctt cacccgccgc gagatcgagg cagctaacat
      721 tcacgtctgc ggcgccatga cggtcgaggg cgcgccgcat ctgaaacccg agcactacgc
      781 cgtgttcgac tgcgccaatc cgtgcggccg caccggcaag cgttacctct cggtcgagag
      841 ccatatccgc atgctggcgg cggcgcagcc gttcatctcc ggcgccatct ccaagaccat
      901 caacatgccg aacgaggcga cggtcgagga ctgcaagcag gcgtatctgc tgtcctggcg
      961 gctcgggctc aaagccaacg cgctctaccg cgacggctcg aagctgtcgc agccgctgca
     1021 gagccagctc atcgccgacg agaccgagga cgacgacgtc atcgaagcct tcctcgacaa
     1081 gccggcggcg gcgcgcgcct ccggcctcgc cgagaaagtg gtcgaaaagg tcgtcgagcg
     1141 catcgtcgtc atgcgcgagc gcgagcgcat gcccgaccgc cgcaagggct acacccagaa
     1201 ggccgtggtc ggcggccaca aggtctatct gcgcaccggc gaatacgacg acggccggct
     1261 cggcgaaatc ttcatcgaca tgcacaagga gggcgcggcg ctccgctcgc tcctcaacaa
     1321 cttcgccatc gcggtgtcgc tcggcctgca atacggcgtg ccgctcgacg aatacgtcga
     1381 cgccttcacc ttcacccgct tcgagccgtc cggcccggtg cagggcaacg acaccatcaa
     1441 atacgccacc tcgatcctgg actacgtgtt ccgcgagctg gcggtgagct acatggagcg
     1501 cttcgacctc gcccacgtcg atccgtccgg cgaaggcttt gacgcgctcg gcaaaggcgt
     1561 cgaggaaggc agacccggcc ccgacgtgcg ctacgtctcc aagggcctca cccgctcgcg
     1621 caccgatcgc ctcaaggtca tgcagggcgg cgtggaacgc agcgacgccg cctcaccctc
     1681 ccctgcagcg ggaggttcgg cgagcgaagc aagccggagc gcggtcgtca ccgccttcgc
     1741 cgccaccgcc ccgcgcgccg aaatcgccgg cgccacggtc ctgaaaacca ccgtcgagca
     1801 aaagctctcg cccgcccagg cgctcgagca gatgaccgac ccccgcgccc acgccaaagc
     1861 catggccgcc gaacgccgcg ccgaagctaa agccaaaggc tacgaaggcg aagcctgcgg
     1921 aacgtgcgga aatttcacac tggtgcggaa cggcacgtgc ctgaagtgcg atacgtgcgg
     1981 aagcacgacg gggtgttctt aggctcaccc aggccgaaaa tgaaaaaggg cggcctcacg
     2041 ccgccctttt tcacagcttg tcgtcggatg aacagttatt tcccgctctt atgatgagcg
     2101 ggcttcacaa gcctccaacc tgaattgcca ggctcggttg gcggtaacgg cttcccccta
     2161 gtggagtcgg cttgttcacc ggtctttccg ccgcgcgggc caacctgctc gtaaattccc
     2221 gaacgcggcg ttggagtgcc tggcggcatt gggtttttcg acatggaagt tctcctgcgg
     2281 tcaacccaga gagccgatca gccctcctgt tcacgatatg taccagtcct tgacttcaaa
     2341 gtctagcgtt cgacccggga tgcagcacaa aatgttgtgg gaatgcggca atatccgtgc
     2401 gaatcttcga cttggaactt agactcaaag atgattcggg gggcttgcgt ggattccttt
     2461 ctgcagatgg cagcacaaaa gcggccgatt gccattgacg atattgaggc cattgagagc
     2521 ctcgcgcgaa tacttcgcaa agctctggaa ataaacgatc gtactgctcc gaacatgctt
     2581 tacgtgttcg agcaactgaa aaagattttt cctaaactca agctg
//
LOCUS       DATNAE010000121         2781 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3430221, whole genome shotgun sequence.
ACCESSION   DATNAE010000121 DATNAE010000000
VERSION     DATNAE010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3430221"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="VN518_02585"
     CDS             <1..674
                     /locus_tag="VN518_02585"
                     /inference="COORDINATES: protein motif:HMM:NF028211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HXE03490.1"
                     /translation="VGTTDVAHAGDPGEARCSEEEKAYLLAAYNRYFAGPRPATPTDV
                     VFTWSGVRALHDDAGARPSRISRSPELSSVANGTGGFVSLYGGKLTTHRAMAEEVLET
                     LQKLGAAMSGAWTKDVALYGGTLTKPELLACADRGPRGIADATRRRLALTYGDKIETL
                     FARIATTPALAEEIAPGVTRAELDHARETEDAMTAEDFLLRRTKLYLTLSPSGRDAIE
                     RWFLG"
     gene            complement(671..1126)
                     /locus_tag="VN518_02590"
     CDS             complement(671..1126)
                     /locus_tag="VN518_02590"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HXE03491.1"
                     /translation="MQGILALGAWNWMILAAILFVLEVVSPGIFLMWFGVAAVATALI
                     VFRYDLAWQWQLIWFCGLSLVSVLLAAKYLRKHPLDSERPLLNERAVQLIGQSFVLID
                     PIVDGKGSIKTGDTIWRVEGPELPKGARIKVVGADGTVLKVVPGAAAAA"
     gene            complement(1144..2145)
                     /locus_tag="VN518_02595"
     CDS             complement(1144..2145)
                     /locus_tag="VN518_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012652336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HXE03492.1"
                     /translation="MFGFNIFVIALLILFIAVIAAGVKIVPQGWNYTVERFGRYTRTL
                     RPGLTLIIPFIDRIGRKLNVMEQVIEVPSQEVITKDNAMVRVDGVAFFQVLDAAQASY
                     EVTNLQIAILNLTMTNIRTVMGSMDLDKLLSERDEINHRLLKVVDDATHNWGVKVTRI
                     EIKDIEPPRDLIDSMARQMKAERDKRAAILVAEGERQAAILTAEGSKQAQILAAEGRR
                     EAAYRDAEARERSAEAEAKATQVVSEAIGKGSVQAINYFVANNYIGALKALAEAKNQK
                     VLILPLEATSVIGAIAGVSEIAREAFGSGGDSASPQPGQTKASAGQRPRAGGSVPSV"
     gene            complement(2287..2730)
                     /locus_tag="VN518_02600"
     CDS             complement(2287..2730)
                     /locus_tag="VN518_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03493.1"
                     /translation="MMRAAVIAALSLVVGIWAGAPAAAETALPAEAAGPLAYVTVAGA
                     PGDGEEALAAALSRQLAARGLAPATAFQANVYEVQGTVRVAPGAKGKQSITIIWVVLA
                     PDGTQLGVTRQTKDVRKGSLDKKWGPAANAAAAAAAEDITKLIPK"
BASE COUNT          463 a          939 c          884 g          495 t
ORIGIN      
        1 tggtcggcac gaccgacgtg gcccatgcgg gcgatccggg cgaggcgcgc tgctccgagg
       61 aggagaaagc ctatctgctg gctgcctata accgctattt cgccggcccg cgccccgcga
      121 cgcccacaga tgtcgtcttc acctggtccg gcgtccgcgc gctgcacgat gatgcgggtg
      181 cacggccgag ccgcatcagc cggagccccg agctgtcctc ggtcgccaac gggactggag
      241 gcttcgtcag cctctatggc ggcaagctca ccacgcatcg cgccatggcc gaagaggtgc
      301 ttgagacgtt gcagaaactc ggggcggcca tgagcggcgc ctggaccaag gacgtagcgc
      361 tttacggcgg cacgctcact aagcccgagc ttctcgcctg tgccgatcgc ggtccgcgcg
      421 gcatcgcgga cgcaacgcgc cgccgtctgg cactgacgta tggcgacaag atcgagacgc
      481 tgtttgcgcg catcgccacc actccggccc tggccgagga gatcgctccc ggcgtgaccc
      541 gcgccgagct cgaccatgcg cgagagaccg aggacgccat gacggccgag gacttcctgc
      601 tgcggcgcac caagctctat ctcacactca gcccgagcgg gcgagacgcc atagagcgct
      661 ggttcttggg ctaggccgcg gctgccgctc ccggcaccac cttcagcacc gtgccgtcgg
      721 cgccgaccac cttgatgcgc gcgcctttgg gcagctcagg gccttccacc cgccagatcg
      781 tgtcgccggt cttgatgctg cctttgccat cgacgatcgg atcgatcaaa acgaaggatt
      841 gcccgatcag ctgcaccgcg cgctcgttca agagcggccg ctcgctgtcg agtggatgct
      901 tgcgcagata tttggcggca agcagcaccg aaaccagcga aaggccgcag aaccaaatga
      961 gctgccactg ccaggcgagg tcgtagcgaa acacgataag agccgtggcg accgcggcca
     1021 cgccgaacca catcagaaag ataccgggag acaccacctc caggacgaac aggatggcgg
     1081 cgaggatcat ccagttccag gcgccgagtg cgagaatgcc ttgcatggat cgcttcccgc
     1141 ccactagacg gatgggacgg agccgcctgc gcgcgggcgc tgaccagctg acgccttggt
     1201 ctgacccggc tgcggcgacg ccgaatcgcc gccgctcccg aaggcttcgc gggcgatctc
     1261 ggagacgccg gcgatggcgc caatcaccga cgtcgcttcg agtggcagaa tgagcacttt
     1321 ctggttcttc gcctcggcga gcgccttcag cgcaccgata tagttgttgg cgacgaaata
     1381 gttgatcgcc tgcacgctgc ctttgccgat cgcctcgctg accacttgcg tggccttggc
     1441 ctcggcctcc gccgaacgct cgcgtgcctc ggcatcgcga taagccgcct cgcggcggcc
     1501 ctcggcggcg agaatttgtg cttgcttgga gccctcggcg gtcaggatcg ccgcctgccg
     1561 ctcgccctcg gccaccagga tcgccgcgcg cttgtcgcgc tcggccttca tctgccgcgc
     1621 catcgaatcg atcagatcgc gcggcggctc gatgtccttg atctcgatgc gggtgacctt
     1681 gacgccccaa ttatgcgtcg cgtcgtccac caccttgagc aggcggtgat tgatctcgtc
     1741 gcgctcggat agcagcttgt cgaggtccat ggagcccatc accgtgcgga tattcgtcat
     1801 ggtgagattg aggatggcga tctgcagatt ggtcacctcg tagctcgcct gtgcggcatc
     1861 cagcacctgg aagaaggcca cgccatccac cctcaccatg gcgttgtcct tggtgatgac
     1921 ctcctggctc ggcacctcga tcacctgctc catgacattg agcttgcgcc cgatgcggtc
     1981 gatgaaggga atgatcaggg tgagcccggg cctgagcgtg cgcgtatagc gcccgaagcg
     2041 ctccaccgtg tagttccagc cctgcggcac gatcttcacg ccggcggcaa tcacggcgat
     2101 gaacagaatc agcagagcta tgacgaagat attgaagccg aacatggcgc cctcccggag
     2161 gcgtttggct agaggttctt ggttaagtct tggcggtctt ctggctagcc ttcctcgcgg
     2221 cagataagtg ggtgcgaggt cgatgaaata taagatgctc gacgcaggct ccccgtttaa
     2281 tcgagcctat ttggggatga gcttggtgat gtcctcagcc gccgccgcgg ccgcggcatt
     2341 ggcggccggc ccccattttt tgtcgagcga gcctttgcgc acatccttgg tttggcgggt
     2401 gacgccgagt tgcgtgccgt cgggcgcgag caccacccag atgatggtga tgctctgttt
     2461 gcctttggcg ccaggcgcga cccgcaccgt gccctgcacc tcgtagacat tggcctggaa
     2521 ggcggtcgcc ggggccagcc cgcgcgcggc aagctgccgg ctgagcgcgg ccgcgagcgc
     2581 ctcctccccg tcgcccggcg cgccggcaac agtcacatag gccaggggtc ccgcggcttc
     2641 cgccgggaga gcggtctccg cagcggcggg tgcccccgcc caaattccga ccaccaggct
     2701 cagcgccgcg atgacagccg ctcgcatcat tcaacacgtc ttcttggcgc gctcgagctc
     2761 gaccagcttc cggtctttga c
//
LOCUS       DATNAE010000122         5062 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_4334820, whole genome shotgun sequence.
ACCESSION   DATNAE010000122 DATNAE010000000
VERSION     DATNAE010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5062
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4334820"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..155)
                     /gene="yacG"
                     /locus_tag="VN518_02605"
     CDS             complement(<1..155)
                     /gene="yacG"
                     /locus_tag="VN518_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012454628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HXE03494.1"
                     /translation="MKPKRAKSAKGPRCPICGKPSVQDYRPFCSRACADEDLGRWLGG
                     RYVVPGER"
     gene            complement(179..898)
                     /locus_tag="VN518_02610"
     CDS             complement(179..898)
                     /locus_tag="VN518_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013417975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HXE03495.1"
                     /translation="MGGSPEPAESGAPAKSRLTPSPRASKSSGHRPKLVLASASPRRL
                     ALLEQVGIIPDALRPATIDETPTRGEVPRHLVQRLARAKAEAAKLLVRAEPDYAKSFI
                     LAADTAVAVGRRILGKPDEGNRATETLERLRGRNHRVYSAICLITPDDRKRERVVETR
                     IRFRNFSDAEIRDYIASGEWKGKAGGYAIQGIAGGFVVKLVGSYTSVVGLPLTEVVTL
                     LQGEGYPVSYFWPNLAEIETE"
     gene            complement(903..1121)
                     /gene="infA"
                     /locus_tag="VN518_02615"
     CDS             complement(903..1121)
                     /gene="infA"
                     /locus_tag="VN518_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HXE03496.1"
                     /translation="MAKEEMLEFPGVVTELLPNATFRVKLENDHEIIAHTAGRMRKNR
                     IRVLAGDKVLVEMTPYDLTKGRITYRFK"
     gene            1332..2084
                     /locus_tag="VN518_02620"
     CDS             1332..2084
                     /locus_tag="VN518_02620"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HXE03497.1"
                     /translation="MRFSRSILAMAAVAALGFSGLVLPLAAQAQDVKSADAIVKGLAP
                     VKTRGFDPLAPEREAKQQELNAKLREFKTRQIEVIPREDRDQVAKLVEESKSPNVDVQ
                     ILFAFDSAEVLPEARPALDELGKALSDPKLSGGTFLIAGHTDAKGSDAYNLGLSQRRA
                     ASVKAFLVETYHVDGGRLSVIGFGEEQLKNKEDPLADENRRVQIVNTGNATVAEGKPA
                     PADAPAPEAAPPAEAAPPADAAPPAEAAPPPQ"
     gene            complement(2087..2560)
                     /locus_tag="VN518_02625"
     CDS             complement(2087..2560)
                     /locus_tag="VN518_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphatase family protein"
                     /protein_id="HXE03498.1"
                     /translation="MGRADALGPGEADLPGAVLFACSRNSVRSPMAAAILKHLAGRRI
                     YVDSAGVRAGEPDPFAIAVMEELGIDLSGHRPAALADLHDTSFDLIVTLSPEAHHKAL
                     ALAGSFAIDVEYWPTPDPTMTSGNREQILSAYRAVRDGLFRRIKQRFPFAGAPGV"
     gene            complement(2547..3053)
                     /locus_tag="VN518_02630"
     CDS             complement(2547..3053)
                     /locus_tag="VN518_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0262 family protein"
                     /protein_id="HXE03499.1"
                     /translation="MTEGQQQQQELSPEQARLVEVTLDSGSIGRNSVDVEHERAVAIF
                     DLLERNSFALENHDGGPYTLHLSLADNRLVFTVGDEKRNPIVHVMLSLSPFRRLVKDY
                     FLMCESYYQAIKTQPPSRIEAIDMGRRGLHDEGSRLLMERLKGKVLVDIATARRLFTL
                     ICALHWKG"
     gene            complement(3060..4358)
                     /gene="hisD"
                     /locus_tag="VN518_02635"
     CDS             complement(3060..4358)
                     /gene="hisD"
                     /locus_tag="VN518_02635"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HXE03500.1"
                     /translation="MALWLEQRAADFETRFGALLAAKRESSADVNEAVAAIIDRVRRD
                     KDAGLIDLTLRYDRVDLRKIGMCVTPAEIAEAKATVDAKTLAALELARDRILDHHRRQ
                     VPASDCYVDDQGVALGFRWTPVQSVGLYVPGGTASYPSSVLMNAVPAKVAEVERVVMV
                     VPAPEGKLNPLVLVAAALGGVDEIYRIGGAQAIAALAYGTETIAPVVKIVGPGNAYVA
                     AAKRQVFGQVGIDMIAGPSEVVVIADKDNDPAWIAADLLAQAEHDAAAQSILITDDAG
                     FGRAVVAAVKAQLATLPRASVAGASWENFGAVIVVESLDEAPVLVDRLAPEHLEIATE
                     DPESLSAAIRNAGAIFLGRYTPEVIGDYVAGPNHVLPTARSARFASGLGVLDFMKRTS
                     ILKLEADSLSALGPAAMTLARAEGLEAHRRSVAIRLNLRD"
     gene            complement(4380..4844)
                     /locus_tag="VN518_02640"
     CDS             complement(4380..4844)
                     /locus_tag="VN518_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020088022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2948 family protein"
                     /protein_id="HXE03501.1"
                     /translation="MADNQLKLIALDEQDLEIVSSHLQDAVVRVADLAYVPSQKRFAA
                     ILNRFNWEKAAKAAPSDTNERRRTALRFDRVLGAQIKSLKTSASDRVLSLLAVRFEPS
                     EHPSGFVTLTFSGDASIRLHVECIEAELTDLGPAWRARSKPQHPGAEPGADS"
BASE COUNT          862 a         1623 c         1709 g          868 t
ORIGIN      
        1 cgctcgccag gcacgacgta gcggcctccg agccagcggc cgaggtcttc gtcggcgcag
       61 gccctgctac agaagggacg gtagtcttgg acggagggct tgccgcagat cgggcatctc
      121 gggcccttcg cgcttttcgc ccgcttgggc ttcacccctt cggctcgggc cttcggcgtc
      181 actcggtctc gatctcggcg aggttcggcc agaaatagct caccggatag ccctcgcctt
      241 gcagaagcgt caccacctcg gtgaggggga gccccacgac actcgtgtag gagccgacga
      301 gcttcaccac gaaccctccg gcgatgccct ggatggcgta gcctccagcc ttccccttcc
      361 actcgccgct cgcgatatag tcgcggatct cggcgtcgga gaaattgcgg aagcggatgc
      421 gggtttcgac cacgcgctcg cgcttgcggt cgtcgggcgt gatcaggcag atggcgctat
      481 agacgcggtg gttgcggccg cgcaagcgct ccagcgtctc ggtcgcccgg ttgccctcat
      541 cgggcttgcc gagaatgcgc cggcccacgg cgaccgcggt gtcggcggca agaatgaagg
      601 acttggcata gtcgggctcg gcgcgcacca ggagcttggc ggcctcagcc ttggcccggg
      661 caagccgctg caccaaatgg cgcggcacct ctccgcgcgt cggcgtctcg tcgatggtgg
      721 ctggtcggag cgcatcagga atgatgccaa cctgctcgag cagagcgagc ctgcgcggcg
      781 aggccgaggc aagcacgagc ttcggcctgt gcccgctcga cttcgaggcg cgcgggctcg
      841 gcgtcagcct gctcttcgct ggcgcaccgc tttcggcagg ctctgggctt ccgcccacga
      901 gcctatttga agcggtacgt aatccgtccc ttagtgagat catagggcgt catctcgacc
      961 agaaccttgt cgccggcaag caccctgatc cggttcttgc gcatccgtcc cgccgtatgg
     1021 gcgatgatct cgtgatcgtt ctcgagtttc accctgaagg tggcgttggg gagcagctcg
     1081 gtcactaccc ccggaaattc cagcatttct tccttcgcca taaggcctct ttcggcgttc
     1141 cgcagattgg ggcggagaat ggtcggggga cgcggcaaaa tcaaggagga ttactggtgc
     1201 tggctctcgc cggttgccgc tttcggcttt gccgccgcca tcccccgttc atggcaggtt
     1261 cagcttgccg tatttagggt ctaagcttgt cattgggctt ggctagactg cgatcgcgga
     1321 ggttacgagc catgcggttc tcaaggtcga ttttggcgat ggcggcagtg gccgcgttgg
     1381 gcttctcagg gcttgtgctt ccgctggccg cacaggcgca ggacgtgaaa agcgccgatg
     1441 ccatcgtcaa gggattggcc ccggtcaaga cccgcggctt cgatccgctc gcgcccgagc
     1501 gcgaggccaa gcagcaggag ctgaacgcca agctgcgcga attcaagacg cggcagatcg
     1561 aggtgatccc gcgcgaggac cgtgaccaag ttgcgaagct cgtcgaggag tcgaagtcgc
     1621 cgaacgtcga tgtgcagatc ctttttgcct tcgactcggc cgaggttctc cccgaggcgc
     1681 ggcccgcgct cgacgagctc ggcaaggcct tgagcgatcc caagctcagc ggaggcacgt
     1741 tcctcatcgc ggggcatacc gacgccaagg gcagcgatgc ttacaacctc ggtttgtcgc
     1801 agcggcgcgc agcgtcggtg aaggcattcc ttgtcgagac ttaccatgtc gacggcggac
     1861 gcctctcggt gatcgggttc ggcgaggagc agctcaagaa caaagaagac ccgctcgccg
     1921 acgagaaccg gcgggtgcag atcgttaaca cgggaaatgc cactgtggcg gaaggcaagc
     1981 ccgcgccggc agacgccccg gccccggaag cagcacctcc tgccgaggcg gcccctccag
     2041 ctgacgcggc gcccccggcc gaggcagccc ctccccctca ataagctcac acgccaggtg
     2101 ctccggcgaa ggggaagcgc tgcttgatgc ggcggaacaa cccgtcgcgc accgcgcggt
     2161 aggccgacaa gatctgctcg cggttgccgc tcgtcatggt ggggtcaggc gtcggccaat
     2221 attccacgtc gatggcaaag ctgccggcaa gggcgagcgc cttgtgatgc gcctccggcg
     2281 acagcgtcac gatcaggtcg aaggaggtat cgtgcaggtc ggcaagcgcg gccggccgat
     2341 ggcccgaaag gtcgatgccg agctcttcca tgacggcgat ggcgaagggg tccggctcgc
     2401 cggcgcgcac gccggccgag tcgacataga tgcgccgtcc ggcgagatgt ttgaggatcg
     2461 cagccgccat tggcgagcgc acggagttgc ggctgcaggc gaagaggacc gcgcccggca
     2521 ggtcggcttc tccgggcccc aaggcatcag cccttccaat gcagggcgca gatcagggtg
     2581 aacaggcgtc tcgcggtggc gatgtcgacg agtaccttgc ccttgagccg ctccatgagg
     2641 agccggctgc cttcatcgtg caagccacgc cgtcccatgt cgatcgcctc gatgcgcgac
     2701 ggcggctggg tcttgatcgc ctgatagtag ctctcgcaca tcaggaagta gtccttcacc
     2761 aggcggcgaa agggcgacag cgagagcatc acatgcacga tggggttgcg tttctcgtcg
     2821 ccgacggtga acaccaggcg attgtcggcg agcgacagat gcagcgtgta agggccgccg
     2881 tcatgattct cgagcgcaaa ggagttgcgc tcgagcagat cgaagatggc gaccgcgcgc
     2941 tcatgctcga cgtccaccga gttgcggccg atcgatccgg aatcgagcgt cacctcgacg
     3001 agccgcgcct gctcgggtga aagctcctgc tgctgctgtt gcccctcggt catttttgat
     3061 cagtcccgca ggttcaaccg aatggcgacg gatcggcggt gggcttcgag cccttcggcg
     3121 cgcgccaagg tcatggctgc aggcccgagc gcgctcaagc tatcggcttc aagcttgagg
     3181 atcgaggtgc gcttcatgaa gtcgagcacg ccgaggcccg aggcgaagcg ggcgctccgc
     3241 gccgtgggaa ggacgtggtt cggccccgcc acatagtcgc cgatcacctc gggcgtgtaa
     3301 cggccgagaa agatcgcgcc ggcgttgcgg atcgctgccg acaaggactc gggatcctcg
     3361 gtggcaattt cgagatgctc cggtgcgaga cgatcgacaa gcactggcgc ttcgtcgagg
     3421 ctctcgacca cgatgacggc gccgaaattc tcccagctcg ccccggccac gctcgcgcgc
     3481 gggagcgtcg caagctgggc tttgacggcg gcgacgacgg cgcggccgaa gcccgcgtcg
     3541 tcggtgatca ggatcgactg cgctgctgcg tcgtgctcgg cctgggcgag gagatcggcg
     3601 gcgatccagg cgggatcgtt gtccttatcg gcgatgacca cgacctctga cgggcccgcg
     3661 atcatgtcga tgccgacctg gccgaacacc tggcgcttgg cggccgccac ataggcgttg
     3721 ccgggtccta cgatcttgac cacaggcgcg atcgtctcgg tgccataggc aagggccgcg
     3781 atcgcctgtg ccccgccgat gcgatagatc tcatcgacgc ccccaagcgc cgccgccacg
     3841 agcaccagag gattgagctt gccctcgggc gccggcacca ccatgacgac gcgctcgacc
     3901 tctgccacct tggcgggaac cgcattcatc agcaccgagc ttggatagct cgctgttccc
     3961 cccggcacat aaagcccgac ggactgaacc ggcgtccagc ggaagccgag cgccacgccc
     4021 tgatcgtcga catagcagtc gctcgccggc acttggcggc ggtgatggtc gaggatgcgg
     4081 tcgcgggcaa gctcgagcgc ggcaagggtc ttggcgtcga cggtggcctt cgcctcggcg
     4141 atctcagcgg gggtcacgca catgccgatc ttgcgcagat ccacgcggtc gtaacgcaag
     4201 gtcagatcga tcaggccagc gtccttgtcg cggcgcacgc ggtcgatgat cgcggcgacc
     4261 gcctcattga cgtcggcgga ggattcccgc ttggccgcaa gaagcgcgcc gaagcgggtc
     4321 tcgaagtccg ccgccctctg ctcgagccat aaggccatgg tgggggggag gcctgaggat
     4381 caggagtcag ccccgggctc tgcgccaggg tgctgcggtt ttgagcgggc gcgccaagca
     4441 ggcccaagat cggtgagctc ggcctcgatg cattcgacgt ggaggcggat cgaggcatcg
     4501 ccggaaaacg tcagcgtgac aaagccgctc gggtgctcgc tcggctcgaa gcggacggca
     4561 agcagcgaca gcacccggtc agaggcagaa gtcttcagcg acttgatctg ggcgccgagc
     4621 acgcgatcga agcgcagcgc cgtacgcctg cgttcgttgg tgtcgctggg ggcggccttc
     4681 gccgccttct cccagttgaa gcggttgaga atggcggcga agcgcttttg cgatggcacg
     4741 taggccaaat ccgcgacgcg caccacagca tcctgcaggt gcgacgacac gatctcgaga
     4801 tcctgttcat cgagcgcaat cagcttaagt tggttgtcgg ccatcggggc atcccggctg
     4861 ggaccgtaac cgcctgccga taggcggaaa tgcggtctct tcgtcattcg tcggcgctag
     4921 ggcctagcat gctagccgac gaggcgaaac cggcaaagtg ccgaaagcgg gcctagccag
     4981 aaagccgttc gatttgggcg ccgcacttcc gcagcttgtc ctcgatgcgc tcgaagccgc
     5041 ggtcgagatg gtagacacgg cc
//
LOCUS       DATNAE010000123         7128 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_420957, whole genome shotgun sequence.
ACCESSION   DATNAE010000123 DATNAE010000000
VERSION     DATNAE010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 7128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7128
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420957"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..855
                     /locus_tag="VN518_02645"
     CDS             130..855
                     /locus_tag="VN518_02645"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HXE03502.1"
                     /translation="MRVLVTRPEPDATSQAERLAARGHEPVLAPLLAIEWASGVPLEL
                     DGAQALIVTSRNALRALASHRDLAASLQLPLFAVGEATAKAATELGFTTVIAGPGTGE
                     ELSRLIARTLKPNAGALVHLAGETVAFDLKSALQAKGFTLRQPVLYRAVAATRLPESV
                     LSLLNASKLDGVILMSPRTAAIFAALMVRHGALTQASRLDCYCLSAAVAQAVEPLRAR
                     AIVAVRPYEDDILALLDSDAASS"
     gene            895..2262
                     /locus_tag="VN518_02650"
     CDS             895..2262
                     /locus_tag="VN518_02650"
                     /inference="COORDINATES: protein motif:HMM:NF021264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mitofilin family membrane protein"
                     /protein_id="HXE03503.1"
                     /translation="MAEPFGDQLNPGSGKRPAPTIEGTATEVSIDAAPAETGSPDPAV
                     EAETSDPEGSEPKHGPKKPPPRTSPTELKGFFTHLAAGLLGGLIGVVALALFWNKLPV
                     RNAAAPDLTLVNSRLAKLESAPPPAGDTAALGALDARVKTLEDRKVETPPDLSDLTSR
                     VTRLEESINALAETAKEGGSVPDAAALDAKIGDLEQRLQGKIDSALAAQQGESTTELK
                     DLQKEVEALKAKLGALAEAHLAGDTSDLAPQLTTLDQRIAKLEATLPELSTAIDRSSA
                     SAKSGAAAIAFANLRNAVAAGRPYAAELAALKSLIPDSGDLGVLPSHAETGIPTVAAL
                     ASDLTKLAETSAAPPPAPAQTSILDSMMASAKSAISIRRIGADATGDEPAAVLARAEA
                     ALNQGDLAAAIKEVESLPAQERDPFAGWRADAQARASANDTLSKLESTVLVSLGGGAA
                     PEAKP"
     gene            2267..3919
                     /locus_tag="VN518_02655"
     CDS             2267..3919
                     /locus_tag="VN518_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HXE03504.1"
                     /translation="MIRVLVFVIGVIAAALSLAWLADRPGAITIEWLGYEIKTSAFVG
                     ALALAALVTVLILAWALLRYLFSRPAAIAAYRQERRRRQGFDALTRGLLAIGVGDRAQ
                     AQRYAGVARRNLPNEPLTALLKAQAAQLKGDRASARRAFEAMLDRPETELLGLRGLFL
                     EAKRGNDNGAARALAEQAVRTDPQAGWSVNALFDMQAREGDWEGALATLAVARKHGNV
                     EPDAALRRRAVLLTAEARDLEASQPDKALALATEALRLAPSLIPAAEIAGRLLASKGE
                     SRQASRLVARTWKLAPHPDLATVYAFAKPGESPRDRLRRVEHLASLTPGDIEGPIAIA
                     VAAIEARDWQRAEDAISPYLHDRPPARVCALMARIEAGEGNKGREREWLARAVRGPRD
                     RAWIADGYISDRWLPVSPVTGAVDAFEWKAPVDAIGRGDATLLIEESHEPPEPPPALK
                     VAAPVKETAVPKKPELVSPRPLVAGDGAAPLRPAESGQMRPAVPPVTPVPPAAPREQP
                     PQARPKPEFYVAPPIPDDPGVAPADPDESPASLERLRAAQIR"
     gene            4022..4097
                     /locus_tag="VN518_02660"
     tRNA            4022..4097
                     /locus_tag="VN518_02660"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4055..4057,aa:Thr,seq:cgt)
     gene            complement(4121..5425)
                     /locus_tag="VN518_02665"
     CDS             complement(4121..5425)
                     /locus_tag="VN518_02665"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017962122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HXE03505.1"
                     /translation="MEGLPVSLAERLQELTYDERAVVYLQVDASLTLIDAGGHLDNYG
                     LAALRLGEPAVEQAFFLEGLLPLVESPYFVPSIELDCGRAADLHLHQDGDTVWVVLFD
                     VTEQRDATRRLQQKAYDMTLLQEKEAELNRRLADMNRELEASNDFLARISSKISHYLA
                     PQIYKSIFSGQRDVTIHTERKELTIFFSDIKDFTSTTEHLQPEEITLLLNEYFTEMSA
                     IAHNHGGTVDKFIGDALVIFFGDPETKGTVEDARACLNMATEMQRRLAELNVKWRSAG
                     TEQPFRVRMGVNTGFCNVGNFGSMHRMDYTAIGAEVNLAARLQSIAEPGHIVISYDTY
                     VLVRDIVAARALPEITVKGIGRMVVPYVVEGVLDEAGRKIEIFSEHMTGLDFYLDPRA
                     IDATAIERIRATLKNAIAALEGRGGEDAPAPVARSDRMDPGL"
     gene            complement(5429..5932)
                     /locus_tag="VN518_02670"
     CDS             complement(5429..5932)
                     /locus_tag="VN518_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rab family GTPase"
                     /protein_id="HXE03506.1"
                     /translation="MTPVLQKKICMLGGFSVGKTSLVKRYVQSIFSETYLTTVGVKID
                     KKTVDLSDRIVNLILWDLAGEDDINTFRMTNMRGAAGYVLVADGTRPPTLDVALSLRQ
                     RVETEYGPLPFVLLLNKSDLKEEWAIGDAEVEGLRRNGLWVQPSSARTGEGVEDAFKD
                     LATRASS"
     gene            complement(5929..>7128)
                     /locus_tag="VN518_02675"
     CDS             complement(5929..>7128)
                     /locus_tag="VN518_02675"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HXE03507.1"
                     /translation="ARLRAEPGIVITRSEARDGKFLLSGLRDPLAVDPLKLLGEAGID
                     PARVEAHFEPYQGLDPQSVLKRLTATLDPPPSVTLAIAGNRIVARGSAPRPWLDRAHA
                     IAQMLPAGAPSFDLAGVRNDDEADEQRRQVDERQWQAYVARLRAEPGIVITRSEASDD
                     KFLLSGLRDPLAVNPLALLSEAGIDPARVEAHFDPYEALDPQFVLKRLQASLNPPPGV
                     TLAVDGDRIVAQGSAPSPWISRARKVGSSLPAGAPVLDLSAMRDISEGALGKLRDAIQ
                     ARSINFDSGKSLPAAGQEQVLDEIANDLNELVKLSSTLHAVTRMTLTGHADSQGQGTS
                     NLSLSMARAEAVRALLKKRGVDPDLLQVRGAGQLEPVADDTPAAAAAARSANRRVSFS
                     IGFEEEP"
BASE COUNT         1192 a         2449 c         2301 g         1186 t
ORIGIN      
        1 ccgaatggca tgagatcgag aagagcggcg cccctgaaga tgccgtgggc atcggcgcaa
       61 gctcaggccg cgagctgctc gcgcttgccg gtccgaattt cctcgccacc ctcacggctc
      121 gctgatgtga tgcgcgtcct cgtcacgaga ccggaaccag acgccaccag ccaggctgag
      181 cgcctcgccg cgcgcggtca cgagccggtg ctcgccccgt tgctcgccat cgaatgggca
      241 agcggcgtgc cgctcgagct cgatggcgca caggccctga tcgtgaccag ccgcaatgcc
      301 ttgcgcgcgc ttgcctctca ccgcgacctc gccgcctcgt tgcaacttcc gctcttcgct
      361 gtcggcgaag ccacggcgaa agccgcgacg gagctcggat tcaccacggt gattgccggc
      421 cccggaacgg gcgaagaatt gtcgcgcctg atcgcccgca cgctcaagcc aaatgccggg
      481 gcgctcgtgc atcttgccgg cgaaaccgtc gccttcgact tgaaatcggc gctgcaagcc
      541 aaaggcttca cgctgaggca gcccgtgctc tatcgggccg ttgcggcgac gaggctgccg
      601 gagagcgtgc tgtccttgct gaacgctagt aagctcgacg gcgttatcct gatgtcgccg
      661 aggacagccg ccattttcgc ggctttgatg gtgcggcacg gcgcgctaac gcaagcttcg
      721 cgacttgatt gctattgcct ttcggcggcg gtagcacagg ccgtggagcc gctaagggcc
      781 agggcaattg tggcggtgcg gccgtacgag gatgatatcc tcgcgctcct cgactccgac
      841 gcggcatcct cgtaaggatc gcgtcccacg atttgaacat cgacggtagg cggaatggct
      901 gagccttttg gcgatcaact gaacccgggt tccggcaagc ggccggcgcc gaccatcgag
      961 ggcaccgcga ccgaagtgtc catcgacgcg gcgccggccg agaccggttc gcccgatcct
     1021 gccgtagagg cggagacgtc cgatccggaa ggatcggagc cgaagcacgg tccgaagaag
     1081 cctccgccac gcacgagccc gaccgagctc aaggggttct tcactcatct cgccgccggg
     1141 ctgctcggcg gcctcatcgg tgtcgttgcc ctggcgcttt tctggaacaa gcttccggtg
     1201 cggaacgcgg cagcgcctga cctcactctg gtcaacagcc gcttggcgaa gctcgaatcg
     1261 gcacctcccc cggcagggga cactgcggcg cttggcgcgc tcgatgcccg cgtcaagacc
     1321 ctggaagacc gcaaggtcga aacgcctccc gacctctccg atctgacctc tcgcgtgacg
     1381 cgcctcgaag agtcgatcaa tgccttggcc gagacggcca aggaaggcgg ctcggtgccc
     1441 gacgcggccg cgctcgacgc caagatcggc gatctcgagc agaggctgca aggcaagatc
     1501 gacagcgcgc ttgccgccca acaaggcgaa agcacgaccg aactcaaaga tctgcaaaag
     1561 gaggtcgagg cactcaaggc aaagctcggc gcgctggccg aagcgcatct tgccggcgac
     1621 acgtcggacc tcgccccgca gctcaccacg ctcgatcagc gaattgccaa gctcgaagcg
     1681 accctccctg agctttccac cgccatcgac cgctcgtcgg caagcgccaa gtccggagcc
     1741 gcggctatcg cctttgccaa tttgcgtaac gcggtcgccg ccgggcgtcc ctacgccgct
     1801 gagcttgccg cgctcaaatc tctcattccc gattctggcg atctcggcgt gctcccctct
     1861 cacgccgaaa ccggcatccc gacggtcgcc gcgctcgcaa gcgacctgac aaagctcgcc
     1921 gagacgagtg ccgctcctcc gccggccccc gcccagacct cgatcctcga cagcatgatg
     1981 gcgagcgcaa agtcggcgat ttccatccgc cgcatcggcg ccgacgcgac cggggacgag
     2041 cctgccgccg tgctggcaag agccgaagcg gcgctgaacc aaggcgatct tgccgccgcc
     2101 atcaaggagg tcgagtcgct gcctgcgcaa gagcgcgatc cgtttgccgg ctggcgggcc
     2161 gacgctcagg cccgggcctc cgccaacgac acattgagca agctcgaaag cacagtgctc
     2221 gtctccctcg gcggtggcgc ggcccctgaa gcaaagcctt gaaccgatga tccgcgtcct
     2281 cgtcttcgtc attggcgtca tcgccgcggc cttgtcgctc gcttggctcg ccgaccggcc
     2341 tggcgccatc accatcgaat ggctcggcta cgagatcaaa accagcgcct tcgtcggggc
     2401 gcttgctctt gccgctcttg tgaccgtgct gatccttgcc tgggcgctgc tgcgttatct
     2461 cttttcccgg cctgccgcca tcgccgccta tcgccaggag cgccgccgcc gccaaggctt
     2521 cgacgcgttg acccgtgggc ttctcgccat cggcgtcggc gaccgggccc aggcgcaacg
     2581 ctatgcaggt gtcgcgcgcc gcaatcttcc caacgagccg ctcaccgcgt tgctcaaggc
     2641 gcaagcagcg cagctcaagg gcgaccgcgc ctcggcgcgg cgcgccttcg aggcgatgct
     2701 cgaccgccct gagaccgagc tcttgggact gcgcggactg ttcctcgagg ccaagcgcgg
     2761 caacgacaac ggcgctgctc gcgccctcgc cgagcaggcg gtgcgcaccg atccgcaggc
     2821 cggctggagc gtgaacgcgc tgttcgacat gcaggcgcgc gagggcgatt gggaaggcgc
     2881 gctcgccacg ctcgccgtgg cgcgcaagca tggcaatgtc gagcccgacg cggcgctcag
     2941 gcgccgcgcc gtgctgctca ccgccgaagc gcgcgacctt gaagcctcgc agcctgacaa
     3001 ggcgctcgcg cttgccaccg aagcgttgcg gctggcgccc tcgctcatac cggcggcgga
     3061 gatcgccgga cggctgcttg cctccaaagg agagagccgg caagcaagcc gcctcgtcgc
     3121 ccgcacctgg aagctcgcgc cgcatccgga ccttgccacg gtgtatgcct tcgccaagcc
     3181 tggcgagtcg ccgcgcgacc gcctgaggcg ggtcgagcat cttgcgagcc tcacgcctgg
     3241 cgacatcgaa ggaccgatcg ccatcgccgt ggccgccatc gaagcgcgcg actggcagcg
     3301 ggctgaggac gcgatctcgc cttatctgca cgaccggccg ccggcccgcg tctgcgcctt
     3361 gatggcgcgc atcgaggccg gcgaaggcaa caagggccgc gagcgcgaat ggcttgcccg
     3421 cgccgtgcgc ggcccccgcg accgggcgtg gatcgccgac ggctatattt ccgaccgctg
     3481 gctcccggtc tcgccggtca ccggcgccgt cgacgccttc gaatggaagg cgccggtcga
     3541 cgccatcggc cgcggcgacg cgaccttgct gatcgaggaa agccacgagc cgccggagcc
     3601 tccgcccgcg ttgaaggttg cggccccggt gaaggagacg gccgtgccga aaaagcccga
     3661 gctcgtatcg ccacggccgc tggtcgccgg cgatggcgcc gccccgctga ggccggcgga
     3721 gtcggggcaa atgcgcccgg ccgtccctcc ggtcacaccg gtcccgcccg ccgcgcctcg
     3781 cgagcagccg ccccaagcgc ggccaaaacc ggagttctac gtggcgccgc ccatccccga
     3841 cgatccgggg gtggcgcccg ccgatccgga cgagagccct gcctcgctcg agcgcctgcg
     3901 cgcagcgcaa atccgctaaa ttcgcgggcg cagcgcggcg cccaacttgc catggccggg
     3961 cattggcatt aagcacgggg cgcgcagcca tcttgcctta agcccgcgct tcctcgcgca
     4021 tgccgcaata gctcagctgg tagagcacat cattcgtaat gatggggtcg ggggttcgaa
     4081 tccctcttgc ggcaccaagc ctttttcgcc tttcggaggg ctacaacccc gggtccattc
     4141 ggtccgatcg cgcgaccgga gccggagcgt cctctccgcc acgcccctcc agcgcggcaa
     4201 tggcattctt aagcgtcgcc cgaatgcgct cgatggcggt ggcgtcgatg gccctgggat
     4261 cgaggtagaa gtcgaggccg gtcatgtgtt cgctgaagat ctcaatcttc cgacccgctt
     4321 cgtccagcac accctccacg acatagggaa ccaccatgcg gccaatgccc ttcaccgtga
     4381 tctccggcaa cgcgcgcgcc gccacgatgt cgcgcacgag aacgtaggtg tcgtagctga
     4441 tgacgatatg tcctggctcg gcgatcgatt ggagacgcgc cgcgagatta acctcggcac
     4501 cgatggcggt gtagtccatg cgatgcatgc tgccgaaatt gccgacgttg cagaagccgg
     4561 tgttgacgcc catgcgcacg cggaacggct gttccgtgcc cgcgctacgc catttgacat
     4621 tgagctcggc gagccggcgc tgcatctccg tcgccatgtt gaggcaggct ctcgcgtcct
     4681 ccaccgtgcc cttggtctcg gggtcgccga agaagatcac gagcgcgtcg ccgatgaatt
     4741 tatcgacggt gccgccatga ttatgggcga tcgccgacat ctcggtgaaa tactcgttga
     4801 ggagcagcgt gatctcctct ggctgcaaat gctctgtggt ggaggtgaaa tccttgatat
     4861 cggaaaagaa gatggtgagc tctttgcgct cggtgtggat ggtgacgtcc ctctgaccgc
     4921 tgaagatgct cttgtagatt tgtggcgcga gatagtgcga gatcttcgag gaaatcctgg
     4981 cgagaaagtc attggaggcc tcgagctccc ggttcatgtc cgccaggcgc cgattgagct
     5041 cagcctcttt ctcctgcagc agggtcatgt cataggcctt ctgctgcagg cggcgagtcg
     5101 cgtcgcgctg ctcggtgaca tcgaacaaca cgacccagac ggtgtcgcca tcctgatgaa
     5161 gatgaaggtc ggcggcgcgg ccgcagtcga gctcgatcga gggaacgaaa tagggcgact
     5221 ccacgagcgg cagaagccct tcgaggaaga aggcctgttc gaccgccggt tcaccgaggc
     5281 gcaaggccgc gagcccataa ttgtcgagat gcccgccggc gtcgatcaga gtgagcgagg
     5341 catcgacctg cagataaaca acagcccgct cgtcataggt cagctcttgc agccgctcgg
     5401 ccaggctcac aggcaggcct tccatggttc agctcgaggc gcgggttgcg aggtccttga
     5461 aggcatcctc cacgccttcg ccggttcgcg cgctgcttgg ctgcacccat aagccgttcc
     5521 ggcgcaggcc ctccacctcg gcatcgccga tcgcccactc ctccttgagg tcgctcttgt
     5581 tcaagagcag cacgaacggc agcggaccat attcggtctc gacgcgctgg cggagcgaaa
     5641 gcgcgacgtc gagggtcggg ggccgcgtgc cgtcggcgac caaaacgtat ccggcggcgc
     5701 cgcgcatatt ggtcatgcgg aacgtgttga tgtcgtcctc gccagccagg tcccaaagga
     5761 tgaggttcac gattcgatcg gagaggtcca cggtcttctt gtcgatcttg acgcccaccg
     5821 tggtgaggta ggtttcggag aatatgctct ggacgtagcg cttcaccagg ctggtcttgc
     5881 caaccgagaa gccgccgagc atgcagatct tcttctgcag caccggcgtc atggctcttc
     5941 ctcgaagccg atcgagaatg acacccggcg gttagcggaa cgcgcggccg ctgcggccgc
     6001 aggagtgtcg tcggcaaccg gctcgagctg acccgcgccg cgcacttgaa gcagatcggg
     6061 atcgacgccg cgcttcttca gaagagcgcg gaccgcttcg gcgcgtgcca tgctcagcga
     6121 caggttggag gtgccctgac cttggctgtc agcatgcccg gtcagcgtca tcctggtgac
     6181 tgcgtgcaag gtggaagaca acttcacgag ctcattcagg tcgttcgcga tctcatcgag
     6241 cacctgctcc tggcctgcgg ccggcagcga cttgccgctg tcaaaattga tgctgcgcgc
     6301 ctggatagca tcgcgcaatt tgccgagcgc cccctcgctg atgtcgcgca tggccgacag
     6361 gtctaaaacg ggcgcccctg ccggcagcga gctgccgacc ttgcgggcgc gcgagatcca
     6421 tggcgacggg gccgaccctt gcgccacgat gcggtcgccg tcgaccgcga gcgtcacgcc
     6481 gggcggcggg ttgagggaag cctgcagccg cttgagcacg aattgcgggt cgagcgcctc
     6541 atagggatcg aaatgcgcct cgacccgcgc cggatcgatt cccgcctcgc tgaggagtgc
     6601 gagcgggttg acggcgagcg gatcgcgcaa gcctgacagg aggaacttgt catcgctcgc
     6661 ttccgaccgg gtgatcacga tgccgggctc ggcgcgcagc cgggccacat aggcctgcca
     6721 ttgccgttcg tccacctgcc ggcgctgctc gtccgcctca tcgtcattgc gtaccccggc
     6781 gaggtcgaag ctgggtgccc cggctggcag catctgtgca atagcgtgcg cgcggtcgag
     6841 ccacggccgc ggcgccgaac ctcgcgccac gatgcggttt cctgcgatcg cgagcgtcac
     6901 gctcggcggc ggatcaagcg ttgcggtgag ccgtttcaac accgattgcg ggtcgagccc
     6961 ctgatagggc tcgaaatgcg cctcgacccg cgccggatcg atccccgcct cgccaaggag
     7021 cttgagcggg tcgacggcga gcggatcgcg caaccctgac aggaggaact tgccgtcgcg
     7081 cgcttccgac cgggtgatca cgatgccggg ctcggcccgc agccgtgc
//
LOCUS       DATNAE010000124         2023 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_421543, whole genome shotgun sequence.
ACCESSION   DATNAE010000124 DATNAE010000000
VERSION     DATNAE010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421543"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /gene="frr"
                     /locus_tag="VN518_02680"
     CDS             <1..533
                     /gene="frr"
                     /locus_tag="VN518_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011180549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HXE03508.1"
                     /translation="ERRMRGAFGVLKQEFGGLRTGRASAGILDPITVTAYGNSMPLNQ
                     LATINVPEPRLITVQVWDRSQVGAVERAIRESNLGLNPVVEGQLLRLPVPELNQERRQ
                     EIVKVAHKYAEQARVAVRNVRRDGMEHLKRAEKEHEMGKDEHHSLSTKVQELTDKIIK
                     EIDEALASKEAEIMQV"
     gene            585..1343
                     /locus_tag="VN518_02685"
     CDS             585..1343
                     /locus_tag="VN518_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HXE03509.1"
                     /translation="MTLAVDKLAWQSADRPAVRHVAIIMDGNGRWAAERGLPRAEGHR
                     QGVESVRRTVEAALDLGITHLTLFSFSSENWSRPKHEISDLFGLLRRFIRRDLADLHK
                     NGVRIRVIGTRSGLDADLLRMLDDAVELTKDNVALNLTIAFNYGARDEIARAARRIAE
                     DVAKGTLAPAEVTEARLGCYLDTADVPDPDLLIRTSGELRLSNFLLWQLAYAEFVFID
                     VYWPDFSREQLEAAIAEYQRRSRRFGGTSARSTA"
     gene            1340..>2023
                     /locus_tag="VN518_02690"
     CDS             1340..>2023
                     /locus_tag="VN518_02690"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HXE03510.1"
                     /translation="MTRVKSVSDNSREAKARRRPELAIRLASAVVLAALAIGAILYQP
                     WTFLILVIVGSVLVAWEWGQLTRGNGFDGTALISGVSVATVAILVFLGRADLAFFVLA
                     LAAVAIGLRSVSTGGSVWPLAGLAYAALPTAALLFLRGDPVWGGTAALYLFVVAWATD
                     TASYAAGSLLGGPKLAPRISPKKTWSGFIVGSLTPALVGYAFAGALKETSAVKLSLVS
                     VVLALACQMG"
BASE COUNT          341 a          668 c          641 g          373 t
ORIGIN      
        1 tcgagcgccg catgcgtggc gccttcggcg tcctcaagca ggagttcggc ggcctccgca
       61 ccggccgcgc cagcgccggc atcctcgacc cgattaccgt aaccgcctac ggcaactcca
      121 tgccgctcaa tcagctcgcc acgatcaacg tgcccgaacc ccgcctcatc accgtgcagg
      181 tctgggaccg cagccaggtc ggcgcggtgg agcgtgcgat cagagagtcg aatctggggc
      241 tcaaccccgt ggtcgaaggt caactcctgc ggctccccgt tccggagttg aaccaggagc
      301 ggcgtcagga aatcgtcaag gtcgcgcaca aatacgccga gcaggcgcgg gtggcggtgc
      361 gcaatgtgcg ccgcgacggc atggagcacc tgaagcgcgc cgagaaagag cacgagatgg
      421 gcaaggacga gcaccactcg ctttccacca aggtgcaaga gttaaccgac aagatcatca
      481 aggaaatcga cgaggccctt gctagcaagg aagccgagat tatgcaggtc taattgccca
      541 agatcagagg aagcgccctt acccctaacc cggagcagcc gaacgtgacg cttgcggtgg
      601 ataagcttgc ttggcaatcg gccgacagac cggcggtgcg ccatgtcgcg atcatcatgg
      661 acggcaatgg acgctgggcc gccgagcgcg gcctgccgcg cgccgaaggc catcgtcagg
      721 gcgtggaaag cgtgcggcgc acggtcgagg ccgcgctcga tctcggcatt acccatctca
      781 ccttgttcag cttctcgtcg gaaaattggt cccggcccaa gcatgagatc agcgacctgt
      841 tcggcctgtt gcgccgcttc atccgccgcg atctcgccga cttgcataag aacggcgtaa
      901 ggatccgcgt gatcggcacg cgctcagggc ttgatgccga cttgttgagg atgctcgacg
      961 acgccgtcga actcaccaag gacaatgtcg cgctcaatct cactatcgcc ttcaattacg
     1021 gcgcccgcga cgagatcgcc cgcgcggcgc gccgcatcgc cgaggacgta gcgaagggca
     1081 ccctcgctcc cgccgaggta acggaggcga ggctcgggtg ctatctcgac accgcagatg
     1141 ttccggatcc cgacctcttg atccgcacga gtggcgagct ccgcctgtcg aacttcctgc
     1201 tctggcagct tgcctatgcc gaattcgtgt tcatcgatgt gtattggccg gacttctcgc
     1261 gcgagcagct ggaagcggcg atcgccgaat atcagcggcg cagccggcgt tttggcggca
     1321 cctccgcgag atccaccgcg tgacgcgggt aaaatcggtt tccgacaaca gccgcgaggc
     1381 caaagctcgc cggcggcccg aattggcgat ccgcctggcc tcggccgttg tgctcgccgc
     1441 gctggcgatc ggcgcaatcc tctaccagcc ttggactttc ctcatcctcg tcatagttgg
     1501 cagcgtgctc gtcgcctggg aatgggggca actgactcgc ggcaatggct ttgacggcac
     1561 ggcgctgatc tcgggggtca gcgtcgcgac cgtggccatc ctcgtctttc tcggccgcgc
     1621 cgatcttgct ttcttcgtgc tggccctggc ggcggtggcc atcgggctga ggtctgtttc
     1681 gaccggcggg agcgtctggc cgctcgccgg tctcgcttat gcggccctac cgaccgcggc
     1741 gctcctgttt ctccgcggcg accccgtttg ggggggcacc gccgcgcttt atctttttgt
     1801 tgtagcctgg gccacggaca ccgcctctta tgccgccggg agcctgcttg gtggacccaa
     1861 gctcgcgccg cgaatctcgc cgaagaagac ctggagcggg tttatcgttg gctccctcac
     1921 ccccgctctc gtcggttatg ctttcgccgg ggcgctcaag gagacatcgg cggtgaagct
     1981 ttcgctggtg agcgtcgtgc tcgcgctcgc gtgccaaatg ggc
//
LOCUS       DATNAE010000125         2512 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_1926806, whole genome shotgun sequence.
ACCESSION   DATNAE010000125 DATNAE010000000
VERSION     DATNAE010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1926806"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="VN518_02695"
     CDS             <1..494
                     /locus_tag="VN518_02695"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HXE03511.1"
                     /translation="PDPAIRAELILLLAKAGGWQGMALGQALDLSPARKDFGASDIAE
                     MQALKTGALFRFACEAGAILGRAEPSERAALVAYASYFGQAFQLADDLLDAQGDAKAL
                     GKAVAKDEAQGKATLLALLGPDAAKARLGELLHEAEAALAPFGGRANTLLATVRFAAD
                     RKS"
     gene            507..1223
                     /locus_tag="VN518_02700"
     CDS             507..1223
                     /locus_tag="VN518_02700"
                     /inference="COORDINATES: protein motif:HMM:NF018745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1345 domain-containing protein"
                     /protein_id="HXE03512.1"
                     /translation="MAQRTKATKPRERDLPEWAWITRHLRAQIRFLIALVVGVAVFFF
                     VPIEDLVPRILAGWNAGGWLYLALVAIKMARAEIEGIKRQSTLERESRLVVLVVVIFG
                     SIFTILALFAQLMAIKSEHGMDRTLSISLSVSTIFLSWLLIHTVFALYYAHEFHAEGH
                     AGSRGQGGGLKFPDDSTPDYLDFLYFSFVVGTTAQTSDVAVCSRAMRRVVMLHGILSF
                     FFNTAVIALAVNIAAQLVQG"
     gene            1288..1968
                     /locus_tag="VN518_02705"
     CDS             1288..1968
                     /locus_tag="VN518_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1345 domain-containing protein"
                     /protein_id="HXE03513.1"
                     /translation="MAKPTHGSLPHRLLRLVRTRWRLFLSGAIGIVCVGIFLLTTQWR
                     VVTCMLVGWDIGVGLYLVLCFWMFRVCDRDHIRQQSVLQDEGRYAIPVLTVSAALASL
                     AAILVELHTTAGPATRDPRLLALAIVTILLSWTFIHTIFTLHYAHEYYADRRGNRRGL
                     DFPGDEKPGYWDFVYFSFVIGMTSQVSDVCVTSSNIRQIVAAHGLVSFVYNVALLALT
                     INIAASAL"
     gene            complement(1981..2478)
                     /locus_tag="VN518_02710"
     CDS             complement(1981..2478)
                     /locus_tag="VN518_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03514.1"
                     /translation="MALVVGCSAALAQDEDETGEAADSWIGCWARVYDAVHLAKHPGQ
                     KVTAVTLSIAAREGASGDAPGDYRAKLTALMRDKPDTYSNPGGSRCVASGEKLSCFTD
                     GFFLGKFSLEHAGKNMKLAISGADEHVALVPGIDLSSFVVLSAENPEHTLFLLNPAPA
                     KACAK"
BASE COUNT          406 a          859 c          764 g          483 t
ORIGIN      
        1 atcccgatcc ggccattcgc gccgagctga tcctgttgct cgccaaggcc ggcggctggc
       61 aggggatggc gctcggccaa gcgctcgatc tcagcccggc gcgcaaggac ttcggcgcga
      121 gcgacatcgc cgagatgcag gcgctcaaga ccggcgctct gttccgcttc gcctgcgagg
      181 cgggagccat cctcggccgc gcggaacctt cggagcgtgc cgcacttgtt gcctatgcct
      241 cttatttcgg ccaggccttc cagctcgccg acgatctgct cgatgcgcaa ggcgatgcaa
      301 aggcgctcgg caaggccgtg gccaaggacg aggcccaggg caaggcgacg ctcctcgctc
      361 tgctcggccc cgatgccgcc aaagcgagac tcggcgagct gctgcatgaa gcggaagccg
      421 cccttgcgcc tttcggcgga cgggctaaca ccttgctcgc gaccgtccga ttcgccgcgg
      481 accgcaagag ctgacgagga ggcttgatgg cacagcggac gaaagcgacc aagccgcgcg
      541 agcgggatct gcccgagtgg gcgtggatca cgcgccatct ccgcgcgcaa atccgcttcc
      601 tcatcgctct cgtcgtcggc gtcgccgtct tctttttcgt gccgatcgag gatctggtcc
      661 cgcgcatcct tgccggctgg aacgcgggcg ggtggctcta tctcgcgctt gtcgccatca
      721 agatggcgcg cgccgagatc gaaggcatca agcggcaatc gacgttggag cgggagagcc
      781 gcctcgtcgt cctcgtcgtc gtcatcttcg gctcgatctt caccatactg gcgctgttcg
      841 cccagctcat ggcgatcaaa agcgagcacg gcatggatcg caccctcagc ataagcctgt
      901 cggtctccac catcttcctg tcatggctgc tgatccacac cgtgttcgcg ctctactacg
      961 cgcacgagtt ccacgccgag ggccacgcag gatcgagagg acagggcggc gggctaaaat
     1021 tccccgacga cagcacgccc gactatctcg actttctcta tttctcgttc gtggtgggga
     1081 ccacggcgca aacctcggac gtcgccgttt gctcgcgcgc catgcgccgc gtggtgatgc
     1141 tgcacggcat cttgtcgttc ttcttcaaca cggcggtgat cgctctcgcc gtcaacatcg
     1201 ccgcccagct ggtgcagggc taggccggcc aacctgctac attagcgacg cttgagaaag
     1261 acgccgattc cggctcgaat ggcggccatg gcgaaaccga cgcacggctc cctcccgcac
     1321 cggctcctgc gcctcgtgcg cacacgctgg cgcctgttcc tctccggcgc catcggcatc
     1381 gtttgcgtcg gcattttcct gctgaccacg caatggcggg ttgtcacctg catgctggtg
     1441 ggttgggaca tcggcgtcgg cctctatctt gtgctctgct tttggatgtt cagagtttgc
     1501 gaccgggacc atatccggca gcaatcggtg ctacaggacg aggggcggta cgccatcccg
     1561 gtgctcaccg tcagcgccgc cctggcaagt ctcgccgcca tcctcgtcga gcttcatacg
     1621 actgccgggc ccgccacgcg cgaccctcgg ctgcttgcgc tcgccatcgt cacgatcctg
     1681 ctctcctgga cctttatcca cacgatcttc acgctgcatt atgcgcatga gtactatgcc
     1741 gaccgccgcg gcaatcgccg cgggctcgac tttcccggcg atgaaaagcc gggctattgg
     1801 gacttcgtct atttttcctt cgtcatcggc atgacgtcgc aggtgtcgga cgtttgcgtg
     1861 acatcgagca acatccggca aatcgtcgct gcccacggcc tggtctcttt cgtgtacaac
     1921 gtcgccctgc ttgcgctcac catcaatatc gcggcaagcg cgttgtgagc gcggcgcccg
     1981 ctacttggcg caagccttag caggagcggg attcagcaga aacagcgtgt gctcggggtt
     2041 ctcggccgac agcacgacga agctgctgag atcgatgccc ggcaccagcg ccacatgctc
     2101 gtcggcgccg ctgatggcga gcttcatgtt cttgcccgcg tgctcgagcg agaacttgcc
     2161 gagaaagaat ccatcggtga agcaggacag cttctcgccc gatgccacgc agcgggagcc
     2221 tccgggattg gaataggtgt cgggcttgtc gcgcatcagc gcggtgagct tggcgcgata
     2281 gtcccccggc gcgtcgccgc tcgccccctc gcgcgcggcg atggacagcg tgacggccgt
     2341 gaccttctgg cccggatgct tggcgagatg cacggcgtcg tagacacgcg cccagcagcc
     2401 gatccaggaa tctgccgcct ccccggtctc gtcctcgtct tgtgccagcg ccgcggagca
     2461 gccgacaacc agcgccaaaa ccgcaagcca aactatgcgc attctttact cc
//
LOCUS       DATNAE010000126         2772 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_424094, whole genome shotgun sequence.
ACCESSION   DATNAE010000126 DATNAE010000000
VERSION     DATNAE010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424094"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /gene="rpsO"
                     /locus_tag="VN518_02715"
     CDS             complement(<1..245)
                     /gene="rpsO"
                     /locus_tag="VN518_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006473093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HXE03515.1"
                     /translation="MSISALRKQELIQDYATKPGDTGSPEVQVAILTERITNLTDHFK
                     THNKDNHSRRGLLKMVSQRRRLLDYVKATDEDRYQKL"
     gene            complement(273..1199)
                     /gene="truB"
                     /locus_tag="VN518_02720"
     CDS             complement(273..1199)
                     /gene="truB"
                     /locus_tag="VN518_02720"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HXE03516.1"
                     /translation="MARRRKGSPIHGWLVLDKPQGMTSTQAVSRVRHLYGAQKAGHAG
                     TLDPLATGVLPIAFGEATKTVPFAVDGDKQYRFTVRFGEATDTDDSDGTVIATSDRRP
                     SRAEIEAALPRFVGEISQVPPRYSALKVAGARAYDLAREDQEFELAARPALIESLELT
                     GMPDAEHCVIEARCGKGTYVRALARDLGVALGTLAHVTELRRTRVGPFAEEAAISLAK
                     LEELGHSAAGRDALLGVLKPVETALDDIPALAISGQDAARLKRGQPVLLRGRDAPILK
                     GPVYATSRGSLVAVGEVSQGELRPTRVFNLPG"
     gene            complement(1213..1608)
                     /gene="rbfA"
                     /locus_tag="VN518_02725"
     CDS             complement(1213..1608)
                     /gene="rbfA"
                     /locus_tag="VN518_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HXE03517.1"
                     /translation="MTDRNRGKAPSQRQLKVGELIRHALAEIFARGEIVDDVLDRYSL
                     TVSEVRMTPDLKLATVFVLPLGGEGAEDVVKHLDKHKRFLRGDLARRVNLKFMPELRF
                     KIDTSFASSKRIDELLASPKVARDLDADQ"
     gene            complement(1642..>2772)
                     /gene="infB"
                     /locus_tag="VN518_02730"
     CDS             complement(1642..>2772)
                     /gene="infB"
                     /locus_tag="VN518_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011583238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HXE03518.1"
                     /translation="LLSHEIVVESLGGDTLEIEVSALKKTGLDKLLEAIALQAEVLEL
                     TANPDRPAEGVIVEAKLERGRGPVGTALVQRGTLRLGDLIVAGSAYGRVRALINDLGE
                     QVKEAGPSVPVEVLGFDSAPEAGDQFAVVENEARAREITDYRLRERRKRLAVAGQRGS
                     LEQMMTQLKSAGTKEFTLLVKGDVQGSVEAIVAALDKLGTDEVRARIIHYGVGGITES
                     DVGLASTSGAVILGFNVRANAQAREAAERDGVEIRYYAVIYDLVDEVKQAMSGLLAPT
                     RRETFLGNAEILEVFTISKVGKVAGCRVTEGKVERGAHVRLIRDNVVVHEGKLSTLKR
                     FKDEVKEVVAGQECGMAFEGYQDMRQGDVIECFEVEHVKRTL"
BASE COUNT          452 a          902 c          886 g          532 t
ORIGIN      
        1 atgagcttct gataccggtc ctcgtccgtc gccttgacgt agtcgagtaa ccggcgccgc
       61 tggctaacca ttttcaacag cccacgccgt gaatggttgt ccttgttatg cgtcttgaag
      121 tggtcggtaa gattggtgat ccgttcggtc aggatcgcca cttgcacttc gggtgacccg
      181 gtatctccgg gcttggtggc atagtcttgg ataagctctt gcttgcgcaa agcgctgatc
      241 gacatcggat gtctcctgaa tttgggcgac gcctaacccg gcagattaaa gactcgggta
      301 gggcgcaatt cgccttggct gacctcacca accgccacga gcgaacctcg tgacgtggcg
      361 taaacgggcc ctttgagaat tggagcatcc cggcctctta acagcacggg ttggcctctc
      421 ttgagcctgg ctgcgtcctg cccgctgatg gcgagcgccg ggatgtcgtc cagcgccgtc
      481 tccacgggct tcaacacgcc taaaagcgcg tcgcggccgg cggcactatg tcctaactcc
      541 tccagctttg ccagggaaat tgcggcctct tcggcaaagg gtccgaccct cgttcggcgc
      601 aattccgtga catgggcgag ggtgccgagc gccaccccga ggtcacgggc gagcgcccgc
      661 acataggtgc ctttgccgca gcgggcctcg atgacgcaat gctcggcgtc gggcatgccg
      721 gtaagctcga ggctctcgat cagggccggc cgggcggcaa gctcgaattc ctggtcttcc
      781 ctggcaaggt cgtaggccct ggcgccggcc accttcagcg ccgaatagcg cggtggaacc
      841 tggctgatct cgccgacgaa ccggggcagc gccgcctcga tctcggcccg ggacgggcgc
      901 cggtcgctcg tcgcaatcac cgtgccatcg gaatcatcgg tatcggtggc ttccccgaag
      961 cgcaccgtga agcggtactg cttgtcgccg tcaaccgcga agggcacggt cttggtcgcc
     1021 tcgccgaagg cgatcggcag cactcccgtg gcgaggggat cgagcgtgcc ggcatggcca
     1081 gccttctgcg ccccgtagag atgcctcacc cgcgacacgg cctgcgtcga ggtcatgcct
     1141 tgcggcttgt cgagcacgag ccagccgtga atgggggaac ctttgcgccg ccgcgccatg
     1201 tcgcctctgc tgctactggt cggcgtcgag atcgcgcgcg acctttggcg aggcgagcag
     1261 ctcatcgatg cgcttggagc tcgcgaacga cgtgtcgatc ttgaatctga gctcgggcat
     1321 gaatttcagg ttcacgcgcc gcgcgaggtc accccgcagg aagcgcttgt gcttgtcgag
     1381 atgcttgacc acgtcctcgg cgccttcacc gccgagcggc agcacgaaga cggtggcgag
     1441 cttcaagtcc ggcgtcatgc gcacctcgga cacggtcagc gaatatcgat cgagcacgtc
     1501 gtcgacgatc tcgccgcggg cgaagatttc ggcgagtgcg tgccggatca gctcgccgac
     1561 cttcaattgg cgctgactcg gcgccttacc cctgttgcga tcggtcatgg caatgtctga
     1621 ggccgcttgg ctcaagcggt cttacagcgt gcgcttgacg tgctcgacct cgaagcactc
     1681 gatgacgtcg ccctggcgca tatcctggta gccttcgaac gccatgccgc attcctggcc
     1741 ggcaacgacc tctttcactt catccttgaa gcgcttgagc gtcgacagct tgccttcgtg
     1801 caccacgacg ttgtcgcgaa tgagccggac atgggcgccg cgctcgacct tgccttcggt
     1861 gacgcggcag ccggcgacct tgccgacctt ggagatggtg aacacctcca ggatctcggc
     1921 attgccgagg aaggtctcgc gcctggtcgg tgcgagcagg ccggacatgg cttgcttcac
     1981 ctcgtcgacg aggtcgtaga tcacggcgta gtagcggatc tctactccgt cgcgctcggc
     2041 agcctcgcgg gcctgcgcgt tggcgcgcac gttgaagccg aggattacgg cgccggaggt
     2101 ggaagcaaga cccacgtcgg attcggtgat gccgccgacg ccataatgaa tgatgcgggc
     2161 gcgcacctcg tcggtgccga gcttgtcgag agcggcgacg atcgcctcca ccgagccctg
     2221 cacgtcgcct ttgacgagaa gcgtgaactc cttggtcccg gccgatttca gctgggtcat
     2281 catctgctcg agagagccgc gctgaccggc aacggcaagg cgtttccgcc gctcgcgcaa
     2341 gcggtaatcg gtgatttccc gggcgcgggc ctcgttctcc accaccgcga actggtctcc
     2401 ggcttccggc gctgaatcga agcccaagac ctcgaccggc accgaggggc cagcttcctt
     2461 gacctgctcg ccgaggtcgt tgatcagcgc gcgcacgcgg ccataggccg agcctgccac
     2521 gatgagatcg ccaagacgaa gcgtgccgcg ctggacgaga gcggtgccaa cggggccacg
     2581 gccccgctcg agcttggctt cgacgatgac gccctcggcg gggcggtccg gattggcggt
     2641 aagctccagc acctcggcct gcagtgcgat cgcctcaagc agtttgtcga gtccggtctt
     2701 cttcagcgcc gagacttcga tctcgagcgt atcgccgccg aggctctcga ccacgatttc
     2761 atggctgagc ag
//
LOCUS       DATNAE010000127         2646 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2229404, whole genome shotgun sequence.
ACCESSION   DATNAE010000127 DATNAE010000000
VERSION     DATNAE010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2229404"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="VN518_02735"
     CDS             <1..401
                     /locus_tag="VN518_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03519.1"
                     /translation="ASRFDIGVVIAAGMTIGTIFTLFVTPAVYTFLAKDYQKARAREA
                     EHGLPASAEAAVPAPAMAAAPAATAPHDPQAIANALAKVANETPEEEGHSAEVLAFSS
                     AAAAASPEPERPRKGRRKPPRPKPLTPAAE"
     gene            468..2477
                     /locus_tag="VN518_02740"
     CDS             468..2477
                     /locus_tag="VN518_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HXE03520.1"
                     /translation="MFAKLLIANRGEIACRIMRTARRLGILTVAVYSDADRDALHVDM
                     ADEAVRIGPAAASQSYLDIAKIVAAAKETGADAIHPGYGFLSENAAFARALAKAGIIF
                     VGPNVKAIEAMGDKIESKKLAAKAKVSTVPGHPGVIKDAKEAAKIADEIGYPVMIKAS
                     AGGGGKGMRIAYSRKEVEEGFNSSKSEAKSSFGDDRVFVEKYIEEPRHIEIQVLGDKH
                     GNVIHLGERECSIQRRNQKVVEEAPSPFIDDETRAAMGAEAVSLAKAVGYDTAGTVEF
                     IVDKDRNFYFLEMNTRLQVEHPVTELVTGLDLVEAMLRVAAGEKLKLKQKDVKLEGSA
                     IEARIYAEDPVRNFMPSTGRLIRYRPPREGTTVDGITTRLDSGVVEGGEISVYYDPMI
                     AKLITHAPTRLQAIEHMGEALDAFAIDGFRHNIPFLSVLMRNNRWREGRLCTKFIAEE
                     FPGGFKPPLPTGDERDAIVAIAATIDHMSNQRRRVITQQMHGEPVQFAEVRIIEIAGA
                     AERVTVAGDVEGPFTVIFEGGGKTINLASNWWFGDPVWKGTIDGKRAFVQIRPILNGY
                     DIIYEGVEVPVRVLTEREAELAALMLVKEKADTSKFLLCPMPGMVVSLAVDVGQDVRA
                     GDTLCIVEAMKMENVLKAERDGKVSKVLAKPGDSLNVDAVILEFA"
BASE COUNT          511 a          836 c          866 g          433 t
ORIGIN      
        1 ccgcgagccg cttcgacatc ggcgtggtga tcgccgccgg catgaccatc ggcacgatct
       61 tcaccctgtt cgtcacgccg gcggtctaca ccttcctcgc caaggactat caaaaggcga
      121 gggcgcgcga ggccgaacac ggcctgccgg ctagtgccga ggctgccgtt cccgccccgg
      181 cgatggccgc agcgcctgcg gcgacggctc cccacgatcc tcaggccatc gccaatgcct
      241 tggccaaggt cgcgaacgaa acgccagagg aggagggcca ttctgctgag gtgctcgcct
      301 tcagctcggc tgcggcggcg gcctcgccag agccggagag gccccgcaag ggccgtcgga
      361 aaccgcctag gccgaagccg ctgacccccg cggcagaata gtcactagtt tactcgcccc
      421 cagcgagcct ctagaagtgc ccggccagag ctggggatcc ctccaccatg ttcgccaagc
      481 tgctcatcgc caatcggggt gaaatcgcct gccgcatcat gcggacggcg cggcggctcg
      541 gcatcctcac ggtcgccgtc tattccgacg ccgaccgcga cgcgcttcac gtggacatgg
      601 cggacgaggc ggtgcgtatc ggccccgccg ccgcgagcca gtcctatctc gacatcgcca
      661 agatcgtcgc tgccgccaag gagaccggcg ccgatgccat ccatccgggc tacggcttcc
      721 tttcggagaa cgccgccttc gcccgggcgc tggccaaggc cggcatcatt ttcgtcgggc
      781 cgaacgtcaa ggcgatcgag gccatgggcg acaagatcga gtcgaagaag cttgccgcca
      841 aggcaaaggt ctcgaccgtg ccaggccatc ccggggtgat caaggacgcc aaagaggcgg
      901 ccaagattgc cgacgagatc ggctatccgg tgatgatcaa ggcgtcggcg ggcggcggtg
      961 gcaagggcat gcgcatcgcc tatagccgca aagaggtcga ggaaggcttc aactcgtcga
     1021 agtcggaagc caagtcgagc ttcggcgacg accgcgtgtt cgtcgagaaa tatatcgagg
     1081 agccgcgcca tatcgaaatt caggtgctcg gcgacaagca cggcaatgtc atccatctcg
     1141 gcgagcgtga gtgctcgatc cagcgccgca atcagaaggt ggtggaggag gcgccgagcc
     1201 cgttcatcga cgacgagacc cgcgccgcga tgggcgctga agccgtgtcg ctcgccaagg
     1261 ccgttggcta cgacaccgcg ggcaccgtgg agttcatcgt cgacaaggac cgcaacttct
     1321 atttcctcga gatgaacacg cggcttcagg tcgagcatcc ggtgaccgag ctcgtcaccg
     1381 ggctcgatct tgtcgaggcc atgctcaggg tcgccgccgg cgagaagctc aagctcaagc
     1441 agaaggacgt gaagctcgaa ggctcggcga tcgaggcgcg catctacgcc gaggacccgg
     1501 tgcggaactt catgccgtcc accgggcgcc tcatccgcta tcggccgccg cgcgagggca
     1561 ccacggtcga cggcatcacc accaggctcg attcgggcgt cgtcgagggt ggcgagatct
     1621 cggtctatta cgacccgatg atcgccaagc tgatcacgca cgcgccgacg aggctacagg
     1681 ccatcgagca tatgggcgaa gcgcttgacg ccttcgccat cgacggcttc cgccacaaca
     1741 ttcccttcct ctccgtgctg atgcgcaaca acaggtggag ggaagggcgg ctctgcacca
     1801 aattcatcgc cgaggaattt cccggaggct tcaagcctcc gctgcccacg ggcgatgagc
     1861 gtgacgcgat cgtggccatc gccgccacca tcgaccatat gagcaatcag cgccgccgcg
     1921 tcatcaccca gcaaatgcac ggcgagccgg tgcagttcgc cgaagtgcgc atcatagaga
     1981 tcgccggtgc cgccgagcgg gtcacggtcg cgggcgatgt cgaggggccg ttcacggtca
     2041 tcttcgaggg aggggggaag acgatcaatc tcgcgtcgaa ttggtggttc ggcgatccag
     2101 tgtggaaggg gaccatcgac gggaaacgcg ccttcgtgca gatccggccg atcctcaacg
     2161 gctacgacat catttatgag ggcgtcgagg ttccggtcag ggtgctgacc gagcgcgagg
     2221 ccgagctcgc ggcgctgatg ctggtgaagg agaaggcaga tacctcgaag ttcctgcttt
     2281 gcccgatgcc gggcatggtg gtgtcactcg cggttgatgt cgggcaggac gttcgggcag
     2341 gcgacacgct ctgcatcgtc gaggcgatga agatggagaa cgtgctgaaa gccgagcgcg
     2401 acggcaaggt ctccaaggtt ctggccaagc ccggcgacag cctcaatgtc gacgccgtga
     2461 tcctcgaatt cgcttgagtc gcgtccttgg ttcactgtca ttgcccggct ggaccgggca
     2521 atccagtaaa ctcggaggat gtctgaaatc ggtgtttact gaatgcccgc ttttgcgggc
     2581 attcagtctg gtgcgtgcat gaaagagaaa agcgagccga gacgcacggt cacggtgagg
     2641 atcgag
//
LOCUS       DATNAE010000128         3606 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_1025844, whole genome shotgun sequence.
ACCESSION   DATNAE010000128 DATNAE010000000
VERSION     DATNAE010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1025844"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(199..1179)
                     /locus_tag="VN518_02745"
     CDS             complement(199..1179)
                     /locus_tag="VN518_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018856974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXE03521.1"
                     /translation="MPSKLRLTIATTDYDHVRDLRFGLVQPEGIEVTYLCMGIHEIFA
                     RFAQGREWEVSELSFAKFIAQATEPDADIIGLPVFLARRFRFQAFIVNKKGPVKTPQD
                     LKGKRIGIPEWAQTATVYTRGWLQHEVGVPLDSVEWVQAGENEAGREEKVESTLPKGV
                     RLTRIADKSLSEMLVSGEIDCMMSAGRPVALRNPDIGRLMPNYQEFDREFFERTRVYP
                     IMHIVAMRKSVLEKHRWAARNLFNAFEEAKDRAVARALRGTPIPVPWLSEYAREMQGE
                     FGEDLYPYGIEPNRPTLEHFLRYAYEQGVAKKHVKPEEIFPEGIMTSVKI"
     gene            complement(1190..2182)
                     /locus_tag="VN518_02750"
     CDS             complement(1190..2182)
                     /locus_tag="VN518_02750"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXE03522.1"
                     /translation="MNWDEDLSPSPEELARSGSLEELYARLARIPIGPGWNKPTPSLW
                     DAPRKSLVPYRWRYVQGRGALEAAGRLINTELAERRNLILANPAGDGYATTRSMITAY
                     QMIMPGERARSHRHMPNALRLVLESEPGCYTVVNGKRLEMEPGDVLLTPNWSWHGHGN
                     ESESNGYWADYLDVPLVHLLEPMFFEPHPKGFELDAPIVHDDPNIFRWQDTLDRLDAA
                     PATPASRYATEIALGDPALETMGLSMMRLEPGTSTERHRTTAGNIYTVAHGSGVTEID
                     GQSFDWQHGDVIAVPAWLPHSHRANEVSVLFRVSDEPAMQKLGFLRSETASSPH"
     gene            complement(2179..>3606)
                     /locus_tag="VN518_02755"
     CDS             complement(2179..>3606)
                     /locus_tag="VN518_02755"
                     /inference="COORDINATES: protein motif:HMM:NF004780.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HXE03523.1"
                     /translation="VSSISGWEFGREPFPTLAGERPPPQSPQHRERCPQDMFDPILAR
                     FARSFEDVTLRYDCELLGFEERADGVLATLKNSSTGREEQITCAYLVGCDGAASTVRQ
                     TLGIGMSGNPVLTYTTNVIFRCQGWLDLHDKGPAYRFIMIGPEGTYATIVAINGRDRF
                     RFSIVGDETKRSYDEAEVRALILKAVGKAFDFEILSIVPWIRRELVADSYGSARALIA
                     GDAAHLTSPTGAFGMNMGIQDAVDLGWKLEGAVRGWGGPDLLASYETERRPVAIRNVR
                     EASGNLRRMLSPRDKKPPPEAFAPGPQGDAARKEFGQLFTDIMHREWFTLGIHLGYRY
                     EGSPVIVPDGSLEPPDDPEHYVQTARPGHRAPHVWLDDGRSTLDLFGRGFVLLRLGAA
                     PPEGARLIAAAAAAKLPLMVETLAEPSLLAAYEKRLVLVRPDGHVAWRGDAEPADAAR
                     VIATVTGRRGAAPARAQGQIAEEAR"
BASE COUNT          580 a         1154 c         1261 g          611 t
ORIGIN      
        1 gcccgaccct cgaggccttc atgacctatc tccacgagca gggcatgatc gccaagcctt
       61 tcccgatcga ggacatcttc gtgccggtcg ggagtccagc cgccgcgcat gcttagagtc
      121 cctctcccga cgggagaggg agggacccgg cgcgtagcgc tgggagggtg aggggaaaat
      181 cggtgaggcg caggttcttc agatcttcac cgaggtcatg attccttccg ggaagatctc
      241 ctccggcttc acatgcttct tggcgacgcc ctgctcgtag gcgtagcgca ggaaatgctc
      301 gagggtcggg cggttgggct cgatgccgta ggggtagaga tcctcgccga actcgccttg
      361 catctcgcgc gcatattcgc tgagccaggg caccgggatc ggcgtgccgc gcagggcgcg
      421 ggcgacggcg cggtccttgg cctcctcgaa ggcgttgaag aggttgcgcg ccgcccagcg
      481 atgcttttcg agcaccgact tgcgcatggc gacgatgtgc atgatcggat agacccgcgt
      541 gcgctcgaag aattcgcggt cgaattcctg gtagttgggc atcaggcggc cgatatcggg
      601 gttgcgcagg gcgacggggc gccccgccga catcatgcag tcgatctcgc ccgagaccag
      661 catctcgctc aggctcttgt cggcgatacg ggtgagacgc acgcccttgg gcagagtgga
      721 ttcgaccttc tcctcgcgcc ccgcctcgtt ctcgccggcc tgcacccatt cgaccgaatc
      781 gaggggcacc cccacctcgt gctgcagcca gccgcgcgta tagacggtgg cggtctgcgc
      841 ccattcggga atgccgatgc gcttgccttt gagatcctgc ggcgtcttca ccggcccctt
      901 cttgttgacg atgaaggcct ggaagcggaa gcggcgggcg agaaagaccg gcaggccgat
      961 gatgtcggca tcgggctcgg tcgcctgggc gatgaacttg gcgaagctga gctccgacac
     1021 ctcccattcg cgaccctgcg cgaagcgcgc gaagatctca tggatgccca tgcagagata
     1081 ggtgacctcg ataccctcgg gctggacgag gccgaagcgc aggtcgcgga catggtcgta
     1141 atcggtggtg gcgatcgtca ggcgaagctt ggatggcatt gggctgcttt caatgaggag
     1201 aagaggcggt ctcgctgcgc aggaatccga gcttctgcat cgccggctcg tcgctgacgc
     1261 ggaacagcac cgagacctcg ttggcgcgat gcgaatgagg aagccaggcg ggtaccgcga
     1321 tgacatcgcc atgctgccag tcgaagcttt gcccgtcgat ctcggtcacg ccgctgccat
     1381 gcgccacggt gtagatgttg ccggcggtgg tgcggtggcg ctcggtgctc gtccccggct
     1441 cgagccgcat catggagaga cccatcgtct cgagcgccgg atcgccgagc gcgatctcgg
     1501 tggcgtagcg gcttgccggc gttgccggtg cggcatcgag gcggtcgagc gtgtcctgcc
     1561 agcggaagat gttgggatcg tcatggacga tcggcgcgtc gagctcgaag cccttgggat
     1621 gcggctcgaa gaacatgggc tcgagcaggt gcaccagcgg cacgtcgaga taatcggccc
     1681 aatagccgtt gctctcgctc tcgttgccat gcccgtgcca ggaccagttg ggggtgagca
     1741 gcacgtcgcc cggctccatc tcgagccgct tgccattgac caccgtgtag cagccgggct
     1801 cggattcgag cacgaggcgc agcgcgttgg gcatgtggcg gtgcgagcgc gcccgctcgc
     1861 ccggcatgat catctgataa gcggtgatca tgctgcgggt cgtggcatag ccgtcgccgg
     1921 ccggattggc gaggatgagg ttgcggcgct cggcgagctc ggtattgatc agccgcccgg
     1981 cggcttcgag cgcgccgcgc ccctgcacat agcgccagcg atagggcacg agcgatttgc
     2041 gcggcgcgtc ccacagcgag ggcgtcggct tgttccagcc cggaccgatc ggaatgcggg
     2101 cgaggcgcgc atagagctcc tcgagcgagc cggaccgggc aagctcctcg gggctgggcg
     2161 aaagatcctc gtcccaattc atcgcgcctc ctcggcgatc tgcccctgcg ctcgtgccgg
     2221 cgccgcgccg cgccggccgg tcacggtggc gatcacgcgc gccgcatcgg caggctcggc
     2281 atcgccgcgc cacgcgacat ggccgtcggg acgcaccagt accaaacgct tttcataggc
     2341 ggcgagcagg ctgggctcgg cgagagtctc gaccatcagg ggcagcttgg cggctgccgc
     2401 cgcggcgatc aaccgcgccc cttcaggcgg cgccgcgccg aggcgcagca gcacgaagcc
     2461 gcggccgaag agatcgaggg tcgaacgccc gtcatcgagc cagacatgcg gcgcgcgatg
     2521 gccgggccgc gcggtctgca cataatgctc gggatcgtcg ggcggctcga ggctgccgtc
     2581 gggcacgatc accggcgagc cctcatagcg atagcccaga tggatgccga gggtgaacca
     2641 ttcgcgatgc atgatgtcgg tgaaaagctg cccgaattcc tttcgcgcgg cgtcgccttg
     2701 cggcccgggc gcgaacgctt cgggcggcgg cttcttgtcc cgggggctca gcatgcgcct
     2761 gagattgccg ctcgcctcgc gcacattgcg gatggcgacc gggcggcgct cggtctcgta
     2821 gctggcgagg agatcgggcc cgccccagcc gcgcaccgca ccctcgagct tccaaccgag
     2881 atcgaccgcg tcctggatgc ccatgttcat gccgaaggcg ccggtgggcg aggtgagatg
     2941 tgccgcgtcg ccggcgatca gggcgcgcgc gctgccatag gagtcggcga ccagctcacg
     3001 gcgaatccag ggcacgatgc tcaggatctc gaagtcgaac gctttgccga ccgccttcag
     3061 gatcagcgcc cgcacctccg cctcgtcata gctgcgcttg gtctcgtcgc cgacgatcga
     3121 gaagcggaag cggtcgcgac cgttgatcgc gacgatggtg gcgtaggtgc cttccggccc
     3181 gatcatgatg aaacggtagg ctggaccctt gtcgtggaga tcgagccaac cctggcagcg
     3241 gaagatgacg ttggtcgtgt aggtcaggac cggattgccg ctcatgccga tgccgagcgt
     3301 ctggcgcacc gtgctcgcgg cgccgtcgca gccgacgaga taggcgcaag taatctgctc
     3361 ctcacggcct gtgctgctgt tcttcagcgt tgcgaggaca ccgtcggcac gctcctcgaa
     3421 gccgagcagc tcgcaatcgt agcgcagcgt cacatcctcg aaggagcgcg cgaagcgcgc
     3481 gaggatggga tcgaacatgt cctgcgggca gcgctcgcga tgctgcgggc tctgcggcgg
     3541 cggccgctcg cctgcgaggg tcgggaaggg ctcgcggccg aactcccagc cagatatcga
     3601 tgaaac
//
LOCUS       DATNAE010000129         9725 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3434827, whole genome shotgun sequence.
ACCESSION   DATNAE010000129 DATNAE010000000
VERSION     DATNAE010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 9725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9725
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3434827"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VN518_02760"
     CDS             <1..240
                     /locus_tag="VN518_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03524.1"
                     /translation="EQIANLSDDEIAKLDETLGLDGRIENDDWTSKAVALMAETTADE
                     VPAGEDEAEEAPAAAKAPKAAKAKEPKPAKKGKKS"
     gene            415..1821
                     /gene="leuC"
                     /locus_tag="VN518_02765"
     CDS             415..1821
                     /gene="leuC"
                     /locus_tag="VN518_02765"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HXE03525.1"
                     /translation="MAKARTLYDKIWDEHLVHEAPDGTCLIYIDRHLVHEVTSPQAFE
                     GLRMAGRKVRSPEKTLAVVDHNVPTSDRSKGIDDPESALQVQTLAENARDFGIEYYHE
                     RDRRQGIVHVVGPEQGFTLPGTTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTL
                     IQKKARNMRVRVDGALKDGVTAKDIILAIIGEIGTAGGTGHVIEYAGEAIQALSMEGR
                     MTICNMSIEGGARAGLIAPDDKTFDYIKGRPRAPKAGAYETALRHWQTLYSDDEASFD
                     REIVLHAEKLPPLVTWGTSPEQVISVTGRVPRPEEIADETKRKAAVRSLDYMGLQGGE
                     KITDLTLDRVFIGSCTNGRIEDLREVARIVDGKRVHESVSAMIVLGSGLVKEQAEAEG
                     LDKIFKAAGFDWREPGCSMCLGMNPDQLAPRERCASTSNRNFEGRQGRLGRTHLVSPV
                     MAAAAALAGRFIDIRDFH"
     gene            1825..2619
                     /locus_tag="VN518_02770"
     CDS             1825..2619
                     /locus_tag="VN518_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXE03526.1"
                     /translation="MTKSLAQQRFGRAAADYATSEVHAKGASLQRLVDLTEPKPHWRV
                     LDVATGAGHTALAFAPHVAKVTATDITEEMLGQTRKLAADRGLANVKALTANAEDLPF
                     PDASFDLVVCRLAAHHFRKISVFVSEAFRVLMPGGTIGIVDNVAPDAAIVPERTAEEV
                     RHCAALFNAFKKLSDPSHVRCLGLLEWQTLLGRAGFTVTHAECLDKEIEFGTWVQRMR
                     SSEATTARLTEMLRDEPLRSFLKPRETASGPDFTLTEGIILGRKPR"
     gene            2600..3028
                     /locus_tag="VN518_02775"
     CDS             2600..3028
                     /locus_tag="VN518_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HXE03527.1"
                     /translation="MAASRARGLDWQGAQAPSLAELEAIADQAFKRLPRRFRDLCKDV
                     VVRVEDFPTDEVLDALDAESPFDLLGLYHGVDMARQSVMDPAAMPEMVFLYRRPILDY
                     WAEHEETLGHIVTHVLVHEIGHHFGLSDADMHRIEEEAAA"
     gene            complement(3136..3735)
                     /locus_tag="VN518_02780"
     CDS             complement(3136..3735)
                     /locus_tag="VN518_02780"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXE03528.1"
                     /translation="MAVLVGFVLAVPRAHAVEEMRQFNKDDTKTGRTKACPKGERYSE
                     RKRDCVKTSCGTGQVWSGGADACLDGNSASLTDEDLYLAAGDLVLEARYVDALPLLFR
                     IRDRNDPQVLNLIGYSTRKLGDVDKGIDYYHQALAIDPNYTKARQYLGEAYLLKDDVA
                     KAKEQLMEIADRCGGPCADYELLVAAIAARVTGEANGDW"
     gene            complement(3782..4408)
                     /locus_tag="VN518_02785"
     CDS             complement(3782..4408)
                     /locus_tag="VN518_02785"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXE03529.1"
                     /translation="MAKSIGFRTALGAGAILLLLALAMPQAFAVEDTDDSSSTKASKS
                     CPSGKRYSEKKHGCVSTSCGTGQIWSSGVGSCVDGNSASLTDDDLYIAGRELGQQAHY
                     AEALKLFFRIKNREQARVLGSIGYSTRKLGDIDKGIGYYHQALAIDPGYTKVREYLGE
                     AYLQKGDVEGAKAQLMEIADRCGGPCDDYELLVKAIAAHVTGEQTVDW"
     gene            4622..5119
                     /locus_tag="VN518_02790"
     CDS             4622..5119
                     /locus_tag="VN518_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03530.1"
                     /translation="MGSAARLRGIIVLFLALAVAGPEPQAVAIEKDKLVPAGRLVISG
                     RRLSCGSTQTLLRDFEGFAVSSTVIMLNMQALDPLPRQVQWLIYYHECGHILYGPSET
                     SADCYAVRRARREGWLNDQALNDICAVFNIAGHGPVHPDPQARCDQLRQCFGKAGVTA
                     REGDK"
     gene            5198..5815
                     /locus_tag="VN518_02795"
     CDS             5198..5815
                     /locus_tag="VN518_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03531.1"
                     /translation="MRIGGALFGVLVCGSLAFQPSPARAQEHTFLDGPSGPTIDDYLK
                     QAGPDVKLVPQGSLKIDGRTVNCGKRPTVLNSNFDSWGGAFPGFVILNTKKITGLSTA
                     VKLYVYSHECGHQFEGPDETKADLFAIRRGVKWGWLDAVGMEEICTFISTLKGDAVHP
                     PGPQRCETMRNYYKQLIESGTTQSASTPAAEPVSKQKAQALAPQQ"
     gene            5891..6496
                     /gene="leuD"
                     /locus_tag="VN518_02800"
     CDS             5891..6496
                     /gene="leuD"
                     /locus_tag="VN518_02800"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018010071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HXE03532.1"
                     /translation="MEKFTTLTGIAAPLPMINVDTDMIIPKQYLKTIKRTGLGTGLFA
                     ELRYDEEGKPHPHFVLHKPPYTEAKILITGENFGCGSSREHAPWALLDFGFRCVIAPS
                     FADIFYNNCFKNGILPIKLPQSEIDKLLDDASRGANASITVDLENQEIRGPDGGVIHF
                     DIDPFLKRCLMEGLDDIGLTLEKAEHIDSFETESRTRRPWA"
     gene            complement(6585..6962)
                     /locus_tag="VN518_02805"
     CDS             complement(6585..6962)
                     /locus_tag="VN518_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03533.1"
                     /translation="MAAPTAAEDAKVLALGLTDHEATQDEIEKGEALKAPHFNTPAIA
                     YVLAANLKKGDIVEIALVNEDRSLLHNAQTLAEDQAKLLLQAGKRGVPAGGWPEGSYH
                     ATLTITRDGKTLVEQSSQPISFE"
     gene            7058..8164
                     /gene="leuB"
                     /locus_tag="VN518_02810"
     CDS             7058..8164
                     /gene="leuB"
                     /locus_tag="VN518_02810"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HXE03534.1"
                     /translation="MSYKLLLLPGDGIGPEVMAEVSRVIGWVNARGAAKIETETDLVG
                     GCAYDAHGEAISDATMRRASAADAVMLGAVGGPKWDGVPYDQRPEAGLLRLRKDLELF
                     ANLRPAICYPALADASSLKRDRVDGLDIMIVRELTGGVYFGEPKEMKDLGNGQKRAVD
                     TQVYETYEIERIAAVAFELARLRRNQVHSAEKRNVMKTGVLWNEVVTRLHKERYSDVT
                     LHHILADNCAMQLVRAPKQFDVIVTDNLFGDMLSDEASMLTGSLGMLPSASLGAADER
                     GHRRALYEPVHGSAPDIAGRGIANPIATIASFAMCLRYSFNLGETADRIQKAIAFVLD
                     EGYRTADIAQENCRTIGTAEMGTAIIAALDRMAA"
     gene            8338..9030
                     /locus_tag="VN518_02815"
     CDS             8338..9030
                     /locus_tag="VN518_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03535.1"
                     /translation="MALLRTAFVAASIALAMSLPPLAEEPGASTSPSEAAPPAGDSGK
                     DDLTAEERAEKQARKACKVKICDILATKNPQGDDVACEIVKTWREQDITKMLGGRFNW
                     TWGKAVCQSKLEVKREALVKAMTEANYDVVLPEQKVRCTLAQKSEGEPYAVDISIAPK
                     VAFESGKAVSAKLNWGEAKAPMLAYALIYAGTGLDNSANILGPEVTRMVNEFTTKKCA
                     KLKDDTPRSTGN"
     gene            9047..9430
                     /locus_tag="VN518_02820"
     CDS             9047..9430
                     /locus_tag="VN518_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HXE03536.1"
                     /translation="MYYSAPYFITIGRVLMAIMFIQGGIEKMFGYAGAQGYMEAHGVP
                     GVLLPLVILTEAGGGLCVLLGLFTRWWAIALAGFCVLAALFFHWHPEDQMQMINFMKN
                     ITIAGGFLVLAGAGPGALALDNRLT"
     gene            9475..>9725
                     /locus_tag="VN518_02825"
     CDS             9475..>9725
                     /locus_tag="VN518_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03537.1"
                     /translation="MTDAFIEQLRPLGFEKYASRLTRIVGNATQVVVGGDQVRRGLAP
                     DETGAVIVGDMDSAAARLSDDGTFVIDRLRELGEAGLEA"
BASE COUNT         1828 a         3160 c         3002 g         1735 t
ORIGIN      
        1 gagcagatcg ccaatctttc cgacgacgag atcgcgaaac tcgacgagac gcttggcctc
       61 gatggtcgga tcgagaatga cgactggacg tcgaaggccg tcgccctgat ggccgagacc
      121 accgccgacg aggttccagc cggtgaggac gaagccgagg aggctccggc cgcggccaag
      181 gcgccgaagg ccgcgaaagc caaggagccg aagccagcca agaagggcaa gaagagctag
      241 cttccttcaa ttcagagctg tcatgcccgc atccagtact ccgtgagctt gataatttcc
      301 ttcggtggct actggatcgc ccggtcaagc cgggcgatga caatcccagg ggatttgtaa
      361 accccctacg gagacgctat ctcagggcca gaaaagcctt gagacgacgg aaaaatggcc
      421 aaagcgcgca cgctgtacga caagatctgg gacgagcacc tggtgcacga ggcgcctgac
      481 ggcacctgcc tgatctatat cgaccgccac ctcgtgcatg aggtgacgag cccgcaggcc
      541 ttcgagggcc tgcgcatggc cgggcggaag gtgcgcagcc ctgagaagac gctcgccgtg
      601 gtcgatcaca atgtgccgac ctcggatcgc tcaaaaggca tcgacgatcc agagtcggct
      661 ctccaggttc aaacgctggc cgagaacgcg cgcgacttcg gcatcgagta ttaccatgag
      721 cgcgaccgcc gccagggcat cgtgcatgtg gtcggtcccg agcagggctt cacgctgccc
      781 ggcaccacca tcgtctgcgg cgattcgcac acctccacgc atggcgcctt cggcgcgctc
      841 gcccacggca tcggcacgag cgaggtcgag cacgtgctcg ccacccagac tctgatccag
      901 aagaaggcga ggaacatgcg cgtgcgcgtc gacggcgcgc tgaaggacgg cgtcaccgcc
      961 aaggacatca tcctcgccat catcggcgag atcggcaccg ccggcggcac cggccatgtc
     1021 atcgaatatg ccggcgaggc aattcaggcg ctctccatgg aagggcgcat gaccatctgc
     1081 aacatgtcga tcgagggcgg cgcccgcgcc ggcctgatcg cgcccgacga caagaccttc
     1141 gactacatca aaggccgccc ccgcgcgcca aaggccggag cctacgagac cgcgctccgc
     1201 cattggcaga cgctctattc cgacgatgag gcgtccttcg accgcgagat cgtgctgcac
     1261 gccgagaagc taccgccgct cgtcacttgg ggcacgagcc cggagcaggt gatctcggtc
     1321 acagggcgcg tgccgcggcc ggaggagatt gccgatgaga ccaagcggaa agccgctgtc
     1381 cgctcgctcg actatatggg tctacagggc ggcgagaaga tcaccgacct gacgctcgac
     1441 cgcgtgttca tcggctcctg caccaatggc cgcatcgagg atttgcgcga ggtggcgagg
     1501 atcgtcgacg gcaagcgcgt gcatgagagc gtgtcggcca tgatcgtgct cggctcaggg
     1561 ctcgtgaagg agcaggccga agccgaaggc ctcgacaaga tcttcaaggc tgcgggcttc
     1621 gactggcgcg agcctggttg ctcgatgtgc ctcggcatga accccgatca gcttgcgccc
     1681 cgcgagcgct gcgcctcgac ctcgaaccgc aatttcgagg gccgccaggg caggctcggc
     1741 cgcacccatc tcgtctcgcc ggtgatggcg gcggcggctg cgcttgccgg ccgtttcatc
     1801 gacatccgcg actttcattg agctttgacg aaatccctgg cccagcagag atttggcaga
     1861 gcggcggcgg actacgccac ctccgaggtg cacgccaagg gcgcgagcct tcagcggctc
     1921 gtcgatctca ccgagccaaa gccgcattgg cgcgtgctcg acgtggcgac cggcgccggc
     1981 cacaccgctc tcgcctttgc gcctcacgtc gccaaggtca ctgccaccga catcaccgag
     2041 gaaatgctcg gccagacgcg caagcttgcg gccgacagag gtttggccaa tgtcaaagct
     2101 ctgaccgcca atgccgagga tcttcctttt cccgacgcga gcttcgacct cgtcgtctgc
     2161 cgcctcgccg cgcatcattt tcgcaagata agcgtttttg tcagcgaggc gttccgcgtg
     2221 ctgatgcctg gcggcacgat cgggatcgtc gacaacgtcg cgcctgacgc cgccatcgtt
     2281 cccgagcgca cggcagagga agtgcgccat tgcgccgcct tgttcaacgc cttcaagaaa
     2341 ctttccgacc cgagccatgt ccgctgcctt ggccttctcg aatggcagac gctgcttggg
     2401 cgtgcaggct tcaccgtcac gcacgccgag tgcctggaca aggagatcga gttcgggacc
     2461 tgggtgcagc gcatgcgctc gagcgaagcc acgacggcgc ggctcaccga gatgctgcgc
     2521 gatgagcctt tgcgctcttt cctgaagccg cgcgagaccg cgtcagggcc ggatttcacg
     2581 ctgacggagg gaattatcct tggccgcaag ccgcgctaga ggcctcgact ggcagggcgc
     2641 ccaggcgccg tctctcgccg agctcgaggc gattgccgac caggcgttca agcgcctgcc
     2701 ccgccgcttc cgcgatcttt gcaaggacgt cgtggtcagg gtcgaggatt tccccaccga
     2761 cgaggtgctc gacgcgctcg acgccgagtc gcccttcgat ctgctcggcc tctatcatgg
     2821 cgtcgacatg gcgcggcaaa gcgtcatgga ccctgccgcc atgcctgaga tggtgttcct
     2881 ctatcgccgg ccgatcctcg actactgggc cgagcacgaa gagacgctag ggcacatcgt
     2941 cacccatgtg ctggtgcatg aaatcggtca tcatttcggc ctttccgacg ccgacatgca
     3001 tcgcatcgag gaagaagccg cggcttgacc caggtcggag cgcttaccgg agtcgtcata
     3061 ccggcgaaag ccggtatcca gtttcttttt tgcgcttcgc gcgctggatt ccggcttgcg
     3121 ccggaatgac gaaagtcacc agtccccgtt cgcttcgccg gtcacgcggg cggcgatggc
     3181 cgcaaccaag agctcgtagt cggcgcaagg cccgccgcag cggtcggcga tctccatcag
     3241 ctgctctttc gccttcgcca cgtcgtcctt cagcagatag gcctcgccga gatattgtcg
     3301 cgccttagtg tagttgggat cgattgccag ggcctggtga taatagtcga tgcccttgtc
     3361 gacatcgccg agcttgcgcg tcgagtagcc aatcaggttc aggacctgcg gatcgttccg
     3421 gtccctgatg cggaagagga gcggcaacgc gtcgacgtaa cgggcctcaa gcacaagatc
     3481 gccggccgcg agatagagat cctcatcagt caatgaagcg gagttgccgt cgaggcaagc
     3541 atcggcgccg ccgctccaga cttgcccggt tccgcaactc gtcttgacgc agtcgcgctt
     3601 tctctcgctg tagcgctcac ctttgggaca ggcctttgtc ctgccggtct ttgtgtcgtc
     3661 cttgttgaat tgacgcatct cctcgactgc gtgagcccgc ggcacggcga ggacaaaccc
     3721 gaccaggact gccaacgccg caaagagggc cgttgaacct cttgctgcag tcatgttcgc
     3781 tctaccagtc gacggtctgc tcgccggtga cgtgcgcggc gatcgccttg accagaagct
     3841 catagtcgtc gcaaggcccg ccgcagcggt cggcgatctc catgagctgc gctttcgccc
     3901 cctccacgtc acccttttgc agataagcct cgccgagata ttcgcgcacc ttggtgtagc
     3961 cgggatcgat cgccagcgcc tggtggtaat agccgatgcc cttgtcgata tcgccgagct
     4021 tgcgcgtgga gtagccgatg gagccgagca ccctcgcctg ctcccggttc ttgatgcgga
     4081 agaagagctt cagcgcctcg gcatagtgcg cctgctggcc gagctcgcgc ccggcaatgt
     4141 agaggtcgtc atcggtcaga gacgccgagt tgccgtcgac gcaagatcca acgccgctgc
     4201 tccagatttg cccggttccg caactcgtgc tgacgcagcc atgcttcttc tcgctatatc
     4261 tttttccgct gggacagctt ttgctggctt tcgtgctgct gctatcgtcc gtatcctcga
     4321 cggcaaaggc ctgcggcatg gccaaagcaa gcaagagcaa aattgccccg gcgcctaacg
     4381 ccgtcctaaa tccaatcgac ttggccatga ctccgcatcc ttcaatctgt agctgttggc
     4441 tacaaccgct agcatgccgc gccgcctacg agcatgacgt cgcggcggca atcacgcgat
     4501 tttgaaacgg aaagcgacgc ccataggccg ccgttttgcc gttgacggag gctccgcccc
     4561 gcctccagac ttgcccgggg ctcattcaaa tcactgctca agggcagcaa ggaagcaaag
     4621 catggggtcg gctgcccgct taaggggcat tattgtgttg tttctggcgc tcgccgtggc
     4681 aggccccgag cctcaggctg tcgccatcga gaaggacaag ctcgtgcccg cagggcgtct
     4741 ggtgatttcc gggcggcggc tctcttgcgg cagcacccag acgctgctca gggatttcga
     4801 gggcttcgcc gtctcctcca cggtaatcat gctcaacatg caggcgctcg accctctgcc
     4861 gcggcaggtg caatggctga tctactatca cgaatgcggg catatcctgt acggcccgag
     4921 cgagacctcg gccgattgct acgctgtgcg gcgggcgcgg cgcgagggct ggctgaacga
     4981 ccaagcgctt aacgacatct gcgcggtgtt caacatcgcc ggccacggcc cggtgcatcc
     5041 ggacccgcaa gcgcgctgcg atcagcttcg ccaatgcttc ggcaaggccg gcgtgacggc
     5101 ccgtgagggc gacaaatagc gcaggattcg gggcctcgga gcgagattgc gcaaagcccg
     5161 ttcgccccgt aagctgcgcc ttatgaggga tgcgccaatg cgaattgggg gggcgctttt
     5221 cggcgtcttg gtctgcggaa gccttgcctt ccagccgtcg ccggcgcgcg cccaggaaca
     5281 caccttcctg gacggcccaa gcggcccgac catcgacgac tatctgaagc aggccggacc
     5341 agacgtgaag ctcgtcccgc aaggctccct caagatcgac ggcaggacgg tgaattgcgg
     5401 caagcggccg acggtgctga actcgaattt cgacagttgg ggcggcgcct tcccgggctt
     5461 cgtcattcta aacaccaaga agatcaccgg gctctcaacc gcggtgaagc tttacgtcta
     5521 ttcgcacgaa tgcggccacc agttcgaagg gccggacgag accaaggccg atttgttcgc
     5581 catcaggcgc ggcgtgaaat ggggctggct cgacgccgtg ggcatggagg agatctgcac
     5641 cttcatctcg acgctgaagg gcgatgcggt gcatccgcca gggccccagc gctgcgagac
     5701 catgcgcaat tattacaagc agctgatcga gagcggcacc acgcagtcgg cgagcacacc
     5761 tgcggctgag cccgtctcca agcagaaggc gcaagcgctg gcgccgcagc aataggcgcc
     5821 cgttttctcg ccggattctt gtgtcatggc cggacttgtc ccggccgccc acgtctctat
     5881 attgccgttc atggaaaaat tcaccacgct taccggcatc gcggcgccct tgccgatgat
     5941 caatgtcgac accgacatga tcatcccgaa gcaatatctg aagacgatca agcgcaccgg
     6001 gcttggcaca ggcctgttcg ccgagttgcg ctatgacgag gagggcaagc cccatcccca
     6061 cttcgtgctg cacaagccgc cttacactga agcaaagatt ctgatcaccg gcgagaattt
     6121 cggctgcggc tcatcgcgcg agcacgcgcc ctgggcgctg ctcgatttcg gcttccgctg
     6181 cgtcatcgcg ccaagcttcg ccgacatctt ctacaacaac tgcttcaaga acggcatcct
     6241 gccgatcaag ctaccgcaga gcgagatcga caagttgctc gacgatgcct cgcgcggggc
     6301 gaatgctagc atcaccgtcg atcttgagaa ccaggagatc agaggccccg acggcggcgt
     6361 gattcacttc gacatcgatc cgtttctcaa acgctgccta atggaaggcc tcgacgatat
     6421 cggcctcacg ctggagaagg ccgagcacat cgatagcttc gagaccgagt ctcgcacccg
     6481 ccgcccctgg gcttgagccg tagggtgggc acatgcgcga aagcgccgtg cccaccggtg
     6541 ggctccgcgt gggcaacggc agaccgttgc ccaccctact caaactactc gaacgagatc
     6601 ggctgtgagc tttgctcgac caacgtcttg ccgtcgcggg tgatcgtcag cgtcgcgtga
     6661 tagctgcctt ccggccagcc gcccgccggc acgccgcgct ttcctgcctg caacaggagc
     6721 ttcgcctggt cctccgcaag cgtctgcgcg ttgtgcagca acgatctgtc ctcgttgacg
     6781 agcgcgatct cgacgatgtc gccctttttc aggttggccg cgagcacata ggctatggcc
     6841 ggcgtgttga aatgcggcgc cttcagcgct tcgcctttct cgatctcgtc ttgggtggcc
     6901 tcgtggtcgg tgagccccaa ggctagcacc ttggcatcct cggccgctgt cggtgccgcc
     6961 atcccgcaaa tcaaggctaa agcaaagagc gcccttgtca gcatttccgt ctcccattag
     7021 gcaaaggcca cgctctaaca caggaagcgc gcaaagcatg tcatacaaac ttctgctcct
     7081 ccccggcgac ggcatcggcc ccgaagtgat ggccgaggtc tcccgcgtca tcggctgggt
     7141 caatgcgcgc ggggccgcca agatcgagac cgagaccgac cttgtcggcg gctgcgccta
     7201 cgatgcgcat ggggaggcga tctcggacgc gaccatgcga cgcgcatctg ccgccgacgc
     7261 ggtgatgctc ggcgccgtcg gcggccccaa atgggacggc gtcccttacg accagcgccc
     7321 ggaggccggc cttctgcgcc tgcgcaagga cctcgagctc ttcgccaatc ttcgtcctgc
     7381 catctgctac ccggcgctcg ccgatgcctc ctcgctcaag cgcgaccgcg tcgacggcct
     7441 cgacatcatg attgtgcgcg agctgaccgg cggcgtctat ttcggcgagc cgaaggagat
     7501 gaaggatctg gggaacggcc agaagcgcgc cgtcgacacc caagtctacg agacctacga
     7561 gatcgagcgc attgcggccg tcgccttcga gctcgcgcgg cttcgccgca accaggtgca
     7621 ttcggccgag aagcgcaacg tgatgaagac cggcgtgttg tggaacgagg tggtaacccg
     7681 cctgcacaag gagcgctact cggacgtcac gctccaccac atcctcgccg acaattgcgc
     7741 catgcagctc gtgcgcgcgc cgaagcagtt cgacgtgatc gtcaccgaca atctgtttgg
     7801 cgacatgctg tcagacgagg cgtcgatgct caccggctcg ctcggcatgc tgccgtcggc
     7861 ttcgctcggc gccgccgacg agcgcggcca ccgccgcgcg ctctacgagc cggtgcacgg
     7921 ctcggcgcct gacattgccg ggcgcggcat cgccaacccc atcgccacca tcgcgagctt
     7981 cgccatgtgc ttgcgctatt ccttcaatct gggggagacg gcggaccgaa tacaaaaggc
     8041 catcgctttc gtgctcgacg agggttaccg caccgccgat atcgcccagg aaaattgccg
     8101 caccatcggc accgcggaga tgggcacggc catcatcgcc gccctcgacc gcatggctgc
     8161 gtgaccggga tggcatcgac cgacgcgcaa ggaaaatccc tccctgcgag gggagggtgg
     8221 cgagacgcga tttgatcgcg gctcgccggg tggggcggcc tccctgcgct ggacagtccc
     8281 aacgggcccg accatactcc gccggccttt gagctgttgg gcgcgaaaga gggacttatg
     8341 gcgcttctgc gaaccgcttt tgttgccgcc agcatcgcgc ttgccatgag cttgccgccc
     8401 ctcgccgagg agccaggcgc ctcgacaagc ccttcggagg cagcaccgcc ggcaggcgat
     8461 tcaggcaagg atgacctgac cgccgaggag cgggcagaga agcaggcgcg caaggcctgc
     8521 aaggtcaaga tctgcgacat cctcgccacc aagaatccgc aaggcgacga cgtcgcttgc
     8581 gagatcgtca agacttggcg cgagcaggac atcaccaaga tgcttggcgg caggttcaac
     8641 tggacctggg gcaaggcggt ctgccaatcg aagctcgagg tcaagcgcga ggctctggtg
     8701 aaggcgatga ccgaggcgaa ttacgatgtg gttttgcccg agcagaaagt gcgctgcacg
     8761 ctcgcccaaa agtctgaagg cgagccctat gccgtcgaca tctcgatcgc gccaaaggtc
     8821 gccttcgaga gcggcaaagc ggtctcggcg aagctcaatt ggggcgaggc taaagcgccg
     8881 atgctcgcct atgccttgat ctatgccggc accgggctcg acaattcagc caacatccta
     8941 ggcccggaag ttacacgcat ggtgaacgag ttcaccacca agaaatgcgc caagctcaag
     9001 gacgatacgc cgagaagtac gggcaattaa ccgaggaaac cgagcgatgt actacagcgc
     9061 accttatttc atcaccatcg gccgcgtgct catggcgatc atgttcatcc agggcggcat
     9121 cgaaaagatg ttcggctatg ccggagcgca aggatatatg gaagcgcatg gcgtgccggg
     9181 cgtgctgttg ccgctcgtca tcctgaccga ggcaggaggc gggttgtgcg tgctgctcgg
     9241 cctgttcacc cgctggtggg cgatcgctct tgccggcttc tgcgtgctcg cggcgctgtt
     9301 cttccattgg catcccgaag accagatgca gatgatcaat ttcatgaaga acatcaccat
     9361 cgccggcggc tttctggtgc tggccggcgc cggccccggc gctctcgccc tcgacaacag
     9421 gctaacgtaa acgccggtcg aatacacaag atctacgagg agaagggaga accgatgacc
     9481 gatgccttca ttgagcaact gcgcccgctt ggctttgaga aatacgcgag tcgtctgacc
     9541 cgcatagtcg gcaacgccac gcaggttgtc gtcggagggg accaagtgcg gagaggtctg
     9601 gcacccgacg agaccggcgc ggttatcgtt ggggatatgg acagcgccgc agcgcggctc
     9661 tcggatgacg gcactttcgt aatcgatcga ctgcgagaac ttggcgaggc cggcctcgag
     9721 gcgat
//
LOCUS       DATNAE010000130         2213 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3435319, whole genome shotgun sequence.
ACCESSION   DATNAE010000130 DATNAE010000000
VERSION     DATNAE010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3435319"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..1662)
                     /locus_tag="VN518_02830"
     CDS             complement(118..1662)
                     /locus_tag="VN518_02830"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXE03538.1"
                     /translation="MSGSASAGAERDALANHKRRAYRSVQEAREKLTYKSGGKPEFEY
                     ELLLMFVRNELSAAVTTPLLAIIVAMGAMFWAPPRELLIWLGTVFVTKGILISLCRQF
                     IRAPRDDSKTPMWRAKITAAEFLYGVSWAAVAFISPHTQSEAAHTFIFASMLVVISMR
                     MLFASTVMPIVYAGTLPMTVAIVIRFSLLDNPFYWAMAAMAVGVHIYFIWLMSGLNST
                     VVAMLEFRAEKDALIAELEQSKSISDEARARAEEANLAKSRFLATMSHELRTPLNAIL
                     GFSEIMRAEILGPHANPTYKEYAGDIHQSGQHLLNLINEILDISRIEAGRYELHEAPI
                     DLAELVEDCHRLMRLRAESKGLKIVEAFEENLPQLWADERAIRQICLNLLSNAIKFTP
                     SGGTIALKVGRTGTGGQFLSVKDTGPGIPENEIPRVLKSFGQGSLAHQTAEGGTGLGL
                     PITKGLTELHDGAFELKSKLRYGTEVIITFPRKRVMEALPRVPEPAELRTERPKRPTW
                     RASAKR"
     gene            1841..>2213
                     /locus_tag="VN518_02835"
     CDS             1841..>2213
                     /locus_tag="VN518_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXE03539.1"
                     /translation="MRLTIIGCGDAFGAGGRLQTSFQVRSSGGNFLIDCGTTSLIGMR
                     RLGLQPNEIDTVFVTHLHGDHFGGLPWLLVDAIYVSNRTRPLVVTGPRGIEARFLTAA
                     EALYPDITKAKRSFELSFVEYA"
BASE COUNT          372 a          691 c          743 g          407 t
ORIGIN      
        1 gccgagccgg cggcaacgcc cgaaggcccg ccaccgcctc cggccggcaa gggcaacgtt
       61 gtcgatggtc cttggggccg cgcgatcgaa gcctgatcga ccggaaccct gtagcgtcta
      121 ccgcttggcg ctggcgcgcc aagtggggcg tttcggccgc tcggtgcgca gctcggcggg
      181 ttcgggcacg cggggaagcg cctccatcac gcgcttgcgc gggaaggtga tgatcacctc
      241 ggtgccgtaa cggagcttgc tcttcagctc gaaggcgccg tcatgcagct cggtcaggcc
      301 cttggtgatg ggcaggccga gtccggtgcc gccttcggcc gtctgatgcg cgagcgagcc
      361 ctggccgaag ctcttcagca cgcgcgggat ctcgttctcg ggaatgcccg ggccggtgtc
      421 cttcacgctg aggaactgac cgccggtgcc ggtgcgcccg accttcagcg cgatggtgcc
      481 gcccgaaggg gtgaacttga tggcgttgga gagcaggttg aggcagatct ggcggatggc
      541 gcgctcgtcg gcccagagct gcggcaggtt ttcctcgaac gcctcgacga tcttcagccc
      601 cttgctctcc gccctgagcc gcatcagccg gtgacaatcc tcgaccagtt cggcgagatc
      661 gatcggcgcc tcgtgcaatt cgtagcggcc ggcctcgatg cggctgatgt cgagaatttc
      721 attgatgaga ttgagcagat gctggccgct ctgatggatg tcgccggcat attccttgta
      781 ggtcgggttg gcgtgcggcc ccaggatctc cgcgcgcatg atctcggaaa agccgaggat
      841 ggcgttgagc ggggtacgca gctcgtggct catggtggcg aggaagcgcg acttggcgag
      901 gttggcctcc tcggcgcgcg cccgcgcctc gtcggagatc gatttcgact gctcgagctc
      961 ggcgatcagc gcgtctttct cggcgcggaa ctcgagcatg gccacaacgg tcgagttgag
     1021 cccgctcatc agccagatga aatagatgtg cacgccgacc gccatggcgg ccatcgccca
     1081 gtagaacgga ttgtcgagca gggagaagcg gatgacgatg gcgaccgtca tcggcaaggt
     1141 gccggcatag acgatgggca tcaccgtgga tgcgaacagc atgcgcatcg agatgacgac
     1201 cagcattgag gcgaagatga aggtgtgcgc cgcctcgctt tgcgtgtgcg ggctgatgaa
     1261 ggcgacggcc gcccagctga cgccgtagag aaactcggcg gcggtgatct tggcgcgcca
     1321 catcggcgtc ttgctgtcgt cgcgcggcgc ccggatgaat tgccggcaca ggctgatgag
     1381 gatgcccttg gtgacgaaca ccgtgccgag ccagatcaga agctcgcgcg gcggcgccca
     1441 gaacatggcg cccatggcga cgatgatggc gagcagcggc gtggtgacgg cggcggacag
     1501 ctcgttccgc acgaacatca acagcagctc gtactcgaat tccggcttgc cgcccgattt
     1561 ataggtgagc ttctcgcgcg cctcttgcac gctgcggtag gcgcgacgct tgtgattggc
     1621 cagggcgtct cgttccgccc ccgctgacgc gctccctgac atgctaaagc tctacccgca
     1681 aagcgacgtg actgacaaaa agacgagggc tgccagcggc ataagccttg ggcaggctgt
     1741 cctcgacccc ctgatggacc gcctggaact atctcgctta atccctaacg gcgcgtttac
     1801 tctcgctccg tgctgagacg aacactaaga tgccggcacg atgcgactga ccatcatcgg
     1861 ctgcggcgat gccttcggtg cgggcgggcg cctacagacc tcattccagg tgcgctccag
     1921 cggcgggaat ttcctgatcg attgcggcac cacctcgctg atcggtatgc gccggctcgg
     1981 gttgcaaccg aacgagatcg acaccgtgtt cgtcacccat ttgcatggcg atcatttcgg
     2041 cggcctgcct tggcttttgg tcgatgcgat ctatgtcagc aaccgcacgc ggccgctcgt
     2101 ggtcacgggg ccgcgcggca tcgaggcgcg tttcctgacc gccgcggagg cgctctatcc
     2161 cgacatcacc aaggcaaagc gcagcttcga gctgtccttc gtcgaatatg cgg
//
LOCUS       DATNAE010000131         2415 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2232306, whole genome shotgun sequence.
ACCESSION   DATNAE010000131 DATNAE010000000
VERSION     DATNAE010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2232306"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            290..1444
                     /gene="recA"
                     /locus_tag="VN518_02840"
     CDS             290..1444
                     /gene="recA"
                     /locus_tag="VN518_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HXE03540.1"
                     /translation="MVNVALRLVEGAEVDKEKALEAAISQIERNCGKGSIMRLGQNEK
                     AVEVEAISSGSLGLDIALGIGGLPRGRVVEIYGPESSGKTTLALHVVAEAQKRGGICG
                     FIDAEHALDTVYARKLGVDLEQLLISQPDTGEQALEITDTLVRSGAIDVLVVDSVAAL
                     TPKAELEGEMGDMQMGSQARLMSQALRKLTGSISKSRCMVIFINQIRMKIGVMFGSPE
                     TTSGGNALKFYASVRLDIRRIGAIKEREEVVGNQTRVKVVKNKVAPPFKQIEFDIMYG
                     EGISKTGELVDLGVKAGIVEKSGAWFSFDSERIGQGRENAKAYLKEHPDMAAKIERAI
                     RQSAGLIAGRILEGAGEPDAADGGEAGFAEGESGVTPLPGGAARRGRRGV"
     gene            1633..>2415
                     /locus_tag="VN518_02845"
     CDS             1633..>2415
                     /locus_tag="VN518_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HXE03541.1"
                     /translation="MTSAKTKGAKETSRKPQASARADDAPGGGVSHTHLASSSTNDIR
                     SGFLDFFARNDHAVVPSAPLVPRHDPTLMFTNAGMVPFKNVFTGAEKPKSLRAASAQK
                     CVRAGGKHNDLDNVGYTARHHTFFEMLGNFSFGDYFKERAILLAWELLTKDLALDKSR
                     LLITVFADDDEAAALWRKIAGLPDERIIRIAGSDNFWSMGDTGPCGPCSEIFYDHGPS
                     IGGPPGSTDADGDRFVEIWNLVFMQYEQLPGGERVSLPKPSID"
BASE COUNT          449 a          794 c          779 g          393 t
ORIGIN      
        1 atgatcttgt gcggcaggat ggcgctttcg cagccctggc gaaggctcaa ttcatcaacc
       61 cgcctgactt tgcgccgtcc tgaggcggcc tctgtgtttt ccgcgctgtg aggaacataa
      121 aaagaacatt gcaggctgag aacaaaacgt gtacagtttt ctgacttcgc atctcgcatc
      181 tctcctcgcg acccaagccg accaaggctg catggcactc ccggggaggg tgcggcggag
      241 ccacccattt gcgactcaga gcgtgcttgt gaaataaggg agagcctcca tggtgaatgt
      301 cgcgctgcgc ctcgtcgaag gagctgaagt ggataaagag aaggcgcttg aagcggcgat
      361 ctcgcagatc gagcgcaatt gcggcaaggg ctcgatcatg cgcctcggcc agaacgagaa
      421 ggcggtcgag gtcgaggcga tctcctccgg ctcgctcggc ttggacatcg cactcggcat
      481 cggcgggctg ccgcgcggcc gcgtcgtcga gatttacggg ccggaatcgt cgggcaagac
      541 cacgctcgcg ctgcatgtcg tggcggaagc gcagaagcgc ggcggcattt gcggcttcat
      601 cgatgccgag cacgcgctcg ataccgtcta tgcgcgcaag ctcggcgtcg atctcgagca
      661 gctcctgatc tcccagcccg acaccggcga gcaggcgctc gagatcaccg acacgctggt
      721 gcgctccggc gccatcgacg tgctggtggt cgattcggtg gcggcgctca cgcccaaggc
      781 cgagctcgag ggcgagatgg gcgacatgca gatgggcagc caggcgcggc tgatgagcca
      841 ggccttgcgc aagctcaccg gctcgatctc gaaatcccgc tgcatggtga tcttcatcaa
      901 tcagatccgc atgaagatcg gcgtgatgtt cggcagcccc gagaccacga gcggcggcaa
      961 tgcgctcaaa ttctacgcct cggtcaggct cgacatccgc cgcatcggcg ccatcaagga
     1021 gcgcgaggag gtggtcggca accagacccg ggtcaaggtg gtgaagaaca aggtggcgcc
     1081 gcccttcaag cagatcgagt tcgacatcat gtatggcgag ggcatctcca agaccggcga
     1141 gctggtcgat ctcggggtca aggccggcat cgtcgagaag tccggcgcct ggttctcctt
     1201 cgacagcgag cgcatcggcc aaggccgcga gaacgccaag gcctatctca aggagcatcc
     1261 cgacatggcc gcgaagatcg agcgggccat tcgccaaagc gcgggcctga tcgccggccg
     1321 gatcctcgaa ggcgcgggcg agcccgatgc cgcggacggc ggcgaggccg ggttcgccga
     1381 gggcgagagc ggcgtgacgc cgctacccgg cggagcggcc cggcgcggcc gccgcggcgt
     1441 ttaaccagag cgcttagcca gccgcgcggc ctgctggaca ggccgcgtgg cccaccttat
     1501 gtctggcgcg acacctttag ccctgtccct gaactcgggg tgtgggctaa cggcgcttaa
     1561 accatggccg aatcggctag ggtccataag cgccggctcc gctcgactct aaggcctccc
     1621 gctaaagccg ccatgacctc cgccaagacc aagggtgcga aagagacaag ccgcaagccg
     1681 caagcctcgg cccgcgccga tgacgcgccg ggcggcggcg tgagccacac ccatcttgcc
     1741 agctcgagca ccaacgacat ccgctcgggc tttctcgatt tcttcgccag gaacgaccac
     1801 gcggtggtgc cctcggcgcc gctcgtgccg cggcacgatc caaccttgat gttcaccaat
     1861 gccggcatgg tgccgttcaa gaacgtgttc accggcgccg agaagccgaa atccttgcgc
     1921 gcggcctcgg cgcagaaatg cgtgcgcgcc ggcggcaagc acaacgacct cgacaatgtc
     1981 ggctataccg cccgccacca caccttcttc gagatgctcg gcaatttctc cttcggcgac
     2041 tatttcaagg agcgcgccat actgctcgcc tgggagctgc tcaccaagga cctcgcgctc
     2101 gacaagagcc gcctgctcat caccgtgttc gccgacgacg atgaggcggc ggcgctgtgg
     2161 cgcaagatcg ccgggcttcc cgacgagagg atcattcgca tcgcgggctc cgacaatttc
     2221 tggtcgatgg gcgacaccgg cccgtgcggc ccgtgctcgg agatcttcta cgaccatggg
     2281 ccgtcgatcg gcggcccgcc cggcagcacc gacgccgacg gcgaccgctt cgtcgagatc
     2341 tggaatctcg tcttcatgca atacgagcag cttccgggtg gcgagcgcgt cagcctgccc
     2401 aagccctcga tcgac
//
LOCUS       DATNAE010000132         6854 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_4341858, whole genome shotgun sequence.
ACCESSION   DATNAE010000132 DATNAE010000000
VERSION     DATNAE010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6854
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4341858"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VN518_02850"
     CDS             complement(<1..189)
                     /locus_tag="VN518_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HXE03542.1"
                     /translation="MPFRSPQLSWALSREPVWPYRGPLSTSSIEYFRHGAFRKDRPAM
                     RIEIGNDGWSWPKGAPVTT"
     gene            361..1746
                     /locus_tag="VN518_02855"
     CDS             361..1746
                     /locus_tag="VN518_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXE03543.1"
                     /translation="MTAAEGRRREGWGERLARAFAVFLDPRVLIILLLGFSSGLPLAL
                     SASTLLLWMKDVGVDLKTIGLFALVGVPYTIKFLWAPIVDAFQVPILSKALGHRRGWL
                     VFTQLLLIASILFLGFQDPVRAPLMVALAALLVATASATQDIVIDAFRVEYLPTDAQA
                     AGMAYFVAAYRIGMLVSTAGSVAFVAYLEYLGIDRELVWTYGYAAMAALVLVGMAAAF
                     MAKEPRAKEREAEAEALGERTGNPLNRFFRAAYDAFADFLSKKDALLILLFVILYKLT
                     DTFAGVMTGPFVLDIGFDKASYAAIIKGVGLIAALAGGVAGGVLARAMPLSRALLVAG
                     ILQAVANFTFSWQAWIGVNHAALALAISTENFTSAVGTVVFIAYLSALCTSPAHTATQ
                     FALLTAVASFGRTTLASITGFVAAATGWVVFFALCAAAAIPGLLILWILIKRGNFEDI
                     ERKERAEAAEF"
     gene            complement(1803..2162)
                     /locus_tag="VN518_02860"
     CDS             complement(1803..2162)
                     /locus_tag="VN518_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03544.1"
                     /translation="MNKLMTILVAALALALVAFTPDLASAGHGHGGGRGWHGGGHWHG
                     GGGHWRGGHAWRGGRGWHGGWHGHRGWYGYRGWRGYGWGGWWGPGINIYVGGYGRRCW
                     SPAYGYYPCHYRYGWGY"
     gene            complement(2355..2960)
                     /gene="recR"
                     /locus_tag="VN518_02865"
     CDS             complement(2355..2960)
                     /gene="recR"
                     /locus_tag="VN518_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HXE03545.1"
                     /translation="MARAAASAEIERLIQLLARLPGLGPRSARRAALHLVKKKEQLLA
                     PLAAALTEAHDRIVTCETCGNIDTVSPCTLCRDQSRDRTLICVVEEVGDLWALERAHV
                     LNGLYHVLGGTLSPLDGVGPEDLSIAKLIERAGAPEVKEVLLALNATVEGQSTAHYLT
                     DQLAGCNVTISRLAHGVPIGGELDYLDEGTLAAAVKARKTM"
     gene            complement(2967..3287)
                     /locus_tag="VN518_02870"
     CDS             complement(2967..3287)
                     /locus_tag="VN518_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HXE03546.1"
                     /translation="MKNFAGMMKQAQELQTRMAEMQAEMERARVEGQSGGGLVLLTLN
                     GKGELAGVKIDASLAKPEEVEILEDLIVAAHADAKAKVERLLQEKMQSLTGGLPLPPG
                     LKLF"
     gene            complement(3284..5116)
                     /locus_tag="VN518_02875"
     CDS             complement(3284..5116)
                     /locus_tag="VN518_02875"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF006585.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HXE03547.1"
                     /translation="MAEDKDQEESAPKRSGKKPAKAGGTPAPASGGQSHAALARKYRP
                     KLFSELIGQDAMVQTLRNAFASGRIAQAYMLTGVRGVGKTTTARLIARALNYVPRGGE
                     GAPTLDMTEEGEHCRAILESRHLDVVEMDAASHTGIDDVRDLIDSAHYKPNTARYKVY
                     IIDEVHMLSKQAFNGLLKTLEEPPEHVKFIFATTEARKVPVTVLSRCQRFDLKRIEVE
                     QLAQHLAGIVAQENAAADPAALMLIARAAEGSVRDALSILDRAIAFGSGKVETEAVRG
                     LLGLADRGRIFDLLETVLAGDAGGALAKFAGLTNDGAEPAQAITDLADAVHAVTLVKA
                     ASSSDPAASEAERASAADLAKRLSMPVLARAWQMLLKGHDEVKSSARPHAAADMVLVR
                     LAYAADLPTPGDLMRRLGDRSGEAESAPRAMPNKQAPREPAPAMRQDAPPPATEPSPV
                     DETEDPAPPPQPSLASPKSFEEVVALADEKRDLKLKHALSEQVRLVRFRPGHLELNPL
                     GTAPKELGQDLMRKLKAWTGRVWIVALSGEEGAALLGVQRRERKAREIERIRDHPDVK
                     QVLQHFPGARIAAVRATASEEPPAPPAEIDDGAEAEFKEDGTDP"
     gene            complement(5210..5306)
                     /gene="ffs"
                     /locus_tag="VN518_02880"
     ncRNA           complement(5210..5306)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VN518_02880"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5379..5864)
                     /locus_tag="VN518_02885"
     CDS             complement(5379..5864)
                     /locus_tag="VN518_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6790 family protein"
                     /protein_id="HXE03548.1"
                     /translation="MIALLIAVLMVVLPLLHLAFSKMPRTPERVVHILLLYALVFGVG
                     IIGLPMGFIPHVFFPDQTAESIGWPTGSPFQFEVGLHDGAWGVLGFLCIWIGGLFWLA
                     TGLGWSLFMLGAAYGHIRETVIKGDYAPYNFFTMFSDGFIGIWILVLLYLYWRQGGFR
                     K"
     gene            complement(5883..6740)
                     /locus_tag="VN518_02890"
     CDS             complement(5883..6740)
                     /locus_tag="VN518_02890"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HXE03549.1"
                     /translation="MSSRSQKIAYQGEPGANSHLACRDAYPKMEPLACPTFEDALAAV
                     KGGEARLAMIPIENSVAGRVADIHHLLPDSGLFIVAEHFERIRHQLLTLPGVKLSELK
                     TVHSHTMALGQCRKAIRALKLKPVTEADTAGAARLLRDTQDRSRAVIASALAAEVYGL
                     KIAKSGIEDAKHNTTRFVVLAAKPDHAKAKDGPVITTFVFRVRNVPAALYKALGGFAT
                     NGVNMTKLESYQLEGSFNATMFYADIEGHPDERPVKLAMEELAFFTSEVRVLGTYRAS
                     PFRAELKAG"
     gene            complement(6737..>6854)
                     /locus_tag="VN518_02895"
     CDS             complement(6737..>6854)
                     /locus_tag="VN518_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015338295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HXE03550.1"
                     /translation="LRALEAGMRIHVALVDTIPLGVDTPADLARARQILSKS"
BASE COUNT         1124 a         2249 c         2251 g         1230 t
ORIGIN      
        1 ggttgtgacc ggcgcgccct tcggccaaga ccagccgtca ttgccgatct cgatgcgcat
       61 cgcgggacgg tccttgcgga aggcgccgtg gcggaaatac tcgatgctcg aggtcgaaag
      121 cggtccgcga tagggccata ccggctcacg cgaaagcgcc cagctcagct gtggagagcg
      181 gaaaggcaaa ggcgagaccg gcggcgcttg cgctccccag aatgagcccg cgacggctga
      241 tggtcgacac cctgttgtcc cccctcggaa atatcccgcc cattatgcct cgcatccgca
      301 caggcggcag caaaaaggac ggggcatgac ggcgcctgaa tcggctgcta tagcctcccc
      361 atgacggctg ccgagggacg ccgacgggag ggttggggcg agcgtttggc gcgcgctttc
      421 gccgtttttc tcgacccccg cgtgctcatc atcctgctcc tcggattctc gtccgggctg
      481 ccgctagcgc tctcagcctc gacgctgctc ctgtggatga aggatgtcgg cgtcgacctt
      541 aagaccatcg ggctgttcgc gctggtcggc gtcccttaca ccataaaatt cctgtgggcg
      601 ccgatcgtcg atgccttcca ggtgccgatc ctgagcaagg cactcggcca tcgccgcggc
      661 tggctcgttt tcacccagct tctgctcatc gcgtcgatcc tgttcctcgg cttccaggat
      721 ccggtgcggg cgccgctgat ggtcgcgctc gccgcactgc tcgtggccac agcctcggca
      781 acgcaagaca tcgtcatcga cgccttccgc gtcgaatatc tgccgaccga tgcgcaagcc
      841 gcaggcatgg cctattttgt cgccgcctat cgcatcggca tgctcgtctc gacggcgggc
      901 tccgtcgcct tcgtcgccta tctcgaatat ctcggcatcg accgggaact ggtctggacc
      961 tacggctatg ccgcgatggc ggcgctcgtg ctcgtcggca tggcggcggc gttcatggcc
     1021 aaggagccgc gcgccaaaga gcgcgaggcc gaggccgaag cgctcggcga gcggaccggc
     1081 aatccgctca atcgcttctt ccgcgcggcc tacgacgcct tcgccgactt tctctccaag
     1141 aaggacgcgc tgctgatcct cttgttcgtc attctctaca agctcaccga caccttcgcc
     1201 ggcgtgatga ccggaccgtt cgtgctcgat atcggcttcg acaaggcaag ctacgccgca
     1261 atcatcaaag gcgtcggcct gatcgcggcg ctcgccggcg gcgtcgccgg tggcgtgctc
     1321 gcccgcgcca tgccgctctc gcgcgcgctt ctggtcgcag gcatcctgca ggcggtggca
     1381 aacttcacct tttcctggca ggcgtggatc ggcgtcaatc acgcggcgct cgcgctcgcc
     1441 atctccacgg agaatttcac ctcagccgta ggcactgtgg tgttcatcgc ctatctttcg
     1501 gcgctgtgca cgagcccggc gcataccgcg acgcaatttg ctctcctcac cgcggtcgcc
     1561 tcgttcgggc gcaccacgct cgcctcgatc accggctttg tcgccgccgc caccggctgg
     1621 gtcgtgttct tcgcgctgtg cgccgccgcc gccatcccgg ggctcttgat cctgtggata
     1681 ttgatcaagc gcgggaattt cgaggacatc gagcgcaagg agcgcgccga agctgccgag
     1741 ttctgaggcg cgcgtcgacg gcgcgcctca ggatgacgga ttaaagaatt ggcctctcca
     1801 ccctagtacc cccagccgta gcgatagtgg cagggataat agccgtaggc cggagaccag
     1861 cagcggcggc cgtagccgcc cacatagatg ttgatgccgg ggccccacca gccgccccag
     1921 ccgtaaccgc gccaaccgcg atagccgtac cagccacgat ggccgtgcca gccgccatgc
     1981 caaccccgac cgccgcgcca agcgtggccg ccacgccaat ggccgccgcc gccatgccag
     2041 tggccgccgc cgtgccaacc gcggccgccg ccgtggccgt gtcccgccga ggcaagatca
     2101 ggcgtgaagg cgacgagcgc cagcgcgagc gccgcaacga gaatcgtcat gagcttgttc
     2161 atcttgagcg tcctcttgaa cgaggccgtt cggtctttat cggcgacccg cctttcgatc
     2221 ggcctgaccc atgcccgccg atccgggcga ttcgatttgg ccgattgcgc tcccgattga
     2281 ggaagcggtc aagttggcct gtgggattag cccataatac gcgccggtaa ggttcgcggt
     2341 tccattgagc aggctcacat cgtcttgcgg gctttgaccg ccgcggcgag cgtgccttcg
     2401 tcgagataat cgagctcacc gccgatcggc acgccgtgcg cgaggcgcga aatggtgacg
     2461 ttgcagccgg cgagctggtc ggtgaggtag tgcgcggtgg actgaccctc gaccgtggcg
     2521 ttgagcgcaa gcagcacttc cttcacctca ggcgcgccgg cgcgctcgat cagcttggcg
     2581 atggagagat cctcggggcc gacgccgtcg agaggcgaca gcgtgccgcc aagcacgtga
     2641 taaagcccgt tcaacacatg cgcccgctcg agcgcccaga ggtcgccaac ctcctcgacc
     2701 acgcagatca gcgtccggtc gcggctttgg tcgcggcaga gcgtgcacgg actgacggtg
     2761 tcgatgttgc cgcaagtctc gcaggtgacg atgcggtcat gagcttcggt gagtgccgcg
     2821 gcgagcggag caagaagctg ctccttcttt ttgacgagat gcagcgcggc acggcgcgcc
     2881 gagcgcggac ccaagcccgg caggcgcgcc agcaattgga tgaggcgctc gatctcggcg
     2941 cttgcggcag cccgcgccat gcgacctcag aacagcttca ggccgggagg gagaggcagg
     3001 ccgccggtca ggctctgcat cttctcctgc aacagccgtt cgaccttggc cttggcgtcg
     3061 gcatgggcgg cgacgatgag gtcttccagg atctcgacct cctcgggctt cgccagagag
     3121 gcgtcgatct tcaccccggc caactcgccc ttgccgttca gcgtcagcag cacgagaccg
     3181 ccgccggatt gcccctcgac gcgggcgcgc tccatctccg cctgcatctc ggccatgcgg
     3241 gtctgcagct cctgggcctg cttcatcatg ccggcaaaat tcttcatggg tcggtcccgt
     3301 cctccttgaa ctcagcctcg gccccatcgt cgatctcggc cgggggggcc gggggctcct
     3361 cgctcgcggt cgcccgcacc gcagcgattc gcgcgccggg gaaatgttgc agcacttgct
     3421 tgacgtcggg gtgatcgcga atgcgctcaa tctcgcgcgc tttgcgctcg cggcgctgca
     3481 cgcccaagag tgctgcgccc tcctcgccgc tcaaggctac gatccatacg cggccggtcc
     3541 aggccttgag cttgcgcatc aggtcctggc cgagctcctt cggcgcggtt ccgagcgggt
     3601 tgagctcgag atgccctggg cggaagcgca cgagccgcac ttgctcggac agcgcgtgtt
     3661 tgagcttcaa atcgcgcttc tcatcggcga gcgcgacgac ctcctcgaag ctcttagggc
     3721 tggcgagcga gggctgaggc ggaggggccg ggtcttccgt ttcatcaacc ggcgatggtt
     3781 ccgtcgccgg cggcggcgcg tcctgccgca tggccggagc gggctcgcgc ggagcctgct
     3841 tgttgggcat cgcgcgaggc gcgctctccg cttccccgct cctgtcgccg aggcggcgca
     3901 tcagatcgcc gggcgtcggc agatcggcgg cataggcgag ccgcaccaga accatgtcgg
     3961 cggcggcatg tggcctagcc gaggacttca cctcgtcatg gcctttcaac agcatctgcc
     4021 aggcgcgggc gaggaccggc atcgagaggc gtttggcgag atcggcggcg cttgcccgct
     4081 cggcctcgct cgccgcaggg tctgagctgc tggcggcttt gaccagggtc accgcatgaa
     4141 ccgcgtcggc gagatcggtg atcgcttgcg ccggctcggc gccgtcattg gtgagcccgg
     4201 cgaatttcgc aagcgcgccc ccggcgtcgc cggcgagcac ggtctcgagc aggtcgaaga
     4261 tgcggccgcg atcggcaagc ccgagcagcc cgcgcacggc ctcagtttcg accttgcccg
     4321 agccgaaggc gatggcgcgg tcgaggatcg acagcgcatc gcgcaccgag ccttcggcgg
     4381 cgcgcgcgat cagcatcaag gcggcggggt cggcagcggc attttcctgc gccacgatgc
     4441 cggcgagatg ctgggcaagc tgctcgactt cgatgcgctt caggtcgaag cgctggcagc
     4501 gcgacagcac tgtcaccggc actttgcgcg cttcggtggt ggcgaagatg aacttcacat
     4561 gctcaggcgg ctcttccaaa gtcttgagca ggccgttgaa ggcctgcttc gacagcatgt
     4621 gcacctcgtc gatgatgtag accttgtagc gggcggtgtt cggcttgtag tgggcgctgt
     4681 cgatcaagtc gcggacgtcg tcgatgccgg tatgggaggc cgcgtccatc tcgaccacgt
     4741 cgagatgccg gctctcgagg atggcgcggc aatgctcgcc ctcctcggtc atgtcgaggg
     4801 tcggcgcgcc ttcaccccct cgcggcacat agttcagcgc ccgggcgatc aggcgcgcgg
     4861 tcgtggtctt gcccacgccg cgcaccccgg tgagcatata ggcctgggcg atgcgaccgc
     4921 ttgcaaaggc attgcgcagc gtctgcacca tggcgtcctg tccgatcagc tcggagaaca
     4981 gcttcggccg gtatttgcgc gcgagcgcag cgtggctctg accgccggaa gcgggcgcag
     5041 gcgtgccgcc ggctttcgcc ggcttcttcc cggagcgctt cggcgcgctc tcttcctggt
     5101 ctttgtcttc ggccatggac gatcggactt tcacgcggag gtaggaccct cggccagcag
     5161 caaggcaggc tcaaggcctt tacgcacgag cggctacggg gcgcgagagg tgggaggctg
     5221 gacaaacaac ccgcgtcaat ctcgttaggg ctgcttcctt ccggacctga cccggttggc
     5281 gaggaacgcg cctgccgcca acctcccgga tgagtatatc ggggatagcg gcgcctgtgg
     5341 caacccgctg agaagcgcct ttgctctgga cggggtcctt atttccggaa gccaccctgg
     5401 cgccaataga ggtagagaag cacgaggatc cagatgccga tgaatccgtc cgagaacatc
     5461 gtgaagaagt tgtagggagc gtagtcgcct ttgatcacag tctcgcggat gtgcccatag
     5521 gcggcgccga gcatgaaaag cgaccagccg agcccggtcg cgagccaaaa caggccgccg
     5581 atccaaatac aaagaaagcc tagaacgccc caggcgccgt catgcagacc aacctcgaac
     5641 tggaacgggc ttccagtcgg ccagccgatc gattccgccg tctggtcggg gaagaacaca
     5701 tgagggataa agcccatcgg caagccgatg attccgacac caaacaccag ggcgtagagc
     5761 aagaggatgt ggacgacgcg ctcaggcgtt cgcggcatct tgctgaacgc caagtgcaac
     5821 aacggcagaa cgaccatcaa aactgcgatg agcaaggcga tcattgttcc cccctcaacg
     5881 tctcagcctg ccttgagctc ggcgcggaag ggacttgctc tataggtgcc gagcacccgc
     5941 acctcgctcg tgaagaaggc gagctcctcc atggcgagct tgaccggccg ctcgtccggg
     6001 tgaccctcga tatcggcata gaacatggtg gcgttgaacg agccctcgag ctgataggac
     6061 tcaagttttg tcatgttgac gccgttggtg gcgaagccgc caagcgcctt gtacagcgcg
     6121 gcgggaacgt tgcgcacgcg gaacacgaag gtggtgatga cgggaccgtc cttcgccttg
     6181 gcatggtcgg gctttgcggc aagcacgacg aagcgcgtgg tgttgtgctt cgcgtcctcg
     6241 atgcctgact tggcgatctt cagcccatag acctcggcgg caagcgccga ggcaatcacg
     6301 gcgcggctgc ggtcctgcgt gtcgcgcaaa agcctggcgg cacctgcggt atcggcctcg
     6361 gtcaccggct tcagcttcag cgcgcggatc gccttgcggc attggccgag cgccatggtg
     6421 tggctgtgca cggtcttgag ctcgctgagc ttcacgcccg gcagagtgag caactgatgg
     6481 cggatgcgct cgaaatgctc ggcgacgatg aagaggccgg agtcgggcag aagatgatgg
     6541 atgtctgcga cgcggccagc gaccgaattc tcgatgggga tcatggcgag ccgcgcctcc
     6601 ccgcctttga ctgcggcaag cgcgtcctcg aaagtggggc aggcaagcgg ctccatcttg
     6661 ggataggcgt cgcggcaggc gagatgggaa ttggctccgg gctcgccttg ataggcgatt
     6721 ttttgcgatc gactgctcat gatttgctga gaatttggcg tgcccgggcg aggtcggcgg
     6781 gagtatcgac accgagcggt atggtgtcaa cgagggcgac gtgaatgcgc atgccggctt
     6841 ccagagccct cagc
//
LOCUS       DATNAE010000133         3368 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_124645, whole genome shotgun sequence.
ACCESSION   DATNAE010000133 DATNAE010000000
VERSION     DATNAE010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124645"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="VN518_02900"
     CDS             <1..632
                     /locus_tag="VN518_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXE03551.1"
                     /translation="HFISVAEDTGLIVPLGEWVLRRACTDAAKWPDPLIVAVNLSPAQ
                     FRDGDIAETVAEVLKETGLPPERLELEITENLLINDTEEVLGKLNRLRQLGVAIAMDD
                     FGTGYSSLNYLARFPFSKIKIDRQFIRNMTRDPATRAIVRTIIALGKSLDVTITAEGV
                     ETEEQAAMLREFGCPQVQGFLYGYPGAAETGVKPDSNVTSITAARSSAA"
     gene            complement(646..1170)
                     /locus_tag="VN518_02905"
     CDS             complement(646..1170)
                     /locus_tag="VN518_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03552.1"
                     /translation="MIDLKRITIPVFLIYAGVIHAIGLALLLPMIVTLPGPGSMIAPK
                     GVPVAVELDAEAEPDQTAALPAPAEDRAPLQAQPEPVAPQPQADAKPDIPAVLANATP
                     KAQPAKPVLAAPQPKSQAKPTAAKSGKAVKTTVVRASTKPTVRHSAHRDAKIAPFAGG
                     MSGLFTPGAPSKQR"
     gene            complement(1252..2418)
                     /gene="apbC"
                     /locus_tag="VN518_02910"
     CDS             complement(1252..2418)
                     /gene="apbC"
                     /locus_tag="VN518_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006726628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HXE03553.1"
                     /translation="MTETPSKERVLEALKRVKGPDLSSDIVSQGLVSEIVINKGKVYF
                     AISVDPGRAGELEALRQAAESVVKSLPGVDGVVVTLTADRASGTTRGGNGKGQAPAPQ
                     GPAFTQPGAARDARHRPGGVPGVANIIAVASGKGGVGKSTTAVNFALALKERGLRVGI
                     LDADIYGPSMPRLLGLKGQPQQLDGNKLAPMEAYGLKVMSMGFLVDEETPMIWRGPMV
                     MSALSQMLKDVAWGELDVLVVDMPPGTGDAQLTMAQQVPLAGAVIVSTPQDLALIDAR
                     KGLNMFRKVNVPVLGIVENMSTFICPHCGKASNIFGHGGAKSEATRLGVPFLGEVPLT
                     LQIRETSDAGRPVVASDPTSPAAQAYREIAARAFAELGRKEGGARAAPRIVMEN"
     gene            complement(2464..3279)
                     /locus_tag="VN518_02915"
     CDS             complement(2464..3279)
                     /locus_tag="VN518_02915"
                     /inference="COORDINATES: protein motif:HMM:NF023899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006996 - organelle organization [Evidence
                     IEA];
                     GO_process: GO:0016567 - protein ubiquitination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIDE domain-containing protein"
                     /protein_id="HXE03554.1"
                     /translation="MVAWLVLLGVLLVPSAAFLLWSWRSSRTDMALMRATETTPAAHV
                     AKLPPGSLVELKGRLRCPSPVIGELSNAACAHFVSTVERDYEFFEYDSHRKTSCCKRK
                     TEIVKTTTLFSPFEIEDASGRARVSPELAIIEGIEAVAREDVRRQAAEGESVMQSALG
                     AISNTNRTLCFRYRETHLPVDVDIYVLGVVGEDRCIGAPPQGAKGQRFLISVNSEEAR
                     AAELGSKSRWLLGIGAVCLAGAVVCLGSAAWLARHGLDRPVPPQSVLQNESWW"
BASE COUNT          544 a         1135 c         1096 g          593 t
ORIGIN      
        1 gacatttcat ttccgtggcc gaggatacag gcctcatcgt tccgctcggc gaatgggtgt
       61 tgcggcgcgc ttgcaccgac gcggccaaat ggccggaccc gttgatcgtc gccgtcaacc
      121 tgtcgcccgc gcagttccgc gacggcgaca tcgccgagac tgtggccgag gtgctgaagg
      181 agaccgggct gccgcccgag cggctcgagc tcgagatcac cgagaatctt ctgatcaacg
      241 ataccgagga ggtgctcggc aagctcaacc gcctgcgcca actcggcgtg gccatcgcca
      301 tggacgattt cggcaccggc tattcgagct tgaactatct cgcgcgcttc cccttctcca
      361 agatcaagat cgatcgccaa ttcatccgca acatgacccg cgatccggcg acgcgggcga
      421 tcgtcaggac catcatcgcg ctcggcaagt cgctcgacgt gaccatcacc gccgagggcg
      481 tcgagaccga agagcaggcg gcaatgctgc gcgagttcgg ctgcccgcag gtgcagggct
      541 tcctctacgg ctatcccggg gctgccgaga ccggcgtgaa gccggacagc aacgtgacct
      601 cgatcacggc ggcgcgcagc agcgccgcct agagttttcc ttcttctatc tctgcttgga
      661 tggcgcgccc ggcgtgaaca gcccgctcat gcctccggcg aacggggcaa tcttggcgtc
      721 cctgtgagcc gagtggcgaa cggtaggctt ggtgctcgct cgcacgaccg tcgtcttcac
      781 cgctttgccc gatttggcgg cggtgggctt ggcctggctt ttgggctgag gcgccgcaag
      841 gacgggcttc gccggctgcg ccttgggagt ggcattggcc aggaccgcgg gaatgtcggg
      901 cttggcgtcg gcttgcggct gcggcgcgac cggctcgggc tgggcttgaa gcggggccct
      961 gtcttccgcc ggcgccggca gggcggcggt ctgatccggc tccgcctccg cgtcgagctc
     1021 gacggcgacg ggaacgccct tcggcgcgat catgcttccc gggccgggca gcgtaacgat
     1081 catcggcaac agaagcgcaa ggccgatggc gtggatgacg ccagcataaa tgagaaagac
     1141 cgggatggtg atgcgcttca ggtcgatcac ggcagcgagg agcgaagctt gcgtctactc
     1201 ggcttaccct ggaatcggaa ttagctgagt cgtgaggctc ccggcaagtc tttagttttc
     1261 catcacaatt ctgggtgccg cacgcgcgcc gccctctttt cgtccaagct cggcgaaggc
     1321 gcgcgcggcg atctcgcgat aggcttgcgc ggccggcgag gtgggatcgc tcgccactac
     1381 gggccgcccc gcgtccgagg tctcgcggat ctgaagcgtc agcggcacct cgccgagaaa
     1441 cggcacgccg agccgcgttg cctcgctctt cgccccgcca tggccgaaaa tattcgaggc
     1501 cttgccgcaa tgcgggcaga tgaaggtgct catattctcc acgatgccga gcaccggcac
     1561 gttcaccttg cggaacatgt tgagcccctt gcgcgcgtcg atcagcgcga ggtcctgcgg
     1621 ggtcgaaacg atcaccgcgc cggccagcgg cacttgctgc gccatggtga gctgcgcatc
     1681 gccggtgccg ggcggcatgt cgaccacgag cacgtcgagc tcgccccagg ccacgtcctt
     1741 caacatttgc gacagcgccg acatcaccat cggcccgcgc cagatcatcg gcgtctcctc
     1801 gtcgacgaga aagcccatcg acatcacctt cagcccataa gcctccatcg gcgccagctt
     1861 gttgccgtcg agctgctgcg gctggccctt cagcccgagc agccgcggca tcgatggccc
     1921 gtatatatcc gcatcgagaa tgccaactct gaggcccctc tccttcagcg cgagcgcgaa
     1981 attgaccgcc gtcgtcgact tgccgacacc gcctttgccc gaggcgacgg cgatgatgtt
     2041 ggcgacaccc ggcaccccgc ctggacggtg gcgcgcatcg cgggcggcac ccggctgggt
     2101 gaaggccggc ccttgcgggg ccggcgcttg gccctttccg ttgccgccgc gcgtggtccc
     2161 ggaggcacgg tctgccgtca gcgtcacgac cacgccgtcg acaccgggaa gcgatttcac
     2221 cacgctctcg gccgcctggc gcaacgcttc gagctcgccc gcgcggcccg gatcgacgga
     2281 gatggcgaaa tagaccttgc ccttgttgat gacgatctcg gagacgaggc cttggctgac
     2341 gatgtcggag ctgaggtcgg ggcctttgac ccgcttcagc gcttcgagca cgcgctcttt
     2401 gctgggagtt tcggtcatga tgaggctatg tagtcctgct cggtgccaag accaaccgtc
     2461 ttgttaccac cagctctcgt tttgcaggac gctttgcggc gggaccggcc gatcgagacc
     2521 gtggcgcgcc agccaggccg ccgagccgag gcagaccacg gccccggcca ggcagaccgc
     2581 gccgatgccg agcagccatc ggcttttgct gccgagctcg gccgcgcgcg cttcctcgga
     2641 gttgacgctg atgaggaagc gctgcccctt ggcgccttgc ggcggcgcac cgatgcagcg
     2701 gtcctcgccg accacgccga gcacatagat gtcgacgtcg accggcaagt gcgtttctct
     2761 gtagcggaag caaagcgtgc gattggtgtt gctgatggcg ccgagcgcgc tctgcatcac
     2821 gctctcgccc tccgccgcct gccggcgaac gtcctcgcgg gcgaccgcct cgatgccttc
     2881 aatgattgcc agctcgggcg agacccgcgc cctgccgctc gcatcctcga tctcgaacgg
     2941 ggaaaacagc gtggtggtct tgacgatctc ggtcttgcgc ttgcagcagg aggtcttgcg
     3001 atgggagtca tattcgaaga actcgtaatc gcgctccacc gtcgacacga aatgcgcgca
     3061 agcggcgttc gatagctcgc cgatcacggg cgaagggcag cggagcctgc ctttgagctc
     3121 gaccaggctc ccgggaggca gcttggcgac atgcgccgcc ggggtggtct cggtcgcccg
     3181 catcagcgcc atgtcggtgc gcgacgaccg ccagctccac aacaggaatg cggcgctcgg
     3241 caccaggagg acgccgagca acaccagcca tgccaccatc tcgcaatccc tccgccgtcc
     3301 ggcagccgcg ttcgcacgat aatcttgttg caggtcccgg ttgaggaaga attagctttt
     3361 ggccgagg
//
LOCUS       DATNAE010000134         3616 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_1329438, whole genome shotgun sequence.
ACCESSION   DATNAE010000134 DATNAE010000000
VERSION     DATNAE010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3616
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1329438"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..439)
                     /locus_tag="VN518_02920"
     CDS             complement(107..439)
                     /locus_tag="VN518_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03555.1"
                     /translation="MSAALSLDDKRLRTSVLFAVAVHSLFILFFTVFLFQHANPMGDG
                     MEMVGSSIAFMFIFLPFSLPAYLLAKTGRLLILAALLALIASFLCFGLWLEFLDELGI
                     RPAPWNAG"
     gene            complement(436..648)
                     /locus_tag="VN518_02925"
     CDS             complement(436..648)
                     /locus_tag="VN518_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011663491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HXE03556.1"
                     /translation="MRVRIDRGADAVYLNLTDRPIEGSEEVADGIVVDYDAQGRIVGV
                     EILDASKRTDDPEVLKQFSFDLSSIT"
     gene            complement(804..1574)
                     /gene="tatC"
                     /locus_tag="VN518_02930"
     CDS             complement(804..1574)
                     /gene="tatC"
                     /locus_tag="VN518_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HXE03557.1"
                     /translation="MEHLIELRRRLIWSLGAVAIAFAVCFYFSRPLYNLLLWPYRLAA
                     GVDTPIEMIYTAPQEFFFTEMKLALFGAIFIAFPVIASQIYMFVAPGLYRSERKAFLP
                     FLAATPILFLLGASLVYFVVMPLAMTFFLSMQQSGDNQVHIQLTARVSEYLSLIMTLI
                     LAFGICFQLPVLLTLLARAGLITAAQLKAKRRIAILIAFIVAAVLTPPDPLSQISLAL
                     PTIALYELSIYAVKLAEKRHAASTASAKPAAKPAGKPA"
     gene            complement(1619..2032)
                     /gene="tatB"
                     /locus_tag="VN518_02935"
     CDS             complement(1619..2032)
                     /gene="tatB"
                     /locus_tag="VN518_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009030312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HXE03558.1"
                     /translation="MFDIGWSELLVIGVVAIIVVGPKELPRLMRTFGHYLGKVRHMAA
                     DFQRQFEEAVRDSEIDEVRKAMQDFHAEVSDVTPRGTVDRPLMMPSPAAPAPAPAEAA
                     LPAPKPKPKPRAKKPAAAKSKTPKPRAKKREEGAS"
     gene            complement(2103..2369)
                     /locus_tag="VN518_02940"
     CDS             complement(2103..2369)
                     /locus_tag="VN518_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HXE03559.1"
                     /translation="MGFGSIWHWLLVILVLVLLFGAGKISGLMGDLAKGIKSFKKEMA
                     EGDGEPAPEPESAPAPKVIEHKARPAAKAKTAPRPKTPRTPRAG"
     gene            complement(2466..3599)
                     /locus_tag="VN518_02945"
     CDS             complement(2466..3599)
                     /locus_tag="VN518_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012707926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXE03560.1"
                     /translation="MKAGRSGRESKRAPSERGRAGVTFAARLTFEDVSKRYGESLALD
                     HVSLDLAPGEVLCLLGPSGCGKTTLLRIAAGVERPTSGCVLLDGQEVAGPDRFVPPEK
                     RGVGLMFQDFALFPHLTILQNVAFGLRSLTRAEANAEALAALARVGLAHYANEYPHIL
                     SGGEQQRVALARAIAPRPSVLLMDEPFSGLDPRLREKIREETLAILHETRATCIVVTH
                     DAEEAMRMGDRVALLRAGHVVQTGRALDLYRAPRDILAARTFSDLNEVPASIENGSAR
                     TVLGSFPAGNLADGEDAIVCVRQRGVRLLAAGEGILARVLDLRFLGDVALVEMAVQGL
                     DASLFARVRESDAPEVGSEIGIGVDEGAVLVFEENGNRLNGVK"
BASE COUNT          668 a         1127 c         1233 g          588 t
ORIGIN      
        1 cggcggtaat agatcgccca cagccgcggc acgatgaggt aggcagcgac ggcccaagcg
       61 agcgtgatca gaacgagggt cttggtatcg ggcagatgca aggttgctat cccgcgttcc
      121 acggcgccgg ccggatgccg agttcgtcga gaaattcgag ccaaaggccg aaacagagga
      181 aggacgcgat cagcgcgaga agcgccgcga ggatcagcaa gcgaccggtc ttggcgagga
      241 gataggcggg gagactgaag ggcaggaaga tgaacatgaa ggcgatgctg gagccgacca
      301 tctccatgcc gtcgcccatg ggattggcgt gctggaacag aaacacggta aagaagagga
      361 tgaacaggct gtggacggcg acggcgaaga gcacgctcgt gcgaagccgc ttgtcgtcga
      421 gggagagtgc cgcgctcaag tgatgctgga caaatcgaaa ctgaactgct tcaggacctc
      481 cgggtcgtcg gtccgctttg aggcatcgag gatttccacg ccgacgatgc ggccttgcgc
      541 gtcgtaatcc acgacgatgc cgtcagcgac ctcctcgctc ccctcgatgg ggcgatcagt
      601 gaggttgaga tagaccgcgt cggctccccg gtcgattctc acgcgcatgg ctttgtcctg
      661 tcgaattctc ttccgtcatg gccgggcttg tcccggccat ctaatgatgc ccctccacag
      721 cctgtaacgt cgagcctttt tcagcgctct attgggtgcc cggcacaagg ccgggcatga
      781 cgaaaaatgt gggtggcgtt cgatcaagcc ggcttgccgg cgggcttggc agcaggtttc
      841 gccgaggcgg tcgaagcggc gtgacgcttc tcggcgagct tcaccgcgta gatcgacagc
      901 tcgtaaagcg cgatggtggg cagcgcgagc gatatttggc tcaaagggtc aggcggggtg
      961 agcacggccg ccacgatgaa ggcaatgaga atggcgatcc gccgcttggc cttgagctgc
     1021 gccgcggtga tcagcccggc gcgggcgagc agcgtcaaga gcaccggcaa ctggaagcag
     1081 atgccgaagg cgaggatcag cgtcatgatc aaagagagat attcgctcac ccgcgcggtg
     1141 agctggatat gcacctggtt gtcgccggac tgctgcatcg acaagaagaa ggtcatggcg
     1201 agcggcatga ccacgaaata gacgagcgag gcgccgagca ggaacaggat cggtgtcgcg
     1261 gccaggaacg gcaggaaggc cttccgctcg ctgcgataaa gcccaggcgc cacgaacatg
     1321 tagatttgcg aggcgatcac cgggaaggcg atgaagatgg cgccgaacag cgcaagcttc
     1381 atctcggtga agaagaattc ctgcggcgcg gtgtagatca tctcgatcgg cgtatcgacg
     1441 ccggccgcga gccggtaggg ccagagcaag agattgtaga ggggccgcga gaaatagaag
     1501 cagacggcaa acgcgatagc gaccgcgcca agcgaccaga tcagcctgcg gcggagctcg
     1561 atgagatgct ccatcaacgg cgccttggtg gcgtcgatgt ccttttgatc ctggtcgctc
     1621 acgacgcgcc ctcctcgcgc ttcttggcgc gtggcttggg cgtcttgctt ttggctgcgg
     1681 cgggcttctt cgcccgcggc ttgggcttag gcttgggcgc gggcagcgcc gcttcggccg
     1741 gcgcaggtgc gggcgcggct gggctaggca tcatcagagg tctgtcgacg gttccgcgag
     1801 gagtcacatc ggacacctcc gcatgaaagt cctgcatggc tttgcgcacc tcgtcgatct
     1861 cgctgtcgcg caccgcctcc tcgaactggc gctggaagtc ggcggccatg tgcctgacct
     1921 tgccgaggta atggccgaag gtgcgcatca gccgcggcaa ctccttgggg ccgaccacga
     1981 tgatcgccac cacgccgatg accaggagct cagaccaacc gatgtcgaac ataggcgcta
     2041 atttcgccgg aagtgtcgca ttgtcttgac cgcccgcgag ccctcagcgc atgggcaagg
     2101 agctagcccg ccctcggcgt ccgcggcgtc ttggggcggg gcgcggtctt cgccttggct
     2161 gcgggtcgcg ccttgtgctc gatcactttc ggggccggcg ccgactcggg ctctggcgcg
     2221 ggctcgccgt cgccctcggc catctccttc ttgaaactct tgatgccctt ggcgaggtcg
     2281 cccatcaggc ccgaaatctt gcctgcgccg aacaggagca cgagcacgag aatgacgaga
     2341 agccagtgcc agatgctgcc gaaacccatg aaaatctccc tgcccccagc gagccggttc
     2401 tgcgaggcac tatggcagca aagcgcgggc gctcgcaagc cgctggcgtc gcgcgcgcgg
     2461 gccgcctatt tcacgccatt gagcctgttg ccgttctcct cgaacaccag caccgcacct
     2521 tcatcgacgc caattccgat ctcgctgccg acttccggcg cgtcgctttc ccgcacgcgg
     2581 gcgaagagcg aagcgtcgag cccctgcacc gccatctcga ccagcgccac atcgccgagg
     2641 aagcggaggt cgaggacccg cgcgaggatg ccttcgccgg cggcaagcag gcggacgccg
     2701 cgctggcgca cgcagacgat ggcgtcctcg ccgtcggcga ggttgccggc tgggaagctg
     2761 ccgagcactg tcctggcgct gccgttctcg atgctggcgg gaacctcgtt caaatcggag
     2821 aaggtgcgcg cggcgagaat gtccctgggc gcgcggtaaa ggtcgagcgc gcgcccggtc
     2881 tgcaccacgt gccctgcgcg caacagcgcc acgcggtcgc ccatgcgcat ggcctcctcg
     2941 gcgtcgtggg tgaccacgat gcaggtcgcc cgcgtctcat gcaggatggc gagcgtctcc
     3001 tcgcggatct tctccctgag gcgcggatcg agccctgaga agggctcgtc cataaggaga
     3061 acgctcggcc gaggcgcgat ggcgcgggca agtgccacgc gctgctgctc gccgccggag
     3121 aggatgtgcg gatattcatt ggcgtagtga gcgagcccga cgcgcgcgag cgcggcaagc
     3181 gcctcggcat ttgcctcggc tctggtcagc gagcggagcc caaaggcgac gttctgcagg
     3241 atggtcagat gcgggaacag cgcgaagtcc tggaacatca gcccgacgcc gcgcttctcc
     3301 ggcgggacga agcgatcggg cccggcaacc tcctggccgt cgagcagcac gcacccggag
     3361 gtggggcgct cgacgccggc agcgatcctg agcagcgtgg tcttgccgca gcctgacggc
     3421 ccgagcaggc acagcacctc gccgggggcc agatcgagcg agacatggtc gagcgcgagg
     3481 ctctcgccgt agcgtttgga gacatcctca aaagtgaggc gcgcagcgaa ggtaacgcca
     3541 gccctgcccc gctcgctcgg cgcacgcttc gattcgcgcc cgcttctgcc ggctttcaag
     3601 aacccaagca cgaggc
//
LOCUS       DATNAE010000135         2014 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3737943, whole genome shotgun sequence.
ACCESSION   DATNAE010000135 DATNAE010000000
VERSION     DATNAE010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3737943"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1238)
                     /locus_tag="VN518_02950"
     CDS             complement(<1..1238)
                     /locus_tag="VN518_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HXE03561.1"
                     /translation="MNDTTQTSATEQAASSELKLPDRALIILPTRNAVLFPGVVAPLG
                     LGRPQSIAGAQAAARADKPIGVVLQKDASVDTPGPEHLHRVGTVAEILRYVTTPDGNH
                     HIVSRGARRFRITEFLTGYPFLVALVDEVGEAEVYSTEIAARMHQLKERAREAISLLP
                     AIPKELADAIDQIQSPSALADFVANASDLTPAEKQELLETFDVQERLDKLLRILAQQI
                     EVLRLSKQIGEQTQESLQSRQREHILREQLRQIQKELGEGEGSGAEIAELREAIEKAK
                     MPEEAEKQALKELARLERTPEAAAEHSMIRSYLDWLIELPWSKLSEERIDIAEARRIL
                     DEDHYGLPKIKQRILEFLAVRKLNPEGKSPILCFVGPPGVGKTSLGQSIARATGRKFV
                     RLSLGGLHDEAEIRGHRRTY"
     gene            complement(1252..1656)
                     /locus_tag="VN518_02955"
     CDS             complement(1252..1656)
                     /locus_tag="VN518_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HXE03562.1"
                     /translation="MTRDPRNLMWAEACALLDRAEQLHRQFFEPAREGGRHARWEPPI
                     DMFETERQLRIIVALPGVVPEAVQVQTEGGILTIVGSRAMPSCGENTKIVRLEIPYGS
                     FERRIALQLNRLRLDQRELVNGCLVLTFVKLG"
BASE COUNT          350 a          625 c          674 g          365 t
ORIGIN      
        1 atataggtgc gccgatggcc gcgtatctcg gcttcgtcat gcaggccgcc aaggctcaac
       61 cgcacaaatt tgcggcccgt ggcgcgcgca atcgattggc caagcgaggt cttgcccacg
      121 ccgggaggac cgacgaagca gaggatgggg ctcttgcctt cggggttgag cttgcgcacg
      181 gcgagaaatt cgaggatgcg ctgcttgatc ttgggcaggc cgtaatggtc ctcgtcgagg
      241 atgcggcgcg cttcggcgat gtcgatgcgc tcctcggaca gcttcgacca cggcaactcg
      301 atcagccagt cgaggtagct gcggatcatg gagtgctcgg cggccgcctc aggcgtgcgc
      361 tcaagccgcg ccagctcctt cagcgcctgc ttctcggcct cctcaggcat cttggccttc
      421 tcgatcgctt cgcgcagctc ggcgatctcg gcgcctgagc cttcgccctc gccaagctct
      481 ttctgaatct gacgaagctg ctcgcgcaag atgtgctcgc gctggcggct ttgcagcgat
      541 tcctgcgtct gctcgccgat ctgcttggac aggcgcaaga cttcgatctg ctgggcgagg
      601 attcgcaaca gcttgtccag gcgctcctgg acatcgaagg tctcgagcag ctcctgcttc
      661 tcggcaggcg tgagatctga ggcgttggcg acgaagtcgg caagcgccga gggcgactga
      721 atctgatcta tggcgtcggc aagctctttg gggattgccg gcagcagcga tatcgcctcg
      781 cgtgcccgct ctttcagctg atgcatgcgg gcggcgatct cggtcgaata gacctcggcc
      841 tcgcccacct catcgacaag ggcgacgagg aagggataac cggtgagaaa ctcggtgatg
      901 cggaaacggc gggcaccgcg cgagacgatg tgatgattgc cgtccggcgt ggtcacgtaa
      961 cgcaggattt cggccaccgt gccgacgcga tgcagatgct caggccccgg cgtgtcgacc
     1021 gaagcgtcct tctgcagcac cacgccgatc ggcttgtcgg cgcgcgcggc ggcctgggcc
     1081 ccggcgatag attgcgggcg gccgagaccg agcggcgcca ccacgccggg gaagagcacg
     1141 gcgttgcgcg tcggcaggat gatcaaagcg cgatcgggca gcttgagctc agaggaggct
     1201 gcctgttcgg tcgcggaagt ttgggtcgta tcgttcatga attgaacctg ctcagccaag
     1261 cttgacaaag gtgagcacga ggcaaccgtt cacgagctca cgttgatcaa gccgcaagcg
     1321 gttgagttgc agtgcgatgc ggcgctcgaa gctgccatag ggaatctcca gccgcacgat
     1381 cttggtgttc tcgccgcacg aaggcatggc ccggcttccg acgatggtca ggattccgcc
     1441 ttcggtctgc acctgcacgg cttccggtac gacgccgggg agcgccacga tgatgcggag
     1501 ctgccgctca gtctcgaaca tgtcgatcgg cggttcccag cgcgcgtggc ggccgccttc
     1561 gcgggcgggc tcgaagaatt gccggtggag ctgctcggcc ctgtccagca gggcgcaagc
     1621 ctcggcccac atcagatttc ggggatctcg cgtcacgcaa gcctcgatga gttaccgcag
     1681 ccgatcttac ccgcccggcc ccatcgtgtg aaaataacga gggccaatag gccggcgcaa
     1741 tcaagagatc gcagaaaagg gttgaaggga cgctatatat tggtgccctg cacccctcct
     1801 gcaagtgcct gtcggggctg cgctagggcg ccgcagcgcc ttcggggatg gcttggcaat
     1861 cggcgagccg cacaagctcg ctttcgcctg gaaagagagc gagcgcgcaa accggcgagg
     1921 atgccggtac tgccgcgacg cctttccagc cgacgaggcc gagggatcgc gccgcggcct
     1981 gttccacatc tggacggcgc gcgacgaagg ctcc
//
LOCUS       DATNAE010000136         2147 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_429373, whole genome shotgun sequence.
ACCESSION   DATNAE010000136 DATNAE010000000
VERSION     DATNAE010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429373"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VN518_02960"
     CDS             <1..199
                     /locus_tag="VN518_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03563.1"
                     /translation="RMPDYEEILSFLAANPPELAAERELHASLLELIAKCLRSDSLAA
                     EEDEFLPEGLPACRLRWMDLN"
     gene            345..1037
                     /locus_tag="VN518_02965"
     CDS             345..1037
                     /locus_tag="VN518_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HXE03564.1"
                     /translation="MRDSVAVKHSAEPISAEDIVRSFIQSIDTAKRSEQPYRHWTLAA
                     CLPSDAVDDILALPFDPPSLDGVSGKRELHNNTRKYFDVENRARFPICEAVAQAFQDK
                     RVTSHIEKMFVTDLKGTYLRIEFAQDIDGFWLEPHSDLGVKLFTMLLYLSKDPSHKDL
                     GTDIYDANKQRVGRSPFAPNFAMIFVPADNTFHGFERRPIKGVRTSLIINYVSNEWRA
                     REQLAFPETPLT"
     gene            1108..1992
                     /locus_tag="VN518_02970"
     CDS             1108..1992
                     /locus_tag="VN518_02970"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HXE03565.1"
                     /translation="MSKLAWIGLGTMGFPMAGHLKTRGGHAVTVFNRTRAKADAWVAK
                     FGGNAAPSPAEAADGADFVFSCVGNDDDLRAVTLGQDGAFQALRRGAVFVDHTTASAG
                     IARELHAAAKERGAGALDAPVSGGQSGAENGTLTVMVGGDAADFDKAKPVIEAYARAV
                     NLIGATGSGQLTKMVNQICIGGLIEALAEALHFAKAAGLDVEKVVETICQGAAQSWQM
                     DNRWKTMLDGKFDFGFAVDWVRKDFALVFEEARANGAELPVTALVDQFYAEIQAMGGG
                     RWDTSSLIARLEPRKPKG"
     gene            complement(1998..>2147)
                     /locus_tag="VN518_02975"
     CDS             complement(1998..>2147)
                     /locus_tag="VN518_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007629545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HXE03566.1"
                     /translation="LRVVVPDIERYDAFYKKLISKIELSDVSSAFAMEQIKYSTALPL
                     DYATE"
BASE COUNT          374 a          703 c          681 g          389 t
ORIGIN      
        1 ccgaatgcct gattacgagg agatcctctc cttccttgcc gcgaacccgc ccgagcttgc
       61 cgccgagcgc gagctgcatg cctcgctgct ggagctgatc gccaagtgct tgcgatccga
      121 ctcgctcgcc gccgaggagg atgagttttt gccggaaggc ctgcccgcgt gccggctgcg
      181 ttggatggat ttgaactgac gcccagcccg gctgatttgt gtctgcgtcc tgcgacccat
      241 ggcatttgcg cgcgccgcct atgggtgcta tctgaacttt aggtgggccg cctgggcgct
      301 ggatggccag cattttcgca cttttggggg gagttagcct catcatgagg gacagcgtcg
      361 ccgtcaagca ttcggccgag ccgatttcag ccgaggatat cgtccgcagc ttcattcaga
      421 gcatcgatac cgccaagcgc tccgagcagc cttatcgcca ctggacgctc gccgcttgtc
      481 tgccgtccga tgccgtcgac gacattctcg cgctgccctt cgacccgcct tcgctcgacg
      541 gcgtgtcggg caagcgcgag ctgcacaaca acacccgcaa atatttcgac gtcgagaacc
      601 gcgcgcgctt ccccatctgc gaggcggtgg cgcaggcctt ccaggacaag cgggtcacca
      661 gccatatcga aaaaatgttc gtgaccgacc tcaagggcac ctatctgcgc atcgaattcg
      721 cgcaagacat cgacggcttc tggctcgagc cgcacagcga tctaggcgtc aagcttttca
      781 ccatgctgct ctacctgtcg aaggacccga gccacaagga tctcggcacc gatatctacg
      841 acgccaacaa gcagcgcgtc ggccgctcgc ccttcgcacc caacttcgcc atgatcttcg
      901 tgccggcgga caacacgttt cacggcttcg agaggcggcc gatcaagggc gtgcgcacct
      961 cgctgatcat caactacgtc agcaatgagt ggcgtgcgcg cgagcagctc gcctttcccg
     1021 aaacgccact cacctaggct tttccgcgca ccggggtagc cctggattcc ggcttgcgcc
     1081 ggaatgacga agccggtgag attgcccatg agcaaactcg cgtggatcgg gctcggcacc
     1141 atgggctttc ccatggccgg tcatctcaag acgcgtggcg gccacgcggt gacggtgttc
     1201 aatcgcaccc gcgccaaggc cgatgcctgg gtcgccaagt ttggcgggaa cgcggcgccc
     1261 tccccggccg aggccgcgga cggcgccgac ttcgtcttta gctgcgtcgg caatgacgac
     1321 gaccttcgcg cggtgacgct ggggcaagac ggcgcgtttc aggcgctgcg ccgcggcgcc
     1381 gtgttcgtcg accacaccac ggcctcggcc ggcatcgccc gcgagctgca tgcggcagcc
     1441 aaggagcgcg gcgccggcgc gctcgacgcg ccggtctccg gcgggcaatc cggcgccgag
     1501 aacggcacgc tcaccgtgat ggtcggcggc gacgcggcgg atttcgacaa ggccaagccg
     1561 gtgatcgagg cctatgcccg cgccgtcaat ctgatcgggg cgacgggatc gggccagctc
     1621 accaagatgg tcaatcagat ctgcatcggc ggcctgatcg aggcgctcgc cgaggcgctg
     1681 cattttgcca aagccgccgg cctcgatgtc gagaaagtgg tcgagaccat ttgccaaggc
     1741 gcggctcagt cgtggcaaat ggacaatcgc tggaagacga tgctcgacgg caaattcgat
     1801 ttcggcttcg ccgtcgattg ggtgcgcaag gatttcgcgt tggtgttcga ggaggcgcgg
     1861 gcgaacggcg ccgagctccc ggtgacggcg ctcgtcgacc aattctatgc cgagatccag
     1921 gcgatgggcg gcggccgctg ggacacctcg agcctgatcg cgcggctcga gccccggaag
     1981 ccgaaaggct aagcccccta ttccgtcgcg taatcgagcg gcagcgcggt cgagtatttg
     2041 atctgctcca tggcgaaggc cgagctcacg tctgacagct cgatcttgct gatgagcttc
     2101 ttgtagaagg cgtcgtagcg ctcgatgtcg gggaccacga cccgcaa
//
LOCUS       DATNAE010000137         3152 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2234838, whole genome shotgun sequence.
ACCESSION   DATNAE010000137 DATNAE010000000
VERSION     DATNAE010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2234838"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1072)
                     /locus_tag="VN518_02980"
     CDS             complement(<1..1072)
                     /locus_tag="VN518_02980"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HXE03567.1"
                     /translation="MFRIVAAALILLVSAWSPANADAKRVALVIGMSDYQHLSKLPNP
                     VPDAKAIAAEFKSHGFEVSEYYNLDRAGLLDALEKFERDATGAEVALVYYAGHGMSVQ
                     GKNLLAPTDMEITCEDKTTIRSVDLQQLYKAAGPAPQQIVMLDACRNDPFPQCPTRGT
                     GQGSGFRGLSLLGEADRTVVTANATGSGQLAADGDPGAHSPFATALLRNFTENPRLYF
                     GDLLNKTATEVKLATRTQTPEVSFQGGSPKACLDVEGCGLAGPLPADTVLTDPATVAE
                     ARSALQQLGFIANASRGGDDEILADAIKRFQAKAGLTPDGQLTASLLAVMRVTKTQFA
                     ALPVPGKPAVPGIGAGPLEHEVG"
     gene            1260..1802
                     /locus_tag="VN518_02985"
     CDS             1260..1802
                     /locus_tag="VN518_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03568.1"
                     /translation="MQASLSRLAQVALLGAALALVPLFARAEPKAPSEEENADAAFAA
                     SYIGKNYDGDLDIEGWDDLGGGLIAAPIFIHQYQREDGTYLVITSRQLAKESKDTPAN
                     YEVVDALIVPPPQAGVEFTISCVQGKDETLRFIGEAKGPESKEWWTEVRRAWEIALDT
                     GKISSTKTKGVRCTNVSWGQ"
     gene            1816..2097
                     /locus_tag="VN518_02990"
     CDS             1816..2097
                     /locus_tag="VN518_02990"
                     /inference="COORDINATES: protein motif:HMM:NF016162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HXE03569.1"
                     /translation="MKARLKDRLIAVSDDIVAHGGYHYFPISVVRMELLEKAPKTESD
                     HDCPHGVQFYDVVIEGERHPRAAWIYETPRPSMQHVADRFGFWEDVEVG"
     gene            2098..2580
                     /locus_tag="VN518_02995"
     CDS             2098..2580
                     /locus_tag="VN518_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthrone oxygenase family protein"
                     /protein_id="HXE03570.1"
                     /translation="MTLLALTFLAALGSGLIAGFFLAFSACVMAALGRIPPASGISAM
                     QSVNVVVLNPVFLGTFFGTAALSLVLAVASLMNWAEPAAFYLLVGSLLYLIGSILVTM
                     ICNVPLNDRLAAVKPESAEGNAVWTHYLSAWTAWNHVRTVASLAAASCFIVATAQQWS
                     "
     gene            complement(2577..>3152)
                     /locus_tag="VN518_03000"
     CDS             complement(2577..>3152)
                     /locus_tag="VN518_03000"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HXE03571.1"
                     /translation="FQRAMSGGSAIAAQSILASYDFSGIERLADLGGGHGLLLASVLQ
                     KYSAMQGVLFDLPEVVAGVPEDRLASCGGRLRVEAGSFFERVPQGCDAYMMKHIIHDW
                     SDAHCRNILRLVRAGLPPHGRLLVCEQVMPEGPGPAPAKMLDIEMLVMTVGGKERTPR
                     EFAELFASAGLKLGRVVTTPMPTCVIEALPA"
BASE COUNT          548 a         1019 c         1017 g          568 t
ORIGIN      
        1 agccgacctc atgctctagc ggccccgcgc cgatgccggg cactgcgggc ttccccggca
       61 ctggcagcgc tgcgaattgc gtcttggtga cacgcatcac ggcaagcagg ctcgcggtga
      121 gctgcccgtc gggggtcagc ccggccttgg cctgaaagcg cttgatggcg tccgcgagga
      181 tctcgtcgtc tccgcccctg ctcgcgttgg cgatgaagcc gagctgctgc aacgccgagc
      241 gagcctcggc gaccgtcgcc gggtcggtga gcacggtatc ggccggcagg gggccggcga
      301 ggccgcaccc ctctacatcg aggcaggctt tcggcgagcc gccctggaag ctgacctctg
      361 gggtttgcgt gcgcgtcgcg agcttcacct cggtcgcggt cttgttcagc aaatcgccga
      421 aatagaggcg tggattttcg gtgaagtttc tgagcagggc ggtcgcgaag ggcgagtgcg
      481 cgcctggatc gccgtccgcc gcgagctggc ccgacccggt ggcattggca gtgaccaccg
      541 tgcggtcagc ctcgccgagc agagagagac cgcgaaagcc gctgccttga cctgtccccc
      601 gcgtcgggca ttgcggaaac ggatcgttgc ggcaggcgtc gagcatcacg atctgctgcg
      661 gcgccggtcc ggccgcctta tacaactgct gaaggtcgac cgagcggatc gtggtcttgt
      721 cttcgcatgt gatctccata tcggtcggcg cgagcaggtt cttgccttgg accgacatac
      781 cgtggcctgc gtagtagacc agcgcgacct cggctccggt cgcgtcacgt tcgaatttct
      841 ccaaggcgtc gagcaggccg gcgcggtcga ggttgtaata ttccgacacc tcgaagccgt
      901 ggctcttgaa ctccgcggcg atggccttgg cgtccggcac cggattgggc agcttggaca
      961 gatgctggta gtcgctcatg ccaatgacga gggcgacacg cttggcgtcg gcattcgcgg
     1021 gcgaccacgc cgagacgagg aggatgagag ctgcggcaac gatacgaaac atggcggtgc
     1081 tcgcctttcc gttcccgcaa gctgagccct cggcaagccg cttgggtgag gaaatttagc
     1141 acccgcggcg gcccacgcga agggcatggc tcacgccctg ttccgctttt gccatgattg
     1201 tgaatagact tgagattgcc gcgaccccta aatcccggcg cttttccgtg gatgccgcca
     1261 tgcaagccag cctttcccgt ctcgctcagg tcgccctgct tggcgcggct ctcgcgcttg
     1321 tgccgctctt cgcgcgcgcc gagccaaagg ctccgagcga ggaggaaaac gccgacgccg
     1381 ccttcgccgc gagctacatc ggcaagaact atgacggcga tctcgacatc gaaggttggg
     1441 acgatcttgg cggcggcctc atcgccgcgc cgatcttcat tcaccaatat cagcgcgagg
     1501 acgggaccta tctcgtgatc accagccgcc agctcgccaa ggagtcgaaa gacacgccgg
     1561 cgaattacga ggtggtcgac gcgctgatcg tgccgccgcc gcaggccggc gtcgagttca
     1621 ccatctcctg cgtgcagggc aaggacgaga cgctcagatt catcggcgag gccaaggggc
     1681 ctgagagcaa ggaatggtgg accgaggtgc ggcgcgcctg ggagatcgcg ctcgacaccg
     1741 gcaagatctc ctcgaccaag accaagggcg tccgctgcac caacgtgtcc tggggccagt
     1801 aggagagatt gacccatgaa ggcaaggctg aaggaccgcc tcatcgccgt gagcgacgac
     1861 atcgtcgctc acggtggcta ccactacttc ccgatctcgg tggtgcgcat ggagttgttg
     1921 gaaaaagcgc cgaagactga gtcggatcac gattgcccgc atggcgttca gttctacgac
     1981 gtggtgatcg agggggagcg acatccccgt gccgcctgga tctacgagac gccgcgtcct
     2041 tcgatgcaac atgtggcgga ccgcttcggc ttctgggaag acgtcgaggt tggttgagtg
     2101 accctcctcg ccctgacatt tctcgcagcg ctcggctctg gcctcatcgc cggttttttc
     2161 ctcgcctttt cggcatgcgt catggcggct cttgggcgca ttccgccggc atcgggaatt
     2221 tccgccatgc aatcggtcaa tgtcgtggtg ctgaatccgg tgtttctggg aaccttcttc
     2281 ggaacggccg cgctctctct cgtgctcgcc gtcgcgagcc ttatgaactg ggccgagccc
     2341 gccgccttct atttgctcgt aggcagcctg ctctacctca taggctccat tctcgtgacc
     2401 atgatctgca acgtgccgct caacgacagg ctcgccgccg tgaagcctga gagcgccgag
     2461 ggcaacgcgg tatggacgca ctacctctca gcgtggacgg cctggaacca tgtgcgcacc
     2521 gtagcgtcgc tcgcagcggc ttcatgcttc attgtggcga cagcacagca gtggagctag
     2581 gccggaagcg cctcgatcac gcaggtgggc atcggcgtcg tcaccacgcg gccgagcttg
     2641 agccccgccg aggcaaacag ctcggcgaac tcgcgcggcg tgcgctcctt gccgccgacg
     2701 gtcatcacca gcatctcgat atcgagcatc ttggccggcg ccggacctgg cccctccggc
     2761 atgacctgct cgcagacaag caagcggcca tgcggaggca accccgcgcg gacgagccgc
     2821 aggatgttgc ggcaatgcgc atcgctccaa tcgtggatga tgtgcttcat catataggcg
     2881 tcgcaccctt gcggcacgcg ctcgaagaag cttccagcct cgacgcgcaa gcggccgccg
     2941 caggaggcga gacgatcctc cggcacgcca gcgaccacct cgggaagatc gaacagcaca
     3001 ccctgcatcg cagagtattt ttgcaggacc gaggcaagca ggagcccgtg ccctccgcca
     3061 aggtcggcaa gacgctcgat gccggaaaaa tcgtaagacg cgaggatcga ttgcgccgcg
     3121 atggccgagc cgcctgacat cgcccgctgg aa
//
LOCUS       DATNAE010000138         3576 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2538007, whole genome shotgun sequence.
ACCESSION   DATNAE010000138 DATNAE010000000
VERSION     DATNAE010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2538007"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(310..1014)
                     /gene="lexA"
                     /locus_tag="VN518_03005"
     CDS             complement(310..1014)
                     /gene="lexA"
                     /locus_tag="VN518_03005"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HXE03572.1"
                     /translation="MLTTKQKELLMFIHERLKETGVPPSFDEMKEALDLKSKSGIHRL
                     ITALEERGFVRRLPHRARAMEIVRLPDSMPQAFGAGRARGFSPSVIEGSLGRVPQVAE
                     GESLPQTVSVPVMGRIAAGVPIEAIQTRLDTVEVPPDLLTKGEHYALEVKGDSMIDAG
                     ILDGDTIILRKTEDASNGDIVVALVDEEEATLKRLRRRGDSIALEAANAAYETRIFGP
                     DRVRVQGRLVGLIRRY"
     gene            complement(1192..2388)
                     /gene="glp"
                     /locus_tag="VN518_03010"
     CDS             complement(1192..2388)
                     /gene="glp"
                     /locus_tag="VN518_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HXE03573.1"
                     /translation="MALLSVTEALARVTEGLAPLDAERVPLAECRGRVLAEDLAASLT
                     QPPFDASAMDGYAVRAEDVAALPATLRLIGESLAGSGYDGEVGRGEAVRIFTGAPVPK
                     GADTVVIQEDTDAAPGVVIVKEAAPHRHIRPRGQDFTAGEVLMRAGVRLGPRELMLAA
                     AMNHAELPVRRKPKVAILATGDEVVPPGTELARDEVVSSVPAGLAALIEAKGGEPMSL
                     GIAKDTAESLITLARTGSAAEILVTIGGASVGERDLVSAALRSEGLELDFWKIAMRPG
                     KPLLYGRLGTQRVLGLPGNPVSALICGLVFLVPMLHRLLGLREGDGHVEATLGQAVEA
                     NGPRQHYMRATWQWEAGERRATPLPAQDSSLTADFARADCLIVRPPDAPALPRGARVT
                     IIPLDD"
     gene            complement(2395..2874)
                     /gene="moaC"
                     /locus_tag="VN518_03015"
     CDS             complement(2395..2874)
                     /gene="moaC"
                     /locus_tag="VN518_03015"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008875897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HXE03574.1"
                     /translation="MTARRLSHLNEAGEARMVDVSEKAVTERRATAEGFVSMAPETLD
                     LILSGDAPKGDVLVTARIAGIVAAKRTHELIPLCHPLPLTQVTVDFAPSRDPAGIRVE
                     ATAKVEAKTGVEMEALTAVSVACLTIYDMVKAVDRAMTFSGIRLIEKTGGRSGPFRR"
     gene            complement(2871..>3576)
                     /gene="trpC"
                     /locus_tag="VN518_03020"
     CDS             complement(2871..>3576)
                     /gene="trpC"
                     /locus_tag="VN518_03020"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HXE03575.1"
                     /translation="PFADAIAARLAESEFALIAEIKKASPSKGLIRADFDPPALARAY
                     QAGGATCLSVLTDMPSFQGRPEFLTTAREATSLPALRKDFMVDPYQVLEARALGADCI
                     LIIMAAVDDATARELTTAAHDLGMDVLAEVHDAAELDRALALDTGLIGINNRDLKTFV
                     TDLAVTEALAPRVPKDHIVIAESGIFTHADLERLAGIGVGAFLVGESLMRQADVAEAT
                     RTLLTGVPAKQREAAQ"
BASE COUNT          606 a         1181 c         1185 g          604 t
ORIGIN      
        1 acgacgatag agctcatggg tcaggcaaaa ccttagttgg aattcagacg gaactcgatg
       61 ccctccccgc actcttcgcc ggcggcatcc tcatctattt cggcctccca gaagagcctc
      121 gcctgatgcg cttcgacgac aatccagatg agtaacttcc ccctctcccc tgtggggaga
      181 gggttggggt gagggggttc gtaattctca gaactctgaa accccctcac ccgcgctgtc
      241 gctggcaacg acagcgcgac ctctccccgc aggggagagg tgatcagaac ctcgaagtga
      301 gggcgaaatc taataccggc ggatgaggcc gacgagccgc ccctgcacgc gcacgcggtc
      361 tgggccgaag atgcgcgtct cataggccgc gttggcggct tcaagcgcga tcgagtcgcc
      421 ccgccggcgc aagcgcttca gcgtcgcttc ctcttcatcc acgagcgcca cgacgatgtc
      481 gccattgctg gcgtcctcgg tttttctgag gatgatggtg tcgccgtcga gaatgccggc
      541 gtcgatcatg gaatcgcctt tcacctcgag cgcgtaatgc tcgcccttgg tgagcaaatc
      601 gggcggcacc tcgaccgtgt cgaggcgcgt ctggatggcc tcgatcggca cgccggcggc
      661 gatccggccc atgaccggca cgctcaccgt ctggggcagg ctctcgcctt cggccacctg
      721 aggcacgcgg ccaaggcttc cttcgatcac gcttggcgag aaaccgcggg cccggccggc
      781 cccgaacgct tggggcatgg aatccggcag ccgcacgatc tccatggcgc gggcccggtg
      841 cggcaggcgc cgcacgaagc cgcgctcctc gagcgccgtg atcaggcggt gaatgccgga
      901 tttcgatttg agatccagag cttccttcat ctcgtcgaag gaggggggca cgcccgtctc
      961 cttcaggcgc tcatggatga acatgaggag ctctttttgc ttggttgtca gcatctggtc
     1021 ccttcaggcg tccgtttggg ggcgcgatcc cgctgtacaa atcatgaaca gtttatctgt
     1081 tctatttgtg ttctgcaagc aagaatgacc ccatatctcg tgccgagaca tccagtaggg
     1141 gtaggatttc cgcgattcgg gttgccgagg gattaaccgg accgccacag atcagtcatc
     1201 gagagggatg atggtcaccc gcgcacccct aggaagggcc ggggcgtcgg gcggcctgac
     1261 gatcaggcag tcggcgcggg cgaagtcggc cgtcaaggaa ctgtcctggg cgggaagggg
     1321 ggtcgcccgg cgctcgccag cctcccattg ccacgtcgcc cgcatgtaat gctggcgggg
     1381 gccgttggcc tcgacggcct gcccaagcgt cgcctcgaca tgcccgtcgc cctcacgcag
     1441 cccaagcagg cggtgcagca tcggcaccag gaacacgagc ccgcagatca gcgccgaaac
     1501 ggggttgccg gggagcccaa gcacccgctg ggtgcccaag cggccataga gcaggggctt
     1561 gcccggccgc atggcaatct tccagaagtc gagctcgagc ccctccgagc ggagcgccgc
     1621 gctgaccagg tcgcgctcgc cgacggacgc cccgccaatg gtgacgagaa tctcggcggc
     1681 gctgccggtg cgggcaagcg tgatcaggct ctccgccgtg tccttggcaa tgccgaggct
     1741 catcggttcg ccgcccttcg cctcgatcaa cgcggcaagt ccggccggga ccgaggagac
     1801 gacctcatcg cgcgcaagct cggtgcccgg cggcaccacc tcatcgccgg tggcgaggat
     1861 agcgactttc ggcttgcggc ggacgggaag ctcggcgtgg ttcatggcgg cggcaagcat
     1921 cagctcgcgc ggaccgagcc tcacgcctgc gcgcatcagc acctcgcctg cggtgaagtc
     1981 ctggccgcgc ggcctgatat ggcgatgcgg cgccgcctcc ttgacgatca ccacgcctgg
     2041 ggcggcgtcg gtgtcctcct gaatgacgac ggtgtcggcg cccttcggca ccggagcgcc
     2101 ggtgaagatg cgcaccgctt cgccgcggcc gacctcgccg tcatagccgc ttcccgccaa
     2161 ggattcgccg atcagcctga gcgtcgccgg cagcgcggcg acatcctcgg ccctgacggc
     2221 atagccgtcc atggccgagg catcgaaggg gggctgggtg agtgaggcag cgagatcttc
     2281 ggcgagaacg cgaccgcggc actcggcgag cggaacgcgc tcggcgtcga gcggcgctag
     2341 cccctccgtc acgcgggcga gagcttcagt tacgctgagg agtgccatgt aggatcagcg
     2401 gcggaacggc ccggagcggc cgccggtctt ctcgattagc ctgatgccgg agaaggtcat
     2461 ggcgcggtcg accgccttca ccatgtcgta gatggtgagg caagcgaccg acactgcggt
     2521 cagcgcttcc atctccacgc cggtcttagc ctcgaccttg gcggtcgcct cgacccggat
     2581 gccggcagga tcgcgcgacg gcgcaaagtc gactgtgact tgagtcaagg gcagggggtg
     2641 gcaaagcggg atgagctcat gcgtgcgctt ggccgccacg atgccggcga tgcgggcggt
     2701 gacgagcacg tcgcccttcg gagcatctcc gctcaagatc aggtcgagcg tctcaggcgc
     2761 catggaaacg aaaccctcgg ccgtggccct ccgctcggtg accgccttct ccgagacatc
     2821 gaccatgcgc gcctcgcccg cctcgttcag atggcttaag cgccgcgcgg tcattgcgcc
     2881 gcctcgcgtt gcttcgccgg aacaccggtg agtagcgtcc gcgtggcctc ggccacatct
     2941 gcctggcgca tcagactctc gccaacgaga aaagcgccga cgccgatgcc agcgagcctc
     3001 tccagatcgg catgcgtgaa gatgccgctt tcggcgatga cgatgtggtc cttgggcacg
     3061 cgaggtgcca gcgcctcggt taccgcgaga tcggtgacga aggtcttgag gtctcggttg
     3121 ttgatgccga tgagcccggt gtcgagtgca agtgcgcggt cgagctccgc cgcatcgtgc
     3181 acctcggcga gcacgtccat gccgagatca tgcgcggcag tcgtaagctc acgcgccgtg
     3241 gcgtcgtcaa cggccgccat gatgatcagg atgcagtcgg cgccaagagc ccgcgcctcg
     3301 agcacctgat agggatcgac catgaaatcc ttgcgcagcg ccggcaggct cgtcgcctct
     3361 cgcgccgtcg tcaaaaactc aggccgtccc tgaaacgatg gcatgtctgt cagcacagag
     3421 agacacgtcg cgccgccggc ctgataggcg cgggcgagcg ctggaggatc gaaatcggcg
     3481 cggatcagcc ccttcgacgg gcttgccttc ttgatctcgg cgatcagcgc aaattcggac
     3541 tcggcaagcc gcgcagcgat ggcatcggca aacggc
//
LOCUS       DATNAE010000139         5562 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_1030699, whole genome shotgun sequence.
ACCESSION   DATNAE010000139 DATNAE010000000
VERSION     DATNAE010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5562
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1030699"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="VN518_03025"
     CDS             complement(<1..617)
                     /locus_tag="VN518_03025"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013502514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HXE03576.1"
                     /translation="MTKPLPIRLGINIDHVATLRNARGGHHPDPLRAAEVAIEAGADN
                     ITAHLREDRRHIRDDDIERLITNLTVLLNLEMAATPEMLAIALKTKPHAACLVPEKRT
                     ERTTEGGLDVYDQANLLAPYVGKLGDAGIQVSLFIAPELREIEAAARIGAAAIEIHVG
                     PWCDALAEGRTREADAEWRRIVAGASAAHELGLEVHAGHGLDYSTA"
     gene            complement(614..1186)
                     /locus_tag="VN518_03030"
     CDS             complement(614..1186)
                     /locus_tag="VN518_03030"
                     /inference="COORDINATES: protein motif:HMM:NF021363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HXE03577.1"
                     /translation="MLFKRRQTESLLERVRIHLWPRRSWSRSGRYVVYRLRRLSATPH
                     AIALGFAAGSFAAVTPYIGTHMVMAALLAWIIGGSIVASILGTFFGNPLTYPFLWYAS
                     YEVGNVLLGGKATKKTIDLSHGVFQSSFDHLWPILKPMSLGCVPVGLAVATACYFLVR
                     PMVDAYQHRRRRELHLRHRRPVVGTPGAAP"
     gene            complement(1311..3551)
                     /locus_tag="VN518_03035"
     CDS             complement(1311..3551)
                     /locus_tag="VN518_03035"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019444226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HXE03578.1"
                     /translation="MTRQYELVERVKSYEPDADEALLNRAYVYAMKAHGNQKRASGAP
                     FFSHPLEVAAILAEMRLDDATIATALLHDTIEDTGATRSEIDALFGPEIGSLVDGLTK
                     IKKLDLVTKKAEQAENFRKLLLAISSDIRVLLVKFADRLHNMRTLGAMKPAKRRIIAE
                     ETMEIYAPLAGRMGMQWLREELEDHAFRWLNPEGYAAVVDRLKMLRERNQGLIEEIEN
                     ALRKKLEEAKVPAVVAFREKKPYAIWSKMERKQISLEQLSDIYAFRIVVKEVEDCYRA
                     LAVVHTTWHAVPGRFKDYISSPKQNDYQSIHTTIVGPRHQRAELQLRTERMHSVAEYG
                     VAAHALYKDSANGSIPQDGVLPSHESNAYRWLRHLVDMLLEGDNPEEFLEHTKLELFQ
                     DQVFCFTPKGRLIALPRGANAIDFAYAVHTDIGNTCVGCKINGRPMPLITELRNGDEV
                     EIITSDVQAPPSAWQSLAVTGKARSAIRRATRDAARAQYGKLGREILERAFSRRGKRF
                     SEEVLAQALGRLSQKTPEDVIAAVGRGELSSADVIKAAWPEDTPSEAPKRRRKVKRAE
                     EGWFGLGKVMGLKFRWPGSSSSSEREPPAGANSIPIRGLRGDLPVSFAADGAVPGDRI
                     VGILTPGQGITIYPIHAEALKAFDEEPERWIDVTWDIDEDNAQRFPAKIAVTALNEPG
                     SLAQVAAVIGEGDGNIDNIKIMKQVPDYAEMVIDLEVWDLTHLNEILAGLRAKDVVSS
                     AVRVSG"
     gene            complement(3609..4019)
                     /gene="rpoZ"
                     /locus_tag="VN518_03040"
     CDS             complement(3609..4019)
                     /gene="rpoZ"
                     /locus_tag="VN518_03040"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HXE03579.1"
                     /translation="MARVTVEDCIDKIPNRFELVLLSGHRARTISAGAQIAVDRDNDK
                     NPVVALREIADEKIACDDLREDFIHSMQKYVEVDEPEAEAVPMLSADTRMPILGQDDQ
                     SSDNAIDKMTEEELLRRMATQAPSDGSNAGPRSR"
     gene            complement(4109..4669)
                     /gene="folK"
                     /locus_tag="VN518_03045"
     CDS             complement(4109..4669)
                     /gene="folK"
                     /locus_tag="VN518_03045"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HXE03580.1"
                     /translation="MDAQSNAHGKVLVGLGANSPGPWGSPAKTLKRALRELERRGITV
                     EAVSNLYETAAMGAARQPPYVNQVALLATSLPAPALLRLLKQIEAQAGRRGGRPWGPR
                     TLDLDIIDYKGMILNWSQSRKTMPRARVRPLVLPHPQLELRPFVLRPLLDVAPNWRHP
                     VLKLSAEALWRRVKKQGQGSVLKCLS"
     gene            4763..5371
                     /locus_tag="VN518_03050"
     CDS             4763..5371
                     /locus_tag="VN518_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003608985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HXE03581.1"
                     /translation="MHFYPTERVALFIDGANLYATAKSLGFDIDYKRLLALFGTKGQL
                     IRALYYTALAEEQEYSSIRPLIDWLDYNGYTMVTKPTKEFTDATGRRKIKGNMDIELA
                     VDAMELSEHLDHIVLFSGDGDFRSLVEALQHKGKRVSVVSTLATNPPMVADELRRQAD
                     QFIDLAHMQSEIGRDPAERAQREQRRRPEPLDEFDSEDLIET"
     gene            complement(5348..>5562)
                     /locus_tag="VN518_03055"
     CDS             complement(5348..>5562)
                     /locus_tag="VN518_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03582.1"
                     /translation="TIRSRALSGPKDGTAPLNQSGWAVLLVARKATRRGQIEQSGAGS
                     GVVARVLWARGIGLAKLRRSGFDQIL"
BASE COUNT          960 a         1750 c         1797 g         1055 t
ORIGIN      
        1 gcggtcgaat aatcgagccc atggcccgca tgcacttcaa ggccaagctc gtgcgcggcg
       61 ctggcgccag cgactatgcg ccgccactcc gcatccgcct cgcgcgtgcg cccttcggca
      121 agcgcatcgc accaagggcc gacatgaatc tcgatggccg ccgcgccgat gcgcgcagcg
      181 gcttcgatct cgcgaagctc aggcgcaatg aacagtgaga cttgaatgcc ggcgtcgccg
      241 agcttgccga cataaggagc gagcaggttg gcttggtcat acacgtcgag gccgccttcg
      301 gtggtgcgtt cggtgcgctt ctccggcacg agacaggcgg cgtgcggctt ggtcttaagc
      361 gcaatcgcga gcatctcagg cgttgccgcc atctccaggt tgaggagcac ggtcaaattc
      421 gtaatgagcc gttcgatgtc gtcatccctg atgtggcgac ggtcctcgcg cagatgcgcg
      481 gtgatgttgt cggcgccggc ctcgatcgcg acctcggccg cccgcaacgg atctggatgg
      541 tgcccgcccc gcgcattgcg cagcgttgcc acgtgatcga tgttgatgcc gaggcggatg
      601 ggaagcggct tggtcatggc gctgctcccg gcgtgccgac cacggggcgg cgatgccgca
      661 aatgaagctc acgccggcgg cggtgctgat atgcgtccac catcggcctc accaggaaat
      721 agcaggcagt cgcgacggcg aggcccaccg gcacgcagcc gagcgacatc ggcttcagga
      781 tcggccacag atgatcgaag gaggactgaa agacgccatg cgacaagtcg atcgtctttt
      841 tcgtcgcttt gccgccaagc aagacgttgc cgacttcgta gctcgcgtac cacaggaagg
      901 gataggtcag cggattgccg aagaaggtgc cgagaataga ggcgacaatg ctgccgccga
      961 tgatccaggc gagcaatgcg gccatgacca tatgcgtgcc gatatagggc gtgaccgcag
     1021 cgaaagagcc ggcggcaaag cctagggcaa tggcatgggg tgtcgcgctg aggcgccgca
     1081 ggcgatagac gacgtagcgc cccgaccggc tccagctgcg gcgcggccaa agatgaatgc
     1141 gcacacgttc cagcaggctc tcggtttgac gacgcttgaa cagcatcaat acctcttggg
     1201 ccaagtttcg gcttgaagaa agccctccta aaggacgtcc ctcaatttag ggtgagttcc
     1261 tctgttcgcc atggttcaag gccagcctgc gacgccgtcc ccctgccgct ctagccgctg
     1321 acccgcacgg cggaagagac gacgtccttg gcgcgcaatc cggcaagaat ctcgttcaaa
     1381 tgcgtgagat cccacacttc gagatcgatg accatctctg catagtccgg aacctgcttc
     1441 atgatcttga tattgtcgat attgccgtcg ccctcgccga tcacggcggc gacttgggcg
     1501 aggctgcccg gctcgttgag cgcggtgacg gcgatcttgg ccgggaagcg ttgcgcattg
     1561 tcctcgtcga tgtcccaggt cacgtcgatc cagcgctccg gctcctcgtc gaacgccttc
     1621 aacgcctcgg catggatcgg atagatggtg atgccttggc cgggggtgag gatgccgacg
     1681 atgcggtcgc cggggacggc gccgtccgct gcgaaactga ccggcagatc gccgcgcaag
     1741 cccctgatcg ggatgctgtt tgcgccagcc gggggctcgc gttcgctgct gctgcttgag
     1801 ccgggccaac ggaacttgag gcccatgacc ttgccgaggc caaaccagcc ttcctcggcg
     1861 cgcttgacct tgcgccggcg cttcggcgcc tcgctcggcg tgtcctcggg ccaggcggcc
     1921 ttgatgacgt cggcggagga cagctcgccg cggccgacgg cggcaatcac gtcttccggg
     1981 gtcttctgcg aaagccgccc gagcgcctga gcgaggacct cctcgctgaa ccgcttgccg
     2041 cgccggctga aggcacgctc gaggatttcg cggccgagct tgccatattg ggcgcgcgcc
     2101 gcatcgcgcg tggcccgcct gatcgccgag cgcgccttgc cggtgacggc gaggctttgc
     2161 caggcggacg gcggcgcctg cacgtcggag gtgatgatct ccacctcgtc gccgttcctg
     2221 agctcggtga tgagcggcat cggccgtcca ttgatcttgc agccgacgca ggtgttgccg
     2281 atgtcggtgt gcaccgcata ggcgaagtcg atggcgttgg cgccgcgcgg gagcgcgatc
     2341 agcctgccct tgggcgtgaa gcagaacacc tgatcctgga acagctcgag cttggtgtgc
     2401 tcgagaaact cttccgggtt gtcgccttcg agcagcatgt cgacgagatg ccgcagccag
     2461 cgataggcgt tgctctcatg actcggcagc acgccgtctt gcgggatcga cccattggcg
     2521 gaatccttgt acagcgcatg cgccgccacg ccatattcgg cgaccgaatg catgcgctcg
     2581 gtgcggagct gcagctcggc gcgctgatga cgcgggccga cgatggtggt gtggatcgac
     2641 tggtagtcgt tctgcttggg gctcgaaatg tagtccttga agcggccggg cacggcatgc
     2701 caggtggtat gcaccacggc gagcgcccgg tagcagtcct caacctcttt caccacgatg
     2761 cggaaggcgt agatgtcgga gagctgctcg agcgaaatct gcttgcgctc catcttgctc
     2821 cagatggcgt agggcttctt ctcgcggaag gcgacgaccg ccggcacctt cgcctcttcc
     2881 agcttcttgc gcagagcgtt ctcgatttcc tcgatcaggc cctgattgcg ctcgcgcagc
     2941 atcttgaggc gatcgacgac ggcggcgtag ccttcagggt tgagccagcg aaaagcgtgg
     3001 tcctcgagct cctcgcgcag ccattgcatg cccatgcgcc cggcgagcgg cgcatagatt
     3061 tccatggtct cctcggcgat gatcctgcgc ttcgcgggct tcatcgcgcc gagcgtgcgc
     3121 atgttgtgta agcggtcggc gaacttgacc aggagcacgc ggatgtcgct cgagatggcg
     3181 agcaacagct tgcggaagtt ttcggcctgt tcggccttct tggtgacgag gtcgagcttc
     3241 ttgatcttgg tgaggccgtc gaccagagag ccgatctcgg ggccgaacag cgcgtcgatc
     3301 tcgctgcgcg tcgcgccggt atcctcgatg gtgtcgtgca agagcgcggt ggcgatggtg
     3361 gcgtcgtcga gccgcatctc ggcgaggatg gctgcgactt ccagcggatg ggagaagaac
     3421 ggagcgcccg aggcccgctt ctgattgcca tgcgccttca tggcgtagac ataggcgcgg
     3481 ttgagcaacg cctcgtcggc gtcgggctcg tagctcttca cccgctcgac cagctcgtac
     3541 tgtctggtca tggcgcgccc ctcatgaagg cgcgacgatg cgacccggca ttgtgggcga
     3601 aggctagatc agcgcgagcg gggtcccgca ttggagccat ctgagggcgc ctgggtcgcc
     3661 atcctgcgca acagctcctc ttcggtcatc ttgtcgatcg cgttgtcgct cgactgatcg
     3721 tcctggccga ggatcggcat gcgagtgtcc gccgagagca tcggcacggc ttctgcttca
     3781 ggctcatcca cttcgacata tttctgcatg gagtggatga agtcttccct aaggtcgtcg
     3841 caggcaatct tctcgtcggc gatctcgcgc agggcgacga ccgggttctt gtcgttgtcg
     3901 cggtccaccg cgatctgggc gccggccgag atggtccggg cgcgatggcc ggaaagcagc
     3961 acaagctcga aacggttggg aatcttgtcg atgcagtcct cgacggtaac gcgagccatc
     4021 agcaaatcct tgtgtcagta agaggaggtt ttcccttatt taggcggttc gcaccggcct
     4081 ggcaaggtaa agcaagggga agagcgcctc aggagaggca tttcagcacg ctgccctgcc
     4141 cctgcttctt cacccgccgc cacagcgcct cggcgcttag cttcaacacc ggatggcgcc
     4201 aattgggggc gacgtcgagc agcggcctga gcacgaacgg ccggagctcg agctgcggat
     4261 ggggcaggac gagcggcctg accctggccc gcggcatggt tttgcggctc tgagaccagt
     4321 tcaggatcat gcccttatag tcgatgatat cgaggtcgag ggtgcgtggg ccccaggggc
     4381 gcccccctcg ccgtccggct tgtgcctcga tctgcttcaa aaggcggagc agggccggcg
     4441 ccggcaggct ggtggcaagc agcgccactt gattgacgta aggtggctgg cgcgccgcgc
     4501 ccatggcagc cgtttcgtag aggttggaca cggcctcgac cgtgatgcct ctccgctcga
     4561 gctcgcgaag cgcccgcttc aaggtcttgg ccgggctgcc ccatggcccg ggagaattgg
     4621 caccaagccc taccaggact tttccgtggg cattcgattg cgcatccacc cttgcattcc
     4681 tatgtttcct atgcagccca gagccgcatt gcctgaaggc cgctcgttcc ttgcatcgat
     4741 attgttaacg ataggactca caatgcattt ctatccaacc gagcgcgtcg ctctcttcat
     4801 cgacggcgcc aatctctacg ccaccgccaa gtctctcggc ttcgacatcg actataagcg
     4861 gctgcttgcg ctgttcggca ccaaggggca gctcatcagg gcgctttact atacggcgct
     4921 cgccgaggag caggagtatt cctcgatcag gccgctcatc gattggctcg actataacgg
     4981 ctacaccatg gtgacgaagc cgaccaagga gttcaccgac gcaaccggcc ggcgcaagat
     5041 caagggcaat atggacatcg agcttgccgt cgacgccatg gagctgtcgg agcacctcga
     5101 ccacatcgtg ctgttctccg gcgacggcga tttccgctcg ctggtcgaag cattgcagca
     5161 caagggcaag agggtcagcg tggtgtcgac gctcgccacc aatccgccga tggtggccga
     5221 cgagttgcga cgccaggccg accagttcat cgaccttgca catatgcaaa gcgagatcgg
     5281 tcgcgatcct gccgaacgcg cccagcgcga gcagcggcgg aggcccgagc cgctcgatga
     5341 attcgattca gaggatctga tcgaaacctg agcggcggag cttggcgagc ccaatgcctc
     5401 ttgcccacaa gacgcgcgcg acaacaccgg aacctgctcc tgattgttcg atctgtccac
     5461 gcctcgtcgc cttccgtgcg accaacagga ccgcccatcc cgactggttc aacggcgcgg
     5521 ttccgtcctt cggccccgac agcgcgcggc tcctaatcgt cg
//
LOCUS       DATNAE010000140         2365 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_1935770, whole genome shotgun sequence.
ACCESSION   DATNAE010000140 DATNAE010000000
VERSION     DATNAE010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1935770"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..722
                     /locus_tag="VN518_03060"
     CDS             171..722
                     /locus_tag="VN518_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HXE03583.1"
                     /translation="MKTLLPASRGYFAIGVEGISKAVNLGNLLRSAHAFGASFVFTLG
                     ADETAIEIRHDTSKAWTHLPVYHWRSLADLRLPKGCSLVGVEILEAAHELPSFPHPLR
                     AAYVLGPERGTLSPELAARCQHLVRIPAAFSLNLATAGAVVMYDRLRSLGRFGSRPVA
                     EHAEAPEPRPHVQGAPKRRRRQG"
     gene            790..1293
                     /locus_tag="VN518_03065"
     CDS             790..1293
                     /locus_tag="VN518_03065"
                     /inference="COORDINATES: protein motif:HMM:NF018482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion associated locus B family protein"
                     /protein_id="HXE03584.1"
                     /translation="MHRAVRAVCLIGALSATPALAQEVKLLDKFKDWSAYAASGPAKV
                     CFAVAKPTESNPKKIKRGPILFYISRWPAENVTSEISVKMGYPFAEGAKVTATIGTAK
                     FDLFTKDEGAFVEKPEMEGKILEAMKGGNSMKIEGKSARGTATSDVYSLNGLSDALDR
                     AAKECSG"
     gene            1395..1691
                     /locus_tag="VN518_03070"
     CDS             1395..1691
                     /locus_tag="VN518_03070"
                     /inference="COORDINATES: protein motif:HMM:NF018151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeA/HdeB family chaperone"
                     /protein_id="HXE03585.1"
                     /translation="MKNLGVAMLAALALLAAKPVSAAEAIDVSKLSCKQFTEYNNDNR
                     GLIMMWFEGYYTEDDEPATIDFGKMAGHLAQILIECEANPDKSVLEVSEEAMDD"
     gene            1795..>2365
                     /locus_tag="VN518_03075"
     CDS             1795..>2365
                     /locus_tag="VN518_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011582176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HXE03586.1"
                     /translation="MSTLSGALAKPTLDAPTHNETLIALSRSELASRLRELGVPERQT
                     RMRVRQLWSWLYVRGATDFAGMTDIAKELRGELAQVFTLARPEIVTEQVSVDGTRKWL
                     LRLADGKEIETVYIPEEDRGTLCISSQVGCTLTCSFCHTGTQRLVRNLTPSEIVGQIM
                     LARDRLGDWPGAAAPDPRGLPDAERKITNV"
BASE COUNT          419 a          778 c          745 g          423 t
ORIGIN      
        1 tttgatcagg acatcgccgg ggccggggct cggctcggcc acctgctcga ggcgaagtgc
       61 ctgaggtccg ccaaattcgc tgaattgcgc aacccgcatc gacgctggcc tcctctcgtt
      121 aacgactggc tgcccttttt ctctcaccca gcttgtggca gacaagactt gtgaaaaccc
      181 tacttcccgc ctcgcgcggc tatttcgcca tcggcgtcga gggcatctcc aaggccgtca
      241 atctcggcaa tctgctgcgc tcggcgcatg ccttcggcgc gagcttcgtg ttcaccctgg
      301 gcgcggacga gaccgccatc gagatccgcc acgatacgtc gaaggcctgg acgcatcttc
      361 ccgtttatca ttggcggagc ctcgccgatc tcagattgcc gaaaggctgt agcctggtcg
      421 gggtggagat tttagaggcg gcgcacgaac tgccgagttt tccacacccg ctgcgcgcgg
      481 cctatgtgct cggcccggag cgcggcacgc tgtctccgga gctggcggcg cgctgccagc
      541 atctggtgcg catccccgcc gccttctcgc tcaatctcgc caccgcgggc gctgtcgtca
      601 tgtacgaccg gctgcgctcg cttggccgct tcggctcacg gcccgtcgcc gagcatgccg
      661 aggcgccgga gccccggccg catgtgcaag gagcgcccaa gcggcggcgc cggcagggtt
      721 gaggggttgt ggtcagggta aaatcgaggc ttaatgcggc tcgcgtgctc acgaatcggg
      781 gacggggaga tgcaccgagc cgttcgagct gtttgcctca tcggcgcgct gtcggccacg
      841 ccagccttgg cgcaagaggt caagcttctg gacaagttca aggactggtc ggcctatgcc
      901 gcgtcgggtc ctgccaaggt ctgtttcgcc gtcgccaagc caaccgaatc caatccgaag
      961 aaaatcaagc gcggtcccat cctcttctac atttcccgct ggcccgcgga gaatgtgacg
     1021 agcgagatca gcgtgaagat gggctatccc ttcgctgaag gggccaaggt caccgccacc
     1081 atcggcaccg ccaagttcga cctgttcacc aaggacgaag gcgccttcgt cgagaagccg
     1141 gagatggagg gcaagatcct cgaggcgatg aagggcggca acagcatgaa gatcgaagga
     1201 aaatccgcgc gcgggaccgc caccagcgac gtctattcgc tgaatggcct gtccgacgcg
     1261 ctcgaccgcg cggccaagga atgcagcggt taagtttctg caaatcctgc cgagcacggt
     1321 ttctttcacc tgacaccgaa ccccactagg ctcgccgccg ccgcggcaag cgccgccaac
     1381 ccttgaggac agccatgaag aatctcggtg tcgctatgct tgccgccttg gcgctgcttg
     1441 ccgcgaagcc cgtttctgcc gccgaagcca tcgatgtgtc caagctcagc tgcaagcagt
     1501 tcacggagta caacaacgac aacaggggcc tcatcatgat gtggttcgag ggctactaca
     1561 ccgaggacga cgagcccgcg acgatcgact tcggcaagat ggcggggcac ctggcgcaga
     1621 tcttgatcga gtgcgaggcc aaccccgaca agagcgtgct ggaggtctcc gaggaggcca
     1681 tggacgatta agtccgcggc ttaccggctt tccgcccgct tcttggcatt tttcgccgcc
     1741 gacatgatat gagggcggtg ccggaatcgg tccggcaggc cgaatgtcat gaccatgtcc
     1801 actctttcgg gcgcgctggc caagccgacc ctggacgcgc ccacgcacaa cgagactttg
     1861 atcgcgctta gccggtcgga gctcgcctcg cgcctgcgcg agcttggcgt gcccgagcgg
     1921 cagacgcgca tgcgcgtgcg ccagctctgg agctggcttt atgtgcgagg cgcgaccgat
     1981 ttcgccggca tgaccgacat cgccaaggag ctgcgcggcg agttggcgca agttttcacg
     2041 ctggcgcgtc ccgagatcgt gacggagcag gtctcggtcg atggcacgcg caaatggctg
     2101 ctgcgtctcg ccgacggcaa ggagatcgag accgtctaca ttcccgagga ggatcgcggc
     2161 acgctctgca tctcgagcca ggtcggctgc acgctcacct gctcgttctg ccataccggc
     2221 acgcaacgcc tggtgcgcaa tctcacgccg agcgagatcg tcggccagat catgctcgcc
     2281 cgcgaccggc tcggcgattg gcccggcgcg gccgcgcccg atccgcgcgg gttgcccgac
     2341 gctgaacgca agatcaccaa tgtcg
//
LOCUS       DATNAE010000141         2082 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2841932, whole genome shotgun sequence.
ACCESSION   DATNAE010000141 DATNAE010000000
VERSION     DATNAE010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2841932"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1583)
                     /locus_tag="VN518_03080"
     CDS             complement(<1..1583)
                     /locus_tag="VN518_03080"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008598567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HXE03587.1"
                     /translation="MRPIVEVAAEKLDIPADYVLPYGHYKAKISLDYIASLAGRPDGK
                     LILVTAITPTPAGEGKTTTTVGLGDALNHIGKKALVCVREPSLGPCFGMKGGAAGGGY
                     AQVVPMEDINLHFTGDFHAIGAANNLLAALIDNHVYWGNKLDIDTRRVTWRRAVDMND
                     RALREIVTALGGAANGFPREAGFDITVASEVMAIFCLAKDLADLQRRLGNIQIGQTRD
                     KKAVKASDLSAAGPMAALLKDALAPNLVQTLENNPAFVHGGPFANIAHGCNSVIATTA
                     ALKLADYVITEAGFGADLGAEKFFDIKCRKAGLKPDCVVIVATIRALKMHGGVAKDDL
                     KKENVAALEKGFANLERHINNLRRYDVPVIVSINRFSTDTEAELALLKRLCATMSVEC
                     VLADHWAQGGAGAEELARTVVRTIETKPSDFHPLYADDMPLWEKARTIAREIYGADDI
                     TADKSVRDRFAELEKEGFGKLPICVAKTQYSFSTNPDAKGAPSGFTIPIREVRLSAGA
                     EFVVVVCGDIMTMPGLPKVP"
     gene            1809..>2082
                     /locus_tag="VN518_03085"
     CDS             1809..>2082
                     /locus_tag="VN518_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HXE03588.1"
                     /translation="MSSVELLKSMFQAAVDAALPSICVPRHLPPKPKGRTVIIGAGKA
                     SGAMAKALEDAWEGPLQGLVVTRYGYRVPMERLEVVEAAHPVPDAAG"
BASE COUNT          341 a          642 c          692 g          407 t
ORIGIN      
        1 ggcaccttgg gcaggcccgg catggtcatg atgtcaccgc agaccaccac gacgaattcg
       61 gcgccggccg aaagccgcac ctcgcggatc gggatggtga agccggacgg cgcacccttg
      121 gcgtcagggt tggtcgagaa gctgtattgc gtcttggcca cgcagatcgg cagcttgccg
      181 aagccttcct tttccagctc ggcgaagcgg tcgcgcacgc tcttgtcggc ggtaatgtcg
      241 tccgcgccat agatctcgcg ggcgatggtc cgcgctttct cccacaacgg catgtcgtcg
      301 gcatagagtg ggtggaagtc gctcggcttc gtctcgatgg tgcgcaccac ggtgcgggcg
      361 agctcttccg ccccggcgcc gccttgcgcc caatgatcgg cgagcacgca ttcgacgctc
      421 atcgtggcgc agaggcgctt gagcagcgca agctcggcct cggtgtcggt cgagaagcgg
      481 ttgatggaaa cgatcaccgg cacgtcgtag cgccggagat tgttgatatg ccgctcgaga
      541 ttggcgaagc ccttctccag cgccgcgacg ttctccttct tgagatcgtc cttggccacg
      601 ccgccatgca tcttgagcgc gcggatggtg gcgacgatga cgacgcaatc aggcttgagc
      661 ccggccttgc ggcatttgat gtcgaagaac ttctccgcgc cgagatcggc gccgaagcca
      721 gcctcggtga tcacatagtc ggcgagcttg agcgcggccg tggtggcgat caccgaattg
      781 cagccatgag cgatattggc gaaggggccg ccatgcacga aggcagggtt gttctccagc
      841 gtctgcacca ggttcggcgc gagcgcatcc ttgagcagcg ccgccatggg gccggcggct
      901 gaaaggtcgc tcgccttaac ggccttcttg tcgcgggtct ggccgatctg gatattgccg
      961 agccgccgct gcagatcggc gagatccttg gcaagacaga agatcgccat gacctcgctc
     1021 gccacggtga tgtcgaagcc agcctcgcgc gggaagccat tagcggcacc gccaagcgcg
     1081 gtgacgatct cgcgcagcgc cctgtcgttc atgtccacgg cgcgccgcca tgtcacccgg
     1141 cgggtgtcga tgtcgagctt gttgccccaa tagacgtgat tgtcgatcag cgccgcgagc
     1201 agattgttgg cggcgccgat ggcatggaaa tcgccggtga aatggaggtt gatgtcctcc
     1261 atgggcacga cctgggcata gccgccgccg gcagcaccgc ccttcatgcc gaagcagggg
     1321 ccaaggctcg gctcgcgcac gcagacgagc gccttcttgc cgatatgatt gagggcatcg
     1381 ccgaggccga ccgtggtggt ggtcttgccc tcgccggcag gcgtcggcgt gatggcggtg
     1441 acgaggatca actttccgtc gggccggccg gcgagcgagg cgatatagtc gagcgagatc
     1501 ttcgccttgt agtggccgta gggcaggaca tagtccgccg gaatgtcgag tttttcggca
     1561 gcgacctcaa cgattggccg catcttggcc gcttgcgaga tttcgatgtc ggattttggc
     1621 ttctggtgct gatcggttgt gcgcgtgccc ggtttctcgg cactttcacg cggctggact
     1681 gacataaatc aatttacccc aggggttaac ggcacgaccg tcgtgcaact cgattgggca
     1741 acctaattca ttcgcttgcc ttgatgcaat cgtctgtgcg aggtcagcgc ccgttgggag
     1801 tccgcgaaat gtcgtcagtc gagctcttaa agtcgatgtt tcaggctgcg gttgacgcgg
     1861 ccctgccctc gatttgcgtg ccccgccatc tgccgcccaa gccgaaaggc cgcaccgtga
     1921 tcatcggcgc cggcaaggcg tccggcgcca tggccaaggc gctcgaggac gcctgggagg
     1981 gtccgctcca gggcctcgtc gtcacgcgtt acggctatcg cgtgccgatg gaacgcctgg
     2041 aagtggttga ggccgctcac ccggtccccg atgctgccgg cc
//
LOCUS       DATNAE010000142         3697 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_1335677, whole genome shotgun sequence.
ACCESSION   DATNAE010000142 DATNAE010000000
VERSION     DATNAE010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1335677"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="VN518_03090"
     CDS             complement(<1..851)
                     /locus_tag="VN518_03090"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXE03589.1"
                     /translation="MKKLTLLGGPALALLLLNALVPGQAARAADTYDINVIMPLTGGG
                     AFLGKAEQQSLQLAEKVVNKSGGIHGRPLRFLFHDDQTSPQTAVQLANQVLAAKPKVL
                     LGSSIVAMCKAMAPLMQNGPVEYCLSPGIHPEKGSYVFTSSVSTFDLANALIRYFRLK
                     GWTRIAIMTSTDASGQDAEHGLGAILGLPENKEMKVVERAHFNTKDVSVTAQLEHVKA
                     ANPQAFIAWSTGAPIATIFRGLVQAGLEVPVATTDGNMTHAQMKQYAAFLPKQLYFPA
                     AEWVVAN"
     gene            complement(920..1420)
                     /locus_tag="VN518_03095"
     CDS             complement(920..1420)
                     /locus_tag="VN518_03095"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HXE03590.1"
                     /translation="MAPVAASGVDVSLELRARLADLYCAYSDALDDGELERWPDFFLE
                     DCLYKVVPRENHERGLPLALIYAESRRMLVDRVVALRETALYAPRRMRHLTGSIRVRA
                     AGPEGLRLTASFMLAQTMVDQPSQLFLCGRYHDRVVESDGGLLFAERICVYDSTIVPT
                     SLVYPV"
     gene            complement(1423..2613)
                     /locus_tag="VN518_03100"
     CDS             complement(1423..2613)
                     /locus_tag="VN518_03100"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HXE03591.1"
                     /translation="MSRVPGWVYADPEIFALEQERIFEGPSWLYVCLEAEIPGPGDFK
                     RSQLGTKQVIAVRDEAGAINVVVNRCAHRGVQFCNANHGTAKEFVCPYHQWTYDLAGK
                     LLGVPFRRGYKGQGGMPGDFTPEAHGLRRLAVARRHGVVFASFVENPEPFETYLGARM
                     LGYFDRVFSGRPLSVLGNMRQRIPSNWKLMFENIKDPYHASLLHVFLVTFGLFRLDQK
                     SEVEMDETGRHAVLVSRKGEQTTNEATAQMRSFKANFALADPRLLDPVREFPGDATVV
                     MQTLWPNLIVQQQSNTLAMRQIVPIDAQSFDLAWTFFGYADDSPEMSRRRLRQANLMG
                     PAGYVSLDDSEAMLLSQAGIGHNPEVPCVVEMGGREVRNAPHMLTETAIRGFYQHYRA
                     VMGL"
     gene            2789..3292
                     /locus_tag="VN518_03105"
     CDS             2789..3292
                     /locus_tag="VN518_03105"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HXE03592.1"
                     /translation="MAKAATSRAREAKGGEFYYPLEHSVGYLVRVTHRSFAQDLQAYL
                     TPHEIPIGMWYFLRALWQEDGLTQRELSQRVGAMEPTTVEQLKNMERRGFVERRRSAA
                     DRRKIHIFMTEEGRALSSRLLPYAMEVNAVALDGLTEGEIGFLRLVLARIKHNLDARQ
                     GQRGLPD"
     gene            3392..>3697
                     /locus_tag="VN518_03110"
     CDS             3392..>3697
                     /locus_tag="VN518_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03593.1"
                     /translation="MAQTARFLDVTGGRPAAMDLWPAIVIPKETIDAEVERLANLPRP
                     ANGRRRALIVHPRATRGNGLAPGIQVALDVLLAGERTVPYRQNSTQVNFVIRGSGHSV
                     "
BASE COUNT          629 a         1214 c         1219 g          635 t
ORIGIN      
        1 tcgttggcga ccacccattc cgccgccggg aaatagagct gcttcggcag aaaggccgca
       61 tactgcttca tctgggcatg cgtcatgttg ccgtcggtcg ttgcgaccgg cacctcgagc
      121 cccgcttgca ccagaccgcg gaatatggtc gcgatcggcg cgcccgtgct ccatgcgatg
      181 aaggcttgcg gattggccgc cttcacatgc tcgagctgtg cggtgacgct cacgtccttg
      241 gtgttgaaat gggcgcgctc gacgaccttc atctccttgt tctcgggcaa gcccagaatg
      301 gcgccgagtc cgtgctcggc atcctggccc gaggcatcgg tgctcgtcat gatcgcgatg
      361 cgcgtccagc ccttcaggcg gaaatagcga atgagcgcgt tggcgagatc gaaggtcgac
      421 acgctcgagg tgaagacgta gctgcccttc tccggatgaa tgcccggcga gaggcaatac
      481 tcgacgggac cgttctgcat cagcggcgcc atcgccttgc acattgccac gatcgaggag
      541 ccgagcagca ccttcggctt ggctgcgagc acctggttcg cgagctgcac cgccgtctgc
      601 gggctcgtct gatcgtcgtg gaagaggaag cgaagcggcc ggccgtggat gccgccgctc
      661 ttgttcacga ccttctccgc gagttgcagc gactgctgct cggccttgcc gaggaatgca
      721 ccgccgccgg tcagcggcat gatgacattg atatcgtagg tatcggcggc gcgagccgct
      781 tgtcccggaa ccaatgcatt cagcagcagc agggcgagcg ccgggccgcc gagcagcgtc
      841 aatttcttca tcgcttcacc tcccttgcgc ggtcggccga aaccgccgat cgcttatttt
      901 tgccgcctcc ccggcaagcc tagaccggat aaaccagcga ggtcggcacg atggtcgaat
      961 cgtagacgca gatcctctcg gcgaagagca ggcctccatc gctctcgacg acgcggtcat
     1021 ggtacctgcc gcagaggaag agctgcgatg gctggtccac catcgtttgc gccagcatga
     1081 agctggcggt aagccgcagg ccctccgggc cggccgcgcg cactcgtatg ctcccggtca
     1141 ggtgacgcat cctgcgcggc gcgtagagcg cggtctcgcg cagcgccacc acgcggtcga
     1201 cgagcatgcg ccggctttcg gcatagatca gggcgagcgg cagcccgcgc tcgtggttct
     1261 cgcggggaac gaccttgtag agacagtcct cgaggaagaa atcgggccag cgttcgagct
     1321 cgccatcgtc gagcgcgtcg gaataggcgc aatagaggtc ggcgagccgc gctctcaatt
     1381 cgagggacac atcgacgccg gaagcggcga ccggggccat gcctaaagcc ccattaccgc
     1441 gcgataatgc tggtagaagc cgcgaatcgc ggtctcggtc agcatatgcg gcgcgttcct
     1501 cacctcgcgc ccgcccatct cgacgacgca gggcacctcg gggttgtgcc cgatgccggc
     1561 ctgcgagagc agcatcgcct cgctatcgtc gagcgagaca tagccggccg ggcccatgag
     1621 gttggcttgg cgcaggcggc gccgcgacat ctcgggcgaa tcgtcggcat agccgaagaa
     1681 ggtccaggcg agatcgaagc tctgcgcatc gatcgggacg atctggcgca tcgccagcgt
     1741 gttcgactgc tgctgaacga tcaagttcgg ccagagcgtc tgcatgacca ccgtggcatc
     1801 gccgggaaat tcccggaccg ggtcgagcag gcgcggatcg gcgagcgcga aattcgcctt
     1861 gaacgagcgc atctgtgcgg tcgcctcgtt cgtcgtctgc tcgcccttgc ggctcaccag
     1921 caccgcgtgc cggccggtct cgtccatctc gacctcggat ttctggtcga ggcgaaaaag
     1981 accgaaggtg acgagaaaga cgtgcagcaa gcttgcgtga tacggatcct tgatgttctc
     2041 gaacatcagc ttccagttgc tcggaatccg ctgccgcatg ttgccgagca ccgagagcgg
     2101 tcggccggag aagacgcggt cgaaatagcc gagcatccgc gcgccgaggt aggtttcgaa
     2161 gggctcggga ttctcgacga agctcgcgaa gacgacgccg tgccgccggg cgaccgcgag
     2221 gcggcgcagg ccatgggctt ccggggtgaa gtcgcccggc attccgccct gccccttgta
     2281 gccgcgccgg aacggcacgc cgaggagctt gccggcgagg tcataggtcc attggtgata
     2341 gggacagacg aattccttgg cggtgccatg gttggcgttg cagaactgaa cgccgcggtg
     2401 agcgcagcga ttgacgacga cgttgatcgc gccggcctcg tcccgcaccg cgatcacctg
     2461 cttggtgccg agctggctgc gcttgaagtc gccgggcccg gggatttccg cttccaggca
     2521 gacgtagagc cagctcgggc cttcgaagat gcgctcctgc tcgagcgcga aaatctccgg
     2581 gtcggcgtag acccatcccg ggacgcgcga caccccttct tccggccatc tcagcgcggc
     2641 gcccgccgac gacggcgttc ctcccccgtt tgctgacggc atccggcccc tcgacaagca
     2701 cgcagctcag ccatatggtt agggacctta gtttatgggt cggcgggagg caaccccttt
     2761 cggccgagcg cgcggagatc ggagcacgat ggcaaaggcg gcgacgtccc gcgctcgcga
     2821 ggcgaagggg ggcgagttct actacccgct cgagcacagc gtcggctatt tggtgcgcgt
     2881 cacccatcgc agcttcgccc aggatctcca ggcgtatctg acgccgcacg agatcccgat
     2941 cggcatgtgg tatttcctgc gtgcgctgtg gcaggaggat ggcctcaccc agcgcgagct
     3001 gagccagcgc gtcggcgcga tggagccgac aaccgtcgag cagctcaaga acatggagcg
     3061 ccgcggcttc gtcgagcggc ggcgcagcgc ggccgatcgc cgcaagatcc atatcttcat
     3121 gaccgaggag ggccgcgccc tgtcctcgcg cctgctgccc tacgcgatgg aggtgaacgc
     3181 ggtcgcgctc gacgggctga ccgagggcga gatcggcttt ctccgcctcg tgctcgcccg
     3241 catcaagcac aatctcgatg ctcggcaggg ccaacgcggc ttgcccgatt gacaggcggc
     3301 gcggcacggc gattaaatcg cttcgcttcc taagggaatt ttcggcgccg gatcgcggcg
     3361 cacgtaccgc cgtccagctg aggagctcaa catggcgcag accgcacgct ttctcgacgt
     3421 gaccggcggc aggcccgccg cgatggacct ctggcccgcg atcgtcattc cgaaggagac
     3481 gatcgatgcc gaggtcgagc ggctcgccaa tctgccgcgt ccggccaatg gccgtcgccg
     3541 cgcgctcatc gtgcatccac gcgcgacgcg cggcaacggc cttgcgcccg gcatccaggt
     3601 cgcgctcgac gtgttgctgg ccggcgagcg caccgtcccc taccgccaga attcgacgca
     3661 ggtcaatttc gtgatccgcg gctcgggtca ctcggtg
//
LOCUS       DATNAE010000143         2221 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2845832, whole genome shotgun sequence.
ACCESSION   DATNAE010000143 DATNAE010000000
VERSION     DATNAE010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2845832"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..1203
                     /locus_tag="VN518_03115"
     CDS             91..1203
                     /locus_tag="VN518_03115"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HXE03594.1"
                     /translation="MTVAARDARIIGAEGRSYVNFASNDYLGLRFHPALIARAVEWAE
                     TYGVGSGASRLVTGNLDLFAPIETKLATLKGKPASLVMASGFQTNAAVLQALFDPSVL
                     GAEPLVFADRLNHASMHFGCQAAGVRQLRYRHCDVAHLAELLSQYQGDERPRFILTES
                     VFSMDGDVAPLEEIARLAREHDACLVVDDAHATGILGRAGSGLSAGADIVIGTFSKAF
                     GGFGGYVASTETMRDYLINRCGGIIYSTALPPPVLGAIDAALDLVSGMDEERTHVARL
                     AERFRAGASAIGLDTGASATQIVPVILGSPQAAIAMSERLKRAGLWASAIRPPTVPTG
                     TARLRLAFTAAHEDSDIDRLLDVLAKEDARRPASAM"
     gene            1213..1839
                     /locus_tag="VN518_03120"
     CDS             1213..1839
                     /locus_tag="VN518_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03595.1"
                     /translation="MRFVLVHGWGFHAGIFADLISHLDGAETTLIDLGFVSGGPKGET
                     DWPADAIAIGHSLGLLWLLEQGGGRFRGLVSIQGFDCFCCHIVPSRIAALKRGLERDP
                     GATLQAFWRSCGAAGFALPEALNVARLDQGLDWLMHWDARKAKQELACPVLALAARDD
                     AVVPASMSEAIWEDTGIIWSQDGGHVLPLRHPRWCARHVLEFANALPS"
     gene            1823..>2221
                     /locus_tag="VN518_03125"
     CDS             1823..>2221
                     /locus_tag="VN518_03125"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXE03596.1"
                     /translation="MLSRRSAEIAASFGARAKSYERHAGLQRAVADRLARLLPERKQP
                     NVLELGCGTGLFSRHLLARYPDGRFVLTDAAPAMIAECRRNLAAADQARISFEVMDAG
                     EAGGHAELDLIVSSMTLHWLPDPIASLDRLR"
BASE COUNT          332 a          758 c          723 g          408 t
ORIGIN      
        1 cttcgctaat acaggatcat ggcggcaagc gaaagcaggt acagggcctt tctcgacgct
       61 cgcgctcgcg agggcttgcg gcgatcgttc ttgacggtcg cggcccgcga tgcgcggatc
      121 atcggcgcgg aagggcgctc ctacgtcaat ttcgccagca acgattatct cgggcttcgc
      181 ttccacccgg cgctgatcgc ccgtgcggtc gaatgggcgg aaacctacgg cgtcggctcc
      241 ggagcctcgc ggctcgtcac cggcaacctc gacctgtttg cgccgatcga gaccaagctt
      301 gccacgctca agggaaaacc ggcgtcgctg gtgatggcgt cgggcttcca gaccaacgcc
      361 gcggtgcttc aggcgctgtt cgacccgagc gtgctcggtg ccgagcctct cgtcttcgcc
      421 gaccggctca atcacgcgag catgcatttc ggctgccagg ccgccggagt gcggcagctc
      481 cgctaccggc attgcgacgt cgcccatctc gccgagcttc tgagtcaata tcaaggagat
      541 gagcggccgc gcttcatcct gaccgagagc gtgttctcca tggatggcga cgtcgcgccg
      601 ctcgaagaga tcgctcgcct cgcgcgcgag catgacgcct gcctcgtcgt cgacgacgcg
      661 catgccacgg gaattctggg acgcgcgggc tccggtctct ccgccggcgc cgatatcgtc
      721 atcggcacct tctccaaggc gtttggcggc tttggcggct atgtcgcttc gaccgagacg
      781 atgcgcgact atctcatcaa ccgctgtggc ggcatcatct actcgacagc gcttccgccc
      841 cccgtgctcg gcgccatcga tgccgcgctc gacctcgtgt ccggcatgga tgaagaacgg
      901 actcatgtcg cccgcttggc cgagcggttc cgcgcgggtg cgagcgccat cgggctcgat
      961 accggcgcct cggccacgca aatcgtgccg gtcatcctcg gatcgccgca agctgccatc
     1021 gccatgagcg agcgcctcaa gcgggccggc ctgtgggctt cggccatccg gccgccgacg
     1081 gtgcctaccg gcacggccag gctccggttg gccttcaccg cggcgcatga agactctgac
     1141 atcgaccggc tcctggatgt ccttgccaaa gaggatgcgc ggcgccccgc gagcgccatg
     1201 taaagtcgca agatgcgctt cgtcctcgtt catggctggg gtttccacgc cggcatcttt
     1261 gccgatctca tcagccatct cgacggcgcc gagaccacgc tgatcgatct cggcttcgtg
     1321 tccggaggac cgaagggcga gacggactgg ccggcggacg ccattgccat cggccattcg
     1381 ctgggtttgc tatggttgct ggagcagggg ggtggccgct tcaggggtct cgtcagcatc
     1441 cagggcttcg actgcttttg ctgccatatc gtcccctcgc ggatcgccgc gctgaagcgc
     1501 gggctcgagc gggaccctgg agcaacgctc caggccttct ggcggtcttg cggcgcggcg
     1561 ggcttcgctc taccggaagc cctcaacgtc gcgcggctcg accaagggct cgattggttg
     1621 atgcattggg atgcgcggaa ggctaaacag gagcttgcct gcccggtcct cgcgcttgcc
     1681 gcccgcgacg acgccgtcgt tccggcttcg atgagcgagg cgatctggga ggacaccggc
     1741 atcatctggt cacaggacgg gggccatgtc ctgcccctga ggcacccacg ctggtgcgcg
     1801 cgtcatgtcc tcgaattcgc caatgctctc ccgtcgtagc gcggaaatcg ccgcaagctt
     1861 cggcgctcgc gccaaaagct acgagcgtca tgccgggctt cagcgcgcgg tcgccgaccg
     1921 gctggcgcgc ctcctgcccg agcgcaagca gccgaacgtg ctcgagctgg gatgcggcac
     1981 cggcctgttc agccgccatc tgcttgcgcg ctacccggac ggacgcttcg tgctgaccga
     2041 cgcggcgccc gccatgattg ccgaatgccg gcgcaatctc gcggctgcgg atcaggcgcg
     2101 catcagtttc gaggtgatgg atgcgggcga ggctggaggc catgccgaac tcgacctgat
     2161 cgtgtcgagc atgacgctgc attggcttcc cgaccctatc gcgagcctcg atcgcttgcg
     2221 g
//
LOCUS       DATNAE010000144         2407 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3745618, whole genome shotgun sequence.
ACCESSION   DATNAE010000144 DATNAE010000000
VERSION     DATNAE010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3745618"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1366)
                     /locus_tag="VN518_03130"
     CDS             complement(<1..1366)
                     /locus_tag="VN518_03130"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HXE03597.1"
                     /translation="MSRRWRGLMGAGPILALLASVLSAGAEAEEKAKADVVLRNGKIY
                     TADPARSVRQALAFTGNTIVAVGSDTDVAPLIGPKTKVVDLVGKLVLPGLIDTHIHPI
                     IGALNGAKCGLAGVKATIDTLKPVIQDCLAKDQGSADEWFEAAQLDNYGFSATAKDLD
                     TIEATRPVALWGNDGHTVWVNSRGLALLGVTAETPDIPGGKIARDGNGTPTGYFADRA
                     SIFVDEKIPSPPLEEKVALTAAELKRMSAYGITSLMDAFVTPAEEAVWRKLYDPGRLP
                     MRVRTAIYVADPSDDSDDAVARLLAASKQGDVDTEILRAGAVKVFADGVMEYPAQTAA
                     LLKPYLDANGKPTKHEGELYFDPERFARLVQKLDAAGLTVHVHAIGDRAVRASLDAFA
                     SARAANGDKDNRHQIAHLQLVDPADFPRFKELGVIADFQLEWAKREPATEGPLEPYLG
                     PERYR"
     gene            complement(1389..2111)
                     /locus_tag="VN518_03135"
     CDS             complement(1389..2111)
                     /locus_tag="VN518_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HXE03598.1"
                     /translation="MPAFLAGLAALLILVFGARVLASANPQKLAASMRMAGGILLLLV
                     AAFLLARGAFPLAIPVAFFGLALLGLPVRSWFGSGGPFASRKSPGRKSEVRTEGLEMV
                     LDHDSGEMEGRCLKGQFAGRMLSSLSEAELLVLLAELRSTDAQGAALIEAYLDRRAQG
                     WREGRAEEAAKEQARAPRGGRIRMPREEAYEVLGLKKGAGEDEIRAAHRKLMMKVHPD
                     QGGSDYLAARINEAKDVLLGRK"
     gene            complement(2125..>2407)
                     /locus_tag="VN518_03140"
     CDS             complement(2125..>2407)
                     /locus_tag="VN518_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXE03599.1"
                     /translation="IDELCGRAGELALLGVPMFLFQEGREPRAERAFKEIARLTRGAY
                     CHFDAGSARQLRELLAAVAVFATGGRKALQDYGAATKSGAAVRLLEQLS"
BASE COUNT          405 a          808 c          775 g          419 t
ORIGIN      
        1 agcgatagcg ctcaggccca agataaggct cgagtgggcc ttcggtcgcc ggctcgcgct
       61 tggcccattc gagctggaaa tcggcgatca cgccgagctc cttgaagcgc gggaagtctg
      121 ccggatccac gagctgcaga tgagcgatct ggtgacggtt gtccttgtcg ccgttcgccg
      181 cgcgggctga agcaaatgcg tcgaggctcg cacgcacggc gcggtcgccg atggcgtgga
      241 cgtggacggt aagaccggct gcgtcgagct tctggaccaa gcgcgcgaaa cgctctggat
      301 cgaaataaag ctcaccttca tgctttgtcg gcttgccgtt cgcgtcgaga tagggcttta
      361 gcagcgccgc cgtctgcgcc gggtattcca tgaccccgtc ggcgaagacc ttgaccgcac
      421 cggcgcggag gatttcggta tcgacgtcgc cttgcttgct tgccgcgagg agacgcgcga
      481 cggcgtcgtc gctgtcgtca ctcggatcgg caacgtagat cgctgtgcgc acgcgcatgg
      541 ggagcctgcc ggggtcgtac agcttccgcc aaacggcctc ctctgccggc gtcacaaagg
      601 catccatcag cgaggtgatg ccataggccg acatcctctt cagttcggcc gcggtcagtg
      661 ccaccttttc ctccagcggc ggcgacggaa tcttctcatc cacaaagatc gaggcgcggt
      721 cggcgaagta gccggtcggc gtgccgttgc cgtcgcgggc gatcttgcct cccggtatgt
      781 ccggcgtttc ggcggtcacc ccgagcaatg caaggccgcg gctgttgacc cagaccgtgt
      841 gcccgtcatt gccccagagc gcgacaggcc tcgtcgcctc gatggtgtcg aggtcctttg
      901 ccgtcgccga gaaaccgtaa ttgtcgagct gcgcagcctc gaaccattcg tcggcactgc
      961 cctggtcctt ggcgaggcaa tcctggatga ccggcttcag tgtgtcgatc gttgccttca
     1021 ccccggcgag gccgcatttg gcaccgttga gcgcgccgat gatgggatgg atgtgggtat
     1081 cgatcaggcc gggcagcacg agcttgccga caaggtcgac gaccttcgtc tttggcccga
     1141 tcagcggcgc cacatcggtg tcggaaccga cggcgacaat ggtattgccg gtgaaggcaa
     1201 gcgcctgccg gaccgagcgt gcggggtccg ccgtatagat cttgccgttg cgcagaacga
     1261 catcggcctt ggctttctcc tctgcctcgg cgccggcgga caagaccgac gcaagcagag
     1321 caagaatcgg ccccgccccc atcaaacccc tccaacgtcg cgacaatcca cgcctccctt
     1381 gttccggctt acttgcgccc gagcaggacg tccttggcct cgttgatccg cgccgcaagg
     1441 tagtccgaac cgccttggtc ggggtggact ttcatcatca gcttgcgatg cgccgcgcgg
     1501 atctcgtcct cgcccgcccc cttctttagc ccgagcacct cgtaggcctc ttcacgcggc
     1561 atgcggattc gtccgccgcg cggagccctc gcctgctcct tggcagcctc ctccgcccgt
     1621 ccttcgcgcc agccttgcgc gcgccggtcg agataggcct cgatcagggc agcaccttga
     1681 gcgtccgttg agcgcagctc cgcgagcaga acgaggagct cggcttcgct caacgaggac
     1741 agcatcctcc ctgcgaactg gcctttcagg cagcgcccct ccatctcgcc gctgtcatga
     1801 tcgagcacca tttcgagccc ttcggtgcgc acctctgatt tccgccccgg cgacttgcgc
     1861 gacgcaaacg gtccgccgga gccgaaccag gatctgaccg gcagcccgag cagagcgagg
     1921 ccgaaaaagg caacaggaat ggcaagcggg aaggccccgc gggcgagcag gaaggcggcg
     1981 accaagagaa gcaggatgcc tcctgccatg cgcatgctgg cggcaagctt ctgaggattc
     2041 gccgacgcga gcacgcgggc gccgaaaacg agaatgagga gagccgctag gccggcgagg
     2101 aaggctggca ttgtctaaag agcgctacga gagttgttcg agaagccgca ccgcggcgcc
     2161 gctcttggtc gctgcgccgt aatcttgcag ggccttgcgc ccgccggtgg cgaagacggc
     2221 gaccgccgcc aacagctcgc gcagctgccg ggccgagccg gcgtcgaaat gacaataggc
     2281 gccgcgcgtg agcctcgcga tctccttgaa ggcgcgctcg gcccgtggtt cccgcccctc
     2341 ctggaacagg aacatcggca cgccgagcag cgcgagctcc ccggcgcggc cgcaaagctc
     2401 gtcgatg
//
LOCUS       DATNAE010000145         6565 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3447084, whole genome shotgun sequence.
ACCESSION   DATNAE010000145 DATNAE010000000
VERSION     DATNAE010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6565
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3447084"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            305..775
                     /locus_tag="VN518_03145"
     CDS             305..775
                     /locus_tag="VN518_03145"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HXE03600.1"
                     /translation="MTESSKAAAEKARDTYRKAAAQFEDLAGDAQVPEAMRALAEKNI
                     AQTREIYERSKDALDTVLESWERSFDAVGQGAVALNRKVIDIAQRNINSGFDLAKRLA
                     AAKNLAEAMELHATYWRNQISALQSQAEEMRTLSTKVTADAAEPIKTQMTRGLD"
     gene            1044..2009
                     /locus_tag="VN518_03150"
     CDS             1044..2009
                     /locus_tag="VN518_03150"
                     /inference="COORDINATES: protein motif:HMM:NF041770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CFI-box-CTERM domain-containing protein"
                     /protein_id="HXE03601.1"
                     /translation="MLLNTRRRMLGLLAAAIGTSRLVQLIPPALAADTEEKPASKKSE
                     CFALQAFGDWKGTATNKQAGARIGQVTFSDPDACDLRAEIQVAASYDAKLVIFGDPDG
                     TPLPKDFLVKPDNRIIAKNEDGTTAVDEPLCGVCTDIRDDKVSIVLPLSTGALFRSAK
                     SVEMAVKLGDAEQCSFTLNCEDLRKALDWAVQRKDELAKSFDEKKCTPPAKGCFLTTA
                     CCEVLGLSDDCFELRTLRRYRDRVLIAMPGGQAALAAYYLVAPSILERLPQQERAARL
                     LSIYMRFILPSAVAARLGLNPLAYSLYARMMDELSRSDGALPAGR"
     gene            2316..3422
                     /locus_tag="VN518_03155"
     CDS             2316..3422
                     /locus_tag="VN518_03155"
                     /inference="COORDINATES: protein motif:HMM:NF028178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HXE03602.1"
                     /translation="MAARILRIRLPLVFVAIIGMVASGDATLGTGKDLDPNSSADWLG
                     VIAGDEAGSEIELAAEMAELLANASPFRVVPVPGDSGLNNISRLLHDPHIDAGFVSTD
                     VLADTDTQSSMPNLAGSLQVVARLCPQEVHVLARAGIDSLADLAGKKVNFGPDGGSSA
                     VTARTLFRALNIEVEPVALDSRAAVEQLTQGAIAASVFVGAKPMPLIAGIPANHGLHL
                     LPVAFGAGLEAVYLPTGFDHDDYPNLIEMETEIPSVATGMALLAAASKDDPGHADRVA
                     QLVDTLFSHFGELKKDGRHPKWREINLAASLPGWTRAPAAEAWLAQHPNEDARKGTQA
                     KIEAGAAQSNLPVRQNQRDVLFEQYIEWQRAKGQ"
     gene            3509..4855
                     /locus_tag="VN518_03160"
     CDS             3509..4855
                     /locus_tag="VN518_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03603.1"
                     /translation="MIGSAAPCRAEPGLQPLRQLLPRAYLEADRPAAIPGVRWDAIHR
                     STFPYIETPQYTRRIARRMALPRSLAARLRTLVPDDEELGTFLEDLALAVRAETGSGY
                     DLSDSEAFEAGLPLEPKPAPQAHMQALVHDFRRRQRQASMLVAGCVATSIVLTVVGIA
                     ALASFAKPSPADADPLVKSSSSVVWHGSQDSALPKLILAKAAPFTEPAPVEEEASSPP
                     PEAVTTSSSAANTAPQLIMVQAGRPLELAPLLTQRQARYILVRGLPSEAKLSAGQRNP
                     SGAWLVKERDADHLTLSIGGAASGDYPVEVYALGAASAPQARQRLVFRVAAGPTLASA
                     AGASGTLFNAAQAPAPAQPAAAAPASPLMARAMRLLGEGDIAGARLLLMHLAEQGEAD
                     AAYELARTFDAEYLAELGARGVGPDKTRAVGWYEWASESGSVKAAERLKILASLSD"
     gene            complement(4925..>6565)
                     /gene="cysC"
                     /locus_tag="VN518_03165"
     CDS             complement(4925..>6565)
                     /gene="cysC"
                     /locus_tag="VN518_03165"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008190436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HXE03604.1"
                     /translation="EQKITIDVAYRYFSTPRRKFIVADTPGHVQYTRNMATGASNCDF
                     AVLLVDARSGVLTQTRRHACIVSLLGIRKLALAVNKMDLVDFTQARFDAIAEDFRSFA
                     EPLGFEAVTCIPVSSLKGDNVIVPSPHMHWYRGPTLLGHLETVDVSREEKEEPFRFPV
                     QWVNRPHLDFRGYAGTIASGKVRPGDEVMIQPSGKRVHVARIVTADGDLEVAHRGDAI
                     TLTFAEEVDASRGDIVCGIEEAPAIADQIAAHIVWFDEEPMLPGRSYALKCGCQTTTA
                     TISSLKYKLNVDNLEHVAGKTLELNEVGSCNLSTGKALVFDPYAENSETGSFILIDRF
                     TYGTVAAGMIEFGLRRATNIQWQALSIDKQARSRLKGQQPCVLWFTGLSGSGKSTIAN
                     MLEKRLHALGRHTYVLDGDNIRHGLNKDLGFTDADRVENIRRVAETAKLFVEAGLIVM
                     VSFISPFRSERRMARELLQEGEFVEVYVDTPLEICEARDPKGLYRKARAGLISHFTGI
                     DSDYEVPEAAELTLDTSKTTPDALAEALLAELRRRHVT"
BASE COUNT         1185 a         2127 c         2055 g         1198 t
ORIGIN      
        1 caaagcgcct cacatcaaag tctgcacgcg cggcgcaaaa tgtgctccgc gagggggttt
       61 acggtcttcg cctcgcgcgc cgccagcgat cacgggtaag aaccgccgag ttcgaagcgc
      121 ttaacccata gtgttgagaa ctcggtttgc ggggcgcgaa gcgcgtttgc gcgccacgct
      181 tgcaaagtct ctcgccaagg tcttaccctg cccaatggcg gcccggcctt tgcgcatgga
      241 agcgtgccgg ctcgggtcgt ccagcccctg ctaaccttct gcgtaacccc aagaggccag
      301 taccatgacc gaaagttcaa aagctgccgc cgagaaggcg cgcgacacgt atcgcaaggc
      361 cgctgctcaa ttcgaggact tagccggcga cgctcaggtg ccggaggcaa tgcgcgcttt
      421 ggccgaaaag aacatcgctc aaactcgcga gatctatgag cgctccaagg acgcgctcga
      481 cacggtgctc gagagctggg aaaggtcgtt cgacgcggtc ggtcagggtg ccgtcgcgct
      541 caaccgtaag gtcatcgaca tcgcccagcg caacatcaat tccggcttcg acctcgccaa
      601 gagattggcg gcggcaaaga acctcgccga agccatggag ctgcacgcga cctactggcg
      661 caaccagatc agtgccttgc aatcccaagc cgaagagatg cgcacgctct cgacgaaggt
      721 gaccgccgac gcggccgaac cgatcaagac acagatgacg cgcggcctag attgagcaag
      781 gccgccagct gacccgtttg gtgtctgtga ggttgcccca gggtgactct gtctgagccg
      841 cgcacttagc ggaatttgcg gcgacgatcg ggtattgccg caactccaag cttcgaagcc
      901 ccgggatgcc tcttctcggg gcttcgtctt gcctaatacc ctcacccgac ggccggtttg
      961 acaagccgcc gttctcgtga aacaggcatt gaccgttttc cgcggggcgt gcgcctcgcg
     1021 cgccagtttc tcgggatcgg cccatgcttc tgaatacgcg ccgcaggatg ctcggactgc
     1081 tcgccgccgc gatcgggacc tcgcgcctcg ttcaactcat cccgccagcc ctcgcagccg
     1141 acaccgagga gaagccggcc tccaagaagt ccgagtgctt cgcgctgcag gccttcggcg
     1201 attggaaagg gacggcgacc aacaagcaag ccggcgcccg catcggccag gtgacctttt
     1261 ccgatcccga cgcttgcgat ctccgcgccg agattcaagt ggcggcgagc tacgatgcga
     1321 agctcgtgat cttcggcgat ccggacggca cgccgctgcc gaaagacttt ctcgtcaagc
     1381 ccgacaatcg catcatcgcc aagaacgagg acggtaccac tgccgtcgac gagccactct
     1441 gcggggtgtg caccgacatc cgcgacgaca aggtgagcat cgtcctgccg ctatcgaccg
     1501 gagcgctgtt ccgctcggcc aagtcggtgg agatggcggt gaagcttggc gacgccgagc
     1561 aatgcagctt cacgctcaat tgcgaggatt tgcgcaaagc tctcgactgg gcggtgcagc
     1621 gcaaggacga gctggcgaaa tccttcgacg aaaagaagtg cacgccgccg gccaaaggct
     1681 gcttcctgac caccgcctgt tgcgaggtgc ttggcttgag cgacgattgc ttcgagctcc
     1741 gcacccttcg ccgctatcgc gaccgcgtgc tcattgccat gccgggcggt caagccgccc
     1801 tcgccgccta ttatcttgtg gcgccgtcga tactggagcg gcttccgcaa caggagcggg
     1861 cggccagact cttgtcgatc tacatgcgct tcattctgcc gagcgccgtg gcggcgcggc
     1921 ttggcttaaa ccctctcgct tattctcttt atgcgcgcat gatggatgag ctttcgcgca
     1981 gcgacggagc tttacccgcc ggccgatagg ctaccctcgc gcgtcgcgct tcctgttacc
     2041 tcgccgacac ttgtcaagca ccaagcctcg gcttcgccgc aatcaggacg aagtggcgcc
     2101 ccagtcgcga gttacgctca tgtttaacaa aaagtgaacg ttttgccccg aacggggttt
     2161 gagacacggg aagaatgccg gacaggagtt tagctgccgg ccttcgagat gcaggggggg
     2221 tgtcgatttc atgcgttgcc cgtccacatg ctcgacggtt gaggcgcccg gcgctctaga
     2281 agctccggct cctgccgtga agagggacgg gtgtcatggc ggcgcgcatt ttgcgtatcc
     2341 gactgccgtt ggtttttgtc gccattatcg gcatggtcgc ctcgggcgac gcaaccctgg
     2401 gaaccggcaa ggacctcgat cccaattcaa gcgcagactg gctcggtgtg atcgcgggcg
     2461 acgaggccgg atcggaaatc gagcttgctg ccgagatggc tgagctcctc gccaacgcca
     2521 gcccattccg cgtcgttccg gtaccgggcg acagcggcct caacaacatt tcacggcttt
     2581 tgcatgaccc gcacattgat gcgggttttg tttcgaccga cgtgctcgcc gataccgaca
     2641 cgcagagctc catgccaaat ctcgccggga gcctacaggt ggtggctagg ctctgtccgc
     2701 aggaggttca tgtcctggcg cgggccggca tcgacagcct cgccgatctc gccggcaaga
     2761 aagtgaattt cggccctgac ggcggaagct cggcggtcac tgccaggact ctgttccggg
     2821 ctctgaacat cgaggtcgag ccggttgctc tcgatagccg cgctgcggtc gagcaactga
     2881 cgcaaggtgc gatcgccgcg agcgtattcg tcggcgccaa gccgatgccc ctgatcgccg
     2941 ggatcccggc aaatcacggc ctgcatctcc tccctgtcgc attcggcgca ggcctcgaag
     3001 ccgtttattt gccgacgggc ttcgaccacg acgattatcc aaacctgatc gaaatggaga
     3061 ccgagattcc gtcggtcgcg accggcatgg cgctcctcgc ggcggcctcc aaggacgatc
     3121 caggtcacgc cgatcgtgtg gcgcaattgg tcgacacgct cttctcgcat ttcggcgaac
     3181 ttaagaaaga cggcaggcat ccgaaatggc gcgagatcaa tctcgccgca agcctcccgg
     3241 gctggacccg ggcgccagcg gctgaagcat ggttggcaca gcatccgaat gaagacgcgc
     3301 gcaaaggcac acaggcaaag atcgaggctg gcgcagctca gtcaaatctg cccgtgaggc
     3361 aaaatcagag ggacgtattg ttcgaacagt atatcgagtg gcagcgcgct aaagggcaat
     3421 agcgcgcttt tttttgtggg agggggaaga cggggagcgt aacgccgggc ggccaagccg
     3481 ctcggcggag taaccatgga gtacaaatat gatcggttcc gccgcgccct gcagagcaga
     3541 gcctggtttg caaccgttgc gtcaactgct gccccgcgcg tacctcgaag cggatcgtcc
     3601 ggcggcaatt cccggggtgc gctgggacgc aatccaccgc agcacctttc cgtatatcga
     3661 gacgccgcaa tacacgaggc gcattgcccg ccgcatggca cttccccgct cgctcgcggc
     3721 tcgactgcgg accttggtgc ctgacgacga ggagctcggc acctttcttg aggaccttgc
     3781 gctcgcagtc cgcgccgaga ccggcagcgg ctacgatctc agcgactctg aggcctttga
     3841 ggcgggcttg ccgctcgaac cgaagcctgc cccgcaagcg catatgcagg cgctcgttca
     3901 cgacttccgc cgtcgtcaac gccaagcgag catgctcgtc gccgggtgcg ttgccacgtc
     3961 gatcgtgttg accgtggtcg gcattgcggc ccttgcaagt ttcgccaagc cctcccctgc
     4021 cgacgccgac cctctggtca aaagctcgag ttccgtggtt tggcacgggt cgcaagactc
     4081 cgcgctccca aagctcatcc tcgccaaggc ggcgcccttc accgagcctg cgccggtcga
     4141 ggaagaggct tcgagccctc cgccggaggc cgtgacaacc tcgtcgtcgg cggccaatac
     4201 ggcgccgcaa ttgatcatgg tgcaagccgg ccgcccgctc gagcttgctc ccctgctcac
     4261 ccagcggcag gcgcgctaca tcctcgtccg cggccttccc agcgaagcca agctctccgc
     4321 cggccagcgc aacccgagcg gtgcctggct cgtgaaggag agggacgccg accacctgac
     4381 cttgtcgatc ggtggcgccg cgagcggcga ctatcccgtg gaagtttacg ctttaggcgc
     4441 cgctagcgcc ccccaggccc ggcagcgttt agtgttccgc gtcgctgccg ggcctacctt
     4501 ggcgtcggcc gccggggcct ctggaactct gttcaacgcg gcgcaggcac cggcgccggc
     4561 gcagcctgcc gccgccgctc cggcctctcc gctcatggcg cgcgccatgc gcctcctcgg
     4621 cgaaggcgat atcgccggcg cacggctcct gctcatgcat ctagccgagc aaggcgaagc
     4681 cgacgctgcc tacgagctcg cgcgcacgtt cgatgccgaa tatctggccg agcttggcgc
     4741 gcgcggcgtt ggtccggaca agacgcgcgc cgtgggttgg tacgaatggg cctcggagtc
     4801 gggcagcgtg aaggccgccg agcgcttgaa gatcctcgct agtctctcag actagcttga
     4861 ggtgggccgc agccgctaac cgagggacca aatcgggggg atcggcggct gcggcccaac
     4921 ttttctaggt gacgtggcgg cgtctgagtt cggcaagcag agcctcggcg agcgcgtcgg
     4981 gtgtcgtctt gctggtatcg agggtgagct cagcagcctc tggcacctcg tagtctgaat
     5041 cgatgccggt gaagtggctg atcaaccctg cccgcgcctt gcggtagagc cccttgggat
     5101 cgcgcgcctc gcagatttcg agcggcgtgt cgacatagac ctcgacgaac tcgccctctt
     5161 ggagcagctc gcgcgccatc cgccgttcgg agcggaacgg cgagatgaac gacaccatga
     5221 cgatcagacc ggcctccacg aacagcttgg ccgtctccgc cacgcggcgg atgttctcca
     5281 cccggtctgc gtcggtaaag ccgaggtcct tgttgagccc gtggcggatg ttgtcgccat
     5341 cgagcacgta agtgtgccgg ccgagcgcgt gcagccgctt ctccagcatg ttggcgatgg
     5401 tcgacttgcc cgagcctgac agtccggtga accagagcac acaaggctgc tgacccttca
     5461 gccgcgaccg cgcctgcttg tcgatggaga gcgcctgcca ctggatgttg gtggcacgcc
     5521 gcaagccgaa ctcgatcatc cctgcagcga ccgtgccata ggtgaagcgg tcgatcagga
     5581 tgaagctccc ggtctcggaa ttctcggcgt aaggatcgaa cacgagcgcc ttgccggtgc
     5641 ttaagttgca ggacccgact tcgttgagct cgagtgtctt gcctgcgaca tgctccagat
     5701 tgtcgacatt gagcttgtat ttcaggctcg agatggtggc ggttgtggtt tgacagccgc
     5761 atttgagcgc gtagctgcgg ccgggcaaca tcggctcctc atcgaaccag acaatgtgcg
     5821 ccgcgatctg atcggcgatc gcgggcgcct cctcgatccc gcagacgatg tcgccgcggc
     5881 tggcgtcgac ttcttcggca aaggtgagcg tgattgcatc gccgcgatga gcaacctcaa
     5941 ggtcgccgtc ggcggtgacg atgcgcgcca catgcacgcg cttgcccgaa ggctggatca
     6001 tcacctcatc gcccggcctg accttgccgc tcgcaatcgt gccggcgtat ccacggaagt
     6061 cgagatgcgg ccggttcacc cactgcacgg ggaagcggaa cggctcctcc ttctcctccc
     6121 gtgacacatc aaccgtctcg agatgtccga gcagtgtagg gcctcggtac caatgcatat
     6181 gcgggctagg cacgatcaca ttgtcgccct tcagcgacga taccgggatg caggtgacgg
     6241 cttcaaagcc gagcggctcg gcgaagctcc tgaaatcctc ggcgatcgca tcgaagcgcg
     6301 cctgggtgaa atcgacgagg tccatcttgt tcacggcaag cgcgagcttg cggatgccga
     6361 gcagcgagac gatgcaggcg tgccgccgcg tctgggtgag cacgccgctc cgcgcatcga
     6421 ccaagagcac tgcaaagtcg caattggacg ctccggtcgc catgttgcgg gtgtactgga
     6481 catgccccgg cgtatcggcg acgatgaatt tgcggcgcgg cgtcgagaaa tagcgataag
     6541 ccacgtcgat ggtgatcttc tgctc
//
LOCUS       DATNAE010000146         2104 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_442803, whole genome shotgun sequence.
ACCESSION   DATNAE010000146 DATNAE010000000
VERSION     DATNAE010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442803"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1689
                     /gene="thrS"
                     /locus_tag="VN518_03170"
     CDS             <1..1689
                     /gene="thrS"
                     /locus_tag="VN518_03170"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HXE03605.1"
                     /translation="AHVMAEAVQELYPGTQVTFGPATETGFYYDFARDEPFTPEDLEK
                     IEARMHEIVRRDEPIERSVLPREEAIRFFADKGEKYKAEHIESLPREEEISLYRQGGF
                     IDLCTGPHLPSTGKLGHGFKLMKLAGAYWRGDAKNDQLQRIYGTAWPDEKQLEAYLHQ
                     LEEAEKRDHRRLGREMDLFHQQEEAAGSVFWHPKGWTLWRTLEAYMRRRLEAGGYVEI
                     KTPQLLDRSLWEASGHWEKFREAMFTAESEDKVLALKPMNCPCHVQIFRQGIKSYRDL
                     PLRMAEFGSCHRNEPSGALHGIMRVRAFTQDDAHIFCTEEQITAETIAFCNLLRSVYR
                     DFGFDDVAIKLADRPPVRAGSDEVWDKAEAWLKDAVEAAGLAYTLNPGEGAFYGPKLE
                     FVLRDAIGRDWQCGTLQVDFVLPERLDASYIGEDGQRHRPAMLHRAIFGSMERFIAIL
                     IEQYAGRFPLWLAPVQAVVATITSDADAYAEEVRAACAAAGLRVEADLRNEKINYKIR
                     EHSLAKIPAVLVVGKREAETGAVALRRLGGKDQEVLALGGAIARLTQEAAVPSS"
     gene            1796..>2104
                     /gene="infC"
                     /locus_tag="VN518_03175"
     CDS             1796..>2104
                     /gene="infC"
                     /locus_tag="VN518_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014132831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HXE03606.1"
                     /translation="MTPTRDGPRVNQEINVPRVRLVDERGEMIGVVTRSEALQRALEA
                     GLDLVEVAANADPPVVKILDFGKFKYEEQKRRNEARKKQKVIEVKEIKLRPGIDDHDY
                     D"
BASE COUNT          395 a          655 c          701 g          353 t
ORIGIN      
        1 gcccatgtca tggctgaggc ggtgcaggag ctataccccg gcacgcaggt gaccttcggc
       61 ccggcgacgg agacggggtt ttattacgat ttcgcccgcg acgagccctt tacgcccgag
      121 gatctggaga agatcgaagc gcggatgcat gagatcgtcc ggcgcgacga gccgatcgag
      181 cgttcggtct tgccgcgtga agaggcaatt cgcttcttcg ccgacaaggg cgagaagtac
      241 aaggccgagc atatcgaaag cctgccgcgc gaggaggaga tcagcctcta tcgtcagggc
      301 ggctttatcg atctgtgcac cggcccgcat ttgccctcga ccggcaagct cggccacggt
      361 ttcaaattga tgaagctcgc cggcgcctat tggcggggcg atgcgaagaa tgaccagctt
      421 cagcggatct atgggacggc gtggcccgac gagaaacagc tcgaagccta tctccaccag
      481 ctcgaggaag ccgagaagcg cgatcatcgc cggctcgggc gcgagatgga tctgtttcat
      541 cagcaggagg aggcggccgg ctcggtattc tggcacccga agggttggac gttgtggcgc
      601 acgctcgagg cctatatgcg ccggcggctg gaggccggag ggtatgtcga gatcaagacc
      661 ccgcaacttt tggatcgcag cctgtgggaa gcctccggcc actgggagaa gtttcgcgaa
      721 gccatgttca cggctgaatc ggaggacaag gtcctcgcct tgaagccgat gaactgtccc
      781 tgccatgtgc agatcttccg tcagggcatc aagagctatc gcgatcttcc cttgcgcatg
      841 gccgagttcg gctcctgcca tcgcaacgag ccgtcgggcg cgttgcacgg catcatgcgc
      901 gtgcgcgctt tcactcagga cgacgcgcat atcttctgca ccgaggagca gatcaccgcg
      961 gagaccatcg ccttctgcaa cctgctgcgc tcggtctacc gcgatttcgg cttcgacgac
     1021 gttgcgatca agctcgccga ccgcccaccg gtgcgcgccg gctcggatga ggtatgggac
     1081 aaggccgagg cctggctcaa ggacgcggtc gaggcagccg gcctcgccta tacgctcaac
     1141 cccggcgagg gcgcctttta cgggccgaag ctcgaattcg tgctgcgcga cgccatcggc
     1201 cgcgactggc agtgcggcac gctccaggtc gatttcgtcc tgcccgagcg gctcgacgcg
     1261 agttatatcg gcgaggacgg gcaacgtcac cggccggcca tgctgcaccg cgccatcttc
     1321 ggctcgatgg agcgcttcat cgccatcctg atcgagcaat atgccggccg cttcccgctc
     1381 tggctggcgc cggtccaggc ggtggtggcg accatcacct cggacgccga cgcctatgcc
     1441 gaggaggtgc gggcggcttg cgccgccgcg ggcttgcggg tcgaggccga tctccgcaac
     1501 gagaagatca actacaagat ccgcgagcac agcctggcca agatcccggc tgtgctggtg
     1561 gtcggcaagc gcgaggccga gaccggtgcc gtcgcgttgc gccgcctggg cggcaaggat
     1621 caggaagttc ttgcgctggg cggggccatc gctagattga cacaggaagc ggcagtgccc
     1681 tcgtcttgag ggagccgcac gggactttgg gcggtgctgg cgcatagcgg gccggcgccg
     1741 acggtcccat acgcattttg aaaggagggc ttacatcgcc agactaccgc agaacgtgac
     1801 gccgacccgg gatgggccgc gcgtcaacca agagatcaat gtgccgcgcg tacgcctggt
     1861 cgacgagcgc ggcgagatga tcggcgtcgt gacccgcagc gaggccctgc aacgtgccct
     1921 tgaggccggc ctcgatctcg tcgaggtcgc cgccaatgcc gacccgccgg tggtcaagat
     1981 cctcgatttc ggcaagttca aatacgagga gcagaagcgc cgcaacgaag cgcgcaaaaa
     2041 gcagaaggtc atcgaggtca aggaaatcaa gctgcgtccc ggcatcgacg accacgacta
     2101 cgac
//
LOCUS       DATNAE010000147         4192 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_1650729, whole genome shotgun sequence.
ACCESSION   DATNAE010000147 DATNAE010000000
VERSION     DATNAE010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1650729"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="VN518_03180"
     CDS             <1..489
                     /locus_tag="VN518_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018857286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXE03607.1"
                     /translation="ACILPSPKLLLLDEPTAGVDPKARREFWEKIHRLAAEGVTVLVS
                     THYMDEAERCHEIAYISFGELLARGIANEVIAQSGLSTWIVAGADLHRLVLQLQMCDG
                     VDMVAPFGAELHVSGRDSKALDKAIAEAKKADGAHHWKRGEPSLEDVFIMLMDKSKDN
                     YA"
     gene            549..1658
                     /locus_tag="VN518_03185"
     CDS             549..1658
                     /locus_tag="VN518_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011996116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXE03608.1"
                     /translation="MWAMIVKEFVQMRRDRMTFATMLFVPILQLTLFGFAINTDPKNL
                     PTAVLVRDDGPLTRAVLSAMKNTDYFDFKRQVRSADELDQLVRSGTVQFAIEIPASFD
                     RDVRRGDKPSVLVIADATDPVATGTAISAVQGVIDTALRRELRGPDASITDKSQPFEL
                     VLQRRYNPEGITQYNIVPGLLGVVLTMTMMMFTALAVTREIERGTMESLLAMPIKPIE
                     IMIGKIAPFVLVGFVQMTIILVAAHYVFGVPILGSVPLLITLSTLFAASNLAVGYTFS
                     TVAQNQLQAVQMTFFFFLPNFLLSGFAFPFRGMPQWAQWIGEVLPLTHFIRIVRGIML
                     KGNGFADMQMDVAALAIFMFAAMGLALFRFRKTLD"
     gene            1735..2049
                     /locus_tag="VN518_03190"
     CDS             1735..2049
                     /locus_tag="VN518_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HXE03609.1"
                     /translation="MDAVFKALADASRRQLLDRLHAENGQTLGELCTGLDMTRQAVTK
                     HLAILEGANLVVARRRGREKLHYINPVPINEIAERWIGKYERHRLKALGELKRALEGS
                     DE"
     gene            2042..2512
                     /locus_tag="VN518_03195"
     CDS             2042..2512
                     /locus_tag="VN518_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HXE03610.1"
                     /translation="MSRPSFIYVTYINSTPEKVWQALTDPAFTRKYWVNHRNASDWKV
                     GSEWKHEDYDDASQVDIVGHVVEISPPRHLVLTWASPKDANDPDKVSRVTFDIETCGD
                     GVRLTVTHSELEPDSPMLSGISRGWPLVLSSLKTLLETGEPLPSLLVKKAKQRS"
     gene            2601..2810
                     /locus_tag="VN518_03200"
     CDS             2601..2810
                     /locus_tag="VN518_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012255753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03611.1"
                     /translation="MGALAVGKLASGALAVGAFAIGALAVGALAIGRLEIRKARLREV
                     EIDSLTVRHLKVIEEAAGEPNSAES"
     gene            2834..3316
                     /locus_tag="VN518_03205"
     CDS             2834..3316
                     /locus_tag="VN518_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1772 domain-containing protein"
                     /protein_id="HXE03612.1"
                     /translation="MELRTAPFVVIMLAALSLGVGIGHLMQLPARMGWDQYLWVGSTV
                     QGGTYALSGPLGVLLQVATIIALIVLALLLRRHVAAGFAFTVAAAVMFAIGLLIWWVL
                     VYPVNVELAKWVNGPVPDNWAEWRSLWEWGQAANGLAQLFGFAALMASLLVRKAKQRS
                     "
     gene            3525..>4192
                     /locus_tag="VN518_03210"
     CDS             3525..>4192
                     /locus_tag="VN518_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020083945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXE03613.1"
                     /translation="MASTGQAAVADNHLGLLDKERIIAPLNYNRWLVPPAALAIHLCI
                     GMAYGFSVFWKPLQGALLGADGKAVPECSAAATTFGEKAAGTLQALFTTSCNWSQFDL
                     GWMYTLFFVLLGTSAALWGGWLERVGPRRAGVVAAFCWSGGFLISALGVYIHQLWLMW
                     LGSGIIGGCGLGLGYISPVSTLIKWFPDRRGMATGMAIMGFGGGAMIGSPLATMLMNH
                     YATPT"
BASE COUNT          727 a         1348 c         1326 g          791 t
ORIGIN      
        1 gcctgcatcc tgccttcgcc gaaattgctc ttgctcgacg agccgacggc cggggtcgac
       61 cccaaggcgc ggcgcgaatt ctgggaaaaa attcaccggc tcgccgccga gggcgtcacc
      121 gtgctcgtct ccacccatta catggacgag gccgagcgct gtcacgagat cgcctatatc
      181 tccttcggcg agctgctcgc gcgcggcata gccaacgagg tcatcgccca gtccggcctc
      241 tcgacctgga tcgtggccgg cgccgacttg caccggctcg tcttgcagct gcagatgtgc
      301 gacggcgtgg acatggtggc gccgttcggt gccgagctgc atgtgagcgg acgcgacagc
      361 aaagcgctcg acaaggcgat cgccgaggcc aagaaggccg acggtgcgca tcactggaag
      421 cgcggagagc cctcgctcga agacgtgttc atcatgctga tggacaaatc caaggacaat
      481 tacgcgtgag caccgccgaa gcatataacg gcgccggcgg gcgccgggat cccggcgtca
      541 tcagccgcat gtgggcgatg atcgtcaaag aattcgtgca gatgcggcgc gaccgcatga
      601 ccttcgccac catgctgttc gtgccgatct tgcagctcac gctgttcggc ttcgccatca
      661 acaccgaccc caagaacttg ccgacggccg tgctcgtgcg cgacgacggg ccgctcaccc
      721 gcgctgtgct gagcgccatg aagaacaccg actatttcga cttcaaacgc caggtgcgct
      781 cggcggacga gctcgaccag ctcgtcaggt ccggcacggt tcagttcgcc atcgaaattc
      841 cggcgagctt cgaccgcgac gtgcggcgcg gcgacaagcc gtccgtgctc gtcatcgccg
      901 atgccaccga cccggtggcg accggcaccg ccatctccgc cgtgcaaggc gtgatcgaca
      961 cggccttgcg gcgagagctg cgcgggcctg acgcctctat taccgacaaa tctcagcctt
     1021 tcgagctggt cttgcagcgc cgctacaacc ctgagggcat cactcaatac aacatcgtgc
     1081 ctgggctcct cggcgtcgtg ctcaccatga ccatgatgat gttcacggcg ctcgccgtga
     1141 cgcgcgagat cgagcgcggc accatggaga gcctgttggc gatgccgatc aagccgatcg
     1201 agatcatgat cggcaagatc gcgcccttcg tgctggtcgg cttcgtgcag atgaccatca
     1261 tcctggtcgc ggcgcactac gtgttcggcg tgccgatcct cggcagcgtg ccacttctca
     1321 tcacgctgtc gacgttgttc gcggcctcaa acctcgccgt cggctacacc ttctccaccg
     1381 tcgcccagaa ccagctccag gcggtacaaa tgacgttctt cttcttcctg ccgaatttcc
     1441 tgctgtcagg cttcgctttc ccgttccgcg gcatgccgca atgggcgcaa tggatcggcg
     1501 aggtgctgcc gctcacccat ttcatccgca tcgtccgcgg catcatgctg aagggcaatg
     1561 gcttcgccga catgcagatg gacgttgccg cgctcgccat cttcatgttc gccgccatgg
     1621 ggctggcgct gttccgcttc cgcaagacgc tcgactaggc gccgctactc ctgggacagg
     1681 agcgacaagt cttgacttga caggcaacca aatggttgca tattagaagc ttcgatggat
     1741 gccgtcttca aggcgctggc cgatgcgagc cgcaggcaat tgctggatcg gctgcatgcc
     1801 gagaacggcc agacgcttgg cgagctgtgc acagggctcg acatgacgcg gcaggcggtg
     1861 accaagcatt tggcgatcct tgagggcgcc aatctggtgg tggcgaggcg gcgagggcgg
     1921 gagaagctgc actacatcaa tcccgtcccc atcaacgaga tcgccgagag atggatcggc
     1981 aaatatgagc gccatcggct gaaggcgctc ggcgagttga aacgagcgtt ggagggaagc
     2041 gatgagtaga ccgagcttca tctatgtcac ctatatcaac agcacgccgg aaaaagtgtg
     2101 gcaggcgctg accgaccctg ccttcacgcg aaaatattgg gtcaatcacc ggaacgcttc
     2161 agactggaag gtggggtccg agtggaagca cgaggactat gacgatgcga gccaagtcga
     2221 tatcgtcggt cacgtcgtcg agatctcgcc gccgcggcac ctcgtgctga cctgggcctc
     2281 tccgaaggat gccaacgacc ctgacaaggt ctcccgcgtc accttcgaca tcgagacctg
     2341 tggcgatggg gtccgcctga ccgtaactca ctcagagctt gagcctgact ccccgatgct
     2401 ctccggaatt tccaggggct ggccgctggt gctgtccagc ctgaagacgt tgctcgagac
     2461 cggcgagccg ctgccctcgc tgctggtgaa gaaggccaag cagcggagct gagccgcaga
     2521 cggagtgacg cgcttacgcg cccatttcac tctcgaccaa ggatggagta gagaagggca
     2581 ggctgctttt agcatcggcg gtgggggcgc tcgctgttgg caaactcgca tctggggcgc
     2641 tcgccgtcgg cgcgttcgct atcggcgcct tggcggtcgg cgcgctcgcc atcggcaggc
     2701 tggagatccg caaagcgaga ctccgcgagg tcgagatcga cagcctcacg gtgcgccatc
     2761 tcaaagtcat cgaagaggcg gccggagagc cgaacagcgc cgagtcctag gctccggcga
     2821 gaggggggaa gcaatggaac tcaggaccgc tcccttcgtg gtcatcatgc tcgcggcgct
     2881 ctcgctcggc gtcggcatcg gccatctcat gcagctcccg gcgcgcatgg gttgggacca
     2941 gtatctctgg gtggggtcga cggtgcaggg cggcacctac gcgctctccg gcccgctcgg
     3001 cgtgctgctc caggtcgcca ccatcatcgc gctgatcgtg ctcgcgttgc tgctgcgacg
     3061 ccacgtcgcg gccggtttcg ccttcaccgt cgccgccgcg gtgatgttcg cgatcgggct
     3121 cctgatctgg tgggtcctcg tctatccggt caacgtcgag ctcgccaaat gggtgaacgg
     3181 gccggtccct gacaactggg ctgagtggcg gtccttgtgg gaatgggggc aggcggccaa
     3241 tgggcttgcc cagctctttg gcttcgccgc gctcatggcc tcgctgctgg tgaggaaggc
     3301 caagcagcgg agctgagccg cagccggagt cgcggcgccg cgctgtcacg caagtttcgc
     3361 aaattcattc caattgccgg ggctctggca cactgctgat tccatgccga tgccgattgg
     3421 cgggggcgct tgctaggctc ttgcggtcat cttcaagccc gtccagatta cccgccataa
     3481 caaacggggg tgcgccaccg gcatctccga gggagagacg cgcaatggca agcaccggtc
     3541 aggcggcagt tgccgacaat catctcgggc tcctggacaa agagcgcatc attgcgccgt
     3601 taaactacaa ccgttggctc gtgccgccgg cagctctcgc cattcatctt tgcatcggca
     3661 tggcctatgg cttcagcgtg ttctggaagc cgctgcaagg cgcgctcttg ggcgccgacg
     3721 gcaaggcggt tcccgaatgc tcggccgccg cgaccacgtt cggtgagaag gcggcgggca
     3781 cgctgcaggc gctcttcacc acgagttgca actggagcca gttcgatctc ggctggatgt
     3841 acaccctgtt cttcgtgttg ctcggcacct ctgctgccct ctggggcggc tggctcgaac
     3901 gggttgggcc acgcagagcc ggcgtggtcg ccgccttctg ctggagcggt ggctttctga
     3961 tttcggcact cggcgtctat atccaccagc tttggctgat gtggcttggc tcgggcatca
     4021 tcggcggctg tggcctcggc ctcggctata tctcgccggt ctcgaccctg atcaaatggt
     4081 ttcctgaccg ccgcggcatg gcgaccggca tggccatcat gggcttcggc ggcggcgcca
     4141 tgatcggctc gcccctcgcc accatgctga tgaaccacta cgccacgcca ac
//
LOCUS       DATNAE010000148         3437 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_1948245, whole genome shotgun sequence.
ACCESSION   DATNAE010000148 DATNAE010000000
VERSION     DATNAE010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1948245"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..706)
                     /locus_tag="VN518_03215"
     CDS             complement(110..706)
                     /locus_tag="VN518_03215"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HXE03614.1"
                     /translation="MRIAGLVLAGGLSSRMAPRNKLLERVGGEPMVRHVAAVAIASGT
                     QPVIVVTGHEAAAVRGALRGLAVTIVANPDYADGLSTSLRAGLRALPQGIDGALILLG
                     DMPEVESSVLTALVAAFTGESTICVPVRHGRRGNPVLWGSAHFAEMMQLTGDSGAKPL
                     MARHETHLIEVEVATGSIFDDVDAPEDLARLKRSGATS"
     gene            complement(703..1728)
                     /locus_tag="VN518_03220"
     CDS             complement(703..1728)
                     /locus_tag="VN518_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HXE03615.1"
                     /translation="MSAGDPSVIPSALDWLEEGRRVALATVIQTWGSAPRPVGSQLAI
                     DGGGNFLGSVSGGCVEGEVIAEAEEVLLTAEPKTLEFGVEDGTAWKAGLACGGTIRIF
                     VEPLEGGAGGGITVLHRLAGDIAARRKVALVTELANGMRRLAHAPDDLGDDLAPALTE
                     AFRLDRSMALPGRNGEIFINVFAPTTRLVIVGAVHVAQALVPMAGALGYEVLIVDPRA
                     AFATQERFGDVRIEHDWPDEALPASGLDAATAVVALSHDAKLDDAALISALRSDAFYV
                     GALGSRKTHAGRVERLAQAGITSADIARIHAPIGLDIGAQGAAEIALSIIAEITAVAR
                     GKSGARP"
     gene            1917..2726
                     /locus_tag="VN518_03225"
     CDS             1917..2726
                     /locus_tag="VN518_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HXE03616.1"
                     /translation="MKQPRSAEEIVNWSPGYAWLAGGTWLFSTPQIATDTLIDLRSLR
                     WPALAASSTGLDIAATCTIAELYGFQPAAEWTAAPLFQLCARSFLASFKIWNAATVGG
                     NICMSLPAGPMVTLATALEARYTLLPRDGATREVAAIDFVTGNHANVLAPGELLRNIH
                     LPARALRKRFASHRSSLTHLGRSAVFLIGTRSPDEDDLLLTITAATPRPVQIGFDHTP
                     SAAELRHAIDEAVPAYGYFDDVHGSPAYRRRLTYYYAEQIRAELTISGAKA"
     gene            2723..>3437
                     /locus_tag="VN518_03230"
     CDS             2723..>3437
                     /locus_tag="VN518_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HXE03617.1"
                     /translation="MNFAVNGRTFSAEPEPGQCLRTFLRQLGFFGVKKGCDQGDCGAC
                     TVWLDGVPFHSCLVPAFRGAGHEITTIEGLARDGRLHPMQQAFLDAQAFQCGFCAAGM
                     IMTAATFDGEARQDLPHRLKGNLCRCTGYHSIDDALHGVVNVEEEAGGEDACGRSLRN
                     PFAEAIVTGEARYTLDVPADGALHLKVLRSPHAHARITSIKRDKALAVPGVVAVFTWE
                     DVPRKLYSTATHEDHLVDPD"
BASE COUNT          561 a         1196 c         1105 g          575 t
ORIGIN      
        1 cagttggtca ggccctcgcc gcaagcgacc aacatgccgg tcaagaagct cggcacggtc
       61 gtggcgaagg caggttcggt gccgaatgtg cttggcggcg actgagggct tagctcgttg
      121 cgcctgatcg cttaaggcgc gcaaggtcct caggtgcgtc gacgtcgtcg aagatgctgc
      181 cggtcgcgac ttcgacctcg atgaggtgcg tctcgtgccg ggccatcaga ggcttggcgc
      241 cactgtcgcc ggtcaattgc atcatctcgg cgaagtgggc gctgccccat agcaccgggt
      301 tgccccgccg cccgtggcgc acgggcacgc agatcgtgct ttcgccggtg aaggccgcca
      361 caagagcggt aagcaccgag gattcgacct ctggcatgtc gccaagcagg atcagcgccc
      421 cgtcgatgcc ctgaggcaag gctcgcaagc cagcgcgaag cgaggtgctg aggccatcgg
      481 cgtaatcagg gttggcgacg atggtgacgg cgagcccgcg cagcgccccc ctgaccgccg
      541 cggcctcatg cccggttacc acgatgacgg gctgcgtgcc gctcgcgatt gcgacggcgg
      601 caacatggcg gaccattggc tcgcccccaa ccctctccag gagcttgttg cgcggcgcca
      661 tgcgcgagga gaggccgccg gcgagcacga gcccagcgat cctcatggac gcgctccgct
      721 cttgccccgt gccacggcgg tgatctcggc gatgatggaa agcgcgatct cagccgcgcc
      781 ttgcgcgccg atatcgagcc cgatcggggc atgaatgcgg gcgatgtcgg cggaggtgat
      841 cccagcctgc gcgagccgct ccacccggcc ggcatgggtc ttcctgctcc ccagcgctcc
      901 gacatagaag gcgtccgagc gaagggctga gatcagcgcc gcatcgtcga gcttcgcgtc
      961 gtggctcagc gccaccaccg cggtggcggc gtcgaggcct gaagccggca gcgcctcgtc
     1021 tggccaatca tgctcgatcc tgacatcgcc gaagcgctct tgcgtggcga aggcagcgcg
     1081 cgggtcgacg atcaaaacct cgtagccgag cgccccggcc atgggaacga gcgcctgcgc
     1141 cacatgcacg gcgccgacga tgacgaggcg cgtggtcgga gcgaagacgt tgatgaagat
     1201 ttcgccattg cgtcccggca aggccatgct ccggtccagg cggaaggcct cggtaagcgc
     1261 cggcgccaaa tcgtcgccaa gatcgtccgg cgcatgcgca agacgcctca tcccgttggc
     1321 gagctctgtc accagcgcta ccttgcgtcg cgccgcgatg tcgccggcga gtcggtgcag
     1381 caccgtgatc cccccgccgg ctcctccttc cagcggctcg acgaaaatgc ggatggtgcc
     1441 gccacaggcg agcccagcct tccaggcggt gccgtcctcg acgccgaatt cgagcgtctt
     1501 cggctcggcc gtgagaagca cctcctccgc ctcggcgatc acctcgcctt caacgcagcc
     1561 accagagacc gagccgagga aattccctcc cccgtcgatc gctagctggc tgccgaccgg
     1621 ccttggcgcc gagccccagg tctgaatgac ggtggcaagg gccacgcggc ggccctcttc
     1681 cagccagtcg agcgcgctcg gaatgacgct tggatcgccg gcactcatcg cttgctccgc
     1741 aactccggtt ctcgacagtt taggccccga ggccaggtct tatagcattg accgcgagcg
     1801 caaaaatgca gctagaatgg ttttcgagga agcgcttggg gaggatgccg acgcaagctg
     1861 catgggattg cagcgggccg ggtcgctgca acgtgaatct gaacaccatc agagaagtga
     1921 agcagcctcg ctccgccgag gagatcgtta attggagccc tggctatgct tggctcgccg
     1981 gaggcacctg gctgttctcc acgccgcaga tcgcgaccga caccctcatc gaccttcgct
     2041 cgctgcgctg gccggcgctt gccgcttcca gcaccggtct cgacattgcc gcgacctgca
     2101 ccattgccga gctgtatggc ttccaaccgg cggcggagtg gaccgcggcg ccgctgtttc
     2161 agctctgcgc ccgctccttt ctcgcctcgt tcaagatttg gaacgcggcc acggtcggcg
     2221 gcaatatctg catgtcgttg ccggcagggc cgatggtgac gctcgcgacc gcgctcgagg
     2281 cccgttacac gctcctgccc cgcgacgggg ccacgcgtga ggtggccgcg atcgatttcg
     2341 tcacaggcaa ccacgccaat gtgcttgcgc ccggcgagct cttgcgcaac atccatctgc
     2401 ctgcccgcgc gctccgcaag cgcttcgcct cgcaccgctc ctcgctgacc catctcggcc
     2461 gctccgccgt gtttctcatc ggcacgcgga gcccggacga ggacgatctc ctgctgacca
     2521 tcacagccgc cacgccccgg ccggtgcaga tcggcttcga ccacacgccg tccgccgccg
     2581 agctgcgcca tgccatcgat gaggccgttc cggcctacgg ctatttcgac gacgtgcatg
     2641 gctccccggc ctatcggcgg cggctcacct attattatgc cgagcaaatt cgtgccgagc
     2701 tgacaatctc cggagcgaag gcatgaattt cgccgtgaac ggcaggacct tctcggcgga
     2761 gcctgagcct gggcagtgcc tcaggacctt cctgcgccag ctcggcttct tcggcgtcaa
     2821 gaagggctgc gaccagggcg actgcggcgc ctgcaccgtc tggctcgacg gcgtgccctt
     2881 ccattcctgc ctggtgcctg ccttccgcgg cgccgggcac gagatcacca ccatcgaagg
     2941 gctcgcccgc gacggcagac tgcacccgat gcagcaggcg tttctcgacg cgcaggcctt
     3001 ccaatgcggc ttctgcgccg cgggcatgat catgacggca gcgaccttcg acggcgaagc
     3061 gcggcaggat ctgccgcacc ggctgaaggg caatctttgc cgctgcaccg gctatcactc
     3121 gatcgacgat gcgctgcacg gcgtggtcaa tgtcgaggag gaggcaggtg gagaagacgc
     3181 ctgcggcagg agcctgcgca atccgtttgc agaagcgatc gtcaccggcg aagcgcgcta
     3241 cacgctcgac gttccggcgg acggcgcgct tcatctcaaa gtgctgcgct cgccgcatgc
     3301 gcatgcgcgc atcacttcga tcaagcgcga caaggctctc gcggtgcccg gcgtggtcgc
     3361 cgtgttcacc tgggaggacg tgccgcgcaa gctctactcc accgcgacgc acgaggatca
     3421 tctggtcgat cccgacg
//
LOCUS       DATNAE010000149        18201 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2853820, whole genome shotgun sequence.
ACCESSION   DATNAE010000149 DATNAE010000000
VERSION     DATNAE010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 18201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18201
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2853820"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..1514)
                     /locus_tag="VN518_03235"
     CDS             complement(135..1514)
                     /locus_tag="VN518_03235"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HXE03618.1"
                     /translation="MSTRVAGPQGPTIESRDELVAYLEAGNKPMADWRIGTEHEKFGF
                     YRQGHTPVPYDGDRGIRALLEGMKRRFCWDGVMERGNIIALQRPDCPKGGVISLEPGG
                     QLELSGSPLASLHETDEELRHHLSEVTEIDNELGIGFLGIGFSPKWTLAETPVMPKER
                     YRIMKRYMPTRGRLGLDMMFRTATVQVNMDFSSEADMVKKLRVGLALQSVATALFANS
                     PFTDGKPNGYQSYRAEIWRDTDPDRTGLLPFAFEDGMGFERYVDYALDVPMYFVYRDE
                     SYIDVAGASFRHFLDGKLGPLPGVLPTLDDWADHLTTLFPDVRLKRFLEMRGADAGDL
                     RQLCALPALWVGLLYDSEALDQACALIADWTAAERQALRDSVPRRGLATPFRRGALLD
                     VAREVVTIALGGLRRRARVGRNGDERAALEPLLETVEAGRSPSDRLLADYRGAWHGNI
                     DRLFDTQAF"
     gene            complement(1600..2334)
                     /locus_tag="VN518_03240"
     CDS             complement(1600..2334)
                     /locus_tag="VN518_03240"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018065519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HXE03619.1"
                     /translation="MPSKPQRLFVKSRLGPGVTIELEPPQAHYLINVLRCKSGGEILL
                     FNGKDGEWRGGLSGATKRRASVTLGRQTRPQVSAPDLHYLFAPLKRARLDYMAQKATE
                     LGASLLKPVITRRTVAERVNKARLLANAVEAAEQCGILWVPEVAEPEPLASVLDRWDE
                     KRVLVFADEAAPAASPLAALASKTPRPLAVLIGPEGGFEEEERALLLGKPFVLPISLG
                     SRVMRADTAAVAALALVNATIGDWRS"
     gene            2387..3340
                     /gene="ubiA"
                     /locus_tag="VN518_03245"
     CDS             2387..3340
                     /gene="ubiA"
                     /locus_tag="VN518_03245"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HXE03620.1"
                     /translation="MPRPVAQGKASRVADARATNLVDRLAPAALLPYLRLARVDRPTG
                     FWLLAIPCFWSVALASRSIGADYPDAWLLVLFAIGAVVMRAAGCTYNDIIDKDIDAKV
                     ARTQGRPLPSGEVSERSATIFMLVLCMIGLAVLLSFNAATVWLGLCVVPLVALYPLVK
                     RYSYWPQGVLGLAFNWGALLGYSAVLGRLELPAVVLYAGAIAWTIGYDTIYAHQDRDD
                     DELLGLKSTALRFGRATKAWLSGFYALAWLGITVAGLLAGAEIVFLLGMAAAGAHLFW
                     QVATLDIDDPDNCLQRFRSNRDFGLIVFAAIVLDLVLASRF"
     gene            3545..4366
                     /locus_tag="VN518_03250"
     CDS             3545..4366
                     /locus_tag="VN518_03250"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HXE03621.1"
                     /translation="MTALSPASAAPPHTLEEDFRLLKTAVEDAGKLAHSYFRQDLKVK
                     RKHDGTEVSEADFAVDDLLKSTLLGGRPNYGWLSEETEDDPDRLEHRFVWMVDPIDGT
                     NAFLRHVPEWTISAALVQEGKPVLGAVYNPATGEYFHAIRDGGAFLNDAPIHVSTRSD
                     LEGALFIASGGLFKKRIWKEPWPGVESRWVNSVAYRLALVAAGRADATVSLSAKCDWD
                     LAAGVLLVEEAGGVVTDHHGHAFRFNLPNPRFPSLVAAPPLLHAKLIERTSRVDL"
     gene            4415..4699
                     /locus_tag="VN518_03255"
     CDS             4415..4699
                     /locus_tag="VN518_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4170 domain-containing protein"
                     /protein_id="HXE03622.1"
                     /translation="MAAKQGIKAETRQLLHLVFGGELTTLDRIEFSNLKGLDIVGIYP
                     NYAEAYAAWRDAAHRSVDDAHMRYFIVHLHRLLDPETETGAAPKALGFSP"
     gene            4780..6705
                     /locus_tag="VN518_03260"
     CDS             4780..6705
                     /locus_tag="VN518_03260"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXE03623.1"
                     /translation="MKDQLFSDARMVTRGADELQTHYYTTGEIVMRIWREHLRPRLGL
                     LLLASAAMLLTAATTGAIPFIIQKTADDVFVAKNQQMIYWVTAAIVVVTIVKAISEYV
                     ADVTVAYLGHRFIADLRIEMFAKLASADLSFIQTVHSGRLLSSFLNDANLIRQTASRS
                     IVTLGENYLKVVILVATMFYMDARFAFMILLFMPFAWFLLGRQRRKMRKSTTKSLQET
                     GDLSALITQTLRGMRVVRAYRQEPKEEARAAHTINRALEFTMRGTRARALSSPSVELL
                     TGLGFALAIYFAGTKGVRGDLTLGQFMGFMTSALLIYGPLKSAATLQTSLQEGVAAAS
                     RVFSIVDRETQLVEAADAKPLVLKRGQIEFDNISFAYEANNPVLKGVSLTVPSGRTVA
                     LVGPSGSGKSTLVNLCLRFFDPEKGKVAIDGQDIALVTVASLREAIGLVTQDPVLFDD
                     TIRANIAYGTKPEDEAQVIAAAKAAAAHDFIMGLPKGYDTRVGEAGSSLSGGERQRIA
                     IARAIYKDAPILLLDEPTSSLDSEAEAKVQTALEGLMRGRTVLMIAHRLSTVKKADLI
                     CVLDQGRIVEVGRHEELVAKGGLYTRLHGTQFGIAAHAPESENGKAGDGRAEDAGLEG
                     ASVGDADDEDAVAVVGS"
     gene            6721..8742
                     /locus_tag="VN518_03265"
     CDS             6721..8742
                     /locus_tag="VN518_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HXE03624.1"
                     /translation="MAKRLFSSESVVAFGSAVAAGYIRLVYNTSEVRRDPPDTDAKLF
                     AEHPQIFAMWHGQFGMLPKIKPDRPADVAAMVARHGDAELIAGVLSRFGMRLIRGAGA
                     GYRRRNRGGAAALRESLKALQQGTTVAMTADVAPGPARKAGNGIVMLAKLSGRPIVPC
                     AMATNRYLTLSNWSAFTINLPFSKLGIVVGDPVRVPEDADSAALEAARIAVERGLNQA
                     TARAYELAGATDPLLPAVQVKDAPKPAFALRAYRALTRYAAPVAPLLLAWRTRIGKEE
                     PARRPERYGEASVPRPSGFLVWFHAASVGEANAVLPVIDAIAAEHPEIGLLLTTGTVT
                     SAKLARARLPKGAVHQYVPLDNQDYVRRFLKHWQPDLAVFVESEIWPNLVLETNALNV
                     PLVLVNGRMSFRSFRRWRNRPGLSRPLFSAFGLVLAQNDRFAQRFTALGTPRTVAVGN
                     LKADAPPPPVDLAGHKKLAAALAGRTVWLAASTHPGEDDIAAVAHLAMKKARPDLLTV
                     IVPRHPERGPFIARLLEGANLKVALRSEGKLPEAGTDIYIADTIGELGLFYNLVPVAL
                     IGGSLVPHGGQNPVEAIKLGAAVVTGPHWRNFADAYEELLASGGAVQVSDAQSLATTA
                     LLLLENAQARGRIMSRAEGAIARLGGALPRTISELEQFLPPKATPQHAS"
     gene            8732..9751
                     /gene="lpxK"
                     /locus_tag="VN518_03270"
     CDS             8732..9751
                     /gene="lpxK"
                     /locus_tag="VN518_03270"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HXE03625.1"
                     /translation="MPLRAPFWWYRRAGVLASALSPLGAIYGRMAERKAAEVKPYRSR
                     LPVICIGNFTVGGGGKTPTAIAVAEVLKEAGERPCFLTRGYGGKVQGPVLATKGQSAT
                     EIGDETLLLLAHAPTVVSADRAAGAKLIEGTDATVIVMDDGFQNPQLVKDFSLIVVDT
                     QVGLGNGLVMPAGPLRAPLDKQMPRADALLLIGDGGRAAPLATSFEAVNKPVLKARMA
                     PRGDGRWLGVLPVIGFAGIANPKKFYATLSQNGARLTGTRSFPDHHPYTERQAERLLR
                     WAREWNAMLVTTEKDWVRLPEDDGTALSELRFRSRPLLVAIEFGEGNRLKRLLVEAIE
                     KRRTG"
     gene            complement(9748..9975)
                     /locus_tag="VN518_03275"
     CDS             complement(9748..9975)
                     /locus_tag="VN518_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2093 domain-containing protein"
                     /protein_id="HXE03626.1"
                     /translation="MNRFERNIGFKGEAKLKYLDGEVQVLTPGDFVRCAVTGRTIPLD
                     ELRYWSVELQEAYADAAASLKRYRETHPTPA"
     gene            complement(10052..11482)
                     /gene="xseA"
                     /locus_tag="VN518_03280"
     CDS             complement(10052..11482)
                     /gene="xseA"
                     /locus_tag="VN518_03280"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010968483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HXE03627.1"
                     /translation="MADATTNLSTNLVEYTVSELSFALKRTVEEAYGLVKLRGEISNF
                     NGQHSSGHCYFKLKDENAVIDALIWRSTFQRLKFKPEQGLEVVVKGRVTTYPNKSTYQ
                     IVIDALEPAGIGALMALLEQRKRALAAEGLFDAHCKLPLPYLPRIVGVVTSPTGAVIR
                     DILHRLADRFPCHVLVWPVRVQGETCALEVAAAIHGFNGLIVGAAVPRPDLIIVARGG
                     GSLEDLWGFNEEIVARAVAASTIPLISAVGHETDWTIIDLVADYRAPTPTAAAERAVP
                     VRADLHIQVTGFGLRVLSCLRRLVDGQRNLLLALARGLPRRSEILALPRQRFDGVSAR
                     LGRALSANAHAHRVALARAATRLRPHLVRNAARGGRDRLIHLDRSGRRALPALVARSR
                     HRLDARIQLLNSLGYHNVLARGFTLTRDSEGRMLRRAADVHRGERLDIEFADGHVPAQ
                     ADGGDRKATASPKRARTRDEKQGTLL"
     gene            11560..12888
                     /gene="purD"
                     /locus_tag="VN518_03285"
     CDS             11560..12888
                     /gene="purD"
                     /locus_tag="VN518_03285"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HXE03628.1"
                     /translation="MNVLLIGGGGREHALAWKLKQSPLLDTLYCAPGNAGIAEVAELA
                     SLDIGDHDAVASFCQEWKIGLVIIGPEAPLVAGLTDDLEAHGIKVFGPSQAAARLEGS
                     KGFTKDLCARAGIPTASYARFADAASAKTYVARQTLPIVIKADGLAAGKGVAIAETKA
                     DAEAAIDACFSGAFGVAGSELVIEEFLRGEEASFFALVDGTRALPLATAQDHKRAYDG
                     DQGPNTGGMGAYSPAPVMTPALCHRVMLEIIEPTVRAMADAGTKFKGVLYAGLMIDQA
                     TPKLIEYNVRFGDPECQALMLRLDSDLLPALLATAEGRLDGVKLEWSDDAALCVVMAA
                     KGYPGTYAKGSEIKGLAEAAREPGVQIFHAGTRRDVETRDRVRILADGGRVLGVTAKG
                     CDIREAQARAYAAVDKIDWPGGFCRRDIGWRAVERETEPSEPAGSAAPLQ"
     gene            12956..13294
                     /locus_tag="VN518_03290"
     CDS             12956..13294
                     /locus_tag="VN518_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03629.1"
                     /translation="MAHGSRKTAALFAGAAAIALGLGIAPASAAPLAAPGLAAPHALV
                     EPAQVFRRGRPPIYPYYYAPPRRPGGWSFYFGPVPYAKGDYETQALQRVYPQLNYPPS
                     MRYWTPQSGF"
     gene            13311..15065
                     /locus_tag="VN518_03295"
     CDS             13311..15065
                     /locus_tag="VN518_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015441 - ABC-type beta-glucan transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015775 - beta-glucan transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan ABC transporter ATP-binding protein/
                     permease"
                     /protein_id="HXE03630.1"
                     /translation="MIRLYARVLGLLGPQRKLGWVLALANVALAAAGLAEPVLFGRII
                     DALASAQGFGGTPDWPKLTPLVTAWVGFALFLILCGALVALYADRLAHKRRQVVLSDY
                     FEHVLQLPLSFHSATHSGRLMKVMLAGTDTLWWLWLGFFREHLAAIVSIAVLLPLSLY
                     LNWRLGLLLIGLSLIFLALTTFVLRKTETMQKSVERYYSDLAERASDTLGNVALVQSF
                     TRVEDEVRELRAVVDRLLGAQMPVLSWWAVVSTVTKASTTLTMLAILILGLVLFAQGK
                     TTIGGIVMFMSFAGMLIQRLEQAARFVNSVFMNVPRLEEFFGVLDTTTSVKDRKNGVT
                     LKEVRGEVSFDGVTFSYDGKLPAVEDLTFNAEPGETIALVGATGAGKSTALALLYRVY
                     DPRSGKISVDGRDIRGLKLTSLRRNIGVIFQEPLLFNRSIAENLRVGNPDATDEEILA
                     ACRRAEALEFIERHGDGLNANVGERGRMLSGGERQRLSIARALLKDPPILILDEATSA
                     LDAATEAKVTAALKEVMKGRTTFVIAHRLATVRNATRILVFDRGRIVESGSFDDLVAK
                     GGVFAKLAEAQFLAPAAP"
     gene            complement(15231..16697)
                     /locus_tag="VN518_03300"
     CDS             complement(15231..16697)
                     /locus_tag="VN518_03300"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHYT domain-containing protein"
                     /protein_id="HXE03631.1"
                     /translation="MVITGTYNPYLVALSVLVACFASYTALDLGGRVGSARGTARRIW
                     LATAAITLGGGIWSMHFIGMLAFTMPMPMSYDIGLTALSLAVAILATLAGFYVISRHS
                     TSPLRLTLSGTLMGLGIIAMHYTGMAAMRGNVDLSYDPRFVALSVVIAIGAATAALWL
                     AFRHTNPAQRFVAAIVMGLAISGMHYTGMLAAIFHAHSEAGEAASLDQTNLALVIAGI
                     TFLILAAVLLASLSEQTRAEEALRQARADLAHVNRLTTMGELSAALAHEVTQPIAAAV
                     THADASLRWLDRDPPNLDEARAALIMIVKDGTVAKQIIERIRLLFKKGTPQLEWVDVN
                     EVIREMIVLLHGEVTRFSISVRMELARDLPYVVGDRVQLQQVMMNLITNSIDAMKEAD
                     GTRELAIKSGRADDDQLAVCVSDTGVGLPPQLADQIFNTFFTTKPNGTGMGLSICRSI
                     VESHGGRLWATDNIPRGANFHLVLARGEPQGLSRAMVR"
     gene            complement(16905..17336)
                     /locus_tag="VN518_03305"
     CDS             complement(16905..17336)
                     /locus_tag="VN518_03305"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HXE03632.1"
                     /translation="MDLALAEAEAAGLSGEVPIGAVVVSASGEVLARAGNRTLALRDP
                     TAHAELLAIREACATLGSERLTGCDLYVTLEPCAMCAASISFARIRRLYFGAPDPKGG
                     AVEHGPRFFTQPTCHHAPEIYGGIGEARAGELLKRFFAARR"
     gene            17380..>18201
                     /locus_tag="VN518_03310"
     CDS             17380..>18201
                     /locus_tag="VN518_03310"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018238925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HXE03633.1"
                     /translation="MGGKVGAKRRPGQGGKPATVKAAEPMRIAKAMAHAGLCSRRDAE
                     KWIEQGRVAVNGTVLTTPAYVVKPGDDVSVDGKSLPQAEAPRLWRYHKPKGLVTSHKD
                     PQGRKTVFEALPPELPRVVSVGRLDLNTEGLLLLTTDGELARHLELPSTGWLRRYRVR
                     AHGKVGQADLDKLAKGMTIDGVQYGPVEARIDREQGSNVWLTLGLREGKNREVKRLAE
                     ALGITVNRLIRISFGPFSLGDLPVGQVEEVKSRVLMDQLGAKLTGELGLSSENQRL"
BASE COUNT         3116 a         5948 c         5997 g         3140 t
ORIGIN      
        1 gccgccgatc acatccgtct tgggggacgg actagcgtcc catcagtgca gggtagcaga
       61 tgggacggtg acttgattgg ctatatggtt agcgaggacc tgccggtcaa ggcccgcggt
      121 tgcgacgggc gatctcagaa cgcctgggta tcgaacagcc tgtcgatgtt gccgtgccaa
      181 gccccgcgat aatcggccag cagccgatcc gagggagagc gtcccgcctc caccgtctcg
      241 agcagcggct cgagcgccgc gcgttcgtct ccgtttcggc cgacccgcgc ccggcggcgc
      301 aatccgccaa gcgcgatcgt caccacctca cgggcgacat cgagcagcgc gccgcggcgg
      361 aacggggtgg cgagaccgcg ccgtggcacc gaatcgcgta acgcctgacg ctctgccgca
      421 gtccaatcgg cgatgagcgc gcaagcctga tcgagcgctt cgctgtcgta aaggagtccg
      481 acccacagcg ccggcagagc gcagagctgg cggaggtcgc ctgcgtcggc tccgcgcatt
      541 tcgaggaagc gcttcagcct cacatcgggg aacagcgtgg tgagatggtc ggcccagtcg
      601 tcgagagtcg gcagtacgcc cggtagcggg ccgagcttgc cgtcgaggaa atggcggaac
      661 gaggcgccgg caacgtcgat atagctctcg tcgcgataga cgaaatacat cggcacatcg
      721 agcgcgtaat cgacatagcg ctcgaagccc atgccgtctt cgaaggcgaa gggcaatagc
      781 ccggtgcgat cgggatcggt gtcgcgccag atctcggcgc gataggattg gtagccgttc
      841 ggcttgccgt cggtgaaggg cgagttggca aagagagcgg tcgcaaccga ctggagcgca
      901 agcccgacgc gcagcttctt gaccatgtcg gcttcactcg agaaatccat gttcacctgc
      961 accgtcgcgg tgcggaacat catgtcgagg ccgagccgtc cccgcgtcgg catatagcgc
     1021 ttcatgatgc gatagcgctc cttcggcatg accggggtct cggcgagcgt ccatttcggc
     1081 gagaagccga tccccaaaaa tccgatgccg agctcattgt cgatttccgt cacctcggag
     1141 agatggtggc gaagctcctc gtcggtctcg tgcaggctcg cgagcggcga gccggaaagc
     1201 tccagctgcc ctccaggctc aaggctgatg acgccgcctt tgggacaatc agggcgttgc
     1261 agcgcgatga tgttgccacg ctccatgacg ccgtcccagc agaagcgccg cttcatgcct
     1321 tcgagcaagg cgcggatgcc gcgatcgccg tcgtaaggaa ccggcgtgtg accctggcgg
     1381 tagaagccga acttctcgtg ctcggtgccg atgcgccagt cggccatcgg cttgttgcct
     1441 gcttcgagat aagcgacgag ctcatcccgc gactcgatgg tcgggccctg cggcccagct
     1501 acgcgtgtcg acatggggac tagctcgatt gcacgacttt ggtttgcggg gtgaaccgtg
     1561 ccgctcctag cacgtttgcg gctcggcgaa catggcaggt caggagcgcc agtcgccgat
     1621 cgtagcattg acaagggcga gtgcggcgac ggctgcggta tcggcccgca tcacgcggga
     1681 cccgagcgag atcggcagaa caaagggctt gcccaagaga agcgccctct cctcctcttc
     1741 gaacccgcct tccgggccga tcagcacggc aagcgggcgc ggggtcttgc ttgcgagcgc
     1801 cgcaagcggg gaagcggctg gggccgcttc atcggcgaac accagcaccc gcttttcatc
     1861 ccagcggtcg aggaccgagg cgagcggctc aggctcggcc acctcaggca cccacagaat
     1921 gccgcattgc tcggcggcct cgacggcatt ggccaagagc cgcgctttat tgacgcgctc
     1981 ggccaccgtg cggcgggtga tcaccggctt gagcagggag gcgcccagct cggtcgcctt
     2041 ttgcgccata taatcgaggc gggcgcgttt cagcggggcg aacagataat gcaggtcggg
     2101 cgccgaaacc tgcggccggg tctgtcgccc aagcgtgacg cttgcgcgtc gtttggtcgc
     2161 cccgctcaat cccccgcgcc actcgccgtc cttgccgtta aagaggagga tctcgccccc
     2221 gctcttgcat cggagcacgt tgatgagata gtgcgcctgt gggggctcaa gctcgatcgt
     2281 gacgccgggg ccaagtcggg acttgacgaa gagccgctga ggcttggagg gcattctgct
     2341 ggaaacctca tccagttcag ggcaaaagga aaacaggatc gcagacatgc cacgtcctgt
     2401 tgcccaaggc aaagcgagca gggtagccga cgcgcgggcg acgaatctcg tcgaccggct
     2461 ggcgccggcg gcgcttctcc cctatttgcg gctcgcccgc gtcgaccggc cgaccggctt
     2521 ctggctgctc gccatcccct gcttctggtc ggtggcgctc gcctcgcgca gcatcggtgc
     2581 cgactacccc gacgcctggc tgctcgtgct gttcgccatc ggtgccgtgg tgatgcgcgc
     2641 cgccggctgc acctataacg acatcatcga caaggacatc gacgccaagg tcgcccgcac
     2701 gcaaggccgt ccgctgccga gcggcgaggt cagcgagaga tccgccacca ttttcatgct
     2761 ggtgctgtgc atgatcgggc ttgcggtgct cctctccttc aacgccgcga ccgtctggct
     2821 cgggctttgc gtcgttccgc tcgtggcact ctatcctctg gtcaagcgct acagctactg
     2881 gccgcaaggt gtgctggggc tcgccttcaa ttggggcgcc ctgctcggct attccgccgt
     2941 gctcggccgc ctcgaattgc cggcggtcgt gctctacgcc ggagccatcg cctggactat
     3001 cggctacgac accatctacg cccatcagga ccgcgacgac gacgagctgc tcgggctcaa
     3061 atcgaccgcg cttagattcg ggcgggcgac caaggcctgg ctctcagggt tctatgcgct
     3121 tgcttggctt ggaatcaccg tagccgggct cttggccggg gccgagatcg tgttccttct
     3181 tggcatggcg gctgcgggtg cccatctctt ctggcaagtg gccacgctcg atatcgacga
     3241 tccggacaat tgcctgcagc gcttccggtc gaaccgcgat ttcgggttga tcgtattcgc
     3301 ggccatcgtg ctcgatctgg ttctcgcttc gcggttctag gagccggaaa tgaaccgggc
     3361 cccagggatg cacgccctga ggcccgatga cgccttgctg ttagcttcct tgaggctgct
     3421 tgggagagcc tttattgcca cgatcctgcc atagaactta tccgaaccgc ttaagagacg
     3481 tcgttaacgt cgggtatccg gcacttcctg ccgacatggt gtagagagtg ttttcgagac
     3541 caagatgacc gcgctgtcac ccgctagtgc cgctcctcct cacacgcttg aggaggactt
     3601 ccgcctgctc aagaccgcgg tcgaggacgc aggcaagctc gcgcacagct atttccggca
     3661 ggacctcaag gtgaagagga agcacgacgg caccgaggtg agcgaggccg atttcgccgt
     3721 cgacgatctt ctcaagagca cgctcctcgg cggcaggccg aattacggct ggctctcgga
     3781 ggaaaccgag gacgaccccg accggctcga gcaccgcttc gtgtggatgg tcgatccgat
     3841 cgacgggacc aacgcctttc tccgccacgt gccggagtgg accatctcgg cggcgctggt
     3901 gcaggagggc aagccggtgc tcggcgcggt gtacaatccc gccacgggcg agtacttcca
     3961 cgccattcgc gatgggggcg ccttcctcaa cgacgcgccc atccacgtga gcacgaggag
     4021 cgatctcgaa ggcgctctgt tcatcgcgag cggcggcctg ttcaagaagc gcatctggaa
     4081 ggagccttgg cctggcgtcg agtcgcgctg ggtcaattcc gtcgcctatc ggctcgcgct
     4141 cgtggccgca ggccgcgccg atgcgaccgt gtcgctctcg gcgaaatgcg attgggactt
     4201 ggcggccggc gtgctgctgg tcgaggaggc aggcggggtc gtcaccgatc atcatggcca
     4261 cgctttccgc ttcaatctgc ccaacccccg cttcccgagc ctcgtcgccg ccccgccatt
     4321 gctccacgcc aagctcatcg agcgcacgag ccgcgtagat ttgtgacgcg cttttcggca
     4381 aagtcggcgt tggaccaaaa acacgggata catcatggcg gcaaagcagg ggataaaggc
     4441 ggagacgcgg cagcttctcc atctcgtctt cgggggcgag ctcaccacgc tcgacagaat
     4501 cgagttctcc aacctgaagg ggctcgacat cgtcggcatc tatccgaatt acgcggaagc
     4561 ctacgccgcc tggcgcgacg cagcccatag gagcgtggac gacgcgcata tgcgctattt
     4621 catcgtgcat ctgcaccggc tgctcgaccc cgagacggaa accggcgctg cgcccaaggc
     4681 cttaggtttt tcgccttaaa gggaactcgc cgggcatgct agaaaggctc cgccgggtgc
     4741 gtggccgcaa agcccaaacc agcggaatcg gggaagcgct tgaaagatca actgttcagc
     4801 gacgcgagaa tggtcacgcg gggcgctgac gagctccaga cccactatta caccacgggc
     4861 gagatcgtca tgcgcatctg gcgggagcat ttgcgcccgc gcctcggcct gttgctgctc
     4921 gcgtccgccg ccatgctgct caccgcggcg accaccggcg ccatcccctt catcatccaa
     4981 aagaccgccg acgacgtgtt cgtggccaag aaccagcaga tgatctactg ggtcaccgca
     5041 gcaatcgtgg tcgtgaccat cgtcaaggcc atctccgaat acgtggccga cgtcaccgtc
     5101 gcctatctcg gccaccgctt catcgccgat cttcgcatcg agatgttcgc caagcttgcc
     5161 agcgccgatc tttccttcat ccagaccgtc cattccggac ggctgctctc cagcttcctc
     5221 aacgacgcca atctcatcag gcagacggca agccgctcca tcgtcacgct cggcgagaac
     5281 tatctcaagg tggtcatcct cgtcgccacc atgttttaca tggacgcgcg ttttgccttc
     5341 atgatcctgc tgttcatgcc gttcgcctgg tttctgctcg ggcgccagcg caggaagatg
     5401 cggaaatcga ccaccaagtc gctgcaggag accggcgatc tctcggcgct catcacccag
     5461 actttgcgcg gcatgcgcgt cgtgcgcgcc tatcgccagg aacccaagga agaagcccgc
     5521 gccgcgcaca ccatcaaccg cgcgctcgaa ttcaccatgc gcggcacgcg ggctcgcgcg
     5581 ctgtctagcc cgtccgtcga gctcttgacc ggacttggct ttgccctcgc tatctatttc
     5641 gccggcacca agggcgtgcg gggagacctc acgctcggcc agttcatggg cttcatgacc
     5701 tcggcgctcc tgatctacgg cccgctgaaa agcgcggcga ccttgcagac cagtttgcag
     5761 gagggggtgg cggcggcgag ccgcgtgttc agtatcgtcg accgcgagac gcagctcgtt
     5821 gaagccgccg acgccaagcc gctcgtcttg aagcgcggcc agatcgaatt cgacaacatc
     5881 tccttcgcct atgaggcgaa caacccggtg ctgaaaggcg tgagcctcac cgttccctcc
     5941 ggccgtaccg tggcgctggt cggaccttca ggctcgggca agagcacgct ggtcaacctc
     6001 tgtttgcgtt tcttcgatcc cgagaaaggc aaggtggcga tcgacgggca ggacatcgcg
     6061 ctcgtcaccg tcgcgagctt gcgcgaggcc atcggcctcg tcacccaaga cccggtgctg
     6121 ttcgacgaca ccatccgcgc caatatcgcc tacggcacca agccggagga cgaagcgcaa
     6181 gtcatcgccg cggccaaggc ggcggcggcg cacgatttca tcatggggct gcctaagggc
     6241 tacgacacgc gcgtcggcga agcaggctcc tcgctttccg gcggcgagcg ccagcgcatc
     6301 gccatcgctc gggcgatcta caaggatgcg ccgatcttgc tgctcgatga gccgacgagc
     6361 tcgctcgaca gcgaggccga agccaaggtg cagaccgcgc tcgaaggatt gatgcgcggg
     6421 cgcaccgtgc tgatgatcgc ccaccgcctg tctacggtga agaaggccga tctcatctgc
     6481 gtgctcgatc aggggcgcat cgtcgaggtc gggcgtcacg aagagctggt tgccaagggc
     6541 ggcctctata cgcgtctgca cggaacccag ttcggcatcg ccgcccatgc gccggaaagc
     6601 gaaaacggca aggccggtga cggtcgcgcc gaagacgccg gcctcgaagg cgcaagcgtt
     6661 ggcgatgccg acgacgaaga cgccgtcgcc gtggtcggaa gctaggcaag cgcgtctttc
     6721 atggcaaagc ggctgttctc ctcagagtcc gtggtcgcct tcggcagcgc ggtcgcggcg
     6781 ggctatatcc gcctcgtcta caatacgagc gaagtccggc gcgatcctcc ggacaccgac
     6841 gccaagctct tcgccgagca tccgcaaatc ttcgccatgt ggcatgggca gttcggcatg
     6901 ctgcccaaga tcaagccgga ccggccggca gacgtcgccg ccatggtggc gcgtcacggc
     6961 gacgccgagc tgatcgcggg ggtcctgagc cgcttcggca tgcgattgat caggggcgcc
     7021 ggcgccggct accggcgccg gaatcgtggc ggggcagccg cgcttcggga atcgctcaag
     7081 gcgcttcagc aaggcacgac cgtggccatg accgccgacg tcgctccggg tccggcgcgc
     7141 aaggcgggaa acggcatcgt catgctggcg aagctctcgg ggcgcccgat cgtgccctgc
     7201 gccatggcga cgaatcgcta cctcacgctc agcaattgga gcgcgtttac catcaacctg
     7261 cccttctcga agcttggcat cgtggtcggc gatccggtaa gggtgcctga ggacgccgat
     7321 tcggccgcgc tcgaggccgc ccgcatcgcc gtcgaacgcg gcctcaatca agcgaccgcg
     7381 cgcgcctacg aacttgccgg ggccaccgat ccgttgttac cagccgtaca agtcaaggat
     7441 gccccaaagc ccgccttcgc gcttcgcgcc tatcgcgcgc tgacgcgcta tgccgcgccg
     7501 gtcgcgccgc tgctgcttgc ctggcgcacc cggatcggca aggaggagcc ggcgcgccgg
     7561 cctgagcgtt acggcgaggc gagcgtgccg cgcccatccg gcttccttgt gtggttccat
     7621 gccgcgagcg tcggggaggc caatgccgtg ctgccggtga tcgatgcgat cgccgccgag
     7681 cacccggaga tcggcctctt gctcaccacc ggcacggtga cctcggcgaa gctcgcgcgg
     7741 gcgcggctgc cgaagggcgc cgtgcaccaa tatgtgccgc tcgacaatca ggactatgtg
     7801 cggcggttcc tcaagcattg gcagcctgac ctggcggtgt tcgtcgaatc ggaaatctgg
     7861 ccgaacctcg tgctggagac gaacgccctc aacgtcccgc tcgtgctggt caacggccgc
     7921 atgtcgttcc gctctttccg ccgctggcgc aaccgtcccg gcttgagccg tccgctgttc
     7981 tcggcattcg gcctggtgct tgcccagaac gatcgcttcg cccagcgctt caccgcgctc
     8041 ggcacgccgc gcactgtcgc ggtcggcaat ctcaaggccg acgcgccgcc gccaccggtg
     8101 gatcttgctg ggcataagaa gcttgccgcg gcgcttgccg gccgcacggt gtggctcgcc
     8161 gcgagcaccc atccaggcga ggacgatatc gccgcggtcg cgcatctcgc catgaagaag
     8221 gcgcgaccag acctcctcac cgtcatcgtg ccccgtcatc cggagcgagg gccgttcatc
     8281 gcgcggctgc tcgagggggc gaacctcaaa gtagcgctcc gctcagaagg gaagctgcca
     8341 gaggccggca ccgacatcta catcgccgac accatcggcg agcttgggct cttctataac
     8401 ctcgtgccgg tggcgctgat cggcggctcg ctcgtgccgc atggcgggca gaacccggtt
     8461 gaggcgatca agcttggcgc cgccgtcgtg accggcccgc attggcggaa cttcgccgat
     8521 gcctatgagg agctgttggc ctcgggcggc gcggtgcagg tgagcgatgc gcaaagcctc
     8581 gccactactg ctctgctcct gcttgagaat gcgcaggcgc gcgggcggat catgtcccgc
     8641 gccgagggcg ccatcgctcg cctcggcggt gccttgccgc gcaccatcag cgagcttgag
     8701 caattccttc cgccgaaggc gaccccgcaa catgcctctt agggcgccat tctggtggta
     8761 tcgaagggct ggagtgctgg cctcggcgct ttcgccctta ggcgcgatct atgggcgcat
     8821 ggccgagcgc aaggccgccg aggtcaagcc ctaccgctcg cgtcttccgg tgatctgcat
     8881 cggcaatttc accgttggcg gtggcggcaa gacgccgacg gccattgccg ttgccgaagt
     8941 gctgaaggaa gcgggcgagc ggccctgctt cctcacccgc ggctatggcg gcaaggtgca
     9001 aggcccggtg ctcgcaacca agggccagag cgctaccgag atcggcgacg agacgctgct
     9061 gttgctcgcg cacgcgccga ccgtggtctc cgccgaccgc gcggccggcg ccaagctgat
     9121 cgagggcacc gacgccaccg tcatcgtcat ggatgacggc tttcagaacc cgcagctcgt
     9181 gaaggatttc agcctgatcg tggtcgatac tcaggtcggg ctcggcaatg ggctggtgat
     9241 gccggccggg cctttacggg cgccgctcga caagcagatg ccgcgcgccg acgcgctgct
     9301 gctgatcggc gatggcggca gggctgcccc gctcgccacg agcttcgagg ctgtcaacaa
     9361 gccggtgctc aaggcacgca tggcgccgcg cggcgatggg cgttggctcg gcgtgctgcc
     9421 ggtcatcggt tttgccggca tcgccaaccc gaagaaattc tacgccacgc tgtcgcagaa
     9481 cggggcgcgg ctcaccggca cgcgctcatt ccccgaccat cacccttaca ccgagcgcca
     9541 ggcagagcgg ctgctcagat gggcgcggga atggaacgcc atgttggtca ccactgagaa
     9601 ggattgggtg cgcctgcctg aggacgacgg cacggcgttg agcgagctca ggttccgttc
     9661 gcgccctctt ctcgtcgcca tcgagttcgg cgagggcaac aggctgaagc ggcttctggt
     9721 cgaagccatc gagaagcgcc ggacgggcta agcaggcgtc gggtgggtct cgcggtagcg
     9781 cttcagcgag gcagcggcgt cggcataggc ctcctgcagc tcgacgctcc aatagcgcag
     9841 ctcatcgagc gggatggtgc ggccggtcac ggcgcagcgc acgaagtcgc cgggagtcag
     9901 cacctgaacc tcgccgtcga gatatttcag tttggcttcg cccttgaagc caatgttgcg
     9961 ttcaaaacgg ttcatcaagg ccgtgtcact cgcctcatgt cgaatttcgt tcgatcacca
    10021 tagcgtcgcc gcgcggcggt ggccagcccg cttacagcaa tgtgccctgc ttttcgtcgc
    10081 gcgtgcgtgc acgcttcgga cttgctgttg ccttgcggtc gccaccatcg gcctgcgcag
    10141 ggacatggcc atcggcgaac tcgatgtcca gacgctcgcc gcgatgcacg tcggcggcgc
    10201 ggcggagcat gcggccttcg gagtcgcggg tgagcgtgaa gccgcgggcg agcacgttgt
    10261 gatagccgag cgagttgagg agctggatgc gcgcatcgag gcggtggcgc gaccgcgcca
    10321 ccagagctgg aagagcgcgg cggcctgagc ggtcgagatg gatgagccgg tcgcgccctc
    10381 cccgcgcggc attgcgcacg agatgcggcc tgagccgcgt ggctgcgcgc gcgagcgcga
    10441 cgcgatgggc atgggcattg gcgctgagcg cgcggccgag cctcgcactc acgccgtcga
    10501 agcgctgacg cggcagtgca agaatttcgc ttcgccgcgg caggccgcgg gcgagcgcaa
    10561 gcaatagatt gcgctgccca tcgaccagcc gcctgaggca gctcagcacg cgcaggccga
    10621 agcccgtcac ctggatgtgc agatcggcgc gcaccggcac ggcgcgctcg gcggcggcgg
    10681 tcggcgtcgg cgcgcggtaa tcggcgacga ggtcgatgat ggtccagtcg gtttcatgcc
    10741 ccaccgcgga gatgagcggg atggtgctcg ccgccacggc gcgggcgacg atctcctcgt
    10801 tgaagcccca cagatcttcg aggctgccgc cgccgcgcgc cacgatgatc agatcgggcc
    10861 gcggcacggc ggccccgacg atcagcccgt tgaagccgtg gatggcggcg gcgacctcga
    10921 gcgcgcaggt ctcgccctgc accctgaccg gccagaccag aacgtggcag gggaagcggt
    10981 cggcgagcct gtgcaggatg tcgcggatca cggcacccgt gggcgaagtg accacgccga
    11041 cgatgcgcgg cagatacggg agcggcagct tgcaatgggc atcgaacagg ccctcggcgg
    11101 cgagcgcgcg tttcctctgc tcgagcagag ccatgagcgc gccgatgccg gcaggctcga
    11161 gagcgtcgat gacgatctga taggtcgatt tgttgggata ggtggtcacc cttcccttga
    11221 ccacgacctc gaggccctgc tcaggcttga atttgagccg ctgaaaggtg ctccgccaga
    11281 tgagcgcgtc gatgacggcg ttctcgtcct tgagcttgaa gtagcagtgg cccgagctgt
    11341 gctgaccgtt gaagttggag atctcgccgc ggagcttcac taggccatag gcctcctcca
    11401 cggtgcgctt cagcgcgaag gaaagctcgg agacggtata ttcgacgaga ttggtcgaca
    11461 gatttgtggt ggcgtcggcc attctagatc agcggttcca ttttgcttct gccgcccccg
    11521 tgctagggag gcagcctgac agaccggagc tttaccgcat tgaacgtgct gttgataggt
    11581 ggaggcggcc gcgagcacgc gcttgcctgg aagctcaagc aaagcccgct ccttgatact
    11641 ctctattgcg cccctggcaa tgccgggatc gccgaggtcg cggagctcgc atcgctcgat
    11701 attggcgatc acgacgcggt cgccagcttc tgccaggagt ggaagattgg cctcgtgatc
    11761 atcggccccg aagcgccgct cgtcgctggc ctcaccgacg atctcgaagc ccacggcatc
    11821 aaggtgttcg gcccgtcgca ggccgcagcg agactcgaag gctcgaaggg cttcaccaag
    11881 gatctctgcg cgcgggccgg cattccaacc gcttcctatg cccgcttcgc cgatgcggcg
    11941 tccgcgaaga cttatgtggc gcggcagact cttcccatcg tgatcaaggc tgacggcctc
    12001 gccgcaggca agggcgtggc catcgcagag acgaaggccg atgcggaggc cgccattgat
    12061 gcctgcttct caggcgcctt cggcgttgcg ggatcggaac tggtgatcga ggagttcctg
    12121 cgcggcgagg aagcgagctt cttcgctctg gtcgacggca cgagagcgct gccgcttgcc
    12181 acggcgcagg accacaagcg cgcttacgac ggcgaccagg gaccgaacac cggaggcatg
    12241 ggcgcatact ctccggcgcc ggtcatgacg cccgcgctct gccaccgcgt tatgctcgag
    12301 attattgagc cgacggtgcg cgcgatggct gacgcaggca cgaagttcaa gggcgtgctc
    12361 tatgccgggc tgatgatcga ccaggccacg ccaaagctta tcgaatacaa tgtccgcttc
    12421 ggcgatcctg aatgtcaggc gctgatgctg cggctcgact ccgacctcct gccggcgctg
    12481 ctcgccaccg cggagggccg gctcgacggc gtcaagctcg aatggagcga cgatgctgcg
    12541 ctgtgcgtgg tcatggcggc gaagggctat cccggcactt acgccaaagg ctccgagatc
    12601 aagggcctcg ccgaagccgc gcgcgaacca ggcgtgcaaa tcttccatgc cgggaccagg
    12661 cgcgatgtgg agacacgcga ccgcgtgcgc atcctcgccg atggcgggcg cgtgctcggc
    12721 gtcaccgcga agggctgcga cattcgcgag gcgcaagcgc gcgcctatgc cgccgtcgac
    12781 aagatcgact ggcccggggg cttctgccgc cgcgacatcg gctggcgcgc ggtcgagcgc
    12841 gagaccgagc cgagcgaacc cgctggctcc gccgcgcccc tgcagtaact cattgcggcg
    12901 gcaggcgttg gtgcttacgt atcattccgg acctgaaagc agatcggaga ttctgatggc
    12961 gcacggctca agaaaaaccg cagccctctt tgccggcgct gccgcaatcg cgcttggcct
    13021 cggcatcgcg cccgctagcg ccgcgccgct cgccgcgcca ggactcgccg cgccacacgc
    13081 tctcgtcgag cctgcgcaag tcttcaggcg cggccggccg ccgatctacc cctattacta
    13141 cgcgccgccg cgccgccccg gcggctggag cttctatttc gggccggtgc cttacgccaa
    13201 gggcgactac gagacgcaag cgctgcagcg cgtctatccg cagttgaact acccgcccag
    13261 catgcgctac tggaccccgc aatcagggtt ctgagcctcc ctttcacccc atgattcgcc
    13321 tctacgcccg cgtgctcggc ctgctcggcc cgcagaggaa gctcggctgg gttctggcgc
    13381 tcgccaatgt ggcgctcgcc gccgcgggcc tcgccgagcc agtgctgttc ggtcgcatca
    13441 tcgatgcctt ggcgagcgcg caaggcttcg gcggcacgcc ggattggccg aagctcaccc
    13501 cgctcgtcac cgcttgggtc ggctttgcgc tgttcctcat cttgtgcggg gcgctcgttg
    13561 cgctctacgc cgacaggctc gcccacaagc gccgccaggt cgtgctgtcg gactatttcg
    13621 agcacgtgtt gcagcttcca ctcagcttcc acagcgccac ccattcgggg cggctgatga
    13681 aggtgatgct cgccggcacc gacacgctgt ggtggctgtg gctcggcttc ttccgcgagc
    13741 atctcgccgc catcgtgtcc atcgccgtgc tcctgccgct gtcgctctat ctcaattggc
    13801 ggctcggcct cctgctcatc gggctttcgc tcatcttcct tgcgctcact accttcgtgc
    13861 tgcgcaagac cgagaccatg cagaaatcgg tggagcgcta ctactccgac ctcgccgagc
    13921 gcgcctcgga cacgctcggc aacgtcgcgc tggtgcagag cttcacccgc gtcgaggatg
    13981 aggtgcgcga gttgcgcgca gtggtcgacc gcctgctcgg ggcgcagatg ccggtgctgt
    14041 cgtggtgggc ggtggtctcg accgtcacca aggcgtcgac cacgctcacc atgctcgcca
    14101 tcctgatcct cgggctcgtc ctgttcgcgc aaggcaagac cacgatcggc ggcatcgtca
    14161 tgttcatgag tttcgccggc atgctgatcc agcgcctcga gcaggctgcg cgcttcgtca
    14221 attccgtctt tatgaacgtg ccgcggctcg aggaattctt cggcgtgctc gacaccacga
    14281 cgtcggtgaa ggaccgcaag aacggggtta cgctcaaaga ggtgcgcggc gaggtatcct
    14341 tcgacggcgt caccttctcc tatgacggca agctccctgc cgtagaggac ctgaccttca
    14401 atgcagagcc tggcgagacc attgcgctgg tcggcgccac cggcgccggc aaatcgacgg
    14461 cgctggcgct gctctatcgc gtctacgacc cgagaagcgg caagatcagc gtcgacggcc
    14521 gcgacatccg cggcttgaag ctcacgagcc tgcggcgcaa tatcggggtg atcttccagg
    14581 agccgctttt gttcaaccgc tcgatcgccg agaatttgcg ggtcggcaat ccagacgcca
    14641 ccgacgagga gatccttgcc gcctgccgcc gcgccgaggc gctcgaattc atcgagcggc
    14701 acggcgacgg gctgaacgca aatgtcggcg agcgcgggcg catgctatca ggcggcgagc
    14761 ggcagaggct gtcgatcgcc agggcgctcc tgaaagaccc tccgatcctg atcctcgacg
    14821 aggcgacgag cgcgctcgat gccgcgaccg aggcgaaggt gaccgccgcc ctcaaggagg
    14881 tgatgaaggg ccgcaccacc ttcgtcatcg cccaccgtct cgccacggtg aggaacgcca
    14941 cgcgcatcct ggtgttcgac cgcggacgca tcgtcgagag tggcagcttc gacgacttgg
    15001 tggccaaggg cggcgtgttt gccaaactgg ccgaagcgca gttcctcgcg cccgcggcgc
    15061 cgtagggtgg gcaacggcat tcgcgctcaa gcagcgaagc ggtagcgcga tcgaatttgc
    15121 cgttgcccac caaaatgatg gtgggcacgc tgcgctttgc ccaccctact ctcgtcatgg
    15181 ccgggcttga tccgcctacg cgggccgaag ccgcttccgc tgatccgaac tcaccgaacc
    15241 atcgcgcggc tcagcccctg cggctctcct ctggccagca cgaggtgaaa attggcgccg
    15301 cgcggaatgt tgtcggtcgc ccataaccgg ccgccatgtg actcgacgat ggagcggcaa
    15361 atcgacaacc ccatccccgt cccgttcggt ttggtggtga agaacgtatt gaagatctga
    15421 tcagcaagct gcggcggcag ccccacgccg gtgtcgctca cgcacaccgc cagttgatcg
    15481 tcgtcggcgc gccccgactt gatggcgagc tcgcgggttc cgtcagcctc cttcatggcg
    15541 tcgatgctat tggtgatgag gttcatcatc acctgctgca gctgcacgcg gtcgccgacg
    15601 acgtaaggaa gatccctggc gagctccatg cggaccgata tggagaatcg cgtcacctcg
    15661 ccgtgcagca agacgatcat ctcgcgaatg acctcgttga catcgaccca ttcaagctgc
    15721 ggcgtgccct tcttgaacag caggcgaatg cgctcgatga tctgtttcgc aaccgtgccg
    15781 tctttcacga tcatgatgag tgccgcgcgc gcctcgtcga gattgggggg atcgcgatcg
    15841 agccagcgca gagaggcatc ggcgtgggtg acggcggcgg cgatcggttg ggtcacttcg
    15901 tgagccaaag ctgccgacag ctctcccatg gtggtcaacc ggttgacatg ggcgagatcg
    15961 gcccgcgctt ggcgcagcgc ttcttcggcg cgcgtctgct cggacaggga ggcgagcaac
    16021 acggcagcca ggatcaggaa ggtgatgccg gcgatcacca gcgcgagatt ggtctgatcg
    16081 aggctcgcgg cttcgccggc ctcgctatgg gcgtgaaaga tggccgccag catcccggtg
    16141 tagtgcatgc cggaaatggc gaggcccatg acaatggcgg caacgaatcg ctgagcggga
    16201 ttggtgtggc ggaacgcgag ccacagcgcc gcggttgccg cgccgatggc gatgaccacg
    16261 gagagcgcaa cgaagcgagg gtcgtagctg agatcgacat tcccgcgcat ggccgccatg
    16321 ccggtgtaat gcatggcgat aatgcccaag cccatgaggg tgccgctcaa ggtgaggcga
    16381 agcggcgagg tgctgtggcg gctgatgacg taaaagccgg ccaaggttgc gaggatggcg
    16441 accgcgagcg aaagggcggt caagccgatg tcgtaggaca tcggcatcgg catggtgaag
    16501 gccagcatgc cgatgaaatg catcgaccat attccgccgc ccaaggtgat cgccgcggtg
    16561 gcgagccaga tgcggcgcgc agtgccgcgc gcactgccga cccggccgcc aagatcgagt
    16621 gccgtgtaag acgcaaagca ggcgaccaga acggacagag caacgagata cggattgtat
    16681 gtcccggtaa taaccaataa acgcccccaa ctcggatcag agagaaatgg gcgacgtgtc
    16741 tgctagctcc gaaagtgtac ttcaatacta tcccgcgggc tatcggctcg acttgaatat
    16801 tggctgctta cgggccggga cctcgggact tggcggcaat cgactttaga acgttcttgg
    16861 cgggagacgc gttccactat gtcgcggggg cgccgtgtca ttttctagcg gcgggcggcg
    16921 aagaagcgtt tcagaagctc gccggcgcgg gcctcgccga tgccgccata gatttccggc
    16981 gcgtggtggc aggtcggttg ggtgaagaag cgcgggccat gctcgacggc gccgccttta
    17041 gggtctggcg cgccgaaata gagcctgcgg atgcgggcga aggagatgga agcggcgcac
    17101 atggcgcatg gctcgagcgt cacatagagg tcgcagccgg tgagccgctc gctgccgagc
    17161 gtggcgcagg cctctctgat ggcgagcagc tcggcgtgtg cggtcgggtc gcggagcgca
    17221 agcgtgcggt tgccggcgcg tgccagcacc tcgcccgagg cgctcaccac cacggcgccg
    17281 atgggcacct cgcccgaaag gccggcagcc tcggcctcgg caagggcgag atccatgggc
    17341 cgcttgcttg gggtcgccac atctgcaagc ttttcatcca tgggcgggaa agtgggcgcg
    17401 aagcgaaggc ctggtcaagg gggcaagccc gcgaccgtaa aggcggcgga gcccatgcgc
    17461 atcgccaagg ccatggccca tgccggactt tgctcgcggc gcgacgccga aaagtggatc
    17521 gagcaaggcc gcgtcgcggt caacggcacg gtgctgacca cgccagccta tgtggtcaag
    17581 cctggggacg acgtctccgt tgacggcaaa tcgctgcctc aggccgaggc gccgcggctg
    17641 tggcgctatc acaagcctaa agggctcgtg accagccaca aggacccgca agggcggaag
    17701 accgtgttcg aggcattgcc gcccgagctt ccccgcgtgg tctcggtggg gcggctcgat
    17761 ctcaataccg aagggcttct gctgctcacc accgacggcg agcttgcgag gcatctggag
    17821 ctgccgagca ccggctggtt gcgccgctat cgcgtgcggg cacacggcaa ggtcggccaa
    17881 gccgacctcg acaagctggc caagggcatg accatcgacg gcgtacagta cgggccggtg
    17941 gaagcgagga tcgatcgcga gcaagggagc aatgtctggc tgacgctcgg gcttcgcgaa
    18001 ggcaagaacc gcgaggtgaa gcggctcgcg gaggcgctcg gcatcaccgt caaccgcctc
    18061 atccgcatct ccttcgggcc gttctcgctc ggcgatctgc cggttggcca agtggaagag
    18121 gtcaagtcgc gggtgttgat ggaccaattg ggcgcgaagc tcaccggcga gcttggattg
    18181 agcagcgaga accaaaggct g
//
LOCUS       DATNAE010000150         4869 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_4358081, whole genome shotgun sequence.
ACCESSION   DATNAE010000150 DATNAE010000000
VERSION     DATNAE010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4869
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4358081"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="VN518_03315"
     CDS             <1..735
                     /locus_tag="VN518_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXE03634.1"
                     /translation="LAQNGVMNEGEVATRLGLPGIPREAETIMLERDLRLAELTCGRY
                     HAGQISCRASLDIVRAAKERGLPITCGVSINHLTLNENDIGPYRTFFKMRPPLRSEDD
                     RAAMVEGIASGDIDVIVSAHDPQDADGKRRPFAEAADGAIGVETLLSAALRLYHDGSV
                     DLSTLLRALTANPARLLGLPSGRLAKGAPADLVLIDLDTPFVVNPELLRTRSKNTPFD
                     EARLQGRALMTLVAGRAVYNYGEHGS"
     gene            764..1369
                     /gene="plsY"
                     /locus_tag="VN518_03320"
     CDS             764..1369
                     /gene="plsY"
                     /locus_tag="VN518_03320"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HXE03635.1"
                     /translation="MLAADWSLYDLLALGLGYLLGSIPFGLIVTKLAGHGDLRDIGSG
                     NIGATNVLRTGNKRLAALTLLLDLLKGTAAVLIGARFGPDAAILGGLGAFLGHLFPVW
                     LGFRGGKGVATYIGVLLGLYWPGAIAFCAIWLLVALVTRYSSLSALAASAAIPLILVL
                     VGNTRIALLFLFFTVLLYWRHADNIGRLLRGEEGRIGAKKT"
     gene            1383..2521
                     /gene="dprA"
                     /locus_tag="VN518_03325"
                     /pseudo
     CDS             1383..2521
                     /gene="dprA"
                     /locus_tag="VN518_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003533029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
     gene            complement(2525..2671)
                     /locus_tag="VN518_03330"
     CDS             complement(2525..2671)
                     /locus_tag="VN518_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008936804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03636.1"
                     /translation="MTRREAADYIATLVDELKSIAQAAGLPLIAYLLAMAKEEAQVER
                     ARDD"
     gene            2974..>4869
                     /gene="topA"
                     /locus_tag="VN518_03335"
     CDS             2974..>4869
                     /gene="topA"
                     /locus_tag="VN518_03335"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HXE03637.1"
                     /translation="MNIVIVESPAKAKTVNKYLGSGYRVLASYGHVRDLPSKNGSVLP
                     DKDFEMHWDVEPKAAKRLDEIAKAVKGATKLILATDPDREGEAISWHVYQILNRKKAL
                     DKVAVERVVFNAVTKEAVLDAMRHPREIDGPLVNAYLARRALDYLVGFTLSPVLWRKL
                     PGARSAGRVQSVALRLVCDREAEIEAFKAQEYWSLAAELATLKNETLLARLTAIDGKK
                     LEKFDIADEATAMRLKAALEGGSFIVQSVESKAQRRHPAPPFTTSTLQQEASRKLGFS
                     PRHTMQIAQRLYEGVDMGGESEGLITYMRTDGVQIAPEAIAAARRTVEQLYGRNYVPA
                     SPRQYETKAKNAQEAHEAIRPTDFGKPPEKVARYLDDESLKLYKLVWQRTLASQASSA
                     EIERTTAEIGVTGNDGVAYGLRATGSVIRFDGFLKLYEEGTDDVIGEDGALPILAQGD
                     QLKGRKIDAKQHFTEPPPRYSEASLIKKMEELGIGRPSTYASTLNILRDREYVRLDKK
                     RLHPEDKGRLVTAFLENFFRRYVEYDFTADLEEKLDLISAGQLAWKDVLRDFWRDFIA
                     AVHDIGDLRIAEVLDALNDMLGPHIFPGTEAGGDPRACPSCETGRLSLKIGKFGAFIG
                     CSNYPDCR"
BASE COUNT          822 a         1693 c         1477 g          877 t
ORIGIN      
        1 ctcgcccaga acggcgtgat gaacgagggc gaggtggcga cgcggcttgg cctcccgggc
       61 attccgcgcg aggccgagac catcatgctc gagcgcgacc tgcgtctcgc cgagctcact
      121 tgcggccgct atcacgccgg ccagatctcc tgccgtgcgt cgctcgacat cgtccgcgcc
      181 gccaaggagc gcggactgcc gattacctgc ggagtctcga tcaatcatct taccttgaac
      241 gagaacgaca tcggtcccta ccggaccttc tttaagatgc gccccccgct ccgctccgag
      301 gacgaccgtg ccgccatggt cgagggcatc gccagcggcg atatcgacgt gatcgtgtcg
      361 gcccacgatc cccaggacgc cgatggcaag cgccgtccct tcgccgaagc agccgacggc
      421 gccatcggcg tcgagacgct gctctccgcc gcactccgcc tttatcacga cggcagcgtc
      481 gatctttcca ccttgcttcg ggccttgacc gcgaaccctg cgcgcctgct cggacttccg
      541 agcggccgcc tcgccaaggg agcgccggcc gacctcgtcc tcatcgatct cgacacgcct
      601 ttcgtggtga atcccgagct tttgcgtacg cggtcgaaga acacgccctt cgacgaggcg
      661 cggctgcaag gccgggcgct gatgacgctc gttgccggac gcgcggtgta caactacggt
      721 gagcatggca gttagtcttt ctcgttccgg ggtctgaccg atcatgctcg cggccgactg
      781 gagcctctac gatctcctcg cgctcggcct cggctatctg ctcggctcaa tcccctttgg
      841 cctcatcgtc accaagctcg ccggccatgg cgatctgcgc gacatcggct caggcaatat
      901 cggcgccacc aacgtactgc gcaccggcaa caagaggctc gccgcactca ctttgctgct
      961 cgatctgttg aagggcaccg ccgccgtgct catcggcgca cgcttcgggc ctgatgccgc
     1021 gatccttggc ggccttggcg ccttccttgg acatctcttc ccggtctggc tcggcttccg
     1081 cggcggcaag ggcgttgcga cctatatcgg cgttctgctc ggactgtatt ggccgggggc
     1141 gatcgccttc tgcgcgatct ggctgctggt cgcgctcgtc acccgctact catcgctctc
     1201 ggcgctcgcg gcgagtgccg caatccccct gatcctcgtc cttgtcggca acacacggat
     1261 cgctctgttg ttcctctttt tcaccgtgct gctctattgg cgccacgccg acaatatcgg
     1321 acgcctgctg cgaggcgagg aggggcgcat tggcgcgaaa aagacctgat cagggccgca
     1381 acatgctcgc tgcccgctca ggcgtccagc tgagcgaccg ccggcggctg agctggctca
     1441 ggctgatccg cagcgagaat gtcggcccgg caaccttccg cgcgctcgtc aatcaattcg
     1501 gcggcgccga ggcggcgctc gacgcgctgc ccgccttgtc ccgccgcggc ggccgcgcct
     1561 cgatccgggt tttcaccgag gctgaggccg aggccgagct tgaggctgcc gaccgcgccg
     1621 gtgccagttc gtggtccatg ggggagaagg gctatccgcc cgcgctctcc catgtcgata
     1681 cgccgccgcc gctcctctat gccaaaggca ggctcgacct tgccgacgct ccgatcgtcg
     1741 ccatggtcgg cgcccgcaac gggtctgccg tcggacagaa gctcacccgg cagcttgcca
     1801 ccgagctcgg gcttgaaggc ttcgtcattg cttcaggtct tgcccgcggc atcgacaccg
     1861 ccgcgcatct tgccgcgctc gagcggggca cgatcgccgt gctcgccggc ggcatcgaca
     1921 tcgtttatcc gccggagaat acagaccttc agcacgccat cggcgaacag gggctcctgc
     1981 tcagcgagcg cagcccaggc ttcgccccaa ggggcaagga cttccctcgc cgcaaccgcc
     2041 tgatctccgg catctcgatt ggggttgtgg tggtggaggc agccgaacgg tcaggctcgc
     2101 tgatcaccgc ccgctttgcc ggcgagcagg gccgcgaggt ctttgccgtg cccggtagcc
     2161 ccctcgaccc gcgctcagca ggcaccaaca atctcctcaa gcagggcgcc tgcctcgtga
     2221 cctcggcccg cgacatcgtc gaagctctgg cgccgattct cggccgcccc gtcgaaccgt
     2281 tcggccccga gctgtccgcc cctgaggaag gggtatcgcc gagcccctgc ccgacatcgt
     2341 gcaaagcgag cgggagcgga tcatcggcgc gctcgggccg agcccggtcg atatcgacga
     2401 ggtcgcccgg gccaccggca tcgcggtgcg caaggtgcat atcgttctgc tggagctcga
     2461 tctcgcggaa aagttgcaga gagacggaca acaacttgtg tcattgatcg gggaggacta
     2521 gccctcaatc gtcccgcgcg cgctcgactt gcgcctcttc cttggccatg gcgagcagat
     2581 aggcgatgag cggcagtccc gcggcctggg cgatcgactt cagctcatcg accagggtgg
     2641 cgatgtagtc cgcggcctcc cgccgcgtca tttcggatcc gatgatgggg ccttctcttt
     2701 cgggcgcttt cctggaccgg ggcgccccat tttgcttttt ctgacgccgg ttcgccaaac
     2761 gtgctctcct agtgtgttaa cagcttcagg ttgtgttaaa tagatatacc tcctggagtt
     2821 gtcaatctcg acttcctaga atgccgcact cctcattcag cggaaattga cactcggcgt
     2881 gcaagacacc attttcgcct caatgcgagg gcaagacgcg gcaaacctcc ccctcgggcc
     2941 gggcgccttc agtgccggct atggaaccac ttaatgaaca ttgtgatcgt tgagtcgccg
     3001 gctaaggcca agaccgtgaa caagtatttg gggtcgggct atcgcgtgct cgcctcctat
     3061 gggcacgtcc gcgacctgcc ctccaagaac ggctcggtcc tccctgacaa ggacttcgag
     3121 atgcattggg acgtcgagcc caaggctgcg aagcggctgg atgagatcgc caaggcggtc
     3181 aaaggcgcaa ccaagctcat cctcgccacc gaccccgacc gcgagggcga ggccatttct
     3241 tggcacgtct accagattct gaatcgcaag aaggcccttg ataaggttgc ggtcgagcgc
     3301 gtggtgttca acgccgtgac caaggaagcc gtgctcgacg ccatgcgcca tccgcgcgag
     3361 atcgacgggc cgctggtcaa cgcctatctc gcccgccggg cgctcgacta cctcgtcggc
     3421 ttcaccctat ctccggtgct gtggcgaaag ctgccgggcg cccgctctgc cggccgggtg
     3481 cagtcggtgg cgctccgtct cgtctgcgac cgcgaagccg agatcgaggc cttcaaggcc
     3541 caagaatatt ggtcgctcgc cgccgagctc gccaccctca agaacgaaac tctcctcgcc
     3601 cgcctcaccg ccatcgacgg caagaagctc gagaagttcg acattgccga cgaggccacc
     3661 gccatgcggc tcaaggctgc tctggaaggc ggcagcttca tcgtgcagag cgtggagagc
     3721 aaggctcagc gccgccaccc ggcgcccccg ttcaccacct cgaccctgca gcaggaggct
     3781 tcacgcaagc tcggcttctc tccgcgccac accatgcaga tcgctcagcg gctctatgaa
     3841 ggcgtggaca tgggtggcga aagcgaaggc ttgatcacct atatgcggac cgacggcgtg
     3901 cagattgcgc ccgaggccat cgccgcggcg aggcgcacgg tcgagcagct ctatgggcgg
     3961 aactacgtgc ccgcaagccc acgccaatac gagacaaagg ccaagaacgc gcaggaagcc
     4021 cacgaagcca tcaggccgac cgatttcggc aagccgcctg agaaggtcgc ccgctatctc
     4081 gatgacgagt ccctcaagct ctacaagctc gtgtggcagc ggacgcttgc gagccaggct
     4141 tcgagcgccg agatcgagcg caccacggcc gagatcggcg tcaccggcaa tgacggggtc
     4201 gcctacggct tgcgcgccac gggctcggtg atccgcttcg acggctttct caagctttat
     4261 gaggaaggca ccgacgatgt gatcggcgag gacggcgctc tccccattct agcccaaggc
     4321 gatcagctca agggccgcaa gatcgatgcc aagcagcatt tcaccgagcc gccgccccgc
     4381 tacagcgaag caagcctcat caagaagatg gaggagctcg gcatcggccg gccgtcgacc
     4441 tatgcctcga cgctcaacat cttgcgcgac cgcgaatatg tccggctcga caagaagcgg
     4501 ctccatcccg aggacaaggg gcgcctcgtt accgcgttcc tcgagaactt cttccgccgt
     4561 tacgtcgaat acgacttcac cgccgacctc gaggagaagc tcgacctgat ctcggccgga
     4621 cagctcgcct ggaaggacgt gcttcgcgac ttctggcgcg atttcatcgc cgccgtccac
     4681 gatatcggcg acttgcgcat cgccgaggtg ctcgatgcgc tgaacgacat gcttgggccg
     4741 catattttcc cgggcacgga agccggtggc gatccgcgcg cctgcccctc ctgcgagacc
     4801 ggccgcttga gcctcaagat cggcaagttc ggcgccttca tcggctgctc caactatccc
     4861 gactgccgc
//
LOCUS       DATNAE010000151         2348 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2251542, whole genome shotgun sequence.
ACCESSION   DATNAE010000151 DATNAE010000000
VERSION     DATNAE010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2251542"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1964
                     /locus_tag="VN518_03340"
     CDS             <1..1964
                     /locus_tag="VN518_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HXE03638.1"
                     /translation="ILRRHAELVDLKPSFTVLDTDDQIRLLKQLLDAENIDEKRWPAR
                     VLASMIDGWKNRGLTPKRVPEGESFAFANGKARELYALYQRRLKELNAADFGDLLLEN
                     LRLFQEHADVLAKYQQRFLYMLVDEYQDSNVAQYLWLRLLAQGNGNICCVGDDDQSIY
                     GWRGAEVDNILRFETDFPGAKIIRLERNYRSTGHILGAASGLIAHNKGRLGKTLRTDG
                     ELGGKVEIEGCWDDEEEARIIGEDIEQMQRRGHALSEIAILVRASFQMRAFEDRFITL
                     GLPYRVIGGPRFYERQEIKDATAYFEVTLNPANDLKFERIVNTPRRGLGESTLKSLHQ
                     LARAGGVPLFQAARLIVETEELKPKPRKALADLIAAFDRWRSMIETMRHTELAELILD
                     ESGYTGMWQSERSPQAQSKLENLKELIRFMEEFDTLAGFLEHVSLVMDAQTEEGAERV
                     SLMTLHSAKGLEFDTVFLPGWDEGLLPHQRSLDDAGRSGLEEERRLAHVGLTRARKRA
                     KITFAQNRRTRGTWQSALPSRFIDELPIANVEVAEDKGQRGYGPSRFETAETVFASGF
                     YETPGWQRAQRNRPQGMTAPPNRGPITIDGTLIASSTAEGAGLTVGLRVFHQKFGYGR
                     ILHVDGNKLTVDFDKAGEKKVVDSFVERV"
     gene            1964..>2348
                     /locus_tag="VN518_03345"
     CDS             1964..>2348
                     /locus_tag="VN518_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03639.1"
                     /translation="MRPSVLLAVATACVASSAGAAPLWTVEKSEIAYLFYGEPGRPVL
                     SISCGQQEGEAGKDATRIEVEVETGTKPGKDDVVLRIEQDKGHKDVPLTPLICGGLSE
                     CPNQPDGEVYRYEASVPGKSLALDIA"
BASE COUNT          446 a          751 c          778 g          373 t
ORIGIN      
        1 agatcctccg ccgccatgcc gagctggtcg atctgaagcc gtctttcacc gtgctcgata
       61 ccgacgatca gatcaggctg ttgaagcaac tgctggatgc cgagaatatc gacgagaagc
      121 gctggccggc gcgggtactg gcgtcgatga tcgacggctg gaagaaccgc gggctgacgc
      181 ccaagcgcgt gccggaaggc gagagcttcg ccttcgccaa cggcaaagcg cgcgagctct
      241 atgcgctcta tcagcggcgc ctgaaggagc tcaacgccgc cgatttcggc gatctcttgc
      301 tggagaatct gcggctgttc caggagcacg ccgacgtgct ggcgaaatac cagcagcgct
      361 tcctctatat gctggtggac gagtatcagg actccaacgt cgcgcaatat ctgtggctgc
      421 ggctgctggc gcagggcaac ggcaatattt gctgcgtcgg cgacgacgac cagtcgatct
      481 atggctggcg cggcgccgag gtggacaaca tcttgcgctt cgaaaccgac tttcccggcg
      541 ccaagattat ccggctcgaa cgcaactacc gttccaccgg gcatatcctc ggcgcggcct
      601 ccggcctgat cgcgcataac aaaggccgcc tcggcaagac gctgcgcacc gacggcgagc
      661 tgggcggcaa ggtcgagatc gagggctgct gggacgacga ggaggaggcc cgcatcatcg
      721 gcgaggacat cgagcaaatg cagcgccggg gccacgcctt gagcgagatc gccatcctgg
      781 tgcgcgcgtc gttccagatg cgcgccttcg aagatcgctt catcacgctc gggctgccct
      841 atcgcgtgat cggcggcccg cgcttctacg aacggcagga gatcaaggac gcgaccgcct
      901 atttcgaagt gacgctcaac ccggccaacg atctcaaatt cgagcgcatc gtcaatacgc
      961 cgcggcgggg gctcggcgag tcgacgctga aatcgctgca tcaactcgcc cgcgccggcg
     1021 gcgtgccgct gttccaggcg gcgcggctga tcgtcgagac cgaggagctg aagccgaagc
     1081 cgcgcaaggc gctcgccgat ctcatcgccg ccttcgaccg ctggcgctcg atgatcgaga
     1141 ccatgcggca taccgagctc gccgagctga tcctcgatga gtcgggctat accgggatgt
     1201 ggcaatccga gcgttcgccg caggcgcagt cgaagctcga gaacctgaaa gagctgatcc
     1261 gcttcatgga agagttcgac acgctcgccg gtttcctcga gcatgtgtcg ctggtgatgg
     1321 atgcgcagac ggaagaaggc gccgagcgcg tcagcctaat gacgctgcat tcggccaagg
     1381 ggctcgaatt cgacacggtg ttcctgcccg gatgggacga agggctcctc ccccatcagc
     1441 gcagccttga cgatgcggga cgctctgggc tcgaagagga gcggcggctt gcccatgtcg
     1501 ggctcacgcg ggcgcgcaag cgcgccaaga tcaccttcgc gcagaaccgc cgcacccgcg
     1561 gcacctggca gagcgcgctg ccatcgcgct tcatcgacga gctgcctatc gccaatgtcg
     1621 aggtcgccga ggataagggc cagcgcggct acgggccgag ccgcttcgag acggccgaga
     1681 ccgtgttcgc cagcggcttc tacgagacgc cgggctggca gcgcgcgcag cgcaaccggc
     1741 cgcaagggat gaccgcgccg cccaatcgcg gaccgatcac catcgacggc acgctgatcg
     1801 cgtccagcac ggccgagggc gccgggctta cggttggctt gcgcgtgttc catcagaaat
     1861 tcggctatgg ccgcatcctg catgtcgacg gcaacaagct caccgtggat ttcgacaagg
     1921 ccggagagaa gaaagtggtc gacagcttcg tcgagcgggt gtgatgcggc cgtctgtgct
     1981 gcttgccgtc gcgacagctt gcgtggcgtc gagtgccggc gcggcaccgc tgtggaccgt
     2041 cgagaaatcc gagatcgcct atctgttcta cggcgagcct ggccggccgg tgctgtccat
     2101 ctcttgcgga cagcaggagg gcgaggccgg caaggacgca acccgcatcg aggtcgaggt
     2161 cgagaccggc accaagcccg gcaaagacga cgtggtgctc cgcatcgagc aggacaaagg
     2221 ccacaaggac gtgccgctca cgccgctgat ctgcggggga ttgagcgaat gcccgaacca
     2281 gcccgacggc gaggtctatc gctacgaagc gagcgtccca ggcaagagcc ttgcactcga
     2341 catcgccg
//
LOCUS       DATNAE010000152         2438 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2555760, whole genome shotgun sequence.
ACCESSION   DATNAE010000152 DATNAE010000000
VERSION     DATNAE010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2555760"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="VN518_03350"
     CDS             complement(<1..869)
                     /locus_tag="VN518_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009467859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXE03640.1"
                     /translation="MLAALSIRDIVLIDKLDLEFGQGLSALTGETGAGKSILLDAFSL
                     AIGGRGDASLVRQGQAQGQVTAIFELPKSHPVFALLAENGIEAEEGLILRRVQGADGR
                     SRAFINDIGVSIQLLRLVGQALVEIHGQHDDRALIDPSGHRELVDAFGGLSGEAARVA
                     AAYATWQQADAERARHEAEIAATRANADYVSHALDELRSLDPQAGEEESLAERRQLMM
                     HAEKLAGELSEALDALAGEGTSGARLAAALRRIERQASVAPLLLTPVAEALERVLTET
                     DTARAKIEEAIAL"
     gene            complement(883..1755)
                     /locus_tag="VN518_03355"
     CDS             complement(883..1755)
                     /locus_tag="VN518_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HXE03641.1"
                     /translation="MSALEKPFGAPGVRPAALSFLGVILLAGLLGGCGSLFNGDKNVV
                     EDPNEPPDVIYKKAETLADKGKFGDAAKQYENVDINHPYSQEARRAILMAAFAYYKAG
                     KYDDAIGAADRYLTLHPGTPEADLAQDIIAMSYYDRVLDPRRDQTNARKALEAYETLI
                     QRYPESRYAAEARNRTRIMRDLLAAQEMMVGRYYLRHNNFLAAINRFRTVVTDYQTTE
                     QVEEALMRLTEAYMALGIVNEAQTAAAVLGHNFPDSKWYSHAYALLGNVGLQPQQHEG
                     SWITQTWKGNGNPA"
     gene            complement(1975..>2438)
                     /locus_tag="VN518_03360"
     CDS             complement(1975..>2438)
                     /locus_tag="VN518_03360"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HXE03642.1"
                     /translation="EIDFDSPLIGSQRISLDINPGSFRRELSRARTFGFMKDVERLWA
                     AGLALGASLENTVAIGDDRVINREGLRFTDEFVRHKALDAVGDLALAGAPILGSFRSY
                     RGGHRLNALVLKALFADPEAWTMIEAPRYREIRHAELSHRLAAANFAAERS"
BASE COUNT          414 a          770 c          827 g          427 t
ORIGIN      
        1 ctgagcgcga tcgcctcctc gatcttggcg cgcgccgtgt cggtctcggt cagcacgcgc
       61 tcgagcgcct cggcgacagg ggtgagcagg agcggtgcga ccgaggcctg gcgctcgatc
      121 cggcgcagcg ccgcggcgag ccgcgcgccg ctggtacctt ccccggcgag cgcatcgagg
      181 gcctctgaaa gctcgccggc aagcttctcg gcatgcatca tcaattgccg gcgctcggcc
      241 agactttcct cttcgccggc ttgcggatcg aggctgcgca gctcatccag cgcatggctc
      301 acatagtcgg cgttggcgcg ggtggcggcg atctcggcct catggcgcgc ccgctcggcg
      361 tcagcctgct gccacgtggc ataggcagcg gcgacgcgcg ccgcctcgcc gctcaacccg
      421 ccaaaggcgt ccaccagctc gcgatggccc gacgggtcga tcagcgcccg atcgtcatgc
      481 tggccgtgga tctcgaccag agcctgcccg acgaggcgca acagctggat actcacgccg
      541 atatcgttga tgaaggcccg gctcctgcca tcggcgccct gcacgcggcg caagatcagc
      601 ccctcctcgg cctcgatgcc gttctcggcc agcagcgcaa acaccggatg gctcttcggc
      661 agctcgaaaa tcgccgtgac ctggccttgg gcttggccct ggcgcacgag cgaggcatcg
      721 cctctgccgc cgatggcgag cgagaaggca tcgagcaaga tcgatttgcc ggcgccggtc
      781 tccccggtaa gcgccgacag gccttggccg aattcgagat cgagcttgtc gatcaggacg
      841 atgtcgcgga tcgacagggc ggctagcatg gcgcgaagcg ggtcaggcgg ggttgccgtt
      901 gcccttccag gtctgggtga tccaagagcc ttcgtgctgt tgcggctgga ggccgacatt
      961 tcccaggagc gcgtaagcgt ggctgtacca cttgctgtcc ggaaagttgt ggccaagcac
     1021 ggcggctgcc gtttgagcct cgttgacgat gcctaacgcc atataggcct cggtcaaacg
     1081 catcaaggcc tcttccacct gctcggtggt ttggtagtcg gtgacgacgg tgcggaagcg
     1141 gttgatggcg gctaggaagt tgttgtgacg gagataatag cggccgacca tcatctcctg
     1201 tgccgcgagg agatcgcgca tgatgcgcgt ccggttgcgc gcctcggcgg cgtagcgcga
     1261 ctcgggatag cgctgaataa gcgtctcgta ggcctcgagc gccttgcgcg cattggtctg
     1321 atcgcgcctc gggtcgagga cgcggtcgta ataggacatg gcgatgatgt cctgggcgag
     1381 gtccgcctcc ggggttccag gatggagcgt gaggtagcga tccgcggcgc cgatcgcgtc
     1441 gtcgtatttg ccggctttgt aataggcgaa agccgccatg aggatggcgc ggcgcgcctc
     1501 ctgcgaataa ggatgattga tatcgacatt ttcgtattgc ttggccgcgt cgccgaattt
     1561 gcccttgtcg gccagcgttt cggccttctt gtagatgacg tccggaggct cgttgggatc
     1621 ctcgactacg ttcttgtcgc cgttgaagag cgagccgcag ccgccaagca gtcccgcgag
     1681 gaggatgacg ccaaggaagc tcaaagccgc cggccggacg ccgggcgctc caaatggctt
     1741 ctccagcgct gacatgctcc ccctcaaaga tcgtccctcg cgccagaggg ccagtttcgc
     1801 aagccgccgc gtgcttgcca aggctggcgt ggcaaccagc aaacaacgcg ctgattatgg
     1861 tgtttttttg ccctgttccc tgaccgaagc cgcggagtca ggccgcgcga cattgaggcg
     1921 ccaaaacgcg ccgacagcgc aaatcgaggc tagagcagcg agtgcggctt cccgttagct
     1981 ccgctcggcg gcgaaattcg cggccgcaag gcggtggctg agctcggcat gacggatctc
     2041 gcggtaacgc ggagcctcga tcatggtcca agcctcggga tcggcaaaca gggccttcag
     2101 caccagcgcg ttcagccgat ggccgccgcg atacgagcgg aaggagccga ggatgggcgc
     2161 gccggcgaga gccagatcgc cgaccgcgtc gagcgccttg tggcgcacga actcgtcggt
     2221 gaagcgaagc ccctcgcggt tgatgacgcg atcatcgccg atggcgacag tgttctcaag
     2281 cgaagcgcca agggcgagac cagcagccca gagccgctcc acgtccttca tgaagccgaa
     2341 agtgcgggcg cgggagagct cgcggcggaa tgagcccggg ttgatgtcca gcgagatgcg
     2401 ctgcgagccg atcagcggcg agtcgaaatc gatctcga
//
LOCUS       DATNAE010000153         8365 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_1952616, whole genome shotgun sequence.
ACCESSION   DATNAE010000153 DATNAE010000000
VERSION     DATNAE010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 8365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8365
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1952616"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1276
                     /gene="groL"
                     /locus_tag="VN518_03365"
     CDS             <1..1276
                     /gene="groL"
                     /locus_tag="VN518_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509708.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HXE03643.1"
                     /translation="DMAVTAVLDELKAKAKKVTSSEEIAQVGTISANGDHEIGAMIAK
                     AMQKVGNDGVITVEEAKSLETELEVVEGMQFDRGYISPYFITDADKMRVELESPYILI
                     NEKKLSNMQSLLPLLEQVVQAGKPILVIAEDVEGEALATLVVNKLRGGLKVAAVKAPG
                     FGDRRKAMLQDIAVLTNGQVISEDLGIKLENVTLDMLGRAKKVVITKDDTTIVGGAGK
                     KSEIEARVAQIRQQIEDTTSDYDREKLQERLAKLAGGVAVIKVGGVTEMEVKEKKDRV
                     DDALNATRAAVEEGILPGGGVALIRSQSVLAKLKAANDDQRVGINIVRRALHAPARQI
                     AENAGEDGAVVVGKIVDKDDYAFGYNAATGEYGNLVKQGVIDPAKVVRTALQDAASVA
                     GLLITTEAMVAEKPKKKAPAMPQGGGMGDMDF"
     gene            1477..2292
                     /locus_tag="VN518_03370"
     CDS             1477..2292
                     /locus_tag="VN518_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002712422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HXE03644.1"
                     /translation="MAKKPTTPSGRIRVGIGGWSYAPWRGVFYPKGLKQADELSYATS
                     KLTSIEINATHYRLQSPKSFRIWGATAPDGFVFSVKGPRLITQQKVLAETGNFITRFF
                     KSGLAELGDRLGPILWQFAPFKRFDEDDFAKFLDLLPHELDGQELNQVVEVRHASFAI
                     PRFAELARDAGVTIAYVDAEGWPNIADLTGDVIYARLQRGDDALAAAYPPGELDSWAG
                     RAKTWAKGNPPSDLKLLAESAASKPRPRDVFIYFIHEGKLRAPAAAMALIERL"
     gene            complement(2289..3470)
                     /locus_tag="VN518_03375"
     CDS             complement(2289..3470)
                     /locus_tag="VN518_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HXE03645.1"
                     /translation="MFAALSASGIDTRVVGGAVRNALLGLPVIEAVDLATTAEPERVM
                     ALAEEAGLKAVPTGIDHGTVTVIADGLPFEVTTLRRDVETFGRHAKVAYTTSWEEDAK
                     RRDFTLNALYADRDGKVFDPLGGYDDLAAGRVRFIGDAEARIKEDFLRILRFFRFHAY
                     YGKGDMDAAGLKAAVKLRAGLEQLSAERVAGELRRILVAPQAARAVDALYDYGLLTGV
                     LGGVPRLGRFERLVAIGAANALAPDASLRLAALAVFVEEDVARLAERLRLSNAEQAVL
                     ALAAEKGLVAGLPEETAAKSALYRLGPSYRSALLLAWLDSDASPDDPEWRKALALPVR
                     WQAPSFPIGGNDVMTFGELKGPEIGELLKVLEQDWIASGFALGRDELLARALGLIAAR
                     G"
     gene            complement(3562..3822)
                     /locus_tag="VN518_03380"
     CDS             complement(3562..3822)
                     /locus_tag="VN518_03380"
                     /inference="COORDINATES: protein motif:HMM:NF040327.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6111 family protein"
                     /protein_id="HXE03646.1"
                     /translation="MLRIALIDIFMFSLPFLIYGAYMLAAKGAAPTDVWQDAPVLWLL
                     AAGCGLLLITMATLIQFSGGKPGGTYHPATTEGGVIKPGEID"
     gene            complement(3910..4473)
                     /locus_tag="VN518_03385"
     CDS             complement(3910..4473)
                     /locus_tag="VN518_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1285 domain-containing protein"
                     /protein_id="HXE03647.1"
                     /translation="MARRDEKSVLRALGETQAKSLPAGRRVPQDCGDIGLKIGRDGTW
                     YYRDSAIGRKPLVKLFASVLRQERDGRYYLVTPAEKVLIHVEDSPFLAVGMTVEGSGK
                     RQRLSFRTNLDDEVKAGPDHPLSFRPEKNGSFTPFVLVRDKLKARLTRPVYYELVAAA
                     ATEGKGAKRGLGVWSGGTFFPFPGRKP"
     gene            4640..5638
                     /locus_tag="VN518_03390"
     CDS             4640..5638
                     /locus_tag="VN518_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HXE03648.1"
                     /translation="MTTLASTETQLLELIDEIGAKIHDVREATATTIFGQERVIDLAL
                     ITLLSGGHALLIGVPGLAKTSLVETLGAVLGLQNKRIQFTPDLMPSDIVGSEVLEEGT
                     GGRRSFRFVKGPVFTQLLMADEINRASPKTQSALLQAMQEHHVTVAGVRHDVPRPFHV
                     LATQNPLEQEGTYPLPEAQLDRFLLQIDVSYPDEAAERRMLFATTGLEERSLKTILSG
                     DELMMAQRLVRQLPVGDQVVDAILKLVRSARPGTGIDQEFEDMIAWGPGPRASQALML
                     AVRAKAMIDGRLAPSIDDVIELAEPVLKHRMALTFAARAEGLQMSDLVAKLVKPLG"
     gene            5646..6602
                     /locus_tag="VN518_03395"
     CDS             5646..6602
                     /locus_tag="VN518_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HXE03649.1"
                     /translation="MAKREAKALHPFPAEEPEAHALAARMPALLIEAQRVAHTVTHGS
                     HGRRRAGPGETFWQFRHFDYNDSRSGIDWRRSGSSDHLFVREREWEAAHTVWLWVDLS
                     PSMQFRSRLSNTTKESRAVVLSLALSELLARAGERVGVLGSRPYTGRSAARRVAEVLV
                     GDAGKATSLPPPARLSRFSECLLFSDFLEPVEETAKRVEAIAAQGVRGHLVQILDPAE
                     ETLPYEGRTEFSASEGRDRVIAGRAENLRERYRERLISHRNALVAEAKRFSWSFLLHH
                     TDRPAEEVVLAIHNRLAGLERDYRFRASTPFAAGATGARSQS"
     gene            6599..>8365
                     /locus_tag="VN518_03400"
     CDS             6599..>8365
                     /locus_tag="VN518_03400"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HXE03650.1"
                     /translation="MMSFGAIGFLQPWMLLGLAALPAIWWLLRLTPPTPQHVVFPPTR
                     LLKNLKSTEETPAHSPWWLTALRMLLAALIVLALARPVLNPDRESFAGTGPLLVVVDN
                     GWASAAHWPERREVINAAIDRAMRDGRTVVLAPSAPGDPLIGPIAAEQARQRAAGLAP
                     QPYAPDRKALAETLQRELGGRTDYSVVWLSDGLDYGEGRSFADSLAKLAGSAGSVLML
                     RPDRDDAPLALDQTAGERSGLTARILSGAEGPRAGVVRAVTGRGEPLGEVSFTLPRGA
                     RDTKAQFDLPLEIRNQVARIEIGGERSAGAVYLLDARSEWHRVGIISGESREAAQPLL
                     SPLYYVQRALSPYADVVTPAEGNVSTGVHELIDDKVSTVVLADIGKLVPGTEEELERW
                     LKAGGVLIRFAGPRLEQGGDELLPVALRRGGRSLGGSLSWSTPQPLAAFEPSSPFRGL
                     ALPDDVKVNRQVLADPTVATESQVWARLADGTPLVTASRNGEGWIVLFHVTANSDWSN
                     LPLSGLFVEMLRRTVNMGPSKVDVSSGDQAQAATAGAKSASPNLVSASALPPIQTLDG
                     YGQLGSPPARAAPIASDQVDTAQ"
BASE COUNT         1493 a         2826 c         2621 g         1425 t
ORIGIN      
        1 cgacatggca gtaactgccg ttctcgacga actgaaggcc aaggccaaga aggtcacctc
       61 cagcgaagag atcgcgcaag tcgggaccat ctcagccaat ggcgaccacg agatcggcgc
      121 tatgatcgcc aaggccatgc agaaggtcgg caatgacggc gtgatcaccg tcgaggaggc
      181 gaagtcgctc gagaccgagc tcgaagtggt cgagggcatg cagttcgacc gcggctatat
      241 ttccccctac ttcatcaccg acgccgacaa gatgcgcgtc gagctcgaga gcccctacat
      301 tctcatcaac gagaagaagc tctccaacat gcagtcgctg cttcccctgc tcgagcaggt
      361 ggtgcaggcc ggcaagccga tcctagtcat tgccgaggac gtcgagggcg aggctctcgc
      421 cacgctcgtg gtgaacaagc ttcgcggtgg gctgaaggtc gcggccgtca aggccccagg
      481 ctttggcgat cgccgcaagg ccatgctgca ggacatcgct gtgctcacca acggtcaggt
      541 gatctccgaa gatctgggca tcaaactcga gaacgtcact ctcgacatgc taggccgcgc
      601 caagaaggtg gtcatcacca aggatgacac gaccatcgtc ggcggtgccg gcaagaagtc
      661 cgagatcgag gcgcgcgtcg cccagatcag gcagcagatc gaggacacta cgtccgatta
      721 cgatcgcgag aagctccagg agcgtctggc caagctcgcc ggcggcgtcg ccgtgatcaa
      781 ggttggcggc gtcaccgaga tggaggtcaa ggagaagaag gaccgcgtcg acgatgctct
      841 gaatgcgacg cgggccgcgg tcgaggaagg cattctgccc ggcggcggtg tggctctgat
      901 ccgctcgcaa tccgtcctcg ccaaactgaa ggccgccaat gacgaccagc gggtcggcat
      961 caatatcgtc cgacgcgcgc tccacgcgcc ggcccgacag atcgccgaga atgccggcga
     1021 agacggcgcc gtggtcgtcg gcaagatcgt ggacaaggac gactatgcct tcggctacaa
     1081 cgccgcgacc ggcgaatacg gcaatctggt gaagcagggc gtcatcgacc cggccaaggt
     1141 cgtgcgcacc gcgctgcagg acgccgcctc cgtggcgggt ctcctcatca ccaccgaggc
     1201 gatggtcgcc gagaagccga agaagaaggc tccggccatg cctcagggtg gcggcatggg
     1261 cgacatggac ttctagtcag tcccgttgcg tcaagcgtca acgaagggcc gtgtccaaag
     1321 ggcgcggccc ttttcttttg ctttgcctgg cctaccagat cgctcattgt tattgcccgg
     1381 tttgaccgcg caatccaatg ctcccggtga agcgaacaat cgaacaccct tgattactgg
     1441 atgcctggct tcgcgggcat gacgaggaag attctcatgg ccaagaaacc cacgacacct
     1501 tcaggccgca tccgcgtcgg catcggcggc tggagctacg cgccgtggcg cggggtgttt
     1561 tatccgaagg gcctgaagca ggctgacgag ctcagctacg ccacgagcaa gctcacctcg
     1621 atcgagatca acgccaccca ttatcgcctg caaagcccga aaagcttccg catttggggg
     1681 gcgaccgcgc ctgatggttt tgtgttctcg gtcaaaggcc cgcgccttat cacccagcag
     1741 aaggtgctcg ccgaaaccgg caacttcatc acccgctttt tcaagtctgg gctggcagag
     1801 ctcggcgaca ggcttgggcc aatcctctgg cagttcgcac ccttcaagcg cttcgacgag
     1861 gacgatttcg caaagtttct ggacctgctt ccgcacgagc tcgatggaca ggagcttaac
     1921 caggtggtcg aggttcgcca cgcgagcttc gccattccgc gtttcgccga gctcgcgcgc
     1981 gacgcgggcg ttaccattgc ctatgtcgac gccgagggct ggccgaacat cgccgacctc
     2041 accggagatg ttatctatgc gcggctgcaa agaggcgacg acgcgcttgc ggctgcctat
     2101 ccccctggcg agctcgactc ctgggccggc cgcgccaaga cctgggcgaa aggcaaccct
     2161 cccagcgacc tgaagctgct tgcggagtcc gcagcatcga agcctcgccc gcgcgacgtg
     2221 ttcatctact tcatccacga aggcaaactg cgcgctccgg ccgcagccat ggccctgatc
     2281 gagcggctct agccccgcgc ggcgatgagc cccagcgccc tggcgagaag ttcatcgcgg
     2341 ccgagcgcaa agccggaggc gatccaatcc tgctcaagca ccttcagcag ttctccgatc
     2401 tcagggcctt tgagctcgcc aaacgtcatg acgtcgttgc cgccgattgg gaaggatgga
     2461 gcctgccatc gcacgggcag cgccaatgcc ttgcgccatt ctggatcatc gggagaggcg
     2521 tcggagtcta gccaggcaag cagcagagcg gagcggtagc tcgggccgag ccgatagagc
     2581 gctgatttcg ccgcggtctc ctcgggcaag cccgcgacga ggcctttctc tgccgcaagc
     2641 gccagcaccg cctgctcggc gttggaaagc cgcaaccgtt cggcaaggcg cgccacatcc
     2701 tcctcgacga agaccgccaa cgccgcgagc ctcaggctag cgtcaggcgc caatgcgttg
     2761 gccgccccga tggcaaccag tctctcgaac cggccaagcc gcggcacgcc gccaagcacg
     2821 ccggtgagaa ggccataatc gtaaagcgcg tcgaccgcgc gcgcggcttg gggtgccact
     2881 aggatgcgcc gcaactcgcc ggcgacccgc tcggcggaga gctgctcgag cccggcgcgc
     2941 agcttcaccg cagccttgag gccggcggcg tccatgtcgc ccttcccgta ataagcgtgg
     3001 aagcggaaga agcggaggat gcgcaaaaag tcttccttga tgcgcgcctc ggcgtcgcca
     3061 atgaagcgca ccctccccgc agcgagatcg tcatagccgc cgagcggatc gaataccttg
     3121 ccgtcgcgat cagcgtaaag cgcattgagg gtgaagtcgc ggcgcttcgc gtcctcctcc
     3181 catgaggtgg tgtaggcgac cttggcgtgg cggccgaagg tctcgacgtc acgcctgagc
     3241 gtggtgacct cgaacggcaa gccgtcggca atgacggtga ccgtgccgtg atcgatcccg
     3301 gtcggcacgg ccttgagccc tgcctcttcg gcaagcgcca tcaccctctc aggctcagcc
     3361 gtggtggcga gatcgacagc ctctatgaca gggagcccaa gaagcgcgtt gcgtacggct
     3421 ccgccgacca cgcgtgtgtc tatgccgctg gcgctgaggg cagcaaacac ggcgctcgtt
     3481 tcccctcgct tcagccactc tgcgcctgca agcgatggag cctcggagcg gcactcactg
     3541 tcatgcgcga tcatggggcc gtcagtcgat ctcgcccggc ttgatgaccc caccctcggt
     3601 ggtcgccgga tgataggtgc caccgggctt gccacccgaa aactggatca acgtcgccat
     3661 ggtgatcagc aaaagtccgc accccgccgc gagcagccag agcaccggcg cgtcctgcca
     3721 cacatccgtc ggcgccgcgc ccttggcggc aagcatgtag gcgccataga tcagaaatgg
     3781 cagggagaac atgaagatgt cgatgagggc gatgcgaagc atcgggaatc atgccttgct
     3841 caggaacgga cccgcacaat agcggcggag cccttataaa gcgagcggcg ctcgtgtcgc
     3901 tgaagcggcc tagggcttgc gccccgggaa ggggaagaac gttcctccac tccacactcc
     3961 gagcccgcgc tttgcgcctt tgccttcggt cgctgcagcc gccaccagct cataataaac
     4021 aggccgcgta agccgtgctt tcaacttgtc gcgcaccagc acgaagggcg tgaagcttcc
     4081 gttcttctcg gggcgaaagc tcaagggatg gtctggcccg gcctttacct cgtcgtcgag
     4141 attggtgcgg aagctgagcc gttgccgctt gccgcttccc tcgacggtca tgcccaccgc
     4201 aagaaagggg ctgtcctcga cgtgaatcaa aaccttctcg gccggcgtca cgagataata
     4261 gcggccgtcc ctttcctgcc tcagcaccga ggcaaagagc ttgaccaggg gtttccgccc
     4321 gatggcgcta tcccgatagt accaagtccc gtcgcgccca atcttaaggc cgatgtctcc
     4381 acagtcctgc gggacccggc gccctgccgg cagggatttg gcttgggtct cgccgagggc
     4441 acgcagcacg ctcttttcgt ctcgtcttgc catcttgagc gggccttgga ctcctcttac
     4501 ctaaccgtca acccggcgta ggagacgccg gcggagacga aacactattg tgaccaattt
     4561 ttaacccaaa gtattcctct ccccacggcc tttcggccta tagtccccct tcctgaccat
     4621 ttcgagccaa agcgagccca tgaccacact tgcttccacg gaaactcagc ttctcgagct
     4681 gatcgacgag atcggagcca aaatccacga cgtgcgcgag gcgacagcca ccaccatttt
     4741 tggccaggaa cgcgtcatcg accttgcgct gatcacgctc ctgtccggcg gccatgccct
     4801 cctcatcggc gttcccggtc tcgccaagac gagcctcgtg gaaacgctcg gcgcggtgct
     4861 cgggctccag aacaagcgca ttcaattcac cccggacctg atgccgtccg acattgttgg
     4921 gtcggaggtg ctggaggaag gcaccggcgg ccgccgcagc ttccgcttcg tcaaggggcc
     4981 ggtcttcacc cagctcttga tggccgacga gatcaaccga gcgagtccga agacgcagtc
     5041 agcactgctg caagccatgc aggagcatca cgtcacggtg gccggcgttc gccacgacgt
     5101 gccgcggccc ttccacgtcc ttgccaccca gaacccgctc gagcaggagg gaacctatcc
     5161 cctccccgaa gcccagctcg accgcttcct gttgcagatc gacgtctcct atcccgacga
     5221 ggcggctgag cggcgcatgc tgttcgccac cacgggactg gaggaacgtt cgctgaagac
     5281 catcctttcg ggcgacgagc tgatgatggc gcagcgcctg gtgcgccagc tcccggtggg
     5341 cgatcaggtg gtcgacgcca ttctcaagct cgttcgctcg gcgcgccccg gcaccggcat
     5401 cgatcaggaa ttcgaggaca tgatcgcctg ggggcccggc ccccgcgcca gccaagctct
     5461 gatgctcgcg gtccgcgcca aggcgatgat cgacgggcgg ctcgcccctt ccattgacga
     5521 cgtgatcgag ctggccgagc ccgtgctcaa gcaccgcatg gcgcttacct tcgccgcccg
     5581 cgccgagggt ctgcagatga gcgacctcgt ggcgaagctc gtcaagccgc tggggtgatc
     5641 ggagcttggc caaacgcgag gcgaaagcac ttcatccgtt tccggccgag gagccggagg
     5701 cccacgcgct agctgcgcgc atgccggcgc tgctcatcga ggctcagcgc gtcgcccaca
     5761 ccgtcaccca tggcagtcac ggccgcaggc gcgccggacc cggcgagacc ttctggcagt
     5821 tccgccattt cgactacaac gattcccgct caggcatcga ttggcggcga tcgggaagct
     5881 cagaccatct cttcgtgcgc gagcgcgaat gggaggccgc gcacacggtc tggctctggg
     5941 tcgatctctc gccgtcgatg cagtttcgct cgcggctgtc gaacaccacc aaggaaagcc
     6001 gcgccgtggt gctgtcgctt gcgcttagcg agcttcttgc ccgtgccggc gagcgcgtcg
     6061 gcgtgctcgg cagcaggcct tacaccggcc gcagcgccgc gcgccgggtc gccgaggttc
     6121 tggtcggcga tgccggcaag gcgacgagcc tgccgccgcc ggcaagactg agccgtttct
     6181 ccgaatgcct tctgttcagc gacttcctcg agcccgttga ggaaacggcc aagcgtgtcg
     6241 aagcaatcgc ggcgcagggc gtgcgcggcc atctcgtgca aatcctcgat cccgccgagg
     6301 agacactgcc ctacgagggc cgcaccgaat tctccgcaag tgaaggccgg gaccgcgtga
     6361 tcgccggacg cgccgagaat ttgcgcgaac gctatcggga gcggctgata agccaccgca
     6421 acgcgcttgt cgccgaggca aagcgcttca gctggtcgtt ccttctgcac cataccgaca
     6481 ggccggccga ggaggtggtg ctcgccatcc acaatcgcct cgctggcctt gagcgcgatt
     6541 atcgcttccg tgcgtcgaca cctttcgctg cgggcgcaac cggggcgagg tcgcaatcat
     6601 gatgagcttc ggcgccattg gcttcctcca gccctggatg ctgctcgggc tcgcggctct
     6661 tcccgcgatc tggtggctgt tgcggctgac accgccgacg ccgcagcatg tggtgttccc
     6721 gccgacgcgg ctgttgaaga acttgaaatc gaccgaggag accccggcgc acagcccctg
     6781 gtggctcacg gcgcttcgca tgttgcttgc cgccctgatc gtgctcgcgc tcgcccgtcc
     6841 ggtgctcaat cccgaccgcg agagctttgc cggaaccggc ccgctgttgg tcgttgtcga
     6901 caatggctgg gcttctgccg cccattggcc tgagaggcgc gaggtgatca acgcggcaat
     6961 cgaccgtgcc atgcgcgacg gcaggaccgt ggtgctggcg ccgtccgcgc ccggcgaccc
     7021 gctcatcggg ccgatcgccg ccgagcaggc gcgccagcgc gccgccggac ttgcgccgca
     7081 gccctacgcg cctgaccgca aggcgcttgc cgagacactt cagcgcgagc ttggcggcag
     7141 gacggactac agcgtcgtgt ggctttccga tggtctcgac tacggggaag gccggtcctt
     7201 cgccgacagc ctggccaagc ttgccggctc ggccggcagc gtcttgatgc tccgccctga
     7261 ccgggacgat gcgccgcttg cgctcgatca gaccgccggc gagcgaagcg gcctcaccgc
     7321 caggatcttg agcggagccg aaggcccgcg cgccggcgtg gttagagccg tcaccggccg
     7381 cggcgagccg ctcggcgaag tgtccttcac gctgccgcgt ggagcgcgcg ataccaaggc
     7441 gcaattcgat ctgccgcttg agatccgcaa ccaggtcgcc cgcatcgaga ttggcggcga
     7501 gcgctccgcc ggcgccgtct atctgctcga tgcgcgctct gagtggcacc gggtcgggat
     7561 catctccggc gaatcccggg aggccgcgca gcccttgctc tctccgctct attatgtgca
     7621 gcgcgcgctt tcgccctacg ccgacgtggt gacgccggcc gagggaaatg tctccaccgg
     7681 cgtgcatgaa ctgatcgacg acaaggtctc gaccgtcgtg ctcgccgata tcggcaaact
     7741 cgttccggga accgaagaag agctcgagcg ctggctcaaa gccggcggcg tgctcatccg
     7801 ctttgccggc ccgcgtcttg agcaaggcgg cgatgagctt ttgcctgtcg cgcttcgccg
     7861 cggcggccgg tctcttggcg gatcgttgtc ctggagcacg ccccagccgc tggccgcctt
     7921 tgaaccttcc agcccattcc gcgggcttgc attgccggac gacgtcaagg tgaaccgcca
     7981 ggtgctggcc gatcccaccg tcgccaccga gtcgcaggtg tgggcgaggc tcgccgacgg
     8041 cacgcccctc gtcaccgcat cgcgtaacgg cgaaggttgg atcgtgctgt tccacgtcac
     8101 cgctaattcc gattggtcga acctgccgct gtcgggcctg ttcgtcgaaa tgcttcgccg
     8161 caccgtcaac atgggcccga gcaaggtcga tgtgagctca ggcgatcaag cgcaggccgc
     8221 gactgcgggg gccaagagcg caagcccgaa cctcgtctcg gcaagcgcgt tgccgccgat
     8281 ccagaccctc gacggctatg gccagctggg ctcacctccg gcgcgggcag ctcccatcgc
     8341 gtccgatcaa gtcgacaccg cacag
//
LOCUS       DATNAE010000154         3664 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2253524, whole genome shotgun sequence.
ACCESSION   DATNAE010000154 DATNAE010000000
VERSION     DATNAE010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2253524"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            139..1032
                     /locus_tag="VN518_03405"
     CDS             139..1032
                     /locus_tag="VN518_03405"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HXE03651.1"
                     /translation="MKSWSALLLVAALAAFAPRSALADDAVIARVNGVEIKQSDLDYA
                     ATEIGSRLGNYSPQDRKKVLLQYVIENELMAGAGQTDNLDKTDTFPGRVKYHERRALR
                     DAFFDVKIHDAVSEADAKKIYDEKIGEVKPEQEIHARHILVATEDEAKEIAERLKKGE
                     DFATLAKEKSKDANAEGGDLGWFGRGQMLKPFEDAAFALDVGQISAPVQTQFGWHIIK
                     VEEKRDQPLPTFDQVKEAIIAQLVQAKAQEVVTGLRDSAKIEVVDPELKKSMDEAASK
                     GEDGGPGDLPDDTSGTPDGNN"
     gene            1067..2308
                     /gene="argJ"
                     /locus_tag="VN518_03410"
     CDS             1067..2308
                     /gene="argJ"
                     /locus_tag="VN518_03410"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HXE03652.1"
                     /translation="MLGKISPFAPKSVPRLPVIEGVRIATAAAGIRYPGRIDLLLALF
                     DPGTTVAGVLTNSKTSSAPVDWCRARLAHGMARALVVNSGNANAFTGKRGKQAVTLTA
                     EAAARAADCLEADVYVASTGVIGEPLDAAKIVRQLGVLVREAKADAYADAARAIMTTD
                     TFPKLATRKAEIDGIPVTINGIAKGAGMIAPDMATLLVFLFTDAAIEPRALRASLDPA
                     VDNTFNAVTIDGDTSTSDTLLIFATGRATSRGAPRIVDWDDLRLAGFRLALAEVMHDL
                     ALQVVKDGEGLTKFVSVKVTGAESKRAARVIAKSIANSPLVKTAIAGEDPNWGRIVAA
                     VGKAGEAADRDRLAIWFGDILVAKDGEVAASYAEKDGAAYMQGPEIAITVDVGVGKEA
                     ATIWTCDLTKDYIAINADYRS"
     gene            2352..2768
                     /gene="mutT"
                     /locus_tag="VN518_03415"
     CDS             2352..2768
                     /gene="mutT"
                     /locus_tag="VN518_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HXE03653.1"
                     /translation="MREGSKRPIVIVAACALLDEKGAVLIAKRPPGRPLAGLWEFPGG
                     KIEPGEEPEEALIRELHEELGIEIEKRDLTPLTFASYAYPDFHLLMPVYLCRRWRGEV
                     AAHEGQELLWVKPDTLHLYDMPPADEPLKIAFRDLL"
     gene            2783..2989
                     /locus_tag="VN518_03420"
     CDS             2783..2989
                     /locus_tag="VN518_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015452429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HXE03654.1"
                     /translation="MGHTPSTEPTRLAMRLSAAARRFAADCSGATAIEYGILTLIAIA
                     IMAVVSQIGGSVGGMFDMLKGAFN"
     gene            complement(2986..>3664)
                     /locus_tag="VN518_03425"
     CDS             complement(2986..>3664)
                     /locus_tag="VN518_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007559820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXE03655.1"
                     /translation="EFAALCPSSSLVCDEDLLPFKAQSFNLIVSGLALHRVNDLPGAL
                     IQIRRALHPDGLFMAALLGARALSELRQALLEAEAEIDGGASPRVAPFGDVREYGALL
                     QRAGFALPVADAETLTVLYASPRELMREVRALGGGNVLLARSRAPLSRRTLERAEAIY
                     RERHASPDGRVSASFEIVYLSGWGPDASQQQPLKPGSAAQRLADALGTTERPAGDKAA
                     FPAKPKS"
BASE COUNT          667 a         1225 c         1201 g          571 t
ORIGIN      
        1 cgtttcggcc gggagccgag gaaccccttg gcaaccctat tccccgggtc taaaacccgc
       61 cccggggaag cgaacccaac ccgctcgccc gctctttcta ggagcaggaa ccggcgagca
      121 gaagaagagg acattctcgt gaaaagctgg tcagccctgc tgctcgtggc agccctcgct
      181 gcattcgcgc cgcgctccgc cttggccgac gatgccgtta tcgctcgcgt caatggcgtc
      241 gaaatcaagc aatcggacct cgattatgcg gccacggaga tcggctcgcg gctcggcaat
      301 tattcgccgc aggatcgcaa gaaggtgctg ctgcaatacg tcatcgagaa cgagctgatg
      361 gccggcgctg gccagaccga caatctcgac aagaccgaca cctttccggg ccgggtgaaa
      421 tatcacgagc gccgcgcgct gcgcgatgcc ttcttcgacg tcaagatcca cgacgcggtc
      481 agcgaggccg acgccaagaa gatctatgac gagaagatcg gcgaggtgaa gccggagcag
      541 gagattcacg cccgccatat tctggtggcg acggaagacg aggccaagga aatcgccgag
      601 cgcctgaaga agggcgagga cttcgccacg ctcgccaagg agaaatcgaa agacgccaac
      661 gccgagggtg gcgatctcgg ctggttcggg cgcggtcaga tgctgaagcc gttcgaggac
      721 gccgccttcg ccctcgatgt cgggcagatt tccgcgccgg tgcagaccca attcggctgg
      781 cacatcatca aggtcgagga gaagcgcgat cagccgctgc ccaccttcga ccaggtcaag
      841 gaagccatca tcgcccagct cgtgcaagcc aaggcgcaag aggtggtcac cggcctgcgc
      901 gattccgcca agatcgaagt ggtcgatccc gagctcaaga agtcgatgga cgaagccgcg
      961 agcaagggtg aggacggcgg tccgggcgat ctgccggacg atacgtcagg gacgcccgac
     1021 ggcaataatt gacgccttct ggatggaccg cgctcgctag aggccgctgt tgggcaagat
     1081 ctcgccgttc gcgccgaagt ctgtgccgcg cctgccggta atcgagggcg tgcgcatcgc
     1141 caccgccgcc gcggggattc gctacccagg ccggattgat ctgctgctgg cgttgttcga
     1201 ccccggcacg acggtcgccg gcgttctgac caactccaag acttcgtcgg cgccggtcga
     1261 ttggtgccgc gcgcgtctcg cgcacggcat ggcccgcgcg ctcgtggtca attccgggaa
     1321 cgccaatgcc ttcaccggca agcgcggcaa gcaagcggtg acgctgaccg ccgaagccgc
     1381 ggcgcgggcc gccgactgcc tcgaggccga cgtctatgtc gcttcgaccg gggtgatcgg
     1441 cgagccgctc gatgcagcca agatcgtcag gcagcttggc gtcctggtgc gcgaggccaa
     1501 ggcggacgcc tacgccgatg cggcgcgcgc catcatgacc accgacacct tccctaagct
     1561 cgccacgcgc aaggcggaga tcgacggcat ccccgtcacc atcaacggca tcgccaaggg
     1621 cgccggcatg atcgcgcccg acatggcgac cttgctcgtc ttcctcttca ccgacgcagc
     1681 gatcgagccg cgggcgctgc gcgcttcgct cgaccccgcc gtggacaaca ccttcaacgc
     1741 cgtcaccatc gacggcgaca cctcgaccag cgacaccttg ctcatcttcg ccaccggccg
     1801 cgccacctcg cgcggggccc cgcgcattgt cgattgggac gacctcaggc tggcgggttt
     1861 tcgcctggcg cttgccgagg tgatgcacga tctcgccctt caggtcgtca aggacggcga
     1921 agggttgacc aaattcgtca gcgtgaaagt gaccggggcc gagtcgaagc gggccgcgcg
     1981 cgtcatcgcc aaatccatcg ccaactcgcc cctggtcaag accgccatcg ccggcgagga
     2041 cccgaattgg gggcgcatcg tcgccgccgt cggcaaggct ggcgaagcgg ccgaccgcga
     2101 ccggctggcg atctggttcg gcgatatcct tgtcgccaag gatggcgaag tcgctgcgag
     2161 ctatgccgag aaggatggcg cggcctacat gcaggggccg gagatcgcca ttaccgtcga
     2221 tgtcggcgtg ggtaaggaag cggcaaccat atggacctgc gacctcacca aagactatat
     2281 cgccatcaat gccgactacc ggtcctgagt cgggtattta gacaaaactg cattaacctc
     2341 cggatgccag tatgcgcgag ggctcgaagc gcccgatcgt tatcgtcgcg gcctgcgcac
     2401 tgctcgatga gaagggcgcc gtgctgatcg ccaaacgccc gccagggcgg ccgctcgccg
     2461 gcctgtggga gtttcccgga ggcaagatcg agccgggaga agagccggag gaagccttga
     2521 tccgcgagct gcacgaggag cttggcatcg agatcgagaa gcgggacctg acgccgctca
     2581 ccttcgcgag ctacgcttat cccgattttc acctgctaat gccggtctat ctctgccgcc
     2641 gctggcgggg agaggtggcc gcgcatgaag gccaggagct tctctgggtg aagcccgaca
     2701 cgctgcacct ctacgacatg ccgccggccg acgagccgct caagatcgcg tttcgcgatc
     2761 tattgtgagt ttggggagga ccatgggcca cacaccttcg acggagccga cccggctggc
     2821 gatgcgcctt tcggccgccg cccggcgctt cgctgccgac tgctctggcg ccacggccat
     2881 cgaatacggg atcttgaccc tgatcgccat cgccatcatg gcggtggtca gccagatcgg
     2941 cggctcggtc ggcggcatgt tcgacatgct gaagggcgcc ttcaactaag actttggctt
     3001 ggcgggaaat gccgccttgt cgccggcggg ccgctcggtc gtgccgagcg catcggccag
     3061 acgctgcgcc gcgctccccg gcttgagcgg ctgctgctgg ctcgcatcgg gtccccaacc
     3121 gctgagatag acgatctcga aggaggcgct cacgcgcccg tcggggctcg cgtggcgctc
     3181 gcgatagatc gcttcggccc gctcgagcgt ccttcgcgag agcggcgccc ggcttcgcgc
     3241 cagcagcacg ttgccgccgc ccagcgcgcg cacctcgcgc atcaattcgc gcgggctggc
     3301 atagagcacc gtcagggtct cggcatcggc gaccggcagc gcgaagcccg cgcgttgcag
     3361 cagcgccccg tactcgcgca catcgccgaa cggcgcgacc cgaggacttg ccccgccgtc
     3421 gatctcggcc tcggcctcga gcagcgcctg gcgcagctcg gacaaagcgc gggcgccgag
     3481 cagcgccgcc atgaacaagc cgtcgggatg cagcgcgcgc ctgatctgga tgagcgcgcc
     3541 gggaagatcg ttgacccggt gcagcgccag accggagacg atcagattga agctctgcgc
     3601 cttgaacggc aacaggtcct cgtcgcagac gagcgacgag gaggggcaga gcgcggcaaa
     3661 ttcg
//
LOCUS       DATNAE010000155         3118 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_449714, whole genome shotgun sequence.
ACCESSION   DATNAE010000155 DATNAE010000000
VERSION     DATNAE010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449714"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            260..1246
                     /locus_tag="VN518_03430"
     CDS             260..1246
                     /locus_tag="VN518_03430"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HXE03656.1"
                     /translation="MSPASLKDIEQRCSWRRYEPGEPIVEYLDRSDDVFFLVDGEARV
                     IIYSLTGKVVSFHDLSPGDMFGEYPAIDGRPRSASVEARTSCLVALMPAAAFLDLVQS
                     EPKVSQALLKQLVATIRRLSTRVYEFSAHIMAVTARRIYAEMQGIRAKDSYPNEEVKA
                     YLVEKTFPKSAEEMTLKLSNVSAAFYGLMLAHIGDRFGWREADAISKAVFRKLGQLKT
                     REALELGVDVPKDSRAPALVFITAVHSASPEYNFEVFEYLPDETVIRVFGVSRYDRIA
                     KKLDIERYLSWPELLPFLEGVAEEMGIKCKVEGELKELGEEGRYDCVYRITL"
     gene            complement(1276..1995)
                     /locus_tag="VN518_03435"
     CDS             complement(1276..1995)
                     /locus_tag="VN518_03435"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HXE03657.1"
                     /translation="MAKTANRQPQQLQLSLEGIAILAGLSPEARERLQKRCTWRRCRP
                     GDPIVSYLDSSDDVFFIVEGEVRVTIYSVAGRAVSFRSLGPGEVFGEYPAIDGGPRSA
                     SVEAARSSLIASIPAAAFQELLQTEPKVAQALLPQLVTKIRALTTRVYEFSTLAVNNR
                     IQAELLRLASLAPRQGKAAQIVPSPTHVEIASRVSTHREAVTRELNRLSRIGIIERKR
                     GALLVKDVDRLAEMVHEATGE"
     gene            2204..2674
                     /locus_tag="VN518_03440"
     CDS             2204..2674
                     /locus_tag="VN518_03440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HXE03658.1"
                     /translation="MRLTKQTSYALRILIHCALSGGKHVKAADIAAEHGITEFNVAKI
                     VPILVRGGFITTSRGRTGGLKLARAPQEIGVGDVIRATEATHVEAECVGGTTLPCGIK
                     RAAPINRMLGEAVEAFIEVLDKHTLADLIVARPREAVAGAGALRRARVASTASH"
     gene            complement(2702..>3118)
                     /locus_tag="VN518_03445"
     CDS             complement(2702..>3118)
                     /locus_tag="VN518_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03659.1"
                     /translation="YNTLGHNDCPAKIWAGITEDAMKQRFGALMVLMNGPRFFIMDEI
                     TASGASASGKTIDVDGMELTARASIDLAYVMQAYAQIVDKTLTYDDLPALGAKLKLPS
                     GWRYSTTVPQQDIVAGYEGKATVVQDDLDNTYQKLD"
BASE COUNT          562 a          967 c         1007 g          582 t
ORIGIN      
        1 acgcttttct ctcggagcat ttcgacaaga tcggcaagga gtcgtcgacg tcgatcgagc
       61 agcgcttcct ctgaccggct tcatcgaatt gttacgccct tggggcgccg cgcgccgacc
      121 ccggcatagc gctttcgcat acaacgtgag cggagtattg ttgcccggtt cgagtggggg
      181 ttagatggcg ccggcgatgg cagagtccca aaccgcacaa tcccagcgct ctttggcggc
      241 aatcccgtta ttggcgggtt tgtccccggc gagtctcaag gacatcgagc aacgctgcag
      301 ctggcggcgc tatgagcctg gcgagccgat cgtcgaatat ctcgaccgct ccgacgacgt
      361 gttcttcctc gtcgacggcg aagctcgcgt catcatctat tcgctgaccg gcaaggtggt
      421 gagctttcac gatctttccc caggcgacat gttcggcgaa tatccggcca tcgatggccg
      481 tccgcggtcg gcaagcgtcg aggcgcggac gagctgcctc gtcgccttga tgccggcggc
      541 tgcgtttctc gatctcgtgc aaagcgagcc caaggtctcg caggcgctgc tcaagcagct
      601 cgtggcgaca atccgcaggc tgtcgacgcg cgtctacgag ttcagcgcgc acatcatggc
      661 cgtcaccgcc cgcaggatct atgccgagat gcagggcatc cgcgccaagg acagctatcc
      721 caatgaagag gtgaaggcgt atctcgtgga aaagaccttc cccaaatcgg ccgaggagat
      781 gacgctcaag ctctccaacg tctcagccgc gttttacggg ttgatgctcg cccatatcgg
      841 cgatcgattt ggctggcgcg aggccgacgc catttccaag gccgtgttca ggaagctcgg
      901 ccaactgaag acgagagaag ctctcgagct cggtgtcgac gtgccgaagg attctagggc
      961 gcccgcgctc gtgttcatca ccgcggtgca ctcggcctcg ccggaataca acttcgaagt
     1021 cttcgagtac ctcccggacg agacggtgat cagggtgttc ggcgtctcga gatacgatcg
     1081 catcgccaag aagctcgaca tcgagcgcta tctgagctgg cccgagctcc tgcctttcct
     1141 cgaaggcgtc gccgaggaaa tgggcatcaa atgcaaggtc gagggcgagc tcaaggaact
     1201 cggcgaggaa ggccggtacg actgcgtcta ccggattacg ctgtaaccct gcgcccgcgt
     1261 cccccaagtt tccgcctatt cgccggtcgc ctcgtgaacc atctcggcca ggcggtcaac
     1321 gtccttgacc agaagtgcgc cgcgcttgcg ctcgatgatg ccgatgcgcg acaggcgatt
     1381 gagctctctg gtcacggcct cgcggtgcgt gctcacgcgg cttgcgatct ccacatgggt
     1441 tggcgaaggc acgatctgcg cagccttgcc ttggcgcggg gcgaggctcg cgagccgcaa
     1501 cagctcggct tggatgcgat tgttgacggc aagggtgctg aattcataga cgcgcgtggt
     1561 gagcgcgcgg atcttggtca cgagttgcgg cagcagcgcc tgcgccacct tcggctcggt
     1621 ctgcaacagc tcctggaagg cggcggccgg aattgaagcg atgaggctgc ttctcgccgc
     1681 ttcaacgctt gccgagcggg ggccaccgtc gatcgcagga tattcgccga acacctcgcc
     1741 aggaccgaga ctgcggaagc tcaccgctct gccggccacc gaatagatgg tgacgcgcac
     1801 ttcgccctcg acgatgaaga acacgtcgtc ggaagaatcg agataggaga cgatcggatc
     1861 tcccggccgg cagcggcgcc aggtgcaacg cttctgcagg cgctcgcgag cctcggggct
     1921 gagcccggca aggatggcga tgccttcaag agacagttga agctgctgag gctgcctgtt
     1981 cgctgtcttg gccattgcca ccggcccgtc gcgccatcga tctcggcgcg agccgcacca
     2041 tactccagtc tgggcccgcg aacttggcca aaagcaatgc cggttccttg caaaggcagg
     2101 ggcttgctga gccttccgct ccaggcagct taaggccttg tgcaaccacc gagattgaag
     2161 ctattggcat gctcgcgatc tggtatagtc cggctcctcg accatgcgac tgaccaagca
     2221 aaccagctac gcgctcagga ttctgatcca ttgcgcgctc tccggcggca agcacgtcaa
     2281 ggctgcggat atcgcggccg agcacggcat caccgaattc aacgtggcga agatcgtgcc
     2341 gatcctcgtg cgcggcggct tcatcaccac gagccggggc cgcaccggag gccttaagct
     2401 tgcgcgggcg ccgcaagaga tcggcgtcgg cgacgtgatc cgcgcgaccg aggcgacgca
     2461 tgtcgaggca gaatgcgtgg gcggcacgac gcttccttgc ggcatcaagc gcgcggcgcc
     2521 gatcaaccgc atgctgggcg aggcggtgga agcgttcatc gaggttctgg acaagcacac
     2581 gctggccgat ctgatcgtcg ccaggccgcg cgaggcggtt gcgggcgccg gagccttgcg
     2641 gcgcgcgcgc gtggcgagca ctgccagcca ttaggcgtga gcggccgagg cgtaaccgag
     2701 gtcaatccag cttctgatag gtgttgtcga gatcgtcctg caccacggtc gcttttccct
     2761 cgtagccagc gacgatgtcc tgttgcggca ccgtggttga atagcgccag ccagaaggga
     2821 gtttcagctt ggcgccgagc gcgggcagat cgtcataggt cagggtcttg tcgacgatct
     2881 gcgcgtaagc ctgcatgacg taggcgagat cgatcgaggc gcgcgcggtg agctccatgc
     2941 cgtcgacgtc gatggtcttg cccgaggcgc tcgcgcccga agcggtgatc tcgtccatga
     3001 tgaaaaagcg cgggccgttc atcagcacca tgagcgcgcc gaagcgttgc ttcatggcgt
     3061 cctcggtgat gccggcccag atcttcgccg ggcagtcgtt atggccgagc gtgttgta
//
LOCUS       DATNAE010000156         2016 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_450392, whole genome shotgun sequence.
ACCESSION   DATNAE010000156 DATNAE010000000
VERSION     DATNAE010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450392"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1456)
                     /locus_tag="VN518_03450"
     CDS             complement(<1..1456)
                     /locus_tag="VN518_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
                     /protein_id="HXE03660.1"
                     /translation="MKATAKKVQEKATATKAKAAQAKDEAPEKEAEGAPDTPLPLLDL
                     TDAGVKKMIKAAKKRGYVTHEQINAVMPSEEVTSDQIEDVFAMLNEMGINVVETEEAN
                     DEEGDEKAGEEAADDDDDDSGNELVESKAKSLAVTEKKEPSERTDDPVRMYLREMGSV
                     ELLSREGEIAIAKRIEAGREAMIAGLCESPLTFQAIIIWRDELNDGKVFLRDIIDLEA
                     TYAGPDAKNMQPPQIGPDGKPIAPVPGQPVPGAPHLQVVQTAAPPAAPPAATPFKAPP
                     GAGDEEAEPKDPGESAAESDYDDDDMENSLSLAAIEAELKPKVVDTFDTIADSFKRLR
                     RLQEQDIQFQLKSQSLSPAQERKYKKLKEEIITEVKSLRLNQARIDSLVEQLYDINKR
                     LVSNEGRLMRLAESYGVAREDFLKQYMGGELDPRWLNRVSKLSAKGWKGFVSHEKDRI
                     KEIRSVVHTLAAQTGVEIGEFRKIVHGVQKGEREA"
     gene            complement(1657..>2016)
                     /locus_tag="VN518_03455"
     CDS             complement(1657..>2016)
                     /locus_tag="VN518_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HXE03661.1"
                     /translation="AHAETADAATVRAELSGAGLAEAMARIEAAITTPSVWGAGPDAA
                     PDDVLVTWQQLVSLHRQWHSLLKELKDAEWALGQDNSESNYLRLVDVKARLARVEGTE
                     ALIEGFGTSSGRSARSL"
BASE COUNT          289 a          668 c          641 g          418 t
ORIGIN      
        1 gcgcctcgcg ctcacccttc tgcacgccgt gcacgatctt gcggaattcg ccgatctcga
       61 cgccggtctg cgccgcgagc gtatgcacca ccgagcggat ctccttgatc cggtccttct
      121 cgtgcgagac gaagcccttc cagcccttgg cggacagctt ggagacgcgg ttgagccagc
      181 gcgggtcgag ctcaccgccc atgtactgct tgaggaagtc ctcgcgcgcc acgccgtagg
      241 attcggcaag gcgcatcagc cggccttcgt tggagacgag gcgcttgttg atgtcgtaga
      301 gctgctcgac aagcgaatcg atgcgcgcct ggttgagccg cagcgacttc acctcggtga
      361 tgatctcttc cttgagcttc ttgtatttgc gctcctgcgc cggggagagc gactgactct
      421 tgagctggaa ctggatgtcc tgctcctgca ggcggcgcag gcgcttgaaa ctgtcggcaa
      481 tggtgtcgaa ggtgtcgacg accttgggct tgagctcggc ctcgatggcg gcgagtgaca
      541 gcgaattttc catatcgtcg tcgtcgtaat ccgactcggc ggcggactcg cctggatctt
      601 tcggctcagc ttcctcgtcc ccagcgccgg gcggcgcctt gaatggcgtt gcggctggcg
      661 gcgcggcagg cggcgccgcg gtctgaacca cctgcagatg cggggcaccc ggcaccggct
      721 ggccgggaac gggcgcgatc ggcttgccgt ccggcccgat ctgtggcggc tgcatgttct
      781 tggcgtcggg gccggcgtaa gtcgcctcaa ggtcgatgat gtcgcgcagg aagaccttgc
      841 cgtcgttcaa ttcgtcgcgc cagatgatga tggcctggaa ggtgagcggg ctctcgcaca
      901 gcccggcgat catcgcctcg cggccggctt cgatgcgctt ggcgatggcg atctcgccct
      961 cgcgtgacaa caattcgacc gagcccatct cgcgcaagta catgcgcacg ggatcgtcgg
     1021 tgcgctcgga gggctccttc ttttcggtga cggcgagcga cttcgccttc gattcgacga
     1081 gctcgttgcc ggagtcgtca tcgtcgtcgt cggcggcctc ttcgcccgcc ttctcgtcgc
     1141 cctcctcgtc attggcctcc tcggtctcga cgacgttgat gcccatctcg ttgagcatcg
     1201 cgaagacatc ctcaatctgg tcggaggtta cctcctccga cggcatgacg gcattgatct
     1261 gctcatgggt gacgtagccg cgcttcttgg cggctttgat catcttcttg acgccggcgt
     1321 cggtcaggtc gagcagcggc agtggggtat cgggggcgcc ttcagcttcc ttttcgggcg
     1381 cctcgtcctt ggcctgggcg gccttggcct tggtcgcggt cgccttctcc tgcaccttct
     1441 tggccgtcgc cttcatctca agtccctgct ctgttcgcta cgcggacaac cggtccccta
     1501 ccctatgggt agtgactcga ctccatggcc aaaacgggcc gccgccccgg gcgtgacgcc
     1561 cgaatcaggc cgccagtgtc ctcggggccg tttaagcccc gattaacctc aaaaacacgc
     1621 gaaaatcgac cgctatttca ccaagccgac ccgcgttcac aggctgcggg cgctgcggcc
     1681 ggaggaggtg ccaaagcctt cgatcagggc ttcggttccc tcgacccggg caagtcgcgc
     1741 cttgacgtcg accaggcgga gataattgct ttcgctattg tcttggccaa gcgcccattc
     1801 ggcgtccttg agctccttaa gtagggaatg ccactggcga tgcaagctga cgagctgctg
     1861 ccaggtcacc agcacatcat cgggcgcggc atccggcccc gccccccaga ccgagggggt
     1921 ggtgatggca gcctcgatgc gcgccatggc ctcggccagc ccggcccctg acagctcggc
     1981 gcgcacggtc gcggcgtccg ccgtctcggc atgggc
//
LOCUS       DATNAE010000157         2264 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_451191, whole genome shotgun sequence.
ACCESSION   DATNAE010000157 DATNAE010000000
VERSION     DATNAE010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451191"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1162)
                     /locus_tag="VN518_03460"
     CDS             complement(<1..1162)
                     /locus_tag="VN518_03460"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HXE03662.1"
                     /translation="MSEAKTVSGHVNRRAGRLLAMGGALLAVGIVVCAGARAEDTLSV
                     ATWGGAYGQSQEIAYFEPFAKETGTRIATEVYDGTLAKIKEMIGGSESNVDVVDVSAA
                     ALNTLCSDGLLEPIEASSLGAAPSGESAELDFYSGGLSSCGVGSVAWSTAITFDRQAF
                     TKAQPSQIADLIDAKRFPGKRGLPNGPRYTLELALLADGVEPANVYTELATPEGADRA
                     FKALDKIKSDIFWWDNAQEPITWLLEKKVGMAAGYSGRIFRAAVGDRQRLGMVWDGQI
                     YDLDLWAIPKGAKNKDGAKRFIAFASDPARLATQAQLIAYGPMRKSALALVGKHPIID
                     VEMKDYLPTAPDNFKKALRFDDAWWNEHGADLQKRFDEWRGPVSPGQGKPPIE"
     gene            complement(1173..>2264)
                     /locus_tag="VN518_03465"
     CDS             complement(1173..>2264)
                     /locus_tag="VN518_03465"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HXE03663.1"
                     /translation="EGDVQADMDNARRFFRWAGIALPPGRSLQKLSAAGKARWNGSTL
                     TFDDGTFSLDGNKAVGLLAVTPGPRPRIDATLDFERLAIDPYLDGTDNPTHLDAQAGL
                     LEGALIKYFDADLRISAADISAPMIKLGRGGFTISTKDGVLASEVGELDLCGGEASGS
                     VNVDLSREVAKANLVASLDDVPVEGCLDVSFLVPLKGTTSLKAEMSGEGRTYPELIQA
                     LSGGLKVNATNGAIPLDLARLLAAAPTPLDGEGWSRNSVTLFDSLNADCRLGGGHIWC
                     DTFDMQTRRGLIAGSGNVDMTQATLDWSMFVASDAQPLKASQLSAETPPRISISGALA
                     QPMIRRTDRPTLGDGSLPTGATANQISPR"
BASE COUNT          366 a          763 c          727 g          408 t
ORIGIN      
        1 cttcgatcgg cggcttgcct tgacccggcg acactgggcc gcgccattca tcgaagcgct
       61 tttgcaggtc cgcgccatgc tcgttccacc aggcgtcgtc gaatcgcagc gccttcttga
      121 aattgtctgg cgccgtgggg agataatcct tcatctccac atcgatgatc gggtgcttgc
      181 cgaccagcgc gagcgccgat ttgcgcatcg gcccgtaggc gatcagctgc gcctgcgtcg
      241 ccagccgggc ggggtcggag gcgaaggcga tgaagcgctt ggcgccgtcc ttgttcttgg
      301 cgcctttggg gatggcccaa agatcgagat cgtagatctg cccgtcccac accatgccga
      361 gccgctgccg atcgccgacc gcggcacgga agatgcggcc gctatagccc gccgccatcc
      421 ccaccttctt ttcgagcagc caggtgatcg gctcttgagc gttgtcccac caaaagatgt
      481 cgctcttgat cttgtcgaga gccttgaagg cgcggtcggc gccttcgggc gtggcaagct
      541 cggtatagac attcgccggc tcgaccccgt cggcgaggag cgcaagctcg agcgtgtagc
      601 gggggccgtt cggcagaccg cgcttgccgg ggaagcgctt cgcatcgatc aggtcggcga
      661 tctgcgatgg ctgagccttg gtgaaagcct gccggtcgaa ggtgatcgcc gtcgaccagg
      721 cgacgctgcc gaccccgcag gaggacagcc cacccgaata gaagtcgagt tccgcgctct
      781 ctccgctcgg cgccgcgccg aggctcgagg cttcgatcgg ctcgagcagc ccgtcgctgc
      841 agagcgtgtt gagcgccgcc gcggagacgt ccaccacatc gacgttcgat tccgagccgc
      901 cgatcatctc tttgatcttg gctagtgtcc cgtcataaac ctcggtggcg atccgcgtgc
      961 cggtttcctt ggcgaacggc tcgaaatagg cgatctcctg cgattgccca taggcgcccc
     1021 cccaagtggc gacgctgagc gtatcctccg cccgcgcgcc ggcgcagacg acgatgccga
     1081 cggcgagaag cgcgccgccc atcgccaata gtcgacctgc tcggcggttc acgtgtccag
     1141 acaccgtctt cgcctcactc atggctttgc cgtcaccgcg gcgaaatctg attggcggtc
     1201 gcgcccgtcg gcaatgatcc gtcgccgagc gtcggccgat cggtccgccg gatcatcggc
     1261 tgcgccagcg ccccgctgat ggagatccgc ggcggcgtct ccgcgctgag ttgcgaggcc
     1321 ttcagcggtt gcgcgtcgct ggcgacgaac atgctccaat cgagcgttgc ttgcgtcatg
     1381 tcgacattgc cggatccggc gatcaatccg cggcgggttt gcatgtcgaa cgtgtcgcac
     1441 caaatatggc cgccaccgag gcggcaatcg gcgttcagag aatcgaacaa agtgacactg
     1501 ttgcggctcc agccctcgcc gtccaggggg gtcggcgccg ccgcgagcag ccgggcaaga
     1561 tcgagcggga tggcgccatt ggtggcgtta accttgagcc cgccggaaag cgcctgaatc
     1621 aactcggggt aggtgcggcc ctcgcccgac atttccgcct tgaggctcgt ggtccccttg
     1681 agcgggacca ggaacgacac atcgaggcag ccctcgaccg gcacgtcgtc gagactcgcc
     1741 acgagattgg ccttcgccac ctcccgcgac aggtcgacat tgacgctacc ggaagcctcg
     1801 ccgccgcaaa gatcgagctc cccaacctcg ctcgccaaca cgccgtcctt ggtgctgatg
     1861 gtgaagccgc cacgcccgag cttgatcatg ggggcggaaa tgtccgccgc cgagatgcgc
     1921 aaatcggcgt cgaaatattt gatgagcgcc ccctccagca aaccagcctg cgcgtcgagg
     1981 tgcgtcgggt tgtcggtgcc gtcgagatag ggatcgatgg cgagccgctc gaaatcgagc
     2041 gttgcgtcga tgcgcgggcg cggtcccggg gtcaccgcga gcagaccgac cgccttattg
     2101 ccgtcgagcg agaaggtgcc gtcgtcgaag gtcagcgtcg atccgttcca ccgcgccttg
     2161 cctgctgccg agagtttctg caggctcctt cccggcggca aggctatccc ggcccacctg
     2221 aagaagcgcc gcgcgttgtc catgtcggcc tgcacgtcgc cctc
//
LOCUS       DATNAE010000158         6860 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2858907, whole genome shotgun sequence.
ACCESSION   DATNAE010000158 DATNAE010000000
VERSION     DATNAE010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6860
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2858907"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="VN518_03470"
     CDS             complement(<1..651)
                     /locus_tag="VN518_03470"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXE03664.1"
                     /translation="MVTILQVVPRLDAGGSELATIEIAQALCRVGARALVATEGGGMA
                     EEVARAGGEIVTLPVASKSPFTILANISRLTQLIEARGVDLVHARSRAPAWSAFLAAR
                     RTGKPFVTTYHGAYGEVGPFKAAYNSVMAKGDRVIANSRYTASVIEARGPRVKDRIRV
                     IYRGVDRSAFDPAAVAASAVENLRAQWGVPPGAKIVLHAARLTSIKGQRDLIAAARL"
     gene            705..1487
                     /locus_tag="VN518_03475"
     CDS             705..1487
                     /locus_tag="VN518_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXE03665.1"
                     /translation="MDAPEPQLLEVGAEPRRRRIAYRWRAGEGPALLWLSGFLSDMTS
                     TKATVVADWAAEQGLAMMRFDYSGHGLSGGDLLNAGIGDWLEESMAVWELMGGGPRIV
                     IGSSMGGWIALLLARHLAGQGRTDELAGLVLIAPAFDMTEALMWRELPQEAKDEIAAK
                     GVTYVPSAYGDPYPITRHLIEEGRAHLLEGHPFDPGCPVRILQGMRDPDVPWRHAVAL
                     VDLLDGADVELTLIKDGDHRLSASADLQRLTAAIATLVARQQ"
     gene            complement(1816..2034)
                     /locus_tag="VN518_03480"
     CDS             complement(1816..2034)
                     /locus_tag="VN518_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03666.1"
                     /translation="MVKTLERAIAEVANLPEAAQEKIGQELLAHVERLRNLRTDITSG
                     IQSLDAGAGRELDTEELLRRAREQHGRG"
     gene            complement(2061..2954)
                     /locus_tag="VN518_03485"
     CDS             complement(2061..2954)
                     /locus_tag="VN518_03485"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXE03667.1"
                     /translation="MNRLFCFGLGFSAEALGRRLAAKGWSIAGTARDPANIERIRGQG
                     YEVCRFAGEPGNAELLAALAGTTHLLHSIPPGAEGDPVLRHYRAEIARLPTLEWIGYL
                     STVGVYGNHKGAVVDEAAMPRPHNERTKARVVAESGWLAFGEEIGRPVQVFRLAGIYG
                     PGRSALDKLRAGTARRIVKPGQVFNRIHVEDIASVLEASMARPRAGAIYNVADDEPAP
                     PEDVIAYGAELLGIEPPPEVPFEEADLTPMARSFYGDTRRISNTLIKSELGVRLAYPN
                     YREGLKALIDADRPAKRTSPQ"
     gene            complement(2951..4069)
                     /gene="queG"
                     /locus_tag="VN518_03490"
     CDS             complement(2951..4069)
                     /gene="queG"
                     /locus_tag="VN518_03490"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016839975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HXE03668.1"
                     /translation="MALTLKERIAREANALGFAAVRFASADPPPGAGAALEDFLAHGR
                     HGDMAWLADNKERRKSPRAVWPEVKSIIMLGLSYGPERDPLQALGMRSRGAISVYAQG
                     GDYHDVIKSKLKALAARVQDVAPGEIKIFVDTAPVMEKPIAAKAGLGWQGKHTNLVSR
                     DFGSWLFLGSIFTTAEIDPDAPEEDHCGACQRCLDVCSSKAFVAPYQLDARACISYLT
                     IEHKGHIAIEFREAMGNRIFGCDDCLAVCPWNKFAKGAREAKLAARAGSDNPPLSELL
                     PLDDAAFRARFGGTPIKRTGRDRFLRNVLIAAGNSGDESLVPQVEQRLSDASPLVRAM
                     AVWALSRLAPDWAAAVRESEAQGEADSAVAEEWARIGA"
     gene            complement(4099..4791)
                     /locus_tag="VN518_03495"
     CDS             complement(4099..4791)
                     /locus_tag="VN518_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017485713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HXE03669.1"
                     /translation="MATLLHYPLCPFSRSIRLALAECGVEVTLTEERPWEWRPAFLEL
                     NPAGALPVLVTDSDGPISGAYAISEYLNETVSCEGEAARGFKLFPGEPVARAETRRLV
                     DWFHRKFHDEVTSYLVEEKVYRRYGPGGGSPNTEAVRAGHENLRYHLAYLGHLAETRP
                     WLAGEAPSFADLAAAAHLSTLDYLGEVFWEDYEPVKSWYALLKSRPSFRPLLADRVAG
                     FIPSGSYADLDF"
     gene            4904..5785
                     /locus_tag="VN518_03500"
     CDS             4904..5785
                     /locus_tag="VN518_03500"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF001393.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HXE03670.1"
                     /translation="MPIEQIIVLAVVQGITEFLPISSSGHLILIPFLTGWPDQGQFTD
                     VMSHLGTLLAVLIYFWRDVWHLILGTLNLFRGKITDQGRLALYILAGTIPAVLFGYTL
                     KKLNVPDLERNITVVAWNTVFYGVLMLVADMYGKQEKTINDVTLKSALVIGCAQALAL
                     IPGTSRSGVTMTAARFLNFTRPDAARFSFLLGIPATAAAIVLTLGDAVKSGCHITTDE
                     LLVAAFTFVAGILAIAFLMNLLRRISFLPFVLYRMALGGFLLVLVYGYGAHLSPAPEG
                     NCGPGQAASEQSAPGSQ"
     gene            complement(5797..6768)
                     /locus_tag="VN518_03505"
     CDS             complement(5797..6768)
                     /locus_tag="VN518_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009734652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HXE03671.1"
                     /translation="MPSNIDTLITIFGGSGFIGRHVVRALAKTGARMRIAVRRPELAG
                     FLQPLGGVGQITAVQANVRFPDSLLAPAKGADAVINLVGILCPSGKQTFKAVQDEGAR
                     HVAEAARAAGASAFVHMSAIGADAQSPSVYARSKAAGEAAAKEVFPDAVILQPSVVFG
                     PEDEFFNRFAALAQLSPALPLIGGGKTKFQPVFGGDVAKAVMASLTGKAKAGAVYELG
                     GPEILTMKEVMERVLAYTMRKRWLVPLPFFLAKLQGAVLQLLPNPPLTLDQVRLLERD
                     NVVSDAAKRDGRTLEGLGIGEPVAVEAVVPDYLEQYRPKGQFSDYQP"
BASE COUNT         1180 a         2245 c         2210 g         1225 t
ORIGIN      
        1 gagccttgcc gcggcgatca ggtcacgctg ccccttgatg ctggtgagcc gcgccgcatg
       61 cagcacgatc ttggcgccag gcgggacgcc ccattgagct ctgaggttct cgaccgcgct
      121 ggcagcgacg gcggcgggat cgaaggcgct gcggtcgacg ccccgataga tgacgcgaat
      181 acggtccttc acgcgcggcc ccctcgcctc gatcaccgat gcggtgtagc gcgaattggc
      241 gatgacgcgg tcgcccttgg ccatgacgct gttgtaggcc gccttgaacg gaccaacctc
      301 gccataggct ccgtgatagg tggtgacgaa gggtttgccc gtccgccgcg ccgcgagaaa
      361 ggcgctccac gcaggcgccc ggctccgcgc atgcacgagg tcgacgcctc gcgcctcgat
      421 cagctgcgtg aggcggctga tattggcgag gatggtgaac ggagatttgc tcgccaccgg
      481 gagcgtcacg atctcgccgc cggcgcgcgc cacctcctcg gccatgccgc cgccttctgt
      541 ggcgaccagc gcgcgggcgc cgacccggca aagcgcctgc gcgatctcga tggtcgccaa
      601 ttcgcttccc ccggcatcga ggcgcggcac gacctggagt atggtcacca tgaatgaggg
      661 cttcgcttcc agctattgcc gcgaaaggtc atcagtctct taggatggac gcacccgaac
      721 cgcaattgct ggaggtcggc gccgagcctc gccgccggcg gatcgcctat cgctggaggg
      781 caggggaggg gccggcgctc ctttggctct caggctttct ctccgacatg acgagcacca
      841 aggcgaccgt tgtggcggat tgggcggctg agcagggcct cgccatgatg cgcttcgact
      901 attccggcca tgggctctcc ggcggggatt tgcttaacgc cggcatcggc gattggctcg
      961 aagagtcgat ggcggtgtgg gagctgatgg gcgggggccc acgcatcgtc atcggctcgt
     1021 ccatgggcgg ttggatcgca ttgttgctcg cgcggcacct cgcgggccaa ggcagaactg
     1081 atgagctcgc cggcctcgtg ctgatcgcgc ctgctttcga catgaccgag gctctgatgt
     1141 ggcgcgagct gccgcaagag gcgaaggacg agatcgcggc gaagggtgtc acatacgtgc
     1201 cctctgccta tggcgatcct tatcccatca ccagacatct catcgaggag gggcgcgcgc
     1261 atctcctcga aggccatccc ttcgatccgg ggtgcccggt gcgcatcctg caaggcatgc
     1321 gcgatcctga cgtgccgtgg cgccatgcag ttgcgctggt cgatctgctg gatggggccg
     1381 atgtcgagct gacgctgatc aaggacggcg accatcgcct gtccgcgtcg gcggacttgc
     1441 aacgcctcac cgccgccatc gcgacgctcg tcgcccggca gcagtgaatt gggagggagg
     1501 caacctcact cggcgtcctg atcgtccgcg aatatcgaat caatatctct gcgaccatgg
     1561 aggacgcgga caatctcaac tgccttcccg gtcaaacgat agaataccac gtaaggattg
     1621 gccgctaccg atcgtaacat tggcgcgagc tcctcgcgca aacgcccggc aaggggatgg
     1681 tcgccgagga gccggcacgc atgaacgcta tcgcgaatct tagtgtcggc gactgtgaat
     1741 gacgcttcgc gcgcgagata gctccaaata tcgagtaggt cctgttcggc ttccggcgac
     1801 cagataatgc gatgcctagc cgcggccatg ctgctcgcgg gctcgcctca ggagctcctc
     1861 ggtgtcgagc tcgcgcccgg cgcccgcgtc gagcgattgg ataccgctag tgatgtccgt
     1921 ccgcaggttt cgcaggcgct cgacatgcgc cagcaattcc tggccgatct tttcttgtgc
     1981 cgcctccgga agattcgcaa cttccgcgat ggcgcgttcc agcgtcttga ccatatcggc
     2041 actctaccac atcgctggct tcactgggga ctagtgcgtt tcgcgggacg atcagcgtca
     2101 atgagcgcct tcagcccctc ccgatagttc ggataggcga gccgcacccc gagctctgac
     2161 ttgatcagcg tatttgagat gcggcgggtg tcgccataga agctgcgcgc catgggcgtg
     2221 agatcggcct cctcgaacgg cacttccggc ggcggctcga tgcccaacag ctcagcgcca
     2281 taagcgatga cgtcctcagg cggcgccggc tcatcgtcgg cgacgttgta gatggcgccc
     2341 gcccgcggcc gcgccatcga ggcttcgagc acgctcgcga tgtcctcgac atggatgcga
     2401 ttgaatacct ggccaggctt gacgatgcgc cgcgccgtgc cggctctgag cttgtcgagc
     2461 gcgctccggc ctgggccata gatgccggca aggcggaaca cctgaaccgg ccgcccgatc
     2521 tcttcgccga aggcgagcca gccgctctcg gccacgaccc gcgctttggt gcgctcgtta
     2581 tggggacgcg gcatggcggc ctcatcgacc acggcgccct tatggttgcc gtagaccccg
     2641 acggtcgaga ggtagccgat ccattcgagc gtcgggagac gcgcgatctc ggcgcgatag
     2701 tggcggagca cgggatcgcc ctcggctcca ggcggaatcg agtggagaag atgggtggtt
     2761 ccggcgagcg ctgccaacaa ttccgcattg cccggctccc cggcaaagcg gcagacctca
     2821 tagccctggc cgcgaatgcg ctcgatattg gcggggtcgc gggcggtgcc ggcaatgctc
     2881 cagcctttag ccgcaagccg ccgccccagc gcctcggccg agaagccgag tccgaagcag
     2941 aacaggcgat tcacgcgccg atcctcgccc attcttcggc aactgctgaa tcggcttctc
     3001 cttgcgcctc agattcgcgc accgccgccg cccaatcagg cgcaagccgc gacaacgccc
     3061 acaccgccat ggcgcgcacg agcggcgagg cgtcggaaag acgctgctcg acctgcggga
     3121 cgagactctc atcgcctgaa ttgccggcgg cgatcagcac attgcgcaag aaccggtcgc
     3181 gcccggttct tttgatcggc gtgccgccga agcgggcgcg aaaggccgca tcgtcgagcg
     3241 gcaacaactc agagagagga ggattgtcgc tcccggcccg cgcggctagc tttgcctcgc
     3301 gcgcaccctt cgcgaatttg ttccacgggc acacggcgag acagtcgtcg cagccgaaga
     3361 ttcggttgcc catggcctcg cggaactcga tcgcgatatg ccccttgtgc tcgatggtga
     3421 ggtaggagat gcaagcccgc gcatcgagct gatagggcgc gacgaacgcc ttagacgagc
     3481 acacatcgag gcagcgttgg caggcgccgc aatgatcttc ctcgggcgcg tcggggtcga
     3541 tctcggcggt ggtgaaaatc gagccgagga acagccacga gccgaaatcg cgcgagacga
     3601 gattggtgtg tttgccctgc cagccgagac cggccttcgc cgcgatcggc ttttccatca
     3661 ccggtgcggt gtcgacgaag atcttgatct cgccgggtgc cacgtcctgc acgcgcgcgg
     3721 cgagcgcctt gagcttcgac ttgatcacgt cgtgataatc gccgccttgc gcgtagaccg
     3781 agatggcgcc gcgcgagcgc atgccaagcg cttgcagcgg gtcgcgctcg gggccgtagc
     3841 taagcccaag catgatgatg cttttgacct cgggccagac ggctcgggga gatttgcgcc
     3901 gctctttgtt gtcggcgagc catgccatgt cgccgtggcg gccatgcgcg agaaaatctt
     3961 ccaaggcggc gccggcgccc ggcggcgggt cggcgctcgc gaaccgtacc gcggcgaagc
     4021 ccaatgcatt ggcctcgcgt gcgatccgtt ctttcagcgt aagcgccatg acacatctac
     4081 tcgacaatcg cccgcagttc agaaatcgag gtcggcatag ctccctgacg ggatgaagcc
     4141 ggcgacccgg tcggcgagca atggccggaa cgacggtctc gacttgagca gggcgtacca
     4201 gcttttgacc ggctcgtagt cctcccagaa cacctcgccg agatagtcaa gcgtggagag
     4261 atgcgccgcc gctgcaagat cggcgaagct cggcgcctct ccggcaagcc aaggcctcgt
     4321 ctcggcgaga tggccgagat aagcgagatg atagcgcaag ttctcgtggc cggcgcgcac
     4381 cgcctcggta tttggcgagc ccccgcccgg gccgtagcgg cgatagacct tctcctcgac
     4441 cagataggag gtgacctcat cgtggaactt gcggtggaac cagtccacca gccggcgcgt
     4501 ctcggcgcgc gccacgggct cgccggggaa gagcttgaag cctctggcgg cttcgccctc
     4561 gcagctcacg gtctcgttga gatattcgga gatggcgtag gcgccggaga tcgggccgtc
     4621 ggaatcggtg accagcacgg gcagcgcccc cgccgggttc agctcgagaa aggccggacg
     4681 ccactcccaa ggccgctcct cggtgagcgt cacttcgacg ccgcactcgg cgagcgcaag
     4741 gcgaatcgag cgggaaaagg ggcagagtgg ataatgcagg agcgtcgcca tcgacccatt
     4801 tcggcggctt tagctccgcg aaaagaatcg cgcggagttg cgcctcggta tagaacctgc
     4861 ccgcgcgccg aacaagcggc catctcgggg gccgagggaa tcaatgccga tagaacagat
     4921 catcgtgctt gccgtggttc aggggatcac ggaattcctg cccatttcct cctccggcca
     4981 cctcattctc atcccgttcc tgaccggttg gccagaccag ggccaattca ccgacgtcat
     5041 gtcgcatctc ggtacgctcc tcgccgtgct catctatttc tggcgcgatg tctggcacct
     5101 tatcttggga acgctcaacc tcttccgcgg caagatcacc gaccagggcc ggctcgccct
     5161 ctacattctc gccgggacca tcccggccgt cctcttcggc tacacactca agaagctgaa
     5221 cgtcccggac cttgaacgaa acatcacggt cgtggcctgg aacaccgtgt tttacggagt
     5281 gctgatgctg gtcgccgaca tgtacggcaa gcaggagaaa accatcaatg acgtgacgct
     5341 gaaaagcgct cttgtgatcg gatgcgcgca agcgctcgcc cttattcccg ggacgagccg
     5401 ttccggcgtc accatgactg ccgcgcgctt tctgaatttc acccgccccg atgcggcgcg
     5461 cttttcgttc cttctcggca ttccggccac cgctgccgcg atcgtgctca ccctcggcga
     5521 cgcggtgaaa agcggatgtc acatcaccac cgacgagttg ttggtcgcgg ctttcacctt
     5581 cgtcgccgga attttggcaa ttgccttctt gatgaacttg cttcggcgga tcagcttctt
     5641 gcccttcgtg ctttaccgca tggcgctcgg cggcttcctg ctcgtgctcg tctacggcta
     5701 tggcgcgcat ttatcgccgg cgccggaagg aaattgcggc cctggccaag cggcaagcga
     5761 gcaatcggcg ccgggctcgc aatagatcgg cgtgcctcag ggttggtagt cagagaactg
     5821 gcccttgggg cggtactgct cgagataatc tggaactacc gcctcgaccg cgaccggctc
     5881 gccgatgccg aggccctcga gcgtgcggcc gtcacgcttg gcggcgtcgc tcaccacatt
     5941 gtcgcgctcc aagaggcgca cctgatcgag ggtgagcggc gggttcggca gaagctgcag
     6001 gaccgcgcct tgaagcttgg cgaggaagaa ggggagcggc acaagccagc gcttgcgcat
     6061 ggtgtaggcg agcacgcgct ccatcacctc tttcatcgtg aggatctccg gcccgccgag
     6121 ctcgtacacg gcgcctgctt tcgccttgcc ggtgaggctt gccatcaccg ccttggccac
     6181 atcgccgccg aacaccggct gaaacttggt cttgccgccg ccgatcaggg gcagcgccgg
     6241 ggagagctgg gcaagggcgg cgaagcggtt gaagaactcg tcctcaggcc cgaacaccac
     6301 cgacggctgc aggatcacag cgtcggggaa cacctctttt gccgccgcct cgccggcggc
     6361 cttgctgcgg gcgtaaaccg agggcgattg ggcatcggcg ccgatcgccg acatatgcac
     6421 gaacgcgctt gctcccgcgg cgcgcgccgc ctcggcaacg tggcgcgcac cctcatcctg
     6481 cacggccttg aaggtctgct ttccgctcgg gcaaagaatg ccgacgagat tgatcaccgc
     6541 atcggcgccc ttcgcaggcg caaggagcga gtcaggaaag cgcacattgg cctggactgc
     6601 ggtgatctgg cccacgccgc caagcggctg gagaaagcct gcaagctctg gcctgcgcac
     6661 ggcgatccgc atcctcgccc cggtcttggc cagcgcgcgg accacatggc gcccgatgaa
     6721 ccccgacccg ccgaaaatcg tgatgagggt gtcgatattg gagggcatgg attgcgttcc
     6781 tgtaccgttc aaagccgttc gggcgcggtt ttattgccct tttcgcgata cgccaacagc
     6841 ccggcactgt acagggtgcg
//
LOCUS       DATNAE010000159         2188 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3458342, whole genome shotgun sequence.
ACCESSION   DATNAE010000159 DATNAE010000000
VERSION     DATNAE010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3458342"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="VN518_03510"
     CDS             <1..498
                     /locus_tag="VN518_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HXE03672.1"
                     /translation="TRGLIGYQGELLTDTRGTGIMNRLFHEYASHKGPIQGRRAGVLI
                     STDAGEAVAYALWNLEDRGPMMIEPGWKVYRGMIVGEHTRDNDLEVNVLKGKKLTNIR
                     TTSKDEAVRLTPPLRMTLERALAYIQDDELVEVTPKSIRLRKRHLDPNERKKAERQRL
                     AEDVA"
     gene            654..920
                     /locus_tag="VN518_03515"
     CDS             654..920
                     /locus_tag="VN518_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03673.1"
                     /translation="MLKAAGLSLALALIATTGAYAKDDDEAKVPAAEVEKVEATLSTL
                     GCKGYEGLKKEEQGIYEIDDAKCAMGTVDIKLDKDYTVILISRY"
     gene            1073..1579
                     /locus_tag="VN518_03520"
     CDS             1073..1579
                     /locus_tag="VN518_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXE03674.1"
                     /translation="MPVDVAHIRPARLKDAAALAAAYEEAWRSAYQGIIPHISLQRML
                     ARRGLRWWESALRKRTPLLVLDFSGEAAGYATYGRCRLGRAPFQGEIFELYLHPIYQG
                     LGLGEKLFDGARARLAELRLKGLLIWALADNDAACGFYLRLGGKPVAEGAESFGDISL
                     RKVAFAWT"
     gene            1674..>2188
                     /locus_tag="VN518_03525"
     CDS             1674..>2188
                     /locus_tag="VN518_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03675.1"
                     /translation="MAESAITEIGARDRLVMTGLVLLGLLTVIAGVLSGRSTIDYVLG
                     RDAREAALSWAGQINKSLAEQGPQAPQLLDQSVDVLNAQAFASQAVPATLPSSSTAND
                     DFPLIEQIDRLTTGWALSHLDRSQSDFVSQLKGFAVLAPDRTPLAIGGKLTPADLTAL
                     LGQADNKAALA"
BASE COUNT          410 a          703 c          677 g          398 t
ORIGIN      
        1 acccgcgggc tgatcggcta tcagggcgag ctcctcaccg acacgcgcgg caccggcatc
       61 atgaaccgcc tgtttcacga atacgcgtcg cataagggtc cgatccaggg tcgccgagcc
      121 ggcgtgctca tttccaccga cgctggcgag gcggtggcct atgccttgtg gaatctcgag
      181 gatcgcgggc cgatgatgat cgagcccggc tggaaggtgt atcgcggcat gatcgtcggc
      241 gagcataccc gcgacaacga cctcgaagtg aacgtgctca agggcaagaa gctcaccaat
      301 atccgcacca cctccaagga cgaggcggtc aggctcacgc cccccttgcg catgacgctg
      361 gagcgcgcgc tggcctatat ccaggacgac gagctggtcg aggtcacgcc gaaatccatc
      421 cgtttgcgca agcgccatct cgatccgaac gagcgcaaga aggccgaacg ccagcgcctt
      481 gccgaggatg ttgcgtagtg ttcctggcaa atattgccgt cacttagcac agcaattttc
      541 cagtattggc ctgcatcttt tgatccatcc caacattgtc tttgccgtgc gcaggtaatt
      601 tgcgacacgc cggcagtcgc cggcgcgaat tagattcagc cggaggaatt catatgctta
      661 aagctgccgg tctatcgctc gctttggctt taatcgccac gacgggtgcc tacgccaagg
      721 atgacgatga ggccaaggtg cccgccgccg aggtcgagaa ggtcgaggcc actctctcca
      781 cgctcggctg caagggatac gaaggcctga agaaggaaga gcagggcatc tacgagatcg
      841 acgacgccaa gtgcgcgatg ggcacggtcg atattaagct cgacaaggac tacacggtga
      901 tcttgatctc gcgctactaa accgcttcga cgctgcgagc gtcgcacacg cgacaaccaa
      961 gaggcgagcc gatgcgaagt cggctcgcct tttttgtcgc gcgaaaaact ctttcgggaa
     1021 aatgccgacg acccctttgc cgagcgctgc catcctgcgc taggctgcgg tcatgcccgt
     1081 cgacgtcgct catatccggc cagcgcgatt gaaagatgcc gcggcgctgg ccgccgccta
     1141 tgaggaagcc tggcggagcg cctatcaggg catcattccg cacatctcgc tgcaacgcat
     1201 gctcgcgcgg cgcgggcttc gctggtggga gagtgcgttg agaaagcgca cgcctctgct
     1261 cgtgctcgac ttcagcggcg aggccgcggg ttatgccacc tacggccgct gccgcctcgg
     1321 ccgcgcgcca ttccagggcg agatcttcga gctctatttg catccgatct atcaaggcct
     1381 cggccttggc gagaagctgt tcgacggcgc gcgcgcgcgg ctcgccgagc tcaggctgaa
     1441 aggcctcctg atctgggcgc tcgccgataa cgacgctgcc tgcggtttct atctgcggct
     1501 cggcggcaag ccggtcgccg agggcgccga gagcttcggc gacatctcgc tgcgcaaggt
     1561 ggcgttcgct tggacgtaac gccgcattaa gcttgcgttt accgcaaacg ttgggcggcc
     1621 cttaacgccg ctcgcctata agcctccctt cggattttcc tgggggaggc accatggcag
     1681 agagtgcgat aaccgaaatc ggggctcgcg atcggctggt gatgaccggt ctcgtgctgc
     1741 tcggtttgct cactgtcata gccggggtgc tctcggggcg atcgaccatc gactacgtgc
     1801 tcggccggga tgcgcgcgaa gccgcgttga gctgggccgg acagatcaac aagagtctcg
     1861 ccgagcaagg accgcaggcg ccgcaacttc tcgatcagag cgtcgacgtg ctcaatgccc
     1921 aagcattcgc cagccaggcc gtgcccgcga cgcttccctc gagctcgacc gccaatgacg
     1981 acttccctct catcgagcag atcgatcgcc tgacgaccgg ctgggcgctg agccacttgg
     2041 accgatcgca gagcgacttc gtgagccagc tgaagggctt cgccgtgctc gctcccgatc
     2101 ggaccccgct ggccatcggc ggcaagctca cgcctgccga ccttaccgcc ctgctgggcc
     2161 aagcggacaa caaggccgcg ctggcgca
//
LOCUS       DATNAE010000160         6663 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_4366012, whole genome shotgun sequence.
ACCESSION   DATNAE010000160 DATNAE010000000
VERSION     DATNAE010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6663
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4366012"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1499)
                     /locus_tag="VN518_03530"
     CDS             complement(<1..1499)
                     /locus_tag="VN518_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HXE03676.1"
                     /translation="MIAETGHYALILALALAIVQTVLPLWGTRTRDPQLMAVAAPAAQ
                     MQFVLIAIAFSALMTAYVTSDFSLQNVWANSHSAKPLIYKIAGVWGNHEGSMVLWVLI
                     LALFGAAVATFGTNLPRELQANVLAVQGSIAAAFLLFIITTSNPFIRLDPAPLDGQGL
                     NPVLQDPALAFHPPFLYTGYVGFSMAFSFAVAALIDGRIDAAWARWVRPWTLAAWMCL
                     TLGIAMGSWWAYYELGWGGFWFWDPVENASFMPWLAGTALLHSALVMEKRDALKVWTI
                     LLAIITFSLSLMGTFLVRSGVLTSVHAFAVDPARGIFILAIMGLFIGGALTLFAWRAP
                     LLSQGGLFAPISREGALVLNNLLLTTACAAVFVGTLYPLALDAITGDKISVGAPFFNL
                     TFAPLIVPLLIAVPFGPLLAWKRGDLLAAAQRLMAAACVVLAVIIAVAAFQQRGPWLA
                     PLGIGLGVWLIAGALTDILYRVKLFETGRPEAWRRLRNLPRSAYGTALA"
     gene            complement(1503..1976)
                     /gene="ccmE"
                     /locus_tag="VN518_03535"
     CDS             complement(1503..1976)
                     /gene="ccmE"
                     /locus_tag="VN518_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HXE03677.1"
                     /translation="MTRKQRRAIMIGTCLGVLALAVGLVLMALRDSIVFFYTPSEVAE
                     KHLDTGQRFRLGGLVENGSLKRGEGTTISFVVTDKRATLPVTYTGVLPDLFREGQGVV
                     AEGALTSEGVFHADSVLAKHDEKYMPPEVAKKLKEQGVWRGDEQAGTPQGSGKVN"
     gene            complement(1993..3135)
                     /gene="ccmI"
                     /locus_tag="VN518_03540"
     CDS             complement(1993..3135)
                     /gene="ccmI"
                     /locus_tag="VN518_03540"
                     /inference="COORDINATES: protein motif:HMM:TIGR03142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HXE03678.1"
                     /translation="MLLWLIFAVLTAAALYVVLRPLMGGSASEESRAAFNATVYRDQL
                     GEVESDRERGLIGEGDAEAARVEIARRLLATDAEKPGAKPSPTHSPTKYLVAGLAVAL
                     PAAALSLYFVYGSPRLPDQPLIARLQDPVSDKNLEVLVARVEARLREHPEEGEGWEVI
                     APVYMGWGRYADAAEAYRQTIRLLGESPKRLANYGQALVLANNGVVSEDARKALERAV
                     VLDPNLIEPRLVLVIAKEQDGQFAAAAEAWRALLAKAPADAPWRKLVEQRLAEDEAKL
                     AGKPVPAKPEGAPGPSPDDVAAAQKMNPADRQAMIETMVQRLDDRLTQKGDDLAGWLK
                     LVRAYTVLDRKDDALKALERAKTQFSGNAEALQQLDALAAELGLKS"
     gene            complement(3314..3787)
                     /locus_tag="VN518_03545"
     CDS             complement(3314..3787)
                     /locus_tag="VN518_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03679.1"
                     /translation="MGTTFLRAASVATLALVPILVAACGSYQGGGFLLGAFSSPVSRN
                     PAASARILVSTPGNGAVIGGIGKSSIGRQMSTNERGIAADAEFRALEYGRSGAVVAWA
                     YPALHHRGSIVPGRPYKRGDQYCRVYTHTINRGGSPEIVKGVACREDNGTWRGVS"
     gene            complement(3868..5193)
                     /locus_tag="VN518_03550"
     CDS             complement(3868..5193)
                     /locus_tag="VN518_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011442333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXE03680.1"
                     /translation="MTAFLLLSLYRHAVERSFDARLNVYLTSLIASTTAEGASTPKAP
                     ANLGEPVFTIPFSGWYWQIKPLEGAAERPLFVSDSLLDQQLKLPSQEGVAADQHLARR
                     AYARGPQDQRLRVVEREIRPAGAQSAPYSYAVAGDAAEIDKDLAEFRMMLIAALGVLG
                     LGLVVATLFQVRFGLSPLRAIRHDLAAIRSGEAERLEGELPTEIRPLQQELNALIQSN
                     REIVDRARTHVGNLAHALKTPLSVITNEARERDDPLASKVVEQAELMRTQITHHLDRA
                     RVAARAGAIGDTTDVDGVLKGLKRALDRIYESRALELTVESSPGLKFQGEKQDFEEMV
                     GNLLDNACKWAKSHVWVSAARCDGAGNFEVLVDDDGPGLTGPDRAKAVKRGQRLDETK
                     PGSGLGLSIVADLAHLYKGRFELEPSPQGGLRARLNLPAALTPSKITVC"
     gene            complement(5252..5923)
                     /locus_tag="VN518_03555"
     CDS             complement(5252..5923)
                     /locus_tag="VN518_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXE03681.1"
                     /translation="MRLLVVEDDPDLNRQLVSALSDAGYAVDTAFDGEEGHFLGDTEP
                     YDAVVLDLGLPKKDGLSVLEQWRRDDRKMPVLILTARERWSDKVTGMDAGADDYVAKP
                     FHMEEVLARLRALLRRSAGHARNELECGRLRLDTKSARVTVDGQAVKLTSHEYRVLAY
                     LMLHRARVVSRTELVEHLYEQDFDRDSNTVEVFIGRLRKKLPVDIIKTVRGLGYRLAD
                     ESDEA"
     gene            complement(5965..6264)
                     /locus_tag="VN518_03560"
     CDS             complement(5965..6264)
                     /locus_tag="VN518_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03682.1"
                     /translation="MRRVIKEAAVAAVVGLLLTAAGQAAEQNCLPWASAAPVIAKNSL
                     LPANIIYQMVQSRTGGQIIHASLCDQDGRFFYKLVVLGAKGDVTNVTVDALTGQP"
     gene            complement(6349..6594)
                     /locus_tag="VN518_03565"
     CDS             complement(6349..6594)
                     /locus_tag="VN518_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03683.1"
                     /translation="MLNLAWLLTGGFAKGYRTQILGLITAISTIALWAVGHTTLTDLI
                     AKLPVIFGGLGLAALGAKVNDASPPAPQTANKSSKAK"
BASE COUNT         1238 a         2111 c         2186 g         1128 t
ORIGIN      
        1 tgggccagcg ccgtgccata ggccgagcgc ggcaaattgc gcaatctccg ccatgcctcc
       61 ggccggccgg tctcgaacag cttcactcga tagagaatgt cggtcagcgc gccggcgatc
      121 agccacacgc cgagcccgat accgagaggc gcaagccatg ggcccctctg ctggaaggcg
      181 gcgacggcga tgatgaccgc gagcacgacg cacgcggccg ccatgaggcg ctgcgccgcg
      241 gccagcaaat ctccccgctt ccacgcgagc agcggcccga atggaacggc gatcaggagc
      301 gggacgatga gcggagcgaa cgtgagattg aagaagggcg cgccgaccga gatcttgtca
      361 ccggtgatcg catcgagcgc gagcggatag agcgtgccga cgaacacggc ggcgcaagcg
      421 gtggtgagca ggagattgtt gaggacgaga gcgccctcgc ggctgatcgg ggcgaacagc
      481 cccccttgcg agaggagcgg cgcgcgccag gcgaacagcg tgagcgcgcc gccgatgaag
      541 aggcccatga tggcgaggat gaagatgccg cgcgcgggat cgacggcgaa ggcgtgcacg
      601 ctcgtcagca cgcctgagcg gacgaggaag gtccccatca gcgacagcga gaaggtgatg
      661 atggcgagca ggatggtcca caccttcagc gcatcgcgtt tctccatcac cagagccgaa
      721 tggaggagcg cggtgccggc gagccagggc atgaaagagg cgttctcgac cgggtcccag
      781 aaccagaagc cgccccagcc aagctcgtaa taggcccacc acgaccccat ggcgatgccg
      841 agtgtgaggc acatccaggc ggcaagcgtc catggcctca cccagcgcgc ccaggcggcg
      901 tcgatgcgcc cgtcgatcaa ggcggcgacg gcgaacgaga aagccatcga gaagccgaca
      961 tagcccgtgt agaggaacgg cgggtggaag gcgagcgccg ggtcctgcaa cacggggttg
     1021 aggccttgcc cgtcgagcgg cgcaggatcg agcctgatga agggatttga cgtggtgatg
     1081 atgaacaaga gaaacgccgc ggcgatcgac ccctgtacgg cgagcacatt cgcttgcagc
     1141 tcgcgcggca ggttcgtgcc aaaggtcgcg accgcggcgc cgaacaaagc gaggatcagc
     1201 acccagagaa ccatcgagcc ttcgtgattc ccccacacgc ccgcgatctt atagatgagc
     1261 ggcttcgccg agtgggagtt ggcccagacg ttctgcagcg agaagtcgga ggtgacatag
     1321 gcggtcatca gagccgaaaa tgcgatcgcg atcagcacga actgcatctg tgccgcgggt
     1381 gcggcgacgg ccatgagttg cgggtcgcgg gtcctcgtcc cccacagcgg aagcactgtc
     1441 tgcacgatcg ccagcgccag cgcgaggatc agtgcgtaat ggccggtctc tgcgatcatg
     1501 gcctagttga ccttccctga gccctgcggt gtgccggctt gctcatcgcc gcgccagacg
     1561 ccctgctctt tcagcttctt ggcgacctca ggaggcatgt atttctcgtc gtgcttggcg
     1621 agcacgctgt cggcgtggaa cacgccttcg gatgtcagtg cgccttcggc taccaccccc
     1681 tggccctcgc ggaaaaggtc gggcagcacg cctgtatagg tcaccggcag cgtggcgcgc
     1741 ttgtcggtaa cgacgaagct gatcgtggtg ccctcgccgc gcttgagact cccgttctcg
     1801 accagtccgc caagcctgaa ccgctgaccg gtatccaagt gcttctccgc cacctcgctc
     1861 ggcgtataga agaacacgat cgagtcgcgc agcgccatga gcaccagacc cacggcgagc
     1921 gcgagcacgc caaggcaggt gccgatcata atggctctgc gctgcttgcg ggtcattcca
     1981 cagaactcca ggtcacgact tcaagccaag ctccgccgcc aaagcatcaa gctgctgcag
     2041 cgcttcggcg ttcccgctga attgcgtctt cgcccgctcg agcgccttca aggcatcatc
     2101 cttccggtcg agcacggtat aggctctaac cagcttgagc caaccggcaa gatcgtcgcc
     2161 cttttgcgtc agccggtcat caagcctttg taccatggtc tcgatcatgg cttggcggtc
     2221 ggcgggattc attttctgcg ccgccgccac atcgtcgggg ctcggtccag gcgctccttc
     2281 cggcttcgct ggaaccggct tgccggcgag cttggcctca tcctcggcga ggcgctgctc
     2341 caccaatttc cgccaaggcg catcggccgg cgccttcgcc agcagcgctc gccaagcctc
     2401 tgccgccgcg gcgaactgac cgtcctgttc cttggcgatg accagcacga gacgtggctc
     2461 gatgagattg gggtcgagca cgaccgcccg ctcgagcgcc ttgcgcgcat cttcggagac
     2521 cacgccattg ttggcgagca cgagcgcttg gccgtaattt gccaggcgct tcggcgactc
     2581 gccgaggagc cggatggtct gacgataggc ttcggccgca tcggcgtagc gcccccagcc
     2641 catataaacg ggagcgatca cctcccaccc ctccccctcc tcgggatgct cgcggagccg
     2701 cgcctcaacg cgcgcgacca gaacctcgag attcttgtcg cttacgggat cctgcaaacg
     2761 ggcgatgagg ggttgatcgg ggaggcgcgg agagccgtat acgaagtaga gactgagcgc
     2821 agcggccggc agagctaccg ctaaccccgc caccaaatat ttggtcggag agtgagttgg
     2881 ggagggtttg gcgcccggct tttccgcatc ggtggcgagc agccggcgag cgatttctac
     2941 gcgggcggcc tcggcatcac cctccccgat caggccgcgc tcgcggtcgc tttcaacctc
     3001 gcccagctga tcgcgataga cggtggcatt gaaggccgcg cgtgactcct cgcttgcgct
     3061 tccgcccatg agcgggcgca acacgacata gagcgcggca gccgtcaaaa cggcaaagat
     3121 cagccagagc aacacctagg gcgggtcctt cttgtcaggg aggctaagcc atagggcaat
     3181 ggcgttaagg ggggaagctt gaggagggag ggataagatg aaatcggcgc aatggaggga
     3241 gtcttcaggg gagccttggc ctggcgccga aggccgggag agccgggaga gaaggcgggc
     3301 gatttcagag gcgtcagctg acgccgcgcc aggttccgtt gtcctcgcgg caggcgacgc
     3361 ccttcacgat ctcaggcgag ccgccgcgat tgatagtgtg ggtatagacg cggcagtact
     3421 ggtcgcccct cttataagga cgccccggta cgatcgaccc gcgatggtgt agcgccggat
     3481 aggcccaggc gaccacggcg ccggaccggc catattcgag ggctcgaaac tcggcgtcag
     3541 cggcaattcc gcgctcgttc gtgctcatct gccggccgat tgagcttttg ccgatgccgc
     3601 cgatcacggc gccatttccc ggcgtggaga caaggatgcg ggcgctcgcg gccggattac
     3661 ggctgaccgg gctcgagaat gctccgagga ggaaaccgcc gccttggtac gacccgcaag
     3721 ccgcgaccag aatcggcacc aaagcgagcg tcgccacgga cgccgcgcgc aaaaacgttg
     3781 ttcccatcga cttttgtcct gaacggacaa ccccagcctt gcgccgacaa agacgcatgg
     3841 tttgagtcga attgtcacag aagacgctta acaaacggtg atttttgagg gggttagggc
     3901 cgcaggcagg ttgaggcggg cgcgcaaacc gccttgcggt gacggctcaa gctcgaagcg
     3961 ccctttatag agatgagcga ggtcggcgac gatggagagc ccaagtcccg accccggctt
     4021 cgtctcatcg agacgctgcc ctcgcttgac cgccttggcc cggtcaggcc cggtaagccc
     4081 cgggccatcg tcatccacca ggacctcaaa attgccggca ccgtcgcatc gggcggcgct
     4141 gacccagacg tgggacttgg cccatttgca ggcattgtcc aggaggttcc ccaccatctc
     4201 ctcgaagtct tgtttctcgc cctggaactt gagcccggga ctggactcca ccgtgagctc
     4261 cagcgcccgg ctttcataga tgcggtccag cgcccgcttc aggcctttga gcacgccgtc
     4321 gacgtcggtg gtgtcgccga tggcgcccgc gcgggcggcg acgcgcgccc ggtcgagatg
     4381 gtgggtgatc tgggtgcgca tcagctcggc ctgttcgacc actttgctcg cgagcggatc
     4441 gtccctttca cgcgcttcgt tggtgatgac gctgagcggt gtcttgagcg catgggcgag
     4501 attgccgaca tgggtgcgcg cccggtcgac gatctcccga ttggattgga tcagggcatt
     4561 gagctcctgc tgcagcggcc ggatctcggt gggaagctcg ccctccagac gttccgcctc
     4621 gccggagcgg atcgccgcga ggtcgtgccg gatcgcacgc aacggcgaga gcccgaaccg
     4681 gacctggaac aacgtcgcca ccacgaggcc aagcccaagc accccgagcg ccgcaatcag
     4741 catcatgcgg aattcggcga gatccttgtc gatttcggcc gcatcgccgg cgaccgcata
     4801 ggaatagggt gccgattgcg cgccggccgg cctgatctcg cgctcgacga cgcgcaagcg
     4861 ttgatcttgc gggccccgcg cgtaggcccg tcgtgccagg tgctggtcgg cggcgacgcc
     4921 ctcttggctc ggcagcttga gctgctggtc gagcaaggag tcggagacga agagcggacg
     4981 ctctgcggca ccttcgagcg gcttgatctg ccaataccag cctgagaagg gaatggtgaa
     5041 gaccggctcg ccgagattgg ccggcgcctt cggggtgctg gcgccctcgg ccgtggtcga
     5101 ggcgatgagg ctggtcagat agacgttgag gcgcgcatcg aagctgcgct cgaccgcgtg
     5161 gcgataaagg gagaggagga gaaatgcggt caccggcaag acgacaagcg tccacgccgc
     5221 cgcggatgcg aagagccgga aggcgagaga attaggcctc atcgctctcg tccgcgaggc
     5281 ggtagccgag cccccggacg gtcttgatga tatccactgg aagcttcttg cgcaggcgtc
     5341 cgatgaagac ctcgaccgtg ttcgaatcgc ggtcgaagtc ctgctcatag agatgctcga
     5401 ccagctcggt ccgggagacc acgcgggcgc gatgcagcat cagataagcg agcacccggt
     5461 attcgtggga ggtgagcttc accgcctgcc cgtcgactgt caccctcgcc gatttggtgt
     5521 cgaggcgaag gcgcccgcat tctagctcgt tccgggcatg ccctgccgag cgtctgagca
     5581 gcgcccgcaa gcgcgccaga acttcctcca tgtggaaggg cttggccaca taatcgtcgg
     5641 cgccggcgtc catgccggta accttgtcgc tccaccgctc acgtgccgtc aggatgagca
     5701 ccggcatctt gcggtcatcg cgccgccact gctcgagcac gctcaaaccg tctttcttgg
     5761 gaaggccgag gtcaagcacc acggcgtcgt aaggctcggt gtcgccgagg aaatgcccct
     5821 cctccccgtc gaaggcggta tcgaccgcat agcccgcatc gctcagcgcc gatacgagct
     5881 gccggttgag gtctggatcg tcttccacga cgagaagccg catcctcata ccctcccgaa
     5941 gcgcttgtcg cgccccagtt cctcctaagg ctggccggtc agagcgtcaa cggtgacgtt
     6001 ggtgacgtcg cccttggccc caagcacgac gagtttatag aaaaaccggc cgtcctgatc
     6061 gcaaaggctc gcatgaatga tctgtccgcc cgtgcgcgat tgcaccatct ggtaaatgat
     6121 attggccggc agcagggaat ttttcgcgat caccggcgcc gccgatgccc agggcaggca
     6181 gttttgttcg gcggcttggc cggctgccgt caacaagagg ccgacgacgg ccgcgacagc
     6241 cgcctctttg atgacccggc gcatggtttt caggttccgc tgctcgtcat tgatctggca
     6301 acgctattgg aacctgcatg aacctcccat gaacgggtct gcaacgggtc atttcgcctt
     6361 gctcgatttg ttggcggttt gcggtgcggg cgggcttgca tcgttaacct tggcgccgag
     6421 cgcggcaagg cccagcccgc cgaagatcac cggcaatttg gcaatgaggt cggtaagcgt
     6481 cgtatggccg acggcccata acgcgatggt cgaaatcgcc gtgatgagcc caaggatctg
     6541 ggtacggtat cccttggcaa aaccgccggt gagaagccag gcaagattca gcattcacgc
     6601 ctccttcttg agaagcacgg ggaaatgaaa tggtcagtgg agcgcgcggt cggcgatgtc
     6661 gga
//
LOCUS       DATNAE010000161         2511 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_755801, whole genome shotgun sequence.
ACCESSION   DATNAE010000161 DATNAE010000000
VERSION     DATNAE010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_755801"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VN518_03570"
     CDS             <1..194
                     /locus_tag="VN518_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03684.1"
                     /translation="CRASTSLSTPKKDVDGRDKPGHDDERESSAADLQPHFFPRTALR
                     FRGNDESFSSQPAQSGAAP"
     gene            complement(191..421)
                     /locus_tag="VN518_03575"
     CDS             complement(191..421)
                     /locus_tag="VN518_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HXE03685.1"
                     /translation="MSEPVILDAKGLKCPLPVLKARKAMRDVPDGGVLRVIATDPGAA
                     KDFEHFCKTTGHHLLSAREEAGTLIFEIRKAG"
     gene            complement(780..>2511)
                     /locus_tag="VN518_03580"
     CDS             complement(780..>2511)
                     /locus_tag="VN518_03580"
                     /inference="COORDINATES: protein motif:HMM:NF012731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HXE03686.1"
                     /translation="RFPNLLANGASGIAVGMATSIPPHNAGEICDALLHLVKHKRCEV
                     AELVELMPGPDFPTGGVLVEAPDAVRQAYATGRGSFRLRARWEVEKLGHGLYQVIVSE
                     IPYQVPKSRLIEKIAQLLEERKLPLLADIRDESTDQVRIVIEPKTRNVDPVVLMESLF
                     RATELETRVPLNMNVLDGQGVPRVMDLKEVLQSFLDHRREVLQRVTRFRLAEIARRTE
                     VLKGQMIVYLNLDEVIRIIREHDDAKERMVKRWRLSEIQVEAILNTRLRALRKLEEME
                     IKRELAALGVEDKELTALLDDAKKQWKAISTELADIRKAFGAGALGKRRTRIGQPPAA
                     VDVPVEALVEREPVTVLCSAKGWIRAVKGHNIPLADMKYKEGDEERFVVPASTTDKLM
                     VFATNGRFYTVSVDKLPGGRGHGEPLRLMIDLDNEHDVAAILPYVAGGKLLLAASDGR
                     GFVVPTDEALSQTRNGKVVLTPGDGAKAIVCTPADGDSVAVVGDNRKLLIFPLADVPE
                     MAKGRGVILQRYHDGELSDAKVFTKADGLTWRQGENRTRTETDLRSWIGERASSGRVV
                     QGFPKNNKFG"
BASE COUNT          399 a          856 c          806 g          450 t
ORIGIN      
        1 tgtgccgggc atccacgtct ttgagcacac ccaagaaaga cgtggatggc cgggacaagc
       61 ccggccatga cgatgaaaga gagtcatcgg cagcagattt gcaaccgcat ttctttcccc
      121 ggacagccct gcgctttcgc gggaatgacg agagtttttc ttcgcaacca gcgcagagcg
      181 gcgccgcgcc ctaacccgcc ttgcggatct cgaagatcag cgtgccggcc tcctcgcgcg
      241 ccgagaggag atggtgcccc gtggtcttgc agaaatgctc gaaatccttg gccgcgccgg
      301 gatcggtggc gatgacgcgg agcacgccgc catcaggcac gtcgcgcatc gccttgcgcg
      361 ccttcaagac cggcaacggg catttgaggc ccttggcgtc gaggatgacc ggctcgctca
      421 ttggagcgct tcagaccatt gaagcccctc tccgcccgct tgcgggagga gagtgaggga
      481 cccatggcgc agcaatggga gggtgaggtg ggcgagcgat gcacgcgtgg ttgcgcagcg
      541 gtgcgagctg cacgacacct caccctccca ttgccaagag gcaatggggc catccctctc
      601 cccacttcgt gcggagaggg ctttcatggc gcatcggcca aatgcgagaa cggtggcgac
      661 atcgcacatg tccgcacatg catccgacga tctcgcgttt gcccccgggt ccccgcttcc
      721 gcatgggaaa gacggtgcaa tgattgcatc cattctggcc atgccttaac gcctgtccct
      781 cagccgaact tgttgttctt ggggaagccc tggacgacgc ggccgctcga ggcgcgctcg
      841 ccgatccaac tgcgcagatc ggtctcggtg cgcgtgcggt tctcgccctg cctccaggtc
      901 aggccgtcgg ccttggtgaa caccttggcg tcgctcagct cgccgtcgtg gtagcgctgc
      961 aggatgacgc cgcgcccctt cgccatctcc ggcacgtctg cgagcgggaa gatgaggagc
     1021 ttgcggttgt cgccgaccac cgccacggaa tcgccatcgg ccggcgtgca gacgatggcc
     1081 ttggcgccgt cgcccggcgt caataccacc ttgccgttcc tggtctggct cagcgcctcg
     1141 tcggtcggca cgacgaagcc ccgcccatcg ctcgccgcga gcagcagctt gccgccggcg
     1201 acataaggca ggatcgccgc cacgtcatgc tcgttgtcga gatcgatcat aagcctgagc
     1261 ggctcgccgt ggccgcgccc gcccggcagc ttgtcgaccg acaccgtgta gaagcggccg
     1321 ttggtggcga acaccatgag cttgtcggtg gtcgaggcgg gcacgacgaa gcgctcctcg
     1381 tcgccctctt tgtatttcat atccgccaag gggatgttgt ggcccttgac ggcgcggatc
     1441 cagcccttgg ccgagcacag gacggtgacc ggctcgcgct cgaccagcgc ctcgaccggc
     1501 acgtcgaccg ccgcgggcgg ctggccgatc cgggtgcggc gcttgccgag cgcgcctgcg
     1561 ccgaacgcct tgcggatgtc ggcgagctcg gtcgagatcg ccttccactg cttcttcgca
     1621 tcatcgagca gcgccgtcag ctccttgtcc tcgacgccca acgcggcgag ctcgcgcttg
     1681 atctccatct cctcgagctt gcgcaaggcg cgcagccgcg tgttgaggat cgcctcgacc
     1741 tggatctcgc tgaggcgcca gcgcttcacc atgcgctcct tggcgtcgtc atgctcgcgg
     1801 atgatgcgga tcacctcgtc gagattgagg tagacgatca tctgtccctt gagcacttcg
     1861 gtgcgccgcg cgatctccgc cagacggaag cgcgtcaccc gctgcagcac ctcgcggcga
     1921 tggtcgagga aggattgcag cacctccttc aggtccatca cccgcggcac gccctggccg
     1981 tcgagcacgt tcatgttgag cggcacgcgc gtctcgagct cggtggcgcg gaacagcgat
     2041 tccatgagca cgaccggatc gacgtttcgc gtcttgggct cgatcacgat gcgcacctgg
     2101 tcggtcgatt catcgcggat atcggcaagc agcggcagct tccgctcttc caatagctgc
     2161 gcgatcttct cgatcaggcg cgacttcggc acctggtacg ggatctcgct gacgatcacc
     2221 tggtagagcc cgtgcccgag cttctcgacc tcccagcgcg cacgcaggcg gaagctgccg
     2281 cgcccggtgg cataggcctg gcgcaccgcg tcgggcgcct cgaccagcac gccgccggtc
     2341 gggaaatcgg gcccgggcat gagctcgacc agctccgcca cctcgcagcg cttgtgcttc
     2401 accagatgca agagcgcgtc gcagatctcg cccgcattgt gcggcgggat gctggtcgcc
     2461 atgccgacgg cgatgccgct ggcaccattg gccagcaggt tggggaagcg c
//
LOCUS       DATNAE010000162         2283 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3765521, whole genome shotgun sequence.
ACCESSION   DATNAE010000162 DATNAE010000000
VERSION     DATNAE010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3765521"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(217..363)
                     /locus_tag="VN518_03585"
     CDS             complement(217..363)
                     /locus_tag="VN518_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HXE03687.1"
                     /translation="MPTLFRLLMVLGVIAGVVTGSLYVLAEYFQPEPKEISKSLRNVK
                     VRTE"
     gene            582..1214
                     /locus_tag="VN518_03590"
     CDS             582..1214
                     /locus_tag="VN518_03590"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HXE03688.1"
                     /translation="MDDLRLAERKKELKPERLAWIREALAARSIVLIGLMGAGKTAVG
                     RRLANRLELPFTDADNEIETAAGQTVSEIFAEHGEPYFRQGESRVIARLLQAGPQVLA
                     TGGGAYMDARTRANIKARGVSIWLKAELPVLLHRVRRRDNRPLLASDDPDKVMRELME
                     KRYPIYAEADITVESRDVAHDLIVGDVVEALARWLDKEADAAAAKTRGKV"
     gene            1218..>2283
                     /gene="aroB"
                     /locus_tag="VN518_03595"
     CDS             1218..>2283
                     /gene="aroB"
                     /locus_tag="VN518_03595"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HXE03689.1"
                     /translation="MRDRLAALAPEAKETTVRVGLGARSYDIVIGERLLDEAGPRIAA
                     ALPGARSAVVSDANVAALYLPRLKASLGGLFLGEVVVAPGEQSKSFPVLAEVCQRLLD
                     LGVERGDMVIALGGGVVGDLAGFAASILRRGVRLVQMPTTLLAQVDSSVGGKTGIDTP
                     QGKNLIGSFHQPSLVLADISVLATLAPREFRAGYAEAMKYGLLGDAPFFTWLEQTWPQ
                     IFSGEGGPRASAIATSVRAKAAIVEADEREESGTRALLNLGHTFGHALEAFAGYSDRL
                     LHGEAIAIGMRLAFSFSAEQGLCPASDVNRVEKHLVHVGLPGRIADIPGSLPTPEELL
                     QLMAQDKKVKGGKLALVLVRG"
BASE COUNT          387 a          741 c          760 g          395 t
ORIGIN      
        1 acttgcggcg gagcttcgcc ttgtcgctgc gaattccaat ctccggcagg cggtgccgac
       61 ggcccgcgcg gaccttcgtc gccttgcggc ggagctttgc cttgcagcac cgggagctgg
      121 cgccgctcga cctgcgccaa ggcttcgctg cagacgaccg attcccatcc gctgaggacg
      181 gcggcaatcg tgatggcgag gccggcgagg agactgctac tcggtccgca ccttgacgtt
      241 acgcaaggac ttggagattt ccttcggctc aggctggaaa tattcggcga gcacatagag
      301 gctcccagtg acgacaccgg cgatcacccc caagaccatc aacaatcgga agagtgtcgg
      361 catcgaggcg ctcgtgtcct ttagtgttct tatcgttctc cgctagacct tgggcgctct
      421 tgtagccgcg aagggggcct tattatgacc ccgaccgcaa ggcgccgtgc ggcccgcgct
      481 atgatggacg attcgaaagg ccgttgtcag cacgagaaaa tcgtggaacc gtgccgccac
      541 ggatcgcgac gggcggcaag ctcggagcgc cgttttgacg aatggatgac cttcgactgg
      601 ctgagcgcaa gaaagaactg aagcccgagc gcctggcatg gatccgcgag gcccttgcag
      661 caaggagcat cgtgctgatc ggcctgatgg gagccggcaa gacggcggtg ggccgccggc
      721 ttgccaatcg gctcgaactc cccttcaccg acgccgacaa cgagatcgaa accgccgcgg
      781 gtcaaaccgt gagcgagatc ttcgccgagc acggcgagcc ttattttcgt cagggtgaat
      841 cgagggttat cgcccgcctg ctgcaggccg ggccgcaggt cctcgccacc ggcggcggcg
      901 cctatatgga cgcccgcacc agggccaata tcaaggcacg cggcgtctcg atctggctca
      961 aggccgagct tccggtgctg ctccatcgcg tgcggcggcg ggacaaccgg ccgcttctcg
     1021 cctccgacga ccccgacaag gtgatgcgcg agctcatgga aaagcgctat cccatctacg
     1081 ccgaggccga tatcaccgtg gagagccggg acgtggccca cgatttgatc gtgggcgacg
     1141 tcgttgaggc gctcgcgcgc tggctcgaca aggaggccga cgcggcggcc gcaaagaccc
     1201 gcggcaaggt gtgaaggatg cgagaccgac tggccgcttt ggcgcccgag gcaaaagaga
     1261 ccaccgtcag ggtggggctt ggcgcgcgaa gctacgacat cgtcatcggc gagcggctgc
     1321 tcgatgaggc tgggccgcgc attgccgcag cacttcccgg cgcgcgctca gccgtggtga
     1381 gcgacgccaa cgtggcggct ctttatcttc cgcggctgaa ggcgagcctt ggcgggctgt
     1441 tccttggcga ggtggtggtg gcgccaggcg agcagagcaa gagcttcccc gtgctcgcgg
     1501 aagtgtgcca gcgtctgctc gatcttggcg ttgagcgcgg cgacatggtg atcgcgctcg
     1561 gcggcggcgt ggtcggcgac cttgccggct ttgccgcaag catcctccga cgcggcgtcc
     1621 gcctcgtgca gatgccgacg acgctgctcg ctcaggtcga ctcctcggtc ggcggcaaga
     1681 ccggcatcga cactccccaa ggcaagaatt tgatcggcag cttccaccag ccgagcctgg
     1741 tgctcgccga tatttccgtg ctggcgacgc tcgccccccg cgagtttcgt gccggctacg
     1801 ccgaggcgat gaaatacggg ctccttggcg acgcgccgtt cttcacttgg ctggagcaga
     1861 cctggccgca aatcttttcc ggcgagggag ggccacgcgc ctctgccatt gccacgagcg
     1921 tccgcgccaa ggccgctatc gttgaggccg acgagcgcga ggagagcggc acccgggcgc
     1981 tgctcaatct cggccatacg ttcggccacg cactcgaagc cttcgccggt tactcggacc
     2041 ggctcctgca tggcgaagcc atcgccatcg gcatgcggct tgccttctct ttctccgccg
     2101 agcagggtct ttgcccggcg agcgacgtaa accgtgtcga gaagcatctc gtgcatgttg
     2161 ggctccctgg ccgcatcgcc gacattcctg gcagcctgcc cacgccggaa gagctgcttc
     2221 agctcatggc gcaggacaag aaggtgaagg gcggcaagct tgcgctcgtg ctcgtgcgcg
     2281 gca
//
LOCUS       DATNAE010000163         3020 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_1357079, whole genome shotgun sequence.
ACCESSION   DATNAE010000163 DATNAE010000000
VERSION     DATNAE010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1357079"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1359
                     /gene="ligA"
                     /locus_tag="VN518_03600"
     CDS             <1..1359
                     /gene="ligA"
                     /locus_tag="VN518_03600"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006935935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HXE03690.1"
                     /translation="QCGVYRAMAEWGFPLNPLAKLTTSVEEMLDTYRDIESGRAGLDY
                     DIDGVVYKLDRLDLQQRLGFVSRSPRYAVAHKFPAEKATTILRDIEIQVGRTGALTPV
                     AKLEPVTVGGVVVQNATLHNEDEIARKDVRIGDTVIVQRAGDVIPQILGVVEGKRPKG
                     AKRFSFPTVCPACGSHAVREVNPVTGKEDAVRRCTGGLICPAQRVERLKHFVSRNAFD
                     IEGLGEKHIKAFYDDGLIQSPQDIFTLAGRDRRSTARLDAREGWGGTSAAKLFAAIEA
                     RRRVKLDRFIYALGIAHVGEITGRLLARVYGSIEAFRDAMLAAARDRAGEAYAELDNI
                     EGIGPTVAEAIADFFAEPHNVEVVDELLREVRPEPLEAIDHASPISGKTVVFTGTLEK
                     MTRPEAKARAERLGAKVAGSVSKKTDYVVAGPGAGSKLKEAERLGLKVLSEAEWLALI
                     GA"
     gene            complement(1363..1572)
                     /locus_tag="VN518_03605"
     CDS             complement(1363..1572)
                     /locus_tag="VN518_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03691.1"
                     /translation="MARTIAQALNQVATKSRCPSCGHEVHSTLGEVKRKPQFACPSCG
                     GDCDVREAAAWVELHARKTWLKRVA"
     gene            complement(1612..1839)
                     /locus_tag="VN518_03610"
     CDS             complement(1612..1839)
                     /locus_tag="VN518_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HXE03692.1"
                     /translation="MAAILTTAAFVPQALHIIRHRETKAISLVMYVAFASGVAFWLLF
                     GVLIHNWPIIVSNAITLVLTLAIVAMKLRYG"
     gene            complement(1875..>3020)
                     /locus_tag="VN518_03615"
     CDS             complement(1875..>3020)
                     /locus_tag="VN518_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase family protein P"
                     /protein_id="HXE03693.1"
                     /translation="AFALVPARGKPSLFIDPAKLGDNVRGHLKETAELLPPEALDARL
                     KGLGAKGARVRLDPETTPVRFAHILEAADAKIAAGDDPCILPKAIKNATEIEGARAAH
                     LRDGAAVVRFLAWLDENSPSGRVDEIGAAMKLEEFRRETGELKDLSFDSISASGPHGA
                     VVHYRPTTASNLKLKARSLYLIDSGGQYADGTTDITRTVAIGSPSAEMRRHYTLVLKG
                     HIAVATARFPKGTRGQDIDPFARRPLWQAGLDFDHGTGHGVGSYLSVHEPPQRISRTG
                     SVEIKPGMILSNEPGLYREGSYGIRLENLVLVTPPAAVKGGTREMLGFETLTLVPFDR
                     RLIDPAILNPDELAWLNAYHAEVRRKIEPLLKGTDRAWLRAATARTS"
BASE COUNT          542 a          980 c          990 g          508 t
ORIGIN      
        1 caatgtggtg tctaccgcgc aatggcggag tggggcttcc cgctcaatcc cctcgccaag
       61 ttaaccacga gcgtcgagga gatgctcgac acctaccgcg acatcgaaag cggccgcgcc
      121 ggcctcgact acgacatcga cggcgtcgtc tataagctcg accggctcga cctgcagcag
      181 cgcttgggct tcgtctcgcg ctcgcctcgt tacgccgtcg cccacaaatt ccccgccgaa
      241 aaggcaacca ccatcctgcg cgatatcgag attcaggtcg gccgcaccgg agcgctgacc
      301 ccggtcgcca agctcgaacc ggtgacagtc ggcggcgtgg tggtgcagaa cgccaccctg
      361 cacaacgagg acgagattgc ccgaaaagac gtgcgcatcg gcgacacggt gatcgtccag
      421 cgcgccggcg acgtcatccc gcagattctc ggggtggttg aaggaaagcg gcctaagggc
      481 gcaaaacgat tcagctttcc taccgtctgc ccggcttgcg gcagccatgc ggtgcgcgag
      541 gtcaatcccg tcaccggcaa ggaggatgcg gtcaggcgct gcaccggggg cctgatctgc
      601 ccggcccagc gcgtggagcg gctcaagcat ttcgtgtcgc gcaacgcttt cgacatcgag
      661 ggcttgggcg aaaagcacat caaggccttc tacgacgatg ggttgatcca atcgccgcag
      721 gacatcttca cccttgccgg gcgcgaccgg cgctccaccg caaggctcga cgcgcgggaa
      781 ggctggggcg ggacctctgc ggcgaagttg ttcgcggcaa tagaggcacg ccgtagggtg
      841 aagctcgacc gcttcatcta tgcgctcggc atcgcgcatg tcggcgaaat caccgggcgg
      901 ctgctcgcgc gcgtctatgg cagcatcgag gccttccgcg acgccatgct tgcggcagcc
      961 cgcgaccgcg caggcgaggc ttacgccgag ctcgacaata tcgaaggcat cggtcccact
     1021 gtggccgagg ccatcgccga tttcttcgcc gagccgcaca atgtcgaggt ggtcgacgag
     1081 ctgttgcgcg aggtcaggcc cgagccgctc gaagccatcg accatgcctc gccgatttcc
     1141 ggcaagaccg tggtgttcac cggcacgttg gaaaagatga ctcgccccga ggccaaggcg
     1201 cgggcggagc gcctcggcgc caaggtcgca ggctcggtct ccaagaagac cgactatgtg
     1261 gtggcggggc caggcgcggg ctcgaagctc aaggaagccg agcgcctagg gctgaaggtg
     1321 ctgagcgagg ccgagtggct tgcgctgatc ggggcgtgag tctcaagcaa cgcgcttgag
     1381 ccaggtcttg cgtgcgtgca actcgaccca ggccgcggcc tcgcgcacgt cgcaatcgcc
     1441 gccgcaagac gggcaggcga attgcggctt ccgcttcacc tcgccgagcg tcgaatggac
     1501 ctcgtgcccg caagaagggc aacgcgattt ggtggccacc tggttgaggg cctgtgcgat
     1561 cgtccttgcc attcagccac aatagactga ttcgctcgcc ccgctgaacc cctacccata
     1621 gcggagcttc atcgccacga tggcgagggt caggaccagc gtaatggcgt tggagacaat
     1681 gatcggccag ttgtgaatga gaaccccgaa cagcaaccag aacgccactc cgctggcaaa
     1741 cgccacgtac atcacgagcg agatggcctt ggtctcgcgg tgcctgatga tgtgcagcgc
     1801 ctgaggcacg aaggccgccg tggtcaagat cgcggccacc gctgagaccc aggtttccat
     1861 gagcgctccg ttgatcagct agtgcgcgcc gtggccgctc gcagccaagc acgatcagtg
     1921 cccttgagca gcggctcgat cttgcgccga acttccgcgt gataggcgtt cagccaggca
     1981 agctcgtcag gattgagtat cgcgggatcg atcagccggc ggtcgaaagg cacgagcgtc
     2041 agcgtctcga agcccagcat ctcgcgcgtc ccgcccttga ccgccgccgg cggcgtcacc
     2101 aacacgagat tttccagcct gatgccgtag ctgccttcac ggtagagccc aggctcgttt
     2161 gacaggatca tgccgggctt gatctcgacg ctgcccgtcc gcgagatgcg ctgcggcggc
     2221 tcatgcaccg agaggtagct gccgacgccg tgtcctgttc cgtggtcgaa atcgagccca
     2281 gcctgccaga gcggccggcg cgcaaaagga tcgatatctt gaccgcgcgt cccctttggg
     2341 aaccgcgcgg tggcgacggc gatatgtccc ttcagcacca gcgtgtagtg ccgccgcatc
     2401 tcggcgctcg gcgagccgat ggcgacggtc cgggtaatgt cggtggtgcc gtcggcatat
     2461 tgtccgcccg agtcgatgag gtagagcgag cgcgctttca gcttgagatt gcttgcggtg
     2521 gtgggccggt aatggaccac ggcgccatgc gggcctgaag cggaaatcga gtcgaagctc
     2581 agatccttga gctctcccgt ctcccgccgg aactcttcga gcttcattgc cgcgccgatc
     2641 tcatcgaccc ggcccgaggg ggaattctcg tcgagccaag cgaggaagcg gacgaccgct
     2701 gctccgtcgc ggagatgcgc ggcgcgggcg ccctcgatct cggtcgcgtt cttgatcgcc
     2761 ttgggcagaa tgcaaggatc atcgccagcg gcgatcttgg catcggcggc ttcgagaata
     2821 tgcgcgaagc gcaccggcgt cgtctcagga tcgaggcgga cgcgggcgcc cttcgctcca
     2881 agccccttca gccgtgcgtc gagcgcctcc ggcggaagca gctctgccgt ctccttcaga
     2941 tggccccgca cattgtcgcc gagcttggcg gggtcgatga agagcgatgg cttgccgcgc
     3001 gcaggcacca aggcgaaggc
//
LOCUS       DATNAE010000164         3102 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2873103, whole genome shotgun sequence.
ACCESSION   DATNAE010000164 DATNAE010000000
VERSION     DATNAE010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2873103"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1858)
                     /locus_tag="VN518_03620"
     CDS             complement(<1..1858)
                     /locus_tag="VN518_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HXE03694.1"
                     /translation="MKQKPDNGFPTRDAIVAFIRKNPGKVGTKEIAREFGLKNADRAE
                     LKRLLRDLADHGTITKRGKKVTEIAQLPATVLADITMRDADGELLATPAEWDDEQGEP
                     PRIRIHIPRHARPGTMAGVGDRALLRVERVNDDDVGYRGRVIKVIDHAKSRVLGVFRS
                     TPDGGGRLVPVDKKQAGRELNIARADAAGAVDGDLVSVDIVRSRGFGLTSGKVKERLG
                     SVASEKAISLIAIHAHDIPIGFSRDAMHEAEEAEPATLKGREDWRELPLVTIDPPDAK
                     DHDDAVHAEPDPDPNNKGGFIIHVAIADVAFYVRPGSALDHDALIRGNSVYFPDRVVP
                     MLPERISNNLCSLVPGEPRGALAVRMVIAPDGRKRSHSFHRVLMRSAAKLAYAQAQAA
                     IDGKADDTTGPLLDPILKPLYAAYALVKRARDERDPLELDIPERKILLKSDGTVDRVI
                     VPDRLDAHKLIEEFMILANVAAAEMLEKKSLPLIYRVHDEPTLEKVHNLQEFLKTLDI
                     PFAKAGALRPSLFNRVLAQVSGQDYEPLVNEVVLRSQAQAEYSSENYGHFGLNLRRYA
                     HFTSPIRRYADLVVHRALIRALGLGDGALPDSETPEHLSEVAAQISLTERRAM"
     gene            complement(1859..>3102)
                     /locus_tag="VN518_03625"
     CDS             complement(1859..>3102)
                     /locus_tag="VN518_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HXE03695.1"
                     /translation="AFLENFFRRYVEYDFTADLEEQLDRISNNEISWQQVLKDFWRDF
                     IGAVDDIKDIRVAEVLDVLDNMLGPHIYPQRADGGDVRQCPTCGTGKLNLKAGKFGAF
                     VGCSNYPECRYTRPLAADTEASADRLLGQDPESGLDVNVKAGRFGPYIQLGEQKDYGE
                     DEKPKRAGIPKGTSPGDIELDRALKLLALPREIGKHPETGLPITAGIGRFGPFVKHDK
                     TYASLEAGDEVFDIGLNRAVTLIAEKVAKGPSRGRFGADPGKQLGDHPSLGAVAVKSG
                     RYGAYVTAGGVNATIPSDKEKDTITLAEAIALIDERASKGTNKGGGKAKAMKTAKQAV
                     KAPKAQNTKKAKAEPNETEADEAESAKAVKKASAKKPAKSKSDSVSKARAPVKADAKT
                     SAAKPSAAKTSAKKSAGKARR"
BASE COUNT          485 a          956 c         1041 g          620 t
ORIGIN      
        1 tcatggcgcg acgctcggtc aacgagatct gcgcggccac ttcgcttagg tgctccggcg
       61 tttcgctgtc gggcagcgcg ccgtcgccaa ggccgagggc gcggatcagt gcgcggtgta
      121 cgacgaggtc ggcataacgc cggatcggcg aggtgaagtg cgcatagcgg cgcaggttga
      181 ggccgaaatg accgtaattc tccgatgaat attcggcctg cgcctgcgag cgcaacacca
      241 cctcgttaac cagcggctcg taatcctggc ccgagacctg cgccagcacg cggttgaaca
      301 gcgagggacg cagcgcgccg gccttggcaa agggaatgtc gagcgtcttc aaaaattcct
      361 ggagattatg caccttctcc aaggtcggct cgtcatggac gcggtagatc agcgggagag
      421 acttcttctc gagcatctcc gctgccgcga cgttggcgag gatcatgaac tcctcgatca
      481 gcttgtgcgc gtcgagccgg tcggggacga tgacgcgatc gacggtgccg tcggatttga
      541 gcaaaatttt gcgttcggga atgtcgagct cgagcggatc gcgctcgtcg cgcgcgcgtt
      601 tcaccagcgc gtaggcggcg taaagcggtt tcaggatcgg atcgagcagc gggccggtgg
      661 tgtcatccgc cttgccatcg atggcggcct gcgcctgcgc ataggccagc ttggcagccg
      721 agcgcatcaa cacgcgatgg aagctgtgcg agcgcttgcg tccatcgggc gcgatgacca
      781 tccgcaccgc gagcgcgccg cggggctcgc ccggcaccag cgagcaaaga ttgttggaga
      841 tgcgctcggg cagcatcggc accacgcggt ccgggaaata gaccgagttg ccgcggatca
      901 gcgcgtcatg gtcgagcgcg gagccgggac ggacatagaa ggcgacgtcg gcgatcgcga
      961 catggatgat gaagccgccc ttgttgttgg ggtcgggatc aggctctgca tgcacggcgt
     1021 cgtcatgatc tttcgcatcg ggcgggtcga tcgtgaccag cggcagctcg cgccaatcct
     1081 cgcggccctt gagcgtcgcg ggctcggctt cctcggcctc gtgcatggca tcgcgcgaaa
     1141 agccaatggg aatatcatgg gcatgaatgg caatcaggct gatcgctttt tcgctggcga
     1201 ccgagccgag ccgctccttc accttgcccg aggtgaggcc gaagccgcgg ctgcggacaa
     1261 tgtcgacgct gacgaggtcg ccatccacgg cgccggcggc gtcggccctg gcgatattga
     1321 gctcacgtcc ggcctgcttc ttgtcgacgg gaacgagccg gccgccgcca tcgggagtgc
     1381 tgcggaacac gccgagcacg cgcgatttgg cgtggtcgat gaccttgatg acgcgcccgc
     1441 gatagccgac gtcatcgtcg ttgacgcgct cgacccggag caaggcgcgg tcgccgacgc
     1501 cggccatcgt gccggggcgg gcatggcgag gaatatggat gcggatcctc ggcggctcgc
     1561 cctgttcgtc gtcccattcg gcaggggttg cgagcagctc gccgtcagca tcgcgcatcg
     1621 tgatgtcggc gagcacggtt gctggaagct gtgcgatctc ggtgaccttc ttgccgcgtt
     1681 tggtgatggt gccgtggtcg gcgaggtcgc gcaggaggcg cttcaactcg gcgcggtcgg
     1741 cattcttcaa gccgaactcg cgcgcgattt ccttggtacc gaccttgccg ggattcttgc
     1801 ggatgaaagc gacgatggcg tcccgggtcg gaaagccatt gtcgggcttc tgcttcactt
     1861 acctacgtgc ctttccggcg ctcttcttgg cggaggtctt cgccgccgaa ggctttgcag
     1921 ccgaagtctt ggcatccgcc ttgaccgggg cgcgcgcctt gctgacggag tccgatttcg
     1981 acttggccgg tttcttcgcc gaagccttct tcaccgcttt ggcggattcc gcctcgtcgg
     2041 cttccgtctc gtttggctca gctttggcct ttttggtatt ttgggccttg ggtgccttca
     2101 ctgcctgctt tgcagtcttc atggctttcg ccttgccgcc gcccttgttg gttcccttgg
     2161 acgcgcgctc gtcgatcagc gcgatggctt ccgccagcgt gatcgtgtcc ttttccttgt
     2221 cgctcgggat cgtcgcgttg acgccgcccg cggtgacata ggcgccgtaa cggccgctct
     2281 tgacggcgac tgcgccgagc gagggatgat cgccgagctg cttgccggga tcggcgccga
     2341 agcggccacg gctcggaccc ttggcaacct tttctgcgat cagcgtcacg gcgcgattga
     2401 ggccgatatc gaacacctcg tcaccggcct ctagcgaggc ataggtcttg tcgtgcttca
     2461 cgaacggacc aaagcgcccg atgccggcgg tgatcggcag acccgtctcc gggtgcttgc
     2521 cgatctcgcg cggcaacgcc agcagcttca gggcacggtc gagctcgata tcgccgggcg
     2581 aggtgccctt cgggatacct gcgcgcttcg gcttctcgtc ctcgccgtaa tccttctgct
     2641 cgccgagctg gatataaggc ccaaagcggc cggccttgac gttcacgtcg agcccgctct
     2701 cggggtcctg gccgagcagg cggtcggcgc tggcctcggt gtcggcggcg agcggacggg
     2761 tgtagcggca ttccggatag ttcgagcagc cgacaaaggc gccgaacttg ccggccttga
     2821 gattgagctt gccggtgccg caggtcgggc actggcgcac gtcgccgcca tccgcgcgct
     2881 gcgggtagat gtgcgggccc agcatgttgt cgagcacgtc gagcacttcg gcgacgcgaa
     2941 tatccttgat gtcgtcaacc gcgccgatga agtcgcgcca gaaatccttc agcacctgct
     3001 gccaggaaat ctcgttattg gagatccgat cgagctgctc ttccaggtca gcggtgaaat
     3061 cgtattcgac atagcggcgg aagaaatttt caaggaatgc ga
//
LOCUS       DATNAE010000165         4649 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_159437, whole genome shotgun sequence.
ACCESSION   DATNAE010000165 DATNAE010000000
VERSION     DATNAE010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4649
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159437"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1076
                     /locus_tag="VN518_03630"
     CDS             <1..1076
                     /locus_tag="VN518_03630"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018327908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HXE03696.1"
                     /translation="SDRWRGFADVADSRHLANRVEKEVVDALVAAVREAYPRLSHRYY
                     ALKAKWLGRKRLAYWDRNAPLPEEPTQSIGWDDAQKTVLKAYGDFSPRMAAVANEFFA
                     KRWIDAPVREGKSPGAFSHPTVPSAHPYILLNYQGKPRDVMTLAHELGHGVHQVLANA
                     QGPLMAPTPLTLAETASVFGEMLTFRALLERAPSKRERKALLASKVEDMINTVVRQIA
                     FYSFERKVHTERREGELTAERLGEIWLEVQSESLGPAIDLKTGYENFWVYIPHFIHSP
                     FYVYAYAFGDCLVNSLYAVYEKADKGFADRYLDMLAAGGTKHHRELLAPFGLDATDPH
                     FWSMGLKVVEGLIDELEAMDKVS"
     gene            1162..1386
                     /locus_tag="VN518_03635"
     CDS             1162..1386
                     /locus_tag="VN518_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HXE03697.1"
                     /translation="MKIVIKRIGNSEGLILPRELMRRLDLKHGQQLHITELPGGGFQA
                     LPYDPDFEKTMEIVDEIIDEYRDTLAALAK"
     gene            1383..1769
                     /locus_tag="VN518_03640"
     CDS             1383..1769
                     /locus_tag="VN518_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018044346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HXE03698.1"
                     /translation="MTEPKWFTYEQAIAAHSLQLRRFGGALGLRDEGLLRSALGRPRN
                     KWHYEGAELPELAAAYAFGLIKNHAFVDGNKRIAFIGMVAFLRMNGVAFKPDQAQSTA
                     IVLAVAAGEVSEQSLARWIRDNWPRS"
     gene            complement(1782..2231)
                     /locus_tag="VN518_03645"
     CDS             complement(1782..2231)
                     /locus_tag="VN518_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF411 domain-containing protein"
                     /protein_id="HXE03699.1"
                     /translation="MTMRILLLLSLMIFGAAPASAADTAASIEVWKSATCECCGAWVK
                     HLEANGFTVKVNAAEPSALASLKRQAGIGDKLASCHTAMIDGYVIEGHVPGTDIKRLV
                     AERPQAIGLAVPGMPVGSPGMEQGAEFEPYDVLLIKKDGTTETFARH"
     gene            2356..3732
                     /locus_tag="VN518_03650"
     CDS             2356..3732
                     /locus_tag="VN518_03650"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009467626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HXE03700.1"
                     /translation="MTSDKERNRLTARVQRYGRVGANVGGVAARIASTRLLGLDPDRD
                     GEASALAAALGGLKGPIMKVAQLLATIPEALPAEYAAELGQLQSQAPPMGWPFVRRRM
                     AAELGPDWESRFASFEREAAASASLGQVHRAHAHDGRSLAAKLQYPDMQSAVEADLKQ
                     LALLFIIHRQLRPAIETSEMKEEIAARLREELDYDLEAGHMRLYATIFAGDPLIRVPE
                     GLPELSTQRLLTMTWLEGRRLLDYTDRPLEERNQIARAMFRAWWYPFSHYGVIHGDPH
                     LGNYAVFEDEAGKAAGINLLDYGCMRTFAPKFIQGVIDLYRGLLNNDRSLVVHAYETW
                     GFAGLSSELIDILNIWANFIYGPMLEDRVRTIAEDRNIGMYGRREAFKVHQGLRDKGP
                     VKVPREFVFMDRAAIGLGGVFLHLNAELNFHRLFDDTIAGVDLGDVARRQKEAFTAAG
                     VPLPDASV"
     gene            3815..3958
                     /locus_tag="VN518_03655"
     CDS             3815..3958
                     /locus_tag="VN518_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aa3-type cytochrome c oxidase subunit IV"
                     /protein_id="HXE03701.1"
                     /translation="MHIDPAECHPAMDYTEHRKTYRLFLKLALCTIVGVALLMALMAV
                     FVV"
     gene            4036..>4649
                     /locus_tag="VN518_03660"
     CDS             4036..>4649
                     /locus_tag="VN518_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit alpha"
                     /protein_id="HXE03702.1"
                     /translation="MITIGVPAEGPDEPRIGLSPETAKKLVKLGAKVLIRSGAGLRSH
                     FSDQEYKDSGAEIVGTDADAVKDADIVLTVRRPPLDLVKSMKQGAMLIAMLDPYSDAD
                     GLQALAKTGASLFAMELMPRISRAQIMDVLSSQANLAGYKAVVDAAALFERALPMMMT
                     AAGTVPAARVFVMGVGVAGLQAIATARRLGAIVTATDVRPAVKE"
BASE COUNT          849 a         1480 c         1474 g          846 t
ORIGIN      
        1 tctcggaccg ctggcgcggt ttcgccgatg tcgccgatag ccgtcacctc gccaaccggg
       61 tggagaagga ggtggtcgac gcgctggtcg cggccgtacg cgaggcttat ccgcgcctct
      121 cccaccgcta ctatgcgttg aaagccaaat ggctcggcag gaagcggctc gcttattggg
      181 accgaaacgc accgttgccc gaggagccga cgcagagcat cggctgggac gacgcgcaaa
      241 agaccgtgct caaggcctat ggcgatttct cgccgcgcat ggcggcggtg gcgaacgagt
      301 tcttcgccaa gcgttggatc gatgcgccgg tgcgcgaggg caagtctccc ggcgcgtttt
      361 cgcatcccac ggtgccgagc gcgcacccct atatcctgct caactatcag ggcaaaccgc
      421 gggacgtgat gacgctggcg cacgagcttg gtcacggcgt gcatcaggtg ctcgccaacg
      481 cgcaaggccc gttgatggcg cctacgcctt tgacgcttgc ggagacggcg agcgtgttcg
      541 gcgagatgct gaccttccgc gcgctgctcg agcgcgcgcc gagcaagcgc gagaggaagg
      601 cgctgctcgc ctcaaaggtc gaggacatga tcaacacggt tgtgcgccag atcgcgttct
      661 acagcttcga gcgcaaggtg cacacggaac ggcgcgaagg cgagctcacg gctgagcggc
      721 tcggcgagat ttggctcgag gtgcaaagcg agagcctcgg gccggccatc gatctcaaga
      781 ccggctacga aaatttctgg gtctacatcc cgcacttcat ccattcgccg ttctacgtct
      841 atgcctatgc cttcggcgat tgcctggtga actcgctcta tgcggtctac gagaaggccg
      901 acaagggctt cgccgaccgc tatctcgata tgctcgcggc tggcggcacc aagcatcaca
      961 gggagctgct cgcgcccttc ggcctcgacg ccaccgaccc tcatttctgg tccatggggc
     1021 tcaaggtcgt ggaaggcctg atcgacgagc tcgaggcgat ggacaaggtg tcctagtccc
     1081 ttggaattct cgcccgacac cctgttgtac aaaacggcaa acgctgttat acatcgcgta
     1141 taacaacgta tgagacgagc catgaagatc gtgatcaagc gaatcgggaa ttccgagggg
     1201 ctgattttgc cgagggagtt gatgcgccgc ctcgacctca agcatggcca gcaactgcac
     1261 attaccgaac tgccgggcgg cgggtttcag gcgctgccct atgatcccga cttcgagaag
     1321 acgatggaga tcgtcgacga gatcatcgac gagtatcgcg acacgctcgc tgcgctcgcg
     1381 aagtgaccga gccgaagtgg ttcacctacg aacaagcgat tgcagcgcat agcctgcagc
     1441 ttcgccggtt tggcggggca ctaggcttgc gcgacgaagg cctgttgcgc tcggctttgg
     1501 ggcggcctcg caacaagtgg cactatgaag gtgccgaact gcccgagctc gctgccgctt
     1561 acgcgttcgg gctcatcaag aatcacgcgt tcgtcgacgg caacaagcgc atcgcattca
     1621 tcggcatggt cgcgtttctt cgcatgaacg gagttgcgtt caagccggac caagctcaat
     1681 ccaccgcgat cgttctcgcg gttgccgcgg gcgaggtgag cgagcaaagt cttgcgcgtt
     1741 ggattcgaga caattggccg cgttcgtaac gcctgacgcg ctcagtgcct ggcgaaggtt
     1801 tcggtggtgc cgtccttctt gatcagcagc acgtcgtagg gctcgaactc ggcgccttgc
     1861 tccatgccgg gagagccgac aggcatgccg gggacggcga ggccgatcgc ttgcggtcgc
     1921 tcggcaacga ggcgcttgat gtcggtcccc ggcacatggc cttcgatgac atagccgtcg
     1981 atcatggcgg tgtgacagga ggcgagcttg tcgccaatgc cggcttgccg cttcagcgaa
     2041 gcgagcgcgc ttggctccgc cgcgttcact ttcacggtaa agccgttggc ctcaagatgc
     2101 ttgacccagg cgccgcaaca ttcgcaggtc gccgatttcc acacctcgat tgaggcggcc
     2161 gtgtcggcgg cgcttgctgg cgcagcgccg aagatcatca aagacaaaag gagcaggatg
     2221 cgcatcgtca tggttcagtc ctcccgcaga tcagcatgga cgccataagc cgcgtcagca
     2281 attgacaatt tcgtcaatgt caaggtgacg ctaacaccct ggcaatttcc tcagcccgcc
     2341 ttacctttgg gatcaatgac ctccgacaaa gaacgcaatc gcctgaccgc ccgcgtgcaa
     2401 cgctacgggc gcgtcggcgc caatgtcggc ggcgtcgcgg cccgcatcgc gagcacccgt
     2461 ctgcttgggc tcgatccgga tcgcgacggc gaggcctcgg cccttgccgc ggcccttggc
     2521 ggcctcaagg ggccgatcat gaaggtggcg cagctcctcg ccaccatccc cgaggcgttg
     2581 ccggcagagt atgccgccga gctcggtcag ctgcagagcc aggcgccgcc catgggatgg
     2641 cccttcgtgc gccggcgcat ggcggcggag ctggggcctg attgggaaag ccgctttgcg
     2701 agcttcgaac gtgaagcggc ggcgtcggcc tcgctcgggc aagtgcaccg ggcgcatgcc
     2761 catgacggcc ggtccttggc cgcgaagctg caatatcccg acatgcagtc ggcggtggag
     2821 gctgatctca agcagctcgc tttgttgttc atcatccatc ggcagctcag gccggcgatc
     2881 gagacctcag agatgaagga ggagatcgcg gcacgcttgc gcgaagagct cgactacgat
     2941 ctcgaagccg gccatatgcg cctctacgcc acgatctttg ccggcgatcc tttgatccgc
     3001 gtgcctgagg gcctacctga gctttccacg cagaggctgc tcaccatgac ctggctcgag
     3061 gggcgacggc tcctcgacta caccgataga ccgctcgaag agcggaacca aatcgcccgc
     3121 gccatgttcc gcgcctggtg gtatccgttc agccattacg gcgtgatcca cggcgacccg
     3181 catctcggca attacgcggt gttcgaggac gaggcgggga aggcggcggg catcaacttg
     3241 cttgactatg gctgcatgcg caccttcgcg ccgaaattca tccaaggggt gatcgatctt
     3301 tatcgcgggc tgctcaacaa cgaccgctcc ctcgtggtgc acgcgtacga gacctggggc
     3361 ttcgccggcc tctccagcga gctgatcgac atcctcaata tctgggcgaa cttcatctat
     3421 ggccccatgc tcgaggatcg cgtgcgcacc atcgccgagg accgaaatat cggcatgtat
     3481 gggcggcgcg aggccttcaa ggtgcatcag ggcttgcgcg acaaggggcc ggtcaaggtg
     3541 ccgcgcgagt tcgtgttcat ggaccgcgcc gcaatcggcc ttggcggggt attcctgcac
     3601 ctcaatgccg agctgaactt ccatcgcctg ttcgacgaca ccattgccgg cgtcgatctc
     3661 ggcgacgtgg cgcgccggca gaaggaagcc ttcaccgccg ccggggtgcc gcttccggac
     3721 gcatcggttt agtgacgcgc cggattgccc gggccactgc ctttatggta tcggcaagcg
     3781 gaataagggc ctcgaacagc ataaggggag cggaatgcat atcgacccag ccgaatgcca
     3841 tccggcgatg gactacacag agcatcgaaa gacctacaga ctgtttctca agcttgcgct
     3901 ttgcaccatc gtcggcgttg ccctcctcat ggcgctgatg gccgtgttcg tggtctgacc
     3961 cggctcagac ctcccccaaa ggcggcaatc ccgccggcgg ccatttgaca cggcgagtcc
     4021 gctgacggag ccaagatgat caccattggc gtgcccgccg aaggccctga cgagccgcgc
     4081 atcgggttga gccccgagac cgcgaagaag ctcgtcaagc ttggggcaaa ggtgctgatt
     4141 cgctccggcg ccgggcttcg ctcgcatttc tccgaccaag agtacaaaga ttccggcgcc
     4201 gagatcgtcg gcaccgacgc cgacgcggtc aaggacgccg atatcgtgct caccgtgcgc
     4261 cggccgccgc tcgatctcgt caagtcgatg aagcaaggcg ccatgctgat cgccatgctc
     4321 gatccttatt ccgatgccga cggcttgcag gcgctggcca agaccggagc ctcgctgttc
     4381 gccatggagc tgatgccgcg catcagccgg gcgcagatca tggacgtgct gtcgagccag
     4441 gccaatcttg ccggctacaa ggccgtcgtc gatgccgcgg ccctcttcga gcgggcactg
     4501 ccgatgatga tgaccgccgc cggcaccgtc cccgcggcgc gagtgttcgt catgggcgtc
     4561 ggcgtcgccg gcctgcaagc catcgccacg gcgcggcggc tcggcgccat cgtcaccgcc
     4621 accgacgtgc ggcctgcggt caaggagca
//
LOCUS       DATNAE010000166         5037 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2874568, whole genome shotgun sequence.
ACCESSION   DATNAE010000166 DATNAE010000000
VERSION     DATNAE010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5037
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2874568"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            800..2191
                     /gene="ahcY"
                     /locus_tag="VN518_03665"
     CDS             800..2191
                     /gene="ahcY"
                     /locus_tag="VN518_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HXE03703.1"
                     /translation="MNYHVKDIGLASWGRKEIDIAETEMPGLMAVREEYAAKKPLKGA
                     RIAGSLHMTIQTAVLIETLKALGADIRWASCNIYSTQDHAAAAIAAAGVPVFAFKGES
                     LEDYWEYTHAIFEWSDGGTPNMILDDGGDATLLVHLGVRAEKGDTAFLDHATNEEEEV
                     LFAAIKKRLKTKPGWYSEIAKNVRGVTEETTTGVHRLYEMQKKGTLLFPAINVNDSVT
                     KSKFDNLYGCRESLVDGIRRGTDVMMAGKVAMVAGFGDVGKGSAASLRNAGCRVLVAE
                     VDPICALQAAMEGYEVTTMDDASPRADIFVTATGNKDVITIDHMRGMKDRAIVCNIGH
                     FDSEIQVAALRNMKWENVKPQVDEIEFPDGHRIILLSEGRLVNLGNAMGHPSFVMSAS
                     FTNQTLAQIELWANPGTYKKEVYTLPKQLDEKVARLHLGKVGAKLTKLTDEQADYIGV
                     PAEGPYKPDYYRY"
     gene            2422..4665
                     /locus_tag="VN518_03670"
     CDS             2422..4665
                     /locus_tag="VN518_03670"
                     /inference="COORDINATES: protein motif:HMM:NF024267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HXE03704.1"
                     /translation="MRTRSEAAPLLKRALARLGLSLAVVIALPVAAQAADPLVPLSAV
                     YDTRMLLTLGITLGIIAFSIGSAIACLRATQAALRARDAANLEAERYRASESMLETIL
                     SAEPQVLLTWTAEAGPRVHAASLPHGLGVPPEPNRLLRFEEWLDEASAAELTDSMQAL
                     ADRGEAFNLMLRTQRQRHVEVDGRAAGGMIMLKVRDVAGQRLELAGLGEQHRKLDTEV
                     ASLRALLDAQTKAGDRRDAKGRASVETRFRSFDRLATAFAVFDADQRLAHFNQAYVDL
                     WQLDPKLLATRPRDGEILDRLRQSRALPEKADYRDWKKGWLSAYGTNSQREDQWHLPD
                     GRTLHVVADSAAEGGVTYLYENVTERLALESRYNALIHVQRETLDTLREGVAVFAPNG
                     RLRLHNRAFAALWRLSPALLGSEPHIDDIIFACRPLYDDAQEWERTKSAVTAIFSERR
                     PYENQFDRPDGTVITAAALPLPDGGTLITYSDVTDSKRVERALIERNEALETASRVKS
                     AFVSHVSYELRTPLTNIIGFTELLASPSTGAMGEKQREYLSDIRTSGQTLLAIIDDIL
                     DLATIDAGTFELKLAPVKVRGVIDAAVLGLRERLKQNRIELDIRIEPGIDEFVADGPR
                     VTQILYNLLANAIGFSSPGSRIVLSCAHEDDKLAFSVEDEGCGIPDEYQASVFERFES
                     RANGSGHRGAGLGLSIVKSLVQLHGGEVVLTSTPGRGTKISARLPLAHEAAVEPEPRR
                     YGSSRAG"
     gene            4662..>5037
                     /gene="tsaE"
                     /locus_tag="VN518_03675"
     CDS             4662..>5037
                     /gene="tsaE"
                     /locus_tag="VN518_03675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HXE03705.1"
                     /translation="MSANWSLDGVDLVELDRLAQLLALVLKLGDVIAVSGPLGAGKTS
                     FARFLLARLGVEGEVPSPTFSLVQAYASPRFTVHHCDFYRLEPRELAELGLDDLLPSS
                     VTIVEWPERAGDWLPADRLDIAM"
BASE COUNT          929 a         1670 c         1614 g          824 t
ORIGIN      
        1 taaacggtag gttgctttaa ggtgagaccg gcgaccctag caaggccgga aaacgagcca
       61 cttccctcaa aacggccaat acgagctggc ctcgccgccg aggagacgat cgatcctttc
      121 accatttatc cccgccctcc gcgcccgcgt taaactcctc ccatccctgc cccatgaggg
      181 atgcgtgacc taggaccgtc ctggcatgag agggcaggac tgcggcgcct gaggagaact
      241 gcgaagcgga cctgcggcct cttctgagaa agagtggcct cacgtttcgc ggcgggtgta
      301 acggaccacc tcaggggctc gtctgccccc cgcctgggtt ggaaagccca ggtccatctc
      361 tgccggcagc tcatacgctg cccgcggaag aggcggtaag cgaggggaag gggctcgccg
      421 ctgcggggcg ccgagaggct gcccactaaa aaacggacgc ggccgcttgg cgccccgcat
      481 ccctcgcttt gcgctgatgt agcgaagcga tagcgcattg atggatcgct tcgcgctcct
      541 ctgggcggcg ggacgatttg gcgtgcgctt atccctcccc gcgaggggag ggtggcgcga
      601 cgcgcgatga gcgcgtcgaa acgggtgggg cgaacgcgcg gaaaatggcc cccaccccgc
      661 cgccggttcc tccccgcaag ggggaggtat gaaaagagag ccggcacagc cgaggaggca
      721 ccttgacacc ggcgctccga ggcaagatat aaggagttct ttatatcttt atcaggttgg
      781 tgagcggagc agtaacggca tgaactacca cgtcaaggac attggcctcg cctcctgggg
      841 ccgcaaggaa atcgacatcg ccgagaccga gatgccgggc ctcatggcgg tgcgcgagga
      901 atacgccgcg aagaagccgc tgaaaggcgc gcgcatcgcg ggctcgctgc acatgaccat
      961 ccagaccgcg gtgctgatcg agacgctgaa agcgctcggc gccgacatcc gctgggcctc
     1021 ctgcaacatc tattcgacgc aagaccatgc ggccgcggcc atcgccgccg ccggcgttcc
     1081 ggtgttcgcc ttcaagggcg agagcctcga ggactattgg gagtacaccc acgccatctt
     1141 cgagtggagc gacggcggca cgccgaacat gatcctcgac gacggcggcg acgcgacact
     1201 gctcgtgcat ctcggcgttc gcgccgagaa gggcgacacc gcctttctcg atcacgccac
     1261 caacgaggag gaagaggtgc tgttcgccgc catcaagaag cggctgaaga cgaagcctgg
     1321 ctggtattcg gagatcgcca agaacgtacg cggcgtgacc gaggagacca ccaccggcgt
     1381 gcatcgcctt tacgagatgc agaagaaggg cacgctgctg ttcccggcga tcaacgtcaa
     1441 cgacagcgtc accaagtcga aattcgacaa tctctatggc tgccgcgaga gcctggtcga
     1501 cggcatccgc cgcggcaccg acgtgatgat ggcgggcaag gtggccatgg tcgcgggctt
     1561 cggcgacgtc ggcaagggct cggccgcctc gttgcgcaac gcaggctgcc gcgtgctcgt
     1621 ggctgaggtc gatccgatct gcgcgctgca ggcggcgatg gaaggctacg aggtgaccac
     1681 catggacgac gcctcccccc gcgccgacat cttcgtcacc gccaccggca acaaggacgt
     1741 gatcaccatc gaccacatgc gggggatgaa ggaccgcgcc atcgtctgca atatcggcca
     1801 tttcgattcg gagattcagg tggcggcgtt acgcaacatg aaatgggaga atgtgaagcc
     1861 gcaggtcgac gagatcgaat tccccgacgg ccaccgcatc attctcttgt ccgaggggcg
     1921 gctcgtgaac ctcggcaacg ccatgggcca tccgagcttc gtgatgagcg cctcattcac
     1981 caaccagaca ctcgcccaga tcgagctgtg ggccaacccg ggcacttaca agaaagaggt
     2041 ctacacgctg ccgaagcagc tcgacgagaa ggtggcgagg ctccatctcg gcaaggtcgg
     2101 cgccaagctc accaagctga ccgacgagca ggccgactat atcggcgtgc cggcggaagg
     2161 accgtacaag ccggactatt accggtatta gccccagtcg tgtcaggcca acacgctgcc
     2221 ataagactgt catcgtcgtt gcaatgttgg cgccgaacat acgtgccaca gtgggcaaga
     2281 gtcgccgatg tgtttaatct agggtgattc gccttcaagg ctcgcagggg cacgcgcgcc
     2341 gtcgcccgct caagtctgtg gcagcgttca agcacaatgc agcgcggggg ccatgggcga
     2401 agagtggggg cacgagggac catgcgcacg aggagcgagg ctgcaccgct tttgaagcgg
     2461 gcgctggcgc gccttggctt gagcttggcg gtggtgatcg ccctgccggt tgctgcccag
     2521 gcggccgatc cgctggtgcc gttgagcgcg gtctacgata cccgcatgct gctcacgctc
     2581 ggcatcaccc tcggcatcat cgccttttct atcggcagcg cgatcgcctg cttaagggcc
     2641 acccaggctg cgctcagggc gcgcgacgcg gcgaacctcg aggccgagcg ctatcgggca
     2701 agcgagagca tgctcgagac catcctttcg gccgagccgc aggtgctgtt gacttggacc
     2761 gctgaggccg gcccccgagt gcacgcggcg agcctgccgc acgggcttgg cgtgccgccg
     2821 gagccaaacc gcctgctccg cttcgaggag tggctcgacg aggcgtctgc cgccgagctc
     2881 accgattcca tgcaggcttt ggccgatcgc ggcgaggcct tcaacctcat gctcaggacc
     2941 cagcggcaga ggcatgtcga ggtcgacggc cgcgccgccg gcggcatgat catgctgaag
     3001 gtgcgcgacg ttgccggcca gcggctcgag cttgccggcc ttggcgagca gcaccggaag
     3061 ctcgacaccg aggtcgcctc tctgcgcgct ttgctcgatg cccagaccaa ggcaggcgac
     3121 cggcgcgacg ccaaaggcag ggcctcggtc gagactcggt tccgcagctt cgatcgcctc
     3181 gccacggcct tcgccgtgtt cgacgccgac cagaggctcg cccatttcaa ccaggcctat
     3241 gtcgatctct ggcagctcga tcccaagctg ctcgccacgc gcccgcgcga cggcgagatc
     3301 ctcgaccgct tgcgtcaatc ccgcgccctc cccgagaagg ccgactaccg tgactggaaa
     3361 aagggctggc tctcggccta cggcaccaac tcgcagcggg aggatcagtg gcacctgccg
     3421 gatgggcgca cgctgcatgt cgttgccgac agcgcggcgg aaggcggcgt cacctatctc
     3481 tatgagaacg tcaccgagag gcttgcgctc gagagccgct acaacgcgct gatccatgtg
     3541 caacgtgaga ccctggacac gctgcgcgag ggcgtggcgg tgtttgctcc aaacgggcgc
     3601 ctgcgcctgc acaaccgcgc cttcgccgcg ctctggcgct tgagcccggc gctgctcggc
     3661 agcgagcccc atatcgacga catcattttc gcctgccggc cgctctacga cgacgcgcag
     3721 gaatgggagc gcaccaagtc cgcggtcacc gccatcttct ccgagcgcag gccctatgag
     3781 aaccagttcg accggcctga cggcacggtg atcacggctg cggccttgcc gcttccggac
     3841 ggcggcacgc tcatcaccta tagcgacgtc accgactcta agcgcgtcga gcgcgcgctg
     3901 atcgagcgca acgaggcttt ggagaccgcg agccgggtca agagcgcctt cgtcagccac
     3961 gtctcttacg aactgcgcac gccgctcacc aacatcatcg gcttcaccga gctgttggcg
     4021 agcccgtcga ccggcgccat gggtgaaaag cagcgggaat atctcagcga catccgcacc
     4081 tcgggccaga cgctgctcgc catcatcgac gacatcctcg acctcgccac catcgacgcc
     4141 ggcaccttcg agctcaagct cgcgccggtc aaagtgcgcg gcgtgatcga cgccgcggtg
     4201 ctggggcttc gcgagaggct gaagcagaac cgcatcgagc tcgatatcag gatcgagcct
     4261 ggcatcgatg aattcgtcgc cgacggtcct cgcgtgaccc agatcctgta taatctgctt
     4321 gccaatgcca tcggcttctc aagccctggg agcagaatcg tgctgtcctg tgcccatgaa
     4381 gatgacaagc ttgcctttag cgtcgaggac gaaggctgcg gcattcccga cgagtatcag
     4441 gcctcggtgt tcgaacgctt cgaaagccgc gccaatggat ctggccatcg cggcgccggc
     4501 ctcggcctct ccatcgtcaa gagcctggtc cagctgcacg ggggtgaggt ggtgctgacc
     4561 tcgacgccag ggcgcggcac caagatcagc gcgcgcctgc cgcttgcaca tgaagcagcg
     4621 gtcgagcccg agccgcgccg ctacggatca agccgcgccg gatgagcgca aattggagcc
     4681 ttgacggcgt cgacctcgtc gagctcgacc gcctcgccca gcttcttgct cttgtgctca
     4741 agctcggcga cgtgatcgcg gtgtcaggac cgctcggcgc cggcaagacg agcttcgccc
     4801 gctttctgct cgcacgcctc ggcgtcgagg gcgaggtgcc gagcccgacc ttctcgctgg
     4861 tgcaggcata tgcgagcccg cgcttcaccg tgcatcattg cgatttctac cggctcgaac
     4921 cgcgcgagct tgccgagctc ggcctcgacg atctcttgcc aagctcggtg accatcgtcg
     4981 aatggcccga gcgcgccggc gactggcttc cggctgatcg cctcgacatc gccatgg
//
LOCUS       DATNAE010000167         2238 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_766398, whole genome shotgun sequence.
ACCESSION   DATNAE010000167 DATNAE010000000
VERSION     DATNAE010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766398"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..862)
                     /locus_tag="VN518_03680"
     CDS             complement(<1..862)
                     /locus_tag="VN518_03680"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012453817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HXE03706.1"
                     /translation="MDAKTFDKTRLPSRHVTEGPTRAPHRSYYYAMGLSREQIHQPFV
                     GVATCWNEAAPCNISLMRQAQAVKQGVAAASGTPREFCTITVTDGIAMGHQGMKSSLP
                     SREVIADSVELTMRGHCYDALVGMAGCDKSLPGMMMAMCRLNVPSIFIYGGSILPGSF
                     KGRPVTVQDLFEAVGQFSVGKMSADDLDTLEHVACPSAGACGAQFTANTMACVSEAIG
                     LALPYSAGAPAPYEIRDSFCMAAGEKVMDLLALNIRPRDIVTRKALENAATVVAATGG
                     STNAALHLPAI"
     gene            complement(1147..1914)
                     /locus_tag="VN518_03685"
     CDS             complement(1147..1914)
                     /locus_tag="VN518_03685"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HXE03707.1"
                     /translation="MAPEFGRAWVFAALALAALVGILFALFPQWDLAIAQVFWDPARG
                     RFPYATTALPNRLRDLADWLVWLILFAAGGALLLKLVFPRRKMLMRPTVALFLLASYA
                     LGPGLITNGILKPVWSRARPVSIEQFGGQKHFTPWWQPGGECDRNCSFVSGDASSLTW
                     LLAPASVTPPAIRPVALAGAAVIAVGLSGMRVVFGRHFLTDVVFGGVVSLLVIVLCQR
                     LILRLDDARIEAALERAGLRLRSLAPPRSDGRPEDRI"
     gene            complement(1915..>2238)
                     /locus_tag="VN518_03690"
     CDS             complement(1915..>2238)
                     /locus_tag="VN518_03690"
                     /inference="COORDINATES: protein motif:HMM:NF021941.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HXE03708.1"
                     /translation="TLMRLARLDKIDYGRPQAGAVQIVLDEATLALPLAGIIDIGAEQ
                     KRLKREIDKVGSEIRQLDAKLANEKFVSRAPEHVVEEQRERKSEAEATAAKLAQALKR
                     LEAAL"
BASE COUNT          411 a          710 c          744 g          373 t
ORIGIN      
        1 cgatggccgg aagatgcagc gccgcattgg tcgaaccgcc ggtcgcggcc acaaccgtcg
       61 ccgcattttc aagcgccttg cgggtgacga tgtcgcgcgg gcggatgttc agcgcgagga
      121 ggtccatgac cttctcaccc gccgccatgc agaagctgtc cctgatctcg tagggcgcgg
      181 gcgctccggc cgagtaaggc agcgcgagcc cgatcgcctc tgagacgcac gccatggtgt
      241 tggcggtgaa ctgggcgccg caggcgccgg ccgacggaca ggcgacatgc tcgagcgtgt
      301 cgaggtcgtc ggccgacatc ttgccgaccg aaaattgacc gaccgcctcg aacaggtcct
      361 gcaccgtgac cggccggccc ttgaaggagc cgggaaggat cgagccgcca tagatgaaga
      421 tggacggcac gttcaggcgg cacatcgcca tcatcatgcc gggcaaagac ttgtcgcatc
      481 ccgccatgcc gaccagcgcg tcgtagcaat gaccccgcat ggtgagctcg acggaatcgg
      541 cgatgacctc gcgcgagggc agcgaggact tcatgccctg atggcccatg gcgatgccgt
      601 cggtgacggt gatggtgcag aattcgcggg gcgtgccgct cgccgcggcg accccctgct
      661 tcaccgcttg cgcctggcgc atcagcgaga tgttgcaagg ggcggcttca ttccagcagg
      721 tggcgacgcc gacgaagggc tgatggatct gctcgcgcga cagacccatc gcgtaataat
      781 aggagcgatg gggggcgcgg gtgggcccct cggtcacatg gcggctcggc agccgcgtct
      841 tatcgaacgt cttcgcgtcc atctgctaac gcccagatga ggtgcgggaa atatggcctc
      901 tgcgccaccg ttccccaacg gttcggccca actttctccc ggctgtcccc cgatgtggag
      961 ccgtggaaag cgttctagac cgacgcgcct cctacagacg tcgatttctc aaagttttat
     1021 ggcgcaaacg aggcataaat gcctcagctt gttgtaattt agctacagtt gcagagattt
     1081 tggatgaaga tgcaacgccg ggcccaagct ttgcccgtga attaggcgct cgttgcgcca
     1141 gccgggttag atccggtctt ctggtctccc atcgctccgc ggtggagcga gcgagcgcag
     1201 cctcaagcct gctctttcaa gggccgcctc gatgcgtgcg tcatcgagcc ggaggatcaa
     1261 gcgctgacac agcacgatga cgagcaggct cacgacgccg ccgaacacca cgtcagtcaa
     1321 gaagtggcgt ccgaagacca cgcgcatccc gctcaacccc acggcgatca cggcggcgcc
     1381 cgcgagtgcg accggtctga tcgcaggagg cgtgacgctc gccggcgcga gcagccaagt
     1441 gagtgaggag gcatcgccgg acacgaaaga gcaattgcgg tcgcactccc cgcccggctg
     1501 ccaccacggg gtgaagtgct tctggccgcc gaactgctcg atggagacgg gcctcgcgcg
     1561 gctccacacc ggcttcaaga tgccgttggt gatcaagccc gggccgagcg cgtaagaggc
     1621 gagaaggaag agcgcgacgg tcgggcgcat cagcatcttc cgtcgcggga acacgagctt
     1681 gagcaggagc gcacccccgg cggcgaagag gatcaaccat acgagccaat cggcaaggtc
     1741 gcggaggcgg ttgggcaacg cggtcgtcgc gtagggaaag cgccctctgg ccgggtccca
     1801 aaagacttgc gcgatagcga ggtcccattg cgggaagagc gcgaacagga tcccaaccaa
     1861 cgcggctagg gccaaagctg cgaacaccca agcccggccg aactccgggg ccacctagag
     1921 tgctgcctcg aggcgcttca aagcctgggc gagcttggcg gcggtcgctt cagcttcgct
     1981 cttccgctcg cgctgctcct cgacgacgtg ctcgggcgcg cggctgacga atttttcgtt
     2041 ggcgagcttg gcgtcgagtt gcctgatctc ggagccgacc ttgtcgatct cgcgcttcag
     2101 gcgtttttgc tcggcaccga tgtcgatgat gccggccagc gggagcgcaa gcgtcgcctc
     2161 gtcgagcacg atctgcaccg cgccggcttg cgggcggccg taatcgattt tatcgaggcg
     2221 ggcgagtctc atcagcgt
//
LOCUS       DATNAE010000168         6293 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_462531, whole genome shotgun sequence.
ACCESSION   DATNAE010000168 DATNAE010000000
VERSION     DATNAE010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6293
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462531"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..1038
                     /locus_tag="VN518_03695"
     CDS             70..1038
                     /locus_tag="VN518_03695"
                     /inference="COORDINATES: protein motif:HMM:NF021506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HXE03709.1"
                     /translation="MAIPFLIGAGAGLVSAALFASAATATSLAGILFYLAPLPICLAG
                     LGWGWIAAAIAALAGMVVVGGMLGLAPGAVFAGAIAMPMAVLCYLALISRPAAALQGQ
                     ASVGLEWYPVGRLVGWASVIAGALAAIMVLTLGYDADSYRDSIKDLLQNSALKELDRD
                     GTLINESTIGGLSSVLARTLPAAFAIVWQSIALFNLWLAGVIVEASGRPLRPWPALDA
                     IELPNAFFLAFTASLLASFLPGLAGLLATGFAGALLFAFVLQGLAVLHAFSRGMPFRG
                     LLLSAVYIGILLLGWVAVAIAILGLAEPILRLRDRAATRGQPPNPD"
     gene            1066..1689
                     /gene="rplI"
                     /locus_tag="VN518_03700"
     CDS             1066..1689
                     /gene="rplI"
                     /locus_tag="VN518_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008190241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HXE03710.1"
                     /translation="MQVILLERIGRLGQMGDVVTVKDGFARNFLLPQGKALRATKANR
                     ERFEKERAQLEARNLELKSEAEAVATKLQGQSFIVLRQAGDSGQLYGSVATRDIAAVV
                     TEGGFSIERRQVLLDRPIKTLGVHDVRIGLHAEVEPHVTINVARSADEAARQARGEQV
                     TGKAMDEADEEALRAKVAAEKLFEEGAGGELAEGAEESAEDEAESAD"
     gene            1824..3197
                     /locus_tag="VN518_03705"
     CDS             1824..3197
                     /locus_tag="VN518_03705"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HXE03711.1"
                     /translation="MFRPLRHALDRIIASGSLRLIDADVCTHDFGDGSGSPIVARIAD
                     RATERLFILNPALAIGEAYMDGRLVMERGTIYDFLELLLSNLDYPSWPRLARLREGLR
                     FVRRGLRQYNPASRAKRNVEHHYDLDDHLYELFLDSDRQYSCAYFEKEGMSLEEAQAA
                     KKRHLAAKLNLRDGLKVLDIGSGWGGLGLYLAKSAHVDVTGVTLSEEQLRTSQERARA
                     EGLAKAVSFELRDYREVQGQFDRIVSVGMFEHVGVVHYRSFFEKIRELLAPNGVALIH
                     SIGRFDGPASTNPFIDKYIFPGGYIPALSEVLPAVERAGLLVTDVEILRLHYALTLRH
                     WRQRFRASWHTAVERYGERFCRMWEFYLAGAETGFRYQNLMVFQVQLTKGQKALPLTR
                     DYVYTSEHDFRARDARFPPQPARRARTLRRSIPLVVGENSSARCSQAAALAVNIVLTT
                     LGLCPMH"
     gene            3182..4669
                     /locus_tag="VN518_03710"
     CDS             3182..4669
                     /locus_tag="VN518_03710"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HXE03712.1"
                     /translation="MSNALIRPLQPAPETPTFRAPPHNFEAEQALLGAILVNNEACDR
                     VSSFLKPEHFFDGLHGRIYDHAAKLIWAGKRATPITLKTFFQGDEPVGDLTVPQYLGR
                     LAANATTIINAEDYGRTIYDLAIRRDLIQIGEGMVNVAYDAPIDAPPAVQIEDTEQKL
                     YELAETGKYGSGFEPFTTALTHAVDMAANAYRRDGGLSGLATGFTDLDGKMGGLQPSD
                     LVIVAGRPSMGKTALATNIAYHVAQSYRRDPDSQAPLDGAVVGFFSLEMSAEQLATRI
                     ISEQAYIPSERIRRGRIESEEFDRIVEVSQELQNLPLYIDQTGGITVAQLAARARRLK
                     RQRDVGLIVVDYLQLLSGSTRRAAEGRVQEVSEITTGLKALAKELHVPVLALSQLSRQ
                     VEQREDKRPQLADLRESGSIEQDADVVLFVFREEYYVERRQPRENTEEHKQWQQEMDL
                     VTGKAEVIIGKQRHGPTGTVRLQFTPEFTRFGNLAAEERLPERLD"
     gene            4708..5787
                     /gene="alr"
                     /locus_tag="VN518_03715"
     CDS             4708..5787
                     /gene="alr"
                     /locus_tag="VN518_03715"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HXE03713.1"
                     /translation="MDLDSLIANYRSLRDLSAPAECAAVVKADAYGLGMAAVAPALAR
                     AGCKTFFVATLGEAEELRGLLPGATIYVLAGLQQDTAPVYRRLELRPVLNSADEIEEW
                     AAFTKSARARLPAAIHIDSGMNRLGLSAAEVEAVAGAKNSLNAFELALVMSHLACSDE
                     PEHAKNEVQRKAFDALRAKLPKAAASVANSAGILLGRAYHYDLVRPGIALYGGKASRA
                     AASRFARVVQLAGRVLQVRDVPPGETVGYGATRTLKRPSRVATLAVGYADGVFRALSV
                     ADNREGLQVHFGSHPAPLIGRVSMDLITVDVTDVPRELARRGAWVELIGTHVAAHEFA
                     AHAGTIDYEVLTNLGTRAARRYTGG"
     gene            5797..>6293
                     /locus_tag="VN518_03720"
     CDS             5797..>6293
                     /locus_tag="VN518_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018260859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HXE03714.1"
                     /translation="MAKVSKAFVCQACGAVTARWSGKCAACGEWNSIIEETAAPSGPP
                     ALVAIRGGKGRAASFEGLGTETRDAPRLPTGMAEFDRVLGGGLVPGSAVLVGGDPGIG
                     KSTLLLQAAAALASSGAPVIYLSGEEAPAQVRMRAARLGLSQAPVALGTETNLANIIA
                     TLSKA"
BASE COUNT         1061 a         2139 c         1929 g         1164 t
ORIGIN      
        1 tctcgccaca ggttgggacg gctcgccgtc cctaaccgct ctcgatgaag agccgggaca
       61 gcgcgaacta tggcaatacc tttcctcatc ggtgccggcg cgggccttgt ttccgccgct
      121 ctgttcgcct cggcggcaac agccacatcg cttgccggca tcctgttcta cctcgccccc
      181 ttgccgatct gtctcgccgg actcggttgg ggctggatag cagcggcgat cgccgcgctc
      241 gccggcatgg ttgtcgtggg cggcatgctt ggattggcgc caggcgccgt cttcgccggc
      301 gccattgcca tgccgatggc agtcctttgc tacctcgctc tgatctctcg ccctgccgcg
      361 gcgctgcaag gccaagcctc agttgggctc gaatggtacc cggtcggccg tctggtgggc
      421 tgggcttcgg tgatcgccgg cgcgcttgcc gcaatcatgg tgctgaccct cggctatgac
      481 gccgattcct atcgcgactc gatcaaggac ctactccaga acagcgcgct taaagagctc
      541 gaccgcgacg ggacgctgat caatgagagc acgatcggag gcttgagcag cgtgcttgcc
      601 cgcaccctgc cggcggcctt tgccatcgtc tggcaaagca tcgccctgtt caatctgtgg
      661 cttgccggcg tgatcgtcga agcgtccggg cgcccgctcc gaccctggcc ggcactcgac
      721 gccatcgagc tgccgaacgc cttctttctt gccttcaccg cgagcctgct cgcctccttt
      781 ttgcctgggc tcgccgggct gctcgccacc ggcttcgccg gcgcgctgct ctttgccttt
      841 gtgcttcaag gtctcgccgt gctgcatgcc ttcagccgcg gcatgccgtt tcgcggcctg
      901 ctgctctccg ccgtctatat cggcattctg cttttgggct gggttgccgt cgccatcgcc
      961 attctcgggc tcgccgagcc catacttcgc ttgcgcgacc gggccgcgac gcgcgggcag
     1021 ccgccaaatc cggattgatc aatcgaactg aacaggagcg tcgaaatgca agtcattctt
     1081 cttgagcgca tcggccgtct cggccaaatg ggcgatgtgg tcaccgtcaa ggacggcttt
     1141 gcccgcaact ttctcttgcc ccagggcaag gcgctgcgcg ccaccaaggc caatcgtgag
     1201 cgcttcgaaa aggagcgcgc gcagctcgag gcgcgcaatc tcgagctgaa atctgaggcc
     1261 gaggccgtcg ccacaaagct gcaagggcaa agcttcatcg tgttgcgcca ggcaggtgat
     1321 tccggccagc tctacggttc ggtggcgacc cgcgacattg cggcggtggt gaccgaaggc
     1381 ggcttctcca tcgagcgccg ccaggtcctg ctcgaccgtc cgatcaagac gctcggcgtg
     1441 cacgacgtgc gcatcgggct tcatgccgag gtcgagccgc atgtgaccat caatgtcgcc
     1501 cgctccgccg acgaggcggc gcgccaggcc cgtggcgagc aggtgaccgg caaggccatg
     1561 gacgaggccg atgaagaggc cctcagagcg aaagttgccg ccgagaaatt gtttgaagag
     1621 ggcgctggcg gcgagctggc tgaaggcgcc gaggagagcg ccgaggatga agcagagtcg
     1681 gcggactgac cccccgcgac acttggggct acggagcctt aaggaacacc gcgggccgct
     1741 tgttgtttta tcgccgtcag gttcgtaaaa tgacagggta agtcactgaa aacccttcct
     1801 ctcggagggt tggtgctggt ccgatgttcc gcccgctccg gcatgctctc gatcggatta
     1861 ttgcgtcagg atctttgcgc ctgatcgatg ccgacgtttg tacccatgat ttcggcgacg
     1921 gaagcggctc tccgatcgtg gcccgtatcg ccgaccgcgc aaccgaaagg ctgttcattc
     1981 tcaacccggc gctcgccatc ggcgaagcct acatggatgg ccgcctcgtc atggagcgag
     2041 gtaccatcta tgatttcctc gagctgcttc tctctaatct cgactatccc tcttggccgc
     2101 gcctggcacg tttgcgcgag ggcctgagat tcgtccggcg cggcttgcgg cagtacaacc
     2161 cggccagccg ggccaagcgc aacgtcgagc accattacga tctcgatgac catctctacg
     2221 agctgttcct cgacagcgac cggcaatatt cctgcgctta tttcgagaag gagggcatgt
     2281 cgctcgagga ggcgcaagcc gccaagaagc gccaccttgc cgccaagctc aatctccgcg
     2341 acggactgaa agttctcgac atcggctccg gctggggcgg ccttggcctc tatctcgcca
     2401 aatccgccca tgtcgacgtg accggagtca ccttgagcga ggagcagctc agaacatcgc
     2461 aagagcgcgc tcgcgccgag ggccttgcca aggccgtcag cttcgagctt cgcgactacc
     2521 gtgaggtgca aggtcagttc gaccgcatcg tctcggtcgg gatgttcgag catgttggag
     2581 tggtccacta tcggagcttc ttcgagaaga tccgcgagct gctcgcgccc aatggcgtcg
     2641 ctcttattca ctcgatcggc cgcttcgacg gacctgcttc gaccaatccc tttatcgaca
     2701 agtatatctt ccctggcggc tacatcccgg cgttgagcga agtgctgccc gcggtcgagc
     2761 gcgccggact tctcgtcacc gatgtggaga tcctgcggct ccactacgcg ctcaccttgc
     2821 gtcactggcg ccagcgcttc cgcgcctcat ggcacacggc ggtggagcgc tacggcgaac
     2881 gcttctgccg catgtgggag ttctatctgg cgggcgccga gaccgggttc cgctaccaga
     2941 acctcatggt gttccaggtg caattgacca agggccagaa agcgctgccg ctcacccgcg
     3001 actacgtcta tacctcggag cacgacttcc gcgcgcggga cgcccgcttc ccgccacagc
     3061 ccgcgcggcg cgccaggacg ttgagacgct cgatcccgct cgttgttggc gaaaacagtt
     3121 ctgcccgctg ttcacaggcc gccgcactag ccgtcaatat cgttttgact actctgggct
     3181 tatgtccaat gcactgattc gcccgctgca gccggcccca gagacgccga ccttccgcgc
     3241 cccgccccat aatttcgagg cggaacaagc gcttctcggc gccatcctgg tcaacaacga
     3301 ggcctgcgac cgggtgtcga gcttcctcaa gcctgagcat ttcttcgatg gtctgcatgg
     3361 gcgcatctac gaccatgcgg caaagctgat ctgggcggga aaacgcgcga cgccaatcac
     3421 gctcaagacc ttcttccagg gagatgagcc cgtcggcgac ctgaccgtcc cgcaatatct
     3481 cggccgtctt gccgccaacg ccaccaccat catcaatgcc gaggattacg gccgtaccat
     3541 ctacgatctc gccatccgca gggatctgat ccagatcggc gagggcatgg tcaacgtggc
     3601 ctatgacgcg ccgatcgacg cgccgcccgc ggtgcagatc gaagacaccg agcagaagct
     3661 ctatgagctt gccgagaccg ggaaatacgg ctcgggcttc gaacccttca ccaccgcgct
     3721 cactcacgcc gtagatatgg cggccaatgc ctatcggcgc gacggaggac tgtctggcct
     3781 tgccaccggc ttcacagacc tcgacggcaa gatgggcggc ctgcagccct cagacttggt
     3841 catcgtcgcc gggcgcccct ccatgggcaa gacggcgctc gccaccaaca ttgcctatca
     3901 cgtggcacag agctaccgcc gcgaccctga ttcgcaggct ccgctcgacg gcgccgtggt
     3961 cggattcttc tccctcgaaa tgtcggctga gcagctcgcc acccgcatca tttccgagca
     4021 ggcctatatt ccctcggagc gcattcgccg cggccgcatc gagtccgagg agttcgaccg
     4081 catcgtcgag gtctcccagg agctgcagaa cctgccactt tatatcgatc agactggagg
     4141 catcaccgtg gcccagcttg ccgcgcgggc tcggcgcctc aagcggcagc gcgacgtcgg
     4201 cctgatcgtc gtcgactatc tccagcttct gtcaggctcg acaaggcgcg ccgctgaagg
     4261 ccgcgtgcag gaagtttccg agatcaccac tggcttgaag gcgctcgcca aggagctgca
     4321 cgttccggta cttgcgctct cccagctgtc acgccaggtt gagcagcgcg aggataagcg
     4381 tccacaactt gcggatttgc gcgaatccgg ctccatcgag caggacgcgg acgtggtgtt
     4441 gttcgtcttc cgcgaggaat attatgtcga gcgcagacag ccgcgcgaga acaccgaaga
     4501 gcacaagcag tggcagcaag agatggacct ggtcaccggc aaggccgaag tgatcatcgg
     4561 caagcagcgc cacgggccca cgggcaccgt gaggctccag ttcacgccgg agttcacgag
     4621 gttcggcaac ctcgccgcgg aggagcgcct gccggagcga ctagactgag cggggtcgat
     4681 gacgaaacgg ccaccgcgct gcttaccatc gatctcgact cgctcatcgc caactaccgc
     4741 agcttgcgcg acctcagcgc gcctgccgaa tgcgccgctg tggtcaaggc cgacgcctat
     4801 gggctgggca tggcagcggt cgctcctgcg ctcgcgcgcg ccggctgcaa gaccttcttc
     4861 gtcgccaccc ttggtgaggc cgaggagctg cgcggactcc tccccggcgc caccatctat
     4921 gtcttggccg ggctgcaaca ggatactgcc cccgtctacc gccggcttga gcttcgcccc
     4981 gtgctcaaca gcgccgacga gatcgaggag tgggcagcgt tcacgaaatc tgcgcgcgcc
     5041 aggctcccag ctgccatcca tatcgacagc ggcatgaacc ggcttggact ctcagccgcc
     5101 gaggtcgagg ccgttgccgg cgcgaagaac tctttgaacg ccttcgagct cgcactggtg
     5161 atgagtcatc tcgcttgctc cgacgagcct gagcacgcca agaacgaggt gcagagaaaa
     5221 gccttcgacg cccttcgcgc caaattgccg aaggcagcgg caagcgtcgc caactccgcc
     5281 ggcattctgc tcgggcgcgc ctatcactac gacctcgtcc gcccggggat cgccctttac
     5341 ggcggcaagg cttcccgcgc cgccgccagc cgatttgccc gcgtggtgca gcttgcggga
     5401 cgcgttctgc aagtgcgcga cgtgccgcct ggagagaccg tcggctacgg tgcgacgcgc
     5461 acgctgaaac gcccctcgcg cgtcgccacc cttgcggtgg gatatgccga cggcgtcttc
     5521 cgtgcgctgt cggttgcgga taaccgggag ggtttgcagg ttcatttcgg atcgcatccg
     5581 gcgccgctca tcggccgcgt gtccatggat ctgatcaccg tggacgtgac cgacgttccc
     5641 cgcgagcttg cccggcgcgg cgcctgggtc gagctgatcg gcactcacgt cgccgcccac
     5701 gaatttgccg ctcatgccgg taccatcgac tacgaagtcc tcaccaatct cggcacgcgc
     5761 gcggcgcggc gctataccgg cggctagcat ctcgtcatgg ccaaagtttc caaagccttc
     5821 gtctgccaag cctgtggcgc ggtgaccgcc cgctggtcgg gcaaatgcgc ggcctgcggg
     5881 gagtggaaca gcatcatcga ggagactgca gcgccgtcag gaccgccggc gctcgtcgcc
     5941 atcagaggcg gcaaaggcag ggctgcctcc tttgaagggc ttggtaccga gacacgcgac
     6001 gcgccgcggc tgccgaccgg catggccgag ttcgaccgcg tgctcggcgg cggtctcgtc
     6061 cccggctccg ccgtgcttgt gggcggcgac cctggcatcg gcaagtccac cctgctgctc
     6121 caggccgccg cggcgctcgc atcaagcggc gcgccggtca tctatctctc gggcgaagaa
     6181 gcgccggcgc aggttcgcat gcgtgccgcg cggcttgggc tcagccaagc ccccgtggcg
     6241 ctcggcaccg agaccaacct cgccaatatc atcgccacct tgagcaaggc gaa
//
LOCUS       DATNAE010000169         3421 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_161466, whole genome shotgun sequence.
ACCESSION   DATNAE010000169 DATNAE010000000
VERSION     DATNAE010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3421
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161466"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="VN518_03725"
     CDS             <1..380
                     /locus_tag="VN518_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HXE03715.1"
                     /translation="SDKDKIVPPIRELAAMGFRIIATRGTKRHLDAHGIECEKVNKVL
                     EGRPHIVDAITNGEVDIVFNTTEGAKALADSFSIRRSALLYHIPYYTTVAGALAVTEA
                     IKALRAGNLTVAPLQSYAGLKPA"
     gene            473..949
                     /gene="greA"
                     /locus_tag="VN518_03730"
     CDS             473..949
                     /gene="greA"
                     /locus_tag="VN518_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HXE03716.1"
                     /translation="MMDKVPMTAEGYAALEAEIKHLKTVERPRIIKAIADARTHGDLS
                     ENAEYHAAKEQQGITEARMADLEDKLSRADIIDVSRLKGDQVMFGATVTLIDEDTDEK
                     VKYRVVGEIEADVKKGKISITSPLARALLGKRKNTIVEVSTPGGGKSYKVLKVEFK"
     gene            1029..1814
                     /locus_tag="VN518_03735"
     CDS             1029..1814
                     /locus_tag="VN518_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03717.1"
                     /translation="MISLNALLGRPVEYYEYPWAIEGRLRSAQSRLRSLFQASDVVFI
                     FSKSMPRSGHRFLTDCLTHYFGPDLHYCGFYRPGCCHRIPCAHPQGDGKASRYFMQKS
                     HDFGFRDSAKLNGKYLIQYRSPIPRLQSNYDLAVARGTVGQSKEDFASFAERESVYFI
                     NFYQKWVAEPRLNALAVAYEDLIGEQARTLAAVVSFVQGDSSVDQAALARALSQAPVG
                     ANGGAGSVRDPMQHAYYDPVLFAQLEHRISKECGKDRIRFHFI"
     gene            complement(1924..2346)
                     /locus_tag="VN518_03740"
     CDS             complement(1924..2346)
                     /locus_tag="VN518_03740"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HXE03718.1"
                     /translation="MVAKTALITGISGQDGAYLARALLARGYRVVGAFRRTSGISVGR
                     LEELGISGEVELVDMELLEASNLRHVLNKLKPDEIYNLAAQSFVGLSFEQPLLRYRRP
                     RRAAPARSHARGEPRRPHPDDGQGRLRTGEDRHPPLLS"
     gene            complement(2348..>3421)
                     /locus_tag="VN518_03745"
     CDS             complement(2348..>3421)
                     /locus_tag="VN518_03745"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXE03719.1"
                     /translation="EALRATARALEAAGVPFTLFGLDEYTTARLQDRSMAKHESPRLG
                     SKANLVCDGLIGADIAVRALRPAAFAGRTNILRPFWELSKVPPRFAESLSRFQEIWAP
                     SEFVRASFAEAVDVPVLRMPVPVELGEIAPVSRAGLGLPEEATLFLFAFDPCSFFVRK
                     NPIAVVRAFHKAFGERGRSDVGLIVKTLDAGPHAGVLKPLKAAIGGDPRIHLVEGTVS
                     RAEMNGLLAAADVFVSLHRSEGFGFGLAEAMLLGKKVIGTDYSGNTDFLNTETGYLVP
                     YQLVPVGAGEYPDHQGQVWAEPDIGVAAAHMASIVDNPAAAQVRALAGQAFIKTHHSL
                     AAVGKQMRDRLAALGLLDSHGGA"
BASE COUNT          652 a         1067 c         1108 g          594 t
ORIGIN      
        1 actcggacaa ggacaagatc gtgccgccga tccgcgagct tgccgccatg ggctttcgca
       61 tcatcgccac ccgcggcacc aagcgccacc tcgacgctca cggcatcgag tgcgaaaagg
      121 ttaacaaagt gttggagggc cggccgcata tcgttgacgc catcaccaac ggcgaggtcg
      181 atatcgtgtt caacaccacg gaaggggcca aggcgctcgc cgattcgttc tcaatccggc
      241 gctcggcctt gctctaccat atcccctatt atacaaccgt ggctggcgct cttgccgtga
      301 ccgaggcgat caaggccctg cgggccggca acctcaccgt agcgcccctg caaagctatg
      361 cgggcctgaa accggcttaa ccgctcgctt agcaggcact ttacccgaat cttgacttag
      421 actgggcaat tcctggacag tatccggctg ggcccagagg agctttgaac tgatgatgga
      481 caaggtgccg atgaccgcgg aaggctatgc agcgctcgag gctgagatca agcatctcaa
      541 gaccgtcgag cgcccgcgca tcatcaaggc aatcgccgat gcgcgcacgc atggagacct
      601 ttccgagaac gccgaatacc acgccgccaa ggagcagcaa ggcatcaccg aggcgcgcat
      661 ggccgacctc gaggacaagc tctccagggc cgacattatc gacgtctcca ggcttaaggg
      721 cgaccaggtg atgttcgggg caaccgtcac cctgatcgac gaggataccg acgaaaaggt
      781 gaagtaccgg gtggtgggcg agatcgaggc cgacgtgaag aagggcaaga tctcgatcac
      841 ctctccgctc gcccgcgctc tcctcggcaa gcgcaagaat accatcgtcg aggtgtcgac
      901 gccgggtggg ggcaagtctt acaaggtgct gaaggtcgag ttcaaatagg ccggtcatac
      961 ccggtccgtt gcacggcgac gctctctgcc attgatttgt cgcgtggcgt cggcgaggag
     1021 cgcttcaagt gattagcctg aacgcgttgc tcggacgccc ggtcgagtac tacgaatacc
     1081 cctgggcgat cgaaggccgt ttgcggagcg cgcagtcgcg gctccgctcg ctgtttcagg
     1141 cgagcgacgt cgtcttcatc ttctcgaagt cgatgccgcg gagcgggcat cgcttcctca
     1201 ccgactgcct cacccactat ttcgggccgg atctgcatta ttgcggcttc taccggccgg
     1261 gatgctgcca ccgcattccc tgcgcccacc cgcaggggga cggaaaggcc agccgctatt
     1321 tcatgcagaa gagccacgat ttcggcttcc gcgattcggc gaagcttaac ggcaagtatc
     1381 tgatccaata ccgctcgccg atcccgcgcc tgcaatcgaa ttacgatctc gcggttgctc
     1441 gcgggacggt cgggcagagc aaggaggact tcgccagctt tgccgaacgc gagtcggtgt
     1501 acttcatcaa cttctaccag aaatgggtgg cggagccgcg gctgaacgcg ctcgccgtcg
     1561 cctatgagga tttgatcggc gagcaggcga ggacgctcgc cgcggtggtg agcttcgtcc
     1621 aaggagattc aagcgtcgac caggcggcgc tcgcgcgggc gctttcgcag gcgccggtcg
     1681 gggccaacgg cggcgccggc agcgtgcgcg acccgatgca gcacgcttat tacgatccgg
     1741 tgctgtttgc gcagctcgag cacaggattt cgaaggaatg cggcaaggac cgcatcaggt
     1801 ttcatttcat ttagctgaga gtttccgtgg cctcgtcacc ccggcgaaag ccggggtcca
     1861 gtttttcttc gcgcgtcccg cgcgagctgg attccggcgt tcgccggaat gacgacccgc
     1921 aagtcagctc agcagcggag gatgccggtc ttcacccgtt cgtagtcggc cttgaccatc
     1981 atctggatga ggccttcgag gctcgcctcg cgcatggctt cgagcaggcg cagcacgccg
     2041 agggcgtcga tatctgagga gcggctgctc gaaggagagg ccgacgaagc tctgcgccgc
     2101 gagattgtag atctcgtcag gcttgagctt gttgagcacg tggcggagat tgcttgcttc
     2161 aagcagctcc atgtcaacca gctccacctc gcccgaaatg ccgagctcct cgagcctacc
     2221 gacactgatg ccggaggtac ggcgaaaggc gccgacgacg cgatagccgc gcgcgagcag
     2281 cgccctggcg agataggctc cgtcttgacc agagatgccg gtgatcagcg cggtctttgc
     2341 caccatctca ggctcccccg tgactatcga ggaggccgag agcggcgagg cggtcgcgca
     2401 tctgcttgcc caccgcggca aggctgtgat gggtcttgat gaaggcctgg ccggcgagtg
     2461 cacgaacctg ggcggcggcc ggattgtcga cgatcgatgc catgtgcgcc gcagcgacgc
     2521 cgatatcggg ctcggcccag acttggcctt ggtgatcggg atattcgccg gcccccaccg
     2581 gtacgagctg gtagggcacg aggtatcccg tctcggtgtt gaggaagtcg gtattgcccg
     2641 aatagtccgt gccgatcacc ttcttgccga gcagcatggc ctcggccagc ccaaaaccga
     2701 agccttccga tcggtgcaac gagacgaaga cgtctgctgc cgcgagcagc ccattcatct
     2761 cggcgcggct cactgtgcct tcgacaagat gaatgcgggg atcgccgccg atcgcggcct
     2821 tcagcggctt gagcacgccg gcatgaggtc cggcatcgag cgtcttgacg atgaggccga
     2881 catcggagcg gccccgctcg ccgaaggctt tatggaaggc gcggaccacg gcgatcgggt
     2941 tcttgcgcac gaagaaggag caagggtcga aggcgaagag gaacagggtc gcctcttcgg
     3001 ggagcccgag gcccgcgcgg ctgaccggcg cgatctcgcc aagctcgacc ggaaccggca
     3061 tgcgcagcac cggaacgtcg acggcttccg cgaaggaagc gcgcacgaac tcagacggcg
     3121 cccagatctc ctgaaagcgc gacaagctct cggcaaagcg cggcggaacc ttggagagct
     3181 cccagaacgg gcggaggata ttggtgcgtc cggcaaacgc ggcaggccgc agcgcgcgga
     3241 ccgcgatatc ggcgccgatc aggccgtcac agacgaggtt cgccttgctg ccaaggcgtg
     3301 ggctctcatg cttggccatg ctgcggtcct gaagccgcgc cgtggtgtat tcgtcgaggc
     3361 cgaacagcgt gaagggcacg ccggcagctt ctagcgcgcg ggccgtggcg cgaagcgctt
     3421 c
//
LOCUS       DATNAE010000170         2562 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_4071692, whole genome shotgun sequence.
ACCESSION   DATNAE010000170 DATNAE010000000
VERSION     DATNAE010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4071692"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..918)
                     /gene="rpoH"
                     /locus_tag="VN518_03750"
     CDS             complement(22..918)
                     /gene="rpoH"
                     /locus_tag="VN518_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HXE03720.1"
                     /translation="MAARSLPSVGAHGGLTRYLEEIRQFPMLEPQQEYMLAKSWREHD
                     DRDAAHQLVTSHLRLVARIAMGYRGYGLPIGEVISEGNVGLMQAVKRFDPDKGFRLAT
                     YAMWWIRAAIQEYILRSWSLVKMGTTAAQKKLFFNLRKIKGQMKALEEGDLRPDQVKR
                     IATQLGVTDEDVISMNRRLAGDSSLNAPVRNDAESGEWQDWLVDDTMDQETALAESEE
                     RDQRRGMLNDALKGLNARERRVFEARRLAEEPLTLEELSAEFGVSRERIRQIEVRAFE
                     KVQKAVTKAAAAAAQPEPAGAE"
     gene            complement(1030..2043)
                     /locus_tag="VN518_03755"
     CDS             complement(1030..2043)
                     /locus_tag="VN518_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019965080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HXE03721.1"
                     /translation="MPQSKKTLPLKRLAAEADAGAEGQRLDRFLATQLPELSRTRVQS
                     LIKEGQVSLGGATIVEAKYRVKPGDRFELGVPPAAPPVPTGEAIPLHIAYEDDALIVI
                     DKPAGLVVHPGAGHATGTLVNALIAHCGASLSGIGGVARPGIVHRLDKDTSGLLVVAK
                     TDQAHRALAKQFADHGRTGELERGYLALVWGAPSRPHGTIDAPVGRHPASRTKMAVLS
                     GKGREAVTHWRVVETFGRGKEPIASLIECTLETGRTHQVRVHLAHMGHPLIGDPLYGQ
                     GFKSKLRTLPEPLQGKLAGLDRQALHAEHLAFAHPKSGTLLKFNSPLPADLAEIVAAF
                     KEL"
     gene            2056..2415
                     /locus_tag="VN518_03760"
     CDS             2056..2415
                     /locus_tag="VN518_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03722.1"
                     /translation="MAVASEPDNTPLPGTVFTPRQVRVLKFAVIVMGILLVGGFAFVL
                     AAIVYQASRGGQSSAAPGGEPVATELQIPKDASVSTLSLDGDRLALQLQSSAGPEIVV
                     IDLGTGKTLARIKLKPE"
BASE COUNT          432 a          848 c          814 g          468 t
ORIGIN      
        1 caagcccggc atgacgggag attactcggc gccggcaggc tcgggctgtg ccgcggcggc
       61 ggccgccttg gtcaccgcct tctgcacctt ctcgaaagcg cgcacctcga tctgacggat
      121 gcgctcgcgg gagacgccga actcggcgga aagctcctcc agcgtcagcg gctcctcggc
      181 aagccggcgc gcctcgaaca cacgacgctc gcgcgcattc aagcccttca gcgcgtcgtt
      241 caacatgccg cggcgctggt cgcgctcctc ggattcggca agcgccgtct cctggtccat
      301 ggtgtcgtcg accagccagt cctgccactc gccgctctcg gcatcgttcc tgaccggggc
      361 gttgagcgat gaatcgccgg cgagccggcg gttcatcgag atcacatcct cgtcggtcac
      421 gccgagttgg gtggcgatgc gcttcacctg atccggtctg aggtcgccct cttcgagcgc
      481 cttcatctga cccttgatct tgcgcagatt gaaaaacagc ttcttctgcg cggcggtggt
      541 gcccatcttc accaggctcc acgaacgcag gatgtattcc tggatcgccg ccctgatcca
      601 ccacatggcg taggtcgcaa gccggaaacc tttgtcggga tcgaagcgct tcaccgcctg
      661 catcaggccg acattgcctt cggagatcac ttcgccgatc ggcaacccat agccgcgata
      721 gcccatggcg atgcgcgcga cgaggcgcag atgcgaggta acaagctgat gcgcggcgtc
      781 gcggtcatca tgctcgcgcc agctcttggc gagcatgtat tcctgctgcg gctccagcat
      841 cggaaattgt ctgatttctt cgaggtagcg cgtcagtcca ccatgcgcgc caacactcgg
      901 caaacttctt gcggccatga gagcccgtct ccagcggcct cgaacggccg cgatctaccc
      961 aaaccaaccg ccccccggcg agtcctctgt ataaatcctt tgtcacagag ataaaagcct
     1021 aacacctgtt cacagttcct tgaaagcggc cacgatctcg gcgagatcgg cagggagcgg
     1081 actattaaat ttgagtaatg tgccgctctt cgggtgcgcg aaggcgagat gctcggcatg
     1141 cagcgcctga cggtcgagcc cggcaagctt gccttgcaga ggctcgggca gcgtgcgcag
     1201 cttggatttg aagccctggc cgtacagcgg atcgccgatc agcgggtgac ccatatgcgc
     1261 cagatgcacg cgcacctggt gggtgcggcc cgtttcgagc gtgcactcga tcagcgaggc
     1321 gatcggctcc ttgccgcggc cgaacgtctc aaccacgcgc caatgcgtca cagcctcacg
     1381 acccttgccg gacagcactg ccatcttggt gcggcttgcg ggatgccggc cgacaggcgc
     1441 atcgatggtt ccgtgtggcc gcgatggcgc gccccacacc aaagcgagat agccgcgctc
     1501 gagctcgccg gtgcgcccat gatcggcgaa ttgcttggcg agcgcgcgat gcgcttgatc
     1561 ggtcttggcc accaccaaca gcccgctggt gtccttgtcg aggcgatgca cgatgccggg
     1621 ccgcgccacg ccgccgatgc cggagaggct cgcgccgcaa tgggcgatca gcgcattgac
     1681 cagcgtgccg gtggcgtggc cggcgccggg atgcaccacg aggcccgcgg gcttgtcgat
     1741 gacgatgagc gcgtcgtcct cgtaggcgat gtggaggggg atggcctcgc ctgtgggcac
     1801 tggcggcgcg gccggcggca cgccaagttc gaagcggtca cccggtttga cccggtattt
     1861 cgcctccact atggtcgccc cgcccaagga aacttggccc tctttgatca ggctctgcac
     1921 gcgggtgcgg ctcagctcag gcagctgcgt cgcgaggaag cggtcgagcc gctgcccttc
     1981 ggcgccggca tcggcttccg cggcgagccg cttgagggga agtgtcttct tcgattgagg
     2041 catgaggtgt tgaggatggc ggtcgcgagc gagcccgaca atacgccatt gccaggcacg
     2101 gtgttcaccc cgcgtcaggt gcgggtgctg aaatttgccg tcatcgtgat gggaatcttg
     2161 ctggtcgggg gctttgcctt cgtcctggcg gccattgtct atcaggcctc acggggagga
     2221 caaagcagcg ccgctccagg cggcgagccc gtcgccaccg agctgcagat tcccaaggat
     2281 gcgagcgtca gcacgctttc ccttgacggc gaccggctcg ctctccaatt gcagtcgagc
     2341 gccggaccgg agattgtcgt gatcgacctc ggcaccggca agacgctggc ccgcatcaag
     2401 ctgaagcccg aataagcgct caaacagcga agcggtggcg cagcaataac cccttgatcg
     2461 ccttcgccgg gccaagctat agctttcgct cgatctcggc ccggagcaca cgccccgttc
     2521 gtctagtggc ctaggacgct ggcctctcac gccggaaaca gg
//
LOCUS       DATNAE010000171         2734 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2569286, whole genome shotgun sequence.
ACCESSION   DATNAE010000171 DATNAE010000000
VERSION     DATNAE010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2569286"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1341
                     /gene="purF"
                     /locus_tag="VN518_03765"
     CDS             <1..1341
                     /gene="purF"
                     /locus_tag="VN518_03765"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HXE03723.1"
                     /translation="QHRGQEAAGIVSFDGAHFHTERRLGLVGDHFTASQVIERLKGDA
                     AIGHVRYSTCGDPIMRNVQPLFVDLASGGFALSHNGNLTNALTLRDWLITKGAICQST
                     TDTEVILHLVARSEKRLFVEKFIEALLQVEGAYALVGLTNKKLVGARDPFGIRPLVLG
                     KLGEAWVLASETCALDIIGADFVREIENGEVVVITKDGIESHRFVPKYPARLCIFEYI
                     YFARPDSIVGGRSVYDVRKEMGRQLALEAPANADVVIPIPDSGVPAAIGYSQGSGVPF
                     EFGIIRNHYVGRTFIEPEQRIRQLGVKLKHSANEAQVRGKRIVLIDDSVVRGTTSVKI
                     VQMMREAGAKEVHMRISSPPITHPDFYGIDTPDQDKLLAATKTLEQMRTYMGADTLAF
                     LSVEGIYKAMGYEGRDAAHPQFTDHCFTGEYPTRLRDREGPPQQKQLSFLAEVG"
     gene            1338..2051
                     /locus_tag="VN518_03770"
     CDS             1338..2051
                     /locus_tag="VN518_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019905384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXE03724.1"
                     /translation="MTGQRLKDRIALVTGASRGLGRALALAFAREGAHVLLLARGMTN
                     LGRVDDDIRAIGGSASLVPLDLTDGQAIDALGPSLYERFGRLDVLVGNAAILGALSPL
                     PHIPSAHWDRVFAVNVTANWRLIRTLDPLFRRSDAARVIFVTSGVARSARAYWAPYSV
                     SKAALKALARTYANETADTPIRVNLVDPGALATRMRAEAYPGEDQATLPTPEQVTEIF
                     VTLAMAENDVTGQLFTPEV"
     gene            2088..>2734
                     /locus_tag="VN518_03775"
     CDS             2088..>2734
                     /locus_tag="VN518_03775"
                     /inference="COORDINATES: protein motif:HMM:NF015737.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HXE03725.1"
                     /translation="MAGLPQAGGSTAIAKQGMPAMRHAGAAFAALLIAPFPGAAAELC
                     PALSSTTPTPCESLIDPASDLLQNRINSRLNVVGATPDGESVWAESRQAPFQTVGGAS
                     ALTTTYFGADYRLGSDLLLGAMVQRDDRIVTLPIDGEVTATDAYLAGPYAAYRLSSNL
                     VLGARASWGKASDGWTLASEEANFTTDRLLTEARLSGSWGLGEWQLMPAAAVTHL"
BASE COUNT          479 a          937 c          815 g          503 t
ORIGIN      
        1 caacatcgcg gccaggaagc cgccggcatc gtcagcttcg acggcgccca tttccacacc
       61 gagcggcgct tagggctggt cggcgaccac ttcaccgcaa gccaggtgat cgagcgcctg
      121 aagggcgacg cggccatcgg ccatgtgcgt tattcgacct gcggcgaccc gatcatgcgc
      181 aacgtgcagc cgctgttcgt cgacctcgcc tcaggcggct ttgcgctttc ccacaacggc
      241 aatctcacca acgcgctcac gcttcgcgac tggctaatca ccaagggtgc catctgccaa
      301 tcgaccaccg acacggaagt gattctccat cttgtcgccc gcagcgagaa acgcctgttc
      361 gtggagaagt tcatcgaggc gttgctgcag gtcgaaggtg cctatgcgct ggtcgggctg
      421 actaacaaaa aactcgtcgg cgcccgcgat cctttcggca tcaggccgct cgtgcttggc
      481 aagcttggcg aggcttgggt gctcgcttcc gagacttgcg cgctcgacat catcggcgcc
      541 gatttcgtgc gcgagatcga gaacggcgaa gtggtggtga tcaccaagga tgggatcgag
      601 agccaccgtt tcgtgcccaa atacccggcg cgcctctgca tcttcgaata catctatttc
      661 gccaggccag attccatcgt cggcggccgc tccgtctacg acgtccgcaa agagatgggc
      721 cggcaattgg cgctggaagc tccggccaac gccgacgtgg tcatccccat tcccgattca
      781 ggcgtgccgg ctgccatcgg ctattcgcaa ggctccggcg tccccttcga attcggcatc
      841 atccgcaacc actatgtcgg acgcactttc atcgagccgg agcagcgcat ccgccagctc
      901 ggcgtgaagc tcaagcacag cgccaacgag gcgcaagtgc gcggcaagcg catcgtgctg
      961 atcgacgata gcgtggtgcg cggcacgacc tcggtgaaga tcgtgcagat gatgcgcgag
     1021 gccggcgcca aggaagtgca tatgcgcatt tcgagcccgc cgatcaccca tccagacttt
     1081 tacggcatcg acacgccgga tcaggacaag ctgctcgccg ccaccaagac tcttgagcag
     1141 atgcgcacct atatgggcgc cgacacgctc gccttccttt cggtcgaagg catctacaag
     1201 gccatgggct atgaggggcg cgatgcagcc catccgcagt tcaccgacca ttgcttcacc
     1261 ggcgaatatc cgaccaggct cagggaccgt gaaggtccgc cgcagcagaa gcagctctcc
     1321 ttcctcgccg aggtcggttg accggccagc gtctcaagga ccgcatcgcg ctcgttaccg
     1381 gcgcttcccg cgggcttggc cgtgcccttg ctctcgcttt cgcccgcgaa ggggcgcatg
     1441 tgctgctgct cgcccgtggc atgaccaatc ttgggcgggt cgatgacgac atccgcgcga
     1501 tcggcggcag cgcgagcctc gttccgctcg atctcaccga cgggcaagcc atcgacgcgc
     1561 tcggcccctc gctctatgag cgcttcgggc gtcttgacgt gcttgtcggc aacgctgcca
     1621 tcctcggcgc tctctcgccc ctcccccaca ttccttcagc ccattgggac cgcgtcttcg
     1681 ccgtgaacgt caccgccaat tggcgattga tccgcacgct cgatccgctg ttccgtcgct
     1741 cagacgcggc ccgcgtcatt ttcgtaacct cgggcgtggc gcgatcggcc cgcgcctatt
     1801 gggcgcccta ctcggtctcc aaggctgcgc tcaaggccct ggccaggacc tatgccaacg
     1861 agaccgccga cacgccgatc agggtgaacc tcgtcgatcc cggcgctctt gcgacacgca
     1921 tgcgcgccga agcctatcct ggcgaggatc aagccacctt gccaacgcca gagcaggtga
     1981 cggagatctt cgtgacgctc gccatggcgg aaaatgacgt taccggccag ctctttacgc
     2041 cggaggttta gtgacctctg cgcccgccgc agaatctcca catcttgatg gcagggttgc
     2101 ctcaagcagg cgggtcaacc gccatcgcca agcagggaat gccggccatg cgccatgccg
     2161 gagcagcttt cgcagccttg ttgatcgcgc cctttcccgg cgccgccgcc gagctttgcc
     2221 cggcgctgtc cagcaccacg cccacccctt gcgagagcct gatcgatccg gcaagcgacc
     2281 ttttgcaaaa taggatcaac agccggttga acgtcgtcgg cgcaacaccg gatggcgaat
     2341 cggtgtgggc ggaaagccgc caggcgccct ttcagaccgt cggcggcgcc tcggcactca
     2401 ccaccaccta ttttggcgcc gactatcgcc ttggctcgga cctgctgctt ggagccatgg
     2461 tgcagaggga cgatcggatc gtcaccctgc ctatcgacgg cgaggtgacc gccacggacg
     2521 cctatttggc cggtccctat gcagcctatc ggctgtcctc aaatctcgtc cttggcgcac
     2581 gcgcctcctg gggcaaagcc tccgatggtt ggacgctagc gagcgaggag gcgaacttca
     2641 ccaccgaccg tctgctgacc gaggcgcggc tgagcggcag ttggggcttg ggcgaatggc
     2701 agctgatgcc tgcggccgcc gtgacccatt tgga
//
LOCUS       DATNAE010000172         2755 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_771064, whole genome shotgun sequence.
ACCESSION   DATNAE010000172 DATNAE010000000
VERSION     DATNAE010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_771064"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            299..799
                     /locus_tag="VN518_03780"
                     /pseudo
     CDS             299..799
                     /locus_tag="VN518_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020273.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
     gene            complement(958..1932)
                     /locus_tag="VN518_03785"
     CDS             complement(958..1932)
                     /locus_tag="VN518_03785"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HXE03726.1"
                     /translation="MADASTLDLAPEPPTKLTVKRVVALMNAGAGAFNQKLADDVRSG
                     LAAALAQHGVTAEIRFVEGESLHGAAKRALTQAKRGEIDAVVVGGGDGSIRTVAGVLA
                     ETSVPLGVLPLGTLNHFAKDLGIPLPLEEAAATIAAGTLRIVDLADVNGETFINNSSI
                     GIYPYMVIDRERRRAQHKLAKWMAMVPAFFRMLRHFPRRRLRILAKGFARPYRTPCLI
                     VGNNEYGMELLTFGKRDRLDTGKLWFYVVKPRNPAEFFAMVWRLCFGHLDQARDLDKF
                     ELAAAEISAKASRLPVALDGEVKIMHTPLHYRSRPAALRVLVPHTPRS"
     gene            complement(2008..2187)
                     /locus_tag="VN518_03790"
     CDS             complement(2008..2187)
                     /locus_tag="VN518_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03727.1"
                     /translation="MTTLLYWIVYHLDLGRFGSRALDLAVHGWLRSAKDDEPAPMVRR
                     RSFGELTVLHLEPRI"
     gene            complement(2264..>2755)
                     /locus_tag="VN518_03795"
     CDS             complement(2264..>2755)
                     /locus_tag="VN518_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012336462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HXE03728.1"
                     /translation="PLIANPYAETGVKVVTIADLRWKRRDIKSTSMLAQAMGKQAAKL
                     KGAYEAWMVEDGHINEGTSSSAFILDQDGVIRTQPLGHHILPGVTRRAVLRLAALEGV
                     SLQERPFSVAEALAAREAFMTAASAFVLPVVEIDGVKIGGGRPGPMARKFRALYIEEA
                     RKS"
BASE COUNT          527 a          891 c          886 g          451 t
ORIGIN      
        1 cttgccgagc agatcgccaa acgcttcgtc ggccaattcc tgcacgctcc tgccggtgtc
       61 tttcgccagc atctcgagcg caagccagat ttcgggcgca aactcgatca gcttgcgctt
      121 gatgtaactg gaatgcgggc gagcgaacat agcgataagc ataactgatt cgccccttca
      181 tgagcatgcg ctgggaacct cgaggccctc tcggcgctta acagccgaag cgcttcgggg
      241 ctcgacccga aacgtcccca ctcacgaagc aagaaccgac cgggaaagga cgcaccccat
      301 ggggctgttc agcaaagaca tcaaaacgat ggacgacctt ttcctgcata ccttgcagga
      361 cgtctattac gccgaaaatc agatcgtgaa ggcgctgccg aagatgatcg acaaggccac
      421 caaccgcgat ctcgccgctg gcttgcgcaa ccacctgcgc gagaccgaag gccaggttgt
      481 caggctcgag caggtattcg ccaagcttgg ccagacgccg aaaggcgtcg attgcccggc
      541 gattgacggc ttgatcaagg aggccgacga ggtggccggc gaggtcgacg atacgaaggt
      601 gctcgacgcc gccatcatcg gcagcgcgca ggccgtcgag cattacgaga tctcgcgtta
      661 cgggaccctg atcgcctggg ccgaggagct cggccacggc aatgtggtga gcctgctcag
      721 cgccaatctc aagcaagaga aagccgccga caagacgttg agcggccttg ccgaaggcgg
      781 cgtgaatcgg cgcgcaaccg gacgggccgc acccaaggcg ccagcgcgcc gagcggctgc
      841 ctcgagcgcc acggtgaagc gaaagacctc caccgccaag cggcgccccg ccgccaagaa
      901 gaagacggcg cggcgctagt gtcctgagcc ggggcccgca aacgcgggct ccggcggtca
      961 gcttcgcggc gtgtgtggaa cgaggacgcg gagcgcagca gggcgcgagc gataatgcag
     1021 cggggtgtgc atgatcttca cctcgccgtc caacgccacg ggcagccggc tggccttggc
     1081 cgagatctcc gccgcggcga gctcgaattt atccaggtcg cgcgcctgat ccagatggcc
     1141 gaagcatagc cgccaaacca tggcgaagaa ctccgccggg ttgcgcggct tgaccacata
     1201 gaaccagagc ttgccggtat cgaggcggtc acgcttgccg aaggtcaaaa gctccatgcc
     1261 atattcgttg ttgccgacga tcaggcaggg cgtgcggtag ggccgggcga agcctttggc
     1321 gaggatgcgc aagcgccgcc tcgggaaatg gcgcagcatg cggaagaagg ccggcaccat
     1381 ggccatccat ttcgccagct tgtgctgcgc tcgccgccgc tcgcggtcga tgaccatgta
     1441 gggatagatg ccgatcgaag agttgttgat gaaggtttcg ccgttgacgt cggcgagatc
     1501 gacgatgcgc agcgtcccgg cggcaatcgt cgcggccgcc tcctcgagcg gcaaggggat
     1561 gccgagatcc ttggcgaaat gattgagggt gccgaggggg agcacgccga gcggcacaga
     1621 tgtctcggca agcacgcctg ccacagtgcg aatgctgcca tcgcctccgc cgaccaccac
     1681 ggcatcgatc tcgccgcgct tggcctgggt gagggcccgc ttggcggcgc catgcaggct
     1741 ttcgccttcg acgaatctga tctcggcggt aaccccatgc tgcgccaacg ccgcggcgag
     1801 accgctccgc acatcgtcgg caagtttctg gttgaaggcg ccggccccgg cattcatcag
     1861 cgccacgacg cgcttcaccg tgagcttcgt aggcggctcc ggagcaaggt ctagcgttga
     1921 tgcgtccgcc atgggccctc tttgccgcct ggattcgcag ccaagcaacc tatcagcgcg
     1981 cgaacgcgaa gcgaaagggc aatgccctca aatcctcggc tcgaggtgca gaacggtgag
     2041 ttcgccaaag ctccgccgcc gcaccatcgg ggccggctca tcgtctttgg cgctgcggag
     2101 ccagccatgc acggccaagt cgagcgccct cgacccgaac cggccgaggt cgagatggta
     2161 gacgatccaa taaagcagcg tggtcataga ccgataatgg cgtcgcatgc catccggttg
     2221 catgcgtttt ccggcatttt cacggccgct ccgaggcgcc cggtcacgat ttgcgcgcct
     2281 cctcgatata gagcgcgcgg aacttgcggg ccatcggccc tggccgcccg ccgccaatct
     2341 tgacgccgtc gatctccacg accggcagca cgaaggccga ggccgcggtc atgaacgcct
     2401 cgcgtgcggc caatgcttcg gcgacgctga atgggcgctc ctggaggctc accccttcca
     2461 gcgcggcgag cctgagcacg gcccggcgcg tcacccctgg caagatatgg tggccgagcg
     2521 gctgcgtgcg gatcacgccg tcttgatcga gaataaaggc ggacgatgag gtcccttcgt
     2581 tgatgtggcc gtcctcgacc atccatgcct cgtaagcgcc tttcagcttg gcggcctgct
     2641 tgcccatcgc ctgcgccagc atcgaggtcg atttgatgtc gcggcgcttc cagcgaagat
     2701 cggcaatggt cacgaccttg acccccgttt cggcgtaggg attggcgatc agcgg
//
LOCUS       DATNAE010000173         4780 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2275530, whole genome shotgun sequence.
ACCESSION   DATNAE010000173 DATNAE010000000
VERSION     DATNAE010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4780
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2275530"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VN518_03800"
     CDS             <1..201
                     /locus_tag="VN518_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein B"
                     /protein_id="HXE03729.1"
                     /translation="TGERIGHMNMSGANGTIATFGDLGVKRKIFIHINNSNPALREDS
                     PEREAVEASGWEVAYDGMTIRI"
     gene            205..2127
                     /gene="asnB"
                     /locus_tag="VN518_03805"
     CDS             205..2127
                     /gene="asnB"
                     /locus_tag="VN518_03805"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002712693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HXE03730.1"
                     /translation="MCGIAGFLDLERRSGSQELEALGRAMAARLNHRGPDAHGLWADA
                     EAGVVLGHTRLSIVDLSPAGAQPMVSSCGACVLSYNGEIYNAGDLRPELEARGRRFKG
                     HSDTEVIVEAIAEWGVEPTVERLIGMFAFALWDRRDRTLALVRDRLGIKPLYYGRQNG
                     RLVFASELKAFEVLPNWRPELNRDALAAYLRLAYVPSPHSIYRGIDKLAPGHVASIDA
                     NGTVTISAFWSVEQAAERGKSARFEVSDREATDTLELLLGDAVGRRLVADVPLGAFLS
                     GGIDSSTIAAMMRMRSNAPVRTYSIGFKEEGFDEAPHARAVAAHLGTEHTELYVSPVE
                     AQGVIPELPTIYDEPFADSSQVPTYLLSKLTREHVTVALSGDGGDELFAGYTRHRFAK
                     LASNMPAPVGRALACGLGVAGPELWERVFSVLPAARRPRLAADKMHKAARLFGAGEES
                     GYRSLVSAWDDPDRLVNGGSEPKGPIFDAAIARALPDPLDRMQYLDTITYLPDDILTK
                     VDRASMAVALEVRVPILDHRIVEFSWRLPARFKMRGGKGKWLLRQVLYRHVPRKLLER
                     PKSGFAIPLGTWLRGPLRAWAEELLSEKRLEEGGLLNPAPIRERWAEHLEGTRNWHAS
                     LWTVLMFQAWRSPRGL"
     gene            complement(2135..3121)
                     /locus_tag="VN518_03810"
     CDS             complement(2135..3121)
                     /locus_tag="VN518_03810"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HXE03731.1"
                     /translation="MGSSFSLDPAVPMSEAARRAACSEIDSACAALAVPPDRHKGVHD
                     ARKCLKRLHSLIVLIRPGLPDPVYASLAERLRSIAKGLAPARDAPALLDAINKLFGDA
                     EDDTEATPIPALRKWLQERRQSAARSLESSVASDAIGGLTSVRPAMAGLAVYPDGFQS
                     VAKGLRQSYRGGRKALAEAFASGSAEDFHEWRKALQHHWRHMQLLTPCWPSELSARVE
                     ISRSLSQILGDDHDIAMLQHLISAPTMSFASPDVTASFLKQCRSRQRALRRDAKMLGE
                     RLFAERSQPFAERIEAYWLTATRASAKPTPIARAGNVIAFGEAGAASRRVSG"
     gene            complement(3189..3824)
                     /locus_tag="VN518_03815"
     CDS             complement(3189..3824)
                     /locus_tag="VN518_03815"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007635988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HXE03732.1"
                     /translation="MSARLDPFYPVLPDAAWVARLASEGAKFIQLRVKDASLAAVKTE
                     IAQALKVASAHGCQLVVNDYWREAIGLGADFVHLGQGDLAGADLGVIKATGIKLGIST
                     HSLDELDNALAAGPDYIALGPIYPTKLKAMPWKPQGLARVGEWRARISCPLVAIGGIT
                     LERAPDVLAAGASSAAVVTDIVMNDDPGQRTRDWIAATAPWRHLSTVRPHP"
     gene            complement(3821..4609)
                     /locus_tag="VN518_03820"
     CDS             complement(3821..4609)
                     /locus_tag="VN518_03820"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HXE03733.1"
                     /translation="MFDIYGERFTSRLLLGTAQYPSPATLKEAVEASSAEIVTVSLRR
                     ESARMRAGKGFWELIEQLGVRVLPNTAGCHNAKEAVATAEMARELFDTPWVKLEVIAN
                     DDTLQPELFGLVEAASILVAQGFQVLPYTTEDLSAAEKLAGAGCRVLMPWASPIGSAR
                     GLINRDGLKTLRAYFPDTTLIVDAGIGAPSQAAEAMELGYDAVLLNTAVAKAGDPVKM
                     AEAFARAVEAGRLGFEAGLIEPRDMASPSTPVAGTPFFKIDRVS"
     gene            complement(4609..>4780)
                     /gene="thiS"
                     /locus_tag="VN518_03825"
     CDS             complement(4609..>4780)
                     /gene="thiS"
                     /locus_tag="VN518_03825"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HXE03734.1"
                     /translation="VATDARDLAELCASLGFTEAKVATAVNGCFVASAKRGLTPLAPK
                     DEIEIVAPRQGG"
BASE COUNT          802 a         1535 c         1599 g          844 t
ORIGIN      
        1 accggggagc gcatcggcca tatgaacatg tctggcgcaa acggcacgat tgccaccttc
       61 ggcgatctcg gggtcaagcg taagatcttc atccacatca acaattccaa tccggcgctg
      121 cgcgaggatt cgcccgagcg ggaagcggtt gaggcgtctg gctgggaagt cgcctatgat
      181 gggatgacga taaggatcta gccaatgtgc ggcatcgcgg ggtttttgga ccttgagcgc
      241 cgttctggct ctcaagagct ggaggcgctc gggcgcgcca tggcagcgag gctcaatcat
      301 cgcggccccg acgcgcatgg cctgtgggcc gacgcagagg ccggcgtggt gctcggtcac
      361 acccggcttt ccatcgtcga cctgtcgccg gcaggtgccc agccgatggt gtcgagctgc
      421 ggcgcttgcg tgctgtccta caacggcgag atctacaatg ccggcgatct ccgccccgag
      481 ctcgaggcgc gtgggcggcg cttcaagggg cactccgaca ccgaggtgat cgtcgaggcc
      541 atcgccgaat ggggcgtcga gcccacggtc gagcgcctca tcggcatgtt cgcctttgcg
      601 ctatgggatc ggcgcgaccg gacgctcgcc ctcgtccgcg atcgcctcgg catcaagccg
      661 ctctattacg gacggcagaa tgggcgtctc gttttcgcct ccgagctcaa ggccttcgag
      721 gtgcttccca attggcgccc tgagctcaac cgcgacgcgc tcgccgccta tttgcggctc
      781 gcttatgtgc cgtcgccgca ttcgatctat cgtggcatcg ataagctcgc gccgggccat
      841 gtggcgagca tcgatgccaa cggcaccgtg acgatttccg ccttctggag cgtggagcag
      901 gcggccgagc gcggcaagag cgcgcggttc gaggtgagcg accgggaggc gaccgacacg
      961 ctcgagctgc tgcttggcga cgcggtgggc cggcggctcg tcgccgatgt gccgctcggc
     1021 gcctttctct caggcggcat cgattcctca accatcgcgg ccatgatgcg catgcgcagc
     1081 aatgctccgg tgcgcaccta ttcgatcggc ttcaaggagg agggcttcga cgaagccccc
     1141 catgcgcgcg cagttgccgc tcatctcggc accgagcata ccgagctcta tgtcagtccg
     1201 gtcgaggcgc aaggtgtgat cccggagctg ccgaccatct acgacgagcc attcgccgat
     1261 tcgtcgcaag tgccgaccta tctcctgtcg aagctcaccc gcgagcatgt caccgtggcg
     1321 ctgtccggcg acggaggcga tgagctgttt gccggctata cgcggcatcg ctttgccaag
     1381 cttgcgagca acatgccggc ccccgtgggc cgggctttgg cctgcggcct aggcgtcgcc
     1441 gggccggagc tttgggagcg cgtgttcagc gtgctgcccg cggcgcggcg tccgcggctc
     1501 gccgccgaca agatgcacaa ggccgcgagg ttgttcggcg ccggtgaaga gagcggatat
     1561 cggagcctgg tcagcgcttg ggacgatcct gatcggctcg tcaatggcgg aagcgagccg
     1621 aagggtccga tcttcgatgc ggcgatcgcc cgcgctctcc ccgatccgct cgaccgcatg
     1681 caatatctcg acaccatcac ctatcttccc gatgacattc tcaccaaggt cgaccgcgcc
     1741 agcatggcgg tggcgctcga ggtgcgggtg cccatcctcg accatcgcat cgtggaattc
     1801 tcctggcggc tccccgctcg cttcaagatg cgtgggggca aaggcaaatg gctgttgcgc
     1861 caggtgctct atcgccatgt gccgaggaaa ctcttggagc ggccgaagtc gggcttcgcc
     1921 ataccgctcg ggacgtggct caggggaccg ttgcgggcgt gggccgagga gctcctgtcg
     1981 gagaagcggc ttgaagaagg cggcctgctc aatcccgctc cgatccgcga acgttgggcc
     2041 gaacacctcg aaggcacgcg caattggcat gcttcgctgt ggacggtgct gatgttccag
     2101 gcttggcgaa gcccgcgcgg actctgacgc gctcctagcc cgacacccgg cggcttgcgg
     2161 ctcctgcctc gccgaaggcg atgacattgc ccgcacgcgc gatgggcgtt ggttttgccg
     2221 acgctcgcgt ggcggtgagc caataggcct cgatgcgttc ggcgaaaggc tgagatcgct
     2281 cggcgaacaa tctctcgcca agcatcttgg catcgcgtcg cagtgccctt tgccggctgc
     2341 ggcattgctt caagaaggaa gcggtgacat cggggctcgc aaacgacatg gtcggcgccg
     2401 agatcagatg ttgcagcatg gcgatgtcat ggtcgtcgcc aaggatttgc gacaggctcc
     2461 gcgagatctc gacccgcgcc gaaagctcgg acggccagca cggggtgagc aactgcatat
     2521 ggcgccaatg atgctgcaag gccttgcgcc actcgtggaa atcctcggcg ctgccgctgg
     2581 caaaggcctc ggctaaggcc ttgcggccgc cgcgatagga ttggcggagg cccttggcga
     2641 ccgattggaa cccgtcggga taaaccgcga gcccggccat ggccggcctg acgctggtaa
     2701 gtcccccgat ggcgtcggac gcaacgctgc tttccagact gcgcgcggcc gattgccggc
     2761 gttcttgcaa ccatttgcgc aaggctggga tcggagttgc ttcggtatcg tcctcggcgt
     2821 cgccaaagag cttattgatg gcatcgagca aagcgggggc atcgcgcgcc ggggcaaggc
     2881 cctttgcgat gctgcgcagg cgctcggcga ggcttgcgta gaccgggtct ggcagaccag
     2941 gcctgatcag cacgataaga gagtgtaatc ttttcaggca cttgcgggcg tcgtggacgc
     3001 ctttgtgccg gtcaggggga acggcgaggg ccgcgcaggc tgaatcgatc tcggaacagg
     3061 cggcgcgccg cgccgcctcg ctcatcggca cggccggatc aagactgaaa ctcgaaccca
     3121 tccaaaccct ctccgccaga aggcgcgccc agcgatgttc caggactgcc cgtcctcggt
     3181 caaggggatc aggggtgtgg acggactgtg gataaatgcc tccacggggc ggttgcggcg
     3241 atccagtcgc gcgtgcgctg accgggatcg tcgttcatga cgatgtcggt gacaaccgcg
     3301 gctgatgacg caccggcggc aagcacatcc ggtgcacgct caagggtaat gccgccgatg
     3361 gcgaccagcg gacagctaat cctagcccgc cattcgccga cgcgcgcgag gccttgcggc
     3421 ttccacggca tcgctttgag cttggtcgga tagatgggcc caagcgcgat atagtccgga
     3481 ccggccgcaa gcgcattgtc gagctcatcc aggctgtggg tgctgatgcc gagctttatg
     3541 ccggtagctt tgatgacgcc gaggtcggcg ccagcaaggt ctccttggcc gagatgcacg
     3601 aagtccgcgc cgaggccgat cgcttcgcgc caatagtcgt tgacgacgag ctgacagcca
     3661 tgcgccgaag cgactttgag cgcttgcgcg atctccgtct tcactgcggc gagtgacgca
     3721 tccttgacgc gaagctggat gaactttgcc ccctcgctcg cgagcctcgc gacccaggcg
     3781 gcatcgggaa ggaccggata gaacggatcg agccgggcgc tcaagagacg cgatcgatct
     3841 tgaagaacgg cgtgccggca accggcgtcg agggcgaggc catgtcgcgc ggctcgatca
     3901 gcccagcctc gaagcctaga cggccggcct cgaccgcccg agcgaaggcc tccgccatct
     3961 tcaccggatc gccggccttg gccacggctg tgttcagcag cacggcgtcg tagccgagct
     4021 ccatcgcttc ggctgcttgc gacggcgcgc cgatgccggc gtcgacgatc aacgtcgtgt
     4081 cggggaagta ggcgcgtagc gtcttcagcc cgtcgcgatt gatcagcccg cgcgccgagc
     4141 cgatcggcga ggcccagggc atcagcacgc ggcacccggc cccggcaagc ttctcggcag
     4201 cgctcaaatc ttcggtggtg tagggcagga cctggaagcc ttgcgccacg agaatagaag
     4261 ctgcctcgac gagaccgaac agctctggct gcagcgtgtc gtcgttagcg atgacctcga
     4321 gtttgaccca aggcgtgtcg aacagctctc gcgccatctc cgccgtggcg acagcttcct
     4381 tggcgttgtg gcagccggcg gtattgggca gcacgcgcac gccgagctgt tcgatcagct
     4441 cccagaagcc tttgcctgcg cgcatccgcg ccgactcgcg gcggagcgaa accgtgacga
     4501 tctccgctga cgacgcttcg accgcttcct tcagcgtcgc gggggaggga tattgcgccg
     4561 ttccgagcag cagcctggac gtaaagcgct cgccatagat atcgaacatc agccgccttg
     4621 cctcggcgcc acgatctcga tctcgtcctt cggcgcgagc ggcgtcagcc cgcgcttggc
     4681 ggaagcgacg aagcacccgt tgactgcggt cgcgaccttg gcctcggtga agccgaggct
     4741 ggcgcaaagc tcggcaaggt cgcgcgcatc ggtggcgacg
//
LOCUS       DATNAE010000174         2195 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_4385204, whole genome shotgun sequence.
ACCESSION   DATNAE010000174 DATNAE010000000
VERSION     DATNAE010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4385204"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="VN518_03830"
     CDS             complement(<1..638)
                     /locus_tag="VN518_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03735.1"
                     /translation="MPIRLERRRAVRWTANTSLLRLSFGVGEGALALVAVLLPTLFYH
                     VVFGHAALTDIRWGLYGAYAGIAALVYGGFSIEAAGKLLENGRQQQAVIADSALAWTG
                     AFAVALLAAFLADLTDQLSRVSMLAAYLVGVPLLIGGRAAAHTIASSYIRAGRLQYRK
                     AGVIGNRGDTVRFLLNGSLWRAGYRLAGTLYLEEARRPDGRLDEAAIADIAQR"
     gene            complement(879..1412)
                     /locus_tag="VN518_03835"
     CDS             complement(879..1412)
                     /locus_tag="VN518_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXE03736.1"
                     /translation="MAAERLLLIVEDDEGFARTLKRSFERRGYTVVLAASVEEVEALL
                     ETQTPGFAVVDLKLAGASGLVCVERLHAHDPEMMIVVLTGFASISTAVEAIKLGACHY
                     LAKPANTDDIEQAFGRGAGDASVALTQRPTSFKTLEWERIHEMLVETDFNISEAARRL
                     GMHRRTLARKLEKRPVK"
     gene            complement(1402..>2195)
                     /locus_tag="VN518_03840"
     CDS             complement(1402..>2195)
                     /locus_tag="VN518_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HXE03737.1"
                     /translation="LDAALLIAFVTRIGRNLRIRDAHLAALRQHAIEEDHIVRMGLLA
                     SGAAHELGTPLASMSVILGDWRRMPQLEADDDLRQDIEDMQMAVARCKAILTGVLLTA
                     GEARGEAPAVTTMAHFLDELVADWCKLRSVTTLHYVNAFEGDLAIVSDTALKQVVFNV
                     LDNAYEVSPSWLSFTAERRGEMLELKVSDAGPGFSAEMLKRFGRPYQSTKGRPGGGLG
                     LFLVVNVVRKLGGTVTPGSRRTGGAVVTIELPLETLMIGQEDHGG"
BASE COUNT          408 a          720 c          706 g          361 t
ORIGIN      
        1 cgttgcgcga tatcggcgat agccgcctcg tcgagcctgc cgtcagggcg gcgtgcctct
       61 tcgagataga gcgtgccggc aagccggtag ccggcgcgcc aaagcgaacc gttgaggagg
      121 aagcggacgg tgtccccgcg attgccgatg acacctgcct tgcgatactg cagccgccct
      181 gcccggatat aggaactggc gatcgtatgg gcggcggcgc ggccgccgat cagcagcggc
      241 acaccgacga gataagcggc gagcatcgaa acgcgcgaca gctggtcggt gaggtcggca
      301 aggaatgctg ccaacagcgc gaccgcgaag gcgcccgtcc aagcgagcgc gctgtcggcg
      361 atcacggcct gctgctgccg accgttctcg agcagcttgc ctgccgcctc gatcgagaag
      421 ccgccataga cgagagcggc gatgccagcg taagccccat agagacccca gcgaatatcg
      481 gtaagggcgg cgtgtccgaa gacgacgtga tagaagagtg tcggaagtag aacggcaacg
      541 agcgccagcg ccccttcgcc gaccccgaac gagagacgaa gcaggctagt gttggccgtc
      601 cagcggacgg cgcgccggcg ctccagccgt ataggcaatc cgagctgttc ctgtgtcacg
      661 ccgcctgccc tcaagtacca accgttcaag tcgccaaacg agatagcacg cggcgggcgc
      721 gccccccaat cacgggttaa ggaacggtta acgcgccaga tttgcttcgc tttttacgcg
      781 atcgagaaat agctctcccg caggcgcggc aggaattcgg ggatccccgc gggaagcgca
      841 atcgactgcc tcgcttcagc ctccccggcc tgcatcggct acttgaccgg acgcttttcg
      901 agttttcggg cgagggttcg tcggtgcatg ccaaggcggc gggccgcctc cgagatgttg
      961 aagtcggtct cgaccagcat ctcatggatt cgctcccatt cgagcgtctt gaaggatgtc
     1021 ggccgctggg tcagcgctac gctggcatcg cctgcgccgc ggccgaaagc ctgctcgatg
     1081 tcgtcggtgt tggccggctt ggcgagataa tgacaggcgc cgagcttgat cgcctcgacc
     1141 gccgtcgaga tgctggcgaa accggtcagc accacgatca tcatttcggg gtcgtgagcg
     1201 tgcaatcgtt cgacacagac gaggcccgat gcgcccgcca gcttgaggtc gaccaccgcg
     1261 aagccgggcg tctgtgtttc gagcagcgct tcgacctcct cgacgctcgc tgccagcacg
     1321 acggtatagc cgcgccgctc gaaggagcgc ttcaaagtcc tcgcaaaacc ttcgtcgtcc
     1381 tcgacgatca gcaaaagccg ctcagccgcc atggtcttcc tgtccgatca tcagcgtctc
     1441 gaggggcagc tcgatcgtca ccaccgcgcc gcctgtgcgc cgactgccgg gcgtcacggt
     1501 gccgccgagc ttgcgcacca cattgaccac aagaaacagg ccaaggccgc cgcccggcct
     1561 tcccttggtc gattgatagg ggcgcccgaa gcgcttgagc atctccgcgc tgaaaccggg
     1621 gcccgcgtcg ctgaccttga gctccagcat ttcgccacgc cgctcggcgg taaagctcag
     1681 ccaactcggc gacacctcgt aagcattgtc gagcacattg aacacgacct gcttgagcgc
     1741 ggtgtcggag acgatcgcca ggtcgccctc gaatgcattc acgtagtgta acgtagtgac
     1801 cgaacgcagc ttgcaccagt cggcgacgag ctcatcgagg aaatgcgcca tggtggtgac
     1861 ggccggcgct tcaccgcgcg cctctccggc ggtcaacagc acgccggtca ggatggcctt
     1921 gcaccgcgcc acggccatct gcatgtcctc gatgtcctgg cggagatcgt cgtcggcttc
     1981 cagctgcggc atgcggcgcc agtcgccaag gatgaccgac atcgaggcga gcggggtgcc
     2041 gagctcatgc gccgcgccgg aagccagcag gcccatgcgg acgatatggt cttcctcgat
     2101 ggcatgctgg cgcagggccg ccagatgtgc atcacgaatc ctgaggttgc gcccgatgcg
     2161 ggtgacgaag gcgatgagca gtgccgcatc cagcg
//
LOCUS       DATNAE010000175         2839 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_776371, whole genome shotgun sequence.
ACCESSION   DATNAE010000175 DATNAE010000000
VERSION     DATNAE010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776371"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..1213)
                     /locus_tag="VN518_03845"
     CDS             complement(5..1213)
                     /locus_tag="VN518_03845"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HXE03738.1"
                     /translation="MDTFDPTNLPDTPDIPASIVASGLSDAPLTTLVEAAMQRLVASG
                     VPIDRMNVGFRILHPLFDGMSITWTRETGAEVFYAALVDKEGSEFRVSPFYVMLSDGS
                     REWRLRLDRDDIERFPLLMQLREKGFTDYLALIVAFGGAELHPQSLDGLATSWSTRSP
                     QGFSPSDIAAMRGVLTPLALVFRLAIKDQITKSALNTFHGPLVGGRILSGTIKRGDGE
                     RLAAALWYSDLRNSTGLADQLPVDAFLDLLNAYFDCAAGAVMEQGGQVLDIIGDAILA
                     FFPAGDNGGAEACALALEAAAGARERLAELKREGSGKARAIEFGIGVHFGDVVFGNAG
                     TPERLKFGVLGRAVNDVAHTADMSKSLKRPVVVSDEVARRANVTLADLGLHELRGIPQ
                     ARRLWAMEVG"
     gene            1342..1746
                     /locus_tag="VN518_03850"
     CDS             1342..1746
                     /locus_tag="VN518_03850"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXE03739.1"
                     /translation="MKRVVIELGIVVAVLLGGFALIWFVPGVQDRIVQSAMTRQIAKN
                     ARAPYLSEDALHILLCGTGSPMPDMTRANACAAVIANGRVVVIDAGPGAWSRLAAANI
                     PGAKIDTVLLTHLHSDHIGDLGEVATQPRPRC"
     gene            1758..1898
                     /locus_tag="VN518_03855"
     CDS             1758..1898
                     /locus_tag="VN518_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03740.1"
                     /translation="MARPGPDEAVTVHDKDGLKISAFLVNHDPVEPAYGYRIEYAGGP
                     RW"
     gene            1892..2287
                     /locus_tag="VN518_03860"
     CDS             1892..2287
                     /locus_tag="VN518_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03741.1"
                     /translation="MVSGDTARVENVVRFSKGADVLVHEALDRDMVEMLAKALDASGN
                     SRAGTMAPQVVAYHTSPVDAAAIAKEAGVPLLVFTHEVPPLRNGLMRRMLMRGVTDGR
                     GDGKTILGKDGLLISLPKGTKDIQTKNLW"
     gene            2493..2717
                     /locus_tag="VN518_03865"
     CDS             2493..2717
                     /locus_tag="VN518_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03742.1"
                     /translation="MKKYLIASLILAATTVPSFAAAQHFVVEDTEKYCSVIDAAPSKD
                     LKVLGQRQGYGSRAQAETALKGLNCKALPG"
BASE COUNT          502 a          887 c          977 g          473 t
ORIGIN      
        1 cgcgttaccc gacctccatc gcccataacc tccgggcttg cggaatgccg cgaagctcgt
       61 gcaggccgag atcggcgagc gtgacgttcg cgcgccgtgc aacctcgtca ctcaccacca
      121 ccgggcgctt cagcgacttc gacatgtcgg cggtgtgcgc cacgtcattc accgcgcggc
      181 cgagcacgcc gaatttgagc cgctccggcg tgccggcatt gccgaacacc acatcgccga
      241 aatgaacgcc gatgccgaac tcgatcgcgc gcgccttgcc gcttccctcg cgcttcagct
      301 cggcgagccg ctcgcgcgcg ccggcagcgg cctctagcgc gagcgcgcag gcctcagccc
      361 cgccattgtc accggcgggg aagaaggcga ggatggcatc gccgatgatg tcgagcacct
      421 ggccgccctg ctccatcacc gcgcccgcgg cacaatcgaa ataggcgttg agcaggtcga
      481 gaaaggcatc gaccgggagc tgatcggcaa ggcccgtcga gttgcgcaga tcgctgtacc
      541 acagcgccgc ggcgagccgc tcgccgtcgc cgcgcttgat ggtgccggag aggatgcggc
      601 cgccgaccag aggcccgtgg aacgtgttga gcgctgactt ggtgatctgg tccttgatgg
      661 cgagcctgaa gacgagcgcg agcggggtga gcacgccgcg catggcggcg atgtcgctcg
      721 gactgaagcc ttgcgggctc cgcgtcgacc acgaggtggc gagcccatcg agcgattgcg
      781 ggtggagctc ggcgccgccg aaggcgacga tcagcgcaag ataatcggtg aagcccttct
      841 cgcggagctg catgagcagc ggaaagcgct cgatgtcgtc gcgatcgagg cggaggcgcc
      901 attcgcggct gccgtccgac agcatgacgt agaatgggct cacgcggaac tccgagccct
      961 ccttgtcgac gagcgcggcg tagaacacct cggcgccggt ctcgcgcgtc caggtgatgc
     1021 tcatgccatc gaacagcgga tgcaggatgc ggaagccgac attcatacgg tcgatgggga
     1081 cgcctgaggc gacgaggcgc tgcatggcgg cctcgaccag ggtggtgagc ggcgcgtcgg
     1141 agaggccgga ggcgacgatc gaggccggga tgtcaggcgt gtctggcaga ttggtggggt
     1201 cgaacgtgtc catgctggcg caacttatcg gggaattgcg tggcaatttt gagggcgcta
     1261 ccgcgcttcc acacccgtgt gttcgcgcac cttgagcgca attgagatac agtccgcgcg
     1321 gaaacttgcg gggagacgcc tatgaagcgg gttgtgatcg agcttggcat cgtggtcgcg
     1381 gtgctgcttg gaggatttgc gctgatctgg ttcgtgcctg gcgtgcagga ccgcatcgtg
     1441 cagagcgcca tgacgcggca aatcgccaag aacgcccgcg cgccgtacct cagcgaagac
     1501 gctttgcaca ttctcctgtg tggcaccgga tcgcccatgc ccgacatgac gcgggccaat
     1561 gcctgcgccg cggtgatcgc gaacggccgc gtggtggtga tcgatgccgg gcctggcgcc
     1621 tggtcgcggc tcgccgccgc caacatcccc ggcgccaaga tcgacaccgt gctgctgacg
     1681 catctccatt cagaccatat cggcgatctc ggcgaggtgg cgacgcaacc gaggccgcgc
     1741 tgctgatcgg tcatgacgtg gcgcggcccg gcccggacga ggcggtcacc gtccatgaca
     1801 aagacgggct caagatctcg gccttcctcg tcaatcacga tccggtcgag cctgcttacg
     1861 gctaccgcat cgagtatgcg ggcgggccgc ggtggtgagc ggcgacacgg cgcgggtaga
     1921 gaacgtggtg cgcttctcga aaggcgccga tgtgctggtg catgaggcgc tcgaccgcga
     1981 catggtcgag atgctggcaa aagcgctcga cgcctccggc aacagccgcg ccggcaccat
     2041 ggcgccgcag gtcgtcgcct atcacacctc gccggttgac gctgccgcga tcgccaagga
     2101 agcgggcgtg ccgcttctcg tcttcaccca tgaggtgccg ccgctcagga acggactgat
     2161 gcgccgcatg ttgatgcgcg gcgttactga tgggcgcggc gacggcaaga ccattctcgg
     2221 caaggatggg ctcttgatct cgctgccgaa gggcacgaag gacatccaga cgaagaattt
     2281 gtggtgactc tttttgtgcg ccaccgcttc gctgcttgag cgcttctttg atgcgctacc
     2341 gcttcgccgc tttagcgcag tgcggcgggg tgacgcttgc gcagattggc cgcggcgaag
     2401 agcgagcgcg agagcctccg gctgactaca tctctcgccg ggaaggatgg catcatcctc
     2461 gtggggaggc ggcttcagaa ggaggcacct ctatgaagaa atatctgatc gcaagcctga
     2521 tcctcgccgc aacgactgtg ccgtcgtttg ccgccgcgca gcatttcgtg gtcgaggaca
     2581 ccgaaaaata ttgcagcgtc atcgacgcgg cgccgagcaa ggacctgaag gtgcttgggc
     2641 agaggcaggg ctacggctct cgtgcccagg cagagacggc cttgaagggc ctgaactgca
     2701 aggcgctgcc gggctaaagc cgcaattgag tgccacgcca ccggtcaggc aaagattgcg
     2761 tgaagggtga cgcttggaga gtgcgcgagc catatgcacc acggccgctt cgatcgtggt
     2821 ctgcaacgcg aaggcaagg
//
LOCUS       DATNAE010000176        15550 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3178228, whole genome shotgun sequence.
ACCESSION   DATNAE010000176 DATNAE010000000
VERSION     DATNAE010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 15550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15550
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3178228"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3147)
                     /locus_tag="VN518_03870"
     CDS             complement(<1..3147)
                     /locus_tag="VN518_03870"
                     /inference="COORDINATES: protein motif:HMM:NF019323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HXE03743.1"
                     /translation="MPAWLRAGLFAALFVFAAAPLTPVQSAEKTFQDAALDDAAIKLE
                     ADLKDEAGTVEKPVITLRKDADAALRKGDVESAADIYVQVVSVAPNDAAAWRRLADLW
                     LKIPATDEDDGTTRYERATTASYIAYQRAGTASEEADSLVMLATAYGKRSEWRPALNL
                     LALALKLDETPELKTTYDLLREKYGFRVSNFSVDSDAASPRACFQFSESLPKRTDFSP
                     YVAVAGQDKPALSVDDQQICVEGLKHGESYSVSLREGLPSTVGEDLLKTADFSIYVRD
                     RSPFVRLAGKAYVLPKTGQQGIPLVSVNTDTIKVTIYQIGDRNLIDSVLGGDFERNLY
                     GYTLNDIAEQKGEQVWTGEMKVEKELNQEITTNFPVNEAVPALQPGVYVLAAVPANAL
                     TEEYAERATQWFIVSDLGLTAYSGSDGVHAYVNSLATTALVSGVELRLIARNNEVLST
                     KTTGADGRVAFEPGLSRGEGGLAPALLVASLASGDYAFLSLKSSPFDLTDRGVAGRDA
                     PTGLDAYVFTERGVYRTGETVHITALLRNAVSVAVPDVPLTMVVTRSDGVEYRRSVVP
                     DQGIGGRSLDVPIISSAPTGTWRIAAFTDPKQPAIGEATFLVEDYVPDRLEFDLTTTA
                     TSISPASPAKIDVDGRFLYGAPASGLTLDGEINIAKVTELPGFAGYVFGLDDDSDAEQ
                     DDESDTIPLADMPETDSAGKASFTATLDKVPASMRPQQAEIVVRMAEAGGRAVERKLT
                     LPIAPSAAMIGVKPLFTGKALGDSDTASFDVVMVAPDGKAMAKTGLHWQLLRVDSKYQ
                     WYRNEGYWQYEPVKVTRRIADGTIDVSTDKPGRIAVPVTWGRYRLELMTADPPGPETT
                     FGFDSGWYAEASADTPDLLEIALDKPEYRPGDSMTVAVTARTAGDVTLAVIGDKLLST
                     TTAHVEPGTAKLPLTVGADWGTGAYVLATLRRPLDVDAKRMPGRAIGVAWFAIDKAAH
                     TLGVSMDLPDRVRPETTLRVPIKLAGLTPGDEARVVVSAVDVGILNLTNYKAPAPEDY
                     FLGQRKLSAEVRDLYGQLI"
     gene            complement(3235..4386)
                     /locus_tag="VN518_03875"
     CDS             complement(3235..4386)
                     /locus_tag="VN518_03875"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HXE03744.1"
                     /translation="MKRRAPFLSLLFSAILSLASSAAQAAEPGAIENDLVITVQGEER
                     HVSLDEALKLLDIPSASVALIDEGRIAFASAYGKEATPDTVYQAASLSKFVAAIGAMR
                     LVEDGTLDLDQGVNDKLTSWRVPGNAFDATHKVTLRGLLSMTGGIGVPGFLGYEAGAP
                     LPTLGQILDGAPPANSPPVTEIAVPGSAYHYSGGGYEIAEALMQDATGKPFPQLMQDL
                     VLGPMGMTASSFEQPPSAAFAAKAASGHFSDGTELPGRWHAFPEHAAAGLWSTPTDLA
                     KLLVELADVWQGLSSIFLRRQTLEEMLTPQNGGPYGLGAAIAGEGASLVLMKRAQNVG
                     YQGYLILYPASGQGLVLMTNSDNGSKLAEALIKRAASAYGWPELPPLAD"
     gene            complement(4416..5336)
                     /locus_tag="VN518_03880"
     CDS             complement(4416..5336)
                     /locus_tag="VN518_03880"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HXE03745.1"
                     /translation="MHLKEVDANLLVILDALLVDASVTRAAERLGRSASAISHALAKL
                     REIFADELFVRAGQRLVPTAKAQELAPTVHVILAGMESLLRPGKPFDPRDAERDFAMT
                     ASEVGDLILVQPLRRRLEALAPSVRVGWMPGQGEETIEALRQGRCHFAIDIEAMELPA
                     PDIRVLKLYDDRLACVARSGHPMSERKPDARSFAASDHIAVSALPTIELLDRELAKQR
                     LTCFISAQVPSVLAGLLLTLNSDALLTLPASLASILEKQFALARVKLPIDPVSFPVRL
                     LWHSSYDRDECHNWVRDEIATLAREARRAA"
     gene            5597..6925
                     /locus_tag="VN518_03885"
     CDS             5597..6925
                     /locus_tag="VN518_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HXE03746.1"
                     /translation="MMRIASLARPVALVALGICLGLPPAAADEPSGKDVLKTYADIAA
                     AGYADSLATTRTLQLAVNQLLANPTDQNMLAARAAWVAARIPYMQTEAFRFGNKIIDD
                     WEGKVNSWPLDEGLIDYVSGSYGTDSPENELYAADVIANTSLTIGGKKLNTSKITKEL
                     LADKLQEAGGVEANVATGYHAVEFLLWGQDLNGTGPGNGNRPASDYNVKKCTGGHCDR
                     RAQYLHAATDLLVDDLAWMAAQWAPGGEARKTIQDGSAEAGLTAILTGLGSLSYGELA
                     GERMKLGLMIHDPEEEHDCFSDNTFASHYFDALGIRNVYVGRYRRVDGSVVQGPSVSD
                     LIKAKSPEVDNAVRAKIELTMDAMGELYRRGVMVESYDQMIGEGNEVGYATVQKVVDS
                     LLNQTKEIERAVAALDLKSIKFEGSDSLDDPNKVKEENKSAGVAAPANAQ"
     gene            7086..8456
                     /locus_tag="VN518_03890"
     CDS             7086..8456
                     /locus_tag="VN518_03890"
                     /inference="COORDINATES: protein motif:HMM:NF016830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HXE03747.1"
                     /translation="MLLAGRSLRAIALGGMLAAGLATLPSAARADCDVPDNGIPEESL
                     LSKLGPAWVSLGGLRPALAKGGIGVSATYYGEAFANSGGFKQGGKYDGVLGVAIDADL
                     HELGFWKGLCFHTNGFQIHGQSITASNIGSLMPVSSLEATPNTRLFELWLEQHMFGDK
                     LAVKVGQLAADTEFILSEGGGFFLNGTWGWPSITAADLPSGGPAYPLATPGVRVAVTP
                     SDKFQLLVGVYNGDPAPPCGSDDPQVCNSNGLDFELDDPPLLMVEGAYKYNQERLAGT
                     VKLGGWNHFGDFEHQRFSAGGNLIAVTGDPGKPLDNDWGFYGIIDQLIWRLPGSEEAK
                     GAALFGRVIGAPSDRNLIDFYADGGITFSGMIRRRPDDGFAIGFAYTGISDQVHGFDI
                     DSGAPVARNYEALLEICYTMQLATGWTLQPDFQYIWQPGGNVPDEDGRAVENAAVFGA
                     RSTMSF"
     gene            8482..10068
                     /locus_tag="VN518_03895"
     CDS             8482..10068
                     /locus_tag="VN518_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007068412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HXE03748.1"
                     /translation="MHRNRSKSRCRVVPALCGASPKPVSPRKRSLAALIALGASLPLV
                     LGAWESQGAGADASVPGLTAPATNFSKTEEGEDKPGGGATSKASVTNANAFSASSGNM
                     DFKRELDFKIGNAIFRKNWVSAPASTDASDGLGPLFNSRACQNCHLKDGRGHPPFTKG
                     IPDDSHSMLMRLSVPAKTDEEKAKLASHRLNSIPEPTYGGQLQDLSIQGHKAEGQIKI
                     DYKEKPVKLAGGETVHLRVPQYSIVDLGYGPLAPGTMMSPRVAPPMIGLGLLEAVPEE
                     QILANADPDDANQDGISGKPSQVWSRENNKVMLGRFGWKAGVPSINQQAAEAANGDIG
                     LSTTMIRAASGDCTGSEKDCLDAPNGNSPQYQDVELGDDLFKLVAFYSHNLAVPPRRD
                     PADPKVLKGKELFYAIGCASCHKPKFVTGEAPGEPHLSHQLIWPYTDLLLHDMGEGLA
                     DHRPDGEASGSEWRTAPLWGIGLTETVNGHTLFLHDGRARSLTEAILWHGGEGQASRN
                     KFAKLSKEDRERLIAFVSSL"
     gene            10239..11291
                     /locus_tag="VN518_03900"
     CDS             10239..11291
                     /locus_tag="VN518_03900"
                     /inference="COORDINATES: protein motif:HMM:NF020932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HXE03749.1"
                     /translation="MLLLSGTMALHAEADHAAIAKQSLEQYIRPGYAHLAESTAALNQ
                     SVAAVCRTPSEAALKDAKEAFAATVQAWSLVEPIRFGPVAEQHRYERIFYWPDPKGLG
                     ARQVREALDKEDATVTEAASLSAKSVALQGLPALEYLHYGDDAGTLAKGGGKATFRCR
                     FAETIAANLGGMAKDIAAGWQDSAPYAKAYLEPGHDNAAYHIQKEVTLELFKTFTTGI
                     EMVRDQKMAKALGAKPDQARPQLAPFWRSDLSFSNMADNLQSVRELFAKGGFAEVVHD
                     DSPGVEDSIVFDLNHAIEVLRGLDKPITQAVHNEDLRAKLEALRVSLKSAATTAGDII
                     SRSAGLTFGFNAMDGD"
     gene            11294..12397
                     /locus_tag="VN518_03905"
     CDS             11294..12397
                     /locus_tag="VN518_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007008471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1513 domain-containing protein"
                     /protein_id="HXE03750.1"
                     /translation="MIIDRRELLIGAASSAASLALPWHAKAASSEELFAAARRDDRGT
                     YSAALFSLDGGDIRAVELPERGHDIALRPGSGEWVAFARRPGRFGVAVPTDGRQPIWF
                     HAKPDRHFFGHGVYSADGKLLYTTENDYQNAQGIIGVRDATAFYRQIGEFPARGMEPH
                     DVALLADGRTMVIANGGIKTHPDSGADELNLPDMKPSLVYVDLATGDLVEEQILAPEL
                     HQLSMRHLALAASDTVVFGCQYRGPEDDAPPLLGFHRRGERPVIVPAPTGTQTALKNY
                     IGSVSADAGGGIVAASAPKGGLITYWDVGARRFLGSCELNDGCGIAPTRHKASFLLTS
                     GQGWLVETNVADAPEEDRRSSPYEWDNHAILVR"
     gene            12613..14850
                     /locus_tag="VN518_03910"
     CDS             12613..14850
                     /locus_tag="VN518_03910"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HXE03751.1"
                     /translation="MRMKLLLKIGAGVVALLVLALVAFNLLISATAVRDRVAARVKEQ
                     TGRELTVNGSSSVLLMPSPHIVLTDVTIVDPENRAGADLKVARLALDVSFGQLFSREV
                     DAKRVVMERPVFTVRPKTQAGTQQQGDAGSTPEKHAAAELRVIAAGAAFAATRDISLD
                     DVEIEDGTVRIIYDENGAERRIEKINANLSLPHLVDPLTAKGDFDWKGIRVGFDLKLT
                     SPADLETRSARLDLALDTDAIDANFGGNVSTKPAFSAEGDLTAKSQSVPSLLAWLRKQ
                     PPTETAVGSGELSSHVVWQPGEISLTQVRFALSHATGQGQAVVTLKSPRPHLRAALAL
                     ETLDLNPLLASNGAQAAPQAPSGPAPPATDSAAPAEPSPSTGSGEKGDNERAATESGE
                     AQGESTAPSPEAGSPKADRTAAPNVAPAAFDADVNLNVHETKILQMTIGPSSASLSLR
                     DGVLDATLADMQLYDGQGSGKFTLDASKPVPTFAGNLALNGVSAQPLLNGAAGFNLLA
                     GKTNVALQLSGTGGSEDEVKHSLAGQGSIAISDGSIEGINLTELIQGVGAGQMPNLEQ
                     GPGAKTAFSSFGVSFNIASGVAETHDLEMTSPLLKVTGRGTVDVVTSSLDFLTQPEIV
                     AGPEGKGGANSLAGLSIPVRIEGPFDRPTFKPEIKSMFSNPEQASKTINQIGDVLQKK
                     LKGKPVGEAIGRILGGVRIGNERSNGGGGQNAKPPANQEEPDEEGPPEETDQGRDPDL
                     DNILR"
     gene            15045..>15550
                     /locus_tag="VN518_03915"
     CDS             15045..>15550
                     /locus_tag="VN518_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HXE03752.1"
                     /translation="MARQPYLADVDPHEGVARAHAMPVQGIDVSNWQGDIDWNKVRAA
                     GTQFAFIKATEGGDYLDPKFAQNWQAAKSVGIPRGAYHMVYWCRAAHEQALWFILNVP
                     NDPDALPPVLDLEWNGHSRGCPKRIPVKLALEKIKIMLEVMEAHTGKRPIIYTDVAFH
                     REVLEGELP"
BASE COUNT         2801 a         5018 c         5081 g         2650 t
ORIGIN      
        1 gatcaattgg ccgtaaaggt cgcgaacctc ggccgaaagc ttgcgctggc cgaggaaata
       61 atcctcgggc gcgggcgcct tgtagttggt gaggttgagg atgccgacat ccaccgccga
      121 caccacgacg cgggcctcat cacctggggt gagcccggca agcttgatcg gcacgcgaag
      181 cgtcgtctca ggcctgacgc ggtccggcaa atccatggag acgccgagcg tatgcgccgc
      241 cttgtcgatg gcgaaccagg caacgccgat ggcgcggcct ggcatgcgtt tggcgtcaac
      301 gtcgagcggg cgccgcaaag tggccagcac ataggcgccg gtgccccaat cggcaccaac
      361 ggtaagcggc aacttggcgg tgccgggctc gacatgcgcc gtggtggtgg agaggagctt
      421 gtcgccgatc acggcgaggg tcacgtcgcc cgcggtgcgc gcggtcaccg cgacggtcat
      481 gctgtcgcct gggcgatatt caggcttgtc gagggcgatc tcgaggagat caggcgtgtc
      541 ggcgctcgct tcagcgtacc agccagaatc gaagccgaag gtggtctcag gccctggcgg
      601 gtcggccgtc atcagctcga ggcggtagcg gccccaggtc accggcacgg cgatgcggcc
      661 cggcttgtcc gttgacacgt cgatggtgcc gtccgcgatg cgccgcgtca ccttgaccgg
      721 ctcgtactgc caatagcctt cgttgcgata ccactgatat tttgaatcga cgcgcagcag
      781 ctgccagtga agcccggtct tggccatggc cttgccgtcg ggcgcgacca tgaccacgtc
      841 gaagcttgcc gtgtctgaat cgccgagcgc cttgccggtg aacagcggct tcaccccgat
      901 catggctgct gacggcgcga tgggcagcgt gagcttgcgc tcgaccgcgc ggccgcctgc
      961 ttccgccatg cgcaccacga tctcggcttg ctggggccgc atcgacgcgg gcaccttgtc
     1021 gagcgttgcg gtgaagctcg ctttgccggc gctgtcggtc tcgggcatat cggcaagcgg
     1081 aatggtgtcg gactcatcgt cctgttctgc gtcgctgtcg tcgtcgaggc cgaacacgta
     1141 gccggcaaag cccggcagct cggtcacctt ggcgatgttg atctcgccgt cgagcgtgag
     1201 gcctgaggcg ggcgcgccgt acaggaagcg cccgtccacg tcgatcttgg cgggcgaggc
     1261 cggcgagatg ctggtggcgg tcgtcgtcag gtcgaattcg agccgatccg gcacatagtc
     1321 ctcgaccagg aaggtcgcct cgccgatcgc gggctgcttg ggatcggtga aggcggcgat
     1381 ccgccaagtc ccggtcggcg ccgaggagat gatcggcacg tcgagcgagc ggccgccgat
     1441 gccctggtcc ggcaccacgc tgcggcgata ttccacgccg tctgagcggg tgaccaccat
     1501 ggtgagcggc acgtcgggga cggcgacgct caccgcattg cgcaacagcg ccgtgatatg
     1561 cacggtctcg ccggtgcgat agacgccgcg ctcggtgaac acataggcat cgaggcccgt
     1621 cggagcgtcg cgaccggcga cgccgcggtc ggtgaggtcg aagggcgagg acttgaggct
     1681 caggaaagcg tagtcgccgg aggcgagcga ggcgacgagg agggcaggcg ccaatccgcc
     1741 ttcgccgcgc gacagaccag gctcgaaggc gacgcggccg tcggcgccgg tggtcttggt
     1801 ggagagcacc tcgttgttgc gggcgatcag cctgagctca acgcctgaga ccagcgcggt
     1861 ggtggcaaga gaattgacgt aggcatgcac gccgtcagag ccggaatagg cggtgaggcc
     1921 gagatccgag acgatgaacc attgggtggc gcgctcggca tattcctcgg tgagcgcatt
     1981 ggccggcacg gcggcgagca cgtagacgcc gggttgaagc gcaggtaccg cctcgttgac
     2041 cggaaagttg gtggtgatct cctggttcag ctccttctcg accttcatct cgccggtcca
     2101 cacctgctcg cccttctgct cggcgatgtc gttcagcgtg tagccgtaga gattgcgttc
     2161 gaagtcgccg ccgagcactg agtcgatcag gttgcggtcg ccgatctgat agatggtcac
     2221 cttgatcgtg tcggtgttga cgctgaccag cggaatgccc tgctggccgg tcttgggcag
     2281 cacgtaagcc ttgccggcga gccgcacgaa ggggctgcgg tcgcgcacgt agatgctgaa
     2341 gtcggcggtc ttgagcaggt cctcgccgac cgtggagggc aggccctcgc gcaagctgac
     2401 cgaatagctc tcgccgtgct tcagaccttc gacgcagatc tgctggtcgt cgacggaaag
     2461 cgcaggcttg tcttggcctg ccacggcaac gtatggcgag aagtcggtgc gcttcggcag
     2521 gctttcgctg aactggaagc aggcgcgcgg cgaagcggca tccgaatcga cgctgaagtt
     2581 ggaaacgcgg aagccgtatt tctcgcgcaa caggtcgtag gtggttttca gctccggcgt
     2641 ttcgtcaagc ttgagcgcaa gagcaagcaa gttcagcgcc ggccgccact cactgcgctt
     2701 gccgtaggcg gtggcaagca tgacgaggga atcagcttct tccgaagcgg tgcccgcgcg
     2761 ctgataggcg atgtaggacg cagtcgtcgc ccgctcatag cgggtggtgc cgtcatcctc
     2821 atcggtggcc gggatcttga gccagagatc ggcgagccgg cgccaggccg ccgcgtcatt
     2881 cggcgcgacg ctgacgacct gcacatagat gtctgcggcg ctctcgacat cgccctttcg
     2941 cagcgcggcg tcggcgtctt tccggagcgt gatgaccggc ttctcgacgg tgccggcttc
     3001 atccttgaga tccgcctcga gcttgatcgc ggcgtcgtca agggcggcgt cttggaaggt
     3061 cttttcggcg gattggacgg gggtaagggg ggcggcggca aatacgaaca gcgcggcgaa
     3121 aaggccagcg cgaagccatg caggcatttt actcctcccg agcccgccct cggcgcggcg
     3181 ggacgagcca ataatgtagt ggcgctttgt gacggaagcg agagggtcga gagatcagtc
     3241 ggcaagaggg ggcaattccg gccagccata ggccgaagcg gcgcgcttga tcagggcttc
     3301 ggcgagcttt gagccattgt cggagttggt catcaacacc aatccctggc cgctcgccgg
     3361 gtaaaggatc aggtaaccct gatagccgac attttgggcg cgtttcatca gcacgaggga
     3421 ggcgccttcg ccggcaattg cggcgccgag gccgtaaggc ccaccattct ggggcgtcag
     3481 catctcttca agcgtctgcc ggcgaaggaa gatcgaggag aggccctgcc agacatcggc
     3541 aagctctacc aggagcttgg cgaggtcggt cggcgtcgac cacagccccg ctgcggcgtg
     3601 ctcaggaaag gcgtgccacc ttccgggcaa ttccgtgccg tcgctgaaat ggccgctcgc
     3661 ggcctttgcc gcgaaagccg cgctcggcgg ctgctcgaag ctgctcgccg tcatgcccat
     3721 ggggccgagc acgaggtctt gcatgagttg cgggaagggc tttcccgtcg cgtcctgcat
     3781 cagagcttcg gcgatctcat agcctccgcc ggaatagtga taggcgctgc cggggaccgc
     3841 gatctccgtg accggcggcg aatttgccgg cggggcgcca tcgagaattt gcccgagcgt
     3901 ggggaggggc gccccggcct cgtaaccgag gaaacctgga acgccgatgc cgccggtcat
     3961 gctgagcagc ccccgcaagg tgaccttgtg ggtcgcgtcg aacgcgttgc ctggcacgcg
     4021 ccaagaggtg agcttgtcgt tcacgccctg atcgagatcg agcgttccgt cttcgacgag
     4081 gcgcatggcg ccgatggcgg cgacgaattt cgacagcgat gccgcctgat agaccgtgtc
     4141 gggcgtcgcc tccttgccgt aggcgctggc aaaggcgatg cgtccctcat cgatcagcgc
     4201 gacgctggcg gagggaatgt cgagcagctt gagagcttcg tcgaggctaa cgtgccgctc
     4261 ctcgccttgc acagtgataa cgaggtcgtt ctcgatcgcg ccgggctcag ccgcctgcgc
     4321 tgcgctcgat gcaagcgaaa gaattgcgct gaagagcaag ctcaggaacg gggcacgtct
     4381 cttcatcctc atcctcgcgc cccgttcaac tcacgtcacg cggcccgccg ggcctcgcgg
     4441 gcgagcgtcg caatctcatc gcgcacccag ttgtggcatt cgtcacgatc gtaactcgaa
     4501 tgccagagga gacgcaccgg gaaggagacg ggatcgatcg gcagcttcac gcgggcaagc
     4561 gcgaactgct tctccaggat cgacgcgagc gaggctggaa gcgtgagcag cgcatcgctg
     4621 ttgagcgtga gcaatagtcc ggcaagcacc gatggaacct gcgccgagat gaagcaggtg
     4681 agtctctgct tggcgagctc gcggtcgagc agctcgatgg tgggaagcgc gctcaccgcg
     4741 atgtggtcgc tggcggcgaa ggagcgagca tcaggtttcc gctcgctcat ggggtgacct
     4801 gaacgggcga cgcatgcaag ccggtcgtca tagagcttga gcactcggat gtcgggcgcc
     4861 ggcagctcca tcgcctcgat gtcgatggcg aaatggcaac gcccctggcg gagcgcttcg
     4921 atggtctcct cgccctggcc gggcatccag ccgacgcgca ccgacggcgc caaggcctcg
     4981 agccgccgcc ggagcggctg caccagaatc agatcgccga cttcgctcgc ggtcatggcg
     5041 aagtcgcgct cggcgtcgcg tggatcgaag ggcttgcccg gacggagcag gctctccatg
     5101 ccggcgagaa tgacgtgcac ggtgggagca agctcctgcg ccttcgccgt cggcacgagg
     5161 cgctggccgg cgcgcacgaa cagctcgtcg gcgaagattt cgcgaagctt ggcaagggca
     5221 tggctgatgg cggacgcgct tcgccccagc cgttctgcgg cccgcgtgac gctcgcatcc
     5281 accagcagcg cgtcgaggat gacgaggaga ttggcgtcga cttctttcag atgcatgatt
     5341 tgaaataatt ctgctgaaaa gatttcacta cgtgcatgtt agcccgtgag ctaggttttt
     5401 tgcaaggatt caggctctag ggccgggacg cgcaatcggc gacccccgga tgcgcttcct
     5461 cattctcaac cttgcgacgg tcggagggct taagacccaa ggaccgtcgc acccttgttg
     5521 caaaggtcgt gggcttcttc ccccttcgat tgggataggg gctcggcggc caaggtgctt
     5581 acgtcgaggg atgcacatga tgcggatcgc ttcgcttgcc aggccggttg ctctcgttgc
     5641 gctcggcatt tgccttggat taccgccggc agccgccgac gagccgagcg ggaaggacgt
     5701 gctcaagacc tacgccgaca ttgccgcggc cggctatgcc gactctctcg ccacgacgag
     5761 gacgttgcag ctcgccgtca accagcttct cgccaatccg accgatcaga acatgctcgc
     5821 cgctcgcgcc gcctgggtcg cggcgcgcat cccctacatg cagaccgagg ccttccgctt
     5881 cggcaacaag atcatcgacg attgggaggg caaggtgaat tcctggccgc tcgacgaggg
     5941 cctgatcgac tacgtctcag gctcctacgg caccgactct cccgagaacg agctttacgc
     6001 tgccgacgtc atcgccaaca cgtcgctgac catcggcggc aagaagctga acacgtcgaa
     6061 gatcaccaag gagctgcttg ccgacaagct ccaggaggct ggcggcgtcg aggctaatgt
     6121 cgccaccggc tatcacgccg tcgagttttt gctctggggc caggacctga acggtaccgg
     6181 accaggcaac ggcaatcgcc cggcgagcga ctacaacgtc aagaaatgca ctggcggcca
     6241 ttgcgaccgc cgcgcgcagt atctccacgc cgccaccgat ttgctggtcg acgaccttgc
     6301 ctggatggca gcgcaatggg cccccggcgg cgaggcgcgc aagaccatcc aggacggctc
     6361 tgccgaggcc ggcctcaccg cgatcctcac cgggctcggc agtctctctt atggcgagct
     6421 tgccggcgag cgcatgaagc tcgggctgat gatccacgat cccgaggagg agcatgattg
     6481 cttctccgac aacactttcg cctcgcacta tttcgacgcg ctcggcatcc gcaacgtcta
     6541 tgtcggccgc tatcgacgcg ttgacggctc ggtggtgcag ggcccgagcg tgtcggacct
     6601 gatcaaggcg aagtcgcctg aggtcgataa cgcggtgagg gccaagatcg agctcaccat
     6661 ggacgccatg ggcgagctct accgccgcgg cgtcatggtc gagtcctacg accagatgat
     6721 tggcgagggg aatgaggtgg gctacgcgac cgtgcaaaag gtcgtggaca gcctgctcaa
     6781 tcagacgaag gagatcgagc gcgccgtggc cgctctcgat ctgaagtcca tcaagttcga
     6841 aggctcggac agcctggacg atccgaacaa ggtgaaagag gagaacaagt ccgccggcgt
     6901 cgcggcgccg gccaacgcgc agtaggtcgg aagacctatt gccggtgcaa ggcaggtaag
     6961 atgttataac ataacagaag ttccaaggag cctggccatc gagaagcccc ggagcggaac
     7021 gaactgacgt gagactgaaa gccagttggg gggctgaaat tgtgaagaga ccagagatcc
     7081 ggccattgtt gctggcgggc cgaagtctgc gggccatcgc acttggcggc atgcttgccg
     7141 ccggcctcgc gacactgcca agcgcggcac gggctgattg cgacgttccg gacaacggca
     7201 tccccgaaga gagcctgctg tcgaagctcg ggccggcctg ggtgagcctc ggcggcttgc
     7261 gcccggcgct tgccaagggc ggcatcggcg ttagcgccac ctattacggc gaagccttcg
     7321 ccaacagcgg cggcttcaag cagggcggca aatatgacgg cgtgctcggc gtcgccatcg
     7381 acgccgacct gcacgagctc ggcttctgga aaggcctctg cttccacacc aacggtttcc
     7441 agatccatgg ccagagcatc accgcctcca acatcgggag cctgatgccg gtgagcagcc
     7501 tcgaggccac gcccaacact cggctgttcg agctgtggct cgagcagcac atgttcggcg
     7561 acaagctggc ggtgaaagtt ggtcagctcg ccgccgacac cgaattcatc ctgtccgagg
     7621 ggggcggttt cttcttgaac ggcacctggg gctggccgtc gatcaccgcc gcagacttgc
     7681 cgagcggcgg tccggcctat ccgctggcca cgcccggcgt tcgcgtggcc gtcacgccaa
     7741 gcgacaagtt ccagcttttg gtcggtgtct ataacggcga ccctgctccg ccgtgcggca
     7801 gcgacgatcc gcaagtgtgc aacagcaacg gcctcgattt cgagctcgac gacccgccgc
     7861 tgctcatggt cgagggcgcg tacaagtaca atcaggaacg actcgccggc accgtcaagc
     7921 tcggcggctg gaaccatttc ggcgatttcg agcaccagcg cttctctgcc ggcggcaatc
     7981 tcatcgccgt gaccggggat cccggcaaac ccctcgacaa cgattggggc ttctacggca
     8041 tcatcgacca gctcatctgg cggctgcccg gcagcgagga ggcgaagggc gcggcgctgt
     8101 tcggccgcgt catcggcgcg ccgtccgacc gcaacctcat cgacttctac gccgatggcg
     8161 gcattacctt ctcaggcatg atccgtcgcc ggcccgacga cggcttcgcc attggcttcg
     8221 cctataccgg catttccgat caggtgcacg gcttcgacat cgattcaggg gctccggtgg
     8281 cgcgcaatta cgaggcgctg cttgagatct gctacaccat gcagcttgcc acgggctgga
     8341 ccttgcagcc tgacttccag tacatctggc agcccggcgg caatgtgcct gacgaagacg
     8401 gcagggccgt ggaaaatgcg gctgtgtttg gggcacgttc gaccatgagt ttttgagagg
     8461 tcaggttctg acgtaggccg attgcaccga aaccgttcca agtcccgctg tcgcgttgtc
     8521 ccagcactgt gtggagcatc gccgaagcca gtctcgccgc gtaagcgctc gcttgccgcg
     8581 ctgattgcgc ttggcgcgag cctgccgctg gtccttggcg cctgggagag ccagggcgcg
     8641 ggcgccgatg cgagcgtgcc cggcctcacc gcgccggcca ccaatttctc caagacagag
     8701 gagggcgagg acaagcccgg cggcggtgcc acctcgaagg cgagcgtgac caacgccaac
     8761 gccttctccg cctcctccgg caacatggac ttcaagcgcg agctcgactt caagatcggc
     8821 aatgccatct tccgcaaaaa ctgggtgtcg gcgccggctt cgaccgatgc ctccgacggg
     8881 ctcgggccgc tgttcaattc ccgcgcctgt cagaactgcc atctgaaaga cgggcgcggg
     8941 caccccccct tcacgaaggg catccccgac gacagccact cgatgctcat gcgtctttcg
     9001 gtcccagcca agaccgatga ggaaaaggcg aagcttgcgt cccatcggct gaactcgatc
     9061 cccgagccga cctatggcgg ccagcttcag gacctctcca tccagggtca taaggccgaa
     9121 ggccagatca agatcgacta caaggagaag cccgtgaagc ttgccggcgg ggagacggtg
     9181 catctcaggg tgcctcaata cagcatcgtc gatctcggct acgggccgct tgcgcccggc
     9241 accatgatga gcccgcgcgt agcgccgccg atgatcgggc ttggcttgct cgaagccgtg
     9301 cccgaggagc agatccttgc caatgccgat cccgacgatg caaatcagga cggcatctcg
     9361 ggcaagccca gccaggtctg gtcgcgcgag aacaacaagg tgatgctcgg ccgcttcggc
     9421 tggaaggcgg gcgtgccaag catcaaccag caggcggcgg aagcggccaa cggcgatatc
     9481 ggcctttcca ccacgatgat ccgcgcggca tcgggcgact gcaccggaag cgagaaggat
     9541 tgcctcgatg cgccgaacgg caattcgccg caatatcagg acgtcgagct tggcgacgat
     9601 ctgttcaagc tcgtcgcgtt ctattcccac aacctcgccg tcccgccgcg gcgcgatccc
     9661 gcagacccca aggtgctgaa gggcaaggag ctgttctatg cgatcggctg cgccagctgc
     9721 cataagccga aatttgtgac cggcgaagca cctggcgagc cgcatctctc ccaccagctg
     9781 atctggcctt acaccgatct cctgctgcac gacatgggcg aggggctcgc cgaccaccgc
     9841 cccgacggcg aggcgtcggg cagcgaatgg cgcacggcgc cgctctgggg catcggcctt
     9901 accgagaccg tcaacggcca tacgctcttt ctgcatgacg ggcgtgcccg ttccctcacc
     9961 gaggccattc tctggcacgg cggcgagggc caggcctcgc gcaacaaatt cgccaagctc
    10021 tccaaagagg accgggagcg cctgatcgct ttcgtgagct cgctgtgact ggcttcgctg
    10081 gaaatccccc tctcccctgt ggggagaggg ttgcggtgag gggggtctgt cagaaatcgt
    10141 ctcctcccgc acaccctcac ccgcgccgaa gcaaagcgct tcggcgcgac ctctccccac
    10201 cggggagagg tgtttggccc gtcgcagcca ttgcgtggat gcttcttcta tcgggcacca
    10261 tggcgctcca cgccgaggcg gatcacgccg ccatcgccaa gcaaagcctc gagcaataca
    10321 tcaggcctgg ctacgcgcat ctcgccgaga gcacggcggc tttgaaccag tcggtggcgg
    10381 cggtttgccg tacgccgtca gaagcggcgc tcaaagatgc gaaagaagcc ttcgccgcca
    10441 cggtgcaagc ctggagcctg gtcgagccca tccgcttcgg tccagtggcc gagcagcatc
    10501 gttatgagcg catcttctat tggccggacc ccaagggcct cggcgcgcgc caggtgcgcg
    10561 aggcgctcga caaagaggac gcgacggtaa ccgaagccgc gtcgctttcg gccaagagcg
    10621 tggcgctgca aggcctgccg gcgcttgaat atctccacta tggcgatgac gccggcactc
    10681 tggcaaaggg tggaggcaaa gccaccttcc gctgccgctt tgccgaaacc atcgccgcca
    10741 atctcggcgg catggccaag gacattgccg caggctggca ggatagcgcg ccttatgcca
    10801 aggcctatct cgagccaggt cacgacaacg cggcctatca cattcagaaa gaggtgacgc
    10861 tcgagctgtt caagaccttc actaccggca tcgagatggt gcgcgaccag aagatggcga
    10921 aggcgctggg agctaagccc gatcaggcaa ggccgcagct tgcgcccttc tggcggagcg
    10981 atttgagctt ctcaaacatg gcggacaatc ttcagagcgt gcgcgagctg ttcgccaagg
    11041 gcggcttcgc ggaagtggtg catgacgact cccctggggt cgaggattcg atcgtcttcg
    11101 acctcaatca cgccatcgag gtgttgcgtg ggctggacaa gccgatcacg caagccgtgc
    11161 acaacgagga ccttcgcgcc aagctcgaag ccttgcgcgt gtcgttgaag agcgccgcca
    11221 ccactgccgg cgacatcatc tcgcggagcg ccggcctcac tttcggtttc aatgccatgg
    11281 atggagactg accatgatca tcgaccgccg tgagcttctc atcggtgcgg cgagcagcgc
    11341 cgcgtctctc gctttgccgt ggcacgcaaa ggccgcgagt agcgaggaat tgttcgccgc
    11401 cgcccgccgc gacgatcgcg gcacctattc agcggcattg ttctcgctcg acggcggcga
    11461 tatccgcgcc gtcgagctac ctgagcgagg ccatgacatc gcgcttcgcc caggctctgg
    11521 cgaatgggtg gcgtttgccc gcaggcctgg ccgcttcggc gtcgccgtgc cgaccgacgg
    11581 gcgccagccg atctggttcc atgcgaaacc ggaccggcat ttcttcggcc atggcgtcta
    11641 ctcggccgac ggcaagctgc tctataccac cgagaacgac tatcagaacg cgcaaggcat
    11701 catcggcgtg cgcgacgcga ccgccttcta tcgccagatc ggcgagttcc cggcgcgcgg
    11761 catggaaccg catgacgtgg cgctgctcgc cgacggccgc accatggtga tcgccaatgg
    11821 cggcatcaaa acccaccccg actccggcgc cgacgagctg aaccttcccg acatgaagcc
    11881 ctcgctcgtc tatgtcgatc ttgccaccgg cgatcttgtc gaggagcaga ttctcgcacc
    11941 agagctgcac cagctttcca tgcgtcacct cgcgcttgcc gcgagcgata cggtggtgtt
    12001 cggctgccaa tatcgcgggc cggaggacga tgcgccaccg ctgctcggct ttcaccgccg
    12061 cggcgaaaga ccggtgatcg tgccggcgcc aaccggcacg caaacggcgc tcaagaacta
    12121 tatcggctcg gtgtcagccg atgccggcgg cggcatcgtc gccgcttcgg cgccgaaggg
    12181 cgggctgatc acctattggg acgttggcgc ccgccggttc ctcggctctt gcgagctgaa
    12241 tgacggctgc ggcattgcgc ccaccaggca caaagcaagc ttcctcctga ccagcggcca
    12301 gggctggctg gtcgagacca atgtcgctga cgcccccgag gaggaccgcc gctcttcgcc
    12361 ttacgaatgg gataaccacg ccattctcgt gcgctgaagg ccgcggccac acccttggcc
    12421 gacatggcgt gggcctattc tcaccttatt tctcggacta atctcggtcc gctaccccgc
    12481 gtcatggcca agcgttgcta ccggccattc acgtcgtcag cttcggcacc tacatagacg
    12541 ggaagtgggg gggcgcgttc ccggcatgac attgccgcaa ctttagtcag ctctctcggc
    12601 ggattgagcc tgatgcgaat gaaactgctg ctcaagatcg gcgcgggcgt cgtggcgctt
    12661 ctggtgctcg cgctggtcgc cttcaacctg cttatctcgg ccacggcggt gcgcgatcgc
    12721 gtggcggcgc gcgtcaagga gcagaccggc cgcgagctga ccgtcaacgg ctcgagctca
    12781 gttctcctga tgccgagccc gcatatcgtg ctcactgatg tgacgatcgt cgatcccgag
    12841 aaccgggcag gggccgatct gaaagtggcc cggctcgcgc tcgatgtgag cttcggccag
    12901 ctgttcagcc gcgaggtcga tgccaagcgc gtggtcatgg agcgcccggt gttcaccgtg
    12961 cggcccaaga ctcaagccgg cacgcagcag cagggcgatg ccggctcgac tccggagaag
    13021 cacgccgcgg cggagctccg cgtcattgcc gccggcgccg cctttgccgc cacgcgcgac
    13081 atcagcctcg acgacgttga gatcgaggac ggcacggtcc gcatcatcta cgacgagaac
    13141 ggcgccgagc ggcgcatcga aaagatcaat gccaatttga gcctgcccca cctcgtcgac
    13201 ccgctcaccg ccaagggcga cttcgattgg aagggcatcc gcgtcggctt cgacctcaag
    13261 ctcacctcgc cggccgatct cgagacgcgc tcggcaaggc tcgatcttgc cctcgatacc
    13321 gacgccatcg acgccaattt cggcggcaat gtctcgacta agccagcctt ctccgccgaa
    13381 ggcgatctca ccgccaagtc gcagtctgtg ccctcgctgc tcgcctggtt gcgcaagcag
    13441 ccgcccaccg agacggcggt cggcagcggc gagctgtcga gccatgtcgt ctggcagccc
    13501 ggcgagatct ccctcaccca ggtgcgcttt gcgctgagcc atgcgaccgg acaaggccag
    13561 gcggtggtca ccctgaagag cccaaggccg catctccgcg ccgcgcttgc gctggaaacg
    13621 ctcgatctca acccgctgct cgcgtccaac ggcgcccagg ctgcgcctca agcgccgtcg
    13681 gggccggcgc cgcccgcgac ggatagtgct gcccctgccg agccgtcgcc ttctacaggt
    13741 tctggagaga agggcgacaa tgagcgcgcc gcgacggaaa gcggcgaggc gcagggagag
    13801 agcaccgccc cgtcgccgga ggccggatcg ccaaaggcag accgaactgc tgccccgaac
    13861 gtcgcgccgg cggccttcga cgccgacgtc aatttgaatg tgcacgagac gaagatcttg
    13921 cagatgacca tcggtccaag ctcggccagt ctgtcgttgc gcgacggcgt gctcgacgcg
    13981 accttggcag atatgcagct ctatgacggg cagggcagcg gcaagttcac cctcgatgcc
    14041 tcgaagcccg ttccgacctt tgccggcaat ctcgcgctga acggcgtgtc ggcgcagccg
    14101 ctgctcaacg gcgcggcagg gttcaacctg ctcgccggca aaaccaacgt ggctctgcaa
    14161 ctgagcggca ccggaggcag cgaagatgaa gtgaagcatt cgctggctgg ccagggcagc
    14221 atcgcgatca gcgacggctc gatcgaaggc atcaatctga ccgagctgat ccagggcgtc
    14281 ggcgcgggcc agatgccgaa tctggagcaa ggccccggcg ccaagaccgc gttcagctcg
    14341 ttcggcgtca gcttcaacat cgcgagcggc gtcgccgaga ctcacgacct cgagatgacg
    14401 agcccgctgc tcaaagtcac cgggcgcggc accgtcgatg tggtgacgag cagcctcgat
    14461 ttcctcaccc agccggagat cgtcgccgga cctgaaggca aaggcggggc gaactctctc
    14521 gccggcctct ccatcccggt ccgcatcgag gggccgttcg accgcccgac cttcaagccc
    14581 gagatcaagt ccatgttctc aaaccccgag caggcttcga agaccatcaa ccagatcggc
    14641 gacgtgttgc agaagaagct caagggcaag ccggtgggcg aggccatcgg ccggattctt
    14701 ggcggggtgc gcatcggcaa cgagcgcagc aatggcggag gcgggcaaaa cgcaaagcca
    14761 ccggccaatc aggaagagcc ggatgaggag gggccgccgg aagagaccga tcagggccgc
    14821 gaccccgacc tcgacaatat actgcgctga gagccctcgg ccctaccctg acggcgccag
    14881 gcggcgcgaa atcttaaccc ttccggctaa ttctgagagc tgagtcaaat tggaacgagt
    14941 tccgcgaggg gcccgggggg tcgtcgttcg tgtttgtcgg gctgcgcagc gtcgcaattg
    15001 tgggtttgtg cgttgccgtc tcggccggtg cgggcagcgg cgtcgtggcg cgccagccct
    15061 acctcgccga cgtcgacccg catgagggcg tggcgcgcgc ccacgccatg ccggtgcagg
    15121 gcatcgacgt ctccaattgg cagggcgaca tcgactggaa caaggttcgc gccgccggca
    15181 cccaattcgc ctttatcaag gcgaccgagg gcggcgacta tctcgacccg aaatttgccc
    15241 agaactggca ggccgccaaa agcgtcggca tcccgcgcgg cgcctaccac atggtctatt
    15301 ggtgccgcgc cgctcacgag caggcgctgt ggttcatcct caacgtgccg aacgatcccg
    15361 acgctctgcc gccggtgctc gatctcgaat ggaacggcca ttcgcgcggc tgcccgaaac
    15421 ggattccggt gaagctcgcg ctcgagaaga tcaagatcat gctcgaggtc atggaggcac
    15481 acaccggcaa gcggccgatc atctacaccg atgtcgcgtt tcaccgcgaa gttctggaag
    15541 gcgagttgcc
//
LOCUS       DATNAE010000177         3122 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3477770, whole genome shotgun sequence.
ACCESSION   DATNAE010000177 DATNAE010000000
VERSION     DATNAE010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3477770"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="VN518_03920"
     CDS             <1..340
                     /locus_tag="VN518_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010914176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HXE03753.1"
                     /translation="AMKAGAVDFIEKPFDGTALLAAIRQALARSEQEARTLSQSADAA
                     ERIAQLTAREKEVLDELVLGHPNKVIAYNLSISPRTVEIHRARVMQKMEVRSLSALVR
                     LALEATKVGT"
     gene            423..782
                     /locus_tag="VN518_03925"
     CDS             423..782
                     /locus_tag="VN518_03925"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXE03754.1"
                     /translation="MSRPGPKRVIILDDDEAVRDSLAILLETHGYEVTSFASARAFLD
                     SGETGEDGCLILDLHLPDMSGFDVLRKLEGRMKVIMITARSDAATRQNALDAGAMTLL
                     EKPFTDELLVSTIQRAD"
     gene            932..1648
                     /locus_tag="VN518_03930"
     CDS             932..1648
                     /locus_tag="VN518_03930"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXE03755.1"
                     /translation="MLAAMTTQMMQRAENRSIAAPPAVAVQRKAAEVDPLARFGTVVT
                     LNSGEALFVEGDEAAYYYKVVSGAIRSYTLMPDGRRQIADFFVAGDFFGLTDHAVQSL
                     AAEAITAANLVRYRRHSVDEQAVSQPALARYLLDIACANLAAARHQMVLLGRKTAEER
                     IASFLLTLFERIGRVEREEEIIDLPMSRTDIADHLGLSTETVSRTFTSLARRGLLSLP
                     NAQTVVLCDREALQDIGAGF"
     gene            1688..2551
                     /locus_tag="VN518_03935"
     CDS             1688..2551
                     /locus_tag="VN518_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HXE03756.1"
                     /translation="MTIKTILTGASGGTAGEGTIDLALTLARRFSAHLEAIHVRTDMR
                     EILTATDGGDFMGASLTGQWIDQMEQEIATNAAKVKQAFAAATLRHDVPPGKQGAPGP
                     SVSFNDITGLSSIMLPERARFFDLVVLGRSERAIDRAHSDVIEQTLLHSGRPVLLAPA
                     TMSKTFGEVIAVGWDGSPQAVRAVTASMPFLEQARRALVITIGDTTGSDPAALVAYLA
                     RHEIAATHRFVAPVGGVGPGTQLLARAREEGAELLVMGGYGHAPWREFLFGGATQQAI
                     GQGLLPLFLVH"
     gene            2561..2959
                     /locus_tag="VN518_03940"
     CDS             2561..2959
                     /locus_tag="VN518_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03757.1"
                     /translation="MSWTRLRLELARGPERPEGSHRIGYELTLPLASDGHMAIEVFRE
                     LPELCTVHRFREDSEERVGALKHHRGDHWAFTYAGPGAFEENLPRFGQHRLRVGDYVS
                     VLDAHGRDHAYRIVSAVPAPGLTHVTMGAS"
BASE COUNT          508 a         1061 c          999 g          554 t
ORIGIN      
        1 ggcgatgaag gcgggcgccg tcgacttcat cgagaaaccc tttgacggga cggcgctcct
       61 tgccgcgatc cggcaggcgc tggcgcgttc cgaacaggag gcgcgcacgc tgagccagag
      121 cgctgacgcc gccgaacgca tcgcgcagct gacagcgcgc gagaaggagg tgctcgacga
      181 gctcgtcctt ggccacccca acaaggtgat tgcctacaat ctctcgatca gcccgcggac
      241 ggtggagatc caccgggccc gggtcatgca gaagatggag gtgcggagcc tgtccgcgct
      301 ggtccggctg gccctcgagg ctacgaaggt cgggacataa gcgttttccc ttacttttgc
      361 gaggcggtat tccgtgccat ctagtcgagc gggcaagcgg tgcccggtcg ttggagggcg
      421 atttgtcccg tccgggtccc aaaagggtga tcatcctcga cgacgacgag gccgtccggg
      481 attcgctggc aatcctgctc gagacccatg gctatgaggt gacgagcttc gcctcggcgc
      541 gcgcctttct cgacagcggc gagactgggg aggatggctg cctcatcctc gatctgcacc
      601 tgccggatat gagcggcttc gatgtgctgc gcaagctcga aggacgcatg aaggtaatca
      661 tgattacggc gcgcagcgat gccgcgaccc ggcagaatgc gctcgatgcc ggcgccatga
      721 cgctgctcga gaagcctttc accgacgagc tcctggtcag cacgatccag cgcgccgact
      781 agagcggcca ttctcgtttc tatcgcggga tgccgcgaaa ttcggccatt ttgccatagg
      841 tagaatcccg tacgtagctt ccctgactgc acggcactct tttccgcgcg tagatctggt
      901 cacggccgga acgctggaga tgggtgacga gatgttggca gccatgacta ctcagatgat
      961 gcagcgtgca gagaatcgca gcattgctgc gccgcccgcc gttgcagtcc aacgcaaggc
     1021 ggcggaggtc gatccgctgg cccgcttcgg cacggtcgtc acgctcaatt ccggcgaggc
     1081 gcttttcgtc gaaggcgacg aggcggccta ctattacaag gtggtgagcg gcgcgatccg
     1141 gtcctacacg ctgatgcccg atgggaggcg ccagatcgcc gacttcttcg tcgccggcga
     1201 cttcttcggc ctcaccgacc atgcggtgca aagcttggcc gccgaggcga ttaccgccgc
     1261 caacctggtg cgctatcgcc gccacagtgt cgacgagcag gcggtctcgc agcccgcttt
     1321 ggcgcgctac ctgctggaca tcgcctgcgc caatctcgcc gcggcgcggc accagatggt
     1381 gctgctcggc cgcaagacgg ccgaggagcg catcgcctcc tttctgctga cgctgttcga
     1441 gcgcatcggc cgcgtcgagc gcgaggagga gatcatcgac ctgccgatga gccgcaccga
     1501 catcgccgac catctcggcc tgtcgaccga aacggttagc cgcaccttca ccagcctcgc
     1561 tcgtcgcggc ctgttgtcgc tgcccaatgc ccaaaccgtg gtgctctgcg accgcgaggc
     1621 gctgcaggac atcggcgccg gcttctaacc gatagttttc ggcccgttcg ttcagggaga
     1681 agaagcaatg accatcaaga ccatccttac cggcgcgagc gggggcaccg ccggcgaagg
     1741 caccatcgat ctcgccctta ccctcgcccg ccgcttctcg gcgcatctcg aagccataca
     1801 tgtgcgcacc gatatgcgcg agatcctgac ggccacggat ggcggcgact tcatgggcgc
     1861 ctcgctcacc ggccaatgga tcgatcagat ggagcaggaa atcgcgacca atgccgccaa
     1921 ggtgaagcag gccttcgccg ccgcgacgct gcggcacgat gtgccgcccg gcaagcaggg
     1981 ggccccgggc ccgtcggttt ccttcaacga catcaccggc ctatcctcga tcatgctgcc
     2041 ggagcgcgct cgcttcttcg acctggtcgt gctcggccgt tctgagcgcg ccatcgatcg
     2101 cgcccattcc gacgtgatcg agcagacatt gctgcattcc ggccgccccg tgctgctggc
     2161 gccggccacg atgtcgaaaa cctttggcga ggtgatcgcg gtcggctggg acggctcgcc
     2221 ccaggcggtg cgcgccgtca ccgcttccat gccctttctc gagcaggcgc gacgtgcctt
     2281 ggtcatcacc attggcgaca ccaccggcag cgatccggcg gccctcgtcg cttatctcgc
     2341 ccgccacgag atcgcggcta cccaccgctt cgtcgcgccg gtcggcggcg tcgggccggg
     2401 cacgcagctc ctggcgcgcg cgcgcgagga aggggccgag cttctggtca tgggcggcta
     2461 cggccatgcg ccctggcgcg agttcctgtt cggcggcgcc acacaacagg cgattggcca
     2521 gggcctgctg ccgctctttc tggtgcattg aggccttgcc atgagctgga cccgccttcg
     2581 cctcgagctc gcgcgcggcc ccgaacgtcc ggaagggagc caccgtatcg gctacgagct
     2641 gaccctgccg ctcgcaagcg atggccacat ggccatagag gtgttccgcg agctgcctga
     2701 actgtgcacg gtgcatcgct tccgtgagga cagcgaggag cgcgtcggcg cgctcaagca
     2761 tcatcgcgga gaccattggg cctttaccta tgccggtccc ggtgctttcg aagagaatct
     2821 tccccgcttc ggccagcacc ggctccgtgt cggcgactac gtgtccgtgc tcgatgcgca
     2881 cggtcgcgat cacgcctatc gcatcgtctc cgccgtgccc gcccctgggc tgacgcatgt
     2941 aacgatggga gcctcttgat gaccatgatc gatacccgcc cagtgcctgc ggcgctcagc
     3001 aatcacgctg gcatccgcag cattctcgtg ccccttgcgg gacagccgga agaccgccgc
     3061 tgcctcaagg cagcgggcgc cttggcgcag cgtaccggcg cgcatgtcgc ggccctgtat
     3121 gc
//
LOCUS       DATNAE010000178         5147 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_1983728, whole genome shotgun sequence.
ACCESSION   DATNAE010000178 DATNAE010000000
VERSION     DATNAE010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5147
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1983728"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..909
                     /locus_tag="VN518_03945"
     CDS             154..909
                     /locus_tag="VN518_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009338001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HXE03758.1"
                     /translation="MTEKPGPSRDFSHVEAWVFDLDNTLYPSDCNLFAEIDTRMGDFI
                     AARFGVSLEEAQRMRKSYYYQYGTTLAGLMRLHDVCPNAFLDYVHDIDLSVIAPAPEL
                     GQALDALPGRKFVFTNGSRRHAESVIARLGLDGRFDDVFDIHAAEFIPKPERAAYERF
                     MGAHAVAAGRAAMFDDLPHNLRTAHSLGMTTVLVACGQTDHPEHRAIAGWGELPSHIH
                     HRTDALAPFLAEIGAMRAEEADRAAADGAYCCL"
     gene            929..1771
                     /gene="dapD"
                     /locus_tag="VN518_03950"
     CDS             929..1771
                     /gene="dapD"
                     /locus_tag="VN518_03950"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HXE03759.1"
                     /translation="MTHEHLKSFIESAYEHAGDISPKTKGVVRDAVEETLDLLDKGEA
                     RVAEKIDGAWVVNQWLKKAILLSFRLNDPHKVDGGPAGGAWWDKVPTKFANWKAKDFK
                     EAGFRAVPGAIVRRSAYIAPGVVLMPSFVNLGARVDSGSMVDTWATVGSCAQIGKNVH
                     LSGGAGIGGVLEPLQANPVIIEDNCFIGARAEVAEGMIVGEGSVLSMGVYLGQSTKIV
                     NRATGEVMQGRVPPFSVVVSGTMPSKLLPNGAPGPSLYCAVIVKTVDEGTRAKTSINE
                     LLRD"
     gene            1793..2308
                     /locus_tag="VN518_03955"
     CDS             1793..2308
                     /locus_tag="VN518_03955"
                     /inference="COORDINATES: protein motif:HMM:NF017470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HXE03760.1"
                     /translation="MSLTDLLFSFKGRINRKPWWLATIAAGLAASVITAIIQVIARFS
                     GHTAIDPVTNQVEPTGILGLLVGVIGLVNMWIAFALSAKRLHDRDRTGWWLIWQLLIL
                     ILAIILVVVAIAVPQEQGAVWYALAGAACLAAFVVSVWLFVQIGFLRGTQGPNRFGPD
                     PLGAARADAQL"
     gene            2380..3570
                     /gene="dapE"
                     /locus_tag="VN518_03960"
     CDS             2380..3570
                     /gene="dapE"
                     /locus_tag="VN518_03960"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014283879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HXE03761.1"
                     /translation="MTDLNETPVELARDLIRCPSITPVDAGALEVLERALARGGFHCR
                     RLTFTENGTPPVDNLFARIGGGSPHICFAGHTDVVQPGDLGSWTYPPFGGVVEDGVLY
                     GRGAADMKGAIAAFAAAAIDFAQAKDDDIPGTISLLITGDEEGLAINGTRKVLEWMAA
                     NGEQPDHALVGEPTNTTRLGEAIKIGRRGSLNGRITVTGMQGHVAYPHLAKNPLRGLI
                     AMLARLYDQPLDFGSAYFSPSNFEVTSIDVGNPTTNVIPVKAEAMFNIRYNDRHSADS
                     LQTKIRELAVKFLEPNGLDYELTFEPPSQAFLTEPGQLDSTLTQAVREITGITPSLAT
                     DGGTSDARFIKEMCPVVEFGLLTATIHKIDEQVSLADLAQLTAVYRRFLDLYFEEFGI
                     FELV"
     gene            3563..4381
                     /gene="murI"
                     /locus_tag="VN518_03965"
     CDS             3563..4381
                     /gene="murI"
                     /locus_tag="VN518_03965"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HXE03762.1"
                     /translation="MSDPRPIGVFDSGMGGLTVLRALAARLPQETFVYLGDTARLPYG
                     TKSPETVQAYALQATRLLIAEGVKMVVIACNTASAVALAPLAKALAPVPVIGVIEPGA
                     RAAVAATRNRRIAVIATEGTVKGEAYPRAIQALRGEMDVVQRPCQVFVALAEEGWTES
                     SVTAAAAKLYLGSLFEGSAAPDTIVLGCTHFPVLASAIRQVVGEDVAIVDSAETTAAA
                     VADALEAKGMSNETGATSSMRFFATDSPERFARVGAIFLGRPIDVADVELIDLL"
     gene            complement(4378..>5147)
                     /locus_tag="VN518_03970"
     CDS             complement(4378..>5147)
                     /locus_tag="VN518_03970"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HXE03763.1"
                     /translation="MSRRLAAGDTRGAALAQARGIQLALLMTVPSVAGAIAIPDLIMR
                     ALFARGAFTAADAASAGATLAAYAIGLLPFVMMRSFTAPFYARGDTLTPVKAALLAAA
                     VNIALKVVLMGPLAQVGLALATSAGAWINLTLLTIFARRRGFAVSGAGLRPVIRLFLA
                     GAVLAVVLYFGSIVLAKAFSGLPHFRQEITLAVLLLIGTIVYAATVLLLLGRDWLATL
                     FKDVGTAADTPPSKTLQPPDPTSASAALPDNTPPPNV"
BASE COUNT          905 a         1664 c         1699 g          879 t
ORIGIN      
        1 gagctgcctc gacgtcatcg ccgaaggggt tgaggcggtg gtgatcatca acggcaaggt
       61 gccacacgcc gtgctgctcg agctgttcac cgagcatggc gccggaacct taatcgagcg
      121 gcgcgcgcgc aaaggccgcc cgaggggatc gagatgaccg agaagccggg cccgagcagg
      181 gacttcagcc acgtcgaggc ctgggtgttc gacctcgaca acacgcttta tccgtccgac
      241 tgcaatctct tcgccgagat cgacactcgc atgggcgatt tcatcgccgc gcgcttcggc
      301 gtctcgcttg aagaggcgca gcgcatgcgc aaatcatatt actaccagta cggcacgacg
      361 ctcgccggcc tgatgcggct gcatgatgtg tgccctaacg ccttcctcga ttatgtacat
      421 gacatcgatc tgagcgtaat tgcgccggcg ccggagctcg gccaagccct cgacgcgttg
      481 ccagggcgca aattcgtctt caccaacggc tcgcgccgcc acgcagagtc ggtgatcgcg
      541 cggctcgggc tcgatgggcg cttcgacgat gtgttcgaca ttcatgccgc agaattcatt
      601 ccgaagccgg agcgcgcggc ttacgagcgc ttcatggggg cgcacgcggt ggccgcggga
      661 agagccgcca tgttcgacga tctgccgcac aatctccgca ccgcgcattc cctcggcatg
      721 accacggtgc tcgtggcttg cgggcagacc gatcatcctg agcaccgcgc catcgccggc
      781 tggggcgagc ttccctccca catccatcac cgcaccgacg cgctcgcgcc cttcctcgcc
      841 gagatcggcg ccatgcgcgc cgaagaagct gatcgcgccg cggctgatgg cgcctattgc
      901 tgcctttgag cgttgaggct gatccgacat gacgcatgag catttgaaga gcttcatcga
      961 gagtgcctat gagcacgccg gcgacatttc gccaaagacc aagggcgtgg tgcgcgatgc
     1021 ggtggaagag acgctcgacc tgctcgacaa gggagaggcg cgcgtggccg agaagatcga
     1081 cggcgcctgg gtggtgaatc agtggctgaa gaaggcgatc ctgctctcct tccgcctgaa
     1141 cgatccgcat aaggtcgatg gcgggcctgc gggcggcgcc tggtgggaca aggtcccgac
     1201 caaattcgcc aattggaagg ccaaggactt caaggaggcc ggcttccgcg cggtgccggg
     1261 ggccatcgtg cgccgctcgg cctatatcgc gccgggcgtg gtgctgatgc cgtccttcgt
     1321 caatctcggg gcgcgggtcg atagcggctc catggtcgat acctgggcca ccgtcgggtc
     1381 atgcgcgcaa atcggcaaga acgtgcatct ctccggcggc gccggcattg gcggcgtgct
     1441 cgagcctctg caagcgaacc cagtgatcat cgaggacaat tgcttcatcg gcgcccgcgc
     1501 cgaggtggcc gaaggcatga tcgtcggcga aggctcggtc ttgtcgatgg gcgtctatct
     1561 cggccagtcg accaagatcg tcaaccgcgc caccggcgag gtgatgcaag gccgcgtgcc
     1621 gcccttttcc gtggtggtat cgggaaccat gccaagcaaa ttgctgccga acggcgcgcc
     1681 tggtccgtcg ctttattgcg ccgtgatcgt gaagaccgtc gatgagggaa cccgcgccaa
     1741 gacctccatc aacgagctac tcagggattg aggcgggcgc gaggtgcaat ccatgagcct
     1801 gaccgacctc ctgttcagct tcaaaggccg catcaaccgc aagccttggt ggctcgcgac
     1861 catcgctgcg ggactcgcgg caagtgtcat caccgcaatc atccaagtca tcgcgaggtt
     1921 ctcaggtcac accgccatcg atccagtgac caatcaagtc gagccgaccg gaatccttgg
     1981 actgttggtg ggcgtcatcg ggcttgtgaa catgtggatc gcgtttgcgc tcagcgcgaa
     2041 aaggctgcac gaccgggacc gcaccggctg gtggctgatc tggcagctcc tgatcctcat
     2101 ccttgcaatt atcctggtcg tggtcgctat cgcggtgccg caggagcaag gggcggtttg
     2161 gtacgcgctt gccggcgccg cctgcctcgc ggccttcgtc gtttcagtct ggctgttcgt
     2221 gcagatcggc ttcctgcgcg gaactcaagg tccgaaccgc ttcggccccg acccgctcgg
     2281 cgccgcgcga gccgatgccc aactctagat cacgttcatc caaccctcgc tgccggtgcg
     2341 gcaagccgtt atacaaggcg ccagcaggca gggccgcaaa tgacagattt gaacgaaacg
     2401 ccggttgaac tcgcgagaga cctgatccgc tgcccgagca tcacgccggt tgatgccggc
     2461 gcgctcgagg tgctggagcg ggccttggcg cgcggcggct ttcactgccg gcggctgacc
     2521 ttcaccgaga acggcacgcc tccggtcgac aacctctttg cccgcatcgg cggaggttcg
     2581 ccgcatatat gctttgccgg tcataccgac gtggtgcagc cgggggactt aggctcgtgg
     2641 acctatccac ccttcggcgg cgtggtcgag gacggcgtgc tttatggaag gggcgctgcc
     2701 gacatgaagg gcgccatcgc cgccttcgcc gcggccgcga tcgatttcgc tcaagccaag
     2761 gacgacgaca ttcccggcac tatcagcctg ctcatcaccg gcgatgagga gggtcttgcg
     2821 atcaacggca cgcgcaaggt gctcgagtgg atggcggcca acggcgagca gcccgaccat
     2881 gctctcgttg gcgagccgac caacaccaca aggctcggcg aagccatcaa gatcgggcgg
     2941 cgcggcagcc tcaatgggcg catcacggtc accggcatgc aagggcacgt ggcctatccg
     3001 catctcgcca agaacccgct ccgaggactc attgcgatgt tggcgcggct ctacgaccag
     3061 ccgctcgatt tcggcagcgc ctatttctcg ccgtcgaatt tcgaggtgac cagcatcgat
     3121 gtcggcaatc ccaccaccaa tgtcattccg gtcaaggccg aggcgatgtt caacatccgc
     3181 tacaacgacc gtcactcggc cgactcgctg caaaccaaga tacgggagct cgccgtgaag
     3241 ttcctcgagc cgaatgggct cgactatgaa ctgacctttg agcccccgag ccaggccttc
     3301 ttgaccgagc ctgggcagct cgactcgacc ctgacccagg cggtgcgcga aatcaccggg
     3361 atcacgccgt cgcttgccac cgacggcggc acctcagatg cgcgcttcat caaggagatg
     3421 tgcccggtgg tggagttcgg gctcttgacc gcgaccatcc acaagatcga cgagcaggta
     3481 agccttgccg atcttgccca gctcaccgcg gtctaccggc gcttcctcga tctctatttc
     3541 gaggaattcg ggatcttcga gcttgtctga tcctcgcccg atcggggtct tcgactccgg
     3601 catgggcggg ctcacggtgc tgcgcgcgct ggctgcgcgg ctgccgcagg agactttcgt
     3661 ctatctcggc gacaccgccc gtctccccta cggcaccaaa agccccgaga cggtgcaggc
     3721 ctatgcgttg caagcgacgc ggctcctgat cgccgagggc gtgaagatgg tggtgatcgc
     3781 ctgcaacacg gcctccgccg tggcgcttgc cccgctggca aaagcgctcg cgccggtgcc
     3841 ggtgataggg gtgatcgagc ctggcgcgcg agctgccgtc gccgccacgc gcaaccggcg
     3901 catcgcggtg atcgccaccg aaggcacggt gaagggcgag gcctaccccc gtgccattca
     3961 ggccctcaga ggcgagatgg atgtggtgca gcggccttgc caggtgttcg tcgcgctcgc
     4021 cgaggagggc tggacggagt cctcggtcac ggccgccgcc gcgaagctct atctcggctc
     4081 gctgttcgag gggtcggcag cgcctgacac catcgtgctc ggctgcaccc atttcccggt
     4141 gctcgcttca gccatccgcc aggtggtcgg cgaggatgtg gcgatcgtcg actccgccga
     4201 aacgacggcg gcggcggtcg ccgatgcgct cgaagccaaa ggcatgagca atgagaccgg
     4261 ggccacgtca tccatgcgct tcttcgccac cgactcgccg gagcggtttg cgcgggtggg
     4321 agcgatcttc ttgggccgcc cgatcgacgt cgccgacgtg gagctgatcg acctcctcta
     4381 gacgtttggc gggggcgtgt tgtcgggcag cgccgccgac gccgaagttg ggtcaggcgg
     4441 ctgcagcgtt ttcgacggtg gcgtatcggc agcggtgccg acgtccttga acagcgtggc
     4501 gagccagtcg cggccgagca gcagcagcac cgttgccgca tagacgatgg tgccgatgag
     4561 gaggagaacc gcgagcgtaa tctcctgccg gaaatgaggg agccctgaga aggccttggc
     4621 cagcacgata ctgccgaaat agagcacgac ggcgagcacg gcgcccgcca ggaaaagcct
     4681 gatcaccggg cgaagccccg cacccgacac ggcaaagccg cggcgcctgg cgaagatggt
     4741 gagaagcgtc agattgatcc aggcgccggc gctggtcgca agcgcaagcc caacttgggc
     4801 gagcgggccc atcagcacca ctttcagcgc gatgttgact gcggcggcga gcagcgcggc
     4861 cttgaccggg gtcagcgtgt cgccgcgggc atagaacggc gcggtgaagc tccgcatcat
     4921 gacgaagggc aggagcccga tggcgtaggc cgcgagcgtg gcgccggcgg atgcggcatc
     4981 ggccgcggtg aaggcgccgc gggcaaacag cgcccgcatg atgagatcgg gaatggcaat
     5041 ggcgccggcg acgcttggaa cggtcatcag cagggccaat tggatgccgc gcgcctgggc
     5101 gagcgccgcg ccacgcgtgt cgccggcggc gaggcggcgc gacatct
//
LOCUS       DATNAE010000179         2029 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3477975, whole genome shotgun sequence.
ACCESSION   DATNAE010000179 DATNAE010000000
VERSION     DATNAE010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3477975"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1419
                     /gene="trpE"
                     /locus_tag="VN518_03975"
     CDS             <1..1419
                     /gene="trpE"
                     /locus_tag="VN518_03975"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HXE03764.1"
                     /translation="PVSAMLKLAHERPNSFLLESVEGGAARGRYSIIGCEPDLIWRAN
                     GARAELNRAPQADASAFAPSPLPTLQSLRALLAESAIRLPPKLPPMAAGIFGYMGYDT
                     VRLIEHLPATRPDGLDLPDSILVRPMMIVIFDTVKDEMTVVTPVRPAPGVSAKAAYSR
                     AADRLNVVIERLDQPLPHVSDLEAESAHPEPKPSVAPAAYMEKVARAKEYIAAGDIFQ
                     VVLSQRFEAPFTLPSFSLYRALRRVNPSPFLYYLNFDGFAVAGSSPEILVRVRDGIVT
                     IRPIAGTRPRGATPEQDKALANELLADAKERAEHLMLLDLGRNDVGRVAEVGSVRVTD
                     SFFLEYYSQVMHIVSNVEGRLDPRFDAIDALMAGFPAGTVSGAPKVRAMEIIDELEEA
                     KRGPYAGCVGYFSANGEMDSCIVLRTSVVKDGVMYVQAGAGVVADSVPEREQAECVSK
                     ASALFRAAEEAVRFAARAKRGQ"
     gene            1440..1952
                     /locus_tag="VN518_03980"
     CDS             1440..1952
                     /locus_tag="VN518_03980"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HXE03765.1"
                     /translation="MSLLRHAKSSWKDPTLADHERPLNTRGQAEAPVMGKAMTKHGLD
                     PDLVLCSTARRTRDTLALVLPELKTGPKVIYEDGFYHGTSAEMLGILHEVGAGIAQVL
                     LVGHNPELQEFALDLIGSGPKLLKDRLEAKYPTAGLVVLRFLTGAWKDVAINSGKLEL
                     FLTPGDVKKS"
BASE COUNT          354 a          670 c          653 g          352 t
ORIGIN      
        1 ccggtctcgg ccatgctcaa gctcgcccat gagcgtccga acagcttcct gctcgaatcg
       61 gttgagggag gtgctgcccg cggccgctat tccatcatcg gctgcgagcc tgatctgatc
      121 tggcgggcga acggcgcgcg cgccgagctg aaccgggcgc cgcaagccga cgcttcggcc
      181 ttcgccccct cgccgctgcc gacgcttcag tccttgcgtg cgttgttggc ggagtcggcg
      241 atcaggcttc caccgaagct gccgccgatg gccgccggca tcttcggcta catgggctac
      301 gacacggtga ggctgatcga gcatctgccg gcgaccaggc cggacgggct cgacctgccc
      361 gacagcatcc tcgtgcggcc gatgatgatc gtcatcttcg acacggtgaa ggacgagatg
      421 accgtggtca cgccggtcag gccggcgcct ggggtcagcg ccaaggcggc ctatagccgc
      481 gcggcagacc gcctaaacgt ggtgatcgaa cgcctcgacc agcctctgcc gcatgtgagc
      541 gatctcgagg ccgagtccgc gcatcctgaa ccgaagccca gcgtggcgcc tgcggcctat
      601 atggagaagg tcgcgcgcgc caaggagtac atcgccgccg gagatatctt ccaggtggta
      661 ttgagccaac gcttcgaggc gccgttcacg ctgccctcct tctcgctcta ccgggcgctc
      721 aggcgcgtca atccctcgcc cttcctctac tacctcaatt tcgacggctt cgccgtggcc
      781 ggctcgagcc cggaaattct ggtcagggtg cgtgacggca tcgtcaccat ccgcccgatc
      841 gcaggaacgc ggccgcgcgg ggcaacgccg gaacaggaca aggcgttggc caacgagctt
      901 ctcgccgatg ccaaggagcg cgccgagcat ctcatgctgc tcgaccttgg gcgcaacgat
      961 gtgggacgcg tggctgaagt cggcagcgtc cgcgtcaccg actctttctt cctcgaatat
     1021 tacagccagg tgatgcacat cgtgtcgaac gtggagggcc gcctcgatcc acgcttcgat
     1081 gccatcgatg cgctgatggc gggatttccc gccggcaccg tgtcgggcgc gccaaaggtg
     1141 cgggccatgg agatcatcga cgagctggag gaagcaaagc gcggacctta cgccggctgc
     1201 gtcggctatt tctccgccaa tggcgagatg gattcgtgca tcgtgctgcg cacttcggtg
     1261 gtgaaggacg gcgtcatgta tgtccaggcc ggagccggcg tggtcgccga ctcggtgcct
     1321 gagcgcgagc aggccgaatg cgtgagcaag gcatcagccc tattccgggc agcagaggaa
     1381 gccgtgcgct tcgccgcgcg cgccaagcgg ggtcaatagc tctcaccgcc atgctgacct
     1441 tgagcctcct ccgtcacgcg aaatcgagtt ggaaggatcc gaccctcgcc gatcacgagc
     1501 gccccctcaa caccagaggc caggccgagg cgccggtgat gggtaaggcc atgaccaagc
     1561 acgggctcga tccagacctc gtgctctgct ccacagcgcg gcggacgcgg gacacgcttg
     1621 ccctcgtgtt gcccgagctg aagaccgggc caaaggtcat ctatgaagac ggcttctatc
     1681 acgggacgtc tgccgagatg ctcggcatcc tgcacgaggt cggcgccggg atcgctcagg
     1741 tgctgctcgt cgggcacaat ccggagctcc aggaatttgc tctcgatttg atcggctcgg
     1801 gtcccaagct tctcaaggat cgcctcgagg cgaagtaccc gaccgcgggg ctcgtggtgc
     1861 tccgcttcct taccggcgcc tggaaggacg tcgccatcaa cagcggcaag ctcgagctgt
     1921 tcctgacgcc gggcgacgtg aagaagagct gaccaaactt gacggcgcgc gggaagcggg
     1981 cgtaacaccc tgcctatgct cacgctgatc gacaactacg acagcttta
//
LOCUS       DATNAE010000180         5376 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2580055, whole genome shotgun sequence.
ACCESSION   DATNAE010000180 DATNAE010000000
VERSION     DATNAE010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5376
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2580055"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VN518_03985"
     CDS             complement(<1..263)
                     /locus_tag="VN518_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03766.1"
                     /translation="MDRRSAAPPALYYYLGLHPEIFMSREKELNFFMEERNWHRGLDW
                     YRAQFETSRSVSVWGEASPGYTHHPIFPGVPRRMHSVVPRARL"
     gene            complement(314..1282)
                     /locus_tag="VN518_03990"
     CDS             complement(314..1282)
                     /locus_tag="VN518_03990"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HXE03767.1"
                     /translation="MVHPSNLTTVPDLSRKPGLRLVVLLNAGAGTASHRGADGLRGDL
                     EAALKALGVSADLEICSGPDLKRLAEAALARARSAEIDAVVVGGGDGSIRTVAGVLAE
                     TGVPLGILPLGTLNHFAKDLGIPLHLKDAAEVIAKGDTRTVDLAEVNGETFINNSSIG
                     IYPYIVLDRERRRANHKLAKWMAMVPALFRVLKHFPRRQLALSAEGWTRPYRTPCLLI
                     GNNEYGMEFFELGRRHHLDRGEISVYVVKQRRPFGFFWMICRMVLGKVSQARDIESFR
                     LKELQVRSKTSRLPVALDGEVEMMHPPLHYRTRPGALRVIVPGESR"
     gene            complement(1328..2191)
                     /locus_tag="VN518_03995"
     CDS             complement(1328..2191)
                     /locus_tag="VN518_03995"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HXE03768.1"
                     /translation="MRTLLLHNPTAGNGCLSADSLEAALSRSGCLVTACSKHDDSYKA
                     ALGKPWDLVAVAGGDGTVAKAARHTPDRNVPLALLPIGTANNVAHSLDLLGEAEEIAA
                     NLQGAPTRPLDLGSVKGPWGERSFIEAVGLGAFAPAITRSDPKPPFEHRIRLGREALC
                     EAIGAAEPHTIALTVDGEPLDGEFLFVEVLNLRFSGPRLPIAYFAEPGDQMLDIVLLC
                     EDQRQAMLDWVRSTPEETPPPVLVHIGRNVKMAWDGAPLRLDDRVCPPKDGPHVIDIT
                     LEQDSLRVLCP"
     gene            complement(2269..3117)
                     /locus_tag="VN518_04000"
     CDS             complement(2269..3117)
                     /locus_tag="VN518_04000"
                     /EC_number="2.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HXE03769.1"
                     /translation="MSRTVYVNGSFVPEADAKISVFDRAFLFAEGVYEVTAVLGGKPL
                     DLENHLARLDRSLAEMPIDPPLSHAELRALHAELIALNKVDEGVVYLEITRGVADRDF
                     LYPEGVPPTVVAFTQSRPLLDNPHAETGIKVITLPDIRWKRRDIKSTALLAQTMGKQE
                     AKRRGAYEAWMVENGFVTEGTSSTAFILDVNGTIRTQPLGHHILPGVTRRAVLHLAQQ
                     EGLAIEERPFTVAEAMQAREALLTAASAFVLPVVEIDGKPIGDGRPGRVAREFRRLYI
                     EEARKG"
     gene            complement(3262..4287)
                     /gene="bioB"
                     /locus_tag="VN518_04005"
     CDS             complement(3262..4287)
                     /gene="bioB"
                     /locus_tag="VN518_04005"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010683735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HXE03770.1"
                     /translation="MNGKAHQAETAGESASATRGPRHDWQKSEVVALLTQPLLDLVDQ
                     AREVHRRHHADGTVQLASLYSIKTGACPEDCKYCPQSAHYAKTTGLKREESLDVDEVL
                     SKAAIAKEAGATRFCMGAAWREVKDGEAFDRVLAMVRGVRALDMEACVTLGMLNQSQA
                     DRLAEAGLTAYNHNLDTSAEFYGEIITTRCYQDRLDTLERVRRAGVEVCCGGIIGMGE
                     SVEDRAGLLQTLANLDPHPESVPINALVAVPGTPLQDRPPIDPLDLVRMVAAARVLMP
                     ASRVRLSAGRRSLSREAQLLCFLAGANSIFYGDKLLTTANNDVSDDLALIEEAGLRVQ
                     EPEAIGV"
     gene            4369..>5376
                     /locus_tag="VN518_04010"
     CDS             4369..>5376
                     /locus_tag="VN518_04010"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HXE03771.1"
                     /translation="MPGSERSYGAYLEARERDGLRRGLTGIEARDARTVRIGGEAYVN
                     FASNDYLALRFHPALIKRAKAFAEAHGVGSGASRLVTGNLDLFAGIEVKVAALKQKPT
                     ALIMASGFQANASILQALFDRSVLGAEPLVFSDRLNHASIHFGCAASGARETRYRHGD
                     AAHLAALLSQYQGDERPKFILTESVFSMDGDIAPLAEIGRLAREHDAMLIVDDAHATG
                     ILGDGGRGLSGEADVVIGTFSKALGGFGAYVACSDTLRDYLINRCSGLIYSTALPPPV
                     LGAIDAALDLVPSLDAERAHVAKLAERFRLGARALGFDTGASTTQIVPLIAGANHAAL
                     SL"
BASE COUNT          967 a         1694 c         1744 g          971 t
ORIGIN      
        1 atgagccggg ctctcggaac cacggaatgc atccgccgcg gcacgcccgg aaaaatcgga
       61 tggtgggtat agccgggcga agcctcaccc cagacgctga ccgatcggct cgtctcgaac
      121 tgcgcccgat accaatcgag ccctcgatgc cagttccttt cttccatgaa gaaattcagc
      181 tccttctcgc gcgacatgaa aatttcggga tgcagaccga gataataata gagggctggt
      241 ggtgccgcac ttcggcgatc caatgatgac gagatttggt aactgggcca tgcgcagcgc
      301 ggcgaacctc gaactaccga ctttcccccg gcacgataac gcggagcgcc ccaggccgcg
      361 tgcgatagtg gagcggaggg tgcatcatct ccacttcgcc gtcgagcgcg acaggcagcc
      421 ggcttgtctt ggagcgcacc tgcagctcct tcaagcggaa gctttcgatg tcgcgcgctt
      481 ggctcacctt gccgagaacc atgcggcaga tcatccagaa gaagccgaac ggccggcgct
      541 gcttgaccac atataccgag atctcgccgc gatcgaggtg gtgacgccgg ccgagctcga
      601 agaactccat gccgtattcg ttattgccga tgagcaggca cggcgtgcgg tagggccgcg
      661 tccagccttc cgccgagagc gcgagctggc ggcgcggaaa atgcttcagc acgcggaaca
      721 gcgccggcac catcgccatc catttggcaa gcttgtgatt ggcccttcgg cgctcacggt
      781 cgagcacgat ataggggtag atgccgatcg aggagttgtt gatgaaggtc tcgccgttga
      841 cctcggcgag atcgacggtg cgtgtgtcgc ccttggcgat gacttcggcg gcgtccttga
      901 ggtgcaaggg gatgccaagg tctttggcaa agtgattcaa ggtgccgagc ggcaggatgc
      961 cgagaggaac gccggtctcg gcaagcacgc cggccacggt gcgaatgctg ccgtcgccgc
     1021 cgccaaccac cactgcgtcg atctcggctg atcgtgccct ggccaaggcc gcttcggcca
     1081 agcgtttcaa gtccgggccg gagcagattt cgagatctgc cgagacgccg agcgccttga
     1141 gcgcggcctc caagtcgccg cgcaggccat cggcgcccct gtggctcgcg gtgcccgcgc
     1201 cggcgttcag gagcacgacc agcctcaggc ctggctttcg tgataggtcg ggcactgtcg
     1261 tcaggttgct tggatgtacc attggacagg gcatcccacg ccggcgccgt ctgtgcaacg
     1321 gctacgctca agggcagaga acgcgaaggc tatcctgctc aagagtgatg tcgatgacat
     1381 gcgggccgtc cttgggcgga cagacgcgat catcgagccg caaaggcgca ccgtcccagg
     1441 ccatcttcac gttccggcca atgtgcacca agaccggcgg cggcgtctct tccggcgtac
     1501 tgcggaccca gtccaacatc gcttgtctct ggtcctcgca aaggagaaca atgtcgagca
     1561 tctgatcccc tggctcggcg aaataggcga tggggaggcg gggaccgctg aagcgcaaat
     1621 tcagaacctc gacgaatagg aactcgccgt cgagcggttc tccgtccacg gtcagcgcaa
     1681 tcgtgtgcgg ctccgccgcg ccaatcgcct cgcacaaggc ctcgcggccg agtctgatgc
     1741 gatgctcgaa cggaggcttg ggatcggagc gcgtgatcgc aggagcaaag gctccgaggc
     1801 cgacagcttc tatgaaactg cgttcgcccc atggcccttt gaccgatccg aggtcgagcg
     1861 gacgcgtggg agctccctgc agattggctg cgatctcctc agcctcaccc agcaagtcga
     1921 gcgaatgcgc cacgttgttg gcggtgccga tcggcagcag cgcgagggga acgtttcggt
     1981 ccggcgtatg gcgcgcagcc tttgccaccg tgccgtcgcc gcccgcgacg gcaacgaggt
     2041 cccagggctt gccaagtgcg gccttgtagc tgtcgtcgtg ctttgagcag gcggtgacca
     2101 aacatcccga ccgtgacaga gcagcttcca agctgtcggc gctcaagcat ccgttgcccg
     2161 ccgtcggatt gtggaggagt agggtgcgca tcgctgctcc cgctgatgat gaaagccccg
     2221 tcgtggagag atcatcaacg ccgaagctgt ggcacggttc cggaagaatc agcctttgcg
     2281 cgcttcctcg atgtaaagac ggcggaactc gcgcgcgacc cgccccggcc gcccgtcgcc
     2341 gatcggcttc ccgtcgatct caactaccgg cagcacgaag gccgaggcag cggtgagcaa
     2401 ggcctcgcgg gcctgcatcg cttccgccac cgtgaagggc cgctcctcga tggcgagacc
     2461 ttcttgttgc gcaaggtgca acacggcgcg gcgcgtcacg ccggggagga tgtggtgacc
     2521 gagcggctgg gtgcggatgg tgccgttcac atcgagaatg aaggctgtcg aggacgtgcc
     2581 ctcggtgacg aagccgtttt cgaccatcca ggcctcgtag gcgccgcgcc gcttcgcttc
     2641 ctgcttgccc atggtctggg caagaagcgc ggtcgatttg atgtcgcgcc gcttccagcg
     2701 aatatcgggg agggtgatga ccttgatccc ggtctcggca tgagggttgt cgagcaaggg
     2761 ccggctttgg gtgaaagcga ccaccgtggg tggcacgccc tcaggataga gaaagtcgcg
     2821 atcggccacg ccgcgggtga tctcgagata gaccacgcct tcatcgacct tgttcagcgc
     2881 gatcagctcg gcatgaagcg cgcgaagctc ggcatggctg agcggagggt cgatcggcat
     2941 ctcggcgagc gagcggtcga gccgcgccag atgattttcg aggtcgagag gcttgccgcc
     3001 caagaccgcc gtcacctcgt acacgccttc ggcgaacagg aaagcgcggt cgaagaccga
     3061 gattttggcg tcggcctccg gcacgaagct gccattcaca tagacggtgc gggacatggc
     3121 cgaggtaatc atccgcggga gtgaggcgag acgctggccc tccacgtcat accggcgaaa
     3181 gccggtatcc agcaaacaaa tcaaaaacgg tgggctggat tccggcttgc gccggaatga
     3241 cggagcatgt tgtgacttga gtcaaacccc gattgcctct ggctcttgca cgcgcaagcc
     3301 cgcctcttcg atcagggcaa gatcgtcgct cacgtcgttg ttggcggtgg tgagcagctt
     3361 gtcgccgtag aagatcgagt tggcgccggc gaggaagcag agcaattgcg cctcccggga
     3421 aagcgagcgg cgccctgccg agagccgaac gcgggacgcc ggcatcagca cccgcgccgc
     3481 agccaccatg cgcacgaggt cgagcggatc gatcggcggc cggtcttgca gcggcgtacc
     3541 tggcacggcg accagcgcgt tgatcggcac gctctcggga tgcggatcga gattggcgag
     3601 ggtttggagc agcccagcac ggtcctcgac gctctcgccc atgccgatga tgccgccgca
     3661 gcagacctcg acgcctgcgc gccgcacacg ctcgagcgta tccagccggt cctgatagca
     3721 gcgcgtcgtg atgatctcgc cgtagaactc cgccgacgta tcgagattgt ggttgtaggc
     3781 ggtgagcccg gcctctgcta ggcgatcggc ctgggactga ttaagcatcc cgagggtgac
     3841 gcaagcctcc atatcgagcg cacgcacgcc gcgcaccatg gcaagcacgc ggtcgaacgc
     3901 ctcgccgtcc ttcacctcgc gccacgctgc gcccatgcaa aagcgcgtgg cgccggcttc
     3961 tttggcgatg gcggccttgg acagcacctc gtcaacatca aggctctctt cgcgcttcag
     4021 cccggtggtc ttggcgtaat gggccgattg cgggcaatat ttgcagtctt ccgggcaggc
     4081 cccggttttg atcgaataga ggctggcgag ctgaaccgtg ccgtcggcgt gatggcggcg
     4141 gtgcacctcc cgcgcctgat cgaccaggtc gagcaaaggc tgagtcagaa gggccacgac
     4201 ctcgcttttc tgccaatcgt ggcgggggcc gcgagtcgcg gaggcggatt caccggctgt
     4261 ctccgcctga tgagccttgc cattgatagg attcgggaaa gttggaagct cggtcacgac
     4321 ttcgcgttgc gattgctttc gggtccagcc cttcgctaat acaggatcat gccaggaagc
     4381 gaaagaagct acggagccta tcttgaagcg cgcgagcgcg atggcctgcg ccgcggcctc
     4441 accggcatcg aggcgcgcga cgcaagaacc gtccgcatcg gcggcgaggc ttacgtcaat
     4501 ttcgccagca acgactatct ggccttgcgc tttcatccgg cgctgatcaa gcgggcgaaa
     4561 gctttcgcgg aagcgcatgg cgtggggtcc ggcgcttccc ggctcgtcac cggcaaccta
     4621 gatttgtttg caggtatcga agtcaaagtc gcggcgctga agcagaagcc gacggcgctg
     4681 atcatggcct ctggcttcca ggccaacgcc tcaatcttgc aggcgctgtt cgatcggagc
     4741 gtgctcggcg ccgagccact cgttttctcc gaccggctca atcacgcgag catccatttc
     4801 ggctgcgccg cctctggcgc ccgagagacc cgctaccgcc atggcgacgc tgcccatctc
     4861 gctgcgcttc tgagtcaata tcaaggggat gagcggccga aattcatcct gaccgagagc
     4921 gtgttctcca tggacggcga tattgcgccg ctcgctgaga ttgggcgtct cgcccgtgag
     4981 catgacgcca tgctcatcgt cgatgatgcc cacgccaccg ggatcttggg cgacggcgga
     5041 cgagggttgt cgggcgaggc ggatgttgtc atcggcacct tctcaaaggc acttggcggc
     5101 ttcggcgcct atgtcgcctg ctcagacact ttgcgcgact atctgatcaa tcgctgttcc
     5161 gggctgatct attccacggc gctgccgcct cccgtgcttg gcgccatcga cgcagctctc
     5221 gatctcgtgc cgtcgctcga tgccgagcgc gctcatgtcg cgaaactcgc cgagcgcttc
     5281 cgcctcggcg cgcgagccct cggattcgac accggcgcct cgacgaccca gatcgtgccg
     5341 ctgatcgcag gagccaatca tgcggcgctg agcttg
//
LOCUS       DATNAE010000181         4210 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2888955, whole genome shotgun sequence.
ACCESSION   DATNAE010000181 DATNAE010000000
VERSION     DATNAE010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4210
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2888955"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="VN518_04015"
     CDS             <1..532
                     /locus_tag="VN518_04015"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HXE03772.1"
                     /translation="RRRARAARLARVGAKRRAAPTSLTPRPPRLAGGAAARRGEPPDA
                     RSQRKGHVWRTAASPCLRIDSYVQAGLAAARWAHAEIFGRLGATGIEHRDAPEGAGHI
                     MGTARMGNDPKSSVVDRDLRNHDHANLFILGSAVFPTGATAKSDADHRRAQPTFGRAC
                     QSGIVAVIRTLFGARR"
     gene            558..1031
                     /locus_tag="VN518_04020"
     CDS             558..1031
                     /locus_tag="VN518_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXE03773.1"
                     /translation="MVRVVDIDHLVISVRDFEKSKSFYAPLMKFLGFDVEAEYEGSMM
                     GWANGNTLFWIAAADAEGKKHTYRKGDIGFHHYAFRLRSRADVDALQAYLEEQSATIV
                     DPAGEYYDDYYAVFFLDPDGMKLEGMRYGERHAKAARARAARKKTAAVKRKTTKR"
     gene            complement(1028..1456)
                     /locus_tag="VN518_04025"
     CDS             complement(1028..1456)
                     /locus_tag="VN518_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HXE03774.1"
                     /translation="MVDQILAGGATSLVNLVIHALVMGALVKLLLGMAGRGSGAPEFM
                     RYTVVIVTTGSVLFAAHFAEILLWAYTYAAVGAGPPGTDLVYFAFSNYTTLGYGDILP
                     VDHWRLLAPMTALNGIMLIGWSTALIFAFLRRTVPQLEKH"
     gene            complement(1467..2684)
                     /gene="rmuC"
                     /locus_tag="VN518_04030"
     CDS             complement(1467..2684)
                     /gene="rmuC"
                     /locus_tag="VN518_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HXE03775.1"
                     /translation="MALEPIQVGSVTLDPVLVLLGLLGFAALALFVLVVFVAVHMSRR
                     RREVRESDAQLAELKGRLQTLAEISISRHGELARAVNERLDRMTHRVGSDLTETARKT
                     TDSIAKLHERLAVIDTAQKNLTELSSNMVSLQEILANKQARGAFGQMRMEAIIQDGLP
                     KGAFTFQATLSNGKRPDCLLHMPNTKAGVVIDAKFPLEGFEAFRVARGPEEKKEAARR
                     VRVDVGRHVDAMAERYFLPGETQDTAILFVPSEAIYADLAEHFSDLVQKAHRARIVIC
                     APNMLMLAVQTMQAILKDVKMREQAHLIQREVSRLMEDMGRLRERVLDLQRHFGQAND
                     DIEKILTSSERIASRGHKIDNLEFEGDGANGVEVDRAAPLVAQSAARHPAENGGERRR
                     PLVTRQPDLLAGE"
     gene            2774..3289
                     /gene="def"
                     /locus_tag="VN518_04035"
     CDS             2774..3289
                     /gene="def"
                     /locus_tag="VN518_04035"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003583874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HXE03776.1"
                     /translation="MAILPIITIPDPLLRKKAAPIERVETALLKLAENMLATMYDAPG
                     IGLAAPQVGILRRLIVMDPSRDEEAKQPLVMVNPVILERGSEMRTHEEGCLSIPDVTA
                     EIERPAVTRVGFIDREGKSQEQTLEGIWSTLVQHEIDHLNGVLFIDYLSRLKRDMIVK
                     KFTKQKRADAL"
     gene            3345..>4210
                     /gene="fmt"
                     /locus_tag="VN518_04040"
     CDS             3345..>4210
                     /gene="fmt"
                     /locus_tag="VN518_04040"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006172160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HXE03777.1"
                     /translation="MRIIVMGTPDFAVPALGAVLAAGHEVVGVYTQPPRAAGRGLAAR
                     KSRVQLFAESRGLPVHTPVSLRSAEEQARFKALNADAGVVVAYGQMLPKAILEAPHYG
                     CFNIHASLLPRWRGAAPIQRALLAGDAATGVAVMRIEEKLDTGAVCLMERTAIGPDET
                     AGELHNRLSELGAELMVRVLSDLGKGSLACWPQHEQGVTYAAKIEPGETRIDWSRPAR
                     DVHNLIRGLSPHPGTWFEIDLNGKRERVRALRSTLAERGGEPGTVLDDQLTIACGKGS
                     VRLLQMQRAGKRP"
BASE COUNT          785 a         1382 c         1339 g          704 t
ORIGIN      
        1 ccgccgccga gctcgcgcgg caaggcttgc gcgggtcgga gctaaacgcc gcgctgcgcc
       61 gacaagcctc acgccacgtc cgcctcgcct cgcaggtgga gcagctgccc gacggggaga
      121 accgcctgac gctcgatccc aaagaaaagg acatgtatgg cgtaccgctg cctcgccttg
      181 cctacgcatc gattcctatg tccaggctgg ccttgctgcc gcgcgttggg cgcacgccga
      241 gatcttcggc aggctcggcg ccacggggat cgagcaccgc gacgcgccgg aaggcgccgg
      301 ccatatcatg ggcacggcgc gcatgggcaa cgatcccaaa agctcggtgg tcgaccgcga
      361 cctgcgcaac cacgatcacg ccaacctctt catcctcggc tcggccgtgt tccccaccgg
      421 cgccacggcc aaatccgacg ctgaccatcg ccgcgctcag cctacgttcg gccgggcctg
      481 tcaaagcggc attgtcgcag tgatccgaac cctcttcggg gcgaggcgtt gaatcaccac
      541 aaccaaggga gaagcacatg gtgcgcgttg tcgacatcga ccatctggtg atcagcgtca
      601 gagactttga aaagtcgaag tccttctacg cgcctctgat gaagttcctc ggttttgacg
      661 tcgaggccga atatgaaggc agcatgatgg gctgggccaa cggcaacacg ctgttctgga
      721 tcgccgcagc cgacgccgag ggcaaaaagc acacataccg caaaggcgat atcggcttcc
      781 accactacgc cttccgcttg cgcagccggg ccgatgtcga tgcgctgcaa gcgtatctcg
      841 aagagcagag cgcgacaatc gtcgatcccg ccggcgaata ttacgacgac tactacgcgg
      901 tgttctttct cgaccccgac ggcatgaaac tcgaaggcat gcgctacggc gagcgccatg
      961 cgaaagccgc ccgagcccgc gctgcgcgca agaagaccgc ggcggtgaag cgcaagacga
     1021 cgaagcgcta gtgcttctcc aattgcggca ccgtccggcg caagaatgcg aagatcagcg
     1081 ccgtcgacca gccgatcagc atgatgccgt tcagcgccgt catcggagca agaagccgcc
     1141 aatggtcgac cggcaggatg tcgccatagc cgagcgtcgt gtagttgctg aaggcgaaat
     1201 agacgaggtc agtgccgggc ggccctgcgc cgaccgcggc ataggtatag gcccagagca
     1261 ggatttccgc gaaatgggcg gcgaacaaaa cgcttcccgt ggtgacgatg acgacggtgt
     1321 agcgcatgaa ctctggggcg ccggagccgc gccccgccat gccaagcagg agcttgacca
     1381 gcgcgcccat aaccaaggcg tgaatcacaa gattgacgag acttgtcgcg ccgccggcga
     1441 gaatctgatc gaccatcgct acaagactac tccccggcga gcagatcggg ctggcgtgtg
     1501 acgagaggcc gccgcctttc tcccccgttt tccgccggat ggcgcgccgc tgattgggca
     1561 acaagcggcg ccgcacggtc aacttcgacc ccgtttgccc cgtctccctc gaactccaag
     1621 ttgtcgatct tgtggccgcg cgaagcgatc cgctccgacg atgtgaggat tttctcgatg
     1681 tcgtcgttcg cctggccgaa atgccgctgt aggtcgagca cgcgctcgcg caaccgcccc
     1741 atgtcctcca taagcctgct cacctcgcgc tgaatgagat gggcctgctc gcgcatctta
     1801 acgtctttca ggattgcctg catggtctgc accgcaagca tgagcatgtt cggcgcgcag
     1861 atgacgatac gggcgcggtg cgccttctgc acgagatctg agaaatgctc ggcgagatcg
     1921 gcatagatgg cctccgacgg cacgaacagg atcgcggtgt cctgggtttc tcccggcagg
     1981 aaatagcgct cggccatggc atcaacgtga cgcccgacat cgacgcgcac gcgccgcgcc
     2041 gcctcttttt tctcctccgg cccgcgcgca acgcggaacg cctcgaaacc ttccagcgga
     2101 aactttgcgt cgatcaccac gccggccttg gtatttggca tatgcaagag gcagtcggga
     2161 cgcttgccgt tggagagcgt ggcctggaag gtgaaggcgc ccttgggcaa gccgtcctgg
     2221 atgatggctt ccatgcgcat ctggccgaag gcgccgcgcg cctgcttgtt ggcgaggatc
     2281 tcctgaaggg agaccatgtt ggaggaaagc tcggtcaggt tcttctgcgc cgtatcgatg
     2341 acggcgagcc tctcatgcag cttggcgatc gagtcggtgg tcttgcgcgc ggtctcggtc
     2401 aggtctgagc cgacccgatg ggtcatgcgg tcgagccgct cattgacggc gcgggcaagc
     2461 tcgccgtgac gcgagatgct gatctcggcg agcgtctgca gccggccttt gagctcggcg
     2521 agctgggcgt cgctctcgcg cacctcgcgc cgccgccggc tcatatgcac cgcgacgaac
     2581 accaccagca cgaagagggc aagcgccgcg aacccgagca gtccaagcag gaccagcacg
     2641 gggtcgagcg tgacgctccc cacctggata ggctctaggg ccatgggaag aacctatagc
     2701 ccgattcgtt ctttcagacc aaaacaggaa catattgacc gcccgtcccg ccgatcctat
     2761 gtcaccggct catatggcca tcctccccat catcaccatc ccggaccccc tgctgcgcaa
     2821 gaaggcggcg ccgatcgagc gggtcgagac cgcgctgctg aagctcgcgg aaaacatgct
     2881 ggcgaccatg tatgacgcgc caggcatcgg gcttgccgcg ccgcaagtcg gcattttgcg
     2941 ccggctgatc gtgatggatc cctcgcgtga cgaggaggcg aagcagcctc tggtcatggt
     3001 caacccggtg atcctcgagc ggggaagcga gatgcgcacg cacgaggagg gctgcctgtc
     3061 gatcccggac gtcaccgccg agatcgagcg gccggccgtg acccgcgtgg gcttcatcga
     3121 cagggaagga aagtcgcagg agcagacgct ggaaggcatc tggtcgacgc tggtgcagca
     3181 cgagatcgac catctgaacg gcgtcttgtt catcgactat ctgtcgcggc tcaagcgcga
     3241 catgatcgtg aagaaattca ccaagcagaa gcgcgccgac gcgctttagg ctgcgcgggc
     3301 ctccgcttcc cttcactgcg taaccggcct aagaggcacc catgatgcgc atcatcgtca
     3361 tgggcacgcc ggattttgcc gtgccggcgc ttggcgcggt gctcgctgcc ggccacgagg
     3421 tcgtcggcgt gtacactcag ccgccgcgcg cggccggtcg cgggctcgcc gcccggaaat
     3481 cgcgggtcca gctcttcgct gagagcaggg ggctgcccgt gcacacgccg gtaagcctca
     3541 gaagtgctga agagcaggcg cgcttcaagg cgctcaacgc cgacgccggc gtggtggtcg
     3601 cctatgggca gatgctgccg aaggcgatcc ttgaagcgcc gcactatggc tgcttcaata
     3661 ttcatgcctc gcttctcccg cgctggcggg gtgcggcgcc cattcagcgc gctcttctcg
     3721 ccggagacgc cgcgaccggt gtcgcggtta tgcgcatcga ggagaagctc gacaccggcg
     3781 ctgtctgcct tatggagcgc accgccatcg gccccgacga gacggcgggg gaactgcaca
     3841 atcgcctctc cgaactcggc gcggagctca tggtgcgggt cctgagcgat ctcgggaaag
     3901 gctcgctcgc ctgctggccg cagcacgaac agggcgtcac ctatgccgcc aagatcgagc
     3961 cgggagagac gcgcatcgac tggtctcgcc cggcccgcga cgtccacaat ctgatcaggg
     4021 gcctctcgcc ccatcccggc acctggttcg agatcgatct caacggcaag cgcgagcgcg
     4081 tcagggcctt gcgctcgacc ctcgcggaac gcgggggcga gccaggcacc gtgctcgacg
     4141 atcaactcac tattgcctgc ggcaaaggct cagtgcgcct gttgcagatg cagcgtgcgg
     4201 ggaagcggcc
//
LOCUS       DATNAE010000182         2470 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_4082991, whole genome shotgun sequence.
ACCESSION   DATNAE010000182 DATNAE010000000
VERSION     DATNAE010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4082991"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(141..1166)
                     /locus_tag="VN518_04045"
     CDS             complement(141..1166)
                     /locus_tag="VN518_04045"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013421023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HXE03778.1"
                     /translation="MGYKVAVIGATGNVGREMLNILAEREFPADEVFAVASRRSMGVE
                     VSFGDKRLTCRDLEQFDFRGVDFALMSAGSAVSKEWSPRIGAQGCVVIDNSSCWRYDP
                     DVPLIVPEVNADAVVGFSKKNIIANPNCSTAQLVVVLKPLHDHAKIKRVVVATYQSVS
                     GAGKEAMDELWDQTKGIFTTTPTEPSKFTKQIAFNVIPHIDVFMEDGSTKEEWKMMVE
                     TKKILDPKIKLTATCVRVPVFVGHSEAVNIEFEEPITAAEARKILREAPGVLVVDKRE
                     PGGYVTPVECVGDYATYVSRIREDQTVDNGLSLWIVSDNLRKGAALNTVQIAETIINR
                     NLLEKAA"
     gene            1315..>2470
                     /locus_tag="VN518_04050"
     CDS             1315..>2470
                     /locus_tag="VN518_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HXE03779.1"
                     /translation="MHRTISLTPIAAALLLLLAGCRDSAELPEQAAQGPNPSLPAPKE
                     RLIPTLNIAKATGWKQGETPDAAKGLAVGAYATGLDHPRWLYVLPNGDVLVAETNTPK
                     QDGAAGSGGVMDWIKGKVMIWAMNRAGAGVPSPNRIMLLRDADGDGVAETTSVFLKGL
                     NSPFGMALIGDKLYVADTDALLRFDYRDGGTEIAGEGTRVAELPAGPINYHWTKSLAA
                     SPDGSHLYVGIGSNSNAGENGLGWEKDRARIVAIDPATGEARPFATGLRNPVGLAFEP
                     QTGVLWAVANERDELGSDLVPDYLTSVRDGAFYGWPFSYWGQHVDDRVKPQNPEAVAA
                     AIPPDYALGNHVAPLGLAFAQGALQPEPLRTGAFIGEHGSWNRDPLGGYKV"
BASE COUNT          436 a          787 c          831 g          416 t
ORIGIN      
        1 ccttcatcca gaaacggatg aaggatgggc tgctcgcgct gcacgggctg catttcgatc
       61 tcggcagcgg cgagctgctg gcgctcgaac aaggaacggg acgcttcgcg gcgatcggcg
      121 agccttgagc ggcgcgcggc tcaggcggcc ttttccagca gattgcggtt gatgatggtc
      181 tcggcgatct gcacggtgtt gagcgcggca cccttgcgca gattgtcgga gacgatccag
      241 agcgacaggc cgttgtcgac ggtctgatcc tcgcggatgc ggctcacata ggtggcgtaa
      301 tcgccgacgc attcgaccgg cgtcacgtag ccgccgggct cgcgcttgtc caccaccagc
      361 acgcccggcg cctcgcgcag gatcttgcgc gcctcggcgg cggtgatcgg ctcctcgaac
      421 tcaatattga cggcctccga atggccaaca aacaccggca cgcgcacgca agtggcggtg
      481 agcttgatct tcggatcgag gatcttcttg gtctcgacca tcatcttcca ttcctccttg
      541 gtcgagccgt cttccatgaa gacgtcgata tgaggaatga cgttgaaggc gatctgcttg
      601 gtgaatttgc tgggctcggt gggcgtcgtg gtgaagatgc ccttggtctg atcccacaat
      661 tcgtccatcg cctctttgcc ggcaccggac acggactgat aggtcgccac caccacgcgc
      721 ttgatcttgg cgtggtcgtg cagcggcttc agcacgacga cgagctgcgc ggtcgagcaa
      781 ttcggattgg cgatgatgtt cttcttggag aagcccacca ccgcgtcggc attgacctcg
      841 ggcacgatca gcggcacgtc ggggtcgtaa cgccagcagg aggaattatc gatcaccacg
      901 cagccttgcg cgccgatgcg cgggctccat tcctttgaca cggccgagcc cgccgacatc
      961 agcgcgaagt cgacgccgcg gaaatcgaac tgctcgaggt cgcggcaggt gagccgctta
     1021 tcgccgaagg agacctcgac gcccatggag cgacgcgagg ccacggcgaa gacctcgtcg
     1081 gccgggaact cgcgctcggc caggatgttc agcatctcgc gcccgacatt gccggtggcg
     1141 ccgataacgg cgactttgta gcccattgat ctgctccatt gcgggtcgag aattgccccg
     1201 cgcctttgcg ccggatgtat gggaaggagt gcgcaaagta aagccgtgag ccccaggggc
     1261 ttgcaacccc aggcttcccg cgcgcgttgc tcaagctggt agaggactag cgacatgcat
     1321 cgaacgatct cactgacacc gattgcggcg gcgctgctgc ttctgctggc gggctgccgc
     1381 gacagcgccg aattgcccga gcaggcggcg caaggaccca atccgagcct gccggcgccg
     1441 aaagagaggc tgatcccgac gctcaacatc gccaaggcca cgggctggaa gcagggcgag
     1501 acacccgatg ccgccaaggg gctcgcggtc ggcgcctacg ccacagggct cgatcatccg
     1561 cgctggctct acgtgctgcc caacggcgat gtgctcgtcg ccgagaccaa tacgccgaag
     1621 caggacggtg cggccggaag cggcggcgtg atggactgga tcaagggaaa ggtgatgatc
     1681 tgggccatga atcgcgccgg cgccggcgtg ccgagcccga accgcatcat gctgctgcgc
     1741 gacgcggacg gcgatggcgt ggccgagacc acgagcgtgt tcctcaaggg gctgaactcg
     1801 cccttcggca tggcgctgat cggcgacaag ctctacgtcg ccgacaccga cgcgctgctc
     1861 cgattcgatt accgagacgg cgggaccgag atcgcgggag aggggaccag ggtcgccgag
     1921 ctgccggcgg gacccatcaa ctatcactgg acgaagagcc ttgccgcctc gcccgatgga
     1981 tcgcacctct atgtcggcat cggctccaat agcaatgcgg gcgagaacgg gctcggctgg
     2041 gaaaaggacc gcgcgcggat cgtggcgatc gatccggcga cgggcgaggc ccggcccttc
     2101 gccacgggct tgcgcaatcc ggtcgggctc gccttcgaac cgcaaaccgg cgtgctgtgg
     2161 gcggtggcga acgagcggga cgagctgggc tccgatctcg ttcccgacta tctcacctcg
     2221 gtgcgagacg gcgcgttcta cggctggccg ttcagctatt ggggccagca tgtcgacgat
     2281 cgggtgaagc cgcaaaatcc cgaagcggtg gcggcggcga tcccgcccga ctatgcgctc
     2341 ggcaatcacg tggcgccgct ggggctcgcc ttcgcccaag gggcgctcca gcccgagccg
     2401 ttgcggaccg gcgcgttcat cggcgagcac ggctcatgga accgcgatcc gctcggcggc
     2461 tacaaggtgg
//
LOCUS       DATNAE010000183         4867 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2891550, whole genome shotgun sequence.
ACCESSION   DATNAE010000183 DATNAE010000000
VERSION     DATNAE010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4867
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2891550"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="VN518_04055"
     CDS             complement(<1..344)
                     /locus_tag="VN518_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018262071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HXE03780.1"
                     /translation="MSKRNAVPSRVTVLAIAALLAGLPLRVGAQQNAEPGIGDNDLGG
                     IVRGPQGPEAGVWVIAETTELPTKMARIVVTDDQGRYVIPDLPKANYSVWVRGYGLVD
                     SPKQATAPGKIL"
     gene            complement(640..822)
                     /locus_tag="VN518_04060"
     CDS             complement(640..822)
                     /locus_tag="VN518_04060"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HXE03781.1"
                     /translation="MDADRSSESLTEMPKDKLTAELAYRLWQENGCVEGRALDYWLEA
                     ERTLRDTGSKDGTQER"
     gene            969..1490
                     /locus_tag="VN518_04065"
     CDS             969..1490
                     /locus_tag="VN518_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HXE03782.1"
                     /translation="MATSHTASHAKGKTRRTRRTGRRDPMAVQLLKKDHREVEEMFDE
                     FEQLDEDSEKLALFKKIALALKVHTQIEEEIFYPAERGDVEDDMLDEAQVEHDGAKKL
                     IAEIEKMRPGEELYDAKVKVLGEYIKHHVKEEEQPGGIFAQAKKGDQDLEAMGKEIKT
                     RKTELMRQMGGDA"
     gene            1573..1815
                     /locus_tag="VN518_04070"
     CDS             1573..1815
                     /locus_tag="VN518_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03783.1"
                     /translation="MKRGDELLALAQQQRLLLIQCLLEELEEGELTDALKAAAAATDQ
                     LIEILDRICREGADFRSAGKAPAGDGGRAKLARRRA"
     gene            complement(1892..2923)
                     /locus_tag="VN518_04075"
     CDS             complement(1892..2923)
                     /locus_tag="VN518_04075"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HXE03784.1"
                     /translation="MHPRLLRPALLLAALLLTCSTGRADPIEEFYRGRTVSFLVGEDV
                     GGGYDSYSRLFASHVGRHIPGNPVVVTQNMPGAAGLTAANHLYARAPKDGSVIGMIDQ
                     GLYLDQMLGKPGIGFDFAKFAWIGRLLRVTGVLVSWHTSPVKTAADLKSHALIVSATG
                     GSSRQNWLLLNGLTGAHLKIIGGYAGTANSALALERGEIEGMSFPWQVLATSQKRWLE
                     EGKVNVLLQTVLDKHPALADVPRMVDLAPDADAERILRLFSSPYSIGRAVMAPPGLPD
                     ATVAALRRAFDATLADRDFQAAAAKAGLEIDALPGEQLQALIAGGGSLPPALIERVRR
                     LVGVDSQAK"
     gene            complement(2940..3821)
                     /locus_tag="VN518_04080"
     CDS             complement(2940..3821)
                     /locus_tag="VN518_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03785.1"
                     /translation="MKIAYVDPNNELGADFRGNVKDGQILLTGEEGAPDNYRLNFSRQ
                     TGPFFSPRHKHNFDQIRIVLGGGPMNYGPKQWIQPGEIAYFPEGTPYGPQDYDSKRYG
                     MTLQFGGASGSGYISLPRMKEGMEALKAFGSFEKGIFTRSGDLPPGMKRNRDSYEAVW
                     EYINDRPLDYPKPRYNEPILIKPKHFDWQPDAEQPGLGTKHLGSFSERQIEIAMLKIE
                     AGSSARIAARAGVQIGIVVEGAGRIGKDDLRPHAAFSLEPGEGAMVSAATELALYLIG
                     LPIFARQTIRRPVQSAA"
     gene            4261..>4867
                     /locus_tag="VN518_04085"
     CDS             4261..>4867
                     /locus_tag="VN518_04085"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXE03786.1"
                     /translation="MSTSTEYSAVLPIFREMQQETIDGIPLSELVGGGDPPAVAGQMV
                     EVIKSAAGLNPTDHVLDIGCGCGRIAAALTQHLDPTSRYYGVDIVPGLVDFAQRHISS
                     HYPNFRFLTIDQKNSFYDTFRAKGIADISAVSEVCGERSIDLCIATSLFTHLTAKDTR
                     NYLSAIQSVLKPDGRAVVTFFVLDATTRALMQGGHPSFGFKH"
BASE COUNT          868 a         1516 c         1625 g          858 t
ORIGIN      
        1 tcgagaattt tgccgggcgc cgtcgcctgc ttgggcgaat cgacgagccc gtagccgcgc
       61 acccagacgc tgtaattggc tttcggaaga tcgggaatga catagcgccc ctgatcgtcg
      121 gtgacgacga tgcgtgccat cttggtcggc agctccgtcg tctcggcgat cacccacacg
      181 ccggcttccg gaccctgcgg cccgcggacg atgccgccga gatcattgtc accgatgccg
      241 ggctcggcat tctgctgcgc gccgacgcgc agcggcaagc cggcgagaag tgccgcgatg
      301 gcaaggacgg tgacgcgcga aggcacggcg tttctcttgc tcatctcacc gctcctttgt
      361 gttctttagg gtcgcacccg gcgtcgacgg gaaggcgagg cccggcaccc gtttcacagg
      421 acaggaaatc aaaggcgttg ctcttcgtcg cgcgctgatc gcgcgacagc atgccaaagc
      481 cggcatgccc gttttcgtgg ggattggtcc gcaaacgacc gcgaccgcca gtgtcgcgcc
      541 gcgaaaccgg caatacaaga tttattgcag tcatggctcc ggccgagagc tcctcggccg
      601 cggcggcacg actgggctcg ccggctggcg cccttgcgct caacgctcct gagtgccgtc
      661 ctttgaacct gtgtcgcgca gcgtgcgctc ggcctcgagc cagtaatcga gcgcccgtcc
      721 ctcgacgcag ccattttcct gccacaggcg ataggcgagc tccgccgtga gcttgtcctt
      781 gggcatttcg gttaggcttt cgctgctccg atccgcgtcc atccgcctct cctcgctcga
      841 agatgtgaga ggagaacgaa gcgtcgcgca aaggttcgcg cccgtgtgac atcgctatgg
      901 cctctccagg aacatccgct gcgcagcggg tgttgatggg agagcttcgg agaattagga
      961 gaagtcttat ggcgaccagt cacaccgcat cgcacgccaa gggaaagacg cgccgcaccc
     1021 gccgcaccgg ccggcgcgat ccgatggcgg tgcagctctt gaagaaggat caccgcgagg
     1081 tcgaagagat gttcgacgag ttcgagcagc tcgacgagga cagcgagaag ctcgcgctgt
     1141 tcaagaagat cgccctcgct ctcaaggtgc acacccagat cgaggaggag atcttctatc
     1201 cggccgagcg cggcgacgtc gaggacgaca tgctggacga ggcgcaggtc gagcatgacg
     1261 gcgccaagaa gctgatcgcc gagatcgaga agatgcggcc cggcgaggag ctctatgacg
     1321 cgaaggtcaa ggtgctcggc gagtacatca agcaccatgt gaaggaagaa gagcagcccg
     1381 gcggcatctt cgcccaggcg aagaagggcg accaggacct cgaggcgatg ggcaaggaga
     1441 tcaagacccg caagaccgag ctgatgcgcc agatgggcgg cgacgcctga tcaggctggc
     1501 ttgtcgcggc ggcgcgcgcc cggggattcc cgattcggag caactgttgg ggccgttgat
     1561 tggaaagaag ccatgaagcg cggtgacgag ctgctggcgc tcgcccagca gcagcgacta
     1621 ttgctgattc agtgcctgct cgaagagctc gaggaaggcg agctgaccga tgcgctcaag
     1681 gcggcggccg ccgcgaccga ccagctgatc gagatattgg atcggatctg ccgcgagggt
     1741 gccgattttc gcagcgccgg caaggcgccg gcgggcgatg gcggacgcgc gaaactggcg
     1801 cgccgccgcg cttagccgag acgaagctcg ggcaggagaa gccgtcctgc tgcgacagca
     1861 ggcggcgctc cctgtcgtct ccggcgtcgg ctcacttggc ctggctgtcg actccgacca
     1921 ggcggcgcac ccgctcgatg agggccggcg gcaggctgcc gccgccggcg atgagcgcct
     1981 gcagctgctc gccgggcagc gcatcgatct cgagccccgc cttggcggcg gcggcctgga
     2041 agtcgcgatc ggcgagcgtc gcgtcgaagg cgcggcgcag ggccgcgacg gtcgcgtcgg
     2101 gcaggccggg cggcgccatc acggcgcgac cgatcgagta gggcgaggaa aagagacgca
     2161 ggatgcgctc ggcatccgcg tcgggcgcga gatcgaccat gcgcggcacg tcggccagcg
     2221 ccggatgctt gtcgagcacg gtctgcagca gcacattgac cttgccctcc tcgagccagc
     2281 gcttctggct cgtcgccagc acctgccagg gaaagctcat gccctcgatc tcgccgcgct
     2341 ccagcgcgag ggcgctgttc gccgtgccgg catagccgcc gatgatcttg agatgggcgc
     2401 ccgtgagacc gttgagcagc agccaattct gccgcgacga gccgccggtc gccgagacga
     2461 tcagcgcgtg gcttttgaga tcggcggccg tcttgaccgg agaggtgtgc caggagacga
     2521 gcacgcccgt gacccgcagc aggcgcccga tccaggcgaa tttcgcgaag tcgaagccga
     2581 tgcccggctt gcccagcatc tgatcgagat agagtccctg gtcgatcatg ccgatgacgg
     2641 agccgtcctt gggcgcccgc gcgtaaagat ggttggccgc ggtgaggccg gccgcgcccg
     2701 gcatgttctg cgtgacgacc acgggattgc cgggaatgtg ccggccgaca tggctcgcga
     2761 acaggcggct gtagctgtca tagccgccgc cgacatcctc gccgaccagg aacgagacgg
     2821 tacggccgcg gtagaattcc tcgatcggat cggcgcggcc ggtgctgcag gtcagcagca
     2881 gcgccgcgag cagcaatgca ggccgaagaa gccgcgggtg catgcgcgga tccgccggct
     2941 caggccgccg actgcacggg gcgtcgtatc gtctggcgcg cgaagatcgg caggccgatg
     3001 agatagagcg cgagctccgt cgcggcgctc accatggcgc cctcgccggg ctcgagcgag
     3061 aaggcggcat gcgggcggag atcgtccttg ccgatcctgc ccgccccctc gacgacgatg
     3121 ccgatctgca cgccggcccg cgccgcgatg cgggcgctcg atccggcctc gatcttcagc
     3181 atcgcgatct cgatctggcg ctcggagaag ctgccgaggt gcttggtgcc gaggcccggc
     3241 tgctcggcat ccggctgcca gtcgaaatgc ttcggcttga tcagaatcgg ctcgttgtag
     3301 cgcggcttgg gatagtcgag cggccggtcg ttgatgtatt cccagaccgc ctcgtagctg
     3361 tcgcgattgc gcttcatgcc cggtggcaga tcgccgctgc gggtgaagat gcccttctcg
     3421 aagctgccga aggctttgag ggcctccatg ccctctttca tgcgcggcag gctgatatag
     3481 ccgctgccgc ttgccccgcc gaactgcagc gtcatgccat agcgcttgct gtcgtagtcc
     3541 tgcggcccgt aaggcgtgcc ttccggaaaa taggcgatct cgccgggctg gatccattgc
     3601 ttgggcccgt aattcatcgg cccgccgccc agcacgatac ggatctggtc gaaattgtgc
     3661 ttgtggcgcg ggctgaagaa ggggccggtc tggcggctga aattcagccg gtaattgtcg
     3721 ggcgcgccct cctcgccggt gagcaggatc tgcccgtcct tcacattgcc gcggaagtcg
     3781 gcgccgagct cattgttcgg atcgacatag gcgatcttca tggcgtgtct cccaacgagc
     3841 ggcgggcggg cgggccgctg ccattttcgg tatttgcgcc cagtctacag gtccgcccgc
     3901 agcaggcgta gagccaagtg gactggccct ggaaacccgc gtaccgagca aaaccatact
     3961 aaggctcaag ctttccgtgc ggcggcggtt tctttcctgc taactatctg ggaaactggc
     4021 caaggctgaa cggcgcaagc ggacaagacg gaatggcttg gaagcgcgca gcggccgatt
     4081 tgatggagcc cacagaatag aagagaagga tgccggcggg gctgtcgccg gactccgtag
     4141 catctgctat tgagcagcct gaaattctct ttcggactcc atgaaactgt ctctgttcga
     4201 tggtcggtgc acctcgacaa ggggtaagag ggagcattga aggaaaagga cgcgatcagc
     4261 atgtcgacct ccaccgaata ttccgccgtg cttccgatat ttcgggaaat gcagcaagag
     4321 accatcgacg gcataccgct ctcggagctg gtggggggcg gcgatccgcc ggccgttgcc
     4381 ggtcagatgg tcgaggtgat caagtccgcc gccggactga atccgacaga ccatgtcctc
     4441 gatataggct gcggttgcgg tcggattgcc gctgctctga cgcaacatct cgatccaacg
     4501 tcacgctatt atggtgtcga cattgtaccg ggacttgtcg attttgcgca gcgccacatc
     4561 tcgtctcact atccaaattt tcggttcctg acgatcgacc agaaaaattc cttctatgat
     4621 acctttcgag cgaagggcat cgccgacatt tcggcggtga gcgaggtgtg cggcgagcgg
     4681 tccatcgatc tttgcatcgc aacatctctg tttacccatt tgaccgccaa ggatacgcgg
     4741 aactatctgt cagccataca gagcgtgctg aagcccgacg ggcgggcggt cgtgacgttt
     4801 tttgtcctcg atgccacgac ccgggccctt atgcagggtg gccatccttc cttcgggttt
     4861 aagcatg
//
LOCUS       DATNAE010000184         2373 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_782004, whole genome shotgun sequence.
ACCESSION   DATNAE010000184 DATNAE010000000
VERSION     DATNAE010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_782004"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="VN518_04090"
     CDS             <1..432
                     /locus_tag="VN518_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXE03787.1"
                     /translation="FWPYAARLEAAGFKQLIGIEDVVSELGAKGEVAMVGYVFSESWA
                     NENLKAVGGFLRAAAKADELLATSNEAWEAVRPLMRAEDQATFEALRRRYREGIPERS
                     IAENESDAKVLYQFLRELGGEKLVGPGAELAPGTFWKGGKS"
     gene            489..1226
                     /locus_tag="VN518_04095"
     CDS             489..1226
                     /locus_tag="VN518_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXE03788.1"
                     /translation="MVSVLSVAGFLLVWFIAAEIAQSRLLPGPVEVLSYVVDEALHGD
                     LLAELGITLWRVTASFVVAMAIGSVLGLAMGESPALNRALDPWLIVLLNLPALVIIIL
                     AYVWFGLNEAAAIGAVALNKIPNAIVTMREGARALDPGLDEMARAYRLSYWSKLRNVT
                     LPQLQPYFAAASRSGISLIWKIVLVVELLGRPNGVGFELHLNFQLFNVTAILGYTVAF
                     VTVMLAIEYLLVQPLERRTTRWRNKPV"
     gene            1186..1935
                     /locus_tag="VN518_04100"
     CDS             1186..1935
                     /locus_tag="VN518_04100"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXE03789.1"
                     /translation="MNDVRPAGATSRFEIRIEEKTHVGADGTRIPAIRDLVLEVQPQS
                     MTVLMGPSGCGKTTLLRIVAGLDDRFTGKVHVPDNARIGVMFQEPRLLPWRTVRQNIQ
                     IVAEDMSNAELERLASAVGIADMLPRYPQELSLGLARRVALARAFAPHPDLLLLDEPF
                     VSLDERTADRLRHLLLQVWSERPTTAILVTHNPREAILLADQLVLLAPRPTHVVAVVP
                     IPIPQGERHASVVEEIYADLLKRHPANFATG"
     gene            complement(1939..>2373)
                     /locus_tag="VN518_04105"
     CDS             complement(1939..>2373)
                     /locus_tag="VN518_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXE03790.1"
                     /translation="FLLVAYFFGVELPPWGYLAALPALILTGMMFGALGMLVSSTIKQ
                     LENFAGIMNFVIFPMFFASSALYPLWKMREGSLWLYYICIYNPFTYGVELIRFAFYLE
                     VNWQALAVVLGCLLVFVGLSVWAYDPSVGIQQRKAAAAAAAA"
BASE COUNT          411 a          772 c          769 g          421 t
ORIGIN      
        1 ttctggcctt atgccgcaag gctcgaggcg gcaggcttca agcagctgat cggcatcgag
       61 gacgtggtga gcgagctcgg cgccaagggc gaggtggcca tggtcggcta tgtgttcagc
      121 gagagctggg ccaacgagaa cctgaaggcc gttggcggct tcttgcgcgc ggcggccaag
      181 gccgacgagc tgctcgcgac gtcgaatgag gcgtgggagg cggtcaggcc cctgatgcgg
      241 gcggaagacc aggcaacgtt tgaggcgctc cggcggcgct accgcgaggg cattccggaa
      301 cggtcgatcg ccgaaaacga gtcagacgcc aaggtgctct atcaattctt gcgcgagctt
      361 ggcggggaga agcttgtcgg gcccggcgcc gagctcgccc ccggcacctt ctggaaaggc
      421 ggcaagtctt gagcgtaagc gcggcccctc ccgacacttt gagcgggctc cgcggccttc
      481 gcgggccgat cgtgtccgtg ctctcggtcg ccggcttcct gctcgtgtgg ttcatcgccg
      541 ccgagatcgc gcagagccgg ctgttgcccg gcccggtgga agtgctgagc tatgtcgtcg
      601 acgaggcgct gcacggcgat ctccttgccg agctcggcat cacgctctgg cgggtgacgg
      661 cgtctttcgt ggtggcgatg gccatcggca gcgtgctcgg cctcgccatg ggcgagtcgc
      721 cggcgctcaa ccgggcgctc gatccctggc tgatcgtgct tttgaacctg cccgccctcg
      781 tcatcatcat cctcgcctat gtctggttcg ggctgaacga ggccgcggca ataggtgccg
      841 tcgccctgaa caagatcccg aacgccatcg tcaccatgcg cgagggcgca agggcgcttg
      901 accccgggct cgacgagatg gcgcgggcct atcgcctgtc gtattggagc aagctccgca
      961 atgtcaccct gccgcagctt cagccctatt tcgccgccgc ctcccgctca ggtatctcat
     1021 tgatctggaa gattgttttg gtggtggagc tgctcggcag gccgaatggc gtaggcttcg
     1081 agctccatct aaacttccaa ctttttaacg tgacggccat cctcggctat accgttgcct
     1141 tcgtcaccgt catgctcgca atcgaatatt tgctggtgca gccccttgaa cgacgtacga
     1201 cccgctggcg caacaagccg gtttgagatc cgcatcgagg agaagaccca tgtcggggcg
     1261 gacgggacgc ggatccccgc catccgcgac ctggtgctcg aggtgcagcc gcaaagcatg
     1321 acggtgctga tgggcccgtc gggttgcggc aagaccacgc tgttgcgcat cgtcgccggc
     1381 ctcgacgatc gcttcaccgg caaggtgcac gtgcctgaca acgcgcgcat cggcgtcatg
     1441 ttccaggagc cgcggctgct tccctggcgc acggttcggc agaatatcca gatcgtcgcc
     1501 gaggacatgt cgaacgccga gctcgagcgt ctcgcctctg cggtcggcat cgccgacatg
     1561 ctgcctcgct acccgcagga gctgtcgctt gggcttgcgc gccgcgtggc cttggcccgc
     1621 gccttcgcgc cgcatcccga cctgttgctg ctcgacgagc ccttcgtctc actcgatgag
     1681 cgcaccgccg accgcttgcg ccatcttctg ctccaagttt ggtcggagcg cccgacgacg
     1741 gcgatcctcg tcacccacaa tccgagagaa gcgatcctgc tcgccgatca gctcgtactg
     1801 ctcgcgcccc ggccgaccca tgtggtcgcg gtcgtgccga tccccatacc gcaaggcgag
     1861 cgacacgcca gcgttgtcga agagatctac gccgatctcc tcaagcgcca tccggccaat
     1921 ttcgccaccg gctgatcctc aagccgctgc tgcggcggcg gcggccttgc gctgttggat
     1981 gccgacggac gggtcgtaag cccacaccga cagcccgacg aagacgagca ggcacccgag
     2041 caccacggcg agcgcctgcc agttcacctc gagatagaag gcgaagcgga tcagctcgac
     2101 accataggtg aaggggttgt agatgcaaat gtaatagagc cacaggctgc cctcgcgcat
     2161 cttccacagc ggatagagcg ccgacgacgc gaagaacatc gggaagatca cgaagttcat
     2221 gatgccggcg aagttctcga gctgcttgat ggtcgaggac acgagcatgc cgagcgcgcc
     2281 gaacatcatg ccggtgagaa tgagcgccgg gagtgccgcg aggtagcccc aagggggcag
     2341 ctcgacgccg aagaaataag ccacgagcag gaa
//
LOCUS       DATNAE010000185         2208 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_1687555, whole genome shotgun sequence.
ACCESSION   DATNAE010000185 DATNAE010000000
VERSION     DATNAE010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1687555"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="VN518_04110"
     CDS             complement(<1..440)
                     /locus_tag="VN518_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HXE03791.1"
                     /translation="MSGAQSAGHLKPGMELPALVLPATDGRQVDLAALAGKTLLIVYP
                     WTGRPGHSNPPDWDAIPGAHGSTPELEGFRDRAAEFAALGLRLIGLSRQTTEYQRELV
                     ARLGLLFPILSDADGRFTAALRLPSFSTGGEIYLRRLTLLIEEGR"
     gene            complement(562..1032)
                     /gene="smpB"
                     /locus_tag="VN518_04115"
     CDS             complement(562..1032)
                     /gene="smpB"
                     /locus_tag="VN518_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HXE03792.1"
                     /translation="MAPKRESGKLVAENRRARFNYAIEDKLEAGIALTGSEVKSLRAG
                     KANIADSYASSEGGELYLINAHIAEYVQAGRNGHAPLRPRKLLLHRKEIGKLAGAIQR
                     EGMTVVPLKMYFNARGIAKVELGLAKGKKLHDKRETEKKRDWERQKGRLMREKG"
     gene            complement(1050..1934)
                     /gene="dapA"
                     /locus_tag="VN518_04120"
     CDS             complement(1050..1934)
                     /gene="dapA"
                     /locus_tag="VN518_04120"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HXE03793.1"
                     /translation="MPPFQGSITALITPFSNGKVDAEAFRKLVKWQIDQGTHGLVPCG
                     TTGESPTLSHDEHREVVELCIEAASGRVPVIAGTGSNSTAEAVELTLHAKSAGADGVL
                     VVTPYYNKPTQEGLYLHYKAINDCTDIPILIYNIPGRSVVDMTVDTMARLFKLKNIVG
                     VKDATASMARVSQQRAALGKDFVQLSGEDATALGFMAHGGQGCISVTANVAPALCSEF
                     QLACLGGNFKRALELQDRLMPLHDALFVESNPGPVKYAASKLGLCNGETRLPLAPMAP
                     ASKQKVDEAIAAVGLVLA"
BASE COUNT          367 a          693 c          718 g          430 t
ORIGIN      
        1 cgtccctcct cgatcagcag cgtaagacgg cgcagataga tctcgccgcc agtggagaag
       61 ctcggcagcc ggagcgcggc agtgaagcgc ccatcggcat cgctcaggat cggaaacagc
      121 agcccaagcc gcgcgacgag ctcgcgctga tattccgtag tctgacggct taatccgatc
      181 agtcgcaggc ccagtgcggc aaactcggcc gcgcggtcgc gaaagccttc gagctcggga
      241 gtcgagccgt gtgcgccagg gatcgcatcc cagtcgggcg ggttggaatg gcctgggcgg
      301 ccggtccacg gatagacgat aaggagcgtt ttgccggcaa gcgccgcgag gtcgacctgt
      361 ctgccgtcgg tcgcgggcag gacgagcgcc ggtagctcca tcccaggctt gaggtggccc
      421 gcactctgcg ctccgctcat cgcctgctct ccgtcattcc ggcgaaagcc ggaatccacg
      481 gttgagacac gaggagcaag cggctctgga taccggcttt cgccggtatg acgaagtccg
      541 ctggaaatct ttccggttgc ctcaaccctt ctcgcgcatc aggcggccct tctggcgctc
      601 ccaatcccgc ttcttctccg tctcgcgctt gtcatgcagc ttcttgccct tggccaggcc
      661 gagctcgacc ttggcgatgc cgcgcgcgtt gaaatacatc ttgagcggaa cgaccgtcat
      721 gccctcgcgc tgaatggcgc cggcgagctt gccgatctcc ttgcgatgca gcaggagctt
      781 gcgcggacgc aggggtgcat gaccgttgcg gccggcctgc acatattcgg cgatgtgggc
      841 gttgatcagg taaagctcgc cgccctcgct cgatgcgtag gagtcggcga tattggcctt
      901 gcccgcgcgc agcgacttga cctcggaacc ggtcagcgcg atgcctgcct cgagcttgtc
      961 ctcgatcgcg tagttgaagc gcgccctccg attctcggcg acgagcttgc cgctctcgcg
     1021 cttcggcgcc atcacgtcac ctatagggtc taggcaagga cgaggccgac cgcggcgatc
     1081 gcctcgtcga ccttctgttt ggaggccggc gccatcggcg ccagcggaag ccgcgtctcg
     1141 ccgttgcaaa gcccgagctt gctcgcggca tatttcaccg ggccgggatt ggattccacg
     1201 aacagcgcgt catgcagcgg catcagccgg tcctgaagct cgagcgcgcg cttgaaattg
     1261 ccgccgaggc aggcgagctg gaactccgag cagagcgcgg gcgcgacgtt ggcggtgacg
     1321 gagatgcagc cctgcccgcc atgcgccatg aagccgagcg cggtggcatc ctcgccggaa
     1381 agctgcacga agtccttgcc gagcgccgca cgctgctggc tcacccgcgc catgctggcg
     1441 gtggcgtcct tgacgccgac gatgttcttc agtttgaaca atcgcgccat ggtgtcgacg
     1501 gtcatgtcga cgacgctgcg gcccgggatg ttgtagatga ggatcgggat gtcggtgcag
     1561 tcgttgatcg ccttgtagtg cagatagagc ccttcctgcg tcggcttgtt gtaatagggc
     1621 gtcaccacga gcacgccgtc ggcgccggcg ctcttggcgt gcagggtcag ctccaccgcc
     1681 tcggcggtcg agttcgatcc ggtcccggcg atcaccggga cgcggccgga cgccgcctcg
     1741 atgcaaagct ccaccacctc tctgtgctcg tcatgggaga gcgtcggcga ttcgccggtg
     1801 gtgccgcagg gcaccagccc gtgcgtgccc tggtcgatct gccacttgac gagcttgcga
     1861 aacgcctcgg catcgacctt gccgttgctg aagggcgtga tcagcgccgt gatcgagcct
     1921 tgaaacggcg gcatgggcac gagaggaact cctgtggcgt cgcgcttggt aaattcgggt
     1981 cggcagggac cgcggacaat aggggttgaa gattgaggcg gcaagccgca gaaacccttg
     2041 gagatgttgg cttgacatga gccctgaccg gcacacgagg ctcgctatcg catcagttaa
     2101 aagctatttt attcattggc ttatcatgct ttgttgagaa tacctgtgat gcctgggcgg
     2161 ccgttccagt catttttctg ccggattctt tcagacattg ttgaaatc
//
LOCUS       DATNAE010000186         2334 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_783346, whole genome shotgun sequence.
ACCESSION   DATNAE010000186 DATNAE010000000
VERSION     DATNAE010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783346"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VN518_04125"
     CDS             <1..375
                     /locus_tag="VN518_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF815 domain-containing protein"
                     /protein_id="HXE03794.1"
                     /translation="KAVLEGGIEGRPAKVLLYATSNRRHLMPREMVDNERSTAINPAE
                     AVEEKVSLSDRFGLWIGFHNCSQDDYLAMVRGYAKHYGLSVPQSELDRDALEWARGRG
                     SRSGRVAWQFIQDLAGRLDKRL"
     gene            complement(376..1737)
                     /locus_tag="VN518_04130"
     CDS             complement(376..1737)
                     /locus_tag="VN518_04130"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HXE03795.1"
                     /translation="MSRFDFPSFNLTDNNSGEGNADLATTSSIPVPPESVYSSGGSYQ
                     GQVARTTLPPPDYTPKPASYSPVPPDRSRIARATYSGGIAPHGESAQRDDKPIAGLHI
                     KVGKGGTLSVLSRRYGVPVETIMGANDLSDGRLHIGQELVIPGAKAQRQTAEAGAKAQ
                     PTAEAQPPAPTGETYKVQKGDTPYSIATKLGVSQKALMERNKLRANSLQIGQVLSVPA
                     KTAQNVAANAPKETKPASATEPQVHVVKTTTITAPGAASSTQGSPGQGTSLAEEEDNQ
                     ASGAKAKGKPDDKAKPAGEAAASPEVIGEGTAPVASNDQLPTPDPMSGNSFRWPVKGR
                     VISEFGTRPDGGHNDGIDLAVPAGTSVLAAENGVVAYAGNELKGYGNLVLIRHANNWV
                     SAYAHNEEILVKRGDKVRRGQVIAKAGATGSVSQPQVHFELRKGSRPVDPTKFMSSEA
                     ASN"
     gene            complement(1954..>2334)
                     /locus_tag="VN518_04135"
     CDS             complement(1954..>2334)
                     /locus_tag="VN518_04135"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HXE03796.1"
                     /translation="TVERWRELQKAADRRFAKLGITNVTTIIGDGWLGWPPQAPFDRI
                     IVTAAAPEAPSALIDQLKIGGRMIIPLGETRDTQMLIQIDKSEAGVSGIELLPVRFVP
                     LVHGRAGLAARQGKSGGTTKPGRA"
BASE COUNT          370 a          752 c          783 g          429 t
ORIGIN      
        1 aaggcggtcc tcgaaggcgg catcgaagga cgcccggcca aggtgttgtt gtatgcgacc
       61 tcgaaccgac ggcatctgat gccgcgcgag atggtcgata acgaacgctc gactgccatc
      121 aaccctgccg aggcggtaga ggagaaggtc tcgctgtcag accgcttcgg gctatggatc
      181 ggctttcaca attgcagcca agacgactat ctcgcgatgg tgcgcggcta tgccaagcat
      241 tatggcctgt cggtcccaca atcggagctc gacagagacg cgctcgaatg ggcgcgtggc
      301 cgcggcagcc gatcggggcg cgtcgcctgg cagttcatcc aggacctcgc cggccggctc
      361 gacaagcggc tctagctagt tgctcgccgc ctcgctgctc atgaacttag tgggatcgac
      421 cgggcgcgag cctttgcgca gctcgaaatg cacctgaggc tggctgaccg atccagtggc
      481 gcccgccttg gcgatgacct gcccgcgccg caccttgtcg ccgcgcttga ccaggatctc
      541 ctcgttgtgc gcgtaggccg acacccaatt gttggcgtgg cggatgagaa cgagattgcc
      601 gtaacccttg agttcgttcc cggcataggc gacgacgccg ttctccgcgg cgagcaccga
      661 ggttcccgcg gggaccgcga gatcgatgcc gtcgttatgg ccgccgtcgg gccgggtacc
      721 gaactcggag atgacgcgac ccttcaccgg ccagcggaag ctattaccgg acatggggtc
      781 cggcgtcgga agctggtcgt tggaggcgac aggcgcagtc ccttcgccga tcacctcggg
      841 agaggccgcc gcctcgccgg cgggcttcgc cttgtcatca ggcttgccct tggccttggc
      901 tccgctcgcc tgattgtcct cctcctcggc gagcgaagtg ccctgaccgg ggctgccttg
      961 cgttgaggaa gctgcaccgg gcgccgtgat ggtcgtggtc ttgaccacat ggacttgagg
     1021 ctcggtcgcg ctcgcgggct ttgtctcctt gggcgcgttg gcggcgacat tttgcgccgt
     1081 cttggccgga accgacagca cctggccgat ttgcaggcta ttggcgcgga gcttgttccg
     1141 ctccatcagg gccttctggc tgacaccaag cttggtggcg atcgagtagg gcgtatcgcc
     1201 cttctgaacc ttgtaggtct cgccggtcgg cgcgggtggc tgagcctctg cggtcggctg
     1261 agccttggca cctgcctccg ccgtctgtcg ctgagccttg gcgcccggaa tgacgagctc
     1321 ttggccgata tgcagacgcc cgtcgctcaa atcgttagcg cccatgatgg tctcgaccgg
     1381 cacgccgtag cgccgcgaca gcaccgacag agtgccgccc ttgccgacct tgatgtgcag
     1441 accggcgatc ggcttgtcgt cacgctgcgc gctctcgcca tgcggggcga tgccgccgga
     1501 atacgtcgcg cgcgcgatgc gcgagcggtc cggggggacc ggagagtagg acgcgggctt
     1561 gggcgtgtag tcggggggcg gcaacgtcgt ccgggcgacc tgtccttgat agctccctcc
     1621 ggaactatag accgactctg gcggcacggg gattgacgag gtcgtggcga gatcggcatt
     1681 accctcgcct gaattgttgt cggtgaggtt gaacgaggga aagtcgaagc gcgacatgcc
     1741 cgagctgcag ccggaaacgc agagtgcaac gccggtcgcg gcgacaattc tccaagcgcc
     1801 gcggcccttg cttgaccaaa acgccagccg cattccctca aacccctcaa cgcaacacac
     1861 accgcacccc gctccggagt aaagcgcgcg agcagttacc aagctgttaa gactctttgt
     1921 ggagccgccc aaaacgactc gcgtgccagg agctcaggcg cggccgggct tggtggtccc
     1981 gccactcttc ccctggcgtg ccgcgagccc tgcccggccg tgaaccagcg gcacgaagcg
     2041 cacgggaaga agctctatcc cgcttacgcc ggcttcgctc ttgtcgatct gaatgagcat
     2101 ctgggtgtcg cgcgtctcgc ctaagggaat gatcatgcgg ccgccgatct tgagctggtc
     2161 gatcagggcg gacggagcct cgggcgccgc cgccgtgacg atgatgcggt cgaacggcgc
     2221 ctgcggcggc cagccgagcc agccatcgcc gatgatggtg gtgacgttgg tgatgccgag
     2281 tttggcgaag cgccggtcgg ccgctttctg cagctcgcgc cagcgctcga cggt
//
LOCUS       DATNAE010000187         2908 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_1990425, whole genome shotgun sequence.
ACCESSION   DATNAE010000187 DATNAE010000000
VERSION     DATNAE010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1990425"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..1190
                     /locus_tag="VN518_04140"
                     /pseudo
     CDS             1..1190
                     /locus_tag="VN518_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083813.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            1256..2200
                     /locus_tag="VN518_04145"
     CDS             1256..2200
                     /locus_tag="VN518_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXE03797.1"
                     /translation="MGAYLIKRFLLMIVTLIGISIIIFALLRLVPGNIADILFDSAGM
                     IDPADHAQIEHDLGLDKPIPEQYVLWIGGLFKGDLGYSYVSEKPALEEILPRLPITGK
                     LAALALLFSVIIGVPLGVISAVKQNTGVDYLLRVLSLSGLAMPSFWLGLLILMVFVAR
                     FGTIPIYSDPKSFGEALITFGVPAAAVGFRSSALIMRLTRSSMLEVLRQDYVRTARSK
                     GASEPSVNYHHALRNAMLPVVTVIGIEAAFLMGGLIVTETVFNIPGVARFLVEAIRWR
                     DYPIVQNLVMFTAVIVVSINFLVDILYAALDPRIKLAE"
     gene            2233..>2908
                     /locus_tag="VN518_04150"
     CDS             2233..>2908
                     /locus_tag="VN518_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXE03798.1"
                     /translation="MHSIDYDAELAKAGARARGFWSIFALFLRRYPLGAIGAAIMIVF
                     VLAAIFADFITLYDPLSTNAKLSLAAPGEGHWLGDDFMGRDVYSRIVYGARISLAVGL
                     GSTALGCAIGVFLGLASGYLGGWVDLAVQRLVDILQGLPLLVLALVMAASLGPSLRNT
                     IIAIAVPLIPYAARVIRANTLALRELPFVEAARATGMSELRIALRHVLPNTLAPLIVL
                     ATAQLGA"
BASE COUNT          500 a          994 c          860 g          554 t
ORIGIN      
        1 cgcgcaagaa ctggtacgag aacgtgaccg aggtcacgac caacggcgac ttcgaggcga
       61 cgttcaatct gaagcggccg cagccggcgc tgctggcgct tctggcctcg ggctattcgg
      121 cggtctatcc ctgccatgtc tcgccggcgc agatgcgcac gcatcccatc ggcaccggcc
      181 ccttcaaatt cgtcgagttc aagctcaacg agtcgatcaa gctcgtgcgc aatccggact
      241 attggaagaa ggggaagccc tatctcgacg gcatcgaatt caccatcatc cccgatcgct
      301 cgacggcgat cctcgccttc atcgccggca agttcgacat gaccttcccg accgaggtga
      361 cgatcccgct cctgaaggac gtgaaatcgc aggcgcccaa ggcgcaatgc gtggtggcgc
      421 cgctcaacgt caacatcaat ctgctgatca atcgcgacaa accgcccttc gacaatgccg
      481 atatccgccg cgccatggcg cttgccctcg accgcaaggc cttcgtcgac atcctcttcg
      541 agggccaggc cgatatcggt gggacgatgc tgccaccgcc cgaggggagg tggggcctgc
      601 cgccggatat gctcaagacg atcccgggct atgggcccga cgtgaagaaa aatcgcgagg
      661 atgcgcgcgc catcatggcc aagctcggct atggcccgga caagcatctg agcgtcaagg
      721 tctcgacgcg caacattccc atctaccgcg accccgccgt cattctcatt gaccagctta
      781 aggacatcta tatcgatggc tagctcgagc ccgtcgatac cggcgtgtgg ttcgccaaga
      841 tggcgcgcaa ggattacagc atcgggctca acctcaccgg caatgcggtc gacgatcccg
      901 accaggcctt ctacgagaac tacgcctgca actccgagcg caactacacg caatactgca
      961 acaaggagct ggagaaggat ttcgaccagc aatcgatgga gaccgacctc gccaagcggc
     1021 agaagctcgt ctgggagatc gacaagaagc tccaggagga tgtggcgcgg ccgatcatcc
     1081 tgcatatgcg ccaaggcacg tgctggcagc cttacgtgaa gggcatcacc gtcatggtga
     1141 acagctccta taacggctat cgctacgagg atgcgtggct cgacaaatag ctgatggcgt
     1201 cgggtcggtc gccgcgaaag cctgctgcgc tttgccaggg tgagggaggc gcgccgtggg
     1261 agcctatctg atcaagcgct ttctcctgat gatcgtgacg ctcatcggca tctcgatcat
     1321 catcttcgcg cttttgcgcc tggtgccggg caacatcgcc gatatcctgt tcgacagcgc
     1381 cggcatgatc gatcccgccg accacgcgca gatcgagcat gatctcggcc tggacaagcc
     1441 gatccccgag caatacgtcc tgtggatcgg cggtcttttc aaaggcgatt tgggatattc
     1501 ctacgtctcg gagaagccgg cgctcgagga gatcctgccg cggctgccca ttaccggcaa
     1561 gctcgccgcg ctggcgctgc tcttctccgt catcatcggc gtgccgctcg gcgtcatcag
     1621 cgcggtgaag cagaacaccg gtgtcgacta tctcctgcgc gtcttaagtc tcagcggcct
     1681 tgccatgccc tccttctggc tgggcctgct catcctcatg gtgttcgttg cgcgcttcgg
     1741 cacgatcccc atctacagcg accccaagag cttcggcgag gcgctcatca ccttcggcgt
     1801 gcccgcggcg gccgtcggtt tccgcagctc cgccctcatt atgcgcctga cgcgctcctc
     1861 gatgctcgaa gtgctgcgcc aggattacgt gcgcaccgcg cgctccaagg gtgcctccga
     1921 gccctccgtc aattatcacc acgcgctgcg caacgccatg ctgccggtgg tgacggtcat
     1981 cggcatcgag gcggcttttc tcatgggcgg cctcatcgtc accgagacgg tgttcaacat
     2041 ccccggcgtc gcgcgcttcc tcgtcgaggc gatccgctgg cgcgattatc ccatcgtgca
     2101 gaacctggtg atgttcaccg cggtgatcgt cgtcagcatc aatttcctgg tcgacatcct
     2161 ctatgcggcg ctcgatcccc gcatcaagct cgccgaatag gccggcgatg gcgccgcgac
     2221 ggagtgcgtc ccatgcatag catcgactac gatgccgaac tcgccaaggc gggcgccaga
     2281 gcgcgcggct tctggagcat tttcgccctt ttcctgcgcc gctatcccct gggcgcgatc
     2341 ggcgcggcga tcatgatcgt cttcgtgctg gccgccatct tcgccgactt catcaccctc
     2401 tacgatccct tgagcaccaa cgccaagctg tcgctcgccg cgcccggcga gggacactgg
     2461 ctgggcgatg atttcatggg gcgcgacgtc tatagccgca tcgtctatgg cgcgcgcatc
     2521 tcgcttgccg tgggcctggg ctcgaccgcg ctcggctgcg ccatcggcgt cttcctgggc
     2581 ctcgcctcgg gctatctcgg cggctgggtc gacctcgccg tgcagcgcct cgtcgacatc
     2641 ctgcaaggcc tgccgctcct ggtgctggcg ctggtcatgg cggcatcgct cgggccgtcg
     2701 ctgcgcaaca ccatcatcgc cattgccgtg ccgctcatcc cctatgcggc gcgcgtcatc
     2761 cgcgccaaca ccctggcgct gcgcgagctg cccttcgtcg aggcggcgcg cgccacgggc
     2821 atgagcgagc tgcgcattgc gctccgccac gtgctcccca acacgctggc gccgctcatc
     2881 gtgctggcga cggcgcagct gggcgcgg
//
LOCUS       DATNAE010000188         2145 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3485174, whole genome shotgun sequence.
ACCESSION   DATNAE010000188 DATNAE010000000
VERSION     DATNAE010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3485174"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..2090)
                     /locus_tag="VN518_04155"
     CDS             complement(105..2090)
                     /locus_tag="VN518_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HXE03799.1"
                     /translation="MIAETGHYALILALGVAIVQTLLPLLGARFRDPQLAAVAAPAAQ
                     MQFVLVAIAFIALMTAYVTSDFSVENVWRNSHSSKPLLYKISGVWGNHEGSMILWVLI
                     LTLFGAAVATFGSNLPGQLKANVLAVQGSIGAAFLLFIITTSNPFTRLVPAPFDGEGL
                     NPVLQDPALAFHPPFLYTGYVGFSMAFSFAVAALIEGRIDAAWARWVRPWTLAAWMCL
                     TLGIAMGSWWAYYELGWGGFWFWDPVENASFMPWLAGTALLHSALVMEKREALKVWTI
                     LLAIITFSLSLMGTFLVRSGVLTSVHAFAVDPARGIFILAIMGVFIGGALTLFAWRAP
                     LLQQGGLFAPISREGALVFNNLLLTTACAAVFVGTLYPLALEALTGDKISVGPPFFNL
                     TFAPLIVPLLIAVPFGPLLAWKRGDLLAAAQRLMAAACVALGAAILLLAFNHRGPWLA
                     PLGIGLGIWLIMGALTEIGYRVKLLQNAPAEAWHRLRHLPRAAYGTALAHSGLGLAVI
                     GIVATTAWRSEAILAMKPGETADIAGYTLAFKGVAPRQGPNYRERVALFEVTRGGEAV
                     TELTPSKREFNVERSATTEAGIHVSWRGDLYAVLGDQLKDGAYSVRLYFNPLVRLIWL
                     GALVMFFGGAVSLSDRRLRVGAPKRAQAKAIQAAAGK"
BASE COUNT          441 a          700 c          714 g          290 t
ORIGIN      
        1 aagcacctcg tcgggctgga ccgcgcgagc cccggtggca agcagtatgt acagcgtgag
       61 cgcaaggaaa accaatgaga gaatgttgcg agcggccatg aagatcattt gccggcggca
      121 gcttgaatcg ccttcgcctg cgcccgcttc ggcgcgccga cccgcaagcg ccggtctgag
      181 agcgacaccg cgccgccgaa gaacatcacc agcgcgccga gccagatgag gcgcaccagc
      241 ggattgaaat agagacgtac gctgtaggcg ccgtctttca gctgatcgcc gagcacggca
      301 tagagatcgc cccgccagct cacgtggatg ccggcttcgg tggtggcgct ccgctcgaca
      361 ttgaactcgc gcttcgacgg cgtgagctcg gtgaccgcct cgccgccgcg cgtcacctcg
      421 aacagcgcca cccgctcgcg atagtttggc ccttggcgag gtgcaacacc cttgaaggcg
      481 agcgtgtaac cggcgatgtc ggcggtctcg ccgggcttca tcgccaggat cgcttccgac
      541 cgccatgccg tggtggcgac gatgccgatc accgcgagtc caaggccgga atgcgcgagc
      601 gccgtcccat aggccgcgcg cggcagatgg cgcagtcgat gccacgcttc tgccggcgcg
      661 ttctgcaaga gctttacgcg gtaaccgatc tcggtgagcg cgcccatgat cagccagatg
      721 ccaaggccaa tgccgagcgg cgcaagccat ggcccgcggt ggttgaaggc gagcagcaag
      781 atcgcggccc ccagcgccac gcaggcggcg gccatgaggc gctgtgccgc cgcaagcaaa
      841 tcgcctcgct tccaggcgag cagcggcccg aacggcacgg cgatcaagag aggcacgatc
      901 agcggcgcga aggtgagatt gaaaaacggc ggcccgaccg agatcttgtc gccggtcagc
      961 gcctcgagcg cgagcggata gagcgtgccg acgaacacgg cggcgcaagc cgtggtgagc
     1021 aggaggttgt tgaagaccag cgccccttcc cgactgatcg gcgcgaacag cccgccttgc
     1081 tgcaaaagcg gcgcgcgcca agcgaacagc gtcagcgccc cgccgatgaa cacacccatg
     1141 atggcgagga tgaaaatgcc gcgcgcggga tccaccgcga aggcatgcac cgaggtcagt
     1201 acgccggagc gaaccaggaa ggtgcccatt agcgacagcg agaaggtgat gatggcgagc
     1261 aggatggtcc acaccttaag cgcctcgcgc ttctccatga cgagcgccga atgcagcagc
     1321 gccgtgccgg cgagccaagg catgaaggag gcgttctcga ccgggtccca gaaccagaac
     1381 ccgccccagc cgagctcgta ataggcccac catgacccca tggcgatgcc gagcgtgagg
     1441 cacatccagg cggcgagcgt ccagggcctg acccagcggg cccaggcggc gtcgatgcgg
     1501 ccttcgatca acgcggcaac cgcgaaggag aaggccatcg agaagccgac atagccggta
     1561 tagagaaagg ggggatgaaa ggcgagcgcg gggtcttgca gcaccgggtt cagtccctcc
     1621 ccgtcgaacg gtgcgggcac cagccgcgtg aagggattgg acgtggtgat gatgaagagc
     1681 aggaaggccg ccccgatcga cccctgcacc gcgagcacat tcgccttgag ctggcccggc
     1741 aggttcgagc cgaaggtcgc caccgcggcg ccgaacaggg tgaggatcag cacccacaag
     1801 atcatcgagc cttcatgatt cccccacacg cccgagatct tgtagagcag cggcttcgac
     1861 gaatgcgaat tgcgccagac attctcgacc gagaagtccg aggtcacgta agccgtcatc
     1921 agcgcgatga aggcgattgc caccagcacg aactgcattt gcgcggcggg agcggcgacg
     1981 gcggcaagct gcggatcgcg gaagcgcgcg ccgagcagcg gcagcaatgt ctgcacgatg
     2041 gcgacgccaa gcgcgaggat cagcgcgtaa tgcccggtct cagcaatcat ctcccgttcc
     2101 gttcccgcga gttctagttc accttgccgg cctcgccgcc ctcgc
//
LOCUS       DATNAE010000189         5133 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_1087075, whole genome shotgun sequence.
ACCESSION   DATNAE010000189 DATNAE010000000
VERSION     DATNAE010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5133
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1087075"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="VN518_04160"
     CDS             <1..341
                     /locus_tag="VN518_04160"
                     /inference="COORDINATES: protein motif:HMM:NF037386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03800.1"
                     /translation="AANALPSQVPQEVKEERYARLMQHQQAISAEVLAERVGKTIEII
                     VDEVDTEGAIARSTWDAPEIDGNVYLNGETGLRPGDRARVSVEASDEYDLWGSVAEPV
                     AAQTPEPARA"
     gene            complement(403..1296)
                     /locus_tag="VN518_04165"
     CDS             complement(403..1296)
                     /locus_tag="VN518_04165"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HXE03801.1"
                     /translation="MSKDGYTLFIGDKNLSSWSLRPWIAMKHCGIPFAEERIRLRQPE
                     SREAILRHSPSGKVPALKANGLVVFDSLAILEYLAERHPELRLWPRDEEARALARSVS
                     AEMHSGFVTLRNDMSMELLARLPTPPISDELARDISRIVAIWRETRTKHGAGGPFLFG
                     AFSNADAMYAPVATRFRTYGVDLAAFGDDGTAASYAETIWHCLRWPSGRKARKQRSGR
                     GKQARPGALERRGQCAAKLGEHGNKRERKAGGNDAILDRAVVGVVSREQLDQSVHPAL
                     PAQPPELGVPRAPADMVAKAG"
     gene            1382..2800
                     /locus_tag="VN518_04170"
     CDS             1382..2800
                     /locus_tag="VN518_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012090979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HXE03802.1"
                     /translation="MSIRTLPKSRRDLGELLLERGSEALPIWFVAAKDDSQLQGLTEI
                     QRNWFEARGWSPKAGAVLLLPNESGGIAGALLGTGGEDWADQSPLLPGALPGALPPGD
                     YRFASKLPDPELATLAWLAGCYRFGRYKTENNGKPKRLVLPDGVERAKVLALAEALYF
                     GRDLINIPANDLGPAELEAAAREIGIEHGASIKVTEGSSLLSDNFPMIHAVGRASDRS
                     PRLIDLRWGSERAPKVTIVGKGICFDTGGLDIKPGSAMALMKKDMGGAAAALTFAHLV
                     MQAKLPLRLRVLIPAADNSIAGNAFRPGDVLRSRSGMTVEIGNTDAEGRLVLADALSL
                     ADEEAPDYLITIATLTGAARVALGPDLPPLYTNDEDLAAGLLASGASIGDPLWRMPLW
                     APYDKLIKSNIADVNHIAEGSHAGSVIAALFLKRFVTDAKRFAHLDIFGWVPREQPGR
                     PQGGEPQAARALFAYFRKEFGP"
     gene            2797..3681
                     /locus_tag="VN518_04175"
     CDS             2797..3681
                     /locus_tag="VN518_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HXE03803.1"
                     /translation="MTSGVLPDPRRHAYRASLAAESLRGRVEAPRYVEGVRRQVEASS
                     LPLRREPRFDAILDSEALFGETLTLFDESEGWAWVQLARDGYVGYLPSEGLTSAVTTP
                     THRVAALRTYVYASPDIKTPPLALLSLNALLSGAGEEGRFLALQSGGFVIAEHVRKLD
                     EPARDFVDVALDFRGTPYLWGGRTSLGIDCSGLVQLASEAAGLSCPRDADMQAGEVGR
                     QLDWQRADTLARGDLVFWDGHVGIMTSAQDLVHASAYQMMVVEEPLAEARARIASSKG
                     GEITGVRRLAGLSAAGHP"
     gene            complement(3678..>5133)
                     /locus_tag="VN518_04180"
     CDS             complement(3678..>5133)
                     /locus_tag="VN518_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014283443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXE03804.1"
                     /translation="LPYPAASHPSGVIRFKDEDLMALPLDGLRHVRGNQISMIFQEPM
                     TSLNPLHTIEQQIGEVLKIHRGLSNSAAQARVLDLIDKVGIEDPKSRLDSYPHQLSGG
                     QRQRVMIAMALANEPDLLIADEPTTALDVTIQAQILDLLLRLKSEFHMAMLLITHDLG
                     IVRKMADRVCVMTNGEIVERGTTHDIFASPQHPYTKHLLASEPKGSPPAANAKAPVIL
                     EAKDLKVWFPIKRGFMRRIVGHIKAVDGIDLAVKEGQTLGVVGESGSGKTTLGLALLR
                     LVSSEGPIVYLGNRIDGYDSKRMRPLRRDMQIVFQDPYGSLSPRLSVGQIIEEGLLIQ
                     KPGMDRTERRARVSRALEEVGLDPACQDRYPHEFSGGQRQRIAIARALVLEPKFLILD
                     EPTSALDVSVQAQIVDLLRDLQKRYKLAYLFISHDLKVVRALANAIIVLRHGKVVEQG
                     PARTVFAKPKTDYTKALLAAAFELEATRGDVVAT"
BASE COUNT          871 a         1671 c         1683 g          908 t
ORIGIN      
        1 ccgcggccaa tgcgcttccc agccaggtgc cccaggaggt gaaggaggag cgttacgcgc
       61 ggctgatgca gcatcagcag gcgatcagcg ccgaggttct ggccgaacgg gtcggcaaga
      121 ccatcgagat catcgtcgat gaggtcgata ccgagggcgc tattgcgcgt tccacctggg
      181 acgcgcctga gatcgacggc aatgtctacc tcaacggcga gacgggtttg cggcccggag
      241 accgcgcgcg cgtgagcgtc gaagcgtccg atgaatacga tttgtggggc agcgtcgccg
      301 agccagtcgc cgcgcaaacg ccagaaccgg cccgcgccta agcgctggcc ggtaaccctt
      361 cgttaactct cgcgcataat ttccgcagcg atttacgcgc cgctagccgg ccttcgccac
      421 catatctgcc ggtgcgcgcg gcacgcccaa ttctggtggt tgcgcgggga gggccggatg
      481 aaccgattgg tccagttgtt ctcgagaaac gacgccgaca accgcgcggt cgagtatggc
      541 gtcattgccg ccggccttgc gctcgcgctt gttgccgtgc tcgcccagct tggcggcgca
      601 ctgaccacgg cgctctaagg caccgggcct agcctgcttt ccgcgccctg atctctgctt
      661 ccgcgccttc cgtccactcg gccatctcag gcaatgccaa attgtttcgg cataggacgc
      721 tgcggtgccg tcgtcgccga aggcagcaag atccacgcca taggtgcgga atcgggtcgc
      781 caccggcgcg tacatggcat cggcgttcga gaaggcgccg aacaggaagg gaccgcccgc
      841 gccgtgcttc gtcctggtct cgcgccaaat cgccacgatg cggctgatgt cgcgggcaag
      901 ctcgtcgctt atcggcgggg ttggcagccg cgccaacagc tccatcgaca tgtcgttgcg
      961 cagcgtgacg aagcctgaat gcatctcggc cgagaccgaa cgggcaagcg cccgggcctc
     1021 ctcgtcccgc ggccacagcc gcagctcggg atgacgctcg gcgagatatt cgagaatggc
     1081 gaggctgtcg aacaccacga ggccattcgc cttaagcgcc ggaaccttgc cggagggaga
     1141 gtgacgcaag atcgcttccc tgctctcagg ctggcgcaag cggatgcgct cctcggcaaa
     1201 ggggatgccg caatgcttca tggcgatcca cggcctgagg ctccaggagg agaggttctt
     1261 gtcgccgatg aagagagtgt agccgtcttt cgacatgatc ccgccttgcg ccattgtcat
     1321 tgtggcgcac ccctgccatt aatgcggccc gccatcaagc cccgatccga accgttgatc
     1381 catgtcgata agaaccttgc cgaaatcgcg ccgtgacttg ggagagcttc tgcttgagcg
     1441 gggaagcgag gccctgccga tctggttcgt ggccgccaag gacgattcgc aactgcaagg
     1501 cttgaccgaa atccagcgca actggttcga agcgcgcggc tggtcgccga aagccggcgc
     1561 cgtcctgctc ctgcccaatg agagcggcgg catcgccggg gctctgctcg gcaccggcgg
     1621 agaggattgg gctgaccagt cgccgctact acccggcgcg ctccccggcg cgcttccgcc
     1681 cggcgattat cgcttcgcct cgaagcttcc ggaccccgag cttgcgaccc tcgcctggct
     1741 cgccggctgc taccgcttcg gccgttacaa gacagagaac aacggcaagc ccaagcgcct
     1801 ggtcctgccc gacggcgtgg agcgggccaa agtgctcgcc ctcgccgagg cgctctattt
     1861 cgggcgagac ctcatcaaca tcccggccaa cgatctcggc cccgccgagc tcgaagccgc
     1921 cgcgcgcgag atcggcatcg agcacggcgc ctcgatcaag gtgacggaag gatcgagcct
     1981 gctctccgac aattttccca tgatccatgc cgtaggccgg gcgagcgacc ggtctccgcg
     2041 gctgatcgat ttgcgctggg gctcggagcg cgcgccgaag gtcaccatcg tcggcaaggg
     2101 catctgcttc gacaccggcg gcctcgacat caagcctgga agcgccatgg cgctgatgaa
     2161 aaaggacatg ggaggagccg cagcagcact caccttcgcc catctcgtca tgcaggcgaa
     2221 gcttccgttg cgcttgcgcg tgctgatccc cgccgccgac aattcgatcg ccggcaacgc
     2281 cttcaggcct ggcgacgtgc tcaggagccg gagcggaatg accgttgaga tcggcaacac
     2341 cgatgctgaa ggccggttgg tgcttgccga cgcgctgagc ctcgccgatg aggaggcgcc
     2401 ggactatctc atcaccatcg ccacgctgac cggcgccgcg cgcgtggcac tcggccctga
     2461 tctgccgccg ctctacacca acgatgagga tttggccgct gggctgctcg cgagcggcgc
     2521 cagcatcggc gaccctctct ggcgcatgcc tctctgggcc ccctacgaca agctcatcaa
     2581 gagcaacatc gccgatgtga accatatcgc cgagggctcg catgcaggct ccgttatcgc
     2641 cgcgctgttc ctcaagcgct tcgtcaccga cgctaagcgc tttgcccatc tcgacatttt
     2701 cggctgggtg ccgcgcgagc agcctgggcg tccgcaaggc ggcgagccgc aagcagcccg
     2761 cgcgctcttc gcctatttcc gcaaagaatt cgggccgtga cgagcggcgt gctgccagac
     2821 ccgcgccgcc acgcttatcg cgcgagcctg gccgcggagt cgttgcgggg ccgtgtcgaa
     2881 gcgccgcgct acgttgaggg agtacgtcgc caggtcgaag cttcctcgct ccccttgcgc
     2941 cgggagccgc gcttcgatgc catcctcgac agcgaagcct tgttcggcga gaccctcact
     3001 ctgttcgacg agagcgaagg ctgggcctgg gtgcagctcg cgcgcgatgg ctatgtcggc
     3061 tacctgccga gcgaaggcct gacctccgcg gtcaccacgc cgacccatcg agtcgccgcc
     3121 ttgcggacct atgtctatgc gagccccgac ataaagacgc cgccgctcgc gctgttgagc
     3181 ttgaatgcgc ttttgtcggg tgctggcgag gagggcaggt tcctggcgct ccaatcaggc
     3241 ggcttcgtca tcgccgagca cgtgcgcaaa ctggacgagc ctgcgcgcga tttcgtcgac
     3301 gtggcgctag actttcgcgg cacgccctat ctgtggggcg ggcgcaccag ccttgggatc
     3361 gattgctcgg gcctggtgca gctcgcgagc gaagctgccg ggctttcctg cccgcgcgac
     3421 gccgatatgc aggcgggcga agtcggccgc cagctcgact ggcaacgcgc ggacacactc
     3481 gcccgaggcg atctcgtgtt ctgggacggc catgtcggta tcatgaccag cgcgcaggat
     3541 ctcgttcatg caagcgccta tcagatgatg gtggtggaag agcctctggc tgaagcccgg
     3601 gcgcgcatcg cctcctcgaa aggcggcgag attactggcg tgcggcgcct ggccggcctc
     3661 tcggccgccg ggcaccctta agtcgcgacc acgtcgccgc gcgtcgcttc aagctcgaac
     3721 gcagcggcaa gcagagcctt ggtgtagtcg gtcttcggct tggcgaacac ggtgcgcgcc
     3781 gggccctgct ccaccacctt gccgtgacgg agcacgatga tggcgttggc gagcgcgcgc
     3841 acgactttca gatcgtggct gatgaagagg taggcgagct tgtagcgctt ctgcagatcg
     3901 cgcaggagat cgacgatctg cgcctgcacc gacacgtcga gcgccgaggt cggctcgtcg
     3961 aggatgagga atttcggctc gagcaccagt gctctggcaa tggcgatgcg ctggcgctgg
     4021 ccgccggaga actcgtgcgg ataccggtcc tgacaggcgg gatcgagccc gacttcctcc
     4081 agcgcccgcg acacgcgcgc ccgccgctcg gtgcggtcca tacctggctt ctggatgagc
     4141 agaccttctt cgatgatctg ccccacggaa aggcgagggg agagcgagcc gtaggggtcc
     4201 tggaacacga tctgcatgtc gcggcggagc ggccgcatgc gcttcgagtc gtagccgtcg
     4261 atgcggttac cgagatagac gatcgggcct tcgctcgaca cgagcctgag caaagcgagg
     4321 ccgagcgtgg tcttgcctga tcctgactcg ccgacgaccc cgagcgtctg tccttccttc
     4381 accgcaaggt cgatgccgtc gaccgccttg atgtgcccga cgatgcggcg catgaagccg
     4441 cgcttgatcg gaaaccacac cttcaaatcc ttggcttcga ggatcaccgg tgccttggcg
     4501 ttggctgccg gcggcgagcc cttcggctcc gaggcgagga gatgcttggt gtaggggtgc
     4561 tgcggcgagg cgaagatatc gtgcgtcgtg ccgcgctcga cgatctcgcc gttggtcatc
     4621 acgcataccc ggtccgccat cttgcgcacg atgccgagat cgtgggtgat gagcagcatg
     4681 gccatgtgga attccgactt gagcctgagc agcagatcga ggatctgcgc ctggatggtc
     4741 acatcgagcg cggtggtggg ctcatcggcg atcagcaggt caggctcgtt ggcgagcgcc
     4801 atggcgatca tcacccgttg ccgctggccg cccgaaagct gatgcggata agagtcgagg
     4861 cggcttttcg ggtcctcgat accgaccttg tcgatgagat cgagcacgcg cgcctgcgct
     4921 gcgctgttcg acagaccgcg atggatcttg agcacctcgc cgatctgctg ctcgatggtg
     4981 tgaagcgggt tgagcgaagt cataggctct tgaaaaatca tggaaatttg attgccgcgg
     5041 acatggcgta gcccgtcgag agggagcgcc atcaaatcct cgtccttgaa tctgatgacg
     5101 cctgagggat gcgaggcggc gggatacggg agg
//
LOCUS       DATNAE010000190         2196 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_484411, whole genome shotgun sequence.
ACCESSION   DATNAE010000190 DATNAE010000000
VERSION     DATNAE010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484411"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /gene="nusA"
                     /locus_tag="VN518_04185"
     CDS             complement(<1..757)
                     /gene="nusA"
                     /locus_tag="VN518_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HXE03805.1"
                     /translation="MAQAGVSANRLELLQIADAVAREKSIDRQVVITAMEDAIQKAAK
                     SRYGSENEIKAEVDPKTGEIRLARLLEVVETVTMDATQVSLDEARRRNPAAQVGDFIA
                     EPLPPLDFGRVAAQNAKQVIVQKVREAERERQYDEFKDRVGEIVNGIVKRVEYGNVIV
                     DLGRAEAIVRRDETLPRENFRYGDRIRAYVYDVRREQRGPQIFLSRTHPEFMAKLFAQ
                     EVPEVYDGIVTIKSVARDPGSRAKIAVVSRDASI"
     gene            complement(775..1332)
                     /gene="rimP"
                     /locus_tag="VN518_04190"
     CDS             complement(775..1332)
                     /gene="rimP"
                     /locus_tag="VN518_04190"
                     /inference="COORDINATES: protein motif:HMM:NF000932.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HXE03806.1"
                     /translation="MSSASANEDRRFARETGPARAIADLAEPVLEELGFRLVRVKVSG
                     RDGGTVQIMAERPSGQMSVEDCATISRRLSPVLDAYDPMPGQYRLEVSSPGIDRPLVR
                     PSDFALWAGHEAKIELTELVDGRKRFRGLIEGVAKTEVRLKIELEGKAEPVTIGLPFS
                     LISEAKLVAGVDGFRQDLAGRKPAH"
     gene            complement(1507..1944)
                     /locus_tag="VN518_04195"
     CDS             complement(1507..1944)
                     /locus_tag="VN518_04195"
                     /inference="COORDINATES: protein motif:HMM:NF018684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HXE03807.1"
                     /translation="MDLRAFFVALPLLLPSGVVLAETDIGAVCYADCEAATHSNPEYK
                     ACLARAADKADAALNQAYKALQDNIRAAAKEMGQAPDVQLSDLKDAQKQWLAYRDSNC
                     TAEDSLAFGGTAIGGNYSSCLCALSYQRINDFERIRKKVLGLE"
     gene            complement(1959..>2196)
                     /locus_tag="VN518_04200"
     CDS             complement(1959..>2196)
                     /locus_tag="VN518_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HXE03808.1"
                     /translation="LAEIARVLRPGGAFRFASDNGDYAAQALRLARQSDLFTWTAERA
                     TDWRERPADWPETRYERKALSEGRKSTYLSFSRR"
BASE COUNT          373 a          718 c          681 g          424 t
ORIGIN      
        1 cgatggaagc gtcgcgcgac accaccgcga tcttggcgcg gctgcccgga tcgcgcgcca
       61 ccgacttgat ggtgacgatg ccgtcataga cctcgggcac ctcttgggcg aacagcttgg
      121 ccataaactc cggatgggtg cgcgacagga agatctgcgg tccccgctgc tcgcggcgca
      181 cgtcatagac ataggcgcgg atgcggtcgc cataacggaa attctcgcgc ggcaaagtct
      241 cgtcgcggcg gacgatggcc tcggcccggc cgagatcgac gatgacgttg ccgtattcga
      301 cccgcttgac gatgccgttg acgatctcgc cgacgcggtc cttgaattcg tcgtattggc
      361 gctcgcgctc ggcctcgcgc accttttgca cgatcacttg cttggcgttc tgcgccgcga
      421 cgcgcccgaa atcgagtggg ggcaggggct cggcgatgaa gtcgccgact tgcgccgcgg
      481 gattgcgccg ccgcgcctca tcgagcgaga cctgcgtcgc gtccatggta acggtctcga
      541 ccacttcgag caggcgcgcg agcctgatct cgccggtctt gggatcaacc tcggccttga
      601 tctcgttctc gctgccatag cgagatttcg ccgccttctg gatcgcatcc tccatggcgg
      661 tgatgaccac ctggcggtcg atcgacttct cgcgcgcgac cgcatcggcg atctgcaaga
      721 gctccagcct gttggcgctg acgcctgcct gagccatctc gttagcctcc aatctcagtg
      781 tgcgggcttc ctgcccgcaa ggtcttgtct gaagccgtcg acgcctgcga cgagcttcgc
      841 ttcgctgatg agcgagaagg gaaggccgat cgtcaccggc tcggccttgc cttctagctc
      901 gatcttcaaa cgcacctcgg ttttcgccac gccttcgatc agacccctga accgcttcct
      961 gccgtccacc aattcggtaa gctctatctt ggcttcatga cccgcccata acgcaaagtc
     1021 gctgggtcgg accaagggtc ggtcgatccc cggcgaagac acctccagcc ggtactgccc
     1081 aggcatcggg tcataagcgt cgagcacggg cgagagcctc cggctgatcg tcgcgcaatc
     1141 ctccacgctc atctgcccgc tcggccgttc ggccatgatc tgcaccgtgc cgccgtcgcg
     1201 gcccgagacc ttgaccctga ccagtctgaa gccgagctct tcgagcacgg gctctgccag
     1261 gtcggcgatg gcccgcgccg gtcccgtctc gcgggcgaag cggcgatcct cgttggcgct
     1321 ggcactcgac atcgttggtg agccgtggcc tcggcaaaac ggcgaggaag cgatgagcct
     1381 aacaaaaagg agcgggcgag aggcccactc ctgatcaaaa ccaatcatga gttcgagccc
     1441 taatatacgg gctttttcgg ggctttcaag cgcttcgatg ggccgaaaag ggctttcgtc
     1501 ggggtgttac tccaggccca gcaccttttt ccggatgcgc tcgaagtcat tgatgcgctg
     1561 ataggagagc gcgcacaggc aagaagagta attgccgccg attgccgtgc cgccaaaggc
     1621 gagactgtcc tccgccgtgc aattgctgtc gcgataggcg agccactgct tttgcgcgtc
     1681 cttgaggtcg ctgagctgaa cgtccggcgc ttgccccatc tccttggccg ccgcccggat
     1741 attatcctgc aacgccttat aggcttggtt cagcgcggcg tcggccttgt cggccgcgcg
     1801 ggcgaggcag gctttgtatt cgggattgga atgcgtggcc gcctcgcaat ccgcataaca
     1861 gacagcgccg atatcggttt cggcgaggac gacccccgag gggaggagca acggcaaagc
     1921 gacaaaaaac gcgcgaaggt ccatttcgct ctccaagatc aacgccggct gaaactgaga
     1981 taagtcgact tgcgtccctc gctgagcgcc ttgcgttcgt agcgcgtttc gggccagtcg
     2041 gctggccgct cccgccaatc ggtcgcccgc tcggcggtcc aggtgaaaag atcgctctgc
     2101 cgcgcgaggc gcagcgcttg cgcggcatag tcgccgttgt cggaggcaaa tctgaatgcg
     2161 ccgccgggac gcagcacgcg cgcgatctct gcgagc
//
LOCUS       DATNAE010000191         2689 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_1994739, whole genome shotgun sequence.
ACCESSION   DATNAE010000191 DATNAE010000000
VERSION     DATNAE010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1994739"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1827)
                     /locus_tag="VN518_04205"
     CDS             complement(<1..1827)
                     /locus_tag="VN518_04205"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HXE03809.1"
                     /translation="MAELIFALLSLAALFALAMSRAPLWAWAAAIAVFTLSLQMGLGS
                     GHLHAPVFNVWAVLGWLAAGGLFALTFPRLKHDYVVMPAYRALKGAMPTISETEREAL
                     EAGTVGWDAELFSGTPDWSRLRRVSPITLTAEERAFLDGPTEELCRKLNDWRIRHELH
                     DVPEDVWRFVRDQGFLGMLISKEHGGLGFSAQAQSLVLGKISSRSPDGVTIVMVPNSL
                     GPGELIEKYGTDAQKAHYLPRLARGDEIPCFALTGPFSGSDAASMRDIGVVTKGWYEG
                     NETLGIELTWDKRYITLAPNATLLGLAFRLFDPENLLGRGEDVGITLVLIPTDHPGVE
                     IGRRHLPAGAAFPNGPTSGKDVLVPIDWIIGGKERAGQGWRMLMSCLAAGRSISLPAT
                     GAAGTKSLLRTTTAYARIRKQFNLPIGYMEGVEEPLARMVENAYGLEAARAVTASMVS
                     AGEKPAVISALLKYMSTDRMRQSVNDALDIHGGRGICDGPSNYIQGAYQMVPVGITVE
                     GANILTRTLITFAQGALRSHPYLFTEIEALQDPDQDLGMPAFERAFDGHVAFTVSNIF
                     GALFHNLTLGAFASAPPNAGPTRRWWKQLARASRSFALVADAT"
     gene            complement(2225..>2689)
                     /locus_tag="VN518_04210"
     CDS             complement(2225..>2689)
                     /locus_tag="VN518_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein dehydrogenase-associated SoxYZ-like
                     carrier"
                     /protein_id="HXE03810.1"
                     /translation="RIETFSHVRAILETADGSLHMAIKFVQAAGGCAAMNAKDPDSEN
                     ADLGKMLVKTFPPALSTAPLFEAQVMIKHPNSNGMQLDIDTGGFIPARFIKEMTVKRG
                     NDLVFRMESTFSISTNPNFRFTFGRGADNELDVSVVDTDGTVFTAKSQASGS"
BASE COUNT          477 a          823 c          920 g          469 t
ORIGIN      
        1 ggtcgcgtcg gcgacgaggg cgaaggagcg cgaggcgcgg gcaagctgct tccaccagcg
       61 ccgcgtcggg ccggcgttgg gcggggcgct agcgaatgcg ccaagggtga gattatggaa
      121 gagcgccccg aagatgttcg acacggtgaa ggccacatgg ccgtcgaagg cgcgctcgaa
      181 cgcaggcatg ccgagatcct gatcggggtc ctgcaacgcc tcgatctcgg taaagagata
      241 agggtgactg cgcagcgccc cttgggcgaa ggtgatcagc gtgcgggtaa gaatattggc
      301 gccttcgacg gtgatgccca ccggcaccat ctgataggcg ccctggatgt agttggacgg
      361 cccgtcgcaa atgccgcggc ctccgtgaat gtcgagcgca tcgttcacgc tctgccgcat
      421 gcggtcggtc gacatgtatt tgagcagcgc cgagatcacc gctggcttct cgccagctga
      481 gaccatggaa gcggtgacgg cgcgcgcggc ctcgagccca taggcattct ccaccatgcg
      541 cgccaacggc tcttccacgc cctccatgta gccgatcggc aggttgaact gcttgcggat
      601 gcgggcgtag gccgtggtgg tgcggagcag agatttggtt ccggcggcgc ccgttgcggg
      661 caacgagatg gagcggcctg cggcgaggca gctcatcagc atgcgccagc cctgccccgc
      721 ccgctccttg ccgccgataa tccagtcgat cggcacgagc acatccttgc cggaggtcgg
      781 accgttgggg aaggcggcgc ctgccggaag atgccgccgc ccgatctcaa cgccgggatg
      841 atcggtcggg atgagtacga gcgtgatgcc gacatcctcg ccgcgtccga gcaggttttc
      901 gggatcgaac aggcggaagg cgaggccgag cagcgtggcg ttgggcgcga gggtgatgta
      961 gcgcttgtcc caggtaagct cgatgccgag cgtctcattc ccttcgtacc agcctttggt
     1021 gaccacgccg atatcgcgca tcgaggcggc atcggagcct gagaacggtc cggtgagggc
     1081 aaagcaggga atctcgtcgc cgcgcgcgag ccgcggcagg taatgcgcct tttgcgcatc
     1141 ggtgccgtat ttctcgatca gctcgcctgg cccgagagag ttcggcacca tgacgatggt
     1201 gacgccgtcg gggctgcgcg atgagatctt gccgagcaca agcgattgcg cctgcgccga
     1261 gaagcccaag ccgccatgct ccttggagat gagcatgccg aggaagccct gatcgcgcac
     1321 gaaccgccag acgtcttcag gcacgtcgtg cagctcgtgg cggatgcgcc agtcgttgag
     1381 cttgcggcaa agctcctcgg tgggaccgtc gaggaaggcg cgctcttcag cggtgagggt
     1441 gatggggctg acgcggcgga gcctcgacca gtccggcgtg ccggagaaga gctcggcgtc
     1501 ccagccgacg gtgccggctt cgagcgcctc acgctcggtc tcggagatcg tgggcatggc
     1561 gcccttgagc gcgcgatagg cgggcatcac cacgtagtcg tgcttgaggc gcgggaaggt
     1621 gagtgcaaac aggccgccgg cggcgagcca accgagaact gcccagacat tgaacaccgg
     1681 cgcgtgcaga tggccggagc caaggcccat ttgcaggctc aaggtgaaga cggcaattgc
     1741 ggccgcccag gcccaaagcg gcgcacggct catggcaagc gcgaacagcg ccgcaagcga
     1801 cagtagcgcg aagatgagct cagccatggg gactctccga ccgacccttt gcccctgcct
     1861 gccctgcgcc cgacggtatc aaagccattg ttcttgtgac ggacctcgat tcggtccgtt
     1921 cggccttatg acttctatgg tccggctccg tcatcgactc aagctctttt ctggcttgct
     1981 agacctattg gcaagacggg ctggagcgac gccaagcagg cctcatgccc ctcttcgcag
     2041 gccaagtcat accagccgat taacggcggg agccgttgcc cccgtcaggt tgccgcgccg
     2101 gcagcttgct cgttgctgcg gctcatgcgg cggtcgatgc cgatcagagc gaggccgagc
     2161 cctaggaaca cgagcaccag cacggccatg ttgatgagcg cgctggaaaa cggctttgcg
     2221 gaaattacga gccgctcgct tgcgacttcg ccgtgaacac ggtgccgtcg gtgtcgacca
     2281 cggagacgtc gagctcgttg tcggctcccc gcccgaaggt gaagcggaaa ttcgggttgg
     2341 tcgagatcga gaaggtgctc tccatgcgga acacgagatc gttgccccgc ttcaccgtca
     2401 tttccttgat gaagcgtgcc gggataaagc ctccggtgtc gatgtcgagc tgcatgccgt
     2461 tcgagttcgg gtgcttgatc atcacctgcg cctcgaacag cggcgcggtg gagagcgccg
     2521 gcgggaaggt cttcaccagc atcttgccga gatcggcatt ctcgctatcg gggtccttgg
     2581 cgttcatcgc ggcgcagccg cctgccgcct gcacgaattt gatggccatg tgcaagctgc
     2641 cgtccgcagt ctcgaggatg gcgcgcacat gggagaaagt ttcgatgcg
//
LOCUS       DATNAE010000192        19989 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_1383746, whole genome shotgun sequence.
ACCESSION   DATNAE010000192 DATNAE010000000
VERSION     DATNAE010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 19989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19989
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1383746"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /gene="ybeY"
                     /locus_tag="VN518_04215"
     CDS             <1..367
                     /gene="ybeY"
                     /locus_tag="VN518_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019994956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HXE03811.1"
                     /translation="TDDAEMRALNRAWRGKDSSTNVLSFPASYAPGAIAGEPRPLGDV
                     VLAAETVLAEAADRHIPAANHAAHFVVHGMLHLLGFDHERDEDAEAMEALETKVLSTI
                     GIADPYAEDARAGPAEVSP"
     gene            364..1350
                     /locus_tag="VN518_04220"
     CDS             364..1350
                     /locus_tag="VN518_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HXE03812.1"
                     /translation="MSNAPLSDDELPAASDQSPEEEPSWLERVLARFGLGEEPDLREV
                     IEDALTRAKGAGDTFSGQERAMLLKTLRFGKLTVEDVMVPRADIIAVEESMTVAELMA
                     VFREAEHSRLPVYRETLDDPRGMIHIRDLMSWITAAAEKSGAPSLDLGAVDLASTVGS
                     IDITREILYVPPSMPVLDLLLRMQLSRRHLALVVDEYGGTDGLVSIEDLVEEVVGEIE
                     DEHDVAEEAMIRQDPKLGLIADARTPIEDLARHLGVELATPEQEEDIDTIGGLVFSLA
                     GRIPARGELVRHPSGIEFEVLEADPRRIKKLRIHKAQERKVSPTETPAPESS"
     gene            1351..3018
                     /gene="lnt"
                     /locus_tag="VN518_04225"
     CDS             1351..3018
                     /gene="lnt"
                     /locus_tag="VN518_04225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HXE03813.1"
                     /translation="MLLAPVQAAADWVATLKGWKRALLAAFAGAISVLAFAPLHVWPV
                     LFVSFGLLVWLLDGCHAGHARLGTRLRCTTLIGFWFGFGYFLTGLYWTAEAFLVEPWR
                     HGWLIPFAMTGLAGGMALFYAAAAALAAILWRPGAARVFAMAIAFGLAEFARGHVLTG
                     LPWNLVGYGLAATLPMMQAASIFGVYALSLLALLLFASFFAIWAPQGSGLARPISTAL
                     VALFFSVALASGAMWGEARLASAGAATTGVRLRIVQANVDQANKWRPENSAEIFNTYL
                     DLTKSGAGAQGLDGITLVVWPETAVPFLLADSSEALHEIGDVLPEGTSLLVGSARVVA
                     ERDADGRLATERVYNSLIVVSDKGEILGSYDKVHLVPFGEYLPFQDFMESLGFLQLTG
                     IRGGFSAGTGSRLLSIPGAPLARPLICYEIIFPDEVADNSQRPGWFLNVTNDAWFGTS
                     AGPYQHFHQAQLRAVEQGLPVVRAANTGISAIIDPYGRVLGELGLKQEGVVDGLLPEA
                     LPITKFVMWGRWVELAVLSLALVGYLAFSRRFGNQSGPLRNQGIAGI"
     gene            3184..3597
                     /locus_tag="VN518_04230"
     CDS             3184..3597
                     /locus_tag="VN518_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003527670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXE03814.1"
                     /translation="MAGKKPNPVDQHVGSRVRLRRMLLGMSQERLGESMGLTFQQVQK
                     YEKGVNRIGASRLFQISKILDVPVQFFFEEAPHVDGRRAPGMAEGDSEAFILEFLNSR
                     EGLELNRAFVKISDPKVRKSVVDLVRALSASSDNA"
     gene            3693..4871
                     /gene="metK"
                     /locus_tag="VN518_04235"
     CDS             3693..4871
                     /gene="metK"
                     /locus_tag="VN518_04235"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HXE03815.1"
                     /translation="MANSRNYLFSSESVSEGHPDKVCDRISDAVVDLYMAADPHSRVA
                     IETLVTTNRIVMAGEVRGPESVTPALIEDAARRAVREVGYEQKGFHWKNAEFEFYVHE
                     QSADIAMGVDPNAEKEKEEGAGDQGIMFGYACRETEPLMPAPIYYAHRILHEMAKARH
                     TGRVPELGPDAKSQVTLAYEDGKPVRADSIVVSTQHGEDVSQERVQEIVRGFIDEVMP
                     QGWVGRDTKFYVNPTGRFVIGGPDGDAGLTGRKIIVDTYGGAAPHGGGAFSGKDPTKV
                     DRSAAYAARYLAKNVVAAGLADRCTLQVCYAIGVSEPLSLYVDLHGTGKIGESELEQA
                     LRRVMDLSPRGIREHLGLGRPIYERTAAYGHFGRQPDNDGGFSWERLDLIEPIRAELS
                     "
     gene            5002..5703
                     /gene="trmB"
                     /locus_tag="VN518_04240"
     CDS             5002..5703
                     /gene="trmB"
                     /locus_tag="VN518_04240"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015932333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HXE03816.1"
                     /translation="MPDQQMQRGDRLRSYGRRKARPLSERKDRLLNELLPTLRVPVRE
                     PAPSPLASLFRQPVRQVWLEIGFGSGEHLVWQAEHHPDIGFIGCEPFINGVASLLGKI
                     ETLGLNTIRIDDGDARDVLAWLPPNSISRIFILFPDPWPKKRHQKRRLVSPDAVADFA
                     RVLRQDAELRFASDSGDYAGEALLTIPASRAFAWTAERPKDWRVRPFDWPETRYERKA
                     LSEGRKPAYLSFRRL"
     gene            5733..6161
                     /locus_tag="VN518_04245"
     CDS             5733..6161
                     /locus_tag="VN518_04245"
                     /inference="COORDINATES: protein motif:HMM:NF018684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HXE03817.1"
                     /translation="MRAPFLVLMLLSTPALALDQGQIRTACYTDCEKETGSNPDYKAC
                     LARAADTADALLNQEFSALQDAVKAAAAEMEVRPEAQLDMLKDAQRKWIGFRDDTCNF
                     EDSLAFGATATGGYLSSCLCALSYERINDFDRIRHSIFGR"
     gene            6313..6867
                     /gene="rimP"
                     /locus_tag="VN518_04250"
     CDS             6313..6867
                     /gene="rimP"
                     /locus_tag="VN518_04250"
                     /inference="COORDINATES: protein motif:HMM:NF000932.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HXE03818.1"
                     /translation="MSSRSSSADRRFARETGAAREIADLAEPALEELGFRLVRVKVSA
                     RDGGTVQVMAERPVGQMTIDDCAEISRRLSPLLDAHDPMPGGYRLEVSTPGIDRPLVR
                     PSDFASFTGYEAKIELKELIDGRKRFRGRIEGVKNSEALLRIDLPGSEEPQVIGIPFS
                     LISEAKLVADQAGFRADLAASKAH"
     gene            6919..8565
                     /gene="nusA"
                     /locus_tag="VN518_04255"
     CDS             6919..8565
                     /gene="nusA"
                     /locus_tag="VN518_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HXE03819.1"
                     /translation="MAQAGVSANRLELLQIADAVAREKAIDRQVVIMAMEDAIQKAAK
                     SRYGSENEIKAEVDPKTGEIRLARLLEVVEQVTMEATQISIEEARCRNPAAQVGDFIA
                     EPLPPLDFGRVAAQNAKQVIVQKVREAERERQFDEYKDRVGEIVNGIVKRVEYGNVIV
                     DLGRAEAIVRRDETLPRENFRYGDRIRAYVYDVRREQRGPQIFLSRTHPEFMAKLFAQ
                     EVPEVYDGIVTIKSVARDPGSRAKIAVVSRDASIDPVGACVGMRGSRVQAVVNELQGE
                     KIDIIQWSPDAATFIVNALAPAEVVKVVLDEDAERIEVVVPDAQLSLAIGRKGQNVRL
                     ASQLTGWDIDIMTEASESERRQAEFAERSSTFMEALDVDEVIAQLLASEGFSSVEEVA
                     FVEPSEIASIEGFDENTAAEIQTRAREHLEKIEAEQDAKRKALGVFDEVATIPGLTTA
                     MLVALGEKKVKTVEDVADCATDDLLGWNERKDKETIHHEGILDGFNLSKQEIEDLILA
                     ARVKAGWIDEADLAPEPEAEEADSGEGEEVGETKDGGAAS"
     gene            8606..9283
                     /locus_tag="VN518_04260"
     CDS             8606..9283
                     /locus_tag="VN518_04260"
                     /inference="COORDINATES: protein motif:HMM:NF006622.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HXE03820.1"
                     /translation="MRQDVAEPQLEAESETGDTVRRCALTRMRLPKQDLIRFVLAPTG
                     EIVPDLKERLPGRGVWVTADHAVVAEAAKCNVFARALKAQAKVPPGLAAQVDRLLVEA
                     ALGALALANKAGEVVFGTAKIEEAIAKAKVAALIHASDAAEDGCRKLDGRFRAVARGE
                     TPAPIRLFSADELGLASGKTNVIHAALIQGGAAAKFLASASRAERYRKGSAAAFAQPI
                     GSDTDKE"
     gene            9280..11775
                     /gene="infB"
                     /locus_tag="VN518_04265"
     CDS             9280..11775
                     /gene="infB"
                     /locus_tag="VN518_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011583238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HXE03821.1"
                     /translation="MSEINETEDKTARGGRKMTLNVRRTVESGHVRQSFSHGRSKSVL
                     VEKKKRRAVGATPAAQPVEEPKPKVATAPEPTPAKHARDDEQRRPGVVLRALSDEEKD
                     ARLRALTESRQREAIEREKAKEEAAKRVVTEEVKAKEHDETLERQKKEEARHEAEAEA
                     RRKGEEVARKHLVVKEEEEEAPRTQEARHKKERAVKARAGEERRVRGRLTITNALDEE
                     QRQRSLASLKRHRERQKKQTIGPQAQLKIQRDVVIPEVITIQELANRMAERGVDVIKY
                     LMKDGEMHKITDVIDSDTAQLIAEEFGHVVRRVSEADVEEGFIGAEDIAEDVEPRAPV
                     VTIMGHVDHGKTSLLDAIRATNVVSQEAGGITQHIGAYQVVTPAGQKITFIDTPGHEA
                     FTAMRQRGAQVTDIVVLVVAADDGVKPQTVEAINHAKAAQVPMIVAINKIDKPGADPQ
                     RVRTELLSHEIVVESLGGDTLEIEVSALKKTGLDRLLEAIALQAEVLELKANPNRPAE
                     GVIVEAKLERGRGPVGTALVQRGTLHLGDLVVAGSAYGRVRALINDLGEQVKDAGPSV
                     PVEVLGFDSAPEAGDQFAVVENEARAREITDYRLRERRKRQAVAGQRGSLEQMMTQLK
                     AAGTKEFTLLVKGDVQGSVEAIVAALDKLGTDEVRARIIHAGVGGITESDVGLASTSG
                     AVILGFNVRANSQAREAAERDGVEIRYYAVIYDLVDEVKQAMSGLLAPTRRETFLGNA
                     EILEVFTISKVGKVAGCRVTEGKVERGAQVRLIRDNVVVHEGKLSTLKRFKDEVKEVV
                     AGQECGMAFEGYQDMRQGDVIECFEVEHVRRTL"
     gene            11809..12201
                     /gene="rbfA"
                     /locus_tag="VN518_04270"
     CDS             11809..12201
                     /gene="rbfA"
                     /locus_tag="VN518_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HXE03822.1"
                     /translation="MTDRHRGKAPSQRQLKVGETIRHALAEIFARGEIVDEVLDQYSL
                     TVSEVRMTPDLKLATAFVLPLGGEGAEDAVQHLDKHKRFLRGELARRVSLKFMPEIRF
                     KIDISFASSKRIDELLASPKVARDLDAD"
     gene            12218..13144
                     /gene="truB"
                     /locus_tag="VN518_04275"
     CDS             12218..13144
                     /gene="truB"
                     /locus_tag="VN518_04275"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013417768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HXE03823.1"
                     /translation="MARRRKGLPVHGWLIFDKPKGMTSTQAVTRVRHLYGAQKAGHAG
                     TLDPLATGVLPVAFGEATKTVPFAVDGDKQYRFTVRFGEATDTDDSDGTVIATSDRRP
                     SRTEIEAALPSFVGEISQVPPRYSALKVGGARAYDLAREDQDFELAPRRALIESLELT
                     DMPDPDHCVIEARCGKGTYVRALARDLGEALGCFAHVTELRRTRVGPFGEEAAISLAK
                     LEELGHSAAGRDALLGVLKPVQTALDDIPALAISGQDAARLKRGQPVLLRGRDAPILK
                     GPVYATSRGSLVAVGEVSQGELRPTRVFNLPG"
     gene            13176..13445
                     /gene="rpsO"
                     /locus_tag="VN518_04280"
     CDS             13176..13445
                     /gene="rpsO"
                     /locus_tag="VN518_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006473093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HXE03824.1"
                     /translation="MSISALRKQELIKDYATTPGDTGSPEVQVAILTERITNLTDHFK
                     THNKDNHSRRGLLKMVSQRRRLLDYVKATDEDRYQKLINRLGIRR"
     gene            13558..15681
                     /gene="pnp"
                     /locus_tag="VN518_04285"
     CDS             13558..15681
                     /gene="pnp"
                     /locus_tag="VN518_04285"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HXE03825.1"
                     /translation="MLHKHVVEIEWAGRPLRLETGRIARQADSAVLAQYGGTSVLATA
                     TAAKEAKPGIDFFPLTVNYQEKTFAAGKIPGGYFKREGRPTEKETLTSRLIDRPIRPL
                     FAGGFRNETQVVATVLSHDMENDPDIVALIASSAALTLSGIPFLGPIGAARVGYIAGK
                     YVLNPMIDEMRESTLDLVVAGTASAVLMVESEAKELPEDVMLGAVMYGQKHFQPVLDA
                     IVKLAERAAKEPWELKSDASAEELAAKARAIAEVELREAYKIAAKHERRDSVAAVKEK
                     VVTALAPESTDVTVIGKAASAFKELEKDVVRSSILATKHRIDGRDLTTVRPIVCEVGI
                     LPRTHGSALFTRGETQALVVATLGTGEDEQYIDALEGTYKETFMLHYNFPPYSVGEVG
                     RLGSPGRREIGHGKLAWRAIHPVLPDRETFPYTIRVVSEITESNGSSSMATVCGSSLA
                     MMDAGIPLRRPVAGIAMGLIKEEADFAVLSDILGDEDHLGDMDFKVAGTSEGVTSLQM
                     DIKITGITEEIMKVALDQAREGRMHILSEMNKALNEARPELGEYAPRIETIHIPVDKI
                     REVIGSGGSVIREIVAESGAKIDISDDGTVKIASANAESIRAAISRIKGIAAEPEVGE
                     IYHGKVVKVMEFGAFVNFFGAKDGLVHISQLAEGRPKAVTDVVKEGDMVWVKLLGFDD
                     RGKVRLSMKVVDQTTGRELQKAEAS"
     gene            complement(15745..15936)
                     /locus_tag="VN518_04290"
     CDS             complement(15745..15936)
                     /locus_tag="VN518_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03826.1"
                     /translation="MAQLVVLICLLSTPTDCSEKPVPGATATDVVSCLKTAGDKADEW
                     QKANSQYFVIGWRCEKQKS"
     gene            complement(15973..16854)
                     /gene="fabI"
                     /locus_tag="VN518_04295"
     CDS             complement(15973..16854)
                     /gene="fabI"
                     /locus_tag="VN518_04295"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HXE03827.1"
                     /translation="MTTTRDIAEPGPLMKGKRGLVMGVANDRSIAWGISRVLHGQGAE
                     LAFSYQGGAFGRRAVPLAQSIGAPIIEEADVEDLESVKRVFEVIRKKWGSLDFVVHAL
                     AFSDRNELKGRYADTTRANFEHTMVISCFSFTEIAKLASELMPNGGSLLTLTYGGSTR
                     VVPCYNVMGVAKAALEASVRYLATDLGPQAIRVNALSAGPMRTLAGAGISDARTLFHY
                     QQRHAPMHRSPTLDEVGGAALYLLSDLSGAVTGEIHFVDCGYSTISMPGLPALKSMEN
                     DKELKAAPEKAPREAAQ"
     gene            complement(16905..18119)
                     /gene="fabB"
                     /locus_tag="VN518_04300"
     CDS             complement(16905..18119)
                     /gene="fabB"
                     /locus_tag="VN518_04300"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HXE03828.1"
                     /translation="MRRVVVTGMGIVSSIGNSAQEVLASLREAKSGIVKADTYAELGF
                     RCQVHGAPKLDWEGMIDRKIRRFMGAGAAWNYLAMEQAIRDAGLEETDVSNERTGLIM
                     GSGGPSTKAIVQAADTTREKGPKKVGPFEVPKSMSSTNSATLSTPYHIKGLSYSISSA
                     CSTSAHCIGNGGELIQWGKQDILFAGGGEELDWTLSVLFDAMNAMSSDFNDRPEVASR
                     AYDVARDGFVIAGGAGVVILEELEHAKARGARIYGELVGYGATSDGYDMVQPSGEGAA
                     RCMRLAMQGVDGIDYINPHATSTPIGDVKEIEAIREVFGDKSPPISATKSLTGHSLGA
                     AGAHEAIYSLLMMNNGFICESANIETIDPAVADMPIVRKRKDNVDIDCVMSNSFGFGG
                     TNATLVFRRYNG"
     gene            complement(18170..18700)
                     /gene="fabA"
                     /locus_tag="VN518_04305"
     CDS             complement(18170..18700)
                     /gene="fabA"
                     /locus_tag="VN518_04305"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     FabA"
                     /protein_id="HXE03829.1"
                     /translation="MVERREQFDYEDLLACARGELFGPGNAQLPLPPMLMFDRISEVK
                     EEGGEHGYGRIVAELDVKPDLWFFPCHFKGDPVMPGCLGLDALWQLLGFFLGWLGAPG
                     KGRALSVGEVKFSGMVLPTMKKLEYIVELKRVIRRKFTLGTGDGWLKADGDTIYTAKD
                     LRVGLFHAGEEAPAGA"
     gene            18892..19311
                     /locus_tag="VN518_04310"
     CDS             18892..19311
                     /locus_tag="VN518_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006940145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HXE03830.1"
                     /translation="MTLPAMDIAERLRAAGLRPTRQRVALARLLFDGCDRHVTAELLH
                     EEAQSRKVPVSLATVYNTLHQFTEAGLLREVAVEGAKTYFDTNTSNHYHFFCENSGTL
                     LDIQTASIRIEGLPQAPDGMSISRVDVLVRLVDDRMR"
     gene            complement(19460..>19989)
                     /locus_tag="VN518_04315"
     CDS             complement(19460..>19989)
                     /locus_tag="VN518_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HXE03831.1"
                     /translation="LAAGVALAILFLAGIETRAANDASPATGSSSSQTRLPIPRFVSL
                     KSDRVNVRKGPSTDQAIVWVFSRAGLPVEIIAESDNWRRVRDSEGADGWVWHSLLSGR
                     RTVLISPWTKTPENVPLYSRKSTGASTVAELAPSVLGNVLSCDGEWCELSIDDYSGFV
                     QQDKLWGVYKDEVIK"
BASE COUNT         3728 a         6421 c         6426 g         3414 t
ORIGIN      
        1 gaccgacgac gccgagatgc gcgcgctgaa ccgtgcctgg cgcggcaagg actcctcgac
       61 caatgtgctg tcgtttcccg cgagctatgc tcccggcgcg attgctggcg agccgcgtcc
      121 cctcggcgat gtggtgcttg ccgccgaaac cgtgctcgcc gaggctgccg acagacacat
      181 tccggccgcc aatcacgccg cccatttcgt ggtgcacggc atgctgcatc tcctcggctt
      241 cgaccacgag cgcgacgaag acgccgaggc gatggaagcg ttggagacca aggtgctctc
      301 cacgatcggc atcgccgatc cctacgcgga ggacgcgcga gccggccctg ccgaggtatc
      361 gccatgagca acgccccgct ctccgacgac gagcttccgg ccgcgagcga tcaatcgcct
      421 gaggaggagc cgtcctggct cgagcgcgtg ctcgcccgct tcggtctcgg cgaggagcca
      481 gacttgcgcg aggtcatcga ggacgccctg acccgggcca agggcgccgg cgacaccttc
      541 agcgggcaag agcgcgccat gctgctcaag acgctgcgct tcggcaagct cacggtcgaa
      601 gacgtcatgg tcccgcgcgc cgacatcatc gccgtcgaag aatcgatgac cgtcgccgag
      661 ctgatggctg tgttccgcga ggccgagcat tcgcggctcc cggtctaccg cgaaacgctc
      721 gacgatccgc gcggcatgat ccatatccgc gacctcatgt cgtggatcac cgcggcggcg
      781 gagaagagcg gcgcgcctag cctcgatctc ggtgccgtcg accttgcgag caccgtgggg
      841 tccatcgaca tcacccgcga aattctctac gtgccgccgt cgatgccggt gctcgatctt
      901 ctgctgcgca tgcagctgtc gcggcggcat cttgccctcg tcgtcgacga atatggcggc
      961 accgacggtc tcgtctccat cgaggatctg gtcgaagagg tggtgggcga aatcgaggac
     1021 gagcatgacg tggccgagga agcgatgatc cgccaggacc cgaagctcgg acttattgcc
     1081 gatgcccgaa cgccgatcga ggatctcgcg cggcatctcg gcgtcgagct cgccacgccg
     1141 gagcaggagg aggacatcga caccatcggc ggtctcgtgt tctcgcttgc cggccgcatc
     1201 ccggcgcgcg gcgagctggt gcgccacccg agcggcatcg agttcgaggt gctggaagcc
     1261 gatccgcgcc gcatcaagaa gctgcgcatc cacaaggcgc aagaacgcaa agtgtcgcca
     1321 accgaaacgc cagcgcccga gagcagctga gtgcttttag cgcccgtgca agcggcagcc
     1381 gattgggtcg cgacgctgaa ggggtggaag cgcgctttgc tcgcggcctt cgccggcgcc
     1441 atctccgttc tcgcctttgc cccgctgcat gtctggccgg tgctgttcgt gagcttcggc
     1501 ctgctcgtct ggctgctcga cgggtgccac gccggccacg caaggcttgg cacgcggctc
     1561 cgctgcacca cgcttatcgg attctggttc ggcttcggct atttcctcac cggtctttat
     1621 tggaccgccg aagcctttct ggtcgagccc tggcggcacg gctggctcat ccccttcgcc
     1681 atgacggggc tcgccggcgg catggctctg ttctatgccg ccgctgcggc gcttgccgca
     1741 atcctgtggc gcccaggcgc ggcaagggtt ttcgcgatgg ctatcgcctt tggcctcgcc
     1801 gagttcgccc gcggccacgt gctgaccggg ctgccctgga acctcgtagg atacgggctt
     1861 gccgcaaccc ttcccatgat gcaggcggcc tcgatcttcg gcgtctacgc ccttagcctc
     1921 cttgcgctct tgctctttgc cagctttttc gccatctggg cgccgcaagg ctctggcctc
     1981 gcacggccca tcagcaccgc gctcgttgcg ctcttcttct cggtggcgct tgctagcggc
     2041 gcgatgtggg gagaggcgcg gcttgcgagc gcaggtgccg ccaccaccgg cgtgcggctc
     2101 cgcatcgtgc aggcgaatgt cgatcaggcc aacaaatggc gcccggagaa cagcgccgag
     2161 attttcaaca cctatctcga tctcaccaaa tccggcgcgg gcgctcaagg gctcgacggc
     2221 atcacgctcg tggtctggcc ggagacggcg gttcccttcc tcctcgccga ctcctcggag
     2281 gcgcttcacg aaatcggcga tgtgttgcct gagggcacga gcctgctcgt gggatcggcg
     2341 agagtggtcg ccgagcgcga cgccgacgga cgtcttgcca ccgagcgcgt ctacaacagt
     2401 ctcatcgtgg tcagcgacaa gggtgagatc ctcggcagct acgacaaggt ccatctcgtg
     2461 ccgttcggcg aatatcttcc gtttcaggat ttcatggaga gtctcggctt cttgcagctc
     2521 accggcatcc gcggcggctt cagtgccggc accggttctc gcctgctctc gatcccgggc
     2581 gcgccgctgg cgcggccgct catttgctac gagatcattt ttcccgacga ggtcgccgac
     2641 aattcgcaaa gacccggctg gttcttgaac gtcaccaacg atgcgtggtt cggcacgagc
     2701 gctggtcctt atcagcattt tcatcaggca caacttcgcg ccgtcgagca aggtttgcca
     2761 gtggtgcgcg cggccaacac cggcatctcg gcgatcatcg atccttacgg acgcgtcctc
     2821 ggcgagctcg gtctgaagca agagggagtc gtcgacggtc tcttgcccga ggctttgccg
     2881 attacgaaat tcgtaatgtg gggacgctgg gtcgaactcg cggttctctc acttgcgctt
     2941 gttggctacc tcgcattttc tcgtcggttc gggaaccaaa gtgggcccct gcgcaatcaa
     3001 ggaatcgcgg gcatttgaac actttgcaaa tgcctcgcaa cgcgaggata tgcagatgaa
     3061 gttgacatgc ggcgcctaag agcttgacgg ccctgggaac cacccctaat taaattgttg
     3121 atctggggga ctaaacaaag cgtcagtgat cgtacaacca acatgatatt ggtgagtgaa
     3181 cgcatggcag gcaagaagcc taacccggtg gatcagcatg tggggagccg ggtccgtttg
     3241 cgccgcatgt tgctcggcat gagccaagag cgactcgggg agagcatggg actcacgttt
     3301 cagcaggtgc agaaatacga gaagggtgtg aaccgcatcg gcgcgagccg gctgttccag
     3361 atctccaaga tcctcgacgt ccccgtacaa ttcttcttcg aggaagcacc gcacgtcgac
     3421 gggcgcaggg cgcccggcat ggcggaaggg gactctgagg ctttcattct cgaattcctc
     3481 aacagccgcg aggggctcga gctcaaccgc gccttcgtca agatctccga tccgaaagtc
     3541 cgcaagagcg tggtcgatct cgttcgggcg ctgagcgcga gcagcgataa cgcttaggcg
     3601 tcatcaggcg gcttgaccgc cacccctctt cttgtattaa gccggcgcgg gacacggtcc
     3661 cgtcctgcac cgtcggattc atagagggtc acgtggcgaa cagtcgaaac tatctcttca
     3721 gcagcgagtc ggtctcggaa ggtcaccccg acaaggtgtg cgatcgcatc tccgatgccg
     3781 tggtcgatct ctacatggcc gccgatccgc attcgcgggt ggcgatcgaa accttggtga
     3841 cgaccaaccg catcgtcatg gccggagagg tgcgcggacc tgaatccgtg acaccggctc
     3901 tgatcgagga cgccgcgcgc cgcgccgtgc gcgaggtcgg ctacgagcag aagggctttc
     3961 actggaagaa cgccgagttc gaattctacg tgcacgagca gtcggccgac atcgccatgg
     4021 gcgtcgatcc caatgccgag aaagagaagg aagagggcgc cggcgaccag ggcatcatgt
     4081 tcggctatgc ttgccgcgag accgagccct tgatgccggc gccgatttat tacgcccacc
     4141 gcatcctgca tgagatggcc aaggcgcggc acaccggcag ggtgcccgag cttggccctg
     4201 acgccaaaag ccaggtcacg ctcgcttatg aggacggcaa gccggtgcgc gccgactcga
     4261 tcgtggtatc gacccagcac ggcgaagatg tgagccaaga gcgggtgcag gagatcgtgc
     4321 gcggcttcat cgacgaggtg atgccgcaag gctgggtcgg ccgcgacacc aaattctacg
     4381 tgaatcccac cgggcgtttc gtcattggcg gccccgacgg cgacgccggg ctcaccgggc
     4441 gcaagatcat cgtcgacacc tatggcgggg cggcgccgca tggcggcggc gccttctcgg
     4501 gcaaggaccc gaccaaggtc gaccgctcgg cggcttatgc ggcgcgctat ctcgccaaga
     4561 acgtggtggc ggcggggctc gccgaccggt gcacgctgca ggtctgctac gccatcggcg
     4621 tgtccgagcc gctttcgctc tatgtcgatc tgcacggcac cggcaagatc ggtgaatccg
     4681 agctcgagca ggcgctcagg cgcgtcatgg atctgagccc gcgcggcatc agggagcatc
     4741 ttgggctcgg ccgcccgatc tatgagcgca ccgcggccta cggccatttc ggccggcagc
     4801 cggacaatga cggcggattt tcctgggagc gcctggacct gatcgagccg atccgcgccg
     4861 aactgagcta gacctctctc tttcttgtca tcgcccggct tgaccgggcg atccagtaac
     4921 ccctagcgga gatagtcctg ctagacctgt ggctactgga tgcccgcttt tgccggcata
     4981 gcaaaccggg agagcattgt tttgcctgat cagcaaatgc agcgtggcga ccgtctcagg
     5041 tcctacggcc ggcggaaagc gcggccgttg agcgagcgaa aagaccgcct gctcaacgag
     5101 ctgctgccga ctttgcgcgt tcctgtgcgg gagccggccc cctccccgct cgcgagcctg
     5161 ttcaggcagc ctgtcagaca ggtctggctc gagatcggct tcggctctgg cgagcatctt
     5221 gtgtggcagg ccgagcatca tcccgacatc ggcttcatcg gctgcgaacc cttcatcaac
     5281 ggcgtggcga gcctgctcgg caagatcgag actttgggcc tcaacaccat ccgcatcgac
     5341 gacggcgatg cccgcgacgt gctcgcctgg ctgccgccga attcgatatc gcgcattttc
     5401 atcctgttcc ccgacccttg gccgaagaaa cggcaccaga agcgaaggct cgtttcgccc
     5461 gatgccgtcg ccgatttcgc cagggtgctg aggcaagacg cggagctgag gttcgcgagc
     5521 gacagcggcg actacgccgg tgaggctctc ctaaccatcc ctgcaagccg ggcctttgcc
     5581 tggacggcgg aacggcctaa ggattggcgg gtgcggcctt tcgactggcc tgagactcgc
     5641 tacgagcgga aggccttgag cgagggccgc aaaccggcct atctcagctt ccgtcgcctc
     5701 tgaccgtgtt gcctcccgct gggaagaaat cgatgcgcgc tcctttcctc gtgctgatgc
     5761 tgctctcaac gccggcgctt gctctcgatc agggccagat ccgaaccgcc tgctataccg
     5821 attgcgaaaa ggagaccggc tcaaatccgg actacaaggc ctgccttgcc cgcgccgccg
     5881 acaccgccga tgcgctgctc aatcaggaat tctccgcgct ccaggatgcg gtcaaagcgg
     5941 cagccgccga gatggaggtg aggccggaag cccagcttga catgctgaaa gacgcgcagc
     6001 gcaagtggat cggatttcgc gacgacacgt gcaatttcga ggacagcctt gccttcggcg
     6061 ctaccgccac cggcggctat ctctcctcct gcctctgcgc cctaagctac gagcggatca
     6121 acgatttcga ccgtatccgc cactcaattt tcgggcgcta atagcccgca agcctccttg
     6181 aatcctttga aaaacaccgt atattaggcc ctaactcatg attggttttg atcaggagtg
     6241 ggccgttcgc ccgctccttt ttgttaggcg ccccgcttgc tcatttcgtc tgcgggagtc
     6301 atggctttat caatgtcgag ccgctcatca agcgctgacc gccgcttcgc acgcgagacg
     6361 ggcgccgcgc gcgagatcgc cgatctcgcc gagccggcgc tcgaggagct tggcttccgc
     6421 ctggtgcggg tgaaggtttc ggcccgcgac ggcggcacgg tgcaggtgat ggcggagagg
     6481 ccggtgggtc agatgaccat cgatgattgc gcagagatca gccggaggct ctcgccgctg
     6541 ctcgatgcgc acgacccgat gcccggaggc taccggctcg aggtctcgac gcctggcatc
     6601 gaccggcctc tggtgcggcc aagcgatttc gcgtcgttca ccggttatga ggcgaagata
     6661 gagctgaagg agctgatcga cggcagaaag cgattcaggg gccgcatcga aggcgtgaag
     6721 aacagcgagg cgctgctgcg gattgatctg ccaggctcag aggagccgca ggtgatcggc
     6781 attccgttct cgctgatcag cgaagcgaag ctggtcgccg atcaggccgg cttccgagcc
     6841 gatctcgccg ccagcaaggc gcattagaat tgtcgcgggc acccgcccgc cagttggact
     6901 taacggaggc agaaacagat ggctcaggca ggcgtgagcg ccaacaggct ggagctccta
     6961 cagatcgcgg acgcggtcgc gcgcgagaag gcgatcgatc gccaggtggt gatcatggcc
     7021 atggaggacg cgatccagaa ggcggccaag tcgcgctacg gcagcgagaa cgagatcaag
     7081 gccgaggtcg atccaaagac gggcgagatc aggctcgcgc ggctgctcga agtggtcgag
     7141 caggtgacca tggaggcgac gcagatctcg atcgaggagg cgcggtgccg caacccggcg
     7201 gcgcaagtcg gcgacttcat cgccgagccg ctgccgccgc tcgattttgg ccgcgtggcg
     7261 gcgcagaacg cgaagcaggt catcgtgcag aaggtgcgcg aggccgaacg cgagcgccaa
     7321 tttgacgagt acaaggaccg ggtcggcgag atcgtgaacg gcatcgtcaa gcgcgtcgaa
     7381 tacggcaacg ttatcgtcga ccttgggcgc gccgaggcca tcgtccgccg cgacgagacc
     7441 ttgccgcgcg aaaacttccg ctacggcgac cgcatccgcg cctatgtcta cgacgtccgc
     7501 cgcgagcagc gcgggcccca gattttcctg tcgcggaccc atcccgaatt catggccaag
     7561 ctgttcgcgc aggaggtgcc ggaggtctat gacggcatcg tcaccatcaa atcggtggcc
     7621 cgcgatccgg gcagccgcgc caagatcgcg gtcgtgtcgc gcgacgcctc catcgatccg
     7681 gtcggcgcct gcgtcggcat gcgcggcagc cgcgtgcagg cggtggtgaa cgagctgcag
     7741 ggcgagaaga tcgacatcat ccaatggtcg cctgacgccg cgaccttcat cgtcaatgcg
     7801 ctggcgcccg ccgaggtggt caaggtggtg ctcgacgagg acgccgagcg catcgaggtg
     7861 gtggtaccgg acgcgcagct ctcgctcgcc atcggccgca aaggtcagaa tgtcaggctt
     7921 gcctcgcagc tcaccggctg ggacatcgac atcatgacgg aagcgtctga gtccgagcgc
     7981 cgccaggccg agtttgccga gcgctcctcg accttcatgg aggcgctcga cgtcgacgag
     8041 gtcatcgccc agctgctcgc ttctgaagga ttctcctcgg tcgaagaggt tgccttcgtc
     8101 gaaccttcgg aaatcgcctc gatcgaaggc ttcgacgaga ataccgcggc tgagatccaa
     8161 acccgcgcac gcgagcatct ggagaagatc gaggccgagc aggacgcgaa gcgcaaagcc
     8221 ctcggcgtct tcgacgaggt ggcgaccatc cccggcctca ccacggcgat gctggtcgcg
     8281 ctcggcgaga agaaagtcaa gaccgtggag gacgtggccg attgcgccac cgacgatctc
     8341 ttgggctgga acgagcgcaa ggacaaggag accatccacc acgagggcat cctcgacggg
     8401 ttcaacttga gcaagcagga aatcgaggat ttgatcctcg ccgcgcgggt caaggccgga
     8461 tggatcgacg aagccgatct tgcgcctgag ccagaggctg aagaggccga ttctggcgaa
     8521 ggcgaagagg ttggcgaaac caaggacggc ggcgccgcgt catgagcggc cccagccacc
     8581 agacgctttt taggagagag agccgttgcg gcaggacgtt gctgagccac agcttgaggc
     8641 ggagagcgag acgggagaca ccgtccgccg ctgcgcgctc acgcggatgc gcctgcccaa
     8701 gcaggatctg atccgcttcg tccttgcccc aaccggcgaa atcgtccccg acctgaagga
     8761 acggctcccc ggccgcggcg tgtgggtgac cgccgaccac gcggtcgtgg ccgaggccgc
     8821 caaatgcaac gttttcgcca gggcgctgaa ggcacaagca aaggttccgc cggggctcgc
     8881 cgcccaggtc gatcggctgc tggtcgaggc ggcgctcggc gcacttgccc ttgccaataa
     8941 ggcgggcgaa gtggtgttcg gcaccgccaa gatcgaagag gccatcgcca aggccaaggt
     9001 cgcggctctc atccatgcga gcgacgccgc agaggacggc tgccgcaagc tcgacggccg
     9061 atttcgggcc gtcgcaaggg gtgagacgcc ggccccgatc cgccttttca gcgcggatga
     9121 attgggtttg gcatcaggta agacaaatgt gatacatgca gccctgatcc agggcggagc
     9181 ggctgcgaaa ttcctcgcca gcgcttcccg cgcggagcgt tatcgcaagg gcagcgccgc
     9241 agcctttgcc cagccaattg gatcggacac ggacaaggaa tgagtgaaat caacgagact
     9301 gaagacaaaa ccgcacgcgg cggccgcaaa atgacgctca atgtgcgccg gacggtggaa
     9361 tccggccatg tgcgtcagag cttcagccac gggcgctcca agtccgtcct ggtcgagaag
     9421 aagaagcgcc gggcggtcgg cgccacgcct gcggcacaac ccgtcgagga gcccaagccg
     9481 aaggtggcga cggcgccgga gcccacgcct gccaagcacg cccgcgacga cgagcagcgc
     9541 cgtcccggcg tggtcctgcg cgccctttcc gacgaggaga aggacgcgag gctgagggcc
     9601 ctgactgaat cgcggcagcg cgaggcgatc gagcgcgaga aggccaagga agaggcggcg
     9661 aagcgcgtcg tcaccgagga agtcaaggcc aaggagcacg acgagacgct cgagcgccag
     9721 aagaaggagg aggcgcggca cgaggccgaa gccgaggcca ggcgcaaagg cgaagaggtc
     9781 gcccgcaagc atctcgtcgt taaggaggag gaggaggaag ctccccgcac gcaagaggcg
     9841 cgccacaaga aagagcgcgc cgtcaaggcg cgtgccggcg aagagcgcag ggtgcgcggc
     9901 cgcctgacca tcaccaacgc gctcgacgag gagcagcgcc agcggagcct tgcctcgctg
     9961 aagcgccacc gcgagcggca gaagaagcag actatcggtc cgcaggccca gctgaagatt
    10021 cagcgcgatg tagtgatccc ggaagtcatt accatccagg agctcgccaa ccgcatggcc
    10081 gagcgaggcg tcgacgtcat caaatatctg atgaaagacg gcgagatgca caagatcacc
    10141 gatgtgatcg attccgatac cgctcagctc atcgccgaag agttcggcca tgtcgtgcgc
    10201 cgggtgtcgg aggccgacgt ggaggaaggc ttcatcggcg ccgaggatat tgccgaggac
    10261 gtggagcccc gtgcgccggt ggtgaccatc atgggccacg tcgatcacgg caagacctcg
    10321 ctgctcgacg ccatccgcgc gaccaacgtg gtgagccagg aagccggagg catcacccag
    10381 catatcggcg cctatcaggt ggtgacgccg gccggccaga agatcacctt tatcgacacg
    10441 ccaggccacg aggcgttcac cgccatgcgc caaagaggcg cccaggtgac ggatatcgtc
    10501 gtgctggtgg ttgccgccga tgacggcgtc aagccgcaga cggtggaggc catcaaccac
    10561 gccaaggcgg cgcaggtgcc gatgatcgtc gccatcaaca agatcgacaa gccaggcgcc
    10621 gacccgcagc gcgtgcgcac cgagctgctc agccacgaga tcgtggtgga gagcctcggt
    10681 ggcgatacgc tcgagatcga ggtctcggcc ttgaagaaga ccggcctcga caggctgctc
    10741 gaggccatcg cgctgcaggc cgaggtgctc gagctcaagg cgaaccccaa tcgtccggcg
    10801 gaaggcgtca tcgtcgaggc caagctcgag cgcggccgcg gcccggttgg caccgctctc
    10861 gttcagcgcg gcacgctgca tctcggcgat ctcgtcgtgg caggctcggc ttacggccgg
    10921 gtacgcgcgc tgatcaacga tctcggcgag caggtcaagg acgcaggtcc gtcagtgccg
    10981 gtcgaggtgc taggcttcga ctcggcgcct gaggccggcg atcaattcgc cgtggtggag
    11041 aacgaggccc gcgctcggga gatcaccgat taccgcctgc gcgagcggcg caagcgccag
    11101 gccgtggccg gtcagcgcgg ctctctcgag cagatgatga cccagctgaa agcggctggg
    11161 accaaggagt tcacgcttct cgtcaaaggc gacgtgcagg gctcggtgga ggccatcgtc
    11221 gccgctctcg acaagctcgg caccgacgag gtccgcgccc gcatcattca cgccggcgtc
    11281 ggcggcatca ccgaatccga cgtcggtctc gcctcgacct cgggcgccgt gatcctcggc
    11341 ttcaacgtgc gcgccaactc gcaagcccgc gaggctgccg agcgcgacgg cgtcgagatc
    11401 cgctattacg ccgtgatcta cgatctcgtc gacgaggtga agcaagccat gtccggcctg
    11461 ctcgcaccca cgcggcgcga gaccttcctc ggcaatgccg agatcctcga agtgttcacc
    11521 atctccaagg ttggcaaggt cgccggctgc cgggtcaccg aaggcaaggt cgagcgcggc
    11581 gcacaggtgc ggctcatccg cgacaacgtc gtggtgcacg agggcaagct gtcgacactc
    11641 aagcgcttca aggacgaggt gaaagaggtg gtggcaggcc aggaatgcgg catggcattc
    11701 gaaggctacc aggacatgcg ccagggcgac gtcatcgagt gcttcgaggt cgagcacgtc
    11761 cgccgcacgc tttaagaccg cttgagtcga gcggcctcag acattgccat gacggatcgc
    11821 cacagaggta aggcgccgag ccagcgccag ttgaaagtcg gcgagactat ccggcacgcg
    11881 cttgccgaga tcttcgcccg cggcgagatc gtcgacgagg tgctcgacca atattcgctc
    11941 accgtgtcag aggtgcgcat gacgccggac ctgaagcttg ccaccgcctt cgtgctgccc
    12001 ttgggcggcg aaggcgccga ggatgcggtc cagcatctcg acaagcacaa gcgcttcttg
    12061 cggggcgagc tcgccaggcg cgtgagcctg aaattcatgc ccgagatcag attcaagatc
    12121 gacatctcct ttgccagctc gaaacgcatc gatgagctgc tcgcctcgcc aaaggtcgcg
    12181 cgcgacctcg acgccgacta gtagcagcag aggcgacatg gcgcggcggc gcaaaggtct
    12241 cccagttcac ggctggctca tcttcgacaa gcctaaaggc atgacctcca cgcaagccgt
    12301 cacgcgggta cggcacctct acggtgcgca gaaggccggc catgccggca cgctcgaccc
    12361 gctcgccacc ggcgtcttgc cggttgcctt cggggaagcg accaagaccg tgcccttcgc
    12421 ggtcgacggc gacaagcagt accgcttcac cgtgcgcttc ggggaggcca ccgacaccga
    12481 cgactccgat ggcaccgtaa tagcaacgag cgaccggcgc ccgtcccgga ccgagatcga
    12541 agcggcactc cccagcttcg tcggcgagat cagccaggtg ccgccgcgct attcggcgct
    12601 caaggtggga ggcgccaggg cctacgacct tgcccgggaa gaccaggact tcgagcttgc
    12661 cccgcgccgc gccctgatcg aaagccttga gctgaccgac atgcccgacc ccgatcattg
    12721 cgtgattgag gcgcgctgcg gcaagggcac ctatgtgcgg gccttggccc gcgaccttgg
    12781 agaggccctc ggctgcttcg cccatgtcac ggaattgcgc cgaacgaggg tcgggccttt
    12841 cggcgaagag gccgcaattt ccctggcaaa gcttgaggag ttaggacata gtgccgccgg
    12901 ccgcgacgcc cttttgggcg ttcttaagcc cgtgcagacg gcgctggacg acatcccggc
    12961 gctcgccatc agcgggcagg acgcggccag gctcaagaga ggccaacccg tgctgttaag
    13021 aggccgggat gctccaattc tcaaagggcc tgtctatgcc acgtcacgag gttcgctcgt
    13081 ggcggttggt gaggtcagcc aaggcgaact gcgccccacc cgggttttca atcttcccgg
    13141 ctagggccat cgtcaatttc acaggagttc atccgatgtc gatcagcgct ttgcgcaagc
    13201 aagagcttat caaggactat gctaccacgc ccggagacac cgggtcccct gaagtgcaag
    13261 tggcgatcct gaccgaacgg atcaccaatc ttaccgatca cttcaagacg cataacaagg
    13321 ataaccattc acggcgcggg ctgttgaaaa tggttagcca gcggcgccgg ttgctcgact
    13381 acgtcaaggc gacggacgag gaccggtatc agaagcttat caaccgcctg ggcattcgtc
    13441 ggtaatgcga gacgcttacc gccgtgccgg tggacctcca cacacatatc gacccgagcc
    13501 atcgccttca gggggcccgc tcgggcgcaa ccattccctg ttcgtcgaga caacatcatg
    13561 ctgcataagc acgtcgtaga aattgaatgg gcgggccgac cgctcagatt agagaccggc
    13621 cgcatcgcgc gccaagcgga cagcgccgtt ctcgcccaat atggcggcac atcggtgctc
    13681 gccaccgcca cggcggccaa agaggccaag ccaggcatcg actttttccc gctgaccgtg
    13741 aactaccagg aaaagacctt cgccgccggc aagatccccg gcggctattt caagcgcgag
    13801 ggacggccca ccgagaagga gacgctgacc tcacgcctca tcgaccgccc gatcaggccg
    13861 ctatttgccg gcgggttcag gaacgagacc caggtggtcg ccaccgttct ttcccatgac
    13921 atggagaacg atccagacat cgtggcgctg atcgcgagct ccgccgcgct taccctttcg
    13981 ggcatacctt tcctcggccc gatcggcgcc gcgcgggtcg gctacatcgc cggcaaatat
    14041 gtgctcaatc cgatgatcga cgagatgcgc gaaagcacgc tcgatctcgt ggtcgccggc
    14101 accgcaagtg ccgtgctgat ggtcgagtcg gaagccaagg agctgccgga agatgtgatg
    14161 ctcggcgccg tgatgtacgg ccagaagcac ttccagccgg tgctcgacgc catcgtcaag
    14221 cttgccgagc gcgcggccaa ggagccgtgg gagctgaaat cagacgcgag cgccgaggag
    14281 cttgccgcca aggcgcgggc gattgctgaa gtcgagctac gcgaggccta caagatcgcc
    14341 gccaagcatg agcgccgcga cagcgtcgcc gcggtcaagg agaaggtggt caccgccttg
    14401 gcgcctgaga gcaccgatgt cacggtgatc ggcaaggcgg caagcgcctt caaggagctg
    14461 gagaaggacg tggtccgctc gagcatcctc gcaaccaagc accgcatcga cgggcgcgac
    14521 ctcaccacag tccggccgat cgtctgcgaa gtcggcatcc tgccacgcac gcacggcagc
    14581 gcgctgttca cccgcggcga gacgcaggct ctggtggtgg cgacgctcgg caccggcgag
    14641 gatgagcaat atatcgacgc gcttgagggc acctacaaag agaccttcat gctgcactac
    14701 aatttcccac cttattcggt gggcgaggtc ggacgactcg gctcgcccgg ccgccgcgaa
    14761 atcggccacg gcaagcttgc ttggcgggcg atccatccgg tgctgcctga tcgcgagact
    14821 ttcccgtaca ccatccgcgt ggtctccgag atcaccgagt cgaatggctc ctcctccatg
    14881 gccacggtgt gcggatcctc gctcgccatg atggatgcgg gcattccctt gcgccggccg
    14941 gtggccggca tcgccatggg attgatcaag gaggaggccg acttcgccgt cctctccgac
    15001 attctcggcg atgaggatca tctcggcgac atggacttca aggttgccgg cacctccgaa
    15061 ggggtgacct ccctgcagat ggacatcaag atcaccggca tcaccgagga gatcatgaag
    15121 gtcgcgctcg atcaggcgcg cgagggccgc atgcacatct tgagcgagat gaacaaggcg
    15181 ctcaacgagg cgcggcccga gcttggcgaa tatgcgccgc gcatcgagac gatccacatc
    15241 ccggtcgaca agatccgcga ggtgataggc tccggcggct cggtgatccg cgagatcgtc
    15301 gcggagtcag gtgccaagat cgacatctcc gacgacggca ccgtcaagat tgcctcggcc
    15361 aacgccgaat cgatccgcgc cgccatctcc cgcatcaagg gcatcgccgc cgagcctgag
    15421 gtcggcgaga tctatcacgg caaggtggtc aaggtgatgg agttcggcgc cttcgtgaat
    15481 ttcttcggcg ccaaggacgg gctcgtgcac atctcccagc tcgccgaagg acgcccgaag
    15541 gcagtgaccg acgtggtcaa ggaaggcgac atggtgtggg tgaagctgct cggcttcgac
    15601 gaccgcggca aggtaaggct gtcgatgaag gtggtcgatc agacgaccgg ccgtgagctg
    15661 caaaaggccg aagcaagcta aggctgagca gtcatcacaa gaagcgaatg cgtcatggcc
    15721 gggcttgtcc cggccattta cgtttcagct cttctgcttc tcgcagcgcc agccgatgac
    15781 gaaatattgg ctgttggcct tctgccattc gtcggccttg tcgcccgcag tcttgaggca
    15841 gctcaccaca tcagtcgccg tggcgccggg gaccggcttt tccgagcagt ccgtcggcgt
    15901 ggacagaaga caaatcagta cgaccagctg agccatcggc aaacttcctc caatcccttg
    15961 atatcgcggc ctttactgtg ccgcctcgcg cggcgccttt tccggcgcag ctttgagttc
    16021 cttgtcattt tccatggact tgagcgcagg gaggcccggc atggagatgg tcgagtagcc
    16081 gcaatcgacg aaatgaattt cgccggtcac cgcgccggac agatcggaca gaagatagag
    16141 cgcagcaccc cccacctcgt cgagcgtcgg cgagcgatgc atcggcgcat ggcgctgctg
    16201 atagtggaac agcgtgcgcg cgtccgagat gccggcgccg gcaagcgtgc gcatcggccc
    16261 tgccgacagc gcgttcaccc tgatcgcttg cgggccgaga tctgtggcga gataacgcac
    16321 gctcgcctcg agcgcggcct tggccacgcc catgacgttg tagcagggaa cgacgcgggt
    16381 cgagccgcca taggtaagcg tgagcagcga gccgccattc ggcatcagct cggaagcgag
    16441 cttggcgatc tcggtgaagg aaaagcacga gatcaccatg gtgtgctcga aattggcgcg
    16501 agtcgtgtcg gcgtagcgcc ccttcagctc gttgcggtcg gagaaggcaa gcgcgtgcac
    16561 gacgaaatcg agcgagcccc atttcttgcg gatcacctcg aacacgcgtt tgacgctctc
    16621 cagatcctcg acgtcggcct cttcgatgat cggggcgccg atcgattggg cgagcggcac
    16681 ggcgcggcgg ccgaaagcgc cgccctgata ggagaaggca agctcggcgc cctggccgtg
    16741 cagcacgcgg gagatgcccc aggcgatcga gcgatcattg gcgacgccca tcaccagccc
    16801 cctcttgccc ttcatcagcg gtccgggctc ggcgatatct cttgttgttg tcatggtact
    16861 tactctggtt aggggtgaag agggatgcgt ccgcttcctt gcctttagcc gttgtagcgt
    16921 cggaacacga gcgtggcgtt ggtaccgccg aagccgaagc tgttggacat gacgcagtcg
    16981 atgtcgacat tgtccttacg cttgcgcacg atcggcatgt cggccacggc gggatcgatg
    17041 gtctcgatat tggcggactc gcaaatgaat ccgttgttca tcatcaacag cgaatagatc
    17101 gcctcgtgtg ccccggcggc gccgagcgaa tggccggtga gcgacttggt ggcgctgatc
    17161 ggcggcgact tgtcgccgaa cacctcgcgg atcgcctcga tctccttgac gtcgccgatc
    17221 ggggtcgagg tggcgtgagg attgatgtaa tcgatgccgt cgacgccctg catggcgagg
    17281 cgcatgcagc gcgctgcccc ttcgccggaa ggctgcacca tgtcgtagcc gtctgaggtc
    17341 gcgccgtagc cgacgagctc gccgtaaatg cgggcgccgc gagccttggc atgctcgagc
    17401 tcctcgagga tgacaacgcc ggcgccgccg gcaatgacga agccgtcgcg ggcgacgtca
    17461 taggcgcggc tcgcgacctc cggccggtcg ttgaagtccg acgacatggc gttcatggcg
    17521 tcaaacagga cagagagcgt ccagtcgagc tcctcgccgc cgccggcgaa caggatgtcc
    17581 tgcttgcccc attggatcag ctcgccgcca ttgccgatgc aatgcgcgct cgtcgagcag
    17641 gccgacgaga tcgaatagct caagcccttg atgtgatagg gcgttgaaag cgtcgccgag
    17701 ttggtcgagg acatcgattt cggcacctcg aaggggccga ccttcttcgg gcccttctcg
    17761 cgcgtggtgt cggcggcctg gacgatggct tttgttgacg ggccgcccga gcccatgatc
    17821 aggccggtgc gctcgttcga gacatccgtc tcctcaagcc cggcatcgcg gatcgcctgc
    17881 tccatggcga ggtagttcca ggcagcaccc gctcccatga agcggcggat cttgcggtcg
    17941 atcatcccct cccaatcgag cttcggcgcg ccatgcacct ggcagcggaa gccgagctcg
    18001 gcataggtgt cggccttgac gatgccggac ttcgcctcgc gcaacgaggc gagcacctcc
    18061 tgggcgctat tgccgatcga ggacacgatg cccatgccgg tcacgaccac gcgtctcatg
    18121 acttcgtcct tagcccctta agatgatccg ggtgcctggc tcgcgacgat cacgcaccgg
    18181 cgggagcttc ttcgcccgca tggaacaggc ccacgcgcag atccttggcg gtatagatcg
    18241 tatcgccgtc ggctttaagc cagccgtcgc cggtgccaag ggtaaatttt cgccggatca
    18301 cccgcttcag ctcgacgatg tattccagct ttttcatggt cggcagcacc atgccggaga
    18361 atttgacctc gcccacgctg agtgcccggc ccttgcccgg agccccgagc catccgagga
    18421 aaaagcccaa aagctgccac agggcgtcga gcccgaggca gcccggcatc accgggtcgc
    18481 ccttgaagtg acaggggaaa aaccacaggt ccggcttcac gtcgagctcg gccacgatgc
    18541 ggccatagcc gtgctcgccg ccctcctcct tgacctcgga gatgcggtcg aacatcagca
    18601 ttggcggcag cggcagctgc gcgttgccgg ggccgaacag ctcgccccgc gcgcaagcga
    18661 gcaggtcctc gtaatcgaac tgttcgcgac gctcgaccat ccttgatcct tccatgtgcg
    18721 gggcgatcct ccccaccaga gggccgtttc ctaacatagg actcggaaat cagaaatcag
    18781 ttatcagaaa agttctggct ccaatcctag ttcccgggct ctgaattatg ccttctatcc
    18841 tcccgttgta tcgttgcagg cggcggcgct tcaacatata gtgctggcgt catgacttta
    18901 cctgcaatgg atatcgccga gcgcctgcgc gcggcggggt tgcggccgac gcgccaacgc
    18961 gttgcgcttg cgcgcctcct gttcgacggc tgcgaccgcc acgtcacggc cgagctcctg
    19021 catgaagagg cgcagagccg gaaggttccg gtgtcgctcg caacggtcta caacaccctg
    19081 catcagttca ccgaagccgg gctcttgcgc gaggtggcgg tcgagggcgc caagacctat
    19141 ttcgacacca acacctccaa ccactaccat ttcttctgcg agaattcggg cacgctctta
    19201 gacatccaga cggcttcgat ccgcatcgaa ggcctgcctc aggcgccgga cggcatgtcc
    19261 atcagccgcg tcgacgtgct cgtgcgcctg gtcgacgacc gcatgcgata attcattatt
    19321 tgctgaagaa tattcctctc cccagcggcg agaggtcgcg ctgtcgccgc ctgcggcgag
    19381 agcgcgggtg ggggttacgg agttctgaga gtcacgatcc ccctcacccc cagccctctc
    19441 ccccatgggg agagggggat tacttgatca cctcgtcttt gtagacgccc cacagcttgt
    19501 cctgctggac gaagccggaa tagtcgtcaa tcgacagctc gcaccactct ccgtcgcagg
    19561 agagcacgtt tccgagcacg ctcggcgcaa gctcggccac cgtgctcgcc cccgtcgact
    19621 ttcgcgaata gagcggcacg ttttccggcg tcttggtcca cggcgagatc agcacggtgc
    19681 gccggccgct gagcaggcta tgccacaccc agccgtcggc gccttcgctg tcgcgcacgc
    19741 gccgccaatt gtcggactcg gcgatgatct cgacggggag ccctgcccgg ctgaacaccc
    19801 acacgatggc ctgatcggtg ctcgggccct tgcgcacatt gacccggtcg gatttcaggc
    19861 tgacgaaacg cggtataggc agccgggtct ggctgctgct cgagcccgtg gcagggcttg
    19921 cgtcattggc ggcccgggtc tcgatgccgg caaggaagag aatcgcaagc gcgacccctg
    19981 ccgccagac
//
LOCUS       DATNAE010000193        11355 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_1995605, whole genome shotgun sequence.
ACCESSION   DATNAE010000193 DATNAE010000000
VERSION     DATNAE010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 11355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11355
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1995605"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /locus_tag="VN518_04320"
     CDS             complement(<1..930)
                     /locus_tag="VN518_04320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HXE03832.1"
                     /translation="MVAVLVLDGVAYLLAVNTVADGHHELVSVLADIFVLVALLTISV
                     GLIPPGMITYSATEVSKAAKRLTSGTLRQFSQAMGALGRGDLEAAHASVNIVPVKPNS
                     RDELGEMAESFNVLQKEVREAALGLGEAREKLRAARAELLARHRYIAHLAHHDSLTNL
                     PNRTALAEQVAQTLAEAKKNGTSFAFLTVDLDHFKEANDLFGHVIGDELLCAIARRLQ
                     HAADDAFIARMGGDEFTLILATGEQPRAAQALANRLLNAVAKPFEVRGQQVPIGLSVG
                     GTIYPQDASDIDGLIANADAALFRAKADGRHMAR"
     gene            complement(1075..2127)
                     /locus_tag="VN518_04325"
                     /pseudo
     CDS             complement(1075..2127)
                     /locus_tag="VN518_04325"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
     gene            complement(2155..2358)
                     /locus_tag="VN518_04330"
     CDS             complement(2155..2358)
                     /locus_tag="VN518_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03833.1"
                     /translation="MESTTLQQDPVTPPHPRVLGWVGTAALAMGGSNQMIFLITALFV
                     GQGVILGRGSAAVPLLIVGVLLG"
     gene            complement(2534..2809)
                     /locus_tag="VN518_04335"
     CDS             complement(2534..2809)
                     /locus_tag="VN518_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009734198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03834.1"
                     /translation="MSNFDYTAEAELFPARNWRAKSRGVGYKRFTRASEAIRFAMEEL
                     APESLLGAYLEVEEERFDGSGIRRLYESRDYPLARRVAPQGGRRKTG"
     gene            complement(3061..4065)
                     /locus_tag="VN518_04340"
     CDS             complement(3061..4065)
                     /locus_tag="VN518_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD regulator"
                     /protein_id="HXE03835.1"
                     /translation="MAQALRAVEETVGGTQIELGLNAAIVSVREQQPQILVVRPGEVA
                     SSEWDALPFGPFAPRAHRTLEIGLRAWVKQQTGLDLGYVEQLYTFGDRGRHAEPGDTA
                     SHILSVGYLALTRGAGEELHGSHWSPWYAYFPWEDWRQGKPEIFTNEIEPRLKEWAER
                     SPSPDEPARPLRRSERLKVGFGIGGGFDEEKVLERYELLYEAGLVTEAARDGRSAARQ
                     WKDLPELGRPMEFDHRRILATAMGRLRGKLKYRPVVFELLPPEFTLLDLQKTVEAISG
                     TLLHKQNFRRLVEQGGLVETTGNVSTETGGRPAKLYRFRREVVLERPAPGLRVKAGRA
                     "
     gene            complement(4069..4596)
                     /locus_tag="VN518_04345"
     CDS             complement(4069..4596)
                     /locus_tag="VN518_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019963069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HXE03836.1"
                     /translation="MSPDTIFVSPLSLVETTIEDAQVSHLVTLINGETLVPTPPSITP
                     DRHLRLDMNDICEPIMGLVLPCEEHVADLVQFALDWDRRAPLLIHCWAGISRSTAAAF
                     ISLCALNPDADELGLARALREASRTAYPNRRLVALGDEVLSRQGRMIAAVERIGRGEI
                     AEEGQVFGLSARNAA"
     gene            complement(4680..5294)
                     /locus_tag="VN518_04350"
     CDS             complement(4680..5294)
                     /locus_tag="VN518_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD family hydrolase"
                     /protein_id="HXE03837.1"
                     /translation="MKSLPAEAGARAWQRMLSGRRLDLLNPSPLDIEIEDIAHGLARV
                     ARWNGQTTGDHAFSVAEHCLLVETIAGALSPGLSRSYRLAALMHDAPEYVIGDLISPF
                     KAALSLDYKAFEARLLAAIHIRFGLPGELPAEISKLIKRADKIAAYYEATALAGFSVE
                     EGRRYFGQPRGLKPGLVAKLTRLTPLPAADSEAKFLKRFNELAQ"
     gene            5417..5659
                     /locus_tag="VN518_04355"
     CDS             5417..5659
                     /locus_tag="VN518_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008125023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HXE03838.1"
                     /translation="MAKLPRFSLAHNEQTKKWELKTEGSSEVVKKFPTKAAALKGGVL
                     EKAVGGRGSVRIRKRDGKIQEERTYPRSADPRGSKG"
     gene            complement(5649..6245)
                     /locus_tag="VN518_04360"
     CDS             complement(5649..6245)
                     /locus_tag="VN518_04360"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HXE03839.1"
                     /translation="MSKSEAKRCPWSGSDVLYIAYHDEEWGVPKSDARALFEKLVLEG
                     FQAGLSWITILRKREAFRAAFDNFDAQAMARYGPRKVESLLKDEGIVRHRGKIEAAIS
                     NARAFLELESGPGFSQFLWSYVDGTPKQNAYKRMADLPAETPESKALAKALKSHGFRF
                     TGPTTMHAFMQGVGMVNDHLTGCFRHEECAKLAARFSL"
     gene            complement(6242..7120)
                     /locus_tag="VN518_04365"
     CDS             complement(6242..7120)
                     /locus_tag="VN518_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018043422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HXE03840.1"
                     /translation="MTMKKCNAALLSDRAVVRVSGPAATSFLQGLVTNDIDKAKPGGA
                     VHAGLLTPQGKILFDFFVVPAGEGFLLEIAKAKAAELVQRLGFYRLRAEVEIAEGPAF
                     TVAAAWGEPARLPDGALAHADPRLPALGERILLPAGTSLGNLGCTPAGEDEYHAHRIA
                     LGVPEGGRDYAFGDAFPHEAMFDQLAGVDFNKGCFVGQEVVSRMEHRGTARKRIVGVE
                     GEGPLPPSGTDITAGGASIGTLGSVSGSSGLALLRLDRAEEAKAAGVPLRAGEVTVAL
                     RIPAWARLKTPAAAAS"
     gene            7178..8521
                     /locus_tag="VN518_04370"
     CDS             7178..8521
                     /locus_tag="VN518_04370"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HXE03841.1"
                     /translation="MPETFDLLIKGATLVNHAGEGLADIGVKDSRIVAIGSYAGHRAG
                     RTIDAAGLHVLPGVIDTQVHFREPGLEHKEDLQSGSRSAVLGGVSAVFEMPNTKPATV
                     SAGALADKVARGRGRMYCDFAFFVGATRENVEELPRLERLPGAAGIKVFMGSSTGDLL
                     VDDEETLARILALIERRAAFHAEDEARLRERASLRRYGDPASHSEWRDPEAALAATAK
                     LLRLATAAGKRVHVLHVSTAAEMQLLALHKDVASVEVTPQHLTLAAPEIYRRLGTKAQ
                     MNPPLRDAAHQNVLWWGLNQGVVDVLGSDHAPHTREEKAQDYPASPSGMPGVQTLVPV
                     MLDHVNKGRLSLARFVDLTSAGPQRLFGIAGKGRIAAGYDADLTIVDLKAERVIEEDW
                     IGSKCGWTPFAGEKVKGWPVGTIVRGTLAMWDGELGAASGEPVRFGEALPRDPLA"
     gene            complement(8526..9260)
                     /locus_tag="VN518_04375"
     CDS             complement(8526..9260)
                     /locus_tag="VN518_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03842.1"
                     /translation="MSRTKIVMAAFAALVAGTLLIVYAQWGEHASRAAVQSGQEVSPA
                     SDDERPQAIAASEAGEPEAAPAPKPESDAPKSESATPPAPPGAEAPPPGTPEVIYDFN
                     RLPDEIRDTLQSIVVAAQSGNIDEMLPVLEENELPPMLSATAVSDPIAFWKKASADGQ
                     GHDILAAVMNVFSSGFMRKGEGKDVMYIWPYFAEMDLTKLTPAQEVDFYRVVPPPLGV
                     EMKKSGKYTYYRAGIGADGVWHYFLR"
     gene            9374..10042
                     /locus_tag="VN518_04380"
     CDS             9374..10042
                     /locus_tag="VN518_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HXE03843.1"
                     /translation="MPSGAHIVLSGIQLVLAGIGVGLLVAAPIGPVNVLCIQRAVTNG
                     FWGGLAAGIGAMLGDGLLAAIAAFSVTAISDVLIAYSGLIQLVGGVLLIIFGIGLLFA
                     PPMLLIAPGQKSHLWDHWGIIPQTFFLTISNPGAILGMLALIGGLGSLIGGLDTYLEA
                     LLLVVSVMGGSLLWWLGLSELIATIRHRLTESRLRLINRIAGTLLIAFGVVLLVNELV
                     LGWG"
     gene            complement(10049..10306)
                     /locus_tag="VN518_04385"
     CDS             complement(10049..10306)
                     /locus_tag="VN518_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006933467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03844.1"
                     /translation="MQPAERAGETAEDKARSQEALRYILDAWEEALHDGIEPEMLANA
                     ALFAALADLVAAYGEDAVAKMTGGLSRRIQHGEFTLNRTPQ"
     gene            complement(10442..11242)
                     /locus_tag="VN518_04390"
     CDS             complement(10442..11242)
                     /locus_tag="VN518_04390"
                     /inference="COORDINATES: protein motif:HMM:TIGR02301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02301 family protein"
                     /protein_id="HXE03845.1"
                     /translation="MLAVAMLGWAPVAEAQFFWSPFFQQEQPKRQKVRPAKPKTPADD
                     LPPIIPRESSTPPPPDDRPYDAKLLRLAEILGAVHYLRELCGAQEGQLWRDQMKEIVR
                     NEGSTAVRRAKLVNSFNDGYRGYRRTYRSCTSSATLAITRFSVEGAQIAAALAKPSHY
                     ASRGSSDATSGSTTTATDAAPAPATGNAPAAANSAAPTPEAKPAEAAKPAANQTVATP
                     QTQASQGQAVTPDTGQVDGVEKPKRKKRKAAKAQPEETQQSIPFFGRR"
BASE COUNT         2043 a         3589 c         3728 g         1995 t
ORIGIN      
        1 ccgcgccatg tgccgtccgt cggccttggc gcggaacagc gcggcgtcgg cattagcgat
       61 gagtccatcg atatcggagg cgtcttgggg atagatcgtg ccgccgacgc tgagcccaat
      121 ggggacttgc tggccgcgta cctcgaacgg ctttgccacg gcattgagca gtcgattggc
      181 caaagcttga gcggccctgg gctgctcacc ggtcgccagg atgagggtga attcgtcgcc
      241 gcccatccgg gcaatgaagg cgtcgtcggc ggcatgttgc aggcgccgcg caatggcgca
      301 gagcagctcg tcgccgatga cgtggccgaa caggtcgttg gcctccttga agtgatcgag
      361 gtcgaccgtc aaaaacgcaa agctcgtgcc gttctttttg gcctcggcca aagtctgagc
      421 gacctgctcg gcaagggcgg tgcgattggg cagattggtg agcgagtcat gatgggcgag
      481 atgggcaatg tagcggtgcc tggcgaggag ctcggctcgc gccgcgcgta acttttcgcg
      541 cgcctcgccg aggccgagcg ccgcttcccg cacctctttc tgcaggacgt tgaagctctc
      601 ggccatctcg ccgagctcgt cacgggagtt cggcttgacc ggcacgatgt tgaccgatgc
      661 gtgggcagcc tcgagatcgc cgcggcccaa ggctcccatg gcttgcgaaa actgccgcaa
      721 ggttcccgag gtcaagcgct tggccgcctt gctgacctcg gtcgccgaat aggtgatcat
      781 gcccggcggg atgaggccca cgctgatggt cagcaaggcc acgaggacga aaatatcggc
      841 cagcaccgac acgagctcgt ggtgcccgtc ggcaactgtg ttgaccgcga gcagataggc
      901 gactccatcc agcacgagca cggccaccat ggcgccggtg agcttgacgt ggagcgtctg
      961 ggccacggcg ggaaggccgg ctctccgccg atcctcgatg acgcgccagg cgtagagagc
     1021 cgcccccgaa taagccaagg cgaggccgaa cacgaccgtc gggagaccga attgctggaa
     1081 gccgagaacg gcagcgagca gccaaatgca ggcggcgacc acaccaagca tgatggtgcc
     1141 gcgcggcgcg cgatagggac gctcggcgtc gggcaggtcg cggcggagca gccaagccgc
     1201 gatgttcggc atgcaaatgc cgatgaggta ggtgaagttg gcggcggcga tcatccatac
     1261 cggatcgccg gccaacagga agatgatggc ggagccggcg gtcagcgaag tcgccgtcca
     1321 cgggcagtcg gtcgccgcaa tgcgccacga caggaagcgg ggaaggagcc cgtcgtcgct
     1381 gagctgagac agcgtgcgag cggcgccggc gagcggctgc agcgtgccgt ggaacatgtt
     1441 gaacatgatg aaccagatgg ccgcggcctt gccgaagctg ccgaagagcg gcgcgaaagt
     1501 ggccccgagc accatggcga ggtcgtgccc gagcggctcg gggccaagca ccccgagcca
     1561 gaccaccgga agaacgatga aatacacccc tgccatggcg ccggcggcga gcatggcacg
     1621 aggcacgttg cggttcggat caacggtctc gccgacgtgg caggcggctg cctcgaaggc
     1681 cggggcggca aacccgatca gatagagacc ggccatgatc gaggtgagat tgccgaacca
     1741 gccttcgaag ggcgtggtca aatgatagtc gaaggcttca gtcggtttta ggtcgccgcc
     1801 gataattgga gcgagcgccg agacgaaagc gagcgacgcc gacgccgtgg ctatggggat
     1861 ggcgaggcga ccgacccatt taatgccgca cagattcacg aaactgaaaa agcagacgat
     1921 ggtgatggcg acagcttcga ccggaagccc ggggaagtac caatgctgga aggctgaggc
     1981 agagagcaga gcggtcaagc cgcaggtctg cacccagccc caccagtagc acgtgccggt
     2041 cagcgccgcg agcaccgggt tgtaaggccg gaaggcttcg gcgcaagccg ccgagattcc
     2101 gccgacccgg ttcggccaca tcaggacgag ctcggtccag gcaggtgccg cggcctaacc
     2161 gagcagaact ccgacaatga gcaacgggac ggcggcgctg cctcgcccga ggatcactcc
     2221 ctgaccgacg aacagcgccg tgatcaggaa gatcatctgg ttgctgccgc ccatggcgag
     2281 cgcggcagtg ccgacccagc caagcactct tggatggggc ggcgtcactg gatcttgctg
     2341 gagagtagtg gactccatcg gcgacccttc ccgttcgcgt gtaggagcca cgagccagga
     2401 acaccacacg aacacgcggc aatggttacc gaggcctcca tttcatgccg gcctgcggag
     2461 gcctcggtcg gttctgccga cgcagctcaa taagccaaga cggcccttaa gaggtctccc
     2521 tcagatttac ggctcaccca gttttccgtc tgccgccctg cggcgcaacg cggcgcgcaa
     2581 ggggatagtc gcggctctca tacaagcggc gaattccgct gccatcgaac ctctcctcct
     2641 caacctcaag ataggcgcct aagagggact caggagcaag ctcttccatg gcgaagcgaa
     2701 tggcttcaga ggcgcgggtg aagcgcttat agccaacgcc gcgggatttg gctcgccagt
     2761 ttctggcggg gaacagctca gcctctgcgg tgtagtcgaa attgctcatg atgcgaaacc
     2821 ttacgaacgc tggtttgccg aattgcgatg acggcaaaac gtcggtggga agctacatcc
     2881 aatgccccaa actaaaaggg caatatcatc tgaatgttga ttagccaaac tacgggttga
     2941 atgtggttct gaatcggcga aaatacaagg gatttggcca aaaaatgacg cggcaccgcc
     3001 aagtttcatt tggcaattgc ttcgcctgga ttcctggagt tcttcgctga gcctctagac
     3061 ttacgcgcgc ccggccttga cgcgcagtcc cggcgcaggc cgctcaagca ccacctcgcg
     3121 gcggaagcga tagagcttcg ccgggcgccc tccggtctcg gtcgagacgt tgccagtggt
     3181 ctccaccaac ccgccttgct cgaccaggcg gcggaagttc tgcttgtgca gcagcgtgcc
     3241 cgagatcgcc tctaccgtct tttgcagatc gagcagggta aattccggcg gcaacagctc
     3301 gaacaccacc ggccggtatt tgagcttgcc gcgcaggcga cccatggcgg tagcaagaat
     3361 gcggcgatgg tcgaattcca ttggccggcc aagctcgggg agatccttcc attgacgcgc
     3421 ggccgagcgg ccgtcgcgcg cggcctcggt gacgagcccg gcctcgtaga gcagctcgta
     3481 gcgctccagc accttctcct cgtcgaaacc tccgccgatg ccgaagccaa ccttcagccg
     3541 ctcggaacgg cgcagcggtc ttgccggctc atccggcgat ggcgagcgct cggcccattc
     3601 cttcagccgc ggctcgatct cgttggtgaa aatctcgggc ttgccttgcc gccaatcctc
     3661 ccaggggaaa taagcgtacc agggcgacca atgggagccg tgcagctctt cgccggcgcc
     3721 gcgcgtcagg gcaagatagc cgaccgagag aatatgcgag gcggtgtcgc ccggctccgc
     3781 gtggcggccg cgatcgccga aagtgtagag ctgctcgaca tagccgaggt cgagcccggt
     3841 ctgctgcttc acccaggcgc gcaagccgat ttcgagggtg cggtgggcac gcggggcgaa
     3901 cgggccgaag ggaagcgcgt cccattcgct cgacgccact tcgccggggc gcaccacgag
     3961 aatttgcggc tgctgctcgc gcacgctgac gatggcggca ttgagcccaa gctcgatctg
     4021 ggtgccgccg acggtctctt ctacggcgcg aagggcctga gccatggctc aagccgcatt
     4081 ccgggcagaa agtccgaaca cctgcccttc ctcggcaatc tcgccgcggc cgattctctc
     4141 cacggcggcg atcatgcgcc cctgacgcga aagcacctca tcgccaagcg cgacaagcct
     4201 gcggttggga taggcggtgc gagaggcctc gcgcaacgct ctcgccaagc ccagctcgtc
     4261 ggcgtccgga ttgagcgcgc aaagcgagat gaaggccgcg gcggtggagc ggctgatgcc
     4321 ggcccagcaa tggatgagga gcggcgccct gcggtcccaa tcgagcgcga actggacgag
     4381 atcggcgacg tgctcctcgc aaggcagaac gaggcccatg atcggctcgc agatgtcgtt
     4441 catgtcgagc ctgagatgcc ggtcgggcgt gatcgaaggc ggcgtcggca ccaaggtctc
     4501 gccgttgatc agcgtgacaa ggtgactcac ctgggcatct tcgatggtgg tctcgaccag
     4561 actcaagggc gagacaaaga tggtgtcggg cgacatgatt cgcggcacgc taggggtgtc
     4621 tcttggcgaa atcagggagg atgtttgacc atcctcgtta aggctgctct gcatcacctt
     4681 cattgagcaa gctcgttgaa acgtttgaga aatttcgctt cgctgtcggc cgctggcagc
     4741 ggcgttaacc gtgtcagctt ggccaccaac ccgggcttga ggccgcgcgg ctgaccgaaa
     4801 tatctccgcc cctcctcgac cgaaaatccg gcaagtgcgg ttgcttcata atacgccgcg
     4861 attttgtcag cacgtttaat cagcttggat atttcggcgg gaagctcgcc cggaaggccg
     4921 aagcggatat ggatggcggc gagcagcctc gcctcgaagg ccttgtagtc gagagacagc
     4981 gccgccttga acgggctgat caagtcgccg atcacatatt caggggcatc gtgcatcaaa
     5041 gctgcgagcc ggtagctgcg ggaaagcccc ggcgacaggg cgccggcgat agtctcgacg
     5101 agaaggcagt gctcggccac cgagaaggca tggtcgccgg tggtctggcc attccagcgg
     5161 gcgaccctgg cgagcccatg ggcaatgtcc tcgatctcga tatcgagggg cgacgggttg
     5221 agcagatcga gccggcgccc gctcagcatg cgctgccagg cgcgcgcccc ggcttcggcg
     5281 ggaaggcttt tcatgccctc atgaatagct ctgctgccga ccgaggaaaa catagacgaa
     5341 accgatgcgt agtggcattc attagccgct ttttcccgcg taaggaccaa tcagcattct
     5401 catctaggag accggcatgg caaagctgcc cagattctcg ctagcgcaca atgagcagac
     5461 caagaagtgg gaactcaaga cggaaggttc cagtgaggtc gtgaagaagt tcccgaccaa
     5521 agccgcggcg ctcaagggcg gggtcctgga gaaagccgtc ggaggcaggg gctcggtgag
     5581 gatccgcaag cgggacggca agatccaaga agagcgcacc tatccgcgca gcgccgatcc
     5641 gcgcggctct aaaggctgaa ccgcgctgca agcttggcgc attcttcgtg gcggaagcag
     5701 ccggtcaggt ggtcgttgac catgccgacg ccttgcatga aggcgtgcat cgtcgtcggc
     5761 ccggtgaagc ggaagccgtg gctctttaga gctttggcaa gtgccttgct ctccggagtc
     5821 tcggccggaa ggtcggccat tctcttgtag gcgttctgct tcggggtgcc gtcgacgtag
     5881 ctccacagga actggctgaa gcccggtccc gattccagct cgaggaaggc ccgcgcattt
     5941 gaaatcgcgg cttcgatctt gccgcgatgc cgcacgatgc cctcgtcctt gagcaggctc
     6001 tcgaccttcc ttggtccata gcgcgccatc gcttgcgcat cgaaattgtc gaaggcggcg
     6061 cggaaggcct cgcgcttgcg caagatggtg atccaggaaa gcccggcctg gaatccttcc
     6121 agcacgagct tctcgaagag agcgcgggcg tctgatttcg gtacgcccca ttcctcgtcg
     6181 tgataggcga tgtagagcac gtcgctccct gaccacgggc atcgtttcgc ttccgacttg
     6241 ctcatgaggc tgccgccgcg ggagtcttga gacgcgccca ggcaggaatg cgcaaggcaa
     6301 cggtcacttc gccggcgcgc aaaggtacgc cggcggcttt cgcctcctcg gcgcggtcga
     6361 gacgcaaaag cgcaagcccc gagctgccgc tgaccgagcc gagcgtgccg atcgatgcgc
     6421 cgcctgcggt gatgtcggtg cctgaaggcg gcagaggccc ctccccctcc acgccgacga
     6481 tgcgtttccg cgccgtgccg cgatgctcca tgcgcgacac gacctcctga ccgacgaagc
     6541 agcctttgtt gaaatcgacg cctgcgagct gatcgaacat cgcctcatgc ggaaaggcat
     6601 cgccaaaggc gtagtcgcgc ccgccttcgg gcacgccaag cgcgatgcgg tgcgcgtgat
     6661 attcgtcctc gccagcgggc gtgcagccga gattgccgag actcgtgccg gcgggaagca
     6721 ggatgcgctc cccaagcgcc ggcagccgcg ggtcggcatg agcgagtgcg ccgtcgggaa
     6781 gcctggccgg ctcgccccag gccgcggcta ccgtgaaggc cggaccctcg gcgatctcga
     6841 cctcggcgcg gagtctgtag aagccgagcc gctgcacgag ctcggcggcc ttagccttgg
     6901 ctatttcgag caggaagccc tcccctgccg gcaccacgaa aaaatcgaac aggatcttcc
     6961 cctgcggcgt gagcaggccg gcatggacgg cgccgccagg cttcgccttg tcgatatcgt
     7021 tggtgaccag cccctgcaaa aagctggtgg cggccggccc tgagacccgc acgaccgccc
     7081 ggtcggaaag aagcgccgcg ttgcatttct tcatggtcat gctctagaga cttattcaga
     7141 cggacgtaag gccgcaagct tcagcgaaag cctcatcttg cccgaaacct tcgatctcct
     7201 catcaagggc gcgaccctgg tcaatcatgc cggcgaaggc cttgccgata tcggcgtgaa
     7261 ggacagccgc atcgtggcga tcggcagcta cgccggccac cgcgccggca ggaccatcga
     7321 cgccgccggc ctgcacgttc tgcccggcgt catcgacacg caggtgcatt tccgcgagcc
     7381 tggcctcgag cacaaggaag atctccagtc gggcagccgc tcggcggtgc tcggcggcgt
     7441 cagcgccgtg ttcgagatgc cgaatacgaa gccggcaacg gtgagcgcag gggcacttgc
     7501 cgacaaggtc gcgcgcgggc ggggacgcat gtattgcgac ttcgcctttt tcgtcggcgc
     7561 gacgcgggag aatgtcgagg agcttccacg cctggaaagg cttccaggcg ccgccggcat
     7621 caaggtgttc atgggctctt ccaccggaga cctgctcgtc gacgacgagg agacgcttgc
     7681 ccgcatcctt gctctcatcg agcggcgcgc cgcctttcac gccgaagacg aagcgcgctt
     7741 gcgcgagcgg gcatcactcc gccgctacgg cgacccggca agccacagcg aatggcgcga
     7801 cccagaggcc gctctcgccg cgaccgctaa gctgttgcgg ctcgccaccg cggcgggcaa
     7861 gcgcgtgcac gtgctgcatg tgtcgacggc ggcggaaatg cagctgcttg cgctgcacaa
     7921 ggatgtggcg agcgtcgagg tgacgccgca gcatctcacc cttgccgcgc cggaaatcta
     7981 tcgcaggctc ggcaccaagg cacagatgaa cccgccgctt cgcgacgccg cccatcagaa
     8041 tgtcctgtgg tgggggctca accagggcgt ggtcgacgtg ctcggctccg accacgcgcc
     8101 gcatacgcgc gaagaaaagg cgcaagatta tccggcgtca ccctcaggca tgccaggcgt
     8161 acagaccctc gtgccggtga tgctcgacca cgtcaacaag gggcggctca gcctcgcgcg
     8221 tttcgtcgat ctcaccagcg caggacctca gcgcctgttc ggcattgccg gcaaaggccg
     8281 cattgccgcg ggctacgacg ctgatctcac catcgtcgat ctcaaagccg agcgcgtgat
     8341 cgaagaagac tggatcggca gcaaatgcgg ctggactccc ttcgccgggg agaaagtcaa
     8401 aggctggccg gtcggcacca tcgtgcgcgg aaccctcgcc atgtgggacg gcgagcttgg
     8461 ggcagcttcc ggcgagcccg tccgcttcgg cgaagccttg ccgcgcgatc ccctcgccta
     8521 aaccgttagc gcaggaaata gtgccagacg ccgtcggcgc cgatgccggc gcggtaatag
     8581 gtgtatttgc cgcttttctt catctcgacg ccgagcggcg gcggcaccac gcggtagaaa
     8641 tcgacctctt gcgccggcgt gagcttggtc aggtccatct cggcgaaata aggccagata
     8701 tacatcacgt ccttgccctc gcctttgcgc atgaagcccg agctgaacac gttcatcacg
     8761 gccgcgagaa tatcgtggcc ctggccgtcc gctgaagcct tcttccaaaa ggcgatcggg
     8821 tcgctcaccg cggtcgcgct cagcatcggc ggcagctcgt tctcctcgag caccggcagc
     8881 atctcgtcga tattgccgct ctgcgccgca accacgatgc tctgcaacgt gtccctgatc
     8941 tcatcaggca gccggttgaa gtcgtagatc acttcaggag ttcctggtgg aggcgcctca
     9001 gcgccaggcg gcgcgggcgg cgtcgcgctt tcggacttcg gcgcatcgct ttccggcttt
     9061 ggcgccgggg cggcttcagg ctctcctgcc tcgctcgcag caatcgcctg cggcctttcg
     9121 tcgtcgctgg ctggcgagac ttcctggccg ctttggaccg cggcgcgaga tgcgtgttct
     9181 ccccactggg catagacaat gaggagcgtg ccagctacca atgctgcgaa ggccgccatg
     9241 acgatttttg tgcgactcat cccacctccc tcaggcgctt cgccactttc gcacagcggc
     9301 gggcgcctat cctacgcgca gggcgccaag ctctcgcgac tatccttccc catcgaccag
     9361 cagcgatagg cacttgccgt ccggcgcgca tatcgtcctg tccggcatcc aattggtgct
     9421 agccggaatt ggcgttgggc tgctggtagc ggcgcccatc ggaccggtca atgtcctttg
     9481 catccagcgg gccgtaacca acggattttg gggcggtctt gccgcaggta tcggcgccat
     9541 gctcggcgac gggctgcttg cggcgatcgc cgccttcagc gtgacggcga tctccgacgt
     9601 gttgattgcc tactccggat tgatccagct cgttggcggc gtgctgctga tcatcttcgg
     9661 catcggtctc ttgttcgcgc cgccgatgct gctgatcgca ccggggcaaa aatcccacct
     9721 ttgggatcat tggggcatca ttccccagac cttctttctc accatctcaa atccaggcgc
     9781 cattctcgga atgctcgcgc tgatcggagg cctcggttcg ctgatcggcg gcctcgacac
     9841 ttatctcgag gcgctgttgc tcgtcgtgtc ggtcatgggc ggaagcctgt tgtggtggct
     9901 cgggctgtcg gaactgatcg ccaccatcag gcaccggctg accgagagca ggctcaggct
     9961 catcaaccgc atcgccggca cgctgctgat cgccttcggc gtcgtgctcc tggtcaatga
    10021 gctggtgttg ggctggggct aaagccggct attgcggggt ccggttcagc gtgaactcgc
    10081 cgtgctgaat gcggcgggag agcccgccgg tcattttggc cacggcgtcc tcgccgtaag
    10141 ccgcaacgag atcggcgagt gccgcgaaca gcgcggcgtt ggcaagcatc tccggctcaa
    10201 tgccgtcatg cagcgcctct tcccacgcat cgaggatgta tcggagagcc tcttggctcc
    10261 gagccttgtc ctctgcggtc tctcctgcac gctctgcggg ctgcaacggg gtatcccctg
    10321 ttcttcgcgg tcccttgtgt ttaagccaaa aactggcacg gacgcgcggc ctacggggga
    10381 aaaaggcgaa ctaacggtta atcaggggtt tcgacgcgct tctcggggga cgccgagctt
    10441 gctaccggcg gccgaagaac ggaatgcttt gctgggtttc ctcgggctgc gccttggcag
    10501 ccttgcgctt cttccgcttg ggtttctcca cgccgtcgac ttgaccggtg tcgggagtaa
    10561 cagcctggcc ctggctggct tgggtctgag gcgtcgccac agtttgatta gccgcgggct
    10621 tggcggcctc cgcaggcttt gcctctgggg ttggcgcagc gctgttagcc gctgccggcg
    10681 cgttgccggt ggcgggtgca ggtgctgcgt cggtggcggt cgttgtcgag cccgaggtcg
    10741 cgtcgctcga accgcggctt gcgtaatgcg acggcttggc gagggccgca gcgatttgcg
    10801 cgccctcgac cgagaagcgg gtaatcgcga gcgtggccga ggaggtgcag ctgcggtagg
    10861 tgcggcgata gccgcggtag ccgtcattga agctgtttac gagcttggcg cggcgcaccg
    10921 cggtcgagcc ttcgttcctg acgatctcct tcatctgatc tcgccagagc tggccttcct
    10981 gggcgccgca cagctcgcgc agatagtgga cggcgccgag gatctcggcg agccgcagca
    11041 gcttcgcgtc atagggcctg tcgtcaggcg ggggcggcgt cgagctctcc cgcggaatga
    11101 tcgggggcag atcgtctgcc ggggtcttcg gcttggctgg gcggaccttc tggcgcttcg
    11161 gctgctcttg ctggaagaac ggcgaccaga agaactgtgc ctcggccacc ggcgcccagc
    11221 cgagcatcgc aacagcgagc acgcagaccc cgaatccaag cgcgggcgcc cttcgtttca
    11281 tgcaatctga gtaacgcgct ttggcggcga aagctaggcg ccgggctcgg ctcgtggccg
    11341 gcggagaatg cgcca
//
LOCUS       DATNAE010000194         6414 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2900063, whole genome shotgun sequence.
ACCESSION   DATNAE010000194 DATNAE010000000
VERSION     DATNAE010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6414
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2900063"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2000)
                     /locus_tag="VN518_04395"
     CDS             complement(<1..2000)
                     /locus_tag="VN518_04395"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXE03846.1"
                     /translation="MGGKRCAGLGLLLAPILLLFALHASAEDQQAPRAQQGLAPTIIL
                     LDGSISMWARLGGQSKVGIVRSTLTQAFTDYEDRIAFGLVVFGHRGGGACTEAELLAK
                     PGELSAKSPGKLLFGAGFKPKATKPVAAALVDAAKQTPATGLDVVLITDGPDTCKANV
                     CATVKSLKQAAPGLRIHVIGFDPKAKQTVKALSCVAKTTGGQFLTAANANDLKQDLNA
                     VLDAASKSAPRPVPVASKSPAAPPAPPAAAPPPPRADTATPGPASATPHANAAPSAAQ
                     PNPPAQAAPTAPAAQKSASANPSAPTAPAETAQAKSAQPKGPQAPQSSAVIGPQAPAQ
                     TDMAKLAAIPPKESAAPARTPAAPGTQGAEQSAPVPVTFKALLTEGGPQVKTGLIWRV
                     FTPQPGANGARKLVSTHHEAMPTAALVPGEYLVNAAYGLSNLTKKIKVESGRSLEETF
                     VLNTGGLKLAAVLAGGAALPASSVHFDILSDEEDQFGNRHKILDDAKPGVVIRLNAGA
                     YHLVSTYGDANGTVRADVTVEPGKITEATVKHAAAPVTFKLVQSAGGEALADTKWSIL
                     TTTGDVVKENTGALPTHILALGDYAVVANHNGESYTSKFSVAAGEAKQIEVVMESGPA
                     SPEALKAITEPPPPPPAPPAGTTVASPGSPDAMPGTPSMSGAP"
     gene            2195..2767
                     /locus_tag="VN518_04400"
     CDS             2195..2767
                     /locus_tag="VN518_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HXE03847.1"
                     /translation="MTNPIIAHLLTRRSVSANSLGEPGPSQDEIEQILTAAARVPDHK
                     KLVPWRFLLFQGEARAAFGEILAEICKREEKDPSQIRLETEANRFMRAPLVIAAISRV
                     VNNPAAPEWEQVLSAGAACQNLLIAATALGYGAQWITEWYGYSQGVRAALMLADNERV
                     AGFVYIGTAKEKPEERERPSLADIAQAWRR"
     gene            complement(2777..3547)
                     /locus_tag="VN518_04405"
     CDS             complement(2777..3547)
                     /locus_tag="VN518_04405"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HXE03848.1"
                     /translation="MSKFRNIAFVASEMKEAQGARQDLVARYGDTAPEEADVIVALGG
                     DGQMLQTMHRYINNRIPIYGMNRGSVGFLLNDYREDELLERLDKAEVNVIHPLRMTAY
                     NRDKAEHKALAINEVSLFRQIYQAAKLRISVDGRVRLDELICDGVLLATPAGSTAYNL
                     SAHGPILPINAPLMVLTPISPFRPRRWRGAILPNEAVVLIEVLEPDKRPVSAVADNTE
                     FRDVLAVEIREASDVDIFMMFDPGHSLAERILREQFDY"
     gene            3654..3989
                     /gene="cutA"
                     /locus_tag="VN518_04410"
     CDS             3654..3989
                     /gene="cutA"
                     /locus_tag="VN518_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HXE03849.1"
                     /translation="MTKDDRPVLVYTTFATLDDAKRVGDALVAARLAACVNIFPGMIS
                     IYEWKGAREIADEVAMIIKTRASLADAVLAETKRLHPYELPALLVLPTEGGSEEYCGW
                     ILSQTGRAP"
     gene            3986..5485
                     /gene="glpK"
                     /locus_tag="VN518_04415"
     CDS             3986..5485
                     /gene="glpK"
                     /locus_tag="VN518_04415"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018854682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HXE03850.1"
                     /translation="MSERRLVLAIDQGTTSTRAIAFDQEARVRAAAQVPLTQIYPAPG
                     EVEHDPEEIWQSVLTAARAVLKEVGAQSIEAIGITNQRETTLVWDRATAKPLANAIVW
                     QDRRTAMQCDQLKREGWDAHVAETTGLVIDPYFSATKLAWLLANVSGLAERARAGEIC
                     FGTVDSFLLFRLTGGKTHAIEASNAARTMLYDIRSGRWNERLLDRLGIPRALLPELRD
                     SQDDFGATESDHFGVAIPIRGVAGDQQAAAYGQACFAPGMLKATYGTGCFLLANTGEA
                     KVASTARMLATVFYQLEGRRTFALEGSIFMAGATVQWLRDSLGLIAEAAESEALAHRA
                     DPKSRVYLVPAFQGLGAPHWDAEARAAILGLTRAASAADIVAAGLEAVAFQTRDLLLA
                     MRADMAQSGIPASESLRVDGGMTANNWFLQRLADILGERVEVARHPETTALGAAFLAG
                     QASGFYGSQEELQRARVSSRAFDPSMGVDEREARYAGWLEALTKVRSKA"
     gene            5622..5990
                     /locus_tag="VN518_04420"
     CDS             5622..5990
                     /locus_tag="VN518_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020088020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HXE03851.1"
                     /translation="MAIASIDFQMLANAGFPPVRYAAGNVIFAEGDRGDAMYVIRNGE
                     VTVEHGGRVIETLGGGGIFGEMALIDGSVRSATVRAKTDCEVAPINEKTFLFLVHETP
                     FFAIAVMRALAGRLRTMNQP"
     gene            6096..>6414
                     /locus_tag="VN518_04425"
     CDS             6096..>6414
                     /locus_tag="VN518_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03852.1"
                     /translation="MESLANQALRRSRNMDLPTLGRDALAGVISAIVQIAYCISFSAL
                     IFQGAIASGFSLGLAALIMGTVVTGLIVALTSTLSPADAGPDTPAVAVMSVLAGSIAA
                     ALAA"
BASE COUNT         1031 a         2045 c         2129 g         1209 t
ORIGIN      
        1 ggggcgccgg acatgctcgg tgttccgggc atggcgtcgg gtgaacccgg cgatgccacg
       61 gtcgtgccgg ctggaggtgc gggaggcggt ggcggcggtt cggtgatcgc cttcagggcc
      121 tcgggcgagg ccgggcccga ctccatcacg acctcgatct gtttggcctc tccggccgcc
      181 acgctgaact tgctcgtgta gctttcaccg ttgtggttgg ccacaacggc atagtcgccg
      241 agggcgagaa tatgggtcgg cagcgcgccg gtattttcct tcaccacgtc gccggtcgtg
      301 gtgaggatgc tccacttggt gtcggccaaa gcttcaccac ctgcactctg caccaacttg
      361 aaggtgaccg gtgctgcggc gtgcttgacc gtcgcttcgg tgatcttgcc gggctcgacg
      421 gtcacgtcgg cgcgcaccgt tccattagca tcgccatagg tgctgaccag gtgataggcg
      481 ccggcattca gcctgatgac gacgccgggc ttggcgtcgt ccaggatctt gtgccgattg
      541 ccgaactgat cctcttcatc tgagaggatg tcgaaatgaa cgctcgaggc cggcaacgct
      601 gcgccgccgg cgagaacggc agcaagcttc aatcctccgg tgttgaggac aaacgtctcc
      661 tcgagagacc ggccgctctc gaccttgatc ttcttggtga ggttggaaag gccgtaggcg
      721 gcgttgacca ggtattctcc cggaaccaag gccgctgtcg gcatggcttc atgatgggtg
      781 ctgacgagct tgcgggcgcc gttcgctcca ggctgcggcg tgaacacgcg ccagatcagc
      841 ccggttttga cctgaggtcc accttcggta agcagggctt tgaaggtcac ggggaccggt
      901 gccgattgct ccgcgccctg ggtgcccggt gcagcgggcg tccgcgccgg ggcagcactt
      961 tccttcggcg gaattgccgc gagcttcgcc atgtcggtct gggccggagc ctgcggtccg
     1021 atcaccgcgg atgattgagg agcttgcggc cccttgggtt gcgcactttt cgcctgggcg
     1081 gtttccgctg gcgccgttgg ggcggagggg tttgccgatg cggacttttg cgcggccggc
     1141 gcggtcggcg cggcttgtgc cggcgggttg ggctgagccg cgctcggcgc ggcgttggca
     1201 tgcggcgtgg cgctcgcggg gcccggagtt gccgtatccg cgcgaggagg cgggggcgct
     1261 gctgccggcg gggccggtgg agcggcaggg ctcttgctgg ccacgggcac gggtcgcggc
     1321 gccgatttgc ttgcggcatc gagcacggcg ttaaggtctt gcttcaggtc gttggcgttg
     1381 gcagcggtca ggaactggcc cccggtggtc ttcgccacgc aactaagagc cttgacggtt
     1441 tgcttcgcct tggggtcgaa gccgatgaca tgaatgcgca agcccggcgc cgcttgcttc
     1501 agcgatttta ccgtggcgca gacattggcc ttgcaggtgt cgggcccgtc ggtgataagc
     1561 acgacatcga gacccgtggc gggcgtttgc ttcgcagcat cgaccagcgc ggccgcgact
     1621 ggcttcgtgg cctttggctt gaagcccgca ccgaacaaga gcttgccggg cgatttcgcg
     1681 ctgagctcgc cgggcttcgc caaaagctca gcctcggtgc atgctccgcc accgcggtgg
     1741 ccgaatacta cgagaccgaa cgcgatgcga tcttcatagt ccgtgaaggc ctgagtaagc
     1801 gtcgagcgta cgatgccgac cttgctctgt ccgccgagcc tcgcccacat gctgatagag
     1861 ccgtcgagca agatgattgt cggcgcaagt ccttgctgcg cccggggagc ttgctgatcc
     1921 tcggcgcttg cgtggagcgc aaataggagg aggatgggcg cgagcaaaag cccaagccct
     1981 gcgcaacgct tccctcccat ggcgtttcgc tcccgtcagg cccccggcct cggccggacc
     2041 ttataccccg ttcgaccacc cccacaacgt aagcgaaatt cggccatgac taaggccgat
     2101 ttggggtcgc gcgcgctgtc ctgatttcca atgagtcgtt gtttcgcaaa gcttttcgcg
     2161 ataggggaac gcgccccccg ccatgccaaa ggccatgacc aatcccatca tcgctcattt
     2221 gctcacccgc cgctcggtct ctgccaacag cctcggagag cctgggccga gccaggacga
     2281 gatcgagcag attctgactg ctgccgcgcg ggtgcccgac cataagaagc tcgtgccatg
     2341 gcgctttctg ctgtttcagg gtgaggcgcg ggccgcgttc ggcgagatcc ttgccgagat
     2401 ctgcaagcgc gaggagaagg acccgagcca aatccggctg gagaccgagg ccaaccggtt
     2461 catgcgcgct ccgctcgtga ttgccgcgat ctcgcgcgtg gtgaacaatc cggcggcgcc
     2521 ggaatgggag caggtgcttt cggccggggc cgcgtgtcag aacctgctca tcgccgccac
     2581 cgcgcttggt tacggtgccc agtggatcac cgagtggtac ggctacagcc aaggcgtgcg
     2641 cgccgcgctc atgcttgccg acaatgagcg ggtggccggc ttcgtctata tcggcaccgc
     2701 caaggagaag cccgaggagc gggagcggcc gtcgctcgcc gacattgcgc aagcctggcg
     2761 gcggtgagcc cagacctcag tagtcgaatt gctcccgcag gatgcgctca gcgaggctgt
     2821 ggccgggatc gaacatcatg aagatgtcga cgtcgctcgc ctccctgatc tccacggcga
     2881 gcacgtcgcg aaactcggtg ttgtcggcca ccgcgctgac cggtcgctta tcaggctcga
     2941 gcacctcgat tagaacgacg gcctcgtttg gcaggatcgc gccgcgccag cgccgcggcc
     3001 ggaacgggct gatgggggtc agcaccatca gcggcgcgtt gatcggcaag atcggcccat
     3061 gcgcagatag gttgtaggcg gtgctcccgg ccggggtggc caggagcacg ccgtcgcaga
     3121 tgagctcatc gagccgcacc cggccgtcga ccgagatgcg gagctttgcc gcctgataga
     3181 tctgccggaa cagcgacacc tcattgatgg cgagcgcctt gtgctcggct ttgtcgcggt
     3241 tgtaggccgt catgcgcaga gggtggatga cgttgacctc cgccttgtcg aggcgttcaa
     3301 gcagctcgtc ttcccggtag tcgttgagca ggaagccgac cgagccgcgg ttcatcccgt
     3361 agatcgggat gcggttgtta atatagcggt gcatggtctg cagcatctgc ccgtcgccgc
     3421 ccaacgcgac gatcacgtcg gcctcttctg gcgccgtatc gccgtagcgg gcgacgaggt
     3481 cctgccgcgc tccttgcgct tctttcatct cactcgcgac aaaggcgatg ttgcgaaact
     3541 tggacatggt cgcttcgatg gacttggagc gggcctagag agcgggcact ataaaggatg
     3601 tggcgcccgt tcattgcaag cctatcggtc gcatcgggga gaatggtgaa tcgatgacca
     3661 aggacgatcg tccggttctg gtttacacga cttttgcaac cctcgacgac gccaagcggg
     3721 tcggagacgc tctcgttgcc gcgcggcttg cggcttgcgt caacatcttc cccggcatga
     3781 tctcgatcta tgagtggaag ggcgcgcgcg agatcgccga cgaggtcgcc atgatcatca
     3841 agacgcgagc ttcgcttgcc gacgccgtgc tggccgagac caagcgcctc cacccctacg
     3901 agctgccggc acttctcgtg ctgccgacgg aaggggggag cgaggaatat tgcggctgga
     3961 tcctgagcca gaccggacgc gctccatgag cgagcgccgc ttagtgctcg ccatcgacca
     4021 gggcacgacc agcacccgcg ccatcgcctt cgatcaagag gcgcgcgttc gcgccgcagc
     4081 ccaggtgcct ctcacccaga tctatcccgc gcctggagag gtcgagcacg atccggagga
     4141 gatctggcaa tcggtgctca cggcggcgcg cgccgtgctc aaggaggtcg gcgcgcaatc
     4201 gatcgaagcg atcggcatca ccaatcagcg cgaaacgacg ctggtgtggg accgcgctac
     4261 ggccaagccg ctcgccaacg ccatcgtctg gcaggaccgg cgcaccgcca tgcagtgcga
     4321 tcaactcaag agggagggct gggacgcgca tgtcgcggaa accacgggcc tcgtcatcga
     4381 tccttatttc tcggcgacga agcttgcctg gctgcttgcc aacgtctccg gccttgccga
     4441 gcgggcgcga gcgggagaga tctgcttcgg cacggtcgac agctttctcc tgttcaggct
     4501 gaccggcggc aagacgcacg cgatcgaagc gagcaacgcc gcccgcacta tgctctacga
     4561 catcaggtcc gggcgctgga acgagcggct gctcgaccgc ctcggcattc ctcgcgcgct
     4621 ccttcccgag ctccgcgaca gccaggacga tttcggcgcg acggagagcg accatttcgg
     4681 cgtggccatc ccgatcaggg gcgttgccgg cgaccagcag gccgcggcct acggtcaggc
     4741 gtgttttgcg ccaggcatgt tgaaggcgac gtatggcacc ggttgctttc tgctcgccaa
     4801 tacgggtgag gccaaggtgg cttcgaccgc gcgcatgctt gcgaccgtct tttatcagct
     4861 cgaaggtcgc cgcacgttcg ccttggaggg atcgatcttc atggccgggg ccacggtgca
     4921 atggctgcgc gacagtctcg gcttgatcgc cgaagctgcg gaaagcgaag ctctcgctca
     4981 ccgggccgat cccaaatctc gcgtctatct cgtcccggcc tttcaggggc tcggcgcgcc
     5041 gcattgggac gcagaggcac gcgccgcgat ccttggcctg acacgagccg cctctgccgc
     5101 cgacatcgtg gcggcgggcc tcgaagccgt cgccttccag acccgcgacc tgctcctcgc
     5161 catgcgcgcg gacatggcgc aaagcggcat cccggcttcc gagagcttgc gcgtcgatgg
     5221 cggcatgacc gccaacaact ggtttctgca acgcctcgcc gacatcttgg gcgaacgggt
     5281 cgaggtcgcg cgccatccgg aaacgacggc gctcggcgct gccttcctcg ccggccaagc
     5341 gagcggcttc tatggcagcc aggaagagct gcaacgggcc cgggtgtcgt cgcgcgcctt
     5401 tgatccctcc atgggcgtcg acgagaggga ggcgcgctat gccggctggc tcgaggcgct
     5461 aacgaaggtc aggtcgaagg cctgagcccc ttccgttaag gctggaccca agaggctggc
     5521 atcattgcct gttagctttt cgcggccttt atcgaatcgt cacatgatcg tcatgagtgt
     5581 atcctcaaag gctcgtacca gggagcatgg ggacggcaca gatggcaatt gcatcaatcg
     5641 acttccaaat gttggccaat gcgggatttc caccggtgcg ctatgccgcc ggcaatgtga
     5701 tctttgccga aggcgataga ggcgacgcca tgtacgtcat ccgcaacggc gaagtcaccg
     5761 tcgaacacgg cggccgcgtc atagagaccc tgggcggcgg cggcatcttc ggcgagatgg
     5821 ccttgatcga tggatcggtg cgaagcgcca cggtccgcgc caagaccgac tgcgaggtgg
     5881 ccccgatcaa cgagaagacg ttccttttcc tcgtccacga gacgccgttc ttcgcgattg
     5941 ccgtgatgcg cgcgcttgcc gggcggctgc ggaccatgaa ccaaccctag ccgctgcttg
     6001 cggtcgcgca cgcgctattt ttggcctttt atttgctgcg ccccccggcc atgatcggcc
     6061 ggagcctaaa cccgtgaaca ttctgaacgc tgtccttgga atcgctcgct aatcaggcgc
     6121 tgcggcgctc gcgcaacatg gatttgccga cccttgggcg cgacgctttg gcgggcgtca
     6181 tctcggcgat cgtccagatt gcgtattgca tctccttcag cgcgctgatc ttccagggcg
     6241 cgatcgcctc cggtttctcg cttggccttg ccgcgctgat catgggtacc gtggtgacgg
     6301 gcctcatcgt ggccctaacc tcgacgctgt cgccggcgga cgccgggccc gatacgccgg
     6361 ctgtcgccgt catgagcgtg ctcgccggct ccatcgccgc tgcgctcgcg gcca
//
LOCUS       DATNAE010000195         4766 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2292687, whole genome shotgun sequence.
ACCESSION   DATNAE010000195 DATNAE010000000
VERSION     DATNAE010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4766
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2292687"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..991
                     /locus_tag="VN518_04430"
     CDS             <1..991
                     /locus_tag="VN518_04430"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HXE03853.1"
                     /translation="AFANILGSGLCFLFSGQLAKLCTLRYTLIMPIVLSLVYIGAFEG
                     TRQWGDLYSLLFFGILGWAMKHFKWPRPPLVLGFILGKVLERYMFISIQRYGAEWMER
                     PIVIVFFCMAALSLLRPFIQDVRRHGGAKGMLSDFGAPKLKGRMVFPFFMLCLFAVMV
                     IDGTHWNIEAKIIPLIVGSGAILFVSISLLNEIFARPGFAGVGEVGEKAKAMAEQQAA
                     VAGKHDKIHMDIASTIEHLPTSVKLTRGSIFFGWLVGFLCSMAVIGLIPTVPLFVAGY
                     MRLEGRERWTIVIPYMLCITIFIYFLFDQLLAIPWPPTYLGDFFPALKAIPSV"
     gene            complement(969..2546)
                     /locus_tag="VN518_04435"
     CDS             complement(969..2546)
                     /locus_tag="VN518_04435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HXE03854.1"
                     /translation="MPNDSEAVTYQRFNKREIRSVMIGLLLAMFLGSLDQTIVATTLS
                     TMARDLEGWEIMSWVVSGYMITSTTVTPIYGRLSDQFGRRPLLLVAIFLFLASSIFCA
                     LADTMPMLVLGRFLQGIGGGGLRSVAMAVVADVIPPRERGRYQGYFSINFAIATVLGP
                     VLGGLFARYLTWHWIFWINVPLGLVALALSNRQLKRLGRPARRPRVDWFGALLILLGS
                     TPLLYALGEVEQAGGWGHARVLVPLGLGLASVLLLVWWETIAREPMLPLRLFRNRTFT
                     AALTSTSLANMIFTSLVVLIPLNYQLLGLPADQAGIRLIPFTISGAIASFSVGYCVSR
                     LGRARIFPLVGCALLTTMCGVMAWTGIGRSLASDLTVTFLLGATLGFQFNPVNVLVQN
                     SLRMEDNGIGVSSQMFFRLMAAAFGVALFTAFLIGTLSAGARTVPGHEALGPEPGFAL
                     LHLDLRRADLDPALITSLAQTIHDAFSQIFLIMTGLAFLAFLATLRLKDEPLRGYAPS
                     PAKPEERAVAATRSESP"
     gene            2759..3430
                     /locus_tag="VN518_04440"
     CDS             2759..3430
                     /locus_tag="VN518_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HXE03855.1"
                     /translation="MALTFKPILDILPKPQRTLWDELAAIPGEFVLYGGTAIALQLAH
                     RQSIDFDFFADQPIDPFRLEAAVPFMAGARIINRDKNTLTGIIDRGGPVAISFFGLPA
                     LRRLAPALISPDNGIKIASLLDLAGMKVSVVQARAEAKDYLDLDALMTRAGIDLPTAL
                     AAGREIYGKSFNPQITLKALSYYHDGNLDQLPEETKRRLAFAARDVDLDSLPAVGDSS
                     DQSAH"
     gene            3440..3730
                     /locus_tag="VN518_04445"
     CDS             3440..3730
                     /locus_tag="VN518_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03856.1"
                     /translation="MKPIPFIPEIEEVARRIIWFEPPKQALEDPVRFMAYAMARATHE
                     DMQAIRRHVSDDDFREALDKAPPGIIDPRSWAYWNLKLGRFPPPPVPRRKFG"
     gene            complement(3798..4526)
                     /locus_tag="VN518_04450"
     CDS             complement(3798..4526)
                     /locus_tag="VN518_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HXE03857.1"
                     /translation="MARIRVGIGGWVFAPWRGSFYPEGLKQAKELDYASSRLSSIEIN
                     GTFYGAQKLSSFEKWHRETPADFVFSVKGTRYATYRTELGEAGPSVERFFSTGVLALK
                     EKLGPILWQLPGNARFDAGRLEAFLQLLPKELEGLKIRHVFEAPHDSFASQEALDLLR
                     KYGVAVPMVDSEGRVLVAERTADFLYVRLRRAVEDEPAGYSPEALQEWAEKLKAAGVD
                     CFVYFINGAKIRCPAAAQALIKLL"
     gene            complement(4526..>4766)
                     /locus_tag="VN518_04455"
     CDS             complement(4526..>4766)
                     /locus_tag="VN518_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03858.1"
                     /translation="RDLLTMDFSGWPIVVHREAEDDLVTSFCAALDARKANIPWQGEG
                     PLPVERLCRDAPDTPQDVPIHPAAEKFWRDKGYLS"
BASE COUNT          894 a         1511 c         1529 g          832 t
ORIGIN      
        1 cgccttcgcc aacattctgg gctcgggcct gtgcttcctg ttctcgggcc agctcgccaa
       61 gctctgcacg ctccgctaca cgctgatcat gccgatcgtg ctgagcctgg tctatatcgg
      121 cgccttcgag ggcacgcggc aatggggcga cctctattcg ctcctgttct tcggcatcct
      181 cggctgggcg atgaagcact tcaaatggcc gcgtccgcct ttggttctgg gcttcattct
      241 gggcaaggtg ctcgagcgct acatgttcat ctcgatccag cgctacggcg ccgaatggat
      301 ggagcgcccg atcgtcatcg tgttcttctg catggcggcg ctgagcctgc tcaggccttt
      361 catccaggac gtgcggcggc atggcggggc caagggcatg ctcagcgatt tcggcgcgcc
      421 gaagctgaag gggcggatgg tgttcccctt cttcatgcta tgcctgttcg cggtcatggt
      481 gatcgacggc acccactgga acatcgaggc gaagatcatc ccgctgatcg tcggctcggg
      541 cgcgatcctg ttcgtcagca tcagcctatt gaacgagatc ttcgccaggc ccggcttcgc
      601 cggcgtcggc gaggtcggcg agaaggccaa ggcgatggcc gagcagcagg cggcggtcgc
      661 cggcaagcat gacaagatcc atatggacat cgcctccacc atcgagcatc tgccgacgag
      721 cgtgaaactc acgcgcggca gcatcttctt cggctggctg gtgggcttcc tctgctcgat
      781 ggcggtgatc ggcctcatcc caaccgtgcc gctgttcgtc gccggctata tgcggctcga
      841 gggacgggag cgctggacga tcgtgattcc ctacatgctc tgcatcacga tcttcatcta
      901 cttcctgttc gaccagctgc tggcgatccc ctggccgccg acctatctcg gcgacttctt
      961 tccggcgctc aaggcgattc cgagcgtgta gccgcgacgg ccctctcctc cggtttcgca
     1021 ggagagggcg cgtagccgcg cagcggctcg tccttgagcc gcaaggtcgc aaggaaggcg
     1081 aggaaggcga gccccgtcat gatcaggaag atctgcgaga aggcgtcgtg gatcgtctgg
     1141 gcgagggacg tgatcagtgc cggatcgaga tcggcgcgcc tgagatcgag atgcagcagc
     1201 gcaaatccgg gctcgggccc aagcgcctcg tggcccggca cggtgcgggc gccggccgac
     1261 aaggtcccta tcaggaaggc ggtgaagagc gccacgccga aggccgccgc catcagccgg
     1321 aagaacatct ggctcgacac gccgatgcca ttgtcctcca tgcgcagcga attctgcacc
     1381 agcacattga ccggattgaa ctggaagccg agcgtggcgc cgagcaggaa ggtgacggtg
     1441 aggtcggagg cgagcgaacg gccgatgccg gtccaggcca tcaccccgca catggtggtg
     1501 aggagcgcgc agccgaccag cgggaagatg cgggcccgtc ccaaacgcga cacgcaatag
     1561 ccgaccgaga agctcgcgat ggcgccgctg atggtgaagg ggatgagccg gatgccggcc
     1621 tggtcggccg gcaggcccag gagctgatag ttgagcggga tcagcaccac gaggctggta
     1681 aagatcatat tggcgagcga ggtcgaggtg agcgccgcag tgaaggtccg gttgcggaac
     1741 aggcgcagcg gcagcatcgg ctcgcgtgcg atcgtttccc accacaccag cagcagcacc
     1801 gaggcgagcc cgagccccag cggcaccagc acgcgcgcat gtccccagcc gccggcctgc
     1861 tcgacctcgc ccagggcata gagcagcggc gtcgagccga gcaggatcag cagtgcgccg
     1921 aaccaatcga cgcgcggccg ccgcgccggc cggccgagcc gtttcagctg gcggttggac
     1981 agggcgagcg ccaccaggcc gagcggcaca ttgatccaga agatccagtg ccaggtgagg
     2041 tagcgggcga acaagccgcc gagcaccggt ccgaggacgg tcgcgatcgc gaaattgatc
     2101 gagaaatagc cctgatagcg gccgcgctcg cgcggcggaa tcacgtcggc gaccaccgcc
     2161 atcgccaccg agcgcaagcc gccgccgccg atgccttgca ggaagcgccc gagcacgagc
     2221 atcggcatgg tgtcggccag ggcgcagaag atcgagctcg ccaggaacag gaagatcgcg
     2281 accagcagca gcggccgccg gccgaactga tcgctcagcc ttccgtagat cggcgtcacg
     2341 gtcgtcgagg tgatcatata gccggagacg acccaggaca tgatctccca gccctcgaga
     2401 tcgcgcgcca tggtcgagag cgtggtcgcg acgatcgtct ggtcgaggct gccgagaaac
     2461 atggcgagca gcagcccgat catgaccgag cggatctcgc gcttgttgaa acgctggtag
     2521 gtgacggctt cgctgtcgtt gggcattggc gagactataa aggccttgtt cgggggcgcc
     2581 cactaaccag gcagccataa cttccgaaac cgggcacggg agagcgaaag ccactctccc
     2641 ggcaaccgcc gatgccgact tcgagcgccc cgcaaccggt tatggtattc ttgcacccag
     2701 gcgcggctcc agcctgtacc tggcaggctg ccggccgaag cttatcggaa gggcgtgcgt
     2761 ggctttgacc ttcaagccga tactcgacat cttgccgaag ccgcaaagga ccctgtggga
     2821 cgaactcgcc gcgattcctg gtgaattcgt gctctatggc gggacggcga tcgcgctcca
     2881 gctcgcccac cggcagtcca tagatttcga tttttttgca gatcagccga tcgacccctt
     2941 caggcttgaa gcggccgtcc cgttcatggc tggcgcgcgg atcataaatc gcgacaaaaa
     3001 taccctgact ggaattatcg atcgtggcgg cccggtcgcc atttcattct tcggcttgcc
     3061 tgctttgcgc cggcttgcgc ccgctctcat ctctcctgac aacgggatca agatcgcatc
     3121 tctcctcgat ctcgccggta tgaaagtttc ggtcgtgcag gcgcgtgcgg aagcgaagga
     3181 ctatctcgat ctcgatgcgc ttatgacacg ggccgggatt gatttgccga ccgctctcgc
     3241 agcgggccga gagatctatg gcaaatcctt taatccgcag ataactctga aggcgctgag
     3301 ttactaccac gatggtaacc tagatcagct tcctgaagaa acaaaacgtc ggctggcttt
     3361 tgccgcgcgc gacgtggatc tcgactccct gcccgctgtc ggcgatagct ccgaccagtc
     3421 agctcattaa ggcagagcca tgaaacctat cccgttcatt ccggaaatcg aagaagttgc
     3481 gcgccggatc atttggttcg agccaccaaa gcaggcgctc gaggaccctg tccgtttcat
     3541 ggcctacgcg atggcaaggg ccacgcatga agatatgcag gcgatccgcc ggcatgtgtc
     3601 cgatgacgac tttcgtgagg ctctggacaa agcgccgccc ggcatcattg atccgcgctc
     3661 ttgggcctat tggaatttga agctcggccg attcccgccc cctcctgtcc cacggagaaa
     3721 gttcggataa ggcggtcgca ggcaaagcct ccttggtcgc caacagcagc tgcgctgcag
     3781 ctatgacggc aaagagccta tagcagcttt atcagcgcct gcgccgccgc cgggcagcgg
     3841 atcttggcgc cgttgatgaa atagacgaag caatcgacac ccgccgcctt caatttctcc
     3901 gcccattcct gcaaggcttc gggcgaatag ccggcgggct cgtcctcgac ggcgcggcgc
     3961 aggcggacat agaggaagtc ggcggtgcgc tccgcgacga gaacgcggcc ttcggaatcg
     4021 accatgggca cggcgacgcc gtatttgcgc aggagatcga gcgcctcttg cgaggcgaag
     4081 ctgtcatgcg gcgcctcgaa gacatggcgg atcttcagcc cttcgagctc cttgggcagg
     4141 agctggagga aggcttcgag gcgcccggca tcgaagcgcg cattgccggg cagctgccac
     4201 aggatcggcc cgagcttctc tttcagcgcc agcacgccgg tcgagaagaa gcgctcgacc
     4261 gaggggccgg cctcgccgag ctcggtgcgg taggtcgcgt aacgcgtgcc cttgaccgag
     4321 aagacgaaat cggccggcgt ctcccggtgc catttctcga agctcgaaag cttctgcgcg
     4381 ccgtagaagg tgccgttgat ctcgatcgag ctgagcctgg agctcgcata gtcgagctcc
     4441 ttcgcctgct tcagcccctc gggatagaag ctgccgcgcc aaggcgcgaa aacccagccg
     4501 ccgatgccga cccgaatgcg cgccatcagg aaagatagcc tttgtcgcgc cagaatttct
     4561 cggccgcggg atggatcggc acgtcctgcg gcgtgtcggg cgcgtcgcgg cacagccgct
     4621 cgaccggcaa cggcccctcc ccctgccagg ggatgttggc cttgcgcgca tcgagtgccg
     4681 cgcaaaagct ggttacgaga tcatcctcgg cctcgcgatg aacgacgatc ggccagcccg
     4741 agaaatccat ggtcaggagg tcgcgg
//
LOCUS       DATNAE010000196         6420 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_188822, whole genome shotgun sequence.
ACCESSION   DATNAE010000196 DATNAE010000000
VERSION     DATNAE010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6420
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188822"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            177..926
                     /locus_tag="VN518_04460"
     CDS             177..926
                     /locus_tag="VN518_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013169197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HXE03859.1"
                     /translation="MGIYKKMRLDRILVDRGLAVTRSRAADLIRLGAVSVDGRPALKP
                     GALIEPGARLMVDRHASPFVSRGGLKLAAALDAFGLDPKGLIALDIGASTGGFTEVLL
                     ARGAERVFAVDVGREQLHWKLREDQRVVVREGTDARVLDAAVVSGPVGAIVADVSFIS
                     LTKALPAALGLAAPGAWLIALVKPQFEAGREAVCKGGIVRDAQARARAVAEVRAFINA
                     TPGWKVFAEMPSPIAGGSGNEEVLIGASHGA"
     gene            1973..2143
                     /locus_tag="VN518_04465"
     CDS             1973..2143
                     /locus_tag="VN518_04465"
                     /inference="COORDINATES: protein motif:HMM:NF017743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03860.1"
                     /translation="MAASKVPKVVMVSCNPGTCARDLRILVDGGYRITRVVPVDQFLF
                     SPHIELVAELEK"
     gene            complement(2201..2905)
                     /locus_tag="VN518_04470"
     CDS             complement(2201..2905)
                     /locus_tag="VN518_04470"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009210623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HXE03861.1"
                     /translation="MERYNNIVILTGAGLSAESGLGTFRGPGGLWENFRIEDVATPEG
                     FARDPVLVHNFYNLRRSWHKKAWPNAAHLALARLEREHGNVLTVTQNIDALHEAAGTK
                     NLIHMHGELERALCAACGVSAPWTEDLSIETLCARCGEAAMRPDVVWFGEMPREMERI
                     YEALGACDLFISIGTSGTVYPAAGFVMEARAGGAHAVELNLEPSDGADLFLERIEGPA
                     TEIVPAYVDRLLRAKP"
     gene            complement(2918..4012)
                     /gene="aroC"
                     /locus_tag="VN518_04475"
     CDS             complement(2918..4012)
                     /gene="aroC"
                     /locus_tag="VN518_04475"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009210628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HXE03862.1"
                     /translation="MSHNSFGHLFRITTWGESHGPAIGCVVDGCPPGIQLEVADIQTY
                     LDKRKPGQSRYTTQRQEPDQVRILSGVFTDEASGEQVTTGTPIALEIENMDARSKDYS
                     EIKDKFRPGHADYTYLMKYGVRDWRGSGRASARETASRVAAGAVARKVVPGMRVRGAL
                     VQVGPHAIDRRTWDWSEVERNPFFSPDAKAVKKWGDFLDKVRKEGSSVGAIIEIVAEG
                     VTAGLGAPVYGKLDQDIASGLMSINAVKGVEIGAGFAAASLSGEENADEMRIGRDGTP
                     EFLTNNAGGILGGISTGQPIVARFAVKPTSSILTPRRTIDAAGNETEISTKGRHDPCV
                     GIRAVPIGEAMMAIVLADHYLRHRGQTGKG"
     gene            4182..4967
                     /locus_tag="VN518_04480"
     CDS             4182..4967
                     /locus_tag="VN518_04480"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HXE03863.1"
                     /translation="MLPTTLRDGAYVPKRVKEEIDFDGPIERGAKKMRAQRVQEFLTL
                     NGFAVSIDGDFGPGTEKRVRDFQAARGLPATGVVDMKTHEEPVAPIVKALNPIAAGGK
                     SLSTLAAAYAKQHIKNHPKEAGDDNRGPWVRSYLDWDGKDARWCAGFVCFALQQAAHT
                     LGVNLPIDSSSSCDVLAMEAKTAGKFVPESKVKSGAFAKKDLVPGTFFLVRKVPGDWV
                     HVGIVTEPAKESFDTAEGNTGSSNGFEAAERARDYRSKDFIVW"
     gene            5139..5459
                     /locus_tag="VN518_04485"
     CDS             5139..5459
                     /locus_tag="VN518_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03864.1"
                     /translation="MKLMVLLASLGLASALLFVSADGADAVSSRHQAAHARAHAQHYA
                     RTNRHSPRRYAARRGGYHGKMTLNEILYGPAGMPPAPHDFGPHFDYPPEPLNGGITSS
                     PYLH"
     gene            complement(5513..6331)
                     /gene="fabI"
                     /locus_tag="VN518_04490"
     CDS             complement(5513..6331)
                     /gene="fabI"
                     /locus_tag="VN518_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013501146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HXE03865.1"
                     /translation="MAEAAKLMAGKRGIVFGVANNRSIAWGITKACYDQGAEIALTYQ
                     GDAIKKRVEPLAAEIGSKLVLPCDVTDEGSVEAVFKALGDSWGSIDFLVHAVAFSDKE
                     ELDGRYVDTSKDNFTQTLLVSCYSFTALAKRAERLMPNGGSLLTLSYYGAEKVMPHYN
                     VMGVAKAALEASVRYLAADLGKENIRVNAISAGPIKTLAASGIADFRYILKWNEYNSP
                     LRRTVSIEDVGGGALYLLSDLGRGVTGEILHIDAGYHVVGMKNQDAPDISVAKE"
BASE COUNT         1076 a         2080 c         2103 g         1161 t
ORIGIN      
        1 tcggacttcg cacttttcgg tgaggtaagc cgtcgtactt tggctcacga gacctccgcg
       61 atggcgcgcc gagcggtgcg cttcgcagta tatagttggg cggtgccgtc cgctcccggt
      121 tcgtcatccg cacgcaagga aacccccacg cggggcgaag catatgagaa gcgaatatgg
      181 gcatctacaa aaaaatgcgc ctcgatcgaa ttttagtgga cagaggtctg gccgttaccc
      241 gctcccgcgc cgccgacctg atccgcctcg gcgcggtgag cgtggacggc cggccggcgc
      301 tgaagcctgg ggcgctgatc gagcccggcg cgcgcctcat ggtcgaccgg cacgcctctc
      361 ccttcgtctc gcgcgggggc ctcaagctcg ccgccgctct cgatgccttc ggcctcgacc
      421 ccaagggcct catcgcgctc gatatcggcg cctcgaccgg aggcttcacc gaggtattgc
      481 tggcccgcgg ggcggagcgt gtgttcgccg tcgatgtcgg ccgcgagcag ctgcactgga
      541 agcttcgcga ggaccagcgt gtcgtggtcc gtgaggggac cgacgcaagg gtcctcgatg
      601 ccgcggtcgt cagcggccct gtcggcgcca tcgtcgccga tgtgagtttc atcagcctga
      661 ccaaggcgtt gccggcggcg ctcggcctgg cagcgccggg cgcctggctc atcgccttgg
      721 tgaagccgca attcgaggcg ggccgcgagg cggtgtgcaa aggcggcatc gtgcgcgacg
      781 ctcaagccag ggcaagagcc gtggcggagg tgcgggcttt catcaatgcc acaccaggct
      841 ggaaggtgtt tgccgaaatg ccctcgccga ttgctggcgg cagcggcaac gaggaagttt
      901 tgatcggcgc cagccatgga gcttgaggca ggatcgagcg tctcggcgcg caaggcggcg
      961 gcctggcgca aggccccgac gggccgctct tcgtgccctt cacgcttcca ggcgagttgg
     1021 tgaaggtggc ggccgagcca ggagagcccc gcgccgaggc gcttgccatc ctcgagccga
     1081 gccccgagcg cgtcgcgccg gtctgccaat atttcggcac ctgcggcggc tgtgccctcc
     1141 agcatataga ggcgagagcc tatctcgcct ggaagcgcga gcaggtgatt gcggcgcttg
     1201 cctcgcgcgg gctcgaggtg ccggtcgggg aggtccgcgc ggtgcctctc gccagccggc
     1261 ggcgcgccgc cttcacgctt ggccgcacgg ctcgcggcgt tgctttcggt taccgcgccg
     1321 cccgctcgca cgccattgtc gatatcgccg cctgcccggt gctcaactca agcatcgtcg
     1381 ggcgcctgcc caagctcaaa tccctgctcg cgttgctgct tggcggcaag cgcgaagcgc
     1441 gcgtgactgt gacggaggct gatcagggcc tcgatgtcgc ggtcgagggc atccgcccct
     1501 cggcggggtt attcgccaag ttggccgcca cgggtgcaag aatcggagcc ccccgcatca
     1561 tgatcgacgg cgagagcgtc attctcgccg gcgagccttc ggtgacgctt tccggcgcgc
     1621 aagtgcggct gccgccagga gccttcctgc aagcttcgcg cgagtcagac gcggtgctgg
     1681 cggggctggt caaatcgggc gtggggcgcg ccaagcgcgt ggcagatctg tttgccggca
     1741 tcggcacctt caccttcgcg ctcgcccgaa gcgccgaggt cgacgccttc gagcaggatg
     1801 aagcggcaat cgctgctcta acgggggcag cgcggacaac gccgaagctg aagccggtgc
     1861 gcacattttc ccgcgatctc ttccgcgccc tctctccgca cgcgagcttg cccgctacga
     1921 cgctgtcgtc ctcgatccgc cgcgggcagg cgccagggcg caggccgagg cgctggccgc
     1981 ttcgaaggtg ccgaaggtcg tcatggtctc gtgcaatcca ggcacctgcg cccgcgacct
     2041 gcgcatcctc gtcgatggcg gctaccgcat cacgcgcgtg gtccccgtcg accagttcct
     2101 gttctcgccg catatcgagt tggtggctga gctcgaaaag tagggtgggc aaagcgaagc
     2161 gtgcccacct ctctcggtgg gcaacgtgcc gttgcccacc ctacggctta gcgcgaagca
     2221 aacggtcgac ataagccgga acgatctccg tcgctggccc ttcgatcctc tcaaggaata
     2281 gatcggcgcc gtctgatggc tcgagattga gctcgacggc atgggcacct ccggcacgcg
     2341 cttccatcac gaagcctgca gcgggataga cggtgccgct ggtgccgatg gagatgaaca
     2401 aatcgcaggc gccgagcgcc tcatagatgc gctccatctc gcgcggcatc tcgccgaacc
     2461 acaccacgtc ggggcgcatg gcggcttcgc cgcacctcgc gcagagggtc tcgatggaca
     2521 gatcctccgt ccacggcgcg cttacaccgc aggcggcgca cagcgcacgc tccaactcgc
     2581 catgcatgtg aatgaggttc tttgtgccgg cagcctcgtg cagcgcatcg atgttctggg
     2641 tgacggtcag cacattgccg tgctcgcgct cgagcctggc aagggcgaga tgggctgcgt
     2701 tgggccacgc ctttttgtgc caggagcggc ggagattgta gaaattgtgg acgaggaccg
     2761 gatcgcgggc gaagccttcg ggcgtcgcca cgtcctcgat cctgaaattc tcccacagcc
     2821 cgccaggccc gcgaaaggtg ccgagcccgc tctctgccga cagccccgcc ccggtcagga
     2881 tgacgatgtt gttatagcgc tccacggagg acggcagcta gcccttgccg gtctggccgc
     2941 ggtggcggag gtaatgatcg gcgagcacga tcgccatcat cgcctcgccg atcggcacgg
     3001 cgcggatgcc gacgcaagga tcatgacgcc ctttggtcga gatttcggtc tcgttgcccg
     3061 cggcatcgat ggtgcggcgc ggggtgagga tcgatgaggt cggcttcacc gcgaaccgcg
     3121 cgacgatggg ctggccggtg gagatgccgc cgagaatgcc gccggcattg ttggtcagaa
     3181 actcgggcgt gccgtcgcgg ccgatgcgca tctcgtcggc gttctcctcg ccggacagag
     3241 acgccgcggc gaagcctgcg ccgatctcga cgcccttcac cgcgttgatg ctcatcagcc
     3301 cgctagcgat atcctggtcg agcttgccgt agaccggcgc gccaagcccg gcagtcacgc
     3361 cttcggccac gatctcgatg atggcgccga cggaggagcc ctctttgcgc accttgtcga
     3421 ggaagtcgcc ccacttcttc accgccttgg cgtcgggaga gaagaacgga ttgcgctcga
     3481 cctcggacca atcccaggtg cggcggtcga tagcgtgggg accaacctgc accagcgcgc
     3541 cgcgcacgcg catgcccggc accactttgc gggctacggc gccagcggcc acgcggctcg
     3601 cggtctcgcg cgcgctcgcc cggcctgagc cgcgccaatc gcggacgccg tatttcatga
     3661 ggtaggtgta gtcggcatgg ccagggcgga acttgtcctt gatctcgctg tagtccttcg
     3721 accgtgcgtc catgttctcg atctcgagcg cgatcggcgt gccggtggtc acctgctcgc
     3781 cgctcgcctc gtcggtgaac acgccggaaa ggatacgcac ctggtcaggc tcctgacgct
     3841 gggtggtgta gcgcgactgg ccgggcttcc gcttgtcgag ataagtctgg atgtcggcga
     3901 cttcgagctg aatgcccgga gggcatccgt cgaccacgca gccgatcgca ggcccgtggc
     3961 tttcgcccca ggtggtgatg cggaaaagat ggccgaagct gttgtgcgac atggattgaa
     4021 cgtagccagc ttctagttaa ggccagggaa agggtcaaac gcctaaccga aaagcgccga
     4081 tctctacaac gtgtacatca gaaatattca acctatggta acataggcct cgcgctggtt
     4141 ttgcatccgt tgcgcaggat gcgtgtcccg gggaggtaca aatgttgcca acgaccttac
     4201 gcgatggcgc ctatgttccg aagcgagtga aggaggaaat cgatttcgac gggccgatcg
     4261 aacgcggcgc caagaagatg cgcgcgcaga gggtccagga atttctcacc ttgaacggct
     4321 tcgccgtctc gatcgacggc gattttggcc ctgggaccga aaagcgggtg cgagactttc
     4381 aggccgcgcg cggactgccc gccaccggcg tggtcgacat gaagacgcat gaggagccgg
     4441 tcgcgcccat cgtgaaggcc ttgaacccca tcgcggcagg gggaaagagc ctctccaccc
     4501 tcgccgcggc ctacgccaag caacacatca agaaccaccc aaaggaggct ggcgacgaca
     4561 atcggggccc ctgggtcagg agctatctcg attgggacgg aaaggacgca agatggtgcg
     4621 ccggcttcgt ctgctttgcg ctccagcagg cggcccacac gctcggcgtc aaccttccga
     4681 tcgactcctc atcgtcctgc gatgtgttgg cgatggaagc caagacggcc ggaaagttcg
     4741 tgccagaatc gaaggtgaag tcgggagcct tcgccaagaa ggaccttgtg ccaggcacct
     4801 tctttctcgt ccgcaaggtt cccggcgact gggtgcatgt cggcatcgtg accgagccag
     4861 ccaaggagtc gttcgacacc gccgagggaa acactggcag ctcgaacggc tttgaagcag
     4921 cggagcgggc gagagactac cggagcaagg actttatcgt ctggtaggcg ccttggacgc
     4981 ctctactcct tcgccacgga gatgtcaggg gacgtcctcg ttcttcatgc cgaccccagc
     5041 gcggtcacct gatctgcggg taatacccat cctgcgttga tctgtggcag gagccgcacc
     5101 gtcgcaaatc ggttagagtg aggccgaagg agacgtcgat gaaattaatg gtgctgctcg
     5161 cctcgcttgg tctggcgagt gctctcttgt tcgtttcggc tgacggcgct gatgccgtct
     5221 caagccgtca tcaagcggcc cacgctcggg cccatgccca gcattacgcc cgcaccaatc
     5281 gccacagccc ccggcgctac gccgcgcgcc gtggcggcta tcatgggaaa atgacgctaa
     5341 acgagatctt gtatgggccg gccggaatgc ctccagctcc ccatgatttc ggcccgcatt
     5401 tcgactatcc gccggagccg ctcaatggcg gcatcaccag ctcgccctac cttcactagt
     5461 cgcgctcttt cgggattggc aaacttgccc tgcgtgccgg aaggggcaac ccctactcct
     5521 tggccacgga aatgtcgggg gcgtcctggt tcttcatgcc gaccacgtgg tagccggcgt
     5581 cgatatgcag gatctcgccg gtgaccccac ggcccaagtc cgacaggaga tagagcgcgc
     5641 cgccgcccac gtcctcgatc gacacggtgc gcctaagcgg cgaattgtac tcgttccact
     5701 tgaggatgta gcggaagtcg gcgatgccgc ttgcggcaag ggtcttgatc ggcccggctg
     5761 agatggcgtt gacgcggatg ttctccttgc cgagatcggc cgcgagatag cgcacgcttg
     5821 cttctagcgc ggccttggcc acgcccatca cgttgtagtg cggcatcacc ttctcggcgc
     5881 cgtaatagct gagcgtgagc agagagccgc cattcggcat cagcctctcg gcgcgcttgg
     5941 cgagcgcggt gaacgagtag caggagacga gcagcgtctg ggtgaaattg tccttgctgg
     6001 tgtcgacata gcggccgtcg agctcttcct tgtcggagaa ggcgacggca tggacgagaa
     6061 aatcgatgct gccccaagag tcgccgagcg ccttgaacac cgcctcgacg cttccctcgt
     6121 cggtcacgtc gcaaggcagc acgagcttcg agccgatctc ggccgcgaga ggctcgactc
     6181 gcttcttgat ggcgtcgccc tggtaggtga gcgcgatctc cgcgccctgg tcgtagcagg
     6241 ccttggtgat gccccaggcg atggagcggt tgttggcgac gccgaacacg atgccgcgct
     6301 tgcccgccat caatttggca gcttccgcca ttgatcctcc agttccggaa atgacggcgc
     6361 tttatatgcg agaggcccct ggcccggaag gcttttccac gctgcttcat ccctccccta
//
LOCUS       DATNAE010000197        17582 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_4095939, whole genome shotgun sequence.
ACCESSION   DATNAE010000197 DATNAE010000000
VERSION     DATNAE010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 17582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17582
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4095939"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="VN518_04495"
     CDS             <1..218
                     /locus_tag="VN518_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXE03866.1"
                     /translation="PIAAFLARNPNGGIHHLCYEVPDIVEARDRLIAEGARVLGQGDP
                     KIGAHGKPVLFLHPKDFCGTLVELEQA"
     gene            222..497
                     /locus_tag="VN518_04500"
     CDS             222..497
                     /locus_tag="VN518_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018325956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1467 family protein"
                     /protein_id="HXE03867.1"
                     /translation="MALVFAISMYVVIWWIVLFAMLPIGVRTQAEEGEVSPGTPESAP
                     HRPRLLPKMLATTVVASIVFAAVYVIIVHRVITLDQIPFLPRYETVR"
     gene            1062..2408
                     /locus_tag="VN518_04505"
     CDS             1062..2408
                     /locus_tag="VN518_04505"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006934699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HXE03868.1"
                     /translation="MRLSRYFLPLLRDDPKEAEIVSHKLMLRAGLVRQSSAGIYSWLP
                     LGYRVLSKIEQIVREEQDRAGALELLMPTIQPAEIWRESGRYEVYGKEMLRISDRHER
                     EMLYGPTNEELITQIFRDGVKSYKELPRILYHIQWKFRDEIRPRFGVMRGREFLMKDS
                     YSFDLTLEDARISYNKMFVSYLRTFNRMGLKSIPMAAESGPIGGDMSHEFIILADTGE
                     SEVYCHRDLVTLEPPGADLDYDSDLTPIIQSWTGKYAATSEKHDAAGFAKEVPEVDRV
                     TARGIEVGHIFFFGTKYSESMGCKVQGPDGGLVAVQMGSYGIGVSRLVGAIIEASHDD
                     AGIIWPASVAPFDVGLISVKSGDAETDRVCEDLYAKLEAAGLSVLYDDRDERPGAKFA
                     AFDLIGLPWQLIVGPKSVKAGEVELKERATGARHTLSFDAALHRLTASAEPGRVAA"
     gene            2405..3685
                     /locus_tag="VN518_04510"
     CDS             2405..3685
                     /locus_tag="VN518_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HXE03869.1"
                     /translation="MSGNATRPNGTRAFSPFEWMVALRYLRARRREGFISVIAGFSFL
                     GIMLGVATLIIVMAVMNGFRQELFNKMLGLNGHVVVHSLDPNFGNYDAIAANVRGVHG
                     VKYALPLIEGQVMVSTPSSSTGALVRGLREADLKQLKAVSSNIRFGTLDGFDGKEGVA
                     VGSRLANDLNVKLGDQVTLLTPRGASTALGTAPRVKRYPIVAIFEIGMSEYDSSIMFM
                     PLAEAQRYFSKPDSVTVLEVVLDTPDEVSALGPKIIEAGGSSVYISDWRQRNATFFTA
                     LQVERNVMFLILTLIVLVAALNIISGLIMLVKDKGHDIAILRTMGATRAAVMRIFFIT
                     GASIGIVGTLAGLGLGVLVCLNIERIREFVGWITDTELFSPELYYLSQLPAEMDTGET
                     ASVVIMALVLSVLATLYPSWRASRLDPVEALRYE"
     gene            3694..4416
                     /locus_tag="VN518_04515"
     CDS             3694..4416
                     /locus_tag="VN518_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXE03870.1"
                     /translation="MGKSDQAPALHLDRLERAYAQGSRLIEVLKGATASLYPGEAVAL
                     LGPSGAGKSTLLHIAGLLERPDGGAVVIDGVDCVKLGDAERTRMRRSEIGFVYQFHHL
                     LPEFSALENVVLPQLILGLTRREAEERARQLLQALGLGERAEHRPAQLSGGEQQRVAI
                     ARAVANGPRLLLADEPTGNLDPHTAERVFDQLLSLVRESGVAALVATHNLDLAARMDR
                     VLRLMDGALVEEQVTVVRVSQA"
     gene            4585..8055
                     /gene="dnaE"
                     /locus_tag="VN518_04520"
     CDS             4585..8055
                     /gene="dnaE"
                     /locus_tag="VN518_04520"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HXE03871.1"
                     /translation="MSATEQPFVHLRVHSAYSLLEGALTIPHLVTLAAEQRMPALALT
                     DTNNLFGALEFSESLAQARIQPIVGCTLSLSFAPEAETQPENGELRRHVNHGRVALLA
                     KDAAGYANLIKLSTEAYRYGADSGDPLVTLSHLALHQAGLIALTGGPDGVIDRAIAAN
                     DFRFAESALNSLKSIFGDRLYVELQRHGLPQERTAEPVLLDLAYDNHLPIVATNEPYF
                     ASPDDFEAHDALICIAQGSYVAVDQRRRLSPEHYFKSARQMTELFADLPEAIENTIEI
                     ARRCSYRPREHAPILPQFVNGAASSVAQGGTEAAELRRQSEAGLARRLAEEGTAPGYD
                     AKAYSDRLAFELNVITGMNYQGYFLIVADFIQWAKKEGIPVGPGRGSGAGSLVAWALT
                     ITDLDPMRFGLLFERFLNPERVSMPDFDIDFCQDRRDEVIAYVRERYGADRVAHIITF
                     GKLQARAVLRDVGRVLQMPYGQVDRLCKLVPHNPANPVTLSQAIAGEPRLQEARDSEP
                     IVAKLLDIGQRLEGLYRHASTHAAGVVIADRPLIELVPLYRDPRAQLPATQFNMKWAE
                     AAGLVKFDFLGLKTLTVIETARELLARKGVALDPARLPLDDAATYELLARGDTVGVFQ
                     LEGAGMRDALRKLKPDRFEDIIAIVALYRPGPMDNIDSYVNRKHGREQPDYLHPLIQP
                     ILEETYGVIIYQEQVMQIAQTLSGFSLGEADLLRRAMGKKIKNEMAQQRNRFIEGAVG
                     NGVDRGSAEFIFELVAKFAGYGFNKSHAAAYALIAYQTAYLKANHPIEFLAASMTLDI
                     GNTDKLNGFAQEARRMGIRIEPPSVNRSEAGFMPSNGAIRYSLAALRNVGRHAVDHIC
                     AERAKRGSFKDVSEFARAINPRLVNKRALEMLAAAGAFDELGIDRATALANVDRIMAA
                     GNRSLADETGGQNDLFAGAARTPPPIELRAAKPWVPTDRLSHELEAVGFFLTGHPLDD
                     YHDVLEALGAESWAEFAAKARMRRVVGTLAGTVLATRQRQGKSGNAYAFVAFSDPTGQ
                     FEAVVFSEALAASRELLQPGSAVLLDVEAEADGETVKARVQRITSLEAAAEARHSGIK
                     IYLEGTRALDSIAEQVGDRGGQGELRLVLRLDDAGREVEFVLPQGIDATPKQRSALKL
                     VEGVAAVSAL"
     gene            8240..8998
                     /gene="rpsB"
                     /locus_tag="VN518_04525"
                     /pseudo
     CDS             8240..8998
                     /gene="rpsB"
                     /locus_tag="VN518_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334544.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
     gene            9224..10153
                     /gene="tsf"
                     /locus_tag="VN518_04530"
     CDS             9224..10153
                     /gene="tsf"
                     /locus_tag="VN518_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HXE03872.1"
                     /translation="MTAINATMVKDLREKTGAGMMDCKAALNETAGDMEAAVDWLRTK
                     GLAKAAKKAGRVAAEGLIGLAANDKEAGLVEVNSETDFVARNDKFQEMVRTIAADALK
                     AKGDLDKLAKGKYGGGKLSVTDHLKEMIGSIGENMTLRRAAYIGVKTGAVASYMHNTI
                     SPGLGKIGVIVALESTGDAEKLKSFGKQVAMHIAAANPQSVDVAGLDPAFVERERAVL
                     TEQAKESGKPAAVIEKMVEGRLRKFYEEVVLLSQAFVHDPETTVAKALAATEESAGAP
                     IKIAGFHRFALGEGIDRPDSDFAAEVAAAASGS"
     gene            10175..10906
                     /gene="pyrH"
                     /locus_tag="VN518_04535"
     CDS             10175..10906
                     /gene="pyrH"
                     /locus_tag="VN518_04535"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HXE03873.1"
                     /translation="MAQPNTGRSLRYRRVLVKLSGEALMGKNGYGIDVEVLNQVAADI
                     CEAADLGLELCLVVGGGNIYRGLAAATDGMDRVSGDYMGMLATVLNGLALGQALEAKG
                     RSARVMSAIPMISICEPFIRARALAHLADGHLLVLVGGTGNPFFTTDTTAALRAAEMG
                     CDAIFKATQVDGVYDSDPAKNPKARRYEALSYDDVLRQDLKIMDGAAIALARDNRIPI
                     IVFSIKEKGGLAAVLQGKGSSTTIS"
     gene            10996..11553
                     /gene="frr"
                     /locus_tag="VN518_04540"
     CDS             10996..11553
                     /gene="frr"
                     /locus_tag="VN518_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HXE03874.1"
                     /translation="MAGFDLKEIERRMRGALAVLKQEFGGLRTGRANMSLLDPIMVNA
                     YSSQMPLNQLATINVPESRLITVQVWDRSQVGAVERAIRESDLGLNPVVEGQLLRLPI
                     PELNEERRHEIAKVAHKYTEQARVAVRNVRRDGMEHLKRMEKDGDMGKDEHHSMSTKV
                     QELTDKIIKEIDETLASKEAEIMQV"
     gene            11611..12369
                     /locus_tag="VN518_04545"
     CDS             11611..12369
                     /locus_tag="VN518_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HXE03875.1"
                     /translation="MTLAVEELGWSETEVPVPRHIAIIMDGNGRWAAERGLPRAEGHR
                     QGVDSVRRSVKAALELGITHLTLFSFSSENWSRPRQEIHDLFGLMRRFVRRDLADLHK
                     NGVRIRVIGTRSGLDDDIMRLIDDAVLLTKDNSALNLTIAFNYGSRDEIARAAARVAE
                     AVAEGTLDVSDITPERFGSYLDTADLPDPDLLIRTSGELRLSNFLLWQLAYSEFVFVD
                     TYWPDFSREALEAAIAEYQRRTRRFGGLKARSTA"
     gene            12366..13226
                     /locus_tag="VN518_04550"
     CDS             12366..13226
                     /locus_tag="VN518_04550"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HXE03876.1"
                     /translation="MTPLDVTPSGAEPRAQPNKSEMTTRLTSAVVMAALALGSVVVSI
                     WTFVALAIASGVIVSWEWGRLVRKAGFDAIAMMQSAAVAAVAVTVAVSRPGLALIATA
                     LALAGTGLAAASSPTLGWSLLGIAYSVLPAWSMVWLRSDQTLGVTALLYLLAVAWTTD
                     TVSYAGGRLIGGPKLAPQISPRKTWSGLLVGTFAPALIGFAFAWFLGETSGWRLALVS
                     VAIGAACQIGDLSESAAKRRFGAKDMSNLIPGHGGLLDRIDGLLIAAIAAALIALRDP
                     ANPGRGLLIW"
     gene            13237..14475
                     /gene="dxr"
                     /locus_tag="VN518_04555"
     CDS             13237..14475
                     /gene="dxr"
                     /locus_tag="VN518_04555"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HXE03877.1"
                     /translation="MTVLAVQSQTVRLASPRARPRRISVLGATGSIGENTLDLVGRDP
                     ASFTVVALTGGRNAARLAELAVKHRAELAVIADETCYAELRARLSGTGIEAAAGEDAL
                     VEAASRPADWVMAAIVGAAGLKPTLAAVRQGTCTALANKECLVSAGPIFMREVAKYRT
                     ALLPVDSEHSAAMQVMAGGAPESIERICLTASGGPFRSWSLADMASVTPKQALAHPNW
                     SMGPKVTIDSATLMNKALELIEAHHLFSVPAERLSVLIHPQSIVHCLVHYRDGSVLAQ
                     MSCPDMRTPIAYSLAWPNRMQAPTARLDLASLGALSFEAPDEERFPALRLARQVLAAG
                     GGSSTVLNAANEVAVQAFLDGRIGFLGIAALVEATLGRSAHISAPSPDSVDEVLAVDA
                     EARAIALSQLLRFAQSAQAR"
     gene            14502..15644
                     /gene="rseP"
                     /locus_tag="VN518_04560"
     CDS             14502..15644
                     /gene="rseP"
                     /locus_tag="VN518_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HXE03878.1"
                     /translation="METILKLSGFGDTVLSYLLPFLFVLTIVVFFHELGHFLMARWCG
                     VAVKTFSIGFGPEIVGFNDRHGTRWRLSWIPLGGYVKFIDDENAASAGQRPLEALNEE
                     ERKTSFQGKSLGARAAIVAAGPIANFVLAIVIFTAIFTFFGERSTAAKVDIVNPGSSA
                     ERAGFKTGDLVVKIDGTTIENFGQMQRIVGMSPNQQLHFEVERGGKPVELTATPELKE
                     ITDNFGNTMRIGLLGIQRSASPDDWTLKRHDPLTAFVMAVKECYFVISRSLGYLYDVI
                     TGREAADQLGGPIRIAQVSGQVATAGFFALLNLAAIISVSIGLINLFPIPMLDGGHLL
                     FYGIEAVRGKPLSENIQEIGFRIGLAFVLMLMIFATWNDLIHLKFL"
     gene            15812..>17582
                     /gene="bamA"
                     /locus_tag="VN518_04565"
     CDS             15812..>17582
                     /gene="bamA"
                     /locus_tag="VN518_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HXE03879.1"
                     /translation="MARGLSAALLAAGAIVLSIGMSASLGELSAYAQAGPAVGAIRVE
                     GNKRVEPETVRTYLTFNVGDPYDLAKVDESLKALFATGLFQDVRIRREGSTVVIVVVE
                     NPIVSRVAFEGNKEVEDDALTAEVQLKPRTVYTRARVQADVQRILDVYRRQGLYAAQV
                     DPKIIKLENNRIDVVFEINEGPTTKVRAINFIGNTAFSDSQLRYVISTTQTTLLSFLK
                     STNIYDPDRLNLDRELLRQFYLKNGFADVRVISATADLDRDGRGFFITFTLDEGPKYR
                     FGDIDVETTLPSLDVRTLRRSLVTRTGRVYNVQRIESTAEALTIISSQQGYAFAQVKP
                     RFDRDEVNRRINITYVIEEGPHIYIERINIVGNFRTHDDVIRREFSLAEGDAFNRLLV
                     EAARRRLRSLGFFKTVDIDTEPGSAPDRVVLNVTVQEQPTGELSFGAGYSTSEGVIGD
                     ISITERNLMGRGQYVRLGFSGSLQRAQVDFSFTEPHFLDRNLAAGFDLFHKEVDFTNV
                     ASYRERDTGGNLRMGFPIAFNTQLGLRYRLEREEIYDVADNASLAVKEAEGESIVSSV
                     GFTVAYDTRNLPQSPTSGIFSSFS"
BASE COUNT         3171 a         5833 c         5430 g         3148 t
ORIGIN      
        1 ccccgatcgc tgccttcctg gcgcgcaatc ccaatggcgg catccaccat ctctgctacg
       61 aggtgcctga catcgtcgag gcgcgcgacc ggctcatcgc cgaaggcgcc cgcgtgctgg
      121 gccaaggcga ccccaagatc ggcgctcatg gcaagccggt gctcttcctc catcccaagg
      181 atttctgcgg cacgctggtg gagctcgagc aggcgtaagc catggcgctc gtattcgcca
      241 tctccatgta cgtcgtgatc tggtggatcg tgctgttcgc catgctgccg atcggcgtgc
      301 gcacccaggc cgaggaaggc gaggtttctc ccggcacgcc ggaaagcgcg ccgcaccggc
      361 cgagactgct gccaaagatg ctggccacca cggtggtcgc gagcatcgtg ttcgcggcgg
      421 tctatgtgat catcgtccat cgcgtgatca cgctcgacca gattccgttc ctgccgcgct
      481 acgaaacagt gcgctgagcg cgcccgagag gcgtgcgcac aacaaaaaaa gcaaggtcaa
      541 acgaccttgc tttctttgtc gtctcaatac tccttatctc accggcctgg acgaggctcg
      601 ccgtcagtct tgactgtcga gtattgctgg ctggatgagc ggctgcgcct gctttgctgc
      661 ttatcagcgc gcctggagct acttcctccc aagacttgga cctggtccaa tctcaacgcg
      721 tgactccgag atcactaccg gtcccgaacc ctcatcacgt tgtgccaggg acactagcaa
      781 ggccgcgcca aattgtcacc atgattgtga ctgaacggca taatttcatg ccttcgcccc
      841 cgttcggcgt aggcagatcg cctcaaaatt agtcagatcg agaaattcgt ggcctcatcg
      901 ccacgtcctg ttgcacgacc caatcggtaa gcggccgacg cgattggcaa cgtctggtgg
      961 agagagcggg ccgcccctcc cgcgattgct aacccgcgcc aaagtaagct atagccgcga
     1021 aacggtttcg ccagccctac aagccacgag cacggctcct catgcgcctc agccgctatt
     1081 tcctgcccct gttgcgcgac gacccgaagg aagccgagat cgtctcccat aagcttatgc
     1141 tgcgtgccgg cctcgtccgc caatcgagcg ccggcatcta ttcctggctg cccctcggct
     1201 atcgggtgtt aagcaagatc gaacaaatcg tgcgcgagga gcaggaccgc gccggcgcgc
     1261 tcgaattgct catgccgacc atccagccgg ccgagatctg gcgggaaagc ggccgctatg
     1321 aggtctacgg caaggagatg ctgcgcatca gcgaccgcca tgagcgcgag atgctttacg
     1381 ggcccaccaa cgaggagctg atcacccaga tcttccgcga cggcgtgaag tcctacaagg
     1441 agctgccgcg catcctctat cacatccagt ggaaattccg cgacgagatt aggccgcgct
     1501 ttggcgtgat gcgcgggcgc gagttcctga tgaaggattc ctactccttc gatctcaccc
     1561 tcgaggacgc gcgcatctcc tacaacaaga tgttcgtctc ctacctgcgg accttcaacc
     1621 gcatggggct gaaatcgatc cccatggccg ccgaatcggg gccgatcggc ggcgacatga
     1681 gccacgagtt catcatcctc gccgacaccg gcgagagcga agtctattgc caccgcgatc
     1741 tcgtcacgct ggagccgccg ggggccgacc tcgactacga cagcgatctc actccaatta
     1801 ttcagtcgtg gaccggcaaa tacgcggcca ccagcgagaa gcacgatgcc gccggttttg
     1861 ccaaggaggt gcctgaagtt gaccgcgtga ccgcccgcgg catcgaggtc ggccatattt
     1921 tcttcttcgg caccaagtac tccgaatcca tgggatgcaa ggttcagggc cccgatgggg
     1981 gcttggtggc ggtgcagatg gggtcctacg ggatcggcgt gtcgcggctc gtcggggcca
     2041 tcatcgaggc gtcgcatgac gatgccggca tcatctggcc ggcgagcgtc gccccgttcg
     2101 acgtcggcct gatcagcgtg aagagcggcg atgccgagac cgacagggtc tgcgaggatc
     2161 tttatgccaa gctcgaggcg gccggcttga gcgtcctcta cgacgaccgc gacgagcggc
     2221 ccggcgccaa attcgcggcc ttcgacctga tcgggcttcc ttggcaactg atcgtgggac
     2281 caaagagcgt caaagccggc gaggtcgagt tgaaggagcg cgccaccggg gccaggcaca
     2341 cgctcagctt cgatgcggcc ttgcatcgcc tcaccgcctc ggccgagcct ggcagggtag
     2401 cggcatgagc ggcaacgcca cgcgccccaa cggcacaagg gccttctcgc ccttcgaatg
     2461 gatggtggcg ctccgctatt tgcgcgcccg ccggcgggag ggcttcatct cggtgatcgc
     2521 cggcttctcc ttcctcggca tcatgctcgg cgtcgccacg ctgatcatcg tcatggcggt
     2581 gatgaatggc ttccgccaag aactgttcaa caagatgctg gggttgaacg gccatgtcgt
     2641 ggtgcacagc ctcgatccca atttcggcaa ttacgacgcc attgccgcca atgtgcgtgg
     2701 cgtgcacggc gtcaaatacg ccctgccgct gatcgagggc caggtgatgg tgtcgacgcc
     2761 gtcatcctcc accggggcac tggtgcgcgg cttgcgcgag gccgacctga agcagctgaa
     2821 ggccgtttcc agcaatatcc gcttcggcac cctcgatggc tttgacggca aggaaggcgt
     2881 ggcggtgggc tcgcggctcg ccaacgacct caacgtcaag ctcggcgatc aggtgacgct
     2941 gctcaccccg cgcggcgcct cgaccgcgct cggcacggcg ccgcgggtga aacgttaccc
     3001 gatcgtcgcc atctttgaga tcggcatgtc ggagtatgat tcgagcatca tgttcatgcc
     3061 gcttgccgag gcgcagcgct atttctccaa gcctgacagc gtcaccgtgc tcgaagtcgt
     3121 gctcgatacg ccggacgagg tgagtgcgct cgggccgaag atcatcgagg ccggcggctc
     3181 ctcggtctat atcagcgact ggcggcagcg gaacgcgacc ttcttcaccg cgcttcaggt
     3241 cgagcgcaac gtcatgttcc tgattctcac actgatcgtg ctcgtggccg cgctcaacat
     3301 catctcgggc ctgatcatgc tggtgaagga caagggccac gacattgcca tcctgcgcac
     3361 catgggggcg acccgtgccg cggtgatgcg catcttcttc atcaccggcg ccagcatcgg
     3421 catcgtcggt acgctcgccg gcctcgggct cggcgttctt gtctgcctca acatcgagcg
     3481 aatccgcgag ttcgtcggtt ggatcaccga caccgagctg ttctcgcctg agctctacta
     3541 cctgtcgcag ttgccggccg agatggacac tggcgagacc gcctcggtgg tgatcatggc
     3601 gctcgtgttg tcggtcctcg ccacgctcta tccctcctgg agagcctctc gtctcgatcc
     3661 cgtcgaagcc ttgcgctacg agtagggcgg gtcgtgggaa aatcagatca ggctccggcg
     3721 ctgcatctcg accggctgga gcgcgcttac gcccaaggct cccgcctcat cgaggtgctg
     3781 aagggcgcga ccgcgtctct ttatcctggc gaggcggtgg cgctgctcgg gccctccggc
     3841 gccggcaagt ccaccttgct ccatatcgcc ggcctcctcg agcggcctga cggcggcgct
     3901 gtggtgatcg acggcgtcga ttgcgtcaag ctcggcgacg ccgagcgcac gcgcatgcgc
     3961 cgctccgaga tcggcttcgt ctatcagttc caccaccttc tgcccgagtt ctcggcgctg
     4021 gagaacgtgg tgctgccgca gctcattctc gggctcacgc ggcgcgaggc cgaggagcgc
     4081 gccaggcaat tgttgcaggc gcttggcctc ggcgagcggg ccgagcatcg tccggcacaa
     4141 ctgtccggcg gcgagcagca gcgcgtcgcc atcgcccgcg ccgtcgccaa cggaccgagg
     4201 ctgctcctcg ccgacgagcc gaccggcaat ctcgatccgc atacggccga gcgcgtgttc
     4261 gatcagctct tgagcctcgt gcgcgagtca ggggtcgcgg cgctggtcgc cacccacaat
     4321 ctcgaccttg ccgcccgcat ggaccgcgtc ctgcgcctca tggacggcgc cctggtcgag
     4381 gagcaggtga ccgtggtgcg ggtctcccag gcctagccgg attggcggga cgtcttggca
     4441 aggcacactt gcttcattcc tttaaatgga acaaaataag aacatctgtt gaaaagtccg
     4501 atagttcggg cggcgggttt gcccggccac tgcgagtcgc ggagggttgg ttgtgagtac
     4561 cgaacgtgag gagagacgct agccttgtcc gcgaccgagc aacccttcgt gcatcttcgc
     4621 gtgcactcag cctattcgct gctcgaaggg gctctgacca ttccccatct cgtcacgctt
     4681 gccgccgagc agcgcatgcc ggcgcttgcg ctcaccgaca ccaacaatct gtttggcgcg
     4741 ctcgaattct ccgagagttt ggcccaggcc cgcatccagc cgatcgtcgg ctgcacttta
     4801 tcgctgagct ttgcaccaga agcggagact cagccggaaa acggtgagct gagacggcat
     4861 gtaaatcatg gccgcgtagc actcctcgcc aaggacgcgg ccggctacgc caatctcatc
     4921 aagctctcga ccgaggccta tcgctatggc gcggacagcg gcgaccctct cgtcacgctc
     4981 tcccatcttg ccctccacca ggcggggctt atcgcgctca ccggcggtcc tgacggcgtc
     5041 atcgaccgcg ccatcgccgc caacgatttc aggtttgccg agtctgctct caattcactc
     5101 aaaagcattt tcggcgaccg cctctatgtg gagctgcagc ggcatgggct gccccaggag
     5161 aggacggcag agccggttct gctcgatctc gcctatgaca accatctgcc gatcgtcgcc
     5221 accaacgagc cttacttcgc gagccccgac gatttcgagg cgcatgacgc gctgatctgc
     5281 atcgcccaag gaagctatgt cgcggtggac cagcggcggc gcctgagccc cgagcattat
     5341 ttcaagagcg cgcggcaaat gaccgagctg ttcgccgatc tgccggaggc gatcgagaac
     5401 accatcgaga tcgcccgccg ctgctcctac cgcccgcgcg agcatgcgcc gatcctgccg
     5461 caattcgtga atggcgcggc ttcgtccgtg gcgcaaggcg ggacggaagc ggccgagctt
     5521 cgccgccagt cggaggcggg tctggcgagg aggcttgccg aggaggggac ggcgcctggt
     5581 tatgacgcca aggcttattc cgatcgcctc gccttcgagc tcaacgtcat caccggcatg
     5641 aactatcagg gctatttctt gatcgtcgcc gacttcatcc aatgggccaa gaaggagggc
     5701 attccggtcg ggccaggacg aggctcaggc gccggctcgc ttgtcgcctg ggcgctgact
     5761 atcaccgatc tcgaccccat gcgctttggg ctgctgttcg agcgcttctt gaaccctgag
     5821 cgcgtgtcga tgccggactt cgacatcgac ttctgccagg accgccgcga cgaggtgatc
     5881 gcctatgtgc gcgagcgtta cggcgccgac cgcgtcgccc acatcatcac cttcggtaaa
     5941 ctgcaggcgc gcgccgtgct tcgcgacgtc ggccgcgtgc tgcagatgcc ctatggccag
     6001 gtcgaccgcc tgtgcaagct cgtgccgcac aatccggcga acccggtcac gctctcgcaa
     6061 gccattgccg gcgagccgcg gctacaggag gcgcgcgact cagagccgat cgtggccaag
     6121 ctcctcgata tcggccagcg cctcgaggga ctttatcgcc acgcctcgac ccatgccgcc
     6181 ggcgtggtga tcgccgaccg gccgctgatc gagcttgtgc cgctctaccg cgacccgcgc
     6241 gcccagcttc ccgccaccca gttcaacatg aaatgggccg aggccgccgg gctcgtgaag
     6301 ttcgactttc ttgggcttaa aacgctgacc gtgatcgaga ccgcgcgcga gctgctcgcg
     6361 cgcaagggcg tcgcgctcga tccggcgcgg ctgcccctcg acgacgctgc gacctacgag
     6421 cttcttgcgc gcggcgatac ggtcggcgtg ttccagctcg aaggtgcagg catgcgcgac
     6481 gctctgcgca agctgaagcc cgaccgcttc gaagacatca tcgccatcgt ggcgctttat
     6541 cgcccggggc cgatggacaa catcgacagc tacgtgaacc gcaagcacgg gcgcgagcag
     6601 cccgactacc tgcacccgct gatccagccg attctggaag agacctatgg cgtgatcatc
     6661 taccaggagc aggtgatgca gatcgcgcag accctctcag gcttctcgct cggcgaggcc
     6721 gatctgctcc gccgcgccat gggcaagaag atcaagaatg agatggcaca gcagcggaac
     6781 cgcttcatcg agggcgcggt cggcaatggc gtggatcgcg gcagcgccga gttcatcttc
     6841 gagctggtcg ccaagttcgc cggctacggc ttcaacaagt cccatgccgc ggcctacgcg
     6901 ctgatcgcct atcagaccgc ctatctcaag gccaaccacc cgatcgagtt cctggcggcc
     6961 tccatgacgc tcgacatcgg caacaccgac aagctcaacg gcttcgccca ggaagcccgc
     7021 cgcatgggca ttcgcatcga gccgccgtcg gtgaaccgct ccgaggcagg ctttatgccg
     7081 agcaacggcg ccatccgcta ttcgcttgcg gcgctgcgca atgtcggccg ccacgccgtc
     7141 gaccacattt gcgccgagcg agcgaagcgc ggcagcttca aggacgtctc cgaattcgcc
     7201 cgcgccatca atccgcgcct cgtcaacaag cgtgcgctgg agatgctcgc cgccgccggc
     7261 gcgttcgatg agcttggcat cgaccgggcc acggcgctcg ccaatgtcga tcgcatcatg
     7321 gccgctggca accgatcgct tgcggacgag accggcggac agaacgacct cttcgccggt
     7381 gccgcgcgca cgccgccgcc gatcgagctc cgcgccgcca agccttgggt ccccaccgac
     7441 cggctatcgc atgagcttga ggcggtgggc ttcttcctca ccggccatcc gctcgacgac
     7501 tatcatgacg tgctcgaagc gcttggcgcc gagtcctggg cggaattcgc ggcgaaggcg
     7561 cgcatgaggc gcgtcgtcgg cacgctcgcc ggaacagtgc tcgccacgcg ccagcgtcag
     7621 ggcaagagcg gaaacgctta cgccttcgtc gccttctccg acccgaccgg ccagttcgag
     7681 gcggtggtgt tctcggaagc cttggccgcc tcgcgcgagc tgctccagcc gggcagcgcc
     7741 gtgctgctcg acgtcgaggc cgaagccgac ggcgagaccg tcaaggcgcg ggtccagcgc
     7801 atcacttcgc tcgaggcagc ggccgaggcc cgccatagcg gcatcaagat ttatctcgag
     7861 ggcacccgcg cgctcgatag catcgccgag caggtcggcg accggggcgg ccagggcgag
     7921 ctgaggctgg tgcttcgtct cgacgatgcc ggacgcgagg tcgagtttgt gctgccgcaa
     7981 ggcatcgacg ccacgccgaa gcaacggagc gctctcaagc tggttgaggg cgtggccgcg
     8041 gtcagcgccc tctgaccggc ttgaacccag ggccgcaacc cttgccaaga gggggttgca
     8101 tccctataaa ccgcaacatc tcacacgcgg gatctcagcg ccgcgaggcc cttaagggct
     8161 ctcacgctcg ctccggtgac cgggaaaccg gtctcatcct cccgcggcgg cagaaccgga
     8221 aaaaggagaa tgcagtccca tggcactgcc taatgtttct atgcgccaac tgcttgaggc
     8281 tggcgtgcat tttggtcacc agacccaccg ctggaacccg aagatggcgc cgtttatcta
     8341 cggctcccgc aacaacatac atatcatcga cctcacccag accgtgcccc tgtttcatca
     8401 ggcgctgctc gccgtggccg acaccgtggc caaaggcggc cgcgttctct tcgtcggaac
     8461 caagcggcag gcctccgagg ccgtggcgga cgctgcgcga tcttgcgccc agtattacgt
     8521 gaaccaccgc tggctcggcg gcacgctcac caactggcgc accatctcga actcgatccg
     8581 caggctcaag tccctggaag agctgcttgc tggcgagacg caaggcctga ccaagaagga
     8641 gctgttgcag ctcacccgcg agaaggacaa gctcgagcgg gcgcttggcg gcatcaagga
     8701 catgggcgcc cctcctgacc tcatgttcgt gatcgacacc aacaaggaag cgatcgccat
     8761 cgccgaggca cgcaagctca acatgccgat cgttgccgtg gtcgattcga acagcaatcc
     8821 tgatgggatc agtcatccca ttcccggcaa cgacgacgcc ggccgcgcca tcacccttta
     8881 ttgcgacatg atcgcgcgtg ccgccatcga cggcatcgag cgggcgcaag gctcagctgg
     8941 ggtggacctc ggcgccgccg acacgcctgc gggcgagccc gcacttgatg agggagagga
     9001 aggcggggag ggggccgagc gtcctcagac cgacggcgag cgcaggcccc agcggcggag
     9061 gccgccggga cggcccgacg caagagccgg caacggcaag ggcccgtcgc aggcaaggcg
     9121 ccccgcaagg ggaccgaggc cgagcgccga agccaagcct gaatagactg acgatcaaag
     9181 tttagggccg gggctaacca gttccggccc gatagaggat ggcatgacag cgattaatgc
     9241 aacgatggtg aaggacctgc gcgagaagac cggcgcgggc atgatggatt gcaaggccgc
     9301 gctcaacgag accgctggcg acatggaggc tgcggtcgat tggctgcgca ccaaaggtct
     9361 tgccaaggcc gccaagaagg cgggccgcgt ggcggctgag ggcctgatcg gccttgccgc
     9421 caacgacaaa gaggctggac tggtcgaggt caactccgag accgatttcg tcgcccgcaa
     9481 cgacaagttt caggaaatgg tgcgcaccat cgctgccgac gcgctcaaag ccaagggcga
     9541 cctcgacaag ctcgccaagg gcaagtatgg cggcggcaag ctcagcgtga ccgatcacct
     9601 gaaggagatg atcggctcga tcggcgagaa catgacgctc cgtcgcgccg cctatatagg
     9661 cgtgaagacc ggcgcggtgg cgagctacat gcacaacacc atttcgccgg gccttggcaa
     9721 gatcggcgtc attgtcgcgc tcgaatccac cggcgacgcc gaaaagctca agagcttcgg
     9781 caagcaggtg gcaatgcaca tcgccgccgc caatccacaa agcgtggacg tggccggtct
     9841 cgatccggct ttcgtcgagc gagagcgggc ggtgctcacc gagcaggcga aagagtccgg
     9901 caagccggcg gcggtgatcg agaagatggt cgaaggccgc ttgcgcaaat tctacgaaga
     9961 ggtcgttctc ctgtcacagg ccttcgtcca cgatccggag acgaccgtcg ccaaggcgct
    10021 tgccgcgacc gaagagagcg ccggcgcgcc gattaagatc gccggcttcc atcgcttcgc
    10081 ccttggcgaa ggcatcgacc ggccggacag tgatttcgcc gcggaggttg ccgcggcagc
    10141 gtctggatcc tagaggcagg gcgggggatc gctgatggca caaccaaata cgggccgatc
    10201 gctccgctac cgccgggttc tcgtcaagct ctccggcgag gccctgatgg gaaagaacgg
    10261 ctacggcatc gacgtggagg tgctgaacca ggtcgccgcc gatatctgtg aggccgccga
    10321 tcttggcctc gagctctgcc tcgtggtcgg cggcggcaac atctatcgcg gcctcgccgc
    10381 cgccaccgac ggcatggacc gggtgagcgg cgattacatg ggcatgctgg ccacggtgtt
    10441 gaacggcctc gctctcggcc aggcgcttga agccaaaggc cggtcggcgc gggtcatgtc
    10501 ggccattccc atgatcagca tctgcgagcc cttcatcagg gccagggcgc ttgcccattt
    10561 ggcagacgga catctgctcg ttttggtcgg agggaccggc aacccgtttt tcaccaccga
    10621 caccacggcg gcgctgcggg cggcagaaat gggctgcgat gcgatcttca aggccaccca
    10681 agtcgacggc gtctatgact ccgacccggc caagaacccc aaagcgcgcc gctacgaggc
    10741 actaagctac gacgatgtgc tgcggcagga tctcaagatc atggacgggg cggcaatcgc
    10801 ccttgcccgc gacaatcgga ttccgataat tgtgttctcc atcaaggaaa aaggcggttt
    10861 ggctgccgtc ttgcaaggga aggggtcctc gaccacgata tcctagcccg ccgcaacccg
    10921 gcgacttcgg gcggatgggc gggatcacaa gctgacccaa gcgcggattt caaccgcgcg
    10981 aggaggaaag gcgacatggc tggcttcgac cttaaggaga tcgagcggcg gatgcgtggc
    11041 gcgctcgccg tgctcaagca ggagttcggc ggcttgcgca cgggccgagc caatatgagc
    11101 ctgctcgatc cgatcatggt aaatgcctat agcagccaaa tgccgctcaa tcagctcgcc
    11161 accatcaacg tgccggaatc gaggctgatc accgtgcagg tgtgggaccg gtcacaggtc
    11221 ggcgctgtgg agcgcgccat ccgcgaatcc gatctcgggc tgaaccctgt ggtcgaagga
    11281 cagctgcttc gcctgccgat ccccgagctg aacgaggagc gccgccacga gatcgcgaag
    11341 gtcgcgcaca aatacaccga gcaggcgcgc gtcgcggtgc gtaacgttcg ccgcgacggc
    11401 atggagcacc tgaagcgcat ggagaaggac ggcgacatgg gcaaggacga gcatcactcg
    11461 atgtccacca aggtgcagga actgaccgac aagatcatca aggaaatcga cgagacgctc
    11521 gcgagcaagg aagccgagat tatgcaggtc tagccggcga gccggcgcca agagcacaat
    11581 gcctgtcact cttattggag caccgaacac gtgacgcttg cagtagagga gcttggctgg
    11641 tctgagaccg aggtaccggt acctcggcac atcgccatca ttatggatgg caatggccgc
    11701 tgggcagcgg agcggggcct gccgcgcgcc gagggtcatc gccagggcgt ggatagcgtg
    11761 cggcgctcgg tcaaggctgc gctcgagctc ggcatcaccc atctcacgct attcagcttc
    11821 tcgtcggaga attggtcgcg ccctcggcag gagatccacg atctgttcgg cctcatgcgg
    11881 cgcttcgtca ggcgcgacct cgccgatctg cataagaatg gcgtgaggat cagagtgatc
    11941 ggcacccgct caggcctcga tgacgacatt atgcggctga tcgacgacgc cgttctcctc
    12001 accaaggaca attcggcgct taatctcacc atcgccttca actatggctc cagggacgag
    12061 atcgcccgcg ccgctgcgcg cgtcgccgag gccgtggctg aaggcacgct ggatgtgtcc
    12121 gatatcacgc cagagcggtt cggctcctat ctcgacaccg ccgatttgcc cgatccagac
    12181 ttgctgatcc gcacgagcgg cgagttgcgc ttgagcaatt tcctgctgtg gcagctcgcc
    12241 tatagcgagt tcgtcttcgt cgatacctat tggcccgact tctcgcgcga ggctctcgaa
    12301 gccgcgattg ccgagtatca gcggcgcacg cgccgcttcg gcggccttaa ggcgagatcc
    12361 accgcgtgac gccgctcgat gtgacgccga gcggcgccga gccccgcgct caacccaaca
    12421 aatcggagat gacaacgcgg cttacatccg ccgtggtgat ggcggcgctg gcgctcggct
    12481 ccgtggtggt cagcatctgg accttcgtgg cgctcgccat agcctcaggc gtcatcgtct
    12541 cctgggaatg gggacggctg gtgcggaagg ccggcttcga tgccatcgcc atgatgcaat
    12601 ccgcggccgt cgccgccgtc gccgtgactg tggctgtgtc caggcccggt cttgctctca
    12661 ttgccacagc gctcgcgctc gccgggaccg gacttgccgc cgcctcgtcc cccacgcttg
    12721 gctggtcgct gctcggcatc gcctattcgg tgctcccggc ctggtccatg gtgtggctga
    12781 gaagcgacca gaccctgggc gtcaccgcgc tgctttatct gctggccgtg gcctggacca
    12841 ccgacaccgt ttcctatgcc gggggccggc tgatcggcgg cccgaaactc gccccgcaaa
    12901 tctcgccgcg aaagacctgg agcgggcttc tggttggcac ctttgcgccg gcgctgatag
    12961 gatttgcttt tgcctggttt ttgggggaga cctcgggctg gcgcctggcc ctggtcagcg
    13021 tggccatcgg cgcggcgtgt cagatcggcg atctgagcga gagcgcggcg aagcgccggt
    13081 ttggagccaa ggacatgagc aatctcatcc ccggccacgg tgggttgctc gaccgcatcg
    13141 atggccttct catcgccgca atcgcggcgg cattgatcgc cttgcgcgat ccggccaatc
    13201 ccggccgcgg cctgctgatc tggtgaggcg ttgcgtatga ccgtgctcgc cgttcaatca
    13261 caaaccgtca ggctcgcatc gcctcgcgcg cgcccgcggc gcatctcggt gctcggcgcc
    13321 accggatcga tcggcgagaa cacgctcgat ctcgtcggcc gtgatccagc gtcctttacc
    13381 gtggtggcgt tgaccggagg ccgcaacgcc gcgcgcctcg ccgagcttgc cgtgaagcat
    13441 cgggccgagc ttgcggttat tgccgacgag acctgctacg ccgagcttcg cgcccggctg
    13501 agcggcaccg gcatcgaggc cgctgctggc gaagacgcgc tggtcgaagc ggcaagccgt
    13561 cctgccgatt gggtaatggc agcgatcgtt ggcgccgcag gcctcaagcc gacgctcgcc
    13621 gcagtgcgcc aaggcacctg caccgcgctc gccaacaagg agtgtctcgt ctcggccgga
    13681 ccaatcttca tgcgcgaggt cgccaagtat cggacggcgc tgctgcccgt cgactcggag
    13741 cattcagcgg caatgcaggt catggccggc ggcgcgccgg agagcatcga gcgcatctgc
    13801 ctcacggcct ccggcgggcc gttccgctcg tggagcctcg ccgacatggc gagcgtaacc
    13861 ccgaagcagg cgctcgccca tcccaactgg tcgatgggcc cgaaggtcac catcgattcg
    13921 gcgacgctga tgaacaaggc gctcgagctg atcgaagcgc atcatctgtt ctcggtgccg
    13981 gcagagcggc tttctgttct gatccacccg caatccatcg tgcactgtct cgttcattat
    14041 cgcgacggct cggtgctggc gcagatgagc tgccccgaca tgcgcacgcc catcgcttac
    14101 agccttgctt ggcccaaccg catgcaagcg ccgaccgcgc ggctcgatct tgccagtctc
    14161 ggcgcactgt ccttcgaggc gcctgacgag gagcgcttcc cggccttgcg gcttgcacgc
    14221 caggtgctcg ccgccggagg cggctcgagc acggtgctga acgccgccaa cgaggtcgcg
    14281 gtacaagcat tcctcgacgg ccgcatcggc ttcttgggaa tcgcggccct ggtcgaggcc
    14341 acgctcggcc gctccgccca catttccgcg ccttcgcccg atagcgtcga tgaggtcctc
    14401 gccgtcgatg ccgaagcgcg ggccatcgcg ctctcgcaac tcctgcgatt tgcacaatcg
    14461 gctcaggcgc gctaggaagc cttacgcatc caggataccc tatggaaacg atactcaagc
    14521 tttcaggctt cggcgacacc gtgctgtctt atctgctccc cttcctcttc gtgctcacca
    14581 tcgtggtatt cttccacgag ctcggccatt ttcttatggc ccgctggtgc ggcgtggcgg
    14641 tcaagacatt ctcgatcggt ttcgggcctg agatcgtcgg cttcaacgac aggcacggga
    14701 cgcgctggcg gctctcctgg atcccgctcg gcggctatgt gaaattcatc gacgacgaga
    14761 atgcagcgag cgcagggcag aggcctctcg aagcgctgaa cgaagaggag cgcaagacca
    14821 gttttcaggg caagtcgctt ggcgcccgcg ccgccatcgt cgccgccggg ccgatcgcca
    14881 atttcgtact cgccatcgtc atcttcacgg cgatctttac cttcttcggc gagcggagca
    14941 ccgcggccaa ggtcgacatc gtcaatcccg gcagctccgc cgagcgcgcg ggcttcaaga
    15001 ccggcgacct cgtggtcaag atcgacggca cgacgatcga gaacttcggc cagatgcagc
    15061 gcatcgtcgg catgagcccc aaccagcagc ttcatttcga ggtcgagcgt gggggcaagc
    15121 ctgtcgagct gaccgccacg cccgagctga aagagatcac cgacaatttc ggcaacacca
    15181 tgcgcatcgg cctgttgggc attcagcgca gcgcgtcacc cgatgactgg accttgaagc
    15241 ggcacgatcc gctgaccgcc ttcgtcatgg cggtcaagga gtgctacttc gtgatctccc
    15301 gctcgctcgg ctatctctat gacgtgatca cgggccgcga agccgccgat cagcttggcg
    15361 gccccatccg aatcgcgcaa gtgtcgggac aagttgccac ggcaggattc tttgccctgc
    15421 tcaatctcgc cgccatcatt tcggtcagta tcgggctgat caatctgttc ccgattccca
    15481 tgctcgatgg cggtcacttg ctgttttacg gaatcgaggc ggtccgcggc aaacctttga
    15541 gcgaaaacat acaggaaatc ggtttccgca ttggccttgc cttcgtcctt atgctaatga
    15601 tcttcgcgac ctggaacgac ttgattcact tgaagtttct ttaagctgcg gcgtcaatca
    15661 atacacaacg actacgcgta gttttgctta cgccccccgt gggggttcgg accaaaatcg
    15721 gccacaaaaa ctggccactt tcgatcctgg gctctgcgtc caacggggga tcggtgttga
    15781 ggggacgaca ccagtgatgg tgcggtcgtg gttggctaga gggctgtctg cggccctgct
    15841 cgcagcaggc gcgatcgtgc tgtcgatcgg catgagcgct agcctgggcg agctttccgc
    15901 ctacgcccag gctgggcccg ccgttggcgc catccgtgtc gaaggcaata agcgcgtcga
    15961 gcccgaaacc gtccgcacct acctcacctt caatgtcggc gatccgtacg acctcgccaa
    16021 ggtcgacgaa tcgctaaagg ctctatttgc caccggcttg ttccaggatg ttcgcatccg
    16081 gcgcgaaggc agcaccgtgg tcatcgtggt ggtcgagaac ccgatcgtgt ccagggtcgc
    16141 ctttgaaggc aacaaggagg tcgaggacga cgcgctgacc gccgaggttc agctcaagcc
    16201 ccgcaccgtc tacacccgcg cccgcgtgca ggccgacgta cagcgcattc tcgatgtcta
    16261 tcgccgccag ggcctctatg cggctcaagt cgatcccaaa atcatcaagc tcgaaaacaa
    16321 ccgcatcgac gtcgtgttcg agatcaatga gggaccgacc accaaggtcc gcgccatcaa
    16381 cttcatcggc aacaccgcct tcagcgattc tcagcttcgc tacgtgatca gcaccacgca
    16441 gaccacgctg ctgagcttct tgaagagcac caacatctac gacccagacc ggctcaacct
    16501 cgatcgcgag ttgctccggc agttctatct caagaacggt tttgccgacg ttcgggtcat
    16561 ctcggccacc gccgacctcg accgcgacgg gcggggcttt ttcatcacct tcaccctcga
    16621 cgaaggcccg aaataccgct tcggggacat cgacgtagag accactctcc cctcgctcga
    16681 cgtgcgcacg ctgcgccgct ccctcgtgac ccgaaccggc cgcgtctata acgtccaaag
    16741 aatcgagtcg acggccgagg ctctgaccat catctcctcg caacagggct atgccttcgc
    16801 tcaggtcaag cctcgcttcg accgcgatga agtcaaccgc cgcatcaaca tcacctatgt
    16861 catcgaggaa ggtccgcaca tctatatcga gcgcatcaac atcgtcggca atttccgcac
    16921 ccacgacgac gtgattcgcc gcgagttcag tctcgccgag ggcgacgcct tcaaccggct
    16981 gctggtcgag gctgcccggc ggcgcttacg ctcgctcggc ttcttcaaga ctgtcgatat
    17041 cgacaccgag ccaggtagcg ccccggaccg tgtcgtgctc aacgtcacgg tgcaggagca
    17101 gccgaccggc gagctgtcct tcggcgccgg ctactccacg agcgagggtg tgatcggcga
    17161 catcagcatc accgagcgca acctgatggg cagaggccag tatgtcaggc tgggcttctc
    17221 cggaagcctt cagcgcgccc aggtcgactt cagcttcacc gagccgcatt tcctcgaccg
    17281 caatctcgcg gccggcttcg atctcttcca caaggaggtc gacttcacca acgtggcgtc
    17341 ctaccgggag cgcgacaccg gcggcaatct caggatggga ttcccgatcg ccttcaatac
    17401 ccagcttggt cttcgctacc ggctcgagcg cgaagaaatc tacgacgtcg ccgacaacgc
    17461 ctcgctggcg gtgaaggaag ccgagggcga atccatcgtg tcgagcgtcg gcttcacggt
    17521 tgcctacgat acccgcaatc tgccgcaatc tccgaccagc ggtatcttct cgtccttctc
    17581 cg
//
LOCUS       DATNAE010000198         2270 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_4403126, whole genome shotgun sequence.
ACCESSION   DATNAE010000198 DATNAE010000000
VERSION     DATNAE010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4403126"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1212)
                     /locus_tag="VN518_04570"
     CDS             complement(<1..1212)
                     /locus_tag="VN518_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HXE03880.1"
                     /translation="MSQLDKSTFLYGPNGTFIAELYSRYLEDPNAVDDSWRQFFADLA
                     EEAPGLLKELEGPAWSRERSRVIGNGHTAIAGNGHATPAVAAVAPAGVDPQQATLDAL
                     RALTLIRAYRVRGHLIADLDPLGLAGLSSHPDLDPATYGWGEGDYDRPIFVNGVLGFR
                     TATLREILAALKATYCGHVGIEYMHMQELEQRQWIQARVEVPRNQTEFTKTGKRAILQ
                     RLTEAELLERFLDRRYTGTKRFGLEGSEALIPALEQVIKRGGQLGMKELILGMPHRGR
                     LNVLTNMMGKPFAALFSEFQGNPANPADVQGSGDVKYHLGTSTDRDFDGNTVHLSLTA
                     NPSHLEAVDPVVLGKVRAKQRQRGDTSRDAVVGLLMHGDAAFAGQGLVAESLELSELK
                     GYRSGGTIHFIV"
     gene            complement(1383..>2270)
                     /gene="mdh"
                     /locus_tag="VN518_04575"
     CDS             complement(1383..>2270)
                     /gene="mdh"
                     /locus_tag="VN518_04575"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HXE03881.1"
                     /translation="LGDVVLFDIMEGVPQGKALDLAEASPVEGFDAKIAGGNDYSIIA
                     GADVVIVTAGFPRKPGMSRDDLIGKNAEVVATVGQNIKKHCPNAFVITITNPLDVMVW
                     VMREASGLPHQRVIGMAGVLDSARFRYFLADDMGVSVEDVSAFVLGGHGDDMVPLIRY
                     STVAGIPLPDLVKMGWTTQERVDKIVQRTRGGGGEIVNLLKTGSAFYAPAASAIAMAE
                     SYLRDKKRVLPCAAWLTGQYGVKDLYVGVPVVIGAKGVERIVEISLNPEEKAMFEKSV
                     NAVRGLVEATKKIQAGAKA"
BASE COUNT          373 a          722 c          768 g          407 t
ORIGIN      
        1 gacgatgaag tggatggtgc cgccgctgcg atagcctttg agctcggaaa gctcgagcga
       61 ttcggcgacg agcccctgcc cggcgaacgc ggcatcgcca tgcatgagca agcccacgac
      121 ggcgtcgcgc gacgtgtcgc cgcgctgccg ttgcttcgcc cgcactttgc ccagcacgac
      181 cggatcgacc gcttcgagat gcgagggatt ggcggtgagc gagagatgca ccgtgttgcc
      241 gtcgaaatcg cggtcggtcg aggtgcccaa atggtatttg acgtcgcccg agccttggac
      301 atcggccggg ttcgcgggat tgccctggaa ttccgagaac agcgccgcga agggcttgcc
      361 catcatgttg gtcagcacgt tgaggcggcc gcgatgcggc atgccgagga tcagctcctt
      421 catgccgagc tgaccgccgc gcttgatcac ctgctccaac gccgggatga gcgcttcgct
      481 gccttccaat ccgaagcgct tggtgccggt gtagcgccgg tcgaggaagc gttcgagcag
      541 ctcagcctcg gtcaggcgct gcaggatcgc gcgcttgccg gtcttggtga attcggtctg
      601 gttgcgcggg acttcgacgc gcgcctggat ccattggcgc tgctcgagct cctgcatgtg
      661 catgtattca atgccgacat gcccgcaata ggtggccttg agcgccgcga ggatctcgcg
      721 cagcgtcgcc gtgcgaaagc cgagcacgcc gttgacgaag atcggacggt cgtagtcgcc
      781 ctcgccccag ccataagtgg cgggatcaag gtccgggtgc gaactcagcc cggcgagacc
      841 cagcgggtcg agatcggcga tcagatgccc gcgcacgcga taggcccgaa tgagggtgag
      901 ggcgcgcagc gcgtcgagcg tcgcctgctg cggatcgacc cctgcgggag cgacggcggc
      961 gacggccggc gtggcgtggc cgttgccggc gatggcggta tggccattgc cgatgacgcg
     1021 gctgcgctcg cggctccagg cgggcccttc gagctccttg agcaagcccg gcgcctcctc
     1081 ggcgaggtcg gcaaagaatt gccgccagct atcgtcgacc gcgttggggt cttcgagata
     1141 gcgcgaatag agctcggcga tgaaagtgcc gttcgggccg tagaggaagg tggatttatc
     1201 caattgcgac atgggctttc cgaccgcatc tggcggggat ggggaggctg gacagccccc
     1261 ctgaaggctt tgggctaggc ttagagatgg gatgtggcgg cccgcttcct accccagcaa
     1321 aggttcatgc ctggtttacg cctgcctccc ggcttcctct ttctcctcgc cgcgcccttg
     1381 gcctaggctt tcgcccccgc ctggatcttc ttggtcgctt cgacgagacc gcgcaccgcg
     1441 ttcacggatt tctcgaacat cgccttttcc tcgggattga gcgagatctc gacaatgcgc
     1501 tcgacgccct tggcgccgat caccacggga acgccgacat agaggtcctt gaccccgtat
     1561 tggccggtca gccaggcggc gcagggcaac acgcgcttct tgtcgcggag ataggattcc
     1621 gccatggcga tggccgaggc ggccggggcg tagaaggccg agccggtctt gagcaggttg
     1681 acgatctcgc cgccgccgcc gcgggtgcgc tgcacgatct tgtcgacgcg ctcctgcgtc
     1741 gtccaaccca tcttgacgag atcgggcagc ggaatgccgg cgaccgtcga atagcggatg
     1801 agcggcacca tgtcgtcgcc atgaccgccc agcacgaagg cgctcacatc ctcgaccgag
     1861 acgcccatgt cgtcggcgag gaaatagcgg aagcgggcgc tgtcgagcac gccggccatg
     1921 ccgatgacgc gctgatgcgg caatcccgag gcctcccgca tcacccacac catgacgtcc
     1981 aggggattgg tgatggtgat gacgaaagcg ttggggcaat gcttcttgat gttctgcccg
     2041 accgtggcga cgacttcggc attcttgccg atgaggtcgt cgcggctcat gcccggcttg
     2101 cgcggaaagc ccgccgtgac gatcacgaca tcggcgccgg cgatgatgga atagtcgttg
     2161 ccgccggcga tcttggcgtc gaaaccctcg accggcgacg cctcggcgag gtcgagcgcc
     2221 ttgccctgcg gaacgccctc catgatgtcg aagagcacga cgtcgcccag
//
LOCUS       DATNAE010000199         4891 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_190977, whole genome shotgun sequence.
ACCESSION   DATNAE010000199 DATNAE010000000
VERSION     DATNAE010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4891
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190977"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VN518_04580"
     CDS             <1..460
                     /locus_tag="VN518_04580"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HXE03882.1"
                     /translation="PNPHVVPITKEQLEGVLDPMTAAFLFARSGNPNGDPKVCDQTIP
                     VFDGEARFNLILEPKRTVKLQKDASTGYSGFAAVCRVKFIPISGYRPDNPGIKLMSET
                     NEIEVWLVSLPGTEMYVPYRIVLPTLVGYGSATSTSFNVQRGAKRASLQD"
     gene            844..3615
                     /locus_tag="VN518_04585"
     CDS             844..3615
                     /locus_tag="VN518_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HXE03883.1"
                     /translation="MRGRGVTAVLGPTNTGKTHLAIERLLAHDVGIIGLPLRLLAREV
                     YDKIVARIGPAKVALITGEEKIKPPSPRHYVCTVEAMPLDLEADFVAVDEIQLAADGE
                     RGHVFTDRLFHARGFSETLLLGAATMRDAIRELLPGANIVSRPRLSTLTYAGDKKITR
                     LPRRAAIVAFSAQDVYAIAELVRRQRGGAAVVLGALSPRTRNSQVALYQNGDVDFLVA
                     TDAIGMGLNLDLDHVAFASLRKFDGQAHRDLTPAELGQIAGRAGRHMNDGTFGVTGQV
                     PAFESELIDRLENHAFDQVGMLQWRNRKLDFGSIESLKESLRETPDHPRLIRSRMVDD
                     VIALENVSHDAGVRELASGRAGVALLWEMCQIPDYRKISAASHAELVGTLYRFVMSDT
                     GRIPADWFARQVALADRTDGDLDTLSNRLAQIRTWTFVSHRAPWLDDPEHWQGRTRAI
                     EDMLSDALHERLTHRFVDKRTSVLMRRLRDKEELVAEIGADGRVIVEDHFVGSLDGFR
                     FTPDTSSDGIHGRAARHAAVKVLAHELGARAAALSAASDADIVLKPNGRIAWRSSEIA
                     RLERGDGPLKPKIQLLTDEHLGAIEREQVLRRIEAWLSDQLKAKLAPLVALSEASDLS
                     GLARGLAFRVVETLGVLRRDAVAGEVKALDQIARAGLRKYGVRFGAFNIYIPALLKPA
                     AADLLLLLWALHAGRNYGIDAEALPPRPQQGLTSVEADPRVPEPYWHAAGFQIAGGRA
                     IRIDMLERLSDLIRARVSWRPTEDGGPAPSGAIGDGGFRAAPELMSVVGCSGEDFASI
                     LKALGFRRERQTLEPVDERAIKPAPDGNGSEGSEVPAPANETATQPDIAFDEIWRPGK
                     RNEARKPETHHAKGKPRRGARQAQAPRQREQRPGLERKERPRPSDSSPFAVLAELRRN
                     LAARRPEGN"
     gene            3625..3999
                     /locus_tag="VN518_04590"
     CDS             3625..3999
                     /locus_tag="VN518_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HXE03884.1"
                     /translation="MEGQRLDKWLWCARLVKTRSLAAKLIEGGKVRVNGERAPKVSRH
                     VRMGDVVTGTGVGAGRLFVVRVIGEAERRGPATIARSLYEDLTPEAPRSASGDALIEV
                     HHGKRPTKRDRRRLEAIKSGNV"
     gene            4175..4513
                     /gene="fdxA"
                     /locus_tag="VN518_04595"
     CDS             4175..4513
                     /gene="fdxA"
                     /locus_tag="VN518_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HXE03885.1"
                     /translation="MTYVVTEVCIKCKYTDCVEVCPVDCFYEGENMLVIHPDECIDCG
                     VCEPECPVDAIKPDTEPSLDRWLDLNREYSEKWPNITTKKAAPPDADAFVSEPEKFTK
                     YFSTNPGEGD"
BASE COUNT          896 a         1616 c         1507 g          872 t
ORIGIN      
        1 gccaaatccg cacgtcgtcc ccatcaccaa ggagcagctc gaaggcgtgc tcgacccgat
       61 gacagcggcg tttctctttg cgcgctccgg caaccccaac ggcgacccga aggtctgcga
      121 tcagaccatt ccggtgttcg acggcgaggc gcgtttcaac ctcatcctcg agcctaagcg
      181 cacggtgaaa ctgcagaagg atgcatcgac cggatattcg ggcttcgccg cggtctgccg
      241 tgtgaagttc attccgatct ccggctaccg acccgacaat cccggcatca agctcatgtc
      301 ggagacgaac gagatcgaag tctggctcgt ctcgctgccg ggcaccgaga tgtacgtccc
      361 ctaccgcatc gtgctgccga cccttgtggg ctacggctcg gccacctcga cctcattcaa
      421 cgtccagaga ggcgcaaagc gcgccagcct ccaggattaa ccccctcatt tgccggcctg
      481 ccaagggtta acggcaaggc atctaatccc aatatgtagt agcgaacagc tggcgaatat
      541 taacggtctc ctgtttcgtc cgtgttccgt tccctttgtt cccctggggc gctgagcctc
      601 acgtgaagcc tccgcgtcaa ctggttgatt cacggtcgcg aacacgcatt gcgcgatgag
      661 ctttgataga gcgagctcga tctggtgacg ccggtggaga acgaaatccg aatggcccga
      721 gtcagcgatt cggacgagat tcgttccccc ctcgttccag acctagcgcg tttgtcccaa
      781 tacgctatca gtaggtcctt agggtctaac agcatgagcc gacttccctc aagttccgga
      841 gcaatgcgcg gccgaggcgt caccgcggtg cttgggccga ccaacaccgg caagacccac
      901 ctcgccatcg agcgcctgct cgcccacgac gtcggcatca tcggtctgcc gctccgtctg
      961 ctcgcccgcg aggtctacga caagatcgtc gcgcgcatcg gccccgccaa agtcgcgctg
     1021 atcaccggcg aagagaagat caagccgccc tcgccgcgcc attatgtgtg caccgtcgag
     1081 gcgatgccgc tcgatctcga ggccgacttc gtcgccgtcg acgagattca gctcgccgcc
     1141 gacggcgagc gcggccacgt cttcaccgat cgcctgtttc acgctcgcgg cttcagcgag
     1201 acgctgctgc tcggcgccgc caccatgcgc gacgccatcc gcgaattgct tcccggcgcc
     1261 aatatcgtct cgcggccgcg cctctccacg ctcacttacg ccggcgacaa gaagatcacg
     1321 cgcctacccc gccgcgccgc catcgtcgcc ttctccgcac aagacgtcta cgccatcgcc
     1381 gagctggtgc ggcggcagcg cggcggcgcc gccgtggtgc tcggcgcgct ctccccgcgc
     1441 acgcgcaatt cccaagtcgc cctctaccag aacggcgacg tcgatttcct ggtggcgacc
     1501 gacgccatcg gcatgggcct caatctcgat ctcgaccatg tggccttcgc gtcattacgc
     1561 aaattcgacg ggcaagcgca ccgcgatttg acaccggccg agctcggcca gatcgccggc
     1621 cgcgccggcc gtcacatgaa tgacggcacc ttcggcgtta ccggccaggt ccctgccttt
     1681 gaatcagagc tgatcgatcg gctggagaac catgccttcg atcaggtcgg catgctgcaa
     1741 tggcgcaatc gcaagctcga tttcggctcg atcgaaagcc tcaaggaaag cttgcgcgag
     1801 acgccggacc atccgcggct catccgctcg cgcatggtcg acgatgtgat tgcgctcgag
     1861 aatgtcagcc atgacgccgg cgtgcgcgag ctcgcttccg gccgtgccgg cgtggcgctt
     1921 ctctgggaga tgtgccagat cccggactac cggaagatct cggcggcaag ccacgccgag
     1981 ctcgtcggca cgctctatcg cttcgtgatg agcgacactg gccggattcc cgccgattgg
     2041 tttgcccgcc aggtggccct cgccgaccgc accgacggcg acctcgacac actgtccaat
     2101 cggctcgcgc agattcgcac ctggaccttc gtctctcacc gcgcaccgtg gctcgacgat
     2161 cccgagcatt ggcagggccg caccagggcg atcgaggaca tgctgtccga cgcgctgcat
     2221 gagcggctga cgcatcgctt cgtcgataag cggacgagcg tgctgatgcg gcgcttgcgc
     2281 gacaaggaag agctcgtggc cgagatcggc gccgacgggc gtgtcattgt cgaggatcat
     2341 tttgtcggca gcctcgacgg attccgcttc acgcctgaca cgagcagcga cggcattcac
     2401 ggacgcgccg cccgccacgc cgccgtcaaa gtgctggcgc atgagctcgg cgcccgcgcc
     2461 gccgccctct cagcggcgag cgacgccgac atcgtgctca agccgaacgg ccgcatcgcc
     2521 tggcgctcat cggagatcgc acggctcgag cgcggcgacg ggccgctcaa gccgaagatc
     2581 cagcttttga ccgatgagca tctcggtgcc atcgagcgcg agcaggtctt gcgccgcatc
     2641 gaggcttggc tctccgacca gctcaaagcc aagctcgcgc cgctcgttgc gctgagcgaa
     2701 gcttcggatc tctcagggct cgcccgcggc ctcgccttcc gcgtggtcga gacgctcggc
     2761 gtgctgcgac gcgacgcggt cgccggcgaa gtcaaggcgc tcgatcaaat cgcccgcgcc
     2821 ggcttgcgca aatacggcgt gcgcttcggt gccttcaaca tctacatccc ggcgctgctc
     2881 aagcccgccg ccgccgatct cttgttgctg ctgtgggcgc tccatgccgg ccggaactac
     2941 ggcatcgacg ccgaggctct gccgcccagg cctcagcaag ggctcacctc ggtcgaggcc
     3001 gatccgcgcg tcccggagcc ctattggcac gcggcaggct tccagattgc cggcggccgc
     3061 gcgatccgca tcgacatgct ggagcggctg tcggacctca tccgcgcccg cgtgtcgtgg
     3121 aggccgaccg aggacggcgg gcccgcgcct tccggcgcca tcggcgacgg cggctttcgc
     3181 gcggcgcccg agctgatgag cgtggtcggc tgctcaggcg aggatttcgc ctcgatcctg
     3241 aaggcgctcg gtttccggcg cgaacggcag acgctcgagc cggtggatga gcgcgccatc
     3301 aagccggcgc ctgacggcaa tggctctgaa ggctctgaag tgccggctcc ggccaacgag
     3361 accgcaactc agccagacat cgccttcgac gagatctggc gtccaggcaa gcggaacgag
     3421 gcgcggaaac ctgaaaccca ccatgcaaaa ggcaaaccgc gccggggcgc ccggcaagca
     3481 caagccccgc gccagcgtga gcagcggccg ggacttgagc gcaaggagcg cccgcgcccg
     3541 tccgactctt cgccttttgc cgtgctcgcc gagcttcgcc gcaatcttgc cgcgaggcgc
     3601 cctgagggta actgacgcga tcaaatggaa ggtcagcggc tcgacaaatg gctgtggtgc
     3661 gcgcggctcg ttaagacgcg atcgcttgcc gccaagctga tcgagggcgg caaggtcagg
     3721 gtcaatggcg agcgtgcgcc gaaagtgagc cgccatgtgc gtatgggcga cgtggtgacg
     3781 ggaaccggcg tgggcgccgg tcgcctcttc gtggtgcggg tcatagggga ggccgagcgg
     3841 cgcggaccgg cgacgattgc ccgctcgctc tacgaggatt tgacgcctga ggcgcctcgt
     3901 tcggcttcag gggacgcgct gatcgaggtc catcacggca agcggccaac caagcgcgac
     3961 cgcaggcgcc tcgaggcaat caagtccggc aatgtttgag gagcggagga tctaaggccc
     4021 acgctttgag cccaatcgat agcaattgct tgccttgtac tggtgccgcg attgggatag
     4081 gcaaagcccc tcatcggccc caagttaatg ggagcgacgc ggccggaatg aagggctgaa
     4141 gcttgttgct ttggttctaa gcgaagtggg atcgatgacg tacgtcgtca ctgaagtctg
     4201 catcaagtgc aaatacaccg actgcgttga agtctgcccg gtggactgct tctacgaggg
     4261 cgagaatatg ctcgtgatcc atcccgatga atgcatcgat tgcggggtgt gcgagcctga
     4321 atgcccggtc gacgccatca agcccgacac cgagccttcg cttgaccgtt ggctcgatct
     4381 caatcgcgaa tattcggaaa agtggccgaa catcacgacc aagaaggcag caccccctga
     4441 cgccgacgcc tttgtcagcg agccagaaaa gttcaccaaa tatttcagca ccaacccagg
     4501 cgaaggcgat taggggcgcg ggctctcaaa ttattcacct tactgtcaaa atgtcgcatg
     4561 ttgcggtgca aaacggggcg tgctgcggca ccgcatgact ttcggattta ctaagattgt
     4621 gttatattgg tgttgaaatg gcgcggattg ccgccagtga gcaccaagag aggcgtccat
     4681 ttttgggcgc actttttttt ccgacttgag ggaggtggca cgatcaggcc tctgatcgat
     4741 ggctcatcgt gtgcttgttg tcatccgtca aatcatgacg ggcgtcgaaa attgtgactg
     4801 gcgcatggcg ataggcgagc ccgcctccgc cgcaccgcgc tcgcaaagaa ttctgacaaa
     4861 gtgagatgag agtaatgcct gacaagaaaa a
//
LOCUS       DATNAE010000200         3026 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3495372, whole genome shotgun sequence.
ACCESSION   DATNAE010000200 DATNAE010000000
VERSION     DATNAE010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3495372"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..1933)
                     /locus_tag="VN518_04600"
     CDS             complement(107..1933)
                     /locus_tag="VN518_04600"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:NF005089.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HXE03886.1"
                     /translation="MAQWIRFEKGGRVGFGTLEDGTISVHSGDMFAGAKATGEKVKLA
                     EVRVLTPCDPSKMICLWNNFHQLAAKNDFLVPDEPLYFLKGPNAYHPHGAPIERPKSY
                     SGRIVYEGELGVVIGKKCSMVSEAEAGDYIFGYTCVNDVTAVDLLRKNPSFEQWARCK
                     SFDTFGVFGPVITTGVDPMKLVIRAILNGKERQNYPVSDMFFPPHKLVAMVSRDMTLM
                     PGDIISCGTSLGAGPMPDAQNVIDVAIDGVGVLSNRFDQVLPSPYLLKDAPAPIKACV
                     VGAGAIGGLVAARLALAGNEVTVIDMGAHLAAIKAKGLTLEWHDGKVETAKVKGVDKA
                     ADAGKQDLVVLAVKAHYLDQVVKDIDHLLGPDTIVMTVQNGLPWWYFQRLGGKYDNKK
                     LETLDPTGILTRKIDASRLIGCVVYPAAAVTAPGVIHHVEGDRFPIGELDGKDTERVK
                     RLHDVLVKAGLKSRVLKDIRSEIWLKAWGNLSFNPISALTHATLVAICQFPETRHLAA
                     RMMEEAQTIAQKLGVTFRVSIEKRIAGAEAVGAHKTSMLQDVEAGRSLETEALIGSIL
                     EMAKLTETPAPAIESVYALVKLLNKVMLLEGGGVRVDKAAAE"
     gene            2171..>3026
                     /locus_tag="VN518_04605"
     CDS             2171..>3026
                     /locus_tag="VN518_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXE03887.1"
                     /translation="MRIAQVAPLAEAVPPRLYGGTERVVSYLTEELVELGHDVTLFAS
                     GDSLTQAELVPMVPRALRLEAMVRDPVALHIALVEAVSRRADEFDAIHVHFDHLHLPL
                     FTWRRLPFLTTLHGRLDMPELVPVMRAYPEAPFVSISDAQRRPLPFVDWLATVHHGLP
                     ASFPGRRPEPGGYLAFLGRICPEKGPDRAIRIAARAGMPLKIAAKVDAVDRAYHEAVV
                     EPLLREHAGLVEYVGEIAEHEKGAFLGAATAMLFPVDWPEPFGLAMIEAMACGTPVVA
                     YRRGSVPEV"
BASE COUNT          476 a         1007 c          981 g          562 t
ORIGIN      
        1 ctattgcgag gaaccaaagg cgacgacgca atctcccgcc tatgattccc ttcgccggcg
       61 ggagattgct tcgctccgct cgcaatgacg cggcagggag tagcgcttac tccgccgccg
      121 ctttgtcgac gcgcacgcca ccgccttcga gcagcatcac cttgttcaag agcttgacga
      181 gggcgtagac gctctcgatc gcgggtgctg gcgtctcggt aagcttcgcc atctcgagga
      241 tcgagccgat cagcgcctcg gtctcgagcg agcggccggc ctcgacatcc tgcagcatcg
      301 aggtcttgtg cgcgccgacc gcctcggcgc cggcgatgcg cttctcgatg ctgacgcgga
      361 aggtgacgcc gagcttctgc gcgatggtct gcgcctcctc catcatgcgc gccgccaaat
      421 gccgcgtctc ggggaattgg cagatcgcga ccagcgtcgc gtgggtgagc gcgctgatcg
      481 ggttgaagga gagattgccc cacgccttga gccagatctc cgagcggatg tccttgagca
      541 cgcgcgattt caagcccgcc ttcaccagca cgtcgtggag gcgcttgacg cgctcggtgt
      601 ccttgccgtc gagctcgccg atcgggaagc ggtcgccctc gacgtgatgg atgacgcccg
      661 gcgccgtcac cgccgcggcg ggatagacga cgcagccgat gaggcggctg gcgtcgatct
      721 tgcgcgtcaa gatgccggtg ggatcgaggg tctcgagctt cttgttgtcg tatttgccgc
      781 cgagccgctg gaaataccac caaggcagcc cgttctgcac ggtcatgaca atggtatcgg
      841 gaccgaggag atgatcgatg tccttgacca cctggtcgag gtaatgcgcc ttgacggcga
      901 gcacgacaag atcctgcttg ccggcatcgg ccgccttgtc gacgcccttg accttggcgg
      961 tctcgacctt gccgtcatgc cattcgagcg tcaggccctt ggccttgatg gcggcgagat
     1021 gcgcgcccat gtcgatgacc gtcacctcgt tgccggcgag cgcaagcctt gccgcaacga
     1081 ggccgccgat agcgcccgcg cccacgacgc aggccttgat cggcgccggc gcgtctttca
     1141 ggagataggg gctcggcagc acctggtcga agcggttgct gagcacgccg acgccgtcga
     1201 tcgcgacatc gatgacattc tgcgcatctg gcatcggccc cgcgcccaat gatgtgccgc
     1261 aggagatgat gtcgcccggc atcagcgtca tgtcgcgcga caccatggcg acgagcttgt
     1321 gcggcgggaa gaacatgtcg ctgaccgggt agttctgccg ctccttgccg ttgaggatcg
     1381 cgcggatgac gagcttcatc ggatcgacgc cggtggtgat caccgggccg aacacgccga
     1441 aggtgtcgaa gctcttgcag cgcgcccatt gctcgaagct tgggttcttc ctgaggagat
     1501 cgacggcggt cacgtcgttc acgcaggtgt agccgaagat gtagtcgcca gcctcggcct
     1561 cgctcaccat cgagcatttc ttgccgatga cgacaccgag ctcgccctca tagacgatgc
     1621 ggccggaata ggatttgggc cgctcgatcg gcgcgccatg cggatgatag gcgttgggac
     1681 ccttcaagaa ataaagcggc tcgtcgggaa cgaggaagtc gttcttggcg gcgagctgat
     1741 ggaaattgtt ccagagacag atcatcttcg aggggtcgca gggtgtcagc accctgacct
     1801 cggcgagctt cactttctcg cccgtcgctt tggcaccggc gaacatgtcg ccggaatgga
     1861 cggagatcgt gccgtcctcg agtgttccga atccgacgcg gccgcccttc tcgaacctga
     1921 tccactgcgc catccattcc tccctgtctt agccgaccgc ccgaccggcg atcccgcagc
     1981 ttgccatggc cagacggtgc cgtaccgcgc gccgtcgggc aagtctcaac cggcgatagc
     2041 gtcagcccgc agccgcgctg cgtccgaccc tattttagcc cacggcttga aacggacaca
     2101 ggatcgttcg cgctcgaccg ccgttagccg ccgtcatgcc cgccattccg gcggacggaa
     2161 aggaagagtc gtgaggatag cgcaagtagc cccgctcgcc gaggcggtgc caccgaggct
     2221 ctatggcggg accgagcgcg tcgtctcgta tctgaccgag gagctggtcg agctcgggca
     2281 tgacgtgacg ctcttcgcca gcggcgactc cctgacccag gcagagctcg tgccgatggt
     2341 gccgcgcgcc ttgcgcctcg aggcgatggt gcgtgacccg gtggcgctgc acatcgcgct
     2401 cgtcgaggcg gtgtcgcgcc gggccgacga gttcgacgcc atccatgtgc atttcgacca
     2461 tctgcacctg ccgctgttca cctggcggcg cctgccgttc ctgacgaccc tgcatggccg
     2521 cctcgacatg ccggagctcg ttccggtgat gcgggcgtat cccgaggcac ccttcgtctc
     2581 gatctccgac gcgcaacggc ggccgctgcc ctttgtcgac tggcttgcga ccgtccatca
     2641 cggcctgccc gcgagctttc ccgggcggcg ccctgagcct ggcggctatc tcgcctttct
     2701 cggccggatc tgtccggaaa aaggcccgga tcgcgcgatc cgcattgccg cccgtgccgg
     2761 catgcccctc aagatcgccg ccaaggtcga tgccgtcgac cgcgcctatc acgaggccgt
     2821 ggtcgagccg ctgctccgcg agcatgccgg gcttgtcgag tatgtcggcg agatcgccga
     2881 gcacgagaaa ggcgcgtttc tcggcgccgc cacggccatg ctctttcccg tcgactggcc
     2941 ggagccgttc ggcctggcga tgatcgaggc gatggcctgc ggcacgccag ttgtggccta
     3001 ccgtcgcggc tcggtgccgg aagtgg
//
LOCUS       DATNAE010000201         2088 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_4098596, whole genome shotgun sequence.
ACCESSION   DATNAE010000201 DATNAE010000000
VERSION     DATNAE010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4098596"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..946)
                     /locus_tag="VN518_04610"
     CDS             complement(<1..946)
                     /locus_tag="VN518_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003609314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HXE03888.1"
                     /translation="MTRIPAFADIAFDAGNGASTRPSGAAPWQTPEGIAVKPVYGPED
                     IARLDFLDGMPGVAPYLRGPYPTMYVQRPWTIRQYSGFSTAEDSNAFYRRNLAAGQQG
                     VSIAFDLATHRGYDSDHPRVTGDVGMAGVAIDSIYDMRTLFDHIPLDRVSVSMTMNGA
                     VLPVLALFIVAGEEQGVPPEELSGTIQNDILKEFMVRNTYIYPPAPSMRIVSDIFAYT
                     SKKMPKFNSISISGYHMQEDGATADLELAYTIADGIEYARAGVAAGLDIDRFAPRLSF
                     FWAIGMNFFMEVAKLRAARALWCEVMEELGAEDERSKML"
     gene            complement(943..>2088)
                     /locus_tag="VN518_04615"
     CDS             complement(943..>2088)
                     /locus_tag="VN518_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HXE03889.1"
                     /translation="VAYLRALEQGGMALDDARRVIYFRLAADQDQFLTIAKFRAIRKL
                     WARVEEACGLEPSRACVTAETAWRMMTRRDPHGNIVRGTIAALAAAVGGADAVTVLPF
                     SAALGIPDPFARRIARNTQTILIEEANLHRVADPAAGSGAIEYLTEQLCEKAWGLFQE
                     IERAGGSFAALKAGLIQKEVAKVRAEREANIASRKDTVIGTSDFPDLAEEPVAVLGAA
                     KAPPTRMPPGEPITPLARFRLAEPFERLRDLSDAYFARTGGRPKVFLACLGRASDFTA
                     RASFARSLFEAGGIEAVEGSGDTLMKRFKESGAKLACLCSSDKVYAGEAVSSAKALAT
                     AGATHIYLSGKPGDHEKAWREAGIGTLLYQGCDTLGLLQDAYERLGA"
BASE COUNT          374 a          639 c          702 g          373 t
ORIGIN      
        1 gcagcatctt gctgcgctcg tcctcagccc cgagttcttc catcacctcg caccacagcg
       61 cgcgggctgc gcgcagcttg gcgacttcca tgaagaagtt catgccgatc gcccagaaga
      121 acgacaggcg tggcgcgaag cggtcgatat cgaggccggc tgccacgccg gcacgggcat
      181 attcgatgcc gtcggcgatc gtataggcga gctccaggtc cgccgtcgca ccgtcctcct
      241 gcatgtgata gccggaaatc gagatcgaat tgaatttcgg catcttcttc gaggtgtagg
      301 cgaagatgtc ggagacgatg cgcatcgatg gggcgggcgg atagatgtag gtgttccgca
      361 ccatgaactc tttcagaatg tcgttctgaa tggtgccgga cagctcctct ggcggcacgc
      421 cttgctcttc gcccgcgacg atgaagagcg cgagaaccgg gagcaccgcg ccgttcatgg
      481 tcatcgaaac tgagaccctg tcgagcggga tgtggtcgaa caaagtgcgc atgtcgtaaa
      541 tagagtcgat ggcgacgcct gccatgccga cgtcaccggt gacgcgcgga tggtcggagt
      601 cgtagccgcg atgggtggca aggtcgaaag cgatggacac gccttgctgg cccgcggcga
      661 ggttgcggcg atagaaggcg ttcgagtcct ctgccgtcga gaagccggaa tattggcgga
      721 tggtccatgg ccgctgcaca tacatggtgg ggtaggggcc gcggagataa ggtgcgacgc
      781 ccggcatccc gtcgagaaag tcgaggcgcg cgatatcctc aggcccatag accggcttca
      841 ccgcgatgcc ttccggcgtc tgccaaggcg cggccccgct cggccttgtc gaggcgccgt
      901 tgccggcgtc gaaggcgata tcagcgaagg cggggattcg ggtcatgcgc ccagtctctc
      961 ataagcatcc tgtaataaac caagcgtatc acagccttga taaagcaatg ttccaatccc
     1021 ggcctctcgc caggccttct cgtgatctcc tggcttgcct gagagataaa tgtgggttgc
     1081 gccggccgtg gccagcgcct tggccgaact gacagcctcg cccgcgtaga ccttgtccga
     1141 agagcagagg caggcgagct tcgcgccaga ctctttgaat cgtttcatca gagtgtcgcc
     1201 gcttccttcc accgcctcga tgccgcctgc ctcgaacagg ctcctggcaa agctcgcgcg
     1261 ggcggtgaag tcggaggcgc gtccaagaca ggcaaggaac actttgggcc gtcccccggt
     1321 acgggcgaag taagcgtcgc tcaggtcgcg caagcgctcg aagggctcgg cgagccggaa
     1381 gcgagcgagc ggcgtgatgg gttcacccgg cggcatgcgg gttgggggtg ctttggccgc
     1441 gccgagcacc gccacgggct cttcggcaag atcggggaag tcgctcgtgc cgattacagt
     1501 gtccttgcgg ctcgcgatat tggcctcgcg ttccgcccga accttggcaa cttccttctg
     1561 gatcaacccg gctttcagcg ccgcgaagga accgccggcg cgctcgatct cctgaaacaa
     1621 gccccaagcc ttctcgcaga gctgctcggt gagatattcg atggcgcctg agcccgcggc
     1681 gggatcggcg acgcgatgca aattggcttc ctcgatgagg atggtctgcg tgttgcgggc
     1741 gatgcgccgc gcgaagggat cggggatgcc gagcgccgcg ctgaacggca gcaccgtgac
     1801 cgcatcggcg ccgccgacgg cagcggcaag ggctgcgatg gtgccgcgca cgatgtttcc
     1861 gtggggatcg cgcctggtca tcatgcgcca ggcggtctcc gcggtgacgc aggcgcgcga
     1921 gggctcaagc ccgcatgcct cttcgacgcg cgcccaaagt ttccggatcg cccgaaactt
     1981 ggcgatggtc aggaactgat cctggtcggc ggcgaggcgg aaatagatga cgcggcgcgc
     2041 atcgtcgagc gccatgccgc cttgctccag cgcgcggaga taggcgac
//
LOCUS       DATNAE010000202         7531 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3799218, whole genome shotgun sequence.
ACCESSION   DATNAE010000202 DATNAE010000000
VERSION     DATNAE010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 7531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7531
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3799218"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="VN518_04620"
     CDS             <1..227
                     /locus_tag="VN518_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006466059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HXE03890.1"
                     /translation="DADDQLIRAADRLKRIPGVIVHGRYDVVTPAKIAVDLARAWPEA
                     DLRIVADAGHAMTEAGIVHELVSATNRFAR"
     gene            complement(230..667)
                     /locus_tag="VN518_04625"
     CDS             complement(230..667)
                     /locus_tag="VN518_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HXE03891.1"
                     /translation="MAGLSQASHRLRVRTRGKGFIDITEDLRHWLGDISARDGLLTVF
                     VCHTSASLTIQENADPNVRRDLMTTLETLAPEDAPYTHAEEGPDDMPSHIKSMLTEVS
                     LGIPVMGGRMALGTWQGVYLIEHRDAPHERNIALSFIGKSAEA"
     gene            complement(709..1137)
                     /locus_tag="VN518_04630"
     CDS             complement(709..1137)
                     /locus_tag="VN518_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03892.1"
                     /translation="MTTRLLALAFVLFAVSPCYAGEDPGRYTMTTVGDSVWRLDSATG
                     AMSVCGQKLDHWACESVADDALALKAEADRLARENRELKDKLAKAEEREAEAGPLPAN
                     PSTQFPGLALGEMNDFINKMIRSLQDLVRDLKRQEAGQEL"
     gene            1359..3134
                     /locus_tag="VN518_04635"
     CDS             1359..3134
                     /locus_tag="VN518_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03893.1"
                     /translation="MAPEVRWQASSAAGSSGSETRAPDRESVESLLRKLIKRIEESER
                     RYEEALDELHARLGQVSYSASVNEALGTPEETETLERLRRQLSDLARRLEQPQPKKEA
                     ERLPRLDKALDEVRAVSTGLALAEPDWFASLSASLAAAEGRAEPEPVPAPLPASEPRY
                     TSRSFAPSPSNDEDADFDRRLIEMAQRLERSIGEALPSSSIEKLNARMEEISARFETA
                     LAQEPKLETLHHIERQVADMGFQLGRVEQQAARIGAVEGQLQRLVERIESTPAQIERT
                     ATKAAQETARLVSETGLGKPSAAERLDSLHRDIVAMNERNLATDDRLVDTLTAMHESL
                     KGLMQRERDRAAATAAREAPPRVEPAASEAGPADFAQSRQPVAAAQIQAAEPPRQRTT
                     FIRDIPFESTEDLVAAARRAAQAAAARAEERNALPQRKVRATAETKPSEEPGRHKVSL
                     LMVAAAILLMISAALLLTRLKLKPDFDGPPPAAEQSMPAPAAEAPPLPDAPLQTEVIP
                     SPEGESMPSGAAAPEVLPEPEAPDAAPTAPHAVTPSPSPDGTGARSPVHLGDGADEAL
                     VQPVSLSPSGGRSLPPGVSVMVVEP"
     gene            3304..4233
                     /locus_tag="VN518_04640"
     CDS             3304..4233
                     /locus_tag="VN518_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007200065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HXE03894.1"
                     /translation="MLQVYLPVAEISVNLLVMLGLGAAIGFLSGMFGVGGGFLLTPLL
                     MFSGIPPAIAVATGANQIVASSVSGGLVQWRRGNIDFRMGWVLIIGGVFGAVAGVLLV
                     GLLSRLGHADVFISLVYVTFLGVIGSLMLVESVSAIRRTRAGLPGRARKPGEHTWIHG
                     LPFKMRFPRSRLYISAIPPLILGAIVGLLASILGVGGGFIVVPGMIYLLRMPTNVVIG
                     TSLFQIIFVTAVVTILYAVLNHSLDILLALLLVIGGVIGGLFGADAGQRLKGEQLRLL
                     LAVMVLAVALRLLVGLLVPPEDLYSLGRVVTGA"
     gene            4237..5019
                     /locus_tag="VN518_04645"
     CDS             4237..5019
                     /locus_tag="VN518_04645"
                     /inference="COORDINATES: protein motif:HMM:NF021148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02186 family protein"
                     /protein_id="HXE03895.1"
                     /translation="MQPARLLALLAVALLPGRQTFAAEPPRAEQVQSDVSTREISIQS
                     NFTGVEIVLFGSIDFTRAPSLDEGPYDVIMVIRGPNRPIVVRRQERIAGLWMNGASET
                     FPSVPGYYAVLASRPFRAVASEAVLKNLGIGFSNLDFGKTAEGESEADGFRASLIRLQ
                     EEHDLFQESDDAISFIGRSLFRGSVQLPVNVPIGRYTTQVFLFRDGKLLSQSQNSLQV
                     EKVGVERVVYMLAFHYPFAYGLLAVLMAVSAGLLAWTVFRRE"
     gene            complement(5035..5745)
                     /gene="pdeM"
                     /locus_tag="VN518_04650"
     CDS             complement(5035..5745)
                     /gene="pdeM"
                     /locus_tag="VN518_04650"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     endonuclease PdeM"
                     /protein_id="HXE03896.1"
                     /translation="MLRLMSEVLAGRRPPIRLAGHSFLPVAAGALYWESEDTLLVADL
                     HLEKGAAFAARGMMLPPYDTRSTLQRLAACLDHFSPRRVVALGDSFHRSELAGRLSAD
                     DREELAALQQGRDWYWILGNHDPDLPESIGGFVCRSLMIGGITLCHQPSLSRAPEIAG
                     HLHPVARIARRGEGIRRKCFATDGHRLVMPAFGAYAGGLNVLDEAFSSLFHWHLMQAW
                     LTGRTSVYPLQASLLLPD"
     gene            complement(5726..>7531)
                     /locus_tag="VN518_04655"
     CDS             complement(5726..>7531)
                     /locus_tag="VN518_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007796321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HXE03897.1"
                     /translation="WAGHSARYALPEIYEAIKAHRMSLIFVNTRSQAEMLFHALWRYN
                     DANLPLALHHGSLDVGQRRKVEAAMVAGKLRAVVCTSTLDLGIDWGDVDLVINVGAPK
                     GASRLAQRIGRANHRLDEPSKALLVPANRFEVVECQAALEAAEEGAQDTPPSRPGALD
                     VLAQHVLGMACSGPLDPDQLYREVTSASPYQGLTREGFDKIVRFVSTGGYALANYERY
                     AKLKQMPDGRYRIAHPRLAQQYRLNVGTIVEAPMVRVRLARRQGGARRSSAVLAAGRV
                     LGEVEEYFIEQLMPGDSFVFAGEVLRFEGLREPDAIVTRSHAPEPKVPSYDGGKFPLS
                     TYLADRVRRLLADRRHWARLSDQVSDWLRIQSEVSVVPGPDEILIETFPRGQRHYLVC
                     YPFEGRLAHQSLGMLLTRRLERAHAKPLGFVANEYALCVWGLADTGEMVRTGRLDLAK
                     LFDEDMLGDDLDAWLAESSLMKRSFRVCAIISGLIERRHPGQEKTGRQMTVSSDLIYA
                     VLRQHEPDHILLQAAFADAATGLIDVGRLSGFLQRIRGRIRHIALDRVSPLAVPAMLE
                     IGRESMPGDASEALLREAVEDLIAEAMGDHAALDV"
BASE COUNT         1297 a         2499 c         2450 g         1285 t
ORIGIN      
        1 tcgatgccga cgaccagttg atcagggcgg cagaccgtct caagcggatc ccgggcgtga
       61 tcgtgcatgg ccgctacgac gtggtaacgc cggcgaagat cgccgtggac cttgcccgcg
      121 cctggccaga ggccgatctg cgcatcgtcg ccgacgcagg tcacgccatg accgaggccg
      181 gcatcgtgca tgagctggtc tcggccacca accgctttgc ccgctgagat taggcctcgg
      241 cggacttgcc gataaagctg agcgcgatgt tacgctcatg cggcgcgtca cggtgctcga
      301 tgagatagac gccctgccag gtgccgagag ccatccgccc gcccatcacc ggaattccga
      361 ggctgacctc ggtcagcatc gacttgatgt ggctcggcat gtcgtcgggc ccctcctcgg
      421 catgggtgta gggagcgtct tccggcgcga gggtctcgag cgtcgtcatc agatcgcgcc
      481 gcacgttggg atcggcgttc tcctgaatgg tgagcgacgc cgaggtgtga cagacgaaca
      541 cggtgagcag gccgtcgcgg gcggagatgt cgcccagcca gtggcgcaaa tcctcggtga
      601 tatcaatgaa gcccttgccg cgcgtccgca cccgtagcct gtggctcgct tggctgagtc
      661 cggccatcgc tcaggcgagc gccgcgcggg ggagatcgca cctcaggctc aaagctcctg
      721 ccccgcctct tgccgcttta ggtcgcgcac caagtcctgc aggctgcgga tcatcttgtt
      781 gatgaaatcg ttcatctcgc cgagggcaag cccgggaaat tgcgtcgacg ggttggccgg
      841 aagcggcccc gcctccgctt ccctctcttc cgccttggcg agcttgtcct tgagctcgcg
      901 gttctcgcgg gcgaggcgat cggcctccgc tttgagcgca agcgcgtcgt cggcgacgct
      961 ctcgcacgcc caatggtcga gcttctggcc gcacaccgac atcgcgccgg tggcactgtc
     1021 gagccgccag acgctgtcac cgacggtggt catggtgtag cggcctgggt cctcgccggc
     1081 gtagcagggc gaaacggcaa aaaggacgaa agcaagggcg agcagtctcg tggtcatggc
     1141 ccctcccctc ctctggcggt cgatagtcct gccccctcgc aagcttggca agtcccacga
     1201 ttctcgcgac aaatgtccca caacagggcg ctgcttaacc caaaatttac cgcgacttgc
     1261 cacggtggtg aggaccgttc ggtggcgcgt ctccctagaa gagcaacccc acgattcagg
     1321 cccatcgaac agggcatcca ttttcgcagg ggaacgggat ggctccggaa gtacgttggc
     1381 aagcgagcag cgccgccggt tcaagcggca gcgaaacccg tgcgcccgac cgggagagcg
     1441 tcgagagcct gctgcgcaag ctgatcaagc ggatcgagga gagcgagcgc cgctatgaag
     1501 aggcgctgga cgagttgcac gcgcggctcg gccaggtctc gtacagcgca agcgtcaacg
     1561 aggctcttgg caccccggaa gagaccgaga ctctggagcg gctgcggcgt cagctgagcg
     1621 acctcgcgcg ccggctggag caaccgcaac cgaaaaagga ggccgaacgg cttccccggc
     1681 tggacaaggc cctcgatgag gtccgcgccg tgtccacggg tcttgctttg gccgagccgg
     1741 actggttcgc atcgctgtcg gcctcgctag ccgcggccga ggggcgagcc gagcctgagc
     1801 ccgtgcctgc cccattgccg gcgagcgagc cgcgttacac ctcgcggtct tttgcgcctt
     1861 cgccttctaa tgacgaggat gccgacttcg accgccgttt gatcgagatg gctcagcggc
     1921 tcgagcgctc gatcggcgaa gcgttgccgt cctcgtcgat cgaaaagctc aatgcccgca
     1981 tggaggagat ctcggcgcgc ttcgagacgg cccttgcgca agagccaaag ctcgagacgc
     2041 tgcaccatat cgagcggcaa gttgccgaca tggggttcca gcttggccgc gtcgagcagc
     2101 aggccgcgcg aatcggcgcg gtcgaaggcc agttgcaacg gctggtcgag cgcatcgaat
     2161 cgacgccggc tcagatcgag cggacggcaa caaaggcggc gcaggagacg gcgcggctcg
     2221 tcagcgagac gggacttggc aagccgagcg ctgccgagcg gctcgattcc ctccaccgcg
     2281 acatcgtggc catgaatgag cgcaaccttg ccaccgacga ccgcctcgtc gacacgctta
     2341 cggcgatgca cgagtccttg aaagggctca tgcagcggga gcgcgacaga gccgcggcaa
     2401 cggccgcgcg cgaggctccg ccgcgcgtgg agcctgccgc aagcgaggcc ggccctgccg
     2461 atttcgctca aagccgccag cccgtggcgg ccgcacagat ccaagcggcc gagccgccac
     2521 gccagcgcac caccttcatt cgggatattc ccttcgagtc gaccgaggat ctggtcgctg
     2581 ccgcccgccg cgccgcgcaa gccgctgcgg cgcgggcaga ggaacggaac gccctgcccc
     2641 agcgaaaggt gcgagccaca gccgaaacca agccaagcga ggagccggga cggcacaagg
     2701 tctcacttct catggtggcc gctgcgatcc tgctcatgat cagcgccgcc ttgctcctga
     2761 ccaggctcaa gttgaaacca gattttgacg gtcctccacc ggcggccgag cagagcatgc
     2821 ccgcacccgc cgccgaggcg cctcccttgc ctgacgcgcc gctccagacc gaagtcattc
     2881 cgtcgcccga gggcgagtcc atgccctccg gcgcggcggc acctgaagtt ctcccggagc
     2941 cggaagcgcc tgatgccgca cccaccgcac ctcacgccgt cacgccgtca ccaagcccgg
     3001 acgggacggg agcgaggtcc ccggtgcacc tcggcgacgg agccgatgaa gcccttgtcc
     3061 agccagtgtc gctgagccca tcgggcggcc gaagcctgcc tcccggcgtc agcgtgatgg
     3121 tcgtcgagcc ctaatttcgc gacccaagcc cgaggtcttg tcgcggcccc ccgacagcag
     3181 accttcgtct gattttttag ccacctccga tgacaaattg gtaaggcggg tggttcgcgc
     3241 attgacaggg gcggttcccg cgaacacctt gcaaatagcg cgaaaatcaa cgcttccggc
     3301 ccaatgctcc aggtctatct gcccgtcgcc gagatctcgg tgaatctcct ggttatgctg
     3361 ggccttggtg cggccatcgg ctttctctcc ggcatgttcg gcgtaggcgg aggcttcctg
     3421 ctcaccccgc ttctgatgtt ctcaggcatc ccaccggcca tcgccgtggc gaccggcgca
     3481 aatcagatcg tcgcctcctc ggtgtcaggg ggtctggtgc aatggcgccg cggcaatatc
     3541 gacttccgca tgggctgggt gctcatcatc ggcggcgttt tcggcgccgt tgccggcgtg
     3601 ctcctcgtcg gactgctgag caggctcggc catgccgacg tcttcatctc gctcgtctac
     3661 gtaaccttcc tcggcgtcat cggatcgctc atgctggttg agagcgtgag cgccatccgg
     3721 cggacgcgcg ccggcctgcc ggggagagcg cggaagccag gcgagcacac ctggatccac
     3781 ggactgccct tcaagatgcg cttcccgcgc tcgcgcctct atatcagcgc cattccacct
     3841 ctgatccttg gggccatcgt cggcctgctc gcctcgatcc tcggcgtcgg cggcggtttc
     3901 attgtcgtgc caggcatgat ctacctcctg cgcatgccga ccaacgtggt catcggcacc
     3961 tcgctgttcc agattatttt cgtgacggcc gtcgtcacca tcctctatgc agtgttgaac
     4021 cactcgctcg acatcctgct cgccctgctt ctcgtgatcg gcggggtgat cggcggattg
     4081 ttcggcgccg atgccggtca gagattgaag ggcgaacagc tgagactttt gcttgcggtc
     4141 atggtgctcg ccgtggcgct tcgcttgctc gtcggcctcc tggtgccgcc cgaggacctc
     4201 tacagcctcg ggcgagtcgt gacgggggca tagaccatgc agccggcacg cctgctcgcc
     4261 cttcttgcgg ttgcgctcct gccggggcgg caaactttcg cggctgagcc gccaagagcc
     4321 gagcaagtcc agtcggacgt ctccacacgg gagatctcga tccaatcgaa cttcaccggc
     4381 gtcgagatcg tgctgttcgg ctccatcgac ttcacccgcg cgccttcgct ggacgaaggt
     4441 ccctacgacg tcatcatggt catccgcggg ccaaaccggc ccatcgtcgt ccggcgccag
     4501 gaacggatcg cgggactttg gatgaacggc gcgtcggaga cgttcccctc ggtgccaggt
     4561 tactatgccg tgctcgcctc gcggccgttc agggcggtgg cctcggaagc tgtcctcaag
     4621 aacctcggca tcggcttctc caatctcgat ttcggcaaga ccgccgaggg cgagagcgaa
     4681 gctgacgggt tccgcgcaag cctgatccgc ttgcaagagg aacatgacct gttccaggag
     4741 tccgacgacg cgatcagttt tatcggccgc agcctgtttc gtggaagcgt ccaacttccg
     4801 gtcaacgtgc cgatcggccg ctacaccacg caagtgttcc tgtttcgcga cggcaagctc
     4861 ttaagccaga gccagaacag cctgcaggtc gaaaaagtcg gcgttgagcg cgtggtctac
     4921 atgctcgcct tccactatcc cttcgcctac gggctgctgg cggtgctgat ggcggtgagc
     4981 gcggggcttc tcgcctggac ggtgttcagg cgcgagtgac gactcaggga atcgtcagtc
     5041 ggggagcagc aggctcgcct gcaagggata gacgctcgtc cgtcccgtga gccacgcctg
     5101 catcagatgc cagtggaaca aggacgagaa ggcctcgtcg agcacgttga gcccgccggc
     5161 ataagcgccg aaggccggca tcaccaggcg gtggccgtcg gtggcgaagc atttccgcct
     5221 gatcccctcg ccacgtctcg caatgcgcgc caccgggtgc agatggccgg cgatctcagg
     5281 ggcgcggctg agcgagggct ggtggcacag ggtgatcccg ccgatcatca gcgaccggca
     5341 gacaaagccg ccgatgcttt cgggcagatc agggtcgtga ttgccgagga tccagtacca
     5401 gtcgcggcct tgctgcagcg ccgccaattc ctcgcgatcg tcggcgctaa gccgtccggc
     5461 gagctcgctg cggtgaaagc tgtcgccgag cgcaaccaca cggcggggtg agaaatggtc
     5521 gaggcaggcg gcaaggcgct gcaaggtgct ccgcgtgtcg taagggggga gcatcatgcc
     5581 gcgcgccgcg aaggctgcgc ccttctccag atgcaaatcg gcgacgagca gggtatcctc
     5641 ggactcccag tagagggcgc cggccgcgac cggcaagaac gaatggccgg caagtctgat
     5701 gggcggccgc cttccggcga gaacttcaga catcaagcgc agcatggtcc cccatcgcct
     5761 ccgctatcag atcttccact gcctcgcgca acagcgcttc gctcgcgtcc cccggcatgc
     5821 tttcgcggcc aatctcgagc atggccggca ccgcaagcgg cgagacccgg tctagcgcaa
     5881 tatgcctgat tcttccgcgg atccgctgca aaaagccgct caggcgtccg acatcgatca
     5941 ggccggttgc ggcatcggcg aaggcagcct gaaggagaat gtggtcaggc tcgtgctggc
     6001 gaagcacggc atagatcaga tcggaggaga cggtcatctg cctgccggtc ttctcctgcc
     6061 ccggatggcg gcgctcgatt agccctgaga tgatggcgca gacgcggaag gagcgtttca
     6121 tcaggctcga ttcggcaagc caggcatcga gatcgtcgcc gagcatgtcc tcgtcgaaca
     6181 gtttggcgag gtcgagccgc cccgtccgca ccatctcgcc cgtatcggcg agcccccaga
     6241 cgcagagcgc atattcattg gcgacgaagc ctagcggctt ggcatgggcg cgctcgagcc
     6301 gccgcgtgag caacatgccg aggctttgat gggcgagccg tccctcgaag ggatagcaga
     6361 caagataatg cctttgcccg cgcggaaagg tctcgatcag gatctcgtcg gggccgggca
     6421 cgaccgacac ctcgctctga atgcgcagcc agtccgagac ctgatcagac agccgcgccc
     6481 aatggcgccg gtcggcaagc agccgccgca cgcgatcggc gaggtaggtg gagagcggga
     6541 acttgccgcc gtcataggag ggcaccttgg gctcgggcgc gtggctgcgg gtgacgatgg
     6601 cgtcgggctc gcgcaagcct tcgaagcgca gcacttcgcc cgcaaacacg aaactgtcgc
     6661 cgggcatgag ttgctcgatg aaatattcct cgacctctcc gagcacgcgg ccggcggcaa
     6721 gaaccgcaga ggaccttcgt gcgccgccct gacgccgggc gagccgcacg cgcaccatgg
     6781 gagcctcgac gatggtgccg acattcaggc gatattgctg ggcgagccgc ggatgggcga
     6841 tgcgatagcg cccgtcaggc atctgtttca gcttcgcgta gcgctcgtaa ttggcgagcg
     6901 cgtagcctcc ggtggaaacg aagcgcacga tcttgtcgaa gccttcgcgc gtgaggccct
     6961 gatagggaga ggcgctggtc acctcgcggt aaagctgatc gggatccaac ggtcccgagc
     7021 aggccatgcc gagcacgtgc tgggcgagca cgtcgagcgc gcctggccgc gacgggggcg
     7081 tgtcctgtgc accctcctct gccgcctcaa gcgcagcctg gcattccacc acctcgaagc
     7141 ggttcgccgg cacgagcaga gccttgctgg gctcatcgag ccggtgattg gcgcggccga
     7201 tgcgctgggc aagcctgctt gcacctttcg gcgcgccaac attgatgacg aggtcgacgt
     7261 cgccccagtc gatgccgaga tcgagcgtcg aggtgcacac cacggcgcgg agcttgccag
     7321 ccaccatcgc cgcctcgacc ttccgccgct gcccgacatc gagcgagccg tgatgcagcg
     7381 cgaggggcaa attcgcatcg ttgtagcgcc acagcgcatg gaacagcatc tcggcttgcg
     7441 agcgcgtgtt gacgaagatg agcgacatgc ggtgggcctt gatcgcctcg tagatctcgg
     7501 gtagcgcata gcgcgccgag tgcccggccc a
//
LOCUS       DATNAE010000203         2610 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2598750, whole genome shotgun sequence.
ACCESSION   DATNAE010000203 DATNAE010000000
VERSION     DATNAE010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2598750"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="VN518_04660"
     CDS             <1..752
                     /locus_tag="VN518_04660"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HXE03898.1"
                     /translation="AFHGDCRAGGLLAFSGSPIYLPMCSRYSLTSPPEAVRATFAYRD
                     TPNFPVRYNIAPTQDIGVVCVDADGQRRFRMMRWGLLPSFVRDPKAGPPMINARAEGI
                     STKSAFRSAFAERRCLVPADGFYEWTGPKGARRPFLLRPRKGGLIAFAAIWERWRDRA
                     GGEIDSVAIITCDANATVTPLHDRMPVVLGLEHFEAWLDCKKAELKAAQDMLQPAPDD
                     LLEAIEMHPMINDSRRDEPGIQQPLQTQLLL"
     gene            complement(754..2193)
                     /locus_tag="VN518_04665"
     CDS             complement(754..2193)
                     /locus_tag="VN518_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009465640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HXE03899.1"
                     /translation="MTSDQEPCRRPSDETLDALVRIVGVDHAVRDPEAMTPYLVEWRD
                     RYFGNAALVLKPGSTAEVAAILKRANETRTAIVPQGGNTGLVGGQIPFESGHEVVVSL
                     SRMTHVRDIDLDGNTMTVEAGLVLANAQAVAASAGRLFPLSLASEGSCQIGGVLATNA
                     GGMAVLSYGSARDLTLGLEVVLADGSVWNGLKRLRKDNSGYDLKDLFIGSEGTLGIIT
                     ATVLRLYPKPGETVTAFAGLSHLESAIAFFSRAYAFAGPALTAFELMPRLGLDFVLRH
                     VGDARDPLRIPHAWYVLLELSSPREGDDIARIAETLLGEAAEAKEIDDAVIAASLAQE
                     RELWRLRELLSEVQKHEGGSIKHDVAVPVARVPEFIARASQLVELMIPGARPVPFGHL
                     GDGNIHYNVSQPSSMDRAVFLANWEALNAAVHEIVLDLGGSISAEHGIGRLKRDLLRR
                     AKAPLELEMMRKIKQAFDPNGILNPGKLL"
     gene            complement(2190..>2610)
                     /locus_tag="VN518_04670"
     CDS             complement(2190..>2610)
                     /locus_tag="VN518_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Sua5 family C-terminal domain-containing
                     protein"
                     /protein_id="HXE03900.1"
                     /translation="VTLLRLGAIPRQEIETVLGRKLAAPEAGSQVTSPGQLATHYAPV
                     TPLRLDAGKPRRGEAFLAFGPNAPSFSGPAINLSRRGDLVEAAANFFAALRTLDEAGV
                     ETIAVMPIPDHGLGEAINDRLKRARIPVTLTASADLP"
BASE COUNT          453 a          842 c          861 g          454 t
ORIGIN      
        1 gggccttcca cggcgactgc cgggccggtg gcttgctcgc cttttccggc agccccatct
       61 atctgccgat gtgctcccgc tacagcctca cctctccgcc ggaagccgtg cgcgccacct
      121 tcgcctatcg cgacacgccg aattttccgg tccgctacaa tatcgcgccg acgcaagaca
      181 tcggtgtggt gtgcgttgac gccgacggtc agcggcgctt ccgcatgatg cgctggggcc
      241 tgttgccgtc tttcgtcagg gatccgaaag ccggccctcc catgatcaat gcgcgagcag
      301 agggcatatc gaccaaatca gccttccgca gcgccttcgc cgagcggcgc tgcctcgtgc
      361 cggctgacgg tttctacgaa tggaccgggc ccaagggcgc gcggcgaccc tttctgctcc
      421 gcccgcgcaa aggcggcctg atcgcctttg ccgccatttg ggagcgctgg cgggatcggg
      481 ccggcggcga gatcgactcg gtcgccatca tcacctgcga cgcgaacgcc accgtcacgc
      541 cgttgcatga ccgcatgccg gtggtgttgg ggctagagca tttcgaggcg tggctcgact
      601 gcaagaaggc ggagctgaag gccgcgcaag acatgttgca gccggcgccc gacgatctcc
      661 tcgaggcgat cgagatgcat ccgatgatca acgattcgcg ccgcgacgag cccggcatcc
      721 agcagccgct gcagacgcaa ttgctattgt aagctagagc aacttgcccg gattaaggat
      781 gccgttcgga tcgaacgcct gcttgatctt gcgcatcatc tcaagctcga gcggcgcttt
      841 cgcgcgacgc aggagatcgc gcttgagccg gccgatgccg tgctcggcgc tgatagagcc
      901 gccgagatcg agcacgattt catgcaccgc ggcgttgagc gcttcccaat tggcgaggaa
      961 tacggcgcga tccatcgaag aaggctggct cacattgtag tggatgttgc cgtcgccgag
     1021 atggccgaac gggaccggcc tcgcgccggg gatcatcagc tcgacgagct gactggcgcg
     1081 ggcgatgaac tcagggaccc tggcgaccgg caccgccacg tcgtgcttga tggagccgcc
     1141 ttcgtgtttt tgcacctcgc tcagcagctc gcgcaggcgc caaagctcgc gctcttgcgc
     1201 aagcgaagcg gcgatgacgg cgtcgtcgat ctccttggcc tctgcggctt cgccaagcag
     1261 tgtttcggca atgcgggcga tgtcgtcgcc ttcccgcggg ctcgaaagct ccagcagcac
     1321 ataccaagcg tgcgggatgc gaagcggatc gcgggcgtcg cccacgtgtc tcagcacgaa
     1381 gtcgaggcca agccggggca tcagctcgaa agcggtgagc gcaggtcccg caaaagcata
     1441 ggcgcgggag aagaaggcga ttgcggactc aagatgagaa agcccggcga acgccgtcac
     1501 cgtctcgcca ggcttcgggt agaggcgcaa caccgtggcg gtgatgatgc cgagcgtacc
     1561 ctcagagccg atgaacaagt ccttgaggtc gtagccgcta ttgtccttgc gcaaacgctt
     1621 gagcccattc cacacgctgc cgtcggcgag caccacttcg agaccaaggg tgagatcgcg
     1681 ggcgctgccg taggacagca ctgccatgcc gccggcgttg gtggcgagca cgccgccgat
     1741 ctggcagctt ccctccgagg cgaggcttaa gggaaacagc cgcccggcgc tcgccgccac
     1801 tgcttgcgca ttggcgagca caaggcctgc ttcgaccgtc atggtgttgc cgtcgaggtc
     1861 gatatcgcgg acgtgggtca tgcggctcaa gctcaccacc acctcatggc cgctttcgaa
     1921 cgggatctgg ccgccgacca gcccggtatt gccgccttgc ggcacgatcg ccgtgcgcgt
     1981 ctcgttggcg cgcttcagaa tcgcggcaac ttcggcggtc gagcccggct tgagcacaag
     2041 cgcggcgttg ccgaaatagc ggtcgcgcca ttcaacgaga taaggcgtca tggcctcagg
     2101 gtcgcggacc gcgtggtcca cgccgacgat gcggacgagc gcgtcgagcg tctcatcgct
     2161 cggccgcctg cagggctctt ggtcggacgt catggaagat cggcgctggc ggtcaaggtt
     2221 acagggatgc gcgcacgctt gagccggtcg ttgatggctt cgccaaggcc atgatcgggg
     2281 atcggcatca ccgcgatcgt ctctacgcca gcttcatcga gcgtcctgag cgcggcgaag
     2341 aaattcgccg cggcttcgac gagatcaccg cgccggctga gattgatcgc agggccggag
     2401 aagcttggcg cgttcgggcc gaaggcgaga aacgcttctc cgcggcgcgg tttgcccgcg
     2461 tcgaggcgaa gcggcgttac cggcgcataa tgggtggcga gctgacctgg cgaggtcacc
     2521 tggctgccgg cttcaggcgc cgcaagcttg cgcccaagga ccgtctcgat ctcctgccgc
     2581 ggaatggcgc ccaagcgaag caaagttact
//
LOCUS       DATNAE010000204         2852 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3803606, whole genome shotgun sequence.
ACCESSION   DATNAE010000204 DATNAE010000000
VERSION     DATNAE010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3803606"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="VN518_04675"
     CDS             complement(<1..552)
                     /locus_tag="VN518_04675"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008596193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HXE03901.1"
                     /translation="MYRYDEFDHAFVHERVSQFREQVERRLAGKLTEDEFRPLRLRNG
                     LYLQLHAYMLRIAIPYGVLSPRQLRQLAMIGRRFDRGYGHFTTRQNLQFNWVKLVDVP
                     EILSALADVEMHCIQTSGNCVRNVTADPFAGAVNDELEDPRPWCEVLRQWSTLHPEFS
                     WLPRKFKIAVSGAAEDRAATGFHD"
     gene            complement(539..832)
                     /locus_tag="VN518_04680"
     CDS             complement(539..832)
                     /locus_tag="VN518_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012109765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2849 domain-containing protein"
                     /protein_id="HXE03902.1"
                     /translation="MPNVVTANRLIDGIVVYLAPDGGWVEELARAKLADTEDETKALE
                     SEAAKAVAERRVVAVYPMEVALNDGVPVATSVRERIRAAHRTTLTKDWYDVPL"
     gene            complement(825..2255)
                     /gene="cysG"
                     /locus_tag="VN518_04685"
     CDS             complement(825..2255)
                     /gene="cysG"
                     /locus_tag="VN518_04685"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF007922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HXE03903.1"
                     /translation="MRTFPIFVSVDRKPPLVTGGGELAAIKARLLLKRAETVDIAADR
                     LGPELAELERAGRVALIAAQPGVDQVRGRPLVIAATGVDAEDARVAAIARALGVPVNV
                     PDKPALCSFVMPAIVDRGEVTVAIGTEGTSPVLAQRLRALLECELHPRLDALARLAGD
                     FRDAVAEKIPAGRPRRKFWEGVFDGAASEAVLAGEESEARCLVGEAIEAAVKAQAVRG
                     RVLLVGAGPGDPELLTLKAVRALKAADVILYDRLAGTGVLEHARREVELIPVGKAKGE
                     HSVPQDKIQELMIERARAGQTVVRLKGGDPLVFGRAGEEIAALREAGIEVEIVSGITA
                     GLAAAASLQIPLTHRDISHSVTFVSGHEAGGDEPSFEHLDLAALGKGESTLLVYMGVS
                     TAGLIASRLLAAGWKPECPVIAVENASRDTERRVATTLAELAATPERLELKSPAVLIF
                     GEVAGLPASGLVEDLLLLTELRRAYA"
     gene            complement(2262..2612)
                     /locus_tag="VN518_04690"
     CDS             complement(2262..2612)
                     /locus_tag="VN518_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HXE03904.1"
                     /translation="MFVVVRMPDGSSHRLEALDGWRVMEVIRDWGLPIKAECGGACAC
                     ATCHVWVDEKWLPKLTAPTHEELEMLDGAFHVDERSRLCCQIIMSPELDGLELELAPE
                     SLADAPPARLAVGG"
     gene            complement(2614..>2852)
                     /locus_tag="VN518_04695"
     CDS             complement(2614..>2852)
                     /locus_tag="VN518_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016920645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HXE03905.1"
                     /translation="LNENLIPVDTEKFETGTKAIFAIGDINTYPGKLKLILSGFHEAA
                     LMAQAAHRYVHPDKPLRFQYTTSSSELQRLLKVA"
BASE COUNT          506 a          907 c          972 g          467 t
ORIGIN      
        1 gtcgtggaag ccggttgcag cacggtcctc tgccgcaccc gatacggcga tcttgaactt
       61 gcgcggcagc cagctgaact ccggatgcag cgtcgaccat tggcgcagaa cctcgcacca
      121 ggggcgcggg tcttctagct cgtcattgac ggcgccggcg aaaggatcgg cggtaacgtt
      181 gcgtacgcag ttgccgctcg tctgaatgca atgcatctcg acgtcggcga gcgcgctcag
      241 gatctccggc acgtcgacca gcttcaccca gttgaactgc agattctgcc gcgtggtgaa
      301 atggccgtag ccgcggtcga agcggcggcc gatcatggcg agctgacgca actggcgggg
      361 cgagagcacg ccatagggaa tggcgatgcg cagcatatac gcgtgcagct gcagatagag
      421 tccgttgcga agccggagcg gacggaactc gtcctcggtc agcttgccgg caagccggcg
      481 ctcgacctgc tcgcgaaact gcgacacacg ctcatgcacg aaagcgtggt cgaactcgtc
      541 atagcggtac atcgtaccaa tccttggtca gagtggttcg gtgggcggcg cggatgcgct
      601 cgcgcacgga cgttgcaacc ggcacgccgt cgttgagcgc gacctccatg ggatagacgg
      661 cgacgacgcg ccgttcggcg acggccttgg cggcttcgct ttccagagcc ttggtctcgt
      721 cctcagtatc ggcgagcttg gcgcgggcaa gctcctcaac ccagccgccg tcgggggcga
      781 ggtagacgac gatgccgtcg atcaggcggt tggcggtgac gacgttaggc ataggcgcgc
      841 ctcaactcgg tcagcaacag gaggtcttcg acgaggcctg aggctggaag cccggcgacc
      901 tcgccgaaga tgaggacggc agggctcttc agctccaggc gctcaggtgt tgcggcaagc
      961 tcggcaaggg tggtggcgac gcggcgctcg gtgtcgcggc tcgcattctc aacggcgatg
     1021 accgggcatt cgggcttcca gccggccgca agcagccgag acgcgatcag cccagcggtc
     1081 gagacgccca tatagacgag gagcgtgctc tcgcccttgc cgagtgctgc gaggtcgaga
     1141 tgctcgaagc tcggctcgtc gcctccggct tcgtggccag agacgaaggt cacgctgtga
     1201 gagatgtcgc gatgggtgag cgggatctgc aaggacgccg cggcagcgag cccggcggtg
     1261 atgccggaca cgatctcgac ctcgatgcca gcctcgcgca aagcggcgat ctcctcgccg
     1321 gcgcggccga acaccagagg atcgccgcct ttgaggcgca ctacggtttg ccctgcgcgc
     1381 gcccgctcga tcatcaattc ctggatcttg tcttgcggga cgctgtgctc gcccttcgcc
     1441 ttgccgactg ggatcagctc gacttcgcgg cgggcatgct ccagcacgcc ggtgccggct
     1501 aggcggtcgt agagaatgac gtcggcggct ttcagcgcgc gcacggcttt cagggtcaag
     1561 agctcgggat cgcccgggcc ggcgccgacg aggagcacgc ggcctcgcac ggcttgcgcc
     1621 ttcaccgcag cttcgatggc ttcgccaacc aggcagcgtg cttcgctctc ttcgccggcg
     1681 agcactgcct cgctcgcggc gccgtcgaac acgccttccc agaatttgcg ccgcggacgc
     1741 cccgccggga tcttctcggc gacggcgtcg cggaagtctc cggcaagacg ggccagagca
     1801 tcgagacgcg gatgcagctc gcactcgagc aaggcgcgca ggcgctgggc gagcactggc
     1861 gaggtgcctt cggtgccgat agcaaccgtc acctcgccgc gatcgacgat ggccggcatg
     1921 acgaagctgc acaaagctgg cttgtcggga acgttgaccg gaacgccaag cgcccgtgcg
     1981 atcgcggcga cgcgcgcgtc ttcggcgtca acgccggtcg cggcaatgac caaaggccgg
     2041 ccgcgcacct gatcgacgcc aggctgtgcg gcaatgagag cgacgcggcc ggcgcgctcg
     2101 agctcggcaa gctccggccc aagccgatcg gcggcaatgt cgacagtttc ggcgcgcttc
     2161 agcaggagcc gcgcctttat ggcggcaagc tcgccgccgc cggtcaccaa aggcggcttg
     2221 cggtcgacgc tgacgaagat ggggaaggtc cgcatcggtt ctcaacctcc aaccgcaagg
     2281 cgcgccggcg gcgcatcggc aaggctctcg ggcgcaagct cgagctcgag cccgtcaagc
     2341 tcaggcgaca tgatgatctg gcagcacagg cgcgagcgct catcgacgtg aaaggcgccg
     2401 tccagcattt cgagctcctc atgggtggga gccgtgagtt tcggcagcca tttctcgtcc
     2461 acccagacat ggcaggtggc gcaagcgcag gcgccgccgc attcggcctt gatggggagg
     2521 ccccagtcgc ggatcacctc catgacccgc caaccatcca gcgcctcgag gcgatgggag
     2581 gaaccgtccg gcatacgcac gacgacaaac atgtcaggcc accttcaaca gacgctgcag
     2641 ctcggacgag gaggttgtgt actggaagcg caaaggtttg tcaggatgga cgtagcgatg
     2701 ggcggcttgc gccatgagcg cagcctcatg aaacccggaa aggatgagtt tcagcttacc
     2761 aggataggta ttgatgtcgc cgatggcgaa aatggccttg gtgccggtct cgaatttctc
     2821 ggtgtcgacc gggatcaagt tctcgttgag at
//
LOCUS       DATNAE010000205         5216 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_799771, whole genome shotgun sequence.
ACCESSION   DATNAE010000205 DATNAE010000000
VERSION     DATNAE010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5216
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_799771"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VN518_04700"
     CDS             <1..306
                     /locus_tag="VN518_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03906.1"
                     /translation="ASPQSAWRTDGAILVLKVDGPRAIEVQHRAEQILERVNTYFGYR
                     AVAQLRFLQAPLGKAKGQAPASAGAEEPLPAHGIADTGLAKALGRLAAGIRGKTPGP"
     gene            394..1158
                     /locus_tag="VN518_04705"
     CDS             394..1158
                     /locus_tag="VN518_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002970967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HXE03907.1"
                     /translation="MTETTEKAAPNRTLYIVLGLVLAVAVAAGVFYWRGGFAQSGARV
                     AEKDKVDPDVAVLMEPGPLPDIVIGSASAPNTIVEYASMTCPHCALFQTEVFPQLKTK
                     YLDTGKARYMLREFPLDNLAAAAFMLARCAGDDRYYAMVDAMFSTQESWAMPGVDAKD
                     KLLQIARQAGISKEQFDQCLANKELFGKIVKTREIANEKFQVDSTPTFFVNGKRLKGD
                     HQLKDFDVALGEAPAPAPADADKGKTEGSTPPPTGQ"
     gene            1181..1837
                     /locus_tag="VN518_04710"
     CDS             1181..1837
                     /locus_tag="VN518_04710"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HXE03908.1"
                     /translation="MRRPTLLLLFAAGVALSGCGGGVGPTADAAADKALNGPPIAELM
                     ADGPLGERVLGSPNAPITVIEYASLTCPHCAHFQKEVFPRVKKELIDTGKIRFIVREF
                     PIGHTSGTAAIINRCAPEDKYFSLFNAFLLRQPEWVSQEVRPDAIYSVAKSSGMSRET
                     FDKCLTNQTIIDGLAEVKQRGRKFDVVGTPTFFANGRKAQGTITFEEFKALLGPQSST
                     "
     gene            1903..>5216
                     /locus_tag="VN518_04715"
     CDS             1903..>5216
                     /locus_tag="VN518_04715"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXE03909.1"
                     /translation="MNITRLRLLGFKSFVEPTEILIEPGLTGVVGPNGCGKSNLLEAL
                     RWAMGETSYKSMRGSAMDDVIFAGSQHRPSRNTAEVTLFIDNSARTAPAEFNDADDLE
                     VTRRIEREVGSAYRVNGKDARARDIRLLFEDAATGARSPALVRQGRIGEIVNSAPQER
                     RRVLEDAAGIAGLHSRRHEAELRLKATEANLERLGDLIGELTTQLQALKRQARQAQRY
                     REISGKIRETEALQHHLHWVSACAHVEQEEAAFQSALGMVAQETKAEGEALRQQAEAA
                     EKLQPLRDEEAARAAVLHRLSVERDALEREEARAKARQAELETRLAQASRDLAREEEH
                     IAEAEGQLRRIEHETRRLAEAGSRDHEEPQLRQTAAIAANALSAAEAAFSAATEDLAR
                     ARAERRRIDVEQGEFEKRIALLIRQSGDIAEELAKLETSSGGETNLVALNTDLARLSG
                     LVETLEAELAASEASEEAAQAREAESRAAAASAKLRVQELHTELATLLKLLRPDEAGA
                     WMPIVDDVRIALGYEQALGASLGDDLDAAGDEAAPSHWRLVPSEGDPELPAGATALSQ
                     FVSGPPSLKRRLAQIGVVERAQGKALQARLKPGQRLVSTEGDIWRWDGYTVAAGTASA
                     AGARLVERSRLEALKAEADRAEATLQDAEAEAATLAGNAHAAIEATKALRLKVRQARD
                     ELGRTRDAIAAAEHESLANSKQLGALAEAKSRTLVGLDEARRALARTLSARHELAALD
                     VLEAVLERAHTDAAEKRSAHARAEAAVQGFEREVRLRQERLEAISAERELWQNRIAKA
                     REQIETLRAREAETRADLTALANLPAEIEQRRGRLLDAITAAERERGKAADDLAIAET
                     ELKRREKALREVQEKLAGAREARARSQALLESARERRSEVAHAIREQLDCAPEACLAL
                     AGMKPGAPLPSLAEVEGRLLKLRGERERLGGVNLRAEEEEADISTRLEEMEREKADVE
                     QAIARLRQGISNLNREGRKRLLEAFDSVNAHFQRLFQTLFGGGTAELQLVDSDDPLES
                     GLEIMACPPGKRPQVLTLLSGGEKALTALALIFAVFLTNPSPICVLDEVDAPLDDAKV
                     ERFCLLMEEMAKETDTR"
BASE COUNT          949 a         1740 c         1737 g          790 t
ORIGIN      
        1 gcctccccgc aaagcgcctg gcggaccgat ggcgccatcc tcgtgctcaa ggtcgacggc
       61 cctcgcgcca tcgaggtgca gcatcgtgcc gagcagatct tggagcgggt gaacacctat
      121 ttcggctacc gggccgtcgc ccaactgcgc ttcctccagg cgccgctggg caaggccaag
      181 ggccaggccc cggcctctgc aggtgccgag gagcctttgc ccgctcacgg cattgccgat
      241 accgggctag ccaaggccct tggacggctt gcggccggta tccgcggcaa gaccccggga
      301 ccttgagacg cgcttccgct tttctcgctt ctgccataaa gaactggcct gagaacggga
      361 aaccaccgga aagcaagcga gcggagtaaa ccagtgaccg agacgaccga aaaagctgcc
      421 cccaacagga ccctgtatat cgtgctcggc ctcgtgctgg cggtggccgt cgccgccggg
      481 gtcttttact ggcggggagg cttcgcccag agcggcgcca gggttgccga aaaggacaag
      541 gtcgacccgg atgtggcggt gctcatggag ccaggaccgc tccccgacat cgtcatcggc
      601 tcagcaagcg cgccaaacac catcgtcgag tatgcctcga tgacctgccc gcattgcgcg
      661 ctgttccaga ccgaagtgtt tccgcagctg aaaaccaaat atctcgacac cggcaaggcc
      721 cgctacatgc tgcgcgagtt cccgctcgac aacctcgccg ccgccgcctt catgctggcg
      781 cgctgcgccg gcgacgaccg ctattacgcg atggtcgacg ccatgttctc gacgcaagag
      841 agctgggcga tgcccggcgt cgatgccaag gacaagctcc tgcagatcgc ccgccaggcc
      901 ggcatctcca aggaacagtt cgaccaatgc ctggccaaca aggagctgtt cggcaagatc
      961 gtgaagaccc gcgagatcgc caatgagaag tttcaggtcg actcgacgcc gaccttcttc
     1021 gtcaacggca agaggctgaa gggcgaccac cagctcaagg atttcgacgt cgcgctaggc
     1081 gaggctcccg cgcccgcccc cgccgatgcc gacaagggga agaccgaggg gtcaaccccg
     1141 cctccgaccg ggcaataagc atccaggaat aggcgacaag atgagacggc ccacgctact
     1201 cttgctcttc gccgcggggg tcgcgctttc aggatgcggc ggaggtgtcg ggcccactgc
     1261 cgacgccgcc gccgacaagg cgctcaatgg gcctcccatc gcggagctga tggccgacgg
     1321 gccgctcggc gagcgggtgc tcggcagtcc caacgctccc atcaccgtca tcgagtacgc
     1381 ctcgctcacc tgtccccatt gcgcccattt ccagaaggag gtgttcccca gggtgaaaaa
     1441 ggagctgatc gataccggca agatccgctt catcgtgcgg gagttcccca tcggtcacac
     1501 ctcggggacc gccgccatca tcaatcgctg cgcgcccgag gataaatatt tctccctgtt
     1561 caacgccttc ctgctccgcc agcccgagtg ggtgagccag gaggtgcgcc ctgacgccat
     1621 ctattcggtg gccaaatcga gcggcatgtc gcgtgagacg tttgacaaat gcctcacgaa
     1681 tcaaaccatt atcgacgggc tcgcggaggt gaagcagcgg gggcggaaat ttgacgtggt
     1741 cggcacgccg accttttttg ccaatggccg caaggcgcaa ggcacaatca ccttcgaaga
     1801 gttcaaggct ctgcttggcc cgcagtcgtc gacttgatct tgcggcgccg cagatgcgtt
     1861 gaatcgcgcg tcgcttcttc caggtcctaa ggcaattcgc aattgaacat cacccgtctt
     1921 cgccttctcg gcttcaaatc ctttgtcgag ccgaccgaaa tcctgatcga gcccggactc
     1981 accggcgtgg tcgggccgaa cggctgcggc aagtcgaacc tgctcgaggc gctgcgctgg
     2041 gccatgggcg agacctccta taagtccatg cgcggctcgg ccatggacga cgtgatcttc
     2101 gccggatcgc agcaccggcc ttcccgcaac actgccgagg tgacgctgtt catcgacaat
     2161 agcgcgcgca cggcgccggc cgagttcaac gacgccgacg accttgaggt gacgaggcgc
     2221 atcgagcgcg aagtcggctc ggcctaccgg gtcaacggca aggatgcgcg ggcccgcgat
     2281 attcgcctgt tgttcgagga cgcagcgacc ggtgcgcgct ctccggctct ggtacgccag
     2341 ggccgtatcg gcgagatcgt caattccgcc ccgcaggagc gccgccgcgt gctggaagat
     2401 gccgcgggca ttgccggctt gcacagccgg cgccatgagg ccgagcttcg cctcaaggcc
     2461 acggaagcca atctcgagcg cctcggcgac ctgatcggcg agctcaccac gcaattgcag
     2521 gcgctgaagc gccaggcccg ccaggcgcag cgctaccgcg agatttcggg aaagatccgc
     2581 gagaccgagg ccttgcagca tcatctgcat tgggtctcgg cctgtgccca cgtggagcag
     2641 gaagaggccg cctttcagtc ggccctcggc atggtcgccc aggaaaccaa ggctgagggc
     2701 gaggcgctgc gtcagcaggc cgaagccgcc gagaagcttc agcctttgcg cgacgaggag
     2761 gcggcgcgcg ccgcggtgct gcatcgcttg agcgtcgagc gcgatgcgct ggaacgcgag
     2821 gaggcgcgcg ccaaggcacg ccaggcagag ctggaaacgc ggctggcgca ggcatcgcgc
     2881 gacctcgccc gcgaggaaga gcatatcgcg gaggctgaag gtcagcttcg ccgcatcgag
     2941 catgagacga ggcgtctggc ggaagcaggc tcgcgcgatc atgaggagcc gcaattgcgc
     3001 cagacggcgg cgatcgcggc gaacgcgctg tcggcggcgg aggccgcctt cagcgccgca
     3061 accgaagatc tggcgcgggc gcgcgccgag cgccgccgca tcgatgtcga gcaaggcgag
     3121 ttcgaaaagc gcatcgctct gctgatacgc caatcaggcg acatcgccga agagcttgcc
     3181 aagctcgaaa cctcgagcgg cggcgaaacc aatcttgtgg cgctcaacac cgatcttgcc
     3241 aggctgtcag gcctggtcga gacgctggaa gccgagcttg ctgcgtccga agcgagcgag
     3301 gaagctgccc aggcgcgcga ggcagagtcc cgcgccgctg ctgcgagcgc caagctgcgc
     3361 gtgcaggagc tccacaccga gcttgccacg ctcctgaagc tcctcaggcc cgatgaggct
     3421 ggcgcctgga tgccgatcgt cgacgatgtc cgcatcgctt taggctacga gcaggcgctt
     3481 ggcgcgagcc tcggcgacga tctcgatgcc gctggcgacg aggcggctcc ctcgcattgg
     3541 cggctcgtgc caagcgaagg cgatcctgag ctgccggcag gtgccacagc tttgagccaa
     3601 ttcgtctccg gcccgccttc gctcaagcgc cggctcgccc agatcggcgt ggttgagcgc
     3661 gcgcaaggca aggccttgca ggcaaggctc aagccgggcc agaggctcgt ctccaccgaa
     3721 ggcgatatct ggcgctggga cggctacacc gtcgctgccg gcacggcttc ggcggcgggc
     3781 gcccgcctcg tcgagcgaag caggctcgaa gccttgaagg ctgaggcgga ccgcgccgag
     3841 gcgacactgc aagacgccga ggccgaggcg gcaacgctcg ccggcaacgc tcacgccgcc
     3901 attgaggcga ccaaggccct ccgcctgaaa gtgcgccagg ctcgcgacga gcttggccgt
     3961 acccgcgacg ccatcgccgc cgccgagcat gagagcctcg ccaacagcaa gcaactcggt
     4021 gcgcttgccg aggccaagag ccggaccctg gtcgggctcg acgaggcgcg cagagccttg
     4081 gcgcggaccc tatctgctcg tcatgagctc gcggcgctcg atgtgctcga ggcggtgttg
     4141 gagcgcgccc acacggacgc cgccgagaag cgctcggccc atgcccgcgc tgaagctgcg
     4201 gtgcaaggct tcgagcgcga agtgcggctg aggcaggagc ggcttgaggc gatctcggcc
     4261 gagcgggagc tttggcagaa ccgcatcgcc aaggcgcgtg agcagatcga gaccttgcgc
     4321 gcgcgggagg ccgagacgcg tgccgacctt accgcgcttg ccaatctccc ggcggagatc
     4381 gagcagcggc gcgggcgtct gctcgatgcc atcaccgccg ccgagcgcga gcggggcaag
     4441 gccgccgacg atttggcgat tgccgagacc gagctcaagc gccgcgagaa agccttgcgc
     4501 gaggtgcagg agaagctcgc cggcgcgcgc gaggcgcggg cgcgcagcca ggctctgctt
     4561 gaatcggcgc gcgagcgccg gagcgaggtc gcccacgcca ttcgcgagca gctcgattgc
     4621 gcgccggaag cctgtcttgc gctcgccggc atgaagccag gcgcacccct cccctcactt
     4681 gccgaggtcg aggggcggct cttgaagctc agaggcgagc gcgagcggct tggcggcgtc
     4741 aatctccgcg ccgaggagga agaggcggac atctccaccc ggcttgagga aatggagcgg
     4801 gagaaggccg atgttgagca ggcgatcgcg cggctccgcc agggcatctc caatctgaac
     4861 cgcgaaggcc ggaaaaggct gctcgaagcc ttcgacagcg tgaacgcgca tttccagcgc
     4921 ttgtttcaga ccctgttcgg cggcggcacc gccgagctgc agctcgtcga ttcggacgat
     4981 cctttggagt ccgggctcga gatcatggcc tgccctcccg gtaagcgtcc gcaagtgctg
     5041 acgcttctgt cgggcgggga gaaagcgctc accgcgctcg cgctcatctt cgccgtgttc
     5101 ctcaccaacc cttcgccgat ctgcgtgctc gacgaggtgg acgcgccgct cgacgacgcc
     5161 aaggtcgaga ggttctgcct gctgatggag gagatggcca aggagaccga cacgcg
//
LOCUS       DATNAE010000206         2411 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_197366, whole genome shotgun sequence.
ACCESSION   DATNAE010000206 DATNAE010000000
VERSION     DATNAE010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197366"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1047)
                     /locus_tag="VN518_04720"
     CDS             complement(<1..1047)
                     /locus_tag="VN518_04720"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXE03910.1"
                     /translation="MAWKLNRRTFLWGTALTTLPWAPAIAQAAPARIGLLTVKTGPLA
                     QGGIQMEQGITAYLKEKNNTLAGRKVELVVGDTGGNPAGAKTKAQELVERDHVDFIVG
                     PLAAFELLAISSYVEQAKVPMLSIAAAEDMTQRKPNPYFVRASATSSQVNQPLGDYAA
                     KEMKLKRVATITEDFAYGYEQTGGFQRVFEDNGGRIVKKLWTPIATPDYTPYLAQISD
                     VDGVWNGFAGSNPLKFNQQYAELGLKAKYPVMGGWTAYDDALLKSFGDEAIGAISAAW
                     YSSDLDTPTNKRFVEEMVKETGGVPGGYAAGTYINGMCIEAALAKTGGKTDDKQALIK
                     ALRAVSLTDTPRGAF"
     gene            1358..>2411
                     /locus_tag="VN518_04725"
     CDS             1358..>2411
                     /locus_tag="VN518_04725"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HXE03911.1"
                     /translation="MQLGYFTMPLHPPERDYTLTLREDREAIILADKLGFIEAFVGEH
                     VTDRAETITSCLTFIASLISETRRIKLGSGTVNLPNGHPAAIAAEVAMLDHLLEGRFL
                     FGISPGGLRSDAEVFGNLDKDRTAMFVEAIDMVLKIWSSEPPYRLAGKFWTVTTEKTL
                     DKAIGQGVIVKPYQQPHPPIVVTVVAPFSKGVVAAAERGWTPISANFLQPPWVASHWP
                     MYEQGCKNAGRTARREDWRVAKSVFVCDDEAMAQRYAKGEGSPYRFYYRNLMKKLIGN
                     GRPELFKHDRSLPDEAITLDYVVDSLVICGTVNSVVDQLLAFRERIGDFGTLLYAGHD
                     WVDPGLSKRSMELMASE"
BASE COUNT          409 a          786 c          762 g          454 t
ORIGIN      
        1 gaaagcgccg cgcggcgtgt cggtcagcga aacggcgcgc agcgccttga tgagcgcctg
       61 cttgtcgtcg gtcttgccgc cggtcttggc aagtgccgcc tcgatgcaca tgccgttgat
      121 gtaggtgccc gcggcatagc cgcccggcac gccgcccgtc tccttgacca tctcctcgac
      181 gaagcgcttg ttggtgggcg tgtcgaggtc gctcgaatac cacgcggcgg agatggcgcc
      241 gatcgcctcg tcgccgaagc tcttcaggag cgcgtcgtca taggccgtcc atccgcccat
      301 gaccggatat ttcgccttga gcccgagctc ggcatattgc tggttgaact tgagcggatt
      361 ggacccggca aagccgttcc acacgccatc gacatcgctg atctgcgcga gatagggcgt
      421 gtagtcgggc gtggcaatcg gcgtccatag cttcttgacg atgcggccgc cattgtcttc
      481 gaagacgcgc tggaacccgc cggtctgctc gtagccatag gcgaaatcct cggtgatcgt
      541 cgccacgcgc ttgagcttca tctccttggc cgcgtaatcg ccgagcggct ggttcacctg
      601 cgacgaggtc gccgaggcgc gcacgaaata ggggttgggc ttgcgctgcg tcatgtcctc
      661 ggcggcggcg atgctgagca tcggcacttt cgcctgctcg acataggagc tgatcgccag
      721 cagctcaaag gcggccagcg ggccgacaat gaaatcgaca tggtcgcgct cgaccagctc
      781 ctgcgccttg gtcttggcgc cggcgggatt gccgccggta tcgccgacga cgagttcgac
      841 cttgcggccg gcgagcgtgt tgttcttctc cttgagatag gcggtgatgc cctgctccat
      901 ctggatgccg ccttgcgcca gcggcccggt cttgaccgtc aaaaggccga tgcgggccgg
      961 cgcggcctgc gcaatggcgg gcgcccacgg caaggtggtg agagccgtgc cccacaggaa
     1021 cgtgcgtcga ttgagcttcc acgccatcaa gaacctccct tttcgcggcc cccgcggggc
     1081 tcgtgttttg gcgttcggcc ggtcgccgaa gatcgccgct gctgcggcaa tatcatgctg
     1141 aaccgtccgg cgcgatcaag atgccagcga tgatcacgga gcgccgccat cccgtccagc
     1201 aaaaaacgcc gcgacgccaa cttagctcct tgtcgccgct ggctatttgc cgcagggaat
     1261 tccagtgacc cccgggcttg ccttttgcga agattttatc agatgataag aagctcggcc
     1321 agatcgatgg cgccagggag tatgcccgga gggacagatg cagctcggct acttcacgat
     1381 gccgctccat ccgccggagc gtgactacac gctgacgctg cgtgaggatc gcgaggcgat
     1441 catccttgct gacaagctcg gcttcatcga ggctttcgtc ggcgagcacg tcaccgaccg
     1501 cgccgagacc atcacctcct gcctcacctt catcgcgagc ctcatctcgg agacgcgccg
     1561 gatcaagctc ggcagcggca cggtcaatct gcccaacggc catcccgccg cgatcgccgc
     1621 ggaagtcgcg atgctcgacc atctgctcga gggccgtttc ctcttcggca tcagcccggg
     1681 cggattgcgc tccgacgccg aggtcttcgg caatctcgac aaggaccgca ccgcgatgtt
     1741 cgtcgaggcg atcgacatgg tgctgaagat ctggtcctcg gagccgccct atcgcctcgc
     1801 aggcaagttc tggacggtga cgacggaaaa aaccttggac aaggcgatcg gccagggcgt
     1861 catcgtcaag ccctatcagc agccgcaccc gccgatcgtc gtcactgtcg tggcgccatt
     1921 ctccaagggc gtcgtggcgg cggccgagcg cggctggacg ccgatctcgg ccaatttcct
     1981 gcagccgccc tgggtcgcct cgcattggcc gatgtatgag caaggctgca agaatgccgg
     2041 gcggacggca cggcgcgagg attggcgcgt cgccaagagc gtcttcgtct gcgacgatga
     2101 ggccatggcc cagcgctacg ccaagggcga aggcagccct tatcgcttct actatcgcaa
     2161 cctcatgaag aagctcatcg gcaatggccg gcccgagctc ttcaagcatg accgcagcct
     2221 tccggatgag gcaatcacgc ttgattacgt ggtcgacagc ctcgtcatct gcggcaccgt
     2281 caacagcgtc gtcgaccagc tcctcgcgtt tcgcgagcgc atcggcgatt tcgggacgct
     2341 tctctatgcc ggccacgact gggtcgatcc gggtttgagc aagcgctcga tggagctgat
     2401 ggcgagcgag g
//
LOCUS       DATNAE010000207         3075 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_502520, whole genome shotgun sequence.
ACCESSION   DATNAE010000207 DATNAE010000000
VERSION     DATNAE010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502520"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="VN518_04730"
     CDS             complement(<1..444)
                     /locus_tag="VN518_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HXE03912.1"
                     /translation="MFPKPRQDLRPNTAEFERLPFVKATGFREYDARWRYPEEINLLG
                     LEAVGLALADLMRARGVPTRVVVGHDYRSYSASIKAALINGLLAGGAEVHDIGLALSP
                     MAYFAQFDLGIEGVTMVTASHNDNGWTGIKMGVERPLTFAPDDMTA"
     gene            complement(540..1241)
                     /locus_tag="VN518_04735"
     CDS             complement(540..1241)
                     /locus_tag="VN518_04735"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HXE03913.1"
                     /translation="MSAWIAIFVLALAAMLLVSNSSGTIANLDSDTFGYVALGTALLV
                     FIGGGVLVRYRGQAGAMFRDAVTWLALGLALVTLYSYREELLPVAGRLAGELLPGSAM
                     VVEQSPGGPVEIKIRKRLDGHFTAKAEVNGISIPMIVDTGASTIVLRPEDAKKAGIDV
                     SKLSFTVPVMTANGRTVAARVRLDSVAIGPLQREDVDALVAEPKALTESLLGMSFLGR
                     LRSYEFSGDFLTLRG"
     gene            complement(1246..1422)
                     /locus_tag="VN518_04740"
     CDS             complement(1246..1422)
                     /locus_tag="VN518_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HXE03914.1"
                     /translation="METPCIDVCEMDACSGLCIGCGRTLDEIAGWADMSPEERRAIMA
                     VLPARKAPNETVKG"
     gene            complement(1503..>3075)
                     /locus_tag="VN518_04745"
     CDS             complement(1503..>3075)
                     /locus_tag="VN518_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HXE03915.1"
                     /translation="FAGFTGMTIGVSAEARTAASAAMNAKRSPSRGHRQAHDRLTPLS
                     GFGSELDYELLLLFAKNELSAAVTTPLLAVIVAVGAMFWAPPAQLLLWLATVFFAKGL
                     LISLCRQFIKTPREEVKPQAWRAKMMAAEFLYGLSWAAVAFVSSGAAGEAAHTFVFAA
                     LLIVISMRMLFASPVMPIVFAGTLPMAAAIVVRFALLDNPFYWAMAAMAIGVYVYFIW
                     LMNGTNATITAMIGLRAEKDALIAELEQSKSISDEARLRAEEASQAKSRFLATISHEL
                     RTPLNAILGFSEIMRSEILGAHDNPVYKEYANDIHQSGQHLLNLINEILDISRIESGR
                     YELHEAPVALATVVEDCQRLLRLRAETKALRIIEQFEPELPQLWADERAMRQICLNLL
                     SNAIKFTPPGGTITLAIGETESRGQFLSVKDTGPGIPENEIPRVLKSFGQGSLAHQTA
                     EGGTGLGLPITKGLAELHDGTFELKSRLRQGTEVKVSFPRKRVMEPVPRIVEPARWLK
                     DRQNSSAAMGAAAKT"
BASE COUNT          569 a          961 c         1028 g          517 t
ORIGIN      
        1 cgcggtcatg tcgtcgggcg cgaaggtcag cggccgctcg acgcccatct tgatgccggt
       61 ccagccattg tcgttgtggc tcgccgtcac catggtgacg ccttcgatgc caagatcgaa
      121 ctgcgcgaaa taggccatag gcgacagcgc caagccaatg tcgtgaacct cggcgccgcc
      181 ggcgagcagg ccgttgatca gcgctgcctt gatcgaggcc gaataggagc ggtagtcgtg
      241 gccgaccacg acacgagtgg gcacgccgcg cgcgcgcatc agatcggcga gggcgagccc
      301 cacggcttca agcccgagga gattgatctc ttcgggatag cgccagcggg cgtcgtattc
      361 gcgaaatccg gtcgccttga cgaagggaag gcgctcgaat tcggccgtgt tgggtcggag
      421 atcctggcgc ggcttgggaa acatgctgcc ccctgatagg cgattttcga cgtttcacca
      481 acagcgctga ggagggcgag gttcccgctg gcgcaggccg atcaactcag tccttaacgt
      541 cagccgcgca gcgtcaggaa atcccccgag aattcatagg atcgcagccg cccgaggaag
      601 ctcatgccga gcaggctctc ggtcaatgcc ttgggctcgg ccacgagcgc atcgacatct
      661 tcgcgctgaa gcgggccgat cgcgacgctg tccaggcgca cccgtgccgc gacggtgcgg
      721 ccgtttgcgg tcatcaccgg cacggtgaag ctgagcttgc tcacgtcgat gccggccttc
      781 ttcgcatcct ccgggcgcag cacgatggtc gaggcgccgg tatcgacgat catggggatg
      841 gaaatgccat tgacctcggc cttggcggtg aaatggccgt cgaggcgctt cctgatcttg
      901 atctcgaccg gcccgccggg gctttgctcg acgaccatgg cgctgccagg aagaagctcg
      961 ccggcgaggc gcccggcaac cggcagaagc tcctcgcgat aggaatagag cgtgacgagc
     1021 gcgagcccga gcgcaagcca agtgaccgcg tcgcggaaca tggcgccggc ctgcccgcga
     1081 tagcgcacca gcacgccgcc gccgatgaac acgagaagcg cagttccgag cgccacatag
     1141 ccgaaggtgt cgctgtcgag gttggcgatg gtgccggagg agttgctcac gagcagcatg
     1201 gctgcgaggg ccaggacaaa aatggcaatc cacgccgaca attctttagc ctttcactgt
     1261 ctcgttgggc gccttccggg ccggcagcac cgccatgatg gcgcgccgct cctcggggct
     1321 catgtccgcc caccccgcga tctcatcgag agtacgcccg cagccaatgc acagacctga
     1381 gcaagcgtcc atctcgcaga cgtcgatgca gggagtttcc aagggaggct tcggtccggt
     1441 ggtcaaccaa gcctatgtag ggggtgaaca gccgccggac aatcgccttt ccgccgcggg
     1501 gatcaggtct tggcggcggc tcccatcgca gcggaagagt tctgccggtc cttgagccat
     1561 cgcgcgggct cgacgatccg agggaccggc tccatgacgc gcttgcgcgg aaagctcact
     1621 ttgacctcgg tgccctggcg aagcctgctc ttcagctcga acgtgccgtc atgcagctcg
     1681 gccagtccct tggtgatggg aagcccaagc ccggtgccgc cttccgccgt ctgatgggca
     1741 agagacccct ggccgaagct cttcagcacg cgggggatct cgttctcggg aatgcccggc
     1801 ccggtatcct tgacgctcag gaactgcccg cgggactcgg tctcgccgat ggcgagcgtg
     1861 atcgttccgc ctggcggggt gaacttgatc gcattcgaca ggaggttgag gcagatctgg
     1921 cgcatggccc gctcatcggc ccagagctga ggaagctctg gctcgaactg ctcgataatg
     1981 cgaagggcct tggtctcggc gcgtaagcgc agcagacgct ggcagtcctc gaccacggtg
     2041 gcgagcgcga ccggcgcctc gtgcagctcg tagcggcccg actcgatgcg ggaaatatcg
     2101 aggatctcgt tgatcagatt gagcaggtgc tggccgctct ggtggatgtc gttggcgtat
     2161 tccttgtaaa ccgggttgtc gtgcgccccc aagatctccg agcgcatgat ctccgagaag
     2221 ccgaggatgg cgttgagcgg cgtgcgcagc tcatggctga tggtggcgag gaagcgggac
     2281 ttcgcctggc ttgcttcctc ggcgcgcagc cgggcctcgt cggagatcga tttcgactgc
     2341 tcgagctcgg cgatgagggc gtccttctcg gcgcgaagcc cgatcatggc ggtgatcgtg
     2401 gcgttggtgc cgttcatcag ccagatgaaa tagacataga cgccgatggc catcgctgcc
     2461 atcgcccagt agaacgggtt gtcgagcagc gcgaagcgga ccacgatcgc cgccgccatc
     2521 ggcagggtgc cggcgaagac gatgggcatg accggcgaag cgaacagcat gcgcatcgag
     2581 atgacgatga gaagagcggc aaagacgaag gtatgcgcgg cctctccggc ggcgcccgag
     2641 ctgacgaagg caaccgccgc ccaggacaat ccgtagagaa actcggctgc catcatcttg
     2701 gcgcgccagg cctgcggctt gacctcctcc cgcggcgtct tgatgaattg ccggcaaagg
     2761 ctgatcagca ggcccttggc gaagaaaacc gtggcgagcc agagcaacaa ttgtgctggg
     2821 ggcgcccaga acatcgcacc cacggccacg atcaccgcga gcagcggcgt ggtcaccgcc
     2881 gccgacagct cgttcttggc gaacaaaagc aggagctcgt aatcgagctc cgagccgaaa
     2941 ccggagagcg gggtgagcct gtcgtgagcc tgccggtgcc cgcgggaagg agaccgtttg
     3001 gcgttcattg ccgccgaagc ggccgtacga gcctcggctg aaacgcctat cgtcatgcct
     3061 gtaaagcccg caaaa
//
LOCUS       DATNAE010000208        13060 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3808994, whole genome shotgun sequence.
ACCESSION   DATNAE010000208 DATNAE010000000
VERSION     DATNAE010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 13060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13060
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3808994"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..419
                     /gene="trxA"
                     /locus_tag="VN518_04750"
     CDS             84..419
                     /gene="trxA"
                     /locus_tag="VN518_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HXE03916.1"
                     /translation="MATATVSDDTFEADVLKSSTPVVVDFWAEWCGPCRQIAPALEEI
                     AKELHGKVKIVKLNIDENHRTPSRYNVRAIPTLMIFKDGQLAATQTGALPKGRLADWI
                     SGTIGVAAA"
     gene            complement(416..1771)
                     /locus_tag="VN518_04755"
     CDS             complement(416..1771)
                     /locus_tag="VN518_04755"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HXE03917.1"
                     /translation="MTNLPHSDEGRIPLPSVSGSVTLLERLKALHPQSIDLSLGRIER
                     LLAALDRPERRLPPVVHVAGTNGKGSLIAFVRAIAEALGKRVHVYTSPHLVNFHERIL
                     LAGPDGGAPITEDRLVDCLARAEAANSGELITLFEITTAAAFLAFAESQADLLLLETG
                     LGGRLDATNVVEKPLATSITPISIDHVSFLGNTLAAIAAEKAGILKPGVPCIVGRQEA
                     EALRVIEARAEEIGAPLHVFGRDFDVFEQHHRLIFQTASRLIDLPLPRLNGRHQIDNA
                     GAAIATASVAFGDALTIDSLERGLTHAEWPARLERLGEGALHQYVGEGTEIWLDGGHN
                     AAGGQAIAHALAEFDERVPRAVHLIWGMMETKDAHAFIAPFKGLVERVYTVPIPEEAN
                     AFSAEALAEIAASEGFDVTPASGVAHALMQSKAALGRPGRVLICGSLYLAGHVLKLHG
                     S"
     gene            complement(1783..2823)
                     /gene="accD"
                     /locus_tag="VN518_04760"
     CDS             complement(1783..2823)
                     /gene="accD"
                     /locus_tag="VN518_04760"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HXE03918.1"
                     /translation="MNWINNVVRPKIRSILRKKEMPDNLWVKCPETGQMIYYKDLEAN
                     QFVVPGSNYHMRVSAGARLKGLFDGGEYETIALPEVPIDPLKFRDERRYTDRIKDARS
                     KTGLADALLVGAGKLQGDKIVAAVQDFDFMGGSLGTAAGEAVISGMRTAVKRHSPFIL
                     FAASGGARMQEGILSLMQMPRTTVAVQELREAKLPYVVVLTHPTTGGVTASYAMLGDI
                     HIAEPGALIGFAGPRVIEQTIREQLPNGFQRAEYLLDHGMVDMVVHRHQLRDTLGRIC
                     RLLTHTRPGRDIAIPDHLDGMNSHPAPVTTLARLGGNGEIVDIEVEEDDDDDHVNEPF
                     GTPAEPRPPRNV"
     gene            complement(2875..3708)
                     /gene="trpA"
                     /locus_tag="VN518_04765"
     CDS             complement(2875..3708)
                     /gene="trpA"
                     /locus_tag="VN518_04765"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HXE03919.1"
                     /translation="MSRIEPRFAALKREGRAGLVTFITAGDPDYATSLKLLKALPQAG
                     ADLIELGMPFSDPMADGPAIQASSQRALAAGQTMVKTLAMVKSFREKDETTPIVLMGY
                     YNPIYIYGVRFLKDAVAAGVDGLIVVDMPPEEDAELRPPASEAGLNFIRLATPTTDAK
                     RLPAVLANTSGFVYYVSIAGITGTAAPNLADVGAHVARIKTATKLPIAVGFGVKTKEQ
                     VSAIAKLAEGVVVGSALVSAIAQSLDAEGKATPETGPKALALVEHLASALRTPATAAV
                     L"
     gene            complement(3705..4967)
                     /gene="trpB"
                     /locus_tag="VN518_04770"
     CDS             complement(3705..4967)
                     /gene="trpB"
                     /locus_tag="VN518_04770"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HXE03920.1"
                     /translation="MSAPLKQAPPNSYRTGPDERGHFGLYGGRFVAETLMPLILDLEQ
                     AYNEAKADPSFQAEMDCHLATYVGRPSPLYFAERLTQHCGGAKIYFKREELNHTGAHK
                     VNNVLGQIMLAMRMGKTRVIAETGAGMHGVATATLCAKFGLPCIVYMGSVDVERQKPN
                     VLRMKILGAEVRAVESGSKTLKDAMNEALRDWVANVHDTFYCIGTVAGPHPYPAMVRD
                     FQSIIGRETREQMLAAENRFPDSLVACIGGGSNAMGLFHPFLDERGIEIYGVEAAGLG
                     LNSGKHAASIAGGRPGVLHGNRTYLLMDGDGQIEEAHSISAGLDYPGIGPEHAWLNDM
                     ARVKFLSATDAEAVAAFQLCSKLEGIIPALEPAHALARVLDLAPQKPKDHLMVVNVSG
                     RGDKDLDSVASYLSAKTRGASSRPGSPA"
     gene            complement(4964..5632)
                     /locus_tag="VN518_04775"
     CDS             complement(4964..5632)
                     /locus_tag="VN518_04775"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HXE03921.1"
                     /translation="MPVKVKICGVRTREIVDTAVDAGADYVGLVFFPNSPRYVAPDAA
                     RPLAEAAAGRIETVAVLVDPDDALIDDILATVRPGLLQLHGSETPDRVAAVKARSGLR
                     VIKTIGVATADDVAKAAAYRGIADMILFDAKAPAGSNLPGGHGLAFDWRSLDGPSVER
                     PFALSGGLDPDNVWEALAVTGASMVDVSSGAERAPGEKDPDLVRRFIQAAKAANPLKK
                     AVGS"
     gene            complement(5758..6099)
                     /locus_tag="VN518_04780"
     CDS             complement(5758..6099)
                     /locus_tag="VN518_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018065552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HXE03922.1"
                     /translation="MRKLVAALILVPIGVLLVALAVVNRKPVMLGLNPFDANAGLGIE
                     APLFLFLLGAFALGLVIGGFATWLGQGKWRKTARVEAREASTWRRQADRLEKELEGLD
                     ARPRRARVAAD"
     gene            complement(6126..6419)
                     /gene="ihfB"
                     /locus_tag="VN518_04785"
     CDS             complement(6126..6419)
                     /gene="ihfB"
                     /locus_tag="VN518_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HXE03923.1"
                     /translation="MIKSELIQRVAAANPHLFQRDVERIINIVLDEITGALARGDRVE
                     LRGFGAFTVKHRAARQGRNPRTGDTVFVEDKFVPFFKTGKELRERLNREDGAA"
     gene            complement(6460..7419)
                     /gene="sppA"
                     /locus_tag="VN518_04790"
     CDS             complement(6460..7419)
                     /gene="sppA"
                     /locus_tag="VN518_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HXE03924.1"
                     /translation="MIEAETVIERRRLRRRLTAWRLAAVVLAVFVIALIMAGDQRLAG
                     TGSFLPHIARVSVSGFITDDQKMLELLDKVGKSSQVRAVILDINSPGGTTTGGEAMYD
                     AIRRLAEKKPVVAVCGTLATSAAYIVALATDRIFVYGNTITGSVGVIFQWADVSELMR
                     TLGVKVEEVKSGPLKAVPNPFEPTDEKGRALAAEMVQEAKVWFVDLVGKRRNLEPAAV
                     PGLTDGRVYSGRQALALKLVDEIGDERAAKRWLQKERDVTPGLSVVEWKPKSESGGIW
                     GWLFGSIASSFGIAADNFVSLFGQVSEGLRLDGLVSLWHPAGS"
     gene            complement(7487..9292)
                     /gene="rpsA"
                     /locus_tag="VN518_04795"
     CDS             complement(7487..9292)
                     /gene="rpsA"
                     /locus_tag="VN518_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HXE03925.1"
                     /translation="MNEPRASISHPSHDEFAALLEESLTTTALQEGNVVKGTIVGIEK
                     DVAVIDVGLKTEGRIPLKEFGISGRAANLSVGDEVDVYLERVENAMGEAVLSREKARR
                     EESWTRLEEKYKAGERVEGVIFNRVKGGFTVDLDGAVAFLPGSQVDIRPIRDVGPLMN
                     QPQPFQILKMDRRRGNIVVSRRSVLEETRAEKRSEIVARLEEGQVIEGVVKNITDYGA
                     FIDLGGIDGLLHVTDMAWRRVAHPSEVVNVGDSVKVQIIRINPETQRISLGIKQLEAD
                     PWEGIAAKYPVGSRLKGTVTNITDYGAFVELEPGVEGLIHVSEMSWTKKNIHPGKIIS
                     TSQEVEVQVLEVDPEKRRISLGLKQTQENPWVAFAANHPPGTNIEGPIRNITEFGLFI
                     GLDGGIDGMVHLSDLDWSKSGEDALKDYNKGDNVKAVVLEADSEKERISLGVKQLTPD
                     PFASGAPSAKLKKGMRVTCEVVAVKDSGLEVKLVDHDLTSFVRRSDLSRDRSEQRPDR
                     FGVGEKFDAVVTQVDAKARKVGVSIKALEIAEEKEAVAQYGSTDSGASLGDILGAALS
                     KAKEEDDDEAAEKPKRKSKAKSESAKTEDSAEAEE"
     gene            complement(9456..10097)
                     /locus_tag="VN518_04800"
     CDS             complement(9456..10097)
                     /locus_tag="VN518_04800"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0036431 - dCMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006220 - pyrimidine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="d(CMP) kinase"
                     /protein_id="HXE03926.1"
                     /translation="MIVAIDGPAASGKGTIAKRVAAHYGLAYLDTGTLYRAVARDTLA
                     RSEDPADAIEALKSAKALDPFTLGDPGLRKPGLAEAASLVAKHPEVREELLAYQRSFA
                     RAKSGAVLDGRDIGTVIFPDADVKLFVTATPEERASRRYLELKQAGIEVSEAEVQCDI
                     RRRDERDKHRAAAPLRRAPDAHLLDTTNLDIDAAFRAAIDLIDAALARAGYAG"
     gene            complement(10094..11410)
                     /gene="aroA"
                     /locus_tag="VN518_04805"
     CDS             complement(10094..11410)
                     /gene="aroA"
                     /locus_tag="VN518_04805"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HXE03927.1"
                     /translation="MSQPLSAVPTRALAGTIRVPGDKSISHRALILGALATGRTRITG
                     LLESEDVLATARAVVALGAWIRHVNGSVEVTGQGVGGLQAPIDDLDFGNSGTGSRLML
                     GVVAGHDMEARFTGDASLCRRPMARVLKPLQRMGLAVGDDTRTTLPLTITGTPDLVPI
                     VYQSPIASAQVKSAVLLAGLHAPGRTTVIEPIPTRDHTERMLTYFGAEVSVKDEDDGR
                     RAVTVRGDAELSGASIAIPGDPSSAAFAIAAALICEGSDIVLNGVLMNPTRAGFIETV
                     REMGGDVDLLDLRLEGGEPAADLRVRAGALSGVDVPPGRAPSMIDEYPVLAVLAAFAE
                     GETRLRGLAELRVKESDRLAATAAGLVACGVETAIEGDNLIVMGKGSVPGGGVVETHM
                     DHRIAMAFLTLGLGAGQPVTVDDIGMIATSFPAFVPLMTKLGAQFR"
     gene            11512..11916
                     /locus_tag="VN518_04810"
     CDS             11512..11916
                     /locus_tag="VN518_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014891601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02300 family protein"
                     /protein_id="HXE03928.1"
                     /translation="MSKPARGIKRVCQSCGARFYDLGRTPIVCPSCQAVYQVTQPTSR
                     RGERAPVPEVREKVEPEVEAVALEPETISLEEVEEPGEEAAIEDEEIVDLGEDEAEIP
                     AAGEDDTFLEEPEGDEEADVSGIVGGPEKEEG"
     gene            12077..12152
                     /locus_tag="VN518_04815"
     tRNA            12077..12152
                     /locus_tag="VN518_04815"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12110..12112,aa:Ala,seq:ggc)
     gene            complement(12706..>13060)
                     /locus_tag="VN518_04820"
     CDS             complement(12706..>13060)
                     /locus_tag="VN518_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03929.1"
                     /translation="RLRFRLRGARAPDRCFVGLDDGDGARGSAGLPASKVGETFRQTM
                     RCGETNEGVASMTNANTESLVVEFIKEVANMREREATDPEIQSMLEFVEQLTRWRGDV
                     DREPLINALREAARR"
BASE COUNT         2319 a         4113 c         4313 g         2315 t
ORIGIN      
        1 taagggggtg cgcacctagg ttcggacgtg acgacaagac cggccagttc aggggtcgaa
       61 tgccggcgca tcaggagaag caaatggcaa ctgcaactgt ttccgacgac accttcgagg
      121 ccgatgtatt gaagtcatcc acgccggtgg tggtggactt ctgggccgaa tggtgcggcc
      181 cctgccggca aattgcgccg gcgctcgagg agatagccaa ggagctccac ggcaaggtca
      241 agatcgtcaa gctcaacatc gatgagaatc atcggacgcc gtcgcgctac aatgtgcgcg
      301 cgatcccgac gctaatgatc ttcaaggacg gccagctcgc ggcaactcag acaggggcgc
      361 tgccgaaggg caggcttgcc gactggatca gcgggacgat cggcgtcgcc gcagcctagc
      421 ttccatgcag cttgagcacg tggcccgcta gatagagcga gccgcagatc agcacgcgtc
      481 cggggcggcc tagagccgcc ttggactgca tgagcgcgtg cgccacaccg ctcgccggcg
      541 tcacgtcgaa gccttcgctc gcggcgatct ccgccaaggc ttctgccgag aaggcgttcg
      601 cctcctctgg gatcggcacg gtgtagacgc gctcgaccag gcccttgaat ggagcgatga
      661 aggcgtgcgc gtccttcgtc tccatcatcc cccagatgag atgaacggcg cgcggcacgc
      721 gctcgtcgaa ctcagcgagc gcatgggcaa tggcttgacc gcctgctgca ttgtgcccgc
      781 catcgagcca aatctcggtc ccctcgccca catattggtg gagcgcacct tcgccgaggc
      841 gctcaagccg cgccggccat tcggcatggg tcagtcccct ctcaagcgag tcgatggtga
      901 gcgcatcgcc gaaagcgacg ctcgcggtgg cgatagcagc gccggcattg tcgatctggt
      961 gccgcccgtt gaggcgcggc agaggaaggt cgatcagccg cgacgcggtc tggaagatca
     1021 atctgtggtg ctgctcgaag acgtcgaagt cgcgcccgaa cacatggagc ggcgcgccga
     1081 tctcttccgc ccgcgcttcg atcacccgaa gcgcttcggc ttcctggcgg ccgacaatgc
     1141 agggcacgcc aggcttgaga atgccggcct tctcggcggc aatcgccgca agcgtattgc
     1201 ccagaaatga aacgtgatcg atcgatatgg gggtgatgga ggtggcaagc ggcttctcga
     1261 cgacgttggt ggcatcgagc cggccgccaa ggccggtttc cagcagcaac aaatccgctt
     1321 gtgactcggc aaaggcgagg aaggccgccg cggtggtgat ctcgaagagc gtgatcagct
     1381 cgccggaatt ggcggcctcg gcgcgggcga ggcagtcgac gagcctgtcc tcggtgatcg
     1441 gcgcgccgcc atcaggcccg gcaagcagaa tgcgctcgtg gaagttgacg aggtgcggag
     1501 aggtgtagac gtgcacgcgc ttgccgagcg cctcggcgat ggcgcggacg aaggcgatca
     1561 gcgagccctt gccgttggtt cccgcgacat ggaccacggg cggcaggcgg cgctcgggac
     1621 ggtcaagggc cgcgagcaga cgctcgatgc gcccgagcga caggtcgatc gattgcgggt
     1681 gcagcgcttt gaggcgctcg agcagcgtga cgcttcccga gaccgagggc agtgggatgc
     1741 ggccctcgtc gctgtggggc aaattggtca aggagattgg ccttagacgt ttcgcggcgg
     1801 acgcggttcg gcaggggtgc cgaagggctc gttcacatga tcgtcgtcgt cgtcctcctc
     1861 gacctcgata tccacgatct cgccgttgcc gccgagcctg gccagcgtcg taaccggcgc
     1921 cggatgcgag ttcatgccgt cgaggtggtc gggaatggcg atgtctcgcc cgggcctcgt
     1981 gtgggtgagc agacggcaga tgcggccgag cgtgtcgcgc agctgatggc gatgcaccac
     2041 catgtcgacc atgccgtggt caaggagata ttcggcgcgc tggaaaccgt tgggcagctg
     2101 ctcgcgaatg gtctgctcga tgacgcgagg accggcgaaa ccgatcagcg cgccgggctc
     2161 ggcaatgtgg atgtcgccga gcatggcgta ggaggcggtg acgccgccgg tggtcggatg
     2221 ggtcagcacc acgacataag gaagcttcgc ctcgcgcagc tcctgcaccg ccacggtggt
     2281 gcgcggcatc tgcatcagcg aaaggatgcc ttcctgcatg cgcgcgccgc cagaagcggc
     2341 aaacaaaatg aacgggctat ggcgcttcac cgccgtgcgc atgccggaaa tcaccgcttc
     2401 gccggcggcg gtgccgagcg agccgcccat gaaatcgaaa tcctgcacgg cggcgacgat
     2461 cttgtcgcct tgaagcttgc cggcgccgac aagaagcgcg tcggcaaggc cggtcttgct
     2521 gcgggcgtcc ttgatgcggt cggtgtagcg ccgctcgtcg cggaatttga gcgggtcgat
     2581 cggcacctcg ggaagagcga tggtctcgta ttcgccgccg tcgaacaggc ctttcagccg
     2641 cgcccccgcc gaaacgcgca tgtggtaatt ggagcccgga accacgaact ggttggcttc
     2701 gagatccttg taatagatca tctgccccgt ttcggggcat ttgacccaca gattgtccgg
     2761 catctccttc ttgcgaagga tgctgcgaat cttgggcctg acgacgttgt tgatccagtt
     2821 cacgggacta ccaaaagttc aaaagcaaca ctcaacctaa actcgaaatc atgcctaaag
     2881 cacggcggcc gtcgccgggg tacgaagcgc cgaggcgaga tgctccacca gagccagagc
     2941 tttggggccg gtttcgggcg tcgccttgcc ttcggcatcg aggctttggg cgatcgccga
     3001 cacaagtgcc gagccgacca ctacgccctc ggcgagcttg gcgatggcgc ttacctgctc
     3061 cttggtcttc acgccgaagc cgacggcaat ggggagcttg gtcgcagtct tgatgcgggc
     3121 cacatgggcg ccgacatcgg cgagatttgg cgcagccgtg cccgtaattc cggcaatcga
     3181 cacgtaatag acgaaccccg aagtattggc gagcacagcc ggaaggcgct tggcgtcggt
     3241 cgttggtgta gccaggcgaa tgaagttcag tcccgcctcg cttgccggcg ggcgaagctc
     3301 ggcatcttcc tctggcggca tatcgaccac gatcaagcca tcaactccgg cggcgaccgc
     3361 gtccttcagg aaccgaacgc cgtaaatata tatggggttg taatagccca ttaacacgat
     3421 gggggtggtt tcgtcctttt ccctgaagga cttaaccatt gccaaggtct tcaccatggt
     3481 ctggccagcg gcaagcgcac gctgcgagga ggcctggatc gccggcccgt ccgccatcgg
     3541 gtctgagaag ggcatgccaa gctcgatgag atcggcgccg gcttgcggca gcgctttcag
     3601 cagcttgagg gaggtggcgt aatcgggatc gccggcggtg atgaaggtca cgagcccggc
     3661 gcggccttcg cgcttcagcg cggcgaagcg tggttcgatc cgactcatgc tggcgatcct
     3721 ggccgcgagc tcgctcctcg cgtcttggcg ctcagatagc tcgccacgga gtcgaggtcc
     3781 ttgtcgccgc ggccggaaac attgaccacc atcaaatgat ccttcggctt ctgcggcgcg
     3841 aggtcgagca cgcgggcgag cgcatgcgcg ggctccagcg ccgggatgat gccttcgagc
     3901 ttgctgcaaa gctggaacgc ggccaccgcc tcggcgtcgg tcgccgagag gaatttcacg
     3961 cgggccatgt cattgagcca ggcatgctcg ggtccgatgc ccggataatc gaggccggcc
     4021 gagatcgaat gcgcttcctc gatctgcccg tcgccgtcca tcagcagata ggtgcggttg
     4081 ccgtgcagca cgcctggcct gccgccggca atcgaggccg cgtgcttgcc ggagttgagc
     4141 ccgaggccgg cagcttccac gccgtagatt tcgatcccgc gctcgtcgag gaagggatgg
     4201 aacaggccca tggcattgga gccgccgccg atgcaggcga ccagcgaatc gggaaaacgg
     4261 ttttccgccg caagcatctg ctcgcgcgtc tcgcggccga tgatcgactg gaaatcgcgg
     4321 accatcgccg ggtaagggtg cggccccgcc accgtgccga tgcaatagaa cgtgtcgtgc
     4381 acgttggcga cccaatcgcg cagcgcctcg ttcatcgcat ccttgagcgt cttggaaccc
     4441 gattcgacgg cgcgcacctc ggcgccgagg atcttcatcc tgagcacgtt cggcttctgc
     4501 cgctcgacgt cgacggagcc catgtagacg atgcagggga gcccgaactt cgcgcacaag
     4561 gtcgcggtgg cgacgccatg catgccggcg ccggtctcgg cgatgacgcg cgtcttgccc
     4621 atgcgcatgg ccagcatgat ctggccgagc acattgttca ccttgtgcgc gccggtgtgg
     4681 ttgagctcct cgcgcttgaa atagatcttg gcgccgccgc agtgctgcgt gagccgctcg
     4741 gcaaaataaa gcggcgacgg cctgccgaca taggtcgcga gatggcagtc catctcggcc
     4801 tggaaggacg gatcggcctt cgcctcgttg taggcctgct ccagatcgag gatcagcggc
     4861 atcagggtct cggcgacgaa gcggccgcca tagaggccga aatggccgcg ctcgtcgggc
     4921 ccggtgcggt aggagttggg cggagcctgc ttgaggggag cgctcatgag ccaacagcct
     4981 tcttgagcgg gtttgccgcc ttggcggcct ggatgaagcg gcgcacgagg tcagggtcct
     5041 tctcgcctgg agcccgctcg gcgccggagg atacatcgac catcgacgct ccggtgacgg
     5101 cgagcgcctc ccagacattg tcagggtcga gcccgccaga cagcgcaaag ggccgctcga
     5161 ccgacggacc gtcgagagag cgccagtcga acgcgagacc atgaccgccg ggaaggttgg
     5221 agccggcagg cgctttggcg tcgaacagga tcatatcggc aatgccccgg taggcggcgg
     5281 ctttggccac atcgtcggcg gtggcgacgc caatggtctt gatcacgcgc aagcctgagc
     5341 gcgccttgac cgccgccacc cggtcggggg tctcgctgcc gtgcagctga agcaggccgg
     5401 ggcgcaccgt ggcgagaatg tcatcgatca gcgcatcgtc cggatcgacg agcaccgcga
     5461 ccgtctcgat gcggcctgcg gcagcttcgg cgagcggccg cgcggcgtcg ggcgcgacat
     5521 aacgggggct gttcgggaag aacacgaggc cgacatagtc ggcgccggca tcgactgccg
     5581 tgtccacgat ctctcgcgtg cgaacgccgc aaattttgac cttcaccggc acctggcaga
     5641 ctttccatgg ctgggggtgg agggctctca acaagcgccc gggcggcgcc accctatgca
     5701 attcgggggc cgaagtctat cgcagtgcag caaaccgccc ccaggcaggg aagtccctca
     5761 gtcggcggcg acgcgggcgc gccggggacg ggcgtcgagg ccttccagct ccttctccag
     5821 gcggtcggcc tggcggcgcc aggtcgaggc ctcgcgggct tccacgcgtg ccgttttccg
     5881 ccacttgccc tggccgagcc acgtcgcgaa cccgccaatg acgagcccaa gggcaaacgc
     5941 gccgagcagg aacaggaaca gaggggcctc gatgcccaag ccggcattgg cgtcaaacgg
     6001 attgaggccg agcatgaccg gcttgcggtt gacgacggcg agcgccacga gcagcacgcc
     6061 gatcggcaca agaatcaggg cggcgaccag tttacgcatc acggccgctc catcgggacg
     6121 aggccttaag cggccccatc ctcgcggttc aggcgctcgc gcagctcctt gccggtcttg
     6181 aagaagggca cgaacttgtc ctcgacaaag acggtgtcgc cggtgcgcgg attgcggccc
     6241 tgccgggcgg cacggtgctt caccgtgaag gcgccgaagc cgcgcagctc gacccggtct
     6301 ccgcgagcca gcgcgccagt tatttcatcg agcacgatgt tgatgatgcg ctcgacgtcg
     6361 cgctgaaaca ggtgcgggtt agccgcagcc acgcgctgaa tcaattccga cttgatcatg
     6421 attgccccac attgccggct tccccggcag cagcccccgc tagcttccag cagggtgcca
     6481 aagcgaaact agaccgtcaa gcctaagtcc ttccgaaact tgaccgaata gggacacgaa
     6541 gttgtcagcg gcaatgccga aagaagacgc gatcgagccg aacagccagc cccaaatgcc
     6601 gccgctttcg gacttcggct tccactcgac cacggaaagt cccggcgtga cgtcgcgctc
     6661 cttctgcagc cacctcttgg cagccctttc gtcgccgatc tcatcgacga gcttgagcgc
     6721 caaggcctgc cggccggaat agacgcggcc gtcggtcaag cccggcaccg ccgccggctc
     6781 gagattgcgg cgtttgccga caagatcgac gaaccagacc ttggcctcct gcaccatttc
     6841 cgcggcgagc gccctgccct tctcgtcggt cggctcgaag ggattgggaa ccgccttcaa
     6901 cggcccgctt ttcacctcct cgaccttgac cccgagcgtg cgcatgagct cggagacgtc
     6961 ggcccattgg aagatgacgc cgaccgagcc ggtgatggtg ttgccgtaga cgaagatgcg
     7021 gtcggtggcg agcgcgacga tataggcggc tgaggtggcg agcgtgccgc acaccgcgac
     7081 cactggcttc ttctcggcga ggcggcggat cgcgtcgtac atggcttctc cgccggtggt
     7141 ggtgccgccg gggctgttga tgtcgaggat cacggcccgc acctggctcg atttgccgac
     7201 cttgtcgagg agctcgagca tcttctgatc atcggtgatg aagccgctca ccgacacgcg
     7261 ggcgatatgc ggcaggaagc tgccggtgcc ggccaggcgc tggtcgccgg ccatgatcag
     7321 ggcgattacg aagacggcga gcacgaccgc ggcaagccgc caagcggtga gccgccgcct
     7381 gagacggcgg cgctcgatca cggtctcggc ttcgatcacg gtctcttctc tctagaagat
     7441 cgacagcctg gaaaggcgga ggtcagggca cggctcgcgc cgcagactat tcctcggcct
     7501 ccgccgaatc ctcggtcttg gcggactcgc tcttcgcctt gctcttgcgc ttcggcttct
     7561 cggcggcctc gtcgtcgtcc tcttccttcg ccttgctcag ggcggcaccg agaatgtcgc
     7621 cgagcgaggc gccggagtcg gtcgagccgt attgagcgac ggcctctttc tcctcggcga
     7681 tttcaagcgc cttgatggag acgccgacct tgcgcgcctt ggcgtcgacc tgggtcacca
     7741 cggcgtcgaa cttctcgccg acgccgaagc ggtcaggccg ctgctcggac cggtcgcgag
     7801 agaggtcgga gcggcggacg aagctcgtca ggtcgtggtc gacgagcttg acctcgagcc
     7861 cgctatcctt caccgcgacg acttcgcagg ttacgcgcat gcccttcttg agcttggcgg
     7921 acggcgcgcc ggaagcaaat ggatcggggg tgagctgctt gacgccgagg ctgatgcgct
     7981 ccttctcgga gtcggcttcg agcaccacgg ctttgacgtt gtcgcccttg ttgtagtcct
     8041 tgagggcgtc ctcccccgac ttggaccagt ccagatccga tagatgcacc atgccgtcga
     8101 tgccgccatc gagcccgatg aacagaccaa actcggtgat gttgcggatc gggccctcga
     8161 tattggtgcc gggcgggtga ttggcggcaa aggcgaccca gggattctcc tgggtctgct
     8221 tgagccccag cgaaatgcgg cgcttctcgg gatcgacctc gagcacctgc acctcgacct
     8281 cctggctggt cgagatgatc ttgccgggat ggatgttctt cttggtccag ctcatctccg
     8341 acacgtggat cagtccctca acgccgggct caagctcgac gaaggcgcca tagtcggtga
     8401 tgttggtgac ggtacctttg agccgcgagc cgaccgggta tttggcggca atgccctccc
     8461 aaggatcagc ttcgagctgc ttgatgccga ggctgatgcg ctgggtctcg ggattgatgc
     8521 ggatgatctg caccttgacg ctgtcgccga cattcacgac ctcgctcgga tgggcgacac
     8581 gcctccatgc catgtcggtg acgtgcaaca ggccgtcgat gccgccgaga tcgataaagg
     8641 cgccgtagtc ggtgatgttc ttgaccacgc cctcgatgac ctgaccctct tccagccgcg
     8701 ccacgatctc gctgcgcttc tcggcgcgcg tctcttcgag cacggagcgg cgcgacacga
     8761 cgatgttgcc gcggcggcga tccatcttca ggatctggaa gggctgcggc tggttcatca
     8821 gcggtccgac gtcgcggatc ggacggatgt cgacttggct gccaggcagg aaggccacgg
     8881 caccatcgag atcgacggtg aagccgcctt tgacgcggtt gaagatcacg ccttcgacgc
     8941 gctcgccggc tttgtatttt tcctcgagcc gcgtccagct ctcctcgcgt cgcgccttct
     9001 cgcgcgacag cacggcctcg cccatggcgt tctcgacgcg ctcaagatag acgtcgacct
     9061 cgtcgccgac ggagaggttg gcggcgcggc cggagatgcc aaattctttc aggggaatgc
     9121 ggccctcggt cttgaggccg acatcgatca ccgcaacgtc cttctcgatc ccgacgatgg
     9181 tgcccttgac gacgttgcct tcctgcagcg ccgtggtggt cagcgactct tcaagcaggg
     9241 ccgcgaactc gtcgtggctc ggatggctga tgctagctcg cggttcgttc ataaaaactc
     9301 tctagtccct tccaatcgga ttccccgtcc actgccggcg atggtgatcg cgagagcgcg
     9361 gggtcaagcg tgttcacggc tgccgtcacc gcatgaggtc taccgccaga atttcgcgga
     9421 aaacttctga aatggtgtgc gaaaggggtg ctgcctcaac cggcgtaccc tgcacgcgca
     9481 agcgcagcat cgatcaggtc gatggcagcc ctgaatgcgg cgtctatatc caaattcgta
     9541 gtatcgagca agtgcgcgtc aggggcccgg cgcaagggcg ccgcggcgcg gtgcttgtcc
     9601 cgttcgtcgc ggcggcggat atcgcactgc acttcggctt cacttacctc gattccggcc
     9661 tgtttcagct cgagatagcg ccgactggcg cgctcctcag gggtcgctgt cacaaatagt
     9721 ttcacgtcgg catctgggaa gatcaccgtg ccaatgtccc ttccgtcgag caccgctcct
     9781 gacttggcgc gggcgaagct ccgttgatag gcgagcagct cttcccgaac ctccggatgc
     9841 ttggccacga gcgaggccgc ttcggcgagg ccaggttttc ggaggcctgg gtcgccgagc
     9901 gtgaaagggt caagcgcctt ggcgcttttg agcgcctcga ttgcgtcggc cggatcttcg
     9961 ctgcgcgcca aggtgtcgcg cgccactgcg cggtagagcg tgccggtgtc gagataggca
    10021 aggccgtaat gcgcggcgac gcgcttggcg atcgtgccct tgccagaggc cgcaggccca
    10081 tcgatggcga cgatcatcgg aactgggctc cgagtttcgt catcagcggc acgaaggccg
    10141 ggaagctcgt ggcgatcatg ccgatatcgt cgacggtgac cggctgcccc gctccgaggc
    10201 cgagggtgag aaaggccatg gcgatgcggt ggtccatatg agtctccacc acgccgccgc
    10261 ccggcacact ccccttgccc atgacgatga gattgtcgcc ctcgatggcc gtctcgaccc
    10321 cgcaagcgac gagcccggcg gcggtggcgg caaggcggtc gctctctttc acgcgaagct
    10381 cggcaagacc acgcaagcga gtctcgccct cggcaaacgc ggcgagcacg gcgagcaccg
    10441 gatattcgtc gatcatgctc ggcgcgcgcc caggcggcac gtcaacaccg ctcagagcgc
    10501 cagcccgcac gcggagatcg gccgccggct cgccaccctc aaggcgaagg tcgagcaggt
    10561 caacgtctcc gcccatttct cgcaccgtct cgatgaagcc tgcgcgggtc gggttcatca
    10621 acacgccgtt gagcacaatg tcggagcctt cgcagatcag cgccgcggcg atggcgaagg
    10681 cggcagagct cgggtcacca ggaatggcga tggaggcgcc gctgagctcg gcatcgccgc
    10741 ggaccgtgac ggcgcggcgg ccgtcgtcct cgtccttcac cgagacctcg gcgccgaaat
    10801 aagtgagcat gcgctcggtg tggtcgcggg tgggaatagg ctcgatcacc gtggtgcggc
    10861 ccggcgcatg caggcctgcg agcagcaccg ccgacttcac ttgcgccgag gcgatcggag
    10921 attggtagac gatggggacg agatccggcg tgcccgtgat ggtgagcggg agcgtggttc
    10981 gcgtgtcgtc gccgacggca aggcccatgc gttgcagcgg cttgagcacg cgcgccatgg
    11041 gacggcggca aagcgaggca tcgccggtga agcgcgcctc catgtcgtgg ccggcgacca
    11101 cgccgagcat cagccgggag ccggtgccgg aattgccgaa gtcgagatcg tcgatcggcg
    11161 cctgcaggcc gccgacgcct tgaccggtca cctccaccga gccattgacg tggcggatcc
    11221 aggcgccgag cgccaccacc gcccttgccg tggccaagac atcctccgac tcgagcaggc
    11281 cggtgatgcg ggtgcgtcca gtcgccagcg cgcccagaat gagcgcgcga tgggagatcg
    11341 atttatcgcc gggaaccctg atcgtgccgg ctagggcccg cgtgggaacg gcggaaagtg
    11401 gttgcgacat ggaccttgga ttttcagtct ttgggcgctc tcgatagctt ttgacagcac
    11461 cagacaagcg tggcaatggg gggcgaagca catcaagcat cgaggacgtc aatgtcgaaa
    11521 cccgcgagag ggatcaagcg cgtttgccag agttgcggcg cgcggtttta cgacttgggc
    11581 cggacgccga tcgtctgccc ctcctgccag gccgtctatc aggtgacaca gccgacatcg
    11641 cggcgcggag agcgcgcgcc ggtgccggag gtacgggaaa aggtggagcc tgaggtcgag
    11701 gccgttgcgc tcgaaccgga gacgatcagc ctcgaagagg tcgaggagcc cggggaggaa
    11761 gctgcgatcg aagatgagga gatcgtcgat ctcggcgagg acgaggcgga aattcccgct
    11821 gctggcgaag acgacacctt cctggaggag ccagagggcg atgaggaggc cgatgtctcc
    11881 ggtattgtcg gcggccccga aaaggaggag ggctaggctg ggctattagc cgtaagccgg
    11941 aagccagaat ggcggttttc ggcttctggg tgcatatcgc atttctcttg aacgccgatt
    12001 gagaccgacc taattcaagc gccggagcca ggcacccgat ttctggcttc tgctttctga
    12061 tttttggttt ctgatcgggg ccatagctca gttgggagag cgcgtgaatg gcattcacga
    12121 ggtcggcggt tcgatcccgc ctggctccac caaattcccc aagcacttag ctggataccc
    12181 accttcctac aaaacccaat aggttgctta aaaggtagct gtgaggcaca cagcgcgtcc
    12241 atggctggct taagccgctt tggacgggcg cgacagaagg ccgctccatg ggctccccct
    12301 ggcccttgga tcaacagttc gttgctggcc gttttaagag gcatgtcacc caccaaggca
    12361 gaggggtatc gcggtcgcgc cgaggattgc tgtcgtaacg caatcatcgc cgaggacgag
    12421 cggcggcgct tacactggct agaagttgct tcgcgctgga ttaggctagc gcgtgaggag
    12481 ggcgtactgc ccgccgaata gagaggtacg agatgccgca agatcaatgc cagtgccaaa
    12541 gcgggaccca cggccatgag ccgggtgaat gcaaaaacgc catgactcac gaggacgatc
    12601 agatgtgtga ctactgccac gacaaggcag ccgatctcct cgcaatgctc ccacccggaa
    12661 aataaacttc tgctaagggc tacaaatcca cctaagcacg cgcgcctatc tacgggccgc
    12721 ttcccgcaag gcgtttatca gtggctcgcg atcaacgtct cccctccagc gagtcaactg
    12781 ttcgacgaat tcgagcattg attgaatctc agggtcggtc gcttcgcgct cgcgcatgtt
    12841 tgcgacctcc tttataaact cgacgaccaa ggactcggtg tttgcattcg tcatggaggc
    12901 aaccccctcg ttagtttccc cacaacgcat cgtctgtcta aaggtttcgc cgaccttgct
    12961 cgccggcagc ccggcggagc cccgtgctcc gtcgccatca tccaagccca cgaagcatcg
    13021 gtctggggcc ctcgcccctc ggaggcggaa cctaagtcgc
//
LOCUS       DATNAE010000209         4533 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_201987, whole genome shotgun sequence.
ACCESSION   DATNAE010000209 DATNAE010000000
VERSION     DATNAE010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4533
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201987"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1485
                     /gene="ligA"
                     /locus_tag="VN518_04825"
     CDS             <1..1485
                     /gene="ligA"
                     /locus_tag="VN518_04825"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HXE03930.1"
                     /translation="AEREKEGELVFANPRNAAAGSLRQLDSSITAKRPLAFFAYAWGE
                     QSEAFARSHADALKHFAAWGFTVNPLSKLCHGVDELLAFHRSIGEKRASLPYDIDGVV
                     YKVNDLDLEERLGFVSRAPRWAIAHKFPAQQAQTVLKKIGIQVGRRGTLTPVADLEPI
                     TVGGVVVSRATLHNEDEIKRKDIREGDTVIIQRAGDVIPQVVGVVEARRPRDSKPFKF
                     PHICPECGSKAVREEGEAAWRCSGGLICPAQAVERLKHFVSRNAFDIEGLGEKHIVDF
                     WNDDLIKGPADIFHLDAKKIAEREGWGEVSAKKLIAAIDARRDISLDRFVNALGIPQV
                     GEATAKLLARHYRSLSRWRKAMEEAAAPDSEAWHELNDIHGIGEDMAADIVGFFAEKH
                     NRTILDELAKELKVEDYAVAAPVGNSPFSGKTIVFTGTLTEMSRSEAKARAEALGAHV
                     SGSVSAKTDYLVVGADAGSKARKAAELGVKTLTEEEWLKLARQG"
     gene            1602..2858
                     /locus_tag="VN518_04830"
     CDS             1602..2858
                     /locus_tag="VN518_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXE03931.1"
                     /translation="MGKFEKSINRRTILKAGVAAGALQIAAPYIVKAQGEQPVKIGVD
                     NPLTGIYTALGKNENTGMALAAEQINAKGGILGRKVELVIEDSTSDKTDVAVQKARKL
                     IQSDKVDFLIGNVNSSQAIAISQVAAELKMPHIDPGGHADVITGSSCQWTTFRVCNTG
                     AMQGNAMAKPLVKIGKKIYIIAQDYAFGHSLADGLSKAAKTAGGTIVGTDFFPLGTTD
                     YSAILIKARAANPDVLVDLAAGDDGINSLKQAVQFGIDKQLHLAGMQQELEVLEGLPP
                     EARVGTWLFEWYWNQPSNVPGVADFVAGIRKSTGKVPTARTWFGFAAIHSFALAANKA
                     KTLDAAKVCHALEGLKLPPEVACQPGEPYYRAEDHQLVPAVFIGHAQAKGAKDPEDLF
                     VVDQVEQGPSFIPPATETGCKLHYPT"
     gene            2927..3796
                     /locus_tag="VN518_04835"
     CDS             2927..3796
                     /locus_tag="VN518_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXE03932.1"
                     /translation="MTQVILFNVLNGLIVGAFYALMALGLSLILNLSGVINFAHGGFL
                     ALGAYLAYSLAPYLGFWGGLVIAPFLTAALGIIVERVLIRRLYGRDPLYSLLLTFGLA
                     FIFQDGLRYTWGASSVPYQIPGWLGSPLSTSLFFLTGYRVFMVIIVALAVAALFLILG
                     YTRLGIRIRAGTLDLDTVSVLGVNVRVLRNLNFGLGIYLAGLAGVLAAGLLGLSPTIG
                     DQLIMPSFVAIIVGGLGSLPGTLAGGLLIGIAAGLTSVFFPTAQEAIIYVMMALVLMV
                     RPNGLFGDAGRVQ"
     gene            3793..>4533
                     /locus_tag="VN518_04840"
     CDS             3793..>4533
                     /locus_tag="VN518_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXE03933.1"
                     /translation="MKSLEGRTGPILLWLLLLTAPYWMPYVGGYTSLGTRVLVLALAA
                     MAVNFLLGFTGVLSFGHAAYFGLGAYGAGMAIKYIYPSSSLGMLIGVLAGTIGAMIIG
                     AMIVRLRGVYFAMVTIAFGQVFYFIAFRWVSVTGGDDGLTGWKRVPIDFGFTSFDILH
                     DDIAFYYLCLICLAISAAAMALLLRSPFGHSLLAIRENERRAWFLGIPVNRHIWMSFV
                     ISGFFASLAGVLYAYLDNFVDPRAFHWQQ"
BASE COUNT          808 a         1477 c         1374 g          874 t
ORIGIN      
        1 gccgagcgcg agaaagaagg cgagctggtc ttcgccaatc cgcgcaacgc cgcggccggc
       61 agcctgcgcc agctcgactc atcgatcacc gcgaagcgcc cgctcgcttt cttcgcctat
      121 gcctggggcg aacagagcga ggctttcgcc aggagccatg ccgatgcgct gaagcacttc
      181 gccgcctggg gtttcacggt caatccgctc tcgaagctct gccacggcgt cgacgagctc
      241 ttggcattcc atcgcagcat cggcgagaag cgggcctccc tgccctacga catcgacggc
      301 gtcgtctata aggtgaacga cctcgatctc gaagagcgcc tgggctttgt ctcgcgcgcc
      361 ccgcgctggg cgatcgcgca taaattcccg gcgcagcagg cgcagaccgt gctcaagaaa
      421 atcggcatcc aggtcgggcg gcgcggaacg ctgacgccgg tcgcagatct cgagccgatc
      481 accgtgggcg gcgtcgttgt cagccgcgcg accttgcaca atgaggatga gatcaaacgc
      541 aaggacatac gcgaaggcga cacggtcatc atccagcgcg ccggcgatgt catcccgcaa
      601 gtcgtcggcg tggtcgaggc gcggcggccg agggattcga aaccgttcaa attccctcac
      661 atctgtccgg aatgcggcag caaggcagtg cgcgaggaag gcgaagcggc ctggcgctgc
      721 agcggcggcc tcatctgccc ggcgcaagcg gtcgagcgcc tcaagcattt tgtttcgcgc
      781 aatgctttcg atatcgaagg cttgggagaa aaacacatcg tcgatttctg gaacgacgat
      841 ctgatcaaag gcccggctga tatcttccac cttgacgcaa agaagatcgc cgagcgcgaa
      901 ggctggggcg aggtcagcgc caaaaagctg atcgccgcca tcgacgcgcg ccgcgacatc
      961 tcgctcgacc gcttcgtcaa tgcgctcggc ataccgcaag tcggcgaagc aacggcaaag
     1021 ctcctcgcgc ggcactatcg cagcctctcg cgctggcgga aggcaatgga ggaagccgcc
     1081 gctcccgaca gcgaggcctg gcacgagctc aacgatatcc acggcatcgg cgaggatatg
     1141 gcggcggata tcgtcggctt cttcgccgag aaacataacc gcacgatcct cgacgagctg
     1201 gcgaaagagc taaaggtcga ggattacgcc gtcgccgccc cggtcggcaa ttcgcctttc
     1261 agcggcaaga ccatcgtctt caccggcacc ctgaccgaga tgtcgcgctc cgaagccaag
     1321 gcgcgggccg aggccctcgg cgcgcatgtc agcggctcgg tctcggcgaa gacggattac
     1381 ctggtcgtgg gcgccgatgc cggctccaaa gcgcgcaaag ccgccgagct gggggtcaaa
     1441 accctcaccg aagaagaatg gctgaagctt gcccgtcagg gataagacag cctacctgcc
     1501 cactttgggc cacaaagtcg cgggagcgtg aagcctcgtt gcttgcagcg ttttcgtttc
     1561 cacggtaatc aaaaggatac aaaaagctaa gggaggcctc cgtgggaaaa tttgaaaaga
     1621 gcatcaaccg ccgcacgatc ctcaaggcag gggtcgccgc gggcgcgctg cagatcgccg
     1681 cgccctatat cgtgaaggcg cagggcgagc agccggtgaa gatcggcgtc gataatccgc
     1741 tcaccggcat ctacaccgct cttggcaaga acgagaatac cggcatggcg ctcgccgccg
     1801 agcagatcaa cgcgaaaggc ggcattcttg gccgcaaagt cgagttggtg atcgaggatt
     1861 cgacctccga caagaccgac gtcgcggtgc agaaggcgcg taagctcatt cagagcgaca
     1921 aggtcgattt cctcatcggc aacgtgaact cctcgcaagc gatcgcgatc tcgcaggtcg
     1981 ccgcggagct caagatgccg catatcgatc ccggcggcca tgccgacgtg atcaccggat
     2041 cgagctgcca atggacgacg ttccgcgtct gcaataccgg cgcgatgcaa ggcaacgcca
     2101 tggcgaagcc gctcgttaag atcggcaaga aaatctacat catcgcccag gactacgctt
     2161 tcggtcattc gctcgccgac ggtctgagca aggccgccaa gacggcaggc ggtacgatcg
     2221 tcggtaccga tttcttcccg ctcggcacca ccgactactc ggcgatcctg atcaaggcgc
     2281 gcgccgccaa tcccgacgtg ctggtcgatc tcgctgccgg cgatgacggc atcaattcgc
     2341 tgaaacaggc ggtgcagttc ggcatcgaca agcagctcca cctcgccggc atgcagcaag
     2401 agctcgaagt gctcgagggt ctgccgcccg aagcccgcgt cggcacttgg ctcttcgaat
     2461 ggtactggaa ccagcccagc aacgtgccgg gtgtcgccga cttcgtggcc ggcatccgca
     2521 aatcgaccgg caaagtgccg acggcacgta cctggttcgg cttcgccgcc atccacagct
     2581 tcgcgctcgc cgccaataag gcgaagacgc tcgacgcggc gaaggtctgt cacgcgctgg
     2641 aaggcttgaa gctgccaccc gaagtcgcgt gccagccggg cgagccttac taccgtgccg
     2701 aagatcatca gctcgtgccg gccgtcttca tcggccatgc gcaagccaag ggcgcgaagg
     2761 accccgaaga ccttttcgtc gtcgaccagg tcgagcaggg cccgagcttc attccgccgg
     2821 ccaccgagac cggctgtaag ctccactatc cgacctgatc tctgtcgcag accgtgcgcc
     2881 gccgaataag ggacgcgcgg ttctccttta gggggtaggc ctatcgatga cccaagtgat
     2941 cctgttcaat gtcttgaacg ggctgatcgt cggcgcgttc tatgccctga tggcgctggg
     3001 gctgtcgctg atcctcaatc tcagcggcgt gatcaatttc gcgcatggcg gcttcctcgc
     3061 cctcggtgcc tatctcgcct acagcttggc gccctatctc ggcttttggg gcggcctcgt
     3121 catcgcgcca ttcctcactg cggcgttggg catcatcgtc gagcgcgtgt tgatccggcg
     3181 gctctatggc cgcgatccgc tctatagcct cctgctcact ttcggcctcg ctttcatctt
     3241 ccaggacggc ctgcgctaca cctggggcgc atcgagcgtg ccgtatcaaa ttccgggctg
     3301 gctcggctcg cccttgtcga cgagcctctt cttcctgacc ggctaccgcg tgttcatggt
     3361 catcatcgtc gccctcgccg tggctgcgct gttcctgatc ctcggttata cgcggcttgg
     3421 cattcgcatt cgtgccggca cgctcgatct cgacacggtc tcggtgctcg gcgtcaatgt
     3481 ccgcgttctg cgtaatctca atttcggcct cggcatctat ctcgccggtc tcgccggggt
     3541 gctcgccgcc gggctgctcg gtctctcgcc gacgatcggc gatcagctca tcatgccgag
     3601 cttcgttgcg atcatcgtcg gcggcctcgg tagcctgccc ggcacgctgg ccggcgggct
     3661 tctcatcggc atcgcggcgg gcctgacctc ggtgttcttc ccgaccgcgc aggaggccat
     3721 catctatgta atgatggcgt tggtgctgat ggtgcgtccg aacggcctct tcggcgatgc
     3781 ggggcgcgtg cagtgaaatc gctcgaagga cgtaccggcc cgatcctgct ctggctcttg
     3841 ctgctgaccg cgccttattg gatgccgtat gtcggcggtt acactagtct cggcacgcgg
     3901 gtactggtcc tcgccctcgc cgcgatggcg gtgaatttcc tcctcggctt caccggtgtt
     3961 ctgtccttcg ggcacgccgc ctatttcggc ctcggcgctt acggcgcggg catggcgatc
     4021 aaatacatct atccgagcag ctcgctcggc atgctgatcg gcgtgctcgc cggcacgatc
     4081 ggggcgatga tcatcggcgc gatgatcgtg cgattgcgcg gcgtctattt cgcgatggtg
     4141 accatcgcct tcggccaagt attctacttc atcgcgttcc gttgggtttc ggtgactggt
     4201 ggcgatgacg gcctcaccgg ctggaagcgc gtgccgatcg atttcggctt caccagcttc
     4261 gacatcctcc acgacgacat cgcgttctat tacctctgcc tgatttgcct ggcaatcagc
     4321 gcagcggcga tggcgctact gttgcgctcg cccttcggcc acagcctgct ggcgattcgt
     4381 gaaaatgagc ggcgcgcctg gttcctcggc attccggtca atcgccacat ctggatgtcg
     4441 ttcgtcatct ccggcttctt cgccagcctc gcaggcgtgc tctatgcgta tctcgacaat
     4501 ttcgtcgatc cgcgcgcctt ccactggcag caa
//
LOCUS       DATNAE010000210         2333 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_1107189, whole genome shotgun sequence.
ACCESSION   DATNAE010000210 DATNAE010000000
VERSION     DATNAE010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1107189"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..885)
                     /locus_tag="VN518_04845"
     CDS             complement(<1..885)
                     /locus_tag="VN518_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HXE03934.1"
                     /translation="MRSAPLPLPTAPRPQADIGKPFIIQSEFQPAGDQPQAIAALTQG
                     VVAGEREQVLLGVTGSGKTFTMAHVIARTQRPALILAPNKTLAAQLYGEFRSFFPENA
                     VEYFVSYYDYYQPEAYVPRTDTYIEKESSINEQIDRMRHAATRAIIERRDTIIVASVS
                     CIYGIGSVETYSEMTLLLEAGTRLNRTELLKRLVELQYRRNDQNFVRGAFRVRGDTIE
                     LFPAHYEDRAWRLSLFGDELEAIHEFDPLTGEKTTALEEVKIYPNSHYVTPKPTLYQA
                     IEQMKIDLKQRLAEFRAQG"
     gene            1032..1214
                     /locus_tag="VN518_04850"
     CDS             1032..1214
                     /locus_tag="VN518_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03935.1"
                     /translation="MDWEQVLGQCKGLGSRAKETWERLAREHWQERRMARDKARERAA
                     AAKEAQQHVKHFEDMH"
     gene            1369..>2333
                     /locus_tag="VN518_04855"
     CDS             1369..>2333
                     /locus_tag="VN518_04855"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HXE03936.1"
                     /translation="MPFLADRLQRIKPSPTLAMTKRSLEMKAAGRDVVGLAAGEPDFD
                     TPENIKQAAVAAMRRGETKYTAVDGTPELKKAIAGKFKRENDLDYAAGQITVGTGGKQ
                     VLFNALMATVNEGDEVILPAPYWVSYPDIVLLAEGTPIAVSCPQNNGFKLRPEDLEAA
                     ITAKTKWLILNSPSNPTGAAYSVAELRALADVLLRHPHVWVLTDDMYEHLVYDDFKFT
                     TIAQIEPKLYGRTLTLNGVSKAYCMTGWRIGYAGGPTELIKAIGTLQSQSTSNPSSIS
                     QAASVEALNGPQDFIPRNNAVFKERRDLVVEMLNRAPGIRCPRPEG"
BASE COUNT          411 a          747 c          787 g          388 t
ORIGIN      
        1 gccttgggcg cggaactcgg cgagccgctg cttcaggtcg atcttcatct gctcgatcgc
       61 ctggtagagc gtcggcttgg gcgtgacata gtggctgttg gggtagatct tcacctcttc
      121 gagcgccgtg gtcttctcgc ctgtgagcgg gtcgaattca tggatcgcct cgagctcgtc
      181 gccgaacagc gagaggcgcc aggcgcggtc ctcgtaatgc gccgggaaga gctcgatcgt
      241 gtcgccgcgc acgcggaagg cgccgcgcac gaaattctga tcgttgcgcc ggtattgcag
      301 ctcgaccagc cgcttcaaca gctcggtgcg gttgaggcgg gtgccggctt cgagcagcaa
      361 ggtcatctcg gaataggtct cgaccgagcc gatgccgtag atgcacgaga ccgaggcgac
      421 gatgatggtg tcgcgccgct cgatgatggc gcgcgtcgcg gcgtgccgca tccggtcgat
      481 ctgctcgttg atcgaggatt ccttctcgat ataggtgtcg gtgcgcggca cataggcttc
      541 gggctggtaa tagtcgtagt acgacacgaa atattcgacg gcgttctcgg gaaagaagct
      601 ccggaactcg ccatagagct gcgccgcgag cgtcttgttc ggcgcgagga tgagcgccgg
      661 gcgctgcgtc cgggcgatga catgcgccat ggtgaaggtc ttgcccgagc cggtgacgcc
      721 gagcagcacc tgctcgcgct cgcctgcgac gacgccttgc gtcagcgccg cgatcgcctg
      781 cggctgatcg ccggccggct ggaactcgga ttggatgatg aacggcttgc cgatatccgc
      841 ctgcggccgt ggcgccgtgg gcagcggcag aggagcggaa cgcatggtct ctaagatagg
      901 aaattcgccc cgaaggcgcc agggccgccc ccgctctgcc gggcccgacg ctgtcccatg
      961 acttggccct tgaccgccgt cgcgatcggg caaagctagg agatggcagc cttcggcgag
     1021 gggtagacgc catggactgg gaacaggtgc tgggccagtg caagggcctg ggcagccggg
     1081 ccaaggagac ctgggagcgg ctcgcccgcg agcattggca ggagcggcga atggcgcggg
     1141 acaaggcccg cgagcgtgcc gccgcggcca aagaggcgca gcagcatgtg aagcacttcg
     1201 aggatatgca ttagctccca tcccgtcggc ccggccatgg ggagcgcccg ccgaagggtt
     1261 gctgcaaaac cggtgcccgc aacgcaagct tcatgcgcgt atcgcagttg cgaagaccca
     1321 gcgccgggca tagcttgagg cctcgccttt ccaatacccg gtagcgccat gcccttcctt
     1381 gccgaccgcc tgcagcggat caagccgtcg ccgaccctcg ccatgaccaa gcggtccttg
     1441 gagatgaagg cggccggccg cgatgtcgtc gggctggccg cgggcgagcc cgatttcgac
     1501 acgcccgaga acatcaagca ggcggcggtc gcggcgatgc ggcgcggcga aacgaaatac
     1561 accgcggtcg atggcacgcc cgagctcaag aaggcgatcg caggcaagtt caagcgcgag
     1621 aacgatctcg actacgccgc cggccagatc accgttggca ccggcggcaa gcaggtgctg
     1681 ttcaacgcgc tgatggcgac ggtcaacgag ggcgacgagg tcatcctgcc ggcgccctat
     1741 tgggtctctt atcccgacat cgtgctgctc gccgagggga cgccgatcgc ggtctcctgt
     1801 ccgcagaaca acggcttcaa gctgcggccc gaggatctcg aggcggcgat caccgccaag
     1861 accaaatggc tcatcctgaa ctcgccctcg aacccgacgg gtgcggccta tagcgtcgcc
     1921 gagctgcgcg cgctcgccga tgtgctgctg cgccacccgc atgtctgggt gctcaccgac
     1981 gacatgtacg agcatctcgt ctatgacgat ttcaagttca cgacgatcgc ccagatcgag
     2041 cccaagcttt atggccgcac gctgacgctg aatggcgtct ccaaggccta ttgcatgacc
     2101 ggctggcgca tcggctatgc cggtggcccg accgagctca tcaaggccat cggtacgctg
     2161 caatcgcaat cgacctcgaa tccgagctcg atcagccagg ccgcctcggt cgaggcgctc
     2221 aacgggccgc aggatttcat cccgcgcaac aacgccgtct tcaaggagcg gcgcgacctc
     2281 gtggtcgaga tgctcaatcg cgcgccgggc atccgctgcc cgcggccgga ggg
//
LOCUS       DATNAE010000211         3144 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_506696, whole genome shotgun sequence.
ACCESSION   DATNAE010000211 DATNAE010000000
VERSION     DATNAE010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506696"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="VN518_04860"
     CDS             <1..350
                     /locus_tag="VN518_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HXE03937.1"
                     /translation="PVLPIVREAVERYLALCPIDLGSDDPLFVGVRGGPLSPRIIQLG
                     IARARGALGLPDTATPHALRHSFATHLLGAGADLRAIQELLGHASLSTTQGYTEVDRE
                     LLLKTYARAHPRA"
     gene            406..840
                     /locus_tag="VN518_04865"
     CDS             406..840
                     /locus_tag="VN518_04865"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HXE03938.1"
                     /translation="MTPGLGFAQSTSPSGVRTLNPPGAIKAEGTWSLGVRAGDFVFVA
                     GMQGVDPVTNSLVQDPQARIVQAFRNLKLIAESEGAGLKDCVRLTVYVSDLHRYAPMV
                     DKAQAELWGGPPYPPRTMVEVQRLFDDDIVEVDSMFYAPAKK"
     gene            1053..1727
                     /locus_tag="VN518_04870"
     CDS             1053..1727
                     /locus_tag="VN518_04870"
                     /inference="COORDINATES: protein motif:HMM:NF028169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXE03939.1"
                     /translation="MAETKSAERKQAKAKRRRDKKVRPQEIVAAAFEEFAEKGFAETR
                     LEDVAARAKVSKGLPYLYFKTKVELFKAVIRSVITSHFEVLRERMETTELSTEEFLKG
                     PFLSFIQELPGSRRAFIVRLLISEGYKHPELTQFYYENVISRGIETIRRLIDRGIERG
                     EFKPTPLRDYPQLLVAPVLMAILWRALFERHYHLDTNKLLATNIDLLVEAVKAPPRQS
                     AEGPGR"
     gene            1724..2716
                     /locus_tag="VN518_04875"
     CDS             1724..2716
                     /locus_tag="VN518_04875"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HXE03940.1"
                     /translation="MKRLIAFLVFVVIAGVVALLLTTFPANRETIFPGYMEADLVLVG
                     SEQGGRVETLSVEEGDSVEKDAPIFTLESTEQIAEVAAAKARVAEAEARLADARQQMQ
                     RPSEIDVLEASLAQAKAMLVQSNLNLDRTQTLFDKKWVSKAQLDDVIAQHDRNQAAVA
                     EAERRITAAKMPNRSDLIDAAAASVENARHSLEQAEKGLAKRKVFAPATGTVEEVYFR
                     PGEVVKEGQAVIALLPPGNLKVRFFVAEPVRASLHTGQTVKVSCDGCKGELTATISFI
                     AREAEFTPPVIFSQEQRQKLVFLVEARPSGEAAKLTAGQPVSVSLGPAAQLVQR"
     gene            2713..>3144
                     /locus_tag="VN518_04880"
     CDS             2713..>3144
                     /locus_tag="VN518_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013501326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXE03941.1"
                     /translation="MTDTKEIAIDAEGLTKSFNGRTVVNHLSLKVPKGEIYGFLGPNG
                     SGKTTTIRMLCGLLTPDSGHGTCLGYDILTQQGEIKREVGYMTQRFSLYEDLSIRENL
                     EFVARVYGVPNPRGAAKEAIHRLGLDGRAEQLAGQLSGGWKQ"
BASE COUNT          600 a         1042 c          982 g          520 t
ORIGIN      
        1 tcccagtgct tcccatcgtg cgcgaggcgg tcgagcgcta tctcgctctg tgccccatcg
       61 atctcggctc cgacgatccg ctgttcgtcg gggtgcgcgg aggtccgctc tctccccgca
      121 tcatccagct cggcatcgcg agggcgcggg gtgcgcttgg gctccctgac acggcgacgc
      181 cgcatgcgct ccgccactcc ttcgccacgc atctcctcgg cgccggcgcc gacttacgcg
      241 cgatccagga gctgctggga cacgcgagcc tctcgaccac gcaaggctat accgaggtcg
      301 accgcgagct cttgttgaag acctacgccc gcgcccaccc cagggcgtga taggatcacg
      361 aaaatgctga cgcgccttgc tcttctatgc ctagcgacgc tggctatgac gccgggactc
      421 ggctttgccc aatcgacatc gccaagcggt gtcaggactc tcaatcctcc cggcgccatc
      481 aaggccgagg ggacctggag cttgggcgtc agagccggag actttgtctt cgtcgccgga
      541 atgcaaggcg tcgatccggt gaccaacagt ttggtgcagg atccgcaagc gcgcatcgtt
      601 caagccttcc gcaatctcaa actgatcgcc gagtccgagg gcgcgggcct caaggattgc
      661 gtgcggctca ccgtctatgt aagcgacctg caccgctacg cgccgatggt cgacaaggcg
      721 caagccgagc tgtggggcgg cccgccctat cctccccgga ccatggtcga ggtccaacgg
      781 ctgttcgacg acgatatcgt tgaggtcgac agcatgtttt acgccccggc gaagaagtag
      841 ccggacccgg ccccgctctc ccgaaagggc cgtctcccgc tcgccggctc gatgccggtc
      901 aaggttgcgc cgcctcaacc aatacgaaaa taccaggact ttccccgctt cgcccattgc
      961 ggtactgacc ggtcagtcat tatattgacc gagatcaagg ctgaaaggtg gccaaggccg
     1021 ccaagatcgg ccgcaaccca agggcgggag ccatggcaga gaccaaatca gcggagcgga
     1081 agcaggccaa ggcgaaacgg cggcgcgaca aaaaggttcg cccgcaagag atcgttgccg
     1141 cggccttcga ggaattcgcc gagaagggtt ttgccgagac aaggctcgag gatgtcgccg
     1201 cccgcgccaa ggtgagcaag ggccttccct atctctactt caagaccaag gttgagctgt
     1261 tcaaggcggt gatccgcagc gtcatcacct cgcatttcga ggtactgcgc gagcgcatgg
     1321 agacgaccga gctctccacc gaggaatttc tcaaaggccc cttcctctcc ttcatccagg
     1381 agcttcccgg ctcgcgccgc gccttcatcg tgcggctact gatctcggag ggctacaagc
     1441 atcccgagct cacgcaattc tattacgaga acgtcatctc gcgcgggatc gagacgattc
     1501 ggcgcctcat cgatcgcggc atcgagcgcg gcgagttcaa gccgacaccc ttgcgcgact
     1561 atccgcagct cctcgtggcg ccggtgctga tggcgatcct gtggcgggcg ctgttcgagc
     1621 gccactacca cctcgacacc aacaagctgc tcgccaccaa tatcgatctg ctggtcgagg
     1681 ccgtcaaagc gcctccccgc cagagtgccg aaggacccgg gcgatgaagc gcctcatcgc
     1741 cttcctggtc tttgtcgtga tcgccggcgt cgtcgccttg ctgctgacga ccttcccggc
     1801 caaccgcgag acgatctttc cgggctacat ggaagccgac ttggttctgg tcggctccga
     1861 gcaaggcgga cgcgtcgaga cactgtcggt ggaggagggc gatagcgtcg agaaggacgc
     1921 cccgatcttc acgctggaaa gcaccgagca gatcgccgag gtcgccgcag ccaaggcgcg
     1981 ggtcgccgag gccgaggctc ggctcgccga cgcgagacag cagatgcagc ggccgagcga
     2041 gatcgatgtg ctcgaagctt cgctcgcgca agccaaagcc atgctggtgc aatcgaatct
     2101 caacctcgat cgcacgcaga cgctgttcga caagaagtgg gtgtcgaagg ctcagctcga
     2161 cgatgtgatc gcccagcatg accgtaacca ggccgcggtc gccgaagcgg agcgtcgcat
     2221 caccgcagcc aagatgccga accgctccga cctcatcgat gcggcggcag cttcggtcga
     2281 gaacgcgcgg cattcgctgg agcaagccga gaaaggcctc gccaagcgga aggtgttcgc
     2341 gccagcgacc gggacggtcg aagaggttta tttcaggccc ggcgaggtgg tgaaagaggg
     2401 ccaggcggtg atcgcgcttc tgccgccggg caacctcaag gtgcgattct tcgtggctga
     2461 gccggtgcgg gcgagcctgc acaccggcca gaccgtcaag gtcagttgcg acggctgcaa
     2521 gggcgagctc accgccacga tcagcttcat cgcgcgcgag gcagagttca ccccgcctgt
     2581 gatcttctcg caggagcagc ggcagaagct cgtattcctg gtcgaggcga ggccctcagg
     2641 cgaagcggcg aagctcaccg ccggccagcc ggtgagcgtg agccttgggc cggctgcgca
     2701 actggtgcag cgatgaccga caccaaggag atcgccatcg acgccgaggg gctgaccaag
     2761 tccttcaacg gccgcaccgt ggtcaaccat ctctccctca aggtgccgaa gggcgagatc
     2821 tacggcttcc tcggcccgaa cggcagcggg aagaccacca ccatccgcat gctgtgcggg
     2881 ctgctgacgc cggactcagg acacggcacc tgcctcggct acgacatcct cactcagcag
     2941 ggcgagatca agcgcgaggt cggctacatg acccagcgct tcagcctcta tgaggacctc
     3001 tccatccgcg agaacctcga attcgtggcg cgcgtctatg gcgtgccaaa cccaagaggt
     3061 gctgccaagg aggccatcca ccggctcggg ctcgacggcc gcgccgagca gcttgccgga
     3121 caattgtctg gaggctggaa gcaa
//
LOCUS       DATNAE010000212         2069 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2924114, whole genome shotgun sequence.
ACCESSION   DATNAE010000212 DATNAE010000000
VERSION     DATNAE010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2924114"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VN518_04885"
     CDS             complement(<1..334)
                     /locus_tag="VN518_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03942.1"
                     /translation="MSPYDLIGAPQLAALLVLGQRGLEELYSARNTRRLMASGATEMG
                     GTYYPVVAATHLAWIAGLFFLIPPAAPIVWPLIAYYAALQVVRYWVIASLGPFWTHRI
                     ITLPGAPIS"
     gene            complement(405..>2069)
                     /locus_tag="VN518_04890"
     CDS             complement(405..>2069)
                     /locus_tag="VN518_04890"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXE03943.1"
                     /translation="AVLAPDQTPLAVGGKLTPAALAALLGQADNKAALAQSVGDAAVV
                     AAALPGDNHLRLAFVPAMDNGKVARVYAFVVDQTAAAGLTNLALTVVTLTTSLLIVMG
                     FSVPAAIASRRIRERWLAEDQIRYLAMHDSLTGLPNRLQLRQHLDRAVARSKRHGHLM
                     AVFCLDLDRFKDVNDTLGHPTGDALLAEVSARLRESVREVDLVGRLGGDEFAIVAEDL
                     EAPEDAMRLARRVCTALGETYHVNGHEVTSSASIGIAIGPLDNQAPETLLKNADLALY
                     RAKEDGRNTFRFFEPAMDAALQKRRRLENDLRNALRKNQLYLDYQPQFDLVSGRLTGY
                     EALVRWWHPSEGEVPPTTFIPIAEETGLIVPLGEWILKTACAYATTWPDDTKLAVNLS
                     PAQFKTQDIYGLVRRVLSETGLPPQRLELEITEGIILQNTEAVLDTLTRLDQLGVSIA
                     MDDFGTGYSSLSYLTRFPVKKIKIDRSFIDTLGTSPQTSAIVSSIVGLGQSLNVTITA
                     EGVETEGQAAMLRKWGCDQVQGFYYGRPDADVPESEEQVAKQPQVA"
BASE COUNT          355 a          627 c          747 g          340 t
ORIGIN      
        1 gggagatcgg cgctccggga agcgtgatga tgcggtgggt ccagaacgga ccgaggctgg
       61 cgatcaccca atagcgcacg acctggagcg ccgcatagta ggcgatcagc ggccagacga
      121 tcggcgccgc gggcgggatc agaaagaaca atccggcgat ccaagcgaga tgcgtggcgg
      181 ccacgacggg atagtaggtg ccgcccatct cggttgcccc cgacgccata aggcgccgcg
      241 tgttgcgcgc cgagtagagc tcctcgaggc ctcgctggcc gagcacgagc agggcggcaa
      301 gctgcggggc gccgataagg tcgtaaggac tcatgggccg cggacactca acccaaacta
      361 aggcgggggt tggtccgcgt tagccgcaag cggggggact tgcgctaggc gacttgcggc
      421 tgctttgcga cctgctcttc ggactccggt acatccgcgt cgggcctgcc gtaatagaag
      481 ccctgcactt gatcgcagcc ccacttcctc agcatggcgg cttggccttc cgtctccacc
      541 ccctcggcgg tgatcgtcac gttgagcgac tggccgaggc cgacgatgct cgagacgatg
      601 gccgaggtct gcggactggt gcccagcgta tcgatgaagg agcgatcgat cttgatcttc
      661 ttgaccggga agcgggtcag gtaggaaagg cttgaatagc cggtgccgaa atcgtccatg
      721 gcgatcgata cgccgagctg gtcgagccgg gtgagcgtat cgagcaccgc ctcggtgttc
      781 tggaggatga tgccttcggt gatttcgagc tcgagtcgct gcggtggaag cccggtctcg
      841 ctcagcaccc ggcgcacgag cccgtatatg tcctgggtct tgaactgcgc cggcgagaga
      901 ttgacggcga gcttggtgtc atccggccag gtggtggcgt aggcgcaagc cgttttcaga
      961 atccactcgc ccagcggcac gatgagaccg gtttcctcgg cgatggggat aaacgtggtg
     1021 ggcgggacct cgccctcgct cggatgccac cagcgcacca gcgcctcgta gccggtgagc
     1081 ctgccgctca ccaagtcgaa ctggggctga tagtcgagat aaagctggtt cttgcgcagc
     1141 gcattgcgca gatcgttctc cagccggcgc cgtttctgca gcgccgcgtc catggccggc
     1201 tcgaagaagc ggaaggtgtt gcggccatcc tccttggccc ggtagagcgc caggtcggcg
     1261 ttcttgagca gcgtctccgg cgcctggttg tcgagcgggc cgatggcgat gccgatcgac
     1321 gcggaactcg tcacctcgtg gccgttgaca tgataggtct cgccgagcgc ggtgcagacg
     1381 cggcgggcga gccgcatggc gtcttccggc gcttcgagat cctcggcaac gatggcgaac
     1441 tcatcgccgc ccaagcggcc gacaagatcg acctcgcgca cgctctccct gaggcgggcc
     1501 gagacttcgg cgagcaaggc atcgccagtc gggtggccaa gcgtatcgtt gacgtccttg
     1561 aagcggtcga ggtcgaggca gaacaccgcc atcaggtggc catgccgctt ggagcgagcg
     1621 acggcgcgat cgagatgctg gcgcagctga agccgattgg gcaggccggt gagcgaatcg
     1681 tgcatggcga ggtagcggat ctggtcctcg gcgagccagc gctcgcggat gcgccgcgag
     1741 gcgatggcgg cgggcacgga gaagcccatg acgatcaaca ggctggtcgt cagcgtgacg
     1801 acggtcagcg cgagattggt aagtccggca gccgcggtct gatccaccac gaaggcgtag
     1861 acgcgggcga ccttgccgtt atccatcgcc gggacgaagg cgaggcgcag atggttgtcg
     1921 ccgggcaggg ctgccgccac cacggcggcg tcgcccacgg actgcgccag cgcggccttg
     1981 ttgtcggctt ggccgagcag cgcggcaagg gcggcaggcg tgagcttgcc gccgacggcg
     2041 agcggggtct gatcgggagc gagtacggc
//
LOCUS       DATNAE010000213         2287 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3217092, whole genome shotgun sequence.
ACCESSION   DATNAE010000213 DATNAE010000000
VERSION     DATNAE010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3217092"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2170
                     /gene="clpB"
                     /locus_tag="VN518_04895"
     CDS             <1..2170
                     /gene="clpB"
                     /locus_tag="VN518_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014282990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HXE03944.1"
                     /translation="LRKGRTADSAGAEQSYDALKKYTRDFTEAAAKGKLDPVIGRDEE
                     IRRTIQVLSRRTKNNPVLIGEPGVGKTAIVEGLAHRIVAGDVPESLKDKKVLALDMGS
                     LIAGAKYRGEFEERLKAVLSEIEASHGQIILFVDEMHTLVGAGKAEGATDASNLLKPA
                     LARGELHCVGATTLDEYRKHVEKDAALARRFQPVYVSEPTVEDTISILRGLKEKYELH
                     HGVRISDAALVAAAMLSNRYISDRFLPDKAIDLVDEAAARLRMQVDSKPEELDELDRR
                     IMQLKIEREALKKEKDAASTDRLAKLEKELVNLEEKANALAQRWQEEKSKLASAQKIK
                     EQLDQARIELEQAQRRGDLAKAGELAYGVIPDLEAKLKKIEKEESKSGVMAQEVVTPD
                     LIAQVVSRWTGIRVDKMLEGEREKLLRMETQIAKRVVGQEEAVEAVAKAVRRSRAGLQ
                     DPNRPIGSFMFLGPTGVGKTELSRALAEFLFDDEHAMIRIDMSEYMEKHSVARLIGAP
                     PGYVGYEEGGALTEAVRRRPYQVILFDEIEKAHPDVFNVLLQVLDDGRLTDGQGRTVD
                     FRNTLIIMTSNLGSEYLVNQPEGEDSDAVREQVMNVVRAHFRPEFLNRVDEIILFHRL
                     KREHMGKIVDIQMQRLAKLLADRKITLVLDDAAKEWLGKKGYDPAYGARPLKRVIQKY
                     VQDPLAEMVLAGKIHDGETVKVSAGKDGLMFNRTAAKKAA"
BASE COUNT          459 a          732 c          762 g          334 t
ORIGIN      
        1 tcttcgcaag ggccgcaccg ccgactcggc cggcgccgag cagtcctacg atgcgctgaa
       61 gaaatacacg cgcgacttca ccgaggccgc ggccaagggc aagctcgacc ccgtcatcgg
      121 gcgcgacgag gaaatcaggc gcaccatcca ggtgctgtcg cgccgcacca agaacaaccc
      181 cgtgctgatc ggcgagccgg gcgtcggcaa gacggccatc gtcgagggcc tggcgcaccg
      241 catcgttgcg ggcgacgtgc cggagagtct gaaagacaag aaggtgctcg ccctcgacat
      301 gggctcgctc atcgccggcg ccaaatatcg cggcgagttc gaggagcggc tgaaagccgt
      361 gctgtcggag atcgaggcca gccacggcca gatcatcctg ttcgtcgacg agatgcacac
      421 cctggtcggc gcgggcaagg ccgagggcgc cacggacgcg tccaacctgc tcaagcctgc
      481 gctcgcccgc ggcgagctcc actgcgtcgg cgccaccacg ctcgatgaat atcgcaagca
      541 tgtggagaag gacgcggcgc tcgcgcggcg tttccagccg gtctatgtgt cggagcccac
      601 ggtcgaggac accatctcga tcctgcgcgg cttgaaggag aaatacgagc tgcatcacgg
      661 cgtgcgcatc tccgacgccg cgctcgtcgc cgccgccatg ctctccaacc gctacatcag
      721 cgatcgcttc ctgcccgaca aggcgatcga cctcgtcgac gaggcggcgg cgcggctccg
      781 catgcaggtc gattccaagc ccgaggagct cgacgagctc gaccggcgca tcatgcagct
      841 caagatcgag cgcgaggcgc tgaagaagga aaaggatgcc gcatcgacgg accggctcgc
      901 caagctcgag aaggagctgg tcaatctcga ggagaaggcg aacgcgctcg cccagcgttg
      961 gcaagaggag aagtcgaagc tcgcctcggc ccagaagatc aaggagcagc tggaccaggc
     1021 ccgcatcgag ctcgagcagg cgcagcggcg cggcgatctc gccaaagccg gcgagctcgc
     1081 ctatggcgtc atccccgacc tcgaagccaa gctgaaaaag atcgagaagg aggagtcgaa
     1141 gagcggcgtg atggcgcagg aggtggtgac gccggacctc atcgcccagg tggtgtcgcg
     1201 ctggaccggc atccgcgtcg acaagatgct cgaaggcgag cgcgagaagc tgctgcgcat
     1261 ggaaacgcag atcgctaaac gcgtggtcgg ccaggaggag gcggtcgagg cggtggccaa
     1321 ggccgtgcgc cgctcgagag ccggcctgca agaccccaac cgccccatcg gctccttcat
     1381 gttcctaggg ccgaccggcg tgggcaagac cgagctctcc cgcgcgctcg ccgagttcct
     1441 gttcgacgac gagcacgcca tgatccgcat cgacatgagc gaatatatgg agaagcactc
     1501 cgtggcgcgg ctgatcggcg ccccgcccgg ctatgtcggc tacgaagagg gcggtgcgct
     1561 caccgaggcg gtgcgccggc gcccctatca ggtcatcctg ttcgacgaga tcgagaaggc
     1621 gcatccggac gtgttcaacg tgctgctcca ggtgctcgac gacggcaggc tcaccgacgg
     1681 ccagggccgc accgtcgatt tccgcaacac gctcatcatc atgacctcca atctcggctc
     1741 ggaatatctc gtcaaccagc cggagggcga ggactctgac gccgtgcgcg agcaggtgat
     1801 gaatgtggtg cgcgcccatt tccgccccga gttcctgaac cgcgtcgacg agataatcct
     1861 gttccaccgg ctgaagcgcg agcatatggg gaagattgtc gacatccaga tgcagcggct
     1921 tgcgaagctg ctcgccgacc gcaagatcac gctcgttctc gacgatgcgg ccaaggagtg
     1981 gctcggcaag aagggctatg acccggctta cggcgcgcgg ccgctcaagc gcgtgatcca
     2041 gaaatacgtg caggacccgc tcgccgagat ggtgcttgcc ggcaagatcc atgacgggga
     2101 gacggtgaag gtgagcgccg gcaaagacgg gctgatgttc aatcgcaccg ccgcgaaaaa
     2161 agcggcttag gtcatccttc ctcgatgggg taagcacata gtaagcgccg gcgattctgt
     2221 tggcgccgag tcggcattgc actgggtgga cgaaggctta cgccgctgca catcgccttt
     2281 ggggcgg
//
LOCUS       DATNAE010000214         2307 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2616333, whole genome shotgun sequence.
ACCESSION   DATNAE010000214 DATNAE010000000
VERSION     DATNAE010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2616333"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..965
                     /locus_tag="VN518_04900"
     CDS             <1..965
                     /locus_tag="VN518_04900"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HXE03945.1"
                     /translation="QIAAASRLFRRRIIVKAADGLERIAEADTVVFDKTGTLTLGRPQ
                     LIEDERISDDALAAAAGLACASKHPYARAIVAAAEQRLGRAVPASGAEEVPGCGLKRS
                     LKQGEERIGSAEWCGVESGGEESEVWYRRDSQAPVRFRFDDRVRSDAGDVIAALRDRG
                     YRLALLSGDRIAVVERIAREVGIETWRAELKPSDKIAWLDQRAAEGCKVLMIGDGLND
                     APALAAAHASMSPASAADISQRAADFIFQGEKLAPILDAISTAKRARSMALQNFAVAL
                     VYNVVCVPLAMAGMVTPLIAAIAMSTSSILVTANAARLAGRGSP"
     gene            962..1129
                     /gene="ccoS"
                     /locus_tag="VN518_04905"
     CDS             962..1129
                     /gene="ccoS"
                     /locus_tag="VN518_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HXE03946.1"
                     /translation="MTALAWLVPAALLLGAFGLAAFMWALRTGQFEDLEGAAYRALED
                     DPLEDDDIDKK"
     gene            1126..2022
                     /locus_tag="VN518_04910"
     CDS             1126..2022
                     /locus_tag="VN518_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03947.1"
                     /translation="MRGLKDIDYVLAKLAFMRAERIWPNGLRYLWTDAFGLVLLVSLY
                     DELKEQRFLDEALQLVAEVERVLGRKRGIRIGEEPDRDGQYFHYLAMWLYALAVLGRY
                     APSYREKGIALARDIHGPFVVPGRGVMWKMKEDLSGPYPGYGFGALDAFDGYVSYRLL
                     DEQRLAQQIGEMRTIIDRTAGDLVITQDLGLGMMLWMTQFFPEEWWAGLQRQRSLAVL
                     DRMWMEEGYFAREPYQPEMRFAFTNYGVSVGLQAAGVMPDRVQRLNAYFETYRAGDDY
                     DRAAITHVMACNSHFPGLLLAP"
     gene            complement(2073..>2307)
                     /locus_tag="VN518_04915"
     CDS             complement(2073..>2307)
                     /locus_tag="VN518_04915"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HXE03948.1"
                     /translation="LPIGGVKNKVLAAVRAGITTVMLPERNKKDFEDVPEAARNAVRF
                     VWLANVDDAIEAALEPLAVQQAGRPSPPLAASA"
BASE COUNT          372 a          731 c          778 g          426 t
ORIGIN      
        1 tgcagatcgc cgccgcgagc cggctctttc gtcgccggat catcgtcaag gccgccgacg
       61 ggctcgagcg catcgccgag gcggacacgg tcgtgttcga caagaccggc acgctgaccc
      121 tcgggcggcc gcaacttatt gaggacgaac gcatttccga cgatgccttg gcggctgccg
      181 ccgggctcgc ttgcgcgagc aagcatcctt acgcgcgtgc gatcgtcgcc gccgcggaac
      241 agcgcttggg gcgggcggtg cccgcatcgg gggccgagga agttcccggc tgcggtctga
      301 agcgatcgct aaagcaaggc gaagagcgga tcggctccgc cgagtggtgc ggcgtcgaaa
      361 gcggcgggga ggaaagcgag gtctggtaca ggcgcgactc gcaggcgccg gttcgcttcc
      421 gcttcgatga ccgcgtgcgt agcgatgccg gcgacgtgat cgcggcgttg agggaccgcg
      481 gctatcggct tgccctcctc tcgggggatc gaattgccgt ggttgaacgg atcgcgcgcg
      541 aggtcggcat cgagacctgg cgcgccgagt tgaagcctag cgacaagatt gcctggctcg
      601 atcagcgcgc ggcggaagga tgcaaggtgc tgatgatcgg cgacgggctg aacgatgcac
      661 cggcgctcgc cgcggcccat gcctcgatgt cgcctgcgag cgccgccgat atcagccagc
      721 gtgcggccga tttcatcttt cagggggaga agctcgcgcc gatcctcgac gccatctcca
      781 cggcgaaacg ggcgcggtcg atggccttgc aaaacttcgc ggttgccctg gtctataatg
      841 tcgtttgcgt gccgctcgcc atggccggca tggtcacgcc gctgatcgcc gccatcgcca
      901 tgtcaacgtc atcgatcctg gtcacggcca acgccgcgcg cctggcgggg aggggctctc
      961 catgacggcg ctcgcctggc tcgtgcctgc ggcactgctg ctcggcgctt tcggtctcgc
     1021 tgcattcatg tgggcgctcc gcacgggtca attcgaggat ctcgaaggcg ccgcctatcg
     1081 agcgctggaa gacgatcccc tcgaagatga cgacatcgac aaaaaatgag aggtctcaag
     1141 gacatagact atgtcctcgc caagcttgcc ttcatgcgtg cggagcgcat ctggccgaac
     1201 ggactgcgct atctctggac cgacgccttc ggtctcgtcc tcctcgtctc tctctatgat
     1261 gagctgaagg agcagcgctt cctcgacgaa gccctgcagc tcgtggccga ggtggagcgc
     1321 gtgctcgggc ggaagcgcgg catccgcatc ggtgaagagc ccgatcgcga cggccagtat
     1381 ttccattacc tcgccatgtg gctctatgcc ttggcggtgc ttgggcggta cgcgccgtca
     1441 taccgggaga agggcatcgc cttggcgcgc gacattcacg gtcccttcgt ggtgcctggc
     1501 cgcggcgtga tgtggaagat gaaggaggat ctgagcgggc cataccccgg ctacggcttc
     1561 ggcgcgctcg atgccttcga cggctatgtc tcctaccggc tgctcgacga gcagcgtctg
     1621 gcgcaacaga tcggcgaaat gcggacgatc atcgatcgga cggcgggcga tctcgtcatc
     1681 acccaggatc tcggactggg catgatgctt tggatgacgc agttcttccc cgaggagtgg
     1741 tgggccgggc tgcaaagaca gcggtcgctc gccgttctcg accgcatgtg gatggaggag
     1801 gggtatttcg cgcgcgagcc ttatcagccc gagatgcgct ttgccttcac caattacggt
     1861 gtctcggtgg gattgcaggc ggccggcgtc atgccggatc gcgtgcagcg actcaacgcc
     1921 tatttcgaga cctatcgcgc gggtgacgac tatgaccgcg ccgcgatcac ccatgtgatg
     1981 gcctgcaact cgcattttcc gggattgctg ctcgccccct gattttcgtc cttcggtgga
     2041 cgagggcgaa gacaagcttc aggcgccgaa atctacgcgc tggcggcaag cggcggcgag
     2101 gggcgcccgg cctgttgtac agccagaggc tcgagcgccg cctcgatcgc atcgtcgaca
     2161 ttggcgagcc agacgaagcg cacggcattg cgcgccgcct ccggcacgtc ctcgaagtcc
     2221 ttcttgttgc gctcgggcag catcaccgtg gtgatgcccg cgcgcaccgc cgccagcacc
     2281 ttgttcttga cgccgccgat cggcagc
//
LOCUS       DATNAE010000215         3532 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_1720610, whole genome shotgun sequence.
ACCESSION   DATNAE010000215 DATNAE010000000
VERSION     DATNAE010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1720610"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="VN518_04920"
     CDS             complement(<1..765)
                     /locus_tag="VN518_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXE03949.1"
                     /translation="MAATDTHRAIDAVWRIEQAKLIAGLTRIVRDIGLAEELAQDALV
                     AALEQWPKSGIPDNPGAWLMAAAKHRAIDVFRRNSLRERKHEQLGYELEVEQETGQPD
                     LDAALDDQVGDDLLRLVFIACHPILSPEARAALTLRLLGGLSTDEIARAFLISEPTVQ
                     QRIVRAKRTLSESRVPFEVPRGDVLEARLSSVLSVIYLIFNEGYSATAGEDWMRPQLC
                     QEALRLGRILAELAPKQAEVHGLVALMEIQASRFGAR"
     gene            complement(808..1215)
                     /locus_tag="VN518_04925"
     CDS             complement(808..1215)
                     /locus_tag="VN518_04925"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HXE03950.1"
                     /translation="MRFLSMVKMNEAKMTPTPKAMMDAINAMVEEAAKDGCVMVQGVG
                     LLPTSSGMRARLAGGKLTLTDGPFTEAKEVVGGYAIFECASKAQMRKWTERFMELHKK
                     HMPGWEGECEVRQIPDPGQEVCGQGAEQRAAAE"
     gene            complement(1248..1658)
                     /locus_tag="VN518_04930"
     CDS             complement(1248..1658)
                     /locus_tag="VN518_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018427766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HXE03951.1"
                     /translation="MRFMIVVKATKESEAGVKPPEELFAAMADYHEKLAKAGVLLDAS
                     GLQPTSKGWRVKYSGGKRTIVDGPFTEAKELIAGYTMIQVKSREEAIEWSRRFPNPTL
                     DGRDCEIEVRQLFELEDFGESEALDRFRELALQK"
     gene            complement(1793..2062)
                     /locus_tag="VN518_04935"
     CDS             complement(1793..2062)
                     /locus_tag="VN518_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HXE03952.1"
                     /translation="MTQTTGRFFDELGKLITGAAGAADGVRKEIETVVRAQAERVLRD
                     LDVVQREDFEAVKAMAQKAREENELLKQRIAALEAAIVKGLADKA"
     gene            2192..3019
                     /gene="lgt"
                     /locus_tag="VN518_04940"
     CDS             2192..3019
                     /gene="lgt"
                     /locus_tag="VN518_04940"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HXE03953.1"
                     /translation="MELLVIPYPVIDPIAIEFGPVAVRWYGLAYMAGILLGWLYGRRL
                     VSRPDLWGGKPPLTVTQADDFLLWITLGIVVGGRLGFVLFYEPSYFWQNPVEIPAVWH
                     GGMSFHGGLLGVVLAVYLYSRVQGINPLSLGDIASAATPFGLFFGRIANFINAEVVGR
                     VSEVPWAMVFPGAGDQPRHPSQLYEAFLEGIVLFIILRIATHKFHALTRPGTVFGLFL
                     VFYGIFRSAVEFVREPHAGHPMDVGIFTPGIVYSIPMILIGAWLIWRARRKPALASS"
     gene            2971..>3532
                     /locus_tag="VN518_04945"
     CDS             2971..>3532
                     /locus_tag="VN518_04945"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HXE03954.1"
                     /translation="MADLAGETEACPGVVVMGAQRKPHRAEERPLAARLQQLIARNGP
                     ISVHDYMQACLADARAGYYPTRQPIGKDGDFVTAPEISQMFGELLGVWAVAVWQAMGE
                     PAVVIAELGPGRGTLIADALRVFGRVPGLRDRVTIALVETSPSLRETQRATLRSSPAP
                     LLWCEDIGEVPQGPLIVIANEFVDALP"
BASE COUNT          529 a         1185 c         1141 g          677 t
ORIGIN      
        1 gcgcgctccg aagcgcgatg cctggatctc catcagcgcg acgaggccgt gcacctccgc
       61 ctgcttcggc gccagctcgg ccaggatgcg gccgagacgc agcgcctctt ggcagagctg
      121 aggccgcatc cagtcctcgc cggccgtggc cgagtagcct tcgttgaaga tgagatagat
      181 gacggacagc accgaggaca gacgcgcctc gagcacatcc ccgcgcggca cttcaaaggg
      241 cacgcggctc tcgctcagcg tgcgcttggc gcggacgatg cgctgttgga cggtcggctc
      301 ggagatgagg aacgcgcgcg cgatctcgtc cgtgctcaag ccgccgagca ggcgcaacgt
      361 cagcgccgct cgtgcttcag gcgaaaggat cgggtggcag gcgatgaaca cgaggcggag
      421 cagatcgtcg cccacctgat cgtccaatgc cgcgtcgaga tccggttgcc ccgtctcctg
      481 ctcgacttcg agctcgtagc cgagttgctc atgcttgcgt tcgcgcaagc tgttgcggcg
      541 gaagacgtcg atcgcccggt gcttcgcggc ggccatgagc catgcgccgg gattatccgg
      601 aatgccggac ttgggccatt gctcgagcgc cgcgaccagg gcgtcctggg cgagttcctc
      661 ggcaaggccg atatcgcgga cgatgcgcgt cagtccggcg atgagcttcg cctgctcgat
      721 ccgccagacg gcgtcgatgg cgcggtgggt gtcggtcgcc gccatggcga ccgactatgg
      781 ccacttccac cgtggccgca agccgcgtta ctcggcggcg gcgcgctgct ctgcgccttg
      841 gccgcagact tcctggccgg gatcgggaat ctggcgcacc tcgcactcgc cttcccaacc
      901 aggcatgtgc ttcttgtgca gctccatgaa acgctcggtc catttgcgca tctgcgcctt
      961 cgaggcgcat tcgaagatgg cataaccacc gaccacctcc ttggcctcgg tgaatgggcc
     1021 gtcggtgagg gtgagcttgc cgccggcaag ccgcgcccgc atgccgctcg aagtcggcag
     1081 cagacctacc ccctgcacca tgacgcagcc gtccttggcg gcttcttcca ccatggcatt
     1141 gatcgcgtcc atcatggcct tgggggttgg cgtcatcttc gcttcgttca ttttcaccat
     1201 cgagaggaat cgcatcgtcg tatctcctat gtttgaatcg gcttggacta tttctgcagg
     1261 gccagctcgc ggaagcggtc gagcgcttcg ctctcgccga aatcttcgag ctcgaacaat
     1321 tgccgcacct cgatctcgca gtcacggccg tcgagcgtcg gattggggaa acgccgcgac
     1381 cattcgatcg cctcctcgcg cgacttcacc tggatcatcg tgtagccggc gatcagctcc
     1441 ttggcctcgg tgaaggggcc gtcgacgatg gtgcgcttgc cgcccgaata tttgacccgc
     1501 cagcccttcg aggtcggctg tagcccgctg gcatcgagca gcacgccggc cttggccagc
     1561 ttctcgtgat agtccgccat ggcggcgaac aattcctcgg gcggcttgac gccggcctcg
     1621 gactccttgg tggctttgac cacaatcatg aaacgcatcg tcgctgtctc cttctgttgt
     1681 ggagccccgg cgtccggcga gcggaggtga ggctctaccc acacgtcgaa cggcgcgcgg
     1741 ggaaatcgac atgtcgaaac aatttttttc ggcaatttgg aggtgaagcc ggctaagcct
     1801 tgtcggccaa gcccttgacg atggcggcct cgagcgcggc gatgcgctgc ttgaggagct
     1861 cattctcctc gcgcgccttc tgcgccatgg ccttgaccgc ctcgaaatcc tcgcgctgca
     1921 ccacgtcgag atcgcgcaac acgcgctcgg cctgggcgcg caccacggtt tcgatctcct
     1981 tgcgcacgcc gtcggccgcg ccggcggcac cggtgatcag cttgcccaat tcgtcgaaga
     2041 accggcctgt ggtctgcgtc atgactggac tatgggatgg ccggtctcga ggcgcaagcc
     2101 ggtcctgtca tgagcggccg ttcggcgcct tgacagcagc cgcccgccgg tgaagggtcc
     2161 ggcctcccgc caggacgcaa gccgagcccg catggaactc cttgtcatcc cctacccggt
     2221 gatcgatccc atcgcgatcg aattcgggcc ggttgcggtc cgctggtatg ggctggccta
     2281 tatggccggc atcctgctcg gctggctgta tgggcggcgg ctcgtgtcgc ggcccgatct
     2341 atggggcggc aagccgccgc tgacggtcac gcaagccgac gatttcctgc tctggattac
     2401 cctcggcatc gtggtcggcg gcaggctcgg cttcgtgctg ttctacgagc caagctattt
     2461 ctggcagaac ccggtcgaga tccccgcggt gtggcatggc ggcatgtcgt tccatggcgg
     2521 gctgctcggc gtcgtgctcg cggtctatct gtactcccgc gtccaaggca tcaatccgct
     2581 gtcgcttggc gacatcgcct cggcggcgac gccgttcgga ctcttcttcg ggcggatcgc
     2641 caatttcatc aatgcggaag tggtgggacg ggtgagcgag gtgccctggg cgatggtgtt
     2701 ccccggcgcg ggcgatcagc cgcggcatcc gagccagctc tatgaggcgt ttctcgaagg
     2761 catcgtgctg ttcatcatcc tgcgcatcgc cacccacaag tttcacgcgc tgacgcgacc
     2821 gggcacggtg ttcgggttgt tcctcgtctt ctacggcatc ttccgcagcg cggtcgagtt
     2881 cgtgcgcgag ccgcatgccg gccacccgat ggatgtcggc atcttcaccc ccggcatcgt
     2941 ctattccatt cccatgatcc tcatcggcgc atggctgatc tggcgggcga gacggaagcc
     3001 tgccctggcg tcgtcgtgat gggcgcgcaa cgcaagcctc accgcgccga agagcggccg
     3061 cttgcggcga ggttgcaaca gcttatcgcg cgcaacgggc cgatcagcgt gcatgactat
     3121 atgcaggcct gcctcgccga cgcccgcgcc ggctattacc cgacgcggca gccgatcggc
     3181 aaagacggcg acttcgtcac cgcgcccgag atcagccaga tgttcggcga gctgcttggc
     3241 gtctgggccg tggccgtttg gcaggcgatg ggcgagccgg cggtggtcat cgccgagctt
     3301 gggcccgggc gcggcacgct gatagccgac gcgctgcgcg tgttcggccg cgtgcccgga
     3361 ctgcgcgacc gcgtcaccat cgccctggtc gaaacgagcc caagcttgcg cgagacgcag
     3421 cgcgcgacgt tacgctcctc ccctgccccg ctgctctggt gcgaggacat cggcgaggtg
     3481 ccgcaaggcc cgctcattgt catcgccaac gagttcgtcg acgcgctgcc ca
//
LOCUS       DATNAE010000216         2066 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_1116821, whole genome shotgun sequence.
ACCESSION   DATNAE010000216 DATNAE010000000
VERSION     DATNAE010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1116821"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1316
                     /locus_tag="VN518_04950"
     CDS             <1..1316
                     /locus_tag="VN518_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03955.1"
                     /translation="LKFDHPGLDTAAVRGDHRLLIQNDIGTTDAHVLVVSVEGSAVTV
                     THSDVHEPRAKFFVGVFDAFPVKWSRLRQERAEGLAKGEAFYLISGRYEAGQESLQAF
                     LEAIGAALVFLIDWNKARKALRKLVSNGDAVVLLNWAARHEIGHRGFLQLRGIELVAS
                     AVRQAAPARIGFGEELGDVLGREATLEFLKTALRLSTEALRQGRSARSVRETLEVDLV
                     RRIERNESALLTTVVRQLGLARDIATAMAGDIADGRFGRVDAARHAARAKRIEGKADA
                     IALEARRSIERTQANPTMTELVNAAEDAIDELEQASFLVSLLPRELAENLLQPLSELC
                     DGAISGAEAAVRGLEAAASLSEGNGGEAADSEDALSSTARLVDIEHAADRAERAVTRL
                     VLGGDAAGEGSMVVLELARSLERASDRMALVGHVLHNQMMSGLSH"
     gene            1330..>2066
                     /locus_tag="VN518_04955"
     CDS             1330..>2066
                     /locus_tag="VN518_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HXE03956.1"
                     /translation="MRIVMIKAGGGEGGSPEEIGAKASILARVAELGLPVPPAFVLPI
                     ELGAAMSAGDKEAERTFVAYLKEGIAFLEDASGLRFGHRRKPLLVSVRSGAARSMPGM
                     LDSVLDVGCNSEATRGLIRMTGNPRFAWDCRRRFLEGYAKTVLGLDQSPFSAKRDELT
                     AEEGVANDLELDSEALERLAFIYEALIVSESDEIEDDPMQQLIEAARAVYRSWNGDRA
                     LTYRRLQGFDDLKGSAVMVQAMVFGNSG"
BASE COUNT          351 a          640 c          694 g          381 t
ORIGIN      
        1 gactcaaatt cgatcacccc ggcctcgata ccgctgcggt ccgcggcgac catcgccttc
       61 tgattcagaa cgatatcggc acgaccgatg cccacgtcct cgtggtgagt gttgagggct
      121 cagccgtcac cgtcacacat agcgatgtgc atgagccgcg cgccaagttt ttcgtcggtg
      181 tgttcgacgc atttccggtg aaatggagcc ggctccgcca agaacgggcc gagggcctcg
      241 ccaagggaga agccttctac ctcatcagcg gccgctacga agccggtcag gagtcgcttc
      301 aggcgtttct cgaagcgatc ggcgcggcgc tggtttttct catcgactgg aacaaggcgc
      361 gcaaggcttt gcgcaagctg gtcagcaatg gcgatgccgt tgttctgctg aattgggccg
      421 cgcggcatga gatcggtcac cgtggctttc ttcagctccg cggcatcgag ctggttgctt
      481 ctgccgtgcg acaagctgcg cccgcccgca tcggcttcgg cgaggagctg ggcgacgtgc
      541 tcgggcggga ggcaacactt gaatttctca agaccgcgct caggctgtcg accgaagcct
      601 tgcgccaagg gcgttcggcg cgctcggtgc gcgagactct cgaagtcgac ttggtgcgtc
      661 gcatcgagcg caacgaaagc gctttgctca ccaccgtcgt ccgccagctc ggtctcgctc
      721 gcgatatcgc cacggctatg gccggcgata ttgccgatgg gcgatttggc cgcgttgacg
      781 cggcgcgcca tgcggcgcgc gccaagcgta tcgaggggaa ggccgatgca atcgcgctcg
      841 aggcgcggcg ttcgatcgag cggacgcagg ccaaccccac catgaccgag ctcgtcaatg
      901 cggctgagga tgccatcgac gagctcgagc aagcctcctt tctggtctcg ctcttgccgc
      961 gcgaactggc ggagaatttg ctccagcctc tgagcgaact ttgcgatggc gccatttccg
     1021 gtgccgaggc ggcagtgcgc ggtctggaag cggcagcgag cctgtcggag ggcaatggcg
     1081 gcgaggcggc cgacagcgag gacgcgctct cgtccacggc ccgtcttgtg gatatcgagc
     1141 acgcggccga tcgcgccgaa cgcgcggtca cgcgcctcgt gctgggcggc gatgcggcag
     1201 gcgaggggag catggtcgtg ctcgagcttg cccgctcgct cgagcgggcg agcgaccgca
     1261 tggccctggt tggacatgtc ttgcacaacc agatgatgtc cgggctttcg cactaaaaag
     1321 ggagcgctcg tgagaatcgt catgatcaag gctggaggcg gcgagggcgg aagccccgag
     1381 gagattggcg ccaaggcgtc gatcctcgcg cgcgtcgctg agctcggact gccggtgccg
     1441 ccggccttcg tgttgccgat cgaactcggt gctgccatga gtgcgggcga caaggaggcc
     1501 gagcggacct tcgtcgccta tctcaaagaa gggattgcgt ttctcgaaga cgcctcgggc
     1561 ttgcgcttcg gccatcgccg caagcccttg ctggtgtcgg ttcgctcggg cgccgcgcgc
     1621 tcgatgccgg gcatgctcga cagcgtgctc gacgtgggtt gcaacagcga ggccacgcgc
     1681 ggcctgatcc gcatgacggg caatccgcgt ttcgcctggg attgccgccg ccgcttcctc
     1741 gaaggttatg ccaagaccgt tctgggcctc gaccagtcgc ccttcagcgc gaagcgtgat
     1801 gagttgaccg ccgaagaggg cgtcgccaac gaccttgaac tcgacagcga agcgttagag
     1861 cgcctcgcat tcatttatga agcgctcatc gtgagcgaat ccgacgagat cgaagacgat
     1921 cccatgcagc agctgatcga agcggcccgc gcggtttatc gatcctggaa cggagaccgc
     1981 gctctgacct atcgacgtct gcaaggcttt gacgatctca agggcagcgc cgtcatggtg
     2041 caggccatgg tgttcggcaa tagcgg
//
LOCUS       DATNAE010000217         2804 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_4424632, whole genome shotgun sequence.
ACCESSION   DATNAE010000217 DATNAE010000000
VERSION     DATNAE010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4424632"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="VN518_04960"
     CDS             <1..343
                     /locus_tag="VN518_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03957.1"
                     /translation="WDALTDRERGLIDGGCLATLQATLNSSAKLQADALADFAKAGVR
                     IENFPPGVLASLHQAWADVAKDEGDQDYFFRTVLDDIEKFTAKSQSPSPPAAPNVSAE
                     PSAQAETKATP"
     gene            complement(354..734)
                     /locus_tag="VN518_04965"
     CDS             complement(354..734)
                     /locus_tag="VN518_04965"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HXE03958.1"
                     /translation="MRSFQLIGLSAFALLAVHGPALADGDAAKGKEAFAKCGICHNFQ
                     TNGVGPELKGIVGRKAASAPDYTYSAGMKKLGDDGFVWTPEHIDTWITNPKAMLPDSP
                     MGQLFAGVPDAQERANIIAYLQTQ"
     gene            1026..2462
                     /locus_tag="VN518_04970"
     CDS             1026..2462
                     /locus_tag="VN518_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HXE03959.1"
                     /translation="MSHIALPSPDLAILQRRNDILKQLKKVAPGASLIADQEGRRTFE
                     TDALTAYRCLPLAVVLPANTEEVSAILRYCHENHIKVVPRGAGTSLSGGALPLEDAIV
                     LCVSRMNKLLSVNLTNRVAQVEAGITNSAITEAVASEGYFYAPDPSSQIACTVGGNVA
                     TNSGGAHCLKYGVTTNNVLGVRIVLMTGEIVDIGGPSLDPAGYDFLALIVGSEGQLGV
                     VTEVTVRLLKAAEGARPMLLGFDSNEAAGGCVAAIIGAGIIPVALEFMDRPAIHVCEH
                     FVAAGYPLEVEALLIVEVQGSEDEIDMLLEKIGEIARDFNPTVVRRSGSKEESAKIWK
                     GRKAAFGAIGQLSDYYCMDGVIPLSKLPKALDEIGWICRRYRFDVANIFHAGDGNLHP
                     LILYNANDPLQLERVELCGAEILKLCVELGGCLTGEHGVGIEKRELMRVQFSEADIMQ
                     QMRVKAAFDPEWLLNPGKVFPLEGIGPQ"
     gene            2459..>2804
                     /locus_tag="VN518_04975"
     CDS             2459..>2804
                     /locus_tag="VN518_04975"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HXE03960.1"
                     /translation="MTGHMRVGFHAPDTVEELAYLVAEAADTRTPLEVMGRGTKREVG
                     RPVQYGAVLSTENLTGIELYEPSELVLVARAGTPIGQIEQALTEHDQEMPFEPIDLGP
                     VLGFGRGQGTVGG"
BASE COUNT          487 a          917 c          897 g          503 t
ORIGIN      
        1 ctgggacgcc ctcaccgatc gcgagcgcgg cctgatcgac ggcggatgcc tcgccacgct
       61 ccaggcgacg ctgaacagca gcgccaagct tcaggccgac gcgcttgccg attttgccaa
      121 ggctggggtc cgcatcgaga atttcccgcc gggcgtgctc gcgtccctgc atcaagcttg
      181 ggccgacgtg gccaaggacg aaggcgacca ggactatttc ttcaggaccg ttctcgacga
      241 catcgagaaa ttcaccgcca agtcgcaaag tccctcgccg ccggccgcgc cgaacgtctc
      301 ggctgagccg agtgcccagg ccgagacgaa agccacgccg tagtgcgatt tgtctattgc
      361 gtctgcaggt aggcgatgat gttggcccgc tcctgcgcgt cgggaacgcc cgcgaacaac
      421 tggcccatgg gggaatccgg aagcatcgcc ttggggttgg tgatccaggt gtcgatatgc
      481 tcgggggtcc aaacgaagcc gtcgtcgccg agtttcttca tgcccgccga gtaggtgtag
      541 tccggcgcgc ttgcggcttt acgcccgacg atgcccttga gctccggccc cacgccgttc
      601 gtctggaagt tgtgacagat gccgcacttg gcgaaagcct ccttgccctt ggcggcatcg
      661 ccgtcggcaa gcgccggccc atgcaccgca agcaacgcaa aagcactcag tccaataagc
      721 tggaaactcc tcatctcgat cctccttata tcgtccgtca gagatctcca gcgctctgtt
      781 tggacgatgg cccagcgttc tcccttagtc ttgttgttgt ttggcgactt tagcagaacg
      841 ccgcaagccc tcaaaagcag ggacctgcgg catcgtaact cggaatcggc ccgacagttg
      901 ccttgccagc ccgcgcaatt gcccttcgcg gggcagggct tcttgcccgc cagcccttaa
      961 gcatgcgaaa gactagggct gcggttttcc ttaaaacatc gtttcggacg cgcgcggagc
     1021 ctctgatgtc gcatatcgcc ttgcccagcc cggatctcgc gatcttgcag cgcaggaacg
     1081 acatcctgaa gcaactgaag aaggttgctc ctggcgcgag cttgattgcc gaccaggagg
     1141 gacgtcgcac cttcgagacc gatgcgctca ccgcctatcg ctgcctgccg ctggcggtgg
     1201 tcctgccggc gaacaccgaa gaggtgagcg ccatcctccg ctactgccac gagaaccaca
     1261 tcaaggtcgt gccgcgcggg gccggcacct cgctctccgg tggggcgctg ccgctcgaag
     1321 acgcgatcgt gctgtgtgtc tcgcgcatga acaagctgct gtccgtcaac ctcaccaacc
     1381 gcgtggctca agtcgaggcc ggcatcacca attcggccat caccgaggcg gtcgccagcg
     1441 agggctattt ctatgcgccc gacccctcga gccagatcgc ctgcaccgtc ggcggcaacg
     1501 tcgccaccaa ttccggcggc gcgcattgcc tgaaatacgg cgtcaccacc aacaacgtgc
     1561 tcggcgtccg catcgtcctg atgaccggcg agatcgtcga tatcggcggt ccgagcctcg
     1621 atccggccgg ctacgacttc ctggccttga tcgtcggatc ggaaggccag ctcggcgtcg
     1681 tcaccgaggt gacggtgcgg ctgctcaagg cggcggaggg cgcgcggcct atgctgctcg
     1741 gctttgactc caacgaggcg gccggcggct gcgtcgccgc catcatcggc gccggcatca
     1801 ttcccgtggc gctcgagttc atggatcggc cggcgatcca tgtctgcgag catttcgtcg
     1861 ccgccggcta cccgctcgag gtcgaggcgt tgctgatcgt cgaggtgcag ggctccgagg
     1921 acgagatcga catgctgctg gagaagatcg gcgagatcgc ccgcgacttc aatcccacgg
     1981 tggtgcgccg gagcggcagc aaggaggaga gcgccaagat ctggaagggc cgcaaggccg
     2041 cgttcggcgc catcggccag ctctcggact attactgcat ggacggggtg attccgctgt
     2101 cgaagctgcc caaggcgctc gatgagatcg gctggatctg ccggcgctat cgtttcgatg
     2161 tggccaacat ctttcatgcc ggcgacggca atctgcaccc gctgatcctc tacaacgcca
     2221 acgatccgct gcagctcgag cgggtcgagc tgtgcggcgc cgagatcctg aagctctgcg
     2281 tcgagcttgg cggctgcctc accggcgagc atggcgtcgg catcgagaag cgcgagctga
     2341 tgcgcgtgca gttctccgag gccgatatca tgcagcagat gcgggtcaag gccgccttcg
     2401 atcccgaatg gctgctcaat cccggcaagg tgtttccgct ggaagggatc ggaccgcagt
     2461 gaccggacat atgcgtgtcg gcttccacgc tcccgatacg gtcgaggagc ttgcctatct
     2521 cgtggccgag gccgccgaca cgcgcacgcc gctcgaggtg atggggcgcg gcaccaagcg
     2581 cgaggtgggg cggccggtgc aatatggcgc ggtgctgagc accgagaacc tcaccggcat
     2641 tgagctctat gagccgagcg aactcgtgct ggtggcccgg gccggcacgc cgatcggcca
     2701 gatcgagcag gcgctgaccg agcacgatca ggaaatgccc ttcgagccca tcgatctcgg
     2761 gccggtgctc ggcttcgggc gagggcaggg caccgtcggc ggca
//
LOCUS       DATNAE010000218         6050 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_513962, whole genome shotgun sequence.
ACCESSION   DATNAE010000218 DATNAE010000000
VERSION     DATNAE010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6050
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513962"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="VN518_04980"
     CDS             <1..569
                     /locus_tag="VN518_04980"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the formation of L-threonine from
                     O-phospho-L-homoserine; Derived by automated computational
                     analysis using gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HXE03961.1"
                     /translation="TGIYEPRGVIATSSPSMDIQLASNFERLLFELVGHEGARVRARM
                     DELRAKGAFHLEEGELAEMRTLFAAHSIGEHETEMTIRGLFAETGVMIDPHTAVGLAA
                     ARAERVDTPMIVLSTAHAAKFPQAIERALGRLPEQPERLKAKLGQEERLTVLANDYAS
                     IAQFIESHARAKTDGRKAASKARTGVGA"
     gene            608..1846
                     /locus_tag="VN518_04985"
     CDS             608..1846
                     /locus_tag="VN518_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HXE03962.1"
                     /translation="MSHARADVETVSLGLWVGAGSRSESPPEHGVAHFLEHMAFKGTD
                     RRSAQAIAEEIEAVGGELNAATGVDSTAYYARVLRQDFGLALDILSDIILTPRFDHTE
                     LARERDVILQEIASAQDSPEDMVFDLAQATAFPDQPAGRPILGTAKSVKGFKSTHLKT
                     YLATHYQAPNMVLAAAGAVDHERLLAEAERLLAQLNSGTAPEPTPALYRGGVARNEKS
                     FEQTHLVLAFEAPPYRHADYFAAQVCAGALGGGMSSRLFQEVRERRGLCYAIHAFASG
                     LSDTGMFAIYAACGPDRAAELLAVLRDELVRAAESGFSEEEVGRVKAQLKMGLLAGLE
                     SSGARAEQLARQILIHGRPLTTNELIEKVEAVTLPDVQRIAGSLLASPVSLATVGPIL
                     HVARFDSLAAKFTHVSSEAA"
     gene            1863..2450
                     /locus_tag="VN518_04990"
     CDS             1863..2450
                     /locus_tag="VN518_04990"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HXE03963.1"
                     /translation="MALLRTATSSDLGPVIQSDRVLLRTPQMSDYPDWAELRAASCEF
                     LTPWEPLWAADELTRSSFRRRVRHYLRDLREDMGYALFIFSAANGALVGGLTLCNVRR
                     GVTQACTLGYWIGAPHAQKGYMTAAVRAVIPFVFDSLELHRLEAACLPANVASIKLLE
                     RTGFTREGMARRYLKINGVWQDHLLYALLESDRRR"
     gene            2470..5547
                     /locus_tag="VN518_04995"
     CDS             2470..5547
                     /locus_tag="VN518_04995"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
                     /protein_id="HXE03964.1"
                     /translation="MTDGKVTGAGHEVRGGGLGGRLACLVVLLLAFGLSLMPLPREVA
                     LAVEAIVVKPEQEKLDVTALGELYEGRGDKLQIETAPGQDGYIGRMAVQATTAGTNPG
                     WLVFALTNPTTERITRWLAAPRYTLANSRVLSPDLDAGQIAAITPSLGFRPERMKSDR
                     ADMFRLSLEPGQTITFAAELSSRQVPRLMLWDPEVYQAKQQDRMLFNGVLLGITGLLA
                     IFLTAIFAANHRAIFPATALVAWAALAYFCVDFGFWSKLFPVNADHAAVYRAAAEAAL
                     AASFVLFLYAFLRIGMWHGWIKTLFWCWIAAQFGLVLFAVVDPRLASGLARSSFLGIA
                     AIGTCLIAYLALRGQDRALLLIPSWMLLLVWLFGAAMIVLGKLSGDVVVTGLAAGLVL
                     ILVLLGFTVTQFAFRSTGGGPLQLGPISQVQMKSLAVDGSGAAVWQWNAAKDQITVAQ
                     EIEEALGAPPGTLDTTVDRFLQHMHAADRERFRLMLWSLQENQGGELQTEFRLKRQDG
                     SYLWYELRARAAPSDNTRLLRCVGLMRDVTSLKRSHERLMHDAVHDSLTSLPNRELFL
                     DRLQGAITRAREGQMNRPTVMFIDIDRFKNVNKSFGLAVGDSMLLTLGRRLSRHLNPQ
                     DTLARLGSDQFAILMISETDPRQVATLAERVRRALRTPMKIGGKEIVLTASIGIVVDD
                     GVQPTAQDLLREGEIAMLRAKRAGADRIEIFTASLRGEDDSRLPLESELRKALEKRQI
                     AVLYQPITRLASNQLAGFEALIRWDHPTRGQLSPDEFVPIAEETGLIGELGSYVLGQA
                     LEEARGWHKVLPRERDPLFVSVNVSSRQLFRQDLVQEIRLILAKEAVPKGTLKLEITE
                     SLVMENPERAVEILTWLKSFGASLALDDFGTGYCSLSYLHRFPCDTIKVDRSLVRDSG
                     LNGSTPMILRSIVALSHELGKEVIAEGVETAEDAAYLRSIGCEFGQGFYYGEPMTTKE
                     VADLLSALASHRKRQQRERSKAAARGHAAPAPVARPVAQTAPPRPKPAASRVS"
     gene            complement(5533..>6050)
                     /locus_tag="VN518_05000"
     CDS             complement(5533..>6050)
                     /locus_tag="VN518_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HXE03965.1"
                     /translation="RAVIYMCAHSADGAMGIIINHRAPNISFTELLEQLNIVPPKERI
                     RLPMGLGAMAVHLGGPVETGRGFVLHSADYFKAESTLPIDESVCLTATIDILRDIAKG
                     TGPDKALLALGYAGWAPGQLESEIQANGWLHCAADPELIFDPEVDRKYNRALGKIGID
                     PWRLVNDSGHA"
BASE COUNT          981 a         2057 c         1941 g         1071 t
ORIGIN      
        1 cgacggggat ttacgagcca aggggcgtga tcgccacctc cagcccgagc atggatattc
       61 agcttgcgag caatttcgag cggcttctgt tcgagctcgt gggccatgag ggtgcccggg
      121 tcagggcgcg catggacgag cttcgcgcca agggcgcgtt ccatctcgag gagggcgagc
      181 tcgccgagat gcgcactctc ttcgccgccc attcgatcgg tgagcatgag accgagatga
      241 ccattcgtgg ccttttcgcc gaaaccggcg tgatgatcga cccgcatacc gccgtggggc
      301 tagctgccgc gcgggcggag cgtgtcgaca cgccgatgat cgtgttgtcg acggcgcacg
      361 ccgcgaagtt cccgcaagcc atcgagcggg ctctcggccg tctgcctgag cagccagagc
      421 ggctcaaggc aaagctcggc caggaggagc gcctgaccgt gcttgccaac gactatgcct
      481 ccattgccca attcatcgag agccacgcgc gcgccaagac tgacggccgc aaggccgcga
      541 gcaaggctcg gaccggggtc ggcgcgtgag tgtcgtcgta tccagactga ggaatggcct
      601 caccgtagtg agccatgcaa gggccgatgt ggagaccgta tcgctcggcc tctgggtcgg
      661 cgccggcagc cgcagcgaga gcccgccgga gcacggcgtc gcgcattttc tcgagcatat
      721 ggccttcaag ggcaccgacc ggcgcagcgc gcaagccatc gccgaggaga tcgaggcggt
      781 cggcggcgag ctcaacgccg ccaccggcgt cgactcgacc gcctattacg cgcgggtgct
      841 gcgccaggat ttcggcctcg cgctggatat cttgagcgac atcattctta ccccgcgctt
      901 cgaccatacc gagcttgccc gcgagcgcga cgtgatcctg caggagatcg cctctgccca
      961 ggattcgcct gaggacatgg tattcgacct cgcgcaggcc actgcctttc ccgatcagcc
     1021 tgcggggagg ccgatcctcg gcaccgcgaa gagcgtcaag ggattcaagt ccacccatct
     1081 caagacctat ctcgccaccc actatcaagc gccgaacatg gtgctcgctg ccgcaggtgc
     1141 cgtcgatcac gagaggctgc ttgccgaggc cgagcgcctg ctcgcgcaac tcaattcggg
     1201 cacggcgcca gagcccacgc ctgcgcttta ccgcggcggc gtcgcgcgca acgagaagtc
     1261 gttcgagcag acccatctgg tgcttgcctt cgaggcgcct ccttaccgcc atgccgatta
     1321 tttcgccgcg caagtctgcg ccggcgcgct tggcggtggc atgtcctcgc gcttgttcca
     1381 ggaggtgcgc gagcgccgcg ggctctgtta cgcgattcac gctttcgcct ccggactttc
     1441 cgataccggc atgttcgcga tctacgccgc ttgcggaccc gaccgcgccg ccgagcttct
     1501 cgccgtgctg cgcgacgagc tcgtccgcgc cgccgagagc ggcttcagcg aggaggaagt
     1561 ggggcgggtc aaggcccagc tcaagatggg gcttcttgcc gggctggaga gcagcggtgc
     1621 ccgcgccgag cagctcgccc gccagattct cattcacggg cggccgctta ccacgaatga
     1681 gctgatcgaa aaggtcgagg ccgtgacgct tcccgacgtg caaaggatag ccgggagcct
     1741 gcttgcctcg ccggtcagcc tcgccaccgt cgggccaatt cttcatgttg caagattcga
     1801 tagcctcgcg gcaaaattca cccatgtctc gagcgaagcg gcctgaggag cattgagcga
     1861 ccatggcgtt gctgcgtacc gccacctcca gcgatttggg gccggtcatc caatcggatc
     1921 gcgtgctcct gcgtacgccg caaatgtccg actaccccga ctgggccgag cttcgcgccg
     1981 cgagctgcga gttcctcacg ccttgggagc cgctctgggc cgccgacgag ctcacccgct
     2041 cctccttccg ccgccgcgtc cgccattatc tgcgcgacct tcgcgaggac atgggctatg
     2101 cgctgttcat cttctcggcg gcgaacggcg cgctggtcgg cgggctcacc ctttgcaacg
     2161 tgcgccgtgg ggtgacccag gcctgcacgc tcggctactg gatcggcgcc cctcatgccc
     2221 aaaaggggta tatgacggcc gccgttcgcg ctgttatccc tttcgtgttc gattcgctcg
     2281 agctgcaccg gctcgaagcg gcctgcctgc ccgccaatgt cgcctccatc aagcttctgg
     2341 agaggaccgg cttcacgcgc gaggggatgg cgcgccgcta cctgaagatc aacggcgtgt
     2401 ggcaggacca tctgctgtac gcgctgcttg aaagcgacag acgccgttag gagccgattg
     2461 ggggcgccat tgacagacgg caaggtgacg ggagctggcc acgaggtgag gggaggaggc
     2521 ttgggcggcc gcctcgcgtg tctcgtcgtg ctgctgctcg cgttcggctt gagcctcatg
     2581 cccttgccgc gcgaggttgc tctcgccgtc gaggccatcg tggtcaagcc ggagcaggag
     2641 aagctcgacg tcacggccct tggcgagctc tatgaaggac gcggcgacaa gctgcagatc
     2701 gagaccgcac ccggccagga tggctatatc ggccgcatgg cggtgcaggc gaccaccgcc
     2761 ggcaccaatc caggctggct cgtctttgct ctgaccaacc ccaccaccga gcgcatcaca
     2821 cgatggctcg ccgcgccgcg ctacacgctt gccaattcgc gcgtgctctc gcccgatctc
     2881 gatgccggcc agatcgccgc catcacgccg tcgcttggct tcaggcctga gcgcatgaag
     2941 agcgaccgcg ccgacatgtt tcgcttgagc ctcgagccag ggcagaccat caccttcgcc
     3001 gccgagttga gctcgcgcca ggtgccccgg ctcatgctgt gggatcccga ggtctatcag
     3061 gcgaagcagc aggaccgcat gctgttcaac ggcgtgctgc tcggcatcac cgggctgctc
     3121 gccattttcc tcacggcaat cttcgccgcc aatcaccggg ctatcttccc ggccaccgcg
     3181 ctcgtggcct gggcggctct cgcctatttc tgcgtggatt tcggcttttg gagcaagctg
     3241 ttcccggtca atgccgatca tgctgccgtc taccgcgccg cggccgaagc ggcgttggcg
     3301 gcgagcttcg tgctgttcct ctatgccttc ctgcgcatcg gcatgtggca cggctggatc
     3361 aagaccctgt tctggtgctg gatcgccgcc cagttcggcc tcgtcctctt cgccgtcgtc
     3421 gatccgcggc tcgcctcggg ccttgcccgc agctcgttcc tcggcatcgc cgccatcggc
     3481 acctgcctca tcgcctatct tgcgctgcgc ggccaggacc gcgcgctcct gctcattccg
     3541 agctggatgc tcttgctcgt ctggctgttc ggcgccgcca tgatcgtgct cggcaagctg
     3601 tcaggcgacg tcgtggtgac aggtcttgcc gccggcctcg tccttatcct ggtgctgctc
     3661 ggcttcaccg tgacgcaatt cgccttccgc tccaccggcg gcggcccgct ccagctcggg
     3721 ccgatcagcc aggtgcagat gaagtcgctc gccgtcgatg gctctggcgc cgccgtctgg
     3781 caatggaacg ccgccaagga tcagatcacc gtcgcccagg agatcgagga agcgctcggc
     3841 gcgcccccag gcaccctcga taccaccgtc gaccgcttcc tccagcacat gcatgccgcc
     3901 gaccgcgagc gcttccgcct gatgctgtgg tcgctgcagg aaaaccaggg cggcgagctg
     3961 caaaccgagt tccgcttgaa gcgccaggac ggcagctatc tgtggtacga gctccgcgcc
     4021 cgcgccgcgc cgagcgacaa tacccggctc ttgcgttgcg tcggactgat gcgcgacgtc
     4081 accagcctca agcgctcgca tgagcggctg atgcacgacg ccgtgcatga cagcctgacc
     4141 agcttgccca accgcgagct gttcctcgat cgcctgcaag gcgctatcac ccgcgcccgc
     4201 gaagggcaga tgaaccgccc gacggtgatg ttcatcgaca tcgaccgctt caagaacgtc
     4261 aataagagct tcgggcttgc cgtcggcgac agcatgctgc tgaccctcgg gcgcaggctc
     4321 tcccgtcatc tcaatccgca agacacgctc gcccgtctcg gcagcgacca gttcgccatt
     4381 ctgatgatct cggagaccga tccgcgccag gtggccaccc ttgccgagcg cgtgcgccgg
     4441 gcgctgcgca cgccgatgaa gatcggcggc aaggagatcg tgctcaccgc ctcgatcggc
     4501 atcgtggtcg atgacggggt gcagccgacg gcgcaagatc tgttgcgcga gggcgagatc
     4561 gccatgctca gggccaagcg cgccggcgcc gaccgcatcg aaatcttcac cgcctcgctg
     4621 cgcggcgagg acgacagcag gctgccactc gaaagcgagc tccgcaaggc gctcgagaag
     4681 cggcagatcg cggtgctcta ccagcctatc acccgccttg cctcgaacca gctcgcaggc
     4741 ttcgaggcgc tgatccgttg ggaccatccg acgcgcggcc agctcagccc ggacgagttc
     4801 gtgcccatcg ccgaggagac cgggctgatc ggcgagctcg gcagctatgt tctcggccag
     4861 gccttggagg aagcgagagg ctggcacaag gtgctgccgc gcgagcgcga cccgctcttc
     4921 gtcagcgtca atgtgtcgag ccgccagctc ttccgccagg atctcgtgca ggagatcagg
     4981 ttgattctcg ccaaggaggc ggtgcccaag gggacgctga agctcgaaat cactgagtcg
     5041 ctggtcatgg agaaccccga gcgcgccgtg gaaatcctca cctggctcaa gagtttcggc
     5101 gcgagcctgg cgctcgacga tttcggcacc ggctattgct cgctctctta tctgcatcgc
     5161 ttcccttgcg acaccatcaa ggtcgaccgc tcgctcgtgc gcgattcagg gctcaacggc
     5221 tcgacgccaa tgatcctgcg ctcgatcgtg gcgctctctc acgagctcgg caaagaggtg
     5281 atcgccgagg gcgtggagac cgcggaggac gcagcctatc tccgctcgat cggctgcgaa
     5341 ttcggccaag gcttctatta cggggagccg atgacgacca aggaggtggc ggacctgttg
     5401 tcggcactcg cgagccaccg caagcgccag cagcgcgaga ggtcgaaggc ggcggcgagg
     5461 ggccatgcag caccggcgcc ggtggcgaga ccggtggcgc aaacagcgcc gccgcggccg
     5521 aagcctgcgg cctcacgcgt gtcctgaatc gttgaccagc cgccagggat cgatgccgat
     5581 cttgccgagc gcgcgattat atttgcggtc gacttccgga tcgaaaatca gctctgggtc
     5641 ggccgcgcaa tgcagccagc cattggcttg gatctcgctc tcgagctgcc cgggcgccca
     5701 cccggcatag ccgagcgcga gcagcgcctt gtcggggccg gtgcccttgg cgatgtcgcg
     5761 caagatgtcg atggtggcgg tgaggcacac gctctcgtcg atcggcagcg tgctctcggc
     5821 cttgaaatag tcggcgctgt gcaggacgaa gccgcggccg gtctccaccg ggcctccgag
     5881 atgcaccgcc atggcgccaa gccccatagg cagtctgatg cgctccttcg gcggcacgat
     5941 gttgagctgc tcgagcagct cagtgaagct gatatttggc gcgcggtgat tgatgatgat
     6001 gcccatggcg ccgtcggcag agtgagcgca catatagatc acggcgcggg
//
LOCUS       DATNAE010000219         4203 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_516347, whole genome shotgun sequence.
ACCESSION   DATNAE010000219 DATNAE010000000
VERSION     DATNAE010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516347"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..848)
                     /locus_tag="VN518_05005"
                     /pseudo
     CDS             complement(112..848)
                     /locus_tag="VN518_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012091020.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
     gene            complement(956..1303)
                     /locus_tag="VN518_05010"
     CDS             complement(956..1303)
                     /locus_tag="VN518_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03966.1"
                     /translation="MVAAFVANLPALREQWRTDRAGSMKTIWMFGVYLLYIGLGIVVV
                     LLLAPKAGGAGGDKALLLTGFILGFIFYGALTFMRVVPRYREPPAWLMRFGIADIVLL
                     GATFACLAGYLWG"
     gene            complement(1336..1863)
                     /locus_tag="VN518_05015"
     CDS             complement(1336..1863)
                     /locus_tag="VN518_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03967.1"
                     /translation="MTGKSKSFGEVFDAHTAAEFQTRDIEATMGTMGGAPHVTHVPVM
                     TGGHGADAVRSFYETWFIGHWPEDIKITLISRTAGESQLVDELILSFTHDCDMPAILP
                     GLKPTGRKVTVPTVVIVGFDQDNKITHEHIYWDQATMLVQLGLLDKVALPVTGAEQAA
                     RLLDPKLPSNTLIPK"
     gene            complement(1860..2816)
                     /gene="gshB"
                     /locus_tag="VN518_05020"
     CDS             complement(1860..2816)
                     /gene="gshB"
                     /locus_tag="VN518_05020"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HXE03968.1"
                     /translation="MALKVAFQMDPIGRIDIRGDSTFALLLEAQWRGHDVFYYTPNNL
                     SLRDGRLIALGHSLTVEDKPGDHYRLAHQREEDLSKFDVVHLRQDPPFDMAYITTTHL
                     LERIHPKTLVVNNPAAVRNAPEKVFVLDFLDLMPPTLVTRSPDAIRAFRDKHKDIVVK
                     PLYGNGGAAVFHVPPGDTNLNSLIELLGMTFREPIMVQRYLPEVRGGDKRIILVDGEI
                     AGAINRVPAPDETRSNLHVGGRAEPATLTPRDREICARLGPELKKRGLIFVGIDVIGD
                     YLTEINVTSPTGIRQVAQFGGPDIAAMIWDVIEVKVKREKKR"
     gene            complement(2875..3234)
                     /locus_tag="VN518_05025"
     CDS             complement(2875..3234)
                     /locus_tag="VN518_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HXE03969.1"
                     /translation="MSEKRVRAYRHGLLAETIAALLLRLKGHRIIARRYKTPVGEIDL
                     VALKSKRLAFVEVKQRKTFDEAGWSLPSRARRRIVRAAQYWLAGHPDFAGHDIAFDVV
                     LAAPWALPRYIENAFPV"
     gene            complement(3231..4166)
                     /gene="rsmI"
                     /locus_tag="VN518_05030"
     CDS             complement(3231..4166)
                     /gene="rsmI"
                     /locus_tag="VN518_05030"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HXE03970.1"
                     /translation="MQDHRHDDQDAARKLIQRAEAALAGEMERQLQPGLYLVATPIGN
                     LADISLRALAVLNRADLIAAEDTRHSRKLLSHFGIKAELTPYHEHNAAKERPRLLARI
                     RAGFAVALISDAGTPLISDPGYKLVREALEAGLQVTSVPGPSAALAALTSAGLPTDTF
                     LFAGFLPPKGGARRKRLEELKPVPATLIFYETASRLHAALADMAEVYRAREAAIAKEL
                     TKLHESVARGTPAALAAAFDSTELKGEFVVLVGPPNPDEAEIGDEAIVARLKQALKQE
                     SFRDAVRSVAEDFKVKRSRVYELGLALERKGSNRP"
BASE COUNT          722 a         1325 c         1421 g          735 t
ORIGIN      
        1 ttcgaaccct gtgtgacagt ttggggatat tggggcgcgg cccggctttt cacccaggat
       61 aatgtttatc cagagcttgg ccaggactca cgcggcggct tgcgcgcggc gcgcatcgag
      121 cacttgcgcg gcgaactcag gaagctcgtc gaggaatagc acgccgagat gcgccaaggc
      181 cacctcgccc gggcgcgggc gcgtgccgcc gccgacgagc gccgccatgc tcgccgaatg
      241 atgcggggcg cggaagggac gcgtcctgtt gatgtcgcgc aagtcgggca tcgcctcgtc
      301 gcctgggtcg ggcgtcggca gcgggcgctt cagcgtctgc aggcctttga agtggttgac
      361 gagctgaagc agattgcacg gcgcgaggat ctgcatgtcg gcagcagccc acgcagcctc
      421 ggcgcctgag tcgcgcgggc agatcagccc tttgccaagc gcgttggccc caatcgcggc
      481 gggaagagcg ccggcgatcg ccgcaatcga gccgtcaagg gcaagctcgc cgagcacgca
      541 ataggcttcg atcgcgtcat gcgccacggc gccgctcgca gccatcagcc cgagcgcgat
      601 ggggaggtcg tagtggctgc cttccttcgg ctgatcggcg ggggcgaggt tgacggtggt
      661 gcgcttcggc gggagcgcga gcccgatggc gttcagcgcg ccgcgcaccc tctcgcggct
      721 ctcggccacg gcctttcggg gaggccgacg atggtgaagt ggggcatccc cggcccgatc
      781 tgcacccgca catcgatcgg ccgcgcctcg atgccctcaa aggcgacggt ggtgacccgg
      841 gcaaacatcg ctgaagcccc cgttccggct caactctaca gcctcaagtt ccactctagc
      901 caagttgcac gcgagggccg agaacattca gcgaacatcc ggagaggagg agactttatc
      961 cccacaggta accagcgagg caggcgaagg tggctccgag caggacgatg tcggcaatgc
     1021 cgaagcgcat aagccaagcc ggaggctcgc ggtagcgcgg caccacgcgc ataaaggtca
     1081 gcgcgccgta gaagataaag cccaagataa atccagtgag gagcaacgcc ttgtcgccac
     1141 ccgccccgcc ggcctttggc gcgagaagca gcaccacgac gatgccgagc ccgatataga
     1201 gaagatagac gccgaacatc cagatcgtct tcatcgagcc ggcgcggtcg gtacgccatt
     1261 gctcgcggag cgccggcagg ttggcgacga aggcagcgac cacgacgaga gcccaggcct
     1321 tccatgccgc gctcgctatt tcgggatcag cgtgttggaa gggagcttcg gatcgagcag
     1381 ccgcgccgcc tgctcggcgc cggtcaccgg gagcgcgacc ttgtccagga gcccgagctg
     1441 caccagcatg gtcgcctggt cccagtagat gtgctcatgc gtgattttgt tgtcttgatc
     1501 gaagccgacg atcaccacgg tgggaaccgt caccttgcgc ccggttggtt tgaggcccgg
     1561 cagaatcgcc ggcatgtcgc aatcgtgcgt gaaggataga atcagctcgt cgaccagctg
     1621 gctctcgccg gccgtgcgcg agatcagcgt gatcttgatg tcttccggcc aatggccgat
     1681 gaaccaggtc tcatagaagc tgcgcaccgc gtccgccccg tggccgccgg tcatcaccgg
     1741 gacgtgggtg acatgcggcg cgccgcccat ggtgcccatg gtcgcctcga tatcgcgcgt
     1801 ttggaactcg gctgcggtat gggcgtcgaa cacctcgccg aaggatttgc tcttccccgt
     1861 catcgcttct tctcgcgctt caccttcacc tcgatcacgt cccagatcat cgccgcgatg
     1921 tcggggccgc cgaactgcgc aacctggcgg atgccggtcg gcgaggtgac gttgatctcg
     1981 gtgaggtagt cgccgatcac gtcgatgccg acgaagatca gcccgcgctt tttcagctcg
     2041 ggcccgagcc gcgcgcagat ttccctgtcg cgtggcgtga gcgtggcggg ctctgccctg
     2101 ccgccgacat gcagattgga gcgcgtctcg tcgggggcgg gcacgcggtt gatggcaccg
     2161 gcgatctcgc cgtcgaccag gatgatgcgc ttgtcgccgc cgcgcacttc gggaagataa
     2221 cgctgcacca tgatcggctc gcggaaggtc atgccaagaa gctcgatcag cgaattgagg
     2281 ttggtgtcgc ccggcggaac gtggaacacg gcggcgccgc cattgccgta gagcggcttc
     2341 accacgatgt ccttatgctt gtcgcggaac gccctgatgg cgtctggcga gcgggtgacg
     2401 agggtgggcg gcatcagatc gagaaagtcg agcacgaaca ctttctccgg cgcgttgcgc
     2461 acggcggcag ggttgttcac caccagcgtc ttggggtgga tgcgctcgag cagatgcgtg
     2521 gtggtgatat aggccatgtc gaatggcggg tcctggcgga gatgcaccac gtcgaacttc
     2581 gacagatcct cctcgcgctg atgcgccagc cggtaatggt cgccgggctt gtcctcaacc
     2641 gtgaggctat gtccgagcgc gatcagtctg ccgtcgcgga gcgagagatt gttcggcgtg
     2701 taatagaaca cgtcgtggcc gcgccattgc gcttcgagca ggagggcgaa ggtggaatcg
     2761 cccctgatat cgatgcgccc gatcgggtcc atctggaagg cgactttgag ggccatggtg
     2821 catccttata ggcaaagtct aagcaaagtc tccacccccc ttgcggccag ggaattagac
     2881 cgggaacgcg ttctcgatat atcggggcag ggcccaaggc gcggccagca ccacgtcgaa
     2941 ggcgatgtcg tggccggcaa agtccggatg tccggcgagc caatattgcg cggccctgac
     3001 gatgcgccgc cgcgcgcggc taggcagcga ccagcctgcc tcatcgaagg tcttgcgctg
     3061 cttcacctcg acgaaggcga gccgcttgct tttgagcgcc acgagatcga tctcgccgac
     3121 aggcgtcttg tagcgccgcg cgatgatgcg gtggcctttg aggcgaagca ggagcgcggc
     3181 gatggtttcg gcgagcagcc catgacggta tgccctgacc cgcttctcgc tcacggccga
     3241 ttgctgcctt tgcgctccag cgctaggcca agctcgtaga ctcggctccg cttgaccttg
     3301 aagtcttccg cgacgctgcg caccgcgtcg cgaaagctct cctgcttcag tgcctgcttg
     3361 agtcgcgcca cgatcgcctc gtcgccgatt tccgcttcat cgggatttgg cggaccgacc
     3421 agcaccacga actccccctt gagttcggtc gagtcgaacg ccgcggcaag cgcggcggga
     3481 gtcccgcggg cgacgctctc atgcagcttg gtgagctcct tggcgatcgc agcctcgcgc
     3541 gcgcggtaga cctcagccat gtcggcgaga gccgcgtgaa gccgcgacgc ggtctcgtag
     3601 aagatcagcg tggcaggaac gggcttcagc tcttcgagcc gcttccgccg cgcgccaccc
     3661 ttcggcggca gaaagcctgc aaacaggaat gtgtcggtcg gcaagccggc gctggtcagc
     3721 gcggcgagcg cagccgaagg gcctggcacg ctcgtcacct gaagcccagc ctcaagagct
     3781 tcgcgcacga gcttgtagcc aggatctgag atcagcggcg tgccggcatc cgagatcagc
     3841 gccacggcga atcctgccct gatgcgcgcg agcagtcgcg ggcgctcctt agcagcgttg
     3901 tgctcgtgat agggggtgag ctcagccttg atgccgaaat gggagaggag cttgcggctg
     3961 tggcgcgtgt cttccgccgc gatcagatcg gcgcggttga gaactgcgag cgcgcggagc
     4021 gaaatgtcgg cgaggttgcc gatcggcgtc gcgacaagat agaggcccgg ctgaagctgc
     4081 cgttccatct cgccggcgag cgcggcttca gcgcgctgaa tcagcttgcg cgctgcgtct
     4141 tggtcgtcgt ggcgatgatc ctgcacgcat gcgcttatag ctcaggcagg ctgccggtga
     4201 act
//
LOCUS       DATNAE010000220         4172 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_4122270, whole genome shotgun sequence.
ACCESSION   DATNAE010000220 DATNAE010000000
VERSION     DATNAE010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4172
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4122270"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1305)
                     /locus_tag="VN518_05035"
     CDS             complement(<1..1305)
                     /locus_tag="VN518_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03971.1"
                     /translation="MVALLAADILAQQLGFEAPDRPDGSAALEQRIATLESNRSQRSV
                     ASDVTTRLAAAEAKLGQLEQLNANVDGIAKKQGQLDETLNAVNDKLQADSGDKGAPAR
                     VAKLEEQLSLMSSAAERDPQSGRLPQLTAITGKLTDLELTMANQLDALRKSVALEIDT
                     RLSSATEAGQAARSGTQRMDRELSTLKAESAEMSTGLNAVKTDGDRAAAALKATQEDL
                     KRLKADLDARLATFAKPEDVSSAVNPISGKLAALQQDVQGVIKSEGDRKTTAERIVLS
                     LELANLKRAIDRGNAYAPELEQARKVAGSNVDLAPLERFALDGVPTPTELRAQFKPVA
                     FKIIDAEEQPSEASIVDRLLAGAKSVVRVRKTSHSPDDKSIEAVVARMEIALNEDRLA
                     DVLQESKSLPAPAQDVAQDFLAKVQARDAVDRALASVETQLKA"
     gene            complement(1777..2514)
                     /locus_tag="VN518_05040"
     CDS             complement(1777..2514)
                     /locus_tag="VN518_05040"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HXE03972.1"
                     /translation="MHVLLTRPEPDASTCAAQLEALGHAVTLEPLLRIERLPIDATAL
                     DGVAGVIVTSRNGLRALASSSAFNAALRLPIMAVGPGTAQLARELGFAQVVAGRGTAS
                     ELVPLIVEAGRGQQGAFVHVRGEDAAFDLRAALVAHAIELREIVSYRAVPAHAFQRRT
                     KELMVTGAIDAAIFMSPRTGSVFTLLVQAQGLEQAAKTIVLLCLSPAVAATVEPLVPA
                     RVEVAESPNSAAMLAAVTRVATLWSGV"
     gene            complement(2514..3443)
                     /gene="hemC"
                     /locus_tag="VN518_05045"
     CDS             complement(2514..3443)
                     /gene="hemC"
                     /locus_tag="VN518_05045"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008551662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HXE03973.1"
                     /translation="MQAAKIRIGTRGSPLALAQARQVATRLGAAHGLSPEQCELVIIR
                     TTGDKITDRPLIEAGGKGLFTKELEEALFAGDIDLAVHSMKDMPAILPQGLAISAILK
                     REDPRDAFVSITYASFDELPSGAVVGTSSPRRQAQALRLRPDLRIVPFRGNVETRLRK
                     LEEGVADATFLACAGLNRLGLSQHITAAMNTEVMLPAVAQGAIGIEIRADDDATVRLV
                     APLNDAATALCVTAERAYLAHLEGSCRTPIAGLAELSGGSLHIRGEILTPNGLQAHSA
                     SRRGSAQEAIRLGEQTAEELLRHAGPEFFLATA"
     gene            3553..>4172
                     /locus_tag="VN518_05050"
     CDS             3553..>4172
                     /locus_tag="VN518_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014132365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HXE03974.1"
                     /translation="MANPEQTGGKPSLVLGLETSCDETAAAVVLREPDGSGRILSNIV
                     RSQWERHRAYGGVVPEIAARAHVECLDVLVREAMREADAGFGDLAGVAATTGPGLIGG
                     LIVGAITGKAIALARGLPFVAVHHLEAHALTVGLTEGVAPPYLLLLVSGGHTQIVLVR
                     GVGDYQRWATTIDDALGEAFDKTAKMLGLPYPGGPNVEDAALSGDAA"
BASE COUNT          655 a         1304 c         1455 g          758 t
ORIGIN      
        1 tgccttgagc tgggtttcga cggaggcaag agcgcggtcg acggcgtcgc gcgcctgcac
       61 cttggcgagg aagtcttgcg cgacatcctg cgccggagcg gggagcgatt tcgactcttg
      121 cagaacgtcg gcgaggcgat cttcgttgag cgcgatttcc atgcgcgcga ccaccgcctc
      181 gatgctcttg tcgtcggggc tgtggctcgt cttgcgcacg cgcacgaccg acttggcgcc
      241 ggctagcagg cgatcgacga tcgatgcttc ggacggctgc tcctccgcgt cgatgatctt
      301 gaaggcgacg ggcttaaatt gcgcgcgcag ctcggtcggg gtcggcacgc cgtcgagagc
      361 aaaacgctcg agcggtgcga gatcgacgtt gctgccggca actttccgcg cctgctccag
      421 ctcgggtgcg taggcgttgc cgcgatcgat ggcgcgtttc aggttggcga gctccagcga
      481 gagaacgatg cgctcggcgg tggttttgcg atcgccctcg ctcttgatga cgccctgcac
      541 gtcctgctgc agcgccgcca gcttaccgct gatgggattg accgcggagg agacgtcctc
      601 gggcttggca aaggtggcga ggcgcgcgtc gagatcggcc ttcagccgct tgagatcctc
      661 ttgcgtggcc ttgagtgcgg cagccgcccg gtcaccatcg gttttcacgg cgttgagccc
      721 ggtcgacatc tcggcgctct cggccttcaa tgtggagagc tcccggtcca tgcgctgcgt
      781 gccagagcgc gcggcttgtc ccgcttccgt cgccgagctg agccgcgtat cgatctccag
      841 ggcgacgctc ttgcgcaggg cgtcgagctg attagccatc gtcaattcga gatcggtgag
      901 cttgccggtg atggcagtga gctgcggaag gcgccccgat tgcgggtcgc gctcggcagc
      961 agaggacatc agcgagagct gctcctcgag cttggcaaca cgcgcgggcg cgcccttatc
     1021 accgctgtcg gcttgcagct tgtcgttgac ggcattcagg gtttcgtcga gctggccctg
     1081 cttcttggcg atgccgtcga cgttggcatt gagttgctcg agctggccga gctttgcctc
     1141 ggcggcggcg aggcgagtgg tgacgtcgga ggcaaccgag cgttggctgc ggttggattc
     1201 gagcgtggcg atgcgctgct ccaatgcggc cgaaccatct ggacggtcgg gcgcctcaaa
     1261 tccaagctgc tgggcgagga tgtcggccgc cagcaatgcg accatgccgc cggcgatgcc
     1321 ggctgcgacg tgggtgaaga agccgccgta cccgcgcggt gtggggcgcg taggcgcggt
     1381 ggatgcggga ggaggtgttc ctgcgggctt ggacgctttt gccgccgacg taggctggct
     1441 ggatgagggg cctggcgctg aggccgatga gcttgtggtt ccgctggcgc tggccgcggc
     1501 agaggctccg gtcgcactcg cagcggcatt gtccttggca ggggcatcgc ccaccccggc
     1561 tttgtccttc ggggcggacg gaggcgttac ctcggtcgcc ttcaggtcaa gggtcgcatg
     1621 cgggcggctg cccgactgag cgggcgcgga acccgccgct ttcttatcgg actcgttctt
     1681 gttcgcagtc atatatgcaa gtacctcgct gcggactgat ctcgctccag gccccacacg
     1741 gggaccaagc ttttagcacg cgggtttgac gctaacctac actcctgacc atagcgttgc
     1801 tactcgggta acggcggcca gcatggcggc agaatttggg ctctcagcga cctcaactcg
     1861 cgccgggacg agtggttcga cggtggcggc aactgccggc gaaaggcaca gcaacacgat
     1921 cgtttttgcc gcttgttcga gaccttgagc ctggaccagt agggtaaaga ccgacccggt
     1981 tcgcggcgac ataaagatgg ccgcatcgat agcgccggta accatcagct ctttggttcg
     2041 ccgctggaag gcgtgggcgg ggacggcccg ataggagacg atttctcgca actcgattgc
     2101 atgagccacg agagcggccc gcaggtcgaa ggcggcgtcc tcgccgcgga cgtgcacaaa
     2161 tgcaccctgc tgcccgcgtc ccgcctcgac aatgaggggc acaagttcgc tcgcggttcc
     2221 cctaccggcg acgacttgag caaatcctag ctcgcgggcg agctgcgccg tgccggggcc
     2281 gacggccatg atgggcaacc tcagcgcggc attgaaagcc gagctggaag cgagcgcgcg
     2341 tagcccattg cggctcgtga cgatcactcc cgcgacgccg tcgagcgccg tggcgtcgat
     2401 cggcaggcgc tcgatcctca acagcggctc gagcgtcacc gcatgaccga gcgcttcgag
     2461 ctgcgcggcg catgtggagg cgtctggctc aggcctggtg agcagaacgt gcatcaggcg
     2521 gtcgcgagga aaaattctgg gccggcatgc cgcagcaact cctcggcggt ttgctcgccg
     2581 agccggattg cctcctgggc gcttccgcgg cgcgaggccg aatgtgcctg cagaccattg
     2641 ggcgtgagga tctcgccgcg gatgtgcaag gagccgccgc ttagctcggc gagacccgcg
     2701 atcggggtcc tgcaagagcc ctctaaatgt gccagatagg ctcgctcggc ggtgacacac
     2761 aatgccgttg ccgcatcgtt cagcggcgcg accagtcgca cggtcgcatc gtcgtcggca
     2821 cggatctcga tgcctatggc accctgcgcc accgcgggga gcatgacctc ggtgttcatc
     2881 gcggcggtga tgtgctgcga caggccaagc ctattgagac cggcacaagc gaggaacgtc
     2941 gcgtcggcaa cgccttcctc gagcttgcgt aagcgcgtct cgacgttgcc cctgaaaggc
     3001 acgatgcgta ggtccgggcg cagccgtagc gcttgcgcct ggcggcgcgg cgacgaggtc
     3061 ccgactacgg caccggaagg aagctcgtca aacgaagcgt acgtaatact gacgaaggcg
     3121 tcgcgcggat cttcgcgctt aaggatagcg gagatagcaa ggccctgcgg caggatcgcc
     3181 ggcatatcct tcatcgaatg tacggcaagg tcgatgtcgc cggcgaacag cgcctcctcc
     3241 agttccttgg tgaatagacc cttgccgccg gcttcgatca gcggccggtc ggtgatcttg
     3301 tcgccggtcg tccttatgat gacgagctca cattgctccg gcgagaggcc gtgcgccgca
     3361 cccaaacggg tcgccacctg ccgcgcctgg gccagtgcca ggggagagcc gcgggtgccg
     3421 atgcggatct tcgctgcttg caagccgttc tctccgggtg ctaggcgact ggccctggta
     3481 ctggccttga tgaggcgcca ttaatgcccg cgccggccct cgaacgcaaa cgcggtgtcg
     3541 ccaacgcaac ccatggcaaa tccagagcaa acaggcggca agccgagcct cgtgctcggg
     3601 ctggagacga gctgcgacga gacggccgcg gccgtcgtcc tgcgcgagcc ggatggctcg
     3661 ggccgcatcc tgtccaacat cgtgcgctcg cagtgggagc ggcatcgggc ttacggcggc
     3721 gtggttccgg agatcgctgc ccgcgcgcac gtcgagtgcc tcgacgttct tgtgcgcgaa
     3781 gcgatgcgcg aggctgacgc cggcttcggc gatcttgccg gcgttgccgc aaccacgggg
     3841 cccgggttga tcggcgggct catcgtcggc gccatcaccg gcaaggccat cgcgctggcg
     3901 cgtgggctgc cgttcgtcgc cgtgcaccac cttgaggcgc acgcgctcac agtgggtctc
     3961 accgaaggcg tagccccacc gtatctgctg cttctggttt ccggcgggca tacgcagatc
     4021 gtgctggtgc gcggcgttgg tgattaccag cgatgggcga cgacgatcga tgatgcgctg
     4081 ggggaagcgt ttgacaagac ggcaaagatg cttggactcc cctatccggg gggaccgaat
     4141 gtcgaagacg cggcgctcag cggcgatgcg gc
//
LOCUS       DATNAE010000221         3431 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3223294, whole genome shotgun sequence.
ACCESSION   DATNAE010000221 DATNAE010000000
VERSION     DATNAE010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3223294"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1084
                     /locus_tag="VN518_05055"
     CDS             <1..1084
                     /locus_tag="VN518_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03975.1"
                     /translation="QEPAPHRAVLLGLLVGLAAGSKYIGALILLFAVIAILLVPTPGP
                     ERRWLRAGTVTATAIGTFLLIMLPAIRRIARWERGVNFELVHSVEGHDVPLPLRVTWG
                     LFHLRESLWPGLGTPLFALGLIGLAAPLVAARERRMPLFLIAGFTLLWYAVHEATPLK
                     PFPDFSRYMLPLVPLFIILGTSFVYELSSRFDRRGVIAAIAVLLAAIPALSMSLRINS
                     HDVDPRTIIPPIIAASGARVVYDRYSDYQRNGRRILGLRMRPTKDTADIVVTANLAYD
                     RFEYYPARKGTVQADSAAYYRQLFTRPYLEVSNGRPTLAYFNPVVRIVAMDGPVERLE
                     KIAADIHTAAPGLTVRLIDQAPPGNE"
     gene            1104..1385
                     /locus_tag="VN518_05060"
     CDS             1104..1385
                     /locus_tag="VN518_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013417998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HXE03976.1"
                     /translation="MRVEETIREKLRGAFAPAEVEVVNDSHRHAGHHSSPGTGESHFS
                     VTVVSAAFAGKSRLERHRMVNAALAEELAGRVHALAVSALTPKERAKAS"
     gene            complement(1389..2693)
                     /locus_tag="VN518_05065"
     CDS             complement(1389..2693)
                     /locus_tag="VN518_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HXE03977.1"
                     /translation="MIMGLGITLGAILGLLIASAFFSGSETALTAASRARMHRLDEAG
                     NRRARIVTRLLASRERLIGAILLVYNLANIAASALATSLLLSLFGEVGVAYATAVMTV
                     LVVLFAEVLPKTYAIVNADRMALAVAPLINFVVKVFAPITAAMQFLVRHTLRLFGANI
                     SDDVDVLSAHEEIRGAINLHHKEGGVVKLDRDMLGGVLDLRELTVSDIMVHRTTMDAI
                     DVDLPTEQVVDAALKSSHTRVPLWRGEREEIVGILHAKTLLRALREAGGDLGKLDIMS
                     LATPPWFVPDSTSLKDQLNAFLKRKAHFAIVVDEYGEVMGLVTLEDIIEEIVGDITDE
                     TDIASIAARVQPDGSLIVDGTVPIRDVNRMMDWNIPDEEATTIAGLVIHEAQAIPYQG
                     QRFNFHGFLFEVLRRQKNRITSLQVTPLTAARKARRPQSKAS"
     gene            complement(2690..>3431)
                     /locus_tag="VN518_05070"
     CDS             complement(2690..>3431)
                     /locus_tag="VN518_05070"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HXE03978.1"
                     /translation="VVQMPTTLLAQVDSAIGGKTGIDTKQGKNLIGSFHQPSLVLADT
                     RVLFTLTPREFRAGYAEVVKYGLIGDAAFFAWLERNWPEMFSGQGGKRRHAVETSVRA
                     KARIVEQDEREQGSTRALLNLGHTFGHALEGYAGYSDRLLHGEAIAIGMRLAFTFSAE
                     QGLCSADDVARVERHLAEVGLPGRIANIPGIPPAPEQLLKLMAQDKKVKGGKLALVLV
                     RGIGQAYVERDVPMEALSAFLARECNRR"
BASE COUNT          586 a         1106 c         1107 g          632 t
ORIGIN      
        1 gcaggagccg gcgccgcatc gcgccgtcct gctcggtctc ctcgtcggac ttgccgccgg
       61 ctcgaaatat atcggcgcgc tgatcctgct cttcgccgtc attgccatcc tgctcgtgcc
      121 aacgcccgga cccgagcggc ggtggctgag ggccggcacc gtcaccgcga ccgcgatcgg
      181 cacctttctg ctcatcatgc tgccggcgat caggcggatc gcccgctggg agcggggcgt
      241 gaatttcgag ctcgtgcatt cggtcgaagg ccatgacgtg ccgttgccgc tccgcgtcac
      301 ctggggccta ttccatctcc gcgaaagcct gtggccaggt ctcggcacgc cgctctttgc
      361 cctcggcctg atcggacttg cggcgccgct cgtcgcggcg cgggagcggc ggatgccgct
      421 tttcctgatc gcgggcttca ccctgctatg gtacgccgtc cacgaggcca cgccgctcaa
      481 gccgtttccc gatttttccc gctacatgct gccgctcgtg ccgctcttca tcattctcgg
      541 cacctcgttc gtctacgagc tctcgtcgcg cttcgatcgc cgcggggtca tcgcggcaat
      601 cgccgtcctg ctcgccgcca ttccggcgct atcgatgtcg ttgcggatca actctcacga
      661 cgtcgatccg cgcacgatca ttcctcccat catcgcggcg agcggcgccc gggtggtata
      721 cgatcgctat tccgactacc agcggaacgg ccgcagaatc cttgggctac gcatgcgccc
      781 aaccaaggac acggccgaca tcgtggtcac ggcgaatctg gcttacgatc gcttcgagta
      841 ctatccggcg cggaaaggca ccgttcaggc agacagtgcg gcctattatc gacagctttt
      901 cacgcggcct tatcttgaag tgtcgaatgg acggccgacc ttggcctact tcaatccggt
      961 ggtgcggatt gttgccatgg atggaccggt ggagaggcta gagaagatcg ccgcggatat
     1021 tcatactgca gccccgggct tgacggtgcg gctgatcgat caggcgccgc ccggcaatga
     1081 atgaccgaga ggatcaggtt ccgatgcgtg tcgaagagac gatcagggaa aagttgcgtg
     1141 gcgccttcgc tccggcggag gtcgaggtgg tgaacgattc tcaccgccac gccggccatc
     1201 actcctctcc cggaacagga gagagccatt tcagcgtcac cgtcgtctcg gcggcctttg
     1261 ccggcaaatc gcggcttgag cgtcaccgca tggtcaatgc ggcgctggcc gaggagctcg
     1321 ccggccgcgt gcacgcgctg gcagtctccg cgctcacgcc gaaagagcgg gccaaagcct
     1381 cgtaaggttc aagaggcttt gctctgcggg cgcctcgctt tgcgcgccgc ggtgagcggc
     1441 gtcacctgaa gcgaggtaat gcggttcttt tgccggcgca gcacctcgaa caggaagccg
     1501 tgaaaattga agcgctgccc ctgatagggg atggcctgtg cctcgtgaat gacgaggccg
     1561 gcgatggtgg tggcctcttc gtcgggaatg ttccaatcca tcatgcggtt cacgtcgcgg
     1621 atcggcaccg tgccgtccac gatcagagac ccgtcgggct gcactcgcgc ggcgatcgag
     1681 gcgatgtcgg tctcgtcggt gatgtcgccg acgatctcct cgataatgtc ctcgagcgtg
     1741 accaggccca tgacctcgcc gtactcgtcg accacgatgg cgaaatgcgc cttgcgcttg
     1801 aggaaggcgt tgagctggtc cttgagcgag gtcgagtcgg gcacgaacca gggcggcgtc
     1861 gccaacgaca taatgtcgag cttgccgaga tcgccgccag cctcgcgcag cgccctgagc
     1921 aaggtcttgg cgtgaaggat gccgacgatc tcctcgcgtt cgccgcgcca cagcggcacc
     1981 ctggtatgcg agctcttcag cgctgcatcc acgacctgct cggtgggcag atcgacgtcg
     2041 atcgcgtcca tcgtggtccg gtgcaccatg atgtccgaga cggtaagctc gcgaagatcg
     2101 agcacgccgc cgagcatgtc gcggtcgagc ttcaccacgc cgccttcctt gtggtgcagg
     2161 ttgatggcgc ctctgatttc ttcgtgcgcc gagagcacgt cgacatcgtc gctgatattg
     2221 gcgccgaaca gccgcaaagt gtggcgcacc agaaactgca tggcggcagt tattggcgcg
     2281 aacaccttga ccacgaaatt gatgagtggc gcgaccgcaa gcgccatgcg gtcggcgttg
     2341 acgatggcgt aagtcttggg cagcacctcg gcgaacaaca ccaccagcac tgtcatcacg
     2401 gccgtggcgt aggcaacgcc gacctcgccg aacaacgaca acaacaggct ggtggccagt
     2461 gccgaagcgg cgatgttggc gaggttgtac acgagcagaa tggcgccgat cagcctttcc
     2521 cgagatgcca gcagacgggt gacgatgcgc gcacgcctgt tgccggcttc gtcgagccgg
     2581 tgcatgcgag cgcgcgacgc agcggtcagc gccgtctccg aaccggagaa gaaggccgag
     2641 gcgatcagca aaccgagaat tgcgccaagc gtgatgccca agcccatgat catcggcggt
     2701 tgcactcccg cgcaaggaac gcgctcaaag cctccatggg cacgtcgcgc tccacatagg
     2761 cctgtccaat gccgcgtacc agaacgagcg cgagcttgcc gcccttcacc ttcttgtcct
     2821 gggccatgag cttgagcagc tgttcaggcg ccggcgggat gccggggata ttggcgatgc
     2881 ggccgggtag acccacctcg gcgagatgcc gctcgacgcg ggccacgtcg tcggcagagc
     2941 agaggccctg ctcggcggag aaggtgaagg caagccgcat gccgatggcg atcgcctctc
     3001 cgtgcaggag ccggtcggag tagccggcat atccctcgag cgcatggccg aaggtatggc
     3061 cgagattgag gagcgcccgc gtgctgccct gctcgcgctc gtcttgctcg acgatgcgcg
     3121 ctttggcgcg gacgctggtc tcgaccgcgt ggcggcgctt gccgccctgg cctgagaaca
     3181 tttcgggcca gttccgctca agccaggcga agaacgcggc atcgccgatg aggccgtatt
     3241 tcaccacctc ggcgtagccc gcgcggaact cgcggggcgt gagagtgaaa aggaccctgg
     3301 tgtcggcgag aacgaggcta ggctgatgga agctgccaat caagttcttg ccctgcttcg
     3361 tgtcgatgcc agtcttgccg ccgatcgccg agtcgacctg cgcgagcagc gtagtcggca
     3421 tctgcactac g
//
LOCUS       DATNAE010000222         6186 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2027814, whole genome shotgun sequence.
ACCESSION   DATNAE010000222 DATNAE010000000
VERSION     DATNAE010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6186
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2027814"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /gene="yidC"
                     /locus_tag="VN518_05075"
     CDS             complement(<1..700)
                     /gene="yidC"
                     /locus_tag="VN518_05075"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HXE03979.1"
                     /translation="MDENNRNFILAIVLSICVLFAWQYFFVPKHPPQQQPGQQTAEQQ
                     QQPQKPAEPSPPQPRAEGETPSPAGTAVAPGGAQAVTETREEALASSQRVAIDSPGIK
                     GSINLKGARIDDVVLKKYHETVDPSSPNVVLLSPAGSPHPYYVEHGWVAETGKDTTVP
                     GPDTVWKAQSIGPLTVSTPVTLSYDNGKGLVFTRTIAIDDNFMFTVTDAVANSGSEPV
                     TLYPYGLVSRLGVPI"
     gene            complement(782..1141)
                     /gene="rnpA"
                     /locus_tag="VN518_05080"
     CDS             complement(782..1141)
                     /gene="rnpA"
                     /locus_tag="VN518_05080"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HXE03980.1"
                     /translation="MTAIERLKTRAEFLHVRDGSRFAAPSLVLQARARGEKGGALPDV
                     ARFGFTASKALGGAVLRNRARRRLKEAVRIVGPEHAVPGYDYVLIARAGTVQRRFTEI
                     TRDLERALTKVHQAPRP"
     gene            complement(1174..1308)
                     /gene="rpmH"
                     /locus_tag="VN518_05085"
     CDS             complement(1174..1308)
                     /gene="rpmH"
                     /locus_tag="VN518_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010659431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HXE03981.1"
                     /translation="MKRTYQPSRLVRKRRHGFRARMAAKGGRKVLARRRAKGRKRLSA
                     "
     gene            complement(1438..1695)
                     /locus_tag="VN518_05090"
     CDS             complement(1438..1695)
                     /locus_tag="VN518_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03982.1"
                     /translation="MVVRFYAVACIALASLLLGGTGMARAGDDYSSPQAGDSYSPPDA
                     GEQPAQPDAAPPPEDDSATAPDDNSGGDSGDEAPPPSDQPE"
     gene            1888..2679
                     /locus_tag="VN518_05095"
     CDS             1888..2679
                     /locus_tag="VN518_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007010584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HXE03983.1"
                     /translation="MAAASNKENTALAWLKRLWPLFLLLAGMAFVFAMGWHRYLTLQE
                     LVTRREALSAAIAGHTILAVLAFMAIYTATVALSLPGAAVLTLAGGFLFGWFWGGLAS
                     MLAASAGAVLVFLIARTALGEPLAARAGPWLAKLRQGFQEDAFNYLLFLRLVPIFPFW
                     LVNLAPALLGVSLWTYTLATVIGIVPGSFAYSIAGKGLDSVIVAQQAAHQSCLAKMGS
                     DAEELCPFVLEPRALLTPGLVAGFVALGLVALIPIAVKRFRRKPA"
     gene            2709..4133
                     /locus_tag="VN518_05100"
     CDS             2709..4133
                     /locus_tag="VN518_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXE03984.1"
                     /translation="MEEVLQPDLCVIGAGSGGLSVAAAAAQFGVPVVLIEKGRMGGDC
                     LNYGCVPSKALLAAGKRAEAVRTAGAFGIDVIEAQVDHARVHDYVHGVIAAIAPNDSV
                     ERYTGLGVRVLQQQASFLDKNTVEAGHYLIRARRFIVASGSSPVIPPIPGLDEVPYLT
                     NETIFDVSERIQHLIVLGGGPIGMELAQAHRMLGAHVTIVDAATILGHEDPEASRIVI
                     DRFTEQGVLLHPGTRVERVERAIQGVAVHIGKQGETEVIKGSHLLVATGRKPNVKGLS
                     LERAGIVYDARGIKVNQQLKTTNHRVYAIGDVTGGAQFTHLANYHAGIVIRNALFRLR
                     PRASATAIPRVTYTDPELAQIGLTEAEARTKYRRIRILRWPYADNDRAQAEATTHGFI
                     KVITRPNGQILGCTIVGAEAGELLQLWVMALARDMRIGDLTGLVLPYPTLSELSKRVA
                     YTFYQPSLTKGWLRRVIDFLRRFG"
     gene            4188..5651
                     /locus_tag="VN518_05105"
     CDS             4188..5651
                     /locus_tag="VN518_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HXE03985.1"
                     /translation="MSDDVRSAGKDKNEPIRYGPGLSGKLLLLTIVFVMVAEVLVFVP
                     SVSNFRRQWLMARLEDAQIAALAAEAAPGGQLPKTLRDELLQSVKVKAVAVKRADSRR
                     LILEEDMPAEIDASYDLRDATWLALIADALMVYAEPADRVIRVVGKPNFATGEFIEVV
                     MEEAPLKAAMIGYGLDILGLSILISIITAALVYLTLDMLLVKPMTKLTWNMVRFSERP
                     EDPTRIVTPSGRRDEIGTAERELAAMQAEIAGMLSQKNRLAALGLAVSKISHDLRNML
                     SSAQLLSDRLRSVKDERVQQLVPKLLASLDRAIRLCARTLDFGQAHETPPKRKHFELA
                     PLVAEVGEGLGLPQPERIEWISDIPAELKVDADRDQLFRVLSNLCRNAVQALESDADG
                     MGEIVITARREGSVTLIDVRDTGPGVSERARAHLFQAFQSVARKGGTGLGLAIAAELI
                     NAHGGQIMLVSNEGGATFRVIIPDVVVELQRARRRSA"
     gene            5800..5876
                     /locus_tag="VN518_05110"
     tRNA            5800..5876
                     /locus_tag="VN518_05110"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5834..5836,aa:Arg,seq:acg)
BASE COUNT         1021 a         2019 c         1990 g         1156 t
ORIGIN      
        1 tgatcggcac gccgagcctg gagacgaggc cgtaggggta gagcgtcacc ggctccgagc
       61 cgctattggc caccgcgtcg gtcaccgtga acatgaaatt gtcgtcgatc gcgatggtgc
      121 gggtgaagac gagccccttg ccgttgtcat agctgagcgt gaccggggtc gagacggtga
      181 gcgggccgat cgactgagcc ttccacaccg tgtctgggcc aggcacggtc gtgtccttgc
      241 cggtctcggc cacccagcca tgctccacat aatagggatg cggcgagccg gccggagaca
      301 gcagcaccac gttcgggctg ctcgggtcga cggtctcgtg gtacttcttg agcaccacgt
      361 cgtcgatgcg cgcgcccttc agattgatcg agcccttgat gcccggggag tcgatggcga
      421 cgcgttggct tgaggcaagc gcctcctcgc gcgtttcggt aacggcttgg gcgccaccgg
      481 gcgcaactgc cgtgccggcc ggggaagggg tttcgccttc ggcgcgcggc tgcggcgggc
      541 ttggctccgc cggcttctgc ggctgttgct gttgctctgc cgtctgttgc cccggctgct
      601 gctgcggcgg atgcttgggc acgaagaaat attgccacgc gaacagcacg cagatggaca
      661 agacgatggc gaggatgaaa ttgcggttgt tttcgtccat gtcgctttcg ctgttggccg
      721 cgggctgcgc ggtcagagtg gcaacggcct aagggtcctg tcggaaggct ttcagcgctg
      781 tctagggcct cggcgcctga tggaccttgg tcagcgcccg ttcaaggtcc ctggtaatct
      841 cggtgaagcg ccgttgtacc gtgcccgccc gagcgatcaa gacataatcg tagccgggaa
      901 cggcgtgctc cggaccaaca atccgcaccg cctccttcag ccgccgccgc gcccggttgc
      961 gcaggactgc cccgcctaag gccttgctcg cggtgaagcc gaagcgggcg acgtcaggca
     1021 gcgcgccgcc cttctcgccc cgcgcccggg cctgcagcac gagcgaaggc gcggcaaaac
     1081 gcgatccgtc cctcacatgc agaaattcag cgcgggtctt gaggcgttcg attgccgtca
     1141 ccgtggaggc ggggctcccg cgctagcggc cgatcaagcc gacaggcgct tgcgtccctt
     1201 ggccctgcgc cgtgccagca ccttgcggcc gcctttggcc gccatgcggg cgcgaaagcc
     1261 gtggcgccgc ttgcggacga gccgcgatgg ttgataggtg cgtttcacgc caaagctccg
     1321 atggatgcag ggtttggcgg cttataggtg gcaggaacgg ccaagtcaat ccgcgggcat
     1381 ggcggttgtg aggctgcatc cctccccaag cagaggaggg atgcggcagg cgtgaaatca
     1441 ttcaggctgg tctgacgggg gcggagcctc gtcgccgcta tcgccgccgc tattgtcatc
     1501 aggcgcggta gcgctgtcat cttccggcgg aggggcggcg tcgggctggg ccggttgttc
     1561 gccggcatcg ggcggcgaat aggagtcgcc ggcttgagga ctggagtaat catcgccggc
     1621 tcgagccatg ccggttcctc ccagaagcag agaagcgagt gcgatgcagg caacggcata
     1681 aaatcgcacg accatgctat cctccttgtg tctcaggctt aagcgcctga ttgcggcatc
     1741 atacaaggct cacggaatcg ccaaactgcg acaagagcgc cttgccccat cacgagtggt
     1801 tacggggcat caattcggcg cgataggcgg tttcgcgggc tgcctcctgt cgcttattga
     1861 gggacgttat tggcgttgag gagcgacatg gcggccgcga gcaataagga gaacaccgcg
     1921 ctcgcctggt tgaagcggct ttggccgctc ttcttgctgc ttgccggcat ggccttcgtt
     1981 ttcgccatgg gctggcaccg ctacctcacg ttgcaagagc tggtgacgcg ccgcgaagcc
     2041 ttgtctgcgg cgatcgccgg ccacaccatc ctcgccgtcc tcgccttcat ggcgatctat
     2101 accgcgaccg tcgccttgtc cctgccggga gccgcggtcc tcacgcttgc cggagggttt
     2161 ctgttcggct ggttctgggg tggactcgcc tccatgctcg ccgcctcggc gggcgccgtc
     2221 ctcgtgttcc tcatcgcgcg caccgccctt ggcgagccgc tcgccgcccg ggccggccct
     2281 tggcttgcga aactgcgcca gggcttccag gaggatgcgt tcaactacct cctgttcctg
     2341 cgcctcgttc ccatcttccc gttttggctc gtcaatctcg ccccggcgct tcttggcgta
     2401 agcctttgga cctacacgct tgccacggtc atcggcatcg tcccgggaag ttttgcctat
     2461 tccatcgccg gcaaaggcct tgacagtgtt atcgtggctc agcaagcggc gcaccaatcc
     2521 tgccttgcca agatgggatc agatgcggaa gagttatgcc cgttcgtgct tgagccccgg
     2581 gccttgctca cccctgggct cgttgcgggc tttgtggcgc ttgggctcgt tgcgctgatc
     2641 cccattgcgg tcaagcgttt caggcgcaag ccggcgtagg gcgtgagcca cggggaagag
     2701 gggctaacat ggaagaggtg ctgcaaccgg acctctgcgt gatcggcgca gggtcgggag
     2761 gcctctctgt ggctgccgcg gcggctcaat tcggcgtccc tgtcgtgctc atcgagaagg
     2821 gccgcatggg cggcgactgc ctcaattacg gctgcgtgcc gtcgaaggcg ctgctcgccg
     2881 ccggcaagcg tgccgaggcc gtgcgcaccg ctggagcctt cggcatcgac gtcatcgagg
     2941 cgcaggtcga ccacgcccgg gtgcatgact acgtgcatgg cgtgatcgcc gccatcgcac
     3001 cgaacgattc cgtcgagcgc tacaccgggc tcggcgtccg cgtcctccag cagcaggcaa
     3061 gcttccttga caagaacacc gtggaggccg ggcattacct gatccgcgcc cgccgcttca
     3121 tagtggcaag cggttcctcg cccgtcatcc cgccgatccc aggtctcgac gaggtgcctt
     3181 atctcaccaa cgagacgatc ttcgacgtga gcgagaggat ccagcatttg atcgtgctcg
     3241 gcggtggccc catcggcatg gagctcgccc aggcccatcg catgctcggc gcccacgtca
     3301 ccatcgtcga tgccgccacc atcctcggcc acgaggaccc tgaagcgagc cgcatcgtca
     3361 tcgatcgctt caccgagcag ggcgtgctgt tgcatcccgg cacgcgggtg gagcgcgtcg
     3421 agcgcgccat acagggcgtc gccgtgcata tcggcaagca gggcgagacc gaggtgatca
     3481 agggaagcca tctgctggtc gccaccggac gcaagcccaa cgtcaaaggc ttgagcctgg
     3541 agcgggccgg catcgtctac gacgcccgcg gcatcaaggt gaaccagcag ctcaagacca
     3601 ccaaccaccg cgtctacgcc atcggcgacg tgaccggcgg cgcgcaattc acccatctcg
     3661 ccaactatca cgccggcatt gtgatcagga atgcgctgtt ccgcttgcgg ccgcgcgcga
     3721 gcgccaccgc catcccgcgc gtcacctata ccgatcccga gctcgcccag atcggcctca
     3781 ccgaagccga ggcgcgcacc aaatatcgcc gcatccgtat cctgcgctgg ccctatgccg
     3841 acaacgaccg cgcccaggca gaggccacca cccacggctt catcaaggtc atcaccaggc
     3901 cgaacgggca gattctcggc tgcaccattg tcggcgccga ggccggcgag ctgctccagc
     3961 tttgggtgat ggcgctcgca agagacatga ggatcggcga cttgacgggc cttgtgctgc
     4021 cttatcccac gctgtcggag ctgtcgaagc gcgtggccta taccttctat caaccttcgc
     4081 tgaccaaagg ctggcttcgc cgcgtcatcg atttcttgcg ccgcttcgga tagcattggg
     4141 cgaggcgcgg gtttgcgccc ttgagcttgg cggagcgagt gcatcacgtg agcgacgacg
     4201 ttcggagtgc cggcaaggat aagaacgagc ccatccgcta tgggcccggg ctttccggca
     4261 agctgctgct gctgaccatc gttttcgtca tggtggccga ggtgctcgtc ttcgtgccct
     4321 cggtgtcgaa cttccgccgc caatggctga tggcccggct cgaggatgcg cagatcgcgg
     4381 cccttgccgc cgaagcagcg cccggcggcc agctgcccaa gaccctgcgc gatgagcttc
     4441 tgcaaagcgt caaggtgaag gcggtggccg tgaagcgggc cgactcgcgc cggctgatcc
     4501 tcgaggagga catgccggcc gagatcgatg cctcctacga tctccgcgac gccacctggc
     4561 tcgccttgat tgccgacgcg ctcatggtct acgccgagcc tgccgaccgg gtgatccgcg
     4621 tcgtcggcaa gccgaacttc gccaccggag agttcatcga ggtggtgatg gaggaggctc
     4681 cgctcaaagc ggcgatgatc ggctacggcc tcgatattct cgggctctcg atcctgatct
     4741 cgatcatcac cgcggcgctc gtctacctca cgctcgacat gcttctggtc aagcccatga
     4801 ccaagctcac ctggaacatg gtgcgtttca gcgaacggcc ggaggaccca acacgcatcg
     4861 tcacgccttc tgggcggcgc gacgagatcg gcaccgccga gcgcgagctt gccgccatgc
     4921 aggccgagat cgccggcatg ctgagccaga agaaccggct cgcagccctt ggcctcgccg
     4981 tgagcaagat cagccacgat ctgcgcaaca tgctttcaag cgcgcagctc ctctccgacc
     5041 gcttgcgcag cgtcaaggat gagcgcgtgc agcagctcgt gccgaagctg ctcgcttcgc
     5101 tcgatcgtgc catcaggctt tgcgcccgta ccctcgattt cggccaggca catgaaaccc
     5161 cgcccaagcg caagcatttc gagcttgcgc cgctcgtggc cgaagtcggc gaggggcttg
     5221 gcttgcccca gcccgagcgc atcgagtgga tctcggacat tccggccgag ctcaaggtcg
     5281 acgccgaccg cgatcagctc tttcgcgtct tgagcaatct ctgccgcaac gccgtgcagg
     5341 cgctcgagag cgacgccgac ggcatgggcg agatcgtgat caccgcgcgg cgcgagggct
     5401 cggtcacttt gatcgatgtg cgcgataccg ggcccggcgt gtcggagcgg gcgagggcgc
     5461 atctcttcca ggcattccag agcgtggcgc gcaaaggcgg caccggactt gggctcgcga
     5521 tcgctgccga gctgatcaac gcccatggcg gccagatcat gctggtgagc aacgaaggcg
     5581 gcgccacctt ccgggtgatt attcccgacg tggtggtcga gcttcagcgc gcccggcgcc
     5641 gcagcgcttg agggctcgac gctttgccgt gcctcatttt tggggatttt ccggcgtcgc
     5701 gcggcttgcc ttcatctgat caaggcatta ggtagcttga ttggccgccc ccttggtgtg
     5761 ccatcccttc gtgccgcgcg acgcggcccg tggctaaaag cgcccgtagc tcagctggat
     5821 agagcaccag actacgaatc tgggggtcgg aggttcgaat ccttccgggc gcgccaataa
     5881 aatcaataag ttaatgcttt tgccgtgtag gctggtgcga gcttggttac caaatggtca
     5941 ccacctcgga caacgagtca tggccccctt tactgacgtc acagtaaaga gctgggtgat
     6001 tggcgggccc gccgcatcgg accaaaaact cttctccagc cgttgagcat cctctccgcg
     6061 aggccaccat catagtgaat ccgacgcatg atcgaatgag cgataggaac cgagttctga
     6121 gcgcgaaacg ctcatggcgc ggcactgtca cgttaagcgg aaattcgcag gccaaatgac
     6181 cgtggc
//
LOCUS       DATNAE010000223         2147 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_825671, whole genome shotgun sequence.
ACCESSION   DATNAE010000223 DATNAE010000000
VERSION     DATNAE010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_825671"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="VN518_05115"
     CDS             complement(<1..640)
                     /locus_tag="VN518_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HXE03986.1"
                     /translation="MNDSPSDKEKNRLSARALRYGKVGANVGGVAARIAGRRLFGLDP
                     ERDKDAAALAAALGGLKGPIMKVAQLLATIPEALPAEYAAELGQLQSQAPPMGWPFVR
                     RRMAAELGPGWEAKFASFEHEAAASASLGQVHRARSHDGRALAAKLQYPDMQSAVEAD
                     LAQLSILFSIHRRMSPAIETSEMLKEISARLREELDYDLEARHTRLYGLIFKE"
     gene            complement(766..1353)
                     /locus_tag="VN518_05120"
     CDS             complement(766..1353)
                     /locus_tag="VN518_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6790 family protein"
                     /protein_id="HXE03987.1"
                     /translation="MTQVSLVPILLWVAALVCAGLAIWREPRPVLPSFAVDRLLRYVF
                     LLPLGVQGVWAFIGHVFFPQQSAASIGWAPSPFQYEVGVANLGLGLACIYAAFKGFEA
                     RVAAGILAACFLFGAGIGHVRDIVTAGNLAPGNAGPIMLTDFLTPIAVFALLFFASDR
                     WKPKSAATLALEAELDIARKAMRDYRQALSQLGKD"
     gene            complement(1438..>2147)
                     /locus_tag="VN518_05125"
     CDS             complement(1438..>2147)
                     /locus_tag="VN518_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006199443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HXE03988.1"
                     /translation="AHPYILLNYQGKPRDVMTLAHELGHGVHQVLANVQGPLMAPTPL
                     TLAETASVFGEMLTFRKLLDQAPTPRERKALLASKVEDMINTVVRQIAFYSFERRVHT
                     ERREGELTSDRLGEIWLEVQRESLGPAIELKPGYETFWCYIPHFIHAPFYVYAYAFGD
                     CLVNSLYAVYEKAEKGFAERYLDMLAAGGTKHHKELLAPFGLDASDPNFWSMGLKVIE
                     GLIDELEAMDKTATAKH"
BASE COUNT          387 a          716 c          686 g          358 t
ORIGIN      
        1 cctccttgaa gatcaacccg tagagccgcg tgtggcgagc ctcgagatcg taatcgagct
       61 cctcgcgcag ccttgcagag atctccttca acatctcgga ggtttcgatc gccgggctca
      121 tgcggcgatg gatcgagaac aggatcgaaa gctgagcgag gtcggcctcg acggcggact
      181 gcatgtccgg atattgcagc ttggcggcga gcgcgcggcc atcgtgcgat cgcgcgcgat
      241 gcacctggcc gagcgaggcc gacgccgcgg cttcatgctc gaagctagcg aacttcgctt
      301 cccaaccggg tcccagctcc gccgccatgc gccggcgcac gaacggccag cccatgggcg
      361 gcgcctggct ctgcagctgg ccaagctcgg cggcatattc ggccggcagc gcttcgggaa
      421 tggtggcgag cagctgcgcc accttcatga tcgggccttt cagtccgcca agcgccgcgg
      481 cgagcgcggc cgcatccttg tcgcgctcgg ggtcgagccc gaacagacgc cgtccggcga
      541 tgcgcgccgc cacgccgccg acattggcgc cgactttgcc gtagcgcagc gcccgcgcgc
      601 tcagtcgatt cttctccttg tcggaggggc tgtcgttcat ggggaaactt cgcgggcctt
      661 cacgcgtgac taaagggtcc ccttcatata gggggcccaa tcccggtatg ccgcccgctt
      721 gcgacttttg cagccgccag caagaggcgg ccggctggcg cgatctcagt ccttgccgag
      781 ttggctcaag gcttggcgat agtcgcgcat cgccttgcgg gcgatatcga gctcggcttc
      841 gagcgcaagc gttgccgccg acttcggctt ccacctgtcg ctggcaaaga agagcagcgc
      901 gaaaacggcg atgggtgtca gaaaatcggt gagcatgatc ggcccggcat tgcccggagc
      961 gagatttcca gccgtgacaa tgtctctgac gtgaccgatg cccgcgccga acaggaagca
     1021 ggcggcgagg atgccggcgg ccacccgcgc ctcgaagccc ttgaaggcgg catagatgca
     1081 ggcgagaccg agcccgagat tggcgactcc gacttcatat tggaaagggc tcggggccca
     1141 gccgatcgac gcggcggatt gctgcggaaa gaacacgtgg ccgatgaagg cccacacgcc
     1201 ctgcacgccg agcgggagga ggaacacata gcgcagcagc cggtcgaccg cgaagctcgg
     1261 caagaccgga cgtggctcac gccaaatcgc taggcccgcg caaacgagcg ccgcgaccca
     1321 caataaaatg ggaaccagag aaacctgcgt caccgcgata aacccccctc aacccgttat
     1381 cgcgactcca ccgcgtcaat ccctccccgt caacctctcc gcagggtgag gctcgcccta
     1441 atgcttggcg gtcgccgtct tgtccatggc ttcgagctcg tcgatcaggc cctcgatcac
     1501 cttgagcccc atcgaccaga aattcgggtc ggacgcatcg agcccgaacg gcgccagcag
     1561 ctccttgtgg tgcttggtgc cgcccgccgc gagcatgtcg agatagcgct cggcgaagcc
     1621 cttctccgcc ttctcgtaga cggcataaag cgagttcacc aggcaatcgc cgaaggcgta
     1681 ggcatagacg tagaacggcg cgtggatgaa atgcgggatg tagcaccaga aggtctcgta
     1741 gccgggcttg agctcgatgg ccgggccgag gctctctcgc tgcacctcga gccagatctc
     1801 gccgagccgg tcgctggtca gctcgccctc gcgccgctcg gtgtgcaccc ggcgttcgaa
     1861 actatagaac gcgatctgcc tgaccacagt gttgatcatg tcctccacct tggacgcgag
     1921 cagcgccttt cgctcacgcg gcgtcggcgc ctggtcgagc agcttgcgga aggtcagcat
     1981 ttcgccgaac acgctcgccg tctcggccag cgtcagcggc gtcggcgcca tcagcggccc
     2041 ttgcacattg gccagcacct gatgcacgcc gtggccgagc tcatgcgcga gcgtcatcac
     2101 gtcgcgcggc ttgccctgat agttaagcag gatatagggg tgcgcgc
//
LOCUS       DATNAE010000224         3667 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3229462, whole genome shotgun sequence.
ACCESSION   DATNAE010000224 DATNAE010000000
VERSION     DATNAE010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3667
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3229462"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..979)
                     /locus_tag="VN518_05130"
     CDS             complement(<1..979)
                     /locus_tag="VN518_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009208986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXE03989.1"
                     /translation="MRPALLAFLIATIAAFGFGEPVQAATKVTVGVLKFGTVSWVLDT
                     IQKNGLDKAEGFELDVVPLASSEATAVGLQGGSVDIIATDWLWVSRERSGGADFTFSP
                     FTTALGAIMVPPNSPIKTLTDLKGKKLGVAGGPLDKSWLLIVAYALRTANLDLRTDTT
                     QVFGAPPLLAEKAKQGEIDAVLNFWPYAARLEAAGFKQLIGIEDVVSELGAKGEVAMV
                     GYVFSESWANENLKAIDGFLRAAAKADELLATSNEAWEAVSPLMRAEDQATFEALRRR
                     YREGIPERSTAENESDAKVLYQFLRELGGEKLVGPGTELAPGTFWHDGKP"
     gene            complement(1237..1917)
                     /locus_tag="VN518_05135"
     CDS             complement(1237..1917)
                     /locus_tag="VN518_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008835078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXE03990.1"
                     /translation="MNVLLVEDEPRVADFIDRGLRAENHGVTVAATGSEGVDLAKTGE
                     FDVIVLDLMLPDMHGYDVCQRLRLEGDHTPILMLTAMDTLEDKIKGLRVGADDYLAKP
                     FDFDELLARIEALVRRSRNFAPRSNVLQVADLVLDRDLLEVKRGGEEIKLTAKELAIL
                     ELLMGAPGRVFSRTRILNQVWGYSEDPLTNVVDVYIARLRRKIDTPGREPLLETVRGH
                     GYRLRAAA"
     gene            complement(1914..3512)
                     /locus_tag="VN518_05140"
     CDS             complement(1914..3512)
                     /locus_tag="VN518_05140"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HXE03991.1"
                     /translation="MMRYQTKLLITACLLLALSLSAAGLAYWGVSQSHSYLSRSRIAH
                     QELERQLQLSRHSQQLFKAWTDTLLTGISDRPLGAAYLHQVIESDLAELDRLTQTELA
                     MVGSEERRMETAELDRLNAIKSEFQHALDQLGEVEQLRRRGRPDVAWSTLLALLKGGI
                     DKKLNELIEVAVADETAEVVRIDADAARLLSRLEQISQIHAVLAILITLALVMLLLQR
                     LRAPLAELLRGTRVLAEGDLSHRIAISGRDEFADLGQSFNRMADDLQSHQKALQSAHA
                     NLEAQVAERTEELHQANQSLRKVDETRRAFFADISHELRTPLTVIRGEGEIALRGRNK
                     PVAEYKNSISRIVQQAKHLSVLVNDLLFIARHGAGAARLNLQTIDLGNLLEKVCGDAK
                     VIAHPKAVDITYANGSAEELVHGDPARLRQLFMVLLDNAVRYSNSKGEVKVDIASAGG
                     EVRVRVSDRGIGIAPEELEGIFERFRRGGNASEHNEEGLGLGLPVAKAIVEAHKGRIE
                     MNSRLGEGTTVTVALPASPAEGVA"
BASE COUNT          650 a         1158 c         1173 g          686 t
ORIGIN      
        1 atggcttgcc gtcgtgccag aacgtgccag gcgcaagctc ggtcccgggg ccgacgagct
       61 tctcgccacc aagctcgcgc aggaattgat agaggacctt ggcgtcggat tcgttctcgg
      121 ccgtcgagcg ctcggggatg ccctcgcggt agcgccgccg gagcgcctca aacgttgcct
      181 ggtcttccgc ccgcatcagg gggctgaccg cctcccacgc ctcattcgac gtcgcgagca
      241 gctcgtcggc cttggccgcc gcgcgcagga agccgtcaat ggccttcagg ttctcgttag
      301 cccagctctc gctgaacaca tagccgacca tggccacctc gcccttggcg ccgagctcgc
      361 tcaccacgtc ctcgatgccg atcagctgct tgaagcctgc cgcctcgagc cttgcggcat
      421 aaggccagaa attgagcacg gcgtcgattt cgccttgctt cgctttctcg gcaagcaggg
      481 gcggcgcgcc gaacacctgc gtcgtgtccg tcctgagatc gagattggcg gtgcgtagcg
      541 cataggccac gatcaaaagc cagctcttgt cgagtgggcc gccggcgact ccgagcttct
      601 tacccttgag gtcggtcaga gttttgatcg gggaattggg gggaaccatg atggcgccga
      661 gcgccgtggt gaagggcgag aaggtgaagt cggcgccgcc gctccgctcg cgcgacaccc
      721 acagccaatc cgtggcgatg atgtcgaccg agccgccttg taggccgacg gcggtcgcct
      781 cggagctcgc gagcggtacg acgtcgagct cgaagccctc ggccttgtcc aggccgttct
      841 tctggatagt gtcgagcacc cagcttacgg tcccgaattt gaggacgccg accgtgacct
      901 tggtcgccgc ctggaccggc tcgccgaaac cgaacgctgc aatggtggca atgaggaatg
      961 ctaacagagc tggacgcaac gggcgctcct tccgcgttgt ttcttcttca gtcccaaatc
     1021 gccgggattc gccacgagat gagcgggcag ctccaatgcc acccaaagcg gcagacgctg
     1081 cttgtaactc gcgcaggcga ggcccggaat gggtatggcg cgtgaccgcg agagtggcgg
     1141 ggcatactct acccatccag ccgaactaag caagatttgc ctggcaagat tgccaaactt
     1201 ccgggtgtgt tttgccaaac cctgcttgcc tcgcgtttat gctgcagccc tgaggcgata
     1261 gccatggccg cgcaccgttt ccagcagcgg ctcgcggccc ggggtgtcga tcttgcggcg
     1321 gagcctggcg atatagacgt ccacgacgtt ggtgagcggg tcttcgctgt agccccagac
     1381 ctgattgagg atgcgcgtgc gcgagaacac gcggcccggc gcgcccatca acagctcgag
     1441 gatggcgagc tccttggcgg tgagcttgat ctcttcgccg ccccgcttga cctcaagcag
     1501 atcgcgatcg agcacgagat cggcaacctg cagaacattc gatcgcggag cgaaattgcg
     1561 cgagcggcgg accagcgcct cgatgcgggc gagcaactca tcgaaatcga agggcttggc
     1621 gagataatcg tcggcgccga cgcgcaggcc tttgatcttg tcctcgagcg tgtccatggc
     1681 ggtcagcatc agaatgggtg tgtgatcgcc ctccaggcgc aagcgctgac agacgtcata
     1741 gccgtgcatg tcgggcaaca tcaaatccag cacgatcacg tcgaactcgc cggtcttggc
     1801 gagatcgacc ccctcggatc cggtagcggc aacggtgacg ccgtgatttt cggcgcggag
     1861 cccgcgatcg atgaagtcgg cgacccgcgg ctcgtcttcg accagaagga cgttcacgcg
     1921 acaccctccg ccggagaggc cggcagtgcg accgtgacgg tggtcccttc gcccaaacgg
     1981 ctgttcattt cgatccttcc cttgtgggcc tcgacgatgg ccttggctac cggcagaccc
     2041 aagcccaagc cctcttcgtt gtgctcgctg gcgttgccgc cgcggcgaaa ccgctcaaaa
     2101 attccttcca gctcttccgg cgcgatgccg ataccgcgat cggagacgcg cacccgcacc
     2161 tcgccgccag cgctcgctat atcgactttc acctcgcctt tggaattcga atagcggaca
     2221 gcgttatcca agagaaccat gaaaagttgc cggagccttg ccggatcgcc atgcacaagc
     2281 tcctcggccg atccattggc gtaggtaatg tcgaccgcct tcggatgggc gatgaccttg
     2341 gcgtcgccgc acaccttctc gagcaaattg ccaagatcga tggtctgcag gttaaggcgc
     2401 gcggcgccgg ccccgtggcg ggcgatgaac agaaggtcgt tcaccagcac ggatagatgc
     2461 tttgcctgct ggacgatgcg cgagatcgag ttcttatact cggcgaccgg cttgtttcgc
     2521 ccacgcagcg cgatctcgcc ttcgccgcga atgacggtga gcggcgtgcg cagctcgtgg
     2581 ctgatatcgg cgaagaaagc gcgccttgtc tcgtcgacct tgcgcaatga ttggttcgcc
     2641 tgatgcagct cctcggtgcg ttccgccacc tgggcctcga gattggcgtg ggcgctttgc
     2701 agcgccttct ggtgcgattg gaggtcgtcg gccatgcgat tgaagctttg gccgagatcg
     2761 gcgaactcat cgcgcccgga aatggcgatg cgatgagaga gatcgccctc ggcaagcacg
     2821 cgcgtgccgc gcaacagctc ggcgagcggg gcgcggagcc gctgcaggag cagcatcacc
     2881 agcgcgagcg tgatcagaat ggcgaggacc gcgtgaatct gactgatctg ctcaagccgg
     2941 ctcagcagcc gcgcggcatc ggcatcgatc ctgacgacct cggcggtctc gtcggcgacc
     3001 gcgacctcga tcagctcgtt caacttcttg tcgatgccgc ccttgagcag agcgagcagc
     3061 gtcgaccagg cgacatcggg gcggccgcgg cggcgcaatt gctccacctc gccgagctgg
     3121 tcgagtgcgt gctgaaattc gctcttgatg gcgttgagcc ggtcgagctc ggcagtttcc
     3181 atcctccgtt cctcggagcc gaccatggcg agctcggttt gggtgagccg atcgagctcg
     3241 gcgagatcgg actcgatgac ctggtgcagg taggcggctc caagggggcg gtcgctgatg
     3301 ccggtgagaa gcgtgtcggt ccaggccttg aacagctgct ggctatgccg ggaaagctgc
     3361 aactggcgct ccaactcctg gtgggcgatg cgcgagcggc taagatagct gtgcgactgg
     3421 ctcacgcccc aataggcaag tcccgccgcc gagagcgaca aggcgagcag caggcacgcc
     3481 gtgatcagga gcttggtctg gtagcgcatc ataaaactca gagtttctgt ggcggatgag
     3541 caacagaaac tcgctgatct tcaccttggg gcaagcccac gctttacggg caccggcccc
     3601 gtgtcagctc ccacgttata ctgggttttg actggtaact aagggcgtct ttcagcccgc
     3661 aggcgcg
//
LOCUS       DATNAE010000225         2352 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2331407, whole genome shotgun sequence.
ACCESSION   DATNAE010000225 DATNAE010000000
VERSION     DATNAE010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2331407"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1577
                     /locus_tag="VN518_05145"
     CDS             <1..1577
                     /locus_tag="VN518_05145"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03992.1"
                     /translation="LAIKFLVLGVLAVYARAGAVALRRPISDFYVAGRLVPGLFNGMA
                     IALSLIPVLAFAELAGALSQGWDGASLLVLGGAGGLLIIAFLLAPYLRKFGGYTIADF
                     LGERFGGSGVRPLAVLAVILCSFPALALVLSGLGVIAAQIFAVDMGTGIALGVAMLLL
                     CSFAAGMRSASVTQILQYAVLLAVAVVALGLMVWQQGAVFSGAEPAGLKAALGILKLD
                     SFAAEGSLNRLALLFCLVAGVASLPHLLMRGLVTPSIEEARTSFLWALPFAGVLLLLA
                     APFLSLFEAAPTQESPASAILSFGLVAVGAISLLLAVGSGLVLAIANALSYDLYYRSL
                     HLSASTERRILVARAAVVLTAALAAGVTFAAPDVIPLMTGASFSLAASAFLPALLLGV
                     WWKRATGEGALAGMLAGLAVCLYYLLAPRYIPFAFYETSSFLSNATPDEAAHYVALRQ
                     GYYLADASGREAALSTWRAMALQLANWGGVKREFAALFAVPLGFAVMIGVSLFTPAPS
                     RDVQSFVEDLRKPQVA"
     gene            complement(1581..2180)
                     /locus_tag="VN518_05150"
     CDS             complement(1581..2180)
                     /locus_tag="VN518_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE03993.1"
                     /translation="MSIRSATLGLALLATTVLPALAEDAPAAAPQELQWTEFQSPERG
                     FSVSFPGAPKTTTAPVEGQNPLIQYDFQVSVGDDTVYSVVVFEYPAGKGPNPDTDAFV
                     KLVNAYAKGSESRLRKRSTATIDGRSGFEAIADDNKSKLSHLIDVVPAGDRVYMLVSA
                     GPKSHATSDEAERFRDSFHVLDGGASAAPAQSAANPPAQ"
BASE COUNT          293 a          778 c          782 g          499 t
ORIGIN      
        1 cattagcgat caaatttctc gtgctgggcg tcttggcggt ttatgcccga gccggggcgg
       61 tcgcgttacg gcggccaata tccgacttct acgtcgcggg gcgactcgtg ccgggcctgt
      121 tcaacggcat ggcgatcgct ctgtcgctaa tccccgtgct cgcctttgcc gagcttgccg
      181 gtgcgctgag ccagggatgg gatggggcat cgctgctggt gctcggcggg gcgggaggcc
      241 tcctcatcat cgcgtttctt cttgcgccct atctccgcaa gttcggcggc tacacgattg
      301 ccgattttct cggcgaacgg ttcggcggct caggcgtcag gccgctcgcc gtgttggccg
      361 tgatcttgtg ctcgttcccc gcgctcgctt tggtgctgtc cggcctgggc gtcatcgccg
      421 cgcagatctt tgccgtcgac atgggcaccg gcatcgctct tggcgtggcg atgctcctgc
      481 tatgcagctt cgccgccggc atgcgttcgg caagcgttac ccagatcctg caatatgccg
      541 tgctgctcgc tgttgcggtg gtcgcactgg ggctgatggt gtggcagcag ggagcggtgt
      601 tctccggggc tgagccggcc ggcctcaaag cggcgctcgg cattctcaag ctcgacagct
      661 ttgccgcgga gggcagcctc aatcgcttgg cgctgctgtt ctgcctggtc gcgggggtcg
      721 catcgctgcc gcatctcttg atgcgaggtc tcgtcacgcc ttcgatcgag gaggcgcgaa
      781 cgtcgtttct ctgggctctg ccgttcgccg gcgtgcttct gctgcttgcc gcaccctttc
      841 tctcgctgtt cgaggccgcg ccgacgcaag agtcgcccgc cagcgccatc ctctcgttcg
      901 ggctggtcgc cgtgggcgcg atctccctcc tgcttgccgt cggcagcggc ctcgtgctcg
      961 ccatcgccaa tgcgctctcg tacgacctct attaccggag cctgcatctc agcgcctcga
     1021 cggagcggcg catcctcgtg gcccgcgcgg ccgtcgtgct caccgccgcc ctcgccgcag
     1081 gggtcacctt cgcggcgccg gacgtcattc cgctcatgac cggagcgtcg ttctcgctcg
     1141 cggcgagcgc gtttctgccc gcgctcctgc tcggcgtttg gtggaagcgc gcgacaggcg
     1201 agggggcgct ggccggcatg ctcgcgggcc tagcggtgtg tctctattac ctgctggcgc
     1261 cgcgctatat cccgttcgcc ttctacgaga cctcgagctt cctctcaaac gccacacccg
     1321 acgaggcggc gcattacgtc gcgctccggc aagggtatta cctcgccgat gcgtccgggc
     1381 gagaggcggc cttgtcgaca tggcgggcga tggcgcttca gctcgccaat tggggcggcg
     1441 tcaagcgcga gttcgccgcc ctgtttgccg tgccgctcgg ttttgcggtg atgatcgggg
     1501 tcagcctgtt tacgcccgcc ccgtcgcgcg atgtgcagag cttcgtcgag gatctgcgca
     1561 aaccgcaggt tgcctgaagg ctactgggcg ggcggattgg ccgccgattg cgccggtgcg
     1621 gcgctcgcgc caccgtcgag cacgtggaag gaatcgcgaa agcgttccgc ttcgtcgctg
     1681 gtggcgtggc tcttgggccc ggcgctgacc agcatataca cacggtcgcc ggcgggcacg
     1741 acatcgatca gatggctgag cttgctcttg ttgtcgtcag cgatcgcctc gaagccggag
     1801 cggccgtcga tggtcgccgt acttcgcttg cgcaagcgcg actcgctgcc cttggcatag
     1861 gcgttgacga gcttaacgaa tgcgtccgtg tccgggttcg gtcctttgcc ggccggatac
     1921 tcgaagacga cgacgctata gacggtgtcg tcgccgacgc tcacctggaa gtcgtattgg
     1981 atgagggggt tctggccttc gaccggtgcg gtcgtcgtct taggcgcccc ggggaatgat
     2041 acggagaagc cgcgctcggg cgattggaac tccgtccatt gcagctcttg cggtgccgcg
     2101 gcgggcgcat cctcggcgag agcgggcagc accgtcgtgg cgagcagcgc caagcccaac
     2161 gttgcgcttc ggatcgacat ccctcaagcc ctcctcatcg ccgtccgccg aatcggcatc
     2221 ttagccgatc cggctgcagg gttcacccag gcttgtcgcc gtccgcctaa ccctggggcg
     2281 aggatttcag cgcccgctca tacatggctt cgacatcctt tttcagggca cggcgcgaat
     2341 ccgggcggag at
//
LOCUS       DATNAE010000226         2213 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_225862, whole genome shotgun sequence.
ACCESSION   DATNAE010000226 DATNAE010000000
VERSION     DATNAE010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225862"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..946)
                     /locus_tag="VN518_05155"
     CDS             complement(<1..946)
                     /locus_tag="VN518_05155"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HXE03994.1"
                     /translation="MSLLANLALRQPRNIDASTLGRDAMAGGISAVVQIAYCISFAAL
                     IFTGDLASGFSLGLAGLIMGTVVTCVVIALTSTFSPVIGGPDSPAVAVMSVLAASIAT
                     ALAAKGAPTGQIVINVLVALSVSTLLTGILLYGVGALKMGQWLRFIPYPVIGGFLAAS
                     GILLITGGMEVVTQTNLTLSPSSWQVLYSSLYGPQILIGALFAASIPVLGRFVPNYLA
                     LPIAFFGFLLVLDVSLFGFFNDATIHSAWFLPSLGELTLWWPINAMIGQEVDWGVIAQ
                     SSAEIGSFCGVMAIALLLDVSSLEVARQKTSDLDQEFRS"
     gene            complement(1046..1411)
                     /locus_tag="VN518_05160"
     CDS             complement(1046..1411)
                     /locus_tag="VN518_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HXE03995.1"
                     /translation="MALAATDFQLLANAGFPPAKFKPGEVIFSEGDKGDKMYVIRSGE
                     VEVERDGKIVETLSGGGIFGEMALIDGSPRAATARAKTACEVAAITEKTFLFLVHETP
                     FFAIAVMRALADRLRRSAP"
     gene            complement(1493..>2213)
                     /locus_tag="VN518_05165"
     CDS             complement(1493..>2213)
                     /locus_tag="VN518_05165"
                     /inference="COORDINATES: protein motif:HMM:NF014801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HXE03996.1"
                     /translation="TFGTGCFVLANTGEEKVASATRMLSTVFHQLDGKRAYALEGAIF
                     MAGATVQWLRDNLGIIATAAESEALAREADPNSGVYLVPAFQGLGAPFWDAGARAAVL
                     GLTRAAAKSDLVAAGLEAVAFQTRDLLTAMVRDMAVSGIPMPSALRVDGGMTPNAWLM
                     QRLADILGQSVEVAATPETTALGAAYHAGQAVGFYGDAEELGKAWAPERVFAPKMSET
                     EREARYGGWLDAVARVRSQGV"
BASE COUNT          424 a          726 c          721 g          342 t
ORIGIN      
        1 tggagcgaaa ctcctggtcg agatcgctgg ttttctgccg cgccacttcg aggctcgaca
       61 cgtcgagcag gagcgcaatc gccatcacgc cgcagaatga gccgatctcg gcgctgctct
      121 gggcgatcac gccccaatcg acctcttggc cgatcatggc attgatcggc caccacaagg
      181 tcaactcgcc gaggctcggc aggaaccacg cgctgtgaat cgttgcatcg ttgaagaagc
      241 cgaacaggct cacgtcgaga acgaggagaa agccgaaaaa ggcgatcggc agagcgaggt
      301 agttcggcac gaatcgcccg agcaccggga tggaggcggc aaacagcgcc ccgatcagaa
      361 tctggggtcc gtaaagcgag gaatacagca cctgccaact ggatggcgac agcgtgagat
      421 tggtctgggt gacgacctcc atcccgccgg tgatgagcag gatgcccgaa gcggcaagaa
      481 agccgccgat caccgggtaa ggaatgaagc gcagccattg ccccatcttc agcgcgccga
      541 cgccgtagag caggatgccg gtcaaaagcg tgctgaccga cagcgcgacc agcacattga
      601 tgacgatctg cccggtcgga gcgcccttgg cggcgagtgc cgtggcgatc gaggcggcca
      661 gcacgctcat caccgcgacg gccggcgagt ccggcccgcc gatcaccgga gagaaggtcg
      721 aggtcagggc gatcaccacg caggtgacga cagtgcccat gatgagtccg gccaggccca
      781 gggagaagcc gctggcaaga tcgccggtga agatcagcgc ggcgaaggag atgcaatagg
      841 cgatctgcac caccgccgag atgccgccgg ccatcgcgtc ccgcccgagc gttgacgcgt
      901 cgatgttgcg cggctggcgc agcgcgagat tcgctagcag ggacaaggcc taatgcgcag
      961 cttggggttt agtcgcgccc gatcatggct ggcgcacgtc acaatggcaa gccggcaggc
     1021 ctaaagcacg gcgcaaaccc acggtttatg gggccgatct tcgcaagcga tcggctagcg
     1081 cgcgcattac ggcgatggcg aagaacggcg tttcgtgcac caggaacagg aaggttttct
     1141 cggtgatggc agccacctcg caagccgtct tggcccgcgc cgttgccgcc cgcggcgatc
     1201 cgtcgatcaa ggccatctcg ccgaagatcc cgccgcccga cagcgtctcg acgatcttgc
     1261 cgtcacgctc cacctccacc tcgcccgagc ggatcacgta catcttgtcg cccttgtcgc
     1321 cctccgaaaa gatcacctcg cccggcttga acttggcggg agggaagccg gcattggcga
     1381 gaagttggaa atcggttgca gctagcgcca tgtgctctgt cctccttggc cggcgggtgc
     1441 ggacggcaat ctatgacgat cctgtgacgg tactataaag gcgagcgggc cttcaaaccc
     1501 cttgcgatct cacccgcgcc accgcgtcga gccagccgcc atagcgtgcc tcgcgttcgg
     1561 tttcgctcat tttcggcgcg aacacccgct ccggcgccca ggctttgccg agctcttccg
     1621 catcgccgta gaagcccacc gcctggccgg cgtgataggc cgcgcccagc gcggtggtct
     1681 cgggcgtggc cgcgacttcg acgctctgcc cgaggatgtc ggcgagccgc tgcatgagcc
     1741 aagcgttcgg cgtcatgccg ccgtcgaccc gcaaggccga gggcatggga atgccgctca
     1801 ccgccatgtc gcggaccatg gcggtcagca gatcgcgcgt ctggaaggca acggcctcga
     1861 gccctgccgc gaccagatcg gacttagccg ccgcgcgcgt caatccgaga acggcggcgc
     1921 gcgcgcccgc gtcccagaac ggcgcgccca gtccctgaaa ggcgggaacg agatagaccc
     1981 ccgagttcgg gtctgcctcc cgcgccaagg cctcgctttc tgcggcagtg gcgatgatgc
     2041 cgagattatc ccgcagccat tgcaccgtcg cgcctgccat gaagatggcg ccttccagcg
     2101 cgtaagcgcg cttgccgtcg agctgatgaa agacggtcga gagcatgcgg gtggccgagg
     2161 ccactttctc ctcgccggtg ttggcgagca cgaagcagcc ggtgccgaag gtc
//
LOCUS       DATNAE010000227         3509 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3231359, whole genome shotgun sequence.
ACCESSION   DATNAE010000227 DATNAE010000000
VERSION     DATNAE010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3231359"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..168)
                     /locus_tag="VN518_05170"
     CDS             complement(<1..168)
                     /locus_tag="VN518_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006023331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HXE03997.1"
                     /translation="MQRVEAALAGEMERQLPAGLYLVATPIGNLADISLRALAVLTRA
                     DLIAAEDTRHSR"
     gene            475..1659
                     /locus_tag="VN518_05175"
     CDS             475..1659
                     /locus_tag="VN518_05175"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HXE03998.1"
                     /translation="MRLPLGIGLTLRAAKVLLAGFAVSMLLAACGGGSGPGPISPGVT
                     GSTGAVKVALLLPLTGSGNTPSVAKALKQAAELALFDFDNPSVSLVPKDTKGTPAGAQ
                     AAAQSAIQDGAELIIGPLFAQEVAAAAPIARQSNIPMIAFSSDEKVAGGGVYLLSFLA
                     GRDVPRIVSYAASRGKHNFALLIPQSPYGRLAEEAFARSMGRSGGQAVIRATFPPNDA
                     NAMLGPVRQVANAMKAGQADALFLPAGPEDLQSLAPLLASNGVTSKGLQFLGTGQWDY
                     PSIGRETALVGGWFPAPDPKGWSDFVGKYAKTYGSTPPRIASLGYDAVSLAVSLSPNP
                     PGQRYTATTLTRGSGFAGVDGLFRLLPDGTSERGLAILEVREYGPQVMEPAPNNFTSA
                     QY"
     gene            complement(1673..2815)
                     /gene="hemW"
                     /locus_tag="VN518_05180"
     CDS             complement(1673..2815)
                     /gene="hemW"
                     /locus_tag="VN518_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HXE03999.1"
                     /translation="MTEPFGVYVHWPFCLAKCPYCDFNSHVRHGGIDQGDFVTAYLSE
                     LNHFASLAPGRIVSSIFFGGGTPSLMAPETVGTILDRIAELWTVAPDAEITLEGNPTS
                     VEAGRFAGYRAAGVNRVSLGVQSLDDASLKALGRKHTSEEALAALAIAKRNFERVSFD
                     LIYARQGQKLEDWREELTRALIHAQDHLSLYQLTIEPGTPFATLHAAGALRVPEGEQA
                     EEFYLLTQELCDAAGLPSYEVSNHARPGAESRHNLLYWRGHDYAGVGPGAHSRITRDD
                     GKHAIAIRKSPEAWLQAVAASGHGIEQDERLSPQNSADEYLLMGLRLAEGIDVTRLKA
                     IDGRKLDDARLQQLEAQGLVKRTPGRLRATDTGRLVLDRLIVELAA"
     gene            complement(2808..3419)
                     /gene="rdgB"
                     /locus_tag="VN518_05185"
     CDS             complement(2808..3419)
                     /gene="rdgB"
                     /locus_tag="VN518_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HXE04000.1"
                     /translation="MGVTLRRGTRLVIASHNEGKVREIADLLAPFGVEAVSAGKLGLP
                     EPEETGDSFVENARLKAAAAARDSRLPALADDSGLEVKALGGEPGIHSARWGGAARDF
                     GLAMERVHRELAAAHAKDSTANFTCALALASPDGKSEVFEGKVFGTLTWPQRGARGFG
                     YDPIFVPEGYKQTFGEMEPSLKDSISHRARAFEKLMLAAFDDD"
BASE COUNT          577 a         1119 c         1192 g          621 t
ORIGIN      
        1 gcggctgtgg cgcgtatctt ccgccgcgat caggtcggcg cgggtgagaa cggcgagagc
       61 gcgaagcgaa atgtcggcga ggttgccgat cggcgtcgcc acgagataga ggccggcggg
      121 aagctgccgc tccatttcgc cggcgagcgc ggcttcaacg cgctgaatcc tgcgcgctgc
      181 gtcttggtcg tcgtggcgat gatcctgcac gcatgcgctt atagctcagg caggctgccg
      241 gtgaactgcc taaagtgcgg gcttgagcgg cgctatgcta gaagaggggc catgctatag
      301 ttgcgcttcg cgcccaccac gaatgagcca tagttgcggt cgaaattttg tccaaagggc
      361 ggacggcggc ggtttttgca gggccgatgg acgaaggtaa gcttgcgtct gccgctcggg
      421 gacacaaggc ctcgcgatag cctcgcgctt cgctcgcctt gaagcgaagg ggtcatgcga
      481 cttccacttg ggattggtct gacgcttcgc gccgcgaagg ttctgctcgc gggctttgcc
      541 gtttcgatgc tgctcgccgc ttgcggcggc ggcagcggcc cagggccgat cagcccgggc
      601 gtgaccggct cgaccggcgc ggtgaaggtc gctctgctcc tgccgctcac cggatcgggc
      661 aacacgccct cagtggccaa ggcgctgaag caggcggccg agctcgcgct gttcgatttc
      721 gacaatccgt cggtctcgct cgtgccgaaa gacaccaagg gcacgccggc gggcgcgcaa
      781 gcggcggcgc agagtgcgat ccaggacggc gccgagctga tcatcgggcc gctcttcgcg
      841 caagaagtcg ctgccgccgc tccgatagcg cgccagtcga acattcccat gatcgccttt
      901 tccagcgacg agaaggtcgc gggcggcggc gtctatcttt tgagcttcct cgccggacgc
      961 gacgtgccgc gcatcgtttc ctacgccgcc tcccgcggca aacacaactt cgcgctgctg
     1021 atcccgcagt cgccttacgg caggctggcc gaggaagcgt ttgcgcgcag catggggcgg
     1081 agcggggggc aggccgtgat acgcgccacc ttcccgccga acgacgccaa cgccatgctc
     1141 ggcccggtgc ggcaggtagc aaacgccatg aaggccgggc aggcggatgc gctgttcttg
     1201 cccgcggggc ctgaggacct gcaatcgctc gcgccgctcc ttgcctcgaa cggcgtgacc
     1261 agcaaaggcc tgcaattcct cggcaccggg caatgggact atccctcgat cggcagggag
     1321 acggctctgg tcggcggctg gttcccggca cctgacccga aaggctggag cgatttcgtc
     1381 ggcaaatacg ccaagaccta tggctcgacc ccgccgcgca tcgcgagcct cggctatgac
     1441 gcggtgagcc tcgccgtgtc gctgtcgcca aatccgccgg ggcagcgtta cacggcaacg
     1501 acgctgacgc ggggaagcgg ctttgccggc gtcgatggat tgttcaggct gctgcccgac
     1561 ggcacatcgg agcgcgggct cgctattctc gaagtgcgcg aatacgggcc gcaggtaatg
     1621 gagcctgcgc cgaacaactt cacatccgcg caatattagg cgcttgcccc agtcaagcgg
     1681 cgagctcgac gatcagccgg tcgagcacga ggcggccggt gtcggtggcc cgcagccgtc
     1741 cgggggtccg cttgaccagg ccttgcgcct cgagctgttg cagccgcgca tcgtcgagct
     1801 tccgcccatc gatggctttg agccgcgtca cgtcgatgcc ttcggcaagg cgcaagccca
     1861 tgagcagata ttcgtcggcg gagttttgcg gactcaagcg ctcgtcctgc tcgatgccgt
     1921 gacctgaggc ggcgaccgct tgcagccagg cctccgggga tttgcggatg gcgatggcat
     1981 gcttgccgtc gtctcgggtg atgcggctgt gggcgcctgg gccgacgccg gcatagtcgt
     2041 ggccgcgcca ataaaggaga ttgtgccgcg actccgcgcc aggcctcgcg tggttcgaaa
     2101 cttcgtagga ggggaggcct gcggcatcgc acagctcctg ggtgaggaga taaaattctt
     2161 cggcctgctc gccctccggc accctcagcg cgccagctgc gtgtagcgtc gcgaacggcg
     2221 tgcccggctc gatggtaagc tgatagaggg agaggtggtc ctgggcgtgg atcagtgcgc
     2281 gggtgagctc ctcgcgccag tcctcaagct tctgcccctg ccgtgcatag atcaggtcga
     2341 acgagacgcg ctcgaaattg cgcttggcga tggcgagcgc ggcaagggcc tcttcgcttg
     2401 tgtgcttgcg gccgagcgct ttcagactcg catcgtcgag cgattgcacg ccgagggaga
     2461 cgcggttgac gcctgcggcg cggtagccgg cgaagcggcc ggcctcgacg gatgtcggat
     2521 tgccttcgag cgtgatctcg gcgtcagggg cgacggtcca tagctcggcg atgcggtcga
     2581 ggatggtgcc gacggtctcg ggcgccatca gcgatggcgt gccgccgccg aaaaaaatgc
     2641 tcgagacgat gcgtcccggc gccagcgatg cgaagtggtt cagctccgaa agataagcgg
     2701 tgacgaagtc accctggtcg atgccgccgt gacggacatg cgagttgaag tcgcaataag
     2761 ggcatttggc caggcagaac ggccaatgca cataaacccc gaatggctca gtcatcgtca
     2821 aaggcggcca gcatcagttt ctcgaaggcg cgggcgcggt gtgagatcga gtctttcaag
     2881 ctcggctcca tctccccgaa tgtctgttta tagccttccg gcacgaagat cggatcatag
     2941 ccgaaaccgc gggcgccgcg ttgcggccac gtgagcgtgc cgaacacctt gccttcgaac
     3001 acctccgact tgccgtcggg cgaagcaagc gccagcgcgc aggtgaaatt ggcggtcgag
     3061 tccttggcat gggcagcagc gagctcgcga tgcacgcgct ccatggcgag cccgaaatct
     3121 cttgctgctc cgccccagcg cgccgaatgg atgcctggct ctccgccaag tgccttgacc
     3181 tccagtcctg aatcgtcggc gagcgccggc agccgtgagt ctcgcgctgc ggccgccgcc
     3241 ttcaatctgg cattctcgac gaagctatcg ccggtctcct caggctcagg cagtccgagc
     3301 ttgcctgcgg acaccgcctc gacgccgaat ggcgcgagca gatcggcgat ctcgcgcacc
     3361 ttgccttcat tgtggctggc gatgacgagg cgcgtccccc gccgcaaggt tacgcccatc
     3421 gtcaggcgac ggtgagcttc tgcagttgca cgagctcggc gatgccgcgc ttcgccagag
     3481 ccatcagctg atcgaattgc gcctggctg
//
LOCUS       DATNAE010000228         3677 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_4437090, whole genome shotgun sequence.
ACCESSION   DATNAE010000228 DATNAE010000000
VERSION     DATNAE010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4437090"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..841
                     /locus_tag="VN518_05190"
     CDS             <1..841
                     /locus_tag="VN518_05190"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HXE04001.1"
                     /translation="LGQSLSTLIWVGPEDQLTLTENAQPALMAVSLAVIRVLEAEKGL
                     SLKESVTFVAGHSLGEYSALAAAGSLSLADTARLLKTRGQAMQAAVPVGEGAMAALLG
                     ADLSQAQELAKAASEGEVCAAANDNAPGQVVISGTRTAIERAVALAPKFGARRAVLLP
                     VSAPFHCALMQPAAQVMQEALSKVKISAPSVPLVANVLASAVSDPEAIRARLIEQVTG
                     MVRWRESMLYLRDQGVDSLYEIGAGRVLTGLARRFEGFEARSVGTPEELEAAATPLQA
                     RNA"
     gene            857..1594
                     /gene="fabG"
                     /locus_tag="VN518_05195"
     CDS             857..1594
                     /gene="fabG"
                     /locus_tag="VN518_05195"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HXE04002.1"
                     /translation="MFDLTGKGALVTGATGGIGWAIAEALHAQGATVAISGTRAERLN
                     ELAAKLGSRVAALPCDLRDRAAVVKLAEDAEKALGQVDILVNNAGITHDNLFMRMKDE
                     EWDDVIAVNLTSVFVLTRGVLRGMMRRRAGRIVNIASISGVLGNPGQGNYAASKAGLV
                     GMTKSLAREVSSRGITANCIAPGFISTPMTDALTPKQTEAIAAAIPAAKFGKPEDIAA
                     VVVFLASQEAGYITGETVHVNGGMVMV"
     gene            1856..2095
                     /locus_tag="VN518_05200"
     CDS             1856..2095
                     /locus_tag="VN518_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008130699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HXE04003.1"
                     /translation="MSDISERVKKIVVEHLGVETDKVTDSASFIDDLGADSLDTVELV
                     MAFEEEFGCEIPDDAAETILTVGDAIKFLEKNTAA"
     gene            2199..3464
                     /gene="fabF"
                     /locus_tag="VN518_05205"
     CDS             2199..3464
                     /gene="fabF"
                     /locus_tag="VN518_05205"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HXE04004.1"
                     /translation="MRRVVITGLGLLSPLGCGAEVTWQRLTAGENAAKRVENFDVSDL
                     TCQVACQIPRGDGSDGTFNPNDWMEPKEQRKVDDFIIYAMAAATQALADAGWKPTAYE
                     DQIRTGVLIGSGIGGLQGIEKTSLLFAEKGPRRVSPFFIPGRLINLAAGYVSIQHGLK
                     GPNHAVVTACSTGAHAIGDASRLVGLGDADVMLAGGAESPVCRLSIAGFAACRALSTN
                     FNDQPQRASRPYDRDRDGFVMGEGAGVVVVEALEHAKARGAKIYAEIIGYGLSGDAFH
                     ITAPAEDGDGAYRCMVAAVKRAGIEPADIDYINAHGTSTPMGDEIELAAVERLFGNAA
                     GKVSMSSTKSAIGHLLGAAGAVEAIFSALSIRDNLAPPTLNLDNPSVATAIDLVPHKA
                     HRRTIDTVLSNSFGFGGTNASLILRRCDA"
BASE COUNT          651 a         1194 c         1183 g          649 t
ORIGIN      
        1 cctcggtcag tcgctctcga ccttgatctg ggtgggacct gaggatcagc tcaccctgac
       61 cgagaacgcc cagcccgcct tgatggcggt ctcgcttgcc gtcattcgcg tgttggaggc
      121 cgagaaagga ctctcactca aggaaagcgt caccttcgtg gccggccatt cgctcggcga
      181 atattcggcg ctcgccgccg ctggaagctt gagccttgcc gacaccgcgc gcctgctcaa
      241 gacgagaggg caggccatgc aggcggcggt cccggtgggc gagggagcga tggcagccct
      301 gctcggggct gatctgtcgc aggcgcaaga gcttgccaag gcggcaagcg agggcgaggt
      361 ctgcgccgct gccaacgaca atgcccctgg ccaggtggtg atcagcggca cgcggacggc
      421 gatcgagcgg gccgtcgcgc tcgccccgaa attcggcgct cgccgcgccg tgctgcttcc
      481 ggttagcgct ccctttcatt gcgcgctgat gcagccagct gcccaagtca tgcaggaggc
      541 tctgagcaag gtgaagatct cggccccttc ggtgcctctc gtcgccaatg tgctcgcctc
      601 cgccgtcagc gacccggagg ccatccgcgc ccgtctcatc gagcaggtga ccggcatggt
      661 gcggtggcgt gagtcgatgc tctatctgcg cgaccagggc gtcgatagtc tttatgagat
      721 tggcgccggc cgcgtgctca cgggacttgc acgccggttc gaaggtttcg aggcgcgctc
      781 cgtgggcacg cccgaggagc tcgaagctgc cgccacgccg cttcaggccc gtaacgcgta
      841 acaggaggag tgcttcatgt tcgatttgac cggcaagggc gcgcttgtca ccggtgccac
      901 cggaggcatc ggctgggcga tcgccgaggc gttgcatgcg caaggcgcca cggtggcgat
      961 ctccggcacc cgcgccgaac gcctcaacga gcttgccgcc aagcttggct cgcgcgtcgc
     1021 ggcgttacct tgcgatttgc gcgaccgtgc ggccgtagtg aagctcgccg aggacgccga
     1081 gaaggctctc ggccaggtcg acattctcgt caacaatgcc ggtatcaccc acgacaattt
     1141 gttcatgcgc atgaaggatg aggaatggga cgatgtcatc gccgtcaatc tcacctccgt
     1201 gttcgtactc acccgcggcg tgttgcgcgg catgatgcgg cgccgggcgg ggcgtatcgt
     1261 caacatcgcc tcgatctccg gcgtgctcgg caacccgggg caggggaact atgccgcctc
     1321 aaaagctgga ctggtcggca tgaccaaatc gctggcccgc gaggtgtcga gccgcggcat
     1381 caccgccaat tgcatcgctc cgggcttcat ctccactccc atgaccgacg cgctgacgcc
     1441 taagcagacc gaggcaatcg ccgcggcaat tccggctgcc aagttcggaa aacctgagga
     1501 tatcgcggcg gtggtggtgt tcctggcaag ccaggaggcc ggctacatca ccggcgagac
     1561 ggtgcatgtg aatggcggca tggtgatggt ttgagccctt gtgcagcaag cccttggcag
     1621 ggcggccgga gcggcctcgg caggctcgat tgggcctgtt cagccctgct ggcaaaggcc
     1681 gggaaagtgt gttacccaaa cggccgctct gtgaagctct atgggggctt gacaaagaca
     1741 atcctcctcg cgttcaaacc ctgtgacttg ccgctctagg gtgcgttagg ccgtgagccg
     1801 agagaccggg ggacgtgcga ggcctaagga cgaagaagga aacggtgagg cggacatgag
     1861 cgatatctcg gagcgcgtaa agaagatcgt agtggagcat ctcggcgtcg agaccgacaa
     1921 ggtcaccgac agtgcgagct tcatcgacga tcttggggcc gacagcctcg acaccgtcga
     1981 gctggttatg gccttcgagg aagagtttgg ctgcgagatc cctgacgatg ccgcagaaac
     2041 tattctgacc gtgggcgacg ccatcaagtt cctggagaag aacacggccg cctgaccctt
     2101 cttgctcgtc gcgcgctcga gccgtccagc gtcgttgtaa gctttaggcc atacggctcg
     2161 atcaccgatt ctctacgagg atcacgcgaa agcgtcagat gcgacgtgtc gtcatcacag
     2221 gtcttggtct cctttcaccg ctcggctgcg gcgccgaagt cacctggcaa aggctgaccg
     2281 ccggcgagaa tgcggccaag cgcgtcgaga atttcgacgt ctccgacctg acctgccagg
     2341 tcgcctgcca gatcccgcgc ggcgacggca gcgacggcac cttcaaccca aacgactgga
     2401 tggagccgaa ggaacagcgc aaggtcgatg actttattat ctatgccatg gccgcggcca
     2461 cccaggccct ggccgacgca ggctggaagc ccaccgccta tgaggatcaa atccgcactg
     2521 gcgtgctgat cggctcgggc atcggcggac tgcaaggcat cgagaagacc tcgctcctct
     2581 tcgccgagaa ggggccaagg cgggtaagcc cgttcttcat cccgggacgc ctgatcaatc
     2641 tcgctgccgg ctatgtctcg atccagcatg ggctgaaagg tccgaaccat gccgtggtca
     2701 ccgcctgttc tacgggtgcg cacgccatcg gcgatgcctc gcggctggtc gggctcggcg
     2761 acgccgacgt gatgctcgcc ggaggtgccg aatcgccggt gtgccgcttg tccattgccg
     2821 gcttcgcagc ctgccgggcg ctgtccacca acttcaacga tcagccgcag cgcgcctcgc
     2881 gtccctacga tcgcgatcgc gacggtttcg tcatgggcga gggcgccggc gtggtggtgg
     2941 tcgaggcgct tgagcacgct aaggcgcgcg gcgccaagat ttatgccgag atcatcggct
     3001 acgggctttc cggcgacgcc ttccacatca cggcgcccgc cgaggacggc gacggcgcct
     3061 atcgctgcat ggtcgcggcg gtaaagcgcg ccggcatcga gccagccgac atcgactaca
     3121 tcaacgccca tggcacctcg acgccaatgg gcgacgagat cgagcttgcc gccgtcgaac
     3181 ggctgttcgg caatgccgcc ggcaaggtct ccatgtcatc gaccaaatcg gcgattggcc
     3241 atcttctcgg cgctgccggc gcggtcgagg cgatcttctc ggcgctgtcg atcagagaca
     3301 accttgcgcc gcctaccctc aatctcgaca atccctcggt ggcgacggcc atcgacctcg
     3361 tgccgcacaa ggctcaccgg cggacgatcg acactgtcct gtccaactcc ttcggattcg
     3421 gcggcaccaa tgcctcgctg atcctgcgac ggtgcgacgc ctgatcgagg ccgctcgtcc
     3481 cttgccagcc gaacctccct ttcgccatat tctcacgcca agtggataag cagttcccgc
     3541 gcggggcatt aaggcaatgt ccgtgaccag cgactcctcg tcgacggcga gcgacaacga
     3601 tgcgtgcgag cgtgcggaac gcgcgatgcg gaatttcgac acgccgccac gcagccccta
     3661 cgaagcgctc gagcccg
//
LOCUS       DATNAE010000229         2220 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2940864, whole genome shotgun sequence.
ACCESSION   DATNAE010000229 DATNAE010000000
VERSION     DATNAE010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2940864"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="VN518_05210"
     CDS             complement(<1..449)
                     /locus_tag="VN518_05210"
                     /inference="COORDINATES: protein motif:HMM:NF015699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HXE04005.1"
                     /translation="MTLKSMTGFARADGVHGDTSWHWEARSVNGRNLDLRLRLPSGFE
                     GLEIRARGLAQEKLARGNCTINLWVKRESGQMEIRLNEAALGQARAVAARAHDLTGLK
                     PPRLDTLLAMRGVVETVEGEETEEAQAALYHALLASLASALDQLIAAR"
     gene            complement(563..2041)
                     /gene="mltG"
                     /locus_tag="VN518_05215"
     CDS             complement(563..2041)
                     /gene="mltG"
                     /locus_tag="VN518_05215"
                     /inference="COORDINATES: protein motif:HMM:NF014656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HXE04006.1"
                     /translation="MSLTRDFQATANDNDDRIQAGPAGRQLAVLPRSPTEALQPMRPP
                     EPPSHDEPEPDPHPFLRMLDGLVTFFFLLACLAVGVFFWVKMQFDQPGPLATSTVFVI
                     PKGEGVSAIAERLERDGVIDDRRVFMTSILYFMYLKGQGSLKAGEYEFGKSASMRQVL
                     DTLVEGKSIEHKVTLAEGLTSYQIVQKLMAQTELHGEITEIPPEGSLLPDTYRFGRND
                     TRQDIIERMQAAHKKFLAKVWETRDPEIVVTTPEEAVILASIVEKETGRADERPLIAS
                     VFENRLRKNMRLQSDPTIIYGLVGGKGALDHPIQQEELDRETGYNTYKINGLPPTAID
                     NPGRASIEAVLRPAKTKDLYFVADGTGGHVFAASLEEHNKNVFKWRKVEREMRAKEAQ
                     EAAEKAAQEATAGGAPDTGATPGAPVGAAALPREAGAPIAKTAPGALAADPSITSGTL
                     DPDAMMNAQDPFAVPAEAGGGEASGADAAGEAIPKPLRNPKR"
BASE COUNT          354 a          736 c          708 g          422 t
ORIGIN      
        1 cgcgcggcga tgagttgatc cagcgccgat gcgaggctcg cgagcagcgc atgatagagc
       61 gccgcttgcg cctcctcagt ctcctcccct tccaccgtct cgaccacgcc gcgcatggcg
      121 agcagggtgt cgagacgcgg cggcttcagg ccggtcagat cgtgcgcccg cgccgccacg
      181 gcgcgcgctt ggccgagcgc ggcctcgttc agcctgatct ccatctgccc gctctcgcgc
      241 ttgacccaga gattgatggt gcaattgccg cgcgcgagtt tttcctgcgc caggccgcgc
      301 gccctgatct ccagcccctc gaagcctgac gggagtcgca agcgaagatc gagattgcgg
      361 ccgttgacgc tgcgcgcttc ccaatgccag ctcgtgtcgc cgtgcacgcc gtcggcgcgg
      421 gcaaagccag tcatgctctt cagcgtcatc cggtcgccag ccttgccgcg ttgggtcgtc
      481 aggtcatgca gcttataagc cgagtcgcgc gtctacggca cggctaggct ggcagcttag
      541 tgaagatcgg ctgcaggcgc gcctagcgct tcggattgcg caacggcttc ggaatcgcct
      601 cccccgccgc gtccgcacca gaggcttcgc caccaccggc ctcggccggc accgcgaagg
      661 gatcctgggc attcatcatc gcatccgggt ccaatgtgcc cgaagtgatc gaaggatcgg
      721 cggcaagggc tcccggagcg gtcttggcaa tcggcgcgcc cgcctcgcgt ggcagcgccg
      781 cggctccaac cggagcgcca ggcgtcgcgc ccgtatcggg ggcaccgccg gcggtcgcct
      841 cctgcgccgc tttttcggcc gcctcctgcg cttccttggc gcgcatttcg cgctcgacct
      901 tccgccactt gaagacattc ttattgtgct cttcgagcga ggccgcgaag acgtggccgc
      961 cggtcccgtc ggcgacgaaa tagaggtctt tggtcttggc cggccgcagc accgcctcga
     1021 tcgaggcgcg ccccggattg tcgatcgcgg ttggcggtaa gccgttgatc ttataggtgt
     1081 tatatccagt ctcgcggtcg agctcttctt gttggatggg atgatcgagc gcgcccttcc
     1141 cgcccaccag tccatagatg atggtggggt cggattgcag ccgcatattc ttgcgcaagc
     1201 ggttctcgaa cacgctggca atgagcggcc gctcgtcggc ccgcccggtc tccttctcga
     1261 cgatggaggc gaggatcacc gcctcttccg gcgtcgtcac cacgatctcg gggtcgcgcg
     1321 tctcccacac tttggcgaga aacttcttat gcgcggcctg catgcgctcg atgatgtcct
     1381 ggcgggtatc gttgcggccg aatctataag tgtcggggag caggctgccc tcgggcggaa
     1441 tctcagtgat ttcaccgtga agctcggttt gcgccatgag cttctgcacg atctggtagc
     1501 tggtcagccc ctcggccagc gtcaccttgt gctcgatcga tttgccctcg accagcgtat
     1561 cgagcacttg ccgcatgctc gcactcttgc cgaactcgta ttccccggct ttcaggctcc
     1621 cctgcccctt cagatacata aaatagagaa tgctggtcat gaacacgcgg cggtcgtcga
     1681 tcacgccgtc gcgctccagc cgctcggcga tggcgctcac gccttcgccc ttgggaatga
     1741 cgaacacggt cgaggtggcg agcggccccg gctggtcgaa ttgcatcttc acccaaaaga
     1801 agacgccgac ggcgaggcag gccagcagga agaagaacgt gaccaagccg tcgagcatgc
     1861 gcaggaaggg atgcggatcg ggctcgggct catcgtggct aggcggctcg ggcgggcgca
     1921 tcggctgaag cgcctcggtc gggctgcgcg gcaacacggc gagctgccgt ccagccgggc
     1981 cggcctggat ccggtcgtcg ttatcgttcg ccgtggcttg aaagtccctg gtcaaagaca
     2041 tggccaacac gccccacgcg caacgattcc atgcacttga agtcgaaata tggcgaaagg
     2101 gaggttgtca aaaggggggc gtcacacgtc gtaacggcgc aggatcagcg aggcattggt
     2161 cccgccaaat ccgaacgagt tcgacagcac cgtgtcgatg ctgcggcgcc tcgcctgatg
//
LOCUS       DATNAE010000230         4229 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_829577, whole genome shotgun sequence.
ACCESSION   DATNAE010000230 DATNAE010000000
VERSION     DATNAE010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4229
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_829577"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..864
                     /locus_tag="VN518_05220"
     CDS             157..864
                     /locus_tag="VN518_05220"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HXE04007.1"
                     /translation="MTLQRKTAFLALLIFAALPANSLSADEAPKRTISLAASGAIKTT
                     PDKVDISTGVTAQAPTAKEALAKNSAAMTEVIAALKGEGLDLKDIQTTNFNVQPLYEE
                     RKDGRAPKIVGYQVTNSVRITVRDVGKLGQILDKVVTLGATDVNAIEFGVAGPEALKD
                     EARKLAMRNAIANAKLYAEAAGAKLGKVLTISEDQGIIVARSMAPAPMEMAAKAVPIE
                     AGTATVEARVSVTFELE"
     gene            936..2999
                     /locus_tag="VN518_05225"
     CDS             936..2999
                     /locus_tag="VN518_05225"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015008727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HXE04008.1"
                     /translation="MTVQRAEVLDSAYAHERAVMISRHLRARGIEDPLLLAAMGAVPR
                     EAFVPEHLQEFSYEDSALPIEAGQTISQPYIVARMIELAEIKPGDKVLEVGAGSGYAA
                     AVMSRMANRVFAIERHKELAMLARSRLKRLGYGNAEIIIADGTKGLAKEAPFQAIIVS
                     AGGPHVPEALKQQLAVGGRLIIPVGEFGYQTLMRVRRTGEDSFEEEDFGAVAFVPLIG
                     EQGWAEPERERVKEAPVDAEPTGFAAGPLLPAKRALTIQNRLSRLMAETAEPFGDLHE
                     LCGLVERFSDRRVVMLGEATHGTAEFYQARARITEMLVDRHGFNIVAVEADWPDAAVY
                     DAFVRGLPRPVLPQSVFTRFPTWMWRNGQVADFLDRLKEINATIADPDRKAGFYGLDI
                     YSLGASIEAVLHYLDRVDQQAARVARERYGCLVPWRAEPARYGRMALSRGYAVCEKPV
                     ADALTDLLRKRLDYLTKDGEAFFDAEQNARIVAGAEQYYRAIYYGDAASWNLRDQHMF
                     DTLDRLLAERGPEAKAVVWAHNSHIGNAAFTEMGQVRGEHNIGQLARQRFGEDAALIG
                     FGTDRGTVAAASDWDGDMEIKRVRPARDDSYEGRSRDTGIAAFLLETGPGQRETVRSE
                     LAEPLLERAIGVIYRPETELLSHYFQAELSRQFDAWIWFAETQAVAARPVHAHGPDET
                     YPFGL"
     gene            3119..>4229
                     /locus_tag="VN518_05230"
     CDS             3119..>4229
                     /locus_tag="VN518_05230"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXE04009.1"
                     /translation="MKTGWLLTLVVAAAAGAGAYYYWHQQGPGRLDIQTAEVKRGDVR
                     RVVSTSGTVSALVTVDIGSQLSGNIGAVNVDYSSEVKQGEVLARIEPSSFETRVREEE
                     AAVAVAKANVDLQQAGIGRAEANLHKAELDLGRAKELVAKGATSQAALDTAIAAEQGA
                     IADLAIAKANVENGKATLTQHEATLDSARIDLGRTYIRSPIDGVVIDKIVEVGQTVAA
                     SLQAPKLFTIAQDLNQVQIQAQVDEADIGQVTSGNPVSFTVDAYPDVTFEGKVEQIRL
                     APISLQNVVTYTVVIAADNQLGRLLPGMTANVEIITGEHPGVVVVPNDALRFQPRGPA
                     EALISDATATVSIPTASAAGERTNRLLDRLKVDLEL"
BASE COUNT          760 a         1409 c         1346 g          714 t
ORIGIN      
        1 cggctctaac cagttgagct acgggcccca attcctccgc gtgattccgc ctcattagca
       61 gagttctatc cttacgcgaa acggctcggg tcagggcagt cgcgcgtcag cctccccgcg
      121 gcgcgccgct gaccgtcacc ggttgagagg actcccatga cgcttcaacg caagaccgcc
      181 ttcctcgctt tgctcatctt cgccgccctc cccgccaatt cgctcagcgc cgacgaggcg
      241 ccgaagcgca cgatctcgct tgccgcaagc ggcgcgataa agaccacgcc cgacaaggtc
      301 gacatctcga ccggcgtcac ggcccaggcg ccgaccgcca aagaggcgct cgccaagaat
      361 agcgccgcca tgaccgaggt gattgccgcg ctgaaaggcg aaggcttgga cctcaaggac
      421 atccagacca cgaatttcaa cgtgcagccg ctctatgaag agcggaagga tggacgggcg
      481 ccgaagatcg tgggctatca agtcaccaat tcggtccgca tcaccgtgcg cgacgtgggc
      541 aagcttggcc agatcctcga caaagtggtg acgctcggcg ccaccgacgt gaacgcaatc
      601 gagttcggcg tggctgggcc ggaagcgctc aaggacgaag cgcgcaagct tgccatgcgc
      661 aacgccatcg ccaatgccaa gctctatgcc gaggcggccg gcgccaagct cggaaaggtc
      721 ctgaccatca gcgaagacca gggcatcatc gtcgcccgct ctatggcgcc ggctcccatg
      781 gagatggccg ccaaggccgt tcccatcgag gccggaaccg ccacggtcga ggcccgcgtc
      841 agcgtcactt tcgagctgga ataggcgccg tcgaggcgcc gtgatatgat ccaaatcaag
      901 gccgaaccag gctcgcttca ggtagttttg tctccatgac cgtgcaacgc gccgaggtct
      961 tggacagcgc ctacgcgcat gagcgggcgg tgatgatttc ccgccacctt cgcgcccgcg
     1021 gcatcgagga tccactgctt cttgccgcca tgggcgccgt gccgcgcgag gccttcgtgc
     1081 ctgagcatct gcaggaattc tcctatgagg attccgcgct gccgatcgaa gccggccaga
     1141 ccatctcgca gccttacatc gtcgcgcgca tgatcgagct cgccgagatc aagccgggcg
     1201 acaaggtgct cgaggtcggc gccggctcag gctacgccgc ggcggtgatg agccgcatgg
     1261 ccaacagggt gttcgccatc gagcgtcaca aggagctggc catgcttgcc cgcagccggc
     1321 tgaagcggct cggctatggc aatgccgaga tcattatcgc cgacggcacc aagggcttgg
     1381 cgaaagaagc gcccttccag gcgatcatcg tctcggccgg cggtccgcat gtccccgaag
     1441 ctttgaagca gcagcttgcc gtcggcggcc ggctgatcat ccccgtcgga gaattcggct
     1501 atcagacgct gatgcgcgtt cgccgcaccg gggaggacag cttcgaggag gaggatttcg
     1561 gcgccgtggc cttcgtccct ctgatcggcg agcaaggctg ggccgagcct gagcgcgagc
     1621 gggtcaagga agcgccggtc gacgccgagc ccaccggctt cgccgcagga ccgcttctgc
     1681 cggccaagcg cgcgctaacc attcagaatc gcttgagccg gctcatggcg gagaccgccg
     1741 agccgttcgg cgatctgcac gaactctgtg gtctcgtcga gcggttcagc gaccgcaggg
     1801 tcgttatgct cggtgaagcg acccacggca cagcggagtt ctaccaggcg cgggcgcgca
     1861 tcaccgagat gctggtcgac cgccatggct tcaacattgt cgccgtcgag gccgactggc
     1921 ctgatgctgc ggtctacgac gccttcgtgc gcggcctgcc ccgtccggtg ctgccgcaat
     1981 ccgtctttac ccgcttcccg acttggatgt ggcgtaacgg acaggtggca gacttcctcg
     2041 atcggttgaa ggagatcaac gccacgatcg ccgaccccga tcgcaaggct ggcttctacg
     2101 ggctcgatat ctatagcctc ggcgcctcga tcgaggcggt gttgcattat ctcgaccggg
     2161 tcgatcagca agccgcccgg gtcgcgcgtg aacgctacgg ctgcctcgtc ccttggcgcg
     2221 ccgagcccgc ccgctacggc cgcatggcgc tgtcgcgcgg ctacgccgtt tgcgagaagc
     2281 cagtcgccga tgcgctcacc gatcttttgc gcaagcggct cgactatctc accaaggatg
     2341 gcgaggcctt tttcgatgcg gagcaaaatg cgcgcatcgt ggccggcgcc gagcaatatt
     2401 accgcgccat ctattacggc gatgcggctt cgtggaatct gcgcgaccag catatgttcg
     2461 acacgcttga ccgcctgctc gcagagcgcg ggcctgaggc caaggcagtg gtctgggcgc
     2521 acaactccca tatcggcaat gcagccttca ccgaaatggg ccaggtgcgc ggcgagcaca
     2581 atatcgggca gctggcgcgc cagcgtttcg gcgaggatgc ggcgctgatc ggtttcggca
     2641 ccgaccgcgg caccgttgcc gccgcttcgg actgggacgg ggacatggag atcaagcgcg
     2701 tgcgccctgc ccgcgacgat tcctatgagg gccggtcgcg cgacacgggc atcgccgcct
     2761 tcctgctcga gaccgggccg ggacaaagag agacggtgcg cagcgagctt gccgagccgc
     2821 tgctcgagcg cgccatcggc gtcatctacc ggcctgaaac cgagctgctc tcgcactact
     2881 ttcaggccga gctgtcgcgt cagttcgatg cctggatctg gtttgccgaa acccaagcgg
     2941 tggcggcacg cccagtgcac gcccacggcc ccgacgagac ctatccgttc ggtctttagt
     3001 gccgcgtccc aaactgcggc agcgattttg tttcattttg tttctcgtcc caaggattgc
     3061 ctttttgtaa cgcttagctc tccccggttc gtgctaatcg gctgggccag gggtaagcat
     3121 gaagacaggc tggttgctca ctctcgtggt ggccgctgcg gcaggcgctg gcgcctatta
     3181 ctactggcat cagcaagggc cgggccggct cgacattcag acggccgagg tcaagcgcgg
     3241 cgatgtgcgc cgcgtggtgt cgacctcagg cacggtgagc gcgctcgtca ccgtcgatat
     3301 cggctcgcag ctctcaggca atatcggcgc ggtcaacgtc gactattcga gcgaggtcaa
     3361 gcagggcgag gtgctcgccc gcatcgagcc gtcgagcttc gagacgcgcg tgcgcgagga
     3421 ggaagccgcg gtcgccgtgg ccaaggccaa tgtcgacctg caacaagccg gcatcgggcg
     3481 ggctgaggcc aatctgcaca aggcagagct cgacctcggc cgggccaagg agctcgtcgc
     3541 caaaggcgcc acgtcgcagg cggctctcga caccgccatc gccgccgagc aaggcgccat
     3601 cgccgacctc gccatcgcca aggccaatgt cgagaatggc aaggcgacgc tgacccagca
     3661 tgaggcgacg ctcgacagcg cccgtatcga cctcggccgc acctatatcc gctcgcccat
     3721 cgacggcgtg gtcatcgaca agatcgtcga ggtcgggcag accgtggcgg caagcttgca
     3781 ggcgccgaag ctcttcacca tcgcccagga tctcaaccag gtgcagatcc aggcgcaggt
     3841 cgatgaggcc gatatcggcc aggtgacgag cggcaacccg gtcagcttca ccgtcgacgc
     3901 ctatcctgac gtgaccttcg aggggaaggt cgagcagatc aggcttgcgc cgatctcctt
     3961 gcagaacgtc gtcacctata ccgtggtcat cgccgctgac aatcagctcg gccggcttct
     4021 gcccggcatg accgccaatg tcgagatcat caccggcgag catcctggcg tcgtggtggt
     4081 gcccaacgat gctctgcgct tccagccgcg cgggccggcc gaggcgttga tcagcgacgc
     4141 aacggcgacg gtctcaatac cgacagcgtc ggcagccggg gagcgcacca accggctgct
     4201 cgacaggctc aaggtcgacc tcgagctca
//
LOCUS       DATNAE010000231         2427 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2944915, whole genome shotgun sequence.
ACCESSION   DATNAE010000231 DATNAE010000000
VERSION     DATNAE010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2944915"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="VN518_05235"
     CDS             <1..609
                     /locus_tag="VN518_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HXE04010.1"
                     /translation="LKMDLAGAFQLGLLTMVLTLLLVMLLDTAGTLIGVARQAGLLDQ
                     DGRLPRLRQALLADSGGAMLGAALGTSTTTAYIESAAGVEEGGRTGLTALMVTVLFLA
                     SLFLAPLAQTVPPYATAPALLFVACLMATSLGAIQWDEPTEYIPALTIALMIPLSYSI
                     ATGIGLGFIAYVALKLLWGRVREINAASGTIAGAFLLKLIFA"
     gene            complement(630..812)
                     /locus_tag="VN518_05240"
     CDS             complement(630..812)
                     /locus_tag="VN518_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE04011.1"
                     /translation="MQISEVHDYASRLLREHGDKAQLIAAQRVLECEKRGDRSEAEDW
                     RRIRDTLKEMKGPLAS"
     gene            985..1278
                     /locus_tag="VN518_05245"
     CDS             985..1278
                     /locus_tag="VN518_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011082959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HXE04012.1"
                     /translation="MPKYILLANFTDQGIRNIRGTIGRAEAFQAMAEKSGIVMKELCW
                     TLGRYDVVAVFEAADDESAAALALSASSLGNTRCETLRAFSFDEMRRTLSKMV"
     gene            1363..1797
                     /locus_tag="VN518_05250"
     CDS             1363..1797
                     /locus_tag="VN518_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE04013.1"
                     /translation="MRRRMISLFAGLALISMTGLAAAGPFGKPDAPSLIVPVWSLTGE
                     CYPIDFNGIDIEWYAGRGVYQLTVTGTKPYMNMDVSLSHEAYNGRPAYWRTVVIGCVK
                     NGLLMPLASPYYITMNLDQFVGSRGVEIVGASRKVRRDIPKS"
     gene            1940..2278
                     /locus_tag="VN518_05255"
     CDS             1940..2278
                     /locus_tag="VN518_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE04014.1"
                     /translation="MDTEASPRPRHGGDHPIAIFITSISAAIVVAGGFGIIIIAFIVA
                     GADLFDLGQAFVLVGSVITGLITLWLFAWTFARSLHVERRLAAGLEIDEPKLSILANF
                     RDSGPVPSRS"
BASE COUNT          394 a          802 c          724 g          507 t
ORIGIN      
        1 ctcaagatgg acctcgccgg tgcctttcag cttgggcttc tcaccatggt gcttacgctt
       61 ctgctcgtca tgcttctcga cacggcggga acgctaatcg gcgtggcgcg acaggctggg
      121 ctgctcgatc aggacggacg cctgccgcgg ctccgccagg cgctgcttgc cgattccggc
      181 ggggccatgc tcggcgccgc gctcggcacc tcgacgacca ccgcctatat cgaaagcgcc
      241 gctggcgtag aggagggcgg ccgcaccggg ctcaccgcgc tcatggtgac ggtgctgttt
      301 ctggcgagcc tgttcctggc gccgctcgcc cagaccgtgc cgccttacgc cacggcacct
      361 gcgctcctct tcgtcgcctg tctcatggcg acgagcctcg gcgcgatcca atgggacgag
      421 ccaaccgaat atatcccggc gctcaccatc gcgctgatga tcccgttaag ctattcgatc
      481 gccaccggca ttgggctcgg cttcatcgcc tatgtggcgc tgaagctgct ctgggggcgc
      541 gtgcgcgaga tcaacgccgc ctcggggaca atcgccggcg ccttcctgct caagctgatc
      601 tttgcctgac ggcttgggtg agcggcggct cagctcgcca acggtccctt catctctttc
      661 agcgtgtcgc ggatgcggcg ccagtcctcg gcttcgcttc tatcgccgcg cttctcgcat
      721 tcaagcaccc tttgcgccgc gatcaattgg gccttgtcgc catgctccct aagcagcctg
      781 ctcgcgtaat cgtgcacctc tgagatctgc atgggctacc tccttcgtca tcgaagcaac
      841 atgagcaacg ggaaggcgct gactcgggtt cctctctgcc aggacccacc aatcacggcc
      901 cgacgccttg ccgcgcaagc tgattttaac gcgaccgctg cctcatagta gacttccgcg
      961 gcaggactgg agaggagaaa tccgatgccg aaatatattt tgctcgccaa cttcaccgat
     1021 cagggcatac gcaacatcag gggtacgatc ggccgcgctg aggcctttca agcgatggcc
     1081 gagaagagcg gcatcgtcat gaaagaactc tgctggaccc ttggccgcta cgatgtcgtc
     1141 gcggtgttcg aggcggcgga tgatgagagc gcggctgcgc tcgcgctcag cgcctcctcg
     1201 ctcggcaata cgcggtgcga aaccctgcgt gccttttcct tcgatgagat gaggcgcaca
     1261 ctgagcaaga tggtttgagg ccggcccttt gcgtcttcgc gctgaccttg aggcggtcac
     1321 agtcgcccgt taggctatcg tccttggact tggggggatg cgatgcgacg ccgcatgata
     1381 agcctgtttg ccgggttggc gctgatttcg atgaccggac ttgccgcagc cggaccgttc
     1441 ggcaagcctg atgcgccgtc cctcattgtg ccggtctgga gcctcaccgg cgagtgctat
     1501 cccatcgatt tcaacggcat cgatatcgaa tggtatgccg gccgcggcgt ctatcagctc
     1561 accgtcaccg gcacgaagcc ctacatgaac atggacgtct cgctgagcca cgaagcctat
     1621 aacggccgcc cggcctattg gcgcacggtc gtcatcggtt gcgtcaagaa cggcctgctg
     1681 atgccgctcg cctcgcctta ttacatcacc atgaaccttg accagttcgt tggctcgagg
     1741 ggggtcgaga tcgtcggcgc ctcgcgcaag gtccgccgcg acattcccaa gtcctaagga
     1801 ttccggtccc atccttcgag acgcgctctt cgagcgctac tcaggatgag gtccctcatg
     1861 gtgatgagcg gccaaaggcc gcgtctcgta ccatgaggcc tcttgtgctt tccacggcgt
     1921 ctccgctatt aggtcgccca tggacactga agccagccct cgtccccgcc acggcggaga
     1981 ccaccccatc gccatcttca tcacctcgat cagcgccgcg atcgtggtcg ccggcggctt
     2041 cggcatcatc atcatcgcct tcattgtcgc cggcgcagac ctgttcgacc tcggccaggc
     2101 ctttgtgctg gtaggcagcg tcatcaccgg gctgatcacc ctttggctgt tcgcctggac
     2161 gttcgcccgc tccctccatg tcgagaggcg gcttgccgcg gggcttgaga tcgacgagcc
     2221 aaagctctcg atcctggcca atttccgtga ttccggaccg gtcccgtcga ggtcttgagc
     2281 gagccagaaa cgaccgcccg cgagacgatt atccgcatgc ggtacgccgt aaaaatcctt
     2341 atgctctagc cggtatttgt tgggggggtc ggcaatgtcg atttggggaa agttggccgg
     2401 cgccgcggct gggctcgcaa tcggcgg
//
LOCUS       DATNAE010000232         2541 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3535540, whole genome shotgun sequence.
ACCESSION   DATNAE010000232 DATNAE010000000
VERSION     DATNAE010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3535540"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="VN518_05260"
     CDS             <1..517
                     /locus_tag="VN518_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014132039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HXE04015.1"
                     /translation="KELERLERAGQRVPERLELGAMIEVPSLLFELDQLLPEADFVSI
                     GSNDLVQFLTAADRANPRVAKSYDPIAMPRLRVFRHVVDMAKRFNVPLTMCGELAGKP
                     MEALALMAIGMNRLSMGPPSIGPIKEMILGLDLEPIRRSVAAALLEGSDGMPIRELLT
                     EWVERQNLPIA"
     gene            734..1810
                     /gene="prfA"
                     /locus_tag="VN518_05265"
     CDS             734..1810
                     /gene="prfA"
                     /locus_tag="VN518_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016736489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HXE04016.1"
                     /translation="MPSLPQDKLDALERRFSSIEAELAAGAAGDAFVRLSKEYAELEP
                     IVRPILAYQKLLADLRAAEELAASPDREMAEMAEAEIAEIREKLDQMEHAIRILLLPR
                     DEADEKNAILEIRAGTGGDEAALFAGDLLRMYQRYSDLQGWKFSILEESAGDAGGYKE
                     VIASVHGRNVFARLKFESGVHRVQRVPATEASGRIHTSAATVAVLPEVEDIDIEIRPE
                     DIRIDTMRSSGAGGQHVNTTDSAVRITHIPTGIVVTSSLKSQHQNRAQAMIVLRARLY
                     EEQRNARDTARSQSRREQVGSGDRSERIRTYNFPQGRVTDHRINLTLYKLDKVVAGDA
                     LDELIEALIAENQAALLAEMEGIS"
     gene            1807..>2541
                     /gene="prmC"
                     /locus_tag="VN518_05270"
     CDS             1807..>2541
                     /gene="prmC"
                     /locus_tag="VN518_05270"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HXE04017.1"
                     /translation="MTTLGEAFRVVRDKFRAAELDTPELDAKLLAEAAFGLDKLSLSL
                     REKEDAPADGLSRLDSLAARRLDGEPVARLLGHKEFYGLDFALNAATLVPRPETELVV
                     DQGLAALKGKDAPVLLDLGTGSGCIAIALLVHAPQAHAVATELSPEAIAVARRNAERL
                     GVADRLDLRQGSWCEPLAADERFDLVVANPPYVETEIIPQLPAEVSGYDPVLALDGGE
                     DGLSAYRAIAEGMKGHLKPGGTLIVEI"
BASE COUNT          444 a          839 c          824 g          434 t
ORIGIN      
        1 caaggagctc gagcggctcg agcgcgccgg ccagcgcgtg cccgagcgtt tggagctggg
       61 cgcgatgatc gaggtgccgt cgctgctctt cgagctcgac cagctcctgc cggaagccga
      121 tttcgtttcg atcggctcca acgatctggt gcagttcctg acggcggcgg atcgcgccaa
      181 tccccgcgtc gccaaaagct acgatccgat tgccatgccg cgccttcgcg tcttccgcca
      241 cgtcgtcgac atggccaagc gtttcaacgt gcccctgacc atgtgtggcg agcttgccgg
      301 caagccaatg gaggcactgg cgctgatggc catcggcatg aaccggcttt ccatggggcc
      361 accttcgatc ggtcccatca aggagatgat cctcgggctc gacctcgagc cgatccgccg
      421 tagtgttgcc gccgccctgc tcgagggctc tgatggcatg ccgatccgcg aactgttgac
      481 cgagtgggtc gagcgccaga acctgccgat cgcatgagac cttgcatatt cgcgccgatt
      541 tcgccatttg cacgtcgtca atcccacgaa agcgggaatc cgcatggccg gacctcgccc
      601 cccttcgata ggctcgggat gagatccgcc cagagggcga gctcgtgatg agggccttta
      661 cggaggcctc acggtgagcc tttcgaacca tgcggccggc cccgagcgcg ggcagcccca
      721 acccggacac tgaatgccaa gtcttccaca ggacaagctc gacgcgctcg agcgtcgttt
      781 ctcttcgatc gaggccgagc tcgccgccgg tgcggcaggc gatgcgttcg tgcgcctctc
      841 caaggaatat gcggaactcg agccgatcgt ccggccgatc ctcgcctatc agaagctcct
      901 cgctgatctc cgcgcggctg aggaactggc ggcctcgcca gaccgcgaaa tggccgagat
      961 ggctgaagcc gagatcgccg agatcaggga aaagctcgac cagatggagc atgcgatccg
     1021 catcctgctg ctcccccgcg acgaggcgga cgagaagaac gcaatcctcg aaatccgggc
     1081 cggcaccggc ggcgacgagg cggcgttgtt cgctggcgac ctcctgcgca tgtatcagcg
     1141 ctattccgat cttcagggct ggaagttctc catcctcgaa gaaagcgccg gcgacgcggg
     1201 cggctataag gaagtgatcg cctcagtcca cgggcgcaac gtcttcgccc ggttgaaatt
     1261 cgagagcggc gtgcatcgcg tgcagcgtgt gccggcaacg gaagcgtccg ggcgcatcca
     1321 tacctcggcg gcgacagttg cggtgctgcc cgaggtcgag gatatcgaca tcgagatccg
     1381 cccggaggat atccgcatcg ataccatgcg ctcgtcgggc gccggagggc agcacgtcaa
     1441 caccaccgat tcggcggttc gcatcaccca tatcccgacc gggatcgtgg tgacctcgag
     1501 cctcaagagt cagcaccaga accgcgccca ggcgatgatc gtgctcaggg ccaggctcta
     1561 cgaagagcag cgcaacgccc gggacaccgc ccgttcacag tcgcgccgcg agcaggtggg
     1621 ctccggcgac cgttccgaac ggattcgcac ctataatttt ccgcagggcc gcgtcaccga
     1681 ccaccgcatc aatctgacgc tctacaagct cgacaaggtc gtcgccggcg atgccctgga
     1741 cgaactgatc gaggcgctga tcgccgagaa ccaggcggcg ctgctggcgg agatggaggg
     1801 catctcctga ccaccctcgg tgaagccttt cgggttgtgc gggacaagtt ccgcgccgcc
     1861 gagctcgata cacccgagct cgatgcaaag cttttggcgg aagcggcttt cggtctcgac
     1921 aagctgtcgt tgtcgctgcg cgagaaggag gacgcgcccg ccgacggcct gtcgcggctc
     1981 gattcgctgg cggcgcggcg gctcgatggg gagccggtgg cgcgtctgct cggccacaag
     2041 gaattctacg gccttgattt tgcgttgaac gcggcgacgc tggtgccgcg gccggaaacc
     2101 gagctggtcg tcgaccaggg actcgccgcg ctcaagggaa aggacgctcc ggttctcctc
     2161 gatctcggca ccggcagcgg ctgcatagcc attgcgctgc ttgtccacgc cccccaggcc
     2221 cacgccgtcg ccacggagct ttcgccggag gccattgccg tcgcgcggcg gaacgccgag
     2281 cgactcgggg tcgctgatcg gctggatctg cggcagggat catggtgcga gccgcttgcc
     2341 gcggacgagc gcttcgatct cgtcgtcgcc aatccgccct atgtcgagac cgagatcatt
     2401 ccgcaattgc ccgccgaggt ctccggctac gacccggtgc tggcgctcga cggcggcgag
     2461 gatgggcttt cagcctatcg ggcgatcgct gaagggatga agggccatct caagccgggc
     2521 gggacactga tcgtcgagat c
//
LOCUS       DATNAE010000233         5441 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3240085, whole genome shotgun sequence.
ACCESSION   DATNAE010000233 DATNAE010000000
VERSION     DATNAE010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5441
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3240085"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1064)
                     /locus_tag="VN518_05275"
     CDS             complement(<1..1064)
                     /locus_tag="VN518_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HXE04018.1"
                     /translation="MRKSEYALWALLVLAVLAAGSTMVSLARSQSASAANSEIYRQLD
                     LFGEVLERVRSDYVEKPDDPQLIESAINGMLTALDPHSAYLNPKHFRDMQVQTRGEFG
                     GLGIEVTMENGVIKVVAPIDDTPAAHAGLMSGDLITHLDKEQVLGLTLQEAVDKMRGP
                     VNSPITLTVVRKGTEDPFDVKVTRDVIHINPVKYNAEGNDVGYIRVTTFNEQTTANLQ
                     KAIEDLKKQLGPNLKGFVIDLRNNPGGLLDQAISVSDAFLDQGAIVLTRGRDLEETQR
                     SNARPGDITDGQKIVVLINGGSASASEIVAGALQDHHRATILGTRSFGKGSVQTIIPL
                     GSNGALRLTTARYYTPSGRS"
     gene            complement(1064..2422)
                     /locus_tag="VN518_05280"
     CDS             complement(1064..2422)
                     /locus_tag="VN518_05280"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HXE04019.1"
                     /translation="MAHPDRLTSPRHRFWSALLVAGLVCASPWLAAAEEDLSRDEAKQ
                     KLNQTEQELQSSRAKAEGLSKDLAALAEERAKLNNELIDAGKSVQASEAKLSETETKL
                     AEFTEQVNVIQNSITERKEAIVKMLSAMQRIGRTPPPALVTRRDDALAVVRSAMLLAN
                     IFPELKYQADNLSHELEGLVTVENGIRDQRDKEKSEAERLAAEQASLDRLLEEKKRAA
                     AQGEAELAALKQSTAEQAQTVTDLNELIDRLDTQIAKVEIAQYDAELAAERALLAREQ
                     QQALATPANESVVEIKPGTKVAFASPDRLKPALPFEAAKGSLRLPAQGRTVKRFGDPD
                     AAGGTLKGISLQTRGEARITAPADGWVVYAGEFRSYGQLLIINAGGGYHILLAGMNRI
                     DVSLGQFVLAGEPIAVMGNSAAASQGEDSSRPVLYVEFRKDGRPVDPDPWWAQASEKV
                     QG"
     gene            complement(2512..2994)
                     /gene="rlmH"
                     /locus_tag="VN518_05285"
     CDS             complement(2512..2994)
                     /gene="rlmH"
                     /locus_tag="VN518_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011155735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HXE04020.1"
                     /translation="MRLLIAAIGKLKQGPERDLFAHYLGRAEAAGRKLHLTPLDSIEI
                     AEAKGSTQQARMEAEAEALLAKRPPSYKLICLDPGGTELASEAFAGILAKFRDGGAPG
                     TAFFVGGADGLGLTALFKADLALSLGKMTLPHGLARIVLAEQIYRAATILAGHPYHRA
                     "
     gene            complement(3032..3502)
                     /gene="rsfS"
                     /locus_tag="VN518_05290"
     CDS             complement(3032..3502)
                     /gene="rsfS"
                     /locus_tag="VN518_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HXE04021.1"
                     /translation="MRIGYSGFVEEQWQKDTHPNMRSAREGAAHGTPPNGFASFGAHP
                     NRLLKIVLDTLDAAKAEKVATIDLRGKSSIGDQMVVASGRSQRHVGAVADQIIKKLKD
                     EGFGRARVEGTPQCDWVLIDAGDVIVHIFRPEVREFYNLEKMWSADRPMERLVI"
     gene            complement(3581..4234)
                     /locus_tag="VN518_05295"
     CDS             complement(3581..4234)
                     /locus_tag="VN518_05295"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HXE04022.1"
                     /translation="MAAPNMRIGLLGGSFNPPHDAHRAISLTALKRLGLDQVWWLVTP
                     GNPLKDASELPSLKSRVEAAQDLARHPRIAVTGFEAGSGSAYTVDLLAELKRRFPGVR
                     FVWLMGADNLAQFDRWRAWEEIFAAVPIAVLDRPGFRLKARAGKAAQRFAAYQVDESD
                     ARGLARLEPPAWTILSHTLSGLSSTELRAKQAKTPKKAKRDKAKDKVKAPKKKAKRH"
     gene            complement(4351..>5441)
                     /locus_tag="VN518_05300"
     CDS             complement(4351..>5441)
                     /locus_tag="VN518_05300"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013417933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HXE04023.1"
                     /translation="AQNKGRDAAFLDRLRLDGMRVAAMARGLREVAALADPVGQVMAH
                     WTRPNGLEISRVRVPLGVIGIVYESRPNVTADAAGLCLKSGNAVILRSGSESFLTSLV
                     IANALSEALLASGLPEASIQLVPTVDRAAVGFMLGGLAGAVDVIVPRGGKDLVARVQD
                     EARVPVFAHLEGVCHVYVHKAADLDMAKHIVLNAKLRRTGVCGATETLLVDRAVATTH
                     LAPLVEMLIEAGCEVRGDPQTQRADSRVKVASEEDWPKEFLDAIIAVKVVDGLDEAIE
                     HIARYGSQHTDAIVTEDERAAERFLACVDSAIVLHNASTQFADGGEFGMGAEIGIATG
                     RFHARGPVGLEQLTTFKYVVRGQGQIRP"
BASE COUNT          888 a         1791 c         1720 g         1042 t
ORIGIN      
        1 atcgagcggc ccgacggcgt gtaatagcgc gcggtggtaa gcctcagcgc accgttcgag
       61 ccgagtggaa tgatggtttg caccgatccc ttaccgaagg aacgcgtgcc gaggatggtc
      121 gcgcggtgat gatcctgcaa cgcgcccgcc acgatctccg atgccgaggc cgagccgcca
      181 ttgatcaaaa cgacgatctt ctgtccgtcg gtgatgtcgc ccggccgcgc attggagcgc
      241 tgcgtctcct cgagatcgcg gccgcgcgtc agcacgatgg cgccctggtc gaggaaggca
      301 tccgagacgg agattgcctg atcgagcagg ccgcccggat tgttgcggag gtcgatgaca
      361 aagcccttga gattcgggcc gagctgcttc ttcaggtcct cgatcgcctt ctgcagattg
      421 gcggtggtct gctcgttgaa ggtggtgacg cggatatagc cgacgtcgtt gccctcggcg
      481 ttgtacttca ccggattgat gtggatcacg tcacgcgtga ctttgacgtc gaacggatcc
      541 tcggtgccct tgcgcacgac ggtaagcgtg atcggcgaat tcaccggtcc gcgcatcttg
      601 tccaccgcct cttgcaaagt gaggccgaga acctgctctt tgtcgagatg ggtgatgaga
      661 tcgccgctca tcaggccggc atgcgcggcg ggggtatcat cgatcggggc gaccaccttg
      721 atgacgccat tctccatcgt gacctcgatg ccgaggccgc caaattcgcc ccgcgtctgc
      781 acctgcatgt cgcggaaatg tttcggattg agataggccg aatgcggatc gagcgcggtc
      841 agcatgccgt tgatggccga ctcgatcagc tggggatcgt cgggcttctc gacatagtcc
      901 gagcgcacgc gctccaggac ctcgccgaac aggtcgagct ggcggtaaat ctccgagttg
      961 gcggccgacg ccgactggct acgcgccaag ctcaccatcg tcgatccggc agcgagcacg
     1021 gccaacacca gaagcgccca cagcgcgtat tcggatttcc gcatcatcct tgcacctttt
     1081 cggaagcctg ggcccaccat gggtcagggt ctactggccg gccgtccttt ctgaattcga
     1141 catagaggac ggggcgcgaa ctgtcttcgc cttggctcgc cgctgccgag ttgcccatca
     1201 ccgcgatcgg ctctccggca aggacaaact ggcccaggct tacgtctatg cggttcatcc
     1261 ccgcaagcag gatatgatag cccccgcctg cattgatgat caatagctgc ccataggagc
     1321 ggaactcccc ggcatagacc acccagccgt ccgccggggc ggtaatccgg gcttcgccac
     1381 gggtttgcag cgagattccc ttgagcgtgc cgcccgcggc atcggggtcg ccgaaccgct
     1441 tcacggtccg cccctgagcg ggcagccgca ggctgccttt cgccgcctcg aagggaagtg
     1501 ccggcttgag ccggtcgggg ctggcaaagg cgactttggt ccccggcttg atctcgacca
     1561 cgctctcatt ggccggcgtg gccaaggcct gttgctgctc ccgcgccagc aaagcgcgct
     1621 cggcggcgag ctcggcgtcg tattgagcga tctcgacctt ggcgatctgc gtatcgagcc
     1681 ggtcgatcag ctcgttcaaa tccgtgacgg tctgcgcctg ctcggccgtg gactgcttga
     1741 gcgcggcgag ctcggcctcg ccttgcgccg cggcgcgctt cttctcctcg agcaaccggt
     1801 cgaggctcgc ctgctccgcc gccaaccgct cggcctcgct cttctccttg tcgcgctggt
     1861 cgcgaatgcc gttctcgacc gtgaccaagc cttccagctc atgcgagagg ttgtccgcct
     1921 gatatttcaa ttcggggaaa atattggcga gcagcatggc gctgcgcacg acggcaagcg
     1981 catcgtctcg cctggtgacc agcgccggcg gcggtgtgcg gccgatcctc tgcatggcgc
     2041 tcagcatctt gacgatggct tccttgcgtt cggtgatcga attttggatg acgttgacct
     2101 gctcggtgaa ctcggcgagc ttcgtttccg tctccgagag cttcgcttcg ctcgcctgca
     2161 cgctcttgcc ggcgtcgatc agctcgttgt tcagtttcgc ccgttcctcg gccagcgccg
     2221 ccagatcctt gctcaaccct tctgccttgg cccggctcga ctgaagctcc tgctcggtct
     2281 ggttgagctt ctgcttcgcc tcgtcgcggg agagatcctc ctcggccgcg gcaagccacg
     2341 gcgacgcaca gaccaagccg gcgacaagca gcgcggacca gaacctgtgg cggggagaag
     2401 tcaatcgatc aggatgcgcc atgcgccttg gccgttcagc cgatggcaac gacaacggag
     2461 cggtacggca gaatcaaggc aattggctta ccaatcctta accttgggcc atcaggcccg
     2521 gtgatagggg tggcccgcca ggatggtcgc cgcgcgatag atctgctcgg cgagaacgat
     2581 gcgggcgagc ccatgcggca aggtcatctt gccgagcgaa agcgcgaggt cggccttgaa
     2641 taacgccgtc agccccaggc cgtcggcgcc gccgacgaag aacgccgttc caggcgcgcc
     2701 gccgtcgcgg aacttcgcca agatgccggc aaaggcttcg ctggcgagct cggtcccccc
     2761 tgggtcgagg cagatcaatt tatatgaggg tggacgcttg gcgagcagcg cctcggcctc
     2821 ggcttccatc cgcgcctgct gcgtcgagcc tttggcttcg gcgatttcga tgctgtcgag
     2881 cggtgtcagg tggagcttgc ggcccgccgc ttcggcgcgg ccgagataat gcgcgaacag
     2941 atcgcgctcc ggcccctgtt tcagcttgcc gatggcagcg atcaggagcc gcatgcttgc
     3001 tcgctttagg ctaagcggcc gggcaatcgg atcagatcac caaccgctcc atggggcgat
     3061 cggccgacca catcttttcg aggttataga actcgcgaac ctcgggccga aagatatgga
     3121 cgatgacgtc gccggcatcg atcagcaccc aatcgcattg gggcgtccct tcgacgcgag
     3181 cgcggccaaa gccttcgtct ttcagctttt tgatgatctg gtcggcgacg gccccgacat
     3241 gccgttgcga gcgtcccgat gcgaccacca tctggtcgcc gatcgagctt ttgcctctca
     3301 agtcgatggt cgcgaccttc tccgccttgg cggcatcgag cgtgtcgagc acgatcttga
     3361 gtagacggtt cggatgcgcg ccaaatgacg cgaatccgtt gggaggtgtg ccgtgcgcgg
     3421 caccctctct agctgagcgc atgtttgggt gagtatcctt ctgccattgc tcctcgacga
     3481 agccgctata cccgatacgc atgctaaacg catgacaagg cgtttcggat gccgcttcgc
     3541 accataaaca tagtatgacc gccgcctgcg ctcaatagct ctagtgtcgc ttggcctttt
     3601 tcttgggcgc cttcaccttg tccttcgcct tgtcccgctt cgctttcttc ggcgttttag
     3661 cctgcttggc gcgaagttcg gtcgaggaga gcccggagag cgtatggctg aggatggtcc
     3721 aggcgggagg ctcgagccgc gccagcccgc gtgcatctga ttcgtcgacc tggtaggcgg
     3781 cgaatcgctg tgccgccttg ccggcgcgcg ctttcagcct gaacccaggg cgatccagca
     3841 ccgcgatcgg cacggcggcg aaaatctcct cccaggcgcg ccagcgatcg aattgcgcga
     3901 gattgtcggc gcccatcagc cagacgaagc gcacccccgg aaagcgccgc ttgagctcgg
     3961 cgagcaaatc gaccgtgtag gcagagccgc ttcccgcctc gaagccggtc acggcgatgc
     4021 gcggatgcct ggccaagtct tgcgcggcct cgacgcggga cttgaggctc ggcagctcgg
     4081 aggcatcttt cagaggattg cccggcgtga ccagccacca aacctgatcg agcccgagcc
     4141 gtttcagcgc cgtgagactg atggcgcggt gcgcgtcgtg tggcgggttg aacgagccgc
     4201 cgagcaggcc gatgcgcata ttgggcgccg ccatcggcag cttcgtgaca tccagccggg
     4261 gggtcttcga ctcatgtttg ccatcgccct tgcgatgggt gtgcttcttc tcggcgtgct
     4321 tttccttcga cacggcgtcc ctaagcggtt ttaaggcctg atctggcctt gcccgcgcac
     4381 gacatatttg aacgtcgtga gctgctcgag acccaccggg ccgcgcgcat ggaaccggcc
     4441 ggtggcgata ccgatctcgg cgcccatgcc gaactcgccg ccatcggcga attgcgtcga
     4501 ggcgttatgc agcacgatgg cgctgtcgac gcaggcgagg aaccgctcgg cggctcgttc
     4561 gtcttcggtg acgatggcgt cggtgtgttg cgagccgtag cgcgcgatgt gctcgatcgc
     4621 ctcgtcgagg ccgtcgacca ccttcacggc gatgatggcg tcgagaaact ccttcggcca
     4681 atcctcctcg ctcgcgacct tcacccggct gtcggcgcgt tgcgtctgcg gatcgccccg
     4741 tacctcgcag cccgcctcga tcagcatttc gaccagcggc gccagatgcg tggtcgcgac
     4801 agcacgatcg accagcagcg tctcggttgc cccgcacacg ccggtgcggc gcagcttggc
     4861 gttgagcacg atatgcttcg ccatgtcgag atcggcagcc ttgtgcacat agacatggca
     4921 gacgccttcg agatgcgcga ataccggcac gcgcgcttcg tcctgcacgc gcgccacgag
     4981 atccttgccg ccgcgcggca caatcacgtc gacggcgccg gcaaggccgc cgagcatgaa
     5041 tccgaccgcc gcgcgatcga ccgtcggcac cagctggatc gaggcctcgg gcagacccga
     5101 ggcgagcagg gcctcgctca acgcattggc gatgacgagc gaggtgagaa agctctcgga
     5161 gccgctgcgc aggatcacgg cgttgcccga cttcaggcaa agccccgccg cgtcggcggt
     5221 cacattcggc cgcgactcgt agacgatgcc gatcaccccc agcggcacgc gcacgcggga
     5281 aatctccagg ccgttcggcc gcgtccagtg cgccatcacc tggcccaccg gatcggcaag
     5341 cgccgccacc tcgcgcaagc cgcgcgccat cgccgccacc cgcattccgt ccaacctgag
     5401 ccggtcgagg aaggcggcgt cacgaccctt gttctgggcg g
//
LOCUS       DATNAE010000234         2019 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2639907, whole genome shotgun sequence.
ACCESSION   DATNAE010000234 DATNAE010000000
VERSION     DATNAE010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2639907"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1263
                     /gene="odhB"
                     /locus_tag="VN518_05305"
     CDS             <1..1263
                     /gene="odhB"
                     /locus_tag="VN518_05305"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HXE04024.1"
                     /translation="ADEPLVELETDKVTVEVPAPASGVLSEIVVKAGDTVAVGSVLGA
                     IAEGDGKVKPAPVKAAAAAPQPVPAPVKPPEPPKPAKMQPAALEREPSPREEVSDSGN
                     GNGAGTATAEMPPSPAARKYLAETGLSPASVVGSGRRGQVLKQDVLAQAGAAPSGAVA
                     AAAASLLAREPKPKAPPAPRPPITPQDETREERVRMTRLRETIAKRLKEAQDTAAMLT
                     TFNDVDMSAVMQLRREYREAFEKKHGVKLGLIGLFVKACVQALKDIPAVNAEIDGTDI
                     IYKNYYHIGVAVGTERGLVVPVLRDADRMSVAAIDTAIADFGKRALQGKLGLEEMQGG
                     TFTISNGGVYGSLLSTPILNAPQSGILGMHRIEERAVVRDGQIVARPMMYLALSYDHR
                     LVDGKEAVTFLVQVKEAIEDPKRLVLDL"
     gene            1330..>2019
                     /locus_tag="VN518_05310"
     CDS             1330..>2019
                     /locus_tag="VN518_05310"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HXE04025.1"
                     /translation="MRPLKMLLRLCVLLGVAFLARAEGPPPEPRTALIIGNSAYSFAP
                     LKNPINDAEAMASALEGAGFKVIKETDADQAKMVEAVRTFGDELKRRGGVGLFYFSGH
                     GTQIDGENYLLPVGEQFASLEDIKRRSVTALAVVEAMASAHSDLNIFILDACRNNPID
                     PNGAKGLSRIDSNARLFISYATSPGLTALDGEGNNSPYAKYLYQSIDAPNLDIEDTFK
                     RTLKGVYVETHG"
BASE COUNT          394 a          634 c          647 g          344 t
ORIGIN      
        1 gccgatgagc ctctggtcga gcttgaaacc gacaaggtga cggtcgaagt gccggcgccc
       61 gcctcgggcg tgctttcgga gattgtcgtc aaggccggcg ataccgttgc cgtgggatcg
      121 gtgctcggcg ccattgccga gggtgacggc aaggtgaagc ccgcgccggt caaggccgca
      181 gcggcggcac cgcaacctgt cccggctccg gtgaagccgc cagagccacc caagccagca
      241 aagatgcaac cagccgcgct cgaacgcgag ccctcgccgc gggaagaggt gagcgacagc
      301 ggcaacggga acggcgccgg tacggccaca gccgaaatgc cgccgtcgcc cgccgcgcgc
      361 aaatatctcg ccgagaccgg gctttcgccg gcaagcgtcg tgggctcagg ccgccgcggt
      421 caggtcctga agcaagatgt gctggcgcaa gccggtgccg cgccgtcggg agcggttgcc
      481 gctgcggcag cttcgctgct cgcccgcgag ccgaaaccca aggcgccgcc ggcgccccgt
      541 ccgccgataa ctccgcagga cgagactcgc gaggagcggg tgcgcatgac gcggctgcgt
      601 gagaccatcg ccaagcggct gaaggaggca caagacaccg ccgccatgct caccacgttc
      661 aacgacgtgg atatgagcgc ggtgatgcag cttaggcgcg aataccgcga ggccttcgag
      721 aagaagcacg gggtgaagct cgggctgata ggattgttcg tgaaggcctg cgtgcaggcg
      781 ctgaaggata tccccgcggt caatgccgag atcgacggca ccgacatcat ctacaagaac
      841 tattaccata tcggcgtcgc cgtcggcacc gagcgcgggc tcgtggtgcc ggtgttacgc
      901 gatgccgacc gcatgagcgt tgccgcgatc gacacggcaa tcgccgattt cggcaagcgg
      961 gcgctccaag ggaaattggg actagaggag atgcaaggcg gcaccttcac catctccaac
     1021 ggcggcgtct acggctcgtt gctctccacc ccgatcctga acgcgccgca atcgggcatt
     1081 ctcggcatgc atcgcatcga ggagcgcgcc gtggtgcgcg acgggcagat cgtggcgcgg
     1141 ccgatgatgt atctcgcgct ctcctacgac catcgcctgg tcgatggtaa agaggcagtc
     1201 accttcctgg tgcaggtcaa ggaagcgatc gaggatccaa aacggctcgt tctcgatctt
     1261 tagcgacagc gctggaaggc tggcccggtc actggtaagt gtttgctgga ggcattctgg
     1321 gagccagtga tgcgtccgct taagatgttg cttcgcttat gtgttttgct cggcgtcgct
     1381 ttccttgcgc gtgccgaagg cccaccgcca gagccgcgca cggctttgat tatcggcaat
     1441 tccgcctatt ccttcgcgcc gctcaagaac ccgatcaatg acgcagaggc catggcatcc
     1501 gcgcttgaag gcgctggctt caaggtcatc aaggagaccg atgccgatca ggccaagatg
     1561 gttgaggccg tccgcacctt cggcgacgag ctgaagagac gcggcggcgt cggcctattc
     1621 tatttctcgg gccacggcac gcagatcgac ggcgagaact accttctgcc ggtcggcgaa
     1681 cagtttgcga gcctggagga catcaagaga cgctcggtca cggcgcttgc cgtggttgaa
     1741 gccatggcct cggcgcatag cgatctcaac atctttatcc tcgatgcctg ccggaacaac
     1801 ccgatcgacc cgaacggtgc taaggggctg tcgcgcatcg attccaatgc gcgcctgttc
     1861 atctcctatg ccacctcgcc aggactgacc gcgcttgacg gcgagggcaa caacagtccc
     1921 tacgccaaat atctctatca atcgatcgac gcgccaaatc tcgatatcga ggacaccttc
     1981 aagagaacct tgaagggcgt ttatgtggag acccacggc
//
LOCUS       DATNAE010000235         3538 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_838190, whole genome shotgun sequence.
ACCESSION   DATNAE010000235 DATNAE010000000
VERSION     DATNAE010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838190"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="VN518_05315"
     CDS             complement(<1..232)
                     /locus_tag="VN518_05315"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HXE04026.1"
                     /translation="MSEALRRVAFGEIDVAQTALASPPDRHGGVHDARKCLKRLRSLL
                     LLASPGMPEPVFTSLTERVAAIARGLAPARDAH"
     gene            complement(346..978)
                     /locus_tag="VN518_05320"
     CDS             complement(346..978)
                     /locus_tag="VN518_05320"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012489802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HXE04027.1"
                     /translation="MLSLDPFYPILPDTEWLARLLPHGIKLVQLRIKDKSEAAVKDEI
                     ARAIALCRAHGCQLAINDYWREAIEQGARFVHLGQEDLANADLSAIRAAGIGLGVSTH
                     NVEELHAALAAKPDYVALGPIYATTLKAMPWAPQGLPRLAEWRAKIDCALVAIGGMTA
                     NRAPLALAAGADSVAVITDVVTSDNPEEQVRRWIAVTAPWRHLCTGTPQG"
     gene            complement(1004..1792)
                     /locus_tag="VN518_05325"
     CDS             complement(1004..1792)
                     /locus_tag="VN518_05325"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HXE04028.1"
                     /translation="MLTLYGATFTSRLLLGTAQYPSPECLKQAVEASGAEIVTVSLRR
                     ESARARTGQGFWALIEQLGAQVLPNTAGCRTAKEAIATANMARELFGTSWIKLEVIAN
                     DDTLQPDLFGLVEAAAELTKEGFEVLPYTTEDLSAAERLVALGCKVLMPWAAPIGSAR
                     GLINKDALKTLRAYFPDTPLIIDAGIGAPSHAAESMEMGYDAVLLNTAVAKAGDPVKM
                     AQAFARAIEAGRLAYEAGLIEPRDMASPSTPVAGTPFFKIDNAS"
     gene            complement(1792..1989)
                     /gene="thiS"
                     /locus_tag="VN518_05330"
     CDS             complement(1792..1989)
                     /gene="thiS"
                     /locus_tag="VN518_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HXE04029.1"
                     /translation="MQILLNGKRFATEARNLDELCAGLGFADAKIATAVNGSFVAASE
                     RKRTPLIEAAEIEIVAPRQGG"
     gene            complement(1967..2977)
                     /locus_tag="VN518_05335"
     CDS             complement(1967..2977)
                     /locus_tag="VN518_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXE04030.1"
                     /translation="MAARSQSIAIRGAGVVGLWQALLLARAGHAVTIYERSTTPFAEA
                     CSPFAGAMLAPRCEEESAEAVIGELGLRSIALWRATYPGIVVNGSLVVAPPRDQTLLD
                     RFARMTHGSERLTGDALAALEPDLAKRYATALYYPGEAHLAPDAALDFLLAEARAAGA
                     RLILGEGEMPKSADVIIDCRGLAARDKLPTLRGVRGERIVVRARDVALSRPVRLLHPR
                     FPLYAVPWGQSLFMIGATVVESEETGPVSVRSALELLSAAYALDPAFGEAEIVKLGAG
                     ARPAFPDNRPRIITAKGYIYVNGLYRHGFLLAPALAELVATYLSTGETHPEVFVADPA
                     QR"
     gene            complement(2977..>3538)
                     /locus_tag="VN518_05340"
     CDS             complement(2977..>3538)
                     /locus_tag="VN518_05340"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HXE04031.1"
                     /translation="AHGTSQVSIVDDDGNAFSMTTTIERSFGSRNMVRGFLLNNQLTD
                     FSFVPVDNAGSPVANRVEGGKRPRSSLDPTLVFANGDGALHYVLGSPGGAAIILYNIK
                     ALIALIDWRLDAADAAALVNFGSTGDAVVIEPGAEWNSLANALESLGHNVQRTDLVSG
                     LHIIAVTKDGLEGGADPRREGVALGD"
BASE COUNT          619 a         1098 c         1222 g          599 t
ORIGIN      
        1 catgcgcatc gcgcgcagga gccaacccac gagcgattgc ggcgaccctc tcagtgaggc
       61 tggtaaagac cggctcgggc attccggggc tcgcgagcag cagcaaagac cgcagccgct
      121 tcaagcactt acgtgcgtcg tgcacgccgc cgtgacggtc gggagggctc gcgagcgcgg
      181 tttgagcgac gtcgatctcg ccaaaggcga cgcgacgcag ggcttcgctc atgggtatag
      241 ccggatcgag acgataactc atcggagttt ccccgttgct cagctcccga attgtgccga
      301 atgatcccgg gggcggcggc ctgggtcaag caggaagcac cggcgtcagc cctgtggagt
      361 tcctgtgcac aagtggcgcc aaggcgcggt gaccgcgatc cagcgccgca cttgctcttc
      421 ggggttgtcg ctcgtcacga cgtcggtaat gacggcgacg ctgtcagcgc ccgcggcgag
      481 agcgagcgga gcgcgattgg ccgtcatgcc gccaatggcg acgagcgcgc agtcgatctt
      541 ggcacgccat tcagcaagac ggggcaggcc ttgcggcgcc cacggcatcg ccttgagcgt
      601 cgtcgcgtag atcggtccga gagcgacata gtcgggtttg gctgcaagcg ctgcgtgcag
      661 ctcttcaaca ttgtgagtgc tcacgccgag cccgatcccg gccgcgcgaa ttgctgatag
      721 gtcggcgttt gcgagatcct cctggccgag atgcacgaaa cgcgcgcctt gctcgatggc
      781 ctcacgccaa tagtcgttga tggcgagctg gcaaccatgc gcgcggcaaa gcgcgatggc
      841 cctcgcgatc tcatctttga cggccgcttc agacttgtcc ttgatgcgga gctgcaccag
      901 tttgatgccg tgcggcaaga gccgggcgag ccactccgtg tcggggagga tggggtagaa
      961 gggatcaagg ctgagcaaga ccggtctcgg atgacgaagc cggtcaggag gcgttgtcga
     1021 tcttgaagaa cggcgtgccg gcgacaggag tcgagggaga cgccatgtcg cgcggctcga
     1081 tcagccccgc ttcgtaggcg agccgccccg cctcgatggc gcgtgcgaag gcttgcgcca
     1141 ttttcaccgg atcgccagcc ttggcgacgg cagtgttgag cagcaccgcg tcatagccca
     1201 tctccatcga ctctgccgcg tgcgagggcg cgccaatccc ggcgtcgatg atgagcggcg
     1261 tgtcgggaaa ataagcgcgg agcgtcttga gcgcatcttt gttgatgagg ccgcgcgcgg
     1321 agccgatcgg cgcggcccag ggcatcagca ctttgcaccc cagggcgacg agacgctcgg
     1381 ccgcgctcag atcttcggtc gtatagggca ggacctcgaa gccttccttc gtaagctcag
     1441 ccgccgcctc gaccaatccg aagaggtcgg gctggagcgt gtcgtcgttg gcgatcactt
     1501 cgagcttgat ccacgaggtg ccgaacagct cgcgcgccat gttagccgtg gcgattgctt
     1561 ccttggcggt gcggcaccct gcggtgttcg ggagcacttg ggcgccgagt tgctcgatca
     1621 acgcccagaa gccttggccg gtccgggcac gggccgattc gcgcctgagc gagacggtga
     1681 cgatctccgc gcccgacgct tcaactgcct gcttcaggca ttcgggcgat ggatattgcg
     1741 cggtcccgag cagaagtctc gaggtgaagg tcgcgccgta gagagtgagc atcagccgcc
     1801 ctgcctcggc gccacgatct cgatctcggc ggcctcgatg agcggcgttc tcttgcgctc
     1861 cgaagccgcg acgaagctgc cgttcacggc ggtggcgatc ttggcatcgg cgaagcccaa
     1921 acctgcgcag agctcgtcga gatttctggc ctcggtcgcg aagcgcttac cgttgagcag
     1981 gatctgcaac aaacacctcc ggatgcgtct cgccggtcga aagataggtg gcgacaagct
     2041 cggccagagc cggcgcaagt aggaaaccgt gccggtagag gccgttgacg tagatatagc
     2101 ccttcgcagt gataatccta ggccgattgt cggggaaggc cggtcttgcc ccggcgccga
     2161 gcttaacgat ctccgcctcg ccgaaggcag ggtcgagggc ataggcggcc gacagaagct
     2221 caagcgccga tcgcacgctg accggacccg tctcctcgct ctcgaccacc gtcgctccga
     2281 tcatgaaaag gctctgcccc cagggcacgg catagagggg aaagcgggga tgaagcagcc
     2341 gcacgggccg cgagagggcg acatcgcgtg cacgcaccac gatgcgctcg ccacgcacgc
     2401 cgcgcagagt tggaagcttg tctctcgcgg ctagtccgcg gcaatcgatg atgacgtcgg
     2461 cgcttttcgg catttcgcct tcgccgagaa tgaggcgcgc gccggcggct cttgcctcag
     2521 ccagaaggaa gtcgagtgcc gcgtcagggg caagatgcgc ctcgccggga taatacagcg
     2581 cggtcgcata gcgtttggcg agatcgggct cgagcgctgc aagcgcatcg ccggtgagcc
     2641 gctctgagcc gtgcgtcatg cgcgcgaagc gatcgagcag ggtttggtcg cgcggcggcg
     2701 cgaccacgag cgagccgttg accacgatgc cgggataggt cgcgcgccaa agcgcgatgc
     2761 tgcgcagccc gagctcgccg atgacggctt cggcgctctc ctcctcgcag cgtggcgcga
     2821 gcatggcgcc ggcaaaaggg ctgcaggctt ccgcgaacgg cgtggtggag cgctcataga
     2881 tggtgaccgc gtgtcccgcc cgagcaagca ggagcgcctg ccaaagaccg acgacgcccg
     2941 cgccgcggat ggcgatcgat tgactgcggg cggccatcaa tctccgagcg ccacgccttc
     3001 acggcgcggg tcggccccgc cttcgaggcc gtctttcgtc accgcaatga tgtggagtcc
     3061 gctgacgaga tcggtgcgct ggacgttatg gccgagactc tcgagcgcgt tggcgaggct
     3121 gttccattcg gcgccgggtt cgatcaccac cgcatcgccg gtcgagccga aattgacaag
     3181 cgctgcggcg tcggccgcgt cgaggcgcca gtcgatcaga gcgatgagcg ccttgatgtt
     3241 atagaggatg atggcggcgc cgccggggct gccgagcaca taatgaagtg cgccgtcgcc
     3301 gttggcgaag acgagggtgg gatccaaaga gctccgcggg cgcttgcctc cttccacgcg
     3361 attggcgacc gggcttcccg cgttgtcgac ggggacaaac gagaagtcgg tgagctgatt
     3421 gttgagcagg aagccgcgca ccatgttgcg cgagccgaag gagcgctcga tggtcgtggt
     3481 catggagaag gcgttaccgt cgtcatctac gatcgagacc tgactggtgc catgggct
//
LOCUS       DATNAE010000236         3247 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_537926, whole genome shotgun sequence.
ACCESSION   DATNAE010000236 DATNAE010000000
VERSION     DATNAE010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537926"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..179
                     /locus_tag="VN518_05345"
     CDS             105..179
                     /locus_tag="VN518_05345"
                     /inference="COORDINATES: protein motif:HMM:NF014825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HXE04032.1"
                     /translation="MQRNALCPCGSGKKYKHCHGTFTS"
     gene            complement(218..1057)
                     /locus_tag="VN518_05350"
     CDS             complement(218..1057)
                     /locus_tag="VN518_05350"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase family protein"
                     /protein_id="HXE04033.1"
                     /translation="MREFLSLLILAVLIGAVLFALAPEGRQTLDRVRINATRAEHQAR
                     YHAGEPLAGTPDLAHLDERLAAQGAKLGDPVMLRLFKLESQLELWVEKDGRFVRFATY
                     PICLWSGRLGPKIKEGDRQAPEGFYTIAAEQLNPNSRWHRAFNLGFPNEFDKAKGRDG
                     SFIMVHGGCSSIGCYAMTDAVVDELWRFVTAALDAGEGRVPVHVFPFRMTDANLQARR
                     SDQWAGFWTDLKKGHDLFERTQMPPVVSVCNGRYAFSPGSATTVGSAIEERCPPEVAT
                     NQG"
     gene            complement(1145..2128)
                     /locus_tag="VN518_05355"
     CDS             complement(1145..2128)
                     /locus_tag="VN518_05355"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase family protein"
                     /protein_id="HXE04034.1"
                     /translation="MLRSGKVTAVLAASVVILVTALLGGCGQFAPSYMKPLSAQTRAL
                     LAEKGMTEDAPILIRIFKAESELEVWKAKDDGRFHLFKTYPICSYSGGLGPKVSQGDR
                     QAPEGFYLVSQEQMNPNSKYHLSFNVGFPNAYDRAYGRTGQDIMVHGDCTSAGCYAMT
                     DGVIEEIFILAYQALQGGQEAFQIQAYPFRMTAANMTAHQKDEWYDFWKNLKEGYDYF
                     EITHLPPRIAVCEKRYLVNAAFVDRGAHPDPAGACPAWQRLPVSRAPAEQVASTKKTN
                     EAALTKPLAMVLGLSLGPQKPVYRAFTLGPATPVSTPAPAPAPAKSAAKGK"
     gene            complement(2301..>3247)
                     /locus_tag="VN518_05360"
     CDS             complement(2301..>3247)
                     /locus_tag="VN518_05360"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HXE04035.1"
                     /translation="YLDFEKPIAELESKVAELKALAAEQRSVSIEEELAKLEIKAKEA
                     LEETYAELTPWQKTQVARHPERPHFRHYIDGLVEGFTPLSGDRAFADDQAIIGGIGRL
                     SGRSAMIIGHEKGSDTEGRIKHNFGMARPEGYRKAVRLMEMADRFSLPVVSLVDTAGA
                     YPGIGAEERGQAEAIARSTDCCLSLGVPIISVIIGEGGSGGAVAIATANRIYMLEHSI
                     YTVASPEAAASILWKDSTRAVDAATNMKITAQDLLKLGIIDGIIPEPIGGAHRDGVEV
                     VRAAGDTIVAAIAEFDDLSPEEIRKDRREKFLAMGRAS"
BASE COUNT          552 a         1044 c         1043 g          608 t
ORIGIN      
        1 gcaaggccgc gagcggggcc gctgcgggcg cccggaaggc ggcgggccct gccagtgccg
       61 ccgccgctgc ggtcaacccc gccgatccct cgacgtgggg caaggtgcag aggaacgcgc
      121 tttgcccgtg cggttcaggc aagaaataca agcactgcca cggcacgttt acgagctagg
      181 ggccgtaatc ccgccgcagc ctcgcggctt gcggttctca gccttgattg gttgcgactt
      241 cgggcgggca gcgctcctcg atggcgctgc cgacggtcgt ggcacttccc ggcgaaaacg
      301 cgtagcgccc gttgcatacg ctgacgaccg gcggcatttg cgtccgctcg aacaggtcgt
      361 ggcctttctt gagatcggtc cagaagccag cccattgatc cgaccggcgc gcttgcagat
      421 tggcgtcggt catgcggaac gggaacacgt gcaccggcac cctcccctcg cccgcatcga
      481 gcgccgccgt gacaaaacgc caaagctcgt cgaccaccgc gtcggtcatg gcatagcagc
      541 cgatcgacga gcatccgcca tgcaccatga tgaaggagcc gtcacgccct ttcgctttgt
      601 cgaactcgtt ggggaaaccg agattgaagg cacggtgcca gcggctgttc ggattgagct
      661 gctccgccgc gatcgtatag aagccctcgg gcgcctggcg gtcgccttcc ttgatcttgg
      721 gtccaagccg tcccgaccac aggcatatgg ggtaagtggc gaagcgcacg aaacgcccgt
      781 ccttctccac ccaaagctcg agctgtgact cgagcttgaa caggcggagc atgaccggat
      841 cgccgagctt cgccccttgc gccgccagtc tctcgtcgag atgggcgaga tcgggggtgc
      901 cggcgagagg ctcacccgcg tggtagcgtg cctgatgctc ggcgcgcgtg gcgttgatgc
      961 gcacgcggtc gagagtttgc cgcccctccg gtgcgagcgc gaacagcacg gcaccgatca
     1021 gaacagcgag gatgaggagc gacaagaatt cccgcattcg cgtccagaag tcagcggtcc
     1081 gagatcagcg atcgtaagtc agaatgagcc cctgtggttt cagatttctg acttctgctg
     1141 tccgtcactt gccctttgcc gcgctcttcg cgggggctgg cgccggcgcc ggcgttgaaa
     1201 ccggcgtcgc cggaccgagc gtgaaggcgc gatagaccgg cttttgcggg ccaaggctca
     1261 ggcccaacac catggcgagc ggcttggtga gagccgcttc gttcgtcttc ttggtcgagg
     1321 ccacctgctc ggccggcgcg cgcgacaccg gcaatctctg ccaagcggga caggcgcctg
     1381 cgggatcggg atgcgcgccg cgatcgacga aggcggcatt gacgagatag cgcttctcgc
     1441 acaccgcgat gcgcggcggg agatgcgtga tctcgaaata gtcgtagcct tctttcaggt
     1501 tcttccagaa gtcgtaccat tcgtccttct gatgcgcggt catgttcgcc gccgtcatgc
     1561 ggaacggata agcctggatc tggaaggcct cctgcccccc ctggagcgcc tgatacgcaa
     1621 ggatgaagat ttcctcgatg accccgtccg tcatggcgta gcagcccgcc gacgtgcagt
     1681 cgccatgcac catgatgtcc tgcccggtgc ggccataggc gcggtcatag gcgttgggga
     1741 agccgacatt gaacgacaga tggtacttgc tgttcgggtt catctgctcc tgcgagacga
     1801 gatagaaacc ttccggcgcc tgccggtcgc cctggctgac cttggggccg aggccgccgg
     1861 aataggagca gatcggatag gttttgaaga gatggaagcg gccgtcgtcc ttggccttcc
     1921 acacttcgag ctccgactcg gccttgaaga tgcggatgag gatgggcgcg tcttcggtca
     1981 tgcccttttc ggccagcagc gcccgcgtct gcgcggacag cggcttcatg taggagggcg
     2041 cgaactgccc gcagccgccc agcagcgcgg tgaccaaaat caccacgctt gccgcgagaa
     2101 cggccgtgac cttcccagat cgaagcatac gcgagtgccc cttgcgcatc tcggtcaaga
     2161 tcgaatctgc gaggttaaag cgaagttagc gctgctcacg cgcgcttttc cttcggtttc
     2221 ccccttttag ctcccattat aagctttccc gactctcgtt tggacggaaa aaatgtcgct
     2281 ttttggggca gatttggccg ctagctggcg cggcccatag ccaggaactt ctcgcgccgg
     2341 tccttgcgga tctcctctgg cgagagatcg tcgaactcgg cgatcgcggc gacgatcgta
     2401 tcgcccgcgg cgcgcaccac ctcaaccccg tcacgatggg ctccgccgat cggctctgga
     2461 atgattccat cgataattcc gagctttagc aggtcctggg cggtgatctt catattcgtc
     2521 gcggcatcga ccgcccgcgt cgaatccttc cataggatcg aggctgcggc ttccggcgag
     2581 gcgaccgtgt agatcgaatg ctcgagcata tagatccggt tcgccgtggc gatggcgacg
     2641 gcgccgcccg agccgccctc gccgatgatg acggagatga tcggcacgcc gaggctgagg
     2701 cagcaatcgg tcgagcgggc aatggcctcg gcctgccccc gctcctcggc gccgatgcca
     2761 gggtaggcgc ctgccgtatc gaccaggctg accacgggga gcgagaaccg gtcggccatc
     2821 tccatcagcc gcacggcctt gcggtaccct tccggccgcg ccataccgaa attgtgcttg
     2881 atgcggcctt cggtgtcgga gcccttctcg tggccgatga tcatcgccga acggccggac
     2941 agccggccga tgccgccaat gatggcctgg tcgtcggcga aggcgcggtc gccggagagc
     3001 ggcgtgaagc cttccaccag cccgtcgatg tagtggcgga aatgcggacg ctcgggatgc
     3061 cgcgccacct gcgtcttctg ccagggcgtg agctcggcgt aggtctcctc caaggcctcc
     3121 ttggccttga tctcgagctt ggcgagctcc tcctcgatcg agaccgagcg ctgttcggcg
     3181 gcaagcgcct tcaactcggc gaccttgctt tcaagctcgg cgatcggctt ttcgaaatcg
     3241 agataag
//
LOCUS       DATNAE010000237         7038 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3242253, whole genome shotgun sequence.
ACCESSION   DATNAE010000237 DATNAE010000000
VERSION     DATNAE010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 7038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7038
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3242253"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1689
                     /locus_tag="VN518_05365"
     CDS             <1..1689
                     /locus_tag="VN518_05365"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HXE04036.1"
                     /translation="FPFIPNFGFTLGLELVREGSLRTMHALMPIPSQLTRFIQLPSGG
                     GSEGAIRFIRLETVVGIFVSRLFPGYRVRSQGAFRVLRDSEVEVQEEAEDLVRLFESA
                     LKKRRRGSVIRLEIEASMPARLQRFVMKELKVSDRESFVQKGLIGVTDTAQLIAADRP
                     DLKFQPYLSRFPERIREHNGDCFGAIREKDIIVHHPYESFDVVLQFLKQATADPDVVA
                     IKWTLYRTSKTSPIIEALKDAVEAGKSVTAVVELKARFDEEANIKWARDLESAGVQVV
                     YGFIELKTHAKLSLIVRREGGDLVTYCHVGTGNYHPVTAKIYTDLSFFTAERTIGHDV
                     SRIFNFVTGYAEPAELEAMAVSPHGIRARILDHIREEMYHAKAGRPAAIWMKMNSLVD
                     AEIIDALYEASQAGVEIDLVVRGICCLRPGVPHLSETIHVKSIVGRFLEHSRIFCFGA
                     GHGLPSRNAIVYISSADMMPRNLDRRVEAVAPIKNPTVHEQILDQIMVANLKDNVQSW
                     RVRPDGSSERIRLGMGQEPFSAQDYFMTNPSLSGRGTSLKENMPPRFARLVESS"
     gene            1712..3229
                     /gene="ppx"
                     /locus_tag="VN518_05370"
     CDS             1712..3229
                     /gene="ppx"
                     /locus_tag="VN518_05370"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HXE04037.1"
                     /translation="MARRNELSPVAVVDIGSNSVRLVVYEGATRAPTPLFNEKVLCGL
                     GRSIATTGRLGADAVARALRALRRFRALIDQMGVSRVEAIATAAAREAENGPEFIAQA
                     ERIIEVPIRVLTGTKEAELAAVGVIAGIHDADGFVGDLGGGSLELIDVRKGKLGDAAT
                     LPLGGLRLIDASGGNLKRAREIVDAELAKVGWLDKGKDRDFYAIGGTWRALARLHMTQ
                     TNYPLNVMHNYRIDVAEALKFASLLDQQSPSSLAGIRDISGARRETIPYGSLVLERLI
                     RQVKPRAVVISVFGIREGLLCTMLSEEERRKDPLLAACDDLARRRSRSIDSAYELCFW
                     TDALFRAPGPTETPEQRRLRHAACLLSDIGWHAHPDYRGIQSLNLVAHGNFVGVDHPG
                     RAFLALTVYYRNEGTLKDELSQSLIELVDKDMLKRARILGAAFRAAHMVSASMPGVLT
                     NTPIGYEGNRLVWTLPDPYSNLEGERVDRRFKVLADLLDREAEIRVGPSFQLAAS"
     gene            3338..4270
                     /locus_tag="VN518_05375"
     CDS             3338..4270
                     /locus_tag="VN518_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HXE04038.1"
                     /translation="MADDLYSVLGVARTAKAEDITKAYRKLAKKLHPDLNPGDKAAED
                     KFKQVTAAYDILGDAEKRRRFDAGEIDASGQERPQQRYYREYAGGEDGARYRSTAGYE
                     DLGGSSDLFGDLFGRGGGARGAGGQRFAMRGQDAQYRLEIDFLEAVNGAKKRITLPDG
                     GTLDVTIPAGVEAGQVMRLKGKGMPGLGGAESGDALVEILVKPHPVFKREGDDIVVEV
                     PISFDEAVLGGKVEVPTITGNVFATVPPGTNTGKTLRLKGRGIKRKNGKLGDELVKLT
                     VVMPDRIDEDLKAFAEEWRGKHRYDPRRKHREQA"
     gene            4270..4605
                     /locus_tag="VN518_05380"
     CDS             4270..4605
                     /locus_tag="VN518_05380"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HXE04039.1"
                     /translation="MLSERDIVSRVQHVTVTRLRVWVRQGWIRPAAKESQGFSEADMA
                     RAALIRDLEDKLGFDEEQVPVLLNLIDQIHGLRAELKTMLDALEALPEEVRATVKMRI
                     TKRKPKSRG"
     gene            4602..4802
                     /locus_tag="VN518_05385"
     CDS             4602..4802
                     /locus_tag="VN518_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014762868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HXE04040.1"
                     /translation="MTRTALVVAAFIAAAGLLTFASVRFGLGRLPGDIVIDRGGFVLY
                     LPITTSILVSLLLSLILWLVNR"
     gene            complement(4764..5939)
                     /gene="rnd"
                     /locus_tag="VN518_05390"
     CDS             complement(4764..5939)
                     /gene="rnd"
                     /locus_tag="VN518_05390"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HXE04041.1"
                     /translation="MHLVTRTDDLAEACARLGAAPFVAVDTEFMREQTFWPRLCLIQM
                     AANGTEVLVDSLAPGIDLQPFFDLMVNEQVLKVFHSARQDIEIVHHLAGVIPRPIFDT
                     QVAAMVCGFGEAVSYSMLVKRLLSRNLDKTSRFTDWSRRPLSERQYTYALGDVEHLRD
                     LYPKLRSQLDRSERASWLNEEMAVLTDPATYELHPEHAWKRLKMRIKTPKSLAVLMEL
                     AAWREREAQTQDVPRARILKDEALYDIASQAPRTTEDLGSLRTLHNGFARSMRGRAVL
                     EAVSKGLERDPKTIPPIERGEPMPPEAQAVVDLLRVLLKATAGKHGVAPKLIATSDEL
                     EEIARSDDIDVPVLRGWRRKLFGDDALALKRGELALIIRKGEVTVLPIDQPKNERKQ"
     gene            6476..>7038
                     /locus_tag="VN518_05395"
     CDS             6476..>7038
                     /locus_tag="VN518_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012456189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HXE04042.1"
                     /translation="MHPYRTKTCGELRKENVGQTVRLSGWVHRVRDHGGLLFIDLRDH
                     YGITQCVVDPASPAFAEAEKLKPEWVVRVDGEVVARSAETINPTLPTGEVEVRAKTIE
                     VLSEAKELPLPVFGEPDYPEETRLKYRFLDLRRESLHANIMKRQAIIWSLRHRMREAG
                     FFEFQTPILTASSPEGARDYLVPSRVHP"
BASE COUNT         1282 a         2243 c         2254 g         1259 t
ORIGIN      
        1 tttcccttca ttcccaattt cggcttcacg ctggggctcg agctcgtgcg cgaggggtcg
       61 ttgcgcacca tgcacgctct gatgccgatc ccctcgcagc tcacgcgctt catccaactg
      121 ccttcgggcg gagggagcga gggcgcgatc aggttcatca ggctcgaaac cgtggtcggc
      181 attttcgtct cgcgcctgtt cccgggctat cgggtcagga gccagggcgc gttccgcgtg
      241 ctgcgcgaca gcgaagtcga ggtgcaggaa gaggctgagg atctcgtgcg cctgttcgaa
      301 tcggcgctga agaagaggcg ccgcggctcg gtgatcaggc tcgagatcga ggcctcgatg
      361 ccggcacgcc tccagcgctt cgtgatgaag gagctcaagg tctccgaccg cgagagcttc
      421 gtgcaaaagg ggctcatcgg cgttaccgac acggctcagc tcatcgccgc cgatcgcccc
      481 gatctcaagt tccagcccta tctcagccgc ttcccggagc gcatccgcga gcataacggc
      541 gactgcttcg gcgccatccg cgagaaggac atcatcgtcc atcacccata cgaatccttc
      601 gatgtggtgt tgcagttctt gaagcaggcg accgccgatc ccgacgtcgt ggccatcaaa
      661 tggacgctct accgcacgag caagacctcg ccgatcatcg aggccctgaa ggacgcggtg
      721 gaagccggga agtcggtcac cgccgtggtc gagctcaagg cccgcttcga cgaggaagcc
      781 aacatcaaat gggcgcgcga tctggaaagc gcgggcgtgc aggtggtgta tggcttcatc
      841 gagctcaaga cccacgccaa gctgagcctg atcgtgcggc gcgagggtgg cgatctcgtc
      901 acctactgcc atgtcggcac cggcaattat cacccggtca ccgccaagat ctacaccgac
      961 ctgtcattct tcaccgccga gcgcaccatc ggtcacgatg tgtcgcgcat cttcaatttc
     1021 gtcacgggct atgccgagcc ggccgagctt gaggccatgg cggtctcccc gcacggcatc
     1081 agggcgcgca tcctcgacca tatccgcgag gagatgtatc acgccaaggc tgggcgcccg
     1141 gcggcgatct ggatgaagat gaactcgctc gtcgacgccg agatcatcga tgcgctctac
     1201 gaggcaagcc aagccggtgt ggaaatcgat ctcgtagtgc gcggcatctg ctgcctgcgg
     1261 cctggcgtgc cgcatctctc cgagaccatc cacgtgaaga gcatcgtcgg gcgcttcctc
     1321 gagcattcac gcatcttttg cttcggcgcc ggccacggct tgccgagccg caacgccatc
     1381 gtctatatca gctcggccga tatgatgccg cgcaacctcg atcgccgggt ggaggccgtg
     1441 gccccgatca agaacccgac ggtgcacgag caaatcctcg accagatcat ggtggcaaac
     1501 ctcaaggaca atgtgcagag ctggcgggtg aggccggatg gaagttcgga gcgaatccgg
     1561 ctagggatgg gacaggaacc attcagcgcg caggattatt tcatgaccaa tccgagtctc
     1621 tcgggccgcg gcacttctct caaagagaac atgccgccgc gcttcgcgcg cttggtcgaa
     1681 tctagttgaa gtccggcgag tagcccccgt catggcgcgc agaaatgagc tgagccccgt
     1741 cgccgtggtc gacatcggtt cgaactcggt gcggctcgtg gtctatgagg gcgcgacgcg
     1801 ggcgcccacc ccgctgttca acgaaaaggt gctgtgcggc ctggggcgct cgattgccac
     1861 cactgggcgg cttggcgctg acgccgtggc gcgtgcgctg cgggcgcttc gccgctttcg
     1921 cgcgctgatc gaccagatgg gcgtgagcag ggtcgaggcg atcgccacgg cggcggcgcg
     1981 cgaagccgag aacgggcctg agttcattgc tcaagccgag cgcatcatcg aagtgccgat
     2041 cagggtgctg accggcacca aggaggctga gctcgcggcc gtcggcgtca tcgccggcat
     2101 tcacgacgcc gacggcttcg tcggcgatct cggtggcggc agtctcgagc tgatcgacgt
     2161 gcgcaaaggc aagctcggcg atgccgcgac cttgccgctc ggcggcctgc ggctgatcga
     2221 tgcctcaggc ggcaatttga agagggcgcg agagatcgtc gacgccgagc tcgccaaggt
     2281 cggttggctg gacaagggca aggaccgcga cttctacgcc atcggcggaa cgtggcgcgc
     2341 gcttgcgcgc ctgcacatga ctcagaccaa ttatccgctg aacgtcatgc acaattatcg
     2401 catcgacgtc gccgaggcgc tcaaattcgc gagcctgctc gaccagcaat cgccaagctc
     2461 gctggccggc atccgggaca tctccggcgc gcggcgcgag accatcccct acggctcgct
     2521 ggtgctcgaa cggttgatca ggcaagtgaa gcctcgcgcc gtggtgatct cggtcttcgg
     2581 catccgcgag ggtctgttgt gcaccatgct gagcgaagag gagcggcgga aggacccgct
     2641 gcttgccgct tgcgacgatc tcgcccgccg ccgctcacgc tccatcgact cggcctatga
     2701 gctttgcttc tggacagatg cgctgttccg cgcgcccgga ccaaccgaga ccccggagca
     2761 gcgaaggctg cgccatgccg cctgcctcct gtccgacatc ggctggcacg ctcatccgga
     2821 ttatcgcggc attcagagct tgaacctcgt ggcccacggc aatttcgtcg gggtggatca
     2881 tccgggccgc gcgttcctcg cgcttaccgt ctactatcgc aacgaaggca cgctgaaaga
     2941 cgagctgagc caaagcctga tcgagctggt cgacaaggac atgctgaagc gcgcgcgcat
     3001 ccttggcgcc gcgttccgtg ccgcccatat ggtgtcggcc tcgatgcctg gcgtgctcac
     3061 caacacgccg atcggctatg agggcaaccg gctggtctgg accttgcctg atccctattc
     3121 caacctcgaa ggggagcgcg tcgaccgcag gttcaaggtg ctggccgacc tgctcgaccg
     3181 ggaagccgaa atccgcgtcg gcccctcatt tcaactcgcg gcaagctgag tctcaacttg
     3241 ctggacgagg ccgcttttcc caaatcatat gctatgagaa aaggcgccaa cctttggcgc
     3301 cttcagaagc ccaccgttag gccgctaagc tcctagaatg gcagacgatc tctattcagt
     3361 tctcggcgtg gcgcgcaccg cgaaggctga ggacatcacc aaggcctatc gcaagctcgc
     3421 caagaagctg catcccgacc tcaatccggg agacaaggcg gccgaggaca agttcaagca
     3481 ggtgactgcc gcctacgaca ttctcggcga tgccgaaaag cgccgccgct ttgacgctgg
     3541 cgagatcgac gcgagcgggc aggagcgtcc gcagcagcgt tactatcgcg aatatgccgg
     3601 cggggaggat ggagcacgct accgctcgac agcgggttac gaggatcttg gcggctcctc
     3661 cgatctgttc ggcgatctgt tcggcagggg aggcggtgcg cgcggcgccg gcggtcagcg
     3721 cttcgccatg cgcgggcagg acgcgcaata ccggctcgag atcgatttcc tcgaggcggt
     3781 gaacggcgcc aagaaacgca tcacgctccc cgacggcggc accctcgacg tgaccattcc
     3841 ggccggcgtc gaggcaggtc aggtgatgcg gctcaaaggc aagggcatgc ctggccttgg
     3901 cggcgccgag tcgggcgacg ctctggtcga gatcctggtc aagccgcacc cggtgttcaa
     3961 gcgcgagggc gacgacatcg tggtcgaggt gccgatcagc ttcgatgagg ccgtgctcgg
     4021 cggcaaagtc gaggtgccca ccatcaccgg taacgtcttc gccactgtcc cgcctggcac
     4081 caacaccggc aagacgctgc ggctgaaggg ccgcggcatc aagcgcaaga acggaaagct
     4141 tggtgacgag ttagtcaagc tcaccgtggt gatgccggac aggatcgatg aggatttgaa
     4201 ggcctttgcc gaagaatggc gcggcaagca ccgttacgat ccgcgccgca agcatagaga
     4261 gcaggcgtga tgttgagcga acgcgacatc gtgtctcgcg tgcagcacgt caccgtgacg
     4321 cgcttaaggg tctgggtgcg gcaaggctgg atcaggcccg ccgccaagga gagccaaggc
     4381 ttctccgaag ccgatatggc gcgcgcggcg ctgatccgcg acctcgaaga caagctcggc
     4441 ttcgacgagg agcaggtgcc ggtgctgctc aacctgatcg atcagatcca tgggctgcgc
     4501 gccgagttga aaaccatgct cgatgcgctc gaggccttgc ccgaagaggt gcgcgccacg
     4561 gtgaagatgc gcataaccaa gcgcaagccg aagtcccgcg gatgacgcgc accgctcttg
     4621 tggtggccgc ctttatcgcc gccgctggtc tcttgacctt tgccagcgtg cgcttcggtc
     4681 tcggcaggct tcccggcgac atcgtcatcg accgtggtgg cttcgtgctc tacctgccga
     4741 tcaccacctc aatcctcgtg agcctactgc tttcgctcat tctttggctg gtcaataggt
     4801 agcaccgtca cttcgccctt gcggatgatc agggcaagct cgccgcgctt caaggcaagc
     4861 gcgtcgtcgc cgaacagctt ccgccgccag ccgcgaagaa cgggcacgtc gatgtcatcg
     4921 gagcgggcga tctcttccag ctcgtcgctg gtcgcgatca gcttcggcgc cacgccatgc
     4981 ttgccggcgg tcgccttgag cagcacgcgc aggaggtcga ccacggcttg cgcctccggc
     5041 ggcatcggct caccgcgctc gatcggcggg atggtctttg gatcgcgctc gagccccttg
     5101 gataccgcct cgagcacggc gcggccgcgc atggaacggg cgaagccgtt atgcaaggtc
     5161 ctgagcgagc ccaggtcctc ggttgtgcgc ggcgcttggc tcgcgatgtc ataaagcgcc
     5221 tcatccttca ggatgcgggc gcgcggcacg tcctgggtct gcgcctcgcg ctcgcgccag
     5281 gcggcgagtt ccatgagcac ggcgagagac ttcggcgtct tgatgcgcat cttgagccgc
     5341 ttccaggcgt gctcgggatg cagctcgtag gttgcagggt cggtgagcac cgccatctct
     5401 tcgttgagcc agcttgcgcg ctcggaccgg tcgagctggc tgcggagctt ggggtagaga
     5461 tcgcgcagat gctcaacgtc accgagcgca taagtgtact ggcgctcgga gagggggcgg
     5521 cggctccaat cggtgaagcg cgaggtcttg tccagattgc ggctcaacag ccgtttcacc
     5581 agcatcgagt agctcaccgc ctcgccgaag ccgcagacca tggcggcaac ttgcgtgtcg
     5641 aagatcggac gcgggatgac ccccgccaaa tgatggacaa tttcaatgtc ttggcgggcc
     5701 gaatggaaga ccttgagcac ctgctcgttg accatcagat cgaagaaagg ctgaaggtcg
     5761 attcccggcg cgaggctgtc caccagcacc tcggtgccgt tcgccgccat ctgaatgaga
     5821 cagagccggg gccaaaaggt ctgctcgcgc atgaactcgg tgtccaccgc cacaaaaggc
     5881 gcagcgccaa gccgcgcgca agcctcggcc aggtcatcgg tacgggtaac gagatgcatc
     5941 gggtgcggat atagcttaag gcacggcttg aggccaagtg cgccgatcga cgtcaatcga
     6001 cttaccccgt actttgatcg ccgggctccc tacggtttgg tagcgccgac cgtagcgagc
     6061 cggggcagca gttgtgtgac gcggttactg gcggaggcgg cgatgggccg cggtgctcat
     6121 ggctcttggc taagccgatc gctcaaacac gcgcttgttg cgatggctta agctgatcgg
     6181 tcgcttgctc gatgctcggc cgcgcgtgct cggcgaccga catgtgcaag agcgaggaca
     6241 ccaacgagaa gagtgccacg agccccgcga gcgcccaggc ctcaaggggc aaacgcgttc
     6301 ttacgggaag gctcccaaca atcctaaaca ttgaaattct ccctcaatgt gccgcatggc
     6361 caataaactt tgctgctctc gctttgttcc gaagccgccg cttgccttga caaggcgggt
     6421 cgtcccatgc gctgttccgg cgcgatcctc ccatcccact tttgtccttt cagtcatgca
     6481 cccctatcgc accaaaacct gtggcgagtt gcggaaagag aatgtcggcc agaccgtcag
     6541 gctctccggc tgggtgcacc gcgtgcgcga ccatggcggg ttgctgttca tcgatctccg
     6601 cgaccattac ggcatcaccc aatgcgtggt ggacccggcc tcgccggcct ttgccgaggc
     6661 ggagaagctg aagccggaat gggtggtgcg cgtcgatggc gaggtcgttg cccgctcggc
     6721 cgaaaccatc aatccaactc tgccgacggg agaggttgag gtcagggcca aaacgatcga
     6781 ggtgctgtcg gaggcgaagg agctccctct gccggtgttc ggcgagcccg actatccgga
     6841 ggagacgcga ctcaaatacc gcttcctcga tctccgccgc gagagcctgc acgccaacat
     6901 catgaagcgc caggcgatca tctggtcgtt gcggcaccgc atgcgcgagg ccggcttctt
     6961 cgagtttcag acgccaatcc tcaccgcctc ctcgcctgag ggggcgcgtg actacctcgt
     7021 gccgagccgc gtgcatcc
//
LOCUS       DATNAE010000238         3207 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_4452114, whole genome shotgun sequence.
ACCESSION   DATNAE010000238 DATNAE010000000
VERSION     DATNAE010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4452114"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="VN518_05400"
     CDS             <1..582
                     /locus_tag="VN518_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711141.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HXE04043.1"
                     /translation="LAAEARDCTRCHLYKNATQTVFGEGPADAALMLIGEQPGDQEDL
                     AGRPFVGPAGKVLDRALEAAGIEREAIYITNAVKHFKNEPRGKRRLHKKPDTSEIDAC
                     RWWLDNELALIEPAVAVALGATAARALLRKALAINANRGRLIALPNGGNALITVHPSY
                     LLRLQEARDKRREFDLLVKDLHLAAEAARKGRG"
     gene            complement(590..928)
                     /locus_tag="VN518_05405"
     CDS             complement(590..928)
                     /locus_tag="VN518_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007559495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HXE04044.1"
                     /translation="MKYIIAVIQPHRLEAVREALADIGVQGLTVSEVRGFGRQKGQTE
                     VYRGAEYTMQYVPKVKLEIAVSASLADSVVEALGNAARTGHIGDGKVFVLPLEGAMRV
                     RTGEMGADAL"
     gene            1269..1733
                     /gene="greA"
                     /locus_tag="VN518_05410"
     CDS             1269..1733
                     /gene="greA"
                     /locus_tag="VN518_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HXE04045.1"
                     /translation="MSKAFTKEAEGGEVYDDLPDRPVSPHNLVTPKGLEMIEAELARL
                     HLEHAAAQDADDRPRLAKINRDLRYWTSRRATAQVAEPPRDAAQVHFGSTVTIERDDG
                     RRQTYRIVGEDEADPSLGTLSYVSPLAQALMGKEKGETVQAGVAEATIVGIT"
     gene            complement(1736..2059)
                     /locus_tag="VN518_05415"
     CDS             complement(1736..2059)
                     /locus_tag="VN518_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HXE04046.1"
                     /translation="MSQPVVVTIPHKLGKQEAARRLKAGFGNVRSTFGEKFVVLKDDW
                     AGDHLDFRASLLGQTTTGTVDVADDHVRLEVQLPWMLSLLANKAKALVQKQGKLMLEK
                     PTKPT"
     gene            complement(2197..2775)
                     /locus_tag="VN518_05420"
     CDS             complement(2197..2775)
                     /locus_tag="VN518_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE04047.1"
                     /translation="MKFAYFSLAAILAALVLGASLSLPASAGNDLHASAVHACTIANA
                     SQPVDFVAEVDDGRGGSLVWLTDAEANLWLCNADADGQIFAYSKMTGDLLLGAGANLV
                     GIEPVVNGEVGTPEKNPLTIAEKACQAYLSDGPGKVVGSGPDGLDGDWVPGYYVYIDT
                     GKGLVLCDATADAQVWSFAEIDNPLSLGNAVG"
     gene            complement(2896..3192)
                     /locus_tag="VN518_05425"
     CDS             complement(2896..3192)
                     /locus_tag="VN518_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006937468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SelT/SelW/SelH family protein"
                     /protein_id="HXE04048.1"
                     /translation="MPPAPNVAITYCRLCNWLLRAAWMQQELLSTFAEELGSVTLIPD
                     DTGGVFEIKIDGKLIWSRGERGRFPEIKELKQIVRDEIAPERELGHVEKKEDQT"
BASE COUNT          575 a         1047 c         1035 g          550 t
ORIGIN      
        1 ctggcggcgg aagcgagaga ttgcacgcgc tgccatctgt acaagaatgc gacccagacc
       61 gtgttcgggg aaggccccgc cgatgcggcg ctgatgttga tcggcgagca gccgggcgat
      121 caagaagacc tggccggacg ccccttcgtg ggaccggccg gcaaggtgct tgaccgagcg
      181 ctcgaggccg cgggaatcga gcgcgaagcg atctacatca ccaacgcggt gaagcacttc
      241 aagaacgagc cgcgcggcaa gcgacgcctg cacaagaaac ccgacaccag cgagatcgac
      301 gcctgccgct ggtggctcga caacgagctc gcgctgatcg agcccgccgt ggctgtggcg
      361 ctcggggcga ccgcggcccg cgcgctgctg cgcaaggcgc ttgccatcaa cgccaatcgc
      421 ggccgactca tcgccctgcc gaacgggggc aatgccctca tcaccgtgca cccgtcttat
      481 ctgctccgcc tgcaagaggc gcgcgacaag cgccgcgagt tcgatctctt ggtcaaggat
      541 ttgcacttgg ccgccgaggc ggcgcgcaag gggcgaggct gatccccgcc tagagcgcgt
      601 cggcgcccat ctcgccggtg cgcacgcgca tcgccccttc gagcggaagc acgaacactt
      661 tgccgtcgcc gatatgcccg gtgcgcgcgg cgttgccgag cgcctcgacc acgctgtcgg
      721 caagcgaggc gctcacggcg atctcgagct tcaccttcgg cacatattgc atggtgtatt
      781 cggcgccgcg atagacctcg gtctggccct tctgccgccc gaacccgcgc acctcgctga
      841 ccgtcagccc ttgcacgcca atatcggcaa gcgcctcgcg cacggcctcg agccggtggg
      901 gttgaatcac tgcaatgata tatttcatgt cgctcgtcct cgatcccatg ttgtagccgc
      961 gctcgccgtg gagcctgaga tcgaggccct gttcctcggt ctcggcaccg aggcgtaatc
     1021 ctgtgaccgc atagacgacc tttggcgata acgaagcttg gcaccgcgac catagcaaag
     1081 aggcggcgat gccagcgagt tgaattaccg cctcttacgc ggcggacgcc ttcggataaa
     1141 tttcgccgtt taccaaaatc ccggatcaag ggagcatcag cgaaggcttg ccttattcgg
     1201 cgtatcaccc cgcccgcgag ggggagatgc aaccacaatc ccaaggcgcg gcgtggtata
     1261 gtgctttgat gagcaaggct ttcaccaagg aagccgaagg cggagaggtc tatgacgacc
     1321 tgccggaccg cccggtttcg ccgcataatc tcgtgacgcc caagggactc gaaatgatcg
     1381 aggccgagct tgcgcggctg catctcgagc acgcggcagc tcaagacgcc gacgaccggc
     1441 cgcggcttgc caagatcaat cgggacctgc gctattggac ctcgcgccgg gcgaccgcgc
     1501 aggtggccga accgccgcgc gacgcggccc aggtgcattt cggctcgacg gtgacgatcg
     1561 agcgcgacga cgggcgccgc caaacctacc ggatcgtcgg cgaggacgag gccgacccct
     1621 cgctcggcac gttgtcctac gtgtcgccgc tggcgcaagc cttgatggga aaagagaagg
     1681 gcgagacggt gcaggccggc gtggccgaag ccaccatcgt cggcattacc tagccctagg
     1741 tcggcttggt cggcttttcc agcatcagct tgccttgctt ctgcaccagg gccttggcct
     1801 tgttggccag cagcgacagc atccagggaa gctgcacttc gagccgcacg tggtcgtcgg
     1861 cgacatccac cgtgccggtg gtggtttgcc caagcaggct cgcgcggaaa tcgagatgat
     1921 cgccggccca gtcgtccttc agcaccacga atttctcgcc gaaggtcgaa cgtacattgc
     1981 cgaagccggc tttcaagcgg cgcgcggcct cttgcttgcc gagcttgtgc gggatggtga
     2041 cgacgacggg ttgggacatc ttgccgtagc tctcctggcg aaccggatcg ttcccctcat
     2101 gtagggagag agcgccagag gtccagaggc tcgcgcaaga atgccaaggg gcgcaagagc
     2161 ctgaagccct tgcgccccgg tctcagatga gctggctcag ccgaccgcat tgccgaggct
     2221 gagagggttg tcgatctcgg cgaagctcca gacctgcgcg tccgccgtgg catcgcacag
     2281 cacgaggccc ttgccggtat cgatgtacac gtaatagccg ggcacccaat cgccatcgag
     2341 gccgtcgggg ccggagccca cgactttgcc ggggccgtcc gagagataag cctggcaggc
     2401 cttctcggcg atggtcagcg ggttcttctc gggcgtgccg acctcgccat tcaccaccgg
     2461 ctcgatgccg acgagattgg cgccggcgcc gagcaacaaa tcgccggtca tcttgctgta
     2521 ggcgaagatc tgcccgtcgg cgtcggcgtt gcagagccag agattggctt cggcatcggt
     2581 gagccatacc aggcttccgc cgcggccgtc gtcgacttcg gcgacgaaat cgaccggctg
     2641 ggaggcgttg gcgatggtgc aggcgtgcac cgcagaggcg tggagatcgt tgcccgccga
     2701 ggccggaagg ctgaggcttg cgccgagcac caacgcggcg aggatcgcgg cgagggagaa
     2761 ataagcgaat ttcatgattt gcttcctttt gacgcctccc cggcaggatt gcctcgaggc
     2821 ttggcgcctt agatggagcg cgctctcgtc cccctttgtg agcctgctca caaagacctc
     2881 gcctggaagc gcgggctagg tctggtcctc cttcttctcg acatggccaa gctcgcgctc
     2941 cggcgcgatt tcgtcccgca cgatttgctt cagctccttg atctccggga agcgcccccg
     3001 ctccccgcgc gaccaaatca gcttgccgtc gatcttgatc tcgaacacgc cgccggtgtc
     3061 gtcaggaatg agcgtgaccg agcctagctc ctccgcgaag gtcgacagca attcctgctg
     3121 catccaggcg gccctgagca gccagttgca cagccggcaa taggtgatgg cgacgttggg
     3181 ggcgggcgga atggcgaaga tccagca
//
LOCUS       DATNAE010000239         4943 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2955259, whole genome shotgun sequence.
ACCESSION   DATNAE010000239 DATNAE010000000
VERSION     DATNAE010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4943
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2955259"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VN518_05430"
     CDS             complement(<1..191)
                     /locus_tag="VN518_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HXE04049.1"
                     /translation="MFVVVRMPDGSSHRLEALEGWRVMEVIRDWGLPIKAECGGACAC
                     ATCHVWLDEAWVAKLPAPT"
     gene            complement(193..1221)
                     /locus_tag="VN518_05435"
     CDS             complement(193..1221)
                     /locus_tag="VN518_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007197719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXE04050.1"
                     /translation="MAANGSTDVIIIGAGPVGLFAVFQLGLAKLKAHVVDVLDRPGGQ
                     CAELYPEKPIYDIPGLPVCTGQELTDRLLEQIGPFEPVFHFGEMAAALSKTEHGQWQL
                     TTDSGLIITAPVLVIAAGGGSFVPKRPPIPGIEGYEGKSVFYAVRRIEEFRGRHIVLA
                     GGGDSALDWTVNLAPIAGSLTLLHRRPEFRAAPATVSKMRELEAEGLVRFELGQPVGL
                     KGAGGKLEAVAVKRMDGSTGEIAADTLLPFFGLTMKLGPIANFGLNLNENLIPVDTEK
                     FETGTKGIFAIGDINTYPGKLKLILSGFHEAALMAQAAHRYVHPDKPLRFQYTTSSSE
                     LQRLLKVA"
     gene            complement(1448..1867)
                     /gene="mscL"
                     /locus_tag="VN518_05440"
     CDS             complement(1448..1867)
                     /gene="mscL"
                     /locus_tag="VN518_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HXE04051.1"
                     /translation="MLNEFKEFALRGNVMDMAVGIIIGAAFSTIVRSLVDDIIMPPIG
                     VVTGGVDFSSIFIALNGQHYYSLAAARQAGAPTMNIGLFINNVIAFVIVAFVLFMAIK
                     AMNQLRRKQEEEPASEPPPSREVELLTEIRDALVKRG"
     gene            2041..2301
                     /locus_tag="VN518_05445"
     CDS             2041..2301
                     /locus_tag="VN518_05445"
                     /inference="COORDINATES: protein motif:HMM:NF025305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/substrate symporter small subunit"
                     /protein_id="HXE04052.1"
                     /translation="MSEHPGRDRHRWKTLILAIATLIIVFAAVFAVVAFAGPLDQVGF
                     LRFPLGFYLLAQGLLIGMVALAFWSTRVQERIDRSFSEGEEI"
     gene            2309..4144
                     /locus_tag="VN518_05450"
     CDS             2309..4144
                     /locus_tag="VN518_05450"
                     /inference="COORDINATES: protein motif:HMM:TIGR03648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VC_2705 family sodium/solute symporter"
                     /protein_id="HXE04053.1"
                     /translation="MSPPRRRQANPHLGAYYGILTSAFVSLAISLAMFEQLGWQETWV
                     ARAMILVPLALYVVIALGARTLNVEDFFASGRRVPPVYNGAVLAAVLIGGTGFFAYTG
                     TLFFLGFDALAIGLAWTAGLIVSGILFVPYLRKAGAYTMPAFLGQRFRSRFVRAMASV
                     LQLPPASLLLAAEIKVAALIIAMFLPLSHQLAVACVAVTIACVTIVGGMRSLTWSASA
                     QFIVSAVGFAVPLIIVSVLLTNLPAPQLTYGEMFEPLKRSESVTGVSPSAPSEFATAL
                     PREQPEPSAKPFLQAFGAVSQTDFVTLFLCLTLGAAALPSLLARSGVTSSVADQRRSV
                     AWGVFFVALFAISAPALAAFVRLMMFQDIAQAPANSLPSWLNDLSAYRLLFPKDANSD
                     GAIEASELFVARDGVVLALPMAAKLPFVCTVLTAAAGLAIALAAAASHLFTLAGSLAD
                     DVIRLVDPRSEVLPRLMVAWVAIAATALAAGVFLAFAEIDVMQVAIMAFASAAAIFFP
                     VLLLAIWWRRCTWLGAMLALGLGFATMATAIAFGDAPIAGQFRLTTVIAALIGAALGL
                     IGGLIGSLLGPKPTPAEQAYFEELRDPSGDALYDRARRRAAAASQ"
     gene            4175..>4943
                     /gene="pip"
                     /locus_tag="VN518_05455"
     CDS             4175..>4943
                     /gene="pip"
                     /locus_tag="VN518_05455"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HXE04054.1"
                     /translation="MQDRYDMNERHIQAGERLSLFPEIVPYRTGRLKVSPLHELYFEE
                     CGNPDGKPAVLLHGGPGGGSTAVMRRYHDPSRYRIVQFDQRGCGRSTPHASIDENTTW
                     HLVDDIEQLREHLNIERWQVFGGSWGSTLALAYAETHPERATELVLRGIFTLRRAELE
                     WFYQEGCSWIFPEAFEDYLRPIPVPERGDLIAAYYLRLTDPDPDVQLKAARAWSAWEG
                     KTLSLLPDPERVRRFSEDAYALAFARIECHYFVHGGFF"
BASE COUNT          808 a         1627 c         1528 g          980 t
ORIGIN      
        1 tcggtggggg cgggcaattt cgctacccat gcctcgtcca accacacatg gcaggtggcg
       61 caggcgcagg cgccgccgca ttcggccttg atgggcaaac cccagtcgcg gatcacctcc
      121 atgacccgcc agccctccaa cgcctcgagg cgatgggagc ttccgtccgg catacgcacg
      181 acgacgaaca tgtcaggcca ccttcaacag acgctgcagc tcggacgagg aggttgtgta
      241 ctggaagcgc aaaggcttgt caggatggac gtagcgatga gcggcttgcg ccatgagcgc
      301 agcctcatga aaccctgaaa ggatgagctt tagcttaccg ggataggtat tgatgtcgcc
      361 gatggcgaaa atgcccttgg tgccggtctc gaatttctcg gtgtcaaccg ggatcaggtt
      421 ctcgttgaga ttgagcccga aattcgcgat cggcccgagc ttcatggtaa ggccgaagaa
      481 gggaagcagc gtgtcggcgg caatctctcc ggtcgagccg tccatgcgct tgaccgcgac
      541 ggcctcaagc ttgccgccag cgcctttcag cccaacaggc tgaccaagct cgaaacgcac
      601 cagcccctcc gcttccagct cgcgcatctt gctgacggtc gccggggcgg cgcggaactc
      661 aggcctgcga tgcaggagcg tgagcgagcc ggcgatcggg gcaagattga cggtccagtc
      721 gagcgctgaa tcgccacctc cggcgaggac gatgtggcgg ccgcggaact cctcgatgcg
      781 gcgcacggcg tagaacacgc tcttgccttc atacccttcg atgccgggga tgggcggacg
      841 cttcggcacg aagcttccgc cgccggcggc gattaccagg acgggcgcgg tgatgataag
      901 gccgctgtcg gtggtgagct gccactgtcc atgctcggtc ttgctgaggg cagcggccat
      961 ctcaccgaaa tggaacacag gctcgaacgg cccgatctgc tcgagcagcc ggtcggtcag
     1021 ctcttggccg gtgcaaaccg gaaggccggg aatgtcgtag atcggcttct cgggataaag
     1081 ctcggcgcat tggccgccag gccggtcgag aacatcgacc acgtgcgcct tgagcttggc
     1141 gaggccgagc tgaaatacgg cgaaaaggcc gactggcccc gccccaataa taatgacatc
     1201 ggtggagccg tttgccgcca tcgcctgctt gttcccgcct gaaggttgcc taacgcgcta
     1261 tgtaaggcag cttagtgaaa aatcattctg ccaggaagct atctgaggca aaagaacgca
     1321 aactcattct atttcctagg aaatgatgga atttttttca aacggcgcgc ggcgcgtaac
     1381 ggccatgccg cgaggggcgc caatcctgca aaatcattcc tgcctgaagg cttccgcgcg
     1441 gggcgagcta gccccgtttc accagggcgt cccgtatttc cgtcagcagc tcaacctcgc
     1501 gggacggcgg cggctcgctg gccggctctt cctcctgctt gcggcggagc tggttcatgg
     1561 ccttgatcgc catgaacagg acgaaggcga cgatgacgaa ggcgatcacg ttgttgataa
     1621 agaggccgat attcatggtc ggggcgccgg cctgccgcgc cgcggcgagt gagtagtagt
     1681 gctggccgtt gagcgcgatg aagatgcttg agaagtcgac gcccccggtc acgacgccga
     1741 tcggcggcat gatgatgtcg tcgaccagcg acctgacgat ggtggagaag gcggcgccga
     1801 tgataatgcc gaccgccatg tccatgacgt tgccgcgtag cgcaaattcc ttgaattcgt
     1861 tcagcatcgc cgggtgtcct cgaaaaagct cccattggct caagcctcta gcttgccatg
     1921 tttgggcgcc aatccaaacc cggctgtaac aaggcttgaa tttcgggcac ccccctttgg
     1981 caacaggtgc cgcaactggt aactgcttgg cggccacgcg cttcttcagg gagagcttcg
     2041 gtgtcggaac acccaggacg cgatcgtcac cgctggaaga cgctcatcct tgccatcgcc
     2101 acgctgatca tcgttttcgc cgcagtgttc gccgtggtgg ccttcgccgg accactcgat
     2161 caggtcggat tcttgcgctt tccgctcggc ttctacctgc tggcgcaggg ccttttgatc
     2221 ggcatggtgg cgctcgcctt ctggtcgacc cgcgtgcagg agcgcatcga ccggagcttc
     2281 agcgaaggcg aggagatttg agcgcgccat gagccccccg cgccgccgtc aagcgaaccc
     2341 ccatcttggc gcctattacg gcattctcac cagcgccttc gtttcgctcg cgatctcgct
     2401 cgccatgttc gagcagctcg gctggcagga gacgtgggtg gcgagagcca tgatcctcgt
     2461 gccgctcgcg ctctatgtcg tcattgcgct cggagcccgc acgctcaacg tcgaggattt
     2521 cttcgcttct gggcgccgcg tgcctcccgt ctataacggc gccgtgctcg ccgcggtgtt
     2581 gatcggcggc accggatttt tcgcctatac cggcacgctg ttctttctcg gcttcgacgc
     2641 gctcgccatc gggcttgcct ggacggcggg cctcatcgtg tcaggcatcc tgttcgtgcc
     2701 ttacttgcgc aaggccggcg cctacaccat gcctgccttt ctcggccagc gcttccgctc
     2761 gcgttttgtg cgcgccatgg cgagcgtgct gcaacttccg ccggcttcgt tgctgcttgc
     2821 cgccgagatc aaggtcgcgg ccctgatcat cgcgatgttc ctgccgctct cccatcagct
     2881 cgccgtcgct tgcgtggccg tgaccatcgc ctgcgtcacc atcgtcggag gcatgcgctc
     2941 gctcacctgg agcgcgagcg cgcaattcat cgtcagcgcc gtgggctttg ccgtcccctt
     3001 gatcatcgtc tcggtgctgc tcaccaatct tccggcgccg caactgacct atggcgagat
     3061 gttcgagccg ctgaagcgct cggagagcgt caccggcgtt tcgccttcag cgccaagcga
     3121 gtttgcgacg gcgcttcccc gcgagcaacc agagccgagc gccaagccct tcctgcaagc
     3181 cttcggcgcg gtcagccaaa ccgacttcgt gacgttgttt ctgtgcctca cgcttggcgc
     3241 cgcggcgctg ccgagcctgc ttgcccgaag cggcgtcacg agctcggtcg ccgatcagcg
     3301 gcgctcggtc gcctgggggg tgttcttcgt ggctctgttc gccatcagcg cgccggcgct
     3361 cgcagccttc gtcaggctaa tgatgttcca ggacattgcc caagcgccgg caaactcgct
     3421 tccctcctgg ctcaacgatt tgagcgcgta ccggctgctc ttccccaagg acgccaacag
     3481 cgatggcgcc atcgaggcgt cggagctttt cgtcgcgcgc gacggcgtcg tgctcgccct
     3541 gcccatggcg gcgaagttgc cgttcgtctg caccgtgctc acggctgcgg cgggcttggc
     3601 catcgcgctc gccgccgcgg catcacatct cttcaccctt gccggcagcc ttgccgacga
     3661 cgtcatcagg ctggtcgacc cgcgcagcga ggttcttccg agactgatgg tggcgtgggt
     3721 ggcgatcgcc gcaacggcgc tcgccgccgg cgtgtttctc gcctttgccg agatcgatgt
     3781 gatgcaggtc gccatcatgg cctttgcctc ggccgcggcg attttctttc cggtgctgct
     3841 tctcgccatc tggtggagac gctgcacttg gctcggcgcc atgcttgccc ttggcctcgg
     3901 cttcgccacc atggctaccg caatagcctt cggggatgcg cccatcgccg gtcagttccg
     3961 cctcaccacg gtgatcgccg cgctgatcgg cgctgcgctt gggctgattg ggggcctgat
     4021 cggcagcctg ctcggaccga agccgacgcc ggccgagcaa gcctatttcg aggaactgcg
     4081 cgatccgtca ggcgatgcgc tctacgatcg cgcacgacgg cgcgcggcgg cggcaagtca
     4141 atagcagtct tcactcccca acaagggcgc catcatgcaa gatcgctacg acatgaatga
     4201 gcgtcacatc caggcaggag agcgcttgag cctcttcccc gagatcgtcc cgtaccgcac
     4261 gggacggctc aaggtttctc ccttgcatga gctttacttc gaggaatgcg gcaatccaga
     4321 cggcaagcct gccgtgctgc tgcatggcgg ccccggcgga ggctccaccg ccgtgatgcg
     4381 ccgctatcac gatccgtcac gttaccgcat cgtgcagttc gaccagcgcg gctgcggccg
     4441 ctccacgccg catgccagca tcgacgagaa caccacctgg catctcgtcg acgacatcga
     4501 gcagcttcgc gaacatctca acatcgagcg ctggcaggtg ttcggcggct cctggggctc
     4561 gacgctcgcg ctcgcctatg ccgagacgca tccggagcgg gcgaccgagc tcgtgctccg
     4621 cggcatcttc acgctcaggc gcgccgagct cgaatggttc tatcaggagg gctgcagctg
     4681 gatcttcccc gaggcgttcg aggattatct gcgcccgatt ccggtgcctg agcgcggcga
     4741 tctgatcgca gcctattact tgcggctgac cgatcccgat ccagacgtgc agctcaaggc
     4801 tgcgcgtgcc tggagcgcgt gggaaggcaa gaccttatcg ctcctacccg atccggagcg
     4861 cgtgcgccgc ttctccgagg acgcctatgc cctcgctttc gcgcgcatcg agtgccacta
     4921 cttcgttcat ggcggcttct tcg
//
LOCUS       DATNAE010000240         2683 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2049769, whole genome shotgun sequence.
ACCESSION   DATNAE010000240 DATNAE010000000
VERSION     DATNAE010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2049769"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="VN518_05460"
     CDS             <1..949
                     /locus_tag="VN518_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HXE04055.1"
                     /translation="VGLFVIDILAMLALSGGVRFRRRAAATGAILLALALLPVASHRA
                     EAQDAPVTAPPTAAEAGATTSPASPADFALKASLQTHLAYVITGDPEIDRISEEGLSG
                     LSKVLRARTALEPADPMGVDIDKDELAFFPILYWPVREDVQPLSDATLAKVDAFMKQG
                     GLIVFDTRDHETGFSGTGAQTKALTRLIGQLDIPPLEAVPENHVLTKSFYLMHSFPGR
                     WDGGALWVEAEPEDETERNERSRRTDGVSSIVITSNDFAGAWALDDANRPIYPAVPGG
                     EVQREMAFRAGVNLVMYALTGNYKADQVHVPALLERLGQ"
     gene            954..>2683
                     /locus_tag="VN518_05465"
     CDS             954..>2683
                     /locus_tag="VN518_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE04056.1"
                     /translation="MSWSIEFLPFVPWPVLWALAGLGAVLLALLFWRSRRGAIFRLLS
                     YALLLLAIANPQLKQEDREPLNDVLAVVVDVSQSQAIAGRTARTAELRKELEERLKSF
                     PNLEVRWVNSNSTSADSERDGTMLFTDLAQALADVPPDRLAGVIMITDGEVHDVPASA
                     ASLGFEAPVHALLTGKPGEFDRRLQVLTAPRFGIVGSSQNIEVKVTDSTASGAGGPVK
                     LTVTHQGQPPVMQSVQLGEKVEIPVGIDHAGPNIIEIEAEPAKGELTTVNNRAVLTVE
                     GVRENLRVLLVSGEPHAGERTWRNMLKSDASVDLVHFTILRPPEKQDGTPINQLSLIA
                     FPTRELFQEKLDQFDLIIFDRYQRRGVLPLLYLENVARYVEKGGAVLVSAGDDYASPL
                     SLYRSPLGEILPAVPSGRVIEEPFKPRLTELGEKHPVTSDLPGAAGPDPTWGHWFRVV
                     DVSPRDGEVLMKGAQDKPLLIIGQRGEGRVGMLLSDHAWLWARGYEGGGPYTDLLRRL
                     AHWLMKEPDLEEETLKATSRGQTLSIERRSVTDKVDPVTVTAPSGKEETVTLDKGERG
                     LWHKAMTVSE"
BASE COUNT          446 a          906 c          878 g          453 t
ORIGIN      
        1 cgtcggactg ttcgtcatcg atatccttgc catgctggca ttgagcggcg gggtgcggtt
       61 ccgccgccgc gccgccgcta ccggcgccat cttgctcgcg ctggcgctgc tgcccgtagc
      121 cagccatcgt gccgaggcgc aggacgctcc ggtaacggct ccgccgacag cagcggaagc
      181 cggggccacc acttcgcccg cctctcccgc cgatttcgcc ctgaaggcat cgctgcagac
      241 ccatctcgct tacgtcatca ccggcgatcc cgaaatcgat cgcatcagcg aggaaggctt
      301 gagcgggctg agcaaggtgt tgcgcgcgcg caccgcgctc gagcctgccg atcccatggg
      361 cgtcgatatc gataaggacg agcttgcctt ctttccgatc ctgtattggc ccgtgcgcga
      421 ggatgtgcag cctctctcgg acgcgaccct cgccaaggtg gatgccttca tgaagcaagg
      481 cggcctgatc gtgttcgata cgcgcgacca cgagaccgga ttctccggca ccggcgcgca
      541 gaccaaggcg ctcacccgcc tgatcggcca gctcgacatt ccaccgctgg aggcggtgcc
      601 cgagaaccac gtgctcacca aatcgttcta tttgatgcac agcttccccg gccgctggga
      661 cggcggcgcg ctgtgggtcg aggccgagcc ggaggacgag accgagcgca acgagcgctc
      721 gcgccgcacc gacggcgtga gctcgatcgt catcacttcc aacgactttg ccggggcctg
      781 ggcgctcgac gacgccaacc ggccgatcta cccggccgtg ccgggcggcg aggtgcagcg
      841 cgaaatggcg ttccgcgccg gcgtcaacct ggtgatgtac gcgctcaccg gcaactacaa
      901 ggccgatcag gtgcacgtcc ctgcgctgct cgagcggttg gggcagtgac gtcatgagct
      961 ggtcgatcga attccttccc ttcgtgccct ggccggtgtt gtgggcgctc gccggcttgg
     1021 gcgcggtgct gctcgcgctg ctgttttggc gctcgcggcg cggcgctatc ttccggctgt
     1081 tgagctacgc gctgctgttg ctcgctatcg ccaatccgca attgaagcag gaggaccgcg
     1141 agccgctcaa cgacgtgctt gccgtggtgg tcgacgtcag ccaaagccag gccatcgccg
     1201 gccgcaccgc gcgcacggcc gagttgcgca aggaattgga ggagcggctc aagtccttcc
     1261 ccaacctcga ggtgcgctgg gtcaattcga actcgacctc ggcggatagc gagcgtgacg
     1321 gcaccatgct gttcaccgac ctcgcgcaag ctcttgccga cgtgccgcca gaccggcttg
     1381 ccggcgtgat catgattacc gacggcgagg tgcacgacgt gccggcttcg gccgcgagcc
     1441 tcggtttcga ggcgccggtg catgccttgc tcaccggcaa gcccggcgag ttcgaccgca
     1501 ggctccaggt gttgaccgcg ccacggttcg gtatcgtcgg ctcgagtcag aacatcgagg
     1561 tcaaggttac cgacagcacc gcttccggcg ccggaggccc ggtcaagctg accgtgaccc
     1621 atcaaggcca gccgcccgtc atgcagagcg tgcagctcgg cgagaaagtt gagatcccgg
     1681 tcggtatcga ccatgccggc cccaacatca tcgagatcga ggccgagccg gccaagggcg
     1741 agctcaccac ggtcaacaat cgcgccgtgc tgacagtgga gggcgtgcgg gagaatctgc
     1801 gcgtgctgct ggtctcgggc gaaccgcatg ccggcgagcg cacctggcgc aacatgctga
     1861 aatccgacgc ttcggtcgac ctcgtgcatt tcaccatcct gcgtccgccg gagaagcagg
     1921 acggcacgcc gatcaatcag ctctctctga tcgccttccc gacgcgcgag ctgttccagg
     1981 agaagctgga ccagttcgac ctgatcatct tcgaccgcta ccagcggcgc ggcgtgctgc
     2041 ctctgctcta tctggaaaat gttgcacgct atgtggagaa gggcggcgca gtgctcgtct
     2101 cggcgggcga cgattacgcc tcgccgctca gcctctatcg ctcgccgctc ggggagatcc
     2161 tcccggctgt gccctcaggg cgcgtgatcg aggagccatt caagccgcgc ctcaccgagc
     2221 ttggcgagaa gcatccagtg accagcgatc tgccgggcgc cgcgggtcca gatcccactt
     2281 ggggacattg gttccgcgtg gtcgatgtca gcccgcgtga cggcgaggtg ctgatgaagg
     2341 gcgcgcaaga caagccgctc ttgatcatcg gccagcgcgg cgagggccgc gtcggcatgc
     2401 tgctctccga ccatgcctgg ctctgggcgc gcggttatga gggcggcgga ccgtacaccg
     2461 atctcctgcg ccggctcgcc cattggctga tgaaggagcc cgacctcgag gaggaaacgc
     2521 tgaaagcgac cagccgcggc cagacgctct ccatcgagcg ccgctcggtg acggacaagg
     2581 tcgatccggt caccgtaacc gcgccctcgg gcaaggaaga gaccgtgacc ctcgacaagg
     2641 gcgagcgcgg cctgtggcac aaagccatga ccgtgagcga gca
//
LOCUS       DATNAE010000241         2167 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3847494, whole genome shotgun sequence.
ACCESSION   DATNAE010000241 DATNAE010000000
VERSION     DATNAE010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3847494"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..1352
                     /locus_tag="VN518_05470"
     CDS             3..1352
                     /locus_tag="VN518_05470"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HXE04057.1"
                     /translation="MQQVPEYPDLRALDDVEKQLATFPPLVFAGEARKLKRALGKAAA
                     GEAFLLQGGDCAESFAEHRADSIRDFFRVFLQMAVVLTYAGGSPVVKLGRIAGQFAKP
                     RSAPMETQDGKELPSYRGDIINGIDFTEAARIADPNRMLMAYRQSAATLNLLRAFAQG
                     GYANLEHVHRWNLGFVKDSPAGHRYEEVAEHIAETLRFMRAIGLTPDNAPQLRTTSFY
                     TSHEALLLGYEQAFTRVDSTSGDWYATSGHFLWAGDRTRQLDHAHIEYLRGIKNPIGA
                     KIGPSLQPEALLTLIDVLNPQNEPGRLTLISRFGADKVGKHLPALIRAVKREGKHVVW
                     ACDPMHGNTIKAGSGYKTRPFDLILSEVETFFDVHRSEGTHAGGIHVEMTGQNVTECV
                     GGAKAVTETDLHDRYHTHCDPRLNADQSLELAFIVAERLKREREGRPEPEISIAAGE"
     gene            1471..>2167
                     /locus_tag="VN518_05475"
     CDS             1471..>2167
                     /locus_tag="VN518_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HXE04058.1"
                     /translation="MAHRIKDSFKLALAQLDAVVGDIDGNLRRAREARARGAQAGADL
                     IAFTELFLTGYPIEDLVLKPALQKAAQAALESLARDTKDDGPAVLMGLPFADGPFVYN
                     AQALLDRGRIEALRFKNNLPNYGVFDEKRVFAPGPMPAPIEFRGIKLGIPVCEDIWSE
                     QVCKALAVGGAELLIVPNGSPYWIDKQDVRYGVAEARVKETGLPLAYVNQVGGQDELV
                     FDGASFVLNEDASL"
BASE COUNT          388 a          698 c          716 g          365 t
ORIGIN      
        1 cgatccagca ggttcccgag tacccggacc tgcgggccct cgacgacgtc gagaagcagc
       61 tcgcgacctt tccgccgctg gttttcgccg gcgaggcgcg caagctgaaa cgggcgctgg
      121 gcaaggcggc ggccggcgag gctttcctgc tgcaaggcgg cgattgcgcc gagagcttcg
      181 ccgagcatcg cgccgattcc atccgcgatt tcttccgcgt gttcctgcag atggcggtgg
      241 tgctgaccta tgcgggcggc tcgccggtgg tgaagctcgg ccgcatcgcc ggccagttcg
      301 ccaagccgcg ctcggcgccg atggagacgc aagacggcaa ggagctgcca agctatcgcg
      361 gcgacatcat caacggcatc gatttcaccg aggccgcgcg gattgccgac cccaatcgca
      421 tgctgatggc ctatcggcag tcggcggcga cgctcaacct gctgcgcgcc ttcgcgcaag
      481 gcggctacgc caatctcgag cacgtgcatc ggtggaatct cggcttcgtt aaggacagcc
      541 ccgccgggca ccgctacgag gaagtcgccg agcacatcgc cgagaccttg cgcttcatgc
      601 gcgccatcgg gctcacgccc gacaatgcgc cgcagctccg caccacgagc ttctatacca
      661 gccacgaggc gctcttgctc ggctacgagc aggccttcac gcgcgtcgat tccacgtcag
      721 gcgactggta cgccacttcg ggccatttcc tctgggccgg cgaccgcacg cgccagctcg
      781 accacgcgca tatcgaatat ctgcgcggca tcaagaatcc gatcggcgcc aagatcgggc
      841 cgagccttca gcccgaggcg ctgctaacgc tgatcgacgt gctcaacccg cagaacgagc
      901 ccgggcggct cacgctgatc tcccgcttcg gcgccgacaa ggtcggcaag catttgccgg
      961 cgctgatccg cgcggtgaag cgcgagggca agcatgtggt atgggcgtgc gatcccatgc
     1021 acggcaacac catcaaggcg ggaagcggct acaagacgcg gccattcgat ctcatcctgt
     1081 ccgaggtcga gaccttcttc gacgtccacc gcagcgaggg cacgcatgcc ggcggcatcc
     1141 atgtcgagat gaccgggcag aacgtcaccg aatgcgtcgg cggcgccaag gccgtcaccg
     1201 agaccgatct gcacgaccgc taccacaccc attgcgatcc gaggctcaac gccgaccagt
     1261 cgctggagct tgccttcatc gtggccgagc ggctgaagcg ggagcgggaa gggcggcccg
     1321 agcccgagat ttccattgcc gcgggcgaat agaatctttg acttcggctg tcatgcccgc
     1381 gaaagcgggc atccagtagt cgctggagtc ggttattagt tggcttcgcg ggttactgga
     1441 ttgcccggtc aagccgggca atgacaaagc atggcgcatc ggatcaagga ctccttcaag
     1501 ctcgctctcg cgcagctcga cgccgttgtc ggcgatatcg acggcaatct gcggcgagcg
     1561 cgcgaggcgc gggcgcgcgg ggcgcaagcc ggcgccgacc tgatcgcttt taccgagctg
     1621 tttctcaccg gctacccgat cgaggacctg gtgctgaagc cggcgttgca gaaagcggcg
     1681 caggcggcgc tggaatcgct ggcgcgcgat accaaggatg acgggccggc cgtgctgatg
     1741 ggcctgccct tcgccgatgg accgttcgtc tacaacgccc aagctttgtt ggatcgaggc
     1801 cgcatcgaag cgcttcgctt caagaacaac ctgccaaatt acggggtgtt cgacgagaag
     1861 cgggtgttcg ctccggggcc gatgccggcg ccgatcgagt ttcgcgggat caagctcggc
     1921 attccggtct gcgaggatat ctggagcgag caagtgtgca aggcgctggc tgtcggaggg
     1981 gcggagctac tgatcgtgcc caacggatcg ccctattgga tcgacaagca ggatgtgcgt
     2041 tacggcgttg ccgaggcgcg cgtgaaggaa acggggctac cgctcgctta cgtcaatcag
     2101 gtgggcggac aggacgagct cgtgttcgac ggcgcctcct tcgtgctgaa cgaggatgcg
     2161 agccttg
//
LOCUS       DATNAE010000242         5475 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_244113, whole genome shotgun sequence.
ACCESSION   DATNAE010000242 DATNAE010000000
VERSION     DATNAE010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5475
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244113"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VN518_05480"
     CDS             <1..372
                     /locus_tag="VN518_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE04059.1"
                     /translation="QGAVARQLAAIEAIRAKVQSCSNFSTLTKDIKGFKVKALQDQLP
                     GSLGQPMRTLVMNAKVGQMTPPTLSGSAIEAYAVCGKHAVKGDPEKRQQTEMKLLEQE
                     LGIRAEGLLRDMRQDAFIEYR"
     gene            501..1517
                     /gene="pdxA"
                     /locus_tag="VN518_05485"
     CDS             501..1517
                     /gene="pdxA"
                     /locus_tag="VN518_05485"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007196827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HXE04060.1"
                     /translation="MESAERPLALTLGDPAGIGPDITLLAYAARRSAPVPPFVLIGDP
                     SVLAERARALGLDIEIATVGDAAEAPSLFAKTLPVLPVSVAGDVVAGRPDSAAAGAVQ
                     RSIERAVELVRAGTASAVVTNPISKAILIHAGFGFPGHTEFLARLAGLDASDVVMMLA
                     APELKVVPVTIHIPLKEVVAALDEGRIVRTLEITARDLTRYFGVMRPRIAVTGLNPHA
                     GEDGTMGREEIEMIMPAIAAARRQGFDVTGPYSADTLFHAAARKTYDVAVAMYHDQAL
                     IPIKTLAFDLGVNVTLGLPFVRTSPDHGTAFALAGTGQASPRSLIEALRLANAMQARA
                     DAKP"
     gene            1514..2356
                     /gene="rsmA"
                     /locus_tag="VN518_05490"
     CDS             1514..2356
                     /gene="rsmA"
                     /locus_tag="VN518_05490"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018261034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HXE04061.1"
                     /translation="MSTPDGLPPLRDIIRASGLSARKSLGQNFLLDFNLTRRIARAAG
                     PLEGTTVVEIGPGPGGLTRALLLEGASRVIAIEKDERCLPALSDIADVYPGRLDIVAA
                     DAREVDYGALGLRAPTRIVANLPYSVATPLLIGWLKTEPWPPWFDRLVLMFQREVALR
                     IVAKPGGKDYGRLAVLTQWRTTPRILLTLPAAAFTPRPKVDSSLVEIVPKEAPLPACD
                     VVALERVTAAAFGQRRKMLRSSLRQLTPDSEAMLQELGIDPKARAEELEIADFCRIAN
                     ALQR"
     gene            complement(2353..2982)
                     /gene="gmk"
                     /locus_tag="VN518_05495"
     CDS             complement(2353..2982)
                     /gene="gmk"
                     /locus_tag="VN518_05495"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HXE04062.1"
                     /translation="MEPTRRGLMLVLSSPSGAGKTTLARRLLEADPGVVPSISCTTRK
                     QRKGEVEGRDYYFVDRDTFARMRDRGEFLEWALVFDNFYGTPREPVERALAEGRDVLF
                     DVDWQGAESLRDEKKGDVVSVFILPPSGEALERRLNERAQDSPDVVEARMRGASNEIQ
                     HWHHYDYVVINHDLDQALAALRAILAAERLRASRLTGLKDFVQNLLAEL"
     gene            complement(2991..3878)
                     /locus_tag="VN518_05500"
     CDS             complement(2991..3878)
                     /locus_tag="VN518_05500"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019966094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HXE04063.1"
                     /translation="MTLKSMTGFGRSSGVHGETSWHWEVRSVNGRGLELRLRMPSGLE
                     RLEIKARSLAQEALARGNCTLNLTLRRETGELAIRLNEAALVQALAAAERAQALTHKE
                     APNLDTLLAMRGVIEVVEGEESEEAHAKLEAALLKGLGTALDQLVAGRTAEGARLAGV
                     MTNQLDQIESLVERATSAMGHRPEAIQQRLTEQVKRLTETGAGLDPERLHQEALLLAA
                     KSDIQEELDRLRSHVAAAKELLAQDEPAGRRLEFLAQEFNREANTLCSKASDIEISRA
                     GLELKSVIDQLREQVQNIE"
     gene            complement(4010..5455)
                     /gene="mltG"
                     /locus_tag="VN518_05505"
     CDS             complement(4010..5455)
                     /gene="mltG"
                     /locus_tag="VN518_05505"
                     /inference="COORDINATES: protein motif:HMM:NF014656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HXE04064.1"
                     /translation="MSVTSDSSSTASDNDACERAERAMRNFDTPPRSPYEALEPVRPP
                     ECPEREPWRMHPVLAFFNALFTLTFFTVCVVFVLFYFFRVQFDRPGPLPTSTVVVIPK
                     GEGVSGIAERLERDEVITDRGLFMTSIIYFMYLRGTGALKAGEYEFRKNASMRQVLDT
                     LVEGKSIAHKVTLAEGLTSQQIVERIDANPDLKGSIAAVPAEGTLLPDTYRFQIGDSR
                     QDIIERMQVAQKKFLAKMWEERDPDIIVQTPEEAIILASIVEKETSRADERPLIASVY
                     QNRLRKKMRLQSDPTIIYGLVGGKGVLDHPIQQEELDRDTAYNTYKINGLPPGPIANP
                     GRAAIEAVLKPAKTKDLYFVADGSGGHAFAASLDEHNKNVVKWRQVERALREQERAEA
                     AAAAAAGTPSPDGGIPKMSPGAPAPGTGTATGAAPGTLVAPDALVNLRSSAGISPAPA
                     APAGETGAEPEAGPDADTGAGIPLPRRNPLR"
BASE COUNT          890 a         1868 c         1740 g          977 t
ORIGIN      
        1 caaggggcgg tcgccaggca gcttgccgcg atcgaagcca ttcgtgccaa ggtgcagtct
       61 tgctccaact tctcgacgct caccaaggac atcaagggct tcaaggtgaa agcgctgcag
      121 gatcagctgc cgggctcgct cggccagccg atgcgcacgc tggtgatgaa cgcgaaggtg
      181 ggtcaaatga ctccccccac cctctcaggc tcggccatcg aggcctacgc ggtttgcggc
      241 aagcatgccg tcaagggcga tccggagaag cggcagcaga ccgagatgaa gctcttggag
      301 caggagctcg ggatcagggc cgaaggcctg ctgcgcgaca tgcgccagga cgcgttcatc
      361 gagtaccgtt gacgcggttt cgtgtgacgc gggcgccgga tcggcttaaa gactttcgcc
      421 aatcacttaa gtccgggcgt gctgcccctg agcggcagct gacaaacgct gcggagcgtc
      481 ttgccgatgg ccgggtcgca atggagagtg ccgaaagacc gcttgccctc accctcggcg
      541 atcccgccgg gattggcccc gacataaccc ttcttgccta tgcggcgcgt cggagcgcgc
      601 cggttccgcc cttcgtgctg atcggcgacc ccagcgtgct cgctgagcgg gcccgcgcgc
      661 tcggtctcga catcgagata gcgaccgtcg gcgacgcggc cgaggcaccc tctctctttg
      721 ccaagacctt gccggtgctg cccgtctccg tcgccggcga tgtggttgcc ggacgccccg
      781 actctgccgc agcgggcgcc gttcagcggt cgatcgagcg ggccgtcgag ctcgtccgcg
      841 ccggcacggc aagcgccgtg gtcaccaacc cgatcagcaa agcgatcctg atccatgcgg
      901 gcttcggctt ccccggccac accgagtttc tcgccaggct tgccgggctc gacgcctccg
      961 acgtggtgat gatgctcgca gcacctgagc tgaaagtggt tccggtcacc atccatattc
     1021 cgctcaaaga ggtggtggct gcgctcgacg aggggcgcat cgtcaggact ctcgagatca
     1081 cggcgcgcga ccttacgcgc tatttcggcg taatgcggcc gcgcatcgcc gtcaccggcc
     1141 tcaaccccca tgccggagaa gacggcacca tggggcgcga ggagatcgaa atgatcatgc
     1201 ccgccatcgc tgccgcccgc cgtcaaggct tcgacgtgac cgggccctat tccgccgaca
     1261 ccctgttcca cgccgcggcg cgcaagacct acgacgtggc tgtagccatg tatcacgacc
     1321 aggccctcat cccgatcaag acccttgcct tcgacctcgg cgtcaacgtc acccttgggc
     1381 tgcctttcgt gcgcacctcg cctgaccacg gtacggcgtt tgcgcttgcc ggcaccggtc
     1441 aagcgagccc gcgtagcctg atcgaggcct tgcggctcgc caatgccatg caggcccgcg
     1501 cggacgccaa gccttgagca cgccagacgg cctgccgccg ctccgcgaca tcatccgcgc
     1561 ctctgggctt agcgcgcgca agagcctcgg ccagaatttc ctgctcgatt tcaacctcac
     1621 ccgccgcatc gcccgcgccg ccggaccgct cgagggaacg accgtcgtcg agattggccc
     1681 agggcccggt ggcctcacgc gggcgctgct tctcgaaggt gcgtcgcggg tgatcgccat
     1741 cgaaaaggat gagcgctgcc tccccgcctt aagcgacatc gccgatgtct atcccggacg
     1801 tctcgacatc gtcgccgccg atgcgcgcga ggtcgactac ggcgccttgg gcttacgggc
     1861 gcccacgcgc atcgtcgcca acctgcccta cagcgtcgcc acgccgctcc tcatcggctg
     1921 gctcaagacc gagccatggc cgccttggtt cgatcggctc gtgctcatgt ttcagcgcga
     1981 ggtagcgctc cgcatcgtgg ccaagccggg cggcaaggac tacgggcgtc tcgccgtgct
     2041 cacccaatgg cgaaccaccc ctcgcattct gctcaccctt ccggccgccg ccttcacgcc
     2101 acgccccaag gtcgactcct ctctggtcga gatcgtgccg aaggaggctc cgcttcctgc
     2161 ctgtgacgtc gttgcattgg agcgggtgac ggcggcggcc ttcggccagc ggcgcaagat
     2221 gctgcgctct tccttgcggc agctcacccc cgacagcgaa gccatgctgc aagagctcgg
     2281 catcgatcca aaggcgcggg ccgaggaact agagatcgcc gatttttgcc ggatcgccaa
     2341 cgcgcttcaa cgctagagct cggccaagag gttctgcacg aagtccttga gcccggtgag
     2401 gcgcgaggca cgcaagcgct cggcggcgag gatggcgcgg agcgccgcga gggcttggtc
     2461 gagatcgtgg ttgatgacga catagtcgta gtgatgccaa tgctggatct cgttgctcgc
     2521 cccgcgcatg cgcgcctcga ccacatccgg cgaatcctgg gcgcgctcat tgaggcgccg
     2581 ctcgagcgcc tctcccgagg ggggcagaat gaacaccgag accacgtcgc ccttcttctc
     2641 gtcgcgcaaa ctctctgccc cctgccaatc gacgtcgaac aagacgtcgc ggccttcagc
     2701 caaagcacgc tcgaccggct ctcgcggcgt gccatagaaa ttgtcgaaga cgagggccca
     2761 ttccagaaac tcgccgcgat cccgcatgcg ggcaaaggtg tcacgatcta cgaagtaata
     2821 gtccctcccc tcgacctcgc ccttgcgctg cttccgcgtc gtgcaggaaa tcgacggcac
     2881 cacgcctgga tccgcctcga gcaatcggcg ggcgagcgtc gtcttccccg cccctgaggg
     2941 cgaggacagc accagcatca gacctcgtcg cgtgggctcc atcgctcgcc tcactcgatg
     3001 ttctgaacct gctcgcgcaa ctgatcgatc accgacttga gctcgagccc tgcccggctg
     3061 atctctatgt cggaggcctt cgagcagagc gtgttggcct cgcggttaaa ttcctgcgcc
     3121 aggaactcga gcctgcggcc ggccggctca tcttgcgcga gaagctcctt tgccgccgcc
     3181 acatgcgagc gaaggcggtc gagctcttcc tggatgtccg acttggcggc gagcaacagg
     3241 gcctcctggt gcagccgttc gggatcgaga ccggcgccgg tctcggtgag gcgcttcacc
     3301 tgctcggtca agcgctgctg aatcgcttca ggccggtggc ccatagcact cgtcgcgcgc
     3361 tcgactaggc tttcgatctg gtcgagctga ttggtcatga ccccggcaag cctcgcgcct
     3421 tccgccgttc gccccgcgac gagctgatcg agcgcggtgc caagcccctt gagcagcgcg
     3481 gcctcgagct ttgcatgcgc ctcctcgctc tcctcgccct ccaccacctc gatcacgccg
     3541 cgcatggcaa gcagcgtatc gagattcggt gcctctttgt gggtgagcgc ctgggcgcgc
     3601 tcggccgcgg cgagcgcctg gaccagcgca gcctcgttca ggcgaatggc gagctctccg
     3661 gtctcccggc ggagtgtgag attgagggtg caattgccgc gagctagcgc ttcctgggcg
     3721 agcgagcgcg ccttgatctc gagccgttca agacccgatg gcatgcggag gcgaagctca
     3781 agcccacgcc cattgacgct ccgcacctcc caatgccagc tcgtctcgcc atggacgcct
     3841 gagctgcggc caaatccggt catgcttttc agcgtcatgc gcgttcaacc tcatggccgg
     3901 ccgcagctca gcggacacga cccttatatg acgagtgggc cggattcggc gagcggaagg
     3961 ggggcacctc ggcgacaacg ttaacaggag aaggaggggc gcaaaggcct tagcgcaggg
     4021 gattgcgccg cggcaacggt atcccggcgc ctgtgtcagc gtccggccca gcctcaggct
     4081 cggccccggt ctcgccggct ggagctgcgg gcgcaggcga aatcccggcc gaagagcgca
     4141 aattcacgag agcatcgggc gcgacaagcg tgcctggtgc ggctccggtg gcggtgcctg
     4201 tgcctggagc tggggcgccc ggcgacattt tgggaatgcc accgtctggc gaaggcgtgc
     4261 cggcggcagc agcggcggca gcttccgcgc gctcctgctc gcgcagggcc cgctcgacct
     4321 gccgccactt caccacattc ttattatgct catcgaggct cgcagcgaag gcgtgccctc
     4381 cgctgccgtc ggcgacaaaa tagagatcct tggtcttggc cggcttcagc accgcctcga
     4441 tcgccgcccg ccccggattg gcgatcggcc ctggcggcag cccgttgatc ttataggtgt
     4501 tgtacgcggt gtcgcgatcg agctcttcct gctgaatggg atggtcgagc acgcctttgc
     4561 ctcccaccag cccgtagatg atggtggggt ctgattgcag ccgcatcttc ttcctgagcc
     4621 tgttctgata gacgctggcg atgagcggcc gctcgtcggc ccggctcgtc tccttctcga
     4681 cgatggaggc gaggatgatc gcctcctcag gggtttgcac aataatgtcg gggtcgcgtt
     4741 cctcccacat tttggcgagg aatttcttct gagcgacctg catccgctcg atgatgtcct
     4801 ggcggctgtc gccgatctgg aaccggtagg tgtcgggcag gagcgtgccc tcggcgggga
     4861 ccgcggcgat ggagcccttc agatctgggt tggcgtcgat gcgctcgacg atctgctggc
     4921 tggtgagccc ctcggccaaa gtgaccttgt gcgcgatcga cttgccctcg accagggtgt
     4981 cgagcacctg gcgcatgctg gcatttttgc ggaactcgta ctcgcctgcc ttgagcgctc
     5041 cggtgccgcg cagatacata aagtagatga tgctggtcat gaacaggccg cggtcggtga
     5101 tgacctcgtc gcgctcgagg cgctcggcga tgccgctcac cccctcgccc ttcggaatca
     5161 ccaccacagt ggaagtaggc aaggggcccg gccggtcgaa ctgaaccctg aagaaataga
     5221 acagcacgaa aacgacgcag accgtaaaga aggtgagcgt gaacagcgcg ttgaagaatg
     5281 cgagcaccgg gtgcatgcgc cagggctcgc gctccgggca ctccggcggg cgtacgggct
     5341 cgagcgcttc gtaggggctg cgtggcggcg tgtcgaaatt ccgcatcgcg cgttccgcac
     5401 gctcgcacgc atcgttgtcg ctcgccgtcg acgaggagtc gctggtcacg gacattgcct
     5461 taatgccccg cgcgg
//
LOCUS       DATNAE010000243         8366 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_1439160, whole genome shotgun sequence.
ACCESSION   DATNAE010000243 DATNAE010000000
VERSION     DATNAE010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 8366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8366
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1439160"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="VN518_05510"
     CDS             complement(<1..459)
                     /locus_tag="VN518_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HXE04065.1"
                     /translation="MKAFSVLLDRLAYTPSRNDKLRLLADYFASTPDPDRGWALAALT
                     DGLFFRLPLRRILGALIEARVDPVLFGLSRDYVGDTAETIALIWPGRDASEPAPRLDD
                     VVQTLKRAMPTEVPGLLSSWLDGLDATERWALLKLLTGALRVGVSARLAKT"
     gene            complement(456..1469)
                     /locus_tag="VN518_05515"
     CDS             complement(456..1469)
                     /locus_tag="VN518_05515"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     exonuclease"
                     /protein_id="HXE04066.1"
                     /translation="MHPERFESWLNVGTHGLYCRPGGFHIDPHSAVNRAVITHAHSDH
                     ARAGHDHVLATRETLALMKARLGSAAKLGTQALGYGETLQVGDVTVRLVPAGHVLGSA
                     QVVIEWRGRRAVISGDYKRSADPTNPPFELVRCDLFVTEATFALPVFTHGDAKGEVAK
                     LLKSLKQFPERAHVVGVYGLGKCQRLIALLREAGYAEPIYLHGALAACCQIYESFGVE
                     LGALKPATGAKREELKGAVVLAPPSAIADRWSRRMPEALTAFASGWMRVRARARQLGV
                     ELPLVISDHADWRELTRTIHETGAHEVWVTHGREDALLHHIQSTGRHGRALALIGRED
                     EES"
     gene            1577..2351
                     /gene="bcsS"
                     /locus_tag="VN518_05520"
                     /pseudo
     CDS             1577..2351
                     /gene="bcsS"
                     /locus_tag="VN518_05520"
                     /inference="COORDINATES: protein motif:HMM:NF028346.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsS"
     gene            complement(2870..4249)
                     /locus_tag="VN518_05525"
     CDS             complement(2870..4249)
                     /locus_tag="VN518_05525"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HXE04067.1"
                     /translation="MKSVMPANCRLYAVALAAAWLALASPGASAQDAPATESKPGGAT
                     LSIATWGGAYGQSQEIAYFQPFTQKTGVKIKTETYDGTLAAIKDKIGASSSPFDIVDL
                     SQGALDALCHGGLLEQIDSSTLTAGPGGQSVSDDFLSGGITSCGIASVAWSTAIAFDH
                     QAFTKAQPGKIADLLDIQRFPGKRALPNGPRYTLELALLADGVDPADIYTQLATPEGV
                     DRAFKALDKIKAQILWWDKAEDSISMLMRRKASMAAGYSGRIFRAAVGARQRIDVMWD
                     GQVYDLDLWAIPKAATNKDEAKRFIAFATEPAQMAAQASLIAYGPMRKSAIALVGKHP
                     SLDIDMKGYLPTAPDNFRKALRFDEAWWNEHGGELATRFQTWRDQPVVTDAKAAPPEG
                     KPSAADAKSKATETKPSTAEEKSKQGDAKAATADEKPKPAEAKPSTPEAQTGSVPSVA
                     PKPAPVQRR"
     gene            complement(4279..6111)
                     /locus_tag="VN518_05530"
     CDS             complement(4279..6111)
                     /locus_tag="VN518_05530"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HXE04068.1"
                     /translation="MKPSQLPWKWLLVGLLAVLVFAIALIPWLIGDTSRFGDRVASEL
                     SAWTGGEVKFIGPVRVRFLPDVSVRGQLEVQDSTKLPAVQTLTVREAKVSLDLVDLLR
                     GRITIDALRLLKPRVTLRDTLLAGASAATPAQDLFTSLLGGAPVRVLHVRNGRIALPA
                     DRSGAIKDIYAHIDAGKDTGAVSGFGSFAYKDTTVRYSLESGSPTASGGVESFPLALM
                     LSSKPLRVRLNGTASYAGEFKLDGDMQAEIDDGRKFLNWLGVRLPPGDSLKGFSASGG
                     FHLAGPALTFDDGTFTLDGNKAVGLLALSARGLRPRVEGTLAFDRLTLDPYLGKASSN
                     NGGAPPSTESTASFPFNRPLLQFIDADLRISAAAIDAGALKLGRGGFTVSAKQGVVAS
                     EVGELELCGGSADGRLDADLARPSKQVNLVANLDDVAIDACLQPLDLAAPISGAANIK
                     TELAAEGSDSTQFTGSLAGTLKLKAKDGVVPVDFARLLAGPTPLNDDGWSHDNATPFD
                     QLDADCRLGTGHIWCQSLSMRTPQGTIAGAGDVDVTKRTLDWSLTVANPIGPAGASQL
                     TQAEAPKVSIRGSLLQPIIRRADRPTLGDGSLPTGSTGPQVLPH"
     gene            complement(6234..7958)
                     /locus_tag="VN518_05535"
     CDS             complement(6234..7958)
                     /locus_tag="VN518_05535"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HXE04069.1"
                     /translation="MRRHIARLRDSGNFAWSVAASLISLIAIAPVLAIFVLALQSSGD
                     TWPHLIANVLPGALRRTLELMAGVGALTLLIGTGTAWLVTMYRFPGRSLFQWLLLLPL
                     AIPTYIIAFCYLELFDYSGALQTLLRELFGWHSAKDYWFPDIRSVGGAIFVMSMVLYP
                     YVYITARASFLAQSVCVLEVSRTLGLSASATFFEVALPLARPALAAGVALALMETLND
                     IGAVEFFGVRTLTVAVYDTWLDRNSLAGAAQIACIMLAFVFAVLVIERAMRANRRYHH
                     TTGRYRHLPEDRLPGAAGVLAAAACALPVLFGFLSPASVLIHDALAHIAAGLAPEFWD
                     AVLHSLTLSASAACLAVTFAVILGYARRQTRSKLIHTLSIIPAISYAVPGTVLAIGLL
                     IPLAGLDNSVDAMMRSLFGVTTGLLLSGSAFAMVLAYAIRFLAASLGAVEAGLSKISR
                     NTDAAARTLGATISETLWRVHLPLLRPALGAAALLVFVDSMKELPATLLLRPFNFDTL
                     ATQVFTLASLYRYEEAGLSALTIVVASLAPVLLLHGIIVAGRPGALSSRRAAASGGGA
                     AEGLSIAQ"
     gene            complement(7979..>8366)
                     /locus_tag="VN518_05540"
     CDS             complement(7979..>8366)
                     /locus_tag="VN518_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE04070.1"
                     /translation="ETGWNAYARVLGAERAKRAVTELACWVRAVRTSASRKIEYYPFG
                     CAGFCRDECMAISLIAASQHHRCPAMRACALALTGSSLVEPVIDAANAFADALQEADQ
                     LLSPEAVAALAAPLVAAGRDCRTLRN"
BASE COUNT         1499 a         2613 c         2861 g         1393 t
ORIGIN      
        1 ggtcttggca agccgcgccg agacgccgac gcggagcgcg ccggtcaaga gcttgagcag
       61 agcccagcgc tccgtggcgt cgaggccgtc gagccaagag ctgagcagcc caggcacctc
      121 ggttggcatg gcgcgcttta gcgtctgcac cacatcatcg agcctcggcg ctggctccga
      181 cgcatcgcgg cccggccaga tcagcgcgat ggtctcggcg gtatcgccga catagtcgcg
      241 tgacaggccg aacagcacgg gatcgacgcg agcctcgatc aatgcgccca agatgcggcg
      301 aagcggcagg cggaagaaca ggccgtcggt cagagcggcc agcgcccaac cgcggtctgg
      361 atcaggtgtc gaggcaaaat aatcggcaag caagcgaagc ttgtcgttgc gcgacggcgt
      421 gtaggccagc cggtccagca atacggaaaa cgccttcacg actcctcgtc ctcgcggccg
      481 atcagcgcaa gcgcgcggcc gtgccgcccg gtcgattgga tgtgatgcag gagcgcgtcc
      541 tcgcggccat gcgtaaccca cacctcgtgc gcgccggttt cgtgaatcgt ccgcgtcagc
      601 tcgcgccaat cggcgtggtc ggagatgacg agcggcagct cgacgccgag ctgccgcgcc
      661 cgggcgcgga cccgcatcca gcctgaggcg aaggcggtca gcgcctcggg catgcgccgc
      721 gaccagcggt cggcgatggc cgaaggcggt gccagcacga cggcgccttt cagctcctcg
      781 cgcttggcgc cggtggcggg cttgagcgcg ccgagctcga ccccgaagct ctcatagatc
      841 tggcagcagg cggcgagcgc gccgtgcaaa tagatcggct ccgcatagcc agcctcgcgc
      901 aggagagcga tgaggcgctg gcacttgccg agcccgtaga cgccgaccac atgcgcccgc
      961 tcggggaact gcttcagcga tttgagcagc ttcgccactt cgcccttggc atccccatgg
     1021 gtgaacaccg gaagggcgaa ggtcgcttcg gtgacgaaca gatcgcaccg gacaagctcg
     1081 aagggaggat tggtcgggtc ggcgctccgc ttgtagtcgc cggagatcac ggcgcggcgg
     1141 ccgcgccact cgatcaccac ctgcgcgctg ccgagcacgt ggccggcagg cacgagcctc
     1201 accgtcacgt cgccgacctg caaggtctcg ccgtagccga gcgcttgggt gccgagcttc
     1261 gccgcgctgc cgagccgcgc cttcatcagg gcgagcgtct cgcgcgtggc aagcacatga
     1321 tcgtggccgg cgcgcgcgtg atctgagtga gcatgcgtga tcaccgcgcg attcaccgca
     1381 ctgtgcggat cgatgtggaa tccgccaggc cggcaataga ggccgtgcgt gccgacgttg
     1441 agccaggact cgaaacgctc aggatgcatg ctggacggac tctactctag attgctgtct
     1501 gatggggtta ctcgccatga ggttgttgta attctcctgg ttcacaatcg agagatgcag
     1561 catagttgaa gcgggcatgg cgcgcgggtg ttggggggtc gcgtcgatca cgcatatagt
     1621 gtggcgtaca cgcgaggcgg cgcgtatcac tgtcctcggt gccggactgc ttgtggcatc
     1681 gcttatctcg gccgcggcac aagaccctca cagcgaaatc ttcaccgggc tcgaggcgag
     1741 caacaacgcc ataagcggct atttgggcgc gggttacgcc ttcggcaagg gactctacga
     1801 gcgcggctgg cgggtccgcg cggtcggcgc ccttgggcac tacgactatc ggggaactct
     1861 gttcggcgcc ggcgccgatc tcggcacgac cttcgacggc gacgcgagct acggcgcagc
     1921 acttatcggc taccagttcc gcacccaagc cttgtttcta aaattgttcg caggcgtcga
     1981 agccgaagat cagagcatca cgccgcgcga tccggagaat tcggtgcaag gcagtgcggt
     2041 cggcctcaag ctaggaaagc tggctcgacc tttcgccgct gtggttcctc tccgtcgatg
     2101 gctcgtacgg aaccgcgttt cagcaatatt ggagcttggc gcgggcgggc tatcgccttt
     2161 cgccacgctt ctcgcttggg cttgaaggcg gcgcgctcgg caacgaggaa tacgacgcgg
     2221 gccgtggcgg cggcttcgtg aaggcaaatc ttcgcagcct ggagcttacg ctctccggcg
     2281 gcttcaccgg aaattatctc gaagacgaac cgagcggcta tgtgtcgctc ggcgtctatc
     2341 gcgccttctg agaagaactg ggtgcactgc cggccccgcc ccggcagtgc tgtcctcatc
     2401 ggcgtggcgt ttcgccgagg gctgaccgga tctacggtca agcgggcgac gcgcccacct
     2461 tggttgtgca ttctgtgcca gagagcctgc cggaatgccc aagcgagaat ctctccgaac
     2521 aagaaggatg acgcaacgac gacggcaaag caaacgggag ttcacactct ccagcatttt
     2581 cttgtcgatg ttctgaatgg cgttccacgt cggaatttgg tgcgcgcgct gccggccttg
     2641 cgcgccttcg ctgcgagcga tgcgccaaac gcgatcgggc cgcaggcacg cgatcaggaa
     2701 ttttgtcatc acgtcagatg cataggccgc atgcgcgtgc atgcgtgcga agcggcatgt
     2761 gttcgagggg actgccgccc gccgccgcga acagcgagac aggccgactg ggccgcagcg
     2821 ctttgtggta gttaggtgac agcacaagcc cctatgctag gggacaattt taccgtcgct
     2881 ggaccggagc cggcttcggc gccacgctag gcacggagcc ggtttgcgcc tccggcgtcg
     2941 agggcttcgc ctccgctggc ttgggctttt cgtcggcggt cgctgccttc gcatcgcctt
     3001 gcttggactt ttcctcggcg gtcgaaggct ttgtctctgt cgctttggat tttgcgtccg
     3061 cggcggaagg ctttccctcc gggggtgcgg cctttgcatc ggtgaccacc ggctgatcgc
     3121 gccaggtttg gaaccgggtg gcaagctccc cgccatgctc gttccaccaa gcctcgtcaa
     3181 agcggagggc tttccggaaa ttgtcgggcg cagtcggcag atagcccttc atgtcgatgt
     3241 cgagcgaagg atgcttgccg acgagcgcga ttgccgattt gcgcatcggt ccataggcga
     3301 tcagcgacgc ttgcgccgcc atctgggccg gctcggtggc aaaggcgata aagcgtttgg
     3361 cctcgtcctt gttggtggca gctttcggga tcgcccacag atcgaggtcg tagacctggc
     3421 cgtcccacat gacgtcgatg cgctggcggg cgccgaccgc ggcgcggaag atgcggccgc
     3481 tatagccggc cgccatcgac gccttgcgcc gcatcagcat ggagatggag tcctccgctt
     3541 tgtcccacca caggatctgc gccttgatct tgtcgagagc cttgaaggcg cggtcgacgc
     3601 cctccggcgt cgcgagctgg gtatagatgt cggcgggatc gacgccgtcg gcgagcagcg
     3661 caagctcgag cgtgtagcgc gggccattag gcagcgcccg ctttcccggg aagcgctgga
     3721 tgtcgaggag gtcggcgatc ttcccgggct gtgccttggt gaaggcctgg tggtcgaagg
     3781 cgattgccgt cgaccaggcg acgctggcga tgccgcaaga cgtgatgccg ccggagagaa
     3841 agtcgtcgct tacgctctga ccgccaggac ctgcagtcag tgtcgaactg tcgatctgtt
     3901 cgagcagccc gccgtggcag agcgcatcga gggcgccctg cgacaggtcg acaatgtcga
     3961 agggcgagga gctggcgccg atcttatctt tgatcgcggc aagcgtgccg tcataggtct
     4021 cggtcttgat cttgacgccg gtcttctgcg tgaacggctg gaaataggcg atctcctgcg
     4081 actggccata ggccccgccc caggtggcga tggagagagt ggcgccgcca ggcttgctct
     4141 ctgtggctgg cgcgtcttgc gcgctggcgc ccggcgaggc gagcgcgagc caggctgcgg
     4201 caagcgccac cgcatagaga cggcaattgg ctggcatgac gctcttcatt ggtgttcggt
     4261 caaaatcact cgccaaggtc aatgcggtaa gacctgcggt cccgtcgagc ccgtcggtag
     4321 gctgccgtcg cccagcgtcg gacgatctgc tctgcggatg atgggctgca acagcgatcc
     4381 gcggatcgag acctttggcg cctccgcttg ggtcaattgc gaagcccctg ccggaccaat
     4441 ggggttcgcc accgtgagac tccaatcgag cgtccgcttc gtcacatcga cgtcgcctgc
     4501 tccggcaatc gtgccttgcg gcgtgcgcat gctcaaggac tggcaccaga tatggcctgt
     4561 gccgagacgg caatcggcat cgagttgatc gaacggggtg gcattgtcgt ggctccaacc
     4621 gtcgtcgttg agcggtgtcg gccccgccaa cagtcgggca aagtcgaccg gcaccacgcc
     4681 gtctttcgct ttaagcttga gggttccggc gagggagccg gtgaattgcg tggaatcgct
     4741 gccttcggcg gcgagctcgg tcttgatatt ggcggcgccg ctgatcgggg ccgcgaggtc
     4801 aagcggctgg agacaggcgt cgatggcaac gtcatccaaa ttggcgacga ggttcacctg
     4861 tttgctcggc cgagcgagat cggcgtcaag ccggccgtcg gctgagccgc cgcacagttc
     4921 gagctcgccg acctcgcttg ccaccacgcc ttgcttggcg ctgacggtga acccgccccg
     4981 tccgagcttg agcgcgccgg cgtcgatggc ggcagccgag atgcgtaagt cagcatcgat
     5041 aaattgcaat agcggccgat tgaaggggaa ggaagccgtc gactccgtgg agggtggtgc
     5101 cccgccattg ttcgaactgg ctttgccgag atagggatcg agcgtcagcc ggtcaaaggc
     5161 gagcgtgcct tcaacgcgcg ggcgcaaccc tctcgcgctg agcgcaagca ggcctacggc
     5221 cttgttgccg tcgagcgtga aggttccgtc gtcgaaggtg agcgccggtc cggcaagatg
     5281 gaaaccgcct gacgccgaga accccttcaa gctgtcgccg ggcggcagcc tgacgccgag
     5341 ccagttgaga aacttgcgcc cgtcgtcgat ctcggcctgc atgtcgccgt cgagcttgaa
     5401 ctcgcccgca taggacgccg tgccgttcaa tctcacgcgg agaggcttgg atgagagcat
     5461 gagcgcgagt ggaaaactct ccaccccgcc gctcgctgtg ggcgatccgc tttccagcga
     5521 gtagcgcacc gtcgtgtcct tgtaggcgaa ggagccaaaa cctgagacgg ctccggtgtc
     5581 cttgccggcg tcgatatggg cgtagatgtc cttgatggcg ccgctgcgat ccgcgggcag
     5641 cgcgatcctg ccgttgcgca cgtgcaatac gcgcaccggc gctccgccaa gcaggctggt
     5701 gaacaggtcc tgtgcaggcg ttgccgccga agcaccggcc agcaacgtat cgcgcaaggt
     5761 gacgcgcggc ttgagcaggc ggagcgcatc gatggtgatg cggccgcgca ggagatcgac
     5821 caggtcgagc gagactttgg cttccctgac ggtcagcgtt tgcaccgcag gaagcttggt
     5881 gctgtcctgc acctcgagct ggccgcgcac cgagacgtca ggcaggaagc gcacgcgcac
     5941 gggcccaatg aatttgacct cgcctcccgt ccacgccgac agctccgagg cgacccgatc
     6001 cccaaagcgg gaggtgtcgc cgatcagcca gggaatgagg gcgatggcga aaacgagaac
     6061 cgcgagcagg ccgacgagaa gccatttcca tggcagctgg ctaggcttca tgccgttaaa
     6121 tccacccgtt ttcttgctgc cccagaagga gaagcgcgac cagttctatg ccgcccgagc
     6181 cccaggaggt tttagcccga ttgggcggag atagcgagcc cgccgcccgc gatttattgg
     6241 gcgatggaaa gcccctcggc cgcccccccg cccgaggccg ctgcccggcg gctgctcaag
     6301 gccccagggc gtcctgcgac aatgattcca tgcaaaagga gaaccggggc gagactggcg
     6361 accacgatgg tgagcgccga cagcccggct tcctcatagc ggtagagcga ggcgagggtg
     6421 aacacctggg tggcaagcgt atcgaagttg aaggggcgga gcagcagcgt ggccggaagc
     6481 tccttcatcg agtcgacgaa gacgaggagg gccgcggcgc cgagcgccgg gcggagcagc
     6541 ggcagatgca cccgccacaa ggtttcgcta atcgtggcgc caagcgtccg cgccgcggca
     6601 tcggtgttgc gcgagatctt gctgaggccg gcctcgaccg cacccagaga cgccgcgagg
     6661 aagcggatgg cgtaggcgag caccatggca aaggcgctgc ctgagagcag gagacccgtc
     6721 gtcacgccga acagcgatcg catcatggcg tcaacgctgt tgtcgagacc ggcaagcggg
     6781 atcaaaagcc cgatggcgag aacggtgccg ggaacggcgt agctgatggc cgggatgatg
     6841 ctcaaagtgt ggatcagctt cgatctcgtc tgccggcgcg catagccgag gatcacggcg
     6901 aaggtgacgg caaggcaagc ggcagaggct gacagcgtca ggctgtgcaa gactgcgtcc
     6961 cagaactccg gcgcgagccc ggctgcgata tgggcaagcg cgtcgtggat cagcaccgag
     7021 gccggcgaca ggaacccgaa cagcaccggg agcgcgcaag ccgcggcagc aagcactcca
     7081 gctgcgccgg gcagcctgtc ctccgggaga tggcgataac gacctgtggt gtggtggtag
     7141 cgccgattgg cccgcatggc gcgctcgatc accagaacgg cgaagacgaa ggcgagcatg
     7201 atgcaggcga tctgcgccgc gcccgccaga ctgttgcggt cgagccaggt gtcgtaaacg
     7261 gcgacggtga gggtgcgcac gccgaagaat tccaccgcgc cgatgtcgtt caaagtttcc
     7321 atcaacgcga gcgctacgcc tgcggcaagc gcgggtctcg ccagcggcaa cgccacctcg
     7381 aagaaggttg ccgatgcgct cagcccgagc gtgcggctca cctcgagcac gcaaaccgac
     7441 tgcgcgagaa agctcgcccg cgcggtgatg tagacgtaag ggtagagcac catgctcatc
     7501 acgaagatgg cgccgcccac gctcctgatg tcgggaaacc agtagtcctt ggcgctgtgc
     7561 cagccgaaca gctcacgcaa cagggtctgc agcgcgccgg aatagtcgaa cagctcgaga
     7621 tagcaaaacg cgatgatgta ggttgggatg gcgagaggca ggagcagcag ccactggaac
     7681 aggcttcgcc ccgggaagcg gtacatggtg acgagccacg ccgtgccggt gccgatcaag
     7741 agggtgagcg cgccgacgcc ggccataagc tcgagcgtgc ggcgtaacgc gcctggcagc
     7801 acgttggcga tgagatgcgg ccaggtatcg ccgctcgact ggagcgccag cacgaagatg
     7861 gcgagcaccg gggcaattgc gatcagcgag atgagagagg ccgccaccga ccaggcgaaa
     7921 ttgccgctgt cgcgcaatcg cgcgatatgg cgacgcataa agtttggacg cgatccgctc
     7981 agttgcgcag cgtgcggcaa tcgcggcctg cggcgactag cggtgcggcg agcgcggcga
     8041 cggcctcagg cgagagcagc tggtcggcct cctgcagggc atcggcaaag gcgttggcgg
     8101 cgtcgatgac cggctcgacc aggctcgatc cggtcagcgc cagcgcgcaa gcgcgcatcg
     8161 ccgggcagcg atggtgctgc gaggcggcga tcagcgaaat cgccatgcac tcgtcgcggc
     8221 agaagccggc gcagccgaac gggtaatatt cgatcttgcg cgaggccgag gtccgcacag
     8281 ctctcaccca gcaggcaagc tcggtcaccg cccgcttcgc ccgttccgcg ccgagcacgc
     8341 gggcataggc attccacccg gtctcc
//
LOCUS       DATNAE010000244         3729 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3547676, whole genome shotgun sequence.
ACCESSION   DATNAE010000244 DATNAE010000000
VERSION     DATNAE010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3729
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3547676"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..2810)
                     /gene="ppdK"
                     /locus_tag="VN518_05545"
     CDS             complement(108..2810)
                     /gene="ppdK"
                     /locus_tag="VN518_05545"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HXE04071.1"
                     /translation="MSRVEPVPEPAPRPATKWVYSFGAGRADGSAADRDRLGGKGAYL
                     AEMSRLGLPVPPGFTISAELCSVYYELGRKLPEELKPMVSAALDEVGKIVGAGFGDVH
                     NPLLVSVRSGSRASMPGMMDTVLNLGLNDQTVEGLAVRSGDRRFAYDTYRRFIQMYAD
                     VVLGLDHELFEEILENYKGLKGLEYDTELGAENWSDIVARFKTLVENELGTPFPQDIH
                     AQLWGAIAAVFGSWQNARAIAYRRLHDIPDDWGTAVSIQAMVFGNMGEKSATGVVFTR
                     NPSTGANELYGEFLVNAQGEDVVAGLRTPEPLTEAARRAANVKRPSLEALMPAAFAEL
                     KAACAKLEAHFHDIQDIEFTVQDGKLFMLQTRSGKRSTQAALKIAVDMAEEALITREQ
                     AVQSINADQLDQLLHPTLDPTAEKTVLAKGLPASPGAASGELVFDADEAVHLKAQGHT
                     VILARVETSPEDVQGMHAAVGILTTRGGMTSHAAVVARGMGRPCVVGASAVSIDLERE
                     TLQAGGTLLQKGDIITIDGSTGQIIKGRVAMREPDLSEDFNRLMRWADGFRRLKVRAN
                     ADTPADARQALAFGAEGIGLCRTEHMFFEESRILAMREMILAEDQEGRRKALAKLLPA
                     QRMDFVALFEIMAGLPVTIRLLDPPLHEFLPHGDEVIGEVAADMGVSVARLRRRIVAL
                     SEFNPMLGQRGARLLVSYPEIVEMQARAIFEAAIEAGKALHDRVIPEIMVPLVATKGE
                     LDLVKDRIAQVARAVEKERGTNVAYSVGTMVELPRACLMAGEIGRSAEFFSFGTNDLT
                     QTTFGLSRDDAGRFLGEYADKGIIQHDPFVTLDEAVGELMQIAVDRGRAVKPELKMGI
                     CGEHGGDPETIGFCEKVALNYVSCSPYRVPVARLAAAQAALRNR"
     gene            complement(2906..3349)
                     /locus_tag="VN518_05550"
     CDS             complement(2906..3349)
                     /locus_tag="VN518_05550"
                     /inference="COORDINATES: protein motif:HMM:NF017868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HXE04072.1"
                     /translation="MPEHLFVYGTLRAESAHPMAHRLRVGARHIGNGRAPGLLYDFGS
                     WPGAFFALEEKYRVIGSVFALGPNPRLLSDLDKYEGVVPTDEEREFPESAGLFHRIAI
                     EVHLDHGGSVEAWAYALKDTPRARLIGTGDFIADRKLRAPKVRRS"
     gene            complement(3358..>3729)
                     /locus_tag="VN518_05555"
     CDS             complement(3358..>3729)
                     /locus_tag="VN518_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXE04073.1"
                     /translation="RFYERLGFVKSKSASQDEVSFFNAGGVVLALWGRDAQIEDAAAH
                     GLWNGNGGIVVARNVASEAEVDAAMATAETAGARMLKPAAKTFWGGYNGYFADPDGNL
                     WEVAFNPHWGLEEDGRIKLPD"
BASE COUNT          657 a         1229 c         1188 g          655 t
ORIGIN      
        1 cttcaatgca ctgtagataa acggaaaaaa tccctgggcc gtttaccttg tcatgggcag
       61 aaatggctca acatggccat tttttgatgg caagcggtaa ttatcggtta gcggttccgg
      121 agcgccgctt gggccgcggc aaggcgggcg accggcactc ggtagggtga gcaggacaca
      181 taattcagcg cgactttctc acagaaccca atggtttccg ggtcgccgcc atgctcgccg
      241 caaatcccca tcttgagttc gggcttcacc gcgcgacctc gatccaccgc gatctgcatc
      301 aattcgccca cagcttcgtc cagggtgacg aagggatcgt gctggatgat gcccttgtcg
      361 gcgtactcgc ccaagaatcg gccggcatcg tcgcgactca gcccgaacgt ggtctgggtc
      421 aggtcgttgg tgccgaagga gaagaactcg gcgctcctgc cgatctcgcc ggccatgaga
      481 caggcgcgag gaagctcgac catggtgccg accgaatagg cgacgttggt cccccgctcc
      541 ttctccaccg cacgcgccac ttgggcgatg cggtccttga ccaggtcgag ctcgcccttg
      601 gtggcgacca gcggcaccat gatctcgggg atgacacggt cgtgcagcgc cttgccggcc
      661 tcgatcgccg cctcgaagat ggcgcgggcc tgcatctcga cgatctcggg gtagcttacg
      721 agcagccgcg cgccgcgctg accgagcatg ggattgaact cgctgagcgc gacgatgcgg
      781 cggcgcaatc tggcgacgct cacccccatg tcggcggcga cctcgccgat gacctcatcg
      841 ccatgcggca ggaactcgtg cagcggcgga tcgagcaggc ggatggtcac ggggagtccc
      901 gccatgatct cgaacagcgc gacgaagtcc atccgctgcg ccggcaacag cttggcgagc
      961 gccttgcggc ggccctcctg atcctcggcg agaatcatct cgcgcatggc gaggatgcgg
     1021 ctctcctcga agaacatatg ctcggtgcgg caaaggccga tgccctcggc gccaaaggcg
     1081 agcgcctggc gcgcgtcggc cggcgtgtcg gcattggcgc gcactttcag ccggcggaaa
     1141 ccgtccgccc accgcatcag gcgattgaag tcctccgaga gatccggctc gcgcatcgcc
     1201 acgcggccct tgatgatctg cccggtcgag ccgtcgatgg tgatgatgtc gcccttctgc
     1261 agcagcgtgc cgccggcttg cagcgtttcg cgctcgagat cgatcgagac ggctgaagcc
     1321 ccgaccacgc aaggccggcc catgccgcgc gccacgaccg ccgcatggct cgtcatgccg
     1381 ccccgcgtgg tgaggatccc gacagcggca tgcatgcctt gcacgtcctc gggcgaggtt
     1441 tcgacacggg cgaggatcac agtgtggcct tgcgccttga gatgcacggc ctcgtcggcg
     1501 tcgaacacca gctcgccgga agcggcgccg ggggaagcgg gcaagccctt ggcgagcacg
     1561 gtcttctcgg cggtgggatc gagcgtcgga tgcagcagct gatcgagctg gtcggcattg
     1621 atgctctgca ccgcctgctc gcgggtgatc agtgcctcct cggccatgtc gacggcgatc
     1681 ttgagagccg cctgcgtcga gcgcttgccc gagcgggtct gcagcatgaa cagcttgccg
     1741 tcctgcacgg tgaactcgat gtcctgaatg tcgtggaaat gcgcttcgag cttggcgcaa
     1801 gccgccttca gctcggcgaa ggcggcaggc atgagcgctt cgagcgaggg acgcttcacg
     1861 ttcgcggcgc ggcgcgccgc ttcggtcaac ggctcgggtg tacgcaaccc cgccaccaca
     1921 tcctcgcctt gcgcattgac caggaactcg ccgtaaagct cgttggcgcc ggtcgaaggg
     1981 ttgcgcgtga acacgacgcc ggtcgcgctc ttctcgccca tattgccgaa taccatggcc
     2041 tggatgctga ccgcggtacc ccaatcgtcg ggaatgtcgt gcaggcggcg ataggcgatg
     2101 gcgcgcgcat tctgccaaga gccgaacacg gcggcgatgg cgccccagag ctgggcgtga
     2161 atatcctgcg ggaagggcgt gccgagctcg ttctcgacca gggtcttgaa gcgggcgacg
     2221 atgtcgctcc aattctcggc gccgagctca gtgtcgtatt ccaagccttt gagacctttg
     2281 taattctcca ggatctcctc gaacagctca tggtcgaggc cgagcaccac gtcggcatac
     2341 atctggatga agcggcgata ggtatcgtag gcgaagcggc ggtcgcccga gcggaccgcc
     2401 aagccctcga cggtctgatc gttgagcccg agattgagca cggtatccat catgccgggc
     2461 atcgaggcgc ggctgcccga cctgaccgac acgagcagcg gattgtgcac atcgccgaag
     2521 ccagcgccga cgatcttgcc cacctcgtcg agcgccgcgc tgaccatcgg cttcagctcc
     2581 tcgggcagct tgcggccgag ctcgtaatag accgaacaca gctcagccga gatggtgaag
     2641 cccggcggta cgggaaggcc aagccgcgac atctcggcga gataggcgcc cttgccgccc
     2701 agccgatcgc gatcggcggc cgagccgtcg gcgcggccgg cgccgaagct gtacacccat
     2761 ttcgtcgcgg gtcttggcgc tggctcgggc acgggctcca ctctcgacat tgctcgttcc
     2821 atattgccgg acggtccggt gacgccgggt ccgacctttt aacggtccct cggcgcagat
     2881 acaaaccgct cgcgatggac aagggttaag agcggcggac cttcggcgcg cgcaatttgc
     2941 ggtcggcgat gaaatcgccg gtgccgatca gcctggcccg aggcgtgtcc ttcaaggcat
     3001 aggcccaagc ttcgacgctg ccgccgtgat cgagatgcac ctcgatggcg atgcgatgaa
     3061 acagtcccgc ggactccgga aactcgcgtt cctcgtcggt cggcaccacg ccttcatact
     3121 tgtcgaggtc ggacaggagg cgcgggttcg gaccgagggc aaacacgctg ccgatcaccc
     3181 ggtatttctc ctcgagtgcg aaaaacgcac cgggccagct gccgaaatcg tagagcaggc
     3241 caggggctct gccgttgccg atatgcctcg cccccacgcg caggcggtgc gccatcgggt
     3301 gcgcgctctc ggcccgcagc gtgccataga cgaagagatg ttccggcata gagatcatta
     3361 atccggcaat ttgatccgtc cgtcctcctc caacccccaa tgcggattga aggcgacctc
     3421 ccacagattc ccatcagggt cggcaaagta tccgttatag ccgccccaaa aggtcttggc
     3481 cgccggcttc aacatgcgcg cgccagccgt ttcggccgtt gccatagcag cgtcgacctc
     3541 ggcctcgctt gcgacatttc gcgcgacgac gataccgcca ttgccgttcc acagcccatg
     3601 agcagcggcg tcctcgatct gcgcgtcgcg cccccacagc gcgagcacga cgcctcccgc
     3661 attgaaaaag cttacctcgt cctggctggc cgatttggat ttcacaaagc caaggcgctc
     3721 gtagaagcg
//
LOCUS       DATNAE010000245         2035 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3548219, whole genome shotgun sequence.
ACCESSION   DATNAE010000245 DATNAE010000000
VERSION     DATNAE010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3548219"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..1454)
                     /locus_tag="VN518_05560"
     CDS             complement(138..1454)
                     /locus_tag="VN518_05560"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HXE04074.1"
                     /translation="MQPYPPEQPSRTVALINARLLDPASRLDRQGGLLIRDGLIVDFG
                     SHVAEGVAGDAEIIDCGGHLLAPGLVDMMVFTGEPGHEHRETLATASRAAAAGGVTTI
                     VCMPNTDPVIDDVALVDFILRRARDTALVNVHPMAAMTKGLLGEEMSEIGLLRDAGAI
                     AFANGKASLANAKVMRNVLAYAKDFGALIVHHLEDPWLAKDGVMNEGEVATRLGLRGI
                     PSAAETIMLERDIRLVELAGGRYHAGQISCRASLDIIRTAKLMGLPVTCGVSINHLTL
                     NENDIGPYRTFFKMKPPLRSEDDRRAMVEGLASGDIDVIVSAHDPRGAEGKRRPFSEA
                     ADGAVGLETLLGAALRLYHNGEIELGRLLQALTVNPARLLKLGSGRLAKGAPADLVLI
                     DLDRPWVVEPEALRSKSKNTPFDAARLQGRALLTFVAGRLVYNYAE"
     gene            complement(1460..>2035)
                     /locus_tag="VN518_05565"
     CDS             complement(1460..>2035)
                     /locus_tag="VN518_05565"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041536035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HXE04075.1"
                     /translation="SQKVDCSVINAGDGSHEHPTQALLDALTIRRHKGQIEGLIVAIC
                     GDILHSRVARSNILLLNTLGARVRVVAPSTLLAAGIERLGVEVYTSMWDGLADADIVM
                     MLRLQRERMTSSLVPSTREYFHFFGLDYDKLARAKPDALIMHPGPMNRGVEIDSNVAD
                     DARSVIREQVEMGVAVRMAVLQALARHLPNE"
BASE COUNT          375 a          634 c          691 g          335 t
ORIGIN      
        1 aggatgagtc cgaaggggat ggaaccgagc agatagccga acccgaagct caagaaacaa
       61 agggcgagga gcaaggggag ggcttgttcc caagcctcgg aaggcggcat cgatcaccct
      121 ctttgaacgc cgacagatta ctcagcgtaa ttgtagacga ggcgaccggc aacgaaggtc
      181 agcagtgccc ggccctgaag cctcgccgca tcgaacggcg tgttcttcga tttcgaccgc
      241 aacgcttccg gctcgaccac ccagggccgg tcgagatcga tcagcacgag atcggcgggc
      301 gcgcccttgg cgagccggcc gctgccaagc ttgagcaggc gcgccggatt taccgtgagc
      361 gcttgcaaga gccgcccaag ctcgatctcg ccattgtggt agagacggag cgcggcgccg
      421 agcaaggtct cgaggcccac ggccccgtcg gcggcctcgg aaaatgggcg gcgcttgccc
      481 tcggcgccgc gtggatcgtg ggccgagacg atcacatcga tatcgccgct cgccaagcct
      541 tcgaccatgg cgcgacgatc gtcctccgat cggagcggcg gcttcatctt gaagaaggtg
      601 cggtagggac cgatgtcgtt ctcgttcagc gtcaggtggt tgatcgacac cccgcaagtg
      661 acgggcaagc ccatcagctt cgccgtgcgg atgatgtcga gcgaggcgcg gcaggagatc
      721 tggcccgcgt gatagcggcc gcccgcaagc tcgaccaggc gaatgtcgcg ctcgagcatg
      781 atggtctcgg cagccgaagg gatgccgcga agaccgagcc tggtcgcgac ctcgccctcg
      841 ttcatcacgc cgtccttggc gagccagggg tcttccaaat gatgcacgat cagcgcgccg
      901 aaatccttcg cgtaggcgag cacgttgcgc atgaccttgg cgttggcgag gctggctttg
      961 ccgttggcga aggcgatggc gcccgcatcc cgcagcagtc cgatctcgct catttcctcg
     1021 ccgagcagac ccttggtcat ggccgccatc ggatgaacgt tcaccaaggc ggtgtcgcgg
     1081 gcgcgccgca ggataaagtc gaccagagcc acgtcgtcga tcacgggatc ggtgttcggc
     1141 atgcagacga tagtcgtgac cccgccggcg gccgcggcac ggctcgccgt ggccagcgtc
     1201 tcgcgatgct catgcccggg ctcgccggta aacaccatca tgtcgaccag accgggggcg
     1261 agcaggtggc cgccgcaatc gataatctcg gcatcgcctg cgacgccttc agctacatgc
     1321 gatccgaaat cgacgatgag gccgtcccgg atcagcaagc ctccttgacg atccagccgc
     1381 gaggcagggt cgagcagacg cgcattgatc aaggcgacgg tgcgcgaggg ttgctctggc
     1441 ggatagggtt gcatcgtcgt cattcgttcg gcaggtggcg cgcgagcgcc tgcagcacgg
     1501 ccatgcggac cgccacgccc atctccactt gctcgcggat gacgcttcgg gcgtcgtcgg
     1561 cgacattgga gtcgatctcg acgccgcggt tcatcgggcc aggatgcatg atgagagcgt
     1621 ccggcttggc gcgggcgagc ttgtcgtaat cgaggccgaa gaaatggaaa tattcgcggg
     1681 tgctcggcac caggctcgag gtcatgcgct cccgctgaag cctcagcatc atgacgatat
     1741 cggcatcggc caggccgtcc cacatcgagg tatagacctc gacgccgagc cgttcgatgc
     1801 cggcggcgag cagcgtcgac ggcgcgacga cgcggacgcg ggcgccgagc gtgttgagca
     1861 gcaagatatt ggagcgcgcc acgcgggagt ggagaatgtc gccgcagatg gcgacaatca
     1921 ggccctcgat ctgacccttg tggcggcgga tggtgagtgc gtcgagcaaa gcttgcgtcg
     1981 ggtgctcgtg gctcccgtcg ccggcgttga tcacggagca atcgaccttc tgcga
//
LOCUS       DATNAE010000246         4065 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_1440780, whole genome shotgun sequence.
ACCESSION   DATNAE010000246 DATNAE010000000
VERSION     DATNAE010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4065
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1440780"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VN518_05570"
     CDS             complement(<1..318)
                     /locus_tag="VN518_05570"
                     /inference="COORDINATES: protein motif:HMM:NF014599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HXE04076.1"
                     /translation="MAKKTESPEQSGKRNGDGMPGGDGKSGGQAPSPEAAAPESAAPG
                     AGAQLNVLGQYIKDLSFESPHAPQTLQNPPPNPQLQVTVNVNVASQSPETYEVVLNID
                     VHAK"
     gene            609..1337
                     /locus_tag="VN518_05575"
     CDS             609..1337
                     /locus_tag="VN518_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008550406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44/TimA family putative adaptor protein"
                     /protein_id="HXE04077.1"
                     /translation="MSEAFDIYTLLFLVLAVVIFIRLRNVLGRRTGNERQPFDPYTTP
                     DQKRPQAPKTNEPVVALPRGRSAPLPEATGPSVEDIEARLGSHAPKDSPLARSLTQLM
                     RADPSFDPNQFLDGAKTAYEMIVMSFAEGDEASLKQLLSAEVFEGFESAIRERETRGE
                     KVESNLVGINKADIIESDVKKNTAYVTVKFVSELISVTRDAEGEVVEGDPKKVREVTD
                     IWTFARDISSKNPNWKLVATESAN"
     gene            1312..2517
                     /locus_tag="VN518_05580"
     CDS             1312..2517
                     /locus_tag="VN518_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HXE04078.1"
                     /translation="MSRPNRRTEPYPGAASFYGAIAAALLLAAIAATIAIYLLLRETR
                     VEETGISFEPVTFADIDGWQDDDQAAAFQALLRSCGRMKRDSASAEACAAARDLAAHG
                     QVSRDAARTFFETQYTPNRIVGAPNKGLVTGYYEPEIDGAREKSDKFPAAAYGRPDDL
                     VSVKPDETRASDSAAGVLTAMRKAPDGLVPFYTREEIDKGALAGKGLELVYLDSVELF
                     FMQVQGSGRVRLPDGTHVRLGYATKNGHPYTSIGKKLLELGEDKPKSMTMQGIKDWLR
                     ADKERGNKMMWENKSYVFFRLLDGKEGQDGPIGAQGVSLTPGRSLAVDPSFHALGLPV
                     FVEAPAMKGDGAPFRRLMIAQDVGSAIKGVERGDIYWGSGDDAGAIAGKTLAPAEFIV
                     LLPNAAPGV"
     gene            2521..3087
                     /locus_tag="VN518_05585"
     CDS             2521..3087
                     /locus_tag="VN518_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020179397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HXE04079.1"
                     /translation="MGKRGQGDNDDAALWEKVVETAAPLKRGRNTAAPKPAKIAAPVA
                     KAKPPQGPRPTRPAPKPAPKPSRVPRAAPLDRQTSRQLDKGKLEVEARLDLHGMRQRD
                     AHAQLRRFLKAAQAQGLKHVLVITGKGADQAASRSFYEEDERGVLRNAVPHWLAEPEL
                     GALVVSYSPAPRRLGGGGALYVRLRRAR"
     gene            complement(3339..3770)
                     /locus_tag="VN518_05590"
     CDS             complement(3339..3770)
                     /locus_tag="VN518_05590"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXE04080.1"
                     /translation="MVLAHGAWADGSSWARVITALEAESAKVVAPPLPLTSLADDVAA
                     LNRSLDRTEGPIVVAGHAYAGAVIALARPERVKALVYVTALAPDEGEKVADVFYRLEP
                     HPQAPKLAPDSNGLIWLPEEAFASAFAQNASVDAPCSGPYR"
BASE COUNT          733 a         1286 c         1325 g          721 t
ORIGIN      
        1 cttggcgtgg acgtcgatat tcaacaccac ctcataggtc tctggcgact ggcttgccac
       61 gttgacattg acggtgactt gcaactgcgg atttggcgga ggattttgca gcgtttgcgg
      121 cgcgtgcggg ctctcgaagg agaggtcctt gatgtattgg ccgagcacgt tgagctgggc
      181 gcctgcgcct ggcgcggcgg attcaggggc cgcggcctcc gggcttggcg cttgaccacc
      241 cgacttgccg tcgcctccag gcatgccatc accgttgcgc ttgcccgatt gttcagggct
      301 ttcagtcttc ttggccatga ggcttaggtc cggcttggag gaggggcctg ctggctagca
      361 tggcatgccc aggccctgca atcttgccgc atggtacccg cttcttcccg aatttccttc
      421 tcgccttttt tgggggttgg ctaaggtgcc gccaatgcct taagttgagc tttgcgcgac
      481 atgggtgagg tggttgagtc ccacaacgcc tgaggctccc gctagcaaat ctccaggcga
      541 gaaccaatat gagcctgggg agttccgcat catcgcgggt ctatccatca acgcgggtgg
      601 cggcaaggat gagtgaagcc ttcgatatct atactttgct gtttctggtg ctggccgtgg
      661 tgatctttat ccggctgcgt aatgtgctcg gccgccgcac cggcaacgag cgccagccgt
      721 tcgatcctta tacgacgccg gatcagaagc ggccgcaagc gccaaagacg aatgagccgg
      781 tggtcgccct gccgcgcggc cgctctgccc ccttgcccga ggccacgggg ccgtcggtcg
      841 aggatatcga ggcgaggctc ggcagccatg cgccgaagga cagcccgctc gcccgctcgc
      901 tcacccagct gatgcgcgcc gatccgagct tcgatccgaa ccaattcctc gacggcgcca
      961 agaccgccta cgagatgatc gtcatgtcgt ttgccgaagg cgacgaagcc tcgctgaagc
     1021 agctcttgag cgccgaggtg ttcgagggct tcgaaagcgc cattcgcgag cgcgagacgc
     1081 gcggcgagaa ggtcgagtcg aacctcgtcg gcatcaacaa ggccgacatc atcgagtccg
     1141 acgtgaagaa gaacactgcc tacgtcaccg tcaaattcgt gagcgagctc atctcggtga
     1201 cacgcgacgc cgaaggcgag gtggtggaag gcgacccgaa gaaggtgcgc gaggtcaccg
     1261 acatctggac cttcgcgcgc gacatcagct caaagaaccc gaactggaag cttgtcgcga
     1321 ccgaatcggc gaactgaacc ctacccaggc gctgcctcgt tctacggcgc catcgccgcc
     1381 gcattgctgc ttgccgcaat cgctgccacc atcgcgattt atctgctgct gcgggaaaca
     1441 cgcgtggaag agactggcat cagcttcgag ccggtgacct ttgccgacat cgatggctgg
     1501 caggacgacg accaggcggc tgcctttcag gccttgctca ggtcgtgcgg cagaatgaag
     1561 cgcgacagtg cttcggcaga ggcgtgcgcc gcggcgcggg atctcgccgc acatgggcaa
     1621 gtgtcgcgcg atgccgcgcg gacctttttc gagacgcaat acacgccgaa cagaatcgtc
     1681 ggcgcgccga acaagggtct tgtcaccggc tattacgagc ctgagatcga cggcgcgcga
     1741 gagaagagcg acaagtttcc ggcagcggcc tatgggcgcc cggacgatct cgtttcagtg
     1801 aagccggacg agacgcgcgc gagcgactcg gccgccggtg tcctgaccgc gatgcgcaaa
     1861 gcaccggacg ggctcgttcc cttctacacg cgcgaggaga tcgacaaggg cgcgctcgcc
     1921 ggcaaggggc ttgagctcgt ctatctcgat tcggtcgagc tgttcttcat gcaggtgcag
     1981 ggctccggcc gcgtgcggct tcccgacggc acgcatgtgc ggctcggtta tgccaccaag
     2041 aacggccatc cctatacgtc gatcggcaag aagctgctcg agctcggcga ggacaagccg
     2101 aagtcgatga cgatgcaggg catcaaggat tggctgcgcg ccgacaaaga gcgcggcaac
     2161 aagatgatgt gggagaacaa gtcctatgtg ttcttccgcc tgctcgacgg caaggagggg
     2221 caggacggcc cgatcggcgc gcagggcgtg tcgctcacgc ccggacggag cctagccgtc
     2281 gatccgtcgt ttcacgcgct tggccttccg gtgttcgtcg aggcgccggc gatgaaaggc
     2341 gacggcgcgc cgttccgccg cttgatgatc gcgcaggacg tgggctccgc catcaagggc
     2401 gtggagcgcg gcgacatcta ttggggctcc ggcgacgacg ccggcgccat cgccggcaag
     2461 accttagcgc cggcagagtt catcgtgctt ctgccgaacg cggcgcctgg ggtgtgattc
     2521 atgggcaagc gcgggcaagg cgataatgac gacgccgcgt tgtgggagaa ggtcgtcgag
     2581 accgccgcgc cgctgaagcg gggccgcaat acggcggcgc caaaacctgc caagattgcc
     2641 gcgcccgtgg cgaaggccaa gccaccgcag ggcccacgcc cgacacggcc cgcacctaag
     2701 cccgcgccga agccaagccg tgttccacgc gccgcgccgc tcgatcggca gacgtcgcgc
     2761 cagctggaca aaggcaagct cgaggtcgaa gcccgattag acttgcacgg catgcgccag
     2821 cgcgacgcgc atgcgcagct tcgccgcttc cttaaggccg cgcaggcgca ggggctcaag
     2881 catgtgctgg tgatcaccgg caagggcgcc gatcaggctg cgagcaggag cttctacgag
     2941 gaggatgagc gcggcgtgct acgcaacgcc gtgccgcact ggctggccga gcctgagctc
     3001 ggtgcgctgg tggtgagcta ttcgcctgcc ccccgccgcc tcggcggcgg cggcgcgctt
     3061 tacgtgaggc tgcggcgggc taggtgaacc gtccctcgtc agggctcgct ctcagcttcc
     3121 ggttatggag cggatagcgt cccggatcac gtctacgaca gccatgggtg cggtaacgct
     3181 gggtatgtgg tcggcagcat gggcctttat cttcgccttc atgcgctctg ccatgtagag
     3241 ctgggtccca gggacgatca tccgatcctc ttcggcaagc agaaaccagc tcgggatgtc
     3301 ccgccacagc gggcgcccga caggaacagt aatgcagctc agcgatatgg gccgctgcac
     3361 ggcgcgtcca ctgatgcatt ctgcgcgaaa gcgctcgcga aggcttcttc gggaagccag
     3421 atcagaccat tgctgtctgg tgcaagcttt ggagcttggg gatgaggttc gaggcgataa
     3481 aacacgtctg cgaccttctc gccctcgtcg ggagcaagag cggttacgta gaccagcgcc
     3541 ttcacacgtt cggggcgtgc gagcgcgatg accgcaccgg cataagcatg tcccgcgact
     3601 acgatcggcc cctcggtccg gtccaagctg cggttgagcg cggccacgtc gtcagcaagc
     3661 gaggtcagcg gaagcggcgg cgcgacgacc ttggccgact ccgcctcgag tgccgttatg
     3721 acacgtgccc agcttgatcc atccgcccat gctccgtggg caagcaccac gctgacatct
     3781 ctcgctgcca tgctgccgct ccaaccagtg cttcgagtcg agcagaacct caaagagccg
     3841 ccttatttgg ccggccggcc cttctcctcg acgacgaatc cgaggtgtcc gagcaccctt
     3901 acggcaccag ccttcccgcc ctcgaagtca ctcgagcccc gagacgcttg cccgttgcga
     3961 actcataggc tgtgcccaag attgctttcg gcgggtaacg gcgtccttcc caagcgacct
     4021 cataggtcgt ggtaggggcg aagccatgct cagagaagaa ccgct
//
LOCUS       DATNAE010000247         2053 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_848592, whole genome shotgun sequence.
ACCESSION   DATNAE010000247 DATNAE010000000
VERSION     DATNAE010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848592"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1850)
                     /locus_tag="VN518_05595"
     CDS             complement(<1..1850)
                     /locus_tag="VN518_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HXE04081.1"
                     /translation="MAAADDPPFDLPAAAPEAPRRPISARALQGSAAVPSPPYLDDLN
                     PEQRAAVEALDGPVLVLAGAGTGKTRVLTTRIAHILGTRRAFPGQVLSVTFTNKAARE
                     MKERIGNLVGGAVEGMPWLGTFHSIGVKILRRHAELVGLKSGFTILDTDDQIRLLKQV
                     IEAEGLDKDRWPARQLASLIDSWKNRGLTPDKVPAGEAYGFASGKGAQLYAAYQRRLK
                     ELNACDFGDLLLENLRLFLDHPDVLADYQRRFKYILVDEYQDTNVAQYLWLRLLAQGS
                     PNICCVGDDDQSIYGWRGAEVDNILRFEKDFPGARVIRLERNYRSTGHILGAAAGLIS
                     ANQGRLGKTLFTDGEHGTKVTVTAVWDDEEEARTIGDDIEAMQKRVGQPLNEVAILVR
                     ASFQMRAFEDRFITMGLPYRVIGGPRFYERQEIKDAIAYLEIAANPANDLKFERIINV
                     PKRGLGDTSVKRVHELARARSIPMYQAAQMIIETEELPGKARKSLADLVASFERWRSH
                     ISHMKHTELAELILDESGYTAMWQADKSPQAQSRLENLKELVRFMHEFDTLEGFLEHV
                     SLVMDADQAGDGQRVSVMTLHAAKGLEFEVVFLPGWEEGLFPHQRSLDESG"
BASE COUNT          327 a          640 c          704 g          382 t
ORIGIN      
        1 ccgctctcgt cgagcgagcg ctggtgcggg aagagaccct cctcccagcc gggcaggaac
       61 accacctcga actccaggcc cttggcggcg tgcagcgtca tcactgatac gcgctggccg
      121 tcgcccgcct ggtcggcgtc catgacgagg ctgacgtgct cgagaaagcc ttcgagcgtg
      181 tcgaactcgt gcatgaagcg cacgagctcc ttcaagtttt cgagccgcga ttgggcctgc
      241 gggctcttgt ccgcctgcca catcgccgtg tagccggact catccaggat caactcggcc
      301 agctcggtgt gcttcatgtg gctgatgtgg ctgcgccagc gctcgaagct agcgacgaga
      361 tcggcaaggg atttgcgcgc cttgcccggc aactcctccg tctcgataat catctgcgcc
      421 gcctgataca tcgggatcga gcgggcgcgc gctaattcgt gcacgcgctt caccgaggta
      481 tcgccgaggc cgcgcttggg cacgttgata atgcgctcga acttcaaatc gttggccgga
      541 ttggcggcga tctcgagata ggcgatggcg tccttgattt cctggcgctc gtagaagcgc
      601 gggccgccga tgacacggta gggaagcccc atggtgatga agcggtcttc gaaagcgcgc
      661 atctggaacg aggcacgcac taggatcgcc acctcattca gcggctggcc cacgcgcttc
      721 tgcatcgcct cgatgtcatc gccgatggtg cgcgcttcct cctcgtcgtc ccacacggct
      781 gtgacggtca ccttggtgcc gtgctcgccg tcggtgaaca gcgtcttgcc gaggcgcccc
      841 tgattggccg agatgagccc ggcggcggcg ccgaggatgt ggccggtcga gcggtagttg
      901 cgttcgagcc gaatgacgcg cgctcccggg aagtccttct cgaagcgcag gatgttgtcg
      961 acctcggcgc cgcgccagcc gtagatcgac tggtcgtcgt cgccgacgca gcagatgttg
     1021 ggcgagcctt gcgcgagcaa ccgcagccat aggtactggg caacgttggt gtcctggtac
     1081 tcgtcgacca ggatgtattt gaatcggcgc tggtagtcgg cgagcacgtc ggggtggtct
     1141 agaaacagcc gcaggttttc gagcagcaga tcgccgaagt cgcaggcgtt gagctctttg
     1201 aggcgccgct ggtacgcggc gtagagctgc gcgcccttcc ccgacgcaaa gccgtaggct
     1261 tcgcccgctg gcaccttgtc gggcgtcagt ccgcggttct tccagctgtc gatgagggag
     1321 gcgagctgac gcgccggcca gcggtcctta tcgaggcctt cggcttcgat cacctgcttc
     1381 aataggcgga tctgatcgtc ggtatcgagg atggtgaagc cggacttgag gccgacgagc
     1441 tcggcgtggc gacgcaggat cttgacgccg atggagtgga acgtgccgag ccacggcatg
     1501 ccctcgacgg cgccgccgac gaggttgccg atgcgctcct tcatctcgcg cgccgccttg
     1561 ttggtgaagg tgacggagag aacctgtccg gggaatgccc ggcgcgtgcc aaggatgtgg
     1621 gcgatgcgcg tcgtcagcac gcgtgttttg ccagtgccgg caccggcgag aaccaagacc
     1681 ggtccgtcga gcgcttcgac ggccgcccgc tgctcgggat tgaggtcgtc gagatagggc
     1741 ggagagggca ccgcggccga gccctgcagc gcgcgcgccg aaatgggccg ccgtggcgcc
     1801 tcgggtgcgg cggcgggcaa gtcgaacggc ggatcatcgg ccgcagccat aggcgccgcc
     1861 ttggacagcg gcgctgcggc cggcaaatca aacggtggct cgtccgcgtc gtccgatggg
     1921 gccgcgttcg ccaaatgttt tccgctttgc gccatgcttt ccgaactata tcaccgggct
     1981 tggcggagca acggttgcag ggtctccacc cacaccagat atggggtggc atatggggcg
     2041 ttgcgccctg gta
//
LOCUS       DATNAE010000248         6342 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2057608, whole genome shotgun sequence.
ACCESSION   DATNAE010000248 DATNAE010000000
VERSION     DATNAE010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6342
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2057608"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1242
                     /locus_tag="VN518_05600"
     CDS             <1..1242
                     /locus_tag="VN518_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HXE04082.1"
                     /translation="FMPWLAGTALLHSALVMEKRDALKVWTILLAIITFSLSLMGTFL
                     VRSGVLTSVHSFAVDPARGVFILAIMGIFIGGALTLFAIRAPLLSQGGLFAPISREGS
                     LVLNNLLLVTACAAVFVGTLYPLALESLTGEKISVGGPFFNYTFVPLIVPLLMAVPFG
                     PMLAWKRGDIVAAGQRLMAAAILALVVAVAVLAWQQRGPWLAPFGIGLGIWLICGALS
                     EIAFRVKLFDGSLTDAARRLGNLPRSAFGTAFAHAGLGIVVIGIVATTAWRSESVLAM
                     KPKESADIAGYQLTFEGVAPREGPNYRERVGLFKVARGGQAVTTLEPSKREFTVERSS
                     TTEAGIHDSWRGDLYVVLGDPLKDGAYSVRIYFNPLVRLIWLGAVVMFIGGGLSLSDR
                     RLRIGAPKRAKAMGQGLAPAE"
     gene            1247..1750
                     /locus_tag="VN518_05605"
     CDS             1247..1750
                     /locus_tag="VN518_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006931222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HXE04083.1"
                     /translation="MPAPRSSPRLLRHGPLFLAAFLSFFVSLATLPAKAVEPDEVLQD
                     QALEARARHISEGLRCLVCQNQSIDDSAAPLAKDLRLLVRERLKAGDTDQQIEDFVVA
                     RYGEFVLLKPRFEPHTLILWFATPVVFIVALLIVTLAYRRRSAAAQLPPLSTAEERRL
                     KRLLEDR"
     gene            1965..3548
                     /locus_tag="VN518_05610"
     CDS             1965..3548
                     /locus_tag="VN518_05610"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HXE04084.1"
                     /translation="MTHSDQSSVTKEGSRKFTRGPLAVTAAALITVGALGLALGQDMR
                     FASAEPAQTTQVPPPQTVQTPYGAAPLSFADLVQKVSPAVVSINVKGDAKVAENDLQI
                     PGLPDLPEDNPLYDFFKQFRQGQPGQGGHPARPHPTLAQGSGFFISPDGYLVTNNHVV
                     EDAEDISVTLENGDKYPATLVGADARTDVALLKVKAPQQFPYVKFSQKDPRVGDWVLA
                     VGNPFGLGGTVTAGIISAHNRDIGSGPYDYLQIDAAVNRGNSGGPSFNLDGEVIGMNT
                     AIFSPSGGNVGIAFAVPAALVKEVVGQLQEHGTVDRGWLGVVIQNVSDDIADSIGLPE
                     AKGAMVTKVAEDGPAAKQDVKAGDVIVEVNGDKIDDSRDLARKIAELHPNTDVKLAIV
                     RYGKEHQVNMKLGTFPSGKKLAALEDDKAADSGSEQLDNLGLSLAPAVKVPGAGEDGV
                     VITDVDPNSDAADKGLKAGDIILQVAGETVSQPGDVAKGVKKAMDNAKDKDKVNVLVQ
                     VKTGDQTRFIALSLKKAKV"
     gene            3625..4356
                     /locus_tag="VN518_05615"
     CDS             3625..4356
                     /locus_tag="VN518_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXE04085.1"
                     /translation="MTSLVSEIDPAARRSPTMRVLVIEDDQETASFLRRALKEQGHVA
                     EYAADGQTGLELARANAYDVLIVDRMLPKCDGLTVIGSLRAEEIRTPALILSALGEVD
                     DRVKGLRAGGDDYLTKPYAFSELLARIEALARRTSPEEGSTRFVVGDLVLDRLSHKVT
                     RAGEPIMLQPREFRLLEYLMKHAGQVVTRTMLLENVWDYHFDPQTNVIDVHVSRLRSK
                     IDKNFATPLLHTVRGAGYTIRDSAH"
     gene            4340..5797
                     /locus_tag="VN518_05620"
     CDS             4340..5797
                     /locus_tag="VN518_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HXE04086.1"
                     /translation="MTALTKLFRTTTFRLSLTYLALFGAAAVVAIFYIYWNTTVLLTR
                     QLHDTIDAEIKGLAEQYTDGGLTQLVRTVADRSQTPGNSLYFVADATGRRVAGNLTSI
                     SQELLDSIGPVEFEYSRPAEGGIETRLAFANVFRLPGGYRLIVGRDIEDRRELARVIR
                     SAMLWGLGVMALFGIGGGYLVSRNLLARIDAVAATTRTIMEGDLTGRLPVNGSGDELD
                     RLSESLNLMLARIEQLMAGLREVSDNIAHDLKTPLNRLRNRVEAALHEPYGEPAYREA
                     LEHTIEEADGLIKTFNALLSIARLEAGAGGDNKESLDVAALVRDVAELYEPVAEERGI
                     VLKAEPVEPVFVRADRQLLGQAIANLIDNAIKYGAPDRAQGGNGSEPEVSVTTEARGP
                     MAEIVVTDRGPGVPSSDRERVLGRFVRLEASRSEPGSGLGLSLVSAVARLHGGVVRLE
                     DNSPGLKVVLSLPAEGQALLNGAAHQAPGAELRPA"
     gene            5794..>6342
                     /locus_tag="VN518_05625"
     CDS             5794..>6342
                     /locus_tag="VN518_05625"
                     /inference="COORDINATES: protein motif:HMM:NF015655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamine synthetase]
                     adenylyltransferase/[glutamine
                     synthetase]-adenylyl-L-tyrosine phosphorylase"
                     /protein_id="HXE04087.1"
                     /translation="MTAFFKRIAERPAIYDAARGADILDTLTKALGADERFAPAAALL
                     RDDPKVRDLLAGALSGAAYLAALAQRDPGLLADCLTRDPDRHLEEAGADLSAAAEAAK
                     SPKEIMAALRRYKRRSALLVGLADLGGVWPTEGTLRAMSVAADTAVDQAVKFLFRRAR
                     AAGQIVNPSPTAPRGYFVIAMGK"
BASE COUNT         1086 a         2080 c         2025 g         1151 t
ORIGIN      
        1 ttcatgccct ggctcgccgg caccgcgctc ctccattcgg ctctggtgat ggagaaacgc
       61 gatgcgctga aggtgtggac catcctgctc gccatcatca ccttctcttt gagcttgatg
      121 ggcaccttcc tcgtccgctc cggcgtgctc acctcggtgc actcctttgc cgtcgatccg
      181 gcccgcggcg tcttcatcct cgctatcatg ggcatcttca tcggcggggc gctgacgctg
      241 ttcgccatcc gggcgccgct gttatcgcaa ggcggcctgt ttgcgccgat cagccgggaa
      301 gggagccttg tcctcaacaa tctgctgctc gtcacggctt gcgctgccgt gttcgtcggc
      361 acgctctatc cgctcgcgct tgagtcgctg accggcgaga agatttcggt tggcggacca
      421 ttcttcaatt acaccttcgt gccgctcatc gtgccgttgc tgatggcggt tccgtttggg
      481 ccgatgctcg cgtggaagag gggagacatt gtcgccgccg gtcaacgcct catggccgcg
      541 gcaattttgg cgctcgtcgt cgccgtggcg gtgcttgcct ggcagcagcg cggaccctgg
      601 cttgcgccct tcggcatcgg gcttgggatt tggctgatct gcggcgcgct gtcggagatc
      661 gccttcaggg tgaagctgtt cgatggctcc ctgaccgatg ccgcacgccg tctcggcaat
      721 ttgccacgtt cggccttcgg cacggcgttt gcccatgccg ggctcggcat cgtggtgatc
      781 ggcatcgtcg cgaccacggc gtggcggagt gaaagcgtcc tcgcgatgaa gccgaaggag
      841 tccgccgaca tcgccggcta ccaattgacc ttcgagggcg tggccccgcg cgaggggccg
      901 aactaccgtg agcgcgtggg cctgttcaag gtggcgcgag gcgggcaagc ggtgacgacg
      961 cttgagccgt ccaagcgcga attcaccgtg gagcgaagca gcaccaccga ggccggcatt
     1021 catgacagct ggcgcggcga cctttacgtc gtccttggcg atcccctgaa ggacggcgcc
     1081 tacagcgtca ggatctattt caatccactg gtgcgcctga tctggctcgg cgccgtggtg
     1141 atgttcatcg gcggcggctt gtcgctgtca gaccggcgct tgaggatcgg ggcgccaaag
     1201 cgggcaaagg ccatgggcca gggcctcgcc cccgccgaat agagcgatgc cggcaccccg
     1261 ctcttcaccg cgtttgcttc gccatggccc tctctttctt gcggccttcc tttcattctt
     1321 cgtgagcttg gcgacgctcc cggcgaaagc agtggagccc gacgaggttc tacaagacca
     1381 ggcactcgag gcgagagccc gccacatctc cgaaggcttg cgttgcctcg tctgccagaa
     1441 ccagtcgatc gacgattccg ctgccccgct cgccaaggat cttcgcctgt tggtgcggga
     1501 gcggctcaag gccggcgaca ccgaccagca gatcgaggat ttcgtcgttg cccgttatgg
     1561 cgaattcgtg ctgctcaagc cgcgcttcga acctcacacc ctcatactgt ggtttgccac
     1621 gccggtggtc ttcatcgtcg cgctgctgat cgtcactctg gcctatcggc ggcggtctgc
     1681 tgccgcgcag ctcccgccgt tgagcacggc agaggaacgg cggctcaagc gcttgctcga
     1741 ggatcgctga ttgccgagag ccaatcggcg caccatattt cgcctgcggg ccggcggctg
     1801 agcctggcct tgtgctccgg caaaacgcca accttaccga aatttaattc cctcgaaacg
     1861 gcggcgtaat tgccggaccc ctatattttg cgcctagagg cctcgttcga tgaggatgcg
     1921 gggctccttt agcggattct ttctatcgtg ggagaggtga ccttatgact cattctgacc
     1981 agagctccgt gaccaaagag ggctcgcgga agtttacgcg tgggccgttg gcggtaacgg
     2041 ctgccgcgct gattacggtt ggcgccttgg ggctcgcgct cggccaagac atgcgcttcg
     2101 cctcagccga gccggcgcag accacccagg ttcctccgcc gcagacggtg cagactccgt
     2161 atggagccgc acccttgagc ttcgccgacc tcgtgcagaa ggtgagcccg gcggtcgtca
     2221 gcatcaacgt caagggcgac gccaaggtcg ccgagaacga cctgcagatt cctggactac
     2281 ccgacctgcc cgaggacaac ccgctctacg atttcttcaa gcagttccgc cagggccaac
     2341 ccggccaggg cggccacccg gcacgtccgc acccgacgct cgcgcaaggc tcaggcttct
     2401 tcatttcgcc tgacgggtac ctcgtcacca acaaccatgt ggtcgaggat gccgaggaca
     2461 tttccgtgac gctcgagaac ggcgacaaat atccggccac gctggtcggc gccgacgcgc
     2521 ggacggacgt cgctttgctc aaggtcaagg ccccgcagca gttcccttac gtgaagtttt
     2581 cccaaaagga tccgcgcgtg ggtgactggg tgctcgcggt cggcaatccg ttcggcctcg
     2641 gcggtacggt gaccgcaggc atcatctcgg cccataaccg tgacatcgga tcgggcccgt
     2701 atgactatct gcagatcgac gctgcggtga atcgcggcaa ttccggcggc ccgtccttca
     2761 atctggacgg cgaggtcatc ggcatgaata ccgccatctt ctccccctcc ggcggcaatg
     2821 tcggcatcgc cttcgccgtg ccggcagcgc tcgtcaagga ggtggtcggt caactgcaag
     2881 agcatggcac cgtcgaccgc ggctggctcg gcgtcgtcat ccagaacgtg agcgacgaca
     2941 tcgccgactc gatcggcctg cctgaggcca agggcgccat ggtcaccaag gtggccgagg
     3001 atggccctgc cgccaagcag gacgtcaagg ccggcgacgt gattgtcgaa gtcaacggcg
     3061 acaagatcga cgactctcgt gaccttgccc gcaagatcgc cgagctccat cccaacaccg
     3121 acgtgaagct cgccatcgtt cgctatggca aggagcacca ggtgaacatg aagctcggca
     3181 cgttcccgag cggcaagaag cttgccgcac tcgaggacga caaggccgcc gatagcggca
     3241 gcgagcagct ggacaatctc ggcctgtcgc tggctcctgc cgtcaaggtg ccaggcgccg
     3301 gcgaggatgg cgtggtgatc accgacgtcg atcccaacag cgacgctgcc gataagggcc
     3361 tcaaggccgg cgacatcatt cttcaggttg cgggcgagac ggtgtcccag ccgggagacg
     3421 tcgccaaggg cgtgaagaag gcaatggaca acgccaagga caaggacaag gtcaacgtcc
     3481 tggttcaggt aaaaaccggc gaccagacgc gcttcattgc gctttccctg aagaaggcta
     3541 aggtttaacg gccttctacg ccggagggcg gcgcggttcc ccccgccgcg ccgcccaccc
     3601 tcgttatccc ttcgcgagga agccatgacc tcgctcgtga gtgaaataga ccccgccgct
     3661 aggagatctc caaccatgcg cgtgctggtg atcgaggacg atcaggagac ggcaagcttc
     3721 ctgcggcgtg ctctgaaaga gcagggccat gtcgccgaat acgcggcgga cggccagacc
     3781 gggctcgagc tcgcgcgagc caacgcctat gacgtgctca tcgtcgaccg gatgctgccg
     3841 aaatgcgatg ggcttaccgt gatcggctcg ctccgcgccg aggagatcag aacgcccgcg
     3901 ttaatcttaa gcgcgcttgg cgaggtcgac gaccgggtca agggcctgcg cgccggcggc
     3961 gacgactatc tcaccaagcc ttatgccttc agcgagttgc tcgcccgcat cgaggcgctc
     4021 gccaggcgca cctcgccgga ggagggaagc acgcgcttcg tggtcggcga tctcgtgctc
     4081 gaccgtctct cgcacaaggt gacgcgcgcc ggcgaaccga ttatgttgca gccgcgcgag
     4141 tttcgcttac ttgaatatct catgaagcat gcgggccagg tggtgacccg gaccatgttg
     4201 ctcgagaacg tatgggacta tcacttcgat ccgcagacca acgtcatcga cgttcacgtc
     4261 tcgcggcttc gctcgaagat cgacaagaac tttgcgacgc cgctgctgca caccgtccgc
     4321 ggcgccggct acaccatccg tgacagcgct cactaagctt ttccgcacca cgacctttcg
     4381 tctgtcgctg acctatcttg cgctgttcgg cgcggcggcg gtcgtggcca ttttctacat
     4441 ctattggaac accacagtgc tgctgaccag gcagctgcac gacaccatcg acgccgagat
     4501 caaaggtctt gccgagcaat acaccgacgg cggactgacc cagctcgtgc gcaccgtggc
     4561 cgaccgcagc cagacgccgg gcaacagcct ctatttcgtc gccgacgcca ccgggcgccg
     4621 cgtcgctggc aacctcacca gcatctccca ggaacttctc gacagtatcg gaccggtcga
     4681 attcgaatat agccgcccgg cggaaggcgg catcgaaacg aggctcgcct ttgccaatgt
     4741 gttcaggctg cccggaggct accgcctcat cgtcggccgc gacatcgagg accgccgcga
     4801 gctcgcgcgc gtgatccgct cggccatgct gtggggactt ggcgtgatgg cgctgttcgg
     4861 catcggcggg ggctatttgg tcagccgcaa cctgctcgcc aggatcgatg cggtcgccgc
     4921 caccacgcgc accatcatgg agggcgatct caccgggcgc ctgccggtga acggctcggg
     4981 cgacgagctt gaccgcctgt cggagagcct caaccttatg ctggcgcgga tcgagcagct
     5041 tatggccgga ttgcgcgagg tctccgacaa tatcgcgcac gatctcaaga cgccgctcaa
     5101 ccgcctgcgc aaccgcgtgg aggcggcgtt gcatgagcct tatggcgagc ccgcctatcg
     5161 cgaagcgctg gagcatacga ttgaggaggc cgacgggctg atcaagactt tcaacgcgtt
     5221 gctctcgatc gcgcggttgg aagctggcgc cggcggcgac aacaaagaga gcctcgacgt
     5281 tgcggcactc gtccgcgacg tggcggagct ttacgagccg gtggcggagg agcgcggcat
     5341 tgtgctgaaa gccgagccgg tcgagccggt gttcgtgcgc gccgatcgtc aattgctcgg
     5401 ccaggccatt gccaatctca tcgacaatgc gatcaaatac ggtgcgcctg accgagccca
     5461 aggcggcaac ggatcggagc cggaagtgag cgtgaccacc gaggcgaggg gacccatggc
     5521 ggaaatcgtc gtcaccgatc gcgggcctgg cgtgccgagc tcagaccgcg agcgcgtgct
     5581 tggacgcttc gtcaggctcg aggcaagccg gtccgagccg gggagcggcc tgggcctcag
     5641 cctcgtctct gccgtggcgc gactgcatgg tggcgtggtc aggctcgaag acaacagccc
     5701 cgggctcaag gtggtgctgt ccttgccggc ggagggccag gctctgctca acggagccgc
     5761 gcatcaggcg cctggagcag agttaaggcc ggcgtgacgg cgttctttaa gcgcatcgcc
     5821 gagcgtccgg cgatttatga cgcggcgcgc ggcgctgaca tcctcgacac attgacgaaa
     5881 gcgcttggcg cggacgagag attcgctccc gccgccgctc ttttgcggga cgacccgaag
     5941 gttcgcgacc tgctcgcggg cgccctgtcg ggcgcagctt atctcgccgc gctcgcccag
     6001 cgcgacccgg ggcttctggc agattgcctc acgcgcgatc ccgaccgcca tctcgaagag
     6061 gccggcgccg acctcagcgc cgcggccgaa gccgcgaagt cgccgaagga gatcatggca
     6121 gcgctccgcc gctacaagag gcggagcgcg ctactcgtgg ggcttgccga tcttggcggg
     6181 gtctggccga cggaagggac gctgcgcgcc atgagcgtcg ccgcggatac cgcggtcgat
     6241 caggcggtca agtttctctt ccgccgggca cgcgccgccg gccagatcgt caacccttcg
     6301 cccaccgcgc cacgcggcta tttcgtcatt gccatgggca ag
//
LOCUS       DATNAE010000249         3650 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_551711, whole genome shotgun sequence.
ACCESSION   DATNAE010000249 DATNAE010000000
VERSION     DATNAE010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3650
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551711"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..882
                     /locus_tag="VN518_05630"
     CDS             <1..882
                     /locus_tag="VN518_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010970660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HXE04088.1"
                     /translation="YTDALPTYIHPETGRVHTSYALAATTTGRLSSLEPNLQNIPVRT
                     SEGRAIRKAFVSDKGKRLISADYSQIELRVLAHMADTPTLRQAFADGLDIHAMTASEM
                     FGVPIEGMDPAIRRRAKAINFGIVYGISAVGLAAQLGIGRSEAGAYIKTYFERFPGIR
                     DYMEATKAEARRQGYVKTLFGRKVHYPEINTKNPSLRGNFERAAINAPIQGSAADIIR
                     RAMIRMVPALAEAGLNARMLLQVHDELVFEAPEDEVDKTMEVAQSVMEKAPEPVLKLR
                     VPLKVDARAGDNWEAAH"
     gene            1020..1253
                     /locus_tag="VN518_05635"
     CDS             1020..1253
                     /locus_tag="VN518_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE04089.1"
                     /translation="MGKCAQCGNNYDQSFEVTMGGRTLTFDSFECAIAALAPVCPHCG
                     VRIVGHGVQHGDTIYCCVHCAKHEGVKGLVDRD"
     gene            complement(1326..1580)
                     /locus_tag="VN518_05640"
     CDS             complement(1326..1580)
                     /locus_tag="VN518_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE04090.1"
                     /translation="MVNIYPFIELIRAASAPDVAEHIKGLSLVNCKFANVVVLSYDKL
                     VAQLDCSTPEDGTKVVLEELPKIEGVVQTNVIAVVKPVQH"
     gene            1818..2300
                     /locus_tag="VN518_05645"
     CDS             1818..2300
                     /locus_tag="VN518_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE04091.1"
                     /translation="MGQCLLALGLVLLATPALALPAPMSEQELTDKSDLIATVRVLSV
                     SCTVVAKDDKTGEDLPFYLARLRLLEVKKGDAKKGDEILVTWRAVPKGIVGPWTVNYY
                     PGEVVLTHLVKKTGGVSYGSTWWNAKGEDVKAPDNSELPTTPGETFDAPKESTDQHPL
                     "
     gene            complement(2311..3042)
                     /locus_tag="VN518_05650"
     CDS             complement(2311..3042)
                     /locus_tag="VN518_05650"
                     /inference="COORDINATES: protein motif:HMM:NF005458.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HXE04092.1"
                     /translation="MKTAIAISHVCFEDAGTLEDAITARGLSFRYLQAGVDDLSPAKD
                     ADILVVLGAPIGVYEVDRYPFLKEDFAVIEAALARGKPIIGICLGSQLLAAVLGARVY
                     PNREKEIGWGLLGLTPDGRKSPLAALSENKFRVLHWHGDTFDLPRGATWLAETAITPH
                     QAFSANNGKVLALQFHVEQHTRGMERWLVGHTLELTTAGVDVNQLRADTQRLGPGMEK
                     ASRTMFDLWLDHVGVAPARATAPAK"
     gene            complement(3171..>3650)
                     /locus_tag="VN518_05655"
     CDS             complement(3171..>3650)
                     /locus_tag="VN518_05655"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HXE04093.1"
                     /translation="EGNGSIVGRFGFMLNNMLGEEDLSPEGLGKWRQGVRLSSMMAPT
                     VMLQPNGTVIALGSGGSNRIRTAILQVAVNLLDRGMSLEDAVEAPRLHLERDGKLSFE
                     PGLPEAVESAFRALGERAHAWPAPNLFFGGVHAARRFSDGGVEGAGDPRRGGVAIVV"
BASE COUNT          682 a         1181 c         1155 g          632 t
ORIGIN      
        1 tataccgacg cattgcccac ctatatccac cccgagactg gccgcgtgca cacttcctac
       61 gccctcgccg ccaccactac gggccggctc tcgtcgctcg agccgaattt gcagaatatt
      121 ccggtgcgca cgagcgaagg ccgcgccatt cgcaaggcct tcgtgtcaga caagggcaag
      181 cggctgatct cggccgatta cagccagatc gagcttcgcg tgctcgccca tatggccgac
      241 acgccgaccc tgcgccaggc cttcgccgac gggctcgaca ttcacgccat gacggcttca
      301 gagatgttcg gcgtgccgat cgaaggtatg gacccggcca tcaggcgccg cgccaaggcg
      361 atcaatttcg gcatcgtcta tggtatctcg gccgttgggc tcgccgccca gctcggcatc
      421 ggcagaagcg aggccggcgc ctacatcaaa acctatttcg agcgcttccc cggcatccgc
      481 gactacatgg aggcgaccaa ggccgaggcg cggcgacagg gctatgtgaa gaccctgttc
      541 gggcgaaagg tgcattaccc agagatcaac accaagaacc cgtcactccg cggcaatttc
      601 gagcgcgccg ccatcaacgc gccgattcaa gggagcgccg ccgatatcat ccgccgcgcc
      661 atgatccgca tggtgccggc gctcgccgaa gcgggcctca acgcgcgcat gctgctgcag
      721 gtacatgacg agctcgtgtt cgaggcgcct gaggatgagg tcgacaagac catggaggtg
      781 gcgcagtccg tgatggagaa ggcgccagag ccggttctga agctaagggt gccgctgaag
      841 gtcgacgccc gcgccggcga caactgggaa gccgcgcatt agagtttgca gtgggcgcgt
      901 catcccggcg aaagccggga tccagtcctt tttatttgcg cttcgcgcgc tggattccgg
      961 cttgcgccgg aatgacgaag cgactgctat gtttcccgcg tcaatcgagg gaacacgcca
     1021 tgggcaaatg cgcgcaatgc ggcaacaact atgaccagag cttcgaggtc accatgggcg
     1081 gacgcacgct caccttcgac tcgttcgaat gcgccatcgc cgcgctcgcg ccggtctgcc
     1141 cgcattgcgg cgtacgcatc gtcggccacg gcgtgcagca cggcgacacc atctattgct
     1201 gcgtccattg cgccaagcat gaaggcgtga agggcctcgt cgaccgggat tgagggtgta
     1261 gggtgtcaga gcgaagcgtg cccaccatcc gcgtggcaac ggcgaaaccg ttgcccaccc
     1321 tacgatcaat gctgcaccgg cttcaccacg gcgataacgt tggtctgcac cacgccctcg
     1381 atcttgggca attcctcaag caccacctta gtgccgtcct ctggcgtcga gcagtcgagc
     1441 tgcgccacga gcttgtcgta ggacaacacc accacgttgg cgaatttgca gttgacgagg
     1501 ctgagccctt tgatgtgctc ggcgacatcg ggggcgctcg cggcgcggat cagctcgatg
     1561 aagggataga tgttcaccat gggtcgttcc tcgcgttggc taattgtcag aagaaagcat
     1621 aggcagacga catagtttcg tcattgccgg gcttgttccc cggatcaagt ccggggacag
     1681 gctccggcaa tccaataggg cgccaaagtg aaaccccttc cgctgcaaaa tgtggtaggg
     1741 cctcactgga tgcccgggac aagcccgggc atcacgcgga aacaggaatt tcgttggaga
     1801 actcgatgat tgcacgcgtg ggacaatgtc ttcttgctct tggcttggtt ctcctcgcca
     1861 cgccggcgct tgcgctcccg gcgccgatgt ccgaacagga gctgacggac aagagcgacc
     1921 tcatcgccac cgtccgcgtg ctgtcggtga gctgcaccgt cgtggccaag gacgacaaga
     1981 ccggagaaga tctgccgttc tatctcgcga ggctcaggct gctcgaggtg aagaagggcg
     2041 acgccaagaa gggcgacgag atcctggtga cctggcgggc ggtacccaag ggcatcgtcg
     2101 ggccgtggac cgtcaactac tatcccggcg aggtggtgct gacccatctc gtcaagaaga
     2161 ccggcggggt gagctacggc tccacctggt ggaacgccaa gggcgaggac gtcaaggcgc
     2221 ccgacaacag cgagctgccg acgacgccgg gcgaaacctt cgacgcgccg aaggagtcca
     2281 ccgaccagca cccgttgtga tgcggtaagc ctacttcgcc ggcgccgttg cccgcgcggg
     2341 cgcaacgccg acatggtcga gccacaggtc gaacatcgtc cggctcgcct tctccatgcc
     2401 aggaccaagg cgctgcgtat cggcgcggag ctggttgaca tcgacgcctg cggtagtgag
     2461 ctcgagcgta tggccgacga gccagcgctc catgcctctc gtgtgctgct caacgtggaa
     2521 ttgcagggcg agcaccttgc cgttgttggc actgaaggcc tgatgcgggg tgatcgcggt
     2581 ctcggcgagc caggtcgcgc cgcgcggcag gtcgaaggtg tcgccgtgcc agtgcagcac
     2641 cctgaacttg ttctccgaga gcgccgcgag cggcgatttg cgcccgtcag gggtcaagcc
     2701 cagcaggccc cagccgatct ccttctcgcg gttgggatag acccgggcgc cgagcacggc
     2761 ggcgaggagc tgcgagccga ggcagatgcc gatgatcggc ttgccgcggg cgagcgccgc
     2821 ctcgatcacc gcgaagtcct ccttcagaaa cggatagcgg tcgacctcgt agacgccgat
     2881 gggcgcgccg agcaccacga gaatatcggc atccttggcc ggtgacagat cgtcgacgcc
     2941 ggcttgcagg taacggaagg aaagcccgcg cgctgtaatc gcgtcttcca gcgttccggc
     3001 atcctcgaag cacacgtgac taatggcgat ggcggttttc atgcttgcct cttagccttt
     3061 gggtcggcgc ctgatcaatg ttgcgcctga cgagcgatcc ttgttgacaa ttagcgacgc
     3121 ccgatctgca gtttacgaca taggcagatc accgccacac gtcttgcagc tcacaccaca
     3181 attgcgacgc cgccgcgacg cggatcgcct gcgccctcca cgccgccgtc gctgaagcgg
     3241 cgcgccgcgt gcacgccgcc gaagaacagg ttcggcgcgg gccaggcatg ggctctctcg
     3301 ccaagcgcgc ggaaggcgct ttccacggcc tctggcaagc ccggctcgaa gctgagcttg
     3361 ccgtccctct ctagatgcag ccgcggcgcc tccaccgcgt cttccaggct catgccgcgg
     3421 tcaagcagat tgacggcgac ctgcaggatg gcggtgcgga tgcggttcga tcctccgctg
     3481 ccgagggcaa tcacggtgcc gttgggctgc aacatgacgg tcggcgccat catcgaggag
     3541 aggcggacgc cttgccgcca tttccccagg ccctcaggcg acagatcctc ctcgccgagc
     3601 atgttgttca gcatgaagcc gaacctgccg acgatcgagc cgttgccctc
//
LOCUS       DATNAE010000250         3401 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_852868, whole genome shotgun sequence.
ACCESSION   DATNAE010000250 DATNAE010000000
VERSION     DATNAE010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_852868"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="VN518_05660"
     CDS             complement(<1..600)
                     /locus_tag="VN518_05660"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HXE04094.1"
                     /translation="MLRLWALLMGLVVAAPALADQRVALVIGNDAYSSVPVLQKAAAD
                     ARTMAASLSKLGFAVDSGVNISRTETNRKLQDFLNRIEPGDVALFYFAGHGVEIAGAN
                     YLLPIDIPEATPGNEEFLKSEAISLNAVLDGLRERKARVTILVIDACRDNPFAIKTGR
                     TVGGARGLALVVPPQGTFIMYSAGAGEAALDRLSDADPEP"
     gene            complement(970..1335)
                     /locus_tag="VN518_05665"
     CDS             complement(970..1335)
                     /locus_tag="VN518_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE04095.1"
                     /translation="MPSTTDTDSIILGVAAFLFAFALIALIAWAFKTFVLTGRSGPGL
                     LRGRDRRLGVVETASVDGHRKLILIRRDTVEHLIMTGGPVDVLIETGIAGQQPLGPPL
                     EEVIIARAETRPAPDFSKT"
     gene            1611..>3401
                     /locus_tag="VN518_05670"
     CDS             1611..>3401
                     /locus_tag="VN518_05670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HXE04096.1"
                     /translation="MTDIDAPSRYAEPAADQSGRGGNVALVVALALALAVAAVGLAMI
                     SREMAEPFVLAILGGLAVVGVFCLFAGAVGILHFGQTQARNDVTKAFVDNLPQGALIA
                     DQSGRVLYANESYRNLLGLEPDMVVLAPDRAFAGNSFLAEQIFRLSRAAQQGRALREE
                     FRLPPLGEAEEAPGVRPRWFRISVQPMPSEPDSGRKGGLVVWQVDEVTADRERQEAGF
                     EKVQQAIAYLDNAPAGFFTADGEGTIDYLNATLAQWLGLDLADAASGQLKLANIMSED
                     GAALIARAGRGAAHGDTRRFDIDLVKADGTTLPVRILHCLPRYGGNGGLAHALVLNRA
                     PGGAEEAAAAELRFARLFNSAPIAIATVDREGIISTTNAAFVRLFGRAVDGVPARKVT
                     LADLADADGAEALRKALDAAIAGQGLIEPVDIAFEGDKERSGRVYLSPVERAEGESEA
                     AIAYAIDTTQQRALEMQIAQSQKMQAIGQLAGGIAHDFNNMLTAIIGFSDFLLMNHRP
                     TDPAFQDIMNIKQNANRAAGLVRQLLAFSRQQTLRPERLQLGDVLSELSILLGRLLGE
                     NIELRLDSGSDIWPVKADLHQFEQVIINLAV"
BASE COUNT          609 a         1131 c         1086 g          575 t
ORIGIN      
        1 aggctcggga tcagcgtcgc tcaggcggtc gagcgcggct tcgccggccc ccgccgaata
       61 catgatgaag gtcccttgcg ggggtaccac cagtgcgagc ccgcgcgcgc cgccgacggt
      121 gcggccagtc ttgatggcga agggattgtc gcggcaggca tcaatcacga ggatggtgac
      181 ccgggccttt cgctcgcgca gaccgtcgag cacagcgttg agcgaaatcg cctccgattt
      241 gaggaattcc tcgttgccgg gtgtggcttc gggaatgtcg attggaagga ggtagttcgc
      301 cccggcgatc tcgacgccat ggcccgcgaa atagaacagc gccacgtcgc caggctcgat
      361 gcgattcaag aagtcctgca attttcgatt ggtttctgtg cgggagatgt tcacgcccga
      421 gtcgactgcg aagccgagct tggaaagcga ggcggccatg gtgcgcgcat cggcggcggc
      481 cttctgcagc accggcacgc tgctgtaggc gtcgttgccg atgacgagcg cgacgcgctg
      541 atcagcaaga gcgggcgcgg cgaccacgag gcccatcaaa agcgcccaga gacgaagcat
      601 gaagcagcct tcaccaccag acttgacttg acgacgctca agctaccgcg actggcgtga
      661 agcgcggaca aaaaaactcc ccgcttttgg gggcggggag tggagtgttt gggggactga
      721 catgactggc cgaagccagt cggtgtcgga ccattagcag ggaactgccg gcgtccttac
      781 ggcatgagcg ctgaatctac cggcgtcact tcatcgcctg ccacaactac acatggcggc
      841 cgtaagttcg agtgtcctgt ctagcgcaac ttggctctgg cgacaagcga aaccaccgtt
      901 gccagagtgt cacggtcaag cacaaatttt gccatttagc ggagccgaat cggcggtttt
      961 ccgcgcttct caggtcttgc tgaagtcagg cgcaggacgc gtctctgccc tggcgatgat
     1021 cacctcctcg agcggcggcc cgagcggctg ttgtcccgcg attcccgtct cgatcagcac
     1081 gtcgaccgga ccgccggtca tgatcagatg ctccaccgtg tcgcggcgga tgaggatgag
     1141 cttgcggtgt ccatcgaccg atgccgtctc caccacgccg agccgtcgat cgcgcccgcg
     1201 taagaggccc ggaccgctcc gcccagtgag cacgaaggtc ttgaacgccc aggcgatgag
     1261 cgcgatgagc gcaaacgcga acaggaaggc ggccacgcca agaatgatgc tgtcggtgtc
     1321 cgtcgtcgag ggcatttcag ggtcctgaac gtcagcggcc ggaggctcgc cgccaaggct
     1381 tgtttgccgg cccctaataa aaccgttaac caaggtacca atatgacgcc ggaaggctcc
     1441 cttgcccgcc agcgcggcgg tcctcgatgc actgcaaggc aggcccggcg aggccacaat
     1501 ccgcaaggaa aatggccggt tctgtcatgg atttgcgccg gcaagctgca acaacgcgcc
     1561 acatcacaat gtccggcgcg aaccgaaatc gaatcgggta gtttggaggc atgaccgaca
     1621 tcgacgcgcc ttcgcgttac gccgagccag ccgcagatca gtccggccgc ggcggcaatg
     1681 tcgctctcgt ggtcgcgctc gcgcttgctc tcgccgtggc cgccgtcggc ctcgccatga
     1741 tctcgcgcga gatggccgag cccttcgtgc ttgccatcct tggcggcctt gccgtggtcg
     1801 gcgtgttctg cctgttcgcc ggcgccgtcg gcatcctgca tttcggccag acgcaagcgc
     1861 gcaacgacgt caccaaagcc ttcgtcgaca accttccgca aggggcgctc atcgccgacc
     1921 agagcggccg cgtgctctac gcgaacgaat cctatcgcaa ccttctcggg cttgagccgg
     1981 acatggtcgt gctggcgccc gaccgtgcct ttgccggcaa cagttttctc gccgagcaga
     2041 tcttccgcct gtcgcgcgcc gcccagcagg gcagggcgct cagagaggaa tttcgcctgc
     2101 cgccgcttgg cgaggccgag gaagcgccgg gtgtgcgccc gcgctggttc cgcatctcgg
     2161 tgcagccaat gccgtccgag cccgactcag gccgcaaagg cgggctcgtg gtctggcaag
     2221 tcgacgaggt gaccgccgac cgcgagcggc aggaggccgg cttcgagaag gtgcagcagg
     2281 ccatcgccta tctcgacaat gcgcctgccg gtttcttcac cgccgatggc gagggcacca
     2341 tcgactatct caacgccacg ctcgcccagt ggctcggcct cgaccttgcc gacgcagcga
     2401 gcggccagct caagctcgcc aacatcatgt cggaagacgg cgccgcgctc atcgccagag
     2461 ccggccgcgg cgctgcgcac ggcgacacgc ggcgcttcga tatcgatctc gtcaaggctg
     2521 acggcaccac gcttccggtg cgcattctgc actgcctgcc gcgctatggc ggcaatggcg
     2581 ggcttgcgca cgcgctcgtg ctcaatcgcg cccctggcgg agcggaagag gccgccgccg
     2641 ccgagcttag atttgcgcgc ctgttcaact cggcgccaat cgccatcgcc acggtcgacc
     2701 gagaaggcat catcagcacc accaacgccg ccttcgtgcg tctgttcggc cgcgccgtcg
     2761 atggcgttcc ggcgcgcaag gtgacgctgg cagaccttgc cgacgccgat ggcgccgagg
     2821 ccctgcgcaa ggcgctcgat gcggccatcg ccggccaagg cctgatcgag ccggtcgaca
     2881 tcgcctttga gggcgataag gagcggagcg gccgtgtcta tctgagcccg gtcgaacggg
     2941 ccgagggcga gagcgaggcg gccatcgcct acgccatcga caccacccag cagcgtgcgc
     3001 tcgagatgca gatcgcccag agccagaaga tgcaggccat cggccagctc gccggcggca
     3061 tcgcgcacga cttcaacaac atgctgacgg cgatcatcgg cttctccgac ttcctgctga
     3121 tgaaccaccg cccgaccgat cctgcctttc aggacatcat gaacatcaag cagaacgcga
     3181 accgcgccgc tggtctggtg cggcagctcc tcgccttctc ccgccagcag accttgcggc
     3241 ctgaacgtct ccagcttggc gacgtgctgt cggagctgtc gatcctgctt ggccgcctac
     3301 tcggcgagaa catcgagctg aggctcgaca gcggcagcga catctggccg gtgaaggccg
     3361 atctgcatca gttcgagcag gtgatcatca acctcgcggt c
//
LOCUS       DATNAE010000251        10656 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_1446423, whole genome shotgun sequence.
ACCESSION   DATNAE010000251 DATNAE010000000
VERSION     DATNAE010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 10656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10656
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1446423"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..1328
                     /gene="argH"
                     /locus_tag="VN518_05675"
     CDS             3..1328
                     /gene="argH"
                     /locus_tag="VN518_05675"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HXE04097.1"
                     /translation="MDFDKRLWRHDILASKAHAVMLADAGIITKKEGEEMARGLDKIA
                     AEIEAGKFNFVRALEDIHLNVESRLAELIGPVAGKLHTARSRNDQVATDLRLYVRDEI
                     DSIDALLARLQLALAETALAHAATVMPGFTHLQTAQPVTFGHHLLAYAEMFGRDRGRF
                     ADARTRLNESPLGAAALAGTGFAIDRDATAKALGFARASANSLDAVSDRDFALESLAA
                     ASIAAIHLSRLAEEIVMWTSPQFGFARLSDKYTTGSSIMPQKRNPDAAELVRAKTGRI
                     AGAFFALLMVMKGLPLAYAKDMQEDKEPVFDALASLSLGLAAMAGMVGDLEPDAKAMK
                     KAAGRGHATATDLADWLVREAGLPFREAHHVTGRIVALADSKGCDLAKLTLEDLKSVD
                     KRLTEDAFSVLDVSKSVKSRRSYGGTAPGNVRREAKKWIKRLKKAPRSR"
     gene            1377..1562
                     /locus_tag="VN518_05680"
     CDS             1377..1562
                     /locus_tag="VN518_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HXE04098.1"
                     /translation="MGSALRVLLAIALILSVGLSACGKRGSLDRPKPSYPETKADPST
                     KMSQAPARSLWIDRLLQ"
     gene            1579..2877
                     /gene="lysA"
                     /locus_tag="VN518_05685"
     CDS             1579..2877
                     /gene="lysA"
                     /locus_tag="VN518_05685"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HXE04099.1"
                     /translation="MHHFTYRQGVGGGSILHAEDLSLADLADSVGTPFYCYSTATLER
                     HYRVFADAFEGLDAEVCYSVKANSNLAVLATLARFGAGMDVVSEGELRRVLAAGVLPE
                     KIAFSGVGKTADEMAFALEEGIFAFNVESEPELRTLSETASSLGLSARIALRVNPDID
                     AKTHHKISTGKAENKFGIPYGEALALYRLAAALPGVDPHGVHMHIGSQITDLAPFRNA
                     FALLKDLVNELRANSFAIDFVNLGGGLGVPYRSDEPAPPPPAEYGKLVRETVGDLGCK
                     LLFEPGRLIAANAGILVARVLYVKRGEPKTFIIVDAAMNDLIRPTLYEAYHEIWPVAD
                     PAPGAAMMTSDVVGPVCETGDYLALDRALPELRSGDLIAVMTAGAYGAVIASQYNSRL
                     LVPEVLVAGDRYAVTRPRPHYGDMLAAERLPDWLKPERRS"
     gene            2949..5666
                     /locus_tag="VN518_05690"
     CDS             2949..5666
                     /locus_tag="VN518_05690"
                     /inference="COORDINATES: protein motif:HMM:NF025152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02302 family protein"
                     /protein_id="HXE04100.1"
                     /translation="MMRDLFARKKAPPDTPEDKSLARRFEWRVDLSRLALVGERVWEA
                     LLWPFLVLAAFLILSMFDLWSALPPLLHRALLVAFAVALLVSLVPLIRFPMPTRAEAL
                     RRLERNADIKHRPASSYEDKLGTTSRADTALLWAAHRERLSRLVAKLKPSWPLPRTDR
                     KDPYAVRSALLLVLVVALLAAGPNRWDRITAAFTPAARTSAALMRLDAWVTPPVYTQV
                     APIVLADGSESVGAGNETFRALSVPERSELIVRAFAPRGEAVSLVSQPEDGSPANAVI
                     PKITGSQGLLEFKLPLASPGAVDVRVGGNTVAKWRFDLIKDGVPQIALMSNPTTTPRG
                     ALRLSFRATDDHGVASAEARFALADSEEAGGLAAQAPELDGGKQEADPLPEAPLMPLQ
                     LPKVNAKLVEGKSSQDLTAHPWAGLKVRMTLAARDQAEQTGLSQPYEFILPERKFTKP
                     LAKAVVEQRKKLVRERDSATSVAMALDALTIGGERAIPDSSVYLALRDTYWRLDNDQS
                     PQSIASVVSQLWDLALRIEDGNIPEAERDVKAAQDRLSEALKRNASPEELDRLVDELR
                     SALSRYLQALAEQAQKNGNPAPDQKSKDGEQTVSDQELDKLLNNIEQLAKSGSKELAE
                     QMLSELKDILERLQAGTSTDNAKQQRMSKMMKDLNELVSKQQKLLDETFKARREQGGN
                     RQNDAFNVSPPGQPMEFGPGIFMAPFGTPLEDGSDNGQSPGGDESAQGKSAGGGQKPG
                     EKAQGQRPGGKFDDLGRRQGELRDALQSLIDRFRIEGANPPEQFPEAGEAMGDAKEAL
                     GQSNLDRATDEQGRALDQLRQGAQSLAEQMMENGEAQTGKGQGNSGKDPLGRPDRSNR
                     PDLGLSVKVPDQIDIQRAREVLDEVRRRLGDPSRPMFELDYLERLIRSY"
     gene            complement(5663..6217)
                     /gene="hpt"
                     /locus_tag="VN518_05695"
     CDS             complement(5663..6217)
                     /gene="hpt"
                     /locus_tag="VN518_05695"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020083952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HXE04101.1"
                     /translation="MTAAGGLADGAIKPLYSAEAIALRVDQLAGDIAARRLDDLMVVA
                     VLKGSFIFAADLIRALHRHGLKPEIDFVYLASYDGKRVSRGEVRVLRDVESDIKGRNV
                     VIVDDIFDSGRTLAFAKTLLAEGGARQVLTCVLVDKQVKRASAITPDFIGFSCPPVFV
                     VGYGMDLHNRYRELPFIGELTASG"
     gene            complement(6214..7224)
                     /locus_tag="VN518_05700"
     CDS             complement(6214..7224)
                     /locus_tag="VN518_05700"
                     /inference="COORDINATES: protein motif:HMM:NF038353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HXE04102.1"
                     /translation="MTVIICPACNTRFETAAVIPPTGRKVRCSKCGNVWQAMAVIEPA
                     KPTVVSAPTPPPVQPRPTPAAPMPPRRAPEPRPAAAPRPAPPEPRPGSTGLGNAMGRF
                     PSQQGFAPKPTNGVHPNDRPPPPPKPTGPLFSTEDEMQPDVRGDGAGLTAGMGANSRA
                     GAEAEDLGSYNAGALINPDAGLATNVPIAEGRERKLPPAVAIGWGVLALLLAMLAAFV
                     ALSPKTVVSVLPGAAKLYDMMGKPVNVGLKIENVHSSWSDAGGQRVLQVDGDIVNPTA
                     SEVSVPTVVVALQDNSGKELTQATASVLSLAAGAKTSFTLQIPGAPENVSSLKVSFAK
                     PK"
     gene            7366..8022
                     /gene="ftsE"
                     /locus_tag="VN518_05705"
     CDS             7366..8022
                     /gene="ftsE"
                     /locus_tag="VN518_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HXE04103.1"
                     /translation="MIRFENVGLRYGNGPEILRDVTFHVVPGSFHFLTGPSGAGKTTL
                     LRLLFMSLRPTRGLIFVFGENVSDLSAAGRAELRRRIGIVFQDFRLLDHLTTWENVAL
                     PLRVMGKKESDYREDVTDLLRWVGLGDRIDAYPEVLSGGEKQRAAIARAVIGRPELLL
                     ADEPTGNVDPMIADRLLRLFVELNRLGTSVIIATHDYGLMDQLDAPRLVLYEGHLEIR
                     "
     gene            8019..9002
                     /locus_tag="VN518_05710"
     CDS             8019..9002
                     /locus_tag="VN518_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXE04104.1"
                     /translation="MTTVQLIADIRDYPWGNLFAGILPARLQPKTAQIVPSASIAGNA
                     LTVVIAIMSFLACLTAGAVYMVNQSAEAWVKDISSEITVELDPVNTADIEKKMTLVSL
                     FLAKQAGVTRVKPLTAEDSAKLLEPWLGHSEALSALPIPRLIAVEIDRSNPPDIKAIT
                     EALSQNFEGVTLDDHRRWRAEIRTLTRSAAAGGFAVLALVAAATVAVIVSATRSAMAS
                     NREIIEVLHFVGANERFISREFERHFVGLGVRAGLVGAISAALVFLLLPVAMHLLGGE
                     PATATEMSRLVGSGELDLKGYLLCMLVVFVIACLCMITSRLGVIRVLKTYA"
     gene            9102..9785
                     /locus_tag="VN518_05715"
     CDS             9102..9785
                     /locus_tag="VN518_05715"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HXE04105.1"
                     /translation="MTHVEPESSSAFELLVGVLRLIFDLVMLAVVMLVIGFFIFTGSI
                     ERSQLEPRTADGIAVLTGGAARIDEAMKLLTQQKAKRLLITGVNRTTSTEELKQLASQ
                     GDQLFSCCVDIDKEARNTIDNATETSQWVARNHYTSVIVVTSNYHMPRALAELARVMP
                     GVTLIPYSVVDNNVHLDRWWTFPGTTRLLISEYLKYLPALARLGATQLVSKLITGKTT
                     VAVEDPTEP"
     gene            9800..10528
                     /locus_tag="VN518_05720"
     CDS             9800..10528
                     /locus_tag="VN518_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HXE04106.1"
                     /translation="MARSLLFNVLFYVTTTLFVVIGSPLLFAPRDWAMAALKVHSNFE
                     LWLLRTIVGTKIEVRGRENIPQGPCLVASKHQSAWETFALIPIFRDPALLMKRELFFI
                     PFHGWFSRKFEMIPVDRDKGPSALRRMLREAKKRIDDGREIIIFPEGTRRPPGAPPDY
                     KTGIILLYDALGVPCLPVALNSGLFWPRRSVIRRPGTIVVEILPPIPPGLPRDEFLRR
                     LEAAIEGASSHLLVEGGAPEPARA"
BASE COUNT         1860 a         3593 c         3306 g         1897 t
ORIGIN      
        1 ccatcgactt cgacaagcgc ctctggcgcc acgatattct tgcgtccaag gcccatgctg
       61 tgatgctcgc cgatgccggc atcatcacca agaaagaggg tgaggagatg gcccgcggcc
      121 tcgacaagat cgcggccgag atcgaagccg gcaaattcaa ctttgtgcgc gcgctcgagg
      181 acattcatct gaatgtcgag tcgaggctcg ccgagctgat cggcccggtc gccggcaagc
      241 tgcacacggc gcggtcgcgc aacgatcagg tggcgaccga tctgcgcctc tatgtgcgcg
      301 acgagatcga ctcaatcgat gcgttgctcg cacgtttgca gctcgcgctc gccgagacag
      361 cgctcgctca tgccgcgact gtgatgcctg gcttcaccca tctgcagacc gcgcagccgg
      421 tcaccttcgg ccatcatctt ctcgcttatg ccgagatgtt cggccgcgac cggggacggt
      481 tcgccgatgc aagaacacgg ctcaacgaat cgcccttggg tgcggctgcg ctcgccggca
      541 ccggcttcgc catcgatcgc gacgccacgg cgaaggcgct cggctttgcg agagcgtcgg
      601 ccaattcgct cgacgcggtc tccgatcgcg acttcgcgct tgagagcctg gctgccgcgt
      661 caatcgccgc cattcatctc tcgcgtcttg ccgaagagat cgtgatgtgg acgtcgccgc
      721 aattcggctt tgcacgtctc tccgacaaat acaccaccgg ctcctcgatc atgccgcaga
      781 agcggaaccc cgacgctgcc gagctcgtgc gcgccaagac cggccgcatc gccggcgcct
      841 tcttcgctct gctcatggtg atgaagggat tgccgctcgc ttatgccaag gacatgcagg
      901 aggacaagga gccggtgttc gacgcgctcg cctcgctttc gctcgggctt gccgccatgg
      961 ccggcatggt cggcgatctc gagcccgatg cgaaggcaat gaagaaggcc gccggccgcg
     1021 gccacgccac ggccaccgac ctcgccgatt ggctggtgcg ggaggcgggg ctgcccttcc
     1081 gcgaggcgca tcacgtcacc gggcgaatcg tggcgctcgc cgacagcaag ggctgcgacc
     1141 tcgccaaact cacgcttgag gacctgaaat cggtcgataa acgactcact gaagacgcat
     1201 tttcagtgct cgatgtgagc aaatcggtca aaagcaggcg tagctatggg ggcacagccc
     1261 ctggaaatgt gcggcgggag gccaaaaaat ggataaagcg tttgaagaag gcgccccggt
     1321 cgcgataaaa aggacgtaag gaccggtcca aggtccaagg tcaaaggggc acgaccatgg
     1381 gaagcgcttt gcgcgtcctc ctggctattg cgttgatcct gtccgtaggc ttgagcgcct
     1441 gcggcaaacg cggctcgctc gaccggccga agccctcata ccccgagacc aaggccgatc
     1501 cctccaccaa gatgtcgcaa gcgcccgccc gctcgctatg gattgaccgc ctgctgcaat
     1561 aaggctttct aagcctccat gcaccatttc acttaccggc aaggagtcgg cggtgggagc
     1621 atcctgcacg ccgaggatct gagccttgcc gatctcgccg actccgtcgg cacgcccttc
     1681 tattgctatt cgacggcaac gctcgaacgg cattaccgcg tgttcgccga tgccttcgaa
     1741 ggcctggacg cggaggtctg ctattcggtc aaggccaact ccaatctcgc cgttctggca
     1801 acgctcgcaa ggttcggcgc cggcatggat gtggtgtcgg agggagagct tcgccgcgtg
     1861 ctcgccgccg gcgttctgcc tgagaagatc gccttctcag gcgtgggcaa gaccgctgac
     1921 gagatggcct tcgcgctgga agagggcatc ttcgccttca acgtcgagtc ggagcccgag
     1981 cttcgcactt tgagcgagac ggcatcgtcg ctcgggctct ctgcgcgcat cgcgctccgc
     2041 gtcaatcccg acatcgacgc caagacccat cacaagatca gcaccggcaa agctgagaac
     2101 aagttcggca ttccctacgg cgaggcgctc gcgctttatc gccttgccgc agcactcccc
     2161 ggcgtcgatc cgcacggcgt gcatatgcat atcggcagcc agatcaccga ccttgctccc
     2221 ttccgcaacg ctttcgcgct gctcaaggac ctcgtcaacg agctcagggc caacagcttc
     2281 gccatcgact tcgtcaatct cggcggcggc ctcggcgtgc cctatcgcag cgacgagcct
     2341 gccccgccgc ctcccgccga atacggcaag ctcgtgaggg agaccgtcgg cgatctcggc
     2401 tgcaagcttc tgttcgagcc agggcgcctg atcgccgcca atgccggcat cctcgtcgcc
     2461 cgcgtcctct atgtgaagcg cggagagccc aagaccttca tcatcgtcga tgcggcgatg
     2521 aacgatctga tcaggccgac gttgtatgag gcctatcacg agatttggcc ggtggcagac
     2581 cccgcgcccg gtgcagcgat gatgaccagc gacgtggtcg gcccggtctg cgagaccgga
     2641 gattatcttg cgctcgaccg ggcgctgcct gagctccgct ccggcgatct cattgccgtg
     2701 atgacggccg gagcctatgg cgccgtaatc gcctcacagt ataatagccg gcttctggtg
     2761 cctgaggtgc tggtcgccgg cgatcgctat gccgtgaccc ggccccgacc tcattacggc
     2821 gacatgctgg cggcggagcg gctgcctgat tggctcaagc ccgagcggcg ctcatagggc
     2881 ctccttttag cccgcatcgc tctccaaatt acccgcgtgc ttcacgcgtt gtgctaagcc
     2941 tgatgaagat gatgcgagat ctgtttgccc gaaagaaagc gcccccggac accccggagg
     3001 acaagtcgct cgcccgccgc ttcgaatggc gcgtggactt gagccggctt gccctcgtcg
     3061 gcgagcgtgt atgggaggcg ctgctctggc cgttcctcgt gctcgcggcc tttctcattc
     3121 tctccatgtt cgatctgtgg agcgccttgc cgcctcttct gcaccgcgcc ctgctcgtgg
     3181 ccttcgccgt ggcgctcctc gtctcgctcg tgccgctcat ccgcttccct atgccgacgc
     3241 gggccgaggc cctcaggcgt ctcgagcgca atgccgatat caagcaccgg ccggcctcct
     3301 cctacgagga caagctcggc accacatcac gcgccgacac cgcgcttctt tgggcggcgc
     3361 atcgcgagcg gctgagccgc ctggtcgcca agctgaagcc gagctggcct ctcccgcgca
     3421 ccgaccgcaa agacccttat gccgtccgtt cggcactcct gctcgtgctc gtcgtggcgc
     3481 tgctcgctgc cggtcccaac cgatgggatc gcatcactgc ggccttcacc cccgccgcgc
     3541 gcacgtcggc ggcgctgatg cgcctcgacg cctgggtgac gccgccggtc tatacccaag
     3601 tggcacccat cgttctcgcc gacggcagcg agtcggtcgg cgccggcaat gaaaccttcc
     3661 gcgcgctttc ggtccccgag cggagcgagc tgatcgtgcg cgcctttgcg ccgcgcggcg
     3721 aagccgtgag cctcgtgtcg cagcccgagg acggctctcc agcgaacgcg gtcatcccca
     3781 agatcaccgg cagccagggg ctcctggaat tcaagctgcc cctcgcctcg cccggcgcag
     3841 tcgatgtgcg ggtcggcggc aacaccgtcg ccaaatggcg cttcgacctc atcaaggacg
     3901 gcgtgccgca gattgcgctc atgagcaacc cgaccaccac gccgcgcggc gcgctccgct
     3961 tgagcttccg cgccaccgac gatcatggcg tggcgagcgc cgaggcccgc ttcgcgctgg
     4021 ccgattccga agaagccgga ggtctggcgg cgcaggctcc tgagctcgac ggcggcaagc
     4081 aagaggccga tcccctgccc gaagctccgc tcatgccgct gcaattgccc aaggtcaacg
     4141 ccaagctggt cgaaggcaag tcctcgcagg atttgaccgc ccatccctgg gccggcctca
     4201 aagtgcgcat gacccttgcc gcgcgcgatc aggccgagca gaccggcttg agccaaccct
     4261 acgaattcat cctgcccgag cgcaaattca ccaagcctct tgccaaggcg gtggtcgagc
     4321 agcgcaagaa gctcgtgcgc gagcgggatt cggcgaccag cgtcgccatg gcgctcgatg
     4381 ccttgaccat cggcggcgag cgtgcgatcc ccgacagctc ggtctatctc gccttgcgcg
     4441 acacttattg gcggctcgac aacgatcagt cgccccagtc gatcgcgagc gtggtgagcc
     4501 agctgtggga cttggcgctc cgcatcgagg acggcaacat tcccgaggcc gagcgcgacg
     4561 tgaaggcggc gcaggaccgg ttgagcgagg ctctgaagcg gaacgcttcg ccggaggagc
     4621 tcgatcgcct ggtcgatgag ctgcgcagcg cgctgtcgcg ctatctgcag gcgctcgccg
     4681 agcaggcgca gaagaacgga aatcctgctc ccgaccagaa atcgaaagac ggcgagcaga
     4741 ccgtctccga ccaggagctc gacaagcttc tcaacaatat cgagcagctc gccaagtcgg
     4801 ggtcgaagga gcttgccgag cagatgctga gcgagctcaa ggacattctc gagcggctgc
     4861 aggccggaac ctccaccgac aacgccaagc agcagcgcat gagcaagatg atgaaggacc
     4921 tgaacgagct cgtctccaag cagcagaagc tgctcgacga gacgttcaag gcgaggcgcg
     4981 agcagggcgg caaccggcag aacgacgcct tcaacgtgag cccgcctggc caaccgatgg
     5041 agttcggccc tggcatcttc atggcgccgt tcggcacgcc gctagaggat ggctcggaca
     5101 acggccagag ccccggcggc gacgaaagcg cgcaaggaaa atccgccggc ggcggccaga
     5161 aacccggcga gaaggcgcaa ggccagcgcc ctggcggcaa gttcgacgat ctcggcaggc
     5221 gacagggtga attgcgcgac gcgttgcaaa gcctgatcga ccgtttccgc atcgaaggcg
     5281 cgaacccgcc cgagcagttc ccggaagccg gcgaagccat gggcgacgcc aaggaggcgc
     5341 ttggccagtc gaatctcgac cgcgccaccg acgagcaggg ccgcgcgctc gaccagttgc
     5401 gccagggcgc tcagtcgctc gccgagcaga tgatggagaa cggcgaggcc cagaccggca
     5461 aaggccaagg caacagcgga aaggacccgc tcggacggcc cgaccgtagc aaccgcccgg
     5521 acctcgggct gagcgtcaag gttcccgacc agatcgacat tcagagggcg cgtgaagtgc
     5581 tggacgaggt tcgccgccgc cttggcgatc cgtcgcggcc gatgttcgag ctcgattatc
     5641 tcgagcgcct gatcaggtcc tattagccgg acgcggtcaa ttcgccgata aagggaagct
     5701 cgcggtagcg gttgtggaga tccatgccgt agccgaccac gaacaccggc gggcaggaga
     5761 aaccaatgaa gtcgggcgtg atcgccgaag cgcgcttcac ctgcttgtcg accagcacgc
     5821 aagtgagcac ttgcctggcg ccgccttccg cgaggagcgt cttggcgaag gccaacgtcc
     5881 gccctgagtc gaagatgtcg tcgacgatga cgacgttgcg gcctttgatg tcgctttcca
     5941 cgtcgcgcag caccctcacc tcgcctcgcg aaacccgttt cccgtcatag cttgcgaggt
     6001 agacgaaatc gatctcaggc ttaagtccgt ggcggtgcag cgccctgatg agatcggcgg
     6061 cgaagatgaa gcttcccttg agcacggcga ccaccatgag gtcgtcgagc cgccgtgcgg
     6121 caatgtcgcc ggcaagctga tcgacgcgaa gagcgatcgc ctcggcgctg tagagcggct
     6181 tgatcgcgcc gtccgcaagg ccgcccgcgg ccgtcatttg ggcttggcga agctaacctt
     6241 gaggctgctt acgttttccg gtgctccggg aatttgcagc gtgaaggagg tcttggcgcc
     6301 ggcggcgagc gagagcacac tagccgtggc ctgcgtcagc tccttgccgc tgttgtcctg
     6361 caaagccacc accacagttg gcacgctgac ctcgcttgcc gtgggattga cgatgtcgcc
     6421 atcgacttgc agcacgcgct ggcctcctgc atcgctccac gaagagtgaa cgttctcgat
     6481 tttgagcccg acgttcaccg gcttgcccat catgtcgtaa agcttggcgg cccctggcaa
     6541 caccgacacc acggtcttcg gcgagagggc gacgaaggcg gcaagcatgg cgagcaggag
     6601 cgcgagcacg ccccagccga tggcgacggc cggcggcagc ttccgctcgc ggccttcggc
     6661 gatgggcacg ttggtggcca atccggcatc gggattgatc aaggctccgg cgttgtagct
     6721 gccgagatcc tcagcttcag ctcccgcgcg cgaatttgcc cccatgcccg cggtcaatcc
     6781 ggcaccgtcg ccgcgcacat cgggctgcat ctcgtcctcg gtgctgaaca gcgggccggt
     6841 tggcttcggc ggtgggggtg gccggtcgtt cggatgcacg ccgttggtcg gcttcggcgc
     6901 aaaaccctgc tgactgggaa agcggcccat ggcgttgccc aagccggtcg aacctggacg
     6961 cggttcaggc ggagcgggac gcggcgccgc tgccggacgg ggttccggag cgcgacgcgg
     7021 cggcatcgga gccgccggcg tcggccgtgg ctggaccggg ggaggcgtcg gcgctgaaac
     7081 gacggtaggc ttggccggct cgatcaccgc catggcctgc cagacattgc cgcatttgga
     7141 gcagcgcacc ttgcggcccg tcggcgggat caccgccgcc gtctcgaagc gcgtattgca
     7201 agctggacag ataatgaccg tcatcgttca gaaccaccac cgggcaggtt tctgcggcct
     7261 cgcgtgacca gcccacgccc ttgcccacat ccattgatac cggacagcga gttccgcata
     7321 aagacgcaag ttgggccgaa tgttaagagg atcgagggcg atcgcgtgat ccgctttgaa
     7381 aatgtcgggc tgcgctacgg caatggccca gagatcctgc gcgacgtcac ctttcatgtg
     7441 gttccggggt ccttccactt cctcaccggg ccgtcaggcg cgggtaagac cacgctgttg
     7501 cgcctcctgt tcatgtcgct ccgcccgacg cgaggcttga tcttcgtttt cggcgagaac
     7561 gtgtccgacc tttccgccgc gggccgcgcc gagcttcgcc gccgcatcgg catcgtgttt
     7621 caggatttcc gtctgctcga ccatctcacc acctgggaga acgtggcgct gcccttgcga
     7681 gtgatgggca agaaggagtc ggactatcgc gaggacgtga ccgacctgtt acgctgggtc
     7741 gggcttggcg accgcatcga cgcctacccg gaggtcctct ccggcggcga gaagcagagg
     7801 gcggcaatcg cccgcgccgt gatcgggcgc ccggagctcc tgctcgccga cgagcccacc
     7861 ggcaacgtcg atcccatgat cgccgaccgc ctgttgcggc tgttcgtcga gctcaaccgc
     7921 ctcggcacct cggtgatcat cgccacccac gactacggcc tgatggatca gctcgacgcg
     7981 cctcgcctcg tgctctacga aggccatctg gagattcgtt gaccaccgtg cagctcatcg
     8041 ccgacatccg cgactatccc tggggcaatc tctttgccgg gattctgccc gcgcgcttgc
     8101 agccgaagac cgcgcagatc gtgccctcgg cttcgatcgc cggcaacgcg ctgaccgtgg
     8161 tgatcgccat catgtcgttc ctcgcctgcc tcaccgccgg cgcggtgtac atggtcaatc
     8221 aatccgccga ggcctgggtg aaggacattt ccagcgagat caccgtcgag ctcgatccgg
     8281 tgaataccgc cgacatcgag aagaagatga ccctcgtctc tctgtttctc gccaagcagg
     8341 ccggtgtcac cagggtgaaa ccgctgaccg ccgaagattc cgccaagctg ctcgagcctt
     8401 ggctcggcca tagcgaggct ctgtcggcgc tgcccatccc ccgcctgatc gcggtcgaga
     8461 tcgaccgctc caacccgccc gacatcaagg cgatcaccga ggcgctgtcg cagaacttcg
     8521 agggcgtcac cctcgacgac caccgccgct ggcgggccga gatccgcacc ctgacgcggt
     8581 cggctgctgc cggcggcttt gccgtgcttg ctctcgtcgc cgccgccacc gtcgccgtca
     8641 tcgtctcggc cacccgcagc gccatggcgt cgaaccgcga gatcatcgag gtgctgcatt
     8701 tcgtgggcgc caacgagcgt ttcatcagcc gcgagttcga gcgccatttc gtcgggctcg
     8761 gcgtccgcgc cggcctcgtc ggtgcgatca gcgcggcgct cgtgtttctg ctgctcccgg
     8821 tcgccatgca cctgctcggc ggcgagcccg ccactgccac cgagatgagc cggctcgtgg
     8881 gctccggcga gctcgatctc aagggctatc tcctctgcat gctggtggtt ttcgtgatcg
     8941 cctgcctatg catgatcacc tcgcggcttg gcgtcatccg cgtgctcaag acctacgcct
     9001 gagtgcagga aagccggaca tgttgagggc cgccaagccg atttcctatc gattcgatta
     9061 agcctattct tgcgccgcgc atggcagggc gggagtaagc ggtgacgcac gtcgagccgg
     9121 aatcttcaag cgcattcgag ctcttagtcg gcgtcctgcg cctgatcttc gatctggtga
     9181 tgctcgccgt cgtcatgctc gtcatcggct tcttcatctt caccggcagc atcgagcgca
     9241 gccagctcga gcctcgcact gccgacggca ttgccgtctt gaccgggggc gccgcccgca
     9301 tcgacgaggc gatgaagctg ttgacccagc aaaaggcgaa gcgcctgttg atcaccggcg
     9361 tcaaccgcac cacctcgacc gaggagctga agcagctcgc gagccaaggc gaccagctct
     9421 tctcctgttg cgtcgatatc gacaaggaag cgcgcaacac catcgacaat gccaccgaga
     9481 ccagccaatg ggtggcgcgc aatcactaca cgtcggtcat cgtggtgacg tcgaactatc
     9541 atatgccgcg ggcgctggcc gagcttgccc gcgtcatgcc aggcgtaacc ttgatcccct
     9601 attcggtcgt ggacaacaat gtgcatctcg accgctggtg gacctttccc ggcaccacca
     9661 gattgctgat ctccgaatat ctgaaatatc tgccggcgct ggcgcggctc ggggccaccc
     9721 aacttgtgag caaattgatc accggcaaga ccacggttgc ggtcgaggac ccaacagaac
     9781 cgtgacggcc gtcccctcca tggcgcgctc gctcctgttc aacgtgctgt tctacgtgac
     9841 caccacactg ttcgtggtga tcggcagccc gttattgttc gccccgcgag actgggcgat
     9901 ggcggcgctc aaggtgcatt cgaacttcga gctttggctg ctgcgcacca tcgtcggcac
     9961 gaagatcgag gtgcgcgggc gcgagaacat tccccaaggc ccctgcctcg tcgcctcaaa
    10021 gcatcaatcg gcctgggaga ccttcgcgct gatcccgatc ttccgcgacc ctgcgctgct
    10081 catgaagcgc gagctgttct tcatcccgtt ccacggttgg ttctcccgca agttcgagat
    10141 gattcccgtc gaccgcgaca agggcccgag cgcgcttcga cgcatgttgc gcgaagcaaa
    10201 gaagcgcatc gatgacggcc gcgagatcat catcttccct gaaggcacgc ggcggccgcc
    10261 aggcgctccg cccgactaca agaccggcat catcctgctc tacgatgccc ttggcgtgcc
    10321 ttgcctgccg gtcgccctca attcagggct gttctggcct cggcggagcg tgattcgccg
    10381 tcccggcacc atcgtcgtgg aaattcttcc gcccattccc ccgggcctcc ccagggacga
    10441 gttcctgcgc cggctggagg cggccatcga gggcgcttcc agccatttgc ttgtggaagg
    10501 aggggccccg gaacccgctc gcgcttagcc tgttaatcct tcattaactt gaacgagcca
    10561 aggttgtgtt ctaggtttgt tcttacgaat cgccgccgac cagtcttgaa ttcgtcatcg
    10621 gaaaacccat ctgacgacag ttcgagggag agggtt
//
LOCUS       DATNAE010000252         2170 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_854917, whole genome shotgun sequence.
ACCESSION   DATNAE010000252 DATNAE010000000
VERSION     DATNAE010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_854917"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1476
                     /locus_tag="VN518_05725"
     CDS             <1..1476
                     /locus_tag="VN518_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007009190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXE04107.1"
                     /translation="ALSILRLLPYPAASHPSGTILFKGENLLTLSPPALRQIRGNKIS
                     MIFQEPMTSLNPLHTIERQVGEVLKIHRGLSDRAARERVLDLLDKVGIDDPKGRLDCY
                     PHQLSGGQRQRVMIAMSLANEPDLLIADEPTTALDVTIQAQILELLSKLKAEFNMAML
                     LITHDLGIVRKMADRVCVMNNGEIVERGTMADIFAAPKHPYTKHLIASEPKGSPPAAN
                     PNAKTILEAKDLRVWFPIKRGFLRHVVGHIKAVDGIDLTVKEGQTLGVVGESGSGKTT
                     LGLALLRLISSEGPIVYLGNRIDGYDSKRMRPLRRHMQIVFQDPYGSLSPRLSIGQII
                     EEGLSIQNPTLTREERVARVDQALREVGLDPKWRDRYPHEFSGGQRQRIAIARALVLE
                     PKFLILDEPTSALDVSVQAQIVELLRELQRRHKLAYLFISHDLKVVRALANSIIVLRY
                     GKVVEQGPARTVFAKPKTDYTKALLAAAFELEATRGGAVAT"
     gene            complement(1632..>2170)
                     /locus_tag="VN518_05730"
     CDS             complement(1632..>2170)
                     /locus_tag="VN518_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012252579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HXE04108.1"
                     /translation="EPDAKSPPLALLSLNALFTAKAEEGKFLALEDGGYVFARHALPA
                     GEVVDDPVAIAEAFLGTPYLWGGRTSIGLDCSGLVQLAVEAAGHKAPRDADMQAAELG
                     EPLPWQEGTELRRGDLVFWEGHVGIMTSATDLLHANAHHMTVEIEPFAEARERIREAG
                     YEVIGVRRLPRITAAETL"
BASE COUNT          396 a          740 c          642 g          392 t
ORIGIN      
        1 gcgctctcga tcctccgtct cctcccctac cccgcggcgt cgcacccatc cggaacgatc
       61 ctattcaagg gcgagaacct gctcacgctt tccccaccgg cgctccgcca gatccgcggc
      121 aacaagattt cgatgatctt ccaggagccg atgacttcgc tcaatccgct gcacacgatc
      181 gagcggcagg tcggcgaggt gctgaagatc catcgtgggc tttccgatcg cgccgcgcgc
      241 gagcgcgtgc tcgatttgct cgataaggtc ggcatcgacg atccaaaggg ccggctcgac
      301 tgctatccgc atcaactctc tggcggccag cgtcagcgcg tgatgatcgc catgtcgctt
      361 gccaacgagc ccgacctgct catcgccgac gagcccacca ccgcgctcga cgtcaccatc
      421 caggcgcaga tcctcgagct gctgtcgaag ctcaaggccg agttcaacat ggcgatgctg
      481 ctcatcaccc atgatctcgg catcgtgcgc aagatggccg accgggtgtg cgtcatgaac
      541 aatggcgaga tcgtcgagcg cggcacgatg gccgacatct tcgccgcacc gaagcatccc
      601 tacaccaagc acctgatcgc gtccgagccg aagggctcgc cgccggccgc caaccccaac
      661 gccaagacca tcctcgaggc caaggatttg cgcgtctggt tccccatcaa gcgcggcttt
      721 ctgcgccatg tcgtcggcca tatcaaagcg gtcgacggca tcgacctcac cgtcaaggaa
      781 ggccagacgc tcggcgtcgt cggcgagtca ggctcgggta agaccacgct cgggcttgcg
      841 ctgctcaggc tgatctcaag cgagggcccg atcgtctatc tcggcaatcg tatcgacggc
      901 tatgattcaa agcgcatgcg gccgctgcga cggcatatgc agatcgtgtt ccaggaccct
      961 tacggctcgc tgtcgccacg gctttccatc ggacagatca tcgaggaggg actgtccatc
     1021 cagaacccga ctctcacccg cgaagagcgc gtggcgaggg tcgatcaggc attgcgagaa
     1081 gtcgggctcg atcccaaatg gcgcgaccgc tacccgcacg agttctccgg cggccagcgt
     1141 cagcgtatcg ccattgcccg cgccctcgtg ctcgagccga aattcctcat cctcgacgag
     1201 ccaacttcgg cgctcgacgt gtcggtgcaa gcgcagatcg tcgaactcct gcgcgagctt
     1261 cagcgccgcc acaaactcgc ttatctcttc atcagccacg acctcaaggt cgtgcgcgct
     1321 ctcgccaact cgatcatcgt gctgcgttac ggcaaggtcg tcgagcaagg cccggcgcgg
     1381 acggtgttcg ccaaaccgaa gaccgactac accaaggcgc tgctcgccgc cgccttcgag
     1441 ctcgaggcca cacgtggggg cgcggtggcg acttgataat gggaactctc aagcggtcta
     1501 cacgttgctc gggaacaaca tctctgcaac ggaggattgg accatgcgcg gagttctctt
     1561 gtggctgatc ggcattccga ttccggtgat tattctactg tggctgttcg gcgtattgca
     1621 ctagcaacca tctaaagcgt ctcggctgcc gtgatgcgcg gcagccgccg caccccaatc
     1681 acctcatagc cggcctctct gatgcgctcc ctcgcctcgg cgaagggctc gatttcgacc
     1741 gtcatgtgat gggcgttggc gtgaagcagg tcggtcgcgc tcgtcatgat gccgacgtga
     1801 ccctcccaga acacgagatc gccacggcga agctctgtgc cctcttgcca gggaagcggc
     1861 tcgccgagct ctgccgcctg catgtcggcg tcgcgcggcg ccttgtgtcc cgccgcctcg
     1921 acggcgagct ggacgaggcc tgagcagtcg agcccgatac tggtgcggcc gccccaaaga
     1981 tagggcgtgc cgagaaacgc ctcggcgatg gcgaccggat cgtccacgac ctcacccgcc
     2041 ggaagcgcgt gacgcgcaaa gacatagcct ccgtcttcga gcgcgagaaa tttgccctct
     2101 tcggctttgg cggtgaacag cgcgttcagg ctgagcaggg caagcggcgg cgacttggcg
     2161 tccggctcag
//
LOCUS       DATNAE010000253         6567 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2658854, whole genome shotgun sequence.
ACCESSION   DATNAE010000253 DATNAE010000000
VERSION     DATNAE010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6567
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2658854"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="VN518_05735"
     CDS             <1..576
                     /locus_tag="VN518_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012317708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXE04109.1"
                     /translation="VLKQLSLWDRRHSKIMTLSGGMKRRVLIAKALSHEPQVLFLDEP
                     TAGVDVELRQDMWQVVRDLRASGVTIILTTHYIEEAEDMADRIGVISKGEIILIENKA
                     ELMHKLGKKQLTLQLPQQLNSLPPELAKHNLAIEADGHELVYTYDSQGERTGITGLLQ
                     DLNAAGVRFKDLQTTQSSLEDIFVDLVRQRK"
     gene            573..1334
                     /locus_tag="VN518_05740"
     CDS             573..1334
                     /locus_tag="VN518_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXE04110.1"
                     /translation="MNLPAIRAIYKFEMARWRRTLWQSVVSPVVSTSLYFVVFGAAIG
                     TRIAHIEGVSYGAFIVPGLIMLMLLTQSVMNASFGIYFPRFAGTIYEILSAPISYLEI
                     VIGYVGAAATKSILIGLIILGTAFFFVPIRIEHPLFMLLFLVLTAVTFSLFGFVLGIW
                     ADGFERLQIVPLLIITPLTFLGGTFYSISVLPPIWQKVTLFNPVVYLVSGFRWSFFGT
                     ADVSVGVSLGMTILFLLICLAVVWWIFKTGYRLKS"
     gene            1343..2695
                     /locus_tag="VN518_05745"
     CDS             1343..2695
                     /locus_tag="VN518_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HXE04111.1"
                     /translation="MTSANTHPPKERERPALHRTIGPVQLALYGLGSMLGAGIYGLIG
                     KAAGLVGNAVWLAFVVALIAALLTALSYASLGSRYPRAAGAAYVTERAFGLPLLSFVV
                     GLALVCSGLTSVATQSRVFAANLAVLLHLESIPLWWLALGFLLVLSGIAFRGIRESMW
                     VNVLCTLVEASGLILVIAVGISYWGSVDYLEMPPRSGEESEGLILVQGAVLTFFAFIG
                     FEDTFNVAEECRDPKRTIPLGLILAMSMAAILYIAVAVTAVSVVPWPELAEAPGPITE
                     VVHRAAPLIPPVVFTGITLFAVANTALVNYVTASRLIYGMAHQGLLPLRLGEVHAERR
                     TPHLAIAALFLVAAPLALFGTIAELAAATVLLLLLVFTVVNGALFVLKRRKGEQPGYF
                     EIPRIIPALGALVCMALVVVRVATGDWQAPAIAGVLLLGAVLIYALVRSKVAPATPSP
                     "
     gene            2739..2945
                     /locus_tag="VN518_05750"
     CDS             2739..2945
                     /locus_tag="VN518_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE04112.1"
                     /translation="MDEGTIDREAMGRLAKALVFICGADHPTTVALKAAAESGSARDI
                     KAARAAFLKLKSGERKAALAMLGD"
     gene            3138..4208
                     /gene="nadA"
                     /locus_tag="VN518_05755"
     CDS             3138..4208
                     /gene="nadA"
                     /locus_tag="VN518_05755"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018329670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HXE04113.1"
                     /translation="MTTQISAVSSGKTLKPARELIPATLPDLAYTPQVARETAHLYEK
                     VSRVIPPIEWPGFAPYIKAINELKRSRNAVVLAHNYMTPEIFHCVADVVGDSLQLARE
                     AAKSDADIIVQGGVHFMAETSKLLNPDKMVLIPDSRAGCSLAASITGTDVRALREAYP
                     GVPVVTYVNTSAEVKAESDICCTSSNAVRVVESLGVPRVIMIPDEYLAKWVATQTSVD
                     IITWKGHCEVHERFTGEELRAYREADPGVKIIAHPECAPDVVAEADFTGSTSGMIKWV
                     HDHQPTKVLLVTECSMSDNVAVEAPKVNFVRPCNLCPHMKRITLPKILESLVMLKEEV
                     TIPKEIAARARVPIERMINLSH"
     gene            4234..5874
                     /locus_tag="VN518_05760"
     CDS             4234..5874
                     /locus_tag="VN518_05760"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HXE04114.1"
                     /translation="MATKDKSALDGVERRSQGGVVIVGAGLAGLFAALKLAPLPVTVL
                     SVTPFGQGGSSVWAQAGIAAALSEGDSPEAHAADTIRAGAGIVDENIALLLAREARGR
                     IEDLLRYGVPFDKDLEGHLDFAREAAHGARRILHVKGDTAGRAIMAALTVAARATPSI
                     QVLEGWGARDLVIRDGQVAAIEIARVDANPFAPSLVLDAAAVVLATGGSGQLYAITTN
                     PRESRGEGIAIAARAGATIADAEFVQFHPTAINVGRDPAPLATESLRGEGALLINGRG
                     ERFMRALHPDAELGPRDIVARGVYREVMSGRGAFLDCRKIGPEFPARFPTVFAACEAA
                     GLDPRREPIPIAPAAHYHMGGIYTDANGRTTIEGLWACGEVASTGAHGANRLASNSLL
                     EAVVFGARVAGDIAERLPSSEAIALDISAAPTDAPPSEPDSQAVQTLRRTMTGKVGVI
                     RDDASLVSALRTIAGFMRNAASDPRRDNMLTTAKLITTAALMRKESRGAHFRSDYPEA
                     DPKLARRSLLTLAEAELVAGDAVSDRERAPRWAASLSA"
     gene            5871..>6567
                     /gene="nadC"
                     /locus_tag="VN518_05765"
     CDS             5871..>6567
                     /gene="nadC"
                     /locus_tag="VN518_05765"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HXE04115.1"
                     /translation="MSAEPANLELPPFLVERAVAAALEEDLGQAGDITTDPIIPPHAT
                     AEAEIVARKAGVIAGLDLAAASFKALDPDAQFVADVADGAQVAAGAYLARAQGKARAL
                     LTAERSALNFLGHLSGIATLTAAYVAAIEGTKARIACTRKTTPGLRAFEKYAVRAGGG
                     INHRYGLYDAVLVKDNHIAAAGGLAQALAKLRACTGHLVKVEVEVDTLDQLDEALRFP
                     IDAVLLDDMDVATM"
BASE COUNT         1077 a         2206 c         2045 g         1239 t
ORIGIN      
        1 gtgttgaagc aattgtcgct gtgggaccgg cgccacagca agatcatgac tctgtccggg
       61 ggcatgaaac gccgcgtgct catcgccaag gcgctgtcgc atgagccgca ggttctcttc
      121 ctcgatgagc ccaccgccgg cgtcgacgtc gaactgcgcc aggacatgtg gcaggtggtg
      181 cgcgatttgc gcgcctcagg cgtcaccatc atcctcacca cccattacat cgaggaggcc
      241 gaggacatgg ccgaccgtat cggggtgatc tccaagggcg agatcatcct gatcgagaac
      301 aaggccgagc tcatgcacaa gctcggcaag aagcagctca ccctgcagct gccccagcag
      361 ctcaattcgc tgcctcccga gcttgccaag cataatcttg cgatcgaggc ggacggccac
      421 gagctggtct acacctacga ttcccaaggc gagcgcaccg gcatcaccgg attgttgcag
      481 gacctcaatg ccgctggcgt gcggttcaag gatctgcaga cgacgcaaag ctcgctcgag
      541 gacatcttcg tcgatctggt gaggcagcga aaatgaacct tccggcgatc cgcgcgattt
      601 acaaattcga gatggcgcgc tggcggcgca cactgtggca gagcgtggtc tcgccggtgg
      661 tttccacctc gctttatttc gtggtgttcg gcgccgccat cggcacccgc atcgcccaca
      721 tcgaaggggt gagctacggc gccttcatcg tgcctgggct gatcatgctg atgctcttga
      781 cccagagcgt catgaacgcg tccttcggca tctatttccc gcgctttgcc gggaccatct
      841 acgagatcct gtcggcgccg atctcgtatc tcgaaatcgt catcggctat gtcggcgccg
      901 ctgcgaccaa atcgatcctg atcggcctga tcattctcgg cacagccttc ttcttcgtgc
      961 cgatccgcat cgagcatccg ctgttcatgc tgctgttttt ggtgctcacc gcggtgacct
     1021 tcagcctgtt cggcttcgtg ctcggcatct gggctgacgg cttcgagcgc ttgcagatcg
     1081 tgccgctgct catcatcacc ccgctcacct ttctcggcgg cactttttat tcgatcagcg
     1141 tgttgccgcc catctggcag aaggtgacgc tgttcaaccc tgtggtctat ctcgttagcg
     1201 gcttccgctg gagcttcttc ggtacggccg atgtgagcgt aggggtgagc ctcggcatga
     1261 ccatcctgtt tcttctgatc tgcctcgccg tggtgtggtg gatcttcaag accggctacc
     1321 ggctgaagag ctaggcgatc ttttgaccag cgccaacacg catccaccca aagagcggga
     1381 gaggcctgcg ctgcaccgaa ccatcggccc ggtgcaactc gccctttacg gcctcggcag
     1441 catgctcggc gccggcatct atgggctgat cggcaaagcc gcaggcctgg tgggcaatgc
     1501 cgtctggctc gctttcgtgg tggcgctgat cgcagcgctg ctcaccgcgc tctcttatgc
     1561 ctcgctcggg tcgcgctatc cgcgcgccgc aggtgccgct tacgtcaccg agcgcgcctt
     1621 cggcctgccg ctcttgagct tcgtggtcgg gcttgcgctg gtttgctcgg gactgacctc
     1681 ggtcgccacg caatcgcgcg tgttcgccgc caacctcgcg gtcctcctgc atctggaaag
     1741 catccctctg tggtggctcg cgctcggctt cctgcttgtg ctctccggca tcgcgttccg
     1801 cggcattcgc gaatcgatgt gggtgaacgt gctctgcacc ctggtcgaag cgtccgggct
     1861 cattctcgtc attgcagtcg ggatctccta ttgggggagc gtcgattatc tggagatgcc
     1921 gccgcgctcg ggcgaagaga gcgaaggact gatcctggtg cagggcgctg tgctcacctt
     1981 cttcgccttc atcggctttg aggatacctt caatgtcgcg gaggaatgcc gcgatccgaa
     2041 gcgcaccatc ccgctggggc tgatcctcgc catgagcatg gccgcgatcc tttacatcgc
     2101 tgtcgcggtc accgcggtct cggtggtgcc gtggcccgag cttgccgaag cgcctggccc
     2161 gatcaccgag gtggtgcacc gggcggcgcc gctcattccg cccgtcgtgt tcacggggat
     2221 cactctgttc gccgtggcca ataccgcgct cgtcaattac gtgacggcgt cccgcctgat
     2281 ctacggcatg gcgcatcagg gcctgctgcc gctccgcctc ggcgaagttc atgccgagcg
     2341 tcgcacgccc cacctcgcca tcgccgcgct gttcctggtc gcggcgccgc ttgctttgtt
     2401 cggcaccatc gccgagcttg ccgccgcgac cgtgctcctg cttctgctgg tctttaccgt
     2461 ggtgaacggc gcgctgttcg tcctcaagcg gcggaagggc gagcagcccg gctatttcga
     2521 gattccccgc atcattccgg cgcttggggc gctcgtgtgt atggccctgg tggtggtgcg
     2581 ggtggcgacg ggagactggc aggcgccggc catcgccgga gtgctgctac tcggcgccgt
     2641 gctgatctac gcgctggtca gatcgaaggt ggcacccgcg acgccctcgc cgtgatagtc
     2701 tgagcttatt gacactcggc accctggagc ttccctgaat ggatgaaggc acaatcgacc
     2761 gcgaagccat gggtcgcctc gccaaggccc tggtcttcat ctgcggcgcc gaccatccga
     2821 ccaccgtggc attgaaggcc gcagccgaga gcggctcggc ccgcgacatt aaggccgccc
     2881 gcgcggcctt cctcaaattg aagtcaggcg agcgcaaggc agccttggcc atgctgggag
     2941 actgatcggg gctcgctctg gggcccctga ggcatttcgg ttacaacctt cacgttatcg
     3001 ccgacccccc ttgaagccct gcttccatcc cctatactcc aagtgagaat aggggcccgg
     3061 ccttgtgccg gatgttttaa cgccccatat atgctcagtc tgagcataat aggaaagagt
     3121 cgacgggagt aggggccatg acaacgcaga tttcggccgt atcgagtggc aaaacgctta
     3181 agccggcgcg ggaattgatc ccggcgacgc tgcccgatct cgcctacacc cctcaggtgg
     3241 cgcgcgagac cgcccatctt tacgagaagg tgtcgcgggt gatcccgccc atcgagtggc
     3301 cgggcttcgc gccttacatc aaggctatca acgagctgaa gcggtcccgc aacgccgtcg
     3361 tgcttgccca caactacatg acgccggaga tcttccattg cgttgccgat gtggtcggcg
     3421 actcgctcca gctcgcccgc gaggcggcca agagcgacgc cgacatcatc gtgcagggcg
     3481 gcgtgcattt catggccgag acctccaagc tgctcaaccc cgacaagatg gtgctgatcc
     3541 cggactccag agccggctgc tcgctcgccg cctcgatcac tggcaccgac gtgagggcgc
     3601 tgcgcgaggc ctatcccggc gtgccggtcg tcacctacgt caacacctct gccgaggtga
     3661 aggccgagtc cgacatttgc tgcacctcgt ccaatgccgt gcgcgtggtc gagtcgcttg
     3721 gcgtcccgcg cgtcatcatg atccctgacg agtatctggc gaagtgggtc gccacccaga
     3781 cctccgtcga tatcatcaca tggaagggcc attgcgaggt gcatgagcgc ttcaccggcg
     3841 aggagctccg cgcctatcgc gaggccgatc ccggcgtcaa gatcattgcc caccccgaat
     3901 gcgcccccga cgtggtcgcc gaagccgatt tcaccggctc gaccagcggc atgatcaaat
     3961 gggtgcatga ccatcagccg accaaggtgc tgctcgtgac cgaatgctcg atgagcgaca
     4021 acgtcgccgt cgaggcgcct aaggtcaatt tcgtgcgtcc gtgcaatctt tgcccgcata
     4081 tgaagcgcat cacgctgccg aagatccttg agtccctcgt catgctgaaa gaggaggtga
     4141 ccattccgaa ggagatcgct gcgcgggcgc gggtgccgat cgagcgcatg atcaatctca
     4201 gccactaacc ggtatggcga gggtacgcgt gccatggcca cgaaagacaa atctgcctta
     4261 gacggagtcg agcgtcgatc acagggcggc gtggtcattg tcggcgcagg gctcgccggc
     4321 ttgttcgcgg cgctcaagct tgcgccgctg ccggtaaccg tgctctcggt cacgcccttc
     4381 ggccaaggcg gctcaagcgt ctgggcgcaa gcaggcattg ccgccgcgct ttctgaaggt
     4441 gacagcccgg aggcgcatgc cgccgatacc atccgtgccg gtgccggcat cgtcgatgag
     4501 aacatcgcct tgttgctggc gcgcgaggcg cggggccgca tcgaggatct gctgcgctac
     4561 ggcgtgccct tcgacaagga tctcgaaggc catctcgatt tcgcccgcga ggcagcccac
     4621 ggcgcccgcc gcattcttca cgtcaagggc gatactgccg gccgcgccat catggcggcg
     4681 ctgactgtgg cggcgcgggc cacgccatcg atccaggtcc ttgagggctg gggtgcccgc
     4741 gatctcgtca ttcgcgacgg gcaggtcgcc gcgatcgaga tcgcccgcgt cgatgccaat
     4801 cccttcgccc caagcctggt gcttgatgcc gccgccgtcg ttctcgccac cggcggctca
     4861 ggccagctct acgccattac caccaacccc agagaatccc gcggcgaagg catcgccatc
     4921 gccgccagag cgggcgccac catcgccgat gcagagttcg tgcagttcca tccgaccgcg
     4981 atcaatgtcg gccgcgaccc cgcacctctc gccaccgagt cgctgcgtgg cgaaggcgcg
     5041 ctgctgatca atggccgcgg cgagcgcttc atgcgcgcgc tgcatcccga cgccgagctt
     5101 gggccgcgcg acatcgtcgc ccgcggcgtc taccgcgagg tgatgagcgg acgtggcgcc
     5161 tttctcgatt gccgcaagat cgggcccgag ttcccggcgc gctttccgac ggtgttcgcg
     5221 gcttgcgagg ctgccgggct cgacccgcgg cgcgagccga tcccgatcgc cccggccgcc
     5281 cactaccaca tgggcggcat ctataccgac gcgaacggcc gcaccacgat cgaaggactc
     5341 tgggcctgcg gcgaggtagc ctcgaccggg gcgcacggcg ccaaccggtt ggcgtcgaat
     5401 tccctgctcg aggcggtggt gttcggcgcc cgcgtggctg gcgacatcgc cgagcggctg
     5461 ccgtcgagcg aagctattgc gctcgatatc tccgcagcgc cgaccgacgc tccgccttcg
     5521 gagccggatt cacaagcggt gcaaacgctg cgccgtacca tgaccggaaa agtcggtgtc
     5581 attcgcgacg acgcctcgct ggtgtcggcg cttcgaacca tcgccggctt catgcggaac
     5641 gcggcgtcgg acccacgccg cgacaacatg ttgaccacgg ccaagctcat caccaccgcc
     5701 gcgctgatgc gcaaagagag ccgcggggcg cacttccgca gcgattatcc cgaagccgac
     5761 ccgaagcttg cccgtcgcag cctgttgacg cttgccgaag ccgagctcgt cgccggcgat
     5821 gcggtgagcg accgcgagcg ggccccgaga tgggccgcca gtctcagtgc atgagcgctg
     5881 agcccgccaa cctcgaattg ccgccctttc tcgtcgaacg cgcggtggct gcggcgctcg
     5941 aagaggatct cggccaagcc ggcgacatca cgaccgatcc catcattccg ccgcacgcca
     6001 ccgccgaggc cgagatcgtc gcgcgaaaag cgggtgtgat cgcaggcctc gatcttgccg
     6061 cggcaagctt caaggcgctc gaccctgacg cacagttcgt tgccgacgtc gccgacggcg
     6121 ctcaggtcgc ggccggagct taccttgcac gcgctcaggg gaaggcgagg gcgctgctca
     6181 cggccgagcg cagcgcgctc aattttcttg ggcatttgag cgggatcgcc acgctcaccg
     6241 cagcctatgt cgcggcgatc gaaggcacca aggcgcgcat cgcctgcacc cgcaaaacca
     6301 cgcccggctt gcgcgctttc gagaaatacg cggtacgcgc gggcggcggc ataaaccacc
     6361 gctacggcct ctatgacgcg gtgctggtga aggataacca catcgccgcg gccggaggtc
     6421 tggcgcaagc tctggccaag ctccgggcgt gcacagggca tctggtgaag gtcgaagtcg
     6481 aggtcgacac gctcgatcag ctcgatgagg cgctgcgttt tcccatcgat gcagtgctgc
     6541 tcgacgacat ggacgtggcc acaatga
//
LOCUS       DATNAE010000254         2341 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2660214, whole genome shotgun sequence.
ACCESSION   DATNAE010000254 DATNAE010000000
VERSION     DATNAE010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2660214"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..636)
                     /gene="recR"
                     /locus_tag="VN518_05770"
     CDS             complement(40..636)
                     /gene="recR"
                     /locus_tag="VN518_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011179035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HXE04116.1"
                     /translation="MASQEIETLSKALARLPGLGPRSARRAVLHLMKKRETALQPLLA
                     ALRQVADKLSTCSICGNVDTSDPCSICRDPRRDEKMLCVVEEVADLWALDRSRLFPGR
                     YHVLGGRLSALEGVGPDALSIDQLVRRVSVGGIDEVVLAMNATLEGQTTAHYLAERLE
                     EFPIRLSQLAHGLPVGGELDYLDEGTLAQALRARRPIA"
     gene            687..1589
                     /gene="fmt"
                     /locus_tag="VN518_05775"
     CDS             687..1589
                     /gene="fmt"
                     /locus_tag="VN518_05775"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007009652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HXE04117.1"
                     /translation="MRIIFMGSPEFAVPSLNALVEAGHEIACVYTQPPRPSGRGKGER
                     KTAVHESAEGLGIEVRTPKQLRNEQEQERFRALGADLGVVAAYGLILPAPILEAPKLG
                     CINVHASLLPRWRGAAPIQRAILAGDEISGVTLMRMDEGLDTGPMLVRQTLDIRGKNS
                     GQVTQEVANLGARLLVEWLERPTPAEAQPIAGTTYAAKIDKAETRLDWSKAAEQIDRQ
                     VRAFAPAPGAWFEAKGERLKLLAADAIGEGSGKAGEVLDNSLTIACGKGAIRPIKVQR
                     AGRAPMSAEELVRGFPIPKGTILP"
     gene            1586..2326
                     /gene="truA"
                     /locus_tag="VN518_05780"
     CDS             1586..2326
                     /gene="truA"
                     /locus_tag="VN518_05780"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018244535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HXE04118.1"
                     /translation="MTRWRLNVEYDGAPFMGWQRQDHGPSVQQTLEEALQRMTSENAT
                     FTAAGRTDAGVHALAMSTHVDIARELTPYRLSEGLNALVRPQPISILSVEEVADDWHA
                     RFSCIGRRYLYRILNRRAPPALDSGRVWHISVPLDLEAIREGAAHLIGRHDFTTFRSA
                     QCQSDSPVKTLEALDVSNVGEEIHITAAARSFLHHQVRSMVGCLAMVGRGQWQPDDMR
                     RALEASDRAALGFNAPPHGLYFVEAIYP"
BASE COUNT          427 a          757 c          787 g          370 t
ORIGIN      
        1 atggccatgg ggcggattta ggggcagtta tcgagccgtt caagcaatcg gccggcgggc
       61 gcgcaaggcc tgcgcaagtg ttccctcgtc gagatagtcg agctcacctc cgacgggaag
      121 tccgtgcgcc aactggctaa gacgaattgg gaattcttcc agtcgctcgg ccaagtaatg
      181 ggcggtcgtc tgcccctcga gcgtggcgtt catggcaagc accacctcat cgattccgcc
      241 gaccgacacg cgccggacga gctggtcaat gctgagggca tcggggccga cgccctcgag
      301 ggctgacaat cggcccccga ggacatggta gcgccccgga aaaagccgcg agcgatcaag
      361 cgcccaaaga tctgcgacct cctcgaccac gcagagcatc ttttcgtcgc ggcgggggtc
      421 gcggcaaatg ctgcaagggt cggacgtgtc aacattgccg cagatcgagc aggtcgacag
      481 cttgtcggcg acctgccgca aggcggcgag cagcggctgg agcgccgtct cgcgcttctt
      541 catcaggtgc agcacggcgc ggcgagccga ccgcggcccc agcccgggaa gccgggcaag
      601 agccttgctc aatgtttcga tttcctgact ggccactgtg gcaagcgagg taggcaattc
      661 ctcgcggttt cgccataggg ctgccgatgc gcatcatctt catgggatcg ccggagttcg
      721 cggtgccgag cctcaatgcg ctcgtcgaag ctgggcatga gatcgcctgc gtctatacgc
      781 agccgccacg cccctccgga cggggcaagg gtgagcgcaa gacggcggtg cacgagtccg
      841 cggaggggct tgggatcgag gttcgcactc caaagcagct ccgcaatgag caggagcagg
      901 agcgcttccg agcactgggc gcagacctgg gggtggtcgc cgcttatggg ctgatcctac
      961 cggcgccgat cctcgaggcg ccgaagctcg gctgcatcaa cgtccatgcc tcgctcctgc
     1021 cccgctggcg cggcgcagcg ccgatccagc gcgccattct ggcgggcgac gagatcagcg
     1081 gcgtcaccct gatgcggatg gacgaggggc tggacaccgg cccgatgctc gtccgccaga
     1141 cgctcgacat ccgcggtaaa aatagcgggc aagtcacgca ggaggtagcg aatcttggag
     1201 cgaggctgct ggtggaatgg ctcgaaagac cgacgcccgc ggaggcgcag ccgatcgcgg
     1261 ggacgaccta tgccgccaag atcgataagg cagagacgcg gctcgattgg tccaaggcgg
     1321 ccgagcagat cgatcgccag gtccgcgcct tcgcgcctgc acccggcgcg tggttcgaag
     1381 caaagggcga acgcctcaag ctgcttgcgg ccgatgctat tggcgagggg tcgggcaaag
     1441 ccggcgaggt gctcgacaat tcgctgacga tcgcctgcgg caagggcgct atccgcccga
     1501 tcaaggtgca gcgggccggg cgcgcgccga tgagtgcgga ggagcttgtg cgcggcttcc
     1561 caattcccaa gggcacgatt cttccgtgac tcgctggcgc ctgaatgtcg aatatgatgg
     1621 cgcccccttc atgggctggc agcggcagga tcacggcccc tcggtccagc agacgctcga
     1681 ggaagcactt cagcgaatga cgagcgagaa cgcgaccttc acggccgcgg gccgcaccga
     1741 cgcgggcgtc catgcgctgg cgatgagcac ccatgtggat atcgcgcgcg agctgacgcc
     1801 gtaccggctg agcgaagggc tgaacgcgct cgtccggccg cagcccatct cgatactgag
     1861 cgtcgaggaa gtcgccgacg actggcacgc gcggttctcc tgcatcggcc ggcggtatct
     1921 ctaccgaatc ctcaaccggc gagcgccgcc ggcgctagac tcaggtcgcg tgtggcacat
     1981 ctccgtcccg ctcgacctcg aagcgattcg cgaaggcgcc gctcacctga tcggtcgtca
     2041 tgacttcacc accttccggt cggcgcaatg ccagtcggac agcccggtga agacgctcga
     2101 agcgctcgac gtcagtaacg tcggcgagga gatacacatc actgcggccg cccgaagctt
     2161 cctccaccac caggtgcggt cgatggtcgg ctgcctcgcg atggtcggcc gaggtcagtg
     2221 gcagccggac gatatgcggc gagcgctcga agcgtctgac cgcgccgcac tcggcttcaa
     2281 cgcaccgccg cacgggctct atttcgtgga agcaatctat ccctagacga gcatttcacg
     2341 a
//
LOCUS       DATNAE010000255         2724 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_858626, whole genome shotgun sequence.
ACCESSION   DATNAE010000255 DATNAE010000000
VERSION     DATNAE010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_858626"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..238
                     /locus_tag="VN518_05785"
     CDS             56..238
                     /locus_tag="VN518_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE04119.1"
                     /translation="MQLKLAFFDPSDPPGDPLWESLAPSLWEQLDKPARYAALEILAR
                     LIARMLVTAANEASHE"
     gene            231..1442
                     /locus_tag="VN518_05790"
     CDS             231..1442
                     /locus_tag="VN518_05790"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HXE04120.1"
                     /translation="MNERAKITASHLARHAVVYLRQSSAAQVEHNRESTERQYALASK
                     ARELGWPAERIVVIDEDLGLSGGGTATRSGFARLTAEVALARVGLVLGLEVSRLARNN
                     AEWYRLIDLAGLTDTLIGDGDGLYHPAVFNDRLLLGLKGTMSEAELHILRARLNGGIR
                     NKAARGELRRGLPVGFVWGEEEGEVCFHPDEAVCTAIRQVFAQFAETGSARRVWLWFR
                     SEGLKFPLQMHQGGEIRWVEASYTAIHQVLTNPVYAGAYVYGRTRHEIVLDATGGRKK
                     RVRHLPRAEWQVLIPEHHPGFIDWQAYEANQERIARNTKPGPGRRRRKGGRRPSARAG
                     PLWSLRSPSAHPLSGSQRLARLPLPGQGSRREPRRLLPQHWRYPDRRGRHSDLRRCFG
                     AGRACRHPCRR"
     gene            1402..2436
                     /locus_tag="VN518_05795"
     CDS             1402..2436
                     /locus_tag="VN518_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE04121.1"
                     /translation="MEPAGLAATLAAAERLEAEREAALKQWRLGVERADYEAQRAERR
                     YRAVDPDNRLVARGLERDWEERLRALEAAKIELERREKERPRVISQAERDRLLALGPD
                     LAAVWNAPTTTPRDRKELLRTLIEDVTIKVDRDKAAAHLALRWKGGALDEIDLTLPRS
                     RPATVRTDEDTIALLRRLAAHYPDGVIAGILNRQERATAYGHRFTAGRVGNLRRHWGI
                     PCFEPTACASEGELLTIKKAAVVLGVAPSTIHRLLNDGIIAGEQPTPGAPWRIRLTKD
                     LKDRFNGDAGEDFLPMREAMRALGVSRQTVLQRVKRGELAAVHVMRGKQKGLRIKVIG
                     RQPDLFHPIS"
BASE COUNT          482 a          919 c          859 g          464 t
ORIGIN      
        1 acgctctccg cggccgatcg attggggtcg gcaccgccac agaaggggag ccgtcgtgca
       61 actgaagctg gcctttttcg acccgtccga tccaccgggg gatcctcttt gggagagcct
      121 cgcaccgagc ctctgggaac agctcgacaa gcccgcgcgc tacgctgcgc tcgagatcct
      181 ggcgcgcctg attgcccgta tgctggtgac ggcagctaac gaggcgagcc atgaatgagc
      241 gcgccaaaat cacggcaagc catctcgcgc gccatgccgt cgtctatctg cgtcagtcga
      301 gcgccgctca agtcgagcac aatcgcgaat cgactgagcg gcaatacgcg ctggccagca
      361 aggcccgcga actcggctgg cccgccgaac ggatcgtcgt cattgatgaa gacctgggcc
      421 tgtctggcgg cggaaccgcg acccgctccg gctttgcccg gctcactgcc gaggtggcgc
      481 tcgcccgtgt cggcctcgtg ctcggtctgg aagtctcgcg cttggcccgc aacaatgccg
      541 agtggtatcg cctcatcgat ctcgccggcc tcaccgacac gctgatcggc gatggcgacg
      601 gcctctatca cccggcggtg ttcaatgaca ggttgttgct gggcttgaag ggcaccatga
      661 gcgaagcaga gttgcacata ttgcgtgcgc gcctcaatgg cggcattcgc aacaaggcgg
      721 cgcgcggcga actccggcgc ggcctgcccg tcggctttgt ctggggcgag gaggagggcg
      781 aggtttgttt tcaccctgat gaagccgttt gcaccgccat ccgtcaggtc tttgcccaat
      841 tcgccgagac cggctcggcc cggcgcgtgt ggctgtggtt ccgctcggag gggctcaagt
      901 tcccgctgca gatgcatcaa ggcggcgaga tccgatgggt cgaggcgagc tacaccgcca
      961 tccaccaggt gctcaccaac cccgtctacg ccggcgctta cgtctatggc agaacccgcc
     1021 acgagatcgt cctcgatgcc actggcggac gcaagaagcg cgtgcgacac ctcccgcgcg
     1081 ccgaatggca ggtgctgatc ccggagcatc atccgggctt cattgactgg caggcctacg
     1141 aagcaaacca ggaacgcatc gcccgcaata caaagcctgg accaggccgg cggcgccgta
     1201 agggagggcg gcgcccttct gcaagggctg gcccgctgtg gtcattgcgg tcgccgtctg
     1261 cacacccact atcggggtcg caacgcctcg ccaggttacc actgcccggg caaggttctc
     1321 gtcgagaacc gcggcgtcta ttgcctcaac attggcggta tccagatcga cgaggccgtc
     1381 actcggacct tcgtcgctgc tttggagccg gccgggcttg ccgccaccct tgccgccgct
     1441 gagcggctgg aagccgaacg cgaagccgcg ttgaagcagt ggcggctcgg ggtcgagcga
     1501 gccgattacg aagcgcaacg cgccgagcgt cgctatcgcg ctgtcgatcc cgacaaccgg
     1561 ctggtggcgc gcggcctcga gcgcgactgg gaggagcgcc tgcgtgcact tgaggcggcc
     1621 aagatcgagc tggaacgccg cgaaaaagaa aggccacggg tgatctcgca ggccgagcgc
     1681 gatcgtctcc tcgcactcgg acccgatctc gccgccgtct ggaacgcgcc aaccaccacg
     1741 ccgcgcgata ggaaggagct gctacgcacc ctcatcgaag acgtcacgat caaggtcgat
     1801 cgcgacaagg cggccgccca tctcgcgttg cgctggaagg gcggcgcgct cgacgagatc
     1861 gatctcactc tgccgcgctc gcggccggcc accgtccgca ccgacgagga cacgatcgcg
     1921 ctgctgcgcc ggctcgcagc ccattacccc gatggtgtga tcgccggcat tctcaatcgt
     1981 caggagcggg ccacggcata tggtcatcgc ttcaccgccg gccgcgtcgg caacctgcgc
     2041 cgccactggg gtattccctg cttcgagccg acggcctgtg cgtcggaggg cgaactactg
     2101 acgatcaaga aggccgctgt cgtgcttggc gtcgccccat cgaccatcca tcgcttgctc
     2161 aatgacggca ttattgccgg cgagcagccg acacccggcg ccccttggcg tatccgcctc
     2221 accaaagacc tcaaggatcg cttcaacggt gatgccggcg aggacttcct gccgatgcgc
     2281 gaggcaatgc gcgcgctcgg cgtctcgcgc cagaccgtat tgcagcgtgt caagcgcggc
     2341 gaactcgccg ccgtccatgt catgcgcggc aaacaaaaag gcctgcgcat caaagtcata
     2401 ggccgacagc ccgacctctt ccatcctatc tcatgaacca gggggcatta tggagtacct
     2461 tccaagtatc cgcggatgtc ggcctcgaac acgtagctga ccttcttggt cacgatctgc
     2521 tcgcgtagcg cgcgcagtgc gtggtgagga ctacgccccg gccgatatcc atagctggcg
     2581 tcacagaagt cgacttaaaa gatcgcctcg agaatgcgcg ccaccgcctt ctgaagtagg
     2641 cggtctgcga ccgtgggtat tccgagcggc cgcgtaccaa tcttgcctgg ccccttgggt
     2701 atgtctaccc gccgaaccgg tgga
//
LOCUS       DATNAE010000256         2601 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_1763358, whole genome shotgun sequence.
ACCESSION   DATNAE010000256 DATNAE010000000
VERSION     DATNAE010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1763358"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /locus_tag="VN518_05800"
     CDS             complement(<1..841)
                     /locus_tag="VN518_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
                     /protein_id="HXE04122.1"
                     /translation="MASDTKAASHKDDLPEKESDTPLLDLSDAAVKKLIRSAKKRGYV
                     THEQINSVLSSEEVNSEQIEDVLAVFSEMGVNVVETEEASEEGEESREEADEEAESES
                     EGGELVEVRPKVPAKVQAKEPTDRTDDPVRLYLREMGSVELLSREGEVAIAKRIEAAR
                     EAMIAGLCENPLTFQAIIIWRDELNEGKVFLRDIIDLEATYAGPDAKAMPAPVIGSGG
                     EQMVAAGASAAPGASVLGQTPAPPAVPEGVTPIKGAPERGNVGNGGEAGEGVEPTPGE
                     GDFD"
     gene            complement(1129..2322)
                     /locus_tag="VN518_05805"
     CDS             complement(1129..2322)
                     /locus_tag="VN518_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009734973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OpgC domain-containing protein"
                     /protein_id="HXE04123.1"
                     /translation="MQAAEKAPAVQAAVVTPGRDLRLDLFRGIALWLIFLDHIPSNLV
                     AWGTLRNYGFSDATEIFVFVSGYTAAFVYGKEMRERGPLIAGAHILRRVWQIYVAHVF
                     LFAIYLAEISYVATSFENPLYAEEMNILDFLKQPDVTIVEALLLKFKPANMDILPLYI
                     VLLLLFPPILWLLQFRAWLALGASVLLYVLTWHFGWNLPSYPEGNWFFNPFAWQLLFV
                     FGAWCALGGAHRLGPVLQSRVTLAIAIAYLLFAFVIALTWYLPGMGPYVPHWLEELIY
                     PIDKQNLDVLRFAHFLALAAVTVWFIPRDAPGLKSPWLWAPIVCGQHSLEIFCLGVFL
                     SFAAHFAMIEVSGAVWMQFLLSGLGIVVMVAVAGLLTWYKRAERSSGSRPKRPPDADL
                     AGGEA"
BASE COUNT          473 a          862 c          824 g          442 t
ORIGIN      
        1 cgtcgaaatc gccttcgccg ggcgtgggct cgacgccctc gccggcctcg ccaccattgc
       61 cgacattgcc gcgctcgggc gcccctttta tcggagttac cccctcgggc acggcgggcg
      121 gcgccggcgt ctgccctagc accgaggcgc cgggagcggc cgacgccccc gccgcgacca
      181 tctgctcacc gcccgagccg atcaccggcg ccggcatcgc cttggcgtcg ggaccggcat
      241 aggtcgcctc gaggtcgatg atgtcgcgca ggaagacttt gccctcgttg agctcgtcgc
      301 gccaaataat gatggcctgg aacgtgagcg ggttctcgca caaacccgcg atcatcgctt
      361 cgcgggcggc ctcgatgcgc ttggcgatgg caacctcgcc ctcgcgcgag agcagctcca
      421 ccgagcccat ttcgcgtaga tacaggcgca ccggatcgtc ggtgcggtcg gtcggctcct
      481 tggcttggac tttggccggg accttcggcc ggacctcgac gagctcgccg ccctcgctct
      541 cgctctcggc ttcctcgtcg gcctcttcgc gcgactcctc gccctcttcg ctcgcctcct
      601 cggtctcgac cacgttcacg cccatctcgc tgaacacggc gagcacgtcc tcgatctgct
      661 cggaattgac ctcttccgag gacaacaccg agttgatctg ttcgtgggtg acgtagccgc
      721 gcttcttggc cgagcggatg agcttcttga cggccgcatc ggagaggtcg agcagcgggg
      781 tgtccgactc cttctccggc aagtcatcct tgtgagacgc cgccttggta tcgcttgcca
      841 taagcctcca cagccgacac gaacccgcag cggatcgtcc tgccgcgtag gcgataccct
      901 aaagggatgg ctgtgcctat ctaaaactta ggccgtcaat ccgatgccaa ttcacgtcgg
      961 ggcggtcgat cttgctgccg acccaccgaa atgacagcac tcggcgtcct aaacaccgag
     1021 cgtttttcgg cgcgcgcgcc ttctggacag ccacggtcgc gcgtcagccg aatatccgcg
     1081 ttctctcggt gctcgtgcgc catgatttcg cgcccgacac acgcaggatc acgcctcgcc
     1141 tcccgcgagg tcggcgtccg gcggccgctt cggccgcgac ccggaactgc gttccgcccg
     1201 cttataccac gtcagcaggc cggcaaccgc gaccatgacg acgatgccga ggccgctgag
     1261 caggaattgc atccacaccg cgcccgagac ctcgatcatg gcgaagtggg cggcgaagga
     1321 cagaaacacg ccgaggcaga agatctcgag cgaatgctgc ccgcacacga tcggcgccca
     1381 cagccaaggc gatttcaatc ccggcgcgtc gcgcggaatg aaccacaccg tcaccgccgc
     1441 cagcgcgagg aaatgcgcga agcgcagcac gtcgaggttt tgcttgtcga tggggtagat
     1501 cagctcctcg agccaatgcg gaacgtacgg gcccatgccg gggagatacc aggtgagcgc
     1561 gatgacgaat gcgaacagga ggtacgcgat cgcaatcgcc aacgtcaccc gcgactgcag
     1621 caccggcccg agccggtgcg cgcctcccag cgcgcaccag gcgccgaaca cgaagaggag
     1681 ttgccaggcg aacgggttga agaaccaatt gccttcggga tagctcggca ggttccagcc
     1741 gaagtgccag gtgagcacgt aaaggagcac cgaggcgccg agcgccagcc aggcgcgaaa
     1801 ctgcaggagc cagaggatcg gcgggaacaa cagcaggagc acgatataga gcggcaggat
     1861 gtccatgttc gccggcttga acttgagcaa cagcgcctcg acgatggtca cgtcgggctg
     1921 cttgagaaaa tcgaggatgt tcatctcctc ggcgtagagc gggttctcga agctcgtggc
     1981 gacgtaggag atttcggcga gatagatggc gaacaggaac acgtgggcga cgtagatctg
     2041 ccacacgcgg cgcaagatat gcgcgccggc gatgaggggg ccgcgctcgc gcatctcctt
     2101 gccgtagacg aaggcggcgg tgtagccgga gacgaagacg aagatctcgg tcgcgtcgct
     2161 gaagccgtaa ttgcgcagcg tgccccaggc gacgaggttc gaggggatat ggtcgaggaa
     2221 gatcagccag agcgcgatgc cgcgaaacag atcgagccgc aggtcgcgcc cgggggtaac
     2281 cacggcggct tgcaccgcgg gcgctttttc cgcggcctgc attgcaggcg agggttccgc
     2341 gccgtgcatt ccgggcgatc tttccacgct catggcatcg ctgaatctag accaggattg
     2401 gattcgctca accctgtctg ccgtcgacct agtttcattc cgttagcggg cggttggccc
     2461 gacttaggag tcatggatcg caacgcactt cagcgccgta gactcggtgc gtcccctccc
     2521 ccgcttgcag ccgaactcgg gtttacccga gttcggccac ccttgatcgg ccgaagtcgg
     2581 atacatccga cttcggctgg a
//
LOCUS       DATNAE010000257         6548 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3862508, whole genome shotgun sequence.
ACCESSION   DATNAE010000257 DATNAE010000000
VERSION     DATNAE010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6548
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3862508"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            249..2342
                     /locus_tag="VN518_05810"
     CDS             249..2342
                     /locus_tag="VN518_05810"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HXE04124.1"
                     /translation="MRIALWSLAAIIGIGVAVVFVAPLFISAEDVRNRLFAEIEEATG
                     YRLTVNGSLHISAFPTLKLVAEDVGVAQTKGAGAVDLATAKELRFGLAVAPLLSGKVQ
                     LTEVALISPILTMPEASPKTAAAEPSEKAGGGASIATALRSLSLDSLRIEDGTLLLRG
                     EGGAPAKRIDGLNLEASLPAFDAPLSVDVKARLDGQPLGVTGSISSFGPFLEGEAAQL
                     LLDVDAPAYFPQKLALMGSATYTGDVWALDGLSARAGETMLRGVVSADLSGAVPEIKA
                     SLNGDSLNVDGLLGRSAKAAAGPADGASGWSDAKIDFSGLKAVNAQVNLGVERLSYGT
                     IKAGPIGIRASVAGGKLKLELPNFQLYGGVGTGVLSVDATGKVPVQTFRFSLSNLDAY
                     PFLDDVAAFQRIEGKAAIAIDLAASGANQRAMVSALAGTASFEFNDGAIRGINVAKMV
                     RNLGAATLSGWQSGDAEKTDFASLGASFKIAQGKAQTSDLHLSGPLVRVAGAGTVDLP
                     AQTINLRVDPQVVASLEGQGGKNDLQGLGVPVAINGPWAAPSIYPDIAGILENPQAAY
                     EKLSKLGGGLVKLPSADSLGGALSSTGGIAGLVKGKAGGSIEGLIKADQGSQGVVQGL
                     GQLLGANQVAPAAAPAEPAAPQAKEPAKKKGKQKQAKAGTAAKPVGSSPAQPAAVGPA
                     KPTPKERLQHTFSAF"
     gene            2746..4296
                     /locus_tag="VN518_05815"
     CDS             2746..4296
                     /locus_tag="VN518_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HXE04125.1"
                     /translation="MAEKRHEGGISYHTPDGDYFNQRELRRHAGVFSLWALGVAAVIS
                     GDFSGWNLGFAVGGWGGMFVGTVIITIMYLGLTYSIAEMSPALPHTGGAYSFARTAFG
                     PWGGFITGVAENIEYVLTPAVIVYFIGTYLTAIFDTPAAFQPLWWIFGYIVFVGLNIR
                     GVELSFMVTIMMTLLAIGILVVFFISALPFFDFGKYALNIGADPATGAAIELPEGHGP
                     FLPFGIYGVLAAMPFAVWLFLAIEQLPLAAEESADPKRDMPKGIMLGMFTLIALGFLV
                     LLFNPSIPIERQVADASGALTTVHGAFALGTSAEPILDGFRVIFRTEAANALALAAVT
                     GLVASFHAIIFAYGRQIYSLSRAGYFPHFLSVTHGEHKTPNIALIAGGLVGLAVMVVV
                     WFAMGGEAAGSFIGGVLLNMAVFGAMFSYLLQGLTFIQLRRRFPNIERPYRSPVGVAG
                     AALTVIIALVTIGFQLADPLYRTGVIGVAIWYALAILYFAAYGRKTLVLSPEEEFAVK
                     HREGAAPV"
     gene            4366..5747
                     /locus_tag="VN518_05820"
                     /pseudo
     CDS             4366..5747
                     /locus_tag="VN518_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491347.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            5771..>6548
                     /locus_tag="VN518_05825"
     CDS             5771..>6548
                     /locus_tag="VN518_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HXE04126.1"
                     /translation="MSAPTANWSYPTAVRFGPGRIKELPDACRTAGISRPLLVTDAGL
                     AKLPITLRAIDLLKEAGFPVAMFSDVQPNPVDSNLEAGIAAFRSGGHDGVVAFGGGSG
                     LDTGKVIAFMAGQTRPLWDFEDIGDWWTRADADRIAPVVAVPTTAGTGSEVGRAGVIT
                     QEATNTKKVIFHPKMMPKVVICDPELTVGMPPAITMGTGMDALAHCLEAYCAPSYHPM
                     AEGIAVEGMRLTFANLPKAVANGNDLEARAHMMSAAAMGAT"
BASE COUNT         1146 a         2130 c         2087 g         1185 t
ORIGIN      
        1 aaaggcgcca agctatcgat catggggcga aaacgtggtt gggtgaaggt gaccaacccc
       61 gcgacgtcgg agagcggctg ggtctacgcc ggcagcaagg cgcggtgcgg aaagcagacg
      121 gaccgatcgg acgccggcgg ctcgatctgg ccgtcatggc ttggcggcgg ctagtgatct
      181 gatggttacc ggttcttagg actcctgagg gtagggttaa ccgataactc ctgcccttgg
      241 agggtagatt gcgtatcgcg ttgtggagcc tcgcggccat catcggcatc ggcgtggccg
      301 tcgtttttgt cgccccccta ttcatttccg ccgaagatgt gcggaacaga ctgttcgccg
      361 agatcgagga agcgaccggt tatcgcctca cggtcaacgg gtccttgcac atctctgcct
      421 tccccacgct caaactcgtg gccgaggatg tcggcgtggc gcagacaaag ggcgccggcg
      481 ccgtcgattt ggcgacggcg aaagagctcc gcttcggtct tgccgtggcg ccactcctca
      541 gcggcaaagt ccagctgacc gaggtcgcgc tgatcagccc aatcctcacc atgccggaag
      601 cgagccccaa gaccgcggcc gccgagccaa gtgaaaaggc tggaggcgga gcctcgatcg
      661 ccacggccct caggagcctc agcctcgaca gcctgcgcat cgaggacggc acgctgctcc
      721 tgcgcggcga gggcggcgcg ccagccaagc ggatcgacgg gctgaacctt gaagcctcgc
      781 ttcctgcctt cgacgctccc ttgtcggtcg acgtgaaggc aaggctcgac ggccagccgc
      841 tcggcgtgac cggttccatc tcaagtttcg ggccgttcct cgagggggaa gcggcgcaac
      901 tcctgctcga cgtggatgct cccgcctatt tcccgcagaa gctcgcgctg atgggcagcg
      961 cgacctatac cggcgacgtg tgggcgctcg atggcttgag cgcgagagcc ggcgaaacga
     1021 tgctgcgcgg cgtggtctct gccgacctct ccggcgccgt ccccgagatc aaggcgtcgc
     1081 tcaatggcga cagcctcaat gtcgatggct tgcttggcag aagtgccaag gcggccgcgg
     1141 gccccgctga cggcgcctca ggttggagcg atgcgaaaat cgatttctcc ggcctcaagg
     1201 ccgtcaatgc tcaggtcaat cttggcgtcg agcgcttgag ttacggcaca atcaaagccg
     1261 gccccatcgg catccgagcg tcggtggctg gaggcaagct caagctcgaa ttgcccaact
     1321 tccagctcta tggcggggtg ggcaccggcg tgttgagcgt ggatgcgacc ggaaaggtcc
     1381 cggtgcagac cttccgcttc tcgctttcca atctcgacgc ctatcccttc ctcgacgacg
     1441 tggccgcctt tcagcgtatc gaaggcaagg cggccatcgc catcgatctt gccgcgagcg
     1501 gcgcaaacca gcgcgccatg gtttcggcgc tcgccggcac cgcaagcttc gaattcaacg
     1561 acggcgccat tcgcggcatc aatgtcgcca agatggtgcg caacctcggc gctgcgacct
     1621 tgtcgggttg gcagtctggc gacgccgaaa agaccgactt cgcctcgctc ggcgcaagct
     1681 tcaaaatcgc gcaaggcaaa gcccaaacga gcgatctcca tctgagcggc ccgctcgtgc
     1741 gcgtggccgg cgccggcacc gtcgaccttc cggcgcagac gatcaatctc cgcgtcgacc
     1801 cgcaggtggt ggcgagcctc gagggccagg gcggcaagaa cgaccttcag ggcctcggcg
     1861 tgccggtcgc cattaatggg ccttgggccg cgccttcgat ctatcccgac atcgccggta
     1921 tcctcgaaaa tccgcaagct gcttacgaga agttgagcaa gctcggcgga ggcttggtga
     1981 agctaccgag cgccgacagc cttggcggcg cgttgagcag caccggcggt atcgcgggac
     2041 tggtgaaggg gaaggccggc ggctcgatcg agggtctgat caaggccgac cagggctccc
     2101 agggtgtggt gcaaggattg ggacagcttc ttggcgccaa tcaggttgct ccggcggcag
     2161 ctccagccga acctgccgcg cctcaagcca aagagcctgc caagaagaag ggcaagcaga
     2221 agcaggccaa ggcggggact gctgcaaaac cggtcggcag ctctcccgct cagcccgcgg
     2281 ccgtcggccc ggcaaagcca acacccaaag agcgcctgca gcacaccttc agcgccttct
     2341 gatcgacgct ggcttcaaga ttcacgagta agacctagcc gcccgccagg aaggtcgttg
     2401 cgaacaggat catggcaacg accaaaaggc cggtgagtca cacccagccc tgcttgagcg
     2461 ccttcgcccc gtagcactcc tctcgccatg tcggaccccc attgttggtt cctgtgaaac
     2521 caacgaagcg gggatgagag ggtttcaagc agcagcaaat atttgcagca tggggcggga
     2581 tttggcgcct cggccaagcg cgcgccaagc cggggttggt gccccggcaa tcccttataa
     2641 attaagtctt tttgccgcgg tgtcggccgg ttcggccccg aacagacata gtgcaagtgc
     2701 ggcagttaga ctaaggtgcc cgttcgcgaa agagggatcg gcgcgatggc tgagaagcgg
     2761 cacgaaggcg ggatttccta ccacaccccc gacggagact atttcaatca gcgcgagcta
     2821 cggcgtcatg cgggggtctt ttcgctgtgg gcgctcggcg tcgccgcggt catctccggc
     2881 gatttctcag gatggaatct gggctttgcc gtcggcggct ggggcggcat gttcgtcggc
     2941 accgtcatca tcaccatcat gtatctcggc ctcacctatt ccatcgccga gatgagcccg
     3001 gcgctgccgc ataccggagg cgcctattcc ttcgcccgca ccgccttcgg gccttggggc
     3061 ggcttcatca ccggcgtcgc cgagaacatc gaatatgtgc tgaccccggc ggtgatcgtg
     3121 tacttcatcg gcacctatct cacggcgatt ttcgacacgc ccgccgcgtt ccagccgctc
     3181 tggtggatct tcggctacat cgtttttgtc gggctcaata tccgcggcgt cgagttgtcg
     3241 ttcatggtca cgatcatgat gacgctcctc gccatcggca ttctcgtggt gttcttcatc
     3301 agcgcgcttc cgtttttcga tttcggcaaa tacgcgttga acatcggcgc cgacccggcg
     3361 accggagcgg caatcgagct gccggaaggc catggtccgt tcctcccctt cgggatctac
     3421 ggcgtccttg ccgccatgcc gtttgcggtc tggctgtttc tcgccataga gcagctgccg
     3481 ctcgctgcgg aagaatcggc ggaccccaaa cgcgacatgc cgaaaggcat catgctcggc
     3541 atgttcacgc tgatcgcgct cggcttcctc gttctcctat tcaatccttc aattccgatc
     3601 gaacgccaag tcgccgacgc gagcggcgcg ctcactacgg tgcacggcgc cttcgccctc
     3661 ggcacctcgg ccgagccgat cctcgacggc ttccgggtga tcttccgcac ggaagccgcg
     3721 aacgcgctcg ccttggctgc cgtcaccggg ctggtggcga gctttcacgc catcatcttc
     3781 gcctacggac gtcagatcta ctccctgtcg cgggccggct acttcccgca tttcctgtcg
     3841 gtgacccacg gcgagcacaa gacgccgaac atcgccttga tcgccggcgg cctggtcggg
     3901 ctcgcggtga tggtggtcgt gtggttcgcc atgggcggcg aggcggctgg atcgttcatc
     3961 ggcggcgtgc tgctcaacat ggcggtgttc ggggccatgt tctcctatct gttacagggg
     4021 ctcaccttca tccagcttcg ccgccgcttt ccaaatatcg agcggccgta tcgcagcccg
     4081 gtcggcgtgg ccggggcggc gctcaccgtg atcatcgcgc tcgtgaccat tggcttccag
     4141 ctcgccgatc ccctatatcg caccggtgtg atcggcgtgg cgatctggta cgcgcttgcc
     4201 atcctctatt tcgcggccta tggacgaaag acgctcgtct tgtcgccgga ggaggagttc
     4261 gcggtgaagc accgcgaggg cgcggccccg gtgtagccga cgcagaaacc ggattgcaac
     4321 gaagccggct cgcgttcgcg ggtcggcgct tcttgtcgag gtaccatgtc agaaagcgtg
     4381 aaaatcatct ccccgatcga cggctcggtc tacgccgagc ggccgatcgc aagcgacgcc
     4441 gagatcggcg ccgctctgtc agcagcgcgc gcggcactcc ccgagtggcg gaaggttccg
     4501 gtggccgagc gcgggcgtta catgcgggcc ttcctcgatg cgctgctcgc catgaacgac
     4561 gacattgccg tggaactcgc ctggcagatg ggaaggccgg tccgctatgg cggcgagaag
     4621 ggcggcatcg aggagcgcat ccgcgccatg gtggtcctgg ctgaagaggc gctcaagcct
     4681 tacaccccgc cggcgaagga tggcttccgc cgctatatcg cgcgcgagcc agtcggcatc
     4741 gtcatggtca tcgcgccgtg gaactaccct tatctcacgg cgatcaacac catcgttccc
     4801 ggacttcttg ccggcaacgc catcatcctc aagcacgcgg cgcagacgct gctagtcggc
     4861 gagcgcatcg ccgaggcgtt ccggcgtgcc ggcctgccta aaggtctgtt cgccaatctc
     4921 gtcctgtcgc atgagcagac cgagagactg atcggttcgg gcaagatcga ccacatcaac
     4981 ttcaccggct cggtggccgg gggccgcgct atcgagcgcg ctaccgccgg caccttcgcc
     5041 tcgctcgggc tcgagctcgg gggcaaggac ccggcttacg tgcgcgccga cgccaagctc
     5101 gatcatgcca tcgagaatct ggtcgacggc gccttctaca attccggcca atgctgctgc
     5161 ggggtggagc gcatctatgt cgatgccacc gtgtttgacg atttcgtcga aggctacgcc
     5221 gatctcaccc ggaaatatgt ggtcggcaat ccgcttgatg cctcgaccac gcttgggcca
     5281 atggcgcgcg cctccttcgc cgacacggtg cgcggccaga tcgccgaggc gcggcgcaag
     5341 ggcgcgacgg ccctgatcaa catgaaggtc gatggcgacc gggctggctc gccctatctc
     5401 gcgccggagg tgctcaccaa cgtcaaccac cagatggagg tgatgcgcga ggaaagcttc
     5461 gggcccgtgg tcggcatcat gaaggtgcgc gacgacgagg aggcgatcac gctgatgaac
     5521 gacagcccct atggactgac ggcctcgatc tggacgcgcg ataccgaccg cgccgccgag
     5581 atcggaagca gggtcgagac cggcacggtc tatatgaacc gctgcgacta tctcgatccg
     5641 caactcacct ggaccgggtc aaggacaccg gccgcggcgc ggccttgtcg aaatacggtt
     5701 tcgacgcgct aacccagccg aagagctttc acctccgcga agtctgacca tctctcattc
     5761 gggacaacac atgagcgcac caaccgcaaa ttggagctac ccgaccgccg tccgctttgg
     5821 cccaggccgc atcaaggagc tccccgacgc ctgccgcacc gccggaatct cccggccgct
     5881 gctggtcacc gatgcgggcc tggccaagct tcccattacg ctgagggcga tcgacctgtt
     5941 gaaggaagct ggattcccgg tggcgatgtt ctcagatgtc cagccgaacc cggtggactc
     6001 aaatcttgag gctggcatcg ccgccttccg ctcaggcggc cacgatgggg tggttgcctt
     6061 cggcgggggc tcgggcctcg acaccggcaa ggtcatcgcc ttcatggccg ggcagacaag
     6121 gccgctttgg gatttcgagg atatcggcga ttggtggaca cgggccgatg cggacagaat
     6181 cgccccggtc gtcgccgtgc cgaccacggc ggggaccggc tctgaggtcg gccgcgccgg
     6241 cgtcatcacg caggaggcga ccaacaccaa gaaggtgatc tttcatccca agatgatgcc
     6301 caaggtggtg atctgcgatc cggagctcac ggtcggcatg ccgccggcga tcaccatggg
     6361 caccggcatg gatgcgctcg cccattgtct cgaagcctat tgcgccccga gctaccatcc
     6421 gatggcggag ggtattgccg tggagggcat gcggctcacc ttcgccaacc tgccgaaggc
     6481 ggtggcgaac gggaacgatc tcgaagcccg cgcgcacatg atgagcgcgg cggcaatggg
     6541 ggcgaccg
//
LOCUS       DATNAE010000258         2190 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_4471151, whole genome shotgun sequence.
ACCESSION   DATNAE010000258 DATNAE010000000
VERSION     DATNAE010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4471151"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1546
                     /locus_tag="VN518_05830"
     CDS             <1..1546
                     /locus_tag="VN518_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE04127.1"
                     /translation="TRSTAEAFGKHTAQLNQVLANNSAMMQQTANQVGAQSKEAVGVL
                     TSQTQTLREVSRGLLDQIHGLTQRFENQGQAILTAAKALDSSNTKIDSILEGRHQAII
                     ALLHTVNTKAQDLDNMMRSYAGMMENTLSQAEHRAKQVGTALARDTAGQAQAALTQIE
                     RLREEAQSHTNRAVADLKGSFEQIITQIGRQLEQMRGQFDTTSRGMHEVAQKTATDLD
                     GLRQEMQKRMETLPEQTAQTTAAIRKALSDQVKEIEAITPALTRAATQAAVQAAPAPL
                     PAAPMPTGGGDPFHRGGQGQAPSGGFDLPPVGRGFAPREDVRPATGQYDIPPMPKFDV
                     RGRQVASGEGGDLDQVTNSLAQQLGGASYSPQGGTRGGNFGMSAMPEQGYGGGQAFGN
                     RGVQQASSPLGGGNQLRLDELARAIDQRTASDVWYRFRSGDRGALGRHIYSLDGQATF
                     DEIARRYDRDTDFRGTVDRYIGDFERLLGEAEASDPDGRMLQNYLVSETGRVYLLLAH
                     ASGRLR"
     gene            complement(1717..>2190)
                     /locus_tag="VN518_05835"
     CDS             complement(1717..>2190)
                     /locus_tag="VN518_05835"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HXE04128.1"
                     /translation="FAAAHKAASWQLVVYEPTAIHALETDRLMVRPSADQVSYYKGVA
                     WSDRLPRLVQARIIETFQNSGAVRTVSATNGQYALVTDLRAFQIDVSSGKAIAEIEIF
                     AKLVNVNSGKALATKSFSARVPAATDAPTDAIAALNQGFTEVLQDTTTWVASRRV"
BASE COUNT          418 a          729 c          718 g          325 t
ORIGIN      
        1 tacgcgctcg accgccgagg ccttcggcaa gcacactgca cagctcaatc aggtgctcgc
       61 caacaattca gcgatgatgc agcagaccgc aaaccaagtc ggcgcgcagt cgaaggaagc
      121 cgtcggcgtg ctaaccagcc agacgcagac gctgcgcgaa gtgtcgcgcg gactgctcga
      181 ccagatccac ggtctcaccc agcgcttcga gaatcagggc caggcgatcc tcaccgccgc
      241 caaggcgctc gactcgtcga acaccaagat cgactcgatc ctcgagggcc ggcatcaggc
      301 aatcatcgcc ctcctgcaca cagtcaacac caaggcgcaa gacctcgaca acatgatgcg
      361 gtcctacgcg ggaatgatgg agaacaccct ctcgcaggca gagcataggg cgaagcaggt
      421 gggcaccgcg ctggcccgcg acaccgcagg tcaggcgcag gccgcgctca cccaaatcga
      481 gcggctccgc gaggaggctc agtctcacac caaccgcgcc gttgccgatc tcaagggcag
      541 cttcgagcag atcatcactc agatcggccg ccagctcgag cagatgcgcg gtcagttcga
      601 caccacctcg cgcggcatgc acgaggtggc gcagaagaca gcgaccgatc tcgatgggct
      661 ccgccaggag atgcagaagc gcatggagac gctgcccgag cagaccgctc agacgactgc
      721 cgcgatccgc aaggcccttt ccgatcaggt gaaggaaatc gaggcgatca caccggcgct
      781 gacgcgggcg gcgacgcaag ccgccgtgca agcggcgccg gcgccgctac ctgcagcgcc
      841 tatgccgacg ggcggcggcg atcccttcca tcgcggcggc caagggcaag cgccctcagg
      901 cggcttcgac ctgccgccgg taggccgcgg cttcgcgccc cgcgaggatg tgaggccggc
      961 aacgggtcaa tacgacatac cgccgatgcc gaaattcgac gtgaggggcc gtcaggtcgc
     1021 ttcgggcgag ggtggagacc tggaccaggt caccaacagc ctcgcgcaac agctcggcgg
     1081 cgcgtcctat agcccacaag gcggcacccg cggcggcaat ttcggaatga gcgccatgcc
     1141 cgagcagggc tatggcggag gccaggcctt cggcaaccgc ggcgttcagc aagcctcctc
     1201 ccctctcggc ggcggcaatc agctcaggct cgatgagctc gcaagagcca tcgaccagcg
     1261 taccgcgtcc gatgtctggt atcgcttccg gtcgggagac cgcggcgcgc tcggacgcca
     1321 tatctacagc ctcgacggtc aggccacgtt cgacgagatc gcccgccgct acgatcgcga
     1381 tacagatttc cgcggcacgg tcgatcgcta tatcggcgat ttcgagcggc tgctcggaga
     1441 ggccgaagcg agcgatccgg acgggcgcat gctgcagaac tatctcgtgt cggagacggg
     1501 ccgcgtctat ctcttgctcg cccatgcgag cggccggctc cgttagacgc tagcctctca
     1561 attaggctcc cccagccttc gccccccgcc ctcccctgaa agggaggcgg ggggcgagtg
     1621 attttggggg cgcccgtcgc gaaggatgag ggcaagacta cgcggcatgc ttcgagacgc
     1681 acctgcggcg cccctcagca tgaggcttgt taaaagctaa acgcggcggg aggcgaccca
     1741 ggtggtcgtg tcttgcagga cctcggtaaa gccctggttg agagcagcga tggcgtcggt
     1801 tggggcatct gtcgctgccg gcacgcgcgc gctgaagctc ttggtcgcga gcgccttgcc
     1861 tgagttcaca ttgaccagct tggcaaagat ctcgatctcg gcgatggcct tgccgctcga
     1921 gacgtcgatc tggaaggcac gcagatcggt cacgagggcg tattggccat tggtcgcgct
     1981 gactgtccgc accgcgcccg agttctggaa ggtctcgatg atgcgcgcct ggacgagacg
     2041 cggcagccgg tcgctccagg cgacgccctt gtagtaagag acctggtcgg cgctcgggcg
     2101 caccatgaga cggtcggtct cgagcgcatg gatggccgtc ggctcgtaca cgacgagctg
     2161 ccaggacgca gccttgtgcg cggcggcgaa
//
LOCUS       DATNAE010000259         2448 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3863777, whole genome shotgun sequence.
ACCESSION   DATNAE010000259 DATNAE010000000
VERSION     DATNAE010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3863777"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VN518_05840"
     CDS             <1..190
                     /locus_tag="VN518_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE04129.1"
                     /translation="TDQVADKPSDPNLGTAETAPADVPAYADRAEPWDNPPAAAAQPA
                     APAEALPAPSMDGSRYGE"
     gene            complement(194..1024)
                     /locus_tag="VN518_05845"
     CDS             complement(194..1024)
                     /locus_tag="VN518_05845"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HXE04130.1"
                     /translation="MFLNVFTATEPEAQAPKTNGAYETIIVSAKGKVGVIMLNRPEAL
                     NALNAELIGEVNRALDRFEADPAIGCIVITGNEKAFAAGVDIKEMHKKTFVEAYGSDF
                     LAPFDRISSFRKPILAAVAGYALGGGCELAMACDIILAADTAVFGQPEITLGIMPGNG
                     GTQRLTRAVGKAKAMELCLTGRRMNAEEAERSNLVSRVVPAAELMDEAMKIADKIANL
                     SQHAVMMIKEAINRGFATTLAEGSRFERGQFYSLFATEDQKEGMAAFIEKRKPVFTNK
                     "
     gene            complement(1040..1924)
                     /gene="mutM"
                     /locus_tag="VN518_05850"
     CDS             complement(1040..1924)
                     /gene="mutM"
                     /locus_tag="VN518_05850"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HXE04131.1"
                     /translation="MPELPEVETVRKGLEPILVGNAFARVEQRRKDLRFPLPKHFAER
                     LKGRTVEALDRRAKYLLARLDDGEVLVMHLGMTGRFSIDHANGEAEKPGRFVHSQADP
                     PKHEHIVFHLGDGATIRYSDARRFGLMDLVRADGLSSYALFKGLGVEPLSSSFTPEWL
                     ATKLKGKATSIKAALIDQRLIAGLGNIYACEALFRARISPKRLAGTLATKTGKATQKT
                     KALVDSVKAVLEDAIKSGGSSLRDYKRADGSLGEFQHRFEVYGREGKPCARKGCGGTV
                     RRIVQGGRSTFYCPTCQH"
     gene            2005..>2448
                     /locus_tag="VN518_05855"
     CDS             2005..>2448
                     /locus_tag="VN518_05855"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXE04132.1"
                     /translation="MSEDLENARPEEPKTSFGFATVAEGAKQGLVNAVFARVARNYDL
                     MNDLMSGGLHRLWKSEFVNLLAPPKSAVAYALLDVAGGTGDIARKVLDAAGSGTAAVI
                     CDISAEMMQVGRARAEGLGDRLAFVQGNAESLPFAARSFDAYTIAF"
BASE COUNT          426 a          768 c          785 g          469 t
ORIGIN      
        1 gacggaccag gtcgccgaca agcccagcga tccaaatctc ggaacggcgg aaaccgcgcc
       61 cgccgatgtg ccggcctatg ccgatcgcgc cgagccctgg gacaatccgc cggccgccgc
      121 cgcacagcct gctgcgcccg ccgaggcctt accggcgcca tcaatggacg gatcgcgcta
      181 cggcgaatag cacttacttg ttggtgaaaa cgggcttgcg cttctcgatg aaggcggcca
      241 tgccttcttt ctggtcctcg gtcgcgaata gcgaataaaa ctgcccgcgt tcgaaacgcg
      301 agccttcggc aagcgtcgtg gcgaagccgc gattgatcgc ctctttgatc atcatcaccg
      361 cgtgctgcga gagattggcg atcttgtcgg cgatcttcat cgcttcatcc atcagctctg
      421 cggccggcac cacgcggctc accagattgg agcgctccgc ctcctcggca ttcatgcgcc
      481 gccccgtgag gcagagctcc atggcctttg ccttgccgac cgcgcgggtg agccgctgcg
      541 tgccgccatt gccgggcatg atgccgaggg taatctccgg ttgaccgaac acggccgtgt
      601 cggcggcgag gatgatgtcg caggccatgg cgagctcgca gccgccaccg agcgcatagc
      661 cggcgacggc agcgaggatc ggcttgcgga agctcgagat gcgatcgaag ggcgcgagga
      721 agtcgctgcc ataggcctcg acgaaagtct tcttgtgcat ctccttgatg tccacgccgg
      781 cggcgaaggc cttctcgttg ccggtgatga cgatgcagcc gatcgcgggg tcagcctcga
      841 aacggtcgag cgcgcggttc acctcgccga tcaactcggc gttgagcgcg ttcagggctt
      901 ccgggcggtt cagcatgatg acgcccacct tgcccttggc tgagacgata atcgtttcat
      961 aggcgccgtt cgttttcggc gcctgagctt ctggttcggt agcggtgaag acgttcaaga
     1021 acatggtcgt gccagtcccc taatgttgac aggttggaca gtagaaggtc gaacgtcctc
     1081 cctggacgat gcggcgtacg gttcctccgc agccttttct tgcgcagggt ttgccctcgc
     1141 gaccgtagac ctcgaatctg tgctggaact ctccgagcga gccatcggcg cgcttgtaat
     1201 cgcgcagcga tgagccgccg gatttgatgg cgtcctcgag caccgccttg acgctatcga
     1261 cgagcgcttt cgtcttctgc gtcgcctttc cggtcttggt ggcaagcgtg ccggcgagcc
     1321 ttttcggtga gatgcgcgct ctgaacaagg cctcgcaggc atagatgttg ccgagccccg
     1381 cgatcagcct ctgatcgatg agcgccgcct tgatcgaggt cgccttgccc ttgagcttcg
     1441 tggcgagcca ttccggcgtg aaagaggagg agaggggctc gacgccgagg cccttgaaca
     1501 gggcgtagct cgaaagtcca tcggcgcgga cgaggtccat cagcccgaag cggcgcgcat
     1561 ccgaatagcg gatcgttgca ccgtcgccga gatggaagac gatatgctcg tgcttgggcg
     1621 gatctgcctg gctatgcacg aagcggccgg gcttctcagc ctcgccattg gcgtggtcga
     1681 tcgagaagcg gccggtcatg ccgagatgca tgacgagaac ctcgccatcg tcgagcctcg
     1741 ccaagagata tttcgcgcgc cggtcgagag cctcgaccgt ccgccccttg agccgctcgg
     1801 cgaagtgctt cggcagagga aagcgcaggt ctttccggcg ctgctcgacg cgcgcgaacg
     1861 cattgccgac caagatcggc tcaaggccct tccgcacggt ttcgacctct ggaagctcag
     1921 gcatggtgtt tatcgcagcg caaaagaaac tcgttgcgct gcaacaatag cctgatcttg
     1981 ggccttacgc tattgtccgc atcgatgtcc gaagacctcg agaatgcccg ccctgaggag
     2041 ccgaagacga gctttggctt cgccacggtc gccgagggcg ccaagcaggg cctggtgaac
     2101 gcggtttttg cccgggttgc ccgcaattac gacctgatga acgacctcat gtcgggaggg
     2161 ctccaccggc tgtggaaaag cgagttcgtg aacttgctgg ccccgccaaa aagcgccgtc
     2221 gcttacgcgc tgctcgacgt tgccggcggc accggcgata tcgcgcggaa agtcctcgat
     2281 gcggcgggct ccggcacagc cgcggtgatc tgcgacatca gcgccgagat gatgcaggtt
     2341 gggcgagcga gggcagaggg attgggcgat cgcctcgcct tcgtgcaggg caatgcggaa
     2401 tccttgccgt tcgccgcgcg gagtttcgac gcctacacca tcgccttc
//
LOCUS       DATNAE010000260         2462 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_863866, whole genome shotgun sequence.
ACCESSION   DATNAE010000260 DATNAE010000000
VERSION     DATNAE010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_863866"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1071
                     /locus_tag="VN518_05860"
     CDS             <1..1071
                     /locus_tag="VN518_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HXE04133.1"
                     /translation="MRDWRADKEINMDPALIDLIWTLHKQLGSNVPVNLICGLRTAQT
                     NESLRKKGGGQAKRSQHILGKAADITFPDVPVKVLRNSALIQEHGGVGYYPTSGIPFV
                     HVDTGNVRMWPRIPRLELAALFPNGRTKYLPTDGKPITPADYKLALANGMVNKTMFAA
                     ATPAAKAKWQTAPDTDAFDEAESDAEAETDAPQPVLASAAPPVIAEDPHEPGTPPAKL
                     FTYASAGGMSLPFFGHKPPKPTAAPLPTYEDAEVVSGPEADDDHPDALAYVPFEIAGL
                     MTDASVTYSRTVAPLSPPDQQNLDYLFDDMDQPTSLTLRKSSGYVSLAASQRFSGQAV
                     RSIYAEAQPQTAAPTRLAQSTR"
     gene            complement(1109..1390)
                     /locus_tag="VN518_05865"
     CDS             complement(1109..1390)
                     /locus_tag="VN518_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2312 domain-containing protein"
                     /protein_id="HXE04134.1"
                     /translation="MRGIAKVSLQTSAKDQLRTIVARIERLEEEKAALSTDLKEVYAE
                     AKGNGFDVKALRTVIRLRKQDESKRREEEAVLATYLHALGMAAAEAEAA"
     gene            complement(1510..>2462)
                     /locus_tag="VN518_05870"
     CDS             complement(1510..>2462)
                     /locus_tag="VN518_05870"
                     /inference="COORDINATES: protein motif:HMM:NF016873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1244 domain-containing protein"
                     /protein_id="HXE04135.1"
                     /translation="GAAQVAEHLARALGAPAVLSRFSRLLIDPNRGLDDPTLIMQISD
                     GLVVPGNVDLAAAEIEARIAGYYRPYHAAIAQAVDAAIAAGRPPVLISVHSFTQAWKG
                     VPRPWAVGVLWDKDPRLALALLEGLRVIPDIEVGDNVPYSGQLKGDTLYRHGTMRGLA
                     HALVEVRQDLILGPEDQKQWAMRLAKVLGTVLSQPGSALHAIELHGSHTDIAPHDAPG
                     KKGAARMDDKTEIELEAAAFRRLVAHLRERADVQNIDLMELAGFCRNCLADWYREAAA
                     AKGLPLSKEEAREIVYGMAYDAWKAKYQTAAPTKPRKRIG"
BASE COUNT          426 a          864 c          745 g          427 t
ORIGIN      
        1 atgcgcgatt ggcgggccga caaagaaatc aatatggacc ccgctttgat cgacttgatc
       61 tggaccctgc acaagcagct cggctcgaac gtcccggtca atctcatttg cggccttcgg
      121 accgcgcaga ccaacgaatc actgcggaag aaaggcggcg gtcaggcaaa gcgcagccag
      181 catatccttg gcaaagccgc cgacatcacc tttcccgacg ttcccgtaaa agtactgcgc
      241 aattccgcgc tgatccagga gcatggcggc gtcggctatt acccgacctc gggcatcccc
      301 ttcgtccacg tcgataccgg caatgtccgc atgtggccgc gcatcccgcg gctcgagctt
      361 gcggcgctct tccccaacgg cagaaccaaa tatctgccga ccgacggcaa gccgatcacc
      421 ccggccgatt acaagctcgc cctcgccaac ggcatggtca acaagaccat gtttgctgcg
      481 gcgacgccgg ccgccaaggc caagtggcag accgcgcccg ataccgacgc gttcgacgag
      541 gccgagagcg atgccgaggc cgaaaccgac gcgccgcagc cggtgctcgc ttcggctgcc
      601 ccgcctgtga tcgccgagga tccccacgag ccgggcacgc ctccagccaa gctcttcacc
      661 tatgccagcg ccggcggcat gtcgctgccg ttctttgggc acaagccccc caagccgacc
      721 gctgcgcccc tgcccaccta tgaggacgcc gaggtcgtga gcgggccgga ggccgacgat
      781 gaccatcccg acgcgctggc ctacgttccc ttcgagatcg ccgggctgat gaccgatgcg
      841 tcggtcacct atagccgcac cgtggcgccg ctcagcccgc ccgatcagca gaacctggac
      901 tatctgttcg acgacatgga ccagccgacc tcgctcaccc tgcgcaagag ctcgggctat
      961 gtcagcctgg cagcgtccca gcgcttctcc gggcaagcgg tgagaagcat ctatgcggag
     1021 gcgcagccgc agaccgccgc gccgacgcgc ctcgcccaga gcacccgcta gccactctca
     1081 tttcactgag acgctgtcat ctgaaggcct aggccgcctc ggcctcggcg gcggccatgc
     1141 caagcgcgtg gagataggtg gccagcaccg cctcctcctc ccgccgcttg ctctcatcct
     1201 gcttgcgtag gcggatcacc gtccgcaatg ccttcacgtc aaagccgtta cccttggcct
     1261 ccgcatagac ttccttcagg tcggtgctga gcgccgcctt ttcttcctcg agccgctcga
     1321 tgcgggcgac gatggtacga agctggtctt tggctgatgt ctgaagcgac actttggcga
     1381 tcccccgcat ggcttgtgat tcgatttcga cccaagctta ggtcgcttgg atttgacccg
     1441 gcaaggcgcg cgccgactta tcccgcttat ccaaagcgcg cgccggttga gggcggaaaa
     1501 aatgggctgt cagcctatgc gcttgcgcgg cttggtgggc gccgccgtct gatacttggc
     1561 cttccacgcg tcataggcca tgccgtagac gatctccctc gcttcttcct tgctaagcgg
     1621 caagcctttc gccgcggcgg cctcgcgata ccagtcggcg aggcagttgc ggcagaagcc
     1681 ggcaagctcc atgagatcga tattctgcac gtcggcgcgc tcgcgcagat gcgcgacgag
     1741 gcgccggaag gcggcggctt cgagctcgat ctcggttttg tcgtccattc tcgctgctcc
     1801 ttttttgccc ggcgcgtcat gcggcgcaat gtcggtatgc gagccgtgca gctcgatcgc
     1861 atgcagcgcg ctgcccggct ggctcagcac ggtgccgagc accttggcga gccgcatcgc
     1921 ccattgcttc tggtcctcgg gtcccaggat cagatcctga cggacctcga ccagagcatg
     1981 cgccaagccg cgcatagtcc cgtggcgata cagcgtgtcg cccttgagtt ggcccgaata
     2041 aggaacgttg tcgccaacct cgatgtccgg gatgacgcgc aagccttcga gcaaggcgag
     2101 cgccaatcgc gggtccttgt cccacaacac cccgaccgcc cacgggcgcg gcacgccctt
     2161 ccaggcctgg gtgaagctat gcaccgagat aagcaccggt ggcctgccgg cggcgatcgc
     2221 cgcatcgacg gcctgcgcga tcgccgcgtg gtagggccgg taatagcccg cgatgcgggc
     2281 ttcgatctcc gccgccgcca gatccacgtt gcctggcacg accagcccgt cggagatctg
     2341 catgatcagg gtcgggtcgt cgaggccacg attggggtca atcagcaggc gcgagaagcg
     2401 cgagagaacg gccggcgcgc cgagcgcccg agccagatgc tcggccactt gcgcggcgcc
     2461 ca
//
LOCUS       DATNAE010000261         2985 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_1768168, whole genome shotgun sequence.
ACCESSION   DATNAE010000261 DATNAE010000000
VERSION     DATNAE010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1768168"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1527)
                     /locus_tag="VN518_05875"
     CDS             complement(<1..1527)
                     /locus_tag="VN518_05875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXE04136.1"
                     /translation="MAKLAGQMLTTRGVAAFWLLWSLAFVLMLWAVSPGRTLQDALAA
                     ELLQGHLAGGYQLRNPPLYEWLLWGVQQAVGPGPLSYLILRYALIATTGILFTLAVQR
                     TVGSARVAAAFSLSLVLFFWFGWEIHQSVSHTLALLAAVLALFIVSLAYAERPTAMRA
                     FLLGLIMGLGLMAKWSFLLVLLSLGVALAVTPETRGIFRERRTLLVLAGAALPILPFA
                     AWLATIDPDLVGRRTIPSGRALSLDQALEGVAAFVTGIPLVFLPWIAFVLFFAWRFPK
                     APPLSAMLSHAVAIRLASVTAIVTLALMTALLLSAFATGGALFGISRFAIHYLFPFSL
                     FAALALAGLVAQRVEDRRFGQRLAIVSLAVALVIFFVKLASFYVVPGGSEATNLFPYA
                     RLADELTKRGLGTAQFVTLSPRDAGNLAIYLPEARALSLSARIEPPPPDPVPDRPCVL
                     LWGGEFSVPPAAPPEPNPSPGKLLKPLGVGQSTKGAEDVVVDWPKPLIGAQRRSVWHL
                     L"
     gene            1605..>2985
                     /gene="rimO"
                     /locus_tag="VN518_05880"
     CDS             1605..>2985
                     /gene="rimO"
                     /locus_tag="VN518_05880"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HXE04137.1"
                     /translation="MPKVPAQKTPPRSGPPRIGFVSLGCPKALVDSERIITKLRAEGY
                     ELCSSYEGADAVVVNTCGFLNSAKEESLAAIGEALAENGKVIVTGCLGVESDRIRAVH
                     PGVLAITGPHQYEAVVEEVHKVVPRPHDPFLDLLPPQGIRLTPRHYAYLKISEGCNNR
                     CSFCIIPHLRGRLASRPIGDVMREAERLVDAGVKELLVISQDTSAYGLDLGYARSKWR
                     GEEREARFLSLAEALGSLGAWVRLHYVYPYPHVDQVLPLMADEKVLPYLDIPFQHASP
                     TVLKAMRRPAHQEKTLERISSWRETCPDLAIRSTFIVGFPGETEEDFDFLLEWLSEAR
                     LARVGCFKYEDVDGAAANALPGQVPQEVKEERYARLMQHQQAISAEGLAERVGKTIEI
                     IVDEVDSNGAIARSTWDAPQIDGNVYLNGETGLSPGDRARVTVEGSDEYDLWGTLAEN
                     SAAQTPEPAR"
BASE COUNT          601 a          964 c          982 g          438 t
ORIGIN      
        1 caagagatgc cacaccgaac gccgctgcgc cccaatcagg ggctttggcc aatccaccac
       61 gacatcttcc gcgcccttcg tgctctggcc gacaccgagc ggcttcaaca gcttgcccgg
      121 tgatggattc ggctccggcg gcgcagccgg cggcacggaa aattcgccgc cccacaacag
      181 cacgcaggga cgatcgggca cgggatcagg cggcggcggt tcgatcctcg cgctcaacga
      241 cagcgcccgc gcctcaggca aatagatggc gaggttgcct gcgtcgcgtg gcgacagcgt
      301 cacgaattgc gctgtgccga gccctcgctt tgtgagctcg tctgccaagc gcgcataagg
      361 gaagaggttg gtcgcttccg agcccccggg caccacatag aagctcgcga gcttgacgaa
      421 aaaaatcacg agggccacgg cgagcgaaac gatcgcgagc ctctgcccga aacgccgatc
      481 ctccacgcgt tgcgcgacga ggccggcaag cgcgagcgcc gcgaacagag agaacgggaa
      541 gagatagtgg atggcgaagc ggctgatgcc gaagagtgcg ccgcccgttg caaacgcgga
      601 gaggagaagc gctgtcatca gcgccagcgt cacgatcgcc gtgacgcttg cgagcctgat
      661 cgccaccgcg tgagacagca tggccgataa cgggggcgcc ttggggaacc gccaggcaaa
      721 gaacagcacg aaggcaatcc acgggagaaa cacgagcggg atgccggtga cgaaggcggc
      781 gacgccctcg agcgcctgat cgagcgacag cgcgcgccca gagggtatcg tgcgccggcc
      841 gacaaggtcc gggtcgatcg tggcgagcca tgcagcgaag ggaaggatcg gcagcgcggc
      901 gcctgccagc acgagcagcg tgcggcgctc gcgaaagatc ccccgcgtct caggcgtcac
      961 ggcgagcgcc acgccgaggc tcagcagcac gagcaggaag ctccattttg ccatgagccc
     1021 gaggcccatg atcagtccga gaaggaatgc gcgcatggcc gttggccgct cggcataggc
     1081 gaggctcacg ataaacagcg caagcactgc ggcgagcagc gcgagcgtgt gggagacgct
     1141 ttgatggatc tcccagccga accagaagaa cagcacgagt gagagggaaa aggcggcggc
     1201 aacgcgggcg cttccgaccg tgcgctgaac cgccaacgtg aacaggatgc cggtggttgc
     1261 gatcagggcg tagcgcagga tgagatagga gagcggtcct ggaccaacgg cctgctgcac
     1321 gccccacagc aaccattcgt aaagcggcgg gttgcgcagc tggtatccgc cggcgaggtg
     1381 tccttgcagg agctctgcgg cgagcgcgtc ctgcagggtc cgcccaggag acaccgccca
     1441 gagcatcaac acgaaggcca aggaccagag cagccagaag gccgccaccc cccgtgtcgt
     1501 cagcatttgg ccggcaagct tcgccatcca accctcactc gtccgcccgc ggcctttata
     1561 cacggcggca gacaagcgtc ccgcgcttgc ctataagggc gcccatgccg aaggtccccg
     1621 cccaaaaaac tccgccacga agtggcccac cgagaattgg ttttgtcagc ctgggctgcc
     1681 ccaaggcgct ggtcgattca gagcgcatca tcaccaagct gcgcgccgag ggctacgagc
     1741 tgtgttcgag ctatgagggc gccgacgccg tggtggtcaa cacttgcggc tttctgaata
     1801 gcgccaagga ggagtcgctt gcggcgatcg gcgaagcctt ggccgagaac ggcaaggtca
     1861 tcgtcaccgg ctgccttggc gtcgagtccg accgcatccg cgcggtgcat ccaggcgtgc
     1921 tcgccatcac cggcccgcat caatatgagg cggtagtgga ggaggtgcac aaggtggtgc
     1981 cccgcccgca cgatcccttc ctcgatttgc tgccgccgca aggcatcagg ctcacgccgc
     2041 gccactacgc ctatttgaag atctcggagg gctgcaacaa ccgatgcagc ttctgcatca
     2101 tcccgcattt gcgtgggcgg ctcgcaagcc ggccgatcgg cgatgtaatg cgcgaggccg
     2161 agaggctggt cgatgccggc gtcaaggagc tgctcgtcat ctcgcaggac acgagcgcct
     2221 atggtctcga tctcggctac gcacgaagca aatggcgcgg cgaggagcgc gaggcgcggt
     2281 tcctgagcct ggcagaagcg ctcggcagcc tcggcgcctg ggtgcggctg cactacgtct
     2341 atccctatcc ccatgtcgat caagttctgc cgctgatggc ggacgaaaag gtgctgcctt
     2401 atctcgacat cccgtttcaa catgcgagtc ccacggtgct taaggccatg cggcgaccgg
     2461 cgcatcagga gaaaactctg gaacgcatca gctcctggcg tgagacctgc cccgatcttg
     2521 ccatccgctc gaccttcatc gtcggcttcc ctggcgagac cgaggaggac ttcgactttc
     2581 tccttgagtg gctttcggaa gcgcggctcg cccgcgtcgg ctgcttcaaa tatgaagacg
     2641 tcgacggcgc cgcggccaat gcgcttcccg gccaggtgcc gcaagaagtg aaggaggagc
     2701 gttacgcgcg gctgatgcag catcagcagg cgatcagcgc cgagggtctg gccgaacgcg
     2761 tcggcaagac catcgagatc atcgtcgatg aggtcgacag caacggagcc atcgcccgtt
     2821 ccacctggga cgcgcctcag atcgacggga atgtctacct caacggcgag acgggcttga
     2881 gccccggcga tcgcgcgcgc gtcaccgtcg aagggtccga cgaatacgat ctgtggggca
     2941 cgctcgccga gaattccgcc gcgcaaacgc cggagcccgc ccgcg
//
LOCUS       DATNAE010000262         5797 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3866742, whole genome shotgun sequence.
ACCESSION   DATNAE010000262 DATNAE010000000
VERSION     DATNAE010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5797
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3866742"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1086
                     /locus_tag="VN518_05885"
     CDS             <1..1086
                     /locus_tag="VN518_05885"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXE04138.1"
                     /translation="ENLNAVLHYHDCQAEDARLVLAVLLDARARGADILNHRAVTAIE
                     PLANGYTIELDERGAKRRVDARFIVNAAGPFVAGVDALSSVAPPPRPLRLIRGSHIVL
                     RMPEPVEADAYTLQDKEDRVIFVMPWLDRRFLMVGTTEVPEKGDPGVAACSAEEQAYL
                     LDAYNRYFAPGSGPVTAADVVWSFAGVRALQDHGGRKPSRLTRRPALASIANGSGGFV
                     TLYGGKLTTHRAFAETVLDELRDLGAYMGGPWTKDVPLYGGGESRATLLSLVAQGPAS
                     ISRETRHRWAFTYGDQIERLYAKLLIEPNLAQEIAPGLTRVELIHAVETEDVMTAEDF
                     LLRRTRLHLLLDQGGRDAVARWFATDN"
     gene            1162..1416
                     /locus_tag="VN518_05890"
     CDS             1162..1416
                     /locus_tag="VN518_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE04139.1"
                     /translation="MAIYTVRKGKWYIARISLSGVNRFATNGMVAAKLEDAGFTSVAV
                     EGSGGIRVAIGYWPLDDATAEQPDEIVDIVEKDSEPEIPT"
     gene            complement(1420..1860)
                     /locus_tag="VN518_05895"
     CDS             complement(1420..1860)
                     /locus_tag="VN518_05895"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HXE04140.1"
                     /translation="MSGLFTLGPWTWMILAAILFALEVMSPGIFLMWFGMAAGVTGLL
                     AFGFDISWQWQLVWFCLLSLAAVLVALKYLRSNPLHSDRPLLNERAVQHIGQCYDLID
                     PIVNGRGSVKIGDSIWRVEGPELPKGARVRVLGADGTLLKVAPA"
     gene            complement(1873..2877)
                     /locus_tag="VN518_05900"
     CDS             complement(1873..2877)
                     /locus_tag="VN518_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010660672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HXE04141.1"
                     /translation="MLGFNIFVLVLLVLFVAVLAAGVKVVPQGWNYTVERFGRYTRTL
                     RPGLSLIIPFIDRIGRKLNVMEQVVEVPSQEVITKDNAMVKVDGIAFFQVIDAAQASY
                     EVTHLEIAILNLTMTNIRTVMGSMVLDELLSQRDEINHRLLKVVDDATSNWGVKVTRI
                     EIKDIEPPRDLIDSMARQMKAERDKRATILVAEGERQAAILTAEGAKQAQILAAEGRR
                     EAAYRDAEARERSAEAEAKATEVVSDAISKGNVQAINYFVANNYVGALKALAEAKNQK
                     VLILPIEATSVIGAIAGVAEIAREAFGDRGGNSASPPAPSKQPPTTTPRVRPGGVPSV
                     "
     gene            complement(2995..3468)
                     /locus_tag="VN518_05905"
     CDS             complement(2995..3468)
                     /locus_tag="VN518_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE04142.1"
                     /translation="MRAIGRAFIIFSFAMALGLGPVAMSAEPDAAKLDAAKPEAKPAA
                     PLAYISVAGAPGDGEQALAAALGKRLSAAGVKTATSFAANVYSVEGIVKVAAVKGGRQ
                     SVRIDWTIFGPDGNTLGGVSQTRIVRKGSLDRKWGPTADAAARAAAWEISKLISP"
     gene            complement(3497..>5797)
                     /locus_tag="VN518_05910"
     CDS             complement(3497..>5797)
                     /locus_tag="VN518_05910"
                     /inference="COORDINATES: protein motif:HMM:NF024298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE04143.1"
                     /translation="RITDIARDVRREVRELAASVGHVQTPAYYDEVVGDFCPAGCEAK
                     TAAGTSQITPAEVPRPSAEPKSAEAPATPSSPPVQTAATLTPQAVQGPAVGHAPVREC
                     DRLAADPDDPDSVVKGVEIGDTINAARAIPACEEAVRLYPQERRFSFQLARAQAHANH
                     PELAKPVLQALDKDGYAIAGVYLGFLYASGTGVAKDEAEAVRLFREGADAGVSLGALA
                     LAQAYKDGSGVAKDEAEALRWLRKSANAGSARAMTYLADQYADGTLGLPKDGAEAVSW
                     YRKAADLGSARAVYDLALMFQEGRGVPKDAAEAARLFQAGDDLKNGSAMVHVGLTYDI
                     GQRVTKDEAESLRWYRKAADLGDAEGMSFVAMAYQFGNGIAKEQAEAARWYRQAAELG
                     DMSAMTTLAKLYEKGVGVPQDAAEAKRWRDAAGTGSAPTGTAAPPSEDAKDDGKVAAA
                     SPSEPPASSSAVRGDLVRSFGPTEHEFISLVFSPDGGQLLALASGGYFMHLDPVSGAI
                     GRRLGDATSERPCCQLVISPDGKRVASGSFSNLTMLWDAESGELVRSFKGHTDHVVVV
                     ALSPDGKMLASGSQDQTIKLWDVESGELLRSLTGNDGKVASVSFSPDGGTLASGFNTG
                     AVKLWDVASGKLLKTIKNNNYSVDSLAFSPDGTLLAAKGYFEMVLWDVAKGQALRTLE
                     GEPDPLIFNCMAISPDGTRLASGDFEHSTRLWDVATGTLLKSFEGHAEGVRAVAFSPD
                     GNMLASASGDKTVKLWNLGSIEAAGR"
BASE COUNT         1023 a         1921 c         1802 g         1051 t
ORIGIN      
        1 gaaaatctca acgccgtgct gcactatcac gactgccagg ccgaagatgc ccgtctcgtg
       61 ctcgccgtgc tcctcgatgc gcgcgcccgc ggcgccgaca ttctcaacca ccgcgcggtg
      121 accgccatcg agccgctcgc caacggctac acgatcgagc tcgacgagcg cggggcgaag
      181 cgccgcgtcg atgcgcgctt catcgtcaat gccgcgggcc ccttcgttgc cggggtcgac
      241 gccctgagca gcgtggcgcc tccgccccgg cccttgcggc tgatccgcgg cagccacatc
      301 gtgctccgaa tgcctgagcc ggtcgaggcc gacgcctaca ccttgcagga caaggaagac
      361 cgcgtgatct tcgtgatgcc gtggctcgac cggcgtttcc tcatggtcgg caccaccgag
      421 gtgcctgaaa agggcgatcc aggcgtggcg gcctgctcgg ccgaggagca ggcctatctg
      481 ctcgacgcct ataaccggta ctttgcgcct gggagcggac ctgtcactgc cgccgatgtg
      541 gtgtggagct ttgccggcgt ccgcgcccta caggatcatg gcgggaggaa gccgagccgg
      601 ctcacccgcc gcccggcgct cgccagcatc gccaacggca gcggcggctt cgtcaccctt
      661 tatggcggca agctcaccac ccatcgcgcc ttcgccgaga ctgtgctcga cgagctccgc
      721 gacctcggcg cttacatggg cggcccgtgg accaaggacg ttccccttta tggcggcggc
      781 gaatctcgcg cgacacttct ctcccttgtt gcccaaggcc cggcgtccat ctcccgtgag
      841 acccgccacc gctgggcctt cacctacggg gaccagatcg agaggctcta tgccaagctg
      901 ttgatcgagc cgaacctcgc gcaagagatc gccccaggcc tcacgcgcgt cgagctgatc
      961 cacgccgtcg agactgaaga tgtcatgacg gctgaggatt tcctgctccg ccgcaccagg
     1021 ctgcacctct tgctggatca gggagggaga gacgccgtcg caaggtggtt cgcgaccgac
     1081 aattgagccc cggttgcgcc tgcaactact gacctttagt actgcgaata caactgtaag
     1141 acgcagaggg agggcggctt catggccatc tacacggtgc gtaagggcaa gtggtacatc
     1201 gccagaatct cgctctccgg agtcaaccgg ttcgccacta acggcatggt cgccgccaag
     1261 ctcgaggatg ccggcttcac cagtgttgcg gtcgaaggct caggagggat cagggtcgcc
     1321 atcggctatt ggcctctcga tgacgcgacc gcagagcagc cagacgagat cgtcgacatc
     1381 gtggagaagg acagcgagcc ggaaataccg acctagctct caggccggcg ccactttcag
     1441 caacgtcccg tcggcgccga gcacgcgcac ccgcgctccc ttcggcagct caggcccctc
     1501 cacccgccag atcgagtcgc cgatcttgac gctgccgcgt ccgttcacga tcgggtcgat
     1561 gagatcgtag cactggccga tatgctgcac cgcgcgctcg ttgagcagcg ggcggtcgct
     1621 gtgcagcggg ttgctgcgca gatatttcag cgccacgagc acggccgcga gcgacaacag
     1681 gcagaaccac acgagctgcc actgccagct gatgtcgaat ccgaaggcga ggaggccggt
     1741 gacgccggcg gccattccga accacatcag gaagatgccg ggcgacatca cctcgagcgc
     1801 gaacaggatc gcggcgagga tcatccaggt ccacggacca agcgtgaaga gaccgctcat
     1861 cgtggcggct ccttacacgg aaggaacgcc gcccggacgg acgcgcggcg tcgtggtcgg
     1921 aggctgcttg gacggggcag gcggcgaggc tgagttgccg ccgcggtcgc cgaaggcttc
     1981 gcgcgcaatc tcggccacgc cggcgatggc tccgatcacc gaggtcgcct cgatcggcag
     2041 gatcagcacc ttctgattct tggcctcggc cagagccttc agcgcgccga cataattgtt
     2101 ggcgacgaaa tagttgatcg cctgcacatt gcctttgctg attgcatcgc tgactacctc
     2161 ggtcgccttg gcttccgctt ctgccgaacg ctcgcgcgcc tcggcatcgc gataggcagc
     2221 ctcgcggcgc ccttccgcgg cgagaatctg cgcctgcttg gcgccttcag ccgtcaggat
     2281 cgccgcctgc ctctcgcctt cggcgacaag gatcgtcgcg cgcttgtcgc gctcggcctt
     2341 catctgccgc gccatcgagt cgatcaaatc gcgcggcggc tcgatgtcct tgatttcgat
     2401 gcgcgtgact ttgacccccc aattggaggt ggcatcgtcg accaccttca acaggcgatg
     2461 attgatctcg tcgcgttggg agaggagctc gtccagaacc atggagccca tcaccgtgcg
     2521 gatattggtc atggtgaggt tgaggatggc gatctccaga tgcgtcacct cgtagctcgc
     2581 ctgcgccgcg tcgatcacct ggaagaaggc gatgccgtca accttcacca tggcgttgtc
     2641 cttggtgatc acctcctggc tcggcacctc gaccacctgc tccatcacgt tgagcttgcg
     2701 cccgatgcga tcgatgaagg gaatgatgag actgaggccg ggccgaagcg tgcgcgtgta
     2761 gcggccgaag cgctcgaccg tgtagttcca accttgcggc acgaccttga cgcccgccgc
     2821 gagcacggcg acgaacagaa cgagcagaac gaggacgaag atattgaagc cgagcatggc
     2881 accctcctag aggtgtctgg cgagcgcgtg acggttagct atagcactca ccttccatcg
     2941 gcagccgata gatgggtgcg caccctcccg aatttaagag catcccgccg gccatcatgg
     3001 cgaaatcagc ttggagatct cccacgcggc ggcacgggcg gcggcatcgg cggtaggtcc
     3061 ccattttctg tcgagcgagc ctttgcgcac gatccttgtt tgtgacaccc cgccaagagt
     3121 gttgccgtcg gggccgaaga tcgtccagtc gatgcgcacg ctctgccgtc ctcccttgac
     3181 cgccgccacc ttgacgatgc cttccaccga atagacgttg gcggcaaatg acgttgcggt
     3241 cttgacgccc gcggcggaca acctcttgcc gagcgccgcg gcgagtgcct gttcgccatc
     3301 gccaggcgcg cctgcaactg agatgtaggc gagcggcgcc gcgggctttg cctcaggctt
     3361 ggcagcatca agcttggcag catccggctc ggcgctcatg gcgaccggac cgagccctaa
     3421 cgccattgcg aagctgaaga tgatgaaggc gcgaccgatc gctctcattc aaggtgccga
     3481 gcttcagccc accggctcag cggccggcgg cttcgatgct ccccagattc cagagcttga
     3541 ccgtcttgtc tccgctggcg gacgcaagca tgttgccgtc gggcgaaaag gcgacggcgc
     3601 gaactccctc cgcatgtcct tcgaagctct tgagcagcgt tcccgtcgcc acgtcccaga
     3661 gcctggtaga gtgctcgaaa tctccggacg cgagccttgt cccgtcgggc gaaatggcca
     3721 tgcagttgaa aatgagcggg tcaggctcgc cctctagcgt acgcaaggcc tgccccttgg
     3781 cgacgtccca gaggaccatt tcaaagtagc ctttggcggc gagcaaggta ccgtctggcg
     3841 agaaggccaa ggagtcgacg ctgtaattat tgttcttgat cgtcttcagc agcttgccac
     3901 ttgcgacgtc ccacagctta accgcgccgg tattgaaccc cgaggcgagc gtgccgccat
     3961 ccggagagaa ggatactgaa gcgactttgc cgtcattacc agtcaagctg cgcaagagtt
     4021 caccactctc cacgtcccac agctttatcg tctggtcctg acttccagag gcaagcatct
     4081 tgccgtcggg tgagagagcc acgaccacga cgtgatcggt atgccctttg aagcttcgga
     4141 ccagctcgcc gctttcagca tcccaaagca tggtcaagtt tgagaagctg ccggaggcca
     4201 cgcgcttccc atcaggcgaa atcacgagct gacaacaagg cctttcagaa gttgcatcac
     4261 cgagtctgcg cccgatagcg ccgctgacgg ggtcgaggtg catgaagtat cccccggagg
     4321 caagcgcgag caattgcccg ccatcggggg agaagactaa cgaaatgaat tcatgctcgg
     4381 ttggaccaaa gctgcggaca aggtcccctc tcacagcgct ggatgaagca gggggttcgc
     4441 tcgggctagc ggcggcgacc ttcccgtcat ccttggcatc ctccgacggc ggcgcggcgg
     4501 tgcctgtagg cgcggaaccc gtcccggcgg cgtcccgcca gcgtttcgct tcagccgcgt
     4561 cctgaggaac gcccacgccc ttctcgtaca acttggcgag agtcgtcatc gccgacatat
     4621 cgccgagctc ggccgcctgg cgataccagc gagcggcctc ggcttgctcc ttggcaatgc
     4681 cattgccgaa ttgatacgcc atagcgacga acgacatccc ctcggcatcg ccaagatcgg
     4741 ccgccttgcg ataccatcgc agggactcgg cttcgtcctt ggtcacgcgc tggccgatat
     4801 cgtaggtcag gccgacgtga accatcgccg agccgttttt cagatcatcg cccgcctgga
     4861 acagccgcgc tgcctccgct gcatccttgg gcacgccgcg gccttcttga aacatcagcg
     4921 ccaggtcgta cacagctcgt gcgctgccga gatcggcggc cttgcgatac caggacacgg
     4981 cttccgcgcc gtcctttggc agcccaagcg tgccgtcggc atattggtcg gcaaggtagg
     5041 tcatggccct ggcactgccg gcattcgcgg acttacggag ccagcggagc gcctcggctt
     5101 catccttggc aacgccgctt ccgtccttgt aggcttgcgc aagcgcaagc gctccaagcg
     5161 aaacaccggc atcggcaccc tcgcggaaga gacgcaccgc ctccgcttcg tccttggcca
     5221 cgccagttcc acttgcatat aggaaaccaa gatagacgcc ggctatggcg tagccgtctt
     5281 tgtcgagggc ttgcaggaca ggcttggcga gctccggatg attggcgtgg gcttgcgcgc
     5341 gggcgagttg gaatgagaag cggcgctctt gcggatagag gcggactgcc tcctcgcagg
     5401 ccggaatagc tcgcgcagca ttgatggtgt cgccgatctc gacgccctta accaccgagt
     5461 cggggtcatc tgggtcggcg gcgagccggt cgcattcacg gaccggcgca tgtccgaccg
     5521 ccggtccttg cacggcttgc ggggtgagtg tcgcggcggt ctggaccggc gggctgctcg
     5581 gcgtcgccgg ggcttcggcc gactttggtt cggctgatgg ccgagggact tcggcgggtg
     5641 ttatttggga cgtcccagcc gcggtcttcg cctcgcatcc ggcggggcaa aaatcgccga
     5701 ccacctcgtc gtagtaagca ggcgtctgca cgtggccgac gctcgccgcc aactccctca
     5761 cctcgcggcg cacatcgcgg gcgatatcgg tgatcct
//
LOCUS       DATNAE010000263         4907 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_1168804, whole genome shotgun sequence.
ACCESSION   DATNAE010000263 DATNAE010000000
VERSION     DATNAE010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4907
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1168804"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1305
                     /locus_tag="VN518_05915"
     CDS             <1..1305
                     /locus_tag="VN518_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012317466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HXE04144.1"
                     /translation="FFIEYFAIDLIMDDEGRCRGVVALSMQDGTLHRFRAHKVILATG
                     GYGRAYFSCTSAHICTGDGNAMVLRAGLPLQDMEFVQFHPTGVYGAGVLITEGARGEG
                     GFLTNSEGERFMERYAPNAKDLASRDVVSRAITIEIREGRGTGPDKDHMHLHLSHLDE
                     ALLAERLPGITESARIFAGVDLTREPIPIIPTAHYNMGGIPTNVHGEVVCPRNGDPDA
                     TVPGLFAVGEAACASVHGANRLGSNSLIDLVVFGRAAARRSAETIKRDSAMPELPKGA
                     GEEAIARLDRFRYASGGTPTAALRLKMQKTMQTDCAVFRTAPVMQQGVRKIHDVWKES
                     DDIRVTDRSLIWNTDLIETLEFDNLISQAAVTVAGALARQESRGAHAREDFPKRDDAN
                     WMKHTLSWADYDTRGVRLDYRPVHTYTLSNEIAYIEPKERVY"
     gene            complement(1338..1718)
                     /locus_tag="VN518_05920"
     CDS             complement(1338..1718)
                     /locus_tag="VN518_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXE04145.1"
                     /translation="MGALVPRILVVDDEPLISMLVENWLGELGCEVVGPARTVADGLA
                     LADGQLDGAILDVNLAGGTCYPLANALRSRGVPLAFATGDGGLDAGEGFEDPILLPKP
                     FVFENVKETLEALLEQSRKARLAS"
     gene            1827..3737
                     /locus_tag="VN518_05925"
     CDS             1827..3737
                     /locus_tag="VN518_05925"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HXE04146.1"
                     /translation="MLKASRLVQNAWIIMAVGFGLVVVAAIAVALMFARSREADRLVD
                     HTFEVQSTAQVLLSQVQAAENGQRGFLITGDDDYLKRFDGALARVPELLASLRQLTAD
                     NPRQQARLDRLEPLVKARFDRIQSSIDLAKEGDKDAAFAVVKAGEGKTLMDDIVAELS
                     AFVKAERDLLAMRQSKSAEIRLWLSLLTGMALVAATLLAVLLAIATRQAVSGLLDRTR
                     ELEEESKLRREAEATLGQAVKMEAVGQLSGGIAHDFNNLLTIIIGNLDAMKRQLAKLT
                     GLEPAPEIAGKLSKSLDAALRGAQSSAQLTQRLLAFSRRQALEPQRLDLNRLVSGMLE
                     MFRRTLGSDISIETVLGAGLWPTFADGHQLENVLLNLALNAKAAMPDGGCLTIETANT
                     YLDDAYVRRFGDIKAGQYAVLCVTDTGTGIAKDILDKVFEPFFTTKPPGEGSGLGLAM
                     VHGFVKQSGGHVRIYSEEGRGTTVKIYLPRLIGEEVGAVPAGKAEGEQAIPRAKPGEM
                     ILLVEDSEGVREYARDILLELGYGVIDAANGQEALRAVAKKPHISLLFTDVVLGESNG
                     RVLADKVRQIYPNLPVLFTTGYTRNAIVHQGRLDPDVQLLNKPFTQQDLARKLRELLD
                     AAPRPGLAAAAS"
     gene            3791..4579
                     /locus_tag="VN518_05930"
     CDS             3791..4579
                     /locus_tag="VN518_05930"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003531162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HXE04147.1"
                     /translation="MVEFTLPKNSKVQPGKAWNPPPGGAEAGRWREYSIYRFDPDKAE
                     NPRLDTYWVDMKECGPMVLDALIWIKNNIDPTLTFRRSCREGVCGSCSMNIGGTNTLA
                     CTLHADDAPERISIYPLPHLPVIKDLVPDLTHFYAQHRSIEPWLKTDTPTPQKEWRQS
                     REDRAKLDGLYECILCACCSTACPSYWWNGERYLGPAILLQADRWVEDSRDERTGERL
                     DELEDPFRLYRCHTILNCTKACPKGLNPAAAISSLKRQMVERGE"
     gene            4576..>4907
                     /locus_tag="VN518_05935"
     CDS             4576..>4907
                     /locus_tag="VN518_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXE04148.1"
                     /translation="MIDHIGLHVRDVARATEFYLEALSPLGYGIVMQVSAEETGHGAA
                     VGFGAGLKPSFWIGEGEPKSGPMHVAFVAPSRAAVDVFYAAAMKAGGKDNGPPGLRPE
                     YHANYYGA"
BASE COUNT          905 a         1615 c         1542 g          845 t
ORIGIN      
        1 ttcttcatcg aatatttcgc catcgatctg atcatggacg atgaggggcg ctgccgcggc
       61 gtggtggcgc tctccatgca ggacggcacg ctgcaccgtt ttcgcgccca taaggtgatc
      121 ctcgccaccg gcggctatgg ccgcgcctat ttctcctgca cgtccgcgca tatctgcacc
      181 ggcgacggca acgccatggt cttgcgcgcc gggctcccgc tccaggacat ggagttcgtg
      241 caattccatc caaccggtgt ctatggcgcc ggcgtgctca tcaccgaagg cgcgcgcggc
      301 gaaggcggct tcctcaccaa ttccgaaggc gagcgcttca tggagcgtta tgcgccgaac
      361 gccaaggatc tcgcctcgcg cgacgtggtc tcgcgcgcca tcaccatcga gatccgcgaa
      421 ggccgcggca ccggccccga caaggatcac atgcatttgc atttgagtca tctcgacgag
      481 gccctgcttg ccgagcggct gcctggcatc accgagtcgg cgcgcatctt cgccggcgtc
      541 gacctcaccc gcgagccgat cccgatcatc ccgacggccc actacaatat gggcggcatt
      601 cccaccaatg tgcatggcga ggtggtgtgc ccgcggaacg gcgaccctga cgcaacggtg
      661 ccgggactgt tcgccgtcgg cgaagcggct tgcgcttcgg tgcatggagc aaaccggctt
      721 ggctcaaact cgttgatcga tctcgtcgtg ttcgggcggg cggctgcgcg ccgctcggcc
      781 gagacaatca agcgcgacag cgccatgcct gagcttccga agggcgccgg cgaagaggcg
      841 atcgcgcggc tcgaccgctt ccgctatgcg agtggcggca cgccgaccgc ggcgctgcgc
      901 ttgaagatgc agaagaccat gcagacggac tgcgccgtgt ttcgcaccgc cccagtgatg
      961 cagcaggggg tgcgcaagat ccacgacgtg tggaaggaga gcgacgacat tcgcgtcaca
     1021 gaccgctcgc tgatctggaa caccgacctg atcgagacgc tcgaattcga caatctgata
     1081 tcgcaggcgg cggtcaccgt ggccggagcg ctggcgcggc aagagagtcg gggcgcgcat
     1141 gcgcgcgagg acttccccaa gcgcgatgat gcgaactgga tgaagcacac tttgtcgtgg
     1201 gcggattacg atacgcgcgg cgtccgcctc gactaccgcc cggtgcacac ttacacgctc
     1261 tccaacgaga tcgcttacat cgagccgaaa gagcgggtgt actaaatgtg tcattgccgg
     1321 ccttgtgccg gcaatcccta gcttgccagc cgtgccttgc ggctctgctc gagcaaggct
     1381 tcgagcgttt ccttcacgtt ctcgaaaacg aagggcttgg ggagcagtat cgggtcctcg
     1441 aagccctcgc ccgcgtccag cccgccgtcg ccggtggcga aagcgagagg cacgccgcgc
     1501 gagcgaaggg cgttggcaag cggatagcag gtaccgccgg caaggttcac gtcgaggatg
     1561 gcgccgtcca gctgcccgtc ggcgagcgcg agcccgtcgg caaccgtccg ggccgggcct
     1621 acgacttcgc agccaagctc gccgagccaa ttctcaacca gcattgagat cagaggttcg
     1681 tcgtcgacga ccaggatgcg cggcacgagg gctcccataa aatgcttgct tggacttggc
     1741 ctcgcggcaa gtgtagtggg aaagcgcaag acccgccaat atcttacgtt ccggttttcg
     1801 caagaggatc agccctgccg acgcgagtgc taaaagccag ccgcctggtg caaaacgcct
     1861 ggatcatcat ggccgtgggc ttcggcttgg tggtggtcgc ggccatcgcg gtcgcgctga
     1921 tgtttgcccg cagccgggag gccgacaggc tcgtcgacca caccttcgag gtgcaaagca
     1981 cggcccaggt cctgctcagc caagtccagg ccgcggaaaa cggccagcgc ggcttcctca
     2041 tcaccggaga cgacgactat ctcaagcgct tcgacggtgc gctcgcccgc gtgcccgagc
     2101 tgttggcaag cctgcgtcag ctgactgccg acaatccacg ccagcaggcc cggttggata
     2161 ggttagaacc gctggtcaag gcgaggttcg accgcattca aagctcgatc gatctggcca
     2221 aagagggcga taaggacgcg gcgttcgccg tcgtcaaggc cggcgaaggc aaaactctga
     2281 tggatgacat cgtcgccgag ctctctgcct tcgtgaaggc ggagcgcgat ctcttggcga
     2341 tgcgccagag caaatcggca gagatccggc tgtggctttc gctgcttacc ggcatggcgc
     2401 tcgttgccgc cacgctgctc gccgttctgc tcgccatcgc cacacggcaa gcggtcagcg
     2461 gcctgctaga tcgaacccgc gagctcgagg aagaatcgaa gctccgccgc gaggcggagg
     2521 cgaccctcgg ccaagcggtg aagatggagg ccgtgggtca gctcagcggc ggcatcgcgc
     2581 acgacttcaa caatctgctc accatcatca tcggcaatct cgacgcgatg aagcgccagc
     2641 ttgccaagct taccgggctc gaaccggcgc ctgagattgc cggcaaacta tctaagtcgc
     2701 tcgatgcggc tttgcgcggc gcgcaaagct cggcgcagct cacgcagagg ctgcttgcct
     2761 tctcgcgccg tcaggcactt gagcctcagc gcctcgacct caatcgcttg gtttcaggca
     2821 tgctcgagat gttccgccgc acgcttggat ctgacatcag catcgagacc gtgctcggcg
     2881 ccggcctgtg gccgacgttt gccgacggcc atcagctcga gaacgttctg ctcaatcttg
     2941 cgctcaacgc caaagccgcc atgcctgacg gcggctgcct gaccatcgag acggccaaca
     3001 cctatctcga cgacgcctat gtgcgcaggt tcggcgacat caaggccggg cagtacgcgg
     3061 tgctctgcgt caccgatacc gggaccggga tcgcgaagga tatcctcgac aaggtgttcg
     3121 agccgttctt caccaccaag ccgcccgggg aggggtctgg gcttgggctt gccatggtgc
     3181 acggcttcgt caagcagtct ggcggacacg tgcgcatcta cagcgaggag gggcgcggca
     3241 ctacggtgaa aatctacctg ccgcgcctca tcggcgaaga ggtcggtgcc gttcctgccg
     3301 gcaaggcaga aggcgagcag gccataccgc gcgccaagcc gggtgaaatg atcctgctgg
     3361 tggaggacag cgagggggtg cgcgaatacg cgagagacat cctgctcgag ctcggctatg
     3421 gcgtgattga cgctgccaat gggcaggaag cgctccgcgc cgtggccaag aagccgcaca
     3481 tcagcctcct gttcaccgac gtcgttctcg gcgagtcgaa tgggcgggtg ctcgccgaca
     3541 aggtccggca gatctatccg aatttgcccg tcctgttcac caccggctac acccgcaatg
     3601 ccatcgtgca tcaagggcgc ctcgatcccg acgttcaact cttgaacaag cccttcaccc
     3661 agcaggacct cgcccgcaag ctgcgcgagc tgctcgacgc agcgccgagg cccggattgg
     3721 cggctgccgc gtcgtgagtc cttagcgcct gctggacaag gtcccgcgct tcaaactaca
     3781 acgttgagac atggttgaat tcactcttcc caagaactcc aaggtccaac ccggcaaggc
     3841 ctggaatccg ccgccagggg gtgcggaagc cggccgctgg cgcgaataca gcatctaccg
     3901 cttcgatccc gacaaggctg agaacccgcg gctcgacacc tattgggtcg acatgaagga
     3961 gtgcgggccg atggttctcg atgctctcat ctggatcaag aacaacatcg accccaccct
     4021 caccttccgc cgctcctgcc gcgaaggggt gtgcggctca tgctcgatga atatcggcgg
     4081 caccaacacg ctcgcctgca cgctgcatgc cgacgacgca cccgagcgga tctcgatcta
     4141 tcccttgccg cacctgccgg tgatcaagga cctcgtgccc gacctcaccc atttctatgc
     4201 ccagcaccgc tcgatcgagc cctggctcaa gaccgatacg ccgacgccgc agaaagagtg
     4261 gcggcagagc cgggaggacc gtgccaagct cgacgggctt tatgagtgca tcctgtgcgc
     4321 ctgctgctca acggcctgcc cgtcttattg gtggaacggc gagcgctatc tcggcccggc
     4381 catcctcttg caggctgatc gctgggtcga ggacagccgc gatgagcgga ccggcgagag
     4441 gctggatgag ctcgaagatc ccttcaggct ctatcgctgc cacaccatcc tcaactgcac
     4501 caaagcttgc cccaaaggac tgaacccggc ggcggccatc tcctcgctga agcggcagat
     4561 ggtggagcgc ggcgagtgat cgatcacatc ggcctgcatg tgcgcgacgt cgcccgcgcc
     4621 actgagttct atctcgaggc gctcagcccc ctcggctacg gcatcgtcat gcaggtgtcc
     4681 gcggaggaga ccggccatgg cgccgcggtc ggcttcggcg cgggcctgaa gccgtccttc
     4741 tggatcggcg agggcgagcc gaagagcggg ccgatgcacg tcgccttcgt cgcgccatcc
     4801 cgcgccgccg tcgatgtctt ctatgccgcg gcgatgaagg ccggcggcaa agacaatggt
     4861 ccgcccgggc ttaggcccga ataccacgcc aattattacg gggcctt
//
LOCUS       DATNAE010000264         2903 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3565625, whole genome shotgun sequence.
ACCESSION   DATNAE010000264 DATNAE010000000
VERSION     DATNAE010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3565625"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2270)
                     /locus_tag="VN518_05940"
     CDS             complement(<1..2270)
                     /locus_tag="VN518_05940"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HXE04149.1"
                     /translation="MTRHELNDVFIRTSFLQGANATYLAELYARYEEDPASLDPEWRA
                     FFSSLDDHRDDVIAGARGPSWAPSNGTALAPAPPRGALSQREALAAAQDTLGARQLIR
                     AYRTRGHLAAKLDPLELVTRREHPELKPKTYGFTKADYDRPIYLGGALGLEFGTLRQV
                     LNILKRTYAGHIGVEYMYISDPEQRAWIQDRIERRDVGFTANGKKAILKKLIEAESFE
                     RFLDFKYTGTKRFGLDGGESMVPALEQIIKRGGQLGVSEIVIGMPHRGRLNILANVMG
                     KPYRAIFNEFRGGSAHPDEVEGSGDVKYHLGASSDREFDENRVHLSLTANPSHLEIVD
                     PVVLGKVRAKQDQRKDDERKSVLPLLLHGDAAFAGQGVVAECLGLSGLRGHRTGGSLH
                     FIVNNQIGFTTAPRFSRSSPYPSDVGKMIDAPIFHVNGDDPEAVVHVTKIAIEFRQRF
                     GKPVVIDMFCYRRHGHNEADEPAFTQPLMYQRIAQHPGVVDVYANRLVEEGLLTREEI
                     DRMQADFRNFLEEEFSAGEAYRPNRADWLDGKWSGIGHAEEGARRGVTGVDLDLLKEV
                     GRKLTSIPKSFTPHKTIARIMAARRKMIDEGQGIDWATAESLAYGTLLVEGFPVRLSG
                     QDSERGTFSQRHAVLMDQVNEKKYTPLKHVARDQARFEVINSMLSEEAVLGFEYGYSL
                     AEPNALVLWEAQFGDFANGAQVVVDQFISSGERKWLRMSGLVLLLPHGYEGQGPEHSS
                     ARLERYLQLCAEDNWQ"
     gene            complement(2344..>2903)
                     /locus_tag="VN518_05945"
     CDS             complement(2344..>2903)
                     /locus_tag="VN518_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HXE04150.1"
                     /translation="VKRALDGSQTRLIGPNCPGIITPGQCKIGIMPGHIHQPGKVGIV
                     SRSGTLTYEAVAQTTAVELGQSTCIGIGGDPVNGTNFVDALSLFLGDDATEAIIMIGE
                     IGGSAEEDGAEFLIEAGNTKPVVGFVAGSTAPPGRRMGHAGAVISGGKGDAKHKIEAM
                     RDAGIVVANSPAALGTTLARLLNDR"
BASE COUNT          490 a          908 c          974 g          531 t
ORIGIN      
        1 atctgccagt tgtcctcggc gcagagctgg agatagcgct ccagccgcgc ggaggagtgc
       61 tccggtccct ggccctcata gccatgcggc agcagcagca ccaagcccga catgcgcagc
      121 catttccgct cgccggacga gatgaactga tcgaccacca cttgcgcgcc attagcgaag
      181 tcgccgaact gcgcctccca aaggaccagc gcgttcggct cggcgaggct gtagccatat
      241 tcgaagccga gcaccgcctc ctccgagagc atggagttga tcacctcgaa ccgcgcctga
      301 tcccgcgcga cgtgcttgag aggcgtgtat ttcttctcgt tcacctgatc catcagcacg
      361 gcatggcgct gcgagaaggt gccacgctcg gaatcctgtc ccgacaggcg caccgggaaa
      421 ccttcgacca gaagcgtgcc gtaggcgagc gattcggcgg tcgcccagtc gatgccttga
      481 ccctcgtcga tcatcttgcg gcgggcggcc atgatgcggg cgatggtctt gtgcggggta
      541 aagcttttcg ggatggaggt gagcttgcgg cccacctcct tcagcaagtc gagatcgacg
      601 ccggtcacgc cgcgccgcgc gccctcttcg gcatggccga taccggacca tttgccgtcg
      661 agccagtcgg cgcggttcgg acgataggcc tcgcccgcgg agaactcttc ctcgagaaaa
      721 ttgcggaaat ccgcctgcat gcggtcgatc tcctcgcggg tgaggagacc ctcctcgacc
      781 aggcggttgg catagacgtc gaccacgccc ggatgctggg cgatgcgctg atacatcagc
      841 ggctgggtga aggcgggctc gtcggcctcg ttatggccat ggcgccggta gcagaacatg
      901 tcgatgacga ccggcttgcc gaaccgctgg cggaactcga tggcgatctt ggtgacgtgc
      961 accacggcct ctgggtcgtc gccgttcacg tggaagatcg gcgcgtcgat catcttgccg
     1021 acgtccgagg ggtagggcga ggagcgcgaa aagcgcgggg cggtggtgaa accgatctga
     1081 ttgttgacga tgaaatgcag cgagccgccg gtgcggtggc cgcgcaagcc ggaaaggccg
     1141 aggcattccg ccaccacgcc ttgcccggcg aaggcggcat cgccatgcag caagagcggc
     1201 agcaccgatt tccgctcgtc gtccttgcgc tggtcctgct tggcgcgaac cttgccgagc
     1261 accaccggat cgacgatctc gagatgcgac gggttggcgg tgagcgagag atgcacgcgg
     1321 ttctcgtcga actcgcggtc cgacgaggcg ccgagatgat atttcacgtc gcccgagccc
     1381 tcgacctcgt cgggatgcgc cgagccgccc ctgaactcgt tgaagatcgc tcggtagggc
     1441 ttgcccatga cattggcaag gatgttcaag cgaccgcgat gcggcatgcc gatgacgatc
     1501 tcgctgacgc caagctggcc gccgcgcttg atgatctgct cgagcgccgg aaccatcgac
     1561 tcgccgccgt caagcccgaa gcgcttggtg ccggtatatt tgaagtcgag gaagcgctcg
     1621 aagctctcgg cttcgatcag cttcttgagg attgccttct tgccgttcgc cgtgaagccg
     1681 acgtcgcggc gttcgatgcg gtcctggatc caggcgcgct gctcggggtc ggagatgtac
     1741 atatattcga cgccgatatg accggcatag gtccgcttca ggatgttcag cacctggcgc
     1801 agcgtgccga attcgaggcc gagcgcgccg ccgagataga tagggcggtc gtagtcggct
     1861 ttggtgaagc cgtaggtctt gggcttgagc tccggatgct cgcggcgggt gacgagctcg
     1921 agcgggtcaa gcttggcggc gaggtggccg cgagtgcggt aggcgcggat caactgccgc
     1981 gcgcccaatg tgtcctgcgc ggcggcaagc gcctcacgct ggctcagcgc gccgcgcggc
     2041 ggcgccggcg ccagtgccgt tccgttcgag ggcgcccagg aggggccacg cgctccggcg
     2101 atgacgtcgt cccggtggtc gtcgagactc gagaagaacg cgcgccactc gggatcgagc
     2161 gaggccggat cctcttcata acgagcgtag agctcggcga ggtaggtggc gttggcccct
     2221 tgcaagaacg acgtgcggat aaacacgtcg ttcagttcgt gtcgcgtcat ttaagtcgac
     2281 cctgccggcc gttgaagggc caccggcgtg cccggcaaac aaggcgagct ttgggcgctc
     2341 gcctcacctg tcatttaata atcgtgcaag ggttgttcca agggcggcgg gggagtttgc
     2401 cacgacaatt cccgcatcac gcattgcctc aattttgtgc ttggcgtcgc ctttgccgcc
     2461 tgagatcacg gcacccgcat ggcccatgcg ccgaccgggc ggggcggtcg agcctgcgac
     2521 aaatccgact accggtttcg tgtttcccgc ttcgatcagg aactcggcgc cgtcctcctc
     2581 ggcgctgccg ccgatctcgc caatcatgat gatggcctcg gtggcgtcgt cgccgaggaa
     2641 cagcgacagc gcatcgacga agttcgtgcc gttgacgggg tcgccgccga tgccgatgca
     2701 agtcgattgg ccgagctcga cggcggtggt ctgcgccacc gcctcatagg tgagcgtacc
     2761 cgagcgcgag acgatgccga ccttgccggg ctggtggatg tggcccggca tgatgccgat
     2821 cttgcattga cccggcgtaa tgatgcccgg gcaattcggg ccgatcagcc gcgtttgcga
     2881 gccatcgaga gcgcgcttga cct
//
LOCUS       DATNAE010000265         2502 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_1459161, whole genome shotgun sequence.
ACCESSION   DATNAE010000265 DATNAE010000000
VERSION     DATNAE010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1459161"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1522)
                     /locus_tag="VN518_05950"
     CDS             complement(<1..1522)
                     /locus_tag="VN518_05950"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HXE04151.1"
                     /translation="MPPRSSLPFFSSSSDARAATLQGLLIATIVICALYFGREILLPL
                     ALAILLSFVLTRPLILLRRVKVPRLLAVGIVVGSAFVIIVALGWLLSREAADLATDLP
                     RYQTTLSVKIKSLRDSTASSPALQRAGNVLNNLQNELFGPDDGSSQPPPAPQVGTPAQ
                     KPDDKPMEVVVKERELTPLEVYQSIAGTVLPPLATAGIVLLIVVFILLQREDLRDRLI
                     RLFGSGDPQRATSTLTDAANRLSRYFLRQVLINSAYGALVAMALWAIGMPSPLAFGLL
                     AGLMRFVPYVGVFIAAAPPLFVAAAVDPGWTSFFAVLAIYAVGEFTMGQVVEPVVFGH
                     GTGVSPIAVVVSTVFWTWLWGPLGLILAMPMTVCLAVLGRHVEGLEFFDVLLGDQPAL
                     KPDESFYHRALAGDAAEITYQAELGLKNRSLQCYLDEVALPALRLAERDLARGSLDQE
                     QTEKIAATVNEMLDSLSDYEPRRWFAHLRRKPEHEKDEEAVTGLASLDAAEESEGEG"
     gene            complement(1791..>2502)
                     /locus_tag="VN518_05955"
     CDS             complement(1791..>2502)
                     /locus_tag="VN518_05955"
                     /inference="COORDINATES: protein motif:HMM:NF014345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HXE04152.1"
                     /translation="ATVDEVREGVKNIVLVPTPAPGLGSPQGAVPGAHFFLQDKSGKS
                     LVVEPVDGTLKVHDAPLGVMTNAPTYDWHMTNLANYINLSVKDVDSVKVGPTTVTAFG
                     SGAGLLGLPGDFTPPSRFVRAVVYSQSAAPSATADDAVLSAFHILNQFDIPKGAVMNS
                     AIGEPVAEITEWTSVADLKNLRWYFRTFTDQSIHMVDLKQAVDAAKGDIATIEMEGTK
                     QPINNVSSATAAVEQASK"
BASE COUNT          466 a          776 c          857 g          403 t
ORIGIN      
        1 caccctcacc ctcgctctcc tccgccgcat cgagcgaggc aagaccggtc accgcctctt
       61 cgtccttctc atgctcgggc ttgcgccgga gatgggcaaa ccagcggcgg ggctcgtagt
      121 ccgacaggga atcgagcatc tcattcaccg tggcggcgat cttctcggtc tgctcctggt
      181 cgagcgagcc gcgggctaga tcgcgctcgg cgagcctgag tgctgggagc gcgacctcgt
      241 cgagatagca ttggagactc cggttcttga ggccgagctc ggcttgatag gtgatctcgg
      301 ccgcgtcgcc cgcgagagcg cgatggtaga agctctcgtc gggcttgagc gcgggctgat
      361 cgccgagcag cacgtcgaag aactcgagcc cctcgacgtg gcggccgagc acggcgaggc
      421 agacggtcat gggcatggcg aggatcagcc caagcggtcc ccacagccac gtccagaaca
      481 cggtggagac gacgacggcg atcggcgaga cgccggtgcc gtgaccgaac acgactggct
      541 cgaccacttg gcccatggtg aactcgccca cggcatagat cgcgagcacg gcgaaaaagc
      601 tggtccatcc gggatcgacc gcagcggcaa cgaacagcgg cggagcggcg gcgatgaaca
      661 cgccgacata cgggacgaag cgcatcagcc cggcgagaag gccaaaggcg agcgggctcg
      721 gcatgccgat ggcccacagc gccatggcga cgagcgcgcc ataggccgag ttgatcaaca
      781 cctgtctcag gaaatagcgg ctcaggcggt tggccgcgtc ggtcaaggtc gacgtggcgc
      841 gctgcgggtc gcccgagccg aacagcctga tgagccgatc gcgcaagtcc tcgcgctgca
      901 gcaggatgaa caccacgatg agcagcacga tgccggcggt ggcgagcggc ggcaaaaccg
      961 tgccggcgat cgactgataa acttcgagcg gcgtcagctc gcgctcttta accaccactt
     1021 ccatcggctt gtcgtcgggc ttttgcgccg gagtgccgac ctggggggca ggcggcggct
     1081 gagagctccc gtcgtcggga ccgaacagct cgttctgcaa attgttgagc acattgcccg
     1141 ctctttgcag cgccggcgag cttgccgtgg aatcgcgcag actcttgatc ttcaccgaca
     1201 gcgtcgtctg gtagcgcggc aggtcggtgg caagatcggc agcctcgcgc gagagcagcc
     1261 agccgagcgc cacgatgatc acgaaggcgg agccgaccac gatgcccacc gcgagcaggc
     1321 gcggcacctt cacgcgccgg agcaggatga gcggccgggt gagaacgaag ctcaggagga
     1381 tggcgagcgc gagcggcaag agaatctcgc gcccgaaata gagagcgcag atgacgatgg
     1441 tcgcgatcag gaggccctgc agcgtcgcgg cgcgcgcgtc ggatgacgaa gagaagaaag
     1501 gcagggacga acggggcggc atatcgcgaa tctcccggcg ccggcgttcc tccggcatag
     1561 caagacgatc gaagctccga taggcccttc ggcttcggcg gatccgctta tccagggacc
     1621 gcgagccgca taacaagcct atggcagtat cgttcccttt cctccgcaag gaaaccaaga
     1681 ccccggggga atgtaccccc ggggtccctc ggccggcctt gaggcaccgt actcattact
     1741 gatcggcgcc agaggcctgc tttccgctca tgaaggcgtg gatagctcct ttatttggac
     1801 gcttgctcga cggcggcggt ggccgacgag acgttgttga tcggctgctt ggtgccctcc
     1861 atctcgatgg tggcgatgtc acccttggcg gcatcgaccg cctgcttgag atcgaccatg
     1921 tggatcgatt gatcggtgaa ggtgcggaaa taccagcgga gattcttgag gtcggcgacg
     1981 ctcgtccatt cggtgatctc ggcgaccggc tcgccgatgg ccgagttcat caccgcgcct
     2041 ttcgggatgt cgaactggtt gagaatgtgg aaggccgaca gcaccgcgtc gtcggccgtt
     2101 gcactcggcg ccgccgactg gctgtagacc acggcgcgca cgaagcgcga gggcggggtg
     2161 aaatcgccgg gaaggccgag caggcctgcg cccgacccga aggcggtgac ggtcgtgggt
     2221 ccgactttca cagagtcgac gtccttcact gagagattga tgtagttggc gagattggtc
     2281 atgtgccagt cataggtcgg cgcgttggtc atcacgccga gcggggcgtc atgaactttc
     2341 agcgtgccgt cgaccggctc gaccacgagc gacttgcccg acttgtcctg gagaaagaaa
     2401 tgcgcgccgg ggaccgcgcc ttgcgggctg ccgaggcccg gtgctggcgt cggcaccagc
     2461 acgatattct tcacgccctc gcgaacctcg tcgacggtgg cg
//
LOCUS       DATNAE010000266         7112 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_267606, whole genome shotgun sequence.
ACCESSION   DATNAE010000266 DATNAE010000000
VERSION     DATNAE010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 7112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7112
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267606"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VN518_05960"
     CDS             complement(<1..188)
                     /locus_tag="VN518_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HXE04153.1"
                     /translation="MRRGLDFKVTGVDCERAEPYLRAITASFGLATPYQLHLDEVIPA
                     HAGLGSGTQLALAIGSAVA"
     gene            440..826
                     /locus_tag="VN518_05965"
     CDS             440..826
                     /locus_tag="VN518_05965"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HXE04154.1"
                     /translation="MATSERTYTDAEVEERLKKELPHWYLEGGWIRRKYKTNSWKGTL
                     MVINAVGHLAEVAWHHPDITASYAWVEVRLMNHAAKGITDKDFALAKKIEEVVHWQPA
                     LEGGPLEGTPADDMRFAYIKHDKPKK"
     gene            1007..1915
                     /gene="fhcD"
                     /locus_tag="VN518_05970"
     CDS             1007..1915
                     /gene="fhcD"
                     /locus_tag="VN518_05970"
                     /EC_number="2.3.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran--tetrahydromethanopterin
                     N-formyltransferase"
                     /protein_id="HXE04155.1"
                     /translation="MANLKIKGVEIIETFAEAFPMVGTRIIITAPTQKWALIAAQTMT
                     GFATSVIACGAEAGIERLVPAEETLDGRPGVAVLLFTMDVNKLQTQLRNRVGQCVLTS
                     PGSACFAGLEGEKKLKLGSSLRFFGDGWQVSKLIGGRRFWRIPVMDGEFVCEQTTGLT
                     KKAVGGGNLLVLGRSYRAVLSACERAVKAIGGVADAIAPFPGGIVRSGSKVGSKYKGQ
                     IASTNDAYCPTLKAAVSTALPLNVEAVMEIVIDGLSAPAVAAAMRAGIYAAVKGGAGE
                     GITHITAGNYGGNLGPHHFHLKEIVA"
     gene            1912..2715
                     /locus_tag="VN518_05975"
     CDS             1912..2715
                     /locus_tag="VN518_05975"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018493 - formylmethanofuran dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit C"
                     /protein_id="HXE04156.1"
                     /translation="MSALTLELRSEPNQRLDLSPLTPTRLAGMKPKDIQAIAIGTARE
                     PVTVGDLFKLKGTDAVKLRFLNTDARCDKIGEGLTEGEIVVDGDAGAYLGSKMRAGSI
                     AVSGSAGALAGSSMRGGSIDIAREAGEKAGGVAVGEAHGMRGGRLSIGGKAGALLGER
                     MRRGLIIGRGGAGDYAGGRMIAGTILLKGRVGRFAGYGLRRGTLILDKQPKDLLPTFG
                     DCGVLDFTWLRLLDRQLKATDGRFRVGYRARRLMGDMAVLGKGEMLILA"
     gene            2799..4655
                     /locus_tag="VN518_05980"
     CDS             2799..4655
                     /locus_tag="VN518_05980"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:NF005698.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HXE04157.1"
                     /translation="MTATPFILLDDSLTATAPGAAGRTLLFERPKRVVAVYQQSEVEA
                     GLDAISEGLARGLHAAGFFAYELGYCLEPKLHALLPQDRQQPLFWIGLFREPVRFNDA
                     ETRRWLDANGALERSTISDLQLSWTREQYRSAFAKVQDYIAAGDVYQINLTLKYRFAF
                     TGDAVALYAALRRKQRVEYGALIDSGEFQVLSLSPELFFRREGKHVTSRPMKGTAPRG
                     RTPREDARLKTWLTVDEKQRAENLMIVDLLRNDLGRIAKIGSVEVTDLFTVETYRTVH
                     QMTSGITAELRADMGLKDMLKALFPCGSVTGAPKVRAMEIVRELEAYPRGVYTGAIGH
                     IAPNGDSQFNVAIRTLFVDSGGAGEMGIGSGVVSDSKVDAEFEECLLKAQFLTKADAP
                     FELIETIRWERGIGFHLLERHLVRLQSSATHFGYPFERGMVLAALSAEVEQLDGDVHM
                     VRLLLAEDGAITVTSTRIELPSKDTVWRFAISDQRVDEKDALFFHKTTRRQFYDREME
                     RQNALTGCDEVVFLNKKGELTEGTRTNLFIELDGRLFTPALPCGLLPGTLREELIDLP
                     RAAASEAILTLADLTVADRIYLGNSVRGLIRAEPLGLSRKSKTARELEPADS"
     gene            complement(4721..5143)
                     /locus_tag="VN518_05985"
     CDS             complement(4721..5143)
                     /locus_tag="VN518_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE04158.1"
                     /translation="MTCFLRNAFLALVAFATLAIIVMMAMTAEAAEAKDVKPATETAS
                     TADTARQIVVEEKAKPSDTVTPAKDEAAAEPAKTAEAPAVADGPAEAAAPAPEAPVAE
                     AAPAPKPQIVLLPRPKPYVTYVHRYSGYRYDGGYDHCD"
     gene            5343..>7112
                     /locus_tag="VN518_05990"
     CDS             5343..>7112
                     /locus_tag="VN518_05990"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HXE04159.1"
                     /translation="MPVLAALLLLLFTAVANAAEGPYLELDTKGHMALIRSIVFTPDR
                     SELISASDDKTIRVWDVGTGRIVRTIRGEIGDGDPGKIYALALSRDGRLLAAGGRTGA
                     TGQGQPIRLYDLNEGTLIGLLEGHDDAVLSLDFSPDGERLVSGSTDDTAIIWDVASRK
                     ALHRLVGHKGDVNAARFTADASGVVTASDDNTLKLWDARDGALVSTLTGHQDKVIALA
                     VSLRDGQVASGALDGTIKFWDLINGRVLKEINQGTEVMSLAFSPDGRRLLSGVGRAPY
                     LTHVWDAATGSEVASYGGHDNIVLATALAENGLAATAGGNDNEIHLWDAESGKLVRAL
                     VGVGRAIWSVGFSPDGRSIAWGVERPGSGELGSLAFSLKLPARDSPLGEPKALRAGAD
                     EFARAVTEAGGFSLQTRASGEFGYLDLLDVVKDGKVLATVKRGENAGYVHNAYTFTPD
                     GKTVISGGGHGFLTAYDLSGRTLGEFVGHTSDVWAVAVSPDGRWLLSGSDDQTVRLWS
                     MATHENVASLFYGKNGEWVLWTPQGYYAASPSGDAHVGWHVNEGEGKLARFVSAAQLK
                     SHFYRPDIVTRALVLGSAARAVKE"
BASE COUNT         1228 a         2333 c         2325 g         1226 t
ORIGIN      
        1 gcgacagccg acccgatggc aagcgcaagc tgcgtgccgg agccgagccc ggcatgcgcc
       61 gggatcacct cgtcgagatg cagttgatag ggcgtggcga gaccgaagct tgccgtgatc
      121 gcgcggagat agggctctgc gcgttcgcag tcgacgccgg taaccttgaa gtcgagcccg
      181 cgccgcaagc cgagccgcgt tcgcggccgg tcgatcgcaa gtccgaagct gccgaacggc
      241 cgcgtccccg cccgctcgga tcgaggaagc cgaaatgcag gcgcgcgctt gtgcttaccg
      301 aaacaaggtc gcgcgatcca gcaaccctgt cggcgcctgt gcacgcggga cgcaaggcct
      361 cctccattgt tggctcgaag cctctgggat atgcttggtg gtggaggaca gagtcgcact
      421 gtttcaaaag atcgccccga tggcaacttc tgagcgaact tacacggacg ccgaagtcga
      481 ggagcggctc aagaaagagc tgccgcattg gtacctcgag ggcggctgga tcaggcgcaa
      541 atacaagacc aatagctgga agggcacgct gatggtgatc aacgcagtcg gccaccttgc
      601 cgaggtcgcc tggcaccatc ccgacatcac tgcctcctat gcctgggttg aggtcaggtt
      661 gatgaaccac gccgccaagg gcatcacaga caaggacttt gcgctcgcca agaagatcga
      721 ggaggtcgtc cactggcagc cggcactcga aggcgggccg ctcgaaggca cgcccgccga
      781 cgacatgcgc ttcgcctata tcaagcacga caagccgaag aagtaaggct tcctgcggcc
      841 ctggtctgag aaccgaagcg gcccccgcgc aaattcgcgc gcttgcgcgc ctcccggcac
      901 ctgtttaatg gtgcgggccc atggcggtgt acttaaaggg ggagagggtc gacgagccaa
      961 gcgcgcccag gcgcgcggcc aaactgctcg gccacgcgcg ggtgccatgg cgaacctgaa
     1021 gatcaaaggc gtcgaaatca tcgagacctt tgccgaggcc ttccccatgg tcggcacgcg
     1081 catcatcatc accgcgccga cccagaaatg ggcgctgatc gccgcccaga ccatgacagg
     1141 ttttgccact tcggtgattg cttgcggggc cgaagccggc atcgagcggc tggttcccgc
     1201 cgaagagacg ctcgacggcc ggccaggcgt cgccgtgctg ctgttcacca tggacgtcaa
     1261 caaactgcag actcagctcc gtaaccgcgt cggccaatgc gtgttgacga gcccgggttc
     1321 ggcctgcttc gccgggctcg aaggcgagaa aaagctgaag ctcggctctt cgctccgttt
     1381 tttcggcgac ggctggcagg tatcgaaatt gatcggcggc cggcgcttct ggcgcatccc
     1441 ggtgatggac ggcgagttcg tctgcgagca gacgactggc ctcaccaaga aggcggtcgg
     1501 cggcggtaat ctgctcgtcc tcggccgctc gtatcgcgcc gtgctctcgg cgtgcgagcg
     1561 cgcggtgaag gcgatcggcg gcgtcgccga cgcgatcgct ccgttccccg gcggcatcgt
     1621 gcgctcgggc tccaaggtcg ggtccaaata caaaggccag atcgcctcga ccaacgacgc
     1681 ctattgcccg acgctcaagg ctgcggtgag tacggccttg cccttgaatg tcgaggcagt
     1741 gatggagatc gtcattgacg gcttgagcgc tcccgccgtt gccgcggcga tgcgcgccgg
     1801 catctatgcg gcggtcaaag gcggtgccgg cgaaggcatc acccacatca ccgccggcaa
     1861 ttacggcggc aatctcgggc cccatcattt ccacctcaag gagatcgtcg cgtgagtgcc
     1921 ttgacgctcg agcttcgcag cgagccgaac cagcggcttg acctgtcgcc gctgacgccg
     1981 acgcggctcg ccggcatgaa gcccaaggac atccaggcca tcgccattgg cacggcacgc
     2041 gagccggtca ccgttggcga tctgttcaag cttaagggca ccgacgccgt caagctccgc
     2101 ttcctcaaca ccgatgcacg gtgcgataag atcggggagg ggcttaccga gggcgagatc
     2161 gttgtcgacg gcgacgccgg ggcctatctc ggctcgaaga tgcgcgcggg cagtatcgcg
     2221 gtgagcggct cggcaggtgc gttggccgga tccagcatgc gcggcggctc gatcgacatt
     2281 gctcgtgaag ccggcgagaa ggcgggcggc gtggcggtgg gcgaggccca cggcatgcgc
     2341 ggcggcaggc tctcgatcgg cggcaaggcc ggcgcgcttc tcggcgagcg catgcgccgg
     2401 gggctgatca ttggccgcgg cggcgccggc gactatgccg gaggccgcat gatcgccgga
     2461 accattctgc tcaaaggccg cgtcggccgc tttgccggct acggcctgcg ccgcggcacg
     2521 ctgatcctcg acaagcagcc gaaggatttg ctgccgacct tcggcgattg cggggttctc
     2581 gatttcacct ggctccgcct gctcgaccgg cagctcaagg cgaccgacgg ccgcttcagg
     2641 gtcggctacc gcgccaggcg gctcatgggc gacatggccg tgctcggcaa gggcgagatg
     2701 ctgatcctgg cctaaaggcc aaatccctcg atgttgacct gcggcactcc catatgggtt
     2761 atgcttgcga gcttaagcgg atgcgaccgg ccgattgaat gaccgccacc cctttcattc
     2821 tcctcgacga cagcctgacc gcgaccgccc ccggtgcggc cggcaggacg ttgctgttcg
     2881 agaggcccaa acgggtcgtt gccgtctatc agcaaagcga ggttgaagcc gggctcgacg
     2941 ccatcagcga ggggctggcg cgcggcctcc atgccgctgg cttcttcgct tacgagcttg
     3001 gctactgtct cgagccgaag ctgcacgcgc tcctgcctca ggaccggcag cagccgctgt
     3061 tctggatcgg acttttccgc gagcctgtcc gcttcaacga tgccgagacg cgtcgctggc
     3121 tcgacgctaa tggcgcgctc gagcgctcga ccatctccga cctgcagctt tcctggacgc
     3181 gcgagcaata taggagcgcc tttgccaagg ttcaggacta catcgccgcg ggcgacgtct
     3241 atcagatcaa cctcacgctg aaataccgct tcgccttcac cggcgacgcc gtcgcgctct
     3301 acgcggcgct ccgccgcaag cagcgcgttg aatatggcgc gctgatcgac tcaggcgaat
     3361 tccaggtcct ctctctgtcg ccggagctgt tctttcgccg cgaaggcaag cacgtgacga
     3421 gccgcccgat gaagggcacc gccccgcgcg gtcgcacgcc gcgcgaggac gcccgcctca
     3481 agacctggct caccgtcgat gagaagcagc gcgccgagaa tttgatgatt gtcgatctcc
     3541 tgcgcaacga tctcggccgc atcgccaaga tcggctcggt cgaggtcacc gatcttttca
     3601 ccgtggaaac ctaccgcacc gtgcatcaga tgacctccgg catcaccgct gagcttcgtg
     3661 ccgatatggg gctcaaggac atgctgaagg cgctgttccc ctgtggctcg gtgaccgggg
     3721 cgccaaaggt ccgcgccatg gagattgtcc gtgagctcga ggcctatccg cgcggcgtct
     3781 acacgggcgc catcggccat atcgcgccga acggcgacag ccagttcaac gttgccatcc
     3841 gcaccctgtt tgtcgattcc ggcggtgccg gcgagatggg catcggctcc ggcgtcgtgt
     3901 ccgattcaaa ggtcgatgcc gaattcgagg aatgcctgtt gaaggcgcag ttcctcacca
     3961 aggccgatgc gccgttcgag ctgatcgaga ccatccgctg ggagcggggg atcggcttcc
     4021 atctgcttga gcggcatctc gtgcggctcc agtcctccgc cacccatttc ggctatccct
     4081 tcgagcgcgg catggtgctc gccgcgctgt cggccgaggt cgagcagctc gacggcgacg
     4141 tgcatatggt gcggctcttg ctcgccgagg acggcgccat caccgttacc tcgaccagga
     4201 tcgagcttcc ctccaaggac acggtgtggc ggttcgccat ctccgatcag cgcgtggacg
     4261 agaaggatgc gctgttcttc cataagacca cgcgccgcca gttctacgac cgcgagatgg
     4321 agcggcagaa tgccctgacc gggtgcgatg aggtggtgtt cctcaacaag aagggcgagc
     4381 tgaccgaggg cacccgcacc aatcttttca tcgagctcga cgggcgcctg ttcaccccgg
     4441 cgctcccttg cgggctgctc cccggcacct tgcgcgagga gctgatcgat ctgccgcgcg
     4501 ccgctgcgag cgaggcgatc ctgacccttg ccgatctcac cgtcgccgac cgcatctatc
     4561 tcggcaattc ggtgcgcggg ttgatccgcg ccgagcctct tgggctttcg cgcaagtcaa
     4621 agaccgcgcg cgagttggaa ccggcagact cctgagaacc gtcaccccta aacgtgaatg
     4681 gccgggacga gcccggccat tcacagtcag ttggaagcgg ctagtcgcag tgatcgtagc
     4741 cgccgtcata gcggtagccg ctgtaccggt gcacataggt gacatagggc ttcggtcttg
     4801 gcagcaacac aatctgcggc ttgggagccg gagccgcttc ggcgacaggg gcctcggggg
     4861 ccggcgcggc ggcctcggct gggccgtcgg caacggcggg ggcctcagcg gtcttggcag
     4921 gctcggctgc ggcctcgtcc ttggccggcg tcacggtgtc gctgggctta gccttttcct
     4981 cgaccacgat ttggcgcgcg gtgtcggcgg ttgaggcagt ttcggttgcg ggcttcacgt
     5041 cttttgcctc ggctgcttcg gccgtcatgg ccatcatcac gatgatggca agcgtggcaa
     5101 aggcgacgag cgccaggaaa gcattgcgca gaaagcatgt catggtccga cctccttcag
     5161 ggtcttggtg tcaatctcgc tcaaattcat ggacagtttc ttaggggagg gcgtctgaac
     5221 cgaacctgag ggcgccattc attttgcgtt cagcgcgcag gagcgatcta ctggagcgga
     5281 ggttcgcaaa gccaatgccc ctgcaagccc tgaaacgcca agccagatgc gagcgatggc
     5341 gcatgcccgt cttggccgcg ctcctccttt tgctgttcac cgccgtggca aacgcggctg
     5401 aagggccgta tttggagctc gacaccaagg gccacatggc cttgatccgc agcatcgtgt
     5461 tcacgccgga ccggagcgag ctgatctcgg ccagcgacga caagaccatc cgcgtctggg
     5521 acgttggcac cggccgcatc gtccgcacca tcagaggcga gatcggagac ggcgatccgg
     5581 gcaagatcta tgcgctcgcg ctctcccgcg acgggcgcct gcttgccgcc ggcggccgta
     5641 ccggcgcgac agggcagggg cagccgatca ggctctacga cctcaacgaa ggcacgctga
     5701 tcggcctcct cgagggtcac gacgacgccg tgctctcgct tgacttctcg cccgacggcg
     5761 agcgcctggt gtcgggcagc accgacgaca ccgccatcat ctgggacgtg gcgagccgca
     5821 aggcgcttca ccgcctcgtg ggccacaagg gcgatgtgaa cgccgcgcgc ttcaccgccg
     5881 acgcctcggg cgtggtcacc gccagcgacg acaacacgct gaagctctgg gatgcacgcg
     5941 acggggctct cgtctcgacc ctcaccggtc accaggacaa ggtgatcgcc cttgccgtgt
     6001 cgctcaggga cggccaggtc gcctccggcg cgctcgacgg caccatcaag ttctgggact
     6061 tgatcaacgg gcgcgtgctg aaggagatca accagggcac tgaggtgatg agcctcgcct
     6121 ttagcccaga cggcaggcgc ctgctctccg gcgtcggccg cgcgccgtac ctgacccatg
     6181 tctgggacgc cgcaaccgga agcgaagtgg cgagctatgg cggtcacgac aacatcgtgc
     6241 tggccacggc ccttgccgaa aatggcctcg ccgctaccgc cggcggcaac gacaacgaga
     6301 ttcatctgtg ggacgccgag agcggcaagc tcgtacgcgc tcttgttggt gtcggccgcg
     6361 ccatctggtc cgtgggcttt tcgcctgacg gacgctccat cgcctggggc gtcgagcggc
     6421 ctggaagcgg cgagctcggg agcctcgcct tcagcctgaa gcttccggca agagacagtc
     6481 ccctcggcga gcccaaggca cttcgtgccg gtgccgatga attcgccaga gcggtgaccg
     6541 aggcgggcgg cttcagcctg caaacgcggg cgagcggcga gttcggctat ctcgacctgc
     6601 tcgacgtggt gaaggacggc aaggtcctcg ccaccgtcaa gcgcggcgag aatgccggct
     6661 acgtccataa cgcttatacc ttcacgcccg acggcaagac cgtgatcagc ggcggaggcc
     6721 acggctttct caccgcctac gatctctcgg ggcgcaccct cggcgagttc gtcggacaca
     6781 cgagcgacgt ctgggctgta gccgtctccc ccgacgggcg ttggctcctc tccggcagcg
     6841 acgatcagac cgtgcgcctg tggagcatgg cgacgcatga gaatgtggcg tcgctgttct
     6901 acggcaagaa cggcgagtgg gtgttatgga ccccgcaagg ctactacgcg gcctctccgt
     6961 ctggcgacgc tcatgtcggc tggcacgtca atgaaggcga gggcaagctc gcccgctttg
     7021 tcagcgccgc ccagctgaaa agccatttct atcggcccga catcgtcacc cgcgcgctcg
     7081 tgctcggcag cgccgcgcgt gccgtgaaag ag
//
LOCUS       DATNAE010000267         2426 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_870873, whole genome shotgun sequence.
ACCESSION   DATNAE010000267 DATNAE010000000
VERSION     DATNAE010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_870873"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="VN518_05995"
     CDS             <1..486
                     /locus_tag="VN518_05995"
                     /inference="COORDINATES: protein motif:HMM:NF028004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HXE04160.1"
                     /translation="LQRLRLLSATLGLLAVGAFSAEPADDIKPPSGYRQWFHVNTMII
                     DKGSPLFADLGGMHNVYVNSTGLAALKKGGPYPDKSTFVTDLHEFTVSDGSYVEGARK
                     ALAIMVKDAKKYASTGGWGFQLWASGDANKPMVTDASKQCFACHQPKKDQDYVYSTYI
                     P"
     gene            complement(514..1980)
                     /locus_tag="VN518_06000"
     CDS             complement(514..1980)
                     /locus_tag="VN518_06000"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXE04161.1"
                     /translation="MTPLLEVAGVSKRFGGVQAVRGLNLAVHDGEILGLIGPNGAGKS
                     TVFNLINGVYPPDRGRIAFKGEDITGKPPYRIARHGIARAHQIVQPLAGMTVLENCTV
                     GACFGRENLPLARAQDVVREAAEIVGLADRLDQPAGQLTTAGKKRLELARALSARPEL
                     LLLDEVLAGLNPTEIERMIAVIRRIRDRGIAILIIEHVMRAIMRLSDRIVVLNLGGKL
                     AEGTPRSVANNPEVIAAYLGAPEPAVKARSLEMSEPLLRVEALEAGYDEVQVLWGISL
                     GAAPGTTTTLVGANGAGKTTSLRAITGNIQPFGGRVFFAGEDVTQLAPHAKAERGLVL
                     VPEGRQLFDTMSVQDNLEMGAFSKRARRRFAERLDHIYTLFPRLKERARQKAGTLSGG
                     EQQMLAIARGLMSDPEVLIIDELSLGLAPVVVQQLASTLRALKESGMTILLVEQNVQL
                     ALALSDYAYVIAEGRAFAEGSSAEVARKPEIRQAYLGL"
     gene            complement(1977..>2426)
                     /locus_tag="VN518_06005"
     CDS             complement(1977..>2426)
                     /locus_tag="VN518_06005"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXE04162.1"
                     /translation="FLSYAIKSSKFGLGLLAIGENEDAAAVLGVRTPRYKALAYSASA
                     FLPAIVGGLFFFKSGIIDPAGAFDLTGSIEAIVMVMLGGLGTVTGAALGAFLYEELRG
                     VLLTTEAFSHFQLVIAGVLLLAIVLFAPGGLMGFIYRRWPRMRRLLE"
BASE COUNT          454 a          784 c          771 g          417 t
ORIGIN      
        1 ttgcagagac tacgcttgct ttcggcaacc cttggcctcc ttgccgtcgg cgcattctcc
       61 gccgaacccg ccgacgatat caagccgccg agcggttatc ggcagtggtt ccacgtcaat
      121 accatgatca tcgacaaagg aagcccgctg ttcgccgatc tcggcggaat gcacaacgtc
      181 tacgtcaatt cgaccggact agccgcgctc aagaagggcg gaccttatcc cgataagagc
      241 actttcgtga ccgacttgca tgaattcacg gtttccgacg gctcgtacgt ggaaggcgcg
      301 cgcaaggcgc tggccatcat ggtgaaggac gcgaagaagt acgcatcgac cggcggctgg
      361 gggttccagc tctgggctag cggagacgcg aataagccca tggtgacgga tgcctccaag
      421 cagtgcttcg cctgtcatca gccgaagaaa gaccaggact acgtctattc gacttacatt
      481 ccctgacgcg cggagcgagg cagccgacaa ggcttaaagt ccgagatagg cttgccggat
      541 ctcgggtttc cgcgccacct cggcagatga tccttcggcg aacgcccgcc cctcggcgat
      601 gacataggcg tagtcgctca gcgccagtgc cagctgcacg ttctgttcga cgagcaggat
      661 ggtcatgccc gactctttca gggctctcag cgtgctcgcg agttgctgca cgacgacggg
      721 ggccaatccg agcgacaatt cgtcgatgat caagacttcc ggatcggaca tgaggccgcg
      781 cgcgatcgcc agcatctgct gctcaccgcc ggaaagcgtg ccggcctttt gtcgagcgcg
      841 ttctttcagc cgcggaaaca gcgtgtagat gtgatcgagc cgctcggcga agcggcgccg
      901 cgcgcgcttg gagaaggcgc ccatctccaa gttgtcctgc acgctcatcg tgtcgaacaa
      961 ctggcgccct tcgggcacca gcaccaatcc gcgctcggcc ttggcgtgcg gagcgagttg
     1021 ggtgacatct tcgccggcga agaacaccct gccgccgaat ggctggatgt ttccggtaat
     1081 ggcgcgcagg ctcgtcgttt ttccggcgcc attggcgccg accaacgtcg tcgtggtgcc
     1141 tggcgccgcg ccgagcgaaa tcccccagag gacttggacc tcgtcatagc ccgcctcgag
     1201 ggcctctacg cgcaacagcg gctcgctcat ttcgagactc cgcgctttga ccgccggctc
     1261 gggcgcgccg agataagcgg caatgacttc cggattgttc gccaccgagc gcggcgttcc
     1321 ctcggcgagc ttgccgccga gattgagcac cacgatccgg tcggacaacc tcatgatcgc
     1381 gcgcatcaca tgctcgatta tcaggatggc gatgccgcgg tcgcggatgc ggcggatcac
     1441 cgcgatcatt cgctcgatct cagtcggatt gagaccggcg agcacctcgt cgagcagcag
     1501 aagttcgggg cgcgcgctca gagcgcgagc aagctccagc cgcttcttgc cggcggtcgt
     1561 caattgccca gcgggctgat ccaaacgatc ggcgaggccg acgatctcgg ctgcttcgcg
     1621 cacgacgtcc tgcgcacgcg cgagcggcaa gttctcacgc ccgaagcagg cgccgacggt
     1681 gcaattctcg agcaccgtca tgcccgcgag cggctgcacg atctggtgcg ctcgggcgat
     1741 accgtggcgg gcgatgcggt aaggcggctt gccggtgatg tcctctccct tgaatgcgat
     1801 gcggccgcga tcgggcggat agacgccgtt gatgagattg aacacggtcg acttgccggc
     1861 gccgttgggg ccgatgaggc caaggatctc gccgtcgtgc acggcgaggt tcaggccgcg
     1921 cacggcctgc acgccgccaa agcgcttgct gacaccggca acttcgagca ggggcgtcat
     1981 tcgagcagcc tccgcattcg cggccagcgc cggtagatga aacccatgag cccgcccggg
     2041 gcgaacagca cgatggcgag tagcaagact cccgcgatca cgagctggaa gtgcgagaac
     2101 gcctcggtcg tcagcagcac gccgcgcagc tcttcgtaga ggaaagcccc gagcgccgcg
     2161 ccggtcaccg tgcccagccc gccgagcatg accatgacga tcgcctcgat cgatcccgtc
     2221 agatcgaacg ctccagccgg gtcgatgatg ccgctcttga aaaagaacag gccaccgacg
     2281 atcgccggca ggaaagcaga cgcgctgtag gcaagcgcct tgtaacgcgg cgtgcgcacg
     2341 ccgagaacgg cggcggcgtc ctcgttctcg ccgatggcga gaagcccgag gccgaacttg
     2401 gagcttttga tggcgtagct caggaa
//
LOCUS       DATNAE010000268         2389 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_270782, whole genome shotgun sequence.
ACCESSION   DATNAE010000268 DATNAE010000000
VERSION     DATNAE010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270782"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="VN518_06010"
     CDS             <1..386
                     /locus_tag="VN518_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HXE04163.1"
                     /translation="SRGATMDLTLVPLDTDPVRPATGGNALGACTAPAAMREADTSID
                     MGTGFDCFDVKANTAVPGLSMAQQQNRQMLVDAMARHGFKSYDKEWWHFTLENEPFPN
                     TVFDFPIDPRDTRPIDGSTSPPAPK"
     gene            426..1871
                     /locus_tag="VN518_06015"
     CDS             426..1871
                     /locus_tag="VN518_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXE04164.1"
                     /translation="MPRTSTEPSFITTDIPARLERLRWGHFHSLVVIALGITWALDGL
                     EVTLAGAVAGALKDSASLALSNAQVGLAASFYLAGAVLGALFFGWLTDRLGRKRLFSI
                     TLGLYLIATAATALSWDFYSFSLFRFLTGAGIGGEYTAIASAIQEFIPARYRGWTDLV
                     INGSFWIGAAIGAASSVVLLDPMVLAPDFGWRAAFFIGAFLGLSILLLRAWIPESPRW
                     LVTHGRLKEAQAVLADIEDRHRRHSGPLPPLEHPPVKLRPRRFTSLAEVWHTLYRKHR
                     RRTFVGLTLMASQAFFYNAIFFTYALVLTDFYGIPADHVGWYLLPFTIGNFLGPVMLG
                     RLFDTVGRRPMIALTYALSGVLLALTGYLFAIGALSATSQTIAWTIIFFFASAAASSA
                     YLTVSETFPLEVRALAIAFFYAVGTGVGGVVGPGLFGVLIETGSRTSLALGYGLGAAF
                     MLAAAVVAGCYGVAAERKPLEAVARPLSAVD"
     gene            complement(1900..2322)
                     /gene="dksA"
                     /locus_tag="VN518_06020"
     CDS             complement(1900..2322)
                     /gene="dksA"
                     /locus_tag="VN518_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HXE04165.1"
                     /translation="MRPKRKIPEDYRPSDDEPFMNERQRAYFRRKLVAWRDEILRSTK
                     ETLQHLQDESAQHADIADRASTETERALELRARDRQRKLIAKIDAALARLDEGTYGYC
                     EETGEPISLKRLDARPIATLSVEAQERHERRERVYRDE"
BASE COUNT          358 a          807 c          719 g          505 t
ORIGIN      
        1 actcgcgcgg cgccaccatg gatctcacgc tggttccgct cgacaccgat ccggttcggc
       61 ccgcgaccgg cgggaatgct cttggcgcct gcacggcgcc ggcggcgatg cgtgaagccg
      121 acaccagcat tgacatgggc accggcttcg actgcttcga tgtgaaggca aacacggcag
      181 tccccggcct cagcatggcg cagcagcaaa accgccagat gctggtcgac gccatggcgc
      241 gccacggctt caagagctac gacaaggaat ggtggcactt tacgctcgag aacgagccgt
      301 tccccaatac cgtgttcgac tttccgatcg acccgcgcga cacacggccg atcgatgggt
      361 caacgtcgcc gcctgcacca aaatagcgct gtcctcccac gccggcatca agccaaggag
      421 gcgtcatgcc gcgaacctcg accgagccat cgtttatcac caccgatatc ccggcgaggc
      481 tcgaacggct ccgctggggg cacttccact cattggtggt gatcgcgctc ggcatcacct
      541 gggctctcga tgggctcgag gtgaccctcg cgggcgcagt ggcgggcgca ctcaaagaca
      601 gcgctagcct tgcgctctca aatgcgcaag ttgggctggc ggccagtttc tatctcgctg
      661 gtgcggtgct cggcgccctc ttctttggct ggctcaccga ccggctaggg cgcaagcgcc
      721 tgttctccat caccctaggt ctctacctga tcgccacggc ggcaacggcg ctgtcgtggg
      781 acttctacag tttctcgctc tttcgcttcc tgaccggggc cggcatcggc ggcgaataca
      841 cggcgattgc ctcggccatc caggagttca tcccggcccg ctatcgcgga tggaccgacc
      901 tcgtcatcaa tggcagtttc tggatcggag ccgccatcgg cgcggcaagc tccgtggtcc
      961 tcctcgaccc tatggtcctg gcaccagact tcggctggcg cgccgccttc ttcatcggcg
     1021 cctttctcgg gctctctatt ctgctcctgc gcgcatggat ccctgagtcg ccgcgttggc
     1081 tcgttaccca cggcagattg aaagaggcgc aagccgttct cgccgatatc gaagaccgcc
     1141 atcggcggca ctctgggccc cttcccccgc tcgagcatcc tcccgtcaag ctcaggccac
     1201 gtcgcttcac ttcgctcgct gaggtgtggc acaccctcta tcgcaagcat cgtcgccgaa
     1261 cctttgtcgg gctcaccctg atggcctcgc aggcgttctt ctacaatgcg atcttcttca
     1321 cctacgcctt ggtgctcaca gacttctacg gcattcccgc cgatcacgtt ggctggtatc
     1381 tgcttccctt caccattggc aattttcttg ggccggtgat gcttggcagg ttgttcgata
     1441 cggtcggccg caggccgatg atcgcgttga cctacgcgct ctccggggtc ctgctcgccc
     1501 tcaccggcta tcttttcgcg atcggcgcgc tttcggccac gagccagacc atcgcctgga
     1561 caatcatctt cttctttgcc tcagcggccg cgagctccgc ctatctcacc gtgagcgaaa
     1621 catttccgct cgaggttcgc gcgctcgcca tcgcgttctt ctatgctgtt ggtaccggcg
     1681 taggcggcgt cgtcggaccg ggcttgttcg gcgtgctcat cgagacgggc tcgcgtacca
     1741 gcctcgctct tggttatggg ctcggggcag ccttcatgct tgccgcggcg gtcgttgccg
     1801 ggtgctacgg cgtggcggcc gaacgcaagc cgctcgaagc cgtggcgcgg cctttgtcgg
     1861 ctgtagacta ggatgatgaa agcttggctt aagcggcagc tattcgtcgc ggtagacgcg
     1921 ctcgcgccgc tcgtggcgct cctgggcttc gacgctcaag gtcgcgatcg gacgtgcatc
     1981 gaggcgcttc aggctgatcg gctcgccggt ctcctcgcaa tagccgtagg tgccctcatc
     2041 gagacgggca agggcggcgt cgatcttggc aatcagcttg cgctggcggt cacgggcgcg
     2101 aagctcgagc gcccgctcgg tctccgtgct tgcccggtcg gcgatatcgg cgtgctgagc
     2161 cgattcatcc tgcaggtgct ggagcgtctc tttggtcgag cggaggatct cgtcgcgcca
     2221 agcgaccagc ttgcgccgga aataggcccg ctggcgctcg ttcatgaaag gctcatcgtc
     2281 ggagggccga tagtcttcgg ggattttacg cttcggccgc atgaaagcct ctgccaaccg
     2341 catggtcttt attcctcggg ccaaaggccc cgtcgtgtcc ccagccggt
//
LOCUS       DATNAE010000269         2407 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2375320, whole genome shotgun sequence.
ACCESSION   DATNAE010000269 DATNAE010000000
VERSION     DATNAE010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2375320"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2358
                     /locus_tag="VN518_06025"
     CDS             <1..2358
                     /locus_tag="VN518_06025"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HXE04166.1"
                     /translation="PNAPGADEVAASTSTVNRTSDQAAHVSIVPLPDRNPTAGAPVLA
                     AAEEISLLPHSASLVPPPPVPQAAPAVPTPAPSAPAGAADDTPLPDPNPNRKATLPIV
                     MKGTLTPAIQAPAPALDYAKILKPLTSYELSDGDESALRDVLHDGGTAAASGIKDEAA
                     RAFALWFRYRSTGVNGSAEAIEQFRLAHPDWPAQDDLREKAETALFLNDASPEAVKAF
                     FASSSPQTGAGKAALAGVYLKEGNEQGARDLVTSAWRDHQLNQAVETKILAKYGYMLT
                     AETHRARIDRLLYPDDKSSIAAALRVAKLLPADEQKKIAARIAVVQRGGNAGKLLEAL
                     PANAVEADAGLRFNRIQWLRRKDRDKEAWKMLLDAPSEPNVLLDLNNWWVERRVNCRE
                     ALNAGQARIAYEIAEKHGLVSGDAYIEAEFLAGWIALRFLADPDTALRHFVALRSAAT
                     SSKSIALGEYWLGRTSLALGDRGSAIVHFHAAAKYPQYFYGQLGRQALDPKPARLEVT
                     STPLPTDADIQRFLSRDTVRAIGIAHDVGLDGVTGQFFLALSRKLDSPQEVVLLAELA
                     KQMGQPQLALRLAKIAFNRDLPVGDYALPIGVIPEFRSLLTERVDPALVHALSRQESE
                     FNAAAKSPVGASGLMQLMPSTARAIANAYKVKFDANQLTNAAYNTQLGEAHLKQLIDS
                     YNGSYFLSLAAYNAGGGRVADWIKQFGDPRDPKVDPVDWIERIPFTETRQYVQKIMET
                     LQLYRSRLAGPTQALQLVQDLNRGKRIPRDAAASAEASVVQAKSE"
BASE COUNT          444 a          845 c          710 g          408 t
ORIGIN      
        1 cccaatgcgc ctggagcgga cgaggtggcc gcctcgacat ccacggtcaa ccgcacatcg
       61 gatcaggccg cgcacgtgtc gatcgtcccc ctgcccgacc gtaaccccac ggccggcgca
      121 cccgtgcttg cggcggctga ggaaatctcc ttgctcccac attcggcctc gttggtcccg
      181 ccaccgcccg tgcctcaagc ggcgccggcc gtaccgactc ccgctccatc cgcacctgct
      241 ggggcagccg acgacacgcc actgccggat cccaatccca accgcaaggc gacgcttccc
      301 atcgtcatga agggcacgct cacgccggcc atccaggcgc cggctccggc cttggactat
      361 gcgaaaatcc tgaagccctt gacctcttat gagctcagcg acggcgacga gtctgccctt
      421 agggacgttt tgcatgacgg cggcaccgcg gcagcgtcgg ggatcaagga cgaagcggcg
      481 cgcgcgttcg cgctctggtt tcgctatcgg tcgaccggcg tcaacgggag tgccgaggcg
      541 atcgagcagt tccgcctcgc ccaccccgat tggccggctc aggacgatct gcgcgaaaag
      601 gccgagaccg ccctcttcct caacgatgcc agtcccgagg cggtcaaggc gttcttcgcc
      661 tcttcgagtc cacagacggg cgccggcaag gcggcgcttg ccggtgtcta tctgaaagaa
      721 ggcaacgagc aaggcgcccg cgacctcgtc acctcggcgt ggcgcgacca ccagctcaac
      781 caggccgtcg agaccaagat tctcgccaag tacggctaca tgctcaccgc cgagactcat
      841 cgtgcgcgca tcgaccggct gctctatccg gacgacaaat catccatcgc cgcagcgctt
      901 cgcgtcgcga agctccttcc cgccgatgag cagaagaaaa tcgccgcgcg aattgccgtg
      961 gttcagaggg gtggaaacgc cggcaagttg cttgaagctc tgcccgcgaa cgcggtcgag
     1021 gccgacgccg gcctccggtt caaccgcatc cagtggctcc gccgcaagga ccgcgacaaa
     1081 gaagcgtgga agatgctgct ggacgctccg agcgagccta acgttctgct cgatctcaac
     1141 aattggtggg tcgagcgccg tgtcaattgc cgcgaggcgc tgaatgccgg tcaggctcgc
     1201 atcgcatacg agatcgccga gaagcacgga ctcgtctcgg gcgatgctta tatcgaggcc
     1261 gaattcctcg ccggctggat cgcgctcaga ttcctcgccg accctgacac cgccttgcgc
     1321 cacttcgtgg cgctccgaag cgcggcgaca agctccaaga gcatagcgct cggcgaatat
     1381 tggctcgggc gtacctcgct tgcgctgggc gaccgcggct cggcgatcgt ccacttccac
     1441 gcagcagcca aatacccgca atatttctac gggcagctcg gacgccaagc gctcgatccg
     1501 aagcccgccc gtctcgaggt cacgtcgacc ccgctaccca ccgatgccga cattcagcgc
     1561 ttcctgtcgc gcgataccgt gcgcgccata gggattgcgc acgacgtcgg ccttgatggg
     1621 gtgacggggc aattcttcct cgctctgtcg cgcaagctcg atagcccgca agaggtggtg
     1681 cttctggccg agcttgccaa gcaaatgggg caaccgcagc tcgcgctcag gcttgccaag
     1741 atcgccttca accgcgatct gcctgtcggt gattatgccc tgcccattgg cgtcatcccg
     1801 gaattccgca gcctcctcac cgagcgcgtc gatcctgcac tcgtgcatgc gctgtcgcgg
     1861 caggagagcg aattcaatgc cgccgccaag agccccgtcg gcgcaagcgg cctgatgcag
     1921 ctgatgccga gcacggcgag agccatcgcc aatgcctaca aggtgaagtt cgatgcgaac
     1981 cagctcacca atgccgccta caatactcag ctcggcgagg cgcatctcaa gcaattgatc
     2041 gacagctata acgggtcgta tttcctctcg ctcgccgcct ataacgccgg cggcggccgt
     2101 gtcgccgact ggataaagca gttcggcgac ccccgcgatc cgaaagtcga tccggtagac
     2161 tggatcgagc gcatcccctt caccgagacg cggcaatatg tgcagaagat catggagacc
     2221 ctgcagctct atcgctcacg cctggcaggg cctacgcaag cgctccagct cgtccaggac
     2281 ctcaatcgcg gcaagcggat cccgcgtgac gccgctgctt cggccgaggc ttcggtggtg
     2341 caagccaagt ccgagtgaag gcgccccgcc cgcatcattc ttcggggctt gccccctcgt
     2401 gaacttt
//
LOCUS       DATNAE010000270         3485 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2079701, whole genome shotgun sequence.
ACCESSION   DATNAE010000270 DATNAE010000000
VERSION     DATNAE010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2079701"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(463..861)
                     /locus_tag="VN518_06030"
     CDS             complement(463..861)
                     /locus_tag="VN518_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HXE04167.1"
                     /translation="MKKSVAQDSRSATRLIDARIKELGDWRGEMLARLRAVIKQADPE
                     VVETWKWRGVPVWEHDGIICTGETYKTVVKLTFLKGASLEDPSRLFNASLEGNARRAI
                     DIHEGAKIDEKALKALIRAAVSLNTSKARG"
     gene            complement(871..1317)
                     /locus_tag="VN518_06035"
     CDS             complement(871..1317)
                     /locus_tag="VN518_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018098334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HXE04168.1"
                     /translation="MTKLASDTRSVIVERELPHAPHKVWRALTESPLIEDWLMKNDFQ
                     PRRGHKFTFRAEPQPYWNGVTDCEVLEIEPPTRLSYSWNASGAEAATGVKTVVTWTLT
                     PTGSGTLLRMEQSGFRPDQDNNYQGAHYGWPKFIAGLERTVAGLGD"
     gene            complement(1314..1637)
                     /locus_tag="VN518_06040"
     CDS             complement(1314..1637)
                     /locus_tag="VN518_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015316758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HXE04169.1"
                     /translation="MHAAPDLIFRTLADPTRRAIFERLAQGGEQTVHRLTDLSGVSQP
                     AVSKHLGLLKQAGLVRDRRAGRETHYSAQPEGLAPLIDWMGHYGAFWRDRFDRLEDLL
                     NRMDQ"
     gene            1686..1928
                     /locus_tag="VN518_06045"
     CDS             1686..1928
                     /locus_tag="VN518_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018329290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HXE04170.1"
                     /translation="MGSSSRTWYGIAAKADWIGPADVKEQFGPNVDFLKDNRLVFDLG
                     GNKYRLVVRVSYTFKAVLVKFIGTHRAYDRIDAETV"
     gene            complement(2120..2440)
                     /locus_tag="VN518_06050"
     CDS             complement(2120..2440)
                     /locus_tag="VN518_06050"
                     /inference="COORDINATES: protein motif:HMM:NF024979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HXE04171.1"
                     /translation="MITLDELCASLGARQTEVRGWIQAGFVEPIGESQSWRFADVDAA
                     RVRLIFELRYQMNVVEEDLDLVLRLLDQVYGLRRELQALAQAVSRQPDAVRSAIAAAM
                     KEGG"
     gene            complement(2437..3375)
                     /locus_tag="VN518_06055"
     CDS             complement(2437..3375)
                     /locus_tag="VN518_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006023628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HXE04172.1"
                     /translation="MAADPYSVLGVGKEASEKEISSAYRKLAKQFHPDLNPGNKDAES
                     RFKQIAAAHDLLSDKEKRAKFDRGEIDAEGQPVPERGWYRPHAEGADGAHYASSGGYE
                     DLGDMFSDLFRERAAAGGRGAGGRGRRAQMQLPGEDVSYNLPVDFLEAARGAKKRVTM
                     PDGKVLDITIPEGIKDRQALRLKSQGQPGYNGGPPGDAYVELHIQPHAFFERKDEDVH
                     LELPITLGEAVLGGKIEVPTVHGPVSMTVPKGANSGTTLRLKGRGIKTGGTAGDQYVK
                     LKLVLPEQPDPELESFVRDWAPKHAHDPRAAMRSFA"
BASE COUNT          548 a         1131 c         1167 g          639 t
ORIGIN      
        1 cacttgttgc gagcatccac gccttgctct gaagcgatca ccgctcgccc gcaaaggcgt
       61 ggaccctcgc gacaagcgcg agggcgacgg cctgagaggc ggaagcggcg gagcaaaggc
      121 gtgggccctc gcgaccagcg cgagggatga caggtgcaga tggaaaactg cctcctccac
      181 tccgccgtca tgctcgcact cgttgcgagc atccacgcct tgctctgaag cgatcaccgc
      241 tcgcccgcaa aggcgtggac cctcgcgaca agcgcgaggg tgacggcctg agaggcggaa
      301 gcggcggagc aaaggcgtgg gccctcgcga ccagcgcgag ggatgacagg tgcagatgga
      361 aaactgcctc ctccactccg tcgtcatgct cgcactcgtt gcgagcatcc acgcctttcc
      421 tcgaagcgac catcggcggc gggaggagtc ggagatctcc gatcacccgc gcgctttcga
      481 tgtattcagg ctgacggcgg cgcgaatgag cgccttcagc gccttctcgt cgatcttcgc
      541 gccctcatgg atgtcgatgg cgcgccgcgc gttgccctcg aggctggcgt tgaacaggcg
      601 cgacggatcc tcgagcgagg cgcctttgag gaaggtcagc ttcaccaccg tcttgtaggt
      661 ctcgccggtg cagatgatgc cgtcatgctc ccagaccggc acgccgcgcc atttccaggt
      721 ctcgaccacc tcgggatcgg cttgcttgat cacggcccgc aagcgggcga gcatctcgcc
      781 gcgccaatcg ccgagctcct tgatgcgcgc gtcgatcagc cgggtggcgg accggctgtc
      841 ctgcgcgacg ctcttcttca tctcccctcc tcagtcgccg agcccggcga cggtgcgctc
      901 gaggccggcg atgaatttcg gccagccata gtgcgcgccc tgataattgt tgtcctggtc
      961 gggacggaag cccgactgct ccatgcgcag cagcgtgccc gagccggtcg gcgtcaaggt
     1021 ccaggtcacc acggtcttca ccccggtcgc cgcctcggcg cccgaggcgt tccagctgta
     1081 ggagagccgc gtcggcggct cgatctcgag cacctcgcaa tcggtgacgc cgttccaata
     1141 gggctgcggc tcggcgcgga aggtgaattt gtgtccgcgc cggggctgga aatcgttctt
     1201 catcagccag tcttcgatga gagggctttc ggtcagcgcc cgccacacct tgtgcggggc
     1261 atgcggcagc tctcgttcga cgatcacgga gcgcgtatcg gaggcgagct tggtcattga
     1321 tccatcctgt tcagcaggtc ttcgaggcga tcgaaccggt cgcgccagaa ggcgccgtaa
     1381 tgacccatcc agtcgatcag gggggcgaga ccttcgggtt gggcgctgta atgggtctcg
     1441 cggccggcgc gccggtcgcg caccaggccg gcctgcttga gcaggccgag atgcttcgac
     1501 accgccggct gcgacacgcc ggagaggtcg gtgagccggt gcaccgtctg ctcgccgcct
     1561 tgggcgagcc gctcgaagat cgcccgccgc gtcggatcgg cgagggtcct gaagatcagg
     1621 tcgggtgcgg cgtgcatggc aaaccataac cagtgggtta tcgattattc aatagccgac
     1681 tgattatggg ttcgtcaagc cgcacctggt acgggatcgc ggccaaggcc gattggatcg
     1741 gtcccgcgga tgtgaaggaa cagttcggcc ccaatgtgga ttttctcaaa gacaaccggc
     1801 tggtctttga tctcggcgga aacaaatatc ggctcgtggt tcgggtcagc tacacattca
     1861 aggcggtgct ggtgaagttc atcggtacgc atcgcgcgta tgacaggatc gacgcggaaa
     1921 ccgtgtaatg gaaatcaatc cgatcaagcg cgagggtgac gacggaggag gtggtgcggc
     1981 atgagccctt cactctagct cgtcatgctc gcactcgttg cgagcatcca cgccttcgct
     2041 ggcggctatc gtcgcttcag agaaacacct ggcccctcag tgagcgcgag ggtgacgggg
     2101 gaattgaaag ctccgtgctc taaccgccct ccttcatcgc cgccgcgatg gcgctgcgca
     2161 ctgcatcggg ctggcggctg accgcctgcg ccaacgcctg cagctcgcgc cgcaggccat
     2221 agacctggtc gagcagccgc aagaccaggt cgagatcctc ctcgacgaca ttcatctggt
     2281 agcgcagctc gaagatgagc cgcacgcgcg ccgcatcgac gtcggcgaag cgccaggatt
     2341 gggattcgcc gatgggttcg acgaagccgg cctggatcca gccgcgcacc tcggtctgcc
     2401 gggcgccgag gctggcgcag agttcgtcga gcgtgatcat gcgaagctcc tcatggcggc
     2461 gcgcggatcg tgcgcatgct tcggcgccca gtcgcgcacg aagctctcga gctcgggatc
     2521 gggctgctcg ggcagcacga gcttgagctt cacatattgg tcgcccgcgg tgccgccggt
     2581 tttgatgccg cggcccttga ggcgcaaggt ggtgccgctg ttggcgccct tgggcaccgt
     2641 catgctgacc gggccatgca ccgtcggcac ctcgatcttg ccgccgagca cggcctcgcc
     2701 gagcgtgatc ggcagctcga gatgcacgtc ctcgtccttg cgctcgaaaa aggcgtgcgg
     2761 ctggatatgg agctcgacat aggcgtcgcc gggcgggccg ccattatagc cgggctggcc
     2821 ctggcttttg aggcgcagcg cctggcggtc cttgatgccc tcggggatgg tgatgtcgag
     2881 caccttgccg tcgggcatgg tgacgcgctt cttggcgccc cgtgccgcct cgaggaaatc
     2941 gaccggcagg ttgtaggaga cgtcctcgcc gggaagctgc atctgcgcgc gccggccgcg
     3001 cccgccggcg ccccgtccgc ccgccgcggc gcgctcgcga aacagatcgg agaacatgtc
     3061 gccgagatcc tcatagccgc cgctcgaggc gtaatgcgcc ccgtcggcgc cttcggcatg
     3121 cggccggtac cagccgcgct cgggcaccgg ctggccctcg gcatcgatct cgccgcggtc
     3181 gaacttcgcc cgcttctcct tgtccgacag gaggtcgtgg gcggcggcga tctgcttgaa
     3241 gcggctctcg gcgtccttgt tgcccggatt gaggtcggga tggaactgct tggcgagctt
     3301 gcggtaggcc gagcttatct ccttttcgct cgcctccttg ccgacgccga gcacgctata
     3361 gggatctgcc gccatgtccg gtcgctttcc gagtggttct agggtctgtc ctcgtatccc
     3421 tcgcggagct gcgccggtcg attttggccg tggatcaagg ctggtgccga cgcgcgatgc
     3481 tgtcg
//
LOCUS       DATNAE010000271         4100 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_571771, whole genome shotgun sequence.
ACCESSION   DATNAE010000271 DATNAE010000000
VERSION     DATNAE010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4100
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571771"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(190..945)
                     /locus_tag="VN518_06060"
     CDS             complement(190..945)
                     /locus_tag="VN518_06060"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HXE04173.1"
                     /translation="MREDRFRKAAKERRTLFNAWLTLNNPFLIELIGEAGWDCLTIDQ
                     QHGLGGNEALLGCLIAARACGLPAIVRVAENDPGLIGRALDAGAQGIMCPLIGSVEDA
                     EAFVQAVKFPPRGNRSWGPYRARLDHEGDYFKTANAWTIACPQIETKGALDQLDEILA
                     LDGVDMVCLGPNDLSVALTGRLDIWAPEVKDAMTLVLSKCREMSVMALIFANDADYAK
                     PRVAEGWDVVAVGTDAGWFSKAAAEIKAAASNS"
     gene            complement(956..1285)
                     /locus_tag="VN518_06065"
     CDS             complement(956..1285)
                     /locus_tag="VN518_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008193764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1476 domain-containing protein"
                     /protein_id="HXE04174.1"
                     /translation="MTSFEDREKGFERKFAHDEELKFKATARRNRLLGLWAAEQMGVT
                     GEAAQAYAREVIKADLVAPGEEDLFGKIRSDFDAKGVSQSDHQIRRQMAELMGVAIGQ
                     IESEAKG"
     gene            1588..2358
                     /gene="purC"
                     /locus_tag="VN518_06070"
     CDS             1588..2358
                     /gene="purC"
                     /locus_tag="VN518_06070"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007009773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HXE04175.1"
                     /translation="MNRRRRVYEGKAKILYEGPEPGTLVQHFKDDATAFNNKKHDKIE
                     GKGVLNNRISEYLFMKLGEVGVPTHFIKRLNMREQLIREVEIIPLETVVRNVAAGSLS
                     TRLGIDEGTALPRSIVEFYYKSDALGDPMVTEEHITAFGWATPQEIDDVMSLALRIND
                     FLSGLFLGVGIKLVDFKCEFGRLWENEQMRIVLADEISPDCCRLWDIETQNKLDKDRF
                     RRDMGGLVEAYQEVARRLGLHVDNPTRTRTGPVLVRSK"
     gene            2377..2622
                     /gene="purS"
                     /locus_tag="VN518_06075"
     CDS             2377..2622
                     /gene="purS"
                     /locus_tag="VN518_06075"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018701205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HXE04176.1"
                     /translation="MKARITITLKQGVLDPQGKAIAGALHALGFGSVGNVRQGKYIEV
                     EVVENDPAKARAEVERMCEQLLANTIIENYAYELEAA"
     gene            2742..3431
                     /gene="purQ"
                     /locus_tag="VN518_06080"
     CDS             2742..3431
                     /gene="purQ"
                     /locus_tag="VN518_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HXE04177.1"
                     /translation="MKASVIVFPGSNCDRDAAVALEAATGEAPAMVWHGDSVLPASDL
                     IVLPGGFSYGDYLRSGAMAAHSPVMREVVARARAGTPVLGICNGFQVLTEAGLLPGVL
                     MRNATLKFICRDVTLRVERNDSIYTRRYQRGEVVRFPIAHKDGSYFADGATLQRLEGE
                     GRVAFRYCDAGGNITPEANPNGSRNNIAGIYNETGTVLGLMPHPERLADVALSGTDGA
                     KLFLSLVETLH"
     gene            3428..>4100
                     /locus_tag="VN518_06085"
     CDS             3428..>4100
                     /locus_tag="VN518_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003495359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HXE04178.1"
                     /translation="MNIAARAERAADGITPELVAEHGLSPDEYKLLLTALGREPRLTE
                     LGIFSVMWSEHCSYKSSRVWLRKLPTEGPQVIQGPGENAGVVDIGDGQAAIFKMESHN
                     HPSYIEPYQGAATGVGGIMRDVFTMGARPIANMNALRFGAPDHSKTRHLVACVVSGIG
                     DYGNCMGVPTVGGETNFDPRYNDNILVNAMTVGIADANRIFKSAASGVGNAVVYVGSK
                     TGRDGI"
BASE COUNT          756 a         1354 c         1272 g          718 t
ORIGIN      
        1 tagggataag gcggatagac cggaatgagg cctctattgt ccacgtagcc caacccacga
       61 gggccggcct cggccgcgcc ggcgaaaagc accagcgcga acagaatcat caagcctcga
      121 atttgcatcc gcataggaat cgcccccgtt accccgctcc atcttgccac agagtgggtc
      181 tggcgtcagc tatgagttgc tggccgctgc tttgatttcg gctgcggctt tcgagaacca
      241 gcccgcgtcg gtgccgaccg ccaccacgtc ccagccttct gccacgcgcg gcttggcata
      301 gtcggcgtcg ttggcgaaaa tcagagccat cacagacatc tcgcggcatt tgctgagaac
      361 cagagtcatg gcgtctttga cttccggcgc ccagatatcg agccgcccgg tcagcgccac
      421 cgacaggtcg tttggcccca agcagaccat gtcgacgccg tcgagcgcga gaatctcgtc
      481 gagctgatcg agcgcgcctt tggtctcgat ctgcgggcag gcgatggtcc aggcattggc
      541 ggtcttgaaa taatcgccct cgtggtcgag gcgcgcccgg taagggcccc agctgcgatt
      601 tccccgcggg ggaaatttca ccgcctgcac gaaggcttcg gcgtcctcga cgctgccgat
      661 cagcggacac atgatgcctt gcgcgcccgc gtcgagtgcg cggccgatca ggcccggatc
      721 gttctccgcc acccgcacga tcgccggcag gccgcaggcg cgcgcggcga tgaggcagcc
      781 gagcaaagcc tcgttgccgc ccaacccgtg ctgctggtcg atggtgaggc agtcccagcc
      841 cgcctccccg atcaattcga tgaggaaggg gttgttcagc gtgagccagg cgttgaacag
      901 cgtgcggcgc tctttcgccg ccttgcggaa ccggtcttca cgcatgaagc cgtccttacc
      961 ctttggcttc ggattcgatt tggccgatcg ccacgcccat cagctcggcc atttgacgcc
     1021 tgatctgatg gtccgactgg ctgacgccct tggcgtcgaa atcggacctg atcttgccga
     1081 acaggtcctc ctcgccgggc gccaccagat cggccttgat cacctcgcgg gcataggcct
     1141 gggcggcctc gccggtgact cccatctgct cggccgccca gagcccgagc agccggttcc
     1201 gccgcgccgt cgccttgaat ttaagctcct cgtcatgggc gaatttccgc tcgaaaccct
     1261 tctcgcgatc ctcgaaactc gtcatttggc gtgtcgcctc ctcgggcccc tctcgatgcg
     1321 gggatgctgg ctcggaattc tggggaaact cagtcccgct ggcgccgagg gatagcgctg
     1381 aaatggccgg aaatcaacag attgcggggc caacccgcct gcgtcccacc aatcattgta
     1441 cgcccactcc ccataggcta aggtcgctgc aagacccctt gaatcgattc cggcaatggc
     1501 aatctgtaca tggcgcgcgg cctcattgag cctctcgccc atgcggtttg tcggcagtca
     1561 agccagttgt gagtgaagga accaaacatg aaccgccgcc ggcgcgtcta cgaaggcaaa
     1621 gccaagatcc tctatgaggg ccccgagccg ggcacgctcg tccagcattt caaggacgac
     1681 gccaccgcct tcaacaacaa gaagcacgac aagatcgagg gcaagggcgt cctcaacaac
     1741 cgcatctccg agtatctgtt catgaagctc ggcgaggtcg gcgtgccgac gcatttcatc
     1801 aagcggctca acatgcgtga gcaattgatc cgcgaggtcg agatcatccc gctcgaaacc
     1861 gtggtgcgca acgtcgccgc gggttcgcta tcgacccggc tcggcatcga cgaaggcacc
     1921 gcgctgcccc gctcgatcgt cgagttctat tacaagagcg acgcgctcgg cgacccgatg
     1981 gtcaccgagg agcacatcac cgcgttcggc tgggcgacgc cgcaggagat cgacgacgtg
     2041 atgtcgctcg cgctgcgcat caacgatttt ctctccggcc tgtttctcgg cgtcggcatc
     2101 aagctggtcg acttcaaatg cgagttcggg cggctgtggg agaacgagca gatgcgcatc
     2161 gtgctggccg acgagatctc gcccgattgc tgccggctgt gggacatcga gacgcagaac
     2221 aagctcgaca aggaccgctt ccgccgcgac atgggcgggc tggtcgaggc ctatcaggag
     2281 gtcgcgcggc ggctcggcct gcatgtggat aatccgacgc ggacgcggac cggaccggtg
     2341 ctggtgcgct cgaaatagga tcacacgaag ggggacatga aggcgcgcat caccatcacg
     2401 ctcaagcagg gcgtgctcga cccgcaaggc aaggccattg ccggcgccct gcacgctctc
     2461 ggcttcggct cggttggcaa tgtacgccag ggcaaatata tcgaggtcga ggtagttgag
     2521 aacgacccgg ccaaggcgcg cgccgaggtg gagcgcatgt gcgagcagct tctcgccaac
     2581 accatcatcg agaattacgc ctatgagctc gaggcggctt agcctaaggc gtcgaccatt
     2641 ctcgtcattc cggcgaaagc cggaatccag aaacccgctc acgagctcat ttttcactgg
     2701 atcccggctt tcgccgggat gacgcaccgg attggagcag catgaaagcg agcgtcatcg
     2761 tctttcccgg atcgaattgc gaccgcgacg cggcggtggc gctcgaagct gctacgggcg
     2821 aggcgccggc catggtctgg cacggcgaca gcgtgctccc ggcaagcgat ctgatcgtgc
     2881 tgccaggcgg cttctcctat ggcgactatc tccgctccgg cgccatggcg gcgcattcgc
     2941 cggtgatgcg cgaggtggtg gcacgcgccc gcgccggaac cccggtgctc ggcatctgca
     3001 acggcttcca ggtgctcacc gaggccggcc tgctgcctgg cgtgctgatg cgcaatgcca
     3061 cgctcaaatt catctgccgc gacgtgacac tgcgcgtcga gcggaacgac tcgatctaca
     3121 cgcgccgcta ccaacgcggc gaggtcgtca gattccccat cgcgcacaag gacggcagtt
     3181 atttcgccga cggcgccacg cttcagcggc tcgaaggcga aggccgtgtc gccttccgct
     3241 attgcgacgc gggcggcaac atcacgcccg aggccaatcc caacggctcg cgcaacaata
     3301 tcgccggcat ctataacgag acgggtaccg tgctcgggct gatgccgcat cccgagcgcc
     3361 tcgccgatgt ggcgctttcc ggtaccgacg gcgccaagct gttcctctcg ctggtggaga
     3421 cgctgcattg aacatcgcgg cgcgcgcgga gagagcggct gacggcatca cgcccgagct
     3481 cgtggccgag cacgggctca gtccggacga atacaagctg ctgctcaccg cactcggccg
     3541 cgagccgagg ctgaccgagc tcggcatctt ctcggtgatg tggtccgagc actgctccta
     3601 taaatcctcg agagtgtggc tgaggaagct gccgaccgaa ggcccgcagg tgatccaggg
     3661 tccgggcgag aatgccggcg tggtcgatat cggcgacgga caagcggcga tcttcaagat
     3721 ggagagccac aaccacccgt cctatatcga gccctatcag ggcgcggcca ccggcgtcgg
     3781 cggcatcatg cgcgacgtgt tcaccatggg ggcgcgcccg atcgccaaca tgaacgcgct
     3841 cagattcggc gcgcccgatc actccaagac gcgccacctc gtggcctgcg tcgtgtcggg
     3901 catcggcgac tacggcaatt gcatgggcgt gccgaccgtc ggcggagaga ccaatttcga
     3961 tccgcgctat aacgacaaca tccttgtcaa cgccatgacc gtcggcatcg ccgacgccaa
     4021 ccgcatcttc aagtccgcgg cgagcggcgt cggcaatgcc gtggtctatg tcggctcgaa
     4081 gaccggccgc gacggcatcc
//
LOCUS       DATNAE010000272         8448 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3877187, whole genome shotgun sequence.
ACCESSION   DATNAE010000272 DATNAE010000000
VERSION     DATNAE010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 8448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8448
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3877187"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="VN518_06090"
     CDS             complement(<1..609)
                     /locus_tag="VN518_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015316990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXE04179.1"
                     /translation="MVQPFLPGFRTEDIETGGATIHVRFGGSGPAVVMLHGFADTGDM
                     WAPLAAELAKDHTVIVPDLRGIGLSSHPEGGYDKKTQAGDVARVLDRLGIEKADLVTH
                     DIGNMVGYSFAAQYPERVTRWVAMDAPLPGIGNWDEIVKSPLLWHFNFRGPDVERLVA
                     GRERIYLDRFLNELSANPASIDEATRRHYAKLYALPGAMHSAF"
     gene            complement(617..1630)
                     /locus_tag="VN518_06095"
     CDS             complement(617..1630)
                     /locus_tag="VN518_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012253753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase family peroxidase"
                     /protein_id="HXE04180.1"
                     /translation="MHAKQALSILSLALTLVAAAPAFAEDAADQKSLPTELIDALNAV
                     FGKQTDNRAVHAKGIVLEGKFTPSAEAASLSKAPHLQAGPTVPVTVRFSDFAGVPAIP
                     DTDPNASPRGMAVKFHLPDGSNSDLVMHSYNGFPTATAAEFRDLLLALAASGKDAPKP
                     TPLETFFGTHPIAKTFLTSEKPGPVGFATLPYFGVNSFKFTNAKGDSTVVRYQMLPEG
                     GAEYLTKEQLAEAGPNYLADEIRKRVGDGPVRMKLQVQIAEPGDKIDDPSIAWPDSRK
                     TVELGVIEIDKVDPDSDAAQRALLFLENAVPAGIETADPMIDIRSQAYAVSFGRRHAT
                     AAQ"
     gene            2101..2373
                     /locus_tag="VN518_06100"
     CDS             2101..2373
                     /locus_tag="VN518_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE04181.1"
                     /translation="MFKAYIMPALAGAFLLGLATAAWAVKGEFGNMCAMSLAAGKDTQ
                     TDCSVNAQLQGKTYCFGSKEAMNEFMRNPKDNLAKAQSYYSSKHPG"
     gene            complement(2439..2906)
                     /gene="bcp"
                     /locus_tag="VN518_06105"
     CDS             complement(2439..2906)
                     /gene="bcp"
                     /locus_tag="VN518_06105"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003599311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HXE04182.1"
                     /translation="MSLEIGDKAPSFNLPADGGGKIALKDLKGKKVVLYFYPKDDTSG
                     CTAEACAFRDSLPDFSKVKAAIVGISRDSVESHDKFKKKFHLTFPLVSDEDGKVCEAY
                     GTWVEKSMYGRKYMGIERSTFLIDEKGVIRGIWRKVKVPGHADELLKAAEALS"
     gene            complement(2937..3365)
                     /locus_tag="VN518_06110"
                     /pseudo
     CDS             complement(2937..3365)
                     /locus_tag="VN518_06110"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(3362..5728)
                     /locus_tag="VN518_06115"
     CDS             complement(3362..5728)
                     /locus_tag="VN518_06115"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HXE04183.1"
                     /translation="MLYIDVGNTLRGGLRTGIQRVVRSLSRELAVNAPSKTRLIAFDV
                     AAGRYFALGDPELIQSAGSLTDIGREARVYFDFADFAQGDIFFEPDSSWTEPLNRGAL
                     FRRLKAQGVIVVVLNHDAIPIILPEVCHPNTLVSFSETIADHLQYADYALATSAGVAR
                     DLGKVSERFLSRSMAMRVIKLGADFAPPGSADEGDLFAARFPELAPLRFLLSVGTIEP
                     RKNHALLLQAFDRLAAKDAGLVIVGRKGWMADDTLAALTGHPDFGKRVFWLTALDDQA
                     LLSLYRHAYASVLPSQYEGYGLPVVEALSQGCVTVASDAGSLPEVAAGHAVFFRRGDD
                     EALYAILDRLYGDRDYYARLKASARSFSPTPWREAGRTVAAALADIASGASHDFAAPL
                     RQMVFLSVHAERLDLALQSARANLGFIDRIAVLTTPAAREAIAAVAAQHFPKALVLTD
                     DVVAGEKLPADHQARNTWLRKRLYVQDAIEANFLAADEDSLALRPLTRAYYQTDDVHT
                     AYSFQDDMGTWLAGSPTTTSFDRGMRNAWKLLAEAAYPVRAYASHMPQIVNKSLANEI
                     FARFVISPDGPAYDEWSLYFNVAQQLHPRQFATKPYGALGWPMRPGDWLPEFEPMQPA
                     FENYYPQNYESAERGMFVGLNPLGDLDAKASRSREALAGARRAETKGGGPHSPGLLAL
                     ILTPEALKFVGSGTILAGMRNVRRVLLVNGSSDVTLTTGRLDMFVTEPRGTPVTGESV
                     RLGEVCWIPLLPPDKPGLYAIRFFAALDSGARLEARGLLTVVANGKRQ"
     gene            complement(5741..6298)
                     /locus_tag="VN518_06120"
     CDS             complement(5741..6298)
                     /locus_tag="VN518_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE04184.1"
                     /translation="MEAAKPSALYRLAKRLSLGRLARYFALWARYLLRRGQSALSTKL
                     LREPPSGIDAQAAALAGAELASAKRMTVASWFPLDLANARLHRALLASVAGARADLKL
                     TPEILDCFVSLKSADDLAACIEGMVEQGRSPQTVNTALYVLLLRRLPASSELAMIASR
                     HPRHALIAIQSGDEYRKQGRRARVG"
     gene            complement(6313..7365)
                     /locus_tag="VN518_06125"
     CDS             complement(6313..7365)
                     /locus_tag="VN518_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(3-hydroxybutyrate) depolymerase"
                     /protein_id="HXE04185.1"
                     /translation="MSTRSWAFVGLSVVSLLGASATAATAEDLPKLGVTLPATSVSGL
                     SSGAYMAGQIEVAHSKDIVGAGIVAGGPFACAETASSELFPYWPIVVWQNATQAANSC
                     MKDDWGAPDADKLAKRAKELAEDGKIDDLSGLADDKIYLFSGNEDQTVVRPVVEAAKR
                     FYEAAGMPAGSVTLVEKEGGHAFLTETEGTACGLSKDPYVSDCDYDQAKAILEWIYGQ
                     PLSDPSTSPKGKFITFDQSPFNNGVPNGLAADGVVYVPEDCVSHTGCRLHITLHGCDQ
                     ARETVGDAFIKESGFAHYADTNRLVVLFPQIAGSVMNPHGCWDWWGYSDIDYLGKDAP
                     QIKAVWAMAERLAMQP"
     gene            7551..8087
                     /gene="speD"
                     /locus_tag="VN518_06130"
     CDS             7551..8087
                     /gene="speD"
                     /locus_tag="VN518_06130"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HXE04186.1"
                     /translation="MSDDALFQLGMDLDRGSSTAQTEEKLSRCRSSREDERKDFFCER
                     DGVRYAGTHLIIDLVRAERLDDLEHIEATLRRCVEVAGATLLHIHLHHFTPNGGVSGV
                     AVLAESHISIHSWPEYGYAALDVFMCGQTHPRRAIDVLKEAFSPRKVVVKEHLRGKEM
                     NRWTGGSKRRYTAASASV"
     gene            8036..>8448
                     /locus_tag="VN518_06135"
     CDS             8036..>8448
                     /locus_tag="VN518_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HXE04187.1"
                     /translation="MDRWVEETLHRGFRIRLKADGVLFDSETEHQQLIILETPEFGRV
                     MMLDGVIQLASKDEFVYHEMMSHVPLFAHGRARKALIVGGGDGGVLREALKHPELESV
                     TLCEIDRSVVELCREYFPEVSAGAYDNPRTRIVIA"
BASE COUNT         1549 a         2503 c         2864 g         1532 t
ORIGIN      
        1 gaaggccgaa tgcatggcgc cgggcaacgc gtaaagcttg gcgtagtgcc ggcgggtcgc
       61 ttcatcgatc gatgccggat tggccgacaa ttcgttcaag aagcggtcga gatagatgcg
      121 ctcgcggcct gcgaccagcc gctcgacgtc gggcccgcgg aaattgaaat gccagaggag
      181 gggactcttc acgatctcat cccaatttcc gatgcccgga aggggagcgt ccatggctac
      241 ccagcgcgtg acgcgctcgg ggtattgggc ggcgaaggaa tagccgacca tgttgccgat
      301 gtcgtgggtc acgagatcgg ccttctcgat gccgaggcgg tcgagcacgc gggcgacatc
      361 gccggcttgg gttttcttgt catagcctcc ttccgggtgg gaggagaggc cgatgccgcg
      421 caagtccggg acgatgaccg tatggtcttt cgcgagctcc gccgccaagg gcgcccacat
      481 atctccggtg tcggcgaagc catgcagcat gacgacggcc ggccccgagc caccaaaacg
      541 cacatggatg gtggcgccgc cggtctcgat gtcctcggtg cggaagccgg gcaggaaggg
      601 ctgaaccaac ttcgattcac tgcgccgcgg tcgcgtgacg gcggccgaag gagacggcgt
      661 aggcctgact gcggatatcg atcatcgggt cggcggtctc gatgccggca gggacggcgt
      721 tctcgaggaa caacagcgcg cgctgggcgg cgtcgctatc aggatcgacc ttgtcgatct
      781 cgatcacgcc aagctccacg gtcttgcggc tatcaggcca agcgatcgac ggatcgtcga
      841 tcttgtcgcc aggctcggcg atctggactt gcagcttcat gcgcaccggt ccgtcgccga
      901 cgcgcttgcg gatctcatcc gcgagataat tgggaccagc ctcggcaagc tgctccttgg
      961 tgagatactc ggcaccgccc tccggcagca tctgatagcg gacgacggtg ctgtcgccct
     1021 tggcgttggt gaatttgaag ctgttcacgc caaaataggg gagcgtggca aagccgaccg
     1081 gccccggctt ttcagaggtc aggaaggtct tggcgatcgg atgggttccg aagaatgtct
     1141 cgagcggcgt cggcttcggc gcgtctttgc cgctagcggc gagcgcgagc aggagatcgc
     1201 ggaactcagc ggcagtggcg gtgggaaagc cgttataaga gtgcattacc aggtccgaat
     1261 tcgaaccgtc cggcagatgg aatttcaccg ccatgccgcg cgggcttgca ttcgggtcgg
     1321 tgtcagggat cgccggcacg ccggcaaaat ccgagaagcg gacggtgacg ggcacggtgg
     1381 ggccggcctg aaggtgcggc gccttgctca aagaggcagc ctcggcgctc ggggtgaatt
     1441 tcccttcgag cacgatgccc ttggcgtgca ccgcccggtt atcggtctgc ttgccgaaca
     1501 cggcgttgag cgcgtcgatc agttcggtgg gcaacgattt ctgatcggca gcgtcttcgg
     1561 cgaaggcggg ggctgccgcc acgagcgtca acgccaacga caaaatggaa agtgcttgct
     1621 tcgcgtgcat gatgggctcc tttcgcaggg tgcaccctat gatcccgcga gaacgtagcc
     1681 gttttgcgaa ttgcgcgggc tttttgaacc cgcccgctcc gaggtcaaag tgccgcgccc
     1741 ccggcccaaa gtggtatcgc atcgcaatgt cgcatgcggc ggcggttgga cttggctctc
     1801 gtgtggcctg aaattcggca gttaccggcg cgttcgcgag agcggtcggc atggcccctg
     1861 cgcctggctg cgggcacaca agaattgtgc ggaaaatgca ttcgcatcag aacgcaaaat
     1921 tcacgacaat tttacgaaag aagcgaacgg atcgcgaggc taacgcccga acctgagaag
     1981 tccgaggcag attgcgccgg actgaggcca aattgcacat gggagcgtta caaaaactgc
     2041 gcatgagagc gttaagtgtg tctcgggccg atcccctaaa ggcccaccaa ggaggttacc
     2101 atgttcaagg cgtacatcat gcccgcgctg gcgggcgcat tcctgcttgg cctggctacg
     2161 gctgcttggg ccgtaaaggg cgagttcggc aacatgtgtg cgatgagtct cgcagccggt
     2221 aaagacacgc agaccgactg ctcggtcaac gcgcagcttc agggcaagac ctactgcttc
     2281 ggcagtaagg aggccatgaa tgagttcatg aggaacccca aggacaatct cgccaaggcg
     2341 cagtcctact attcatcgaa gcatcccggc taggctgggc gcttcgcaaa agcatcgcaa
     2401 gctctccgcc gatggcggag ggcttgtgtg cgtctgagtt agctcagcgc ctcggccgcc
     2461 ttcagcagct cgtcggcgtg gccaggcact ttcactttgc gccagatgcc gcggatcacg
     2521 cccttctcgt cgatgaggaa ggtggagcgc tcgatgccca tatatttcct gccgtacatg
     2581 ctcttttcca cccaggtgcc gtaggcctca cacactttgc cgtcctcatc ggagacgagc
     2641 gggaaggtga ggtggaactt ctttttgaac ttgtcgtggc tctcgaccga gtcgcgcgag
     2701 atgccgacga tcgcagcctt caccttggag aagtcgggga gagagtcgcg gaaggcgcaa
     2761 gcctcggcgg tgcagccaga ggtgtcgtcc tttggataga aatagagcac gacctttttg
     2821 cccttgagat ctttgagcgc gatcttgccg ccgccatcgg ccggcagatt gaaactcggg
     2881 gccttgtcgc caatttcgag gctcatgcgt ttccctccta aggtccgctt ggtcattctg
     2941 atatgcggga tggtgttctc gtcgaagggc tcgccctcta ttgtatagcc gtgcttctgg
     3001 tagaacgcct ccgcgccgag ccgcgcgttg aggatgatca tgccatagcc gccgcgccgc
     3061 gcccaagcct cggcgtagct gagcagccgc gagccgatgc cctcgccctg cttgccgtca
     3121 gccaccgcca tctgcttgag ctgcagcgtg tgcgcgccga gccgcttgaa cgacaccgat
     3181 cccaccacgc tgccgttgag ggtggcgcag aaatgcctgc gatgagcatc gtcggccaac
     3241 tcttccgcgc tgagggtaag gccgagaggc ctgcgcagga tggcctcgcg caagcgcaaa
     3301 gcctgggcgt agagggccga gccgtatggc gcctcggaga ggtgaatgtc gtcggcctcg
     3361 gtcactggcg cttcccgttg gcgaccacgg tcaacaggcc tcgcgcctcc agcctcgcgc
     3421 cgctgtcgag ggcggcgaag aagcggatgg cataaaggcc aggcttgtcg ggaggcaaga
     3481 gcgggatcca gcagacttcg cctaagcgca cgctttcgcc ggtgacaggg gtgccgcgcg
     3541 gctcggtgac gaacatatcg agtctgcctg tcgtgagggt gacatcggag gagccgttga
     3601 cgaggagaac gcgccgcaca ttgcgcattc cggcaaggat ggtgcccgag cccacgaatt
     3661 tcagcgcctc cggcgtgagg atcaaggcaa gcaggcctgg tgaatgcggg ccgccgccct
     3721 tggtctcggc gcggcgggct ccagccaggg cctcccggct gcggctcgcc ttcgcgtcga
     3781 gatcgccgag agggttgagc cccacgaaca ttccgcgctc ggcgctctca taattctggg
     3841 gataataatt ttcgaaggcc ggctgcatcg gctcgaactc ggggagccaa tcgcctggcc
     3901 gcatcggcca gccgagcgcg ccatagggct tggttgcgaa ctggcgcggg tgaagctgtt
     3961 gggcgacgtt gaagtaaagc gaccactcgt catacgcagg tccgtccggg ctgataacga
     4021 accgggcgaa gatctcattg gcgaggctct tattgacgat ctgcggcatg tgactggcat
     4081 aggcgcgcac agggtaggcc gcttcggcga gaagcttcca ggcgttgcgc atgccgcggt
     4141 cgaagctcgt cgtcgttggc gagccggcaa gccaggtccc catgtcgtcc tggaaggaat
     4201 aggcggtgtg cacgtcgtca gtctgataat aggcgcgggt cagcgggcgc agcgcgagcg
     4261 agtcctcatc ggcggcaagg aagttcgcct cgatagcgtc ttggacgtag agccgcttgc
     4321 gcagccatgt gttgcgcgcc tggtgatcgg caggaagctt ttcgcccgcc accacatcgt
     4381 cggttaggac aagcgccttg gggaagtgct gcgcggccac ggcggcgatc gcctcgcgcg
     4441 cggcaggcgt ggtcagcacg gcgatgcggt cgatgaagcc gagattggcg cgcgccgatt
     4501 gaagcgcaag atcgagcctc tcggcgtgca ccgacaggaa caccatctgc cgcaacggcg
     4561 cagcgaaatc gtgcgaggcg cccgaggcga tgtcggcgag cgccgccgcc acggtccggc
     4621 cggcctcgcg ccagggcgtc gggctgaagc tcctggctga cgctttcagg cgagcgtaat
     4681 agtcgcgatc tccataaagc cggtcgagga tggcataaag cgcttcgtcg tcgccccggc
     4741 ggaagaacac ggcgtggccg gccgccacct cgggcaatga gccggcgtcg gaggccacgg
     4801 tgacgcatcc ttgcgacagg gcctcgacca cggggaggcc gtagccttcg tattgcgacg
     4861 gcagtaccga cgcataagca tggcggtaga gggaaagcag cgcctggtcg tcgagcgcgg
     4921 tcagccagaa gacgcgtttg ccgaagtcgg gatggccggt gagcgccgcc agcgtgtcgt
     4981 cagccatcca gcctttgcgg ccgacgatga cgaggcctgc gtccttggcc gcgagccggt
     5041 cgaaggcctg aagcagaagc gcgtgattct tgcgcggctc gatggtcccg accgagagga
     5101 ggaagcgcaa aggcgcaagc tcggggaagc gggcggcgaa gaggtcgcct tcatcggcgc
     5161 tccccggcgg ggcgaaatca gcgccgagtt tgatgacgcg catggccatg gagcgggaaa
     5221 ggaagcgctc ggacaccttg ccgaggtcgc gggcgacgcc ggccgaggtg gcaagcgcat
     5281 agtcggcata ttgcaggtgg tcggcgatgg tctccgagaa ggagacgagc gtattgggat
     5341 ggcaaacctc cggcaggatg atcgggatgg cgtcgtgatt gagcaccacg acgatgacgc
     5401 cttgcgcctt gaggcggcgg aacaacgcgc cgcggttgag cggctcggtc cagctcgaat
     5461 ccggctcgaa gaagatgtcg ccttgggcaa agtcagcgaa gtcgaaatag acgcgggcct
     5521 cgcggccgat gtcggtcaag ctgcctgccg attggatcag ctcagggtcg ccaagcgcga
     5581 aatagcgccc ggcggccacg tcgaaggcga tcaggcgcgt cttgctcggc gcattgacgg
     5641 caagttcacg actgagcgag cggacaacgc gctggatgcc ggtcctgagg ccgcctctga
     5701 gcgtgttgcc gacgtcgata tagagcatgc ggccccctgg tcagcccacg cgggcccttc
     5761 ggccctgctt gcgatattca tcgccggact gaatggcgat cagggcatgg cgcggatggc
     5821 gcgaggcgat catggcaagc tcggaggagg ccgggaggcg cctgagcagg aggacataga
     5881 gagcggtgtt gacggtttgc ggggatcggc cttgctcgac catgccctcg atgcaggccg
     5941 caagatcatc cgcgctcttg aggctgacga agcaatcgag gatctcaggc gtgagcttaa
     6001 gatcggcacg ggcaccggcg acgcttgcga gcagcgctcg gtgcagccgg gcattggcga
     6061 gatcaagcgg aaaccatgag gcaaccgtca tgcgcttggc gctcgccaac tccgctccgg
     6121 cgagtgctgc agcttgcgcg tcgatgccgc tcggcggctc cctcagtagc ttggtggaaa
     6181 gggcgctttg gccgcggcgc aacagataac gcgcccaaag agcgaagtag cgcgcgagcc
     6241 gcccgagcga gaggcgcttc gcgagccggt agagcgcgct tggcttggcc gcctccatgg
     6301 gtgggcgccc tatcaaggct gcatcgccag gcgctcggcc atggcccaca cggcctttat
     6361 ttgcggggcg tccttgccga gatagtcgat gtcgctatag ccccaccagt cccagcagcc
     6421 atgcgggttc atcacactcc cggcgatctg cgggaacagg acgacgagcc ggttggtgtc
     6481 ggcgtagtgg gcaaagccgc tttccttgat gaaggcgtcg ccgacggtct cgcgcgcctg
     6541 gtcgcagcca tgcagcgtga tgtgcaagcg gcaacctgta tgactcacgc aatcctctgg
     6601 cacatagacc acgccgtcgg cggcgagccc gttcgggacg ccattgttga agggtgattg
     6661 gtcgaaggtg atgaacttgc cctttgggct ggtcgacgga tcagacaggg gctggccata
     6721 gatccattcg aggatcgcct tcgcctgatc gtaatcgcaa tcgctgacat aaggatcctt
     6781 ggagaggccg caagccgtgc cttcggtctc ggtcaggaag gcgtggcctc cttctttctc
     6841 gaccagggtg acgcttcccg ctggcatgcc cgcagcttca tagaagcgct tggcggcctc
     6901 gaccacgggg cggaccacgg tctgatcttc gttgcccgag aacagataga tcttgtcgtc
     6961 ggcgaggccg gagagatcgt cgatcttgcc gtcctcggca agctccttgg cgcgcttggc
     7021 gagcttgtcg gcgtccggcg cgccccagtc gtccttcatg caggaattcg cggcttgcgt
     7081 ggcgttctgc cacacaacga tgggccaata gggaaaaagc tcgctggagg ccgtctcggc
     7141 gcaggcaaag ggtccgccgg cgacgatgcc ggcgccgacg atgtccttgg agtgggcgac
     7201 ctcgatctgt ccggccatat aggcgcctga ggagagcccc gagacggaag tggcgggcag
     7261 cgtgacgccg agtttcggca ggtcttcggc cgtagcggca gtggcgctgg cgccgaggag
     7321 agagaccacc gagagaccga cgaaagccca gctgcgcgtc gacatcagcc tcggttcctc
     7381 cctttgcggg atttgtgacg ggcgtctctc gtccgctccg ggagacgcgc agataacttt
     7441 attaggtcct gaagtgcctt tgggccaaaa acttcttgca tcgctgcagc atgggtgtac
     7501 atagcgccgt cgtttaactt gaaacatctg ctggttggag ggaggacgcc atgtccgatg
     7561 acgccctctt ccaattgggg atggacttgg atcgtggttc aagcaccgcc caaacggaag
     7621 aaaagctttc tcgttgccgc tcctcgcgag aggacgagcg taaggatttt ttctgtgagc
     7681 gtgacggggt tcgctacgct ggcacccatc tcatcatcga cctggtgcga gcggaacggc
     7741 tcgacgatct agagcacatc gaagcgacgc ttcgacgctg cgtcgaagtc gctggagcca
     7801 cgctcttgca catccacctc catcacttca cgccaaatgg cggagtgtcg ggcgtggcgg
     7861 tgcttgccga atcgcatatc tcgattcata gctggcctga atacggctac gccgccctcg
     7921 atgtcttcat gtgcggccag acccatccac gacgcgccat cgatgtattg aaagaggcgt
     7981 tctcgcctcg caaggtggtg gtcaaggagc atctgcgcgg caaggagatg aatcgatgga
     8041 ccggtgggtc gaagagacgt tacaccgcgg cttccgcatc cgtctgaagg cggacggcgt
     8101 cctcttcgac agcgagaccg agcatcagca gctgatcatc ctggagaccc ccgaattcgg
     8161 gcgggtgatg atgctcgatg gggtgatcca gctcgcctcg aaggacgagt tcgtctacca
     8221 cgagatgatg agccacgtgc cgctgttcgc ccacgggcgg gcgcgcaagg cgctcattgt
     8281 cggcggcggc gacggcgggg tcttgcgcga ggcgttgaag catcccgagc ttgagagcgt
     8341 cactctgtgc gagatcgacc ggagcgtggt cgagctctgt cgcgagtatt ttcccgaggt
     8401 gtcggccggc gcctatgaca atccgcggac gaggatcgtc atcgccga
//
LOCUS       DATNAE010000273         3623 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2376899, whole genome shotgun sequence.
ACCESSION   DATNAE010000273 DATNAE010000000
VERSION     DATNAE010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2376899"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="VN518_06140"
     CDS             <1..398
                     /locus_tag="VN518_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXE04188.1"
                     /translation="ASGDIDVIVSAHDPRGAEGKRRPFAEAADGAVGLETLLGAALRL
                     YHNGEVELGRLLQALTVNPARLLKLGGGRLAKGAPADLVLIDLDRPWVVEPEALRSKS
                     KNTPFDAARLQGRALLTFVAGRLVYNYAE"
     gene            426..1055
                     /gene="plsY"
                     /locus_tag="VN518_06145"
     CDS             426..1055
                     /gene="plsY"
                     /locus_tag="VN518_06145"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HXE04189.1"
                     /translation="MPPSEAWEQALPLLLALCFLSFGFGYLLGSIPFGLILAKLAGLG
                     DLRQIGSGNIGATNVLRTGNKALAALTLLLDLLKGTAAVWVGSHFGPYAAMAAGLGAF
                     LGHLFPVWLGFHGGKGVATYLGVLLGLYWPAAAAFSVVWLLVAFATRYSSLAALVASA
                     ASVGMLVLTGQWRLAGLFLLLTLLLFIRHAANIGRLAKGEEARIGERTV"
     gene            1105..2195
                     /gene="dprA"
                     /locus_tag="VN518_06150"
                     /pseudo
     CDS             1105..2195
                     /gene="dprA"
                     /locus_tag="VN518_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214671.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
     gene            complement(2207..2416)
                     /locus_tag="VN518_06155"
     CDS             complement(2207..2416)
                     /locus_tag="VN518_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE04190.1"
                     /translation="MAGSRARPPAQGPSSSSHGQDLTHQEAAEYIASLLEGLKQLAHS
                     ARMPFLAYLITVALEEANNEKTHGG"
     gene            2681..>3623
                     /locus_tag="VN518_06160"
     CDS             2681..>3623
                     /locus_tag="VN518_06160"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HXE04191.1"
                     /translation="MNIVIVESPAKAKTVNKYLGAGYRVFASYGHVRDLPSKNGSVLP
                     DKDFEMHWDVEPKAAKRLDEIAKAVKGASKLILATDPDREGEAISWHVLQVLDRKKAL
                     AGVPVERVVFNAVTKEAVLDAMRHPRAIDGPLVSAYLARRALDYLVGFTLSPVLWRKL
                     PGARSAGRVQSVALRLVCDRELEIEKFRAQEYWSILAELATTKNETFQARLVAADGKK
                     LDKFDIPDQATAERLKSALEGGRFKVASVESKAQRRHPAAPFTTSTLQQEASRKLGFS
                     PRHTMQLAQRLYEGVDMGGEAEGLITYMRTDGVQIVPE"
BASE COUNT          589 a         1226 c         1137 g          671 t
ORIGIN      
        1 tggcgagcgg cgatatcgac gtgatcgtct cggcgcacga tccgcgcggc gccgagggca
       61 agcggcgccc gtttgccgag gccgccgacg gagccgtggg gctcgagacc ttgcttggcg
      121 ccgcgcttcg tctctaccac aatggcgagg tcgagcttgg gcggctcttg caagcgctca
      181 cggtaaatcc ggcgcgtttg ctcaagcttg gcggcggccg cctcgccaag ggcgcgcccg
      241 ccgatctcgt gttgatcgat ctcgaccggc cttgggtggt cgagccggaa gcgttgcggt
      301 caaaatcgaa gaacacgccc ttcgatgcag ccaggcttca gggtcgggcc ctgctgacct
      361 tcgttgccgg tcgcctcgtc tacaattacg ctgagtaatc tgccggcgtt caaagagggt
      421 gatcgatgcc gccttctgag gcttgggaac aagccctccc gttgctgctc gccctttgtt
      481 tcttgagctt cgggttcggc tatctgctcg gttccatccc cttcggactc atccttgcca
      541 agctcgccgg gctcggcgat ttgcggcaga tcggctccgg caatatcggc gcgaccaatg
      601 tcttgcgcac aggcaacaag gccctggcgg cgctcacgct gctgctcgat ctgctgaaag
      661 gaaccgccgc cgtatgggta ggatcgcatt tcggccccta cgccgccatg gccgctgggc
      721 taggcgcttt tctcggccat ctctttccgg tgtggctcgg ctttcacggc ggcaaaggcg
      781 tggcgaccta tctcggcgtg ctgctcggcc tctactggcc tgccgccgcg gcgttctctg
      841 tcgtctggct gctcgtcgcc ttcgccacac gctattcctc cctcgccgcg ctggtggcga
      901 gcgcggcaag cgtcgggatg ctcgtgctga ccgggcagtg gcggctcgcg ggcttgttcc
      961 tgctgcttac gctgcttctc tttatccggc acgccgcaaa tatcgggcgc ctggcgaagg
     1021 gcgaggaggc gcgcattggc gagaggaccg tctaagaccg cgcccaagca gaaaggcgcg
     1081 ggccggctca accatcatca gcgcatcgcc tggctcaggt tgatcagaag cgagaatgtt
     1141 gggcccgcca ccttccgtgc tctggtcaat gagttcggcg gcgccgaggc cgccatcgac
     1201 gcgctgccgc ttctgtctcg gcgcggagga cgctccgaca tccgtctctg caccgcggcc
     1261 gaagccgagg ccgagctcgc ggcggcggat cgcctcggcg caagcctcgt tgccgtcggc
     1321 gagcccggct atccgccggc acttgcccaa gtcgatgcac cgccgccgct gctttatgcc
     1381 aaaggcaggc tcgatctcgc caatattccc atcgtcgcca tggtcggcgc gcgcgcaacg
     1441 gttcggcggt cgggcagaaa ttcacccgtc agatcgccac cgagctcgcg ctcgaaggtt
     1501 tcgtcatcgc ctcaggactc gcccgcggca tcgacacggc ggcgcacatg gcagccttgg
     1561 agcatgggac catcgccgtg ctggctggcg gcatcgacat cgtgtatccg cccgagaatg
     1621 aagagttgca gcacgcgatc ggcgagcgcg gcctcttgat cagcgagcgc tctcccggct
     1681 tctctccgcg cggcaaggat tttcctcgtc gcaaccggct gatctcgggg atctccctcg
     1741 gtgtggtggt gatcgaggcc gccgagcgct ccggctcgct gatcaccgcc cgcttcgccg
     1801 gcgaacaggg acgcgaggtc ttcgccgtcc ccggcagccc gctcgatccg cgggcggcgg
     1861 ggaccaacaa tctcctgaag cagggcgcct gtctcgtcac ctcggcgcgc gatattctgg
     1921 aaacgctcgc gccaatcctc ggccggcccc ctgccccgcc gccgatcgag cttgctgccg
     1981 ccgacgagca tcggccgccc gagccgctgc ccgacattcg ccaaagcgaa cgcgagttgg
     2041 tcgtgtcggc acttggtcca agcccggtcg atatcgacga gctgatccgc gccaccggcg
     2101 tgacgacgag aaaggttcat atcgtgctgc tcgagctcga cctggcaggc aggttgcaac
     2161 ggcatggtca gcagctcgtg tcgctgaagg tctgacggcc tggagcttac ccgccgtggg
     2221 tcttctcgtt attggcttcc tcgagcgcca cggtgatgag gtaggccagg aacggcattc
     2281 gcgcgctatg ggcgagctgc ttcagcccct cgagcaacga ggcgatatat tccgctgcct
     2341 cctggtgcgt caggtcttgc ccatggctcg atgagcttgg accttgcgct ggaggccgag
     2401 cgcgcgaccc tgccattccg tatcgcccta ttttcgcgcc gattggaccc cagcaccgcg
     2461 ccgaaacgtt gaatctccgg ccacgggcgc ttcccaagac agttctatca gcaacagctt
     2521 aaggttgttt tagttagtat atacacctat aagttgtcaa ttgggttcac ccgaatttga
     2581 cacttggcgg tctagacacc actttccggg cgctaagccg agtccctccg gactcacccg
     2641 ccctcagcct gacgcaattt gcgcggctag gaacgtctca atgaacatcg tcatcgtcga
     2701 atcgccagcc aaggccaaga ccgtcaacaa gtacctgggg gcgggttacc gcgtgttcgc
     2761 gtcatacggg catgtgcgcg atcttccctc aaaaaacggc tccgtcctgc cggacaagga
     2821 cttcgagatg cattgggacg tcgagcccaa ggccgccaaa cgcctcgacg agatcgccaa
     2881 ggccgtcaaa ggggcgagca agctgatcct ggccaccgac cccgaccggg agggtgaggc
     2941 catctcctgg cacgtcctcc aggtgcttga ccgcaagaag gcgctcgccg gggtacccgt
     3001 ggagcgggtg gtcttcaacg ccgtcaccaa agaggccgtg ctcgatgcca tgcggcatcc
     3061 ccgcgccatc gatgggccac tggtcagcgc ctacctcgcg cggcgggcgc tcgattacct
     3121 tgtcggcttc acgctctcgc ccgtgctgtg gcgcaagctg ccgggcgcac gctccgccgg
     3181 gcgggtgcaa tcggtcgcgc tgcggctggt ctgcgaccgc gagctcgaaa tcgagaaatt
     3241 cagggcgcag gaatactggt cgatcctggc ggagctcgcc accaccaaga acgagacctt
     3301 ccaggcgcgt ctcgtcgccg ccgacggcaa gaagctcgac aagttcgata ttcccgatca
     3361 ggcgaccgcc gaacgcctga agtctgcgct ggaaggcggc cgcttcaagg tcgcgagcgt
     3421 ggaaagcaaa gcgcagcgcc gtcacccggc cgcccccttc accacctcga ccctccagca
     3481 ggaggcatcg cgcaagctcg gcttctcgcc gcgccacacc atgcaactcg cccagcgact
     3541 ttacgagggc gtcgatatgg gcggcgaggc cgaaggcctc atcacctata tgcgaacaga
     3601 cggcgtgcag atcgtgccgg aag
//
LOCUS       DATNAE010000274         3343 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_1466436, whole genome shotgun sequence.
ACCESSION   DATNAE010000274 DATNAE010000000
VERSION     DATNAE010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1466436"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..926
                     /gene="trmFO"
                     /locus_tag="VN518_06165"
     CDS             <1..926
                     /gene="trmFO"
                     /locus_tag="VN518_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009451733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HXE04192.1"
                     /translation="AFFDAIAPIVHRDTIDMNVCWLQSRYDKEGPGGGTADYINCPLD
                     KAQYEAFIDALISGEKTSFKEWEASTPYFEGCLPIEVMAERGRETLAYGPMKPVGLTN
                     PHRPERPYAVVQLRQDNALGSLWNMVGFQTKLKHAEQVRIFHTIPGLEQAEFARLGGL
                     HRNTFLNSPVLLDQSLRLKADTRLRFAGQITGVEGYVESAAIGLLAGRFAAAERLGTP
                     LSLPPPTTALGALLNHITGGHLASGGDGTSRSFQPMNVNFGLFPPLAAPPPRVKSERG
                     SKTLARKHALATRSLADLERWLAGGTSIAAE"
     gene            complement(981..1319)
                     /locus_tag="VN518_06170"
     CDS             complement(981..1319)
                     /locus_tag="VN518_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE04193.1"
                     /translation="MSAAVSRFPLGVRGAIPPQTVLVDGLGTVRLEVAGNSLTASIES
                     GPELRFDLTEAVNRLSGVVSTERRPLPIEAVNGALSGTVLIENLYGTFDGADLDLSLL
                     RLWLVLKRGN"
     gene            complement(1382..2239)
                     /locus_tag="VN518_06175"
     CDS             complement(1382..2239)
                     /locus_tag="VN518_06175"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HXE04194.1"
                     /translation="MQAGDRDAAVRTIARDGDLDRYVSVLFAPGARREHLFALYAFNV
                     ELARIGEQVSEAQLGEIRLQWWRDAFDRASSGETTGQPVADAIGFAVRECKLSQQSLA
                     GLIDARRFDVSVKIMPDTAALDDYLANTAGALFNLASEVCMAGGRAAEPRALDEAVRA
                     AGIAYGLTGLMRALPVHLSLGRVDIPEDSLLRHGTSPAQLLAGEESEGLTELLADLRE
                     TARGALGSARQHVAELPPAARPAFLPLALVDPYLRALQNVDPLHQVADINPLTRLWRL
                     ATCRFPQSA"
     gene            complement(2275..3225)
                     /gene="secF"
                     /locus_tag="VN518_06180"
     CDS             complement(2275..3225)
                     /gene="secF"
                     /locus_tag="VN518_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HXE04195.1"
                     /translation="MFTGIHFIPADTKIGFVRLRTISWVISALLTVVPLLLVWKVGLN
                     FGIDFQGGTLIEIQTKESVADLADIRAKVHGLNLGEVQIQEFGAPNDVLIRVAAQPTE
                     KEQQESIAKVKQVLGDQVEYRRIEVVGPTISSELIAGGTVAVVVAMIAILMYIWFRFE
                     WQFAVAAIASLVHDVTGTIGLYSLLQLEFNLSSIAAILTIIGYSLNDKVVIFDRIREN
                     LRKYKKMPLTELLDLSINETLSRTVLTHVTTFLAMMPFVLVGGEAIFGFALAMVWGIV
                     IGAYSSIYVAAPLQLILGVKRDGGMASTPARAKTQARARA"
BASE COUNT          639 a         1092 c         1053 g          559 t
ORIGIN      
        1 ttgctttctt cgacgccatc gccccgatcg tgcatcgcga cacgatcgac atgaacgtgt
       61 gctggctgca atcgcgctac gacaaggaag gtcccggcgg cggcaccgcc gactacatta
      121 actgcccgct cgacaaggcg cagtacgagg ccttcatcga cgcgctcatc tctggcgaga
      181 agacctcgtt caaggagtgg gaggcatcga cgccttattt cgagggctgc ctgcccatcg
      241 aggtgatggc cgaacgcggg cgcgagacgc ttgcctatgg cccgatgaag cccgtcggcc
      301 tcaccaaccc gcatcgtccc gagcggccct acgctgtcgt gcaactcagg caggacaatg
      361 cgctcggcag cttgtggaac atggtcgggt tccagaccaa gctgaagcac gccgagcagg
      421 tccgcatctt ccacaccatt cccgggctcg agcaggcaga gttcgctcgg ctcggtggtc
      481 tgcatcgcaa taccttcctc aatagcccgg tgctgctcga tcagagcttg cggctgaaag
      541 ccgacacacg gctccgcttt gccggacaga tcaccggggt cgagggctac gtggaatcgg
      601 cggccatagg cctgcttgcc ggccgctttg ccgcagccga gcgcctcggt acgccacttt
      661 cgctgccgcc gcccaccacg gcgcttggcg cgcttctcaa tcacatcacc ggcgggcatc
      721 tcgcgagcgg aggcgacggc acctcgcgca gcttccagcc gatgaacgtc aatttcggtc
      781 tcttccctcc cctcgccgcc ccgccgccaa gggtaaagag cgagcgcggc agcaagacat
      841 tggcgcggaa gcatgcgctc gcgacgcgct cgctcgccga tctcgagcgc tggctcgcgg
      901 gcggcacctc gatcgccgcc gagtaggcgc ctcactcttt gggcgcccct gcggcaactt
      961 ccttccacgt gaagccgctg ctagttcccg cgcttgagca ccagccagag gcgcaacagc
     1021 gacaggtcga gatcggcgcc gtcaaaggtg ccgtagagat tttcgatcag gaccgtgccc
     1081 gacagcgccc cgttgaccgc ctcgataggc aaagggcggc gctcggtcga gacaacccct
     1141 gaaagcctgt tgaccgcctc ggtgaggtcg aagcgcagct cgggaccgct ctcgatactc
     1201 gcggtgaggc tgttgccggc aacttcaagc ctcaccgtgc cgagaccgtc gaccagcaca
     1261 gtttgcggcg ggattgcgcc tcgcactccg agggggaagc gcgaaacggc tgcgctcatg
     1321 tccggaagcc agctccgcca agaccagcgt cgtgttgcct cagtcatgga tggacctttt
     1381 cttacgccga ttgggggaag cggcaggtgg caagccgcca aagccgggtg agcgggttga
     1441 tgtcggcgac ctgatgcagc gggtcaacgt tctgcaaagc tctgagataa ggatcgacca
     1501 gagcaagcgg caggaaggcc ggtcgcgccg caggcggaag ctctgccaca tgttgcctcg
     1561 ccgatccgag cgcgccccgc gccgtctccc gcaaatccgc caagagctcg gtcagtccct
     1621 cgctctcctc tccggccaga agctgcgccg gtgaggtgcc atgacggaga agactatcct
     1681 cagggatatc gacgcggccg agactcaaat gaaccggcag agcgcgcatc agcccggtca
     1741 ggccataggc gatgccggcg gctctgacgg cctcatccag cgcgcgcggc tcggctgccc
     1801 ggcctccagc catgcaaacc tcggaggcga gattgaacag cgcgcccgcg gtgttggcga
     1861 gatagtcgtc gagcgccgcc gtgtccggca tgatcttcac cgacacgtcg aagcgcctcg
     1921 cgtcgatcaa accggcgagg ctctgctgcg acagcttgca ctcgcgaact gcaaatccga
     1981 tggcatcggc gaccggctgg ccggttgttt cgccgcttga ggcgcggtcg aacgcgtcgc
     2041 gccaccattg caggcgaatt tcgccaagct gagcctcgct cacctgctcg ccgatacggg
     2101 cgagctcgac attgaaggca tagagggcaa acagatgctc gcgccgggcg cccggcgcga
     2161 acagcacgct tacgtaacga tcgaggtcac catcgcgggc gatggtgcgg acggcagcgt
     2221 ctcgatcgcc tgcctgcaag gccttcccgt cctaagggac cggccgcgcc tcgctcaagc
     2281 gcgcgcccgg gcttgcgtct ttgctctggc cggcgtgctg gccatgccgc cgtcgcgctt
     2341 gacgccaagg atcagctgca acggagccgc cacataaatc gaggagtagg cgccgatgac
     2401 gatgccccac accatggcaa gcgcaaagcc gaagatcgcc tcgccgccga ccagcacgaa
     2461 cggcatcatg gcgaggaacg tggtcacatg ggtcaagacc gtgcgcgaca gtgtctcgtt
     2521 gatcgaaagg tcgagcagct cggtcagcgg catcttcttg tatttgcgca agttctcgcg
     2581 gatccggtcg aagatgacga ccttatcgtt gagcgaatag ccgatgatgg tgaggatggc
     2641 ggcgatgctc gacaggttga actcaagctg gagcaacgaa tagagcccga tggtgccggt
     2701 cacgtcatga accagcgagg cgatggcggc gaccgcgaac tgccattcga acctgaacca
     2761 gatgtacatc agaatggcga tcatggcgac gaccacggcc accgtgccgc cggcgatcag
     2821 ctcgctcgag atcgtgggcc ccaccacctc gatgcggcga tactcgacct gatcgccgag
     2881 cacctgcttg accttggcga tggactcctg ctgctccttc tcggtcggct gggcggcgac
     2941 gcggatcagc acgtcgttcg gcgcgccgaa ttcctggatc tgaacctcgc cgagattgag
     3001 cccatgcacc ttagcacgga tgtccgcaag atcggcgacg ctttccttgg tctgaatctc
     3061 gatgagcgtg ccgccctgaa agtcgatgcc gaaattgagc ccgaccttcc acacgagcag
     3121 aagcggcacc acggtcagca gggccgaaat cacccaactg atggtgcgta agcgaacgaa
     3181 gccgatcttg gtgtcggcgg ggatgaaatg aatgcctgta aacataacgg cgttttccta
     3241 gatcgggatc agggccggcc gcgcccagcg cagccaatag gacaccatga gccgcgtcag
     3301 ggtcacggcg gtgaacattg aggcgagaat gccgatggtg agg
//
LOCUS       DATNAE010000275         3039 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2677135, whole genome shotgun sequence.
ACCESSION   DATNAE010000275 DATNAE010000000
VERSION     DATNAE010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2677135"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /gene="tatC"
                     /locus_tag="VN518_06185"
     CDS             complement(<1..691)
                     /gene="tatC"
                     /locus_tag="VN518_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HXE04196.1"
                     /translation="MSREDEEKEIEATKAPLMEHLIELRSRLIKALIAFAIAFVGCFF
                     FAKTIYNVLTWPFVWVAGPENSKFIYTGLLEYFVVQLKLAMFGAAFISFPVVATQVYM
                     FVAPGLYRQEKQAFLPYLIATPIFFLLGALVVYILVWPMLARFSLNMQQMGGDGQATI
                     ELLPKVEDYLTLMMKLIFAFGIAFQLPVILTLLGRVGIITSDQLKEKRRYFVVGAFVI
                     AAVLTPPDVLSQ"
     gene            complement(688..1176)
                     /gene="tatB"
                     /locus_tag="VN518_06190"
     CDS             complement(688..1176)
                     /gene="tatB"
                     /locus_tag="VN518_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HXE04197.1"
                     /translation="MFEIGWGELLIIGIVALIAIGPKELPGVLRTLGQWMSKLRRMAS
                     EFQGQFQEAMREAELADLKKQVDDMTSQAQSYANFDPVGEVRRELESTQQQIESAMAE
                     KPMAETASPASPPVVAQSNAAPLGGEAGVAPVGDVAAPSAAEAEPAAAGSDPVRSDGK
                     PA"
     gene            complement(1257..1508)
                     /locus_tag="VN518_06195"
     CDS             complement(1257..1508)
                     /locus_tag="VN518_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HXE04198.1"
                     /translation="MGSLSIWHWILVLGVALLLFGGRGKISELMGDFAKGIKSFKKGL
                     TEEDKAETEPEKTDPKTIDHQPAPAPTATVTQPETRKAG"
     gene            complement(1776..2480)
                     /gene="scpB"
                     /locus_tag="VN518_06200"
     CDS             complement(1776..2480)
                     /gene="scpB"
                     /locus_tag="VN518_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HXE04199.1"
                     /translation="MPRLKVVVPDDEPDHAGEVRAEELRLLEAMLFASSEPLDEKSLA
                     ERLPQGVDVHAALLRLQEDYATRGVHLVRIGGKWTFRTAGDLSWLLSKETVETRKLSR
                     AAIETLAIIAYHQPVTRAEIEEIRGVTTSKGSVDVLLETGWIRPRGRRKSPGRPVTYG
                     TSEAFLSHFGLDAVNDLPGLDELKGSGLLDGRLPPSFSVPMPSDDPALREDEDPLEPG
                     DLDLGLAPPPERTGEE"
     gene            complement(2493..>3039)
                     /locus_tag="VN518_06205"
     CDS             complement(2493..>3039)
                     /locus_tag="VN518_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HXE04200.1"
                     /translation="EGQSAEDMAIALALRLKRLEAIRDVAERLFGRSQLDRDVFRRGH
                     PEPIAHIKHPQYSATLYDLLSAYAQQRQKTALAHVRLAKRTVWSLAEAREALERLIGQ
                     SSDWARLDEFLIAYVVEPSMAATVLASSFASTLEMVREGVMEVHQQGAFAPLYVRKRQ
                     QSGNGVADTGGVAAPSGDGKG"
BASE COUNT          534 a         1029 c          950 g          526 t
ORIGIN      
        1 gctggctcag cacgtcgggc ggagtgagca ccgccgcgat cacgaacgcg ccgacgacga
       61 agtagcgccg cttctcctta agctgatcgg aggtgatgat gccgacgcgc cccaacagcg
      121 tcaggatgac cggcagctgg aaggcgatgc cgaaggcgaa gatgagcttc atcatcaagg
      181 tcagataatc ctcgaccttc ggtagaagct cgatcgtggc ctgcccatcg ccgcccatct
      241 gctgcatgtt caaggaaaag cgtgccagca tcggccacac caaaatgtag accacgaggg
      301 cgccgagcag aaagaagatc ggcgtcgcga tgaggtaagg caggaacgcc tgcttctctt
      361 gacgatagag gccgggcgcg acgaacatgt agacctgcgt ggccacgacc gggaacgaga
      421 tgaacgcggc gccgaacatc gcaagcttga gctggacgac gaaatattcg agcaggccgg
      481 tataaatgaa cttcgagttc tccggccctg cgacccaaac gaacggccag gtcagcacgt
      541 tgtagatcgt cttggcgaag aagaagcagc cgacaaaagc gatcgcgaac gcgatcagcg
      601 ccttgatcag ccgcgagcgc agctcgatca agtgctccat cagcggcgct ttggtcgcct
      661 cgatctcctt ttcctcgtcc tcgcggctca tgcgggctta ccatcggacc tgactggatc
      721 agagccggcg gccgccggct cggcctccgc cgcggacggc gcggcgacat cgccgaccgg
      781 cgcgaccccc gcctcgccgc cgaggggcgc ggcattggat tgcgccacga cgggcgggct
      841 cgccgggctc gccgtctccg ccatcggctt ctcggccatc gcgctctcga tctgctgctg
      901 ggtgctttcc agctcccggc ggacctcgcc gacgggatcg aaattcgcat agctctgcgc
      961 ttggctcgtc atgtcgtcga cctgcttctt gaggtcggcc aactccgcct cgcgcatcgc
     1021 ttcctggaat tggccttgaa actcggaggc catgcggcgc agcttactca tccactgtcc
     1081 gagcgtgcgc agcacccccg gcagctcctt cggcccgatc gcgatcagcg cgacgatgcc
     1141 gatgatcagt agctcgcccc aaccgatttc gaacatgcgg gcttaaactc cgagaggcgc
     1201 gcgacgcccc ggcgcctctc cgatccaatc cgccggcttg tggccgcggc gaacgctcag
     1261 ccggctttgc gcgtctccgg ttgcgtgacg gtcgccgtgg gagcgggcgc gggctgatga
     1321 tcgatggtct tggggtcggt cttctccggc tcggtttcgg ctttgtcttc ctccgtcagg
     1381 cccttcttga aggacttgat gcccttggca aaatcaccca tcagctcgga gatcttgccg
     1441 cgtccgccga acagcagaag ggccacgccc agaaccagaa tccagtgcca aatgctcaat
     1501 gaacccatgc gcacatcctc cgcatcgacg agactgcacg ccgtctcgtc tctcggcttt
     1561 gcccaggaaa ctaggctccg cgtcgaccaa aaacaaggac tccgcccctt caccttgcct
     1621 cggcgacgcg tggcagcggt tgcggcgcgc cggtgcgttg gaaccgcacg cgttcagcgg
     1681 cctttcgccg cattaaccac agcagggctt cgagcggact tgaggcggcc cgccgcaaac
     1741 ccaggcatga tgggacgagg agcgcaacct cgcgattact cttcgccggt gcgctccggc
     1801 ggcggggcaa ggcccagatc gaggtcgccg ggttccaggg gatcttcgtc ctcgcgcaaa
     1861 gccgggtcat cggacggcat cggcacggag aagctagggg gcagccggcc gtcaagcagg
     1921 cccgagcctt tgagctcgtc gagacccggt aagtcgttca ccgcgtcgag cccgaagtgc
     1981 gacaggaacg cctcgctggt gccgtaggtc accggccggc cgggagactt gcgccggccg
     2041 cgcggacgga tccagccggt ctcgagcagg acatcgacgg agcccttgga cgtcgtcacc
     2101 ccacggatct cctcgatttc cgcccgcgtc accggctggt gataggcgat gatggccagc
     2161 gtctcgatgg ccgcgcgcga gagcttgcgt gtctcgacgg tttccttgct cagcaaccag
     2221 gagagatcgc cggcggtgcg aaacgtccac ttgccgccga tgcggacgag atggacccca
     2281 cgcgtcgcat aatcctcctg caatcgcagg agcgccgcgt gaacgtcgac cccttgcggc
     2341 agccgttccg ccaatgactt ctcgtcgagc ggctcggagg aggcgaacag catcgcttcc
     2401 aatagccgaa gctcctccgc tctcacctcg cccgcatgat ccggctcgtc gtccggcacg
     2461 accactttca acctcggcat tgctggcctc ctctaaccct tgccgtctcc cgatggcgcc
     2521 gccacgccac cggtatcggc caccccattt ccagattgtt ggcgtttgcg cacatagagg
     2581 ggcgcaaacg cgccctgctg gtgcacctcc atcaccccct cgcgcaccat ttcgagcgtg
     2641 gaggcgaagc tggacgcaag caccgtcgcc gccatcgacg gctcgaccac gtacgcgatg
     2701 agaaattcgt cgagccgcgc ccagtcggag gactggccga tcagccgctc gagcgcttct
     2761 cgcgcctcgg cgagcgacca gacggtgcgc ttggcgagcc gcacatgcgc gagcgccgtc
     2821 ttttgccgct gctgcgcgta ggccgaaagc aagtcgtaga gtgttgcgct gtattgcggg
     2881 tgcttgatgt gtgcaatcgg ctcggggtgg ccgcggcgga acacgtcgcg atcgagttgg
     2941 gagcggccga atagtcgctc ggcaacatcg cggatcgctt cgagccgctt gagccgcagc
     3001 gccagcgcga tcgccatgtc ctcggcgctc tggccttcg
//
LOCUS       DATNAE010000276         6076 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3878661, whole genome shotgun sequence.
ACCESSION   DATNAE010000276 DATNAE010000000
VERSION     DATNAE010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6076
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3878661"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="VN518_06210"
     CDS             complement(<1..359)
                     /locus_tag="VN518_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HXE04201.1"
                     /translation="MEYLAGKLKDADHAELLDALRAVMEEAPLFTPTMPRSGKPMSVR
                     MTNCGPLGWVTDKERGYRYQPTHPVTGKPWPAMPKPLLDLWREVAGYPADPEACLVNY
                     YSGGAKMGLHQDKDEED"
     gene            437..1267
                     /locus_tag="VN518_06215"
     CDS             437..1267
                     /locus_tag="VN518_06215"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXE04202.1"
                     /translation="MARGGKANPDGYVHTRDAKEYQRLRDQARMWEEATAHVLAAVGL
                     GPGMSCLDVGSGPGAVMRLMADRVGPQGRVTGIEIDGAFGAEALQDLRREGGAEFEQI
                     TADLLTLEAPAGVPFDLTFCRLLLMHMHDPVAILEKMLAWTKPGGVVVAQEFDFGAIA
                     VEPTCPAMGEFNRLFEGVFRAHGRNLRAGRQLPAQFEAAELGTPDGTDAAVHYLPLAQ
                     IATMLIGVYDGLYAAAAELGIADPLRASQFRKDMAEAGADGRYYCLTPVLIAAWKRIG
                     "
     gene            1484..3409
                     /gene="dnaK"
                     /locus_tag="VN518_06220"
     CDS             1484..3409
                     /gene="dnaK"
                     /locus_tag="VN518_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012169221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HXE04203.1"
                     /translation="MAKVIGIDLGTTNSCVAVMEGKTPKVIENAEGMRTTPSMVAFTD
                     DQERLVGLPAKRQAVTNPENTFFAIKRLIGRRYDDPMAEKDKGLVPYKIVRADNGDAW
                     VESRGKKYSPSEISAFILQKMKETAEAHLGEKVEQAVITVPAYFNDAQRQATKDAGKI
                     AGLEVLRIINEPTAAALAYGLDKKTGSKTIAVYDLGGGTFDISILEIGDGVFEVKSTN
                     GDTFLGGEDFDMRLVDYLANEFKKESGIDLRSDKLALQRLKEAAEKAKIELSSTTQTE
                     INLPFITADKSGPKHLTMKLTRAKLESLVDDLIQKTVGPCQNALKDAGLKAAEIDEVV
                     LVGGMTRMPKVVETVKTFFGREPHKGVNPDEVVAIGAAIQAGVLQGDVKDVLLLDVTP
                     LSLGIETLGGVFTRLIDRNTTIPTKKSQVFSTAEDNQNAVTIRVFQGEREMAADNKLL
                     GQFDLVGIPPAPRGVPQIEVTFDIDANGIVNVQATDKATGKEQSIRIQASGGLSDADI
                     DRMVKEAESHAEEDKKRRELVEMKNQGEALVHSTEKMLKDYGDKVPAADKGVVETALA
                     DLKSALQGEDKDAIKQKVDAMAQASMKLGEALYQANQGAAAAGGGGGPSEGGDGKEDV
                     VDVDFEEVKDDDSKKSA"
     gene            3538..4677
                     /gene="dnaJ"
                     /locus_tag="VN518_06225"
     CDS             3538..4677
                     /gene="dnaJ"
                     /locus_tag="VN518_06225"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HXE04204.1"
                     /translation="MTKRDYYDVLGIGRAASDQEIKAAFRRLAKECHPDTCNGDTTSE
                     KRFKEVNEAYEALKDPQKRAAYDRFGHAAFENGMGGMGGHPHGFGADFSASMSAMFDD
                     LFGEFMGQRRPRQRSARERGADLRYNMEITLAEAYEGKTAQIRVPTSVTCEACTGSGA
                     KTGTAPTTCRTCAGIGKVRAAQGFFTIERTCPSCHGRGEVIETPCPACNGSGRVTRER
                     TLSVTIPAGVEDGTRIRLAGEGEAGLRSGPAGDLYIFLSIKPHEFFQRDGADLFCRVP
                     IAMTTAALGGQVEVPTIDGGKSKVKIPEGTESGKQFRIKSKGMPVLRSKQQGDLYIQV
                     EVETPKSLTRKQRELLKEFENASNESTSPASTGFFARVKEFFEGQ"
     gene            4816..5391
                     /locus_tag="VN518_06230"
     CDS             4816..5391
                     /locus_tag="VN518_06230"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HXE04205.1"
                     /translation="MRLLFFAGSTREGSYNKKLARLAQHIATANGIEGVFVDLRDYPM
                     PLYHGDLEANEGPPQKAAEFKALLSEYQGVFIATPEYNSSTPALLKNTLDWVTRVRAK
                     GETGLEVYKTRVFAISGASPGYYGAMRSLLTLRQILVVGVGATVIPQQLALPRANDAF
                     EEDGSLKDKAQQNMLKDVVEKLAIAAKRFAA"
     gene            5391..>6076
                     /gene="pyrF"
                     /locus_tag="VN518_06235"
     CDS             5391..>6076
                     /gene="pyrF"
                     /locus_tag="VN518_06235"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HXE04206.1"
                     /translation="MALARDRLIVALDVPSADQARALIDALQDSVGVYKIGLELLFAG
                     GMALASELASRGMCVFIDAKLLDIEATVERATASIARTGAEFLTVHAVDGKTLDAAVR
                     GRGDSSLKLLGVTVLTNLGPADLVQQGTDIPPGELVLHRAMLAKEAGFSGVVASGLEA
                     AAIRDAAGTGFFIVTPGIRPQGGARQDQARVATPASAIAAGADYLVVGRPITAAPDPR
                     AAAEAIVAEI"
BASE COUNT         1203 a         1923 c         1976 g          974 t
ORIGIN      
        1 aagtcttctt cgtccttgtc ctgatgcagc cccatcttgg cgccgccgct gtagtaattg
       61 acgaggcacg cctccgggtc ggcaggatag cccgcgacct cccgccagag atcgagcagc
      121 ggcttcggca tcgccggcca gggcttgccc gtcaccggat gcgtcggctg atagcgataa
      181 ccgcgctcct tgtcggtgac ccagccgagc ggcccgcaat tcgtcatgcg caccgacatt
      241 ggcttgccgg aacgcggcat tgtgggggtg aacagcggcg cctcctccat cacggcgcgc
      301 agagcgtcca gcagctccgc atggtcagcg tccttcagtt ttcccgccag atattccaag
      361 gccacgcctt tgcctcaact tcgagccgtc ctacgatccg ccatagtacg cgtttaccgg
      421 tattctcgag gagatcatgg caagaggcgg caaagccaat cccgacggct acgtacatac
      481 gcgcgacgcc aaagaatatc agcgtctgcg cgatcaagcc cgcatgtggg aagaggccac
      541 ggcgcatgtg cttgccgccg ttgggcttgg cccaggcatg agctgcctcg acgtcggctc
      601 aggtccaggc gcggtaatgc ggctgatggc cgatcgcgtc ggaccgcaag gccgggtcac
      661 cggtatcgag attgacggcg cgttcggtgc cgaggcgctg caagacctcc gccgcgaagg
      721 tggcgccgaa ttcgagcaga tcaccgccga tcttctcacg ctcgaagcgc cggccggcgt
      781 gccgttcgac ctcaccttct gccggctcct gctcatgcat atgcatgatc ccgtcgccat
      841 ccttgaaaaa atgttggcat ggaccaagcc gggaggcgtc gtggtggcgc aggagttcga
      901 tttcggtgcc atcgccgtcg agccgacctg cccggccatg ggcgagttca accgcctgtt
      961 tgagggagtc ttcagagccc atggccggaa cttgcgcgcc ggacgccaat tgccggcaca
     1021 attcgaggcg gcggagctcg gcacgccgga cggcaccgac gccgcggttc actatctacc
     1081 gctcgcgcag atcgcaacca tgctgatcgg ggtttatgac ggtctttatg ccgcggccgc
     1141 cgagctcggc atcgccgatc cgttaagggc gtcgcagttc cgcaaggaca tggcggaggc
     1201 cggagccgat gggcggtact attgtcttac gccggttttg atcgccgcct ggaagcgtat
     1261 tggatgagaa acgcaagttg atcggggccc aagcaagcgc ttctccttgc ggcccaaatg
     1321 cttggctctt atataaccgc ggggaacccc gccggatgag tcggcgggcg cgccgatagt
     1381 attaggggac cggcgccacg cgatttcgca tgcgaggcgc gcgtcagcgt taacgggtgc
     1441 cgagtgaagg gcccggacgg tctgccagaa agagaggcta cggatggcga aagtaattgg
     1501 tatcgatctc ggcacgacga attcctgtgt cgcggtcatg gaggggaaga cccccaaagt
     1561 tatcgagaat gccgaaggca tgcgcaccac gccttcgatg gttgccttca ccgacgatca
     1621 agagcgactg gtcggacttc ccgccaagcg ccaagcggtc accaatcccg aaaatacctt
     1681 cttcgccatc aagcggctga tcggccgccg ctacgacgac ccgatggccg agaaggacaa
     1741 gggcctcgtt ccctacaaga ttgtccgcgc cgataacggc gacgcctggg tggaatcccg
     1801 cggcaagaaa tactcgccat cggaaatctc ggccttcatc ctacagaaga tgaaagagac
     1861 ggcggaagcc catctcggcg agaaggtcga gcaggcggtg attaccgtgc cggcctattt
     1921 caacgacgct cagcgtcagg cgaccaagga cgcgggcaag atcgccggcc ttgaagtgtt
     1981 gcgcatcatc aacgagccga cggctgccgc gctcgcttac ggtctcgaca agaagaccgg
     2041 ctcaaagacc atcgccgtct acgaccttgg cggcggcacc ttcgacattt ccatcctcga
     2101 gatcggcgac ggcgtgttcg aggtgaagtc gaccaatggc gataccttcc tcggcggcga
     2161 ggatttcgac atgcgcctcg tcgactatct cgccaacgag ttcaagaagg agagcggcat
     2221 cgatctcagg agcgataagc tcgcgctgca gcgtctgaag gaagccgccg agaaggccaa
     2281 gatcgagctg tcgtcgacga cgcagaccga gatcaacctg cccttcatca ccgccgacaa
     2341 gagcgggccc aagcatctca ccatgaagct caccagggcc aagcttgaga gcctggtgga
     2401 cgatctcatt cagaagacgg tcgggccttg ccagaacgcg ttgaaggacg ccggcctcaa
     2461 ggccgccgag atcgacgagg tggttctcgt cggcggcatg acgcgcatgc cgaaagtggt
     2521 cgagacggtg aagaccttct tcggccgcga gccgcataag ggcgtgaacc ctgacgaggt
     2581 ggtggccatc ggtgccgcaa tccaggccgg cgtgctgcag ggggacgtca aggacgtgct
     2641 cctgctcgac gtcaccccac tgtcgctcgg catcgagacc ctgggcgggg tgttcacgcg
     2701 gctcatcgac cgcaacacca cgatcccgac caagaagagc caggtgttct cgaccgccga
     2761 ggacaatcag aacgcggtca ccatccgcgt cttccaaggc gagcgcgaga tggctgccga
     2821 caacaagctg ctcggccagt tcgatctcgt cggcattccg ccggcgccac gcggcgtgcc
     2881 gcagatcgag gtcaccttcg acatcgacgc caacggcatc gtcaatgtgc aggcaaccga
     2941 taaggccacc ggcaaggagc agtcgatccg catccaggcg tcaggtggtc tttccgatgc
     3001 cgacatcgac cgcatggtca aggaagccga gagccacgcc gaggaggaca agaagcgtcg
     3061 cgagctggtc gagatgaaga accagggcga ggcgctggtg cactccacgg agaagatgct
     3121 caaggactat ggcgacaagg tcccggcggc cgacaagggc gttgtggaaa ccgcgcttgc
     3181 cgacctcaag tcagccctgc aaggcgagga caaggacgcg atcaagcaaa aggtcgacgc
     3241 catggcgcag gcctcgatga agctcggcga agcgctttat caggcaaacc agggcgcagc
     3301 cgctgcgggc ggcggtggag gcccctcgga aggcggcgac ggcaaagagg atgtggtcga
     3361 cgtcgacttc gaggaggtca aggacgacga cagcaagaag tcagcataag tagcatcgta
     3421 ggtgttggcg tgaggcttgc acgtgtccgt gcagcctcgg gccgcaagcc caagacaaga
     3481 ggctaagcgc cgccgccagc gggtgggcgg gttggtgtgg aaggccggat agcgcgcatg
     3541 acgaagcgag attattacga cgtcttgggt atagggagag cggcttccga tcaggagatc
     3601 aaggccgcct ttcgccgtct cgccaaggaa tgccaccccg acacttgcaa cggcgacacc
     3661 acctcggaga agcgcttcaa ggaagtcaac gaagcctatg aggcgttgaa agacccgcag
     3721 aagcgggcgg cctatgaccg cttcggccac gcagccttcg agaacggcat gggaggcatg
     3781 ggcggccacc cgcacgggtt cggtgccgac ttctcagcct cgatgtcggc catgttcgac
     3841 gatctgttcg gcgagttcat gggccagagg cggccgcgtc agcgctcggc gcgcgaacgc
     3901 ggcgccgatc ttcgctacaa catggagatc acgctcgccg aagcctatga aggcaagacg
     3961 gcgcagatcc gcgtgccgac atccgtcacc tgcgaggcct gcaccggctc aggcgccaaa
     4021 acgggcaccg cgccaacgac ctgccgcacc tgcgccggca tcggcaaggt gcgcgcggcg
     4081 caaggcttct tcaccatcga gcgcacctgt ccctcctgcc atggacgcgg cgaggtgatc
     4141 gaaaccccgt gcccggcctg taatgggtca ggccgggtca cgcgcgagcg cacgctgtca
     4201 gtgaccatcc cggcaggcgt ggaagacggc acgcggatca ggctcgccgg cgaaggcgag
     4261 gcaggattgc gcagcggacc cgccggcgac ctctacatct tcctctccat caagccgcat
     4321 gagttcttcc agcgcgacgg cgccgacctg ttctgccgcg tgccgatcgc catgaccacg
     4381 gccgcgcttg gcggtcaggt cgaggtgccg accatcgacg gcggcaagtc gaaggtgaag
     4441 attcctgagg gcaccgagag cggcaagcag ttccgcatca agtcaaaggg catgccggtg
     4501 ctgcggtcga agcagcaggg cgacctctac atccaggtcg aggtcgagac gccgaagagc
     4561 ctcacccgta agcagcggga gctgttgaag gagttcgaga acgcctcgaa cgagagcacg
     4621 agcccggcgt cgaccggttt cttcgcccgc gtgaaggaat tcttcgaggg ccaatagcgg
     4681 ccccgcgtct atccgcatat ctccgcatat atccgcacaa ttccgcgcat ttttccgcgc
     4741 atatcggcgc atatcccgcg ggaaatcgcg tagagtgcga cgtctggcgc gggagggcta
     4801 cgggaggagt gacgaatgcg gctcttgttc ttcgccggca gcacacggga gggctcctac
     4861 aacaagaagc ttgccaggct cgcccagcac atcgccacgg cgaacggcat cgaaggggtg
     4921 ttcgtcgacc tcagggacta tcccatgccg ctctatcacg gcgacctcga ggccaacgaa
     4981 ggaccgccgc agaaggccgc agagttcaag gcgctgctca gcgagtatca gggcgtgttc
     5041 atcgcgactc ctgagtataa ttcctcgacc ccggcgctcc tcaagaacac gctcgactgg
     5101 gtgacgcggg tcagggccaa gggcgagacc gggctcgaag tctacaagac gcgggtgttc
     5161 gccatcagtg gggcctcgcc aggctattac ggcgccatgc gcagcctgct cactctcagg
     5221 cagattctcg tcgtcggcgt cggcgccacg gtgatcccgc aacagctcgc cctgccccgc
     5281 gccaacgacg ccttcgagga ggacggcagc ctcaaggaca aggcgcaaca aaacatgttg
     5341 aaggacgtgg tggagaagct cgccatcgcc gccaagaggt tcgccgcgtg atggccctcg
     5401 cacgcgaccg gctgatcgta gccctcgacg tgccaagcgc ggaccaggcg cgggcgttga
     5461 tcgacgcgct gcaagacagc gtcggggtct acaagatcgg ccttgagctt ctgttcgcag
     5521 gcggcatggc gctcgcaagc gagcttgcct cgcggggcat gtgcgtgttc atcgacgcca
     5581 agctgctcga tatcgaggcg acggtcgagc gtgccacggc ttcgatcgcc cgcaccggcg
     5641 ctgagtttct caccgtgcac gcggtggacg ggaagacgct cgacgccgcg gtgcgaggcc
     5701 ggggcgacag cagcctcaag cttctcggcg ttacggtgct gaccaatctc gggccggcgg
     5761 acctcgtgca gcaaggcacc gacattccgc ctggcgagct cgtgctgcac cgagccatgc
     5821 tggccaaaga ggctggcttc agcggcgtcg tcgcctccgg gctcgaggcc gcggcgatcc
     5881 gcgatgccgc gggcacaggg tttttcatcg tcacgccggg catcaggcct caaggcggcg
     5941 caaggcagga ccaggcgcgg gtcgcgacgc cggcaagcgc cattgccgcc ggggccgatt
     6001 atctcgtggt cggccggccg atcaccgcgg cacctgaccc tcgcgccgcg gccgaggcga
     6061 tcgtcgcaga gatcga
//
LOCUS       DATNAE010000277         2222 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_4486209, whole genome shotgun sequence.
ACCESSION   DATNAE010000277 DATNAE010000000
VERSION     DATNAE010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4486209"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1351
                     /locus_tag="VN518_06240"
     CDS             <1..1351
                     /locus_tag="VN518_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014130219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXE04207.1"
                     /translation="LWLEKYLATYPYTVFMISHDRDLLNKAVSSIIHLERGKLTLYRG
                     AYDTFEETRRMQMELNNKAREKTLEQIAHMQKFVDRFKAKATKAKQAQARVKAIAKLK
                     PPEALYDEYSAPFSFPQPKSQMASPMVTMENVSTGYGETVVLKRLSLRLDPDDRIALI
                     GVNGNGKSTFAKLLAGDLKPFGGEMLRGKKLEVAYFAQHQMDKLKPALTPLEHMPELM
                     PYDNETKRRSRLAQMGLSASRMDTKVEKLSGGERARLLLGLVTFGGPGLLILDEPTNH
                     LDIDSRDALVYALNEYRGAVLIISHDRHLIDSTCDRIWIAEGGTVHVFDDDLDAYQRM
                     IVSGGNKGAASSTSTAPVAAADDRKTQRQEAAAKRAEIAPLRKSIRDAEQKIDRLKNE
                     LAKVEVILADPKLYDGAPERIVKLGKDKARFEGEIARVEDNWLSLNAELEAAERAQA"
     gene            1348..1779
                     /locus_tag="VN518_06245"
     CDS             1348..1779
                     /locus_tag="VN518_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXE04208.1"
                     /translation="MSELAASEVIALLELERHPEGGWYRQTFADPHPQGQRHLSTAIY
                     YLLEGNDVSAWHRVDAAEIWHWHAGAPLRLRLSHEGKVIDEHTLGPDLKAGERPQVIV
                     PRQCWQTAKSLGDWTLVGCTVAPGFEFSGFEMAETGWEPGR"
     gene            complement(1776..>2222)
                     /locus_tag="VN518_06250"
     CDS             complement(1776..>2222)
                     /locus_tag="VN518_06250"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HXE04209.1"
                     /translation="GSFLMVGIPLLPVGHIPNAIKVIGELLMIMGIVGSIFAMSWLGR
                     SYSIMATARNLVTAGPYRIVRHPLYAAECLALVGIVITCFSPFALAIGLMTLGLWWRR
                     AINEERILTQAFPEYAEYARQVPRFVPRVLSGGMLRPAAAGAVFGT"
BASE COUNT          454 a          712 c          731 g          325 t
ORIGIN      
        1 gctctggctc gaaaagtacc tcgcgaccta tccctatacc gtcttcatga tcagccacga
       61 ccgcgacctg ctcaacaagg cggtctcctc gatcatccat ctcgagcgcg gcaagctgac
      121 gctctatcgc ggcgcctacg acacgttcga ggagacgcgg cggatgcaga tggagctcaa
      181 caacaaggcg cgcgaaaaga cgctcgagca gatcgcgcac atgcagaaat tcgtcgatcg
      241 cttcaaggcg aaggcgacca aggccaagca ggcgcaggcc cgcgtcaagg cgatcgccaa
      301 gctcaagccg cccgaagcgc tctacgacga atactcggcg ccgttctcgt tcccgcagcc
      361 gaaatcgcag atggcgtcgc cgatggtgac catggagaac gtttcgaccg gctatggcga
      421 gaccgtggtg ctgaagcggc tcagcctcag gctcgatccc gacgaccgca tcgccctcat
      481 cggcgtcaac ggcaatggca agtcgacctt cgccaagctc ctcgccgggg atttgaagcc
      541 gttcggcggc gagatgctgc gcggcaagaa gctcgaggtc gcctatttcg cccagcatca
      601 gatggataag ctcaagccgg cgctgacgcc gctcgagcac atgcccgagt tgatgcccta
      661 cgacaacgag accaagcgcc ggtcgcggct ggcgcagatg gggctgtccg catcgcgcat
      721 ggacaccaag gtcgaaaagc tctcgggcgg cgagcgggcg cgcctcctgc tcgggctcgt
      781 gaccttcggc gggccgggtc tgctgatcct cgacgagccg accaaccacc tcgacatcga
      841 tagccgcgat gcgctggtct atgcgctcaa cgagtatcgg ggggcggtgc tgatcattag
      901 ccatgaccgc catctgatcg attccacctg cgaccggatc tggatcgcag agggtggcac
      961 ggtgcacgtc ttcgacgacg acctcgacgc ctatcagcgg atgatcgtca gcggcggaaa
     1021 caagggcgcc gcttcgtcga cctccactgc ccctgtggcc gcggcggatg accgcaagac
     1081 tcagcggcag gaagcggcgg ccaagcgcgc cgaaattgcc cccttgcgca agtcgatccg
     1141 cgacgccgag cagaagatcg accggctgaa gaatgagctc gccaaggtcg aggtgatcct
     1201 cgccgacccc aagctctacg acggcgcgcc cgagcggatc gtcaagctcg gcaaggacaa
     1261 ggcgcggttc gagggcgaga tcgccagggt cgaggacaac tggctcagcc tcaatgccga
     1321 gctcgaagcg gcggagcggg cgcaggcatg agcgagctag ccgccagcga ggtgattgcc
     1381 ttgctcgagc tcgagcggca tccggagggc ggctggtatc ggcagacctt cgccgatccc
     1441 cacccgcaag ggcagcggca cctgtcgacg gcgatctact acctgctcga agggaacgac
     1501 gtgtcggcct ggcatcgggt ggatgcggcc gaaatctggc actggcatgc cggcgcgccg
     1561 ctgcgcctgc gtctcagcca cgaaggcaag gtgatcgacg agcacacact gggccccgac
     1621 ctcaaggccg gcgaacggcc gcaggtgatc gtgccgcgtc agtgctggca aacggcgaaa
     1681 agccttggcg actggacgct ggtcggctgt acggtggcgc ccggcttcga gttctcgggc
     1741 ttcgagatgg ccgaaacggg ctgggagccg ggacgctagg tgccgaaaac ggctcccgct
     1801 gcggccggcc gcagcatgcc accgctcagc acgcgcggaa caaagcgcgg cacctgacgg
     1861 gcatattccg catattccgg aaaggcctgg gtcaggatgc gctcctcgtt gatggcgcgc
     1921 cgccaccaaa ggccgagcgt catcaggccg atggccaagg caaacggcga aaagcaggtg
     1981 atgacgatgc cgaccaaagc caggcattcc gcggcgtaga gcggatgccg cacgatgcgg
     2041 tagggaccgg cggtgacgag gttacgggcc gtcgccatga tcgaatagga gcggccgagc
     2101 caggacatcg cgaagatcga ccccacgatc cccatgatca tcaggagttc gccgatgacc
     2161 ttgattgcgt tggggatatg cccaacgggc agcaacggaa tgccgaccat caggaaggag
     2221 cc
//
LOCUS       DATNAE010000278         3328 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_4489794, whole genome shotgun sequence.
ACCESSION   DATNAE010000278 DATNAE010000000
VERSION     DATNAE010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4489794"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VN518_06255"
     CDS             <1..198
                     /locus_tag="VN518_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HXE04210.1"
                     /translation="DAKAVLDGAAGERAASVYAGNTEAAIRADAIGVPTYVLNGEAFW
                     GQDRLDLLDRALTSGRKPYLP"
     gene            292..942
                     /locus_tag="VN518_06260"
     CDS             292..942
                     /locus_tag="VN518_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013897283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXE04211.1"
                     /translation="MLSHERVWAAIDALAERYSLSASGLARRAGLDSTAFNKSKRMSA
                     DGRPRWPSTESLAKIIDATGSSLDELFALIEQKAGLPGGALPRQAAPVPLVGFAQAGA
                     GGFFDDSGFPAGEGWDLIELPGRAPEGTYALGIQGDSMLPLYRHGDILIVDPNAPVRR
                     GDRVVVKTTGGEVMAKVLERQTTKAIDLLSLNPEHENRTIPAAQVDWVARVIWASQ"
     gene            953..1678
                     /locus_tag="VN518_06265"
     CDS             953..1678
                     /locus_tag="VN518_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HXE04212.1"
                     /translation="MPTLRRIALFLAGVALVGTLWIGGRSVVEPPDKGTEPGRQTRDE
                     RPPDPRQSIKPSIETGQKAAGENPQVRRIAPGSIAGPEISGPLERITPREPLAAATKP
                     LKREPFGPPIPQGTLLYQPVASAAGEIEANGHEVRLAGIKVTPANRTCKDAGGRVWPC
                     GVVARTAFRAWMRGRAVACDVPSHAGAITTHCTLDGADMALWLVRNGWAESADGQYDD
                     AEAKARRDKMGMFGPSPLGAGKE"
     gene            complement(1905..3182)
                     /locus_tag="VN518_06270"
     CDS             complement(1905..3182)
                     /locus_tag="VN518_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018426361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXE04213.1"
                     /translation="MVFDILVRQGILPDGVVADIGISGEKIAAIGPKLEAEAGRIVEA
                     AGNLLSPPLVDPHFHMDATLSYGIPRINASGTLLEGIALWGELKPMLTHEAVKERALA
                     YCDWAVSMGLLAIRTHVDVCDDRLLAVEALLEVRKEVAPYIDLQLVAFPQDGFYRDPT
                     ARANTIRALDMGVEVVGGIPHFERTMADGTRSVTELCEIAAQRGLMVDMHCDETDDPL
                     SRHVEQLAYETQRLGLRGRVAGSHLTSMHSMDNYYVSKLLPLIAEAGMAAIPNPLINI
                     VLQGRHDTYPKRRGMTRVPEMLKAGIRVGFGQDCVLDPWYSLGTADMLDVAFMGLHVA
                     QMTSPADMRRCFDMVTTESAAIMGLEDYGLAVGKRASLVVLDAGNPVEAIRLRAERLC
                     VIARGKIVAERPRRETQLFLDGRPQSVHRRHVV"
BASE COUNT          596 a         1086 c         1097 g          549 t
ORIGIN      
        1 gacgccaagg ccgtgcttga tggggccgcc ggcgagcgcg cggcgtccgt ctatgcggga
       61 aacactgagg ccgcgatccg cgccgacgcc atcggcgtgc ccacctatgt gctcaacggc
      121 gaggcgttct ggggccagga ccggctcgat ctcctcgacc gcgcgctgac gagcgggcgc
      181 aagccttacc ttccctgata tttcacctgg gtcggcttct ggacaaaagc cgacgccgcc
      241 gatagaagca gggaaaggta ttttttcctg taccaggaag ggagcaccgc catgctctcg
      301 catgaacgtg tatgggcggc aatcgacgcg cttgccgagc gctattcgct ttccgcgtcc
      361 gggctcgccc ggcgcgccgg cctcgattcg accgccttca acaagtcgaa gcggatgtca
      421 gccgacggca ggccgcgctg gccttcgacg gaatcgctcg ccaagataat cgatgcgacg
      481 ggatcgtcgc ttgacgaact cttcgcgttg atcgagcaaa aggccggatt gccgggcggc
      541 gcgctgccga ggcaggcggc gccggtgccg ctggtcggct tcgcgcaggc cggagccggc
      601 ggcttcttcg acgattccgg ctttcccgcc ggcgaaggct gggaccttat tgaacttccg
      661 ggccgcgcgc ccgaaggcac ctacgcactg ggcatccaag gcgattccat gctgccgctc
      721 taccgccatg gcgacatcct gatcgtcgat cccaacgctc cggtgcgccg gggcgaccgc
      781 gtcgtggtca agacgaccgg cggcgaggtg atggcgaagg tgctcgaaag gcagacgacc
      841 aaggccatcg acctcctctc gctcaatccg gagcacgaga accgcacgat cccggcggcg
      901 caagtcgatt gggtggcgcg ggtcatctgg gcaagccagt aagcttcgtc cgttgcccac
      961 gctcaggcgc atcgcgctct ttctcgccgg agtggcgctc gtcggcacgc tctggatcgg
     1021 cggccgctcc gtggtcgagc ccccggacaa aggcacggaa cccggccgtc agacccggga
     1081 tgagcgacct ccagatccga ggcagtccat caagccttcg attgagaccg gccaaaaggc
     1141 tgccggagag aatccgcagg tccgccggat cgcgcccggc agcatcgccg gaccggaaat
     1201 ctccggacct ctggagcgta tcacgccgcg ggaaccgctc gccgcggcca ccaagccgct
     1261 gaagcgggag ccattcgggc cgcccatccc gcaaggcacg ttgctctacc aaccggtcgc
     1321 ctccgccgcc ggcgaaatcg aggccaacgg ccatgaggtg cggctggcgg ggataaaggt
     1381 gacgccagca aaccgcacct gcaaggatgc gggcggacgc gtctggcctt gcggcgtcgt
     1441 ggcgcgtacc gcgttccggg cgtggatgcg tggccgcgcc gtcgcctgcg acgtgccatc
     1501 gcatgcggga gccatcacca cccattgtac gctcgacggc gctgacatgg cgctatggct
     1561 ggttcgcaac ggctgggcag agagcgccga cggccagtat gacgacgccg aggcgaaggc
     1621 ccggcgggat aagatgggca tgtttggccc ttcgccgctg ggagcgggga aggaataaga
     1681 aagcgatgtc gcttcgatca tcggtgctcg ccggcgccgg ccattcagct accttcgctt
     1741 tgaattgagg acgattgcaa acgaaactcg ctcccctctc cggtttgctt cgcaaaccac
     1801 ctctccccct gaagggggcg aggtgtcgag gcgatagccg agacggagag ggggaagttt
     1861 ccgcagcttt catattcgat agccatcact cttgacggaa gattttatac cacatgccgg
     1921 cggtgcaccg attgcgggcg gccgtcgagg aacagctggg tctcgcggcg cggccgctcg
     1981 gcgacgatct ttcccctggc gatgacgcag agccgctcgg cgcgcagcct gatcgcctcg
     2041 accgggttgc ctgcgtccag cacgacgaga ctggcgcgct tgccgacggc aagcccataa
     2101 tcctctagcc ccatgatggc cgcgctctcg gtggtcacca tgtcgaaaca gcggcgcatg
     2161 tcggcggggc tcgtcatctg cgccacatgc aaacccatga aggcgacgtc gagcatgtcg
     2221 gcggtgccga gcgaatacca ggggtcgagc acgcaatcct ggccgaagcc gacgcgaatg
     2281 ccggccttca gcatctccgg cacgcgggtc atgccgcgcc gcttgggata ggtgtcgtgg
     2341 cggccctgca gcacgatgtt gatgagcgga ttgggaatcg ccgccatgcc ggcttcggcg
     2401 atcagcggca ggagcttgga gacatagtaa ttgtccatcg aatgcatcga ggtgaggtgc
     2461 gatccggcca ccctgccgcg caagccgagg cgctgcgtct cataggcaag ctgctcgaca
     2521 tggcgcgaca gcgggtcgtc ggtctcgtcg caatgcatgt cgaccatcag gccgcgctgc
     2581 gccgcgatct cgcagagctc ggtcaccgag cgcgtgccgt cggccatggt gcgctcgaaa
     2641 tgcgggatgc cgccgacgac ctcgacaccc atgtcgagcg cgcggatcgt gttcgcgcgc
     2701 gccgtcgggt cgcgatagaa gccgtcctgc gggaaggcga cgagctgcag gtcgatatag
     2761 ggcgcgacct ccttcctcac ctcgagcagg gcctcgaccg ccagcaggcg gtcgtcgcag
     2821 acatcgacat gggtacggat ggcgagcagg cccattgata cggcccagtc gcaataggcg
     2881 agcgccctct ccttcaccgc ctcatgcgta agcatcggct tcagctcgcc ccagagcgca
     2941 atgccttcga gcagcgtgcc cgacgcgttg atgcgcggga tgccgtagga aagcgtggcg
     3001 tccatatgga aatgcgggtc gacgagaggc ggcgaaagga ggttgcccgc cgcctcgacg
     3061 atccggccgg cctcggcttc gagcttcggt ccgatggcag caattttttc acccgaaatg
     3121 cctatatcgg caaccacgcc atcaggcaaa atgccctgtc tgaccagaat gtcgaatacc
     3181 atcttatgcg ggaacctgtt ggagaggaga agcactgtag cgaggcgtta gacgaactga
     3241 caagcgctcg atttccggcc ttcagaatac cccctcaccg cctcgcttcg ctcggcacct
     3301 ctccccgcta cgtggggacg aggagagg
//
LOCUS       DATNAE010000279         9966 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3283785, whole genome shotgun sequence.
ACCESSION   DATNAE010000279 DATNAE010000000
VERSION     DATNAE010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 9966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9966
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3283785"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /gene="efp"
                     /locus_tag="VN518_06275"
     CDS             <1..556
                     /gene="efp"
                     /locus_tag="VN518_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011582497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HXE04214.1"
                     /translation="NGNEIRPGNVITHKGGLWIAVKTQAVKPGKGPAYAQVELKNVID
                     GTKLNERFRSSETVERVRLEQNDYQFLYAQGDMLVFMDTDTYDQIELPAEFLEERAAF
                     LQEGMKVTVESHEGKPIGVAIPEQVTLAVVEADPVVKGQTAASSYKPAMLENGVRILV
                     PPFVVAGERVVVDTNELTYVRRAE"
     gene            694..1524
                     /locus_tag="VN518_06280"
     CDS             694..1524
                     /locus_tag="VN518_06280"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008191746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HXE04215.1"
                     /translation="MPASALMNVIVGAARKAGRSLARDFGEVEQLQVSMKGPANFVSA
                     ADTRAEEILHKELLRARPGYGLLLEEHGEVEGADRTHRFIVDPLDGTTNFLHGIPLFA
                     IAIALERDGELVAGLIYNPASNEMFTAERGKGAFLNDRRIRVAARTELADCVIVTGIP
                     HRGKPGQDLFLREMEAVMSVTAGVRRTGAAALDLAWVAAGRFDGFWERNLRAWDLAAG
                     IVILREAGGFVSDADGKDKMLETGSVVAGNESIQRKLLKQLKAAQVPRDAVAAADPSR
                     "
     gene            1649..2683
                     /locus_tag="VN518_06285"
     CDS             1649..2683
                     /locus_tag="VN518_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotA"
                     /protein_id="HXE04216.1"
                     /translation="MAATSTYKAKLTYPQIFLWRMSLFLILAILLAVILDGQTQQLRT
                     SFLANPGLNSLILGVLFVGVVYAFRQVLRLYPEIRWVNSVRISDPGLAVDTTPMLLAP
                     MATMLRDRTGHLSLATGAMRSLLDSVASRLDEQRETTRYLVGLLVFLGLLGTFWGLLQ
                     TVTSVGSTISALDTNGSESTMLFDQLKEGLAAPLKGMGTAFSCSMFGLGGSLILGFLD
                     LQAGHAQSRFYNELEDWLSGITELQSAEGTTVAGVPPQLRFALLDMQRSLSDLSEKIE
                     LRVLGDNTGDAVKDLAQGVEKLIRQMRAEQKVVREWADEQAQHQHELAVALKQITGKV
                     KLPVHEEERS"
     gene            2686..3711
                     /locus_tag="VN518_06290"
     CDS             2686..3711
                     /locus_tag="VN518_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan -binding protein"
                     /protein_id="HXE04217.1"
                     /translation="MALAKSRRGPAVNYWPGYVDMLSTLLLVVTFLMSLFMLSNYFIT
                     TQTSGKDTMLSRLNRQLSQLTELLALERSKKESIEDSLSALQATLIDKDKENARLTGL
                     LGDQSGKTEGAEAKTSALSGQLDQQKQITNEALAKVEILNQQLAALRLQLAALQEALD
                     ASEAKDRESQVKIADLGQRLNVALAKKVQELARYRSDFFGKLREVLGSRADFQVVGDR
                     FVFPADVLFDSGSADLKPEATPQIDKLADALKQLETQIPPDIAWVLRIDGHTDVKPIT
                     NVNFPSNWELSSARAISVVRYLIDHGIPANRLVAAGFGEFQPLDPGDSDEALAKNRRI
                     ELKLTER"
     gene            3766..4203
                     /locus_tag="VN518_06295"
     CDS             3766..4203
                     /locus_tag="VN518_06295"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HXE04218.1"
                     /translation="MLCYIIAMNAAHHHDHPKLSRNQGQVLSALRKAKEPLSAYAILD
                     RVRTSGISHPPTVYRALNELIGLGMVHRLQSRSAFVACDHGACNGKAAFAICRQCEKV
                     VEISLSDKDERVLLALSPKDIAAEQITLEIAGLCEACRPAKAT"
     gene            4249..4416
                     /locus_tag="VN518_06300"
     CDS             4249..4416
                     /locus_tag="VN518_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE04219.1"
                     /translation="MDQRSGIGHEHADEHALMPPRQGFSLLRLSVPARLAMVGAITAL
                     LWAFVWLWALR"
     gene            4413..5189
                     /gene="aztA"
                     /locus_tag="VN518_06305"
     CDS             4413..5189
                     /gene="aztA"
                     /locus_tag="VN518_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein AztA"
                     /protein_id="HXE04220.1"
                     /translation="MTQEKPPAISFHDLTLCYDRHPAVHHITGNVERGDLLALVGPNG
                     AGKSTLLKGIIGQLKPLAGSLSLDGLKRNDIAYLPQQIDIDRSFPITVFDAVSMGLWR
                     QIGTWRGLDRGLNLAVLEALATLGLQDLSKRSVGALSGGQFQRVLFARLLLQDAAIIL
                     LDEPFRAVDTKTVEDLLRLISRWHGEGRTVIAALHDLDQVREHFPKTLLLAREVVAWG
                     PTKQVLTAKNLVKSRQLTEAWDEHAEICERGEARTERISA"
     gene            5186..6052
                     /locus_tag="VN518_06310"
     CDS             5186..6052
                     /locus_tag="VN518_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010682292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HXE04221.1"
                     /translation="MIHALIDPFVEFAFMRRALVGCFALSLGAPPVGVFLVLRRLSLM
                     GDAMSHAILPGAAIGFLVAGLSVPAMSLGGFIAGIAVVLLAGLVTRSTILREDASLAA
                     FYLVSLALGVLIVSMKGSSIDLLHVLFGTVLALDDTALILIAAIATLTMATLAIIYRP
                     LVLECFDPSFLASVSPLSSPTHFLFLVLVVMNLVGGFQALGTLMVVGIMLLPAITARF
                     WAQDISNMIIIAVGAAFAASFIGLLLSYYLNLPTGPAIILTSGAGYLISMLIGTEGGL
                     LWQLVPRKHLEA"
     gene            6076..6972
                     /locus_tag="VN518_06315"
     CDS             6076..6972
                     /locus_tag="VN518_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HXE04222.1"
                     /translation="MHRRSFLIAAAALAAMLGATAGAQAAEPMKVSASFSILGDMVRQ
                     VGGDRVEVFTFVGPNGDAHVYEPTPADAKELSESKILFINGLGLEGWMTRLEKSSGFK
                     GTVVTASKGVAPREMVEDEKTVTDPHAWQSLANGKIYVANIRDALIAADPEGKATYEA
                     NAKEFLERIAAMETTVKEAIAKLPPERRKIITTHDAFGYFGAAYGMEFIAPEGVSTES
                     EASAKDVAKIIRQIKAEKVPAVFLENVTDSRLLEQIAKETGAKIGGTLFSDALSPPDG
                     PAGTYLDMFHNNIETMSEALSS"
     gene            7016..7924
                     /locus_tag="VN518_06320"
     CDS             7016..7924
                     /locus_tag="VN518_06320"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HXE04223.1"
                     /translation="MRRCVLLALAIGLVALGWSAASHAEDRKEREFQECPECPVMVGI
                     PAGTFVMGSPHTESGRFDSEGPQHEVAIKAFALGKYDVTSEQFLTFLKETGYQPRPCS
                     TILDMKWKSPGHGVAYSPLNGEPLHWPAVCLDWHDAEKYIAWLNDKVRIAHPTLDHAK
                     GAYRLPTEAEWEYAARAGTTTARSWGNDIGAGKANCNGCGSKWDNRLFADVDSFAPNP
                     FGLYGMLGNAWQWTADCWHPNYLGAPRDGNALVEADCDKHVIRGGSWSNLPNFVRSAA
                     RSGSGRNSGEYDYSSLTGFRVARDLP"
     gene            complement(7925..8686)
                     /locus_tag="VN518_06325"
     CDS             complement(7925..8686)
                     /locus_tag="VN518_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE04224.1"
                     /translation="MDLGDEEPLRVEPVGPIGPSETQGRESAAGLALSVMLHGIALCL
                     LLVVGVFGTEGSQGGLEIVPVEVEITNQSASAASEPPTAALPQPEAAHPSDATAHGAA
                     RASEPVADELKAKLEALAKLRQPEAVTPSEENGAARPDRVAKNDDIASGLEGPFSVKD
                     YIRAQVERRWNPDLANLRNHEVSVPIHVEITSAGVVLKAEIVDTARAADPDFREIAVS
                     ARNAVLLSSPIALPAGHYQEVMDMVLYLNPKDALR"
     gene            8798..>9966
                     /gene="ispG"
                     /locus_tag="VN518_06330"
                     /gene_synonym="gcpE"
     CDS             8798..>9966
                     /gene="ispG"
                     /locus_tag="VN518_06330"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020083985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HXE04225.1"
                     /translation="MESSARRRSVPVNIGGVIVGGTAPIVVQSMTNTDTADIDATVAQ
                     VAALARAGSELVRITVDRDEAAAAVPHIRERLDARGVDVPLIGDFHYNGHTLLAEYPA
                     CAQALAKYRINPGNVGFKDKRDRQFSAIVDIAIRNDKPVRIGVNWGSLDQDLLTRLMD
                     ENAKLPEPKDARAVMHEAIAQSALLSAERAVELGLGPDKIVLSAKVSAVPDLIQVYAM
                     LAARSDLALHLGLTEAGMGSKGIVASSAALAVLLQQGIGDTIRISLTPEPNGDRTLEV
                     KVAQELLQSMGFRSFVPLVAACPGCGRTTSTTFQELARDIQDFIRDEMPEWKRRYPGV
                     ETMTVAVMGCIVNGPGESKHADIGISLPGTGEAPAAPVFIDGRKVATLRGPGAAA"
BASE COUNT         1760 a         3415 c         3034 g         1757 t
ORIGIN      
        1 caatggcaac gagattcgcc caggcaacgt gattacgcac aaaggcgggc tgtggatcgc
       61 ggtcaagacg caggccgtga agccgggcaa gggtccggcc tatgcccaag tcgagctgaa
      121 aaacgtcatc gacggcacca agcttaacga gcgcttccgc tcttcggaga cggtcgagcg
      181 cgtgcggctc gagcagaacg actatcagtt cctctacgcc cagggcgaca tgctcgtgtt
      241 catggacact gatacctacg accaaatcga gcttccagcg gaattcctgg aggagcgcgc
      301 ggccttcctg caagagggca tgaaggtgac ggtggagagc catgaaggca agccgatcgg
      361 cgtcgccatc cccgagcagg tcacgctcgc tgtggtcgag gccgatcccg tcgtgaaggg
      421 ccagaccgcc gcctcctcct acaagccggc catgctggaa aacggcgtgc gcatcctggt
      481 gccgcccttc gtcgtcgccg gcgagcgcgt ggtggtcgac accaacgagc tcacctacgt
      541 ccgccgcgcc gagtaggaaa gttcacttcg gtccagcata gcgtcatacc ggcgaaagcc
      601 ggtatccagg aaacgcgccg ccgattcttg atttgctgga ttccgcttgc gccggaatga
      661 cgtaaagagg cggcggaacc gaaagagaga tcaatgcctg cgtctgcctt gatgaatgtg
      721 atagtcggcg ccgcgcgcaa agccggccgc agcctcgcgc gcgacttcgg cgaggtggag
      781 cagcttcagg tctcgatgaa aggcccggcc aacttcgtct cagccgccga cacccgcgcc
      841 gaggagatcc tgcacaagga gctgttgcgc gcccgcccag gctacggtct cctgctcgag
      901 gagcacggcg aggtggaagg cgccgatcgc acccaccgct tcatcgtcga cccgctcgac
      961 ggcaccacca attttctgca cggcatcccg ctgttcgcca tcgccattgc gctcgaacgc
     1021 gacggcgagc tggtggccgg cctcatctac aatccggcca gcaacgagat gttcaccgcc
     1081 gagcgcggca aaggcgcctt cctcaacgac cggcgcatcc gcgtcgcggc gaggaccgag
     1141 cttgccgatt gcgtcatcgt caccggcatt ccgcatcgtg gcaaaccagg ccaggacctt
     1201 ttcctgcgcg agatggaagc cgtcatgtcg gtcacggccg gcgtcaggcg caccggcgcc
     1261 gcggcgctcg accttgcctg ggtcgccgcc ggccgcttcg acggcttctg ggagcggaac
     1321 ttgcgcgcct gggatctcgc cgccggcatc gtcatcctgc gcgaggctgg cggtttcgtc
     1381 agcgacgccg acggcaagga caagatgctg gagaccggaa gcgtggtcgc cggcaacgag
     1441 agcatccagc gcaagctgtt gaagcagctt aaggccgccc aagtcccgcg cgacgccgtg
     1501 gccgcagccg atccgtcacg ctagcgacac ccacggcggc gagattgatc gagattgata
     1561 tcgtcgcgcc ctgaggttgc cgttttgcca ttcttagtat tcagttaatc tctgcccgca
     1621 accgcccccc gattccctga acggcaccat ggcagcgacg tcgacctaca aggccaagct
     1681 cacctacccg caaatcttcc tctggcggat gagtctgttc ctcatcctgg cgatcctgct
     1741 cgccgtcatc ctcgacgggc agacgcagca gttgcgcacg agcttcctgg ccaatcccgg
     1801 cctgaacagc ctcattctcg gcgtcctgtt cgtcggcgtc gtctatgcct ttcgccaggt
     1861 gctgcgccta tatccggaga tccgctgggt caacagcgtc cgcatttccg accccggtct
     1921 cgccgtcgat acgacaccga tgctgctcgc gcccatggcg accatgctcc gcgaccgcac
     1981 cggccatctg tcgcttgcca ccggcgccat gcgctcgttg ctcgactcgg tcgcctcgcg
     2041 cctcgacgag cagcgcgaga ccacgcgtta cctcgtcggc ctgctcgtgt ttctcggcct
     2101 gctcggcacg ttctggggat tgctgcagac cgtgacctcg gtcggctcca ccatcagtgc
     2161 gctcgacacc aacggcagcg aaagcaccat gctgttcgat cagctgaaag agggccttgc
     2221 cgcgccgctc aaaggcatgg gcaccgcctt ctcctgctcg atgttcggcc ttggcggatc
     2281 gctgatcctc ggctttctcg atctccaggc tggccacgcc cagagccgct tctacaacga
     2341 gctcgaagat tggctctccg gcatcaccga gctgcaatcg gcggaaggca cgaccgtcgc
     2401 cggcgtgccg ccccagctcc gcttcgccct cctcgacatg cagcgctcgc tgtccgacct
     2461 gagcgagaag atcgagctcc gcgtgcttgg cgacaacacc ggcgacgcgg tgaaggatct
     2521 cgcccagggc gtggagaagc tgatcaggca gatgcgcgcc gagcagaagg tggtgcgcga
     2581 atgggccgac gagcaggccc agcatcagca tgagctcgcc gtggccctga agcagatcac
     2641 cggcaaggtg aagctgccgg tgcatgagga agagcggagc tagccatggc gctggcaaag
     2701 tcccgccgcg gccccgccgt caactattgg cccggctatg tcgacatgct ctccaccctg
     2761 ctcctggtgg tgaccttcct catgtcgctg ttcatgctct cgaactactt catcaccacg
     2821 cagacgagcg gcaaggacac catgctgtcg cggctgaacc gccagctgtc gcagctcacc
     2881 gaacttctcg cgctcgagcg ctccaagaag gagtcgattg aggacagtct ctccgcgctg
     2941 caagcaacct tgatcgacaa ggacaaggag aatgcgcggc tgactgggct gctcggcgat
     3001 cagagcggca agaccgaggg cgccgaggcc aagaccagcg cgctatcagg ccagctcgac
     3061 cagcagaagc agatcaccaa cgaggcgctg gccaaggtcg agatcctcaa tcagcagctc
     3121 gcggccctca ggcttcagct tgcggctttg caggaggcgc tcgacgcctc cgaggctaag
     3181 gaccgtgaga gccaggtcaa gatcgccgat ctcgggcagc ggctgaacgt ggcgctcgcc
     3241 aagaaggttc aggagctcgc ccgctaccgc tccgatttct tcggcaagct gcgcgaggtt
     3301 ctcgggtccc gtgccgattt ccaggtggtc ggcgaccgct tcgtgttccc ggccgacgtg
     3361 ctgttcgact caggctccgc cgatctcaag cccgaagcca cgccccagat cgacaagctc
     3421 gccgatgcgc tgaaacagct cgagacgcag atcccgcccg acatcgcctg ggtgctcagg
     3481 atcgacggcc acaccgacgt gaagccgatc accaacgtga acttcccgtc gaactgggag
     3541 ctgtcctcgg cgcgcgccat ttccgtggtg cgttatctga ttgaccacgg cattccggcg
     3601 aaccggctgg tcgccgccgg cttcggcgaa ttccagccgc tcgatcccgg cgactccgac
     3661 gaagccttgg ccaagaaccg ccgcatcgag ctcaagctca ccgagcgcta gccctccctc
     3721 cctgcgtcct ttggtctaag cacggcagct tgacgcgagg tggcgatgtt atgttatatc
     3781 atcgcaatga acgccgccca tcaccatgac cacccgaagc tgtcgcgcaa tcaggggcaa
     3841 gtcctctctg ccttgcgcaa ggccaaggag ccgttgagcg cctacgcgat cctcgaccgg
     3901 gtgaggacct ccggcatctc ccatccgccg accgtgtatc gcgccctgaa cgagctgatc
     3961 gggctcggca tggtgcatcg gctgcaatcc cgctctgcct tcgtcgcctg cgaccacggc
     4021 gcctgcaacg gcaaggccgc cttcgccatt tgccgccaat gcgagaaggt cgtcgagatt
     4081 tcgctgagtg acaaagacga gcgggtgctg ctcgcgctct cgccgaagga catcgcggcg
     4141 gagcagatca cgctcgaaat cgccgggctg tgcgaggcct gccgcccggc caaggcgacc
     4201 tgagcccagc cataaccttt gcaacgccat tggtagggag gcgcgtccat ggaccagaga
     4261 agcggcatcg gccatgagca cgcggacgag cacgcgctca tgcctccgcg acaaggcttc
     4321 tcgctcttgc gcctttcggt gccggcacgc ctcgccatgg tcggcgccat caccgccctc
     4381 ctctgggcat tcgtttggct gtgggcgctc cgatgaccca agagaagccg ccggccatca
     4441 gctttcacga tttgacgctc tgctacgacc gccaccccgc cgtgcaccac atcaccggca
     4501 acgtcgagcg cggcgatctg ctcgcgctgg tggggccgaa cggcgccggc aaatcgacct
     4561 tgctcaaggg cattatcggg cagctcaagc ccctcgctgg cagcctcagc ctcgatgggc
     4621 tgaaacggaa cgacatcgcc tatttgccgc aacagatcga catcgaccgc agcttcccga
     4681 tcacggtgtt cgacgccgtt tccatgggtc tttggcgcca gatcggcacg tggcgcggcc
     4741 ttgaccgcgg gctcaacctc gcggtcctcg aggcgctcgc cacgctcggc ctgcaagact
     4801 taagcaaacg gagcgtcggc gcgctctcgg gcgggcagtt ccagcgcgtg ctgttcgccc
     4861 gcctgctgtt gcaggacgcg gccatcatcc tgctcgacga gccgttccgt gccgtcgaca
     4921 ccaagacggt cgaggatctg ctgcgcctca tcagccgctg gcatggcgaa ggccgcaccg
     4981 tgattgccgc gctccacgat ctcgatcagg tgcgcgagca cttccccaaa acgctgctgc
     5041 tcgcccgcga ggtcgtggcc tggggaccga ccaagcaggt gctgacggcg aagaacctcg
     5101 tcaagtcgcg gcagctcacc gaggcatggg acgagcacgc ggaaatttgc gagcgcggcg
     5161 aagctcgaac cgagcggatc tcggcatgat ccatgccctc atcgatccct tcgtcgaatt
     5221 cgccttcatg cgacgggcgc tggtcggctg ctttgccctc tcgctcggcg cgccgccggt
     5281 cggcgtattc ctcgtgctcc gccgcttgag cctgatgggc gacgccatga gccacgccat
     5341 cctgcccggc gcggcgatcg gcttcctcgt cgccggcctc tcggtgccgg ccatgagcct
     5401 tggcggcttt atcgctggca tcgccgtcgt cctgctcgcc ggtctcgtca cccgctccac
     5461 catcctgcgc gaggacgcga gcctcgccgc cttctatctc gtctcgctcg cgctcggcgt
     5521 gctgatcgta tcgatgaaag gcagcagcat cgacctcttg cacgtcctgt tcggcacggt
     5581 gctcgcgctt gacgataccg cgctgatcct gatcgccgcg atcgccaccc tcaccatggc
     5641 gacgctcgcc atcatctacc gaccgctcgt gctcgaatgc ttcgatccga gcttcctcgc
     5701 ctcggtcagc cctttgagct ctcccactca cttcctcttc ctggtgctcg tggtgatgaa
     5761 cctcgtgggt ggcttccagg cactcggcac gctgatggtg gtcggcatca tgctcctgcc
     5821 cgcgatcacc gcgcgcttct gggcgcaaga catatcgaac atgatcatca tcgcggttgg
     5881 cgcggccttc gcggcaagct tcatcgggct gctgctctcc tattacttaa acctgcccac
     5941 cggcccggcg atcatcctca ccagcggcgc gggctatctc atctccatgc tcatcggcac
     6001 ggagggcggc cttctgtggc agctcgtgcc gcgcaaacac ctggaagctt gaatcaaaga
     6061 ctaggagttc gtgtcatgca tagacgctcg tttctcatcg ctgcggcagc ccttgccgca
     6121 atgctcggcg ctaccgctgg agcccaagcc gccgagccga tgaaggtctc ggcctcgttc
     6181 agcatcctcg gcgacatggt ccgccaagtc ggcggcgacc gcgtcgaggt gttcaccttc
     6241 gtcggaccaa acggcgacgc tcacgtttat gagccgacgc cggccgacgc caaggagctg
     6301 tccgaatcga agatcctgtt catcaacggc ctcggccttg agggctggat gacgcggctc
     6361 gagaaatcgt caggcttcaa agggaccgtg gtgacggcaa gcaagggcgt ggcaccgcgc
     6421 gagatggtcg aagacgagaa gaccgtcacc gatccgcacg cctggcaaag cctcgccaac
     6481 ggcaagatct atgtcgccaa tatccgcgat gctctgattg ccgccgatcc agaaggcaag
     6541 gccacctatg aggccaatgc gaaagaattc cttgagcgaa ttgccgccat ggagaccacg
     6601 gtgaaagagg cgatcgccaa gcttccgcct gagcgccgca agatcatcac cacgcatgac
     6661 gccttcggct atttcggcgc ggcctacggc atggagttca tcgcgcctga gggtgtcagc
     6721 accgagagcg aggcttcggc caaggacgtg gccaagatca tccgccagat caaggctgag
     6781 aaggtccctg cggtgttcct ggagaacgtg accgactcta ggctgctcga acagatcgcc
     6841 aaggagaccg gcgccaagat cggcggcacg ctgttttccg acgcgttgtc gcctcctgac
     6901 ggccctgccg ggacctatct cgacatgttc cacaacaata tcgagaccat gtcggaggcg
     6961 ctatcgtcgt agtggcacct catgcccggg cttggtagcg caccctctca gcccaatgcg
     7021 acgatgcgtc cttcttgccc tggcgatcgg ccttgttgcg cttggctggt ctgccgccag
     7081 ccacgccgaa gatcgcaagg agcgggaatt tcaggaatgc ccggaatgcc cggtgatggt
     7141 cggcatcccc gccggcacgt tcgtgatggg aagcccgcac accgagagcg ggcgcttcga
     7201 ttcggagggt ccgcagcacg aggtcgcgat caaagccttt gccctcggaa aatatgacgt
     7261 caccagcgag caatttctca ccttcctcaa ggagaccggc taccagcctc gcccctgcag
     7321 caccatcctc gacatgaagt ggaagtctcc cgggcatggc gtggcctatt cgccgctgaa
     7381 cggagagccg ctgcattggc cggcggtctg cttggattgg cacgatgctg aaaaatatat
     7441 cgcctggctg aacgacaagg tgcgcatcgc ccaccccact ctcgatcacg ccaagggtgc
     7501 ctatcgtctg ccgacggaag ccgagtggga gtatgccgcg cgcgccggca ccaccacggc
     7561 gcgctcgtgg ggcaacgaca tcggcgcggg caaagccaat tgcaacggct gcggcagcaa
     7621 gtgggacaac cggctgttcg ccgacgtcga cagctttgcc cccaaccctt tcggcctcta
     7681 cggcatgctc ggcaacgcct ggcaatggac cgccgattgc tggcacccga attatttggg
     7741 cgcgccgcgc gacgggaacg cgttggtcga ggcggattgc gacaagcacg tcattcgcgg
     7801 cgggtcgtgg agcaatcttc cgaatttcgt gcgctcggct gcgcggagcg gcagcggccg
     7861 caacagcggc gagtacgact attcgagcct caccggcttc cgcgtcgcgc gcgacttgcc
     7921 gtgatcagcg caaagcgtct ttcggattga ggtagagcac catgtccatc acctcctggt
     7981 aatggcctgc cggcagcgca atcggcgacg acaacagcac cgcgttgcgg gcactgacgg
     8041 cgatctcgcg aaagtcggga tctgccgcgc gggcggtatc gacgatctcg gccttcagca
     8101 cgacgccggc gctggtgatc tcgacatgga tcggcaccga cacttcgtgg ttgcgcaagt
     8161 tcgcgagatc aggattccaa cggcgctcga cttgggcgcg gatgtagtcc ttcacgctga
     8221 acggcccctc aagtcctgat gcgatgtcgt cattcttcgc caccctgtca ggtctcgcgg
     8281 cgccgttctc ctcgctcggg gtcaccgctt cgggctggcg cagcttcgcc agagcctcga
     8341 gcttggcttt caactcgtcg gcgaccggct cgctcgctcg cgccgcccca tgcgccgttg
     8401 cgtctgacgg gtgagcagcc tctggctgcg gcaacgctgc cgtcggcggc tcggacgcgg
     8461 ctgatgcgct ctgattcgtg atttcgacct cgaccggcac gatctcgagg ccaccttgcg
     8521 atccttccgt gccgaacacg ccaacaacga ggagcaggca tagagcgatg ccatgcagca
     8581 tcacggatag cgccagcccc gcggcgctct cgcgcccttg cgtctcgctt gggccgatag
     8641 ggcccacagg ctcgacccgc aagggctctt catccccaag gtccatggcc cattctaacc
     8701 cacttccctc ctccgcggcg cttcttgccg agcctgtcat gaagccgcgg aagcctgccc
     8761 tggcgcggcg gcaagaaatc tgatgtatcc ttgagccatg gagagttccg cgcgaaggcg
     8821 ctcagttccg gtcaatattg gcggcgtcat tgtcgggggc actgcgccaa tcgtggtgca
     8881 gtccatgacc aacaccgaca ccgcggatat cgatgccacc gtggcccagg tggcagccct
     8941 tgcccgcgcc ggctcggagc tcgtgcgcat caccgtcgat cgcgacgagg ccgctgccgc
     9001 cgtgccgcat atccgcgagc ggctggacgc gcgcggggtc gacgtacctc tcatcggcga
     9061 cttccactat aacggccaca ctttgctcgc cgaatatccg gcttgcgcgc aagcgctcgc
     9121 caaataccgc atcaatcccg gcaatgtcgg cttcaaggac aagcgcgacc ggcagttctc
     9181 ggccatcgtc gacatcgcca tcaggaacga caagcctgtg cgcatcgggg tgaattgggg
     9241 aagcctcgac caggatctac tcacccgcct catggacgag aatgcgaaac tgcccgagcc
     9301 caaggacgcc cgcgccgtga tgcatgaggc gatcgcgcaa tccgcgctgc tcagcgccga
     9361 gcgcgccgtc gagctcgggc taggccccga caagatcgtg ctctcggcca aggtgagcgc
     9421 tgtaccggac ctgatccagg tctatgccat gctcgccgcg cgctcagacc tcgcgctgca
     9481 tctcgggctg accgaggccg gcatgggctc gaagggcatc gtcgcttcct ccgccgcgct
     9541 cgccgtgctg ctgcagcaag gcatcggcga caccatccgc atctcgctca cgcctgagcc
     9601 gaacggcgac cgcacgctcg aggtgaaggt ggcccaggaa ctgctgcaaa gcatgggctt
     9661 ccgcagcttc gtgccgctgg tcgccgcctg ccccggctgc gggcgcacca cctcgaccac
     9721 cttccaggag cttgcccgcg acatccagga cttcatccgc gacgagatgc ccgagtggaa
     9781 gcgccgctac ccaggcgtcg agacgatgac cgtcgcggtg atgggctgca tcgtcaacgg
     9841 gcctggcgag tcgaagcacg ccgatatcgg catctcgctg cccggcaccg gcgaggcgcc
     9901 cgcggcgcct gtgttcatcg acggcaggaa ggttgccacg ttgcgcgggc ctggcgctgc
     9961 ggcaga
//
LOCUS       DATNAE010000280         3037 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3284572, whole genome shotgun sequence.
ACCESSION   DATNAE010000280 DATNAE010000000
VERSION     DATNAE010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3284572"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            267..1286
                     /locus_tag="VN518_06335"
     CDS             267..1286
                     /locus_tag="VN518_06335"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HXE04226.1"
                     /translation="MAMKLTPGRIAERFRGGPMFPGLIFPMIPGLTFRPSHGGLLPFT
                     PRWRSRPRRLAAWHPPKIYGRIGSLEVRLARKKSEIRQAQRLRYKVFYEEMSAVPGAL
                     ATLSRRDEDAFDPIFDHLLVVDHGDPNDGRRWRRARVVGTYRVLRQEVADRYDGFYTQ
                     GEYNILPLIQAKGAGYSFMELGRSCVLKAYRNRRTLELLWQGVWSYLREHGANVMIGC
                     ASFAGTDPKAHATALSYLYHTALAPEDWRVKAHEHLRVDMNMMPADAVNVRAAIKALP
                     PLIKGYLRVGAYIGDGAVIDHQFGTTDVFVIMPVEAINTRYFAHFGSPDELSAPIPAE
                     FGALN"
     gene            1345..2439
                     /gene="hrcA"
                     /locus_tag="VN518_06340"
     CDS             1345..2439
                     /gene="hrcA"
                     /locus_tag="VN518_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HXE04227.1"
                     /translation="MRHTESTAFRPASDGAVGLRELNERSREIFKRIVETYLTTGEPV
                     GSRNLSRALPMTLSPASVRNVMSDLEALGLIYAPHTSAGRLPTQSGLRLFIDGLLEIG
                     EVSEEERSHIEARIAGNGRHRAVEEFLAEAGAMLSGLSGCAGVVLVDKLSKTLKQVEF
                     VNLGPGRALAVLVSEDGDVENRIIALPEGLPFSSLTEASNYINARIAGLTLAEARSRI
                     EKEIEQRRAQLDELTARVVTEGLATWSGSGDEQKSLIVCGRSHLLEDVRAMEDLERVR
                     LLFEDLDTKKDLIQLLGLAERAEGVRIFIGSENKLFSLSGSALIVSPFEDTEQKIVGV
                     LGVIGPTRLNYARIIPMVDYTARLLGRLVP"
     gene            2502..>3037
                     /gene="grpE"
                     /locus_tag="VN518_06345"
     CDS             2502..>3037
                     /gene="grpE"
                     /locus_tag="VN518_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018045804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HXE04228.1"
                     /translation="MSDEKTDARPEDQAGLANKDAQAPDPAGQASAELTALVAENSDM
                     RDRLLRTMADMENLRRRTEREKADTARYAISNFARDVLTVGDNLRRTIEHVPGDAAAQ
                     DPALKSFLDGVELTERELLNVLERHGVTRIDPVGQRFDPNCHQAMYEVQNADVPEGTV
                     VDVMQAGYVIGDRCLRPAL"
BASE COUNT          535 a         1020 c          945 g          537 t
ORIGIN      
        1 ccacatcgcc gaggcgctca gctatcgcgg cgaaactctg cgccgcgcgc aagctgcagc
       61 gtgagtgcgg ccgagctctg gataaacatt aatcttgggt gaaaagccga gccgcccccc
      121 aatatcccca aactgtcaca cagggttcga attggtttgc taaccgggcc ttgttgtggc
      181 cgaatcgggg ctgccgcgcc agttgcgttg cttcagtcgc gtcgcgtcgc gcggccgcct
      241 caattcacag cgagcctggc tgagacatgg caatgaagct gacacccggg cgcatcgcgg
      301 agcgattccg cggcggcccg atgtttccag gcttgatctt tcccatgatc ccaggactga
      361 cgtttcggcc ttcgcacgga ggtctcctgc cctttacacc gcgctggcgg tcgaggccga
      421 gacgcctcgc ggcctggcat ccgccgaaaa tctacggccg catcggctcg ctcgaagtgc
      481 ggctcgcgcg caagaagagc gagatcaggc aggcgcaacg cctccgctac aaagtgttct
      541 acgaggagat gtcggcggtg cccggcgcgc tcgccacgct gtcgcgccgc gacgaggacg
      601 ccttcgaccc catcttcgat catctgctcg tggtcgatca cggcgacccc aatgatggtc
      661 gccgctggcg ccgtgcgcgc gtggtcggca cctatcgcgt gctgcgccag gaggtggcgg
      721 accgctatga cggcttctac acgcaaggcg aatacaacat cctaccgctg atccaggcga
      781 agggcgctgg ctacagcttc atggagctcg gccgctcctg cgtgctcaag gcttaccgca
      841 ataggcgcac gctcgagctc ttgtggcagg gcgtgtggtc gtatttgcgc gagcacggcg
      901 ccaacgtcat gatcggctgc gccagcttcg ccggcaccga cccgaaagcg catgctacgg
      961 cgctgagtta tctctaccat accgcgctcg ctccggagga ctggcgcgtc aaggcacacg
     1021 agcacctccg cgtcgacatg aacatgatgc ctgccgacgc ggtcaacgtg cgcgccgcga
     1081 tcaaggcact tccgcccttg atcaagggct atctcagggt tggggcctat atcggcgacg
     1141 gcgccgtcat cgaccatcaa ttcggcacga ccgatgtctt cgtgatcatg ccggtcgaag
     1201 cgatcaacac ccgctacttc gcccatttcg gctcgcctga tgagctttcc gctccgattc
     1261 ctgcggagtt cggcgcgctc aactagcttc ctgccaagac cggcatttga cgcaagccca
     1321 gcccgtcctt agatttcggg cgtcatgcgt cacaccgaga gcaccgcctt tcggcccgcg
     1381 agcgacggcg ccgtcggctt gcgcgagctc aacgagcgct cgcgcgagat cttcaagcgc
     1441 atcgtcgaga cctatctcac caccggcgag ccggtgggct cgcgcaatct gagccgcgct
     1501 ctgcccatga cgctgtcgcc ggcctcggtg cgtaacgtca tgtcggacct cgaggcgctc
     1561 ggcctcatct acgctccgca caccagcgcc ggcaggcttc ccactcagtc tggcttgcgc
     1621 ctgttcatcg acgggctgct cgaaatcggc gaggtgagcg aggaggagcg gagccacatc
     1681 gaggcccgca tcgccggcaa tggtcgtcac cgcgcggtcg aggaattcct cgctgaagcc
     1741 ggcgccatgc tgtcagggct ttccggctgc gccggcgtgg tgctggtcga caaactgagc
     1801 aagacgctga agcaggtcga gttcgtcaat ctcgggccgg gcagggcgct cgccgtgctg
     1861 gtgagcgagg acggcgatgt ggaaaaccgc atcatcgcgc tgccggaggg cctgccgttc
     1921 tcgtcgctca ccgaagcatc gaactacatc aacgcgcgca tcgccgggct cacgctcgcg
     1981 gaagcccgct cccgcatcga gaaggagatc gagcagcgcc gcgcccagct cgatgagctg
     2041 accgcccgcg tggtcaccga ggggcttgcc acctggtcag gcagcggcga tgagcagaag
     2101 agcctcatcg tgtgcgggcg ctcccatctc ctcgaagacg tgcgcgccat ggaggatctc
     2161 gagcgggtga ggcttctgtt cgaggatctc gacaccaaga aggacctgat ccagctgctc
     2221 ggccttgccg agcgcgccga gggcgtgcgc atcttcatcg gatcggagaa caagctgttc
     2281 tcgctctcag gctcggcgct gatcgtgtcg cccttcgagg acaccgagca gaagatcgta
     2341 ggcgtgctcg gcgtcatcgg cccgacgaga ctcaactatg cccgaatcat tcccatggtg
     2401 gactacacgg ccaggctgtt ggggcgcctt gtcccttgat ttttctggcc tcacggtcga
     2461 tatgccctga aggttccggt atcccttcgt taagagagcc aatgtccgac gaaaaaaccg
     2521 acgcgcgtcc cgaagatcaa gcgggacttg ccaacaaaga tgcccaggcg cccgatcctg
     2581 cgggccaggc gagtgccgag ctgaccgcgc tcgttgccga gaactccgac atgcgcgatc
     2641 gcttgctgcg caccatggcc gatatggaga acttgcgccg gcgcaccgag cgcgagaagg
     2701 ccgacaccgc ccgctacgcc atctccaatt ttgcccgcga cgtgctcacg gtcggcgaca
     2761 atctccgccg caccatcgag catgtgccgg gcgatgccgc cgcccaggat ccggcgctca
     2821 agagcttcct cgacggggtg gagctgaccg agcgcgaact cctgaacgtt ctcgagcgcc
     2881 acggcgtcac ccgcatcgat cctgtcggcc agcgtttcga tcccaactgc caccaggcga
     2941 tgtatgaagt gcagaacgcc gacgttccgg aaggcaccgt ggtcgacgtg atgcaggccg
     3001 gctacgtgat cggcgatcgc tgcctgcgcc cggcgct
//
LOCUS       DATNAE010000281         2242 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2088085, whole genome shotgun sequence.
ACCESSION   DATNAE010000281 DATNAE010000000
VERSION     DATNAE010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2088085"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /gene="sucA"
                     /locus_tag="VN518_06350"
     CDS             complement(<1..993)
                     /gene="sucA"
                     /locus_tag="VN518_06350"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF027404.4"
                     /note="SucA; E1 component of the oxoglutarate
                     dehydrogenase complex which catalyzes the formation of
                     succinyl-CoA from 2-oxoglutarate; SucA catalyzes the
                     reaction of 2-oxoglutarate with dihydrolipoamide
                     succinyltransferase-lipoate to form dihydrolipoamide
                     succinyltransferase-succinyldihydrolipoate and carbon
                     dioxide; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HXE04229.1"
                     /translation="MSRQDQNPFLYGSNATFIAELYSRFLEDPATVDESWRSFFAELA
                     EQAPDVLKELEGPGWGNARGRVISNGHAEPAAGNGHAAVAAAVESAAPETRQATLDAL
                     RTFQIIRAYRVRGHLLADLDPLGLIRMTEHPDLDPKTYGFTEADYDRPIFVNGVLGFQ
                     SATLRAIIDALKATYCGHVGVEYMHMQELEQRQWIQGRIEVPRNQTEFTREGKRAILQ
                     RLTEAEMFERFLDRRYTGTKRFGLEGAEALIPALEQVIKRGGQLGMQELVMGMPHRGR
                     LNLLTNVMGKPFAALFSEFQGNPANPEDVQGSGDVKYHLGTSTDREMNGISVHLS"
     gene            complement(1231..2196)
                     /gene="mdh"
                     /locus_tag="VN518_06355"
     CDS             complement(1231..2196)
                     /gene="mdh"
                     /locus_tag="VN518_06355"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HXE04230.1"
                     /translation="MARNKIALVGAGQIGGTLALLAGLKELGDVVLFDVVEGVPQGKA
                     LDIAEASPVEDFDAKVAGGNDYSIIAGSDVVIVTAGFPRKPGMSRDDLIGKNAEVVAT
                     VGQNIKKHCPNAFVITITNPLDVMVWVMRESCGLPHQRVIGMAGVLDSARFRYFLAED
                     MGVSVEDVSAFVLGGHGDDMVPLIRYSTVAGIPLPDLIKMGWTTQERVDKIVQRTRGG
                     GGEIVNLLKTGSAFYAPASSAIAMAESYLRDKKRVLPCAAWLTGQYGVKDLYVGVPVV
                     IGAKGVERVVEIALNGDEKAMFDKSVTAVRNLLEATKKLQAGVKG"
BASE COUNT          362 a          734 c          741 g          405 t
ORIGIN      
        1 agacagatgc acggagatgc cgttcatctc ccggtcggtc gaggtgccca gatggtattt
       61 gacgtcgccc gagccctgga catcctcggg attggccggg ttgccctgga attccgagaa
      121 cagcgcggcg aagggcttgc ccatgacatt ggtgaggaga ttgaggcggc cgcgatgcgg
      181 catgcccatc accagctcct gcatgccgag ctgaccgccg cgcttgatca cctgctcgag
      241 cgccgggatc agcgcctcgg cgccctcgag gccgaagcgc ttggtgccgg tatagcggcg
      301 gtcgaggaag cgctcgaaca tttcggcctc ggtcaggcgc tgcaggatgg cgcgcttgcc
      361 ttcgcgggtg aattccgtct ggttgcgcgg cacctcgatg cggccctgga tccattggcg
      421 ctgctcgagc tcttgcatgt gcatgtactc gacgccaaca tggccgcaat aggtcgcctt
      481 gagcgcgtcg atgatcgcgc gcaacgttgc cgactggaag cccagcacgc cattgacgaa
      541 gatcggccga tcgtaatccg cctcggtgaa gccgtaggtc ttgggatcga ggtcgggatg
      601 ctcggtcatg cggataaggc cgagcggatc gagatcggcg agcagatgac cgcgcacgcg
      661 ataggcgcgg atgatctgaa aggtgcgcaa cgcgtcgagc gtcgcctggc gcgtctcggg
      721 cgccgcgctc tcgacagcgg ccgccaccgc ggcgtggccg ttgcccgccg ccggctcggc
      781 atggccgttc gagatgacac ggccgcgcgc gttgccccag cccgggccct cgagctcctt
      841 gagcacgtcc ggcgcctgct cggcgagctc ggcgaaaaag ctccgccagc tctcgtcgac
      901 cgtcgcgggg tcctcgagga agcgcgagta gagctcggcg atgaaggtgg cgttggagcc
      961 gtagaggaaa gggttctggt cctgtcgcga catagccttt ttccgaccgc atctgccggg
     1021 tgcggatggc tgggccacgg cccccatccg ccttgtcccg gcatgctgta agatgggatt
     1081 tagcggcccg gatgcatagc ccggcaatgg ttcaagtctg gtttacgcgg gtctcctgct
     1141 aacgtccgct gcttcgacgc ccctcaccct gccgcgtctt ccccttctcc ccttcttgcg
     1201 ggagagggtc ggggtgagga gtgcacccgc tcagcccttg acgcccgcct gaagcttctt
     1261 ggtcgcctcg aggaggttgc gcaccgcggt taccgacttg tcgaacatcg ccttctcgtc
     1321 gccgttgagg gcgatctcga cgacgcgctc gacgcccttg gcgccgatca ccacgggcac
     1381 gccgacatag aggtccttga cgccgtactg gccggtgagc caggcggcgc agggcagcac
     1441 gcgcttcttg tcgcgcaggt aggattcggc catggcgatc gcagaggaag ccggcgcata
     1501 gaaggccgag ccggtcttga gcagattgac gatctccccg ccgccgccgc gcgtccgctg
     1561 cacgatcttg tcgacacgct cctgcgtggt ccagcccatc ttgatcaaat cgggcagcgg
     1621 gatgcccgcg accgtcgagt agcggatgag cggcaccatg tcgtcgccgt gcccaccgag
     1681 cacgaaggcg ctgacatcct cgaccgagac ccccatgtcc tcggcgagga aatagcggaa
     1741 gcgcgcgctg tcgagcacgc ccgccatgcc gatgacacgc tgatgcggca ggccgcagga
     1801 ttcgcgcatc acccacacca tcacatccag ggggttggtg atggtgatga cgaaagcgtt
     1861 ggggcaatgc ttcttgatgt tctggccgac ggtggcgacc acttcggcat tcttgccgat
     1921 gagatcgtcg cggctcatgc cgggcttccg cgggaagccc gcggtgacga tcacgacatc
     1981 ggatcccgcg atgatcgagt aatcattgcc cccggcgact ttcgcgtcga aatcctcgac
     2041 cggcgaggcc tcggcgatgt cgagcgcctt gccttgcggc acgccctcga cgacatcgaa
     2101 cagcacgacg tcgcccagct ccttcaagcc ggcgagtaaa gcaagcgtgc cgccgatctg
     2161 cccggcgccg accagcgcga tcttgttccg tgccatccgc gacctctcgt tgattgctat
     2221 tcggcgtgag acggcgggcg tc
//
LOCUS       DATNAE010000282         3696 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3884671, whole genome shotgun sequence.
ACCESSION   DATNAE010000282 DATNAE010000000
VERSION     DATNAE010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3696
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3884671"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1350
                     /locus_tag="VN518_06360"
     CDS             <1..1350
                     /locus_tag="VN518_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015670060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobT"
                     /protein_id="HXE04231.1"
                     /translation="DRMPDTVSDQDAFGRLARDLLAALNLADQLDQTGEEGQEGKEGE
                     PEPSAAEADTEDAGGRQQAEFASDAQADQDSLNDETLDEREEGAADQFDRESDMPDAQ
                     RRPEPWRPNLSVLDNPEAFGYKVFTRAHDEIVGAEELCDADELERLRAFLDKQLQALH
                     GAVARLANRLQRRLLAQQNRGWDFDLDEGQIDAARLARVIVDPMHPLSFKQERETQFR
                     DTVVTLLLDNSGSMRGRPIMVAACSADILARTLERCGVKVEILGFTTRAWKGGHAREQ
                     WLNAGKPAFPGRLNDLRHIVYKSADAPWRRSRRNLGLMMREGLLKENIDGEALAWAHQ
                     RLLARPEQRRILMMISDGAPVDDSTLSVNSGSYLERHLRQVIEEIETRSSVELVAIGI
                     GHDVTRYYRRAVTVTDAEELAGVMTDKLAELFDEDVAWRQFYRAAPGAARAKRRKLN"
     gene            1360..2442
                     /locus_tag="VN518_06365"
     CDS             1360..2442
                     /locus_tag="VN518_06365"
                     /inference="COORDINATES: protein motif:HMM:NF024841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HXE04232.1"
                     /translation="MAFSQAKLWRNTAAAILAIAAFLIAAPSFADKPASVLPSVERLA
                     ITAIPIDFDRDDPDRKQFGKLVWRGGINLYAKSPFFGGYSALIVGPSGKSLLAISDAG
                     TWLRANIDYDGRKLKGLSGASIGPIFGRDGKPLAGDTQQDSEGLALIDGDTSKGTAYV
                     AFERNHRVLRYPFTPDRFGPPDGGLTLPAETKRMSANRGIEAIVPIRVGRLKGTTVLF
                     SERLPDKNGNLRGWLIGGPTPGAIALKNVGGFDITDAAALPDGGILVLERRFRYREGV
                     KMRIRRIGAKDLMPGALISGEVLLEANDSLNIDNMEGIAVHRGASGETVLTLISDDNF
                     SALQRTLLMQFALPDGKPVLAEPSSR"
     gene            complement(2439..2738)
                     /gene="rpmB"
                     /locus_tag="VN518_06370"
     CDS             complement(2439..2738)
                     /gene="rpmB"
                     /locus_tag="VN518_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004679586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HXE04233.1"
                     /translation="MARRCELTGKAVLTGNNVSHAKRRTKRRFLPNLCNVTLESEALK
                     KRVRLTISAAALRSVEHRGGLDAYLLKAGDEELSLKARRLKRDIAKVHSVAAPAN"
     gene            2971..>3696
                     /locus_tag="VN518_06375"
     CDS             2971..>3696
                     /locus_tag="VN518_06375"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HXE04234.1"
                     /translation="MPIARHVLSLAAVAVLTCGLGGAPAGDSAFAEITLGAANRITAV
                     YRVDLAGFNLGNFQLTTVFRGTDYEMRGEGRFSVLQGLIFEWHGTTASKGRFGGTGPE
                     PAMYSLNFEGGDKSQQLRMTFDGGAVTQISRIPKKRFNPHVIPITKEQLEGVLDPMTA
                     AFLYTRSGNPNGDPKVCDQTIPVFDGEARFNLILEPKRTAKLQKNSSTGYSGFAAVCR
                     VMFIPISGYRPDNPGIKLMSETNE"
BASE COUNT          659 a         1246 c         1162 g          629 t
ORIGIN      
        1 gatcgcatgc ccgacacggt ctccgaccag gacgccttcg gccgcctggc ccgcgaccta
       61 cttgccgctt tgaaccttgc cgatcagctc gaccagaccg gcgaggaagg ccaggagggg
      121 aaagagggcg agcctgagcc ctccgcggcg gaggctgaca ccgaagacgc cggcggacgc
      181 cagcaggccg agttcgcctc cgacgcccag gccgaccagg actcactgaa cgatgagacc
      241 ctcgacgagc gcgaggaagg cgcggccgac cagttcgatc gcgagtcgga catgcccgac
      301 gcgcagcggc gacccgagcc ctggcgtccc aacctctcgg tgctcgataa cccggaggcc
      361 ttcggctaca aggtgttcac ccgcgcccat gacgagatcg tcggcgccga ggagctctgc
      421 gacgctgacg agctcgagcg cttgcgcgcc tttctcgaca agcagctgca ggcgctgcat
      481 ggcgccgtgg cgcggctcgc caatcgcttg cagcgacgcc tgctcgcgca gcaaaaccgc
      541 ggctgggact tcgacctcga cgaagggcag atcgacgccg cgcggctcgc gcgcgtgatc
      601 gtcgacccca tgcacccgct ttccttcaag caggagcgcg aaactcagtt ccgcgacacc
      661 gtggtgacgc tgctgctcga caattcagga tcgatgcgcg gccgtccgat catggtcgcc
      721 gcctgctcgg ccgatattct cgcccgcacg ctcgagcgtt gcggggtgaa ggtggagatt
      781 ctcggcttca ccacccgcgc ctggaagggc gggcatgccc gcgagcagtg gctcaatgcc
      841 ggcaagccag ccttccccgg caggctcaac gatctccgcc acatcgtcta caaatctgcc
      901 gacgcgcctt ggcggcggtc gcggcgcaat cttgggctga tgatgcgcga aggcctgctc
      961 aaggagaaca tcgacggcga ggcgcttgcc tgggcgcatc agcggctctt ggcgaggccc
     1021 gagcagcggc gcatcctgat gatgatctcc gacggcgcgc cggtcgacga ttccacgctc
     1081 tcggtcaatt caggctctta tctcgagcgc catctccgcc aggtcatcga ggagatcgag
     1141 acccgctcct ccgtcgagct cgtcgccatc ggcatcggcc atgacgtcac ccgctattac
     1201 cggcgtgccg tgacggtcac cgacgccgag gagctcgccg gcgtcatgac cgacaagctc
     1261 gccgagttgt tcgacgagga cgttgcctgg cgccaattct accgcgcggc cccaggcgct
     1321 gcgcgcgcca agcgccgcaa gctgaactag cggggcgccg tggcgttttc ccaggcgaag
     1381 ctctggcgca acaccgccgc ggcgatcctc gccattgcgg cgtttctcat cgcagcacct
     1441 tccttcgccg acaagccggc gagcgtgcta ccaagcgtcg agcgtctggc catcaccgcc
     1501 attcccatcg acttcgaccg cgacgatccg gatcgcaagc aattcggcaa gctcgtctgg
     1561 cgcggcggga tcaatcttta cgccaagtcg cccttcttcg gcggctactc ggcgctcatc
     1621 gttggccctt ccggcaagtc tctgctcgcc atctccgatg ccggcacgtg gctcagggcc
     1681 aacatcgact atgacgggcg gaagctcaaa ggcctgagcg gggcgagcat cggcccaatc
     1741 ttcggccgcg acgggaagcc gcttgccggc gacactcagc aggactcaga aggcttggcg
     1801 ctcatcgacg gcgacacgtc gaagggcacg gcttacgtcg ccttcgagcg caaccatcgc
     1861 gtcctgcgct atcctttcac ccccgaccgc ttcgggccgc ctgacggcgg gcttactctt
     1921 cccgccgaga ccaagcgcat gagcgcaaac cgcggcatcg aggcgatcgt gccgatccgc
     1981 gtaggcaggc tcaagggcac cacggttctg ttctccgagc gcctgcccga caagaacggc
     2041 aatctccgcg gctggctgat cggcgggccg acgccgggcg cgatagccct caagaacgtc
     2101 ggcggcttcg acatcaccga cgccgcggcc ctgccggacg gcggcatcct cgtgctcgag
     2161 cgccgctttc gctatagaga gggggtgaag atgcgcatcc gccgcatcgg cgcgaaggac
     2221 ttgatgcccg gagcgctcat ctcgggcgaa gtcctgctcg aggccaatga cagcttgaac
     2281 atcgacaata tggaagggat cgccgtgcac cgaggggcct ctggcgagac cgtcctcacg
     2341 ctgatctccg acgacaattt cagtgcgctg cagcggacgc ttctgatgca attcgcgctg
     2401 cccgacggca agccggtgct ggccgagccc agttcgcgct agttggcggg ggcagccacg
     2461 ctgtgcacct tggcgatgtc gcgcttgagg cgccgcgctt tcagcgacaa ttcctcgtcg
     2521 ccggccttga gcagataggc gtcgagcccg ccgcgatgct cgaccgagcg aagtgctgcc
     2581 gcactaatag tgagacgcac gcgcttcttc agcgcctcgc tctccagggt gacattgcac
     2641 agattcggta ggaaacggcg cttggttctg cgcttggcat gactgacatt attgccggtg
     2701 agcaccgctt tgccggtcag ctcgcaacga cgggccatcg tacttagtcc ttgtctctag
     2761 acgtgctagg aagctcggtg atgcgggacg gcaagccaag aagtcgagcg ttctcgcgaa
     2821 atccgggcga gatatacggg cctcattgcc tgcggtcaag gcagtgacca ccggatatct
     2881 caacctcaga attgacgaat tctgcgaata atccgagatt tggctggggg cgcctacctt
     2941 tcggcaccgc cagtcttgag ggttggggcc atgccaatag cgcgacatgt gttgagcctt
     3001 gcggctgtcg cagtcctcac ctgcgggctg ggcggcgccc cggcgggcga ttccgccttt
     3061 gccgagatca ccctgggggc ggcgaaccgc atcaccgcgg tttaccgcgt cgatctcgcc
     3121 ggcttcaatc tcgggaactt ccagctcacc acggtgttcc gcggcaccga ctacgagatg
     3181 cgcggcgagg gccggttctc ggtcctgcaa gggctgatct tcgagtggca cggcaccacg
     3241 gcgagcaagg gcagattcgg agggaccggc cccgagccgg cgatgtacag cctcaacttc
     3301 gagggcggag acaagagcca gcagcttcgc atgaccttcg acggcggcgc cgtcacgcaa
     3361 atttcgcgga taccgaagaa gcggttcaac ccgcacgtca tccccatcac caaggagcag
     3421 ctcgaaggcg tgctcgatcc aatgacggcg gcgtttctct atacgcgctc cggcaatccg
     3481 aatggcgacc cgaaggtgtg cgatcagacc attccggtgt tcgacggcga ggcacgtttc
     3541 aacctcatcc tcgagcctaa gcgcacggcg aagctgcaaa agaattcgtc gacgggctat
     3601 tcgggatttg ctgccgtctg ccgcgtgatg ttcattccca tctcaggcta ccggcccgac
     3661 aatcccggca tcaagctgat gtcggagacg aacgag
//
LOCUS       DATNAE010000283         4139 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_882139, whole genome shotgun sequence.
ACCESSION   DATNAE010000283 DATNAE010000000
VERSION     DATNAE010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4139
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_882139"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VN518_06380"
     CDS             <1..460
                     /locus_tag="VN518_06380"
                     /inference="COORDINATES: protein motif:HMM:NF021640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061810 - NAD glycohydrolase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HXE04235.1"
                     /translation="DAPAPVAHHRVPCREEPMDKPRIFLGSSGKQEKLLQALTRGLSD
                     IARVEPWTTSFNPGTSTLERLLELTQEVDFAAFVFAHDDWTTSPPAASSQPSSGQASP
                     RDNVVFEAGLFGGALGMRRTFILHARGAKLPSDLLGLTCVRYDSAMTPSG"
     gene            457..981
                     /locus_tag="VN518_06385"
     CDS             457..981
                     /locus_tag="VN518_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE04236.1"
                     /translation="MRTVNQKIRKAVENEGQLTRIEGSWWQFSLTERTAHEPSALSLL
                     KISRDKNGALELAGRSWREDGRLSARYWSEAVKEREGSSGLFYYWKGERPLDPNAPQL
                     HGVGEIRLESADRASGYFTTSSDNDPHLNARTSGVYWRADPDDASILDGRDDRRRAAL
                     IAERLTHWKSVRNA"
     gene            complement(1026..1694)
                     /gene="ccmB"
                     /locus_tag="VN518_06390"
     CDS             complement(1026..1694)
                     /gene="ccmB"
                     /locus_tag="VN518_06390"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HXE04237.1"
                     /translation="MKEAFALFRRDVMLAWREGGTIGTALGFYLVVVAITPLGLGPDL
                     NLLSRIAPGVLWVALLLASLLSADRIFHNDYEDGALDVLATGPLPLELVAAVKSLAHW
                     VTTGIPLALLAPVLGLLLNLPIESFPLLVLSMLAGTPAVSFVAGIGASLTLGLRRSGL
                     LLALLVLPLYVPVLIFGVACVSAASVGPASPWPPFLILCALGLASLVLAPIASAAALR
                     NAMR"
     gene            complement(1691..2308)
                     /gene="ccmA"
                     /locus_tag="VN518_06395"
     CDS             complement(1691..2308)
                     /gene="ccmA"
                     /locus_tag="VN518_06395"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HXE04238.1"
                     /translation="MPKKTSLAANAISCQRGGRVLFERLSFALEPGESLLVTGPNGAG
                     KTSLLRLIAGLLPLQAGSIEGGDASVPLPELCHYVGHLNGIKSALTLGENVSFWVDFL
                     GGDGSGVQSAYRMFGLAELKDLPAGLLSAGQKRKLALLRLFAAPRPIWLLDEPSVSLD
                     VSSVKVLDKAIAQHLAQGGIAVVASHVTLKVKFAQELALKLEPLT"
     gene            2575..>4139
                     /gene="acnA"
                     /locus_tag="VN518_06400"
     CDS             2575..>4139
                     /gene="acnA"
                     /locus_tag="VN518_06400"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HXE04239.1"
                     /translation="MTSVAETSLDSFKCRRTLKVGKRSYDYFDLKEAEANGLVGIGRL
                     PFSLKVLLENLLRHEDGRTVTADHIRGVALWLQTRKSDREIAFRPARVLMQDFTGVPA
                     VVDLAAMRDAMSALGGDPRKINPLVPVDLVIDHSVMVDAFGNARAFDVNVEKEYERNG
                     ERYAFLRWGSGGFDNFRVVPPGTGICHQVNLEYLAQAVWTKKENGEALAFPDTLVGTD
                     SHTTMVNGLSVLGWGVGGIEAEAAMLGQPISMVIPEVIGFRIAGKLKEGVTATDLVLT
                     VTERLRRAGVVGKFVEFFGEGLDNLPVEDRATIANMAPEYGATCGFFPIDDETLIYLD
                     ATDRKNSRIALVEAYAQAQGLFRDGDTPDPVFTDTIALNLAEVEPSIAGPKRPQDRVP
                     LARAASAFAEALDKEYGKAHEAGKRVPVPGTNFDLGHGDVVIAAITSCTNTSNPYVMV
                     AAGMVARNAVKRGLQVKPWVKTSLAPGSQVVTDYLERAELQHDLDTLGFNLVGYGCTT
                     CIGNSGPLPEDIS"
BASE COUNT          786 a         1318 c         1357 g          678 t
ORIGIN      
        1 tgacgcgccc gcgcccgtcg cgcaccatcg agtcccctgt cgggaggaac cgatggacaa
       61 gcctcgtatc ttccttggct cgtcaggcaa acaggagaag ttgcttcaag cgctgacgcg
      121 cggtctcagc gacatcgcgc gcgtcgagcc ctggacgaca tccttcaatc cagggacatc
      181 gactctggaa cgcctcctcg aactcacgca agaagtcgac ttcgcggcat ttgtgtttgc
      241 gcatgacgac tggacgacga gccccccggc ggcctcttct cagccttcgt cgggccaggc
      301 ctctccgcga gataacgtcg tgttcgaagc cggccttttc ggcggtgcct tagggatgcg
      361 ccggaccttc attctccatg cacgtggggc gaagcttccg agcgacctcc tcggcctcac
      421 ctgcgtacgc tacgacagcg cgatgacgcc gtccggatga gaacggtcaa ccagaaaatc
      481 cgcaaagcgg tcgagaacga gggtcagctc acgcgcatcg agggctcatg gtggcagttc
      541 tccctgacgg agcgcaccgc gcatgagcct tctgccctga gcctcttgaa gatttcgcgc
      601 gataagaacg gcgcgcttga gctcgcgggc cgttcatggc gagaggatgg aaggctgtca
      661 gcaagatact ggagcgaggc ggtgaaggag cgggaaggct cttcaggcct cttctattat
      721 tggaagggtg agcggccgtt ggatccgaat gcgccgcagc tgcacggagt aggggagatt
      781 cgtctggaat ccgccgaccg cgcctccggg tacttcacga caagctcgga taacgatccg
      841 catctgaacg cgcggacatc gggtgtctat tggcgcgccg atccagatga tgcgagcatt
      901 ttggacggcc gcgacgatcg aagacgtgcg gcgctcattg ccgagcggct gacgcattgg
      961 aagtccgtga ggaatgcctg atcgcctctc aggaactgcc attgtggaat gagggtcagg
     1021 tcatttcacc gcatcgcatt gcggagagcg gcggcggagg cgatgggcgc tagcacgagg
     1081 ctggcgaggc cgagcgcgca caggatcagg aatggaggcc agggcgaggc aggacccacg
     1141 ctcgcggcgg agacgcaggc cacgccgaaa atcagcaccg gcacataaag cggaagcacg
     1201 agaagagcga gcagaaggcc agaccggcgc aatcccaaag tgaggctcgc gccgatgccg
     1261 gcaacgaagc tcaccgccgg cgtgccggcg agcatggaga gcacgagcag cggaaaagac
     1321 tcgatcggca gattgaggag aagcccgagg actggcgcga gcagcgcgag cggaatgccg
     1381 gtggtaaccc agtgcgcgag gctcttcacc gcagcgacaa gctcgagcgg taacggcccg
     1441 gtggccagca catcgagcgc gccgtcctca tagtcgttgt ggaagatgcg gtcggcggaa
     1501 agcaggctcg cgagcaggag cgccacccac aaaacgccgg gcgcgatgcg cgagagaaga
     1561 ttgaggtcgg ggccgagccc gagcggagtg atggcgacca ccacgaggta gaagccgagc
     1621 gccgtgccga tggtgccgcc ctcgcgccag gcgagcatca cgtcgcggcg gaaaagcgcg
     1681 aaggcctctt tcacgtgaga ggctccaact tgagcgcgag ctcctgcgcg aacttcacct
     1741 tcagggtcac gtggctcgcc accacggcga tgccgccttg cgcaagatgc tgcgcgatcg
     1801 ccttgtcgag aactttgacc gacgacacat cgagcgagac cgacggctca tcgagcagcc
     1861 agatggggcg gggggcggcg aacaggcgaa gcagcgcaag cttgcgtttc tgtcccgccg
     1921 agagcaggcc tgccggcagg tccttcagct cggcaaggcc gaacatccga taggcgcttt
     1981 gcacccccga gccgtcgccg ccgagaaagt cgacccagaa gctcacgttc tcgccaagcg
     2041 tgagggcgga cttgatgccg ttcaggtgcc ccacatagtg acaaagctca ggcagaggga
     2101 ccgacgcatc cccgccttcg atgctgccgg cctgcaacgg caggagcccc gcgatcagcc
     2161 gcaacaggct cgtcttgccg gcgccgttcg gcccggtgac gagcaggctt tcgcctggct
     2221 ctagggcgaa gctcaagcgc tcgaacagga cccggccgcc gcgctggcag ctgatcgcat
     2281 tggcggcaag gctggtcttt ttcggcattt gcccggaatt tgcgcaagtt ggtccgaagc
     2341 gaggcaggcg cagcggcgaa gcagttgcgt tctcgcgcat tgtctcgccg ccgcaagcgc
     2401 ccttatatag gcgggagcgc gcctccaata gtccgggatg cttgcccccg cccgggtggc
     2461 ttggccggcg cctcgcgcag gcgattggcc aagcctttgc atatcgcttt gcgtgtcgtc
     2521 tgcctgtcgc caagttggcg ctaaacttgg cgctatgccc gaggagatcc ttaagtgaca
     2581 tcggtggccg agacctcgct cgacagcttc aaatgccggc gaacgctgaa ggtcggcaag
     2641 cggagctatg actatttcga cctgaaagag gccgaggcca acgggcttgt cggcatcggc
     2701 cggttgccgt tttcgctgaa ggtgctgctc gagaatctgc tgcgtcacga ggacggccgc
     2761 accgtcaccg ccgaccacat tcgcggcgtg gctctctggc tgcaaacgcg caagagtgac
     2821 cgcgagatcg ccttccgccc ggctcgcgtg ctgatgcagg acttcaccgg cgtccccgcc
     2881 gtggtcgatc ttgccgccat gcgcgatgcc atgagcgcgc tcggcggcga tccgcgcaag
     2941 atcaatccgc tcgtccccgt cgatctcgtc atcgaccatt cggtgatggt cgatgctttc
     3001 ggcaacgccc gcgccttcga cgtcaacgtc gaaaaggaat atgagcggaa cggcgagcgc
     3061 tacgccttcc tgcgctgggg cagcggcggc ttcgacaatt tccgcgtggt gccgccaggc
     3121 accggcatct gccaccaggt gaatctcgaa tatctggcgc aagccgtgtg gaccaagaaa
     3181 gagaacggcg aggcgctcgc cttccccgac actttggtcg gcaccgacag ccacaccacc
     3241 atggtgaacg ggctctccgt gctcggctgg ggcgtcggcg gcatcgaagc cgaggcggcg
     3301 atgctcggcc agccgatctc tatggtgatc cccgaggtga tcggcttccg catcgcgggc
     3361 aagctgaaag agggcgtgac cgccaccgac ctcgtgctca ccgtcaccga gcggctgcgg
     3421 cgcgcgggcg tggtcggcaa gttcgtggag ttcttcggcg aggggctcga caatctcccg
     3481 gtcgaggatc gcgccaccat cgccaatatg gctccggaat atggcgcgac gtgcgggttc
     3541 ttccccatcg acgacgagac cctcatctat ctcgacgcca ccgaccgcaa gaattcgcgc
     3601 atcgcgctgg tcgaggccta cgctcaggcg caaggcctgt tccgcgacgg cgatacgcct
     3661 gaccctgtgt tcaccgacac catcgccctc aaccttgcgg aggtcgagcc gtcgatcgcc
     3721 gggcccaagc ggccgcagga cagggtgccg ctcgcccgcg cggcaagcgc ctttgccgag
     3781 gcgctcgaca aggaatacgg caaggcccac gaggcgggga agcgcgtgcc ggtgccaggc
     3841 accaatttcg atcttggcca tggcgacgtg gtgatcgccg ccatcacttc ctgcactaac
     3901 acctcaaacc cctacgtcat ggtggctgcc ggcatggtcg cccgcaacgc ggtaaagcgc
     3961 gggctgcagg tcaagccctg ggtgaagacg tcgctcgcac ctggatcgca ggtggtgacc
     4021 gactatctgg aacgggccga gctgcagcac gatctcgaca cgctcggctt caatctcgtg
     4081 ggctatggct gtaccacctg catcggcaat tcagggcctc tgcctgagga catctccaa
//
LOCUS       DATNAE010000284         2004 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_4492553, whole genome shotgun sequence.
ACCESSION   DATNAE010000284 DATNAE010000000
VERSION     DATNAE010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4492553"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..578
                     /locus_tag="VN518_06405"
     CDS             78..578
                     /locus_tag="VN518_06405"
                     /inference="COORDINATES: protein motif:HMM:NF019370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GcrA family cell cycle regulator"
                     /protein_id="HXE04240.1"
                     /translation="MAWNDERVELLKKLWAEGLSASQIASRLGGVTRNAVIGKVHRLG
                     LSGRATSSRSASPRPRRAQVPRQHRAPSLMFGTRGNVALKPVYEEEIEPAPAPIEELY
                     IPPAERATILTLKEAMCRWPIGDPGEEEFHFCGRKTAGSVPYCEHHARLAYQAVPSRR
                     RDRRVA"
     gene            complement(675..1400)
                     /gene="phoB"
                     /locus_tag="VN518_06410"
     CDS             complement(675..1400)
                     /gene="phoB"
                     /locus_tag="VN518_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HXE04241.1"
                     /translation="MLSVTAVNPRAKVLVVEDEEPLALLLRYNLEAEGYAVDVVHRGD
                     EAEVAIAESSPDLIVLDWMLPGISGLELCRRLRTGKDSRTIPIILLTARSEESDRIRG
                     LTTGADDYVVKPFSLPELMARVRAILRRTSPERIAAVLTAGDIELDRESHRVTRNGRQ
                     VHLGPTEFRLLEFLMQSPGRVFSRAQLLDGVWGRNVYVDERTVDVHIGRLRKAMMRGT
                     ESDPVRTVRGAGYAFSDKLQETV"
     gene            complement(1402..>2004)
                     /gene="phoU"
                     /locus_tag="VN518_06415"
     CDS             complement(1402..>2004)
                     /gene="phoU"
                     /locus_tag="VN518_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HXE04242.1"
                     /translation="GRDPDLAAATIQQDEAIDLLEAEIEEQVITMIARREPMAYDLRQ
                     IMAALRISTDLERIGDLGKNIAKRALAVVGEQQPKQLMLGLKHMGELALGQLKEVLDA
                     YIERDADRALRVWYTDEDIDAMYNSLFRELLTYMMEDPRNIGLCTHLLFGAKNIERIG
                     DHATNIAETVYFLVHGRPITDQRPKSDTTSSTPVALRSAG"
BASE COUNT          347 a          618 c          667 g          372 t
ORIGIN      
        1 cagtaaatct atgtgtctga cgccgtctct cggacgagac ggggggattc tgagtcgcta
       61 acgaggaggc aaagaagatg gcttggaatg atgaaagagt cgaactgctg aaaaagctct
      121 gggccgaagg actgagtgcc agccagattg caagtcgctt aggcggagtg acgcgcaatg
      181 cggtgatcgg caaggtccat cggcttgggc tttccggccg tgcgacaagc tcgcgatcgg
      241 cgagcccacg gccgcgccgg gcgcaagtgc cgcggcaaca ccgcgctcca tccttgatgt
      301 tcggcacgcg cggcaatgtc gcgctgaagc cggtttatga ggaggagatc gagcctgctc
      361 cggcgccgat cgaagagctg tacatcccgc ccgccgagag ggcgaccatc ctcaccttga
      421 aagaagcgat gtgccgctgg cccatcggcg atccgggcga ggaggaattt catttctgcg
      481 gccgcaagac ggcaggctcg gttccttatt gcgagcatca cgcccggctt gcctatcaag
      541 ctgtgccctc gcggcgccgc gataggcggg tcgcatagag ctagccactt accggctctg
      601 ccgcttcagc ggatgagccg catggtcaag tggggggaag gatggcgccc gttcggcgcc
      661 tccttttttt gggctcagac ggtctcttgc agcttgtcgg agaaggcgta gccggcgccg
      721 cgcacggtgc gaaccggatc ggattcggtg ccgcgcatca tcgccttgcg cagacggcca
      781 atatggacgt cgaccgtacg ctcatcgaca tagacgttgc ggccccatac gccgtccagc
      841 aattgcgcgc ggctgaacac ccggcccggg ctctgcatga gaaactcgag cagccggaac
      901 tcggtcgggc cgagatgcac ctggcgtccg ttccgcgtca cgcgatggga ctcgcgatca
      961 agctcgatgt cgcctgcggt cagcaccgcg gcgatgcgct ccgggctcgt gcgcctgagg
     1021 atcgcgcgta cccgcgccat cagctcgggc agcgagaagg gcttgactac ataatcgtcg
     1081 gcgccggtgg tgagcccgcg aatgcggtcg ctctcctcgc tccgcgcggt caagaggatg
     1141 atggggatgg tgcggctgtc cttgccggtg cgcagccgcc ggcaaagctc gagcccggag
     1201 atgcctggca gcatccaatc gagcacgatg aggtccggcg agctttcggc gatggccacc
     1261 tcggcctcgt cgccgcgatg cacgacatcg acggcgtagc cctcggcctc gagattgtag
     1321 cggagcagca gcgccaaggg ctcctcgtcc tcgaccacga gaactttggc tctcgggttc
     1381 acggccgtga cgctcagcat gtcagcctgc cgatctcagg gccaccggcg tggagctggt
     1441 cgtgtcgctc ttcggccgct ggtcggtaat gggcctgcca tgcactagga aatacaccgt
     1501 ctcggcgatg ttggtggcgt ggtcgccgat gcgctcgatg ttctttgctc cgaacaggag
     1561 atgggtgcaa agcccgatat tgcgtgggtc ctccatcatg taggtgagga gctcgcggaa
     1621 cagggagttg tacatcgcgt cgatgtcctc gtcggtgtac cagaccctca gcgcgcggtc
     1681 ggcgtcgcgc tcgatatagg cgtcgagcac ctctttcaac tggccgagcg caagctcgcc
     1741 catatgcttt aagccaagca tcagctgctt cggctgctgc tcgccgacca cggcgagcgc
     1801 ccgcttggcg atgttcttgc cgagatcgcc aatgcgctcg agatcggtgg agatgcgcag
     1861 cgccgccatg atctgacgaa ggtcgtaggc catcggctcg cggcgggcga tcatggtaat
     1921 gacctgctct tcgatctccg cctccagcaa atcaatcgcc tcgtcctgct ggatggtggc
     1981 ggcggcgagg tccggatcgc ggcc
//
LOCUS       DATNAE010000285         3343 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2386392, whole genome shotgun sequence.
ACCESSION   DATNAE010000285 DATNAE010000000
VERSION     DATNAE010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2386392"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VN518_06420"
     CDS             complement(<1..180)
                     /locus_tag="VN518_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE04243.1"
                     /translation="MTIGWQIAHSDLVGSRSLANRWRGLGSAIGLALAALFVLPTMAK
                     ADCDVPDMSNGIPEDV"
     gene            complement(225..1559)
                     /locus_tag="VN518_06425"
     CDS             complement(225..1559)
                     /locus_tag="VN518_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006725025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HXE04244.1"
                     /translation="MFGRFSLARPLAAATLCVGLAAGLETSPANADPTAKDVLKVYAD
                     IAQAGYTDSLDTANTLKLAVDAFLAKPTEDNLRAARAAWIAARIPYMQTEAFRFGNAI
                     VDDWEGRVNSWPLDEGLIDYVASSYGTESAENQLYVANVIAHTSLTIGGKTLDTSKIT
                     KELLADKLQEAGGVEANVATGYHAVEFLLWGQDLNGTGPGNGARPASDYDTERCTNGN
                     CDRRAQYLSAATGLLVDDLAWMAAQWAPGGAARKTIEQDSDEAGLTAIMTGLGSLSYG
                     ELAGERMKLGLMIHDPEEEHDCFSDNTHASHFFDALGIRNVYLGTYRRADRSLVTGPS
                     VSDLVKAKAPDVDAEVRAKLDATMEAMNALYLRALTTESYDQMIGEGNEAGNAVVQRV
                     IDALLDQTKSLERAVAALDLKSIEFEGSDSLDNPAKVQGDSAAAGDGATKPN"
     gene            1789..2709
                     /locus_tag="VN518_06430"
     CDS             1789..2709
                     /locus_tag="VN518_06430"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HXE04245.1"
                     /translation="MHLREVDANLLVTLDALLVDASVTRAAERLGRSSSAISHALAKL
                     REIFADELFVRAGQRLVPTAKAMELAPTVHVILAGMESLLRPNKPFDPSETSRDFAVA
                     ASEAGELMLLQPLRQRLRPRAPSVKVGWMPSHREENIDALRNGRCHFVIDIEGAFMQA
                     PDIRVLELFDDTLVTLARQGHPLSKRKAKPDMFAAADHIEVNSLPAIELLDRELGADK
                     LSCNVSSRVSSVLVGLLLALNSDALLTVPASLASILERQFGLARIQPPLGSIAFPLRL
                     MWHSSYERDECHEWVRGEFAHLAREMGSVG"
     gene            complement(2676..2948)
                     /locus_tag="VN518_06435"
     CDS             complement(2676..2948)
                     /locus_tag="VN518_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE04246.1"
                     /translation="MFGRGVLAVSARKGDAVLIDQRETDRWNVEGLFCLFQRERLLDA
                     VDRDGELALELGGVGGTRWRAQQQSGAKHANSRRYSQPTLPISRAR"
     gene            2980..>3343
                     /locus_tag="VN518_06440"
     CDS             2980..>3343
                     /locus_tag="VN518_06440"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HXE04247.1"
                     /translation="MAAVGAMRLVEQKKLALDADVNAKLTSWKVPTNGFDKTHPVTLR
                     GLLSMTAGVGVPGFLGYAVDAPLPNLTQILYGVPPANSPPVTVIAEPGSAYHYSGGGY
                     EIAEALMVGAAKVPFPELM"
BASE COUNT          561 a         1094 c         1083 g          605 t
ORIGIN      
        1 cacatcctcg ggaatgccgt tcgacatgtc gggcacgtcg caatcggcct tggccatggt
       61 gggtaggacg aacagcgccg ccaaagcgag gccgatcgcc gagccaaggc cccgccaccg
      121 atttgcgaga gatcgtgatc ctacaaggtc gctgtgcgcg atctgccaac cgatagtcat
      181 ccccgccccc cggccatttt cacgatgata cacgctcgcg tccactaatt cggtttggtg
      241 gctccatcgc cggcagcggc actatcgcct tgcaccttgg ccggattatc gaggctgtcg
      301 gagccttcga actcgatcga cttgaggtcg agagcggcaa cggcacgctc gagcgacttg
      361 gtttgatcga gcagagcgtc gatcacgcgc tggaccaccg cgttccccgc ctcgttaccc
      421 tcgccgatca tctggtcata ggactcggtg gttagagcgc ggagatacag cgcgttcatc
      481 gcttccatgg tggcgtcgag cttggctctc acctcggcat cgacgtcggg cgccttggcc
      541 ttgaccaagt ccgacacgct cggccccgtc accaatgacc ggtcggcgcg gcgataggtg
      601 ccgagataga cattgcggat gccgagcgcg tcgaagaaat gcgaggcatg cgtgttgtcc
      661 gagaagcaat cgtgctcctc ctcgggatcg tggatcatca gcccgagctt catgcgctcg
      721 ccggcgagct cgccatagga caggctgccg agaccggtca tgatcgcggt gaggcccgcc
      781 tcgtcgctgt cttgctcgat cgtcttgcgc gcggcgccgc ccggggccca ttgcgccgcc
      841 atccaggcca ggtcgtcgac gaggagcccg gtcgcggcgc tgagatattg ggcgcgccga
      901 tcgcaattgc cgttggtgca tcgctcggtg tcgtagtcgc tggccggacg cgcgccgttg
      961 cccgggccgg tgccgttcag atcctgcccc cacagcagaa actcgacggc gtgatagccg
     1021 gtggcgacat tggcctcgac gccgccggcc tcttgcagct tgtcggccaa gagctccttg
     1081 gtgatcttcg atgtgtcgag cgtcttgccg ccgatggtga gcgaggtgtg cgcaatcaca
     1141 ttggcgacgt agagctggtt ctcggcggac tcggtgccat aagaagacgc gacataatcg
     1201 atcaggcctt cgtcgagcgg ccaggaattc actcttccct cccagtcgtc gacgatggcg
     1261 ttgccgaagc ggaaagcctc ggtctgcatg tagggaatgc gggcggcgat ccaggcggcg
     1321 cgcgcggcgc gaagattgtc ctcggtcggc ttggcgagga aggcgtcgac cgccagcttg
     1381 agcgtgttgg ccgtgtccag ggagtccgtg tagccggctt gcgcgatatc cgcgtagacc
     1441 ttcagcacgt ccttggcggt cgggtcggca ttggcgggcg aagtctccag gccggcagca
     1501 aggccaacgc aaagcgtcgc ggcggcgagc ggccgggcca gcgaaaatcg tccgaacatt
     1561 gtcatccctt ttctgccgag gctcctcccc ggtcttggta atgccgcgcc caggccggga
     1621 aatacggcgg ccctccggcc tagaccttcg ggccgccgca aggttgagat gaggaaacat
     1681 ccgcgctttc tcaggcaacc gggctgggca gccgggtgcc ttgtcaaaac cctaccgcaa
     1741 gcgctacatg caggaagtga actcttttca gcagaatagt tgcaagccat gcacctacgg
     1801 gaagtcgatg ccaatctgct ggtgacgctc gacgctttgc tcgtcgatgc cagcgtcacc
     1861 cgcgccgccg agcgtctcgg gcggagttcc tcggccatca gccacgcgct ggccaagctt
     1921 cgcgaaatct tcgccgacga gctgttcgtg cgcgccgggc aaaggctggt gccgaccgcc
     1981 aaggcgatgg agctcgcgcc caccgtccac gtcatcctgg ccggcatgga gagcctgctc
     2041 cggccgaata agccgttcga cccatcggag acctcgcgcg acttcgccgt cgccgcgagc
     2101 gaggccggcg agctcatgct gctgcaaccg ttgcgccagc ggctgcgccc gcgggcgccg
     2161 agcgtgaagg tcggctggat gccttcgcat cgggaggaaa acatcgatgc gctgcgcaac
     2221 ggacgctgcc atttcgtgat cgacatcgag ggagccttca tgcaggcgcc cgacatccgc
     2281 gtgctcgagc tgttcgacga cacgctcgtc acgctggcgc ggcaaggaca cccgctttcg
     2341 aagcgcaagg cgaagccgga catgttcgcc gcggcggatc acatcgaagt gaattcgctg
     2401 cccgcgatcg aactcctcga ccgcgaactc ggcgccgaca agcttagctg caatgtctcc
     2461 tcgcgcgtct catccgtgct ggtcggtctc ctgctcgcgc tgaacagcga tgcgctgctg
     2521 accgtgccgg cctcgctggc gtcgatcctc gagaggcaat tcgggctcgc ccgcatacag
     2581 ccgccccttg gttccatcgc ctttccgttg aggctgatgt ggcattcgag ctatgagcgc
     2641 gacgaatgcc atgaatgggt gcgcggcgag ttcgctcatc tggcgcgcga aatgggaagc
     2701 gtgggttgag agtatcggcg ggaattggcg tgcttggcgc cgctctgttg ctgagcgcgc
     2761 cagcgcgtgc cgccgacccc gccgagctcg agagcaagct cgccgtcacg gtcgacggcg
     2821 tcgagaagac gctctcgctg gaagaggcaa aacaggccct ccacattcca tcggtcagtc
     2881 tcgcgctgat cgatcaggac agcatcgcct ttgcgcgcgc ttacggcgag aacgccacgc
     2941 ccaaacacgc tgtatcaggc cgcctcgctg tcgaaattcg tggccgccgt cggcgccatg
     3001 cggctcgtcg agcagaagaa gctcgcgctc gatgcggacg tcaacgcgaa gctcacctcg
     3061 tggaaggtgc cgaccaatgg cttcgacaag acgcaccccg tgacgttgcg cggcctgctc
     3121 agcatgacgg ccggggtcgg cgtgccgggc tttctcggct atgccgtgga tgcgccgctg
     3181 ccgaacctga cgcagatcct ctacggcgtg ccgcccgcca actcgccgcc ggtcacggtg
     3241 atcgccgaac ccggcagcgc ctatcattac tccggcggag gctacgagat cgccgaggcg
     3301 ctgatggttg gcgccgccaa ggtgcccttc cctgagctga tgg
//
LOCUS       DATNAE010000286         3353 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_581512, whole genome shotgun sequence.
ACCESSION   DATNAE010000286 DATNAE010000000
VERSION     DATNAE010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581512"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="VN518_06445"
     CDS             <1..542
                     /locus_tag="VN518_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004687748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HXE04248.1"
                     /translation="KEAIEIAIIENVQREDLNAVEEAEGYRLLMDGHGYTQEDLSKVI
                     GKSRSHLANTLRLLKLPESVQALLRSGALSAGHARCLIGRADAAALAERIVNEGLTVR
                     QVEALVQDRPAEPAKMRKARSKNADTRAAESELKEALGLDVDIRSGRGEKGELRIRYT
                     SLDQLEDLRHRLLRRPAYR"
     gene            593..847
                     /locus_tag="VN518_06450"
     CDS             593..847
                     /locus_tag="VN518_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE04249.1"
                     /translation="MIQIILKIICSIRLKGEKPMGAGIVLIAVAVLAILCGAAALPRG
                     LLMVAFVTLIATAAVVIVFEVAPTGNSPAVDLNHARSALP"
     gene            complement(1020..1565)
                     /gene="pyrE"
                     /locus_tag="VN518_06455"
     CDS             complement(1020..1565)
                     /gene="pyrE"
                     /locus_tag="VN518_06455"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HXE04250.1"
                     /translation="MDQRRKRLADLIKERSFRRGNFTLASGKQSAYYFDSKPTMLHPE
                     GASLLAALIVDELAGVKADCVGGLEMGAVPLIAPVAMRSLEAGRPLLGFFVRKAPKDH
                     GTRQRIEGLDLAGKTAVILEDVTTTGGSAMQAVEEVRAAGGTVALVLSILDRGEGAAE
                     FYAAAGVPFKSLFRAEEFLRD"
     gene            complement(1641..2684)
                     /gene="holA"
                     /locus_tag="VN518_06460"
     CDS             complement(1641..2684)
                     /gene="holA"
                     /locus_tag="VN518_06460"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HXE04251.1"
                     /translation="MVAYKTGSFQRILKSLEPECRAALVYGPDAGLVAERAAALADAF
                     AGKRKEATEFVRLDEKDLAEDPARLEVELRTIPMFAERKVVRVAAGARLDVPMLKALL
                     EAPLAGALVIEAGALRPDSALRKLFESHKTAAALPSYSDERSASELIDEELDKAGLKI
                     DSDTRSYLMTRLGADQALSRAEVAKLALFASSHGRVAPEDIDAIVGDSAEIAVETFVY
                     EVSGGETKEALRQLGRLAAAGTEPSVALSALGRHFTQLHRVASAQAAGVAIEQALRSL
                     RPRPHFKREKSFVADSRRLGAQRLLEALPLIQDAVKRARLNPDLERAVAERLVLALTR
                     RGDARRASNIGEP"
     gene            complement(2718..3191)
                     /gene="lptE"
                     /locus_tag="VN518_06465"
     CDS             complement(2718..3191)
                     /gene="lptE"
                     /locus_tag="VN518_06465"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HXE04252.1"
                     /translation="MTAAISLAGCGIQPLYGTTAGGSRLAAAMAGVDITPIPGRVGQR
                     VRNELIFENTGGSGQTGTTYKLDIVIKESLTNELVKISGDAKSQVYELDATFKLISND
                     GRVVLEGKATSRAPYERFETIFSNVRARYDAENRAARTVAESIKVRIAAYLSQSA"
BASE COUNT          573 a         1080 c         1079 g          621 t
ORIGIN      
        1 acaaggaagc gatcgagatt gccatcatag agaacgttca gcgcgaagac ctgaacgcgg
       61 tcgaggaggc tgaaggctat cggttgttga tggacggtca cggctatacg caggaagatt
      121 tgtccaaggt gatcggcaag agccgcagcc atcttgccaa cacgctccgc ctgctcaagc
      181 tgcctgaaag cgtgcaagct ttgttgcggt cgggcgcgct cagcgccggc cacgcgcgct
      241 gcctgatcgg gcgcgccgac gccgcggccc ttgccgagcg gatcgttaac gaagggttaa
      301 cggtgcgcca agtcgaggct ctggttcagg atcggccggc cgagccggca aaaatgcgca
      361 aggcgaggtc gaagaacgcc gatacgcgcg ccgcagaatc cgagctgaaa gaagccttgg
      421 gcctcgatgt cgacatccgc tcaggccgtg gcgagaaggg tgagcttcgc atccgctaca
      481 cctcgctcga ccagctcgag gatttgcgcc acaggctcct ccgtcggccg gcctaccgct
      541 aaacgagttc gtcatccgac gaggaagccc tgctctcggc accagctcgc tggtgattca
      601 gattatctta aaaattatct gctcgattcg gctcaagggg gaaaagccga tgggagcagg
      661 tatcgttctt attgcggtcg cggtgttggc aattctttgc ggcgctgccg cgctgcctcg
      721 tggcttgctg atggtcgcct tcgtcacgct gattgccaca gcggcggtgg tcattgtgtt
      781 tgaggtcgct ccgaccggga actctccggc cgtcgatttg aatcacgcgc gatcggcttt
      841 gccctgacgc ccgctatcgc aggtggcgcc gtgcggccac gtagatcaat gccgcggcga
      901 gtgcgccgcc gccgatgagg tcgaggacca acagccccca ggatgatgcg cttagcattt
      961 tccccctcca cccgatttat cttttatgcg cggggcggct taaggaagat tgaagcttgc
     1021 taatcgcgga ggaattcctc ggcgcggaac agcgatttga acggaacgcc ggcagctgcg
     1081 tagaactcgg cggcgccctc cccgcggtcg aggatcgaca agaccagcgc gacggtcccg
     1141 cctgcggccc tcacctcctc gacggcctgc atcgccgagc cgccggtggt ggtgacgtcc
     1201 tccaggatga ccgcggtctt tcccgccagg tcgaggccct cgatgcgctg cctggtgcca
     1261 tgatcctttg gcgccttgcg cacgaaaaag ccgagcagcg gacgcccggc ctcgaggctt
     1321 cgcatcgcca ccggcgcgat gagcggcacc gcacccatct ccaaacctcc gacgcaatcg
     1381 gccttcacgc cggcaagctc gtcgacgatc agcgcggcga gaaggctcgc gccttccgga
     1441 tgcagcatgg tcggcttgct gtcgaaatag taggcgctct gcttgcccga ggccaaggtg
     1501 aagttgccgc gccggaacga gcgctccttg atgagatcgg caagacgttt gcggcgctgg
     1561 tccatgatcg tccccggagg tgacatcgtg cccgcgcggg tacaccggtt tcaatcccgc
     1621 cgcaactggg cttcgcgtga ctatggctcg ccgatgttgg acgcccggcg cgcgtccccc
     1681 cgccgcgtga gcgcgagcac cagccgctcg gcgacggccc gctcgagatc tggattgagg
     1741 cgcgcgcgtt tcaccgcgtc ctggatcagc ggcagcgctt cgagcagccg ctgggcgccg
     1801 aggcggcggc tgtcggcgac gaaggatttt tcgcgcttga aatgcgggcg cggcctgagc
     1861 gaccgaagcg cctgctcaat tgcgacgccg gcggcttgcg ccgaggcgac gcggtggagc
     1921 tgcgtgaaat gtcgcccaag tgccgaaagc gccaccgacg gttcggttcc cgctgcggca
     1981 agtctaccga gttgacgaag cgcctccttg gtttcaccgc cgctgacctc gtacacgaag
     2041 gtctcgaccg cgatctcggc gctgtcgccg acgatcgcat cgatgtcctc aggtgccacg
     2101 cgtccgtggc tgctcgcgaa cagagcaagc ttggcgacct cggcgcgcga cagcgcctga
     2161 tccgcgccga gccgcgtcat gagatagctg cgtgtgtcgc tgtcgatctt gaggcctgct
     2221 ttgtcgagct cctcatcgat cagctcggag gccgagcgct cgtcgctgta gctcggcagc
     2281 gccgccgccg tcttgtggct ttcgaacagc ttgcgcaatg ccgagtccgg cctgagcgcg
     2341 cccgcctcga tgacgagcgc accggcaaga ggcgcttcga gcagcgcctt cagcatggga
     2401 acgtcgagcc gggcaccggc ggcgacacgg acgactttcc tctcggcgaa catcgggatg
     2461 gtgcgaagct cgacctcgag ccgcgctgga tcctccgcca aatctttttc atcgagccga
     2521 acgaattccg tcgcctcttt gcgcttgccg gcgaaagcat cggcgagcgc cgcggcgcgc
     2581 tcggcaacga gtcctgcgtc gggaccgtac acgagagccg cgcggcactc aggttcgaga
     2641 gattttagga tgcgctgaaa cgatccggtc ttgtaggcga ccatggcgaa ggaacggccg
     2701 tcttgccgcg acgcgcttta agccgactgc gagagataag cggcgatgcg gaccttgatg
     2761 ctttcggcga cggtgcgtgc cgcgcggttc tccgcgtcgt agcgggcgcg cacattggag
     2821 aagatggtct cgaagcgctc atagggcgcc cgcgacgtcg ccttgccttc gagcacgacc
     2881 ctgccgtcat tgctgatcag cttgaacgtg gcgtcgagct cgtagacttg gctcttggcg
     2941 tcgcccgaaa tcttgacgag ctcgttggtc aggctttcct tgatgacgat gtcgagcttg
     3001 taggtcgtcc ccgtctgtcc gctaccgccg gtgttctcga agatcagctc gttgcgcaca
     3061 cgctggccga cacggccggg gatgggggtg atgtcgacgc cagccatggc cgcggccaag
     3121 cgcgagcctc cggcggtcgt gccgtaaagc ggctggatgc cgcagccggc caagctaatg
     3181 gccgcggtca cagccaaagc ggcaagcaat ggcctatgcc accacattga cgatcctctg
     3241 cggtacgatc acgaccttct taatttgacg tcccccaatg gcgcgcacga cattgtcgag
     3301 ctttagcgcc gcagcttcaa tatcttcctt cgccgcgtcc cgcgcaatcc tca
//
LOCUS       DATNAE010000287         3168 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2089703, whole genome shotgun sequence.
ACCESSION   DATNAE010000287 DATNAE010000000
VERSION     DATNAE010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2089703"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1059)
                     /locus_tag="VN518_06470"
     CDS             complement(<1..1059)
                     /locus_tag="VN518_06470"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2'-deoxycytidine 5'-triphosphate deaminase"
                     /protein_id="HXE04253.1"
                     /translation="MTETNMQPATPRDRLFEALPEEALADPVDGPRSVFARTGILPSQ
                     AIRAMIAGGEIAGAPEIETNQIQPASLDLRLGKVAYRIRASFLPGPNATVRDKLSALK
                     FHEIDLTKGAVLEAGCVYLVPLIEQLKLRSTIAGFANPKSSTGRLDVFTRLIADRQDG
                     FDSVEEGYSGPLFTEICPRTFSIKVRYGSRLNQIRFRRRVGQQLDAGEQPGRVGDRAL
                     REIHSDVGLVEGEAAIRDGLNLSISLAPVLPSGLVGYRARRYAGVIDVDEIGAYDVGQ
                     YWEEVRLGNDHRLVLDPHEFYILASKESVSVPPAYVAEMAPFDPLIGEYRVHYAGFFD
                     PGFGYTEGKAPGAKAVLEV"
     gene            1170..1604
                     /locus_tag="VN518_06475"
     CDS             1170..1604
                     /locus_tag="VN518_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008835644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1772 domain-containing protein"
                     /protein_id="HXE04254.1"
                     /translation="MLIGHLALVVAALFTGAAIYVNFAEQPARLQLDDRSLLAEWKPS
                     YQRGFAMQASLAMLGFLLGAVAWWQTGLAAFLLGALYILAPWPWTLLVIKPVNDALMA
                     TDLAAAGPETRALIVKWNRLHAVRTLLGAAAVISFLFAVSTG"
     regulatory      1646..1723
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00521"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00521"
     gene            1814..3022
                     /locus_tag="VN518_06480"
     CDS             1814..3022
                     /locus_tag="VN518_06480"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015319287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HXE04255.1"
                     /translation="MAANKTRTKGASAWQPATRLVHEGTLRSQFGETSEAIFLNSGYI
                     YASAEQAEARFKGDEDGYVYSRYANPTVSMFETRMCALEGADAARGTSSGMAAVAASL
                     LCHLKTGDHVVSARALFGSCRYIIEDLLPRYGIETTLVDGRDLKAWEKAVRPNTRAFF
                     FETPTNPVLELVDIEAVANIAKQAKALVVVDNVFATPLLQRPLQLGADIVVYSATKHI
                     DGQGRCLGGVVLGPQEFIEEKLHNYLKHTGPSLSPFNAWVLLKGLETLPVRVERHIAS
                     AAAIADFLAARKDVARVYYPGRADHPQAALAKRQMAAGGPLVAFEVKGGKDAAFRCMN
                     ALEIIKISNNLGDAKSLVTHPATTTHQRLAPEIRAELGIFDNSVRLSVGLEDAGDLMA
                     DLDQALAAAK"
BASE COUNT          539 a         1044 c         1009 g          576 t
ORIGIN      
        1 cacttcgagc acggccttgg cgcccggcgc cttgccttcc gtgtagccga agcccggatc
       61 gaagaagccg gcatagtgca cgcgatattc gccgatcagt ggatcgaacg gcgccatctc
      121 agcgacataa gcgggcggca cagagacgct ctccttcgag gcgaggatgt agaactcgtg
      181 cgggtcgagc acgagacggt ggtcgttgcc gagccgcacc tcttcccagt actgtccgac
      241 gtcgtaggcg ccgatctcgt cgacatcgat caccccggcg taacgccgcg cgcgatagcc
      301 gaccagaccc gatggcagga ccggcgcgag gctgatgctc aagtttagcc cgtcgcggat
      361 cgccgcctcg ccctcgacca gcccgacgtc ggaatggatc tcgcgcaaag cccggtcgcc
      421 aacccggccg ggctgctcgc cggcatcgag ctgctggccg acgcggcggc ggaacctgat
      481 ctgattgagc cgcgaaccgt agcgaacctt gatggagaag gtgcgcgggc aaatctcggt
      541 gaacagcggc cctgaatacc cttcctcgac gctgtcgaac ccgtcctggc ggtcggcgat
      601 cagccgcgtg aatacgtcga gacgccccgt cgagcttttc ggattggcga agccggcgat
      661 ggtcgagcgg agcttgagtt gctcgatcag cggcacgaga tagacgcagc ccgcttccag
      721 caccgcgccc ttggtgagat cgatctcgtg gaacttgagc gcgctgagct tgtcgcgcac
      781 cgtggcgttg gggccgggca ggaagctcgc ccggatgcga taggcgacct tgccgagcct
      841 gagatcgagg ctcgccggct ggatctggtt ggtttcgatc tctggcgcgc ccgcgatttc
      901 gccgccggca atcatcgcgc ggatggcctg gctcggaagg attccggtgc gggcgaacac
      961 gctgcgtggg ccgtccacgg ggtcggcaag cgcctcctcg ggaagcgcct cgaaaagcct
     1021 gtcgcgcggg gttgctggtt gcatgttggt ttctgtcacg ttgcgtgcct agcgtcagct
     1081 taagctcggc cttctccccc ggattcacaa gagggccgga accgccgggt ttgagccaag
     1141 ttcacccgag aatccaaagg cgctggcaca tgcttatagg acatcttgct ctcgtcgttg
     1201 ccgctctctt caccggtgcg gctatctacg tcaatttcgc cgagcagccg gcgcggcttc
     1261 agctggacga ccgcagcctg cttgccgagt ggaaaccttc ctatcagcgc ggcttcgcca
     1321 tgcaggcgag cctggcgatg cttggctttc tgcttggcgc cgtcgcctgg tggcagaccg
     1381 ggctcgcggc cttcctcctc ggcgcgctct atatcctggc gccttggccc tggacgcttc
     1441 tggtcatcaa gccggtcaac gacgcgctga tggcgaccga cctggcggcg gccggacccg
     1501 aaacccgcgc cctgatcgtc aaatggaacc ggctgcatgc cgtgcgcacc ctgctcggcg
     1561 ccgccgccgt catttccttt ctctttgccg tctccacggg ttgacttggg ccgagacttg
     1621 acggaacccg ttcagcgggg gcattaatgg ccctcgtggt gatttggccg gccggcttgc
     1681 agccacgtta aacaagtcgc taaaaggccg tgcgaggcga tcttcaagcc gatcccggtc
     1741 ttttggcggc agccagagtc cgggtttttt tatgcggccg gtcggaatga gatttcgaga
     1801 gtggagctga ccaatggccg caaacaagac tcgaacgaag ggcgcgagcg cctggcaacc
     1861 ggcgacgagg ctcgtgcatg aaggcacgct gcgcagccag ttcggcgaga cgtcggaagc
     1921 catcttcctc aactcgggct acatctatgc gagcgccgag caggccgaag cccgcttcaa
     1981 gggcgacgag gacggctacg tttacagccg ctacgccaac cccaccgtgt ccatgttcga
     2041 gacgcgcatg tgcgcgctgg aaggcgccga cgccgcgcgc ggcacgtcga gcggcatggc
     2101 ggccgtcgcc gcgtcgctgc tttgccatct gaagaccggc gatcacgtcg tctccgcccg
     2161 cgccctcttc ggcagctgtc gctacatcat cgaggatttg ctgccgcgct acggcatcga
     2221 gaccacgctg gtcgacgggc gcgatcttaa ggcttgggag aaagcggtca ggcccaacac
     2281 gcgcgccttc ttcttcgaga cgccgaccaa tcccgtgctc gagctggtgg atatcgaggc
     2341 cgttgcaaac atcgccaagc aggcgaaagc gctggtcgtg gtcgacaacg tgttcgccac
     2401 gcccctgctg caacggccgt tgcagcttgg cgccgacatc gtcgtttatt ccgccaccaa
     2461 gcatatcgac gggcagggac gctgcctcgg cggcgtggtg ctcgggccgc aagagttcat
     2521 cgaggagaag ctgcacaatt acctcaagca caccggcccc tcgctcagtc cgttcaatgc
     2581 ctgggtgctg ttgaaagggc tcgagacgct tccggttcgc gtcgagcggc acatcgcctc
     2641 ggccgcggcc atcgccgatt tcctcgcggc gcgcaaggac gtggcgcgcg tctactatcc
     2701 gggccgcgcc gatcatccgc aggccgcgct cgccaagcgg cagatggcgg cgggcggccc
     2761 gctggtcgcc ttcgaagtca agggcggcaa ggacgcggcc ttccgctgca tgaatgcgct
     2821 cgagatcatc aagatcagca acaatctcgg cgatgcgaag agtctcgtca ctcatcccgc
     2881 caccaccacg catcagcgcc tcgcgcccga gatacgggcg gaactcggca tcttcgacaa
     2941 cagcgtgcgc ctttcggtgg gactggagga cgccggcgac ttgatggccg atctcgacca
     3001 ggcgcttgcc gccgccaagt gagtacagcc ggtgccttgc tcacgcctgg cccctgtgcc
     3061 accttttgcg cgcgttttag cctgctaacg aggacgatca tgtgccggaa catcaagacg
     3121 ctgcataatt tccagccgcc cgccaccgac gaggaaatct atctgtcc
//
LOCUS       DATNAE010000288         3237 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2089815, whole genome shotgun sequence.
ACCESSION   DATNAE010000288 DATNAE010000000
VERSION     DATNAE010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3237
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2089815"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..1158)
                     /gene="folP"
                     /locus_tag="VN518_06485"
     CDS             complement(91..1158)
                     /gene="folP"
                     /locus_tag="VN518_06485"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HXE04256.1"
                     /translation="MADPKLYLRPIGFLYGETAAEAVAEGAALSLAGGPISFTAAELI
                     EGVPSCTERRLLAAKELAGSRDSDLARVLERVTTPRPPFVGLALFRPLIMGIVNVTPD
                     SFSDGGLYDKTEGAIVRAAELAAAGADIVDVGGESTRPGSDPAEEGDELGRVLPVLEG
                     LAGLNAAISIDTRKATVARAAARAGAKIFNDVSALTYDPDSLAAAADTGLSVILMHAK
                     GEPKTMHLDPRYEDVVLEVYDYLEGRIAAAEAAGIERARVAADPGIGFGKTMDHTLAL
                     LANLSLLHGLGVPILVGASRKRFIGGVAGGETPQSREPGSFAVALVASAQGAQILRVH
                     DVAGTRQALSVWRECVAGTGA"
     gene            complement(1323..3236)
                     /gene="ftsH"
                     /locus_tag="VN518_06490"
     CDS             complement(1323..3236)
                     /gene="ftsH"
                     /locus_tag="VN518_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006932090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HXE04257.1"
                     /translation="MWVFIGLLLVALFNLFQSPGVHVRGNEISFSQLLSDVDSGSVQD
                     VTIAGNQITGHYSDGRNFQTYAPNDPGLVDRLNKKGVKITAKPSEDDVPSLLGVLVSW
                     FPMLLLIAVWIFFMRQMQSGGGRAMGFGKSKAKLLTERHGRVTFDDVAGVDEAKEDLQ
                     EIVEFLRDPQKFQKLGGRIPRGALLVGPPGTGKTLLARAIAGEANVPFFTISGSDFVE
                     MFVGVGASRVRDMFDQAKKNAPCIIFIDEIDAVGRHRGAGLGGGNDEREQTLNQLLVE
                     MDGFEANEGIILIAATNRPDVLDPALLRPGRFDRQVVVPRPDIIGREKILKVHVRKVP
                     LAPDVDLRVISRGTPGFSGADLANLVNEAALLAARRSKRLVTQLEFEDAKDKVMMGAE
                     RRSMVMTEEEKTLTAYHEGGHALVALHQEASDPVHKATIIPRGRALGMVMRLPERDML
                     SYTRAKLKADITVAMGGRVAEELVFGYEKVTSGASSDIKMATLLARAMVTQYGMSDKL
                     GPLAYGDNEEEVFLGHSIARTQNMSDETQTIVDEEIHRLVDEGYEQARKILDEHIDDL
                     HLLARGLLEYETLSGDEIKDLLKGKPPVREFAADISPRPTPASAVPTAGRGKKAPPEP
                     EPDTGGMAPQPTS"
BASE COUNT          576 a         1060 c         1043 g          558 t
ORIGIN      
        1 caccaccgtt cacaaagtgt aagcaaaagt tacatcaatt ccgtttggtt aatcaggggt
       61 ttaacccctt ttttccacga cgcaagcgga ttaggcgccc gttccggcca cgcattcccg
      121 ccacacggaa agcgcctggc gcgttcccgc cacgtcatga acgcgcaaaa tctgggcacc
      181 ttgcgccgaa gccacaaggg cgacggcaaa ggaacctggc tcccgcgact gcggtgtttc
      241 gccgcccgcc accccgccga tgaagcgctt gcgcgaggct ccgacgagga tgggcacccc
      301 gagcccgtgc agcaggctga gattggcgag gagcgcgaga gtgtggtcca tggtcttgcc
      361 gaagccgatc cctgggtcgg cggcgacgcg ggcgcgctcg atgcctgccg cttcggccgc
      421 ggcgatgcgg ccctcgagat agtcgtagac ctcgagcacg acgtcttcat agcgggggtc
      481 gagatgcatg gtcttgggct cgcccttggc atgcatgagg atgacggaaa gcccggtgtc
      541 ggccgcggcg gcaaggctgt ccgggtcgta ggtcagcgcc gagacgtcgt tgaagatctt
      601 ggcgccggcg cgtgccgcag cccgcgccac ggtcgccttg cgcgtgtcga tggagattgc
      661 ggcgttgagc ccggcaagcc cttcgagcac cgggagcacg cgtccgagct cgtcaccctc
      721 ctccgccggg tctgagcccg ggcgggtcga ctcgcccccc acatcgacga tatcggcgcc
      781 agcggcagcc aactctgcgg cgcggacgat cgcgccttcc gtcttgtcgt agaggccgcc
      841 gtccgagaat gagtcaggcg tgacgttgac gatgcccatg atcagggggc gaaagagagc
      901 gagccccacg aagggcgggc gcggtgtggt cacacgctcg agcacgcggg caagatcgga
      961 gtcccgcgaa ccggcgagct cctttgcggc gaggagccgc cgctccgtgc agctcggcac
     1021 gccctcgatc agctctgccg cggtgaagga gatggggcct ccggccaagg agagtgccgc
     1081 gccttcagcc acagcttcgg ccgccgtctc gccgtaaagg aatccgatgg gcctgagata
     1141 gagcttcggg tctgccatgc cgcctgcctt agcacgctgc gccgacagcg ccacccctca
     1201 ctcgttatgc ccggccttgt agccggccat ccacgacttt gattcggtca acccaaaaac
     1261 gtggatgccc ggcacaaggc cgggcatgac aacgcttcaa ggaatgagag tcctaaagga
     1321 cctcagctcg tcggctgggg cgccatgccg ccagtgtcgg gctcgggctc aggcggcgcc
     1381 ttcttgccgc ggccggcggt ggggacggct gacgccggcg tcgggcgcgg cgagatatcg
     1441 gccgcgaatt cgcgcaccgg aggcttgccc ttgagcaagt ccttgatctc gtcgcctgac
     1501 agcgtctcgt attcgagcaa cccgcgcgca agcaaatgca gatcatcgat atgctcatcg
     1561 aggattttgc gcgcctgctc gtaaccctca tcgacgaggc ggtggatttc ctcatcgacg
     1621 atggtctgtg tctcgtcgga catgttctgg gtccgcgcga tggagtggcc taggaaaacc
     1681 tcctcctcgt tgtcgccgta ggcgagcggc ccgagcttgt ccgacatgcc atattgagtg
     1741 accatagcgc gcgccaacag cgtcgccatc ttgatgtccg aggaggcacc agaggtcacc
     1801 ttctcgtagc cgaacaccag ttcctccgcc acgcgtccgc ccatggcgac agtgatgtcg
     1861 gccttaagct tggcgcgcgt gtaggacagc atgtcacgct caggcaaccg catgaccatg
     1921 ccaagtgcgc ggccccgcgg gataatggtc gccttatgca ccggatccga cgcctcctga
     1981 tgcagagcca cgagagcatg gccgccctcg tgataggcgg tcagcgtctt ctcctcctcg
     2041 gtcatgacca tggagcggcg ctcagcgccc atcatgacct tgtccttggc gtcctcgaac
     2101 tcgagttgcg tgacgagccg cttcgaccgt cgcgcggcaa gcaacgccgc ctcgttcacg
     2161 agattggcga gatcggcgcc ggagaagccc ggcgtgccgc gggagatgac gcgcagatcc
     2221 acatcgggag cgagcggcac cttgcgcaca tgcactttca ggatcttctc gcgcccgata
     2281 atgtctggac gcggcaccac cacttggcgg tcgaaccggc ccggacgcag cagagcagga
     2341 tcgagcacgt ctggacggtt ggtcgcggcg atcaggatga tgccttcatt agcctcgaag
     2401 ccgtccatct cgaccagcag ctgattgagg gtctgctcgc gctcgtcgtt gccgccgcca
     2461 aggcctgcgc cacgatgacg gccgacggcg tcgatctcgt cgatgaagat gatgcaaggc
     2521 gcgttcttct tcgcctgatc gaacatgtcg cgcacgcgcg aagcgccgac gccgacgaac
     2581 atctcgacga agtccgagcc tgagatggtg aagaacggca cattggcttc gccggcgatg
     2641 gcgcgcgcga gcagcgtctt gccggtgccg ggaggaccta ccagcagagc gccgcgcggg
     2701 atgcgcccgc cgagcttctg aaacttctgc ggatcccgca ggaactcgac gatctcctgc
     2761 agatcctcct tcgcctcatc gacgccggcg acgtcatcga aggtgacgcg gccatggcgc
     2821 tcggtgagca gcttcgcctt ggacttgcca aagcccatgg cccggccgcc gccggactgc
     2881 atctggcgca tgaagaagat ccacacggcg atgagcagca gcatggggaa ccacgacacg
     2941 agcacgccga gcagcgacgg cacgtcgtcc tctgacggct tggcggtgat cttcacgccc
     3001 ttcttgttca gcctgtcgac gagaccggga tcgttcggcg cataggtctg gaaattccgg
     3061 ccatccgaat agtggccggt gatttgattg cccgcgatgg tcacatcctg cacgctgccg
     3121 ctatcgacgt cgctcaggag ctgcgagaag ctgatctcgt tgccgcgcac gtgcacgccc
     3181 gggctctgga acaggttgaa cagcgcgacc aggagcaggc cgatgaacac ccagatg
//
LOCUS       DATNAE010000289         2381 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_4187448, whole genome shotgun sequence.
ACCESSION   DATNAE010000289 DATNAE010000000
VERSION     DATNAE010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4187448"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..1444
                     /locus_tag="VN518_06495"
     CDS             77..1444
                     /locus_tag="VN518_06495"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXE04258.1"
                     /translation="MSDQAEPSARTLEPGLLARTGNAVMRPFREIGARLKAALWLIVA
                     VGVIMASLVLIAGLSKAFALIGFVSFVASVVLLPRHDGDQALPGALDRPAAVSRTTSA
                     MHLMADALPDPVIVLNPAGHVLFCNAPARGLFASLREGSHISSVIRTPEFLDAVSAAP
                     ERGRAVTVTYAERVPVGRRMAATVAPLARGSERGGNILVLLRDLTEGERINQMRADFI
                     ANASHELRTPLASLRGFIETLQGTAKEDTGARERFLPIMAEQASRMTRLIDALLSLSR
                     VEMNAHVPPSGLVDLNDVLNSTKDTLEPLARETGFRLEVARFPRPAIVRGDRDELLQV
                     MQNLVQNAFKYGERGGQVRIEAKHIPSLGRQAGRYAIAVVDNGLGIAPEHLPRLTERF
                     YRVDVASSREKGGTGLGLAIVKHILNRHRGELAIASKPGKGSTFTVMLNEARASQADK
                     GAAAG"
     gene            1638..>2381
                     /gene="pstS"
                     /locus_tag="VN518_06500"
     CDS             1638..>2381
                     /gene="pstS"
                     /locus_tag="VN518_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HXE04259.1"
                     /translation="MRVMSYAAAVGLAAAFTISTAQAADISGAGATFPYPIYAKWADA
                     YKKETGIGLNYQSIGSGGGIKQIEAKTVTFGATDKPLGEKDLTDNGLVQFPMVMGGIV
                     PVINVEGVKAGEIVLDGPTLAKIFLGEVAKWDDEAIKKLNPKAKLPSQAIAVVHRSDG
                     SGTTFNFTNYLNKVSASWKDKVGSDASVVWPVGIGAKGNEGVANNVAQTKGAIGYVEY
                     AYAKQNKMPYANLINKDGKSVSPELKSFQA"
BASE COUNT          431 a          782 c          772 g          396 t
ORIGIN      
        1 caaaagcgtg ttaatgcagg ggcaattata gtggcgagcc gcttcgcgat tcgtgcggac
       61 gggcggggag aagccattga gcgatcaagc agaaccgagc gcgcggacgc ttgagcccgg
      121 tctcctggcg cggaccggca acgccgtcat gcggcccttc cgcgagatcg gggcgcggct
      181 caaggcggcc ttgtggctga tcgtcgccgt cggcgtcatc atggcgtcgc tggtgctgat
      241 cgcgggtctc tccaaggcct tcgccctgat cggcttcgtc agtttcgtcg cctccgtggt
      301 gctgctgccc cgccacgacg gcgatcaggc gcttcccggc gcgctcgacc gccccgccgc
      361 cgtttcgcgc acgacctcgg ccatgcattt gatggccgat gcgctgcccg acccggtgat
      421 cgtgctcaat cccgcgggcc atgtgctgtt ctgcaatgct ccggcgcgcg gcctgttcgc
      481 ctccctgcgc gaaggaagtc acatctcgtc cgtgatcagg acgccggagt tcctcgatgc
      541 cgtctcagcc gcgccggagc gtggacgcgc cgtcaccgtc acctatgccg agcgcgtgcc
      601 ggtcggccgg cgcatggcgg cgaccgtggc gccgctcgcg cgcggcagcg agcgcggcgg
      661 caatatcctc gtcctgctgc gcgacctcac cgagggcgag cgcatcaacc agatgcgcgc
      721 cgacttcatt gccaatgcga gccacgagct caggacgccg ctcgcctcgc tgcgcggctt
      781 catcgaaacg ctgcaaggca ccgccaagga ggacacgggc gcgcgggagc ggttcctgcc
      841 gatcatggcc gagcaggcct cgcgcatgac gcggctgatc gatgcgctgc tgtcgttgag
      901 ccgggtcgag atgaacgcgc atgtgccgcc gtccggcctc gtcgatctca acgacgtgct
      961 gaatagcacc aaggacacgc tcgagccatt ggcgcgcgaa acgggcttcc gcctcgaggt
     1021 ggcgcgcttt ccgcggccgg cgatcgtgcg cggcgaccgc gacgagctgc tccaggtgat
     1081 gcagaatttg gtgcagaacg ccttcaaata tggcgagcgg gggggtcagg tgcggatcga
     1141 ggcgaagcac atcccttcgc tcgggcgcca ggccggccgc tacgccatcg ccgtggtcga
     1201 caacgggctc ggcatcgcgc ccgagcatct gccgcggctg accgagcgct tctaccgggt
     1261 cgacgtggcg tcgagccggg agaaaggcgg caccggtctt ggactcgcca tcgtcaagca
     1321 catcctcaac cgccacaggg gcgagctagc catcgcgtca aagccgggca agggctcaac
     1381 ctttacggtc atgctcaacg aggcgcgcgc gagccaggcg gacaagggcg ccgctgcggg
     1441 ctagcgcctt ctttccacaa tcttgggctg ggaaacccgg ccaatcccgg gcttctccgg
     1501 cactgtcacc caactgtcat ataactgaca tagtagcttt attacccggc ggtaacgctc
     1561 gcccggaggt cggggaaggg tccccggccc gtatggtcct tgttggaccg ttatctccat
     1621 ccctaaggga gagcgaaatg agggttatgt cttatgcggc agccgtaggc ctcgccgcag
     1681 cgttcacaat tagcacggcc caggcagccg atatttcggg tgccggcgcc accttcccct
     1741 atccgatcta tgcgaaatgg gcggacgcct acaagaagga gacgggcatc ggtctcaact
     1801 atcagtcgat cggctccggt ggtggcatca agcagatcga agccaagacg gtgaccttcg
     1861 gcgccaccga caagccgctc ggcgagaagg atctgactga caacgggctg gtccagttcc
     1921 ccatggtcat gggcggtatc gttcccgtca tcaatgtcga aggcgtcaag gccggcgaga
     1981 tcgtgctcga cgggccgacg ctggccaaaa tcttcctcgg cgaggtcgcc aaatgggacg
     2041 atgaggcgat caagaagctc aatccgaaag ccaagcttcc ctcacaggcg atcgccgtcg
     2101 tgcaccgctc ggacggatcg ggcaccacct tcaacttcac caactacctg aacaaagtaa
     2161 gcgcgtcttg gaaggacaaa gtcgggtccg acgcctcggt cgtgtggccg gtcggcatcg
     2221 gcgccaaggg caacgagggc gtcgccaaca acgtggccca gaccaagggc gccatcggct
     2281 atgtcgagta cgcctacgcc aagcagaaca agatgccgta cgccaacctg atcaacaagg
     2341 acggcaagtc ggtatcgccc gagctgaaga gcttccaggc g
//
LOCUS       DATNAE010000290         2830 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_284401, whole genome shotgun sequence.
ACCESSION   DATNAE010000290 DATNAE010000000
VERSION     DATNAE010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284401"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /gene="uppS"
                     /locus_tag="VN518_06505"
     CDS             complement(<1..537)
                     /gene="uppS"
                     /locus_tag="VN518_06505"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HXE04260.1"
                     /translation="MTLAVDKLAWQSADRPAVRHVAIIMDGNGRWAAERGLPRAEGHR
                     QGVESVRRTVEAALDLGITHLTLFSFSSENWSRPKHEISDLFGLLRRFIRRDLADLHK
                     NGVKIRVIGTRSGLDADLLRMLGDAVELTKDNVALNLTIAFNYGARDEIARAARRIAE
                     DVAKGALAPAEVTEARLGG"
     gene            complement(589..1146)
                     /gene="frr"
                     /locus_tag="VN518_06510"
     CDS             complement(589..1146)
                     /gene="frr"
                     /locus_tag="VN518_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HXE04261.1"
                     /translation="MSSFDLKEIERRMRGALGVLKQEFGGLRTGRANASLLDPIMVTA
                     YGNPMALNQLATINVPEPRLITVQVWDRSQVGAVERAIRESDLGLNPVVEGQLMRLPI
                     PELNQERRQEIAKVAHKYAEQARIAVRNVRRDGMEHLKKAEKDHEMGKDEHHSLSTKV
                     QELTDKIIKEIDEALASKEAEIMQV"
     gene            complement(1212..1958)
                     /gene="pyrH"
                     /locus_tag="VN518_06515"
     CDS             complement(1212..1958)
                     /gene="pyrH"
                     /locus_tag="VN518_06515"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018391196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HXE04262.1"
                     /translation="MAKPQAAATLRYRRILLKLSGEALAGDKGYGIDLGVIAGIARDV
                     VAAAASGAEICLVIGGGNIFRGLRADEVGIDRARADYMGMLATVMNALALQTAIEHAG
                     KPARVLSAIPMPTICESYIRDKALDHLAKGRVVIFAGGTGNPFFTTDTAAALRAAEMS
                     CEALFKATQVDGVYSADPQKTPNARRYETLSYDDVLKQDLRIMDGAAIALARDNRIPI
                     IVFSIKSQGGLALVLQGKGSSTTIDGGKGA"
     gene            complement(2031..>2830)
                     /gene="tsf"
                     /locus_tag="VN518_06520"
     CDS             complement(2031..>2830)
                     /gene="tsf"
                     /locus_tag="VN518_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008546834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HXE04263.1"
                     /translation="GLAKAAKKAGRIAAEGLIGLAADAKQAVLVEVNSETDFVARNQK
                     FQDMVSGIAAAALKAKGDVDKLAAAKYGSGKQTVADTIKEMVGSIGENMTLRRAAYLA
                     AKDGTVASYMHNAIAPGLGKIGVIVALESKGNPEDVTAFGRQVAMHIAFANPQSLDVD
                     GLDKTVIERERAVLTEQAKESGKPPQVIEKMVEGRLRKFYEEVVLLAQPFVHDSEQTV
                     AKAMEAAGKTAGAPIKITGFHRFALGEGIDRAETDFAAEVAAAVSQS"
BASE COUNT          445 a          933 c          888 g          564 t
ORIGIN      
        1 gcccccgagc cgcgcctcag tcacctcggc gggagcgagg gcgcccttcg ccacgtcctc
       61 ggcgatgcgg cgcgcggcgc gggcgatctc gtcgcgggca ccgtaattga aggcgatggt
      121 gagattgagc gcgacgttgt ccttggtgag ctcgacggcg tcgccgagca tcctcaacag
      181 gtcggcatcg agtcccgagc gcgtgccgat cacgcggatc ttcacgccgt tcttatgcaa
      241 atcggcgaga tcgcggcgga tgaagcggcg caacagaccg aacagatcgc tgatctcatg
      301 cttgggccgg gaccagtttt ccgacgagaa gctgaacaag gtgagatggg tgatgccgag
      361 gtcgagcgcc gcctcgaccg tgcgccgcac gctttccacg ccctgacggt ggccttcggc
      421 gcggggcagg ccgcgctcgg cggcccagcg tccattgccg tccatgatga tcgcaacatg
      481 gcgcaccgcc ggtctgtcgg ccgattgcca agcaagctta tccaccgcaa gcgtcacgtt
      541 cggctgctcc gggttagggg taagggcgct tcctctgatc ttgcgcaatt agacctgcat
      601 aatctcggct tccttgctgg caagggcctc gtcgatttcc ttgatgatct tatcggttaa
      661 ctcttgcacc ttggtggaaa gcgagtggtg ctcgtccttg cccatctcgt gatctttctc
      721 ggccttcttc agatgctcca tgccgtcccg gcgcacattg cgcacggcga tgcgcgcctg
      781 ctcggcatat ttatgggcga ccttggcgat ctcctgccgc cgctcctggt tcagttcggg
      841 gatcggtagg cgcatcagct gtccctcgac caccgggttg agccccaaat ccgactccct
      901 gatggcgcgc tcgacggcgc cgacctggct gcggtcccag acctgcacgg taatgagtct
      961 tggctcagga acgttgatgg tggcgagctg attgagcgcc atggggttgc cgtaggccgt
     1021 gaccatgatc gggtccagca ggctcgcatt ggcgcggccg gtgcgcaatc caccgaattc
     1081 ctgcttcagc acgccgaggg cgccacgcat ccgccgctcg atctccttaa ggtcgaagct
     1141 cgacatgtcg ccttccctgc tccgccgggc gcgcccctct acagcgcaac tcggccgaac
     1201 ctccgcgttt cctaggctcc cttgccgcca tctatcgtgg tcgaggagcc ctttccctgc
     1261 aatacaagag ccaagccgcc ttggctcttg atggaaaaca caattatcgg aatccgattg
     1321 tctcgggcaa gggcgattgc ggcaccgtcc atgatgcgca gatcttgctt aaggacatcg
     1381 tcatagctga gtgtctcata gcggcgggcg tttggcgtct tttgcgggtc ggcggaatag
     1441 acgccgtcaa cctgggtggc cttgaacagc gcttcgcagc tcatctccgc cgcacgtagt
     1501 gcggctgccg tgtccgtggt gaagaacggg ttgccggtgc ctccggcgaa gatcaccacc
     1561 cggcccttgg ccagatgatc gagggccttg tcgcggatat aggattcgca aatggtgggc
     1621 atcgggatgg ccgagagcac ccgcgccggc ttgcccgcgt gctcgatcgc cgtctgcagc
     1681 gcgagcgcgt tcatcaccgt cgccagcatg cccatatagt cggcgcgcgc ccggtcgatt
     1741 ccgacctcgt cggcccttag cccacgaaag atgttgccgc cgccgatcac caggcaaatc
     1801 tcggcgcccg aagcggcagc cgcgacgaca tcgcgggcga tcccggcaat cacgccgagg
     1861 tcgatgccgt agcccttgtc gccggcgagc gcttcgcctg acaatttgag caggattcgc
     1921 cgatagcgaa gtgttgcggc cgcttgaggc tttgccatca gctcaaactc cggcgcgagt
     1981 ggcgggttgg cgagcgcgcg tggacgtctg cccccgcacg ccgccccacc ctaagattgc
     2041 gagaccgccg cggcaacctc ggccgcaaaa tcggtttccg cgcggtcgat cccctcgccc
     2101 aaggcaaacc ggtggaaacc tgtgatcttg atcggtgcgc cggccgtctt gccggcggcc
     2161 tccatggcct tggccacggt ctgctcggaa tcgtgcacga agggctgtgc cagaagcacg
     2221 acctcctcgt agaatttccg caggcggccc tcgaccatct tctcaatgac ttgcggtggc
     2281 ttgcccgact ccttggcctg ctcggtcagc accgcccgct cgcgctcgat aacggtcttg
     2341 tccaaaccgt cgacatcgag gctttgcggg ttcgcgaaag cgatatgcat ggcgacctga
     2401 cggccgaagg ctgtgacgtc ctccgggttg cctttcgact cgagcgcgac gatgacgccg
     2461 atcttgccga ggccaggcgc aatcgcgttg tgcatgtagc tggcgacggt gccgtccttg
     2521 gcggcgagat acgcggcgcg gcgaagcgtc atgttctcgc cgatcgagcc gaccatctcc
     2581 ttgatggtgt cggcgaccgt ctgtttgccg ctgccgtatt tcgccgcggc cagcttgtcg
     2641 acatcgccct tggccttgag ggccgcagcg gcgatgccgc tcaccatgtc ctggaacttc
     2701 tggttgcggg cgacgaagtc cgtctcggag ttgacctcga ccagcaccgc ctgtttcgcg
     2761 tcggcggcaa gaccgatcaa accttccgcg gcgatgcggc cggccttctt ggcggccttg
     2821 gcgaggccct
//
LOCUS       DATNAE010000291         4698 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_888197, whole genome shotgun sequence.
ACCESSION   DATNAE010000291 DATNAE010000000
VERSION     DATNAE010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4698
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_888197"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..165)
                     /locus_tag="VN518_06525"
     CDS             complement(<1..165)
                     /locus_tag="VN518_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011982392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HXE04264.1"
                     /translation="MRDNAEVRLNERLAAEENRTLLRFLTCGSVDDGKSTLIGRLLFD
                     SAQIYEDQLSS"
     gene            complement(192..1118)
                     /gene="cysD"
                     /locus_tag="VN518_06530"
                     /pseudo
     CDS             complement(192..1118)
                     /gene="cysD"
                     /locus_tag="VN518_06530"
                     /inference="COORDINATES: protein motif:HMM:NF009214.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
     gene            1323..2579
                     /locus_tag="VN518_06535"
     CDS             1323..2579
                     /locus_tag="VN518_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014891873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HXE04265.1"
                     /translation="MRVGIIGLGYVGLPLAVYMARHFPVLGYDVNTARIGELAGGRDR
                     TGEVTDEEFAAARTISFTADIEALRDCNFYIVTVPTPIDQAKRPDLTALMRASEAVGR
                     VLTPGDVVVYESTVYPGATEEVCVPILAKASGLTFNRDFFVGYSPERINPGDLQHRLP
                     TIVKVTSGSTPEAADLVDKVYGSVVTAGTHKVSSVRVAEAAKVIENIQRDVNIALINE
                     LAMLFKDLGLDTSEVLAAAGTKWNFHAYRPGLVGGHCIGVDPYYLTHKAQSIGFHPEM
                     ILAGRRINDGMASFVARDIIKAMLHRKLKMDDAKVLVMGFTFKENVPDTRNTKVVDLV
                     RELGSFLDVVVYDPVADREDALHEYGVGIVDKLPDDFFQAVVLAVKHREIAGLGRDRL
                     GALLAPGGLIYDITGVLAPGERDAHI"
     gene            complement(2576..3442)
                     /gene="pssA"
                     /locus_tag="VN518_06540"
     CDS             complement(2576..3442)
                     /gene="pssA"
                     /locus_tag="VN518_06540"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HXE04266.1"
                     /translation="MFPPFDPEKDDRKPRARVFARGQVPTRVLIPNIFTLLGLCAGLT
                     AIRMAIEHRWDLAVAALVFAAFLDGIDGRVARLLKAQSRFGAELDSLADFVNFGVAPA
                     IIMFNWALDDLKSTGWIAVLIYAVCAALRLARFNVALDRTDLPAWKSSYFVGVPAPAG
                     AIVLLLPIYAQDLGLHLPTLTPLVLLYTLAVALLMVSRVPTFSGKLIGQKIAREYVLP
                     VFVLVALFIACLLTYPSLTLAAGSVIYLALIPVSAYRYFQVERKMELIAKGQNGKGAS
                     EPTAIEPPSFPR"
     gene            complement(3467..4171)
                     /locus_tag="VN518_06545"
     CDS             complement(3467..4171)
                     /locus_tag="VN518_06545"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HXE04267.1"
                     /translation="MSERHTILDSIRNAFVPIHPEGRKFIAGFAVAALILFLLAPPLG
                     WLGVIATVLCAYFFRDPERVTPQRPGLVVSAADGKLVNIGTVVPPKELALGEALLTRI
                     SVFLSVLDVHVVRAPVAGRVVHSAYVPGKFLNAELDKASEENERRAMVIETPAGEKIG
                     VVLIAGLIARRIVTFVGEGASVAAGERIGLIRFGSRVDVYLPANGDVLVAAGQTAIGG
                     ETALAELPDVVRASGP"
     gene            complement(4188..>4698)
                     /locus_tag="VN518_06550"
     CDS             complement(4188..>4698)
                     /locus_tag="VN518_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011314524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXE04268.1"
                     /translation="DAQVREAARLAQIHDFIMTLPQGYVSLVGERGLKLSGGEKQRVA
                     IARTILKSPPILMLDEATSALDSHTEKEIQDALERVARERTSLVIAHRLSTVVHADNI
                     IVLDHGEIVEQGTHLELLARGGRYASLWAHQREADQARERLAQVLDHEPEPESRAAER
                     LEDEALAGN"
BASE COUNT          877 a         1518 c         1486 g          817 t
ORIGIN      
        1 gctgctcaac tgatcctcat agatctgcgc cgagtcgaag aggagccggc cgatcagcgt
       61 gctctttccg tcatcgacgc tgccgcaggt gaggaagcgc aacagggtgc gattctcctc
      121 agcggcgagg cgctcattca gccgtacctc ggcattgtcc cgcatgctgt ccaccacact
      181 caaccgcggc atcagaaata cccttcctgc ttcttcttct ccatcgagcc ggcctgatcg
      241 tggtcgatga cccggccctg gcgctccgac gagcgcgccg cgaccagctc ctcgatgatc
      301 tcgggcacgg tcgcggcatt cgattttatg gcgcctgaca ggggatagca gccaagcgtg
      361 cggaagcgca cgcgccgcat cgtgggcttc tcgccttcat ccatgggcag gcgatcgtca
      421 tcgaccatga tcagcatgcc gccacgttcg accaccgggc gctcggcggc gaaatagagc
      481 ggcaccaccg gaatgtcctc gagatagatg taggtccaca cgtcgagctc ggtccaattc
      541 gacagcggaa acacgcgcat gctttcgccg gatttgatct gggtgttggc gaggtgccag
      601 agttcggggc gctgccgctt cgggtcccaa ttgtgggcgg cgttgcggaa ggagaacaca
      661 cgctccttgg cgcgcgactt ttcctcatcg cgccgcgccc cgccgaaggc cgcatcgaaa
      721 tgatgctgct cgagcgcctg cttgaggctc tgcgtcttca tgatgtcggt atagagcgcg
      781 ctgccatggg taatcggcga gatgccgtcg tgcacgccct gctcgttgac atgcaccatg
      841 agatcgaccc cgagccgctt cgcctcgcgg tcgcggaagg cgatcatctc gcgaaacttc
      901 caggtggtat cgacatggag gagagggaat ggcggcttcg ccggatagaa cgccttcatg
      961 gcgagatgca ccatgaccga ggagtccttg ccgatcgaat agagcatgac ggggtggtgg
     1021 aattccgcca ccacctcacg catgatgtgg atgctctcag cctcgagctg gcgcagatag
     1081 gttgaaagtc tatgcttcat gcgcgtgccg ccctgccatt tttcggcttt ggccttagca
     1141 tcttagcggg ccgaggacaa attcggaccc tcgcggccgc tctctgaact ttgcgaaaaa
     1201 ctgcgcgaaa tgctaaacca cgccccgata tcgagggacg atagaacctg cccatgaatg
     1261 accaaattaa ggcggcagcg cgtgagggat ccgggcaagc gaggccgccc gcgcttgaga
     1321 acgtgcgggt cgggatcatc gggcttggct atgtcgggct gccgcttgcc gtttacatgg
     1381 cgcgtcactt ccccgttctt ggctatgacg tcaatacggc ccgcatcggc gagcttgccg
     1441 gcggccgcga ccgcaccggc gaggtcaccg acgaggaatt cgccgccgcc cgcaccattt
     1501 ccttcactgc cgatatcgag gctctgcgcg actgcaattt ctacatcgtc acggtgccga
     1561 cgcctatcga tcaggccaag cgtcctgacc tcacggcgct gatgcgggcg agcgaggcgg
     1621 tcggccgcgt gctcacgcct ggcgacgtgg tggtctacga atcgacggtc tacccgggcg
     1681 ccacggaaga ggtttgcgtg ccgatcctgg cgaaggcgtc tgggctcacc ttcaaccgcg
     1741 acttcttcgt tggctattca cccgagcgca tcaatcccgg cgatttgcag caccggctcc
     1801 ccaccatcgt caaggtcacc tcgggctcga cgccggaagc cgccgacctc gtcgacaagg
     1861 tctatggcag cgtggtgacc gcgggcaccc acaaggtgag ctcggtcaga gtggcggaag
     1921 ccgccaaggt catcgagaac attcagcgcg acgtgaacat cgcgctgatc aacgagcttg
     1981 ccatgctgtt caaggatctt gggctcgaca ccagcgaggt gctcgccgcc gccggcacca
     2041 aatggaattt tcacgcctat cgtcccggcc tcgtcggcgg ccactgcatc ggcgtcgatc
     2101 cctattatct gacccacaag gcgcaatcga tcggctttca cccggagatg atcctcgccg
     2161 ggcgccgcat caatgacggc atggcgagct tcgtcgcccg cgacatcatc aaggccatgc
     2221 ttcaccgcaa gctgaagatg gacgacgcca aggtcttggt catgggcttc accttcaagg
     2281 aaaacgtgcc cgacacgcgc aacaccaagg tcgtcgacct cgtgcgcgag cttggcagct
     2341 tcctcgatgt ggtggtctac gatcctgtcg ccgaccgcga ggacgcgctg cacgaatatg
     2401 gcgtcggcat cgtcgacaaa ctgccggacg atttcttcca ggcggtggtg ctcgccgtga
     2461 agcatcgtga gatcgcgggg ctcgggcgcg atcgtctagg cgccctgctc gccccaggcg
     2521 ggctgatcta cgacatcacc ggtgtgctgg cgcccggcga acgcgacgcg cacatctagc
     2581 gcgggaagga cggcggctcg atcgccgtgg gctcggaagc gcccttgcca ttctgcccct
     2641 tggcaatcag ctccatcttc cgctcgacct ggaaatagcg ataggcgctc accgggatga
     2701 gcgcgagata gatgaccgag cccgcggcaa gcgtcagcga cggataggtc agcaggcagg
     2761 cgatgaagag cgcgacgagc acgaacaccg gaagcacgta ctcgcgtgcg atcttctggc
     2821 cgatcagctt gcccgagaaa gtcggcacgc ggctcaccat cagaagcgcg accgcgagcg
     2881 tatagagcag cacgagcggg gtgagggtcg gcaaatgcag gccgagatcc tgcgcgtaga
     2941 tcggcaatag gagcacgatg gcgccggccg gcgccggcac gccgacaaaa tagctgcttt
     3001 tccacgccgg cagatctgtc cggtcgagcg ccacattgaa gcgcgcaagc ctgagcgcgg
     3061 cgcacactgc atagatcaga acagcgatcc agcccgtgct cttcagatcg tcgagcgccc
     3121 agttgaacat gatgatggcc ggcgccacgc cgaaattgac gaaatcggcg agcgaatcga
     3181 gctcggcgcc gaagcgcgat tgggccttga gcagccgcgc gacgcgccca tcgatgccgt
     3241 cgagaaaggc ggcgaagacg agcgcggcga cggccaggtc ccagcgatgc tcgatggcca
     3301 tgcggatggc agtcaacccg gcgcacagcc cgagcaaagt gaagatgttg gggatcagga
     3361 cccgcgtcgg cacttggccg cgcgcgaaca cccgcgcccg cggcttgcgg tcgtcttttt
     3421 caggatcgaa gggaggaaac atgggacgaa agcctctccg ccggcctcaa ggcccgctgg
     3481 cgcgaactac atccggcagc tcggccaagg ccgtctcgcc gccgatcgcc gtctggccgg
     3541 ctgcaaccag aacgtcgcca ttcgccggca gataaacatc gacccggctg ccgaaacgga
     3601 tcagcccgat acgctcgcct gccgctacgc tagccccctc gcccacaaaa gtcacgatcc
     3661 gccgagcgat caggcctgcg atcagcacca cgccgatctt ctcccctgcc ggagtttcga
     3721 tgaccatggc gcggcgctca ttctcctcgc tcgccttgtc gagctcggca ttcaggaact
     3781 tccccggcac gtaggcacta tgcaccacgc gcccagcaac gggcgcccgc accacatgta
     3841 cgtcgagcac cgagagaaac accgagatgc gggtgaggag cgcctcgccg agcgcaagct
     3901 ccttgggcgg aacgacagtg ccgatattga cgagcttgcc gtcggcagcg ctcaccacga
     3961 ggcctgggcg ctgcggcgtt accctctccg gatcgcggaa gaaataggcg cagagcacag
     4021 tggcgatcac gccgagccag ccgagcggcg gcgcaagcag gaacaggatc agcgccgcca
     4081 cggcaaagcc ggcaatgaat ttgcggcctt cggggtggat cggcacgaag gcgtttctga
     4141 tgctgtcgag gatggtgtgg cgttcgctca tgggtcaggt catggtccta gttgccggca
     4201 agagcctcgt cttcgagccg ctctgcagcg cgcgactctg gctcgggttc gtggtcgagc
     4261 acctgcgcga ggcgctcgcg ggcctggtct gcctcgcgct ggtgcgccca caggcttgca
     4321 taacgtccgc cgcgcgcaag cagctcaaga tgggtgccct gctcgacgat ctcgccatgg
     4381 tcgagcacga tgatgttgtc ggcatgcacc acggtggaga gccggtgggc gatgacgagc
     4441 gacgtgcgct cacgcgcgac cctttccagc gcatcctgga tctccttctc ggtgtggctg
     4501 tcgagcgccg atgtcgcctc gtccaacatt aggatcggcg gggattttag gatggtgcgg
     4561 gcaatggcca cgcgctgctt ctcgccgccg gaaagcttca gcccgcgctc gccgaccagc
     4621 gaaacgtagc cttgcggcag ggtcatgatg aagtcgtgga tctgggcgag ccgcgccgcc
     4681 tcgcgcactt gcgcgtcg
//
LOCUS       DATNAE010000292         2351 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_586780, whole genome shotgun sequence.
ACCESSION   DATNAE010000292 DATNAE010000000
VERSION     DATNAE010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586780"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="VN518_06555"
     CDS             complement(<1..771)
                     /locus_tag="VN518_06555"
                     /inference="COORDINATES: protein motif:HMM:NF014766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HXE04269.1"
                     /translation="MKIKRVEPIAVSLPMKKPVVMAGVEIARADNVLVRIEANDGLVG
                     WGEAASAPTMTGETVESMTAAIAYLASALEGRPADDIAGALAAMAARMYANHGAKAAI
                     EIALHDLVGRATNQPAYALLGGKQRSRMAVRGVIGTGALASDLLEAEKKKADGYIAFK
                     IKVGIDAPLIDGERTRRLCRLLGHDALISSDANQGWSAEQAAQYVRAVADAGLDFFEQ
                     PVSADDLAGMTAVAAAAGKIAIGADEGIHSLDDIRRHHE"
     gene            complement(829..2025)
                     /locus_tag="VN518_06560"
     CDS             complement(829..2025)
                     /locus_tag="VN518_06560"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HXE04270.1"
                     /translation="MSENGPLAGIRVLDLTRILAGPLCTMMLGDMGADVIKVEPPETG
                     DDTRAWGPPFLAGDAVYFLGVNRNKRSLTLNMGVPEGQKVLAGLIEKADVLIDNFRLG
                     TLEKWGFADAWFERHAPRLVRCSITGYGSSGPKAALPGYDFILQAESGLMSICGEPDG
                     RPTKYGVAIVDVCTGMLASNSILAALHARERTGKGQKVELSLYETSLAMLVNVAASYL
                     AAGRNAGRFGNGHPSIVPYTTYQTADGMIALGVGNERQFARVAEVLGHPEWAKDAQFT
                     SNRARVENRSVIDGLINAALSHDKADAWLAKLKAAGIPCGKINSVAEALDDAQTAARR
                     MIETIEHPATGALKMLGIPFKFSATACSVRRPPPTLGQHSEEILAGELGLDEKAIAEL
                     RRAKVV"
     gene            complement(2029..>2351)
                     /locus_tag="VN518_06565"
     CDS             complement(2029..>2351)
                     /locus_tag="VN518_06565"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HXE04271.1"
                     /translation="CVPSKDLLKETLAWCETIKGHSPQTLRMTKKSLNFESDLLYASW
                     QHGMELLAHVWGSEESLEGMDAFLEGRKPDFQKFRMRNKRELEDYLDGCAKDLNAPPS
                     MRRK"
BASE COUNT          370 a          762 c          771 g          448 t
ORIGIN      
        1 ctcatggtgg cggcggatgt cgtcgaggga atggatgccc tcgtcggcgc cgatcgcgat
       61 ctttcctgct gcggcggcca ccgctgtcat gccggcgagg tcgtctgccg acacgggctg
      121 ctcgaagaaa tccaatccgg catcggcaac cgcgcgcacg tattgcgccg cttgctccgc
      181 gctccagccc tggttggcgt cggatgaaat cagcgcatcg tgcccgagca accggcacag
      241 acggcgggtg cgttcgccgt caatgagcgg cgcgtcgatg ccgaccttga tcttgaacgc
      301 gatgtagccg tctgccttct tcttttccgc ctcgagcagg tcggaggcga gcgcgccggt
      361 gccgatgacg ccccgcacgg ccatgcggct tctctgcttg ccgccgagca aggcgtatgc
      421 cggctggttg gtggcgcggc cgacgagatc gtgcagcgcg atctcgatcg cggccttcgc
      481 gccgtggttg gcatacatgc gcgcagccat ggcggcgagc gcgccggcga tgtcatcggc
      541 cggccggccc tcgagcgccg acgcgaggta ggcaatcgcc gccgtcatgc tttcgaccgt
      601 ctcgccggtc atggtcggcg cggaagcggc ctcgccccag ccgacgaggc cgtcattcgc
      661 ttcgatgcgc acaagcacgt tgtccgcccg ggcaatctcg acgcccgcca tgaccacggg
      721 ctttttcatc ggcaggctga ccgcgatcgg ctcgacgcgc ttgatcttca tggggcttcc
      781 tcagtgctgc tcgggctcga cgcgcctccg gtgatcatgg gcaagccctt agacgacctt
      841 ggcccgacgc agttcggcaa tggccttctc atcgaggccg agctcgccgg caaggatttc
      901 ctcgctgtgc tgcccgagcg tcggcggcgg ccgccgcacg gagcaggcgg tggcggagaa
      961 cttgaatgga atcccgagca tcttcaaggc gccggtggcg ggatgctcga tggtctcgat
     1021 catccggcgc gcggccgtct gcgcatcgtc aagcgcttct gcgactgaat tgatcttgcc
     1081 gcaaggaatt cccgccgctt tgagcttggc gagccaagcg tcggccttat cgtgggacaa
     1141 cgccgcgttg atcaggccgt cgatcaccga gcggttttcg actcgcgcac ggttgctggt
     1201 aaattgcgcg tccttggccc attcggggtg accgagcacc tcggcgacgc gcgcgaactg
     1261 gcgctcgttt cctaccccga gcgcgatcat gccgtcggcg gtctgatagg tcgtatacgg
     1321 cacgatgctg ggatggccgt tgccgaagcg cccggcattg cggccggcgg cgagatagct
     1381 cgctgccacg ttgacgagca tcgccagcga cgtttcgtaa agcgagagct cgaccttctg
     1441 gcccttgccg gtgcgttcgc gcgcatggag cgccgcgaga atcgagttcg atgcgagcat
     1501 accggtgcac acgtcgacga tcgcgacgcc gtatttcgtc ggcctgccgt cgggctcgcc
     1561 gcaaatactc atgagcccgg attccgcctg caggatgaag tcatagccgg gaagcgccgc
     1621 cttggggccg ctcgatccgt atcctgtgat ggagcaacgc accagccgtg gtgcgtgccg
     1681 ctcgaaccaa gcgtctgcaa aaccccactt ctcgagcgtg cccaagcgga agttgtcgat
     1741 gagcacgtcg gccttctcga tcaggccggc gagaaccttc tgcccctccg gcacccccat
     1801 gttgagcgtg agcgaacgct tgttgcggtt gacgccgagg aaatagacgg cgtcgccggc
     1861 gagaaagggc ggcccccagg cgcgggtatc gtcgccggtc tcgggcggct cgaccttgat
     1921 gacgtcggcg cccatgtcgc cgagcatcat cgtgcacaac ggccctgcca ggatgcgggt
     1981 gagatcgagc acgcggatcc cggcgagcgg gccattctca ctcatggatt acttcctgcg
     2041 catggagggc ggcgcgttga gatccttggc gcagccgtcg agataatctt caagctcgcg
     2101 tttgttgcgc atgcggaatt tctgaaaatc cggtttgcgc ccctcgagaa aggcgtccat
     2161 gccctcgagg ctctcctccg agccccaaac atgggcgagc aattccatgc cgtgctgcca
     2221 ggaggcataa agcagatcgg actcgaagtt gaggctcttc ttggtcatgc gcagcgtctg
     2281 tgggctgtgg cccttgatgg tctcgcacca ggccagcgtt tccttcaaca ggtccttgga
     2341 gggtacgcac t
//
LOCUS       DATNAE010000293         2593 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2691027, whole genome shotgun sequence.
ACCESSION   DATNAE010000293 DATNAE010000000
VERSION     DATNAE010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2691027"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VN518_06570"
     CDS             <1..193
                     /locus_tag="VN518_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015670061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cobaltochelatase subunit CobS"
                     /protein_id="HXE04272.1"
                     /translation="TVMSPRTVIAWAENARIFGDVGFAFRLTFLNKCDELERPMVAEF
                     YQRSFGEELPESAANVALS"
     gene            200..2089
                     /gene="cobT"
                     /locus_tag="VN518_06575"
     CDS             200..2089
                     /gene="cobT"
                     /locus_tag="VN518_06575"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobT"
                     /protein_id="HXE04273.1"
                     /translation="MSSGPKRKEAPTEPLKRAIGLCVRAVAGHADIDVGFAPGAPEYT
                     PKHVQLPEPSRVPSQREIAVIRGWADSFALTAGCHDVRVHNKLAPSAGPARLVFEAAE
                     RARVEALGANRMPGMARNLTARIEDQYSHGRFAQVKTRADAPLEDAIALMIRERLTGL
                     PPPESTKALVDVWRASIEQRGSKFLKRLESLAEDQESFGRLMRDLLRALELTEDSVQA
                     DRDENTGDEEDKEEGSDGETETGEDGEDGSDQQREDGQAEGDVNQAQEASESASADEL
                     ESMLDDLEQFETPEQWRPNASVLENPEAFGYKVFTREFDEEIGAEQLSSPEELERLRA
                     YLDRELRALSSAVSRLANRLQRRLLAQQSRAWDFDLEEGTLDASRLPRVIIDPMHALS
                     FKRERDMEFRDTVVTLLLDNSGSMRGRPIMVAACCADILARTLERCGVKVEILGFTTR
                     AWKGGQAREQWLAAGKPQGPGRLNDLRHIIYKTADAPWRRAKRSLALMMREGLLKENI
                     DGEALAWAHRRLMARPEQRRILMMISDGAPVDDSTLSVNSGSYLEQHLRQVIDEIEMR
                     SPVELIAIGIGHDVTRYYRRAVTITDPSELAGAMTDQLVELFEEKVKVPQHLPGARLR
                     RRHLH"
     gene            2086..>2593
                     /locus_tag="VN518_06580"
     CDS             2086..>2593
                     /locus_tag="VN518_06580"
                     /inference="COORDINATES: protein motif:HMM:NF024841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HXE04274.1"
                     /translation="MSLRDFVGSHRGRRLRFPAGIAVVVAVGAGIAFGGTKPSTTDLQ
                     TGPIAVEARAIAGFDRLKRDATRFGKLTWRGGLILTSSSKHFGGWSGLAIDADGKGFF
                     AVSDAGTWMSGAISYDDKGLPQGLASVRLGAVQSKDGDPLSRSRDRDAEGLALLNGTT
                     QKGAVLVSF"
BASE COUNT          456 a          841 c          863 g          433 t
ORIGIN      
        1 gacggtgatg tccccccgga cggtgatcgc ctgggccgag aacgcgcgca tcttcggcga
       61 cgtcggcttc gccttccggc tgaccttcct caacaagtgc gacgagctcg agcggcctat
      121 ggtggcggag ttctatcagc gtagctttgg cgaggagctg cccgaaagtg cggccaacgt
      181 agcgctgagc tgacccgcca tgtcgagcgg acccaaacgc aaggaagcgc cgactgaacc
      241 gctgaagcgc gccatcggcc tttgtgtgcg tgccgtggcg ggccacgccg atattgacgt
      301 cggcttcgcg ccaggtgcgc ccgaatatac tccgaagcac gtgcagcttc ccgagccatc
      361 gcgtgtgcca tcgcaacgcg agatcgccgt cattcggggc tgggccgaca gcttcgcatt
      421 gacggccggg tgtcacgatg tgcgcgttca caacaagctg gccccgagcg ccggaccggc
      481 acgcctggtg ttcgaggcag cggagcgcgc gcgcgtcgaa gcgctcggcg ccaatcgcat
      541 gcctggcatg gcgcgcaatc tcacggcgcg catcgaggac cagtattcgc acgggcgctt
      601 tgcgcaggta aaaacccgcg ctgacgcgcc gctcgaggat gccatcgccc tgatgatccg
      661 cgaacgcctc accggcttac cgccgcccga aagcaccaag gcgctcgtcg acgtgtggcg
      721 cgcttcgatc gagcagcgcg gctctaagtt tctgaagcgg ctcgaaagcc ttgccgagga
      781 tcaggagagc ttcggccgcc tcatgcgcga cctgttgcgc gcgctcgagc tcaccgagga
      841 cagcgtgcag gctgaccgcg atgagaatac gggcgatgaa gaagacaaag aggaaggcag
      901 cgacggcgag acggaaaccg gcgaggacgg cgaggacggg agcgaccagc agcgcgagga
      961 tggtcaagcc gagggcgacg tcaaccaagc gcaggaagct tccgagtcgg cgagcgccga
     1021 tgagctcgag agcatgctcg acgatctcga gcagttcgag acgccggagc aatggcgccc
     1081 caacgcctcg gtcctcgaaa acccggaagc cttcggctac aaggtgttta cccgcgagtt
     1141 cgacgaggaa atcggcgccg agcagttgtc ctcgcccgaa gagcttgagc gcttgcgcgc
     1201 ctacctcgac cgtgagctgc gcgcattgtc gagtgccgtc tcccgcctcg ccaaccggtt
     1261 gcagcggcgc ctgctcgcgc agcagagccg cgcctgggac ttcgacctcg aggaaggcac
     1321 gctcgatgca tcgcgcctgc cgcgcgtcat catcgatccc atgcacgcac tgtcgttcaa
     1381 gcgcgaacgg gatatggagt ttcgcgacac ggtcgtgacg cttttgctcg acaactccgg
     1441 ctcgatgcgc ggccgcccga tcatggtcgc cgcctgctgc gccgatatct tggcgcgcac
     1501 gctggagcgc tgcggcgtta aggtagagat tctcggcttc acgacgcgcg cttggaaagg
     1561 tgggcaggcg cgcgaacagt ggctcgctgc cggcaagccg caggggcccg ggcgcctcaa
     1621 cgacttgcgt cacatcatct acaagacggc agacgcgcca tggcgccggg ccaagcgctc
     1681 gctggccctg atgatgcgcg agggtctgct gaaagagaac atcgacggtg aggcgctcgc
     1741 ctgggcgcac cgccggctta tggcgcggcc cgagcagcgc cgcattctga tgatgatctc
     1801 cgatggtgcg cccgtcgatg actcgacgct ctccgtcaac tcgggcagct acctggagca
     1861 gcacttgcgg caagtgatcg acgagatcga gatgcgttcg cccgtcgagc tgattgccat
     1921 cggcatcggc cacgacgtga cgcgctacta tcggcgcgcc gtcaccatca ccgatccgag
     1981 cgagctcgcc ggcgccatga ctgaccagct cgtcgagctg ttcgaagaga aggtgaaagt
     2041 accgcaacat cttcccggcg ctcgtctccg gcggaggcat ctccattgag cttgcgcgac
     2101 tttgtcggga gccatcgcgg ccggcgtctg cggtttcctg cgggcattgc ggttgttgtg
     2161 gcggtgggtg cgggtatcgc atttggcggc acgaaacctt cgaccaccga tctgcaaacg
     2221 gggcccatcg ccgttgaagc ccgcgctatc gccggcttcg atcggctgaa gcgcgacgcc
     2281 acacgcttcg gcaagcttac ctggcgtggg gggcttatcc ttacctcttc atcaaagcac
     2341 ttcggcggtt ggtcgggcct cgccattgac gcggacggca agggcttctt cgccgtgtcc
     2401 gacgccggca catggatgtc aggggctatc tcgtacgacg acaagggctt gccccagggc
     2461 ctggcttccg tgcggctcgg tgccgtccaa tccaaggacg gcgatccgtt gtcgcgctcg
     2521 cgcgaccgcg acgccgaagg gctggctctg ctgaacggca cgacgcaaaa gggcgcggtg
     2581 ctggtctcct tcg
//
LOCUS       DATNAE010000294         6804 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_1481750, whole genome shotgun sequence.
ACCESSION   DATNAE010000294 DATNAE010000000
VERSION     DATNAE010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6804
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1481750"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..923
                     /gene="corA"
                     /locus_tag="VN518_06585"
     CDS             <1..923
                     /gene="corA"
                     /locus_tag="VN518_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HXE04275.1"
                     /translation="PRRITEQTIWIDLLNPKREEERKVELALKLEVPTREEQREIEAS
                     SRLYQENGAYFMTATVLYQTETAEPITTPITFILAGNRLITLRYAEPHAFSIFAARCR
                     KPDPSLTTGTAVLIGLIETIVDRMADFIERIQGEVDTLSHSIFEMKGGTASRQRRFDV
                     ILKSIGREGELTSRARESAHSLGRLLTFLTQVANERKEDKLLKARVRTAARDVVSLAD
                     HVSYLSNKIVFLLDATLGMINIQQNDIIKIFSIAAVVFLPPTLVASAYGMNFEFMPEL
                     KWIFGYPFAILLMVVSAVLPYLYFKSKGWL"
     gene            1032..1340
                     /locus_tag="VN518_06590"
     CDS             1032..1340
                     /locus_tag="VN518_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE04276.1"
                     /translation="MTTRSLSIAAAAGLLLTAAMLPQHASALPGAGETQARPALVQQV
                     ASKYNRRYYRDNGTYVRAPFAEVDTRGGTWVAAPFARVYSGRRGTWVRAPFVDLWVPR
                     "
     gene            complement(1344..2648)
                     /gene="chrA"
                     /locus_tag="VN518_06595"
     CDS             complement(1344..2648)
                     /gene="chrA"
                     /locus_tag="VN518_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HXE04277.1"
                     /translation="MIERQSVAPSFKEALAVWAKIGVLSFGGPAGQIALMHRVLVDER
                     RWIGEDHFLRALNFCMLLPGPEAMQLATYVGWRLHGTLGGLVAGLLFVLPGALVVLAL
                     SIAYVLYGHLPLVEAAFVGIKAAVLVIVIEALLRVARRALRRPQDWIIAAAAFIAIFF
                     FAVPFPLVIAAAALIGYVLVANRPQANSPTETSPVRLTDTLCTAGLWLALWIVPLALL
                     ALLFGRDHVFTDIAIFFSKLAVVTFGGAYSVLAYMAQQAVETYGWLSAGEMLDGLGLA
                     ETTPGPLILVTEFVGFLAGYRHGGEPKLAFGLAGAAITLWATFVPCFLWIFVGAPYVE
                     RLSTNPRLASALSGVTAAVVGVILNLSLWFALHVFFGKVEVVRHGPLRLWLPDPSTLD
                     IEAVVLAAVAALLLLRLKLGIAATLAICASAALAWFMLSGVA"
     gene            2775..4637
                     /locus_tag="VN518_06600"
     CDS             2775..4637
                     /locus_tag="VN518_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013421157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HXE04278.1"
                     /translation="MVSTQLIQVAVFLAAAAIAAPLGRFFRMGAVLGYLAAGVVIGPY
                     ILGPLYQLNDVESILQFGEFGVVLLLFVIGLELRPIRLWAMRSAIFGLGTAQLAATSL
                     VLAAIGLALGLNFGQALFIGLGLSLSSTAFALQVLEEKGEINTRHGRLAFSVLLFQDL
                     AAIPLIALVPLFAAGGSEQPAMDLRAGVLAIFTIIGVIVAGRFLLSRLYRIIAATGVR
                     EAMTASALLTVVGVALVMEAAGLSAALGSFIAGALLADSEYRHQIEADIAPFEGLLLA
                     VFFIAIGMSIDLGIVVAEPLKLFAIVIGLVAIKAVILYALGRWWGLKSEPARRLGLAI
                     SQGGEFAFVLFAAGVGAHVIGQHLADLLVLAVTLSMAATPLLLLIDETIFRALEPEKP
                     EYEVPPEGDQHIIVAGFGRFGQIVARVLRARRIPFTALDSNVEQVDFVKRFGSRIYYG
                     DAGRLDILRAAGADKARAFVLAIDDVDASLRVAEMVRKHFPDLPVYARARDRIHVHKL
                     MDLGVEIIERETFLSALELTHRLLRGLGLSEPEAKRLLATFKQQDEKRLYDDYKYYTD
                     LEKVRANAKTAAVELEELFARDVDELKLPEEERALVGAERRDGRRPTGSPRKDG"
     gene            complement(4549..5394)
                     /locus_tag="VN518_06605"
     CDS             complement(4549..5394)
                     /locus_tag="VN518_06605"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXE04279.1"
                     /translation="MSLKQMTVNGVEIAYLDRGNGPPVIIAHCSSASHREWLPLIEAL
                     EPDWRVLAPDFIGYGQSGAWPEGKVFTGQADLAVLLDLANKAERPIHLVGHSYGAALA
                     LEVARGLGSKVQSLTLVEPVAFNLLRVERRPEWAEIEQLGVAVLTAVSNGNDRDAASA
                     FMRYWLGRLRWWLSPEKFKAAITATIHKVALEFMILIEAGARLSDYASVTAPTLLIVG
                     GKTRAPARAVVNMLSQVLPNATTTVLKGAGHMSPFTHPSEVNRLVAGHLAAQRQQEPA
                     PLPEA"
     gene            complement(5540..6436)
                     /gene="rpoH"
                     /locus_tag="VN518_06610"
     CDS             complement(5540..6436)
                     /gene="rpoH"
                     /locus_tag="VN518_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HXE04280.1"
                     /translation="MASQSLPSVGAHGGLTRYLEEIRQFPMLEPQQEYMLAKSWREHG
                     DRDAAHKLVTSHLRLVARIAMGYRGYGLPIGEVVSEGNVGLMQAVKRFDPDKGFRLAT
                     YAMWWIRAAIQEYILRSWSLVKMGTTAAQKKLFFNLRKIKGQLKALDEGDLRPDQVKR
                     IATQLGVTEEDVVSMNRRLAGDSSLNAPVRNDSESGEWQDWLVDDTMDQETALAESEE
                     AQRRRGMLNNALKGLNERERRVFEARRLAEDPLTLEELSAEFGVSRERIRQIEVRAFE
                     KVQKAVQKAAAAAEVPEAAAAS"
     gene            complement(6544..>6804)
                     /locus_tag="VN518_06615"
     CDS             complement(6544..>6804)
                     /locus_tag="VN518_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HXE04281.1"
                     /translation="GRTHQVRVHLASIGTPLIGDPIYGTGFKSKFRKLPQAAQDKIVS
                     FKRQALHAAALSFVHPLTGTLLEFNSPLPDDFAMVVDALKEL"
BASE COUNT         1258 a         2134 c         2173 g         1239 t
ORIGIN      
        1 atccgcgacg aatcactgag cagacgatct ggatcgatct cctcaatccc aagcgggaag
       61 aagagaggaa ggtcgagctt gcgctcaagt tggaggtacc gacccgcgaa gagcagcgtg
      121 agatcgaggc gtcgagccgc ctctatcagg aaaacggcgc gtacttcatg accgccacgg
      181 tgctgtatca gaccgagacg gcggagccca tcaccacgcc catcaccttc atcctcgccg
      241 gcaacaggct catcaccctc cgctatgcgg agccgcacgc cttctcgatc ttcgccgccc
      301 gctgccgcaa gccggaccca agcctcacca ccggcacggc ggtgctgatc gggctgatcg
      361 agaccatcgt cgatcgcatg gcggatttca tcgagcgcat ccagggagag gtcgacacgc
      421 tgtcgcattc gatcttcgag atgaagggag gcaccgcctc gcggcagcgc cgattcgacg
      481 tgattctgaa atcgatcgga cgcgaaggcg agctcacctc gcgtgcgcga gagagtgcgc
      541 attcgcttgg gcgtctgctc acctttctca cccaggttgc caatgagcgg aaagaggaca
      601 aattgctgaa agcgcgagtc cgcaccgcgg cccgcgacgt ggtgtcgctc gccgatcatg
      661 tgagctattt gtcgaacaag atcgtgttcc tgctcgacgc gacgctcggc atgatcaaca
      721 tccagcagaa cgacatcatc aagatcttct ccatcgccgc ggtggtgttc ctgccgccga
      781 ctctggttgc gtcggcctac ggcatgaact tcgagttcat gcccgagctg aaatggattt
      841 tcggttatcc cttcgccata ttgctcatgg tggtttcggc ggtgcttccc tatctctatt
      901 tcaagagcaa gggatggctg tagttaatgt gtcgctgcca cggcgaaccg cctcgcctct
      961 gtcgcgttgc cgtagtagga agatgggaag acgggtgggg gtcgaacgaa tatgcgtagg
     1021 agaaaatgtg catgaccact cgatccttga gtatcgccgc ggccgctggc ctgctcctca
     1081 ctgccgccat gctgccgcag cacgcgtcgg cgctgcccgg tgccggcgag acgcaagcgc
     1141 gacccgctct cgttcaacag gtcgcgagca aatacaatcg ccgttactac cgcgacaacg
     1201 gcacctatgt gcgggcgccg ttcgccgagg ttgacacacg cggcggcact tgggttgcgg
     1261 cccccttcgc ccgtgtttac tccggtcggc gcggcacttg ggtgcgggcg cccttcgttg
     1321 atctctgggt tccgcgctaa aggctaagcc actccggaca gcatgaacca ggcaagcgcc
     1381 gcgcttgcgc aaattgcgag cgtggcggcg atgccgagct tcagtctgag cagcagaagc
     1441 gccgcgacgg ccgcaagcac gacggcttca atgtcgagcg tcgaggggtc gggcaaccaa
     1501 agccgcaatg gaccatgccg aaccacctcg accttgccga agaaaacgtg cagtgcgaac
     1561 cacaggctca agttgagaat gacgccgacc acggcggcgg taacgccgct cagcgcgctt
     1621 gcgaggcgcg ggttggtgct gagacgctcc acgtagggcg cgcccacgaa gatccacagg
     1681 aagcagggca cgaaggtcgc ccatagcgtg atggcggcgc cagccaagcc gaaggcgagc
     1741 ttaggttcgc cgccgtggcg gtagcctgcg aggaagccga cgaactcggt gacgaggatc
     1801 aggggccccg gcgtcgtttc ggcgagaccc aagccgtcga gcatctcgcc ggcgctcagc
     1861 caaccataag tctcgaccgc ctgctgcgcc atataggcga ggaccgaata ggcgccgccg
     1921 aaggtgacca cggccagctt ggaaaagaag atggcgatgt cggtgaagac gtgatcgcgc
     1981 ccaaacagaa gcgcgaggag cgctaaggga acgatccaga gcgcgagcca gaggccggca
     2041 gtgcacagag tatcggtaag ccgcacaggg ctcgtctcag tgggcgaatt cgcttgcggg
     2101 cggttcgcca ccaggacgta gccaatgaga gccgccgcgg cgatgacgag agggaacggc
     2161 acggcgaaga agaagatggc gatgaaagcc gccgcggcaa tgatccaatc ctgaggacgc
     2221 cgcaaagcgc gccgtgccac cctcagcaat gcctcgatga cgatgaccag cactgccgcc
     2281 ttgatgccga cgaacgccgc ctcgacgagc ggcaaatggc cgtagagcac ataagcgatg
     2341 gaaagtgcga gcacgacgag ggcgccgggg agcacgaaga gcaaccctgc aacgagcccg
     2401 cccaaggtgc cgtgcagccg ccagccgaca taagtcgcaa gctgcatggc ttcggggccg
     2461 ggcagcagca tgcagaaatt gagcgcgcga aggaaatggt cctcgccgat ccagcgacgc
     2521 tcatccacca gcacgcggtg catcagcgcg atctgtccgg cggggccccc gaacgacaag
     2581 acgccgatct tcgcccagac ggcgagtgct tctttgaagg acggagcaac gctctggcgt
     2641 tcgatcatgc ggctttggta ccggattttg tgccgtgccg gaatgatagc aatctcggct
     2701 tgcgctcaaa tctaggtctt gcccagagag cgtgatacgg aagacggaaa gggcttgggg
     2761 cacaaggacg agcgatggtt tcgacacaat tgatccaagt ggcggtgttt ctcgccgccg
     2821 ccgccatcgc cgcgccgctt ggccgcttct ttcgcatggg agccgtgctc ggctatctcg
     2881 ccgccggcgt ggtgatcggt ccctacattc tcgggccgct ctatcagctg aacgacgtcg
     2941 agagcatcct gcaattcggc gagttcgggg tggttctgct gctgttcgtg atcgggctcg
     3001 agcttcgccc gatcaggctt tgggccatgc gctcggcgat cttcgggctg ggcacagccc
     3061 agctcgcggc cacgtcactg gtgctcgccg ccatcggtct tgcgcttggc cttaactttg
     3121 gccaggcgct cttcatcggc cttgggctgt cgctctcgtc gacggctttc gcgctgcagg
     3181 tgttggagga gaaaggcgag atcaatacca ggcacggcag gctcgccttt tcggtgctcc
     3241 tgttccagga ccttgccgcc attccgctga tcgcgctcgt gccgctgttt gcggcaggcg
     3301 gcagcgagca gccggccatg gatctccgag ccggcgtgct cgccatcttc accatcatcg
     3361 gcgtgatcgt cgccggacgc ttcctgctca gcaggcttta tcgcatcatc gcagccaccg
     3421 gcgtgcgcga ggcaatgacg gcgagtgcgc tgctcaccgt ggtcggcgtg gcgctggtca
     3481 tggaagctgc gggcctcagt gcagcgcttg gctcgttcat cgccggcgct ctgctcgccg
     3541 actcggagta ccgccatcag atcgaagccg acatcgcgcc cttcgaggga ctgctgctcg
     3601 cggtgttctt catcgccatc ggcatgtcga tcgacctggg cattgtggtg gcggagccgc
     3661 tcaagctctt cgccatcgtc atcggccttg tcgctatcaa ggccgtgatt ctctacgcgc
     3721 tcggccgctg gtgggggttg aaaagcgagc cggcgcggcg gctcgggctc gcgatcagtc
     3781 agggcggcga gtttgccttc gtgctgttcg cggcaggggt cggcgcgcac gtcatcggcc
     3841 agcacctggc cgatctactc gtgttggcgg tgaccttgtc catggcggca acgccgctcc
     3901 tgctgctcat cgacgagacg atcttccgcg cgctcgagcc cgagaagcca gaatacgagg
     3961 tgccgccgga aggcgatcag cacatcatcg tcgcaggctt cggccgcttc gggcagatcg
     4021 tggcccgcgt gcttcgtgcc cgccgcattc ccttcacagc gctcgactcc aatgtcgagc
     4081 aagtggactt cgtgaagcgc ttcggctcgc gaatctatta tggcgatgcc gggaggctcg
     4141 acattctgcg cgcggccggc gccgacaagg cgcgcgcctt cgtgctcgcc atcgacgatg
     4201 tcgacgcctc gctcagggtg gccgagatgg tgcgcaagca ctttcccgac ctgccggtct
     4261 atgcgcgggc gcgcgaccgg atccatgtgc acaagctcat ggatctcggc gtcgaaatca
     4321 tcgagcgcga gacgttcctc tcggcgctcg aactgacgca caggctgctg cgagggctcg
     4381 ggcttagcga gcccgaggcc aagcggctgc tcgcgacgtt caagcagcag gacgagaagc
     4441 ggctgtacga cgactacaaa tattacaccg atctcgagaa ggtgcgcgcc aacgcgaaga
     4501 ccgcggcggt ggagctcgag gagttgttcg cccgcgacgt ggacgaactc aagcttccgg
     4561 aagaggagcg ggctcttgtt ggcgctgagc ggcgagatgg ccggcgacca accggttcac
     4621 ctcggaagga tgggtgaacg gactcatatg gccggcgcct ttgagcaccg tcgtggtggc
     4681 attcggcagg acctggctca gcatgttgac cacagcgcgg gccggagcac gcgtcttgcc
     4741 gccaacgatc agcagagttg gcgccgtgac gctcgcatag tcgctcaagc gcgcgccagc
     4801 ctcgatcagg atcatgaatt cgagcgcgac cttgtgaatg gtcgcggtga tggcggcctt
     4861 gaatttctcc ggcgacagcc accagcgcaa gcggccgagc caatagcgca tgaaggctga
     4921 agcggcgtcg cggtcgttgc cgttcgacac ggcggtcagc acggcgacgc cgagctgctc
     4981 gatctcagcc cattcagggc gacgctcaac gcggagcagg ttgaaggcga ccggctcgac
     5041 cagggtgagg ctctgcacct tggacccgag cccgcgcgcg acctcaagcg ccaaggccgc
     5101 gccataggag tggccgacca agtggatggg cctctcggct ttgttggcta gatcgagcag
     5161 cacggcgaga tcggcctgac cggtgaacac cttgccctca ggccaagcgc ccgactgccc
     5221 atagccgata aaatcggggg cgagcacgcg ccaatcgggc tcaagcgcct cgatcagcgg
     5281 cagccattct cggtgcgagg cagagctgca atgggcgatg atgacggggg gcccgttgcc
     5341 ccggtcgaga taggcgatct cgacgccgtt taccgtcatc tgcttcaggg acatcaggcc
     5401 ttactgccat tcctcgctgt cattgcccgg cttgaccggg caatccagta acccctgcat
     5461 tcatcgatct gtgagatcag tgattactgg attcccgctt gcgcgggaat gacacggaga
     5521 acctgaaagg taggggacgt cagcttgcgg ccgcggcttc cggaacttcg gctgcggcag
     5581 cagccttctg caccgccttc tgcaccttct cgaaggcgcg cacttcgatc tggcggatgc
     5641 gctcccggga gacgccgaac tcggccgaca gctcttccag cgtcagaggg tcctcggcaa
     5701 gccgccgcgc ttcgaacact ctccgctcgc gctcgtttag gcccttcagc gcgttgttga
     5761 gcatgccacg ccgccgctga gcctcttccg actcggcgag cgccgtctcc tggtccatgg
     5821 tgtcgtcgac cagccagtcc tgccactcgc cgctttcgct gtcgttgcgc accggcgcgt
     5881 tcaatgacga gtctccggcg agcctccggt tcatggagac gacgtcttcc tcggtcaccc
     5941 cgagctgggt ggcgatgcgc ttcacctgat cggggcgcaa atcgccttcg tcgagggcct
     6001 tgagctgacc cttgatcttg cgcaggttga agaagagctt cttctgcgca gcggtggtgc
     6061 ccatcttcac gaggctccac gaacgcagga tgtattcctg gatcgcggcc ctgatccacc
     6121 acatggcgta ggtggcaagc ctgaagccct tgtcgggatc gaagcgcttc accgcctgca
     6181 tcagcccgac attgccttcc gagacgacct cgccgatggg cagcccgtag ccgcgatagc
     6241 ccatggcgat gcgggcaacg agacggagat gggaggtgac gagtttatgg gctgcgtcgc
     6301 ggtcgccatg ctcgcgccag ctcttagcga gcatgtactc ctgttgaggc tccagcatgg
     6361 gaaattgccg gatctcttcc aggtagcgcg tcaggccgcc atgcgcgccg acactcggta
     6421 gactctgcga ggccatgaga gcaattctcc cgcggcctga tgccggccgc atcttttcat
     6481 cttaatgccc cccggcgccc ctctatagaa cgtttgcctc aaggattaaa gaccgaggaa
     6541 gcttcacagt tccttgaggg cgtcgaccac catggcaaaa tcatcgggca ggggactatt
     6601 aaattccagt aaggtcccgg tcagcggatg cacgaagctt agtgcggcag cgtgcagagc
     6661 ctggcgcttg aaacttacaa ttttatcttg tgcggcttgc ggaagcttgc ggaacttgga
     6721 tttaaagccg gttccgtaga tcggatctcc gatcaaaggc gtgccgatcg aggcgagatg
     6781 cacccgcacc tgatgggtgc ggcc
//
LOCUS       DATNAE010000295         2354 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_289373, whole genome shotgun sequence.
ACCESSION   DATNAE010000295 DATNAE010000000
VERSION     DATNAE010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289373"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..1359
                     /gene="argH"
                     /locus_tag="VN518_06620"
     CDS             13..1359
                     /gene="argH"
                     /locus_tag="VN518_06620"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011160275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HXE04282.1"
                     /translation="MEEINASIDFDKRLWRHDILASQAHIAMLGATGIVTAKEAQEIA
                     RGLDQIMAEIEVGTFKFSRALEDIHLNVESRLTELIGPVAGKLHTARSRNDQVATDFR
                     LYVREEIGTIDGALAALQLALATKALEHAGTVMPGFTHLQTAQPITFGHHLLAYVEML
                     GRDRGRFADAAKRLNESPLGAAALAGTPFPIDREATASALGFARPAANSLDAVSDRDF
                     ALEALAAASICAMHLSRLAEEVVNWTSPQWGFARLSDQFTTGSSIMPQKRNPDAAELV
                     RAKTGRIAGAFQALLMVMKGLPLAYAKDMQEDKEPVFDALDALKLGIAAMTGMVVDLE
                     PDVKAMKRAASAGHSTATDLADWLVLNLGLPFREAHHVTGKLVALAESRGCDLKKLPL
                     SELKAVDKRITEQVYPALDVAGSVKSRTSYGGTAPSNVKREARRWIKRLEKEGQTR"
     gene            1607..>2354
                     /locus_tag="VN518_06625"
     CDS             1607..>2354
                     /locus_tag="VN518_06625"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HXE04283.1"
                     /translation="MHHFAYRRGVSHQNVLHAEEVDLADLAATVGTPFYCYSSATLER
                     HYRVFADAFAGAPDTLVCYSVKANSNLGVLATLGRLGAGMDIVSEGELRRALAVKVAP
                     ERIVFSGVGKTQAEMAVALDAGILAFNVESEPELRALSEVAASRGPTARIAFRVNPDV
                     DAKTHHKIATGKAENKFGVPFAQAPALYALASKLPGIEASGVHMHIGSQITDLEPFRN
                     AFALLHELVADLRGQGFNISFVNLGGGLGIP"
BASE COUNT          438 a          758 c          749 g          409 t
ORIGIN      
        1 cccgcgcaga tcatggagga gataaacgcc tccatcgact tcgacaagcg gctgtggagg
       61 cacgatattc tcgcctcgca ggcgcatatc gccatgctcg gcgcgaccgg catcgtcacg
      121 gctaaagagg cgcaggagat cgcccgcggc ctcgaccaga tcatggccga gatcgaagtc
      181 ggcacattca aattctcgcg cgcgctcgag gacattcatt tgaatgtcga gagccggctc
      241 accgagctga tcgggccggt cgccggcaag ctgcataccg cgcgctcgcg caacgaccag
      301 gtggcgacgg acttcaggct ctatgtcagg gaggagatcg gcacgatcga cggcgcgctc
      361 gccgcgctcc agctggcgct ggcgaccaag gcgctcgagc atgcgggaac ggtgatgccg
      421 ggcttcaccc atctccagac cgcgcagccg atcaccttcg gtcatcatct gctggcttat
      481 gtcgagatgc tggggagaga cagaggccgc ttcgccgacg cggccaagcg gctgaacgaa
      541 tcgccgctcg gcgccgcagc ccttgccggc acacccttcc ccatcgaccg ggaggcaacc
      601 gcgagcgcgc tcggctttgc caggccggcg gcgaactcgc tcgacgcggt gtcggatcgc
      661 gacttcgcgc tcgaagcgct ggcggccgcg tcgatctgcg ccatgcatct gtcgcggctc
      721 gccgaggagg tcgtcaactg gacctcgccg caatggggct tcgcgcgact ctcggaccaa
      781 ttcaccaccg gctcatcgat catgccgcag aagcgcaacc ccgacgcggc cgagctggtg
      841 cgcgccaaga ccggacgcat tgccggcgcc tttcaagcgc tgttgatggt gatgaaaggc
      901 ctgccgctcg cctatgccaa ggacatgcaa gaggacaagg agccggtgtt cgacgcgctc
      961 gatgcgctga agctcggcat cgccgccatg accgggatgg tcgtggacct cgagccggac
     1021 gtgaaggcga tgaagcgggc ggcttcggcc gggcattcga ccgcgaccga cctggccgac
     1081 tggctggtgc tcaatctcgg acttcctttc cgggaggcgc accatgtcac cggcaagctt
     1141 gtggccttgg ccgaatcaag gggctgcgac ctcaaaaaac tgccgctttc ggagcttaag
     1201 gcggtggaca agcgtattac cgaacaggtt tatccggcgc tggatgtcgc aggatcggtg
     1261 aaaagccgca ccagttatgg cggaaccgcc ccttccaacg tcaaacggga ggcgagacgg
     1321 tggataaagc gtttggagaa ggaaggccaa acgcgataaa aggataggtc aagggccaag
     1381 taataggccg agggacaggg gcacgaccat gggaagcgct ttgcgcatcc ttttatcgct
     1441 tgtgctgatg gcggccatcg ggcttacagc ttgtgggcgc cgtggctcat tggaccgacc
     1501 gccgcccact taccctcaga ccaaggccga tccgtccacg aaaatgtcgc aggctcctga
     1561 ccggaagctt tggatcgatc gactgctgca atagacctcc cgagaaatgc accatttcgc
     1621 ctaccggcgc ggcgtctccc accagaatgt gctccacgcc gaggaggtcg atcttgccga
     1681 cctcgctgcg accgttggaa ctcccttcta ttgctactcg tcggcgacgc tggagcgtca
     1741 ctatcgggtg ttcgccgatg ccttcgccgg cgcgccggat acgctcgtct gctattcggt
     1801 caaggcgaac tcaaatctcg gcgtgctcgc cacgctcggc cggctcggcg ccggcatgga
     1861 catcgtgtcc gagggcgagc ttcgccgcgc gctggcggtc aaggtcgcgc ccgagcgaat
     1921 cgtgttttcg ggtgtcggca agacgcaggc cgagatggct gttgcgctcg acgcgggcat
     1981 cctcgccttc aatgtcgaat cggagcccga gctcagagcg ttgagcgagg tggccgcgag
     2041 ccggggcccg accgcgcgca tcgctttccg cgtcaatccg gatgtcgacg ccaaaactca
     2101 ccacaagatc gcgacgggaa aggccgagaa caaattcggc gttccgttcg cgcaagcccc
     2161 ggccctttac gcgctggcga gcaagctgcc cggcatcgaa gcctccggcg tgcatatgca
     2221 tatcggcagc cagatcaccg accttgagcc gttccgcaat gcgttcgcgc tgttgcacga
     2281 attggtcgct gatctgcgag gccagggctt caatatctcc ttcgtcaatc tcggcggcgg
     2341 cctcggcatt ccct
//
LOCUS       DATNAE010000296         2937 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2693736, whole genome shotgun sequence.
ACCESSION   DATNAE010000296 DATNAE010000000
VERSION     DATNAE010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2693736"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..1165
                     /locus_tag="VN518_06630"
     CDS             209..1165
                     /locus_tag="VN518_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011475047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HXE04284.1"
                     /translation="MIEKLEFIIALARERHFGHAAEACGVSQPTLSAGIKQLEDSFGV
                     LLVQRGSRFQGFTPEGERVLDWARRIVGDTRAMHQEIDALKRGLAGHIRIAAIPTALA
                     MVELLTTPYRAKHPDVSFTIWSRNSIEVLGLLENLEIDAGLTYLDNEPLGRVQTVALY
                     SEEYRLLTSSDAPLGNRDSVTWAEVGKVPLCLLTPDMQNRRIIDGLLREAGAASSPTL
                     ESDSILVLFAHVRTGRWASVMPAKLASVLGLTDTVRAIPIVKPEVNHSIGLVVPHRNP
                     LTPLVNALVNEAKQLAPILGSDSPPKPRRKRDMKIRSPDRTL"
     gene            1335..1772
                     /locus_tag="VN518_06635"
     CDS             1335..1772
                     /locus_tag="VN518_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HXE04285.1"
                     /translation="MIIDANNHREGAALPVLHALQHVFGCVPEAAVPLVAEALNLSRA
                     EIYGTVTFYHDFRNEPPGRRVLKLCRAESCQAAGGDALAARAEEKLGVKFGETTPDAR
                     VTLEAVYCLGLCHSSPAALLDHDHVVGMLDEAKLDALLKEAQA"
     gene            1769..>2937
                     /locus_tag="VN518_06640"
     CDS             1769..>2937
                     /locus_tag="VN518_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014766164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HXE04286.1"
                     /translation="MRARVFTSRDSGALCVGAEEVARALFKAAEKRGLDVEIVRTGSR
                     GMYWLEPTIEVARPEGRIGYGPVKPSDIDSLLDAGMLDGGKHPLSLGLVEDIPFFKKQ
                     TRLTFARVGIIDPRSLEDYKAHGGYKGLEKAIAVGPEAIVEEVVNSGLRGRGGAGFPT
                     GIKWRTVAQTQPQQKYIVCNADEGDSGTFADRMIMEGDPLVLIEGMTIAGIAVGATYG
                     YVYIRSEYPHAVDAMEIAIKRAREGGMLGANVAGSKYEFDMEVRVGAGAYVCGEETSL
                     LESLEGKRGEVRAKPPLPAHVGLFGKPTVINNVLSLASVPIIMSDGAAYYRDFGMGRS
                     RGTMPIQLAGNIKHGGLFETAFGMSLGELVDEIGGGTRTGRPVRAVQVGGPLGAY"
BASE COUNT          521 a          964 c          923 g          529 t
ORIGIN      
        1 ttgacgtccg tccgaagctc gaaagcgcgc aagatcgcat catgaccgac caacagcggt
       61 cggacaggca aaaatagtga atgctcaagg cgtccactgc gggagccgtc gctgaattgt
      121 catactcttc ggcgccatct caacccttgc actggcgctt acggcttctt cttgcgaaga
      181 taaggaccgc tccggcaggc cccacggcat gatcgagaaa cttgaattca tcatcgcgct
      241 cgcccgcgag cggcatttcg gccatgccgc cgaggcctgc ggcgtgagcc agccgacgct
      301 gtcagcaggg atcaagcagc tcgaggattc cttcggggtt ctgctggtgc agcgcggctc
      361 gcgcttccaa ggcttcaccc ctgaaggcga gcgggtgctc gactgggcgc ggcgtatcgt
      421 gggcgatacc cgcgccatgc atcaggagat cgacgcgctg aaacggggcc ttgccggcca
      481 tatccgcatc gccgccatcc ccaccgcgct tgccatggtc gagctgctca ccacgcccta
      541 ccgcgccaag catcccgacg taagcttcac catctggtcg cgcaattcca tcgaggtgct
      601 tggactgctt gagaatttgg agatcgacgc cggcctcacc tatctcgaca acgagcctct
      661 cggccgggtg cagacggtgg ccctctatag cgaggagtac cggctcctga cctcctcaga
      721 tgcgccgctc ggcaatcgcg acagcgtcac ctgggctgag gtgggcaagg tgccgctttg
      781 cctgcttact cccgatatgc agaaccggcg aatcatcgac ggcctgctgc gcgaggctgg
      841 cgccgcgtcg tccccaacgc tcgaatccga ctcgatcctc gtgctgttcg cccatgtgcg
      901 caccggacgc tgggccagcg tcatgcccgc caagctcgcc tcggtgctcg gcctgaccga
      961 tacggtacgg gccattccca tcgtcaagcc tgaggtgaac cattcgatcg gtctcgtcgt
     1021 ccctcaccgc aatcccttga caccgctcgt caatgcgctg gtcaacgagg ccaagcagct
     1081 tgccccgatt ttgggcagcg acagtccgcc aaagccgcgg cgcaagcgtg atatgaaaat
     1141 ccgatcgccc gatagaactt tgtaatcaaa gggaaatctg gctctgcgcg cttgcgatgc
     1201 agggggccgc ataatatctt tgcatgagga tcaaagcgct ccgctgggga acacggtggc
     1261 tgtcgatttt tgtcatccaa gggatgctgc ctgcgcatgc cgagatatga gccgtggagc
     1321 gccgagcgcg ccaagtgatc atcgacgcga acaatcatcg cgagggcgcg gcgctgcccg
     1381 ttttgcatgc gctgcagcac gtctttggct gtgtgccgga agccgccgtg ccgctggtgg
     1441 cggaggcgct caacctgtcc cgtgccgaaa tctacggcac ggtgaccttc tatcacgact
     1501 tccgcaacga gcctcccggt cggcgcgtgc tcaagctttg ccgcgccgaa tcctgccagg
     1561 ccgcaggcgg cgatgcattg gcagcgcgcg ccgaggagaa gctcggcgtg aagttcggcg
     1621 agaccacgcc ggacgcccgc gtgacactcg aggccgtcta ttgcctcggg ctttgccata
     1681 gctcccccgc agcgctgctc gatcacgatc atgtggtggg catgctcgat gaggcgaagc
     1741 tcgacgccct cttgaaagag gcgcaggcat gagggcgcgt gtgttcacct cgcgggattc
     1801 aggcgcgctc tgcgtcggcg ccgaagaagt ggcgcgcgct ttgttcaaag ccgcagagaa
     1861 gcgtggcctc gacgtcgaga tcgtgcgcac cggctcgcgc ggcatgtact ggctcgagcc
     1921 gacgattgaa gttgcgcggc cagagggccg catcggctat gggccggtga agccctccga
     1981 tatcgacagc ctgctcgatg ccggcatgct ggacggcggc aagcatccgc tctccctcgg
     2041 cctcgtcgag gacatcccgt tcttcaagaa gcagacccgc ctcaccttcg cccgcgtcgg
     2101 catcatcgat ccgcgctcgc tcgaggacta caaagcccat ggcggctaca aggggctgga
     2161 aaaggcgatt gccgtcggcc ccgaggcgat cgtcgaggag gtggtgaatt caggcttgcg
     2221 cggccgcggc ggcgccggct tccccaccgg catcaaatgg cgcaccgtcg cgcagaccca
     2281 gcctcagcag aaatacatcg tgtgcaacgc cgatgagggc gattcaggca ccttcgccga
     2341 ccgcatgatc atggaaggcg atcccctcgt gctgatcgag ggcatgacca tcgccggcat
     2401 cgccgtcggc gcgacctacg gctacgtcta tatccgctcc gaatatcccc atgccgtcga
     2461 cgccatggag atcgcgatca agcgcgcccg cgagggcggc atgctcgggg ccaatgtcgc
     2521 cggctccaaa tatgaattcg acatggaggt tcgggtcggc gccggggcct atgtgtgcgg
     2581 cgaggagacc tcgctgctcg agagccttga aggcaagcgc ggcgaggtcc gcgccaagcc
     2641 tccgctgccg gctcatgtcg gcctgttcgg caagccgacc gtcatcaaca atgtgctgtc
     2701 gcttgctagc gtgccgatca tcatgagcga tggcgccgcc tattaccgcg atttcggcat
     2761 gggccgctca cgcggcacca tgccgatcca gctcgcaggc aacatcaagc acggtgggtt
     2821 gttcgagact gccttcggca tgagccttgg cgagctcgtc gacgagatcg gcggcggcac
     2881 gcggaccggg cgcccggtgc gcgcggttca ggtcggcggc ccgcttggcg cctattt
//
LOCUS       DATNAE010000297         2236 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3895043, whole genome shotgun sequence.
ACCESSION   DATNAE010000297 DATNAE010000000
VERSION     DATNAE010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3895043"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="VN518_06645"
     CDS             complement(<1..262)
                     /locus_tag="VN518_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HXE04287.1"
                     /translation="MKTLLPASRGYFAIGVEGISKAVNLGNLLRSAHAFGASFVFTLG
                     ADETAIEIRHDTSKAWTHLPVYHWRSLADLRLPKGCSLVGVEI"
     gene            336..1316
                     /locus_tag="VN518_06650"
     CDS             336..1316
                     /locus_tag="VN518_06650"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HXE04288.1"
                     /translation="MRVAQFSEFGGPQALRLEQVAEPSPGPGDVLIKVTAVGLNFFDT
                     LMLRNQYQVTPPLPFSPGAEIAGSIEALGAEVADLKLGQRVVAFIGGNGCREKVVTKA
                     RNVVPIPDGVSDEVAAGIPITYGTALHGLKDRGALQPGESVAILGAAGGAGVAAIEIA
                     KLMGGRVIAVASSADKLAFAREHGAEEGINYETEDLKARLKQLTAPKGVDVLYDPVGG
                     AYAEPSLRAMAWEGRYLVLGFASGTIPKIPLNLVMLKGCAIMGVFWTAFVERYPEKHR
                     ANMIELLDWCRRGLITPHIHASFALIETPKALSLIEGRKVTGKVIVNPQR"
     gene            complement(1313..1711)
                     /locus_tag="VN518_06655"
     CDS             complement(1313..1711)
                     /locus_tag="VN518_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008933658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HXE04289.1"
                     /translation="MAFNEASELKAAAFGRQLSLSAFAERERKVRRDFWAKLKRFAGM
                     IPFVDDLVAAYYCALDPATPMRVRGMLLAAIAYFILPVDIIPDMIAGLGFADDAALLT
                     AVIGLVASHITPTHRAAAARALDKELPKAR"
     gene            1912..>2236
                     /locus_tag="VN518_06660"
     CDS             1912..>2236
                     /locus_tag="VN518_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE04290.1"
                     /translation="MPILAGALLAALAALYCAIRVPADAMTGQQAPPWTVTPLDATVA
                     SKLRQLDALRRDMEQAGCEYAADDASIEGCQQLDATARRLEAEIEELKARAGWTLEKQ
                     EAVEAG"
BASE COUNT          421 a          704 c          751 g          360 t
ORIGIN      
        1 agatctcgac tccgaccagg ctgcagcctt tcggcaatct gagatcggcg aggctccgcc
       61 aatgatagac gggaagatgc gtccaggcct ttgacgtgtc gtgacggatc tcgatcgcgg
      121 tctcgtcggc gcctagggtg aacacgaagc tggcgccgaa ggcatgcgcc gagcgcagca
      181 gattgccgag attgacggcc ttggagatgc cctcgacgcc gatggcgaaa taaccgcggg
      241 aggcgggaag tagggttttc acaagtcttg tctgccacaa gctgggtgag agaaaaaggg
      301 tagccagtcg ttacccaaaa aggaggcaag cttcgatgcg ggttgcgcaa ttcagcgaat
      361 tcggcggacc tcaggcgctt cgcctcgagc aggtggccga gccgagcccc ggccccggcg
      421 atgtcctgat caaggtgacg gcggtcggcc tcaacttctt cgacacgctc atgctgcgca
      481 atcaatatca agtgaccccc ccgctcccgt tctcgccggg agcggaaatc gccggcagca
      541 tcgaagcgct tggcgcagag gtcgccgacc tcaagctggg gcaaagagtc gtcgccttca
      601 tcggcggcaa tggttgccgt gagaaggtcg tcaccaaggc gaggaacgtg gtccccatac
      661 ccgacggcgt gagcgatgaa gtggcggccg gaattcccat cacctacggc acagcgctgc
      721 acggattgaa ggaccggggc gccttgcagc cgggcgagag cgtggcgatc ctcggcgccg
      781 cgggaggagc gggtgtcgcc gccatcgaga tcgccaagct catgggtgga cgggtgatcg
      841 ccgtcgcctc gtcggccgac aagctcgcct ttgcccgcga gcatggcgcc gaggaaggca
      901 tcaattacga gacggaagat ctcaaggccc gcctcaaaca gctcaccgcg ccgaaaggcg
      961 tcgacgtgct ctacgatccg gtgggcggcg cttatgccga gccgagccta cgggcgatgg
     1021 cctgggaagg ccgctatctc gtgctcggct tcgcctcggg cacaattccg aaaattccgc
     1081 tcaacctggt catgctcaag ggctgcgcca tcatgggcgt gttctggacg gcgttcgtcg
     1141 agcgctatcc ggaaaagcac cgcgccaaca tgatcgagct gctcgattgg tgcagacgcg
     1201 ggctgatcac gccgcacatc cacgcgagct ttgccttgat cgagaccccc aaggccctgt
     1261 cgctgatcga gggccgcaag gttaccggca aggtgatcgt caacccgcag cgctagcggg
     1321 ctttgggcaa ttccttatcg agcgcgcgtg ccgccgccgc cctgtgggtc ggcgtgatgt
     1381 gcgaggcgac gagaccgatc accgcggtga gcagcgccgc atcgtcggcg aagccgagcc
     1441 ccgcgatcat gtctgggatg atgtcgacgg gcaggatgaa ataggcgatg gccgcaagca
     1501 gcatgccgcg cacccgcatc ggcgtcgccg ggtcgagcgc gcagtaatag gcggcgacca
     1561 ggtcgtcgac gaagggaatc atcccggcga accgcttgag cttggcccag aagtcgcgcc
     1621 ggaccttgcg ctcccgctcg gcgaaggcag aaagcgaaag ctgccggccg aaggccgcag
     1681 ccttgagctc gcttgcttcg ttgaaggcca tcatcgtccg cacctcgcaa agggggttca
     1741 acgccaggaa tccctcaagg aaaaataggg tcgccggccc ttccccgcaa gcgtttcgcg
     1801 gaaagcacct gtcgagcccc agcgcagcgt ttcctcgaat ttcacacttt tccgtcaggc
     1861 tcgggcggtc ctggtggcgt gaggtcttgc gttttgctcg aggcgggccg tttgccgata
     1921 ctcgccggtg ccctgcttgc ggcgctggcc gcgctgtatt gcgcgatccg ggtgccggcc
     1981 gacgccatga ccgggcagca agcgccgcct tggaccgtga cgccgctcga cgccacggtc
     2041 gccagcaagc tgcgccagct cgacgctctc cgccgcgaca tggagcaagc gggctgcgaa
     2101 tacgccgccg acgacgccag catcgaggga tgccagcaac tcgacgccac tgcacgccgg
     2161 ctcgaggcgg agatcgagga gctgaaggcg cgcgccggct ggactctgga aaaacaggag
     2221 gcggtggaag ccggcg
//
LOCUS       DATNAE010000298         3323 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_1797451, whole genome shotgun sequence.
ACCESSION   DATNAE010000298 DATNAE010000000
VERSION     DATNAE010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1797451"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..679
                     /locus_tag="VN518_06665"
     CDS             68..679
                     /locus_tag="VN518_06665"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXE04291.1"
                     /translation="MLHPGCHQGPNRTALNRYPEVFAAAAAAAPAARRILSFGCSSGE
                     ECVTLAEYFPKAEIIGADINPVILLQARKHRSDRICFVYASDRILSGLGGFDAIFCMA
                     VLRSAGHYPFEIFEERALFLETLVRPRGLLVIHNSPYRFGDTARKDSYETIPVAAPRD
                     IGSFLPDGVTEVEPDGSIFRKLKVHASRSRDEAGNKSDCPWPA"
     gene            complement(701..1117)
                     /gene="mutT"
                     /locus_tag="VN518_06670"
     CDS             complement(701..1117)
                     /gene="mutT"
                     /locus_tag="VN518_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HXE04292.1"
                     /translation="MEGRTLPLVLVAACVLLDGDGRILIAKRPEGRSLAGLWEFPGGK
                     VELGESPEHAVIRELAEELGIGIAAADLAPLTFASHDYPDFHLLMPLFLCRRWRGTVA
                     PHEGQELLWVKPAELGAYAMPLADEPLKALLPALLK"
     gene            complement(1124..2365)
                     /gene="argJ"
                     /locus_tag="VN518_06675"
     CDS             complement(1124..2365)
                     /gene="argJ"
                     /locus_tag="VN518_06675"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HXE04293.1"
                     /translation="MMGKVSPFAPKVLPHLPAIDGVRFASCAAGIRYPGRTDLLLALF
                     EPGTTVAGVLTKSKTASAPVEWCRSRLEHGMARALVVNSGNANAFTGKRGREAVKLTA
                     EAAAAAAGCLEADVYISSTGVIGEPLDPSKFTSLLAGLAEDAGPERITEAARAIMTTD
                     TYPKLVTRQTEIEGVPVTINGIAKGAGMIAPDMATMLAFLFTDAAIEPRPLHEILAPA
                     VDATFNAITIDGDTSTSDTLLLFATGTAASRGAPHIGLPHDPRLAGFRAALTEVLLDL
                     AHQVVKDGEGATKFVTVRVTGAESATAARRIAFSIANSPLMKTAIAGEDPNWGRVVMA
                     VGKAGEAADRDSLAIWLGDIQVAKEGEVAPSYSEALGAAYMKRDEIAITVDVGVGDAS
                     STVWTCDLTADYIAINADYRS"
     gene            complement(2396..3283)
                     /locus_tag="VN518_06680"
     CDS             complement(2396..3283)
                     /locus_tag="VN518_06680"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HXE04294.1"
                     /translation="MKAWRSLLLVAAFVACVPFTASADDVVVAQINGVDIKQSDLDFA
                     ASEVGAQLANFPPEDRRRMLLQFVMENELMAEAAAKAGLDSGQSFEDRLKYHRRRALR
                     DAYFDKSVRNAVPDEEAKKVYDAKVAGMKPEEEIHARHILVATEAEAKEVKERLQKGE
                     DFATVAKEKSKDPSAEGGDLGFFSRGQMLKPFEDAAFALKEGEISDPVQTQFGWHIIK
                     VEEKRTRPLPTFDQVKDTIISQLSAQKAKQTIKELHDAAKIEIVDPEIKKSMQDAAAR
                     GEGQPEDEPDAGGNVEDNH"
BASE COUNT          567 a         1081 c         1051 g          624 t
ORIGIN      
        1 tgaatcaact tcaagacatc gcgcatttca ttaccttaag cggggcaagt tcgccaaggc
       61 ggtgtcggtg ttacatcctg gttgccacca gggaccgaac cggaccgcgc tcaatcgcta
      121 tcccgaggtg ttcgctgcgg ccgctgcggc cgcaccagcg gcgcgtcgca ttctctcctt
      181 cgggtgctcg agcggagagg aatgcgtcac gctcgccgag tattttccga aggccgagat
      241 catcggcgcc gacatcaatc cggtgatcct gctgcaggcg aggaagcacc gcagcgatcg
      301 aatttgcttc gtctatgcga gcgaccgcat cttgagcgga ttgggcggat tcgatgcgat
      361 cttctgcatg gccgtgttgc gcagcgccgg ccattatccg ttcgagatct tcgaggagcg
      421 cgcactcttc ctcgagacgc tggtacggcc acgcgggctc ttggtgattc acaactcgcc
      481 ctaccgcttc ggcgacaccg cacgcaaaga ctcctacgag actatcccgg tcgccgcacc
      541 acgggacata ggctcgttcc ttcccgacgg cgtcaccgaa gttgagcccg acggcagcat
      601 attccgcaag ctcaaggttc atgcgtcccg cagccgcgat gaagccggca acaagagcga
      661 ctgtccctgg ccggcctaac ttagcgcgaa aagacaagcc tcattttagc agcgccggca
      721 gcagcgcctt cagcggctca tcggccagcg gcatggcgta agcgccaagc tccgcgggct
      781 tcacccacag aagctcctgg ccttcatgcg gagcgaccgt tccccgccag cgccggcaga
      841 ggaaaagcgg catcagcagg tggaagtccg gatagtcgtg gctcgcgaag gtgagcggcg
      901 caagatcggc tgccgcgatg ccgatgccga gctcctcggc gagctcgcga atgacggcat
      961 gctcgggcga ttccccaagc tcgaccttgc ctccggggaa ttcccaaagc ccggcaagcg
     1021 atctgccttc cggcctcttg gcgatcagga tcctgccgtc gccgtcaagc agcacgcacg
     1081 ccgcgaccag cacgagggga agcgtgcggc cttccatggc gggtcacgac cggtagtcgg
     1141 cattgatggc gatgtagtcg gcggtgagat cgcaggtcca tacggttgag gatgcgtcgc
     1201 ccacgccgac atcgacggtg atggcgatct cgtcgcgctt catataggcg gcgccgagcg
     1261 cctcgctgta ggagggcgcc acctcgccct ccttggcgac ctgaatatcg ccaagccaga
     1321 tggcaaggct gtcgcggtcg gcggcttcgc cggccttgcc gacggccatg acgacccggc
     1381 cccaattggg gtcttcgccg gcaatcgcgg tcttcatcag cggtgaattg gcgatggaga
     1441 aggcgattcg tcgcgccgct gtcgcactct ccgcgccggt gaccctcaca gtgacgaact
     1501 tggtcgcccc ctcgccgtct ttcaccacct ggtgcgcaag atcgagaagc acctcggtga
     1561 gcgcggcgcg gaagcccgca agccgcggat cgtgcggcag gccgatatgc ggcgccccac
     1621 gcgacgccgc agtaccggtg gcgaacagga gcagcgtatc gctggtcgag gtgtcgccgt
     1681 cgatggtgat ggcgttgaag gtcgcgtcca cggcgggagc gaggatctca tgcaaaggac
     1741 gaggctcgat agcggcatcg gtgaacagga aggcgagcat ggtcgccata tccggcgcga
     1801 tcatgccggc gcccttggca atgccgttga tggtcaccgg cacgccctcg atctcggtct
     1861 ggcgcgtgac gagcttcgga taggtatccg tggtcatgat ggcgcgcgcg gcttcagtga
     1921 tcctttccgg gccggcatct tcggcaagcc cggcgagcag gctcgtgaat ttggacggat
     1981 cgagcggctc gccgatcacc ccggtcgagg agatatacac gtcggcttcg agacaaccgg
     2041 cggcggctgc ggccgcttcc gccgtgagct tcaccgcctc gcgcccgcgt ttgccggtga
     2101 aggcgttggc gttgccggaa ttgaccacga gcgctcttgc catgccgtgc tcgagccttg
     2161 agcggcacca ctcgaccggc gccgacgcgg tcttcgactt ggtgagcacc ccggcaaccg
     2221 tggtgcccgg ctcgaacagc gcgagcaaga gatcggtacg gcctggatag cggatgcctg
     2281 ccgcgcaact ggcgaagcgc accccgtcaa tggcggggag atgcggcagc actttcggtg
     2341 cgaatggcga gaccttgccc atcaaagcct cgcggccgga gatccgggcg atccctcagt
     2401 gattgtcttc gacgtttccg cccgcgtccg gctcgtcctc cggctggccc tcgcctctgg
     2461 ccgcggcatc ctgcatcgac ttcttgatct caggatcgac gatctcgatc ttggcggcgt
     2521 cgtgcagctc cttgatcgtc tgcttggcct tctgcgccga aagctggctg atgatggtgt
     2581 ccttgacctg atcgaaggtc ggcagcgggc gggtccgctt ctcctcgacc ttgatgatgt
     2641 gccagccgaa ctgcgtctgc accggatcgg agatctcgcc ttccttcaag gcaaaggccg
     2701 cgtcctcgaa cggcttcagc atctggccgc ggctgaagaa cccgagatcg ccgccttcgg
     2761 cgctcggatc tttcgacttc tccttggcca ccgtggcgaa atcctcgccc ttctgtagcc
     2821 gctccttcac ctctttggcc tcggcctcgg tggcgaccag gatgtgacgg gcgtgaattt
     2881 cctcctcagg cttcatgcct gcgaccttgg cgtcgtagac cttcttcgcc tcttcgtcgg
     2941 gcacggcatt gcgcacgctc ttgtcgaaat acgcgtcccg caaggcgcgg cgccggtgat
     3001 atttgagccg gtcctcgaaa ctctgaccgc tgtcgagccc ggccttcgcc gcggcctcgg
     3061 ccatcagctc gttctccatg acgaattgca ggagcatgcg gcggcgatcc tcgggcggaa
     3121 aattggcgag ttgggcgccc acttcggagg ccgcgaaatc gaggtccgat tgcttgatgt
     3181 caacgccgtt gatctgggcg acaacgacat cgtcagctga agcggtgaac ggaacgcaag
     3241 caacaaaggc tgcgacaagc agcaacgagc gccaagcttt cacggagtga gatcctcttc
     3301 ttgtgcccgc ctgatcagcg cct
//
LOCUS       DATNAE010000299        13293 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_1198069, whole genome shotgun sequence.
ACCESSION   DATNAE010000299 DATNAE010000000
VERSION     DATNAE010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 13293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13293
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1198069"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VN518_06685"
     CDS             <1..312
                     /locus_tag="VN518_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012336187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HXE04295.1"
                     /translation="AIQDALKLATDVPLRCCQAAREVIDLAAVASAKGNLGVISDAGV
                     AVLAAYAALRSAALNVYTNVKLISDKAFAEAKLKELETLLSGAEAVTEEAYELVKGKV
                     S"
     gene            360..1553
                     /locus_tag="VN518_06690"
     CDS             360..1553
                     /locus_tag="VN518_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015822351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate--CoA ligase subunit beta"
                     /protein_id="HXE04296.1"
                     /translation="MDVHEYQAKELLAQFGVPVPKGAVAFSPDQAVYAATELGGWNWA
                     VKAQIHAGARGKAGGIKLCKTYHEVREAAADLLGKRLVTIQTGPEGKPVQRVYIEVAE
                     PFEREIYLGYVLDRKTERILVIASKRGGMEIEEIARTEPEAILQVVVEPAVGLQAFQA
                     RQLAFALGLNIKQVQRAVTAIMNAYRAFRDRDAIMLEINPLVVTKDDRVLALDAKMSF
                     DDNALFRRHSVADMHDPSQQDPREAQAAEHNLNYIGLDGEIGCIVNGAGLAMATMDMI
                     KHAGGSPANFLDVGGGASPERIATALKLVLSDAKVKAVLVNIFAGINRCDWVAKGVVQ
                     ACQTVDVKVPLVVRLAGTNVEEGRKILADSGVEMITAETLAEAANKAVAACNAATQEP
                     ARARA"
     gene            1576..2487
                     /gene="sucD"
                     /locus_tag="VN518_06695"
     CDS             1576..2487
                     /gene="sucD"
                     /locus_tag="VN518_06695"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HXE04297.1"
                     /translation="MSILIDEKTPIIVQGMTGDKGSFHTKEMVEYGTNVVGGVTPGKG
                     GTKHQGLPVFNTVKEAVDATGATTSITFVAPAFCADAIMEAADAGIRLICSITDGIPA
                     QDMMRVKRYLRRYPKDKRPMMVGPNCAGIISPDKAMLGIMPGHIYLKGKVGVISRSGT
                     LGYEAASQMKAVGIGITTSVGIGGDPINGSSFLDHLVLFEEDPETEAVLLIGEIGGPQ
                     EAEASAWIKENMRKPVVGFVAGLTAPKGRRMGHAGAIISAAGDSAAEKAAIMASCGLT
                     VAPNPSEFGSTVARVLSGGASRPQAVV"
     gene            2554..5319
                     /locus_tag="VN518_06700"
     CDS             2554..5319
                     /locus_tag="VN518_06700"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020188070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HXE04298.1"
                     /translation="MKDDVREFGDAGGPSASALIAGTSPREAAGLLLGLLLDVARKHQ
                     PEIKPLLKGGASVADFTPELLARALQAQGIWFQLLSIAEQNAAMRRRRQVERERGREE
                     LRGTFSHVLAEAARAGVEPEEIQKLISSLRIRPVLTAHPTESKRVTVLEKYRKTYLLL
                     RELENPRWTERERAALVDELRDQIELVWMTGELHLEKPSVEQEVQRGLHFFDETLFEM
                     APKMLTGFESALKHAYPDTRFDVPPFFQFGSWIGGDRDGNPFVTTSVTRTTLKQNALA
                     SLRHYRARLTELARYLSITERASPVPEGFRAELARELEASGDAVAIRARNPGEAYRQY
                     LTVVLRKLDATIARTEGNGGGEGRPYYANADELILDLRVLENALEEAKLASIATDLVR
                     PVRYAVQLFRFSTVRLDLRENTTRTTDTLQALWRATSGQSGGEAPDPKSEAWKSWLLA
                     ELARPRVGDRVLAGLSPEAEETLAMLRLVPEMRGRLDREAFGSFILSMSRSVADVLAV
                     YLLAKEAGVFLDAAGTEICPLPIVPLFETIDDLRSAPAIMRELLAVPLVRRSTNWQGG
                     VQEVMIGYSDSNKDGGFFASNWELAKAQVKLTKVSDETGVKIAFFHGRGGSVSRGGAP
                     TGRSIAAQPAGSIRGRFRVTEQGEVVSSKYANRGTAFYQMELLAASVFEHALKSEREE
                     ALKPRAEIDDAMEALSGASRAAYTRLVGNPDLVTYFQAASPLEEISLLNIGSRPARRF
                     GAKSLADLRAIPWVFAWAQNRHIITGWYGVGSALKNFIEVRGEEGEPMLARMFKDSRL
                     FRLIMDEVEKTLLIVDLPIARAYSGLVAEAQVRDTIFPMIEAEYRLTAEMVRRVSGEA
                     EIGARFPRHRGVLAERLPMINAVNREQVELLRRFRGASTEGERERYKLALLLSINCIA
                     AGLGATG"
     gene            5345..6319
                     /locus_tag="VN518_06705"
     CDS             5345..6319
                     /locus_tag="VN518_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HXE04299.1"
                     /translation="MSFTLIQQATPRLHRSELAVPGSNPSLFEKAAKGQADIIFLDLE
                     DAVAPDDKEQARKNIIQGLNDLDWGTKTMMIRINGLDTHYAYRDVVDVVENCPRLDMI
                     LIPKTGVPQDVYAIDMLVTQIEQYKKREKKIGFEVLIETALGMANVEAIAQASKRLEA
                     MSFGVADYAASTRARTVGIGGPHPDYGVLTDKDADGKRDYHWGDPWHYALSRMMVACR
                     AYGLRPIDGPFGDFSDPDGYMAAAKRAAVLGFEGKWAIHPSQVELANKVFTPSEAEVT
                     KAKRIVEAMKQAAKEGKGAVSLDGRLIDLASIRQAESLLNKARAAGMA"
     gene            6462..6884
                     /locus_tag="VN518_06710"
     CDS             6462..6884
                     /locus_tag="VN518_06710"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HXE04300.1"
                     /translation="MTITGGCRCGAVRYRIEAEPLFTRHCWCRDCQYISAGAGTVNVF
                     FPSGAVKVEGALADYASKAESGNQMHRHFCAACGTPIFTQSEARRHYIGVRAGSLDDP
                     ELGKPEMAIWTSSAPSWAVFAPGIPVEPKQPAPVKPKG"
     gene            7368..7718
                     /locus_tag="VN518_06715"
     CDS             7368..7718
                     /locus_tag="VN518_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE04301.1"
                     /translation="MTEMMTETDGVGSARDAAALRRREIEGLRRASDLLCLWRVCGNA
                     VCRRARSCRGRAHLCAKRNTGAVPPPVREFFLSFLAAKSVGVSSDDFRGEMDGRDETE
                     AFFAWRRACVERQR"
     gene            complement(7766..8458)
                     /locus_tag="VN518_06720"
     CDS             complement(7766..8458)
                     /locus_tag="VN518_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018429634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HXE04302.1"
                     /translation="MPDTPRVKSFLLAPSEPFPFRAGQHVDVRLTAPDGYQAERSYSI
                     ASAPEQTAAIELAVERLDDGEVSPFFHDVAEVGDAIELRGPIGGHFVWDVAADGGPLL
                     LLGGGSGVVPLMSMLRHRAAVGSRVPALLLFSSRTFEDVIFRDELLALDAKNDGFQLA
                     LALTRAQPRREQDFGRRVDAAMVAELVGRLPEPPEHVFICGGNPFVEAAAQGAIEAGV
                     APSMIRTERYGG"
     gene            complement(8523..9131)
                     /locus_tag="VN518_06725"
     CDS             complement(8523..9131)
                     /locus_tag="VN518_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015950855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HXE04303.1"
                     /translation="MVTRGFTGRQQAPDIAKRLPPGQSLTADFPVLSAGPTPHISLGD
                     WSFTLKVGPRPVAKWNWAEFNALPKTHQIRDIHCVTKWSKLDTPWDGVLVDDVLKAAG
                     IDEPPTKFILAHSFDGYSTNVPVADLIGGKAMIALRYDGKPLPPDHGGPARLFVPHLY
                     FWKSAKWVSGLQFTERDEAGFWELRGYHIYGDPWREQRFTGD"
     gene            9254..9535
                     /locus_tag="VN518_06730"
     CDS             9254..9535
                     /locus_tag="VN518_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE04304.1"
                     /translation="MSYRVALLIVAASMLGFACAARAESVTVTKAVQDACAWEYDKFC
                     NQYGLGSELLDICFKQNARNMTKACVDALVAAGDVSREYVDQQKKLLGR"
     gene            9822..10103
                     /locus_tag="VN518_06735"
     CDS             9822..10103
                     /locus_tag="VN518_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE04305.1"
                     /translation="MLMNSYRVKLLVAFASAACLACAAQGQPITKAVQTACKDDYKQY
                     CGEYGLETSGLRSCMDRNGHNLSKICVNALVKSGEVSQSEVDRRKRAAR"
     gene            complement(10621..11103)
                     /locus_tag="VN518_06740"
     CDS             complement(10621..11103)
                     /locus_tag="VN518_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012557416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEKHLA domain-containing protein"
                     /protein_id="HXE04306.1"
                     /translation="MLAERDMMLAYDSDFFGLLVSSYRRTMGAEPAFFESRLPGQRPS
                     AAWLYDEARHPVLAHNTDPDPRFIYANKAAQACFEYGWDEIVLLPSRLSAEPVDREER
                     QRLLDDVARQGFATGYSGWRIAKSGRRFFIEDGVVWQLIDVDGAVRGQAATFAKWRDA
                     "
     gene            11248..12126
                     /locus_tag="VN518_06745"
     CDS             11248..12126
                     /locus_tag="VN518_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003545036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXE04307.1"
                     /translation="MSKLDARKSGSFKVGGEIEIHRLGFGAMRITGKGIWGPPEDKPE
                     AIRTLKRLPALGIDFVDTADSYGPDVSEQLIREALHPYRGMLIATKAGFQRPGPSVWE
                     MDGRPEWLRKQALKSCKQLGVEQIGLWQLHRIDPKVPRDEQFGAVKSLLDDGIIRHAG
                     LSEVSVADIEDASKMFKVATVQNRYNLVDRGSEDVLDYCEANGIGFIPWFPLAAGDLA
                     KPGSILDGIAKAHDATPGQIALAWVLKRSPVMLPIPGTSKVAHLEENVAAVNITLSDQ
                     EFTALDRQGRKEHRAA"
     gene            12200..>13293
                     /locus_tag="VN518_06750"
     CDS             12200..>13293
                     /locus_tag="VN518_06750"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HXE04308.1"
                     /translation="MGVAAVLREELIFVGTCDIAGHVRGKGFPARELAARKKQGIGWT
                     HTNLMQNAFGVILDTPFSTGGDLMVVPDTSAEVRVDFDDGSVPEHIFLGDIRNTDGSP
                     WECCPREFLRRAIAALDAASGLHLIAAFEQEFVYTGVEERPAHAYSLGAWRRQGEFGE
                     SVVAALRAAGLKPDSFLPEYGPRQYEITIAPQPALKAADQAVIARELARATAFRLGHR
                     VSFSPMPVADGTGNGVHIHFSLHDAEGAAVTYDAKGPLGLSEAAAQFCAGVLHHLPAI
                     TAITAPSPVSYLRLTPNRWAPTEIDIVKQDRGAALRVCPVFAATTPKKIAKAFNLEFR
                     VSDGSASPYMALGAMIFAGADGVARKLVLP"
BASE COUNT         2392 a         4271 c         4406 g         2224 t
ORIGIN      
        1 gccatccagg acgcgcttaa gctcgccacc gacgttccgc ttcgctgctg tcaggccgcg
       61 cgcgaggtta tcgatctcgc cgccgtggcc tcggccaagg gcaatctcgg ggtgatctcc
      121 gacgccgggg tcgccgtgct cgcggcttat gctgcactgc gaagtgccgc gctgaatgtt
      181 tataccaacg tgaaattgat ctctgacaaa gccttcgccg aggctaagct taaggaactt
      241 gagacgctct tgtccggcgc tgaagccgtg accgaggaag cttacgagct ggtgaaggga
      301 aaggtgagct gacgctcgcc tggcttatgc cggccaaaaa ccgaaaggga ggaaggaaag
      361 tggacgtcca cgagtatcaa gccaaggagt tgctcgccca gttcggcgtg ccggtgccga
      421 agggcgccgt tgccttcagc ccagaccagg cggtttatgc cgcaaccgag ctcggcggat
      481 ggaactgggc ggtcaaggcg cagattcatg ccggcgcccg cggcaaggcc ggcggcatca
      541 aattgtgcaa gacctatcac gaggtgcgcg aggcggcggc cgatctgctc ggcaagcggc
      601 tggtgacgat ccagaccggg cccgagggca agccggtgca gcgggtctat atcgaggtgg
      661 ccgagccgtt cgagcgggag atctatctcg gctatgtgct cgaccgcaag accgagcgta
      721 tcctggtcat cgcctcgaag cgcggcggca tggagatcga ggagatcgcg aggaccgagc
      781 ctgaagccat cctccaggtg gtggtcgagc cggctgtcgg cctgcaggct ttccaggccc
      841 gccagcttgc cttcgcgctt ggcctcaaca tcaagcaggt gcagcgcgcg gtgaccgcga
      901 tcatgaacgc ctatcgcgcg ttccgcgacc gcgacgccat catgctcgag atcaatcctc
      961 tggtggtgac caaggacgat cgcgtgctcg cgctcgacgc caagatgtca ttcgacgaca
     1021 acgcgctgtt ccgccgccac agcgtcgccg acatgcacga tccttcgcag caggacccgc
     1081 gcgaggcgca ggccgccgag cacaatctca attatatcgg gctcgacgga gagatcggct
     1141 gcatcgtcaa tggcgcaggg ttggccatgg ccactatgga catgatcaag catgccggcg
     1201 gcagcccagc gaacttcctc gatgtcggcg gcggcgcgtc tcctgagcgc atcgccaccg
     1261 cgctcaagct cgtgctgtcg gacgccaagg tgaaggcggt gctggtcaac atcttcgccg
     1321 gcatcaatcg ctgcgactgg gtggcgaaag gtgtggtgca ggcctgccag acggtcgacg
     1381 tgaaggtgcc gctcgtggtc cggctcgccg gcaccaatgt cgaggagggc cgcaagatcc
     1441 tagccgacag cggggtggag atgatcacgg ccgagacttt ggcggaggcc gcaaacaagg
     1501 ccgtggccgc ctgcaacgcg gcgacgcaag aacctgcaag ggcgagggcc taaagcgccc
     1561 gccgggagtg gaccaatgag cattcttatc gacgaaaaga caccgatcat cgtccagggc
     1621 atgaccggcg acaaaggctc cttccacacc aaggagatgg tggagtacgg aaccaacgtc
     1681 gtgggcgggg tgacgccggg taaaggcggc accaagcatc agggcctgcc ggtgttcaac
     1741 accgtcaagg aagcggtgga cgcgaccggg gccaccacca gcatcacctt cgtggcgccg
     1801 gccttctgcg ccgatgccat catggaggcg gccgatgccg gcatcaggct gatctgctcg
     1861 atcaccgacg gcatcccggc gcaggacatg atgcgggtga agcgctatct ccgccgctac
     1921 cccaaggaca agcggccgat gatggtcggc ccgaactgcg ccggcatcat cagccccgac
     1981 aaggccatgc tcggcatcat gccgggccac atctatctga aaggcaaggt cggggtgatc
     2041 tcgcgctcag gtacgctcgg ctatgaggcc gcctcgcaga tgaaggcggt cggcatcggc
     2101 atcaccacgt cggtcggcat tggcggcgat ccgatcaacg gctcgtcctt cctcgaccat
     2161 ctcgtcctct tcgaggagga tccggagacc gaggcggtgc tgctcatcgg cgagatcggc
     2221 ggtccgcagg aggccgaggc ctcggcctgg atcaaggaga acatgcggaa gccggtggtt
     2281 ggcttcgtcg ccggcctcac cgcgcccaag ggacggcgca tgggccatgc cggcgccatc
     2341 atctcggcgg caggcgacag cgctgccgag aaggccgcga tcatggcatc ttgcgggctc
     2401 accgtggcgc cgaacccgag cgagttcgga tcgaccgtgg cgcgcgtgtt gtccggtggc
     2461 gcgtcgaggc cacaggctgt cgtctgagct gaaggcgcgg cgccgcatag tcatgggcgg
     2521 ggcgatgcgc cccgccggga gtgccgggcc atcatgaagg acgatgtcag agagttcggt
     2581 gatgcgggcg gcccgtccgc atcggctctg atcgccggca cgtcgccgcg tgaagccgct
     2641 gggcttctgc tcgggctcct gctcgacgtg gcgcgcaagc atcagcctga gatcaagccg
     2701 ctgctcaaag gcggtgcatc cgtcgccgat ttcacgcccg agctcctggc gcgcgcgctg
     2761 caggcgcaag gcatctggtt ccagcttctc tccatcgccg agcagaacgc cgccatgcgc
     2821 cgccgccgcc aggtcgagcg cgagcgcggc cgcgaagaat tgcgcggcac gttcagccat
     2881 gtgctggccg aggcggcgcg ggccggcgtc gagcctgagg agattcagaa gctgatctca
     2941 agcttgcgca tccgcccggt gctgaccgcg caccccaccg agtcgaagcg cgtcaccgtg
     3001 ctcgagaagt atcgcaagac ctatctcttg ctgcgcgagc tggaaaatcc gcgctggacc
     3061 gagcgggaac gcgcggcact ggtcgatgag ctgcgcgacc agatcgagct cgtgtggatg
     3121 accggcgagc tgcatctgga gaagccgtcg gtcgagcagg aggtgcagcg cgggctccat
     3181 ttcttcgacg agactctgtt cgagatggcg ccgaagatgc tgaccgggtt tgagtcggcg
     3241 ctgaagcacg cgtatccgga tacgcgcttc gacgtgccgc cgttcttcca gttcggctcg
     3301 tggatcggcg gcgaccgcga cggcaatccc ttcgtcacca cgtcggtgac gcgcaccacg
     3361 ctcaagcaga acgcgctggc ctcgctgcgg cactaccggg cacggctcac cgagcttgcc
     3421 cgctatctgt ccatcaccga gcgcgcctcg ccggtgccgg aaggcttccg cgccgagctt
     3481 gcccgcgagc tggaggcgag cggcgatgcg gttgccatcc gcgcccgcaa tcctggcgag
     3541 gcctatcgcc aatatctcac cgtcgtgctg cgcaagctcg acgccaccat cgcccgcacc
     3601 gagggcaatg gcggaggcga ggggcgtccc tattacgcca atgccgacga actcatcctc
     3661 gatctccgcg tgctggagaa cgcgctggag gaggcgaagc tcgcctcgat cgccaccgat
     3721 ctggtgcggc ccgtccgcta cgccgtgcag ctcttccgct tctcgaccgt gcgcctcgat
     3781 ctccgcgaga acaccacgcg caccaccgat accttgcagg cgttgtggcg cgccacgtca
     3841 gggcaaagcg gcggcgaggc gcctgacccc aagtccgagg cgtggaagtc ttggctcctt
     3901 gccgagcttg cccgcccgcg cgtgggcgac cgcgtgctcg ccggcctgtc gcccgaggca
     3961 gaggagacgc tcgccatgct gcggctcgtg ccggagatgc gcgggcgcct cgaccgcgag
     4021 gccttcggct cattcatcct gtcgatgtcc cgctcggtcg cggacgtgct cgccgtctac
     4081 ctgctcgcca aggaggcggg cgtgtttctc gatgccgccg gcaccgagat ctgcccgctc
     4141 cccatcgtgc cgctgttcga gaccatcgac gatctccgct cagcgcctgc catcatgcgc
     4201 gagctgctcg ccgtcccgct cgtgcgccgc tcgaccaatt ggcagggcgg cgtgcaggag
     4261 gtgatgatcg gctattccga ttcgaacaag gacggaggct tcttcgcctc caactgggag
     4321 ctcgccaagg cgcaagtcaa gctcaccaag gtgagcgacg agacgggcgt gaagatcgcc
     4381 ttcttccacg gccgcggcgg ctcggtctcc agaggcggcg caccgaccgg gcgctccatt
     4441 gccgcccaac cggcgggctc gatccgcggc aggttccgcg tcaccgagca gggcgaggtg
     4501 gtgtcctcca aatatgccaa tcgcggcacg gccttctatc aaatggagct gctcgccgcg
     4561 agcgtgttcg agcatgcgct gaaatccgag cgcgaggagg cgctgaagcc gcgcgccgaa
     4621 atcgacgacg ccatggaagc tttgtcaggc gcctcgcgcg ccgcctacac aaggctcgtc
     4681 ggcaaccccg acctcgtcac ctatttccag gcggcgagcc ccctggaaga gatctctctg
     4741 ctcaatatcg gctcgcggcc ggcgcggcgc ttcggcgcca agagcctcgc ggatctccgc
     4801 gccatccctt gggtgttcgc ctgggcgcag aaccggcaca tcatcaccgg ctggtacggg
     4861 gtgggctcgg cgctcaagaa tttcatcgag gtgcgcggcg aggaaggcga gcccatgctt
     4921 gcccgcatgt tcaaggactc acgcctgttc cgcctgatca tggacgaggt ggagaagacg
     4981 ctgctcatcg tcgatcttcc aatcgcgcgc gcctattccg ggcttgtcgc cgaggcgcaa
     5041 gtgcgcgaca cgatcttccc catgatcgag gctgaatacc ggctcacggc ggagatggtg
     5101 cgaagggtga gcggcgaggc cgagatcggc gcccgcttcc cgcgccatcg cggggtactg
     5161 gccgagcggc tccccatgat caacgcggtc aatcgcgagc aggtcgagct gttgcgccgt
     5221 ttccgcggcg cttcgacgga aggagagcgc gagcgctaca agcttgcttt attgctgtcc
     5281 atcaattgca ttgccgccgg cctcggcgcc acgggttaaa ctgtaacgaa aggaaatcga
     5341 cgacatgagc tttaccctga tccaacaggc gaccccgcgt ctccaccgtt cagagcttgc
     5401 cgtgccgggg tccaacccgt cgctgttcga gaaggcggca aagggccaag ccgacatcat
     5461 cttcctcgac ctggaagacg ccgtcgcccc cgacgacaag gagcaggcgc gcaagaacat
     5521 catccagggc ctcaacgatc tcgattgggg caccaagacc atgatgatcc gcatcaacgg
     5581 cctcgacacc cattacgcct atcgcgacgt ggtggatgtg gtggagaact gcccgcgcct
     5641 cgacatgatc ctgatcccca agaccggcgt gccgcaggac gtctacgcca tcgacatgct
     5701 ggtcacccag atcgagcaat ataagaagcg tgaaaagaag atcggcttcg aggtgctgat
     5761 cgagactgcg ctcggcatgg ccaatgtcga ggcgattgcg caggcctcga agcgcctcga
     5821 ggcgatgagc ttcggcgtcg ccgactatgc tgcctcgacc cgcgcccgca ccgtcggtat
     5881 cggcggcccg cacccggact acggcgtgct caccgacaag gacgccgacg gcaagcgcga
     5941 ctaccactgg ggcgacccct ggcactatgc gctgtcgcgc atgatggtcg cctgccgcgc
     6001 ctacgggctt cgcccgatcg acgggccgtt cggcgatttc tcagaccccg acggctacat
     6061 ggcagcggcg aagcgcgccg cggtgctcgg cttcgagggc aaatgggcga tccatccgag
     6121 ccaggtcgag cttgccaaca aggtgttcac gccgtccgag gccgaggtga ccaaggccaa
     6181 gcgcatcgtc gaggcgatga agcaggcggc gaaggagggc aagggcgcgg tgtctctcga
     6241 cggccggctg atcgaccttg cctcgatccg ccaggccgag tctctgctca acaaggcgcg
     6301 cgccgccggg atggcctaga gcgtcactag accaaagtgc agttgtcatg cccgcgaaag
     6361 cgggcatcca gtaatcaccg gagtcgcgag agttaagacc gaggcgcttt cacttgctcc
     6421 atgcctcgag cggctgcgct acggaaatga gggaggaaca catgaccatc accggaggat
     6481 gccgctgcgg cgcggtgcgc tacaggatcg aggcggagcc gctgttcacc cggcattgct
     6541 ggtgccgaga ctgccaatat atcagcgcag gggccggcac ggtgaacgtg ttcttcccaa
     6601 gcggcgcggt gaaggtcgaa ggcgcgctcg ccgactatgc gagcaaggcc gagagcggca
     6661 accagatgca ccggcacttc tgcgccgcct gcggcacgcc gatattcacc cagtccgaag
     6721 cgcggcgtca ttatatcggg gtgcgcgcag ggagcctcga cgatccggag ctcggcaagc
     6781 cggagatggc gatctggacc tcgagcgcgc cctcttgggc cgtcttcgcg ccaggcatcc
     6841 cggtcgagcc aaaacaaccc gcaccggtaa agccgaaggg gtgaggatgg tccagcgaga
     6901 gtgttgcgct gcaggcttcg gcctactcga gcgcgacgtg actgagaccg gacaaggcga
     6961 tccgtcgccc gagtttcgag cgaagtccca agcgaaagcg ctgcccggcc gtccgtcaaa
     7021 ttaccgcacg tggcgcgccg agaggcgccc gccgtgtccc ccaacggatg cggagccata
     7081 ggtctgcgct tttcggcgcg ccaccccctt cggggggaag cccccttcgg acgaagtccg
     7141 ttcgggccca cttcgtgggt tggggaaagt cagcaaaccc cggatgcgaa cgcatcgcgg
     7201 caacgaagga gcatgtccgt acgtggcgca gcaactgccc gagcacaatg cccccacccc
     7261 agcgtcgcgc tccgcgctca agcagcgaaa gcggtaacgc atcaaaaagc gcgccgccgg
     7321 acctccccgc gagggggagg tttggagcga gagttgaggt gatgacaatg actgagatga
     7381 tgaccgagac ggacggcgtg ggctccgcgc gcgatgcggc agcactgcgc cggcgtgaga
     7441 tcgaaggctt gcgccgggcg agcgatctcc tgtgtctctg gcgcgtgtgc ggcaatgccg
     7501 tctgccgccg ggcccgatcc tgccgcgggc gcgcgcatct ttgtgccaag cgcaatacgg
     7561 gcgcggtacc gccgccggtg cgcgagttct ttttatcgtt cctcgctgcg aagtcggtgg
     7621 gcgtcagctc cgacgacttc cgcggcgaga tggacggcag ggacgagacg gaagctttct
     7681 tcgcctggcg cagggcgtgc gtggagcgtc agcgatgatg catctcgctc acccttcaag
     7741 acgccgcttc ggcactccgc gcgcctcagc cgccgtagcg ctcggtgcgg atcatggagg
     7801 gcgcgacgcc agcctcgatg gcgccttggg cggccgcctc gacgaacgga ttgccgccgc
     7861 agatgaagac gtgctcgggt ggctcgggca gcctgccgac aagctcggcc accatggcgg
     7921 catcgacgcg gcggccgaag tcttgctcac gccgcggctg agcacgggtg agcgcgagcg
     7981 cgagctgaaa gccgtcattc ttggcatcga gcgcgagcag ctcgtcgcga aagatcacgt
     8041 cctcgaaggt gcgcgaggag aacagcaaga gcgccggcac gcgcgatccg acggccgcgc
     8101 ggtggcgcag catcgacatc agcggcacga cgccagagcc gccgccaagc aggagcagcg
     8161 ggccgccgtc tgcagcaacg tcccagacga aatgcccgcc gatcggcccg cgcagctcga
     8221 tggcgtcgcc cacctcggcc acgtcatgga agaagggcga gacctcgccg tcgtcgagcc
     8281 gctctacggc gagctcgatc gctgccgtct gctcgggcgc cgaggcgatg gagtagctgc
     8341 gctcggcctg gtagccgtcc ggcgcggtga gccgcacatc gacatgctgg ccggcgcgga
     8401 agggaaaggg ctccgacggc gcgagcagga agctcttcac ccgtggcgtg tcgggcatga
     8461 tcgcggcgat tttcgcctcc tgccagcgga gctccggctc cggcgccttt gagggaatcg
     8521 cctcagtcac cggtgaagcg ctgctcgcgc cagggatcgc catagatgtg atagccgcgg
     8581 agctcccaga agccggcctc gtcgcgttcg gtgaactgca agccgctcac ccatttcgcc
     8641 gacttccaga aatagagatg cggcacgaag agccgcgccg gcccgccatg gtcgggcggc
     8701 aaaggcttgc cgtcatagcg gagcgcgatc atcgccttgc cgccgatgag atcggcgacc
     8761 ggcacgttgg tggaatagcc gtcgaaggag tgcgccagga tgaacttggt cggcggctcg
     8821 tcgatgccgg cggccttgag cacgtcgtcg accagcacgc cgtcccaggg cgtatcgagc
     8881 ttcgaccatt tggtgacgca atggatgtcg cggatctggt gggtcttcgg caaggcgttg
     8941 aactcggccc agttccactt ggcgaccggc ctcggcccga ccttcagggt gaaggaccaa
     9001 tcgccgaggc tgatatgcgg cgtggggccg gccgacagca cgggaaagtc cgcggtcagc
     9061 gactgcccag gcggcagcct cttggcgatg tcgggcgctt gctgccgtcc ggtaaatccg
     9121 cgcgtgacca tgtccctggt tctcctggtt acccgatatt cgtcaagact gttaacctca
     9181 aaaggctcta agcaggcttg aaactttctg ctagtgtccg cccgattctc cagagtaaca
     9241 ggggggaaat tcaatgtcct atcgcgttgc acttcttatt gtcgccgctt cgatgcttgg
     9301 ctttgcctgt gcggcgcgtg ccgagtcggt caccgtcacc aaggcggtgc aagatgcctg
     9361 cgcctgggag tacgacaagt tctgcaatca gtatggactt ggcagcgagc ttctcgacat
     9421 ctgcttcaag cagaacgccc gtaacatgac caaggcctgc gtcgacgccc tcgtcgcggc
     9481 cggcgacgtc tcgcgggagt atgtcgatca gcagaagaag ctgctcggcc gctagtcacc
     9541 gcaatcgctc ctttatcgcc gggcttgatc cggcgattca atccatcggc gcatcccgct
     9601 gaagccgaac gacatggatg cccgggtcaa gcccgggcat gacgaggggg cattggatcg
     9661 cggtcgagtc gccttcgcct cctggttgac ggcgacggcg tccgcgctgt tgcgcttatg
     9721 caagcaagca tactttcttt aggaggaggc tgcgcaactg cgcgggaatc gcattaagct
     9781 cccccgttct cattgagcgc ttgaatgggg cgggggaggc catgttgatg aattcctatc
     9841 gcgttaagct gcttgtcgct tttgcttcgg ccgcttgctt ggcctgcgcg gcgcaaggcc
     9901 agccgatcac caaggcggtg cagacggcct gcaaggacga ctacaagcaa tattgcggcg
     9961 aatacgggct cgagacctcg ggtctcaggt cgtgcatgga ccggaacggg cacaatctgt
    10021 ccaagatttg cgtgaacgcg ctggtcaagt ccggcgaggt cagccagtca gaggtcgatc
    10081 gccgcaagcg agctgcccgc taacgccaaa actgtgttgt cacggcctgg cttgatcccc
    10141 ggaacaggtc cggggagagc ttccgtccat ccatgtcttc tgctccgaat ctggactttg
    10201 ggagagcttg cgcgctcgcc gacgctcctt gagggcgtag gtctcgccca gcggccggag
    10261 aaataggggc acccgctgga aggcgggtgc ccaatatggc agggggaaga gattggtggt
    10321 catcgcgatc accattgcgg ccgatcggcc gccctcgtcc tagcgctggc tgaaggcgtt
    10381 ggggaactgc tcggccactg cgggcgcggc agcggtggcg gcgaaggtga cgaccaaagc
    10441 gacggcgagg agaatcttgt tcatgagagg tctccgatct ggggcggcct gttgaccgaa
    10501 ggctccaacc ggttggccgt ttgtccgatt gaacaacaga catgtggttg ggggtggccg
    10561 gttcttccaa tagccgcccg ctatcgtttc ggtgcttccg ctgcacgaag gcgaaatgct
    10621 tcaggcgtcg cgccacttgg cgaaggttgc ggcttggccg cgcacggcgc cgtccacatc
    10681 gatcagctgc cacaccacgc cgtcctcgat aaagaagcgg cggcctgact tggcgatgcg
    10741 ccagccggaa tatccggtgg cgaagccttg gcgggcgacg tcatcgagaa gcctctgccg
    10801 ctcctcgcga tcgaccggct cggccgaaag gcgagaggga agcaaaacga tctcgtccca
    10861 gccatattcg aagcaagctt gcgccgcctt gttggcatag atgaagcgcg gatcaggatc
    10921 ggtgttgtgg gcgaggacgg ggtggcgcgc ctcgtcatag agccacgcgg cagaaggtcg
    10981 ctggccgggg aggcgggact cgaagaacgc tggctcagcc cccatggtgc gccggtagct
    11041 cgagaccagc agcccaaaga aatcggaatc gtaagcgagc atcatgtcgc gctcagccaa
    11101 catagccttt ggcctttagt caatcttgcg ggctcagacg ttgaattgcc aagatcgcgc
    11161 ttccggcagg tgcggattgg cgaaagcaga agcaaagtcc ggggcgacgc attatcctca
    11221 acccgaaaca attaggagag tacgaaaatg tccaagctcg acgcgcgcaa gtctgggtcg
    11281 ttcaaggtcg gcggcgagat cgagatccat cgcctcggtt tcggcgccat gcgcatcacc
    11341 ggaaaaggca tttggggtcc gccggaagac aagcccgagg ccatccgcac gctgaagcgc
    11401 ttgcctgcgc tcggcatcga cttcgtcgat accgccgatt cctacgggcc cgacgtgtcc
    11461 gagcaactca tccgcgaagc gctccatcct tatcgcggca tgctgatcgc caccaaggct
    11521 gggttccagc gccccggccc cagcgtatgg gagatggacg ggcggcctga gtggctgcgc
    11581 aagcaagcgc tcaagagctg caagcaactc ggggtggagc agatcgggct ttggcagctg
    11641 caccgcatcg accccaaggt gccgcgcgat gagcagttcg gcgcggtcaa gtcgctgctc
    11701 gatgacggca tcatccgtca tgccggcttg agcgaggttt cggtcgccga catcgaggac
    11761 gcctcaaaaa tgttcaaggt ggcgacggtg cagaaccgct acaacctcgt cgatcgcggc
    11821 agcgaggatg tgctcgatta ttgcgaggcg aacggcatcg gcttcatccc gtggtttccg
    11881 cttgccgccg gcgatctcgc caagccgggc tcgatcctcg atggcatcgc caaggcccat
    11941 gacgcgaccc cgggccaaat cgcgctggcc tgggtgctca agcggagccc ggtcatgctg
    12001 ccgatccccg gcacctcgaa ggtcgcgcat ctcgaagaga atgtcgccgc ggtgaatatc
    12061 accctgtcgg atcaggaatt cactgccctc gaccggcagg ggcgcaagga acatcgcgcg
    12121 gcgtagtcaa ccatgttcgc gcgccaatga gggattggtg agcgaagtga gatagggttg
    12181 cggcaacctg catctgacct tgggggttgc cgccgtgctg cgcgaggagc tgatctttgt
    12241 cgggacctgc gacatcgcag gccatgtgcg cggcaaggga tttccggcgc gcgagctcgc
    12301 cgctcgcaag aagcagggca tcggctggac ccataccaat ctgatgcaga acgcgttcgg
    12361 ggtcattctg gatacgccgt tcagcaccgg cggcgatctc atggtggtgc cggacacgag
    12421 cgccgaggtg cgcgtcgatt tcgacgacgg ttccgtgccc gagcacatct ttctcggcga
    12481 tatccgcaac accgacggct ccccttggga atgctgcccg cgcgagttcc tgcgccgcgc
    12541 catcgccgcg ctcgacgccg cgagcgggct gcacctcatc gccgccttcg agcaggagtt
    12601 cgtctatacc ggagtcgagg agcgtcccgc ccatgcttac agcctcggcg cttggcggcg
    12661 tcagggcgag tttggcgaaa gcgtcgtcgc cgcgcttcgc gccgcagggc tcaagcctga
    12721 ttctttcctg cccgaatatg gcccgcgcca atacgagatc accatcgccc cgcaaccggc
    12781 gctcaaggca gcggatcagg cggtgatcgc gcgcgagctc gcgcgcgcca cagcctttcg
    12841 cctcggccac cgcgtcagct tctcgccgat gccggtggct gacggcacag gcaacggcgt
    12901 gcacatccat ttcagcctgc atgatgccga aggcgctgcc gtcacctatg acgcaaaggg
    12961 gccgctgggc ttaagcgaag ccgctgcgca attttgtgcc ggggtgctgc accatctgcc
    13021 ggcgattact gccatcaccg cgccgtcgcc ggtgtcctat ttgcggctca cgccgaaccg
    13081 ctgggcgccg accgagatcg acatcgtcaa gcaggaccgg ggcgcagcac tccgcgtctg
    13141 tccggtgttc gccgccacga cgccaaagaa gatcgccaag gccttcaacc tcgagttccg
    13201 cgtcagcgac ggctcggcga gtccctatat ggcgcttggc gcgatgatct ttgccggggc
    13261 cgacggcgtc gcgcggaagc ttgtcctgcc gaa
//
LOCUS       DATNAE010000300         3243 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_1200535, whole genome shotgun sequence.
ACCESSION   DATNAE010000300 DATNAE010000000
VERSION     DATNAE010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1200535"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1209)
                     /locus_tag="VN518_06755"
     CDS             complement(<1..1209)
                     /locus_tag="VN518_06755"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001124.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HXE04309.1"
                     /translation="MTLGELMGPEAPLPAPWLQVPIVGLTADSRAVKPGYLFAALPGS
                     RTDGARYIADALARGAAAILMPQGATHALNGTPVVEDTDPRRRLALIAARFFGAQPEV
                     AVAVTGTNGKTSVASFVRQLWEQMGFRAASLGTVGVVGPSGTQTLAHTTPDPIELHRI
                     LANLAKSGVTHLALEASSHGLEQRRVDGLRLAAGAFTNISRDHLDYHSSFEDYFNVKL
                     RLFAELLPQGAGAVIDVDSKAGEQVAALAKSRGLSVMSVGRAGKALRLISAERDGFGQ
                     RLVVEHEGGSETFRLPLVGAFQASNALVAAGLCMATGASPTKLLPLLENLHGARGRLD
                     LAGTTRTGAPIFIDYAHTPDALAKALDALRPYVRNRLLVVFGCGGDRDKGKRRQMGAV
                     AVERADIAIVT"
     gene            complement(1261..2937)
                     /locus_tag="VN518_06760"
     CDS             complement(1261..2937)
                     /locus_tag="VN518_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008195719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HXE04310.1"
                     /translation="MSTAASPAERATLAKSRYRYRLACLAFAVSFLAIAGRLVSLGFA
                     AVEPGAGGLLDISTTVHRPDILDRNGRLLATDIKGATLYADPAKVIDRDELVEQVASV
                     LPDIDTAELREKLKQGRRFVAIKRELTPQQQAEIYELGQPGLGFIEEYRRVYPVGATA
                     CHVVGYVNVDNKGLAGIEKFIDDNPQLAMSAPENAVGGEAVTLSLDLGVQHVVREELV
                     NAMATYRAKAAAGAVIDVASGEVIAMVSLPDYDPNHREEALDKDRLNRMTSGVYELGS
                     VFKVLTVAGALDAGSSTMRSSYDASHPIHVGGFTIEDFHGKKRRLTVPEIFIYSSNIG
                     AAKMALSLGVNRHRAFLEKLGLLSRLSTEIGESAAPIIPSPWQQINTITIAYGHGLSI
                     TPLQLATATLPLVNGGFAVTPTFLPRSRLAGLRQSPSVVKPATSQAMLQLMRLNVLKG
                     TGKRAEAEGYRVGGKTGTAEKVVGRHYEGSELLNSFLSVFPTDAPEYLVLVMLDEPKR
                     VPETNNEATAGVNAAPTVGKIVARIAPILGVAPKLPGDQPKFDAKVLASY"
     gene            complement(2964..>3243)
                     /locus_tag="VN518_06765"
     CDS             complement(2964..>3243)
                     /locus_tag="VN518_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE04311.1"
                     /translation="TLDTVSDNDFERARVEAADDALPAPSGPSTKVKSVAVPGVAAAP
                     TARANLDDDLPVATKTTTVKTVPIKAKPVAQAAPAVIPPPVTVSPPEE"
BASE COUNT          557 a         1007 c         1136 g          543 t
ORIGIN      
        1 ggtgacgatg gcgatgtcgg ctctctccac ggcaaccgcg cccatttgac ggcgcttgcc
       61 cttgtcgcgg tcgccgccgc aaccgaacac caccaacagg cggttgcgca cgtaaggccg
      121 aagggcgtcg agagccttgg cgagtgcatc gggagtatgg gcataatcga tgaagatcgg
      181 agcaccggta cgcgtggtgc cggcaagatc gaggcgcccg cgcgcgccgt gcaggttctc
      241 aagcagcggc aacagctttg ttgggctcgc tccggtggcc atgcacaggc ccgccgcgac
      301 aagcgcgttc gaggcctgga aggcgccgac gagcggcagg cggaaagtct ccgatccgcc
      361 ttcatgctcg accacgaggc gctggccgaa gccgtcccgc tcggcagaaa tgagcctcaa
      421 ggctttcccc gcgcgcccaa ccgacatcac cgagagtccg cgcgacttgg cgagcgccgc
      481 cacctgttcg cccgccttgc tgtccacgtc gatcacggcg cccgcccctt gtggcagcag
      541 ctctgcaaac aggcggagct tcacgttgaa atagtcctcg aagctcgagt gatagtcgag
      601 atggtcgcgc gagatgttgg tgaaggcccc ggccgcaagc cgcaggccgt cgacgcggcg
      661 ctgctcgagc ccatggctcg aagcttcgag cgcaagatgg gtgacgccag acttggcgag
      721 attggcaagg atgcgatgca gctcaatggg gtccggcgta gtgtgggcga gggtttgcgt
      781 gcctgacggt cccaccacgc cgaccgtgcc gaggctcgcg gcgcggaatc ccatctgctc
      841 ccagagctgg cggacgaagg acgcaaccga cgtcttgccg ttggtgccgg tgacggcgac
      901 ggcgacctca ggttgagcac cgaagaagcg cgcggcgatc agcgcaagcc tgcgacgcgg
      961 gtcggtgtcc tcgacgaccg gcgtgccatt gagggcatgt gtggcccctt gcggcatcag
     1021 gatggcggcg gcaccgcgcg ccaacgcgtc ggcgatgtag cgcgccccat cggtgcgcga
     1081 tccgggaagg gctgcgaaca aatagccagg cttgacggcg cggctgtcgg cggtcagtcc
     1141 cacgatcgga acctgcagcc agggggccgg cagcggcgcc tcgggcccca tcaactcacc
     1201 aagggtcatc acctgaacat ccacgcgccc taagcgggcg gccgcgcgac aagcgtccct
     1261 ctaataggag gcgaggactt tcgcgtcaaa cttgggctgg tcgccgggaa gcttgggcgc
     1321 cacgccgaga atgggcgcaa tccgcgccac gatcttgccg acagttggcg ctgcgttcac
     1381 acccgccgtc gcttcgttgt tggtctcagg cacgcgcttc ggctcgtcga gcatgaccag
     1441 cacgagatat tcaggggcgt ctgtgggaaa gaccgagagg aaagagttga gcagttcgga
     1501 gccttcgtag tggcggccta ccaccttctc ggccgtgccg gtcttgccgc cgacgcggta
     1561 gccttccgcc tcggcgcgct tgccagtgcc cttcaacacg ttgaggcgca tgagctggag
     1621 catcgcctgg ctcgtggcgg gcttaaccac gctcgggctt tgccgcaggc ctgccaggcg
     1681 cgagcgcggc aggaaggtcg gcgtcacggc gaagccgcca ttgaccagag gcaaggtcgc
     1741 ggtggcaagc tgcaagggcg tgatcgagag tccatgcccg taagcgatgg tgatggtgtt
     1801 gatttgctgc cagggcgagg gaatgatggg cgcagcactt tcgccgatct ccgtgctgag
     1861 gcggctcagc agtccaagct tctcgaggaa ggcgcggtgg cgattgacgc cgagagagag
     1921 cgccatcttc gccgcaccga tattggatga atagatgaag atctccggga ccgtcaggcg
     1981 gcgcttcttg ccgtggaagt cttcgatggt gaagccgccg acgtgaatcg ggtgactggc
     2041 gtcgtagctt gatcgcattg tagagctgcc ggcatcgagc gcgccggcca cggtcagcac
     2101 cttgaacacc gagccaagct cgtagacacc ggacgtcatg cggttcaggc ggtccttgtc
     2161 gagggcctct tcgcggtgat ttgggtcgta gtcgggcagc gagaccatgg cgatgacctc
     2221 tccgctcgca acgtcgatga ccgcgccggc ggcggccttg gcgcgatagg tggccatagc
     2281 gttgaccagt tcctcgcgca ccacgtgctg cacgccgaga tcgagcgaca gcgtcacggc
     2341 ctctccaccg acggcgttct caggcgccga catggcgagt tgcggattgt cgtcgatgaa
     2401 cttctcgatg ccggcgagac ccttattgtc gacattgacg tagccgacga cgtgacaggc
     2461 ggtggcgccg accggataga cgcggcgata ttcctcgatg aagccgagcc cgggctggcc
     2521 aagctcgtag atctcggcct gctgctgcgg cgtgagctcg cgcttgatgg cgacgaacct
     2581 tctcccctgc ttcagcttct cgcgcagctc ggcagtgtcg atgtcgggga ggacgctcgc
     2641 cacctgctcg accagctcgt cccggtcgat gaccttggcg ggatcggcat agagggtggc
     2701 gcccttgatg tcggtggcaa gaagccggcc gttgcggtcg agaatgtcgg gccggtgcac
     2761 ggtggtggag atgtcgagga ggccccctgc ccccggctcc acggcggcga agccgaggct
     2821 caccagcctc cccgcaatgg cgaggaagct caccgcaaag gcgaggcagg ccaggcgata
     2881 gcggtaacgg ctcttcgcca gagtggcccg ttcggcgggc gaagccgcgg tgctcatatg
     2941 aagatcgcgg gtcaagcctc ggcctattcc tcgggaggcg aaaccgtgac gggcggagga
     3001 atcacggccg gggcggcctg agccacaggc ttggctttga tcggaacggt cttgaccgtg
     3061 gttgtcttgg tggcgaccgg caggtcgtca tcgagattgg cgcgagctgt gggagccgcc
     3121 gcaaccccag gcacggccac ggatttgacc ttggtagaag ggcccgatgg cgccggcaac
     3181 gcatcgtcag cggcctcgac gcgggcgcgc tcgaagtcgt tgtcggaaac ggtgtcgagg
     3241 gtg
//
LOCUS       DATNAE010000301         2451 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_1202826, whole genome shotgun sequence.
ACCESSION   DATNAE010000301 DATNAE010000000
VERSION     DATNAE010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1202826"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..380)
                     /locus_tag="VN518_06770"
                     /pseudo
     CDS             complement(72..380)
                     /locus_tag="VN518_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018327165.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(436..1602)
                     /gene="recF"
                     /locus_tag="VN518_06775"
     CDS             complement(436..1602)
                     /gene="recF"
                     /locus_tag="VN518_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HXE04312.1"
                     /translation="MSKAIAGEAAKTRLYVRTLKLTDFRNYARAELKLDARPVVLIGD
                     NGSGKTNLLEAVSLLGAGTGLRSRPYAELCRKDGAGGFAVAAKVVSREDEVEIGTGLP
                     AGSIEETKGRTVRIAGKVASAGALSEHVKLVWLIPAMDGLFTGSASERRRFLDRLVLA
                     VDPKMRSPQGRYDRAMRQRNRLFQMREGSRALFEGLEEQMAEAGTAIAAARLDAVARL
                     AGLIERMREARGEGPFPWAEIALEGRLEEALSRLPATEVEDEFRALLAQGRERDRAAA
                     RALEGPHLSDLIVIHGPKNAPAAHCSSGEQKALLIGLILAKAELIKELEGASPLILLD
                     EIAAHLDQGRREALFGEISSLGAQAWMTGTDRDLFAPIAENAQIFAVNRGKIGA"
     gene            complement(1626..>2451)
                     /gene="dnaN"
                     /locus_tag="VN518_06780"
     CDS             complement(1626..>2451)
                     /gene="dnaN"
                     /locus_tag="VN518_06780"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HXE04313.1"
                     /translation="AITSGPAQFSLQTLPADDFPALSVDDLGHSFTLSAGDLKRLIEK
                     TRFAISTEETRYYLNGIYLHATQSGGKPMLRAVATDGHRLAQVELPLPSGAKDMPGVI
                     VPRKTVVELARLVENGEGEVRVELSPSKIRVSSPRVVLTSKLIDGTFPDYERVIPQGN
                     DKKMEVDNAAFAQAVDRVSTLSSDKGRAVKLTISDGKLTLSVNNPDSGSATEELPVEY
                     GFDPLEIGFNARYLLDISGQLESGTAEFQLADPGSPTMVRDGKDASALYVLMPMRV"
BASE COUNT          386 a          818 c          805 g          442 t
ORIGIN      
        1 aatcccgaca actcggttac cgtctacgac gacggccgcg gcatcccggt ggatattcac
       61 aagggcgaag gcatctcggc ggccgagacg ccttcgccct tgtggatctc ggtggggatg
      121 ccgcggccgt tatcctcgac cgtgaccgag ccatcggcgt tgagcgtcac gaagatctcg
      181 tcggagaagc cggccagtgc ctcgtcgatg gcgttgtcca ccacctcata gaccatgtga
      241 tgcaggcccg agccgtcatc ggtgtcgccg atatacatgc ccggccgctt gcgcacggcg
      301 tcgaggcctt tcagcacttt gatcgactcg gcgccgtact cggcagcggc ttcggccttg
      361 cgtttggaag ctggtgtcat ggttccctga aggcggttgt tggtcttgtt ggtggggttc
      421 ggagccgcgc gtgaatcagg ccccgatctt accccgattc accgcaaaaa tttgggcgtt
      481 ttcagcgatc ggagcgaaca aatcccggtc ggttccggtc atccaggcct gggcgccaag
      541 gcttgaaatt tcgccaaaaa gcgcctcccg ccggccctgg tccaaatgtg ccgcaatctc
      601 atccaaaagg atcagcgggg aggcgccctc gagctccttg atcagctcgg ctttggcgag
      661 gatgagcccg atcagaagcg ccttttgctc gccggaggag cagtgcgccg cgggggcatt
      721 tttcgggccg tgaattacga ttaaatcgct caaatgcggt ccttcaaggg cccgcgcggc
      781 cgcgcgatcg cgttcgcggc cctgagcgag cagcgcgcga aattcgtcct cgacctccgt
      841 cgccggcaag cgcgacagcg cttcttccag cctgccctcg agcgcgatct ccgcccacgg
      901 gaaggggccc tcgccgcgcg cttcgcgcat ccgctcgatc aatccggcca gccgcgcgac
      961 cgcatcgagc cgggcagcgg cgatggccgt gccggcttcg gccatctgct cctccagccc
     1021 ttcgaacaga gcccggctcc cctcccgcat ctggaacagc cggttccgct gccgcatggc
     1081 gcggtcgtag cggccctgtg ggctgcgcat tttgggatcg acggccagca cgagccggtc
     1141 gaggaagcgc cgccgctccg acgccgagcc ggtaaacagt ccgtccatgg cggggatcag
     1201 ccaaacgagc ttcacatgct cggagagagc gccggcgctc gcgaccttgc cggcgatgcg
     1261 cacggtccgt cccttggttt cctcgatgct gccggcgggg aggccggtgc cgatctccac
     1321 ctcgtcctcg cgcgacacga ccttggcggc gacagcgaag ccgccggcgc cgtccttgcg
     1381 acagagctcg gcataggggc gcgagcgcag gccggtcccg gcgccgagca gcgagaccgc
     1441 ttcgagcaaa ttggtcttgc ccgagccgtt atcgccgatc agcaccaccg gccgggcgtc
     1501 gagcttgagc tcggcccgcg catagttgcg gaaatcggtg agcttgagcg tgcggacgta
     1561 aaggcgcgtc ttcgcggcct cgcccgcgat cgccttgctc acgctgtctc ccttaggacg
     1621 agccctcata cccgcatcgg catgagcacg tagagcgcgg acgcatcctt gccgtcgcgc
     1681 accatggtgg gggagccggg atcggcgagc tggaactcgg ccgtgccgct ttcgagctgt
     1741 cccgagatat cgagcagata gcgggcgttg aagccgatct ccagcggatc gaagccatac
     1801 tcgacgggaa gctcctcggt ggcgctgccg ctgtcgggat tgttgaccga cagcgtgagc
     1861 ttgccgtcgg agatggtgag cttcaccgcc cggcccttgt cgctcgacag cgtcgacacg
     1921 cgatcgacgg cctgggcgaa ggcggcgttg tccacctcca tcttcttgtc gttgccttgc
     1981 gggatcaccc gctcgtaatc ggggaaggtg ccgtcgatca gcttcgaggt cagaactacg
     2041 cgtgggctcg agacgcggat cttcgacggc gacagctcga cgcgcacttc tccttcgccg
     2101 ttctcgacca gccgcgccag ctcgaccacg gtcttgcgcg gcacgatcac cccgggcatg
     2161 tccttggcgc ccgagggaag cggcagctcg acctgggcga gcctgtggcc gtcggtcgcc
     2221 accgcccgca gcatcggctt gccgccgctt tgcgtcgcgt ggagatagat gccgttgaga
     2281 tagtaccggg tctcctcggt cgagatggcg aagcgcgtct tctcgatcag gcgtttcaag
     2341 tcccccgccg acagcgtgaa gctgtgaccg agatcgtcga cgctgagcgc cgggaagtcg
     2401 tccgcgggca gcgtctggag cgagaactgc gccgggccgg aggttatggc a
//
LOCUS       DATNAE010000302         2755 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2401465, whole genome shotgun sequence.
ACCESSION   DATNAE010000302 DATNAE010000000
VERSION     DATNAE010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2401465"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..709)
                     /locus_tag="VN518_06785"
     CDS             complement(56..709)
                     /locus_tag="VN518_06785"
                     /inference="COORDINATES: protein motif:HMM:NF016062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006481 - C-terminal protein methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HXE04314.1"
                     /translation="MAVLDLIGAPQIAALLLLAQRGLEELYSARNTKALIALGAHEVG
                     CGFYPVVAATHLAWIASLFFLVPANAPILWPLIVYYLALQLARYWVIFTLGRFWTHRI
                     FTLPGAPVVARGPYRYLRHPNYAVTIVETFVLPMAFGALALAAIMTALWWTVLAYKMR
                     LEDEEMDVRRDVATVLATVGEGLNTDQAQLQAKATLRNEMSPNRSAPSATGLPPRLR"
     gene            complement(717..1817)
                     /locus_tag="VN518_06790"
     CDS             complement(717..1817)
                     /locus_tag="VN518_06790"
                     /inference="COORDINATES: protein motif:HMM:NF012421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III polyketide synthase"
                     /protein_id="HXE04315.1"
                     /translation="MMLKTTRALRASRRLKADFDVEIAGIATAVPRHALPQADAARRA
                     KRLFPGLADYEGLFRNTGIETRYMCQPAEWYEREHGWEERTEIFVHQALDLLEEVARG
                     AVSEAGIELKDIGAIVVNTITGLSIPSLDAKLMNRLDLPASVERLPVFGLGCGGGVAG
                     LARSARYAQAMPGAHVLFLTVDLCSLCLRVNDPSMAMFVSAALFGDGAAGVVLRNTKG
                     TRQASGGRGGIIATRDHFWRDTEHIMGWDIRHDGFGVVLSPDLPLLLRRNLKPAVQAF
                     LDASGMSLGEFKGFLFHPGGAKVLKTVQSLLELRRDDLAYSWAVLRDFGNMSSPTALF
                     TLDLALKEGASGPHLMAAFGPGFSAYFLAVEL"
     gene            2122..>2755
                     /gene="dapB"
                     /locus_tag="VN518_06795"
     CDS             2122..>2755
                     /gene="dapB"
                     /locus_tag="VN518_06795"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HXE04316.1"
                     /translation="MSEMKLAVLGAAGRMGQTLARAIAATEGCVLAAGIEAKGSPAVG
                     RDLGEVAGLGTLGIPITDDPLPVFAEVHGVLDFTTPKATVAFAGLAAQGRIVDVIGTT
                     GLSADDQAKIEAAARHAAIVKAGNMSLGVNLLAGLTERVARTLGPEFDIEILELHHRH
                     KRDAPSGTALLLGRAAASGRNIALKDHSARARDGDVGPRREGDIGFAALRG"
BASE COUNT          512 a          902 c          905 g          436 t
ORIGIN      
        1 gcctcagggg gctcatggtc tgggcgctga ccgacaatga cagcgcttgc gccttttatc
       61 tcagcctcgg cggcaagccg gtggcggaag gcgccgagcg gttcggcgac atctcgttgc
      121 gcaaggtagc cttcgcctgg agttgagcct ggtcggtgtt caggccttcg ccgacggtcg
      181 cgagaacagt cgcaacgtca cgccgcacat ccatctcttc atcttccaaa cgcatcttgt
      241 aggcgagcac ggtccaccac agcgccgtca tgatcgctgc aagcgcaagc gcgccgaagg
      301 ccatcggcag gacgaaggtc tcgacgatcg tcaccgcata attcgggtgg cgcaggtaac
      361 ggtaaggtcc gcgcgccacc accggggctc caggcagcgt gaagatgcgg tgggtccaaa
      421 agcgcccgag cgtgaaaatg acccaataac gcgcgagctg gagcgcgagg tagtaaacga
      481 tgagcggcca gagaattggc gcgttggcag gaaccaggaa gaacagcgac gcgatccagg
      541 caagatgcgt cgctgccacc accggataaa agccgcaccc aacctcgtgc gcgccgaggg
      601 cgatcagcgc cttcgtattg cgcgccgagt aaagctcttc caggccgcgc tgagcgagca
      661 gcagaagcgc cgcaatctgc ggagcgccga tgaggtcgag gacggccacg cgccactcag
      721 agctcgaccg cgaggaaata ggccgagaat ccgggcccga aggcggccat cagatgcggg
      781 ccgctcgcgc cttccttgag cgccagatcg agggtgaaga gagcggttgg cgaggacatg
      841 ttgccgaagt cgcgcaacac cgcccacgaa taggcaagat cgtcgcgccg gagctcgagc
      901 aggctctgca ccgtcttgag caccttggcg ccgccggggt gaaacagaaa gcccttgaac
      961 tcgccgaggc tcatgccgct cgcgtcgaga aacgcctgca cggcgggctt gagattccgg
     1021 cgcaagagca gcggcaggtc tgggcttagc accacgccga atccgtcatg cctgatgtcc
     1081 cagcccatga tgtgctcggt gtcgcgccag aaatgatcgc gcgtggcgat gatccccccg
     1141 cgcccgcccg atgcctgccg cgtgcccttg gtgttgcgca agactacgcc ggcggcaccg
     1201 tcgccgaaca gcgccgccga cacgaacatg gccatgctcg gatcattgac gcgaaggcag
     1261 aggctgcaaa gatcgacggt gaggaacagc acatgcgcgc ccggcatcgc ttgcgcgtag
     1321 cgcgccgatc gcgcgagccc ggcaacgccg ccgccgcaac cgagcccgaa caccggcaac
     1381 ctctcgaccg atgccggcag atccagccgg ttcatcagct ttgcgtcgag gctcggtatg
     1441 gagaggccgg tgatggtgtt gacgacgatc gcgccgatgt ccttgagctc gatgccagct
     1501 tcactgacgg cgcctcgcgc tacctcctcg agcaaatcga gggcctggtg cacgaagatt
     1561 tcggtccgct cttcccagcc atgctcccgc tcgtaccact cggcgggctg acacatgtag
     1621 cgtgtctcga tcccggtgtt gcggaagaga ccttcatagt cggcaaggcc ggggaacaga
     1681 cgcttggcgc gccgagccgc gtcggcttgc ggaagcgcat gccgagggac ggcggtggcg
     1741 atgcccgcaa tctcgacatc gaagtccgcc ttcaagcgac gggaagcgcg aagcgcgcgc
     1801 gttgtcttca gcatcatgaa ttccccggtg ggcgagcctc tcaggtgggg agtttggacc
     1861 ttcgaacaac gcgcgaacca gcggttgcgc gtcgcaggaa cgtcaattct tcgtgtggtt
     1921 tcggcaacgc gggcaaaatt gcagcaatcg cccgccacct tgcctcccgc aaaatgccgc
     1981 aaagctcttt gccagggagt aaccgcgccg gccgatgtgg cttgtgacaa gcgaagtcac
     2041 ggcctagaag gtcgatggcg cgcgggcgac gaaaggaacc cgcgcgtcaa ccgtctaggc
     2101 ccttttcgtg gttctttggc catgtccgag atgaagctcg ctgtgcttgg cgccgcaggc
     2161 cgcatggggc agacgcttgc ccgggccatt gccgcgaccg aaggctgcgt gctcgccgcc
     2221 ggcatcgagg ccaagggctc gcccgcggtc ggccgcgatt tgggcgaggt ggcagggctc
     2281 gggaccctcg gcatccccat caccgacgat cctctgccgg tcttcgccga ggttcatggc
     2341 gtcctcgatt tcaccacgcc gaaggccacg gtcgcttttg ccgggctcgc cgctcaggga
     2401 cgcattgtcg atgtcatcgg cacgaccggg ctctcggctg acgatcaggc caagatcgag
     2461 gcagctgccc ggcacgcggc gatcgtcaag gccggcaata tgagtctcgg cgtcaatctt
     2521 ctggccggtc tcacggaaag agtggcgcgc acgctcgggc ccgaattcga catcgagatc
     2581 ctcgagctgc accaccgcca caagcgcgac gcgccgtccg gcactgcgct tctgcttggc
     2641 cgggccgccg cttcaggccg caacattgcg ctgaaggacc acagcgcccg cgcccgcgac
     2701 ggcgacgtgg gcccgcgccg cgaaggcgac atcggctttg ccgccttgcg cggcg
//
LOCUS       DATNAE010000303         3392 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_901046, whole genome shotgun sequence.
ACCESSION   DATNAE010000303 DATNAE010000000
VERSION     DATNAE010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_901046"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="VN518_06800"
     CDS             <1..636
                     /locus_tag="VN518_06800"
                     /inference="COORDINATES: protein motif:HMM:NF013624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE04317.1"
                     /translation="AAENSSAWLVEELAGDRQSSQLGCDLHAEVLPTEWAEVARLGPG
                     LVQHHRPRLTLHLGLHKRAQGFRIERSAHNLIDLREDARGAVLDRRTILNHSDRRLDT
                     AIPALQLARHLRTRGLPAVASRSAGTYLCNYLYYLSLHWARGQNGACDVCFVHLPPGP
                     SQGGPLSEAELLRGAELIMRYLLAFALDRDRAETSGREIGPGMRVAEPMTR"
     gene            647..1540
                     /locus_tag="VN518_06805"
     CDS             647..1540
                     /locus_tag="VN518_06805"
                     /inference="COORDINATES: protein motif:HMM:NF024120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HXE04318.1"
                     /translation="MTATRREVILAGIGLGIAFAAGPSFAQTRTFGVVPSGGATDQTE
                     MLQEAVDAAACSGLPLFLPPGIYSTRRLELRSGTHLSGVSGQSIVRCRDGGGLIGIDQ
                     ARDIRLESLVLDGDGKDMGVDGALLAATMVARLQLSNCRFLRSGSSGILVSRCEDVAI
                     IGNIVDKAATGIAVTNAAQGCSRALIQRNLVRNLFFRKVALFHGNGISVDADAVVRGN
                     SVENAPGFGILVGRDLRGVSITDNHIRNAHIGVGIPAEIVMTARLAGNVISNAQNGAI
                     RAMRGPAPIGPDLAVYAGALA"
     gene            complement(1559..2614)
                     /locus_tag="VN518_06810"
     CDS             complement(1559..2614)
                     /locus_tag="VN518_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HXE04319.1"
                     /translation="MQDNPILVEVTRGGLVESCHRGAVAIADADGRVLLGLGDIERPI
                     YPRSAVKALQAIPLIESGAAEAFGLRRAELAIACASHSGDETHVGAVRTLLAKAGLDE
                     SLLACGAHWPIGDRASRELSRLGKSPTPIHNNCSGKHAGMLATAVHLGLDPRGYERPE
                     HPLQQEIRRVISGICDVTLEQDEIGIDGCSVPTFALPLAALATGFARLASGRGLEVTR
                     AEAARRLMEACFAAPDLVAGEGRFDTIVMRGLPGTAFAKGGAEGVHCAALPALGIGVA
                     VKVDDGTKRGAEMALAVLLAKLVPGAEHVLAGQLAGEMRTWRGAKVGTVGPSPALKAA
                     VRALPRQSAAATAAAPR"
     gene            complement(2651..3172)
                     /locus_tag="VN518_06815"
     CDS             complement(2651..3172)
                     /locus_tag="VN518_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019977129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HXE04320.1"
                     /translation="MRQARLDQTDRRILSELQADGRITNVELARKVGISAPPCLRRVR
                     ALEEAGFIRGYAALLDDRALGFDVAAFAMVGLHSQAEADLVAFEQRVRGSPIVRDCYM
                     LSGEIDFMLKCVAPDLTAFQEFIIHELTAAPNVASVKTSLVIRASKHDPGVPIDIVLA
                     TERPARAGERART"
BASE COUNT          592 a         1119 c         1091 g          590 t
ORIGIN      
        1 gccgccgaga attcctcggc ctggcttgtg gaggagctcg cgggagaccg ccaatcctct
       61 caactcggat gcgatcttca tgccgaagtg ctgcccaccg agtgggccga ggttgcgagg
      121 cttgggccag gcttggttca gcaccatcgg ccgcgcctga ccctgcatct cggcctgcat
      181 aagcgcgcgc aagggttccg cattgagcgc tcggcgcaca atctgatcga cctccgggag
      241 gatgcgcgcg gcgccgttct cgacaggcgc acgatcctga atcactccga ccgccgcctg
      301 gacacggcaa tccctgcttt gcaactcgcc aggcatttgc ggacgcgggg acttccggcg
      361 gtcgcttcgc gctcggcggg aacctatctc tgcaactatc tctattatct ctcgcttcac
      421 tgggcgcgcg ggcagaacgg ggcgtgcgac gtctgttttg tgcatctccc gcctgggccc
      481 agccaaggcg gccccttgag cgaggctgag ctattgcgtg gcgccgagct gatcatgcgc
      541 taccttctgg ccttcgcgct ggatcgcgac agagccgaga cctctggccg cgaaatcggc
      601 cccggcatgc gcgtcgcgga gccgatgacg cgctgaacag gagatcatga ccgctacccg
      661 ccgagaggtc atccttgccg gcatcgggct tggcattgct tttgcggccg gcccgagctt
      721 cgcccagacc cgcactttcg gcgtggtgcc gtcaggcggc gcaaccgacc agacggaaat
      781 gctacaggag gccgttgacg ccgccgcctg ctcgggactt cccctgtttc ttccgccagg
      841 gatctattcg acacggcggc ttgagctgag atctggcacg catctcagcg gcgtctcggg
      901 ccaatcgatt gtccgctgcc gcgacggcgg cgggttgatc ggcatcgacc aagcgaggga
      961 cattcgtctc gagagcctcg tgctcgacgg cgacggaaag gacatgggcg tggacggcgc
     1021 gctgcttgca gccaccatgg tcgcgcggct tcagctctca aattgccgct tcctgcgctc
     1081 aggctcgagc ggcatcctcg tctcgcgctg cgaggatgtg gccatcatcg gcaacatcgt
     1141 cgataaggct gcgaccggca tcgccgtgac caacgccgca caaggctgca gtcgcgccct
     1201 cattcaacgg aacctcgtgc gcaacctgtt cttccgcaaa gttgctcttt tccacggtaa
     1261 cggaatttcc gtcgacgccg acgccgtggt gaggggcaac agcgtggaaa acgctccggg
     1321 tttcggcatc cttgtgggtc gcgatctgcg cggtgtaagc atcacggaca atcatatccg
     1381 caacgctcac atcggcgtcg gcatcccggc cgagatcgtc atgaccgcgc ggcttgccgg
     1441 caacgtgatt tcaaatgctc aaaacggcgc gattcgtgcc atgcggggcc ccgccccgat
     1501 cgggcctgac cttgccgtct acgccggcgc attagcctga gggctcagcc ctaaccgatc
     1561 aacgtggcgc tgcggcagta gcggcagcgc tttggcgggg caacgcgcgg accgcggcct
     1621 tgagcgcagg tgacgggcca accgtgccca ccttcgcgcc gcgccaagtg cgcatctctc
     1681 ctgcgagttg gccggcaagg acgtgttcgg ccccaggcac gagcttggcg agcagaacgg
     1741 caagcgccat ctcggcgccc cgcttggtgc cgtcgtcgac cttgacggca accccgatcc
     1801 cgagcgccgg caatgcggcg caatggactc cctccgcgcc acccttggcg aaggcggtgc
     1861 cggggagccc acgcatgacg atggtgtcga agcggccctc gccagcaacg aggtctggcg
     1921 cggcaaaaca agcctccatg agccgccgcg ccgcctcggc tcgcgtgacc tcaagccctc
     1981 tgccgctggc aagcctggcg aagccagtgg ccagggcggc aagtggcaga gcaaaggtcg
     2041 gcaccgagca gccgtcgatg ccgatctcat cctgctccaa cgtaacgtcg caaatccctg
     2101 aaatgactcg cctgatctct tgctgcaggg ggtgctcggg ccgctcatag cctcttggat
     2161 cgagcccgag gtgaactgcg gtcgccagca ttccggcatg tttgcctgag caattgttat
     2221 ggatcggcgt cggactcttg cccaagcggc ttaactcgcg cgacgctcgg tcgccgatcg
     2281 gccaatgcgc gccgcaggca agcagcgact cgtcgagccc agccttggcg agcagcgtac
     2341 gcaccgcgcc gacatgagtc tcatcgccgc tatgcgaagc gcaagcgatg gcgagctccg
     2401 cccgcctaag gccgaaggcc tccgcggcgc cgctctcgat tagcgggatg gcctgcaagg
     2461 ccttgacggc cgagcgcgga tagatcgggc gttcgatgtc gccgagccca agcagcacgc
     2521 gcccgtcagc atcggcgatg gcgacggcgc cgcggtggca gctctccacc agcccgccgc
     2581 gcgtcacttc aaccagaatg ggattgtcct gcacggcttc gctcttgatg gtgacacttg
     2641 gaataaagac ttaggtccgc gcccgttcgc cggcgcgtgc gggccgctcg gtcgccaata
     2701 cgatgtcgat gggcacgcca ggatcgtgtt tcgaggcgcg gataacaaga gaggttttga
     2761 cgctcgcgac attcggcgcg gcggtgagct catgaatgat gaactcctgg aacgcggtga
     2821 gatcgggcgc cacgcatttc agcatgaagt cgatttcgcc ggaaagcatg tagcaatcgc
     2881 gtacgatggg cgagccgcgc acgcgctgct cgaacgcgac aagatcggcc tctgcctggc
     2941 tgtgcagccc caccatggcg aaggccgcga cgtcgaagcc gagcgcgcgg tcgtcgagca
     3001 gcgccgcata gccgcggatg aaccccgctt cctccagcgc gcgaacccgg cgcagacatg
     3061 gcggcgccga gatgcccact ttgcgggcga gctcgacatt ggtgatgcgg ccgtcggcct
     3121 gcagctcact caggatgcgc cggtcggttt gatcgaggcg cgcttggcgc atgggcatgc
     3181 tccctggacg atggcctcgc ggccgcgtta gaagcggcag cgctcaaggc tgaaaagatg
     3241 gcgctcctta tcgcgcaatt ttaggtccaa ttgcgaaaag aatcgaaaag aaattgagcc
     3301 ttcaacattc cggaagttcc agaaacgcag gctctgggcc tgaaaacggc acttggatcg
     3361 tcaccgacgg ctcggtcggc atggaggcgc aa
//
LOCUS       DATNAE010000304         4080 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_1493137, whole genome shotgun sequence.
ACCESSION   DATNAE010000304 DATNAE010000000
VERSION     DATNAE010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4080
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1493137"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="VN518_06820"
     CDS             complement(<1..816)
                     /locus_tag="VN518_06820"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HXE04321.1"
                     /translation="MMQIAPRILLAVSLALVLAFGAGPETRAYDRPEGSSGFTQKTLA
                     KAKRHMVVAANPLAAEAGLATLRKGGSAVDAAIATQMVLNVVEPQSSGLGGGAFLLTW
                     DQATKTLLNIDGRETAPEAATPELFLDAAGKPLPFKQAVESGRSVGVPGVLAALKLAH
                     DKYGKLPWAELFAPAITLARDGFSVSPRLAKLLAESDPASFAPAARTYFFDPAGRPRP
                     EGYMLKNPALASTFEAIARDGPAAFYEGSIARDIAAAVQGDPRGAGSLTAQDLA"
     gene            complement(913..>4080)
                     /locus_tag="VN518_06825"
     CDS             complement(913..>4080)
                     /locus_tag="VN518_06825"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008136646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HXE04322.1"
                     /translation="DFPSSLDFESELHHIKKALTPRTRLYLSASHSYRGDDRARIEAL
                     AQLAARVGLPLVATNAVLYHAAHRRPLQDMLTCIREKCTIHDAGLKLEANAERHLKPP
                     QEMARLFAGYEEALHRTIEIADSCSFSLDELKYEYPDEPVPDGKTPQSHLEDLTWEGA
                     AWRFPGDIPEKVRDTLEKELALIEELDYARYFLTVHDIVHYARHLDILCQGRGSAANS
                     AVCYCLAITNVDPTEIDLLFERFVSPERKEPPDIDVDFEHERREEVIQYIYGRYGRDR
                     AGIAATVISYRGRSAVREVGKALGLSEDTVAALATTIWGLSNSALPEEYVRQAGLDPS
                     DPLLARCLELTQELLGFPRHLSQHVGGFVLTRGPLSEVVPIGNAAMEDRTFIEWDKDD
                     LDALGLLKVDVLGLGMLTCIRKGFALLKEHYGRDLSLGTVPPDDPAVYDMLCQADSVG
                     LFQVESRAQMNMLPRLKPRKFYDLVIEVAIVRPGPIQGDMVHPYLRRRCGDEAVEFPS
                     PHPDHGPADELKQVLGKTLGVPLFQEQAMRLAMVAAKFSGPEANELRRAMATFRRRGT
                     IDRLHEKMVSRMTARGYPQEFAERCFNQIKGFGEYGFPESHAASFAHLVYVSAWLKCH
                     YPAAFAAALLNSQPMGFYAPAQIVRCARDHGVEGREVDVIHSLWDCTLEPASRGEFAL
                     RLGMRQVDGLREDDVKAIVSIRDGIPTELNPHLFAPPLATRSIPPLGEGGSAEGRAGW
                     GEYPGVEEIPPPGAPTRADLPTRGRYRASGNAANLFRDVRDLWRRSGVGRATLERLAA
                     ADAFRSLGHDRRQALWEVRGLPKELPLPLFDHAAASEAGAEPPVTLPIMPLSEHVVND
                     YRTLRLSLKAHPMSFLRARIASEGILSCADLKRTRDGARVSVAGVVLVRQRPGSAAGV
                     LFMTIEDETGVANSVIWPKVLERMRKVVMGARLVVVHGRVQRHEDIIHVVAERLEDRS
                     DWLRLLDEEADSMSVPVANADEVKRPDPGCWRSPSDEEQTPFPVAPADHVKRPLAPRE
                     KSHPRWHPRRHPRAERIIPKSRDFH"
BASE COUNT          719 a         1223 c         1447 g          691 t
ORIGIN      
        1 cgcaagatcc tgcgccgtga gagagcccgc cccgcgcgga tcgccttgta cggcggccgc
       61 gatgtcgcgg gcgatgctgc cctcatagaa ggcggcggga ccgtctctgg cgatcgcctc
      121 gaaagtggaa gcgagggctg ggttcttcag catgtagcct tcaggccgcg gccggccggc
      181 gggatcgaag aaataggtgc gggccgcggg ggcgaagctt gccggatcgg actcggcgag
      241 aagcttggcg aggcgggggg agaccgagaa gccatcgcgc gccagcgtga tagcgggtgc
      301 gaacagctcg gcccatggca gcttgccgta tttgtcgtgc gcgagcttga gcgctgcgag
      361 cacgccgggg acgccgaccg agcgtccgct ctccacggct tgcttgaagg gaagcggctt
      421 gccggcagca tcgaggaaca gctcgggcgt cgccgcttca ggcgccgtct cgcgaccgtc
      481 gatgttcagc aaggtcttgg tggcttgatc ccaggtcagg agaaacgcac cgccgcccag
      541 tcccgaggat tgcggctcga cgacattgag caccatttgg gtggcaatgg cggcgtcgac
      601 ggcgctgccg cctttgcgca aggtcgcaag gccggcttcg gcggcgagcg gattcgccgc
      661 caccaccatg tgccgcttcg ctttggcgag cgtcttctga gtgaagccgc tcgagccttc
      721 tgggcggtca taggctctgg tctccgggcc tgcgccgaag gcgaggacaa gggccagcga
      781 aacggcaagc aaaatgcgtg gagcgatctg catcatgcgg gcagcatagc gggtttcaga
      841 aaggtggcta cctacctctc ttttctttga tttgaagtga gagcaagcct ccggtggggg
      901 agaagtgaaa ggctaatgga agtcgcgaga tttggggatg atgcgctcgg cgcgggggtg
      961 gcggcgcgga tgccagcggg gatgtgattt ctcgcgtggg gcaagcggcc gcttgacgtg
     1021 atcggccggc gcaacgggaa aaggcgtttg ctcctcgtcc gaaggcgagc gccagcatcc
     1081 agggtcgggg cgcttcacct cgtcggcatt ggcgaccgga acgctcatgc tgtcggcttc
     1141 ctcgtcgagg aggcggagcc aatcgcttcg atcctcaagc cgttcggcca cgacatgaat
     1201 aatgtcctcg tgccgctgta cgcggccatg cactacgacg agccgcgcgc ccatcaccac
     1261 tttgcgcatc cgctccagca ccttcggcca gatcaccgag ttggcgacgc ccgtctcgtc
     1321 ctcgatggtc atgaacaaaa cgcctgccgc cgagcctggg cgctggcgca ccagcaccac
     1381 gcccgcgacg ctcacgcgtg cgccgtcgcg ggtgcgcttg agatcggcgc aagaaagaat
     1441 gccttcgctc gcgatgcgcg cgcggaggaa gctcatggga tgggctttga gcgaaagacg
     1501 caaggtgcgg tagtcgttaa ccacatgctc ggagagcggc atgatgggaa gcgtgactgg
     1561 aggctcggcc ccggcctcgc tcgccgcggc gtgatcgaac aggggcagcg gcagctcctt
     1621 cggcaggccg cgcacctccc atagcgcctg gcggcgatcg tggccaagcg agcggaaggc
     1681 atcagccgcg gcaaggcgct cgagcgtggc gcggccgacg ccgctccgcc gccacagatc
     1741 gcggacgtcg cggaaaagat ttgcagcgtt tccgcttgct ctatacctcc ccctcgtggg
     1801 gaggtcggcg cgcgtcggcg cgccgggtgg ggggatttct tcgacgccgg ggtattcacc
     1861 ccacccggct cggccttcgg ccgatccacc ctccccaagg ggagggatgg agcgtgtggc
     1921 gaggggcggg gcaaacaggt gagggttcag ctcggtagga atgccgtcgc ggatcgagac
     1981 gatcgctttc acgtcgtcct cgcgaaggcc atccacctgc cgcatgccga gacgcagcgc
     2041 gaattcaccc cgcgaagcag gctccagcgt gcagtcccac aggctgtgaa tgacgtcgac
     2101 ctcgcgcccc tcgacgccat ggtcgcgggc gcagcgcacg atttgcgccg gcgcgtagaa
     2161 gcccatgggc tgggagttga gcagcgccgc ggcgaaggca gcgggataat ggcatttgag
     2221 ccaggcagag acatagacga gatgggcgaa gctcgcggcg tggctctcgg ggaagccata
     2281 ttcgccgaag cccttgatct ggttgaagca gcgctcggcg aactcttgcg gatagccgcg
     2341 cgccgtcatg cggctcacca tcttctcatg cagccggtcg atggtgccgc ggcggcggaa
     2401 ggtggccatg gcgcggcgaa gctcgttcgc ctcggggcct gagaatttcg ctgccaccat
     2461 ggcaaggcgc atggcctgct cctggaacag cggcacgccg agggtcttgc cgagcacctg
     2521 cttcagctcg tcggcaggac catggtcggg atgcggcgag gggaattcga ccgcctcgtc
     2581 gccgcagcgg cggcgcaaat aagggtgcac catgtcgccc tggatgggac ctgggcggac
     2641 gatcgccacc tcgatgacga ggtcgtagaa ttttcggggt tttagccgcg gcagcatgtt
     2701 catctgcgcc cggctctcga cctgaaacaa gccaaccgag tcggcctggc acagcatgtc
     2761 gtacacggct ggatcgtctg gcggcacggt gccgagggag agatcgcggc cgtaatgctc
     2821 cttgagcagc gcgaagccct tgcggataca ggtgagcatg ccgaggccga gcacatcgac
     2881 cttgagcagc ccgagcgcat cgagatcgtc cttgtcccat tcgatgaagg tgcggtcctc
     2941 catggctgcg ttgccgatgg gcacgacctc ggagagcggc ccgcgggtca gcacgaagcc
     3001 gccgacatgc tgggagagat ggcgggggaa gccgagcagc tcttgcgtca gctcgagaca
     3061 gcgggcgagc agcgggtctg acggatcgag gccagcttgg cggacatact cctcaggcag
     3121 ggcggagttg ctcaggcccc agatggtggt ggcaagcgcg gccacagtat cctctgaaag
     3181 gcccaacgcc ttgccgacct cgcgcaccgc cgagcggcca cgataggaga tcacggtggc
     3241 ggcaatgccg gcgcggtcgc ggccgtagcg gccatagata tattggatca cctcctcgcg
     3301 ccgctcatgc tcgaaatcga cgtcgatgtc gggcggctcc tttcgctcgg gcgagacgaa
     3361 gcgctcgaac aggagatcga tctcggtcgg gtcgacattg gtaatggcga ggcagtagca
     3421 gacggcggag ttcgccgccg agccgcgccc ctggcagaga atgtcgaggt gccgcgcgta
     3481 atggacgatg tcgtgcacgg tgaggaaata gcgcgcgtag tcgagctcct cgatcagcgc
     3541 gagctccttc tctagcgtat cgcgcacctt ctctgggatg tcgccgggga agcgccaggc
     3601 ggcgccctcc caggtgagat cttcgagatg cgattgcggg gtcttgccgt caggcaccgg
     3661 ctcgtcggga tattcgtatt tcaactcatc gagagagaag ctgcaggaat cggcgatctc
     3721 gatggtgcgg tgaagcgcct cttcatatcc ggcgaacagg cgcgccatct cctgcggagg
     3781 cttgagatgc cgctcggcat tggcctcgag cttcagccct gcgtcatgga tcgtgcattt
     3841 ctcgcgaatg caggtgagca tgtcctgcag gggacggcgg tgggctgcgt gatagagcac
     3901 ggcattggtg gcgacgaggg ggaggccaac gcgtgccgcc agttgcgcaa gtgcttcgat
     3961 gcgggcgcga tcgtcgccgc gataggagtg gctagccgag aggtagagcc gtgttctggg
     4021 cgtaagcgct ttcttgatgt gatgaagctc ggattcaaaa tcgagagaag agggaaaatc
//
LOCUS       DATNAE010000305         2813 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3307102, whole genome shotgun sequence.
ACCESSION   DATNAE010000305 DATNAE010000000
VERSION     DATNAE010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3307102"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="VN518_06830"
     CDS             complement(<1..307)
                     /locus_tag="VN518_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE04323.1"
                     /translation="MTVAVPTPIRRYFVRLGVSMSVYVAALLAGTYLVQHALIRGPIL
                     WVAALLPGLALVGAFYAIGMLLVETKDEFLRMLLVRQVLYASVLAMSLATVWGFLENF
                     "
     gene            complement(304..477)
                     /locus_tag="VN518_06835"
     CDS             complement(304..477)
                     /locus_tag="VN518_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE04324.1"
                     /translation="MRAVTHALCWAIMLLAAAAAARFGVMDKGSATTLLIVLPIAAWT
                     ALTGRGCVLRRRA"
     gene            702..2537
                     /locus_tag="VN518_06840"
     CDS             702..2537
                     /locus_tag="VN518_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXE04325.1"
                     /translation="MDTLRGNFEALDDSREQEAGEDDSAREAPPLGIGQDERRMQVRA
                     YNHWAGLLEDRNFPSIDDLEPDNLPDFGPFSVLLDFSVGIENPGVAYLGNQLAHECDI
                     SVPDLRTLSDVPSRSLLSRITDHYMQILANQAPIGFEAEFVNQRGATILYRGILLPYS
                     SNDETIDFIYGVINWKELADQRTSDELLLQVDQALETKPEASSKRADDTPLTDWADGP
                     GTTTVANDIDDEPLELSLVEDAPVAIYETEMEEAVEDEDLSAGFAEGLPAFSGRAEDY
                     LDDEECEEEDEDEDGVADGLPKPKFGSLMSIGGGRGVPTEVADDGDGLDEIAPYAPGS
                     ADDGAIEMADVEYADDAVMSLGGFELSANEATGDFSDFDDADHGSMGNAESVESGEEE
                     TLAAIPAGGAIRLAPLEPSDMALGDWLTSAREMAAQARTSEDRTRHALYDAIGRAYDF
                     SLAAAQSPEDFEELVSDSGLSVQDRAPMTPVVKLVFGADYDKTRLTEYAAALSHAHRL
                     ELARGSLGDFLRGAEGGLKGVVRAERQLRRADSGKADPAADSRELLAAKLREMPAVAL
                     ADLDRYGSEFGLVMIRRSEDGDLVIIGEVPEDATLIEKAAKKLVG"
BASE COUNT          546 a          907 c          918 g          442 t
ORIGIN      
        1 cgaaattctc cagaaagccc catacggtgg caagcgacat ggccagcacg cttgcataaa
       61 gcacctgccg caccagcagc atgcgcagga actcatcctt cgtctccaca agcagcatgc
      121 cgatcgcgta gaacgcgccg accagcgcca atcccggcaa cagggcggca acccatagga
      181 tcggcccacg gatgagcgca tgctgcacaa gataagtccc ggcgagcagc gcggcgacat
      241 aaaccgacat cgataccccg agccggacaa agtagcgccg gatcggcgtt ggcacggcca
      301 cagtcatgcc ctccgccgca gtacgcagcc gcgtcccgtg agcgcagtcc aggccgcaat
      361 cggcaggacg atgagcaaag tcgtggccga tcctttgtcc atgacgccaa aacgcgcggc
      421 cgccgccgcg gcgaggagca tgatcgccca gcaaagggca tgagttaccg ctctcatagt
      481 caagcctcct tgaagatatg acaagtgtgc tatacactat tcctgggagt gtcaagcgac
      541 gttgacacca cgtttgggcc gccgccgcgc ttgacgtcgt ggcgctgcgg cgccgaggag
      601 gccattgcga aaaagcgcgt atggcttttg gaacgggttt aacggttaat ttaccacgcc
      661 gggcgacaag ggcgcgtgac tggaaaccgg gggcatgccc aatggacacg ctgaggggca
      721 atttcgaagc gctcgacgat tcgcgcgagc aggaagcggg cgaagacgat tctgcccgcg
      781 aagcgcctcc gctcggtatc gggcaggacg agcggcgcat gcaggtgcgc gcctacaatc
      841 attgggcggg cctgctcgag gatcgcaact tcccctcgat cgacgatctc gagccggaca
      901 acctgccgga cttcggcccg ttttcggtgc tgctcgattt cagcgtcggg atagagaacc
      961 ccggcgtagc gtatctggga aaccagctgg cgcatgaatg cgacatctcg gttcccgacc
     1021 tcaggacgct gtccgatgtg ccaagccgct cgctgctcag ccggatcacc gatcactaca
     1081 tgcagatcct cgccaaccag gcgccgatcg ggtttgaggc cgagttcgtg aaccagcgtg
     1141 gcgcgaccat actctatcgc ggcatactgc tgccttactc gagcaacgac gagacgatcg
     1201 atttcattta cggcgtgatc aactggaagg agctggccga ccagcgcacc tccgacgagt
     1261 tgctgcttca ggtcgatcag gcactcgaga cgaagcccga agcttcgtcc aagcgcgccg
     1321 atgacacgcc cttgaccgac tgggccgatg gtcccgggac cacgaccgtc gccaacgaca
     1381 tcgacgatga accgctcgag ctttcgcttg tcgaagacgc gcccgtggca atctacgaga
     1441 ctgaaatgga ggaagcggtg gaagacgaag acctctccgc cggtttcgcc gaggggctgc
     1501 cggcgttttc tggccgcgcc gaggattacc tcgacgacga ggaatgcgag gaagaagacg
     1561 aggacgagga cggggtggcc gacggccttc cgaagcccaa gtttggctcg ctcatgtcga
     1621 tcggtggggg ccgcggcgtc ccgacggaag tggccgatga tggcgacggc ctcgatgaga
     1681 tcgcgcctta cgcgccaggc tcggccgacg atggcgccat cgaaatggcc gacgtcgaat
     1741 atgccgacga cgccgtcatg tcgctcggcg gcttcgagct gagcgcgaac gaggccaccg
     1801 gggacttcag cgacttcgac gacgccgatc acggctcgat ggggaacgcc gagagcgtcg
     1861 agtccggcga agaggaaacg ctggccgcca ttccggcggg aggcgcaatc cgcctcgcgc
     1921 cgctcgagcc gagcgacatg gcgctcggcg actggctcac ctctgcgcgc gagatggcgg
     1981 cgcaggcccg caccagcgag gatcggacgc gccacgcgct ctatgacgcg atcggccgcg
     2041 cttacgattt ttctctcgcc gcagcccaaa gccccgagga tttcgaggag ctggtgagcg
     2101 attcgggcct ttccgtgcag gaccgcgcgc cgatgacccc ggtggtcaag ctcgtcttcg
     2161 gggcggatta cgacaagacc cgcctgacgg aatatgccgc ggcgctgagc cacgcgcacc
     2221 ggctcgagct ggcgcgcggc tcgctcggtg acttcctccg cggcgccgag ggcggtctca
     2281 agggcgttgt gcgggccgag cgccagctgc gccgggccga cagcggcaaa gccgatccgg
     2341 cggcggactc gcgcgagttg ctcgccgcca agctgcgcga gatgccggcc gtcgcactgg
     2401 ccgatctcga tcgctatggc agcgaattcg gattggtcat gatccggcgc agcgaagacg
     2461 gtgacctggt cattatcggc gaagtgcccg aagacgcgac cttgatcgag aaagccgcca
     2521 agaagctggt cggctgacgc gccacgcctg tcaaagccgc cgccacgggt gtagcgtcgg
     2581 cggaatgcaa gctcccgcaa tcacggcgcg cgctgcctcg acttcttcag ccggggttca
     2641 gcgcttgagc ggctagaagg cgcgccgatg cgctttctcg cccgctcgct cgccttcctc
     2701 gcggcgctcg ccctgtgcgc gcaacaagcc gccgcgcagg atatcagcat cttgcgcgat
     2761 gccgagacgg aacgggtgct caaggatatg gtcaatccgc tcgcggtggc ggc
//
LOCUS       DATNAE010000306         2717 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_2407113, whole genome shotgun sequence.
ACCESSION   DATNAE010000306 DATNAE010000000
VERSION     DATNAE010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2407113"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="VN518_06845"
     CDS             <1..612
                     /locus_tag="VN518_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012091914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HXE04326.1"
                     /translation="QRRAPIYDKSQDGHYNLISALHKSVRGSDPDASLYWLARMLAGG
                     EDPLYIARRLVRMAIEDIGLADPNALVQANAAKEVYDFLGSPEGELALAQVTIYLALA
                     PKSNAAYTAFDAASRAADEGGSLAPPKHILNAPTKLMKQEGYGRGYAYDHNEDEAFSG
                     QNYFPDGMKRQSFYAPTSFGYERGLQERLERFARLRAERSKKR"
     gene            complement(616..957)
                     /locus_tag="VN518_06850"
     CDS             complement(616..957)
                     /locus_tag="VN518_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HXE04327.1"
                     /translation="MALSRDAVLLRIFIGEDDKADHRPLYEAIVLKAREMHLAGATVL
                     RGPMGFGHSSVLHTTKIVRLSQDLPLVIEIVDSEEKINGFVDAIEGMMGSALVTTEKV
                     KVIQYGTKRLD"
     gene            complement(960..1358)
                     /gene="crcB"
                     /locus_tag="VN518_06855"
     CDS             complement(960..1358)
                     /gene="crcB"
                     /locus_tag="VN518_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HXE04328.1"
                     /translation="MAAYLWIAIGSALGGMARYWCSGVAARLIGETFPWGTIVVNIAG
                     SFIIGFFATLTGPDGRIFADTLTRQFVMIGILGGYTTFSSFSLQTLNLVQDGEWWLAG
                     ANVALSVLACLVAVWLGFVLAASINSLKWI"
     regulatory      complement(1441..1499)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            1580..2578
                     /locus_tag="VN518_06860"
     CDS             1580..2578
                     /locus_tag="VN518_06860"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HXE04329.1"
                     /translation="MTQRSPVETRTVEASEDGMRLDRWFRSHYADLPHSRLEKLLRTG
                     QVRVDGGRAKASTRLAAGQSVRIPPLPAAAPAPSPAPKALSKADRAFLASITLYEDDD
                     LLVLNKPPGIAVQGGTKTTHHLDRLLEGLGVSPKTRPRLVHRLDRDTSGVLVVAKRRE
                     VAAKLGRAFQTRSVRKIYWALVKGVPKPPQGKVDAALVKAAGPEGDRVRKARPGEQEV
                     AQSAVTYYAAVDRAGQEVAWLSLKPVTGRQHQLRAHMAIIGHPILGDEKYQGDRDLPE
                     GIENKLNLHARRISFPHPSGEGVVDVTAPLPDHMLASFAAFGFAEADATDAGDSAL"
BASE COUNT          509 a          879 c          876 g          453 t
ORIGIN      
        1 cagcggcgcg ccccgatcta cgacaaatct caggacggcc attacaatct gatcagcgcg
       61 ctgcacaaat cggtgcgcgg ctccgacccc gacgcctcgc tctattggct cgcgcgcatg
      121 cttgccggcg gcgaggaccc actctacatc gcgcggcggc tcgtgcgcat ggcgatcgaa
      181 gacatcggcc tcgccgatcc caatgccctc gttcaggcga acgccgccaa ggaggtctac
      241 gactttctcg gctcgccgga aggcgagctt gcgcttgctc aagtcaccat ctatctcgca
      301 ctcgccccga aatcgaatgc cgcttacacg gccttcgacg cggcgtcgcg cgcggccgac
      361 gagggcggat cgttggcgcc gcccaagcac attctcaacg cgccgaccaa gctgatgaag
      421 caagaaggtt atggccgagg ctatgcctac gaccataacg aggacgaggc cttctcgggc
      481 cagaactact tccccgacgg catgaagcgg cagagcttct atgcgccgac ctcattcggc
      541 tacgagcggg ggctgcaaga gcggctcgag cgcttcgcta ggctgcgcgc ggagcggagc
      601 aagaagcgct gacggctagt cgagccgctt ggtgccgtat tggatgacct tgaccttctc
      661 ggtggtcacc agcgcgctcc ccatcatgcc ttcaatcgcg tcgacgaagc cgttgatctt
      721 ctcctcgctg tcgacgatct cgatgacgag cggcaggtcc tgcgacaggc ggacgatctt
      781 ggtggtgtgc agcacgctcg aatgaccgaa gcccatcgga ccgcgcaaga cggtggcgcc
      841 ggcgaggtgc atctcgcggg ccttcagcac gatcgcctcg tagagcggcc tgtgatcagc
      901 cttgtcgtcc tcgccgatga agatgcgcaa cagaacggcg tcgcgggaga gcgccatggt
      961 cagatccact tcaacgagtt gatcgaggct gcgagcacga agcctaacca cactgcgaca
     1021 aggcaggcga gaacggaaag ggcgacgtta gcgccggcca gccaccattc cccatcctgc
     1081 accaggttta gggtttgcag gctgaaggag gagaaggtgg tatagccgcc gagaatgccg
     1141 atcatgacga attggcgggt gagcgtatcg gcaaagatgc ggccgtcagg gccggtgagc
     1201 gtggcgaaga agccgatgat aaaggagccg gcgatgttca cgacgattgt gccccaggga
     1261 aaggtctcgc cgatcaggcg cgcggcgaca ccagagcacc agtagcgcgc catgccgccg
     1321 agcgcgctgc caattgcaat ccacagatag gctgccatct attgtcccat gcctttggtc
     1381 tacatagcaa aacccgcctc gtggcgggtt ttgcgactta agctcccacc gcgtgcagag
     1441 gatgcggtag gagtcattag ccattggcgg ttcgggggga ctccatcccc ttcaggactg
     1501 ttggcggcaa tgtaacccgg aattcgcacg gtgcaagtgc cgtcgccgtt ggccttaatc
     1561 gtcctttacc tgaggttcga tgacgcagag atcgccggtc gaaacgcgga cagtggaggc
     1621 gagcgaggac ggcatgcgcc tcgaccgctg gttccgcagc cattacgccg acttgccgca
     1681 cagccggctc gagaagctgc tccgcaccgg ccaggtacgc gtcgatggcg gccgcgccaa
     1741 ggcgagtacg cggctcgccg ccgggcagag cgtgcgtatc cccccgctgc cggccgcagc
     1801 gcctgcgcct tcgcctgcgc cgaaggcgct gtccaaggca gaccgggctt tcctcgcctc
     1861 catcacgctt tatgaggacg acgatctctt ggtgctcaac aagccgccgg ggatcgccgt
     1921 gcagggtgga accaagacca cgcaccatct cgaccggctg ctcgaggggt taggcgtcag
     1981 cccgaagaca cgtccgcgtc tggtgcaccg gctcgaccgc gacacctccg gcgtgctcgt
     2041 ggtggccaag cgccgcgagg tggcggcgaa gctcggccgc gccttccaga cccgctctgt
     2101 gcgcaagatc tattgggcgc tggtcaaggg cgtgccgaag ccgccgcaag gcaaggttga
     2161 tgccgcgctg gtcaaggctg ccggcccgga aggagatcgc gtgcggaaag cgcggcccgg
     2221 cgagcaggag gtggcgcaat ccgcggtcac ctattacgcc gcggtggaca gggccgggca
     2281 ggaggtcgcc tggctttcgc tcaagccggt caccgggcgc cagcatcagc tccgcgccca
     2341 catggccatc atcggccacc ccattctcgg cgatgagaag taccagggcg accgcgatct
     2401 gcccgagggg atcgaaaaca agctgaatct gcacgcaagg cgcatcagct tcccgcatcc
     2461 gtcgggggag ggggtcgtcg acgtcacggc gccgctgcct gatcatatgc tagcgagctt
     2521 cgcggcgttc ggttttgccg aagccgatgc gaccgatgcg ggcgacagcg cgttatgatg
     2581 cggagacccg cgcccccgcc cttacatata aggaaataga gatggccgac cgcaaactct
     2641 ggttcgtgca tttcgcccaa tgggtgtccc acgtcaccgg acgccccgtc gcgttccttg
     2701 ccgccttcgc gagtatc
//
LOCUS       DATNAE010000307         2298 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3604965, whole genome shotgun sequence.
ACCESSION   DATNAE010000307 DATNAE010000000
VERSION     DATNAE010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3604965"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VN518_06865"
     CDS             <1..268
                     /locus_tag="VN518_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010684493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HXE04330.1"
                     /translation="LPTTPTLPPEREIPDGASFAEFRAQTLGYTCLAGHAGLPQISIP
                     ATEAAGCPIGLSFIGWRGGDEALLDLAGTLAHMPNASSEEGASP"
     gene            265..630
                     /locus_tag="VN518_06870"
     CDS             265..630
                     /locus_tag="VN518_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HXE04331.1"
                     /translation="MTLALPYLYLALAIVFEVLGTSALKASETFTRLVPSLVTLIAYT
                     VSFVFLALSLRTIPIGIAYAMWAGLGIVLIAMIGWLWFKQPLDVPALIGLALIICGVV
                     VVNGSHNPSRIEPLLRNAL"
     gene            661..2097
                     /locus_tag="VN518_06875"
     CDS             661..2097
                     /locus_tag="VN518_06875"
                     /inference="COORDINATES: protein motif:HMM:NF015043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HXE04332.1"
                     /translation="MLDKPAFPYGAGFRSLTTETPAPVALAVTGALPSWLKGTLLRTG
                     PSKFEVGPRTYNHWFDGLAMLHRFAIADGGVSYANRFLESKAFRAAEETGKITYAEFA
                     TDPCRTLFGRVASIFDPKLTDNCCVNVSEYAREIVAFTETTMPTRFAPDTLKTLGVFG
                     YGAALTGQISTAHPHYDAKRACHYNYIADLGMKSVYRLCRIGDDGTQFELAAIPVERP
                     AYMHSFGMSADHLILTEFPMVVNPIDLRLSGKPFIQNYRWEAERGLLFHVVEKDTGRL
                     VRTARADASFAFHHVNAFADAGGIAVDVIVYPDATIIDQLYLARLRSGAPITAVGTLT
                     RFQIPLASEAPVTRRTLAELSLELPRINYERHAGKPYRYVWGTGIAAEGDFLDCIVKI
                     DTATGASARWYAAGLYPGEPVFVPSPSAAAEDDGVLLSVVLDIDKGRSFLLVLDAASL
                     KELARAAAPHAIPFHFHGNFFESRPSLA"
BASE COUNT          371 a          795 c          675 g          457 t
ORIGIN      
        1 gctgccgacg acgccgacgc tgccgcccga gcgcgagatt cccgatggcg cctccttcgc
       61 cgagttccgc gcgcaaacgc tcggctatac ctgcctcgcc ggccacgccg gcctgccgca
      121 gatctcgatt cccgcgaccg aggccgccgg ctgcccgatc ggcctgtctt tcatcggctg
      181 gaggggcggc gacgaggcct tgctcgatct cgccggcact ctcgctcaca tgcctaacgc
      241 aagttcagaa gaaggagcga gcccgtgacc ctggccctgc cttatcttta tctcgctctc
      301 gccatcgttt tcgaggtgct cggaacctcc gctttgaagg cctccgaaac ctttacgcgt
      361 ctcgttccaa gcctggtgac cttgatcgcc tatacggtgt ccttcgtttt cctcgcgctc
      421 agcctgcgga cgatccccat tggcatcgcc tatgccatgt gggcgggcct tggcatcgtc
      481 ctgattgcca tgatcggatg gctctggttc aagcagccgc ttgacgttcc agcacttatt
      541 gggctcgccc tgatcatctg cggcgtcgtc gtcgtgaacg gttctcacaa tccctcccgc
      601 attgagccgc tcttaaggaa cgcgctctaa tctcctcgtg ttgagatcgg cgaggacgcg
      661 atgctggaca aacccgcttt cccctacggc gcaggcttcc gctcgctgac gacggagacg
      721 cccgcgcctg ttgcgctcgc cgtcaccggc gcgctgccgt cctggctgaa agggaccctg
      781 ctccgcaccg gcccatcgaa attcgaggtg ggacctcgca cctataatca ctggttcgat
      841 ggtttggcga tgctgcatcg ctttgcgatt gccgacggcg gcgtcagcta tgcgaaccgc
      901 ttcctcgaga gcaaagcctt ccgcgccgcc gaggagaccg gcaagatcac ctatgccgag
      961 ttcgccaccg acccgtgtcg cacgctgttc gggcgggtgg cgtcgatctt cgatccgaag
     1021 ctcaccgaca attgctgcgt caatgtctcg gaatatgcgc gcgaaatcgt cgccttcacc
     1081 gagacgacga tgccgacgcg cttcgcgccc gataccctga agacgctcgg cgtgttcggc
     1141 tatggcgcgg ctttgacggg acaaatctcg acggcgcacc cgcattacga cgccaagcgc
     1201 gcctgccatt acaattatat cgccgatctc ggcatgaaga gcgtctatcg cctgtgccgc
     1261 atcggcgacg acggcacgca gttcgagctt gccgccattc cggtcgagcg gccggcttac
     1321 atgcacagct tcggcatgag cgcggaccac ctcatcctca ccgagttccc catggtggtg
     1381 aacccgatcg acctcaggct gtcgggcaag cccttcatcc agaattatcg ctgggaggct
     1441 gagcgcgggc tgctgtttca cgtcgtcgag aaagacacgg gccggctggt gcgcacggcc
     1501 agggccgatg caagcttcgc cttccatcat gtcaacgcct tcgccgatgc cggcggcatt
     1561 gccgtcgatg tcatcgtcta tcccgacgcg acgattatcg atcagctcta tctggcgcgg
     1621 ctccgctccg gcgcgccgat caccgccgtc ggcacgctga cccggtttca gattccgctc
     1681 gcgagcgagg cgcccgtcac ccgccgaact ttggccgaac tgtcgctaga actgccccgt
     1741 atcaattacg agcgccatgc cggcaagccc taccgctatg tctggggcac gggcatcgcg
     1801 gccgaaggcg atttcctcga ttgcatcgtc aagatcgaca ccgccaccgg cgccagcgcc
     1861 cgctggtacg cggcgggact ctatccgggc gagccggtgt tcgtgccctc gccctccgcc
     1921 gcagccgagg acgacggggt gctgctgtcg gtggtgctcg acatcgacaa gggccgctcc
     1981 ttcctgctcg tgctcgatgc cgcgagccta aaggagctgg cacgggcagc ggcgccgcat
     2041 gccatcccct tccattttca tggcaatttc ttcgagagca ggcccagctt ggcttgactt
     2101 gggggccgtc ctcgccgaca ttccggccgg aattcaaggc agctccttaa ccgagccgcc
     2161 gccctgaaca tctttggggt gctaccggcc tctggcctac ttgagccctg cgctcaagta
     2221 gcgcgaagtg cggtagcgca aacctaaagg ggagtcaggg gggtcaacac ccgccagcgt
     2281 tatcgcccgc gggtgcgt
//
LOCUS       DATNAE010000308         2769 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U11153
            k141_3608097, whole genome shotgun sequence.
ACCESSION   DATNAE010000308 DATNAE010000000
VERSION     DATNAE010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945729
            Sequence Read Archive: SRR6185312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:39:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,377
            CDSs (total)                      :: 1,368
            Genes (coding)                    :: 1,349
            CDSs (with protein)               :: 1,349
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3608097"
                     /isolate="SMAG_U11153"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(281..1540)
                     /locus_tag="VN518_06880"
     CDS             complement(281..1540)
                     /locus_tag="VN518_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HXE04333.1"
                     /translation="MTPELLLKAMFQAAVDAALPSLCVPAHLPKRPKGRTVVIGAGKA
                     SGAMAKALEDAWEGPIEGLVVTRYGYRVPTSRLEVVEAAHPVPDAAGREAARRIHELV
                     HGLTADDLVLCLISGGGSALLALPPEGLTLEDKQAVNKALLKSGATISEMNTVRKHLS
                     AIKGGRLAAAAAPAKVVALMISDVPGDDPSIIASGPTVPDPSTNKDALGIIEKYGIDV
                     PKAVLERLRKADETPKPGDKRLARVENIMIATPQASLEAAAEVARKAGVTPVILGDSI
                     EGESRDVALVHAGIARQCALRGQPEKPPCVLISGGETTITLKGKGKGGRNTEFLLALA
                     IALDGMKGIYALAGDTDGVDGSEDNAGALIYPDTLARASKAGISAKAMLADNDPWTFF
                     NAIGDLLVTGPTLTNVNDFRAVLIEKT"
     gene            1770..>2769
                     /locus_tag="VN518_06885"
     CDS             1770..>2769
                     /locus_tag="VN518_06885"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006725690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HXE04334.1"
                     /translation="MRPILEVAKDKLGIDAEDLIPYGHYKAKVSLDYIASLRGRPNGK
                     LVLVTAITPTPAGEGKTTTTVGLGDALNEIGKKAIICIREPSLGPCFGVKGGAAGGGY
                     AQVVPMEDINLHFTGDFHAIGAANNLLAALIDNHVYWGNKLDIDTRRVTWRRAVDMND
                     RALRDIVTSLGGAANGYPREAGFDITVASEVMAIFCLSSDLADLQRRLGQIQIGQTRD
                     KKAVTARDLSAAGSMAALLKDALAPNFVQTLENNPAFIHGGPFANIAHGCNSVIATKA
                     ALKLGDYVVTEAGFGADLGAEKFFDIKCRKAGLKPSCVVIVATIRALKMHGGVAKHD"
BASE COUNT          486 a          906 c          896 g          481 t
ORIGIN      
        1 gcgactgccg agaggttggt gaggagcttt ggattgccgg tgacggcgcc gagatagtcg
       61 agagtgatct gctcgagacc aggcgcagcc atgctttacg ccccttacgc caaaattcct
      121 catcgctgcc cgcccggcca aatcggcttg cgtgcgagcc tgtcgcttgc tctcaaaagt
      181 agcaggcgga acagtgaagt ggaaggcgga cgcggcaaaa acggccggcc tcgcgcccgg
      241 tcttatggtc gctggcggtc ggcgcttacg gcgagcgcgc tcaggtcttc tcgatcagaa
      301 cggcgcggaa gtcgttgacg ttggtgagcg tcggaccggt caccaagaga tcgccgatcg
      361 cattgaagaa agtccacggg tcattgtcgg cgagcatcgc cttggcgctg atcccggcct
      421 tggacgcgcg ggcgagtgtg tcgggataga tcagcgcgcc ggcattgtcc tcgctgccat
      481 ccacgccgtc agtgtctccg gcgagggcat aaatgccctt catgccgtcg agcgctattg
      541 ccagcgcgag caggaactcg gtgttgcgcc cgcctttgcc tttgcccttc agcgtgatcg
      601 tcgtctcgcc gccggagatc agcacgcatg gcggcttctc cggctgacca cgcagcgcgc
      661 attgccgtgc gatgccggca tgcaccagcg ccacgtcgcg cgactcgccc tcgatcgaat
      721 cgccgagaat gaccggggtg acgcccgcct tgcgcgcgac ctccgccgcg gcttcgagcg
      781 aggcttgcgg cgtggcgatc atgatgttct cgacacgcgc cagccgcttg tcgccgggct
      841 tcggcgtctc gtcggctttg cgcaggcgtt cgagcacggc cttcggcacg tcgatgccat
      901 atttctcgat gatgccgagc gcgtccttgt tggtggaggg atccggcacg gtcgggcctg
      961 aagcgatgat cgagggatcg tcgcccggca cgtcggagat catcagcgcg accaccttgg
     1021 cgggagcggc cgccgcggct agcctgccgc ctttgatggc ggagagatgc ttccgcaccg
     1081 tgttcatctc ggagatggtc gcgcccgatt tgagcagcgc cttgttcacc gcctgcttgt
     1141 cttcgagcgt gagcccttcc ggcggcagcg cgagcagggc ggacccgccg cccgagatca
     1201 ggcaaagcac gagatcgtcc gccgtcaatc cgtgcaccaa ttcgtggatg cgccgcgcgg
     1261 cctcgcggcc ggcagcgtcg gggacgggat gggcggcctc gaccacttcg agccggctcg
     1321 tgggcacgcg atagccgtag cgcgtcacca ccagtccctc gatcggcccc tcccaggcat
     1381 cttccagcgc cttggccatc gcgcccgagg ccttgccggc gccgatcacg acagtgcgcc
     1441 ccttcggccg cttgggcagg tgagccggca cgcagagcga gggtagcgcg gcgtctaccg
     1501 cagcctggaa catggccttg agcagcagtt cgggcgtcat tgtccggctt gtcctccccc
     1561 ttacggccga tgaaactgca cagacgattg caataaggca agcgaatgaa ctactttgct
     1621 cattcctttt gcaagcgaag ccgcaaagcc ccggggaccg aggagttatg tcagtacaac
     1681 cgcgtgaaac tgccgaaaaa ccgggcaggc gttcggacga gcagcacaag aagcccaaat
     1741 cggatatcga gatcagccag gccgcgcaca tgcggccgat cctcgaagtg gcgaaggaca
     1801 aactcggcat cgatgccgag gacttgattc cctacgggca ttacaaggcg aaagtctcgc
     1861 tcgactatat cgcctcgctg aggggcaggc ccaacggcaa actcgtcctc gtcactgcca
     1921 tcacccccac gccggccggc gagggcaaga ccacgaccac ggtcgggctt ggcgatgcgc
     1981 tgaacgagat cggcaagaag gccatcatct gcattcgcga gccgtcgctt gggccctgct
     2041 tcggggtgaa ggggggcgcc gccggcggcg gctacgcgca ggtcgtgccg atggaggaca
     2101 tcaacctgca tttcaccggc gacttccacg ccatcggtgc cgccaacaat ctgctcgcgg
     2161 cgttgatcga caatcatgtc tactggggca acaagctcga catcgacacc cgccgtgtca
     2221 cctggcggcg cgccgtcgat atgaacgacc gggcgctgcg cgacatcgtc acctcgctgg
     2281 ggggcgcggc caacggttat ccgcgcgaag ccggcttcga tatcaccgtg gcgtcggaag
     2341 tcatggcgat cttctgcctg tccagcgacc tggccgatct tcagcgtcga ctcggccaga
     2401 ttcaaatcgg ccagacccgc gataagaaag ccgtcaccgc cagggacctc tcggccgcgg
     2461 gctcgatggc ggcgctgctc aaggacgcgc tcgcgcccaa tttcgtgcag acgctcgaga
     2521 acaacccggc cttcatccat ggcgggccgt tcgccaacat cgcccacggc tgcaactcgg
     2581 tgattgccac caaggccgcg ctcaagctcg gcgactatgt ggtgaccgag gcggggttcg
     2641 gcgccgatct cggcgccgag aaattcttcg acatcaaatg ccgcaaggcg gggctgaagc
     2701 cgtcttgcgt ggtgatcgtg gcgaccatcc gcgcgctcaa gatgcatggc ggggtggcca
     2761 agcacgatc
//
